LOCUS       DAVKWS010000001       120187 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKWS010000001 DAVKWS010000000
VERSION     DAVKWS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 120187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.001"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(223..1785)
                     /gene="guaA"
                     /locus_tag="VW852_000001"
     CDS             complement(223..1785)
                     /gene="guaA"
                     /locus_tag="VW852_000001"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV0271217.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1927..2625
                     /locus_tag="VW852_000002"
     CDS             1927..2625
                     /locus_tag="VW852_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0271218.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2668..3564)
                     /locus_tag="VW852_000003"
     CDS             complement(2668..3564)
                     /locus_tag="VW852_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0271219.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATTKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIILAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3573..4508)
                     /locus_tag="VW852_000004"
     CDS             complement(3573..4508)
                     /locus_tag="VW852_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV0271220.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKVGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4505..5230)
                     /locus_tag="VW852_000005"
     CDS             complement(4505..5230)
                     /locus_tag="VW852_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV0271221.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5314..5949)
                     /locus_tag="VW852_000006"
     CDS             complement(5314..5949)
                     /locus_tag="VW852_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV0271222.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5951..6778)
                     /locus_tag="VW852_000007"
     CDS             complement(5951..6778)
                     /locus_tag="VW852_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0271223.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQAI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6800..7136)
                     /locus_tag="VW852_000008"
                     /pseudo
     CDS             complement(6800..7136)
                     /locus_tag="VW852_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(7137..7853)
                     /locus_tag="VW852_000009"
     CDS             complement(7137..7853)
                     /locus_tag="VW852_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV0271224.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWFKQADLAIG
                     DFEETINKDHYLAGYPLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGYP
                     HVVEPSETVEKDGDKKLIVY"
     gene            8366..8977
                     /locus_tag="VW852_000010"
     CDS             8366..8977
                     /locus_tag="VW852_000010"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0271225.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEKVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9022..9750
                     /gene="rlmB"
                     /locus_tag="VW852_000011"
     CDS             9022..9750
                     /gene="rlmB"
                     /locus_tag="VW852_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV0271226.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9789..10700)
                     /gene="trxB"
                     /locus_tag="VW852_000012"
     CDS             complement(9789..10700)
                     /gene="trxB"
                     /locus_tag="VW852_000012"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV0271227.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10766..10990)
                     /locus_tag="VW852_000013"
     CDS             complement(10766..10990)
                     /locus_tag="VW852_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV0271228.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11068..11811)
                     /locus_tag="VW852_000014"
     CDS             complement(11068..11811)
                     /locus_tag="VW852_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0271229.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMPFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11811..12611)
                     /locus_tag="VW852_000015"
     CDS             complement(11811..12611)
                     /locus_tag="VW852_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0271230.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12769..13125)
                     /locus_tag="VW852_000016"
     CDS             complement(12769..13125)
                     /locus_tag="VW852_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0271231.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13138..13650)
                     /locus_tag="VW852_000017"
     CDS             complement(13138..13650)
                     /locus_tag="VW852_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV0271232.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13650..14144)
                     /locus_tag="VW852_000018"
     CDS             complement(13650..14144)
                     /locus_tag="VW852_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271233.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGKRLAYQ
                     KLKK"
     gene            14279..14725
                     /locus_tag="VW852_000019"
     CDS             14279..14725
                     /locus_tag="VW852_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV0271234.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            14722..15369
                     /locus_tag="VW852_000020"
     CDS             14722..15369
                     /locus_tag="VW852_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV0271235.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(15390..15971)
                     /gene="pdxT"
                     /locus_tag="VW852_000021"
     CDS             complement(15390..15971)
                     /gene="pdxT"
                     /locus_tag="VW852_000021"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV0271236.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15972..16847)
                     /gene="pdxS"
                     /locus_tag="VW852_000022"
     CDS             complement(15972..16847)
                     /gene="pdxS"
                     /locus_tag="VW852_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV0271237.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16999..18378)
                     /locus_tag="VW852_000023"
     CDS             complement(16999..18378)
                     /locus_tag="VW852_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV0271238.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18672..19595
                     /locus_tag="VW852_000024"
     CDS             18672..19595
                     /locus_tag="VW852_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV0271239.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVENLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19655..20260
                     /locus_tag="VW852_000025"
     CDS             19655..20260
                     /locus_tag="VW852_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0271240.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEETDGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20279..21523
                     /locus_tag="VW852_000026"
     CDS             20279..21523
                     /locus_tag="VW852_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0271241.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21640..21897)
                     /locus_tag="VW852_000027"
     CDS             complement(21640..21897)
                     /locus_tag="VW852_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV0271242.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(21939..23975)
                     /locus_tag="VW852_000028"
     CDS             complement(21939..23975)
                     /locus_tag="VW852_000028"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV0271243.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24235..25152)
                     /gene="glyQ"
                     /locus_tag="VW852_000029"
     CDS             complement(24235..25152)
                     /gene="glyQ"
                     /locus_tag="VW852_000029"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV0271244.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25346..26107)
                     /locus_tag="VW852_000030"
     CDS             complement(25346..26107)
                     /locus_tag="VW852_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0271245.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26380..27222)
                     /locus_tag="VW852_000031"
     CDS             complement(26380..27222)
                     /locus_tag="VW852_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV0271246.1"
                     /translation="MDTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREELFVTTKLWNSQQTYEQARQAFEESLEKLGLDYLD
                     LYLIHWPNPKPLRENDQWKIRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     KILPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27298..27375)
                     /locus_tag="VW852_000032"
                     /pseudo
     CDS             complement(27298..27375)
                     /locus_tag="VW852_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(27478..28869)
                     /locus_tag="VW852_000033"
     CDS             complement(27478..28869)
                     /locus_tag="VW852_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV0271247.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKDQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29104..29481)
                     /locus_tag="VW852_000034"
                     /pseudo
     CDS             complement(29104..29481)
                     /locus_tag="VW852_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29714..30691
                     /locus_tag="VW852_000035"
     CDS             29714..30691
                     /locus_tag="VW852_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0271248.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGVRFEAEK"
     gene            30688..31770
                     /locus_tag="VW852_000036"
     CDS             30688..31770
                     /locus_tag="VW852_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0271249.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31834..32882)
                     /locus_tag="VW852_000037"
                     /pseudo
     CDS             complement(31834..32882)
                     /locus_tag="VW852_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(33266..34462)
                     /gene="tuf"
                     /locus_tag="VW852_000038"
     CDS             complement(33266..34462)
                     /gene="tuf"
                     /locus_tag="VW852_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0271250.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(35373..36242)
                     /locus_tag="VW852_000039"
     CDS             complement(35373..36242)
                     /locus_tag="VW852_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0271251.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(36455..36985)
                     /locus_tag="VW852_000040"
     CDS             complement(36455..36985)
                     /locus_tag="VW852_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0271252.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKKMKNNRF"
     gene            complement(36943..38049)
                     /locus_tag="VW852_000041"
     CDS             complement(36943..38049)
                     /locus_tag="VW852_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV0271253.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(38160..38414)
                     /locus_tag="VW852_000042"
     CDS             complement(38160..38414)
                     /locus_tag="VW852_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271254.1"
                     /translation="MLNVDQDFMSISKSNKSDSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(38772..39305)
                     /locus_tag="VW852_000043"
     CDS             complement(38772..39305)
                     /locus_tag="VW852_000043"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV0271255.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(39292..39618)
                     /locus_tag="VW852_000044"
     CDS             complement(39292..39618)
                     /locus_tag="VW852_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0271256.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            39852..41570
                     /locus_tag="VW852_000045"
     CDS             39852..41570
                     /locus_tag="VW852_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV0271257.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            41681..42028
                     /locus_tag="VW852_000046"
     CDS             41681..42028
                     /locus_tag="VW852_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0271258.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(42306..43142)
                     /locus_tag="VW852_000047"
     CDS             complement(42306..43142)
                     /locus_tag="VW852_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271259.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(43155..43784)
                     /locus_tag="VW852_000048"
     CDS             complement(43155..43784)
                     /locus_tag="VW852_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0271260.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(43794..44435)
                     /locus_tag="VW852_000049"
     CDS             complement(43794..44435)
                     /locus_tag="VW852_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0271261.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(44581..45810)
                     /locus_tag="VW852_000050"
     CDS             complement(44581..45810)
                     /locus_tag="VW852_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV0271262.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNSS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(45800..46966)
                     /locus_tag="VW852_000051"
     CDS             complement(45800..46966)
                     /locus_tag="VW852_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0271263.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            47110..48123
                     /locus_tag="VW852_000052"
     CDS             47110..48123
                     /locus_tag="VW852_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV0271264.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNEFHVPEAVCIR
                     FAP"
     gene            complement(48168..48587)
                     /locus_tag="VW852_000053"
     CDS             complement(48168..48587)
                     /locus_tag="VW852_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV0271265.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(48598..50004)
                     /gene="atpD"
                     /locus_tag="VW852_000054"
     CDS             complement(48598..50004)
                     /gene="atpD"
                     /locus_tag="VW852_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV0271266.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(50090..50968)
                     /locus_tag="VW852_000055"
     CDS             complement(50090..50968)
                     /locus_tag="VW852_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV0271267.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(50984..52489)
                     /locus_tag="VW852_000056"
     CDS             complement(50984..52489)
                     /locus_tag="VW852_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV0271268.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(52504..53040)
                     /locus_tag="VW852_000057"
     CDS             complement(52504..53040)
                     /locus_tag="VW852_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV0271269.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(53040..53534)
                     /gene="atpF"
                     /locus_tag="VW852_000058"
     CDS             complement(53040..53534)
                     /gene="atpF"
                     /locus_tag="VW852_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV0271270.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(53548..54264)
                     /gene="atpB"
                     /locus_tag="VW852_000059"
     CDS             complement(53548..54264)
                     /gene="atpB"
                     /locus_tag="VW852_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV0271271.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNILEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(54299..54499)
                     /locus_tag="VW852_000060"
     CDS             complement(54299..54499)
                     /locus_tag="VW852_000060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV0271272.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(54574..55524)
                     /locus_tag="VW852_000061"
                     /pseudo
     CDS             complement(54574..55524)
                     /locus_tag="VW852_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(55958..56356)
                     /locus_tag="VW852_000062"
     CDS             complement(55958..56356)
                     /locus_tag="VW852_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271273.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(56729..57211)
                     /gene="greA"
                     /locus_tag="VW852_000063"
     CDS             complement(56729..57211)
                     /gene="greA"
                     /locus_tag="VW852_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV0271274.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(57282..58937)
                     /gene="mltG"
                     /locus_tag="VW852_000064"
     CDS             complement(57282..58937)
                     /gene="mltG"
                     /locus_tag="VW852_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV0271275.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTINELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTDIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(59018..59512)
                     /locus_tag="VW852_000065"
     CDS             complement(59018..59512)
                     /locus_tag="VW852_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271276.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(59509..59973)
                     /locus_tag="VW852_000066"
     CDS             complement(59509..59973)
                     /locus_tag="VW852_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271277.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDCKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(59983..61317)
                     /locus_tag="VW852_000067"
     CDS             complement(59983..61317)
                     /locus_tag="VW852_000067"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV0271278.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(61328..61945)
                     /locus_tag="VW852_000068"
     CDS             complement(61328..61945)
                     /locus_tag="VW852_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0271279.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(61993..65091)
                     /locus_tag="VW852_000069"
     CDS             complement(61993..65091)
                     /locus_tag="VW852_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0271280.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(65184..66350)
                     /locus_tag="VW852_000070"
     CDS             complement(65184..66350)
                     /locus_tag="VW852_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0271281.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(66358..67452)
                     /locus_tag="VW852_000071"
     CDS             complement(66358..67452)
                     /locus_tag="VW852_000071"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0271282.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(67609..68154)
                     /locus_tag="VW852_000072"
     CDS             complement(67609..68154)
                     /locus_tag="VW852_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271283.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            complement(68190..68729)
                     /locus_tag="VW852_000073"
     CDS             complement(68190..68729)
                     /locus_tag="VW852_000073"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271284.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            complement(68738..69272)
                     /locus_tag="VW852_000074"
                     /pseudo
     CDS             complement(68738..69272)
                     /locus_tag="VW852_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(69444..71402)
                     /locus_tag="VW852_000075"
     CDS             complement(69444..71402)
                     /locus_tag="VW852_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271285.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(71605..73227)
                     /locus_tag="VW852_000076"
     CDS             complement(71605..73227)
                     /locus_tag="VW852_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0271286.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            73333..74778
                     /locus_tag="VW852_000077"
     CDS             73333..74778
                     /locus_tag="VW852_000077"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV0271287.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHIPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(74824..75018)
                     /locus_tag="VW852_000078"
     CDS             complement(74824..75018)
                     /locus_tag="VW852_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0271288.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(75124..75726)
                     /locus_tag="VW852_000079"
     CDS             complement(75124..75726)
                     /locus_tag="VW852_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV0271289.1"
                     /translation="MFGELIRYLDQYEDVILRGIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFY"
     gene            complement(75751..76263)
                     /locus_tag="VW852_000080"
     CDS             complement(75751..76263)
                     /locus_tag="VW852_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV0271290.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(76350..77285)
                     /locus_tag="VW852_000081"
     CDS             complement(76350..77285)
                     /locus_tag="VW852_000081"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV0271291.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(77356..77655)
                     /locus_tag="VW852_000082"
     CDS             complement(77356..77655)
                     /locus_tag="VW852_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV0271292.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(77645..78394)
                     /locus_tag="VW852_000083"
     CDS             complement(77645..78394)
                     /locus_tag="VW852_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV0271293.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(78448..78807)
                     /locus_tag="VW852_000084"
     CDS             complement(78448..78807)
                     /locus_tag="VW852_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV0271294.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(78809..80209)
                     /locus_tag="VW852_000085"
                     /pseudo
     CDS             complement(78809..80209)
                     /locus_tag="VW852_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
     gene            complement(80504..80743)
                     /locus_tag="VW852_000086"
     CDS             complement(80504..80743)
                     /locus_tag="VW852_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV0271295.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(80775..81245)
                     /gene="ssbA"
                     /locus_tag="VW852_000087"
     CDS             complement(80775..81245)
                     /gene="ssbA"
                     /locus_tag="VW852_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV0271296.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(81257..81547)
                     /locus_tag="VW852_000088"
     CDS             complement(81257..81547)
                     /locus_tag="VW852_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV0271297.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(81700..83046)
                     /gene="asnS"
                     /locus_tag="VW852_000089"
     CDS             complement(81700..83046)
                     /gene="asnS"
                     /locus_tag="VW852_000089"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV0271298.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(83062..83427)
                     /locus_tag="VW852_000090"
     CDS             complement(83062..83427)
                     /locus_tag="VW852_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0271299.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMTFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(83420..84607)
                     /locus_tag="VW852_000091"
     CDS             complement(83420..84607)
                     /locus_tag="VW852_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0271300.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGASENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(84604..85092)
                     /locus_tag="VW852_000092"
     CDS             complement(84604..85092)
                     /locus_tag="VW852_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV0271301.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEKADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            85413..85961
                     /locus_tag="VW852_000093"
     CDS             85413..85961
                     /locus_tag="VW852_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0271302.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTYPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(86239..86967)
                     /gene="yaaA"
                     /locus_tag="VW852_000094"
     CDS             complement(86239..86967)
                     /gene="yaaA"
                     /locus_tag="VW852_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV0271303.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(87046..87210)
                     /locus_tag="VW852_000095"
     CDS             complement(87046..87210)
                     /locus_tag="VW852_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV0271304.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(87377..88972)
                     /locus_tag="VW852_000096"
     CDS             complement(87377..88972)
                     /locus_tag="VW852_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271305.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRVYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            complement(88984..89394)
                     /locus_tag="VW852_000097"
     CDS             complement(88984..89394)
                     /locus_tag="VW852_000097"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0271306.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(89510..90301)
                     /gene="yghU"
                     /locus_tag="VW852_000098"
     CDS             complement(89510..90301)
                     /gene="yghU"
                     /locus_tag="VW852_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV0271307.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(90314..93010)
                     /locus_tag="VW852_000099"
     CDS             complement(90314..93010)
                     /locus_tag="VW852_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV0271308.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            93314..94498
                     /gene="mntE"
                     /locus_tag="VW852_000100"
     CDS             93314..94498
                     /gene="mntE"
                     /locus_tag="VW852_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV0271309.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKTAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(94641..96512)
                     /locus_tag="VW852_000101"
     CDS             complement(94641..96512)
                     /locus_tag="VW852_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0271310.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(96509..97693)
                     /locus_tag="VW852_000102"
     CDS             complement(96509..97693)
                     /locus_tag="VW852_000102"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV0271311.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(97705..98472)
                     /locus_tag="VW852_000103"
     CDS             complement(97705..98472)
                     /locus_tag="VW852_000103"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV0271312.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     DIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(98749..99597)
                     /locus_tag="VW852_000104"
     CDS             complement(98749..99597)
                     /locus_tag="VW852_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0271313.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(99599..99973)
                     /locus_tag="VW852_000105"
     CDS             complement(99599..99973)
                     /locus_tag="VW852_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV0271314.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(100047..101399)
                     /locus_tag="VW852_000106"
     CDS             complement(100047..101399)
                     /locus_tag="VW852_000106"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV0271315.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(101423..102202)
                     /locus_tag="VW852_000107"
     CDS             complement(101423..102202)
                     /locus_tag="VW852_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV0271316.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(102189..103046)
                     /locus_tag="VW852_000108"
     CDS             complement(102189..103046)
                     /locus_tag="VW852_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV0271317.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            103182..104150
                     /locus_tag="VW852_000109"
     CDS             103182..104150
                     /locus_tag="VW852_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0271318.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(104184..105095)
                     /gene="whiA"
                     /locus_tag="VW852_000110"
     CDS             complement(104184..105095)
                     /gene="whiA"
                     /locus_tag="VW852_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV0271319.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(105092..106069)
                     /locus_tag="VW852_000111"
     CDS             complement(105092..106069)
                     /locus_tag="VW852_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV0271320.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(106066..106956)
                     /gene="rapZ"
                     /locus_tag="VW852_000112"
     CDS             complement(106066..106956)
                     /gene="rapZ"
                     /locus_tag="VW852_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV0271321.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(107008..107388)
                     /locus_tag="VW852_000113"
     CDS             complement(107008..107388)
                     /locus_tag="VW852_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV0271322.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(107399..107986)
                     /locus_tag="VW852_000114"
     CDS             complement(107399..107986)
                     /locus_tag="VW852_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV0271323.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(107995..109227)
                     /gene="clpX"
                     /locus_tag="VW852_000115"
     CDS             complement(107995..109227)
                     /gene="clpX"
                     /locus_tag="VW852_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV0271324.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRMGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(109259..109429)
                     /locus_tag="VW852_000116"
     CDS             complement(109259..109429)
                     /locus_tag="VW852_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271325.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(109429..109935)
                     /locus_tag="VW852_000117"
     CDS             complement(109429..109935)
                     /locus_tag="VW852_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV0271326.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(110065..110583)
                     /locus_tag="VW852_000118"
     CDS             complement(110065..110583)
                     /locus_tag="VW852_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV0271327.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(111079..112584)
                     /gene="lytC"
                     /locus_tag="VW852_000119"
     CDS             complement(111079..112584)
                     /gene="lytC"
                     /locus_tag="VW852_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV0271328.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(112622..113380)
                     /locus_tag="VW852_000120"
     CDS             complement(112622..113380)
                     /locus_tag="VW852_000120"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV0271329.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(113478..114155)
                     /locus_tag="VW852_000121"
     CDS             complement(113478..114155)
                     /locus_tag="VW852_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV0271330.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(114164..115108)
                     /locus_tag="VW852_000122"
     CDS             complement(114164..115108)
                     /locus_tag="VW852_000122"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV0271331.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(115290..115802)
                     /locus_tag="VW852_000123"
     CDS             complement(115290..115802)
                     /locus_tag="VW852_000123"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV0271332.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(115889..116647)
                     /locus_tag="VW852_000124"
     CDS             complement(115889..116647)
                     /locus_tag="VW852_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0271333.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAIDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            116855..117001
                     /locus_tag="VW852_000125"
     CDS             116855..117001
                     /locus_tag="VW852_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271334.1"
                     /translation="MEIKEKTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(117148..118278)
                     /gene="ugpC"
                     /locus_tag="VW852_000126"
     CDS             complement(117148..118278)
                     /gene="ugpC"
                     /locus_tag="VW852_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV0271335.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(118561..118646)
                     /locus_tag="VW852_000127"
     tRNA            complement(118561..118646)
                     /locus_tag="VW852_000127"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118610..118612),aa:Leu,seq:aag)
     gene            complement(118728..119303)
                     /locus_tag="VW852_000128"
     CDS             complement(118728..119303)
                     /locus_tag="VW852_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0271336.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(119303..120091)
                     /gene="codY"
                     /locus_tag="VW852_000129"
     CDS             complement(119303..120091)
                     /gene="codY"
                     /locus_tag="VW852_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV0271337.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT        35165 a        26943 c        22034 g        36045 t
ORIGIN      
        1 aaaaaacttg attttggagt agttataaaa actcattcag ataatttatt atttgatttt
       61 gatacgatcc ctgaaaatat aatagtagtt gaccattttg aacttacaat cgaaaaatgg
      121 ttaagtaaaa gtcaacagtt actatagttt gcaaattatt tacagaaaga acaaaaaatg
      181 ctctgaaaca ctgatttcaa ggcatttttt gttagtttaa aattactccc actcaactgt
      241 tgcaggtggt ttacttgtaa tatcgtagac gatacggtta acatgatcca cttcatttac
      301 gatacgtact gagatttttt gaagtacttc ccatggaatt ttggcaaaat cagcagtcat
      361 accatcgata gaagtgatag cacggattgc aatcgtgtag tcatacgtac gaccgtcacc
      421 cataacaccg actgaacgaa cgcctgtgtt aacagtgaag tattgccaaa tatcgcggtc
      481 aagtccagct ttagcgattt cttcacgaag aatagcgtct gattcacgaa cggtttcaag
      541 tttctcttca gtgatttcac ccatgacacg gatagcaagt cctggtcctg ggaatggttg
      601 gcgccatacg atatggtctg gcataccaag ctctgtacca agagcacgaa cttcatcctt
      661 gtaaagagta ttgagtggtt caatcaattc aaactgcata tcttctggaa gaccacccac
      721 gttgtggtgt gacttgatag tttgagctgt atccgtacca gactcgataa catctgtata
      781 taaagtacct tgagcaagga atttcacatc tttgagcttg cttgcttcgt catcgaatac
      841 atagacaaac tcgttaccga tgattttacg tttttgttca gggtcagaaa cgccagcaag
      901 tttgtcaagg aaacgtttag cagcgtctgc tttgacgata ttcaaaccaa acttaccacc
      961 gagcatgtcc ataacttgat cagcttcgcc tttacgaaga agaccgtggt ctacgaagat
     1021 acagatcaat tgatcgccaa tcgctttttg gagaagaacc ccaacgacag atgagtcaac
     1081 accacctgat agaccaagaa ggacacgttt atcaccgacg gtttcacgaa tttttttgat
     1141 ctgcatgtca atgaaattat ccattgacca gtcaccttta gccttacaaa tgttaagggc
     1201 aaagttacga aggatatcat ttccgtatac agaatgacga acttctgggt ggaattggat
     1261 accgtaaatg tgtttatctg ggttttcgat ggctgcgtat gggcagtcag ctgatgtacc
     1321 tgtacgaaca aagtcagcag gaatctcagt aaccgcatca ccatggctca tcaaaacagt
     1381 ctgttcatca ggtgttgatt caaaaagcgc tgatggtgtg tgagttaggg ttgattgacc
     1441 gtattcacga tttccagcat cacctgcagg aacaactttt cctccaagtt tatgggtcaa
     1501 taactgcata ccataacaga ttcccaaaat tggaattccg agttcgaaga tttctgggtc
     1561 aatatcaaat gaaccatctt catatacaga atttggacca cctgatagaa taattcctac
     1621 aggattgact tcacgaactt cagcagctga aattttatgg ctttttagtt ctgaaaaaac
     1681 accaatctca cggatacggc gtgaaatcag ctggttgtac tggctaccat agtccaatac
     1741 gatgattttt tctacatctt gcaaatcagt tgaaatgttg ctcatctttt tcctttctca
     1801 aaagaaatat cttttctcaa tattctatca caaataaggg cgaattacca actaaacaag
     1861 gcgaagtttg actttttcta gtttattggg gtacaataga gaaaagatag aaatgaagtg
     1921 aaaaatatgc tacctgctta tatgaaaatc catgatcaga taaaaaagga tattgacgag
     1981 caccgttggg ctattggtga gaggcttccc agtgaaagag atttagctga gcagtttgcg
     2041 gtcagtcgca tgaccctccg ccaagccgta tctctattag tcgaagaagg cgtcttagag
     2101 cgccgtgtag gaagcggcac ctttgtttcc agtactcgag tacaagaaaa gatgcgaggg
     2161 acaaccagtt ttactgaaat tgtcaaatcc caaggtaaag ttccctctag ccagctcatt
     2221 tcctacaaaa aaaccattcc caatgagcaa gaagttgcca agctaggaat ttttccaacg
     2281 gacaatatta tccgaatgga gcgggtccgc tatgccgacc aagttcccct agtttatgaa
     2341 gttgcttcta ttcctgaaaa attcattaag gactttaaaa aagaagaaat caccagtcat
     2401 ttcttccaaa ccttgcaaaa acatggctat cgtatcggta aatcccaaca gaccatctat
     2461 gctcgccttg ctaaagaaaa gattgcccac tatttggaag ttgaaaaagg acatgctatt
     2521 cttggattga cccagatttc ctacctagaa gatggtactg cttttgaata cgtaaaaagt
     2581 caatatgtag gcgaacgctt tgaattttat cttgaaaata attagattac cacgcaaaaa
     2641 ctctctgtca cggacaaaga gtctttttta tttttctaag aataaatctt tcaaagaaat
     2701 caaagttaca gcaaacaata tcattgccat accaagaaaa tcaatgggat aaaattgttc
     2761 attcattatt aagaaggcaa agaaaatcgc cgatattggc tcaattgaag ccaacaagct
     2821 tgacttgacc ggtcctatca gactggctcc tttaaggaaa gctgtatagg caaagacagt
     2881 cccgataagg ataatgcctg caaacgcaag gagaaaatca agactagtcg ggatatcggc
     2941 ctgtagaacc cctgtaaaag gaagggcgac caaacctgct atgaccattc ccacaccaat
     3001 gaccaagctg ctcccccact ttttaatcaa ggctatgggt aaaatgatat acagagcata
     3061 agtcaaggca gaaaagagac cccagaacag gccagcaggt gtcatggata actggtccaa
     3121 ctgcccatgt gttgcgatca ggaaggttcc tccgatggcc aatatgatgg aaactatctc
     3181 tcccagtgtc ggtgccaccc tatccttgat acagctataa attaaaattc cgacaggaca
     3241 aacatactga agcaccgtcg ctgttcccgc attggtctcc tgaatagcag acagataggc
     3301 gaattggttg agaaaaagac caatcagagc aaaaataaga agagacagca aactctttct
     3361 atcctttaaa aagaccagta ttttatcctt tgtagtagca taagccaaga gcatgagaat
     3421 tccaccagcg attaaaagac gcaagttggt caagaccaga gccgaaattc cgtgtgccat
     3481 taggtattgg ccactcgttc ctgacaaccc ccaagcaata ccagccacaa ctgttagtaa
     3541 agtccctttt aaactatttg acatgtctct ccttactctt ctttgcgaat caagttcatc
     3601 acttgattgc ggtctaatat ccattgagct ctctcgtcct tttcataggt acgattcgat
     3661 aggaaaatag tggcttcttg cttctgacga ttccacatga taaaggtacc tgtatagccc
     3721 gtatggtcta gccaatctcc ttccaaattc catgctaaag aacgttcctt gtcatccaaa
     3781 ggagaaaaat tttgatttaa gtctcttgca aaatcatctg ctaaatagtg ttctaaaaag
     3841 atttgtaaat cctttatagt cgaaaataaa ccagcactcc cagcatgtct acccaggaga
     3901 cgagccttgg gatcatgcac tatgcctgcc tctacacctc taactgttgg aacagcaagc
     3961 tcaactggcc caaccttggt ttccgtcatt ccccaaggtt tccagacttg atcctttaaa
     4021 atcacatcca aatcttgatt aaaaattctt tccaaaataa agcccaacaa caaaaaatgg
     4081 acatccgaat aaagaaaggc tggctgactt cgtctgttga gatgaaacat cgcttccttt
     4141 aattcagggg ctgttaaaag atcacgatta ggaataaaag gatcaaggtc tgttgcatga
     4201 gtcaagagct ggcgaatagt gatgtctgga taatcactct caggtaaaaa atctattacc
     4261 agtctatcaa tatctaattg acctatttcc cacaagaagg tacaaactgt gccaacccca
     4321 acaaccttgc tgacactagc taggtcataa actagtcctg cctcagtctg caagccatgc
     4381 tctggggcac tctggcctaa atagaactct gtccattgat tgtccttaaa atacgcaaaa
     4441 gaggctccgg gataaattcc tgcctcgatt tgttcttcta tttttttaat aatcttggtc
     4501 cacttcatac ttcttcaaac cacaattcaa cattatttct atctttacca aggaagaatt
     4561 tttcagactt aggaataaaa tattcagtag actcaaactt ctggcgaaga cttgctatgt
     4621 ctaattcatt gaccaagaac ttgagcatag ataagtccca ggtaaccgta ttgtccacag
     4681 tcaaatcctg cccctgagct ggaataaaat caagggatgc cccaatttca gatgattcca
     4741 agaaactttc gatatcagtt gggagatgta attccataga aatctcaaat ttacttaaag
     4801 aaattgatgc caaatctgtt tgaaattcag gcttttctcc tacttctact agacttgctc
     4861 tgtcatcttc cgcatgaatc aaaatcaaat catcttctgg tgagaaaatt tcaaaagcgt
     4921 agccattttg acctttatat aatcgatgaa tcgaatctgt tttagataag attccttcaa
     4981 tttctaaggg attttccacc ttgacaatca atctagctag tttttttctt ccctctacct
     5041 tacgagtacg catactggga gcttcttcta aaaccagctt ttcaagaccc gtttggtcac
     5101 ctagtgacag aaaggccgat tcttctaaca gggccttcat tccaagggtt tcaatataaa
     5161 atgtttcatt taattttcta ttattagctt ttaaagtagg aataatccgt acaatctgat
     5221 ttacattcat aaattcctcc gtcacttttt attttaaagg attttagaat tttttacaag
     5281 atattatgct caaatttaac attatttagc tatttagtaa agatagtctt tcaaggcttt
     5341 tgacaaagcc tgataaccag caatactgag gtgcagtcca tcagttgtat attctttttt
     5401 gagttggcct gcttgatctg tcaaacaatc aaatactggc acaaattcca cctgcatata
     5461 ggcagatgca agctcttgat aggcttgatt ccaattctga attttttcat tcgagcggat
     5521 atagactgcc tgctggtact tctctccctc attgacaggc aaaatggaaa gcaatttaat
     5581 ctctgtcaat ggataatcgc gagcaacgga ttgaatgata gcttcgagat tattgagagc
     5641 ctcattcaca ggaacatcct ttccgatatc atttgtccca atcagaagaa aaattttatc
     5701 tactgctcca ccatatagat gagcatcaag gttctctagt aacagtcctg tctgatagcc
     5761 acgaattcct cgattgacaa tcgtctttga agtcccaaat agctcctgta gaggataata
     5821 ctcgacaatg gaatccccaa taaaaagaat gtttggttct acaacagaaa tgtgatttag
     5881 gtgacgatac ttggtttgaa ttttttcttg ttcctttagg agccaatttt ctaataactg
     5941 tactgccact tcattctcct ttttctaacc agttttccaa gacttgataa actcctcctt
     6001 ggctgttggc tggtgctaga gcagtcgcca cagctttggc tttctcatca gcattttcca
     6061 tcgcataggc aattccagcc atttcaagca tttcaacatc attttcacta tcaccaaaag
     6121 ccatgattgc ttgggatttc aagtcccagc gcttgagtaa ttcctccaag ccccatgctt
     6181 tatgaatccc agcttggagg atatcaatgc aaccatagcc actggataca gctcggacac
     6241 ggccatcaaa gagagtattg atttcttcca aaaccgaact caaacgttcc tcaccaacaa
     6301 ccatgctcat cttgagcaca ccaccaaaga ggtcagatgt taattcatcc acaaattgca
     6361 tccgttggta gaatttttca atcatttctg gagtcataaa actttcaaga tctgtaaaaa
     6421 tcgtaccttc cttgacaaaa tcacccttca tccccgttac aacaaactgg tcctgacacg
     6481 ctcgaccctt gaaatgagcc aaagccttgt tgacaatggc gtcatcccat gtctgagcct
     6541 gaatcaattc attgttttca aaaatacgag cgccattagc aacaaccaga accactcgat
     6601 ccaccaaggg actcagtagt tgtctcatgc ggtgaatttc attgcccgtc gcaatgacaa
     6661 aacgaatgcc cctttgatcc aactgatcta aaatcttttc caatcgtggg agatcaagct
     6721 gacctctagc atccagcaag gtcccatcca tatctgtcgc aattacctta atcatcattt
     6781 actttcctct ggattcaagc taataccact tcaatcccac atgttcattc atttctttat
     6841 aggctgtatc aattcgttcc ttagtcgctt catctaactg gtcacgatga ctgccacccc
     6901 tctataaaat cttccaaaat ataagtttgg taatgataca agggatatcc ttctggtgaa
     6961 aaggttccct ttggtgttcg attgacccaa gtaggatgag ctttagctgt tccgatagtt
     7021 gtttttccat ccttcttctt gatggtgaca tccatgagaa caccacgttc agtccactta
     7081 gcattctctt catctcccat agattcaatc cgttgattgg aaatgaagtt ccccatttaa
     7141 tagacaatga gcttcttatc tccatctttt tccaccgttt cagatggttc aacaacgtga
     7201 ggataccctc caaagataat atccgctccc caatcgatca tcttgtgata aagagctttt
     7261 tgttcttcag ttggttccaa tcgatactca acacccatct gaggcatgat aatggtgata
     7321 tctgcttcct tctctgcccg ttcaacttca gcctttatct tatcttcgtt taaatctgaa
     7381 agataacgat tatagtcttc ctgagaaata tactgctcaa ttccattgaa accataggaa
     7441 taggctaaca atgcaacctt gataccattc acttccttaa tgaccagcgg agcctgatca
     7501 cgtggttcgt gcgtataaac tccgattgga gtgattccag ctttctcaat aatatcggcc
     7561 gttgaaataa ctccctcaat ttgcgaatcc aaaatatgat tatgagctaa atccagcaca
     7621 tgataacctg cctccttaat agcatccata acttcagcag gagcattaaa gagaggataa
     7681 cccgctaaat aatgatcctt attaatggtt tcttcaaaat caccaatagc taaatctgct
     7741 tgcttgaacc aaggagtcac atactcaaaa ttttcatgaa agtcataggt accgtcttct
     7801 tttttagctg aaaagaaaag tccatcgtgg tagagcaaat ccccgtgggc cataattctg
     7861 gcagtttttt cctcttccta ctcctgagac ttttgcttag ttccctcttg agaaatagta
     7921 tctcgttttt gactagtaaa gagattcctt ggaagctttc aaacaacaag accaagccca
     7981 ttgataaacc aactgctagc aagagtatcg ccacaaatcc cttattgctc cacttgcgat
     8041 aactcctaaa aagtttacca agcccttcat aaaacgaaaa gctaaaccac cctgatttcg
     8101 attttgtctt ctttgtatct tcgtcctccc tactttctta tgcaagcctt ttctttttat
     8161 tatatcacag ataagtattt ctttcacaat tgaattgaac ttcccatcta ttttctataa
     8221 atcctaaatg ccataatgct ttcaattctt gtcattttgt gatatcatgt agaggaaatg
     8281 aactaatcca cagtggctta ttccaagtat accacttggg ctttggcagt agctaactgc
     8341 actaaacata atataaggag agaaaatgtc tgcaatagaa cgtattacaa aagctgctca
     8401 cttaattgat atgaacgata ttatccgcga gggaaatcct actctacgcg cgattgctga
     8461 gaaagtcact ttccccctat ctgaccagga aatcatccta ggcgaaaaga tgatgcaatt
     8521 ccttaaacat tcccaagatc ctgtcatggc tgaaaaaatg ggactccgcg gtggtgttgg
     8581 actggctgct ccccagttag atatctcaaa acgcattatc gctgttttgg tacctaatat
     8641 tgttgaagaa ggcgaaactc cacaggaagc ctacgatttg gaagccatta tgtacaatcc
     8701 aaaaatcgtc tctcactctg ttcaagatgc tgctcttggc gaaggagaag gttgcctgtc
     8761 tgttgaccgt aacgtgcctg gctatgttgt tcgccatgcc cgcgttactg ttgactactt
     8821 tgacaaagat ggagaaaaac accgtatcaa actcaaaggc tacaactcca ttgttgttca
     8881 gcatgaaatt gaccacatta acggtatcat gttttacgat cgcatcaatg aaaaagaccc
     8941 atttgcagtt aaagatggtt tactgattct tgaataaaga aaatcccgtt gcaagacggg
     9001 gttttgtgtt ataatagagg catgaaaaca aatgatattg tctatggtgt ccacgccgtt
     9061 accgaagctc tccttgcaaa tacaggaaac aaactctacc tccaagaaga tctccgaggt
     9121 aagaatgttg agaaagtcaa ggaactagct acagaaaaga aggtgtccat ttcttggaca
     9181 tcaaaaaaat ctctctctga gatgactgaa ggtgctgttc atcaaggttt tgttctacga
     9241 gtgtctgaat ttgcctatag cgagctagat tacatccttg caaaaacacg ccaagaagaa
     9301 aatccacttc tattgattct agatggtcta accgatcccc ataatctggg ttctatcttg
     9361 cgaacagccg atgcgaccaa tgtttcaggt gtcatcattc ccaagcaccg tactgtcgga
     9421 gtaactcctg tcgttgccaa aacagccaca ggtgctattg aacacgttcc aattgcccga
     9481 gtgaccaacc tcagtcaaac cttagataaa cttaaggatg aaggtttctg gacctttgga
     9541 acggatatga acggtactcc ttgccacaag tggaatacaa aagggaaaat cgccctcatc
     9601 attggaaatg aaggaaaagg tatctctagc aacatcaaaa aacaagtcga tgagatgatt
     9661 accattccga tgaatggaca tgttcaaagc cttaatgcca gtgttgctgc ggccattctc
     9721 atgtacgaag ttttccgaaa tagactataa aaaagtttcc agtcatctga ttggaaattt
     9781 ttttatgatt aactatgttc tgtaatgaat ttataggctt cttgaccagc gatagctcca
     9841 tctccaaccg ctgttgttac ttggcgaagg tctttcaagc gaacatctcc aactgcaaag
     9901 ataccgtcga ctgcagtttt catgtggtta tctgtcacaa tccatcctgc ctgatcttgg
     9961 atattcaatt ctttaacaaa atcgctaaga gggtccaaac caacatagat aaagacacca
    10021 ccgaaggctt gttctgtcac ttgacctgtt ttcacatttt caaatacgac tgattctact
    10081 cggttttcac ccttgatttc ccttactaca gaatcccaga taaagctgat tttttcattc
    10141 gcaaaggcgc gatcttgtaa aaccttttgg gcacgaagtt ggtcacgacg gtgaacaatg
    10201 gtaacagtct tagcaaaacg agtcaagaag agggcttctt caacagctga atctccacca
    10261 ccaactacca ataaatcttg gtcacggaag aaagcaccat cacacacagc acagtaagaa
    10321 acaccacgac tgttcagttc ttcttctcca ggcactccca aaggacggtg tttagaacca
    10381 gttgctacga taactgtacg tgtttcatat gtttggtcat cagtcatcac tttcttaaaa
    10441 tcaccatggt cttcgacatt ttcaacataa ccataaatgt gctcaacacc aagattttca
    10501 agtggttcaa acatcttttc agccaattca ggtccactaa tattagcgta tcctgggtaa
    10561 ttttcgatat cagatgtatt attcatctga ccacctggca gaccaccttc aatcaaagct
    10621 acttttagat tgcttcgagc agcatacaag gccgcagtca tccctgcagg tccagcaccg
    10681 ataataatag tatcgtacat atagattcct tctttcttgt tgtaactatc tttattctaa
    10741 ctctttcttg tcaatcacgc aaggattatg ccttgaaaac aagaattaaa ctcataaccg
    10801 caaaggataa tactgggaca accaagactc caatcataat catatcatag agatgggctt
    10861 ggcgagccct ggctgtctta tcaactcccg acatggctct cagaccaatc caaatcccta
    10921 aaaaaatcag aacaaggatg gtggtcaaga tcaaactctc gaaatataaa gaaaatagtt
    10981 gcagtagcat gatttctctc atttctatct tttttaaaga gtaaactcag ctagtccaac
    11041 taactgagtt ttcctttatc tattatatca aatataagtc cgtttgtaac tagcgaagaa
    11101 ttcttttgtc cgctcttctt taggggtgtg gataatctca tccggagttc cagactcgat
    11161 gattttcccc ttatctaaga agagaatttt atccgcaact tgggctacaa agggcatgtc
    11221 atgactgacc aaaatcatgg tctgacctga cttagcagca tctgcaatag acttttctac
    11281 ttcaccgacc aattctgggt caagggctga agttggttcg tctaagagca aaacatctgg
    11341 tttcatagca agcgcacgcg ctagggcaac ccgttgcttc tgtccacctg ataaatggcg
    11401 aggataatgg ttttcacggt ccgaaagccc aaccttagcc aactcttcct tggcaatctt
    11461 agtcgcttct tggtcagata atttcttgac aacaaccaag ccttctttca cattatcaag
    11521 tgctgttcgg cgttcaaaca aattaaactg ttggaaaacc atagacaact tacggcgtag
    11581 ggcaaggatt tcttcttgag taattttaga aaaatcaact gaaaaaccat caatctgaat
    11641 agagccactg tcaggtgttt caagataatt gagactgcga agaaaggttg attttccagc
    11701 tcctgaagaa ccaatcaagg ctacaacttc ccctttttga atatccaagt ccagatgatc
    11761 caagacagtc tgtcctgaaa aggatttgct taaattcgaa atcttaatca ttaacgaagg
    11821 tctcctttca catctgtttg cactgtatca ggtgcagaaa tagccatttt tctctcgatg
    11881 aaacgaccga ggctttcaat tccgatattg actacccaat aaacaagggc aacggagatg
    11941 aagcgttcaa aatagcgata atcagctcca cctagaatct gagcttgggc aaagacttcc
    12001 acaacacccg cactaaaagc tagagatgtt cccttggtca aaccgatgag ggaattaatc
    12061 aaggttggag tagctaccac cgctgcatta ggaataatca ctcgtcgata aacttgcgct
    12121 cgggtcatac ccagactgcg tgccgcctca atctcaccag gattaactga gagaatggct
    12181 gcacgaatgg tttcactagc ataagctgcc tcattaaagg caaaggcgac aatcgcaaaa
    12241 gctgcagctg gaatcgcatt gatattgaga ccagttcccc attgctgatt gagggctttc
    12301 aaagccaaag ggattccgta gtaggtcaac atgagttgca ccaaaatcgg tgtccctttt
    12361 aagaaactaa caaagaaggc ctgcaaggga tataaaatct tgacacgatt gatcttcaca
    12421 atggcaaaaa gaagcgccaa aaccaagcca aaaagggcac caccaattgt caacataatt
    12481 gttgttggaa gttgttggac aattctaggg attccatcaa agaccgaacg taggctaaac
    12541 agcttgccat ccggaatcaa ttgcatcaag ttttggtacc aatctgatgc taaaatcgtt
    12601 gtaacattca taaaaacatc ctcttctgat aatgattcat tctaccatgc ttctgccgtc
    12661 aatgaaatta tttgctacgg tcagatacag actttgtcta cctactagtt tatcatactt
    12721 tgaaacaggg aagttatata ttttatctat caaagagata gataaaaact atttcagtcc
    12781 cagttcttca taagattttc gataaccgat ttgcttaaca gttccatttt ctactaaaat
    12841 gggccgtttt aacaacatac cgtcacttgc tagcaactca gccgcttctt ggtttgacaa
    12901 acttcctacc ttatctttta gccctaattc acggtatttg ataccactgg tgttgaaaaa
    12961 ttgcttcaat tcaaatcctg aggtttctag ccaattcaaa atgacttctt ggctaggtgt
    13021 ttcttccacg atatggacgg ctttatagtc cacacctaat tgatttaatt cttgttttgc
    13081 ttttttacaa gttgaacatt tggggtattc gataaattct aacatgtgct tctccttcta
    13141 tttgtttata tctttaaaat ctacgccttc atgctccaag agccaagctt tcttttccac
    13201 tcctgcagca tagcctgtca gacgcttgcc tgctcccaac acacgatgac aaggtactag
    13261 gatagaccaa ggattgcgtc ccactgctcc accaattgct tgagcagaag ccacttgcag
    13321 gtcttgagca atttgtccat aggtcactgt ctgaccataa ggaatgccct gtaaatagtc
    13381 ccaaactctc ttttcaaaat tcgttccgat tggcgccaag agcaagttgg ataaatcctg
    13441 aggcttgcct ttaaagtaat catctaagca agcaataact gggtctaaaa taggatgact
    13501 aacaacttct tctatcgttt catctcctag tcccctctca aaatgcttct gctcctgaac
    13561 ccaaattcca tacaaataat ggtcatcagc tacaagtgat agaataccaa ttggtgagga
    13621 gtagaggatt tttacatact tcttttgcat tatttcttta atttttgata ggccaagcgt
    13681 ttgccatcta ctggcacctt accaacttgt ttaaaaccaa gtttttcaaa aatatgttgc
    13741 ataaccttgt tttcagcatg cgtatctgag cgaaaatcaa gataatcaaa accttcaatc
    13801 aagccctcta agaaggtttg agcaactcct tttccctgca catctgctgc cacagcaata
    13861 cggtgaaaga ctagatactc tgactctcca gcttgccagt ttccctcata aatagcttca
    13921 taggcctcct ctggactctt ggtcacagca gcataggcta gcagttctcc ctcttccaag
    13981 gctacgtagg cttgccctga gatgatatca tcgataataa catctgcatt tggatagcca
    14041 ttttgccact ggttactacc agcatctgct aaacattttt tggcatcctc tatcacctgc
    14101 atgatggcat ctacttcatt tggaaaagct aaacgaattt ccatcttttt tcctcatttc
    14161 tgactagttt ctcccatcat agcataattt aagtctttct acaagcagtt aaaacttgac
    14221 tttgtatttt tgttatttta taatctagtt atcaaaacta tataaaaagg agttgaaaat
    14281 gaaaactacc ttttcctacc caaaatgggc agaaattcca aacattaacc tctatctgga
    14341 ccaggttttg ctctatgtca atcaggtctg cgcccctatc tctcctaata aagacaaagg
    14401 cctaacagca tctatggtca acaattatgt gaaaaatggt tacctgacaa agcctgacaa
    14461 aaaaaaatac caacgccaac agattgcccg tttgattgct atcacaaccc tcaagtctgt
    14521 attttctatt caagaaatag cccagacact taatactcta caaactcaag caagttcaga
    14581 ccaactctac gatgcttttg tggactacat gaaccaagga attgacccag ctaaccctat
    14641 tatccaaacc agctgccaaa ccattaaact ctatcatcaa actctagact taatcgatca
    14701 tactcaagag gaggtaatcc aatgaacact agtcttaaac tcagcaaaca actcagtttt
    14761 ggagaggaga ttgctaatag cgtgacccat gctgtgggtg cagtcatcat gcttatcttg
    14821 ctgcctattt catccatcta tagttatgaa gcacacggat ttttatcctc tatcggcgtt
    14881 tccattttcg tcatcagtct ctttctcatg ttcctatcat ccaccattta tcactctatg
    14941 gcctatggtt cgacccacaa atatgttttg cgaatcattg accattctat gatttacgtt
    15001 gccattgccg gctcatacac gcccgttgtc ttgaccttga tgaataactg gtttggctat
    15061 ctgattattg tcatccaatg gggaacgacc atctttggta ttctctataa aatctttgct
    15121 aaaaaggtca atgagaaatt tagccttgct ctttacctga ttatgggctg gttggttctg
    15181 gctatcattc ctgccattat cagtcaaacg acacccgttt tttggagtct catggtaact
    15241 ggcggactct gttatacagt tggagctgga ttttatgcca agaaaaaacc ttatttccac
    15301 atgatttggc atctctttat cctagctgcg tccgtactcc aatacatcgc tattgtttat
    15361 tacatgtaaa aaagttgaga aattcaatct caactttttt ctttacacat attgataaag
    15421 tactggtgca agcgcacatc atcagtcaat tctggatgaa aagaacttac caacatattt
    15481 ttttcttggg ctgcaacaat ttgattgttc actgttgcta aaatttctac accctcacca
    15541 acactactga taatcggacc acggataaag gtcattggaa tcttgccaac tcccttacat
    15601 tctgcttccg tgtagaaact tcctaattgg cgcccataag cattacgctc gaccaccata
    15661 tccatagttc ctagatgact ctctttctga gaagtgattt ccttagccag caaaattaac
    15721 cccgcacagg tcccaaacac tggtaagcca gatagaatgg cttctcggat gggaagtagc
    15781 atgttctggt cacgtaagag cttgcccatg gttgtagact caccaccagg caaaatcaaa
    15841 cccgacaagt cactctgatc ttgctgaaaa tcatctagat ttctgagttc tacactctcg
    15901 acacctaatt gatctagcac ttttgcatgt tctgcaaagg ccccttgcaa ggccaatatt
    15961 ccgattttca tctattttcc tcgttcagcc atgagaattt ggatttcatt ttcattaata
    16021 ccaaccatgg cttctcctaa atcttcagag atttgagcta ggatttgagg attacggaag
    16081 ttagtcacag ccttaacaat ggcactcgct cgtttaacag gatctcctga cttgaaaata
    16141 cctgaaccga caaagacccc ctctgcccct aattgcatca ttaacgcagc atctgctggc
    16201 gttgcaacac ctccagcagc gaaatttaca actggcaatt ttccatgttc atgaacatat
    16261 tggaccaatt ctacagggac ttgcaaatcc ttggcagcaa cataaagctc gtcctcacgt
    16321 aagttttgaa tgcggcgaat ttcctgattc atcatacgca tatgacgaac agcttggacg
    16381 atatcccctg tccctggttc tcctttggta cgaatcatgg aagcaccttc agcgatacga
    16441 cgcaaggctt cacccaaatc cttagcacca cagacaaaag gaacttggaa ttctttcttg
    16501 tccacatgga aacggtcatc agctggagat agaacttcac tctcgtcgat ataatcaatt
    16561 tcaatagcct ctaaaatctg agcttcaaca aaatgcccga ttctgacctt agccattact
    16621 ggaatactaa ccgcttcttg gatttcctta atcatctttg ggtcgctcat gcgggaaact
    16681 cctccagctg cacgaatatc agccggaatt cgttccaagg ccatcacagc tgccgcacca
    16741 gcagcttctg cgatacgagc ctgttcagga ttctgcacat ccataataac accacccttg
    16801 agcatctgtg ccaagttttt atttagttca taacgatttt cagtcatttg ttttatcctc
    16861 atcatttgta ttggtagcta ccttttcatt ttaagtcaga acagaatgaa gcacaagata
    16921 aaaaaactat aattgtatgg ggacagatta gctaaaaact atctgacctt aacttaaggc
    16981 cagatagctc attcattttt atttttcagc cgtaagggca gccattgtga tgtagttgta
    17041 tggtttgttg aagtgtggca agaagaagag gtctgtcaat gccaatttat caattgtcac
    17101 atgctcttgg atagcaagtg agaacatgtg gattcccatg ctaattgcaa tatcatgtga
    17161 aaccatttgg gcaccaagaa tttcacggct atctttgtca aagacaatct taattgctac
    17221 ttcatggttg tcatgtttca tgaattctgg tttttgaaga tcgttaaagc ctgtttcagt
    17281 tgcgttgtaa ccagcagctt tcgctttttc aagagtcaaa ccagttgaaa ccatgtgaag
    17341 accgtagatt gagataccat ttgaaccttg aacaccgatt ccttccaatt catgtccaca
    17401 agcgttgtag gcaccaacga taccagtgcg aacagcattt gaagcaagag cgatatagct
    17461 tgtatcttta cgagcattgt cataaacagt cgcacagtca ccaacagcgt aaacgcctgg
    17521 gatagatgtt tcttgtttct tgtctacaag gaaggcaccg ttgcggaaga gttcgatctt
    17581 accatctgca agggctgtgt ttggacggaa accaactgca aggataacca tatccacgtc
    17641 aaagctttct ttgtcagtaa tcaagcgttc aactttaccg tcaccttcga ttgctttaac
    17701 agtttgacct agagccaagc ggatgttgtg atcttccaag ttcttcgcca tcatttgtgt
    17761 gaagtctttg tcatagtaac cgttcaagac agtatcaacg atatcaacaa ggacaacttc
    17821 ttttccaaga cgttcaaagg cttcagcaag ttcaacaccg atgtaaccac caccaacaac
    17881 ggcgatacgg tcgaggtgtt ggctcttgtc agaaagttta ttgataactt cttcagcatt
    17941 ttggtacaat ttcacgaatt gtacgttttc aagagttgct ttaaattcgc ggtttccttt
    18001 aacaatttca acaccttcga ttggtggcaa gattggtgta gaacctgtag cgaaaatcaa
    18061 tttttcgtat gattctttgt gctcttttcc ttcaacttcc gctgtaacta ctttgttatc
    18121 atagtcgatt gaaagaacag gtgagttcat gtaaacttta gcacctttag cttccaattt
    18181 ttctttatca gaatagaaca agccttcagc accgtcaatt tgttcaccaa tccaaagagc
    18241 cattccacat cctaggaaag agatgttaga gttttggtca aatacaacaa tttcgttctc
    18301 atttccaaaa ttatccaaca tggtattgat acatgctgta ccagcgtggt tagcaccgac
    18361 tacaacgatt ttactcataa aaatttcctg ccttcattct aaatttacct tactagtata
    18421 ccatttaccg ttagcgttta caaggggggt actattattt cagcattttt tatcaaaaat
    18481 aattccccat ttatcctgtt ttcaagcctc atgaatttta tgagcatatt tcttgacttt
    18541 ttccaaattt tgtttgtgaa aattctaact ttatggtatt ataataacat gaaaatgaaa
    18601 tgtaagggct tcaatttaga tgattatttg atattacaac ttattttcta taagagaaag
    18661 gagttggtag cttgactctt cgttcacatt ctgaacggct aatggggact actatcacta
    18721 tttcattagt ggatgagcaa gccgatatct ttctccaaaa atcctttgac ttgctcaaag
    18781 aacttgaata ccgcttcaac gccaatagtc aagaatctga gttgatggaa atcaattatc
    18841 aagcaggaat agcccccgtc acggttcatc cagacctgtt tgagctgatt tcacttgggt
    18901 tagagcatag cctagcgctc tctagccacc tcaatatcag cattggtccc ttaattcaaa
    18961 cctggcgtat cggtttttca gatgccaagg tcgcccagcc tcaagaaatt gaatcggtgc
    19021 tgcctctaat caatcctcat ggtatcgagt tagattcttc tacttctact gtgtttttaa
    19081 aacagaaagg aatgaagatc gatctaggtt gtttagccaa aggatacagt gcggataagg
    19141 ttgcccaatt tcttaggaaa gagggggtga cttctgcctt gatcaatctg ggagggaata
    19201 tcctgaccat tggaaaaaat caggcaagag gggataaccc atggcaaatc gggattcaag
    19261 acccagccaa tcctagggga aatcatttaa tgaccatccc tgttgtcaat aaatctgtcg
    19321 tgacttcagg catttatgaa cgtcacctga ccgtcgatgg acaagattac catcacattt
    19381 ttgacagtca aacaggatat cctgttgaaa cggaactagc gagtctaaca atcatctctg
    19441 ataaatcagt cgatggcgaa atctggacga ctcgactctt tggagaaaga cctgcttcta
    19501 tcctctggca agtcgaaaat ttggagggca tcgaagtcat cctcatcgat aaagaaggcc
    19561 acctaagctg ttcttcagga attcctactc tatagagaca actgtttcaa tggaatttta
    19621 aaattgtatt atgtaaaaag agaaaggaaa tctcatgtta aaacttattg ctattgttgg
    19681 aacaaattca aaacgttcta caaaccgtca attgcttcaa tacatgcaaa aacactttgc
    19741 tgacaaagct gaaattgagc ttgttgaaat caaggccatt cctgtcttca acaaaccagc
    19801 tgacaagcaa gtacctgctg aaatattgga aattgctgct aaaatcgaag agacagatgg
    19861 cgttattatc ggtactcctg agtatgatca ctctattcca gctgttttga tgagcgctct
    19921 tgcttggttg tcttatggta tttacccact tttgaacaaa ccaatcatga ttacaggtgc
    19981 ttcttacggt acgcttggtt catctcgtgc ccaattgcaa cttcgtcaaa tcttgaatgc
    20041 tcctgaaatc aaggcaaatg ttcttccaga tgaattcttg ctctcacact ctcttcaagc
    20101 atttaaccca agtggcgact tggttgacct tgatgttatc aagaaattgg atgccatctt
    20161 tgatgacttc cgtatctttg taaaaatcac agaaaaatta cgtaatgcac aagaattact
    20221 tcgcaaagat gctgaagact ttgactggga aaatttgtaa gataggagac caaaaagaat
    20281 gaaatttgtt ggacttgttg gatcaaacta cgatcaatca tataaccgta agctcttgga
    20341 atttatccgt cgctacttca aactcaaatt tgaattagaa gttcttgaaa ttgacgaagt
    20401 tccaatgttt aaccaagatg aaaaatggga tgaaagtttc caattacgtt atttatataa
    20461 caaaattact cgtgcagatg gtgttatcat tgctactcct gagcataacc acactatctc
    20521 agcttcactc aaatctgtac ttgaatggct ttcatacgaa gttcatccat ttgaaaacaa
    20581 gcctgttatg attgtgggtg catcatacta tgaccaagga acatcacgtg ctcaagttca
    20641 ccttcgtaaa atccttgatg ctccaggtgt taatgcctac acgcttccag gtaacgaatt
    20701 ccttcttggt aaagctaagg aagcttttga taacaacgga aatatcacca acgaaggcac
    20761 tgttaaattc cttgaaactt gcttagataa ctttgttaaa tacgtaggag tcgtttcgaa
    20821 attgaaaaaa ccaaaaccaa tcgaatcaga agacttggat tgtggaaaac caatcgctac
    20881 aaccatcaca gaagttgacc ctgacgatcc agaatgggta gaaaaagttg cagcaatcac
    20941 tggtgctgtt tctggtgata cctatgtcaa attggaccac ggtatcctaa cagttaacca
    21001 aattgatatg ttcttgaaag ctatgccatt tgaattgaca tacgctgacg ataacaacca
    21061 attcctctac tacaacaacg ctcaccaaga tccagacact atgttcgcta aacgtgtacc
    21121 acctcaatca ggtagccgta tgtcgactgt tcatggttct cttccaccag cacgtatgaa
    21181 gaacgtagag tgggttatcg gaacacttcg caacggaaac caagaatacg tccgtacgat
    21241 cgttccaggt tctcctgcag gtgttatcaa cactcacaac taccaagcaa tgtactatcc
    21301 tgatggatca tacgctggta tcaatgaaat cgtctttaac ttccaaccat ggcttgactg
    21361 gtacctaaaa gagactggtc aacgtttggt gggtggtagc ggaccatttg ctcctgctgc
    21421 cggaggtcat ggagacgccg atgctacttc tggcgcttct gattcagggg atgctggagg
    21481 ccacggtggt gacgcagacg ctacagccgg tgcaagttac taatgatagt ttttaggaac
    21541 cattatggtt ccttttttag cgaaaaaaga ctagcaaaca caaactgcta gtctctttgt
    21601 tatcgtttca tctatctttg ttaaaagaaa gaatacatat tatgaatttg gatcatcaag
    21661 actacggcca tgtaatcctt tttcacgttg tacttggcgt agtttttctg gtgtaacatc
    21721 gtttccttct tcgtccacaa ttttgattcc ttcaatgtgg tgacgaacag cgcggcgata
    21781 accttcgatg tactcctcac gtagtttggc ttgttccact ttttcttctg gtgttaagcc
    21841 ttctgttttt ttctttttag caagctcatt gatacgagca atttttttcg gatccatttc
    21901 ttctcctctg taataagata aagtccgttt atcaaatttt atttagtgtt aatttggtta
    21961 aaacaagcaa acttagctgc tttcttggtt agttgtgaca agattgccaa acgattttga
    22021 cggacagcct gatcttcagc cattaccata gtattttcaa agaaagcatc aatgactggg
    22081 ctaagcgcaa agagttgttt caattgctga cttgcaggtc ctgataaaac gagtgtttct
    22141 actgcttctg ccaaagcttt ttcttggtca ttctcaaata gtgctgaatc aaccgtagca
    22201 accccttctg ccttctcagc caggttaaag gcacgagaaa gtgattcaac agatggttta
    22261 aaatcttctt ccttgcttac ttctacgaga gcacttgctg cttccaacat atctgccaca
    22321 acaaagtttg aacctgcaag aactgcttct ttgatatctt ttggagtaga gcccatcatc
    22381 ttatcaacac gagccttgat aaagtccata acctctgctt tattttcata agtcaaactg
    22441 tcaaatttca atgcataaag gctatcaatc agctcatcca tagcaatgtg ccaaccaaag
    22501 gcatccaaga tacgaaccac accttgagtt gcacgacgaa gggcataagg gtcattagaa
    22561 cctgatggaa tcaatcctac tgagaagaaa ctcaaaatcg tatccaattt gtctgcaatg
    22621 gctagaactg cgccgacctt gctctctgga agttctcctt cagctgatgt aggcatgtag
    22681 tgttcacgaa tagcagctgc caccgctgga gtttcaccag caagaagggt gtatttttca
    22741 cccataattc cttggagttc gtcaaattca ccaaccatac ctgtcaacaa gtcaaacttg
    22801 taaatggctg ctgcacgagc aaggtcaact gtttcatcca ctgacaaacc agctttttct
    22861 gccaaaagta cagtgatttg acccgtacga atcatgtgtt cacgaaggga accaatcttc
    22921 tcatggaagg tgacattgtt taatttttca acaagatctg aaatcaccaa tttttggtct
    22981 tcacgccaga agaattctcc gtcttccaag cgggctacca agactttttc atttcctttg
    23041 atgacatttt tcaaacgctc tgcgtttccg ttacgaacag aaatgaagtt tggcaagagt
    23101 tttccatctt gatcacgaac aacaaagtaa cgctggtgtt ccttcataga agtcaccaag
    23161 acttcttctg gaacttcaag gtatttagca tcaaaacttc ccatgaaggc agttgggtat
    23221 tcaaccaaat tcaagacttc attcagcaaa tccgcatcaa tttcgatacg tacaccatgt
    23281 tttgcctcaa tttccttgat ttggtcaaca atcatttgct cacgttcaca tggatctgcg
    23341 attacaaact gcttacgaag gtcttcttca tagctcaatg ctgactgaat tttggtttct
    23401 ttccccaaga aacgatggcc acggctcaca cgacttccct tgatatcaag gaaatccaag
    23461 tcaaactctt gctcatccaa gagaacagtt aaagtgtgga cagggcggat gtattcaaag
    23521 ctatttccag cccagtgcat gctgacaggg aaagtcaatg acttcaagac atctacaaca
    23581 ccaggaacaa tggcttcaac tgcttgacca acttcttcct tagtgacata gacatattct
    23641 tcacccttga tttcacggaa ttcgatatct tcaacagtca agccttttcc acggacaaat
    23701 ccttgagctg ctttggtgaa gtttccatca ctatccaagg caattttctt tgctggtccc
    23761 ttgaaatctt ctgtcaaatc agactgtttg tctgataaac cagtcacacg aacagccaaa
    23821 cgacgtggtg ttgagaaggt ttgaatggct tcaaaagaca ggcggttttc cttgaggaag
    23881 gctgccattt tttcgcctag ttgtttttca cttggtgtga caacataggc tggtaattct
    23941 tcaagaccga gttctactaa taagtttttt gtcatgtttt ttcctttaca ttttcttcaa
    24001 tcttttttga tatgcacctt aggtactggg gacgtcgcta actaactctg tgagtctgta
    24061 tggaaatcca atgctaaatt tatcaagatt tccaacaatc tcatcaaagt ggatttagct
    24121 gactgatttt tgaacctaag gtttcaaaaa tccccacata acagtacggc ggtgcatatc
    24181 cctctagcaa gtcttcgact tgcctctaac gattttagag tacagtatta ctcactattc
    24241 tgcgtcttct gctaaaagtt tagctcttgt ttcttcatcc aaaagtgggt agcctaggcg
    24301 tttgcgttct gcgacaaagg ttttggctac gacacgggcc aagttacgga tacgagcgat
    24361 atagcctgca cgctctgtta cagatacggc accacgcgcg tcaagcagat taaaggtatg
    24421 tgaacatttg agaacatagt cataggcagg gtgtaccaag ccttcttcta atgcacgacc
    24481 agcttctttt tcaaacttat caaagttttc aagcaacatt tcttggtccg aaatttcaaa
    24541 tgaatatttt gagtgctcat actcaggctg gataaagatt tctccgtatt ttacaccatc
    24601 agcccactcg atatcataga cagagtctac ttcttgaatg taagaagcca agcgctccaa
    24661 accataggta acttccgcag tcacagggct agttgccaat ccaccgactt gttggaaata
    24721 agtgaactga gtgatttcca ttccgtcaag ccaaacttcc caaccaagac cagctgaacc
    24781 agttgatggg ttttcccagt tgtcctcaac aaaacgaata tcgtgctcca aaggattgat
    24841 tcccaatttt tccaaagact caaggtaaag ttcttggata tttgatggag aaggcttcat
    24901 gaccacctgg aattggtggt gttggtagag acggttaggg ttttccccat aacgaccgtc
    24961 agcaggacga cgtgatggct ctacataagc tgcattccat ggctcaggtc cgatagcacg
    25021 aaggaaagtg taaggactca ttgttcccgc acctttttca ttatcataag cctgcataag
    25081 catacaacct tggtcattcc aaaattgttg caaagtcaaa ataatttctt gaaatgttaa
    25141 tttcttagac attgaatact ccttaataaa aaataattac ttgcgacacg agtctgcctc
    25201 gtcgatcata cgaggcaaga cgagttagta ctgcgtctag ctcagcaccc tagtgctgag
    25261 cgtggagcag tccgactgta aggaggtttc tgccactgac gcagtgaaat gtcaatttct
    25321 tagacattgt ttactccttt agtttttata tttctttttc aattaatttc tgctgaacaa
    25381 accaatctaa caagggaaaa gttttttctg tatttgccca taaatgataa gaatctaaga
    25441 ccgcttgtac actacctaaa ttttctacca acttatctac tgtcaaaaag ctgcgccagt
    25501 attcataaaa aatacttgca tagttcttat cataagtcga agaaccaaaa tcattcaatg
    25561 aatgccaacc atacttcttc tgaaaaagtt ctacgagaga ttgattacaa attttttccg
    25621 cttgaaattc ctcttctgtc aagaaatact tgcgactaat atattcaacc atcccctctt
    25681 cgaaccagat ataagagtca taaccatcaa aatcatctaa aaaatgctcc gaccaatgag
    25741 ctaactcatg tcctacaatc tgtaagagga aattttcaga taaagaatga tagtgacttt
    25801 ctattgcttg agtttgctga gaacactcgt agtccatcaa ttgatacaaa tacaactctt
    25861 tccaaacagc taaatcaggc gccataacca ttcgtctatt atttgtatag gctggaacag
    25921 cgctttccct aataatctgt gtagcagcat taaaatctga ccaaataacg gcttgcggta
    25981 aatcacagac tgcaagttca ttctttaaaa aagattgata atctttcaac ttttcagtat
    26041 ttcttactac aaaatcacga aatgtagcta gttgactatc gtcttttaca agataaaggt
    26101 tgtccacggc tctcctttct ttcacacaat agactgaata agcaaaagaa ccgcgtccga
    26161 caatcctagg ttgatttaac ctaggggcgt cagaaacgcg gttccaccct aatttattac
    26221 ttcattgttt ttgaaaatac tacagaaagc gccagctcat tattttgccc tacttggctc
    26281 ccactatccc aagctcgctt gaagaacgcc ataagacttt ctttcctgct gactattata
    26341 tcaaaaatta gaataatgta caattctttt tatgattttc tagaaatcca catcatcaac
    26401 tcgtggagca cccgattgaa cagcaatcgt ttttaaggtt tctctctcct catgactcag
    26461 ttcaattcca aagcaatcaa gattagcttg aatacgagag gttgtgacag attttggaag
    26521 tggtaaaaat ccttctgcca agctccaggc caaggctatc tgagcaatcg attttccgtg
    26581 atttgctgcg atttcttgca cttgcttgct atcaaatagt tctccctgac caaaaggccc
    26641 ccaagcttcc aataaaattc ccttttcacg acagtaagct acgacttgat cttgatacac
    26701 acctggcgcc aagcgaactt gattgaccgc cggaagaatt tttgctgttt caagcaaggc
    26761 atccaaatga tggggaagaa aattactaac accgatagca cggattttcc cttcttgata
    26821 gaggtcttcc atcgctctcc aaacttctgc atttcggatt ttccattggt cattttctct
    26881 gagcggtttt ggatttggcc aatgaatcaa atacagatcc aaataatcca agcccagttt
    26941 ctctaaagac tcttcaaaag cctgacgagc ttgctcatag gtttgttggc tattccacag
    27001 cttggtagtt acgaacaatt cctctcgtgg aactccgcta tctttgattg cttgaccaac
    27061 actttcttca ttctgataaa tcgccgccgt atcaatatga cgataaccag ccttcaaggc
    27121 ttctaacact gcacgatagg cttcttctcc atccttagcc ttaaaagttc caaatcccaa
    27181 tactggaatt tctactccat tatttaattg ataggtatcc atatgcttct ccttctattt
    27241 tatactctct tcaaaccacg tcaacgtcgc cttgccgtag gtattgttac tgacttcgtc
    27301 agttctatcc acaacctcaa aacactgttt tgagcaacct gcagctagtt tcctagtttg
    27361 ctctttgatt ttcattgagt attattcatc aattatagca taaaacattt tttaagtagt
    27421 actatctttt gtaaaaaata agtctatcac atatctgaca gacctatttt ttcttgctta
    27481 ttcatcacga ctataataca gctcatgagc ttttaggcga gtattgagca tctctggtat
    27541 ggtcacaaag gtataacctt gatttttcaa atactcaatg acccttggca aggcattgac
    27601 tgtcggactg tgaatatcat gcatcaaaac gatagagccg ttagctactt gatgctgaat
    27661 ttctgtcaaa atagatgctt catttttact cttccagtcc agactatcca catcccacat
    27721 gataaagctc aaatccaagc tattgcgaat atcatctgta atagcaccat aaggtggacg
    27781 catgagttta gaactagaac ccagcacttt agttagcaca tcctcagtat cagtaatctg
    27841 ctttttagct tcatcaagag agagttgcga gagaatcgga tggctccaac tatggtttcc
    27901 aacaacatga ccttcagatt ttatcctctt caccaagtcc tcattcccag aaacattttt
    27961 cccaagcaca aagaaagtcg ctttaatatc atatttagct agggtctcta atacctgcgg
    28021 ggtcgttgct ggatttggac catcatcaaa ggttagagcg acaacttttt gatgtttctt
    28081 atcaaagtaa gattggtaca aggccgcatc tttttcgagt aagtacgaag attggataac
    28141 atcaaagaaa gcagatactg gcaaggctat ctcttctaaa ttttcaacca caggacttgg
    28201 ataaaggata atctgactat ccttgtaatc aaaattccat gcagacaagt cttggtcaga
    28261 gaagtttttt acaatctgct cactctggtc ttgctctatt tttttatcct ctatgaagga
    28321 ggtcaactct tttatcagct ggtccttagc cttactagca tctgaaaaca gttggtcaag
    28381 tgtaaaaggt tgcccatctt ctgttaagtg aacctttcct agactggtct tttcagtctc
    28441 ttcaatctta aaagcaacta aatcatagat ttgtttggtc acattacgat taacgactcc
    28501 ctttaaacct gactcttctt gctctgtata gtagaaaaca agattgtcct tactttctag
    28561 cttgtcctta acatcttgat ttatcagctc cctaacagag gaaatcactt tctccccttg
    28621 gagaggataa taggcaatca cttcggcttg cccctgacga aaatgctcct tctgatttcc
    28681 ctcactcaat tgatcatctt tctctttttt gagcgattca atcttttgct caaaactttt
    28741 ttgctggtag atcttaaaag caataaatcc gcctaatagg caaatagaaa ttgctaaaat
    28801 accaatcaaa gccaataata catgtctcgt tttcccgtgt ctgccacgtc ctagtctact
    28861 tttattcata tttatatcat aacaaatttg agcaataatt tcaatgatac aatttggcaa
    28921 tccacaattc ctctagcaag ccaaagcatc ttacatcaac tccaacatcc gtatctgtag
    28981 agattatgta gttttctatc tatggaattt ctagtagatt gaagctagaa tagtagacct
    29041 atacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctc
    29101 atttcatttt actataaaac tatgaaaagc taggtcatct gagaagtaat tactatcgac
    29161 aattgcgata gcgtatacta gtgcgatttg tttgtagctc tggtgggtat caccttcacg
    29221 ttggcgaatt ttttctaggt tttgattaac ctaactacac agataagccc ataggcgatt
    29281 gatgaaaaaa ttctgatgat gaacctgaat ttcgatgata acttggattc cattatctaa
    29341 ctccgccaag acgtctatac tagtatagaa atcctgcgct gagtagggca tggaaggcaa
    29401 gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt ccagcatatc
    29461 gcggataaat tgacaaatca ttagttgggc tgatacccgg atgtctgaga atcctccttt
    29521 tcctccttct attatagcac atttttaaat ctaggtttgc tagattctat gcttctatct
    29581 atttattcgg aaaagaaagc ttttcttcag aagaaagcta atagcttatt tgaaattttc
    29641 tcctctctat tggatagtcc cgtctatcta tgttataatg aaagaaggat ttaaaatcgg
    29701 aaaaggagta tttatgctaa aattaggtgt catcggaact ggcgctatca gccatcattt
    29761 catagaagca gcccatacca gtggagaata ccaactggtc gcaatctatt ctagaaaact
    29821 agaaactgca gcaacctttg cttctcgcta tcagaatatc caactattcg atcaattaga
    29881 agtcttcttc aaatcttctt ttgatttagt ctatatcgcc agtccaaact ccttacattt
    29941 tgctcaggca aaagctgcct tgtctgctgg taaacatgtt attcttgaaa agccagctgt
    30001 cactcaacca caagaatggt ttgatttgat tcaaacagct gaaaaaaata actgttttat
    30061 ctttgaagca gctcgtaatt atcacgagaa agcctttact actatcaaaa actttttagc
    30121 agataagcaa gtgttaggag cagatttcaa ctatgccaaa tactcttcca agatgcctga
    30181 cttattggct ggacaaacac caaatgtctt ttcagaccgt tttgctggtg gagccctcat
    30241 ggatttgggg atttatcccc tctatgctgc tgttcgtctt tttgaaaaag ctaatgacgc
    30301 gacttaccat gctcaacagc ttgacaatag cattgaccta aatggagatg gtatcctctt
    30361 ctacccagac tatcaagttc acatcaaggc tggaaaaaac atcacttcca atcttccttg
    30421 cgagatttat acaacagatg gaaccttgac tctcaacacg attgagcata ttcgctcagc
    30481 tatttttacc gaccaccaag gaaatcaagt ccagcttcct atccaacagg ctcctcatac
    30541 gatgactgag gaagtcgctg catttgcaca catgatccag caaccagatc tgaatctcta
    30601 ccaaacttgg ctgtatgatg caggctctgt tcatgagcta ctatatacca tgcgccagac
    30661 tgctggcgtt agatttgagg cagaaaaatg aaaaccaaac taccgactga atggcaagaa
    30721 ctgagtgacc aactcggttt ccaagaattc acccccattc aaactcaact atttgagctc
    30781 cttcttgctg gagaaaacct cctaggagtg agccaaaccg gaactggtaa gaccctagcg
    30841 tatctcctac caagtcttct cagactacaa aagaaaaaag cccaacaact cttgattcta
    30901 gcaccaaaca cggaacttgc tggacagatt tttgatgtat gtaaaacgtg ggcagaagct
    30961 atcggattga ctgctcaact cttcctttca ggttcaagtc agaaacgaca aattgaacgt
    31021 cttaaaaagg ggccagaaat tctgattgga actcctggcc gtatctttga actcattaaa
    31081 cttaaaaaaa tcaagatgat gaatgtggaa accatcatcc tggatgaatt tgaccaatta
    31141 ctagatgatt ctcagattca ctttgtagag aaaatcactc actacgcacc tcgtgaccac
    31201 caactggtct acatgagtgc cacgaccaag tttgaccaag aaaagattgt gccgaataca
    31261 cgcactattg atctctctga tcaaaaattg gacaatatcc agcatttcta catgcaggta
    31321 gaccaacgtc atcgagtgga tatgctacga aaactggctc atgtagagga tttccgtggt
    31381 ctagtcttct tcaatagcct gtcagacctt gggaatgccg aggaaaaact acagtatcgt
    31441 gatatattgg ctgtttccct cgctagtgat gttaatgtta aatttagaaa aatcatctta
    31501 gaaaagttta aagataacca actaaccctg cttcttgcaa ctgacctttt ggctcgtgga
    31561 attgatatcg atagcctaga atgtgtcgta aactttgata ttcctagaga tagcgaaacc
    31621 tacacacacc gtgctggccg tactggtcgt atgggcaagg aaggttatgt tatcactctc
    31681 gtcactcatc cagaagaaat caaaaaactt aaaaagtttg caagtatccg agaaattgtc
    31741 ctaaaaaacc aagaactcta tatcaaataa agccccattg ctctatacac atccagtgta
    31801 tggagctttt ttataagcaa ttcaatttta tactcatctg ctttcaaaaa gcattctagt
    31861 ccatctccga ttaacgatgg actttatcac ctccttctcc agtccttgta tgacatcttg
    31921 aagttgattc atgacatctt ccaaagttcg aaaggcctta ttcttaaatc cacgtttacg
    31981 aatctctttc cacacttgtt caatgggttc atctctggtg tgtatggagg aataaaggta
    32041 aaaccaatat tagtcggaat ctttaaggta cttgatttat gccatatagc attgtccata
    32101 acgagtaaaa gataatcatc tggataagct tgtgaaagct cttctaaaaa ggcgttcatc
    32161 cactcagtat tacatccacc agctattaag aaaaatgatt cgcctgtatg ggcatcaaca
    32221 gctccataac aatagtgaaa ttctcgtata tagtgactat ggacatgtgg acctactcct
    32281 attggagccc aacaagatcc cagtttactg attctaccga aaccagcctc atcttggtac
    32341 atcaagcgaa ccttacgaaa acgtctactg cttttaaagc gctttctttt cttcttgaat
    32401 tgagatttta tttttagacg cgacaatggt ttgagcgtct gctttcctag gatgttctgg
    32461 acgtggcata atatttcgcc aaccatggtg cttcaacagt tgatagaagg catcacgtgt
    32521 gtaggaacga cctaactcct ttttataagc ctgaaataag gcatcaattg taacaaattc
    32581 tcctgcctct gtagccttca aatggcgggc aagaaagact ttctcttcct caactgtcat
    32641 atatgcatgg ttacggccac cacgtgtttc ttgaaggaga gagtcgagtt cgaactcctc
    32701 atattttttt acatttcgcc aaatcattgt ttgattacag tctaaaagtt ctataatctc
    32761 tttataagat ttgcccatat cagacgaaat agaacgattt gaagacgttt atgatattta
    32821 gctgtacgag agtcttttaa aagtgttttg atggtttgga tttcttcttt aattgatttc
    32881 atattactat tgtataatgc tttttgattt tagtctagta ttatactctt cgaaaatctc
    32941 ttcaaaccgc gtcagcttcg ccttgtcgta tagatgttac tgacttcgtc agttctatct
    33001 acaacctaaa aacagtgttt tgagcagtct gtagctaact ttctatagta aaatgaaata
    33061 agaacaagac aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaaa
    33121 tgtgtactat tctagtttca atatattata gtttgctctt tgattttcat tgagtattag
    33181 ttcaatatct aaaaaaagaa ccttgccaag caagattctt ttagtgtctg acttaaataa
    33241 ttgttcttct gggaactaaa tcgaattaag cttcgatttc tgtaaccata cctgaaccaa
    33301 cagtacgtcc accctcacgg atagagaatg tagtaccttg ttctacggcg attgggtgaa
    33361 tcaactcaac gtcgattgtc acgttatcac caggcattac catttcagta cctgctggaa
    33421 gttcgattga acctgtaacg tcagtagtac ggaagtagaa ttgtggacgg tagttgttga
    33481 agaatggagt gtgacgtcca ccttcttctt tagtaaggat gtagacttca cctttgaatt
    33541 tagtgtgtgg gttgattgaa cctggtttag cgataacttg tccacgttcg atttcatcac
    33601 gttgaacacc acgaagaagg acacctacgt tatctccagc aagaccttcg tcaagttgtt
    33661 tacggaacat ttcaacacca gtaacaactg ctttttgagt ttcttctttg ataccaacga
    33721 tttcgatttc gtcgttgact ttaacgatac cacggtcgat acgtcctgaa gcaactgtac
    33781 cacgtccagt gattgagaat acgtcctcga ctggaagaag caatggtttg tcagtgtcac
    33841 gttctggttc tgggatatac tcatcaactg tgttcatcaa ttccataacg atgtcttcgt
    33901 atttagagtc accttcaaga gctttaagtg ctgaaccttg gataactgga agatcgtcac
    33961 ctgggaagtc gtattctgac aataggtcac ggatttccat ttcaaccaat tcaagcaatt
    34021 cttcgtcgtc aaccaagtca actttgttca tgaagacgat aaggtgttta acaccaacct
    34081 gacgtgaaag aaggatgtgc tcacgagttt gtggcattgg tccgtcagtt gaagctacta
    34141 caaggatagc tccgtccatt tgagcagcac cagtgatcat gtttttaacg tagtccgcgt
    34201 gtcctggagc gtcgatgtga gcgtagtgac gtttttcagt ttcgtactca acgtgcgcag
    34261 tgttgatagt gataccgcgt tcgcgttctt ctggagcagc atcgatagac gcatagtctt
    34321 taggttggtt aactgatgaa ggcaagcgac gtgccaaaac agttgtgata gctgcagtta
    34381 gggtagtttt accgtggtca acgtgtccga tagtaccaat gttaacgtgt ggtttactac
    34441 gatcgtattt ttcttttgcc atttgagtaa aagcctccaa taaaatatat tttatagata
    34501 gacagtaggc aatacagtct aactttcctt actattttat caaatttcag ctaaattgca
    34561 agtgttttac aacgtttttg tgaattattc ttgataaaaa tttaactaac taatagaaag
    34621 tactaaatag acttatcctt caaggagttt tcagcattat ccatccactc acttgctgaa
    34681 aatgtaaaca gtctctggtt tatatagtaa aatgaaacaa gaatagggca aatcgatcag
    34741 gacagtcaaa tcgatttcta acaatgtttt agaagttgag gtgtactatt ctagtttcaa
    34801 tctactatac aacctcaaaa ctgtgttttg aacaacctgc ggctagcttc ttagtttgcc
    34861 ctttgatttt cattgagtat aaaattgcag ataaaacgat aacaatcata ggactcagca
    34921 gagtcaatgt aggtcgaaga atagccctcc taatcgctta ttttatagca aaatgaaata
    34981 aaaactgaac attttgttga ggaaggtaaa tcaattcctc acaatgtttg agaaagaagg
    35041 gaggactatt ccaacttcaa cccactataa agcagactta caagataggg aacccatctc
    35101 cgataatata tagtagaatg aaaactgcac aatttgattg gagaattcaa accaatttct
    35161 aacaatgttt tagaaacaag ggtctattat ttcaactgca acctactata gcccattgaa
    35221 gctggaatag cccactgaga cttctaaacc actgctggag aggagtttgt ttagagtttc
    35281 tttcatttcc ctaaaaaatc aaaaagagag aaccagtctg atcctctctt acttatagat
    35341 ggtgctcact caaatgttaa aggagctatt tcttagagat aaaggaattt gaatacagct
    35401 actgccgcaa ttgctgctgc gataggtgct actactggta cccaagaata ccaccatttt
    35461 gaatcgcctt tatgctcacc aagaactgat tttggaagga aagcatgaag gagacgtggt
    35521 cccaagtcac gggctgggtt caaggcaggt cctgtaggtc ctccaagtga tgttaccaaa
    35581 gccataacga ggaatccaag tgccaagtga gccacagaaa gtcctgaagc agtgtgtgga
    35641 gccacctgtg ctttaatagc caagtcagaa aaatcaactg tttgtcctgc ttctattgcc
    35701 ttttgtttca tgaattgaag cacttcagca ccaaagaagt ttttagtcaa accaagagct
    35761 gcaaagaaca aaacaaatga accaacaaac tcattaatca aaccattgac agttgctgca
    35821 tagcgacttt cttttgtacc atggtcaata cttgaaatag ttgagaaagt tcccaagatg
    35881 ttatttggtt tttcagtttt caagtagaat ggacggtatg ttgccacaac taaggcttgg
    35941 ccaaagatag cccccaagac ttgcgcgata atgtaaggta ccacttgtgc ccaagggaaa
    36001 agaccgctaa ctgcaagccc tagagtgaaa gcagggttga tgtgattccc agatacgtta
    36061 ccaaacatca aggctgggat cataaccccc ataccataac caacagcgat gacgatccag
    36121 ccactttggt gacctttcgt acctttaagt tcaacgttgg caactgcacc attcccaaga
    36181 atgatcaaaa tggcagttcc caaaaattca gtggcatact tcagtgccca tgtgaaatcc
    36241 atttgctaga ttctccttaa aattttttag atattcctta ttttatcaat ttttacccta
    36301 tttagcaact gatttctgga aatattttta aaaaaatagg attcatagtt tcccagttca
    36361 acgtacttgt aagcgttttt attcttcacg aaagacctca ttctcaaaac caggaaaaag
    36421 cccacatctg tgatcttttc cttgttccta gattctaaaa tctattattt ttcatcttct
    36481 ttttcttgaa gaatcctaat cctagaccag ctaacaaggt catgatacca gcactagcca
    36541 aggtagcatt agcttctgta cctgtgtttg gcaattcctc tctcttacct gtctcataag
    36601 tcggaacttc tgggtctgga ttcactggag tttcagtttt tggagtacct gggtctggag
    36661 ttggtttatc tggtgttgat aaacggtcat accttaccgt tatttcttta tcactagagt
    36721 ctgacgtaac ttcttgtgat tcaactgttg gaatatctgg atctttgtac ttgtcaatct
    36781 taccagatac aacctcgtcc cagtttcctg ttgtccattc accgtaggtt acaactcccg
    36841 tgaccttgtt ctcagttttt gtacggctta aggttacagg ttgaacaaca tcttctttta
    36901 cattttggtt cgtaacttta tcaacgtaat gaatgatacg cgttatagtc ttcgtctcag
    36961 tagaggttgc tgttttggga accactgttt cctcaacatt ctcacggtag taatagtcaa
    37021 ctgttgcacc gtcttctggt acgcatttgc aggagttgct accaaggtgt atgttgtttt
    37081 ccttgtgata actcggtctt ctttgtcctc agttgttgtt ttcccttcaa tagtttttga
    37141 ttctgtggta tactcagaac ctatcgctaa atcagctttt ataacagact ctgccaactt
    37201 ctcttggcta ccttctttat agtaattggc tgttactgta gcagtggttg gcgcttcgct
    37261 ttctctataa actaaggtca ctgttctacc ttcgcttaca atattcccag ttaaacttgc
    37321 agaatttgta tctgcttctt taaaagtata atattttccg tcagtagtag tcatgctact
    37381 gagtttttta tctgtgacat tgtagctggt accaatcagt tgttttttat tggtaatgta
    37441 ggttccgtca ctttcttttt ctccaattcc agtatcattt ttcaatgata gcaaacgccc
    37501 ttgttcatca acatagcgaa ctttcacatt ttctgagatt agttctgcca attctgaggt
    37561 ttttttcttt ttcttgattt cttcggttat tttccctttc tcttcttcgg gaatatttag
    37621 ttttggaatg attttttcaa caacggttcg tgatggttcc acagtatctt ggatgactga
    37681 aaagtcagct agaattggga gattatactg aacacggtga ctttgagtgt ttactcctac
    37741 tctttcatta ttctctgaaa atacttgtac ggtataagaa acaacatctt ttcctaatag
    37801 aacatcccca gtagagaaat agccgccttt tcctagtttg ctatctccag agtccacttc
    37861 tttcctaatc ttatcagata gttttttacc agtcagtaca ttcgttcgca caatcccttt
    37921 gtctacccct acaaagtggg agaacttttt gaactcttca gaaccagatc tagcccaacc
    37981 attattaagg gcatttgctt ttgtatttgt attctctctc aaaggtttgg cgattagaat
    38041 tatattcatc ggcacttaga gttgctgcta tatctgactc ttgaatacca acttccttac
    38101 taccatttct agcggcagta tatgtgtatt tatctgtttc tacttctagc tgattatcct
    38161 taacatgata ggtaattttc tcctggtata agagtcttca gtagtttata tgctctgtca
    38221 tcatctttag ctttagaagc agctgagttt agccaagttg gggtattagg agtatcttta
    38281 tcagcctgtt tcaagacatt attcaagtca tttgctaacc tattggttgt cctcactgtg
    38341 aaagttttct tccagtctga atcactttta ttagacttag atatgctcat gaaatcctgg
    38401 tcaacattca acacaatatc gccttgtttt ttagcatctt ccgttgcttc tgcttcaaat
    38461 tgatcatgga gtttttgttg ctccggtgta tagttttttt atttacacga tttaatgtac
    38521 tattttttat tttagtatta agaactccat taatacgatt tttaaattct gcttcaaaaa
    38581 atcccttaat ttgttggtgc gcaagttcaa cattcaaacc tttcttatac aagtcttcat
    38641 tctgatacaa gtcttcattc tgaacgattt cggctacacg gtgaatggtt tgatcgtata
    38701 ccgtcttctc ttaaccagca gtatccttag ttttaatgac tactcgatag gttcctttag
    38761 aaccgttata gtcatctagt tttaaaaaag cattccaaac attcaaaaat agttggaact
    38821 tcccttaata aatccgtcac tttctttttc aagatagccc aagcttgctc aataggattc
    38881 aaatccggtg aataaggtgg tagaggtaag aaaaagtgtt tatcttggat gcagagttca
    38941 tccaaaatgt tcttgggatg aaaactagca ttgcccatga caataacatg aggtgtcttc
    39001 aaagcaggta ggagttgcgt tttgaaccac tccacaaaga aatcgcttgt catgcttttc
    39061 ttgtaaatca tgggagctat aaactctccg tctacttgtc ctgcaacaat tgaagttcgc
    39121 tcaaaacgac gtccgctaat cttttcatag actttctccc ctctaggagc ccctgcataa
    39181 ggacgataga ggtagcggtc gattcctgtt tcatcaatat atacgattgg taaatctttt
    39241 aggttatcca aaatatcaag aaactcagcg actttctctg actcttgttc cttaaagatc
    39301 gtcatctttt tttaaagtga ccttaatctg ctttaaagct gcccatactg aaggaacagc
    39361 acaatcaaaa tgtgccgcaa tttcccgtaa aaaagcatct ggatgagcct ctacaaaggc
    39421 tttcaattct tctaaaggga tcttacgctt tttgacgact cgctttttcc gctctaagtt
    39481 cctttgttca cgtctttctt ttcccacgtg aagagagttc tgacgccaac accaaaaaac
    39541 tttggccgcc tcgacatgtc tgtgccctcc tttgatggaa tctaatgctc cttgtttaaa
    39601 atcagtatta tatgccatag ctttattaga ccatgcttat tttaaactaa gcaactatat
    39661 caatgttttg ttatgagaag acttactttt tttactgaaa ttgaattttt cctagcctca
    39721 acatcatata accccaaaat tcaatctgac ctggtttgaa gagatgttcg aagcagataa
    39781 ggttttacta aaactaaaaa aaatgatata atcaaactga ttaaatgttt taaaaataaa
    39841 ggagaatttg aatgtcttac caagaaaatt accagaaatg ggttgatttt gtggagcttc
    39901 ctgactacct tcgtcaagat ttggaaaata tggacgaaaa aactaaggaa gatgccttct
    39961 atacaaatct tgaatttggt actgcaggta tgcgtggctt ggttggtgct ggtacaaacc
    40021 gtatcaacat ctacgttgtt cgccaagcta ctgaaggatt ggctcgtttg attgagtcaa
    40081 aaggtggaaa cgagaaagaa cgcggtgtag caattgccta cgatagccgt cacttctcac
    40141 ctgagtttgc ctttgaatct gcggcagttc ttgctaaaca cggcatcaaa tcttacgtat
    40201 ttgaaagcct tcgtccgact ccagaactat catttgcagt tcgtcatctc aactgtttcg
    40261 caggtatcat ggtcacagcc agccacaacc ctgctccatt taacggttac aaggtttacg
    40321 gtgaagacgg tggacaaatg cctccacacg atgcggacgc tttgactact tatatccgtg
    40381 caatcgaaaa cccatttgca gttgaagttg ctgatgtgga aactgaaaaa gcttctggct
    40441 tgattgaagt tatcggcgaa gctgttgacg tagaatacct taaagaggtt aaggacgtaa
    40501 acatcaaccc agccttgatt gaagaatttg gtaaagacat gaagattgtc tacacaccac
    40561 ttcatggtac tggtgaaatg ttggctcgtc gtgctcttgc ccaagcagga tttgactctg
    40621 ttcaagttgt tgaagcgcaa gcaactgctg acccagactt ctcaactgta acatctccaa
    40681 acccagaaag ccaagcagcc tttgcccttg ctgaagaact tggtcgtcaa gttggtgcag
    40741 atgttcttgt cgcaactgac ccagacgctg accgtgttgg tgttgaagtt cttcaaaaag
    40801 atggtagcta cctcaacctt tcaggtaacc aaatcggtgc tatcatggct aaatacatct
    40861 tggaagctca caaaaacgct ggaactcttc ctgaaaatgc cgctctctgc aaatctatcg
    40921 tttcaactga cttggtaacg aagattgctg aaagctacgg cgcaactatg ttcaacgtct
    40981 tgacaggttt caaatttatc gctgaaaaaa tccaagaatt cgaagaaaaa cacaaccaca
    41041 cttacatgat gggatttgaa gaaagcttcg gttacttgat taaaccattt gtacgtgata
    41101 aagatgccat ccaagccgtt cttgtcgttg ctgaacttgc tgcctactac cgttctcgtg
    41161 gtttgacact tgctgacggt atcgaagaaa tctataaaga atatggctac tacgcagaaa
    41221 aaacaatctc tgttactctt tcaggtgtcg atggtgctga acaaatcaaa gcgattatgg
    41281 ctaaattccg caacaatgct ccaaaagaat ggaacgcaac agctatcact gtcgtagaag
    41341 acttcaaggc acaaactgct actgttgctg acggtactgt tacaaacttg acaactcctc
    41401 caagtgatgt gttgaaatac acacttgctg acggttcatg gattgccgtt cgcccttcag
    41461 gtacagaacc aaaaatcaag ttctacattg cagttgtagg ggaaaccaac gaagaatcac
    41521 aagctaagat tgctaacatc gaagcagaaa tcaatgcatt tgtaaaataa agacctataa
    41581 aagatgagac caaccaacct catctttttt cgtatcaaat ctaagcaatt ttagaaactt
    41641 tatggcatac tagacttatc aatgaaagcg aggtaatctc atggaacaat ttttagataa
    41701 catcaaagac cttgaagtca ctacagttgt gcgtgcgcaa gaagctcttg ataaaaaaga
    41761 aactgcaacc ttctttatcg gtcgcaaaac ttgcccttac tgccgtaaat ttgcaggtac
    41821 attgtcaggt gtcgtagctg aaaccaaagc tcacatttac ttcatcaata gtgaagaacc
    41881 aagccaactc aatgatttgc aagcattccg ctcacgctat ggaatcccaa ctgtaccagg
    41941 ttttgttcat attacagatg gacaaatcaa cgtccgttgc gactcttcaa tgtcagcaca
    42001 agaaatcaaa gatttcgcag gattgtaaaa aatcagctaa caagtcttaa aatcacaaaa
    42061 acgcataatg taaggtgcaa aaaccttgat attatgcgtt ttattgttcc taaaacagtt
    42121 acatcagcat ttcattacag aacattttaa ttttggaagt caaatcaatt tttaacaatg
    42181 ttttagaaat cacagtgggc tattccatat tcaattatcc agattcaatc gctattatcg
    42241 ttttcctaga ttacaaaaaa actgcgtttt gctaatcaaa acgcagtctc agccactatt
    42301 ttatcttact gtccttcttt tacttctttg gttgctacat cttctccaaa ccatttttgg
    42361 ctgatttctt ggaacttgcc gtccttgtaa agactagaaa aagcttcatt tatcttctta
    42421 accaaggttg tatcttcctt acgggctcca accgcaaaag cttctgtttc tagtccaact
    42481 gtaaagacat tataatcgtt taaaacacct tctgcttcta aataatagtt tgcatagaca
    42541 cggtcaatca atagaccatc aattcgatcg tttttcaaat caatcaaggc ttcattaaag
    42601 gtttggtatt gattcgcttc cttattagcg acaatattct tcaaaatttc tggatttgct
    42661 tcaaagtccg cataaccaga tgaaccagct tgagctccta atgtctttcc agtcatatcc
    42721 tttgcagtcg tgataccaga tgatttcttc gtaaccaata cctgctcatt cttcatatat
    42781 gagttactga aagccacctt ttcacggcgt tcgtctgtag cggaatagcc attccaaatc
    42841 agatcaatcg ttccttttgt caattcagct tctttcaaat cccaatcaat cggttgccaa
    42901 tttaccgtga ttccgtattt ttcaaaaaca gctgtagcta aatcaatatc aaatcctgca
    42961 taagaaccat ctttctgagc aaatcccatt ggaacaaaag tactatcaaa tccaatagta
    43021 atagacttgt tagactggta ctttgaccaa ttatctccac tagtttcgct agaatttttc
    43081 ccacaagcta ctaagaacaa agcagtcatc agactgacta atacaagcat ccattttttc
    43141 atccattttt cctcctattt aggttctact ttcaataata catctgcgat attttcagca
    43201 aactgcaaat catgggtaac cacaatctgg gtcatcccaa gttccctatt ttgcaagatt
    43261 agcttctcca cttccaaacg taattctgga tccagggcag aagttggttc atcgtagcca
    43321 atgatttctg ggtcaatcat catagcacgc gccaaagcca cccgctgctt ttgcccacca
    43381 gatagtgaga aaggataggc ctctgcgtgt cctcctagtc ctaactgttc caagagtcca
    43441 ctcgccttct tctcagcctc ttcctgcttc attcccatgg tcttcacagg cgataaggtc
    43501 aaattgtcca gaactgataa atgagggaac agttgaaaat cttggaatac aaatcccagt
    43561 agattgcgct tctgcaattc atccagctct aaaggttgtc cattataaaa gatttgccct
    43621 gaatcaatgg tttcaagacc tgcaagcata cgtaaaagag ttgtcttacc tccaccagaa
    43681 ggtccaacga tagccaggat ttgcttttca ggaatactta gactgaaatt agacaggatt
    43741 tgtttgtctc caaagacttt attgatattt cgtaattcta acatggcagc ctcctatcta
    43801 taataactat acttcttctc aacttttttg gaaataattg tcacaatccc aatcaaaatc
    43861 aaataaatgg ctcctgccaa gaacatagga actagactag catcgcggtt agcagctgtt
    43921 cgactagcca agataaggtc tgaaattccg agagcataga ccaaagaagt atccttgacc
    43981 aaactcataa cttcattaaa gacactagga agaacgatct tggtcacttg gggcaagata
    44041 atatagcgca ctctgtcaaa agggctaaac ttcaagacct tggcggcctc atactgtcct
    44101 cttggaatag tgtcaattcc cccacggaaa atttctgcaa agtaagctgc atagttgaga
    44161 acaaaggcaa taatagctgc aggaaggcgg tctaaacgaa tcccaatact tgggagcaca
    44221 taatagataa aaatcagttg caagagtaag ggggttcctc gcataaccca gatgtaaatg
    44281 ttaatcagat aatggagggg cttccaatgg acttgcaagg caaaggcaat caaaacgccc
    44341 aagggaatcg aaaatagcaa gaccagtgca aagacctgta cagtcgtgct cgcaccattc
    44401 agtaaactcg gtaatatctc aaataaataa gacatactgc acctcctaaa agtaattgtt
    44461 cctattatag catgaataaa caagattacc aagtattttt gtaaaaagat tctaccatat
    44521 acaaataaaa accgtagcta tcaaaagaga taaactacag ttctatatgt ttaaacattc
    44581 ttacaatctc tcaaacagtt cttgcatttg cacatcatct ggaaccagtt ttaagtaagt
    44641 gtgagcatgg acttttgctt cttcaaaatg tcccaattca cgcaagagat agatatagtg
    44701 ttccagaaat tctggattgt ccttcaaatc tcctgtcaac tcttgataat gctcataagc
    44761 agtatccaaa tcgtccattt cttgataaga acgagcaatc atccacttgg tcaaaagatt
    44821 ttctggctcc tcactctgca agtctagaat atcctcataa cgctcctgct ccagataaat
    44881 agtggctaaa cgaagcaaga tttcttctgt atcctcagcg tcttcttttg cagtaaggag
    44941 ataattttct gcaccactag catcatgtaa ttcataagaa aattgtgaag cagctagcaa
    45001 gaggcgagtt tcaaagggat ttttctctaa tccttgctta gcgatacgca gggcttcttg
    45061 aacttgatgt tccttatgta aagcctgact gtacccatac tcatagcctt caaagtcagg
    45121 agaaatggta tcaagctgct taaagtagag ggtggctttt tgatattctt cttgatcaaa
    45181 ataaagactg gccaactcaa aagctgttaa gtcatcgtat tctaactcca gggctttttc
    45241 taaaaactca gtagccgttt caaatttccc taactgagca taggcaaagc caattcgttg
    45301 ataggtggaa atgcccgttt gctcataaat cgagctatta tctaactggg cataggcttg
    45361 aatagccgct tggtaatttt ccaactcact atccaactct gccaaaccca atatcaagag
    45421 agaatcctct gagtaggtca aggcctccaa taatttctca cgtgccacat ctgtcaaacc
    45481 ttccagctgg taaaggtctg ccttcagagc caaagacgag acataccagt cactgtcagc
    45541 ttggatttcc tcaagatagg taaaggcttc ttctatttga ccatcctcgc tagcaattgc
    45601 agctagatta agatgaacct ctggaaaatc ctctacaatt ttcaggtaaa tttccttggc
    45661 ctgaggatag aaaccaatcc cttcaagata agttgccaac tcatacagaa gatcacttga
    45721 atcattttct aaagctttgg cgaaataatg ctcagcctta gttaaatctt gctcctccaa
    45781 agcctgtaac atctgttgac tattgttcac tcttgacttc ctcaccctct aattcatata
    45841 gaccactgac taccttatac cattcgaaaa tggctgaaat gacaacctta gcagaggcat
    45901 aaaccggaat accaagtaaa actccccaga taccaaacat agatcctgaa gttaacaaaa
    45961 caaagagaac attaataggg tggatgttta attgacttcc caaaatcaat ggagagacaa
    46021 aacggccttc aatagtttgt tctacgataa agacaatcac tactttcaaa agcatgactg
    46081 gaccagcaat caaacccaat actagagcag gaagcatggc tagaaagcta ccaagataag
    46141 ggaccagatt taaaatacca gcagtaaccc ccagcgtaac cgcatagcgt agaccaataa
    46201 tcttgaagaa gatgataaac attactgcta caataatagc cactgtcact tgccctcgaa
    46261 catagttgga caactgttga ttcacatctg ataaaacttg tccaacaggt tccttcaatt
    46321 ttcttggaat gaattgggtc aaatagttac gcaagccttt cccatcacgc aagagataaa
    46381 agagcatgaa aggaacgata atcaaggcaa caatcacttg agaagctccg ctaataaagg
    46441 cactcaccca gttgactgcc tgagatgaaa ccttacttgc caaaactgta gcctggctag
    46501 aaaaattggt caaaacttgc tctaattgag gtctgaaatc atctggcagg tgctgggcta
    46561 ccaatccatt aacaatccta tctatatctt ctaagtaaac aggaacgttt cttgcaaagg
    46621 tcaaaacctg acgttgcaga tttggaatgg cgactgccaa gccccaaatg ataaagagag
    46681 cgatgataac aaagacaata gtgatagcta taacacgatt aaccttatgc ttctccatcc
    46741 aatcaacaat aggattcaac aaataatata acaaaccaga taaaatgact ggcaacatca
    46801 caactgctaa aaagtctaaa acaggtgaaa atagaaaact aatcttactt aaaataaaaa
    46861 gattcagtcc caataataag gttactaaaa ataccgtaat tgccttgtta tctaaaaacc
    46921 acttgaaaaa ccaagatagg ctaaaatgtt tctctttttg ctccataaat acttcctttc
    46981 tattgttctc tcattatacc atttttgaaa caaaataact cttttttaaa gtgcttacat
    47041 ctaattggat gtctttaatt tgcttgtttt tgtgatataa tagtcttatc tttacacaga
    47101 ggtatagtaa tgaaagaaac tgtttatttt ggaacttata cacgtcgtac ttctcaaggg
    47161 atttacaagg cagactttga tacagaaact ggtcagcttt caaatctaga actttttgca
    47221 gctgagccaa gtccaaccta ccttgccttt gaccagcacc aacatttata cactgttggt
    47281 agccaagacg ataagggggg aattgcagcc tatcaaactg acgggactgt gttaaatcat
    47341 gttgttgaag aaggagctcc ccactgttat gttgctgtcg atgaaaagcg tgatttggtt
    47401 tacgcagcca actatcacaa gggacaagtc cttgtttata aacgccagga agatggtagt
    47461 cttctactta gtgatatgga tcaacacagt ggccaaggtc cacatgaaaa tcaagcttcc
    47521 ccccatgttc actatacaga tttaacacct gaccactatc tagtgacctg cgacttggga
    47581 actgaccaag tcatcaccta tgacctcgat caagaaggaa aattatctaa gctctatacc
    47641 tatcacagca agccaggagc aggctcacgc catatcattt tccataacca ctataaaatc
    47701 gcttatctca tttgtgaact caatagtact atcgaagttt taatctacga tggcgttggc
    47761 gaatttgtac gtatgcaggt tatttcaact ttaccagaag cttacgaagg ctttaatgga
    47821 accgctgcta ttcatctctc taaagacggt aaatacctct acgcttctaa ccgtggccat
    47881 gattctatcg cagtatatac catccttgcg gacggtagct tagagttatt agaaatcgtt
    47941 ccaacgcatg gtcagactcc acgtgatttt gatttgacac ccgaccaaaa atttctcatt
    48001 gttgtccatc aagactctga caatgcaact gtctttaaac gtaattgtga caatggtcgt
    48061 ctagcagaac tctccaacga attccatgtt cccgaagcag tctgcatccg ttttgctcct
    48121 taaaacacca taaaaaatga acttggtatt ttcaagttca ttttttctta tagtctattt
    48181 ccgacattaa tacggttaat ggcacgttgc aaagcaatct tagcacgacg ttcttggtca
    48241 atcaaatgtt tgtcttgtgc ttcttcaatt gcacgttctg cacgaagttt ggcacgttct
    48301 gcacggctaa tatcgatatc acgagcacgt tctgcagagt cagcgacgat tgtgatcata
    48361 tcattggcaa tttcaataac gccaccgttt actgcaatcc agttcacgtg atctttatca
    48421 tcgatacgtt ttacctttac ttcatcaact gctaaaaccg caatcatatt ttcatgtcgt
    48481 ggcaagatcc ccatctcacc atccagagtt cgaaccgata catagctggc atggtgatca
    48541 tagacgagac catctggtgt cacgatctgg acagttaact gagccatagg tcacctctta
    48601 aaatcccatt ttttcagctt ttgcaatcac atcttcgata gaacctacac cacggaaggc
    48661 atcttctggc aagtggtcgt atttaccatc aaggatttcc ttaaagccac gtacagtttc
    48721 agcaactgga acataagaac ctggctgacc agtaaattgt tccgcaacgt tgaagttttg
    48781 tgacaagaag aactggatac gacgggcgcg agcaaccaag gtcttttctt catcagaaag
    48841 ctcatccata ccaaggatag caatgatatc ttgcaattca tggtaacgtt gaaggacacg
    48901 ttttacttca gcagcaactg catagtgctc ttctccaacg atttcaggtg ccaaggcacg
    48961 tgagcttgaa gcaagtgggt caacggctgg gtagataccc aattgtacca acttacgttc
    49021 caagtttgtt gttgaatcca agtgagcgaa ggctgttgct ggcgctgggt cagtatagtc
    49081 atccgctggc acatagatag cctggataga ggttacagaa cccttcttgg ttgatgtgat
    49141 acgttcttgc aattgaccca tttccgtagc aagtgttggt tggtaaccaa cggctgatgg
    49201 catacgaccc aaaagggcag atacttctga accagcctga gtgaaacgga agatattatc
    49261 gataaagaga agcacgtctt ggccttccac atcacggaag tattcagcga ttgtcaaacc
    49321 agtaagggca acacgcatac gtgctcctgg tggctcattc atctgaccaa agaccatggc
    49381 tgttttctcg ataacgcctg attctttcat ttcccagtaa aggtcattcc cctcacgagt
    49441 acgttcccca acaccagtaa atactgaaat accaccgtgc tcttgggcaa tgttgtgaat
    49501 caattcttgg attaagacgg ttttaccaac tccggcacca ccgaaaagtc caactttacc
    49561 acctttaagg taaggggcaa gaaggtcaat aaccttgatc cctgtttcaa ggatttcaga
    49621 agaggtagac aactcatcaa aagttggagc ttttttatga attggctgac gctctgcgtc
    49681 ttctgtaaaa ggagcttcca aatcaatggt atctcccaaa acgttgaaga cacgtcccaa
    49741 agtttcttta cctactggta cagagattgg acgacctgtg tccaatactt ccattccacg
    49801 agtcaaccca tctgttgatt ccatggcgat agtacgaacc ataccatctc ctaactccaa
    49861 ggctacttca aggacgattt ttgtttttct ttcgtcattt ttgtagacga caagtgcatt
    49921 gttaatctca ggaagttttt cccctgctgc aaacaaaacg tctacaacgg gaccgataac
    49981 ctgagcaatt ttacctgaac tcatctcctt ctcctattct atataagata ctgtcggttc
    50041 ctagctcaac taggtcctaa gttcattttc atacgagctg gactagagcc tattctaagg
    50101 cactagcacc tgctacgatt tctgtaattt cttgtgtaat cgccgcctgt ctggcacggt
    50161 tatactgaat tgtcaaatca ttgatgactt tcttagcatt atctgtcgct gtttgcatgg
    50221 ctgtcatacc cgcagcattc tcagctgtct tggcatcgat aatggcaccg taaatcatac
    50281 tttctgcaaa ctgaggcaac aactgctcca gaatttcttc tcggctggtt tccaattcaa
    50341 aagtcaagct gtactcttca tccgcttcat ttggatccaa gtcaacaatc ggaagcattt
    50401 gttccacacg catttgactg gttagcgtat tgacatggtg gttgtagcaa acataaagct
    50461 catcaaagag ttcattttgg tacatttcaa cagtttttga aataatctta cgaacttgat
    50521 caaagctagg ttggtctgcc aagccacgta attcataaag tggttgaata ccgcgagcct
    50581 taaagaaatc agctcccatc ccaccgatac agatcatttc aaaaccttta ccgtctgggt
    50641 ggtattcttc tttcaactcc ataacagctt tcaaaataga ggaattataa cctccaacca
    50701 aaccgcggtc tgaagtgata acgatatagc ctgtcttctt cacagaacgg ctaatcaaca
    50761 tcggattagt tgaagcacca gctccattac catgaaggat atctgtcaaa agtttacgca
    50821 ctttctgagc gtaaacttgg aagttgcgag cagcttcttc agaacgacct agcttagcag
    50881 ccgataccat ttgcatggca ttagtgattt gactcgtatt ttttgttgag gcgatttttg
    50941 ttttaatatc atttagagat actgccatct gacacctcta ttcttattgg aagctagatt
    51001 gattgagaaa ctctgtaatc gcagcatcca agactgcttc ttctggcaag tcttttgtat
    51061 cacgaatggt ttccaaaatc tctggatgtt gagcatcaaa gaaggtatgg aactcttcct
    51121 cgaaacgaac aatatcatct actggaacag tatccaagaa accatgtgtc aaagcataaa
    51181 gaatggttac ttgtttctca acaggtaatg gtttgtgaac aggttgtttc aagacctcaa
    51241 cggtacgacg tccacggttc aacttagcct gtgttgctgc gtccaagtca gaaccaaact
    51301 tagtaaaggc ttccaactca cggtatgaag caaggtcgat acgaagtgta ccagcaacct
    51361 tcttcatggc tttgatttgt gcagaaccac ctacacgaga tacagatgaa cccgcatcga
    51421 tggctggacg aatacctgca ttgaagaggc catcgccaag gaagatttgt ccatcagtga
    51481 tagaaatcac gttggttgcg atataggctg agatatctcc tgcttgtgtc tcgataaatg
    51541 gtagggctgt aattgatcca ccaccaagtt catcagaaac tttagctgag cgctcaagca
    51601 aacggctgtg gagatagaaa acatcccctg ggaaggcttc acgacctgga ggacgacgaa
    51661 gcaagagcga tagttcacga taagctaccg cttgttttga tagatcatcg tatacaatca
    51721 aaacatgctt accttgatac ataaattctt ccgccatagc aaccccagta taaggagcta
    51781 ggaagagcaa tggagatggt tgtgaagcag aggctgtcac aacgattgtg tagtccaagg
    51841 caccgtactg acgaagtgtt tctacttgcg tacgaactgt tgattctttt tgtccaatcg
    51901 cgacgtagat acagatcata tcttgatctt tttggttcaa gattgtatca atcgcaatgg
    51961 ttgttttccc tgtctgacgg tcaccgataa tcaactcacg ttgaccacga ccaatcggta
    52021 caagggcgtc aatagctttc aaaccagttt gcaatggttc tgaaacagac ttacgttgca
    52081 taacaccagg agctggtgct tctactggac gagttttatc agtgtggatt tctccaagac
    52141 cgtcaactgg acgaccaagc ggatccacaa cacgaccaat cagactttca cctacaggga
    52201 cttccatgat tttccctgta cggcggattg tatcgccttc acggatatct gtaaagtcac
    52261 ctaggatgat aataccaacg tctgttgact ccaagttttg agccatacca taagagccgt
    52321 tttcaaaatt caacaactct ccactcatga cattttcaag gccatgagca cgcgcgatac
    52381 cgtccccgat ataggttaca acacctgttt cagtcacatc aaaattgggt ttgaaatttt
    52441 caatttgttg cttaattaaa gcgctgattt cttgtgcgtt aattgccaaa agaacaccac
    52501 tttctatttc aaattttctt taacaacttt aagttgttgt ttaatactca catcaattgt
    52561 cttgtgattg gcaaaaatga caaaaccacc aatgagactt tcatcgattt gttcttttac
    52621 actccttact ttcagagaca tttttttctc aatcaaaggg agcaaacgag tcttctgttc
    52681 atcagttaga ggatgagcag acgtaatcgt cacttcaaat cgatttgttt ctttttcaag
    52741 tcggttcaag caatctacaa gcacatcata aaaaagattt gctctgtgat tgtaggccag
    52801 aacctggata aagttttgta ataaaggcga cacagaatct tggaaaaaag caattgtttt
    52861 ttccttatcc gactcgtcta ctgccacttg ttttaaaaaa gaaggcagac ctgttttttc
    52921 aacaacttgc ttgatttgag tcaagtctga aaagatacgg tcttcttctc ctttttcaag
    52981 taccaattgg acaaaaggca tgctgtattt ttcaattacc tttactgttt tcttgtccat
    53041 taagcttctc ctagctgatc gatatactga tcaatgagtg ctttatgggc atgactgtca
    53101 aggttttgtg agatgatttt accagctaag ctgatggtca aatctgcgac ctcaccctta
    53161 acactctgta aagcttctac tttattttga gcaatttctt gattggcttt ttcttttaag
    53221 tgtcctgctt ctagtttagc atctgctaag atattagcct tactttgctc agctgtttcc
    53281 tttgcatttt caatgattgt cttagcttct ttacggctac cagccaattc atcttcgcgt
    53341 ttttgagcca atacttctgc tttttgacgg gcttcttcag ctctatcaat atctgaagca
    53401 attttttcag ctctttcttc gaaaatgcct gtaatattag accatgcaaa ttttttaatc
    53461 aagactagca aaagaataaa agagccagtg attaaaataa aattaccaat taattcacct
    53521 actgttacgt gcatcagtta ctcctttcta ttcctcttca atattaatct tattccctaa
    53581 atacacagat gtcaaaagag taaaaacata agcttggatg caggaaataa agacagaaaa
    53641 tgcagtccaa gccaaattag ctccaaaggc tactggatac caataaatag cttggtggga
    53701 aagaagaagt aacaaactcg tcatgacctc tcctgcaaag atattcccaa aaatccgcaa
    53761 agccaaagat aagaagtttg taaattcttc caagatattc atcggtatga caaaaacagg
    53821 actcataaaa gattttatac tttttttaaa tccacgacga cgaacgcttt ctatatgtgt
    53881 caacaaaatg acaagaaaag ataaggttaa gtcatactgt aaattagcgg ttggcgaact
    53941 ccaccagtta gtcccatcga tcgtttgaag ctttgtcatt aagccaaggt tattggcaat
    54001 caccatgaaa aggaataaac aaaggaaaaa gagtgagtaa tctttcatgt agcgcgaacc
    54061 aatgttaggt tctgtaaatc caataacaaa gtcatagaca tactcaagta tattttgctt
    54121 tcctttgggt ttcaaggtca tattgcggct tgcccaataa ataaagacaa aaataactcc
    54181 cacaatcaac aaagtcatgg ctaacatagt cagattgaag ataacaggac caatagagat
    54241 gattggatta atactttctt ccatctaaaa tcctcccttt tcttctcgtt ccatgtattt
    54301 atttgataat aaatgagaag acaagagtta caaagaaagt tccttcaata aaggcaacac
    54361 ctaaaaacat caaactacga aactcagaaa gcatatctgg ttggcgtgct actgatttaa
    54421 acagtccatt catcaataaa ccttcaccga cagatacgcc catacaggca atacataagc
    54481 ctaaaaatgt taaattcata acaaattctc ctttaaaata aaatttactt acttagtgta
    54541 gtcctaaaat ttaaatttgt caacttttaa ccgctattgg aagcgtttaa taaaaattat
    54601 ttccattttg ctaaatttca agttcaagtt agccatccag aagtcttctc tgggtgactt
    54661 gtagtccaag cattttttag gatagttgtt aatccacttt tcgatgaatg cgacttcttt
    54721 gggagtcatt ttcttggttc ccttaggtaa ctatctacga atgagcctgt tgtgattctc
    54781 attagttccc ctttcccaag aggcataggg gtgcgcataa tagatgtgct cctcagaaaa
    54841 tatatcaaac aagcgattga attccgttcc attatctgcc gtgatggaaa gaatcttgtg
    54901 ttgttttaag atgagtttta gagcctgatt gaccacctca gcacttttat ttggaatcaa
    54961 tcggatgatc tgatgtctac tctttcgatc cgtcaagaca agcaagcagt agtttttcgc
    55021 tctcgtaagt agaaccgtat caatctcata atgcccattc tccaagcgaa gattgatagc
    55081 ttcaggccgc tgtgcgatgg attgaccagc aggtttaaag ttgatgctag cctgtttctt
    55141 aagcgctttt ccttttctag ggtaaagcag atcctgtttg cttaacccca attttccatg
    55201 atgaatccaa tagtaaatgg ttgaaattcc cacgttaacc cctttagcca tcaccatcat
    55261 ttcaggcgaa aatttttggt tatgatagtg gagaatcttt tcctttagtt ccttggtcac
    55321 gcttaatttc ctgaccgagc gcttgcgatt gttttcataa gactgttgag catagtcggc
    55381 agaataaacc tctttgaagc gcccttttcc aagacattgt cggactgtcc cacgcttgat
    55441 ttcagtgtgg atagtttgag gaacttttcc aagcagagag gcaatttctc tatttgattt
    55501 ccctttgttc gccttttgta gtataatggt tttgcatctc tgtgcctttc ttgtgtttgt
    55561 ggttgaacaa caagtataac acaaaggtgt tttcttatgc ctacaagagc tttcattatt
    55621 tccattttct tttggatttc actctattct gaaaaacttg tgtatattta ctgaagctag
    55681 caagtcttac ctgtaaattt aatgaaagca acacaaaatc cgagagggga atctcggatt
    55741 aatgtcaatt taaaaagata gagagttttt agtttaaata aattgtttta aaatatcaac
    55801 aacatcactt cttttcttaa cctgataagc cttgattcct aattttgggg ctacgattat
    55861 attgtcctca atatcgtcta gaaagacaca atttctaggt tataactggt atttatcgat
    55921 agttactcat atatatcagt ccacctccat actcacctta cagattcttt tgataataat
    55981 atctttgcct aatgtagaga tagtcttgca aagaaaaaac ttccttgtag ccatgtttct
    56041 gataaaagtc cggtgcctgg aactggtaag tattgacaaa ggcaaaacaa caatttcgat
    56101 tcttagcttc actttctgcc tgttgcaata gttttgaacc gattccttgc cctcgcagtt
    56161 cctcttttac aaacaaatac tcgatttcta gccaatttcc aaaagtctct gctatcaaac
    56221 ccgcaaggag attgcccttt tcatcttcga cataaagatt aagtggctca ctttcagcct
    56281 cttctctttt tgaacggtta taaacacgaa tcagattccc tatttcttgc gatttatgtg
    56341 attccttatt ttccaatcta aagtataatg aaatgaaata aaacatgcgc aaatcgatta
    56401 aggaatttaa tctaatttct aacaatgtct tagaaatcaa agtgtactat tttaacttca
    56461 atgcactata catctaatac tcaataaaaa tcaaagagca aactaggaaa ctaggcgcag
    56521 gctgctcaaa acaccgtttt gaggttgtag atgaaactga cgaagtcagc tcaaaacatg
    56581 gttttgaggt tgtagatgaa actgacaaag tcagtaacca tatatacggc aaggcgacgc
    56641 tgacgtggtt tgaagagatt ttcgaagaat agcaaaatgg aaaaaggagt gagtgaagca
    56701 catcgcctcc ccactccttt ttctgttttt aggctgtttt ttcaaccttc aagattttta
    56761 catcatagct accaacaggc gtttcaatgg ttgctgtgtc acctgttttc ttgccaatca
    56821 aggcctgccc aattgggctt tcatttgaaa ccttacctgc aaaggcatcc gcaccagctg
    56881 aacctacgat aatataaact tcttcttcgt cctcaccaat ttcttggatg gtgactgttt
    56941 taccaatcgc tacttcgtcc tgggcaactg cgtcgctatt gacgatttca gcatagcgga
    57001 tttttgtttc taagctagag atttgtcctt cgacaaaggc ttgttcatcc ttagctgctt
    57061 cgtactcact gttttctgaa agatcaccgt atgaacgggc aatcttaatg cgttctacca
    57121 cttctggtcg acgaaccaat ttcaattctt ctaattcttt ttcaagtttc tccttttcct
    57181 caagggtcat aggatatgtt ttttctgcca tttttctcaa ctttcttctg ataatatttt
    57241 ctaaagaaaa ttatgtgaag tatcacataa ttttagtttg tttagtttaa tttgctgttg
    57301 acatgttcag cgacattgcg gtcgtggtct tcttgattgt tagcatagta gaccttgcct
    57361 tctgtgacat ctgctacaaa gtagaggtta tcgctcttag tttgattgat gcttgactca
    57421 atcgcatcca gacttggact atcgactgga ccaggcatga gacctacatt tttataaaca
    57481 ttataaggtg aatcaatgtc ggtatcaatc gcaacatcct cagctagact gatattttgc
    57541 cccagttttc cttgggcata caagattgca atattacttt gaagtggcat atcacgattt
    57601 aaacgattgt agaatacacc tgcaatgagc ttacgatctt ctgtcttggc accttctttt
    57661 tcgaccaagg aagcaatggt caacaactca ttgatagtca agtttttaga tttgatagta
    57721 ctatagtaag gagataggtt cttatccata gcagctaaca tctcatcaat caagctctca
    57781 atagttgtgc tttccttgat agagtatgta gctgggaaaa ggtatccttc caaacgataa
    57841 cgcgcaccgc tgtcttttac aggcaaactt tccagtaaag taggatattt cgctactgct
    57901 tgactgataa acgtctcatc ttgaacttta gccaagaaag cctccgctgt caaagactct
    57961 ttgaagtcac cttgcaattg acccacagtt tgagcaatct gatccaaggt ataaccttct
    58021 ggaattgtca aagtcgcaag tacaggttct tgcggttcat ctgttccacc tttttgcaac
    58081 tctttgagta agtcttctgt actcatactc ttttgcaaat tgtagtaacc tgctttcaag
    58141 tcggtataat ttttatactt ggcataaaaa ctaaaaatca gaccatgctt taccaaacca
    58201 gctttttcaa gcgtcgtacc gatttcttga acgtttgaac cttctggaat tccaaccgtc
    58261 acatatttct tagaattagc atcgataggt aacaaagaat ctagcacgta ctggtaacca
    58321 aagtaaccac ctgctgagag caaggcaagg aatacaagga atgaaatcac aaaagctttc
    58381 acatgtgact tcttctcttt cttaggttta acgggttttg ctccttctct acggctacga
    58441 cgtgatgtat cgctgatgat atccactgtt tctgtagcag gcgtaggtgt ttctggacct
    58501 gcttgttctg cttttttctc tgccgtcgta taggaaacag ctacccttgt tggggtttca
    58561 ttgtattctc tttcaagttt cttaggtcta acaggacctg gacctggtct tgatccactt
    58621 tcttccgctg gagaagaagg tacatcttga cttggatgac ttggaacacc aggagtttct
    58681 ctttgaatct catctgctgg agaagctggt acaccttgac ttgggtgagt aggcacggta
    58741 ggagcttttc tcataatctc ctctaccgtt gacaaggaat cagccatgag ttcttcagct
    58801 gaaggttcat ttgcaggagt gcgaactact gcctcatctt ctttcagaac ttcatcatag
    58861 ccttttactt tttctaaatc tctcagaatc tgctctttaa agcttaattt ctcttcttct
    58921 cttgactttt cactcaaaag tttttcctcc ttgttgataa tccataatat tatatcttaa
    58981 aagtccaaaa aaagcaatct atgatacttt tcctaactta ttttttcatt tcccagacca
    59041 tatcatacca tgtttcccct gcaaaggttg actgggactg gccttcgttg acaaatccat
    59101 gtttttcata gtaggcgatg agatagtcat gacaggttag attaatgcca tctctctcat
    59161 cttcaagagc aacttctttc aaggctgtca gcaatttctg acccagtccg agtccctgtg
    59221 cctccttggc aatagacaga caggtcacag agatataacc accaggctca tgactatagt
    59281 cttttatttc ttctgtaaaa gactggtctt gcagatggcg gtgcaggcca actggtcctt
    59341 cgatataacc catgattctt ccttcttttt cagcaaccag aaaagaggtc tgaatttctc
    59401 tcaaatgtgc ttcaaagaca gaaggaggaa tggcttcttc gaccgaaaaa ttttcaaatt
    59461 caagttcaac aatccgatcc aaatcttcta atcttgcttg tctgattttc attgttcctc
    59521 cagataaaag ggattaaacc aaatcatact atagccctgg ctagttgcat agagcaaagt
    59581 ttcttcttca tcaacaaaac cgttcatttc aaaataggaa agcagctcat caggactctc
    59641 caaacgaatc cctttgcaat ccagctcaac tgccacctct ttcaaggctg caagaagaag
    59701 tgttcccagg ccctgtctct gatggtcaga ctcgatgact aaagaatgta cttttagaca
    59761 ttgcggattg tctgactggg gacttgataa aatatagcct aaaagttgat tttcatccct
    59821 agctagaaga aaggtatccg cacacttacg gatactttct tctaaaatat gggaaagttg
    59881 ctgcttttca gctggaaaag acgaggtctg aagtgcccct atctcaggca aatcagactt
    59941 gcttgcctga atgatcttaa ttggaatttc catgggaaca tcctattgaa cattgcttgt
    60001 caagttagat aagagacgct caaatgagta ttcataggtt tggatgtctc ctgctcccat
    60061 aaagacgtaa acagcattgt catggtctag gagtggagaa acattttcaa cagtaatcac
    60121 ttggtgtttt ttgttgattt tatttgctag gtcttctacc ttaacgtcac catgatctac
    60181 ttcacgagcc gagccataaa tttgcgctag ataaacagca tctgcttggt ttaaagcatg
    60241 ggcaaagtcg tccaacaggg caatggttct tgtaaaggta tgcggttgaa agactgctac
    60301 aatttccttg cttgggtatt tctgacgagc cgcatccaag gtcgcaataa tttctgttgg
    60361 atgatgggca aagtcatcaa taatcactgt atcattgaca attttctcag tgaaacgacg
    60421 tttaacaccg gcaaatgttt tcaagtgctc acgcaccaag ttcaaatcaa atcctgctgt
    60481 gtaaagaaga ccaataacgg ctgtcgcatt catgatattg tgacgaccaa aggttggaat
    60541 gtggaattgc cccaagtttt gtccacggaa atgaacggtg aaggttgaac cagttgttga
    60601 acgaagaaga tcactagcta caaagtcatt gccttcagct tcaaaaccat aataataaat
    60661 tggtgcatca gacgtaatct tacgcaattc agcatcttca ccatagacaa aaagaccctt
    60721 ggtaatttgt ttggcatagt cgttaaaggc attgaaaaca tcctcgagac ttgtgaaata
    60781 atctggatgg tcaaagtcaa tgttggtgat aatagagtat tctgggtggt aaggcatgaa
    60841 gtgacgctca tattcgtcag attcaaagac aaaatatttg gcattagccg aaccacgacc
    60901 tgtcccatct ccaatcaaga agctggtatc tgtaatgtga gacaagacat gagacaacat
    60961 acctgtcgtt gaagtttttc catgtgctcc tgctactccc atgctaacaa agtcacgcat
    61021 aaagctacct agaaactcat ggtaacgttt gtagctgata ccattttggt ccgcataggc
    61081 aatttcgacg ttgttatctg gacgaaaggc atttccagcg ataatttcca tatcaccgtc
    61141 tagattcttt tcatcaaaag gaagaatggt aattcctgcc tgctcaagac cgcgttgggt
    61201 aaagtagtac ttttcaacat ctgatccctg aaccttgtgc cccatctggt gcaacatcaa
    61261 ggccaaggca ctcatccctg atcccttaat tccgataaaa tgatatgtct ttgacatgtt
    61321 ttctccccta ttctgtcatt ctggtcagat tcaactcttg ggcaacccga cgttcttgtt
    61381 ctgtttgttt acttttttta ttgtagattt ggctcttctt tagaaaatca taattgtttt
    61441 tctttggagc aggtgctgac acttcttcat tcttggtagg gatagaatga acttcttccg
    61501 ccaagatata atgagactgg gtcaattttt ggctatattt gacaaattca ccaggatttt
    61561 ccttttggaa aggagttgtc ggttgattgc cctgtctaac tagactgggc tgagaatgac
    61621 gtctcgcaag gctgaaatcc tgagttaggt agttagcaga gcgtttcttt ttcaagtccg
    61681 cacgcgcttc ttcacgcgcc acctccgcat agctctttcc ttctttttta acccctaaag
    61741 gagccttttt aggtttttcg acttgctttt caatcggttt tactggtttt tcttcagtaa
    61801 taggagccca ttctaaataa tttttatctc gatactcacc cttgatatta ctgatcagat
    61861 cagactcatc atagagattc atgactggca tttcagtcaa catgacctcg tcatctgaca
    61921 ccaatggaaa tcgttcttgt ttcattttct atttcctttc aacacttcat tatagcgtat
    61981 tgtcttgatt tttcaagtgc tggcttcaga aattcccaaa atttctctaa tttctgctag
    62041 ggtcagacta ccacgtgact ctgtgccatc caatacttgt gacaccagat gtttcttttg
    62101 ttcttggagt tcctgaattt tttcttcaat ggttcccttg gtcaccaagc gatagacctc
    62161 aaccgtttct tcctgaccca tccgatgggc acggccaatg gcttgcgctt ccaccgcagg
    62221 attccaccaa aggtcaacca agatcactgt atctgcacct gtcaggttca gaccgacccc
    62281 accagccttg agggaaatca gaaaggcatc tctttctcct tggttaaagg ccttggtcat
    62341 gtcttgtctt tccttggctg gggttgaacc cgtaatttta aaggatgtca ggcccaagtc
    62401 tggcagttct tgttcaattt tttccaacat tcccttgaac tgagagaaaa tcaagacacg
    62461 gtgtccgccg tctgccacct gtaccagtag gtctcggaga ctatctagtt tgccgctggc
    62521 tccctgataa tcttccataa acagggcagg agtgtcacat atttgacgca agcgcatcaa
    62581 accagataaa atttccacac gacttcgctg aaattcctgt tctgacactt gagccagatg
    62641 gtctcgcatc tgttgtaact gggcaaggta aatagccttt tgctggtctt ccagttcatt
    62701 tttataaacc acctcaatca agtctggcaa ttcagtcaga acttcttctt tcttgcgtcg
    62761 catcacgaaa ggcttgataa actgagccac tcgctctgct ggcaatttca taaattcttt
    62821 cttgcttggc aaaagtccgg gcatgacgat ttggaaaata gaccacaact cacccagatg
    62881 gttttcaatc ggagttcctg acaaggcaaa gaccgacggc accacaaatt gtctcaaggt
    62941 ctgggcaatc ttggtctggg catttttcat gacctgagcc tcatctaaga aaaggaagtc
    63001 aaaggccatc ccctgataaa actcactgtc ctgacggaag gtggcatagc tagtcacata
    63061 gatttgatgg ctctcggcaa gaatctcctc acgacttgct ttcaaaccat gaacaacagc
    63121 cacatccaac tgtggggcaa atttctgaaa ctcatctgcc cagttgtaaa tcaaacctga
    63181 cggagcgaga atcaaaaccc gactttcttt tgtcacttga ctagtcaaaa aagcaatggt
    63241 ctgaagggtt ttcccaagtc ccatatcatc agccaaaatc ccaccaaaac cataatgatg
    63301 gagcatctgc aaccagccaa ttcccttttc ctgataatct cgcaagtcag cctgaacctg
    63361 agttgcctgt cgaggaaagt cctctggatg tgtcaaatcc tgggccagat tctggaattc
    63421 ttgtgaaaaa gaaacacggt ctcgcccttc aaagagatga gctaaactgt aggccaagga
    63481 tttccgagcc tgcaaggtcc catcttttaa ttcaaattgc cccagttcct gtagattttg
    63541 gcgaattttc ttggtttctt catcgaaaaa gtaaacttga ttagacgaat caatataaaa
    63601 atcctgattg gcaaccaagg cctgcatggc ttggtcgatt tcctcctggg caatatcttg
    63661 aaaatcaaac tggatttcca agagacctcc cttggaggca atctgcacct gaggactcgc
    63721 taggctataa agctcttcta gtttatctga taggtcaaca tgcccgagtt tttcaaagac
    63781 tggaatgata tcatgaaaaa aatgatagac agactccgct tttaaggcct gacgccaaga
    63841 ttgaaaatcg gcttcaaagc ccgcagccaa acagacttgg aaaattcttt cttctaagtc
    63901 cgcgtcactt gaaaatggta attcttctag ctcttgtcgg ctagatacct gtctatttcc
    63961 ataatcaaac tgaatttcta aacgaatccg attatcttct tccctgtcaa agtaaaaaga
    64021 gggcgcaaaa gttttgattt gtagacgttc tggagctgaa acggtgccca tctggataaa
    64081 aagagtcaga caggaggcca atttgtctcg atcactgcta tcaaattgca ggtatttctt
    64141 tccttgttga cccacaggta acgctttaat ttccttgaga agacgcatct gctggtctgt
    64201 taaaaaataa acctgacctt tatggaaaag tactgctccc tgataaaaga cattgaccct
    64261 aggactctca ctgatttcca tttcaaaata gtccgagtat tctgttactg taaaggcaaa
    64321 tagattggca tcagcatgca tatcctgaaa aagcagggtt tggtagctat ccacttgatg
    64381 gtcaaattga aaatggggca aggccatcag taaattcaca ccctgctcaa aaaaggtcag
    64441 agggaaaaag aggtgccgac cttggttttg gaaaaagagg tctgaaacca gcccttcctc
    64501 cgttagtccg tgcaagaaag tcaaaagttc ttggctggca tcatcaaagg cttcccaaga
    64561 aagagactcc tcataaatct tgccaatcat atacgacttt ctctgctcga caatccttaa
    64621 aaaaagtgga atatcccgaa tgacatagta tttttggcta ttgatttggc cgattctcag
    64681 agtccacaag atatgattgg ttcctgcttc cacctgaccc acagctgata actcataggc
    64741 gcattctgat tttggagata aaattcgatc caaaaacttg ccacccaagg ttaccttggt
    64801 ttcaacagcc tctttttctt catgaccttc ttccagactc cacaagattt cctgaccacg
    64861 ctcatcattt ttcagaaaat gctctagcgc tgccaaatgc acacagtaac ccctcttttg
    64921 aaaaaaatca caggcacaaa aaaccaaatc atcctctaaa ctatagcgca gttcttcttc
    64981 tgcaacgcga gcgtagagcc gattgttctt ttccttgatg atatcaacct taccagtttc
    65041 ataaagggca acaccttcga tacgaatttt ccccggaatc aatttagcca tattttcacc
    65101 tttaccttat ctttttatta taccatattt tcgcctatga aaatagcctt ctaggaagac
    65161 ttttctccta gaaggctgga tttttaacgt ttggcaaaag tagccacaat ccgctgacag
    65221 acttcttgca acagagattt gggcatagct atattgatgc gggcatggag acttccttcc
    65281 tctccaaaat ccaaaccacg gttgaggata accttggctt catttctcaa caactcttgc
    65341 aatgtttcat cagtcaggtc ataagctgaa aagtcaagcc aaatcaagta ggtaccttgc
    65401 ggtttcatga ccttgatttt agtctctttt ccaaatagat ccaccacata attgatgtgg
    65461 tcttcaaaga cttgcttgag ttcctctagc caatctttac cgtatcgata ggcagcttct
    65521 gtcgccaaat aacccaagcc tgaaatttca tgctgattat tggccaacag gcgtttctgg
    65581 aaagctagtc tcaacttagg attttcaatg actgcatagg aattttttgt tccagcaata
    65641 ttaaatgttt tagtggcact actcaagacg atagcaaaat ttttgaaggc aggattgatg
    65701 gtattgaaag actggtgttt gtgaccaaag agggtcaaat cttggtgaat ctcatccgaa
    65761 actaacaaaa caccgtgttt ttggcagagt tggccaatct tctccaacac ttctttttcc
    65821 caaacacgtc caccaggatt gtgagggttg caaagaatat agagtttaac ctcctcttcc
    65881 accaaatcct tttcaagttg gtcaaagtca atctcaaaca gaccatcctt ttccactaag
    65941 gaattagtaa tcaatctacg attattcaac ttgacactgc gagcaaaggg tgggtagaca
    66001 ggcgtgttaa ttaaaaccgc ctcgccttct tttgtaaagg tttgaatagc tgttgagatg
    66061 gctggtacca caccctcgat aaagacaaga gcctctttgt caaagttgta accgtattgt
    66121 gtagcttccc acttttgaac ttccttaatt aagtcttcac tggcataggt ataaccataa
    66181 accagttggt ctgcgtaagt ttgcacggct tggcggattt caggcaagac cacaaagtcc
    66241 atatccgcta tccaagctgg tagaacttca ctatccgttt ctgtttcttt ccatttatag
    66301 gtatggtgcc ctaaacggtt gggcaggctt gtaaaatcat attttcccat ctttgtctta
    66361 tccttctaag gcttggcgca aatctgcaat caaatctcta gcatcctcaa tcccaataga
    66421 caaacgcaag aggtcatctg tcaaaccata agaatggcgt acttctgctg gaatatcagc
    66481 atgagtttga gtcgttggat aagtaataag actttccact ccgcccaaac tttccgcaaa
    66541 agagaagacc ttgagactgt tcaaaatatg aggaatgcgt gtttcatcgg ctactttaaa
    66601 ggaaatcatg cctccacgac cagtgtagag aacttcctta actgctggag aatccttcaa
    66661 aaaggcaacc acttcttggg cgttagctgt tgagcgctcc atacgaagag acaaggtctt
    66721 gagaccacga agcaactggt agctgtcaaa tggagacaag actgcccctg ttgtattgag
    66781 attgtaaaaa agcttctcgt atagttctaa actattggtc acaaccactc cagccaagac
    66841 atcattgtgg cctgctagat acttggttgc tgaatggaga acgatatctg ctccatcttc
    66901 aatcggacgt tggtagatag ggctatagaa ggtattgtcc accaccactt tggcaccctt
    66961 agcatgagcc aattttgcta gtttttcgat atcaaattcc aacatcaagg gattggttgg
    67021 ggtttcgata tagagaacat ccacatcctt ttctaactcg gcaatcaact cttcttctgt
    67081 attggcatag gtaaaatgga aacgaccttc ctgctccact tggttaaacc agcgaaaaga
    67141 accgccgtaa agatcacgca ctgccaagac cttacttcct actggaaagc cgctaaaggc
    67201 cagtacaata gctgacatcc ctgagctagt cgctaaggca tagtctgctg actcaatagt
    67261 cgccaagact tcctcagcct tactacgagt tggattttta gtgcgcgtat agtcaaaccc
    67321 agtagatcga ccaaactctg gatgctgata ggtcgttgaa aaatgaagcg gtgtcaccaa
    67381 tgcacctgtc gcttcatctg acttaatacc cgcctgggcc aaaattgtgt taatgtgtaa
    67441 ttccttgctc atacaatacc tccaaatcta tagtaactat tgtaccactt attttcatca
    67501 actcattttc ttcttttcaa gagctagtta tagtttcaaa ctatataaaa agggagtttt
    67561 tcctgctccc tttaatagac tataaaatgg tgaatctcaa aagacacttc actctatcat
    67621 ttgctcctgc acaaaacgag cataacgctc atgattttcc agtagttcct tatgagttcc
    67681 tgagccagtg attttcccct cctctaagaa gaaaatacaa tccacatctt ttaccgttga
    67741 caaactatgc gctataatca caaccgtctt ctcctttaat acagaataga ggctactgat
    67801 aatcgcatac tcagaatccg catcaagatt agcagtagct tcatcaaata taagaatttc
    67861 agcatctttt aagtaggttc tagctatttg aagtctttgg ctttgccccc ctgacaagaa
    67921 tcgtccgcgt tcaccaactt cagtatctag tccctctttc atggagcgaa tctcatcacc
    67981 tagtgatact aagtctagca ctttcatcaa ttcatcatca gttactaagc gattcaaacc
    68041 gagacaaaga ttgtcacgaa tactgccaga taagactgca ttattttgtg aaacccaagc
    68101 gattttactt ctccattctt ttaagttaaa atcatatata cttgattgct ccattagaat
    68161 atctcctgaa agcggtttat aaaaccgctc taacaaacgc acaatcgttg attttcctga
    68221 tccagatggt ccaacaaaag caatttttgc cccttgaaaa ttgaacaagt aatatccttt
    68281 aagacaggtc gattttcatc ataaccaaaa tagacatggt taaaattcaa cccttgtcct
    68341 gataccgatt ttcctccctc aaatttttct ttaggaactg caagcaagtt atccagtgca
    68401 actgaagatc ccttgctcct agaataaaca gttacaaaat tagctatatt actaatagga
    68461 ttaagtaatt gaaagaggta aatcaaaaac gaaaccaagg ttcccacaga tatatatcct
    68521 gcgctgaccc gataaccccc ataggttagc atcacagcta tagtcgcaaa gataaataag
    68581 agagcaaacg gggtctcaaa agaagtaacc ctatctgatt tcagtgaatt gttttgtacc
    68641 ctttcaatac aattatccaa aacatcctgt acacttttct ctgcttggtt agtcttaatt
    68701 aattcatgtt cttgaatctt ttcagtcaat tgccctgtta aatttcctcc tgtaaacgac
    68761 gactatactt ttcactgata ttggaaaggg gcaagataat aaacatcata caaggaagag
    68821 tgatgaataa aagtagagaa agattccaat caagactaaa taagactaca atggaaccaa
    68881 gtaccataac taaactcaga ataatatttg ggaaagtcgt aattaaaaac tcacgaatga
    68941 cactcatgtc attgacaatg gcagaagtca actccccact ttggctctta tcaaagaagg
    69001 atttctctgc ataaatcaac ccctctatca cttttttcct gatttttgct atcttttttt
    69061 cacccgattg actaaacaga tagtaaccaa tagaagaaaa caaggcttga ccaatacgat
    69121 tgaaatactt tggaacctat attttcaata gaactcccat ctattaaacc ctttaagata
    69181 aggggaagca acaaagcaag tagactagac agaacaagta agaaactccc cataatcacc
    69241 ttagtatcta ctcttaataa ttttaatttc ataaatactc cttataatat ttcaacggat
    69301 aaagtcggga ataactcaat ttgaggataa aatctaataa atcttcctat aacaaaacgc
    69361 ataacatcta ggattttata tacctgatat tatgcgtttt taagcacaaa gacttcttac
    69421 acaaacttat ctacaattag attttatttg acatgttttg ccaattcttc ttgggctttt
    69481 ttattggatt cttctttttc tttcaaccat ttttctctgg cttttgcata ttcgtctgtt
    69541 gtgacaatct tatcttgtac tttgaggtat ttatatgatt caaccccttt tgtaccggtt
    69601 aaaccatagg cagcagcaaa tggtacggtt cttctcaatg atggtgttcc cccacgcgaa
    69661 acacttggaa gaactaaaga actatcaatc aaccaagctt gaatatcagc atatttctca
    69721 taacgtttgg ctggatcttg ctctttatta gcttcttcca acatttgagt atagacatcc
    69781 agtccaactg ccttagcctt gtcattggct tctccaggct ctagtccaag attttgtaga
    69841 actcctccac tattagtatt aaaaatatcg agataggttg acgggtcttg ataatcaggt
    69901 ccccaaccgc catgatataa atcatagtct ttctgagcag ctgtttgagc aaagtagcct
    69961 gaactgtcaa actcatctga tgttaattgc tgaatgtcaa tcactacatt atcagaacct
    70021 aaaacagatt caattgattg tttgatagaa ctaactcctt gtatgcctac tttatctgtt
    70081 acttccacag tcttatccaa gtggattggg aattgaacac cctttgcttc gagttctttc
    70141 ttagcttccg caaacttagc cttggctttc tcaggattgt agtaagggtc ttgaccatcc
    70201 gcaaagttga taccttgcca ttccttacca tagttgacca tcttagaggc tacaacttca
    70261 ccaaagtctt ttcccttgat actgacaaag tttggaggaa ccactaggtt acgcaaaatc
    70321 tttgttgcac cttctttccc ttcagactga gccccataag atgttctgtc aaaagcaaaa
    70381 ttgatagcct gacggaagtt tttattgaga actgcttcct gggtcgattt cttttcaatg
    70441 tcacttgttt tagaagtata attgtaagac ttcctatcta ggttaaaatt aaagaaatat
    70501 gaagttgaat tttgcatact atagatgata ttgtttttgt atttttcttt aatcccttca
    70561 tagctggagc tgttaggaaa aagacgagcc gtagtataag caccagctgt aaaattacgt
    70621 tccagtgatt cttggtcgct accatcatag taggtcaatt tcacatcgtc tacaaagaca
    70681 ttcttagcat cccagtaatt agggtttttc ttatattcaa tagcagattt tgagacaagt
    70741 gctttcatca agaaaggtcc attgtacaaa atactagatg gatccgcctt cccaaaatca
    70801 tccccttttg atttcaggaa atctgcatta acaggaaaaa gtatcgttgc aagtgttttt
    70861 gaattccagt aaagttctgg tttaaccaaa gtatattgaa ccgtttggtc atcaagtgcc
    70921 ttgacaccga cagttgaaaa gtcgcttgtt ttaccagtga tatagtcatc caaaccagca
    70981 acagagtcct gcactagata caaagcttct gattttttat cagctgcata ttgcaaacct
    71041 gtcacaaaat cctgggcagt tacaggcgca tattcttctc cctcagaagt aaaccacttg
    71101 gcatccttac gaagtttgta ggtataggtc aaaccgtcct gagaaacagt ccaatcctct
    71161 gctaatgatg gaataatatt cccatattgg tcattttcta ataacccgtc taccaaattt
    71221 gcaacaatat cggatgttgc tgcgcggttt tctgctagat agttcaaact agatggatca
    71281 cttgaataaa catagttata ggtttttgat gcagtgctag aatttccaca cgcgctcaat
    71341 aaaactcctg tacccaggac aagacctgcc aaggttagat atttgctctt agactttttc
    71401 atttccagaa cctcctgctt taaatatttg ttttattata caactcttgg tttgtttagt
    71461 caattgtttt ttccaaaaaa tcaaaaaaac ctcattttct atttttgaaa gataatttta
    71521 gctattttga cgctaaaaaa ggaataagtt tcaagttctc ctaaaagatt gttcaactta
    71581 ttccttttat ttataaatgc taccttacga aagcttaaat tttgctcgca ggcgatctgc
    71641 ttgggctttt cctattacct tatctaataa ataggtacgc agactcataa ccatataaag
    71701 tccacccccc atggcaccga caagagctac ataaaagaag ctccacaaac gtccacttgg
    71761 ttggaagaaa aatcctaaca gccactggat ggttcctatt aacagaaaca tgactagggt
    71821 cagcaaactg attaaaatgg ttcgcttcaa aatcaccttg cgcttgacac cagttacttt
    71881 acaaatatcc cgatacatca agacgttagg aatgatgaga gcaatggttg ttgaaatcaa
    71941 aggaccataa ctgtggaaga gggcgatggt aggtagttgc aagactagct tggcaataga
    72001 accatagata aaatagagaa cggccttgcg gttgcggaac atggcctgaa gcattggaga
    72061 caagaccatg tacaagccta aaataataga ctgcaaaact gcaaagacaa ataagcccag
    72121 agccaaacta tctggcttac catagaagac cgtataaaga ggttctccta ccataaccac
    72181 tccaaccgtt gctggtagca agaacataaa gagtagggtg agactgtcct gaacgagacg
    72241 agaagccgct ttcaagtccc ccttgacata gttttccgtc aaaagtggca aaccaacact
    72301 cccaatcgaa acccctacag aaatcaaaat catcgtgatt ttattaggat tggctgagaa
    72361 ataagaaaac atgacaacca agtcctcatt gctgtagttg gtaaaccagc tcatactatt
    72421 gataaaggtc agctgatcca aaatctggaa gagctggatg gcagaccctg tcaggataaa
    72481 aggaatggct tccttaatgg tatcaaccaa gagacgctta ctgttaatct tatctcctgt
    72541 ttcaaagact cttttgagtg aaccttcttg ggcaaggaaa taaatcaaga ctgcaaaact
    72601 ggctaccata ccgacaaagg cagcaaaggt tgattgggta acggctgcta gataatctcc
    72661 tgaaccgagc ttcataatga taaaggttgc taggagcatc cagataacac gaatgacctg
    72721 ctcagcaatt tggctcatgg catagggttt gaggttattc atcccttgga aaaatcctcg
    72781 gataacactc atagacggga aaatcaagac tccccaagcc aagctttgca tgattgggat
    72841 caagtcttta cccacgccag acaagtctgc tagccaagga gcaaagacat acaagactaa
    72901 agcaaaaacc aggcctagtc ctgtcataaa gcctaagaag ctccgaatca gggcaaagct
    72961 atgctcttct tctcgcatgg tattatactt ggcaacttgc ttggccaccg caactggaat
    73021 ccccgctgtt gaaaccaaca agaaccaagc atagatattg taacccatgg taaagagacc
    73081 atttgcctta gctgcataag cccccatcca gatgtaccaa gggataatgt aaacagcccc
    73141 gagtaggcga ctgataaagt tactagccgt tagccaagca gtcccccgta acatctgggc
    73201 ctgctggtga ttgttttcgt gcgacataga ttcctcattc aattttgata gctaggaaaa
    73261 actcctcatc ttccattata aacttttcct tgttacttgt aaatttacga ctttcggttt
    73321 acaatagaaa gtatgattaa gattgaaacc gtattagata ttttaaagaa agatggcctt
    73381 tttcgcgaaa ttattgacca aggtcattac cactacaact acagcaaagt tatttttgat
    73441 agcatcagct acgacagccg aaaagtaaca gaagacactc ttttttttgc aaaaggcgct
    73501 gcctttaaaa aagaatacct tctttctgct ataacacaag gtttagcttg gtatgtagct
    73561 gaaaaggact acgaagtcga tatccctgtc atcattgtga acgatataaa gaaagccatg
    73621 agtttgattg ccatggagtt ctatggtaat ccacaagaga aactcaaact ccttgccttt
    73681 actggtacta agggtaagac aacagcaacc tatttcgcct ataacatctt atctcaaggg
    73741 catatacctg ctatgttgtc gaccatgaac acaactcttg atggcgagac tttctttaag
    73801 tcagcgttga caacccctga gagtattgac ctctttgaca tgatgaatca ggctgtgcaa
    73861 aatgaccgta cccacctcat catggaagtc tccagtcaat cctatctggt caaacgtgtc
    73921 tatggtctaa cctttgatgt gggagttttc ctcaatatca gcccagacca tatcggcccg
    73981 attgaacacc ctagctttga agactatttc taccacaagc gtctcttgat ggaaaatagc
    74041 cgagcagtca tcattaacag tgacatggac cacttctcag tcttgaaaga acaggttgaa
    74101 gatcaagacc atgatttcta tggtagccaa tttgataacc aaatcgagaa ttccaaagcc
    74161 tttagctttt cagctacggg taaactcgct ggagattatg atatccaact cattggcaac
    74221 ttcaaccaag aaaatgcagt tgctgctgga cttgcttgtc tccgtctcgg agcaagtctt
    74281 gaggacatca aaaaaggcat cgctgcaacc cgcgttcctg gtcgtatgga agtcctcact
    74341 cagaaaaatg gagccaaggt cttcatcgac tatgcccaca atggggatag tctgaaaaaa
    74401 ctcatcaatg tggttgaaac tcatcaaacc ggaaagattg ctctggttct gggatcaaca
    74461 ggaaacaagg gagaaagtcg tcgtaaggac tttggcctcc tcctcaatca acaccctgag
    74521 attcaagtct ttctgactgc tgatgaccct aactatgaag acccaatggc cattgcagat
    74581 gaaattagta gctacatcaa tcatcctgtt gaaaagattg cggatcgcca agaagccatc
    74641 aaggcggcaa tggctatcac aaatcacgaa ttagatgcag ttattattgc gggtaaggga
    74701 gccgattgtt accaaatcat ccagggcaag aaagaatcct acccaggaga tacagccgtc
    74761 gcagaaaatt atttataaga atagaaaaaa tcaagggaaa atgaaaccct tgattttttc
    74821 ctattattta gtaaaaacgt ttttcaaacc ttcaacggca ccttctacag caccttttgc
    74881 gtcttctaca acgtcttttg ccttagcaac tgttttttca acagctcctt caagttctgt
    74941 cttgctgtct ccagtaactt taccaaaacc ttctttgaca gcgcctgttg cttgttccaa
    75001 tttgttttca agtgacatat gattgtctcc tttagtttat tccgatatgt gttaccggtt
    75061 acatatagtt tataccgaat actaaggaaa gtcaaacaat gtgctcagaa acaagaaatc
    75121 acgctagtag aaagttaatc tctccttatc aaaatcgata gccaactcat actttccatc
    75181 ttcattttgc acaatatagc ctaagacaac taaactgtcc acaaagatat cacggcattt
    75241 ctgcataagc tggtcttttt tgagaaattt caacaaaaaa gccgtcatat acttgagggc
    75301 atactcagga ttaacatctc ctaaaatgtc atagagctcc tgctgttttt ctgtcaaagg
    75361 atactgctgt ttgaccttgt aaaaataatt ggacagggtc attttaatgc gcgcaaagtc
    75421 cgtcttttca actaaaatag ctgcattgat ttgattgcgc aattccgtct caaaactctg
    75481 ctccaacaag gcttgataga ccggactagc ttctctgaca aaaatctctt gatccagttc
    75541 aagactatca agtgattcaa gcgtaggaaa attgaggtaa tagcgcttat tttcacgtag
    75601 aatcaagctt gcctttatat actcttccat gagtttatca actgcaacat ctggaaattg
    75661 agccttaatt ccccgtagaa tcacatcctc atactggtcc agatagcgga tcaattctcc
    75721 aaaaatggct gtctcgtcaa acgagatgga ttaaaaatct gaatcatgaa gattcctttc
    75781 tttacattgt aacagaggcg atgctgccaa ctgactagga ttggtaccag cttggttcag
    75841 aggaacaggc agacctagtt ctatgtaata ctccctccaa aaatcactaa tctcctgctc
    75901 cccatctcca aatttcatag gaatagtttc aaaaataggt agaatttctg ctatatactg
    75961 ggagcagtag aaaccagctg catctggata gaaagaagca ttgtagggtg ctccaagatg
    76021 tttgcaagct ctttccttca ccgactggat atccatttct ggataaacat agaggtcgta
    76081 caaatgattg gactcaaaga agtctgccgg ttcttgacag acaacaccag cctgtccact
    76141 agcatgataa atcatcccat ccaaataaat ggcaacatgg ctatagttac ctgtggaagt
    76201 ctggatggcc tgtcccatgt ctgacccatc tctcacaaaa atcaaatcgc cattttctaa
    76261 catacttcac tcctagaaaa aaaggagccg aaactccttt cgatataagg caaactagac
    76321 tttccgattt tgagctgaca cccaaaatct tacgcattaa aactttcagt caattgaggt
    76381 accacttgtt tcttacgtga aacggcacca gcaaggaagg catgattgtt ttcaagtttg
    76441 aagttgaaag ccgcttcgac cttgtccata ttggcaccaa gagccaagat ttctgagttt
    76501 gagttgacga tatctgtaat catcaagaca aagtcagagt agccgtttga ttcgttggca
    76561 gcttgcattg cagcttcaat ttctgcttgg cgttccaaaa cttcagcgat gtcaactgtg
    76621 ttcacttggg caacacggac attatttccg ttgagttcaa aagtcttagc atcgatatca
    76681 atcaattctt cagcagattt gctagccaag ttggtaccag ctttcaacat tgccaaacca
    76741 tattcttcca agttcacacc agccaattca gccaattcag gagcaatgat tttatctgtt
    76801 gggtgtgttg ttggtgattt caaaagaagg gtatctgaaa tcaaacctga aagcatcaaa
    76861 cctgcaatct ctttaggcac agctacacca tgttctttga acatacggta aacgattgaa
    76921 gacgctgatc caactggctc caaacgcatg taaagtgggc ttgcagtttc aaagttagcc
    76981 acacggtggt ggtctacaac accataaact tctacttcag cgatatctga tacagattgt
    77041 tggaattcat tgtggtcagt caagataact tgctctgccc cctctgcttt ggcagaagtg
    77101 ataacacgtg gtgcttccac accaaaatag ttcaagacaa aggctgtttc ttcatttgga
    77161 gttccaaggg caacagcttc cgtatccaaa ccgtaagctt cttttgcaag gtaggcaaaa
    77221 gctacagatg acccgatggc atctgagtct ggattttggt gaccaaatac tagaatcttg
    77281 gacatgataa tacctcattt tgtttatttt ctttattgta gcattttttt cgctttttga
    77341 caaaggtaag aggagtcaaa ggactcccca cgattcttca aaataactga gtaaatttct
    77401 atcttgattt tcagataaaa attcttcctt ctgtggcctc ttcttacgct tgagaagagc
    77461 ttcagccgac atggcttctt ccttactggc aaaaccttga gcatagataa gtttgactgg
    77521 caagcgtgct cgtgtatatt tggctccctt cccactattg tggatagcga ggcgtcttct
    77581 cacatcagtc gtatagccta tatagtagga tccatcacga cactccagaa cgtacatata
    77641 agccttatga tccataataa atctcttcga tttcgggcgt ataagagcca tcatcattgt
    77701 ggacaatcaa aggaggtaag accttaaagc cacttgttga gccatccttg atcgcctcaa
    77761 tcaaaagcat attggcttcc ttttctcttt ttggataaac aaactgcagg cgcttagggg
    77821 ctagattatg tcgttttaac gtatccaaaa tatccagaag tcgatcagga cgatgaacca
    77881 tggccaaacg cccattagac ttgagaatac tctgggcact atgacagatt tcttccaaat
    77941 tagtcgtgat ttcgtgtcga gccaagagat aatgttcact ctcgttcaga ttagaataag
    78001 gatccacctt gaaatagggt ggattacaca aaatcatatc caccttactc ccctgaatgt
    78061 gagcaggcat atttttcaaa tcatcgcaga tgacctgcat ttgctcctct aatccattca
    78121 aacggacaga gcgttcagcc atatccgcca aacgctcctg aatctcaaca gacaatatct
    78181 gtgcttgagt acgagtgcta gcaaaaagcc ccactgctcc attcccagca cagaaatcca
    78241 caatcaaccc cttcttagga aaacgtggaa atcgtgataa gagaacacta tccaccgaat
    78301 agctaaaaac ctctctattt tgaatgattt tgatatctgt cgaaaagagc tggttaatgc
    78361 gctctcctga ttttaataat tgttcttctt ccatggtcct attatagcaa atttatatta
    78421 acattacaaa aaatataaaa ctctaaacta cttcttcttt tttaaatggt gcagggcttc
    78481 tccagtccag attggtagca ttcgtcgaaa gggagcaaaa ccgtagttaa agcggtcgct
    78541 tgaaaagcgt ctccgtctag gaaactggta cttttcttcc tccaaagtgc ggatagaaag
    78601 actggctttc cctgtaaatt catctaaatc cactacctga acttgaacct cttcatcgac
    78661 tttcaaggtt tcatgaatat tttcaataaa tcctgtccga atctctgaaa tgtgaatcag
    78721 ccccgtatca cccgtctcta actcaacaaa ggcaccgtag ggctgaatcc ctgtaatacg
    78781 cccctttagc ttatcaccga ttttcatctt agtcctcgat ttcaatagtt tcaattacaa
    78841 catcttcaac tggcttgtcc atagctcctg tctcaacagc agcaatgaca tccaagacag
    78901 cgtaagatgc ttcatcagct aactgaccaa aaaccgtgtg acggcggtct aggtgaggtg
    78961 tcccaccttg attggcatag atttctgcaa tcggttctgg ccaaccacca cgagtaattt
    79021 ctttcttaga ataaggtagg tgttggtttt gcacgataaa gaactggctg ccgttggtat
    79081 ttggaccagc atttgccatg gaaagagcac cacggatatt gtaaagctct tctgagaatt
    79141 catcctcaaa agattcgccg tagattgact cgccacccat accagttcca gttgggtctc
    79201 caccttggat cataaagtcc ttgataatac ggtggaaaat gacaccatca tagtagccat
    79261 cttttgaaag agatacaaag ttagccactg ttttaggagc atgttaaggg aaaagcttga
    79321 tacgtaagtc tccgtgattg gtcttaatag tcgcaagagg accttctact gtttcaatgt
    79381 ctacttgtgg aaaatgcaat tctttttcta ccataccaaa tacttctaag gcatcaaaaa
    79441 tgccatcttc ttctaatgtt tttgtaatat aatctgcttt ttctttgatt ttatcatgag
    79501 aaattcccat ggcaacgctg attccagcat aatcaaagag ttccaagtca ttgagaccat
    79561 ctccaaaaac catgaccttc tctggtttca agccaaggtg ttccacaacc ttttccaccc
    79621 ccgtcgcttt ggagcctgaa atcggcacaa tatcagacga atgttgatgc caacgaacca
    79681 tgcgaagttt gtctgagaga ctgtcaggca agtgcaagtc atctccctta tcttcaaaag
    79741 tccacatctg atagatatct tctttttcat ggaaatcggg atctacatct aagtcgggat
    79801 aaattggatt gatagcttca ctcatcatat cggtgcgagt cgacaacttg gcatcatgac
    79861 tcccaaccaa gccatactca attccttctt gcttagccca agagatatac tcctcaacat
    79921 atgacttttc aatctgatgc tgataaatga cctgaccttt tttatcttcg atataagccc
    79981 cattcaaagt tacaaaaaag tcaggcttga gatcacgaat ctctggaaca acaccaaaaa
    80041 tgccacgtcc agaggcgatt cctgttaaaa ttcctttttc acgcaactgt ttaaaaacag
    80101 tgggaattgt agttggaata aaccctgtct ttgaattccg caatgtatca tcaatatcaa
    80161 aaaagacaat cttgatcttc tttgccttgt atcttaattt cgcgtccatc tcactacctc
    80221 tttcaatcta actctttcca ttatatcata aagtaggcaa atcccctatt ttcaaaaagt
    80281 ttatcatttt tattttaatt tcttggatga gaaaagagac atatttatga aaaagctcca
    80341 tcgtgctttt aatgtgttct cttgttttca aactcgtaaa aagggagcca ctgagcctaa
    80401 ctcgctctct catttcaaag cttgtgaaaa aagacccgtt ggggtcttaa ttcgctttct
    80461 tgttttcaag ctcatgaaaa agagacccaa ccgggtcttt tctttaatct tcgtttacga
    80521 aaggcatcaa agccattacg cgagcgcgtt tgatagctgt tgttacttta cgttggtttt
    80581 tagctgaagt tcctgttaca cgacgaggaa ggattttccc acgttctgaa acgaaacggc
    80641 taagaagctc agtatctttg taatcaacat attcaatttt gtttgctgcg atgtaatcaa
    80701 cttttttacg gcgtttgaat ccgccacgac gttgttgagc catgtttttt ctcctttata
    80761 agtttagttg tccattagaa tggtaaatca tcatctgaaa tatccaatgg gtttgttgct
    80821 ccaaatggat tttcattacg tgaaaagtct ggtactgaat ttgtaggtgc tgaatagttt
    80881 gcagttggtg cagagtaagc tccacctgtg tgaccctcac gcacactacg gctttccaac
    80941 atttggaaat tctcagccac gacctctgtc acgtagacac gttgtccttg ctggttatcg
    81001 taactacgag tctggatacg acctgtcacc ccgataagtg agcctttttt agcccagtta
    81061 gcaagatttt cagcctgttg gcgccacata acgacattga taaaatcagc ctcacgttca
    81121 ccattttgac tcttaaatgt acggtttact gcaagagtaa aagtcgcaac tgctacattt
    81181 gatggggtat aacgcaactc agcgtcacgt gtcatacgcc ctacaagtac aacattgtta
    81241 atcatagttt accttcttac gcgtcaattt tgacgatcat gtgacgaaga atgtcagcgt
    81301 tgatttttga aagacggtca aactctttaa gagctgcatc gtcatttgct tcaacgttaa
    81361 cgatgtggta aagtccttca cggaaatctt ggatttcgta tgcaagacga cgtttttccc
    81421 aagtttttga ttcaacaaca gttgcaccgt tgtcagtcaa aatagagtca aaacgtgcta
    81481 ccaaagcgtt tttagcttct tcttcaatgt ttggacgaat gatataaaga atttcgtatt
    81541 tagccattga tatgttcctc cttttggtct aatgacccca agactttgca aggggtaagt
    81601 gaggttcgct cacaataaac tattatacta gaaaaaattt ttttacgcaa gtaaaaacac
    81661 tagaattcga aaaaacgcca catgggcgtt ttcctgttct tatggtttga tacggtgcaa
    81721 catacgtggg aatggaatag cttcacggat atgttttgtt cctgctgcga aggttaccat
    81781 acgttcgata ccgataccaa atcctccgtg tggaactgta ccgtatttac gaaggtcaag
    81841 gtagaattca tattctgtac gatccatgcc aagttcatcc atcttagcga caagggcatc
    81901 gtaatcttcc tcacgcatag acccaccgat aatttctcca tagccttctg gagcaagcaa
    81961 gtctgcacaa agcacgcgct ctggatttcc aggaactggt ttcatgtaga aggccttgat
    82021 ggctgctgga tagttcatga caaatgttgg cacaccaaag tggtttgaaa tccaagtttc
    82081 gtgtggtgac ccaaagtcat caccatgctc aagatgctcg tagtcagcat cttcatcatt
    82141 ttcatgctct tgcaagaggt caatggcttg atcataagtg atacgtttga atggctctgc
    82201 aatgtagcgt ttcaagagtt ctgtatcacg ttccaaggtt tccaaggctt gaggcgcgcg
    82261 gtcaagaaca ccttgtagaa gagctttcac ataagcttct tgcaagtcaa gcgactcatc
    82321 atgtgtcaag tatgagtact cagcatccat catccagaac tcagtcaagt gacggcgtgt
    82381 ttttgatttt tcagcacgga aaactggacc aaagtcaaag acacgaccaa gagccatagc
    82441 ccctgcttct aggtaaagct gacctgattg gctcaagtag gctggcgttc cgaagtagtc
    82501 agtttcaaag agttctgtag aatcttctgc cgcatttcct gaaagaattg ggctgtcaaa
    82561 cttcataaaa ccgttcttgt caaagaactc ataagttgca tatataatag cgttacggat
    82621 ttgcaacaca gctacttgct tacgagagcg tagccacaag tgacggttat ccatcaaaaa
    82681 gtctgttccg tgttcttttg gtgtgattgg gtagtcttga gattcaccga tcacttcgat
    82741 gtctgtgatg tccaactcat agccaaattt agaacgttcg tcctctttga caatacctgt
    82801 cacataaaca gacgtttctt ggctcaaacg tttgataaca tcaaacttct caagtcccac
    82861 ttcttcacca aatttttcga caaagtttgg tttaaaagcc acaccttgaa agaaggctgt
    82921 tccatcacgc aattgtaaga aagcgatttt tccttttcct gatttgttgg caacccaagc
    82981 gccaatcgtc acttcctgac caacatagtc ttttacgtca ataatcgtta cacgttttgt
    83041 cattattttt ccttttcttt tttattcttt atggcaaacc acctctatat tgttcccatc
    83101 caggtcaatc ataaaagcag catagtaaat cggatgctca cttcgataac caggagcccc
    83161 attgtctcgc ccacctgcct ctaagccagc ctcataacaa gcctgaactt cttccttatt
    83221 ttctgctaaa aaagcaaaat gaacaggatc ttgtgttccc tgagtcagcc aaaaatcacc
    83281 accaggatga gggctgttcg gggatagaaa actaattaga gaacttgtct taaaagtcat
    83341 tttatagtcc aaaggagcga gaaaactcct ataaaatcct tctgaaattt gtaaatcctt
    83401 taccttaatc tcaaaatgat caatcattct cactacccat aaatgctttc aagcgttcga
    83461 ctgcttcttt aagcgtgtct aggtctgtcg catagctgag gcggacattt tctgatgctc
    83521 caaatccagc tcctgttacc aaggccactt cggcttcttc taagatagca gttgtaaagt
    83581 ctgtcacatc cgtgtaacct ttcatctcca tggccttttt gacatttggg aagagataga
    83641 aggccccttg tggtttgacc acttcaaatc ctggcacctc tgcaaggagg ggatagatgg
    83701 tattaagacg ttcctcaaag gcctgacgca tgctttctac agtatcttgc tcacctgata
    83761 gagcctcaac tgctgcatat tgggctactg ctgacggatt cgaagttgtt tgacctgcaa
    83821 tcttggacat ggcagcgata atgtctgctt ctccaacggc ataaccaatc cgccaaccag
    83881 tcatggcata ggttttagac acaccattga tgaccactgt ttgcttgcga atcgcttccg
    83941 ataggctaga aatcggtgtg aactcatgac cattataaac caagcggcca tagatatcat
    84001 ctgctaggat gagaatatca ttttctacag cccagtttcc aattgccaag agttcctcac
    84061 gggtgtaaat catacctgtg ggattagatg gcgaattcag caccaaaacc ttggtcttgt
    84121 cagtgcgagc tgcttctaac tgctctacgg tcaccttaaa gtgattgtct tccttagcag
    84181 aaacaaagac gggaacgcct tctgccatct tgacctgatc tccatagcta acccagtatg
    84241 gggttgggat gatgacttca tcacctggat tgaccacagc cataaagaag gtatagagag
    84301 aatatttggc tcccgcagcg actgtcactt gatttgacgc tacagaatag ccgtaaaagc
    84361 gctcaaagta gctattgacc accgccttaa gctctggcag acctgaggtt actgtataaa
    84421 aagaagcacg cccatctcga atcgatgcaa tggcggcatc ttggatattt ttgggagtag
    84481 tgaaatctgg ctcacccaag gttagagaca aaatatctct accctcagcc ttcagtgctt
    84541 tggcacgggc tccagcagcc aaagtcacac tttcttccat ttctaaaaca cggttggata
    84601 gtttcatagg ccctccttat tgaccaatgc tcctgtttca aaatctacta gataaaaatc
    84661 agatcctgac ttaacttccc agattggctt gtcttgataa cgaccaaagg ttatcttgtc
    84721 aatctcgcca gctccctttt ccttagaaac cgtttctgct ttttcttgtg aaataccctg
    84781 atttagctga taaacgtaaa tcttatggtc atctttacca atcaggacag caagcgcttc
    84841 ttgctgttta ttacgaccaa gaacgctgta ataagattcc aagccattgt ataaatcaac
    84901 ctgatcagcc ttctctaatc ctgcatactg ctgagctaat ttttctcctt cacttttagc
    84961 tgtttgatag ggtttcatgc taagagaaac catatacaga aaggaagcac tgataaccac
    85021 aaacaaaatc gtcatcccta gaccatactg ccacagtaga ttattttttg ctttgttttg
    85081 tctttttttc actcgtctat tttaccatct attaagcttt attacaagtg aatataagaa
    85141 tactcttcga aaatctcttc aaaccacgtc agctttatct gcaacctcaa agctgtgctt
    85201 tgagcaacca attctatttc tcccttcaaa caaaaccgat tttgaaagtg aaacagttct
    85261 tactttttca gtcacaaatg actagagttt ccgaaatttt acaattttca aataaactct
    85321 gagcaaattt cttgcaagtt cttttgtttt gttgtaatat attttataac aacgagagag
    85381 ttctcgaaat tttaagaaaa aggagacaca tcatgtctaa aaaagtatta tttatcgtcg
    85441 gatcactacg tcaaggttct ttcaaccacc aaatggcgct cgaagctgag aaagctcttg
    85501 ctggtaaagc ggaagttagc taccttgatt attcagccct tcctctcttc agccaagatt
    85561 tggaagttcc aacatatcca gctgtagctg ctgctcgtga agcagttctc gttgcggatg
    85621 ctatctggat tttctctcca gtctacaact tctctatccc tggtacagtg aaaaacttgc
    85681 ttgactggct atctcgtgcc cttgacttgt ctgatacacg tggcgtttct gcccttcaag
    85741 acaagtttgt cacagtatca tctgtagcca atgcagggca cgatcaactt ttcgctatct
    85801 acaaagacct cttgccattt atccgtacac aaggcgttgg tgatttcact gctgcacgtg
    85861 ttaatgactc tgcctgggca gacggaaaat tggttcttga agaaacagtc ctaaactcac
    85921 ttgaaaaaca agctcaagac ttggtcgaag ctatcaagta actaacactc aataaaaatc
    85981 aaaaagcaaa ctaggaagct agccgcaagc tgctcaaagc actgctttga ggttgtagat
    86041 agaactgacg aagtcagtga catatatacg gtaaggcgac gctgacgtgg cttgaagaga
    86101 ttttcgaaga gtataacata ataaaagcga acaagactac ttttctgaca ctcagaagac
    86161 tagcttgttc gctttttttc tcatttataa actaaaacag ctgatgatac atattgccaa
    86221 atacaaaata tctgatttct atacttcctt tacaaaaacc aatccctgtg gttgtgacaa
    86281 atcttctcgg taaacaaatc cagcaaagtt caagcgacgt gcttccccca cagtttgtac
    86341 ttgattttct attaaagctg ttagaaaggc cccgcgcgct ttcttggaga tagttgagtg
    86401 aatcttcagc tgaccgcctc tatcctccat gaatttgaag gtcaccatct ttgctctgat
    86461 ttccttagaa aatacagtct caaactctga tgacaagaga gagaaaatca cttcttcctt
    86521 cttcagagct tcatcataga ctgccttcca atggctcttc aaagtcttac cagcgacttt
    86581 taatttcatc aaaaaatcca aacggtgagg agccatgggt gacaaggctg gaacaacacc
    86641 gtacaaagcc gaggtaatga agacatgatt ttcaagataa tcttgttccg cctcggtcag
    86701 cttatctctc ttaatgttgc ggtacataag cccatcaaaa agtttcaagg ctggatagtg
    86761 ttgagcagtt tgccttttca aagcttggat attttgaaat tcttccgccg ctttctcagc
    86821 cgataccttg tagaaactct ccaattgact ggcagaatag agagccaagg catcaagcac
    86881 ggtctgactt tctggtttta aaggaattgc ctcgatactt ggcaagtctg tgttcatttc
    86941 ttttgctgtt gggattaaaa ttttcatatt gatagtgtag catatttttt agccacaacc
    87001 aagaaattca tttgaaaaac ctcggtttga ccgaggtttt tctatttatt tgggccatcc
    87061 taaccagaca gccacgagaa caagggctgt gccagctaaa cttgttaaga tagataagaa
    87121 tttatctttt ttctccttga actgagaaaa acctatcttc aaagcgagca gtccgagtag
    87181 cattgaagca accatcaaat aaaaacccat ttctttgtcc tccattaggc ttaatttacc
    87241 ttattataac atatatttat taaataaacc ttttttatac tcaatgaaaa tcaaagagca
    87301 aactaggaaa ctagccgcag gctgctcaaa acactgtttt gaggttgcag atagagctag
    87361 agattttcga agagtattat tgtactttaa aggtcttgag ataattgtct tttcctactt
    87421 gaccttcgaa ggttttacca ttttcaaggt aaggaaggtc atcggatact gaagccttga
    87481 ccttgtatat cttgccatca actttaaaga agtagacagt gtctcccttg ataacagctg
    87541 atttgaggtc tgctactact cccttgatgc tttctgtcgt tgcattgtca atttcaaggt
    87601 cgtttttatt ggcatacttg ctgagcatct cttccactgt agtagcaacg ataacatttt
    87661 ggtactcgac tgcgtctacc agggcgtact ctttgaccaa gccagcattg tcctttaagc
    87721 ccatgatgta gagaggcttg tcattgaggt tgatgaggat tgggaaggtt gctttgtagg
    87781 atttctcctg aacagcacct tctgctgatt cacgggctga ttcttctgtc gcagaagcca
    87841 agctatactt agtgatttct cctgttcgca tattttcaag gatgaaacca agattactct
    87901 catccgcatt agccgacgtc acacctgtgt agagatagat gtcattaccg atagacaagt
    87961 aattatagcc cttggtagtc tgggtcacat ttttcttgga aatcatggca ttcaagaaac
    88021 cgtccttgta cttgccgttg tagttgattt gctcaatggt ttcctctgct ggatagaccc
    88081 tgtccaccca ttctggaaca tctgataagc tgtattcctt ggtttctcca tttgtagcat
    88141 ccaagataat gactgaagca ggacgaggaa ccgcaagtcc aaattgcttt tggtaaaccg
    88201 ttgctacata gaaaggattg ccctcatcgt ccacctcaaa agatggagtt ttaaagattt
    88261 tagtcgggta cttcaagcgc aggtgacgtt tgacatcacg gttaaaatac tccgagtctg
    88321 aatacttgat tggtgtcttc aagtccacca aatccgcatt tccagttacc atgtccacct
    88381 tgatatactc accgattccc ttggcttgat tgttaaacca tttgataggg tctgcatatt
    88441 ctagtggtgt gacccgataa ggtttcccat caattgtcaa ttgggtatag gtatctgccg
    88501 ctacgtattg cgacacctta tcggttaggg aacccaagta gcggtctcca attttttcag
    88561 cagtacttct atctaggata ggaaccttac tggtgtcact cctaggaaat tcagtaaagt
    88621 ctttttccgt aaccgtgact acattggcat aatttttagc ctgaaacatg ctggaagtta
    88681 ccaaggaaac caaagctgcc aagagaagaa ttcctccaat cgaagctaac agaattttcc
    88741 ctaaccgatt gattttgaaa ccctcaagat ttaaggcagc ttccgcctta ccgtggcgca
    88801 catgaaccgt tttgacaagg tttatcccct tgccaaagcc aaataagatt gccacaacta
    88861 acaaatgccc acagaggaag aagagaaatt cccagctggt caggttaagg ggcggtaaaa
    88921 agatatacca ggtcgttgcg ataaaaataa gttcaaagac tattcgtttc atttgctttc
    88981 ctcctaaatg attcgtcctt ccaagtgatc cagctcatgc tggcaaatct gagctgggaa
    89041 gcctgtcaag gtaatggtct gttcctgcca cttgctgtca cgataggcaa gccttatggt
    89101 ttcataacgc ttagttgatc tcacacctac caaggacaaa cagccttctt ctgcctcata
    89161 agatccttca aaggacagaa gcactgggtt aaacatgacc acgggaacca agccaagatt
    89221 aaagataatc acgcgcttct gcaccccaat catattggca gctagaccaa cacaggtatc
    89281 acgatttgct aagagtgtat cctgcaaatc tctggcaaga taaaggtcct cctgacttgc
    89341 cggctgagac acttgagata aaaataagat atccttcaca attttctttt ccacttcctc
    89401 acactcctct tgctttttac tatattatag caattatagc acaaaagccg ataactgcaa
    89461 accctttccc tcaactgtag caaaaagcca tccgaagatg acttttttgc tatttaattt
    89521 ctgtataagt tacttccaat ccacgcttaa cagctggacg attggcaatt ttttctgccc
    89581 attttactag attttgataa cttgaggcat ccaaaaattt tgcagaacct tggtaaagat
    89641 ttccttgaac taactgtcca taccaagacc agatagcaat atctgcaatc gtatagtcat
    89701 tgcctgcaat ataaggtttc tgagccaatt ccttatccaa taaatccaac tggcgtttca
    89761 cttccatcgt aaaacggtta ataggatatt ccaatttttc aggagcataa ttgaagaaat
    89821 gtccaaatcc cccacctaga aaaggtgctg cacctgcttg ccagaatagc caattcaaaa
    89881 cttctacctt ttccacagga ttacttggta aaaaggctcc aaatttctca gcaaggtaaa
    89941 gaagaatatg agcagactca aagactctta cgttttcagt acctgactgg tccaataagg
    90001 ctggaatctt ggaatttgga ttgagcttca caaagtctga gccgaattga tccccatcca
    90061 tgatagcaat cttatacaag tcgtaagccg cttccttaaa accagcttct agtaattctt
    90121 ccaataagat agtaaccttc acaccatttg gtgttcccag tgaataaagc tgaaaagctt
    90181 gttctccttt tggcaagttt tgttcgaaac gggcacctgc tgttggtctg tttaaccccg
    90241 taaaagctcc ttgattacta gcttcatcct gccatacggt cggtaattga tatgctgaca
    90301 tccgaaacct cccttaaatc gcattcttgt caaaaccgag tttgcgttga ataaacttaa
    90361 cgatttcgac gatgataatc attgagaagc ttccagccat aacaattccc cattgtgaca
    90421 agtctagttt ggttacgtgg aagattcctt caagcggttc tacaacgatt gttgccatga
    90481 gaaggataaa ggataccaag atggaccagt taaaggtctt agacttgaat gggccaactg
    90541 tcaagatgga ttggtagaca gacttgacat tgtaggcatg gaagagctga atcaaaccaa
    90601 gggttgcaaa ggccatcgtt agggcatctg catgaatagc atgattgtca cccacatgaa
    90661 ctgggtaagc aatcgcaagg ccataaacac tcataacaag agctgcttgg agtacacctt
    90721 gataaatgat agaactcaaa acaccacctg agaagaagct tgccttgcgt ccacgtggtt
    90781 tatgattcat gacaccaggt tccgcaggtt caacacctag tgcaattgct gggaaggtat
    90841 ccgttaccaa gttaatccac aaaagatgaa ctggctgcaa gacgtcccaa ccaaacaagg
    90901 ttgataggaa gatggttaat acttcagcag tattggcaga aagtaggtac tgaatagtct
    90961 tttgaatatt tgagaagacc ttacgtcctt cttccactgc gacgataata gtcgcaaagt
    91021 tatcatctgc aagaatcata tcagaagccc ccttagaaac ctctgtacca gtgattccca
    91081 taccgatacc gatgtctgct gttttcagag ctggggcatc attgacaccg tcacctgtca
    91141 tggcaacgac cttaccttgt ttttgccaag ccttgacgat acgaaccttg tgttctggag
    91201 acacacgggc ataaacagag tattgaccaa cgactttttc aaattcttca tctgacagtt
    91261 cattgagttc agcaccagtt aaaacgtgac cttctgtatc gtttgcgtca atgattccca
    91321 aacgtttggc aatggcttcc gctgtgtctt ggtggtcacc tgtaatcata attggacgga
    91381 ttcccgcttc cttagccaca cgaacagcct cagcggcttc aggacgttca gggtcaatca
    91441 tcccaatcaa accagtaaaa attaaatcat tttcaagctc ttcagaagtg agattttctg
    91501 gaatactatc gataatctta taagcacctg caaggacacg caaggcttga tgagccattt
    91561 cagaattgtt tgtatgaatg agatttgtaa ccttctcatc aatcggagca atatccccag
    91621 ccttatcacg aagaagacaa cgttttaaga gttggtctgg cgcacccttg actgctacaa
    91681 ggaaacgacc atctggcaat gggtgaactg ttgacatgag cttacggtca gagtcaaatg
    91741 gcaattcagc tacacgagga tatttctcta agaaaccttt gacatcatag cccttgtcca
    91801 aggcatattg gataaaggct gtttcggttg ggtcaccaat caagttacct tccacatcga
    91861 ttttcgtatc attggccaag acaactgaac gaagtagtgg catttcaaga cctagttcaa
    91921 tatcatcagc tgagtcatgt agaaccgcat cgtagaagac tttttcgact gtcatcttgt
    91981 tcatagtcag cgtaccagtc ttatcagaag cgatgatttc agttgaacca agtgtttcaa
    92041 ctgctggcaa cttacgaacg atggaatgtc gtttggccaa aacttgagta ccaagagaaa
    92101 gaacgatggt aacgatagca ggaagtcctt ctggaatggc tgcaacggca agggcaacag
    92161 aagtcaacaa ctcaccaagt ggatttttcc cttgaatgaa gacacccact acaaaagtaa
    92221 caagggcaat gaccaagata gcataggtca agaccttaga aaggttgttc aagttttgtt
    92281 tgagtggtgt atcagtctca tccgcatctt gaagcatacc agcaatatga ccaacttcag
    92341 tatacatacc tgtattgaca acaacaccca tcccacgacc ataggttacg tttgagtttt
    92401 ggaaggccat gttgacacgg tcaccaatac cagcatctgt cgcaagctcg actgacaagt
    92461 ctttttcgac tggtacagat tcacctgtca aggctgcttc ttcaatttta agagagttgg
    92521 cttctatcaa acgtaggtcc gctggtacca cgtcacctgc ttcaagggca acgatatcgc
    92581 ctggtaccaa ttctttagag tcaatctctg ccatgtgtcc atcacgaaga acgcgggcaa
    92641 ctggactaga catggatttg agggcttcaa tagcttcttc agcttttcct tcttggtaaa
    92701 caccaaaggc agcgttgatg ataaccacag ctaggatgat aatggcatct gcgatatctt
    92761 ccccaccaga agtcacgact gacaagattg ctgccgcaac taggatgata atcatcaaat
    92821 ccttaaattg ctcgatgaat ttgaccaaga ttgatcgttt ctcgccttct tcgagttcat
    92881 tgtgcccaaa ttcggcaagg cgcttttccg cctcacttga tgacaaacct tgctcggtcg
    92941 catccacagc ctgcaagacc tcttcagggc tctgagtata aaacgcttgg cgtttttgtt
    93001 cttttgacat gtgtctcctc cttgacattg tgtgcaaaac agactctctt tctgtcatat
    93061 cttttcacga caaacaaaaa gagacctgtt aatcataaca agtctcgctg tttaagatag
    93121 ggccggaaag catacttttc agcataaaat tcggaatgac gacactatca caggtttctg
    93181 ccagctactc ccttgagtag taccattata ccaaattttg gggagttttc aaagaataaa
    93241 aactgcctta tttgaatttt tccttgaaaa ccagtataat ggtagaatgc tatgtgacta
    93301 gaaaggaagt tgaatgaagc aatctatctc aaatctcaag ttagccgagc gtggagccat
    93361 tatcagtatt tcgacctatt tgatcttgtc tgcagccaaa ttagcaactg gtcatctcct
    93421 tcattcatcc agtttggtgg ccgatggttt taataacgta tcggacatca ttggaaatgt
    93481 ggccctctta atcgggattc ggatggcgcg ccagcctgca gaccgtgacc accgttttgg
    93541 tcattggaag attgaagatt tggcaagctt gatcacttct atcatcatgt tctatgtcgg
    93601 tttcgatgtt ctaagagata ccattcaaaa gattctcagt cgggaagaaa cggtcattga
    93661 tcctcttggt gcaactctag gaatcatttc tgcagcgatt atgtttgtgg tctatctcta
    93721 caatactcgc ctcagtaaga aatccaactc caaggcgctg aagacagctg ctaaggacaa
    93781 tctttctgac gctgttacct cacttggaac cgccattgcc atcctagcta gtagttttaa
    93841 ttatccgatt gtggataaac tggttgctat tatcatcact ttctttatct tgaagactgc
    93901 ctatgatatc ttcatcgagt cttcctttag tctttcagat ggctttgacg accgcctgct
    93961 cgaggactac caaaaggcta tcatggaaat tcccaaaatc agcaaggtca aatcgcaaag
    94021 aggtcgcacc tacggtagca acatctacct ggatattaca ctagagatga atcctgactt
    94081 gtctgttttt gaaagccatg aaatcgcgga tcaggtcgag tctatgctgg aggagcgttt
    94141 tggcgtcttt gataccgatg tccatatcga accagcacct atccctgagg atgaaatttt
    94201 agacaatgtc tataaaaaat tgcttatgcg tgaacaattg attgaccaag gaaaccaact
    94261 agaagaactc ttgactgatg attttgtcta tattcgccaa gatggagagc agatggataa
    94321 agaggcttat aagaccaaaa aagagttaaa ttctgctatc aaggacattc aaattacttc
    94381 catcagtcaa aaaaccaaac tcatctgcta tgagttagat ggtatcatcc ataccagtat
    94441 ctggcgtcgc cacgaaacct ggcaaaatat ctttcatcaa gaaaccaaaa aagaatagag
    94501 aaatcctttc atgagacggg atttttctat tcttttatac tcaataaaaa tcaaagtgca
    94561 aattaggaag ccggtcacag gctgtacttg agtcggcaat gtgaagccga catagtttgc
    94621 actttgattt tcgaatagtc ttaactatca aattcactga gatactcata gcgttcgtat
    94681 ttttcaagga gtgcttcatt tttctcatcc aattcttttt ggagagtagc cagcttacca
    94741 aagtcagagc cgttagcctg catttcctct tcaatagcag cgatacgttt ttccaaggtt
    94801 tcaatatcac cttcaatact tgcccactcc tgcttttctt ggtaggtcat gcgtttcttg
    94861 tcttctcgaa ccttgaccac tttttccttt tcggcctttt gcacttgatt ggccatatct
    94921 gtttcaaaag ctttttcatc aagatagtcg gtgtaatgac caaagaaagg acgaatcttg
    94981 ccatcctcaa aagcgagaat cttggtcgct accttatcca agaaatagcg gtcgtgactg
    95041 actgttaaaa cgggacctgc aaaaccttgc aagaaattct ctaagactgt caaagttgca
    95101 atatctaggt cattggttgg ctcgtctaaa agaagaacat ttggtttttc caaaagcagt
    95161 ttgaggagat aaagacgttt tttctcaccc cctgacaatt tctcaatcaa ggttccatgc
    95221 gtcgaacgtg ggaagaggaa ttgctccagc aactcagcga tggaagtcgt agaaccacca
    95281 ctggtcttga cctcctctgc cacttcctgc aggtaattga taactctctt gctttcatcc
    95341 aaaccctcaa tttgctgaga gaaataggcg atgcgaacag tttccccaat cacaacttgt
    95401 cctttagttg gctcaagact tcctgcaatc aagttgagca gggttgattt tccaacacca
    95461 ttgtccccaa cgattccgat acggtctttg gcctgcacca agagattaaa atcttgcaaa
    95521 ataggcttgt tttcataggc aaaggaaaca gcctgaaact cgatgacttt cttcccaatc
    95581 cgactggttt caaagttcat ggtcaagtct gtctcagcac tactgcctga aacttccttt
    95641 ttcagatcat ggaatcgatt gatacgggcc tgctgcttgg tcgcacgcgc ctgcggttgt
    95701 ctgcgcatcc aggccaattc ttgtttgtag agttgttctt ttttgtgaag aagagccgcg
    95761 tcgcgctcat cctgttccgc ctttaggcga acatagtcct ggtaattgcc ctggtactcg
    95821 gtcaagcctg cacgatccaa ctcgaaaatc cgtgttgaca aagcgtctaa gaaataacga
    95881 tcgtgagtga taaaaaggac ggtcttctta gaatttttca aaaagagggt cagccactca
    95941 ataatcgcaa tatccagatg gttggtcggc tcatccaaaa gcaagaggtc gtggttgcca
    96001 agtaagactt gtgccaactg tacccgtctt ctcagaccac ctgacaattc cccaacagga
    96061 gtagataagt cttgaatgcc caatttgcta agaacggtct tgacctgact ttcgatttcc
    96121 caagcttgga gagagtccat ctctgccatg acacgttcca aacgcgcctg cttgtcctca
    96181 ctatagtcga gcataatcaa ttcatactca cgaatgagct ggatttcctt gagttcacta
    96241 gatagaaccg tatccaaaac tgtctttcta tcatcaaaat caggatcctg agtcaagtaa
    96301 ccaatctggt aatcattttt agctgaaaaa ggactgacat ccccatcaaa tccagaaaca
    96361 ccagaaagga cgtccaaaag ggtggtcttg ccagtcccat tgacaccgat taaaccaatt
    96421 ctgtctaagt catggataat aaaggaaata tccctaaaaa cggtcttgtc accaacggat
    96481 ttacttagtt tttcaacgat aaaatcactc attttttctc cctcaggtaa gcatggatgg
    96541 cttcacgatt attctccaat tctccatcga caatggcaaa ctcaatctct gttaaaatct
    96601 ctcccaagtc tgggcctggc tgatagccat attccttgat caaaataccg ccattaatct
    96661 gaatctcttt cttgtcatgg atagtcaagc tttggtattt ttctgtgatg gcttgtgggt
    96721 tgacttcttt tccttgagct tgacgaagat tttcagcctg taaaagcaaa tctatgtcaa
    96781 agcgataaca atctcgcttg ctcaattctc cattttcacg cagagccaaa ataatcagca
    96841 aatcctgaac ttgcttggca aactggcgtg aggtcttcca agatttcaaa aatgactgcg
    96901 cattttcaat ctccaaagcc catagtaaag ccgcccaggc ttgttcagag gattcaaaag
    96961 taaaatcagt ctccaaatca aacagtctgt tgagcttgtc ctggctagat gccatatcag
    97021 ggagatagtc ataagcttga ctctcaatca tggaagccaa gccccttctc caaaatggag
    97081 ccagcaagag tttatcaaac tcgacgaagg tacgctctac agaaatgttc tccaaaagcg
    97141 gcgtcaaggt cttcatagct ttaaatgttt ctggttcaag tgcaaaacca agactagcct
    97201 gaaaacggaa accacgcata atccgtaaag catcttcgtt gaaacgctca ctagccactc
    97261 caactgctcg caagacttgc ttttccaaat cttctaaacc atggaacaag tcaacgattt
    97321 ctcctgtctc atccaaggca aaggcgttga ctgtgaaatc acggcgtttg aggtcttctt
    97381 ctagcgagcg cacaaaggaa accgcactgg gtctgcgata gtccacatag acatcctctg
    97441 tccgaaaggt tgttacctca tactcctcat ccccatctaa gaccaagacg gttccatgct
    97501 cgattccgat atcggctgtt cgcggaaaaa tctgcttggt ctcttctgga taagaagacg
    97561 tcgcaatatc cacatcgtgg atagggttat ggagaagggc atctcgaaca gagcccccaa
    97621 caaaataagc ctcaaagcct gcttctttaa ttttttctaa tactggtaaa gccttctgaa
    97681 attcagaagg catttgcgtt aatctcataa taagtgttct aatccataga caagctcatg
    97741 acgcttgaca acttctttaa ttcccaaatt gactcctgtc atgaaggaga tgcgatcata
    97801 ggagtcatga cggagggtca acccttctcc ctgattgcca aagatgactt cctgatgggc
    97861 taccaaacct ggcaaacgaa ctgagtggat gcgcatacca tcaaagtcag caccacgagc
    97921 accagcaatc agctcttcct catctgctgc gccttgctga atggactctc gaacctctgc
    97981 catcaactca gctgttttaa tggctgttcc actcggagca tcctttttct tgtcatgatg
    98041 gagctcaata atctccacat ttgggaaata tttggcagcc tgcgtcgcaa attgcatgag
    98101 taagacagca cccaaggcaa agttaggggc aatcaggcca cccaagtctt gggcacgaga
    98161 aaattctttt agctctgcaa tttcttcact cgtgaaacca gtcgttccaa ctactggagc
    98221 aaagccattt tcaagagcaa aacgtgtatt ttcgtaggca acagctggag tagtaaaatc
    98281 tacccagaca tccgcttcaa aaccagctaa atcagcctta tccttgaaaa caggaatatc
    98341 ctgccattct gactcagact caaaaggatc caaaactgcc accaagtcca agtctggatc
    98401 agtcaatacc atctgacaag cagcctggcc catctttccc ttaaaaccgg caataattac
    98461 tcgaatactc atctctactc ctgtctaaga tacaaagtcc gtaagaacac aaagtgaaaa
    98521 taggaattcc aatcaagaag tgtctacttc ttggaagaac tatctttttc acacagggtt
    98581 ccaggcgtgt tcaattatca agatacaaag gaccttagct gcctctgaaa aatagggaat
    98641 ggcactgact ttccacgaaa ggcaagacag gcatcttttt tcaagaggca ggtagtccgt
    98701 gttcaatttc taagatacaa ggcatcttaa ctagcctaga agcgccaact aaatcactgg
    98761 aatataaccc agagcaatac ttcctgctcc taggtgcgtt ccaatgacac taccaaatgt
    98821 agcaagtgaa acatccgaac ccaagccaaa atcaagcaag tgctgacgca attcttcagc
    98881 cttttcagga gcattcccat gaatgacaat gacccggtat tgacctgaag ccgttgtttc
    98941 cttgataatt tcaattaagc gcttggtggc cttcttttca gtacgaactt tttcgtaaac
    99001 ttcaatcaca ccttgatcgt taaaataaag gattggctta atgctaagca aattgcccaa
    99061 aatggcagcc ccatttgaaa ggcgtccacc ttttaccaaa tgatccaagt catctaccat
    99121 gataaaggct gacgtacggc tgatttgaat ggctagctta tcctgaatgc tggcaaaatc
    99181 atcgccctga tcacgccaat taaagacgct ttcaaccatg atgcctaggg gagcacttgt
    99241 aatcaaagtg tctgggaaag caatggttaa gccctcatag tcatcgacca tatactggat
    99301 attttggtaa aaacctgaaa ttccagaaga taggaaaagc cccaaggcat gtgtatagcc
    99361 ttgttctttg agcgaagtta agatctcatc taacttggca atacttggtt gactggtctt
    99421 aggcaattca gaagcctgag ccattttttg gtaaaattcc tcagcagaca gattgatgcc
    99481 ttcgacatat tcctcaccat caatattgac aggaatatcc aagacaaaca agtcttctct
    99541 ttgcaaggtc tcttcactga gataagcaga ggaatctgtg aaaacagcta atttcatatt
    99601 agaactccaa attaattcct ggtaagtcta atgcaatttc agtcacttcg taagtcaaac
    99661 gattgagcat gttcaaacat ggacgagcca aggtttccac ctcttcttgg ttcaattcac
    99721 ttggttcatt gacaatacgg ccatcgatat ggtttacttg tgagattgtt ccactaatga
    99781 caaacttatc aaatacaatc ataaagctca agatgacaat caaggaagtc acttgatttt
    99841 cttggtcatg ttggagcaat tggaaattca catccacctt ggtttcagga gctccatttt
    99901 cattttccca ttcaaaatta cgcgcatcaa aatgatactg actaacaaat tcttgttcac
    99961 gtttaagatt catgtctttc tccatcggct acaatattat aagctattgt accataattt
   100021 tttattttca tctagttttc taggatttag tcaatcccaa tttcagcacg aactacatct
   100081 gtgatggtat caacatagta gtctacttct tctgttgtag gcgcttctgc cataacacgc
   100141 aagaggggtt ctgttccact tggacgaaca aggatacggc cgttccccgc catttcttct
   100201 tccatcttct cgatgatggc cttgatagct ggcacttcca tggccttttc cttcatgacg
   100261 ttttccactc ggatattaac taatttttgt ggataaatcg ttacttctgc cgccaactct
   100321 gataagctct taccagtttc cttcatgatt ttagtcaatt gaactgctga taattgacca
   100381 tcacctgtgg tattgtaatc catcaagata acgtgaccag actgttcacc accaaggttg
   100441 taacctgatt ttctcatttc ttcaacaacg tagcggtcac caactgcagt aactgccttg
   100501 ttaatacctt cgcgattcaa ggccttgtgg aaaccaaggt tagacataac agttgtcaca
   100561 attgtatttt gagccaattg tcctttttca gaaaggtatt ttccgatgat gtacataatc
   100621 ttgtcaccat cgacgatgtc accattctca tcaacagcaa tcaagcggtc actgtctcca
   100681 tcaaaggcca aaccaatagc tgacccactt tctttgacca cttcttgaag ggcttctgga
   100741 tgtgttgaac caacattaag gttgatgtta agaccgtctg gtgtttcccc gataaccgtc
   100801 aattgggcac caaggtctgc aaagatttga cgggcactgg tagaagctgc tccattagct
   100861 gtatccaagg caaccttcat tccatcaaga ggagttccag ttgaaacaag gtatccttca
   100921 tacttacgca agccttctgg ataatctacc aaaattccta agccttctgc acttggacga
   100981 ggaagagtgt cttcctcagc atctagcaag gcttcaattt ctgcttcttt ttcatcatct
   101041 agtttgaagc catcaccgcc aaagaacttg attccgttat caagggctgg gttgtggcta
   101101 gcagaaatca tgacaccggc acttgctcct tcagtttcaa ccaagtaagc tactgctggt
   101161 gttgcaagga caccaagttt gtatacgtga atccctactg aaaggagacc tgccaccaag
   101221 gccgattcca gcatttcccc tgaaatacgt gtgtcacgtc ctacaaagac tttcggcgct
   101281 tccgtttcat gttgactaag aacatagcct ccaaaacgtc ctagtttaaa ggctaattct
   101341 ggtgttagct ctaggttagc ttctccacgg actccatcag tcccaaaata tttacccatt
   101401 gttataaaat ccttttctat ttttaattcg tttttgaact agttgctttc gttgacgaag
   101461 atgtctccga tgaactgctt gtacttgaat ttgatgtgct tgaacttggt gctactggtt
   101521 ttgtagtcac cttcattatt gtatcaaacg gagtgataac tgccggtaag acaacaccat
   101581 tgcgatcgat tgcctgcaaa ggtactgaac cactgtaatt acctgttatg cgttcgctag
   101641 ttggcaagac agcgataatc ttatcaattc tatccaatgt ctcttggtca ctcgtaatcg
   101701 acacttcttt atctgacacc atgacatttt caatttgtac ccgactatca atttgactag
   101761 ggtcaatctc tggtacaatc tttaccttat ccttctgagc cttcttacca atcttgactg
   101821 taattttttg cggagtcgcc acagcggtca gcccattggg taaatcttca atgctcaaag
   101881 gaacttcaat cgttccaaca ccggcatctg ttaggtcagc agtaaccttg aatttacgtg
   101941 tactttcttg catttcacta gctagcgata ggcgatttgc accagtcaag accactgata
   102001 cttctgaagc aaaaccgcta ataaaatact tatcactatt atagcgtatg tcaataggga
   102061 catttgttac tgtattagta taggtttccg tttttacctg cctagcactg gtactgtttt
   102121 gaaaattcgt cgccgtagca tagacaaata agacacaagc aaaaaagagt gaggatatga
   102181 tatataaact attttttttc atgtttccat cctcctagca atcgttcttt aaaactaaga
   102241 cccacttcct cttttggaag taagatttca cgtaattctg tttcaaattc atcaagtgtt
   102301 aggttgtgct taaaccttcc attataggtt atcgaaattc ctcccgtttc ctctgatacg
   102361 acaaaagtca aggcatctga gacttctgat aaaccgatag ccgcccggtg tctggtccca
   102421 aattccttgg aaatccctgt gttttttgtc aagggcagat aggcagacgt cacagcgata
   102481 cgttcttctt tgataatcac cgcaccatca tgtaggggag tgttgggaat aaaaatgtta
   102541 atgagaagtt ctgcagaaat cttagcatcc aagggaattc ctgtcgaaat atactcctgc
   102601 aaggtacgta cacgctgaat agcaaccaag gccccgattt tacgaggact catgtattca
   102661 acagacttaa caaaggcacg aatcatctgt tcctcagcac taataggggc attggaaaag
   102721 aaatctgtcg ctcttcccaa acgttccaaa ccagtccgaa tctctggaga gaagataaca
   102781 accgccgcaa taaccccata agtaataatt tgattgatta accaagaaat cgtagtcaaa
   102841 ccaatcatat ttgcaaggat ttgagctaaa ataaacacca aaactccacg taccaaaatc
   102901 ataatcttgg ttcctgcaat agcttttgta aaatggtata aaatataagc aacaatcaaa
   102961 atatcaatca gattgatagc tatcgtccat ggacttgcaa acaaactggt ccaatattgc
   103021 agattggata attgttgaaa attcatccct gatatcctcc ctatcaaaac actttcgtcc
   103081 tattatacca ttttctggta tttttttccc tatcctagtc cattttacat tgaacaaaaa
   103141 tatgataaaa taaactgact agaaaaaaca aaggagaaac tatgtctcaa ctctatgata
   103201 ttaccattgt gggtggtggt cctgtcgggc tttttgcagc cttttatgcc cacctacgcc
   103261 aagccaaggt tcaaatcatc gactctcttc cccagctagg tggacaacct gctattctct
   103321 accctgaaaa ggaaatccta gacgtaccag gcttcccaaa cctgactgga gaagagttga
   103381 ctaaccgctt gattgaacag ctaaatggat ttgatacccc tattcatctc aatgaaacgg
   103441 ttcttgagat tgacaaacaa gaagaagaat ttgccatcac aacttctaaa ggaagtcacc
   103501 tgactaaaac agttatcatc gctatgggtg gcggtgcctt caaaccacgt ccgctggaac
   103561 ttgaaagtgt tgagggctat gaaaatatcc actaccacgt ttctaacatt cagcaatacg
   103621 ctggtaagaa agtgacgatt cttggtgggg gagactcggc tgtggattgg gctttggctt
   103681 ttgaaaaaat cgcaccaact acccttgttc accgcagaga taatttccgt gccttggaac
   103741 acagtgttca agccttgcaa gaatcatctg taaccatcaa gacaccattc gcccctagcc
   103801 aactccttgg aaatggaaaa acacttgata aacttgaaat cacaaaagtc aaatctgatg
   103861 aaactgaaac cattgaccta gaccacctct ttgtcaacta tggtttcaaa tcttctgtcg
   103921 gtaaccttaa aaactggggg ctcgacctca accgtcacaa gattatcgtc aacagcaaac
   103981 aggaatccag ccaagcaggt atctatgcta tcggtgactg ctgctactat gacggaaaaa
   104041 ttgatctgat tgcgacaggc ctcggagaag ctccaactgc tgtcaacaac gctatcaact
   104101 acattgaccc tgaacaaaaa gtacaaccaa aacactctac tagtttataa aaaagaacca
   104161 cgagtcacat aggattcgtg gttttataat tcatccgcta tcttattgat ttttctgagt
   104221 ctgtgattga caccactttt ggtcagaggg gtgctgagac tatctgctaa ctgctggata
   104281 gagtagtctg ggtgctgaat tctcagttgc gccacttcct gcaaatctac tggcaaattt
   104341 tctaagccca tgatatcttt gattttactg atattgttaa tggtcttcat gctggcagaa
   104401 actgtccgag cgatattagc tgtctcggca ttattagccc gattgaggtc gttacgggtt
   104461 tctcgcaaaa tcttaacccg ctcaaaatca tcacgtgcct gcatggctcc gattactatc
   104521 aagaagtcca taatgtcttc tgctcgctgg agataggtca cagccccctt cttgcgctca
   104581 agcaccttgg catccagtaa aaactgttgg agaagggagg caattccttg cgcgtggtcc
   104641 agataaacag aactgatttc caactggtac ttgcctgact cagggtcacg aatgctccca
   104701 tttgccaaga aagcgccaca gagataggca cgacctgctt cctcatccga taaaatcgcc
   104761 tcatcaatac ctgtttccag gccaaagaaa gagtctgcca agtgcaaatc acttaacaaa
   104821 tcctgcacct tttcatctgt aaaaacggta tagacgcgat tcttgcgaag attgctccgt
   104881 tggtggtgac gaatttcaga tttgatttca tagaaatgga gaaaggactc atagaggtga
   104941 cgggccagtt tggcattttc tgtcacaact gacaaagtca agcccgaagt cgagagaccg
   105001 atgctaccag acattttgat aatggcagat aattcatgcc agctcagatg gtgttggccc
   105061 aggatttctt cttttactgc tactgtgaaa ctcatttttt cacctgtata atgcgcatca
   105121 actcgtccac aatcaaatct ccatcgtgga aggcaccgcc attttccaga cgaaggaagt
   105181 tagatgaaat cacgcgcgaa acttgcttac aaagacctac aaaatcgtgt tccacttgca
   105241 ccaagtattc atcaaaacgg ttggaattca tgtattcctg aggcactttt tcaatattca
   105301 ccaagacagt gtcgataaaa gggcgaccaa ggtgacgatg caagacttcc acgtggtcgc
   105361 tatctgtaaa gtgttccgtc tccccacgtt gggtcatgat attgcagaca taggcaattt
   105421 ctgccttggt ttccaaaaga gcccgcccaa tttccttaat cacgatattg ggcaaaatag
   105481 aggtaaagag ggaacctggc cctaggacaa tcatgtcact ttcaaggatg gtctgcacta
   105541 ctcgacggct ggccagaggc gtatcatcgt ttagggcatt ggtcacatag acattgtcaa
   105601 ttatgcctcg atggtctaca atatgactct ctccagccac ttctgtccca tcctgaaaga
   105661 ctgcatgaag ggtcaaagga tggtcactgg aaggataaat tttccctgtt gtatggaaaa
   105721 atttgctcaa taactgcatg gcattatagg ttgaaccctg catttctgac aagccagcaa
   105781 tgatgagatt tcccaatgga tggccagcaa aggctccggc atcctcagag aaccgatact
   105841 gaaagacctt ctcataaaac ttaggcatat ccgacatggc cacaaggaca ttacgaagat
   105901 cacctggcgg tgtcaactgt tgcatatttt ttcggagttc acctgaagaa ccaccatcat
   105961 ctgccaccgt cacgatagct gcgatttcca catctttttc ccgcagactt tttagaatga
   106021 cgggaattcc agtccctcca ccaatcaccg ttatctttgg ttttctcatg aacggtttac
   106081 cgtttccttt ctgcggtctt tgtcgcgatg cccttcatta acagaccaat tcttggataa
   106141 gtcctgcacc aagcgtttag caaatgccac actacggtgt tgtccacccg tacatcccat
   106201 ggcaatggtc aaaacggact taccttcctt ttggtaactt ggcagaatcg gctcaatcaa
   106261 ggccaataaa tgttgataaa agtcttctga ctcaggatgg ttcatgacat aatcataaac
   106321 aggttcatcc acacccgttt ggtttctcag ttctggtaaa taatagggat ttggcaagaa
   106381 acggacatca aagaccaagt ccgcatcaat cgggattcca tacttaaatc cgaaagacat
   106441 gacttcgata cggaaagact gggcttgttc ttggtctgaa aactgctctg caagggtttt
   106501 gcgcagctca cgtggagtga gttcagtcgt atccaccaca ttttggctca tatttttcaa
   106561 aggtgccaag agttcacgtt ccaacttgat tccatctaaa atacgaccgt ctgctgctag
   106621 tgggtgactc cgtctggttt ccttgtaacg agcgaccaat tccttatcag ccgcatccaa
   106681 aaagaggatt ttgaaatcca aaccatcttg attttccaac tcatccaaaa cagcttgaat
   106741 ctctgaaaag aaagaacggc tacgcatatc cactaccaag gccaacttag gattgtcttc
   106801 cttaatttca accagctgca aaaacttagg caagagagct ggcggcatat tatcaatggt
   106861 gaaataacct agatcctcga aggactgaat ggctacagtt ttccctgcgc cactcatccc
   106921 tgtcacaatc accaagtgaa gttgtttctt tgtcatcttt ttctccttat atcaaaagaa
   106981 gtttggcaac accaaacttc aactagctta tccaatctct gcgatgactt caatttcgac
   107041 ttttacatca cgaggaagac gagctacctc cacagctgaa cgagctggga attcctcttt
   107101 gaaggccgtt tggtaaacct cattaaaagg aacaaagtcg ttcatatcgc tcaagaagca
   107161 agttgttttg acaacatggt caaagtctgt tcctgcttct gccaaaatag caccgatgtt
   107221 tttcaagact tgctctgtct gttcttggat attctctcct acaatttccc cagtttcagg
   107281 ggatagggga acttgaccgc tagcaaacaa aaggttgcca acgatttttc cttgaacata
   107341 gggtccgata gcctttgggg ccttatctgt atgaattgtt tttgccattt tcttttcctc
   107401 acaatttttc taagattgca tcccaagcct catccatccc tgccttgctg acagatgaaa
   107461 agaggatgaa atcgtcactt gggtcaaagt ttaatttctt tttgattgct gattcatgct
   107521 tgttccattt accacgagga atcttgtccg ccttggtcgc cacaatgatg actggaatct
   107581 cataatactt gagaaattcg tacatctgca catcatctgc tgacgggtca tgacgaaggt
   107641 caactagact gacaaccgca cggagatttt cccgagtcgt taagtactcc tcaatcatgc
   107701 acccccactt ttcacgttcc tttttagaaa cacgggcata gccataacca ggcacatcca
   107761 caaagcgcat cttgtcatca atgttaaaaa agttcaggag ctgggtttta ccaggttttc
   107821 ctgatgtacg ggcgagattc ttacggttca acatagtgtt gataaagctg gatttaccaa
   107881 catttgaacg ccctgctagg gcaatctctg gcagttcatc ctgcggataa tgggacttat
   107941 tagctgcact gagcaagatt tcagcattgt gtgtattaag ttccatagtc acctctaggc
   108001 tgtttctagg atcggtttat ccgttccatc gacagtttct ttagtgatgc gaaccaattt
   108061 cacattttcc tgactcggca cctcaaacat aacatctagc atggtttctt cgataatgga
   108121 gcgaagtcca cgcgcccctg tcttccgttc gattgcttta ttagcaatct cttgaagggc
   108181 ttcgtcgtca aattccaact caacatcatc ataagaaagc aaggtttggt attgtttcac
   108241 caaggcattt cttggctctt tcaagatgcg aaccaagtca tcaacagtca attgctcaag
   108301 agccgcaaaa acaggcaagc gtccaatcaa ctcagggata ataccaaatt tttgaatgtc
   108361 ttcagcgatg atttcttgca tgtatgagct gttttcgtca atcgccttat tgttttgacc
   108421 aaatccgatg actttttcac ccatacgttg tttgacaatt tcttcaatac catcaaaagc
   108481 accacccacg atgaagagga tattttttgt atccacttga atcatctctt gttgtggatg
   108541 tttgcgtcca ccttgaggcg gcacgctagc aacagttccc tcgataatct tgagaagggc
   108601 ttgttgcacc ccttcaccag aaacatcacg tgtgatagac acattctcac tcttcttggc
   108661 aatcttgtca atttcatcca catagataat gccacgctct gcacgttcga tgttaaagtc
   108721 agcaacctgc aagagtttga ggaggatatt ttccacatcc tcacccacat aaccagcctc
   108781 cgtcagagct gtcgcatccg caatagcaaa aggtacattc aagctcttag ccaaggtctg
   108841 ggcaaggaaa gttttccctg aaccagttgg gccaatcatc aaaatgtttg acttctgcaa
   108901 atccacatct tctgactctt cgcgtgtatc gtggaaattg atgcgtttgt agtggttata
   108961 aaccgccact gccaaggcac gcttggcacg atcttgacca attacatagt ggttcaagat
   109021 atggaggagt tcaattggtt ttggcacctc agacaagtct gccaagactt cctcaaccaa
   109081 ttcttctcga atgatttcct gagctaactc cacgcattca ttacaaataa aagcattgtt
   109141 gccagcaatt attttttgta cttcttcttg gtttttgcca caaaatgagc aataaaccat
   109201 catatcattt tttctatttg tagacatgat ttccttccat tctatactgt cattctatct
   109261 aaaataaggt catgtaaaaa gcatgaatac tattgaccag attggtaaag gcatttaacc
   109321 aaaggaggat agaaagcccg taacgctttt tacgaaaagc ttgtgctcct gccagaaagc
   109381 agatgaaaca cagaaaagcc gtgaatagac caaataaact ccgttccatt agacttcctt
   109441 tctcttgcgg tattggatgg taaaatcata aggattcttc tcatctttgg cgtaaaattt
   109501 gcttgaaact gtctcaaaaa gagacaagtc aagttcttca gggaaatagg tatctccttc
   109561 cacccgagca tgaatgtgag tgacaatcac ttcatcaagg taagcttcaa aagcctgaaa
   109621 aatttgcttc ccaccgataa tgtagagatt cttttcttga gcctgatacc agtcaagaac
   109681 agactggacg tcctgaaaag tagcaacccc atctatcttt tcttccggat tacgcgtcaa
   109741 aatcaaggtt tcccgttttg gaagcaagcg acgccccatc ccatcaaagg tcacacgccc
   109801 catcaagata gcatgattca gagttgtttc tttaaagtgc tgcaattctg ctggcaaatg
   109861 ccaaggcaga cgattttcct taccaatcac accctcttca tcctgggccc aaatagctac
   109921 gattttctta gtcatgcttc catccttttc actgatagta ctattttatc aaaaaactca
   109981 aaaaaagact ggtttggaat agcttacaaa atagaaaaaa tctgtaagaa atttcctaca
   110041 gatttatcta tgtttcctta tttcttacaa accaggtgct tgtccaagtt cggctgcaag
   110101 catccaaatt gttttatctg tttcagtttt agcgcctgca aagataccgt ttgttacatc
   110161 gtcaccttct tcatcagtga catccaaacc tttttggaaa agttctgaca agtaacggta
   110221 gataacaaga acacgttcca agctttcttc aacattacgg tattcaccag cttcttcttc
   110281 gatttcacta ttttgaagga actctgtcaa tgtagagaat gggcttccac cgagtgtaat
   110341 caagcgttcg ctgatttcat ccaattgacc gtcaagagct tccatgtact catccatttt
   110401 tggatgccat acaaggaaac cacgaccatg catataccag tgcacttggt gcaaagcaac
   110461 gtgagctaca tacaaatcag caacagcttg gttcaagact tcctttgttt ttgccaatgc
   110521 tactggcgct gcttttgtca atgatgttac gtcttttact gcttcttttt tcaattctac
   110581 cattttcttt acctcattca ttattttttg taaccacttc ttaatgatta ctaccttagt
   110641 atactactaa ggaaaaccaa tagcaagaca aagaacttat atgagaaaag ttgcaataga
   110701 ctgataattt aagaattctt tagaagacaa aaagagggtt ggatgcttat agtcaatgga
   110761 aatccatcag caaactagga aactaaaccc agcttgacca aagcacaagt tttgaggtcg
   110821 caggtagaac tgaccaagct gatgtggttt gaagaaattc cccaatacta taatagattg
   110881 aaataagatg aaaccaagtc gattaggaaa ggtaaatgaa tttctaaaat gttttaccaa
   110941 tcacaaggta tttgtctata ttcaatctac tatataagct tcatttccga gcaagatctt
   111001 gaatttactc gaggatgaac tatcataaaa caaagaaaaa gagggtgcta ctatcacatc
   111061 cctctttcaa atcatcgctt aataccaaac gctgacatct acgcgccctt ggattccttt
   111121 catgtattca gaagaggtat attgccaacc tttttttcct gaataatgag ggttttccca
   111181 ttctaaagca ttcgtatagg ccgctaccca gtttacatgt tttaaaatat ctgggtgttt
   111241 taaacgcgtc tgtaataaac tacgatagct atagacatac acattttgat aacccgcctg
   111301 cttcatcgtg tccatgtact tgttgatgat tttaacccaa gtgcctgtat cacttggagc
   111361 tctcttactc ttatttacat attcccaatt ctcaacatca taatagatag ggtaagacag
   111421 gttcatattg tatttcttta taagttcaat ggtctgttta gcgtcactct cagcatcggt
   111481 ctcattttca gcataggtat agagatagac accataagga attcccagac ggtttaactc
   111541 cttaatgtta tgcgccaatt ccttgtcttc tttaccgcta taacctagac gaacaatgac
   111601 accatccact tcgttctcat caataacctt tttccaatca ttgatacgac cattgtgctc
   111661 actaatatca atgactttct tagcatgttc ggttcccact tgttcttctc tattattaaa
   111721 gaaatagcgc ttaccatctc tgtgaaccca gccgacattc aaatctttct tttctttgag
   111781 ctcaccagag gccgcaaaga tatagcgagt accatccata atcacttcgt cagtcgccat
   111841 ggcaccactt ccagtcaaat agtagttgcc ttgccactca ttccgagcca tatagcccca
   111901 cttcttgaaa tagtaccatt tgccgcccac tttttgccac tcttggttag cataagttcc
   111961 atcggatttt agataaaaat aagcagaata ggctggatca tagagccatt cattttgcat
   112021 catagctcct tgaccattca agaaataact tccttgccat tggcttttag ccatgtaacc
   112081 ccacttcttg aagtagtacc acttatttcc aatcaattgc cattcttgat gagcataagc
   112141 cccatctgac ttgagataaa accaactctt gtaattactg tcatagatcc actcgttttg
   112201 ggccatatat ccacctgatt tcaggtagta atttccatgc cattcattct gcgagtaacg
   112261 accatctgaa ttaatataaa accaagcctt atagtagttg tcaaaaatcc attcactttg
   112321 agctttagaa ccatcatcct ttacataata atccccatcc cagtgggcag cagcattagt
   112381 ctgtacttct gcgctcgttt gcgttttatt agaagactga ttttgctctg aactacttga
   112441 agctgtcgtt gtatcctgcg aagtttttgc tacttcagtt tcatttgcag cgacatgact
   112501 agttgccaag cctagtaaac agatacttgc taatccaatt tttgttttca atctttgctc
   112561 tcctataaaa aatgtaacag acatctgaat gctgttccac ctagcttttg ctacttactg
   112621 attattttac aaagtcaagc aaagctaaga agctttcagc ttcaagtgac gcaccaccta
   112681 caagggcacc gtcaacgtct gggcaagcca tgtatgaagc aacattttca ggtttaacag
   112741 aaccaccgta ttgaacacga actttgtctg cgacttcttg accaaagtca gcagctacaa
   112801 cgtcacgaac aactttacac attttttgtg catcgtcttg tgaagctgat ttaccagtac
   112861 cgatagccca gattggctca taagcgataa ctgaggcagc aacttgttca gcagtcaatc
   112921 cagccaatgc agcagatact tgagcaccta cgaattcagc agctttacca gcttcgtaag
   112981 tttcaagtga ttcaccacaa cagatgattg gaagcatacc gttcgcaaag attgcttttg
   113041 cttttttgtt gatatcttcg tcagtttcat ggaagtagtc acggcgttct gagtgaccga
   113101 taacaacgta atcagtaccg atttctttca aaacttgtgg gctagtttca ccagtgaaag
   113161 cacctgcatt ttcaaagtag cagttttgag cagcaacttt aaggttcgag ccttttgcaa
   113221 cagcaagaac agttgtcaaa tcaagagctg gagcagcgat acctgcttca acaagatctg
   113281 atgaaggaag ttttgatgca actgcttcaa cgaatgcttt agcttcttct ggatttttgt
   113341 tcattttcca gttaccagcg ataaatggtt tacgtgacat ttcacatacc tcttttttca
   113401 atttattttc tatttattct atcacaatta tacacagctt gcaaatctta ctcggatttc
   113461 aagcctactt gcatggatta atccttccag agatccatgg catttctgaa atctgcagat
   113521 acctgtgtca actgaggatt gcttgcttct ctttctgctc tcttggcctc aatttgagcc
   113581 acactcttga ttccttcatg gcgccagttt ctcaaaattg cctgaatgta cttccagttt
   113641 ggttttccat tcaaaacagc ttcacgaaga gcctccttaa tcaagtcagc actggttcca
   113701 tcttccttta gtgtcttggt caaatcctca atctcaaaag gcgtcaacaa tcgtcccaac
   113761 tcctgctgga aggtttccac caaatccttg agctggtttt gaggtgttag ctggtctgaa
   113821 cttgaacgaa ctgctccaaa caggtcatcc aaacgttcca aggccaaact agcatcaaag
   113881 aacaattcaa tttcgccatt taattcgata gtacgatact ggagcagtcc cctttccgtc
   113941 agattagaaa tggactggtt gacatccgaa atttccttgc caatcctttc agcaatctgg
   114001 cttggcgaca tttcttctaa gcctgtcgta ttttgcaaat agaaaaattg ccaaaccaga
   114061 aaatcgtcgc tagaaggaaa gagttcctta aaatgcaaga gcagggcact cggtaaaacc
   114121 aaggtacctg atttaaaagc gtctaaatat gtcataattc ctcttataaa tacccatatg
   114181 cagatgcatc atcttctatc tttctccaat caaaaggcgt ctcctgatag accgcatggt
   114241 ttacccaatt actgaaaaag agggctgcag atgaagacca acaaagacaa ggtacctgat
   114301 taacatcatc atctttaaag taattttctg gaatatgtgg gtcgaaacct gcatcacgat
   114361 ctcgaaaata ctcttttgcc aaggtatcac ggtcatactc caaatgacca aaactataaa
   114421 tttctcgtaa atcacgactg gccaaaatag aaaccccaac ctgaggtcct tctgataaaa
   114481 tctcgagatt ggtcttgttt aagacctctt ccttagaaat ctccgtgtgc cgtgaatgag
   114541 gggatacata gctatcatca aagcctctaa atagaaggtg accctctttt agggtgtcct
   114601 gaggataaat acctgatagc ttactgtcca tctggtattt ttctacacca tagcgcagat
   114661 aaagcccagc ctgagccccc caacagatat gaagggtcga atagacatga gtcttagacc
   114721 actcaagcat ctgtctaaat tcctcccaat agtccacttc ctcaaatggt aaatgctcaa
   114781 ctggagcacc cgtgatgatc atcccatcaa aatactcatc cttgacttca ggaaaagttt
   114841 tatagaaggt ctccatgtgc tctgaacgag ttgttttaga acggtggctc tccatataga
   114901 gaaaatcaat atccagttgt aggggtgtat tagccaagtg gcgcaacaac tgggtctctg
   114961 tgaccatttt ctgtggcatg agatttaaaa ttaaaatctt caaaggacgg atatcttggt
   115021 gggcagcacg ttgatcatcc atgacaaaga tattctctgt ccgtaaaatc tcaacagctg
   115081 gcaatttttt atcaattcga atcggcataa ccctctccta actgtactct ttcaggaata
   115141 attgcatatg tttgaagaca aatctcaaac acttagtcct tttattatac tgtaagaaga
   115201 tatagttttc aattatagtt tttctctaac tagttatagt ctatttttat atcctagtgt
   115261 agagaaaaca gccctaggga ctgttttcat taataatgca taagaacttt gtagtcgtag
   115321 tcaccaattt tttcacggcc gttcaattca tccaattcaa caaggaaggc acaacctgcc
   115381 ataacaccac caagtttttc aatcatctcg atagttgcct taacagttcc acctgtcgcc
   115441 aaaaggtcat ctacaataag aacacgttga cctggcttaa tggcatccgc gtgcatagtc
   115501 aaggtatcga caccgtactc tttttcatag tcagcagaaa taacttcgcg tggcaattta
   115561 cctggcttac gaacaggcgc aaaaccaatt cccaactcaa aggcaactgg acaacccacg
   115621 ataaatccac gagcttcagg tcccacgatc atgtcgactt tcttgtcagt agcatactga
   115681 acgatttcac gaacagcgta gctataagca tttccatcag ccatcaaagg actaatatca
   115741 cggaaggtaa tgccttcctt tggataattt tcaattgttg caatgtaatc ttttaaattc
   115801 atctttttct ttctttcaaa gttttttact ctctattata gcatattttt taagaaagaa
   115861 aaaaggaaaa gttaacttca ataattatct aacgttttga cgatttataa ctagccatcg
   115921 caataaagcc caatttctgt ttattcttag caaacatttt atacatagtt aaaaactgct
   115981 ttctattctc ctttttacaa gcatttacac aaattttcaa agttcctagc aaaccttcgt
   116041 cataaatcat acccgataat ttcattaatg tcatttcacc agtcaatgct ttcacatcac
   116101 aataacctga ttctatcatc acctgttccc aaccatcttg agttaaagga cctacattta
   116161 catgaattgc ttgtgataat tcctgtctga tagactcttt agcttcctta agaagcacat
   116221 catgtgtcaa gagaagacct ccaggtttta atacccttag atattccatt acacattttt
   116281 tcttagcttg atcggcttgc atagtcagca tagcttcatt tataacaata tcaaaactag
   116341 catcttgata aggaagtttc attgcatttg ctctttcaaa actgattaaa tgagcaacac
   116401 ctgccgttcc agcagatttt ttagccactt ctaaagcttg agcatccata tcaatagcag
   116461 ttatcttgca accaaaacgc tgtgccaact caattgctgt agttccccta ttacacgcaa
   116521 cctctagtat tctcttttct tttgaaaatc ctccttctgc aattaaccaa tctgtggcac
   116581 gctttccacc tggacgtaag cgttttttcc ccaattttgc taaaaactta tgacctgctt
   116641 ctgacatttg aacacctctg ttttttcttc attataacac aaaatgatat tatctgacaa
   116701 ttctaatcta ttatttgata aactagaatc aaaagcatac aagtcaccat aactatagca
   116761 taaatttatt attacctctc tagttcgaat tcaattgctc acgtagttta cgcatatagc
   116821 ttcacttgta gatggtaccc acaaataata ctcaatggaa atcaaagaga aaactagaaa
   116881 actagccgta agctgctcaa agtacagctt tgaggttgca gataaaactg acgaagtcag
   116941 taatcatacg tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtatta
   117001 aagagcatca caatatagat gcataatatg acttccctag caaaaaggag agaagcattt
   117061 tgtttctctc cctagattaa ctgatatctc tccactacaa ttgataaaga gatatctttt
   117121 cttgtagtgc tttgaatttt atttatttta gtagattgtt ttttcagttt ctacatcgaa
   117181 gaagtgtgct ttgttcaagt caaatccaag ctcaactgtt gcacctgttt gcaagtagtc
   117241 acgagcatca acttttgcaa caaactcgtc tttaccaact tggcagtaaa ggtgagattc
   117301 tgaaccaagc agttctgata cagagatagt cgcttttaca acacagtctg ggaatgtttc
   117361 aaggaaagca ggttctgcat tcacgtcttc tggacggata ccaaagatca attcttttcc
   117421 ttcgtagcct ttttcacgaa gaactttcaa tgctccttct ggcactttca aacggaaacc
   117481 gtcagaaaca atttcgctac caaccaattt cacgttgatg aagttcatag ctgggcttcc
   117541 gatgaatcct gcaacgaatt tgttaactgg atttttgtaa acttcttgag gagtaccgat
   117601 ttgttctaca cgtccgatag tacctgtacc agcagggttc ttagtagctg acataataac
   117661 gatacggtct gcaagtgtca tcgcttctgt ttggtcgtga gttacataga tagttgtagc
   117721 tccgatacga cggtgaattt tagcgatttc agcacgcatt gatacacgaa gtttggcatc
   117781 caagtttgac aaaggttcgt ccatcaagaa tactttcgca tcacggacaa tcgcacgccc
   117841 catggcaaca cgttgacgtt gaccacctga aaggtcagct ggtttacgtt ccaagaattc
   117901 tttcaatcca agtatttcag ctgcttcttg aacacgtttg ttaatgtctt ctttgctgta
   117961 tttacgcaat ttcaaaccga aagccatgtt gtcataaaca gtcatgtgtg ggtaaagagc
   118021 gtagttttgg aataccatgg cgatatcacg gtcttttgga gctacgtcgt tgacaaccac
   118081 gccatcgata gatgcagtac cttctgtaat gtcttcaaga ccagcaatca tacggagtgt
   118141 agttgattta ccacatcctg aaggtcctac gaaaacgata aattctttat ctttgatgtt
   118201 caagttgaaa tcttcaactg aatagtgttc gctgtttgga tattttttgt aaatattttt
   118261 aagattcaat tctaccatga ggtgaactcc ttttgtcttt tgataattat attatagatg
   118321 aaaacgcttt atatttctat ggcaagatga ccaaaaaaat aaaaaagttc tgtgcaactt
   118381 gcacaaaact ttagagaata tcggagaatt tgaagaagat aatctttatt attcttctat
   118441 gacaaaagca gaaacaagga tcagttttcc catattttcg ctgattctcc actacatttg
   118501 actggattct aattttttag agaaatacaa aagagctagc tttagctaac tcttttccta
   118561 tgcggagaga gggacttgaa ccctcacgac ctaaagcggt cacaggatcc ttagtcctgc
   118621 gcgtctgcca attccgccat ccccgcatcg attactttac tagtatatca acttttggga
   118681 tgcttgtcaa cacttttttt caaatttttt cattttcacc aaccaggtta ctcagaaagt
   118741 tcatttagat tttcatctac taacttagct ccgagtgtat ttttgaaatg acctagggca
   118801 aattgatgat tttcaggcca gatggaagca acagctggtt taacaatctc gatgtcatat
   118861 cctagattat aggcatctat agctgtatgt aggacacaga tatccgtcaa gacacctgtt
   118921 aagataacgg tagacactct acgctctctc aaacgaatat ctaggtcagt ccctgaaaaa
   118981 gctgagtaat ggcgtttatc catccaaaag acacgactgt ctgaaccatg ctcttgataa
   119041 aagatcccca aatctccata taaattccgt ccactcgtcc caatcagatt atgaggagga
   119101 aataacttac tttccggatg gaaacaatcg ttttcttcat gagcatcaat agtaaagaag
   119161 atataatctc ctcgttcaaa agctaatcga gttaccttgc tgatggcatc cgaaatcgcc
   119221 tgagctggag cacctgctgt cagcttccca ctatcagcaa caaaatcttc tgtataatca
   119281 atcgaaatta aagcctttgt cattagtaat ctcttttctt cacttcttca aaaatatctg
   119341 aaatcaagac cttaagatag gttcccttca ttccaagtga gcgactttca ataatccccg
   119401 cagactcaag tttacgaaga gcattgacaa tcacagagcg agtgattccg atacgatctg
   119461 caatcactga cgcagtcaac ttcccttcat ttccatttaa ttcccctaaa attgctgaaa
   119521 cagcacggag ttcggagtaa gaaagggtat tgaccgccat ggtgacagca gtacgacgac
   119581 gaatattttt ctcatcttct tcacgttgga agttaagaag ctgaatccca acaacggtac
   119641 tggcaatctc aacaagaacc aagtcctcat cttcgaattt tttatcatta cgccaaataa
   119701 tcaaagaacc aaggcgaatc cccgatacat gaatcggtgc aatagtcgtc aagccatctg
   119761 gaaaatcatc tctactctca acagggaaaa tactcatatc atgctcaaca ggcaagtttg
   119821 cttctgtttc gtaaatcata ttagcccctt gaacgtagtc atctgggaaa atcttagttt
   119881 ggaagaattg ctctacgcga tctgtatttg ttttataacg cataaaatag ccaagcagac
   119941 gtcccttact attgataatg caggcattgc aatgaataat atccgctaac tgacgcgtaa
   120001 tagcgttgta agggagctca tcctgcaact gctcctctga gcgtttcaaa attgatgtaa
   120061 tttttctagt tttttctaat aaatgtgcca tttttcacct cgaatttaat cgctatcatt
   120121 ataacataaa aacgtctctt tttcaataat tatctgaaaa ttccttattg acttgcattg
   120181 acttaca
//
LOCUS       DAVKWS010000002        79176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKWS010000002 DAVKWS010000000
VERSION     DAVKWS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 79176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.002"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            84..740
                     /locus_tag="VW852_000130"
     CDS             84..740
                     /locus_tag="VW852_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV0271338.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            730..1053
                     /locus_tag="VW852_000131"
     CDS             730..1053
                     /locus_tag="VW852_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV0271339.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1157..1987
                     /locus_tag="VW852_000132"
     CDS             1157..1987
                     /locus_tag="VW852_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271340.1"
                     /translation="MKKIVKYSSLAALGLVAAGLLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2141..2995
                     /locus_tag="VW852_000133"
     CDS             2141..2995
                     /locus_tag="VW852_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0271341.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            3095..4468
                     /locus_tag="VW852_000134"
     CDS             3095..4468
                     /locus_tag="VW852_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV0271342.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4461..5522
                     /locus_tag="VW852_000135"
     CDS             4461..5522
                     /locus_tag="VW852_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV0271343.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVTANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5524..6216
                     /locus_tag="VW852_000136"
     CDS             5524..6216
                     /locus_tag="VW852_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271344.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6246..6815)
                     /locus_tag="VW852_000137"
     CDS             complement(6246..6815)
                     /locus_tag="VW852_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0271345.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6950..7564
                     /locus_tag="VW852_000138"
     CDS             6950..7564
                     /locus_tag="VW852_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0271346.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7566..9212
                     /locus_tag="VW852_000139"
     CDS             7566..9212
                     /locus_tag="VW852_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0271347.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9224..10510
                     /locus_tag="VW852_000140"
     CDS             9224..10510
                     /locus_tag="VW852_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0271348.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATAGHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVASVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10562..11095
                     /locus_tag="VW852_000141"
     CDS             10562..11095
                     /locus_tag="VW852_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271349.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11173..11643
                     /gene="nrdI"
                     /locus_tag="VW852_000142"
     CDS             11173..11643
                     /gene="nrdI"
                     /locus_tag="VW852_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV0271350.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11936..13198)
                     /locus_tag="VW852_000143"
     CDS             complement(11936..13198)
                     /locus_tag="VW852_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV0271351.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTTILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13508..13966)
                     /locus_tag="VW852_000144"
     CDS             complement(13508..13966)
                     /locus_tag="VW852_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV0271352.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(13963..14403)
                     /locus_tag="VW852_000145"
     CDS             complement(13963..14403)
                     /locus_tag="VW852_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0271353.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14759..16708
                     /gene="mutL"
                     /locus_tag="VW852_000146"
     CDS             14759..16708
                     /gene="mutL"
                     /locus_tag="VW852_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV0271354.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMALVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTFYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17033..17500)
                     /locus_tag="VW852_000147"
     CDS             complement(17033..17500)
                     /locus_tag="VW852_000147"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV0271355.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17501..18706)
                     /locus_tag="VW852_000148"
     CDS             complement(17501..18706)
                     /locus_tag="VW852_000148"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV0271356.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18726..19361)
                     /gene="ribE"
                     /locus_tag="VW852_000149"
     CDS             complement(18726..19361)
                     /gene="ribE"
                     /locus_tag="VW852_000149"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV0271357.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19346..20446)
                     /gene="ribD"
                     /locus_tag="VW852_000150"
     CDS             complement(19346..20446)
                     /gene="ribD"
                     /locus_tag="VW852_000150"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV0271358.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20580..20694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            20851..21444
                     /gene="ruvA"
                     /locus_tag="VW852_000151"
     CDS             20851..21444
                     /gene="ruvA"
                     /locus_tag="VW852_000151"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV0271359.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21454..22017
                     /locus_tag="VW852_000152"
     CDS             21454..22017
                     /locus_tag="VW852_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV0271360.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWRGLK"
     gene            22014..22691
                     /locus_tag="VW852_000153"
     CDS             22014..22691
                     /locus_tag="VW852_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0271361.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKGIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(22828..23496)
                     /locus_tag="VW852_000154"
                     /pseudo
     CDS             complement(22828..23496)
                     /locus_tag="VW852_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
     gene            complement(23513..24466)
                     /locus_tag="VW852_000155"
     CDS             complement(23513..24466)
                     /locus_tag="VW852_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV0271362.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            complement(24725..25105)
                     /locus_tag="VW852_000156"
                     /pseudo
     CDS             complement(24725..25105)
                     /locus_tag="VW852_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
     gene            complement(25360..26043)
                     /locus_tag="VW852_000157"
     CDS             complement(25360..26043)
                     /locus_tag="VW852_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV0271363.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPNMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(26055..26999)
                     /locus_tag="VW852_000158"
     CDS             complement(26055..26999)
                     /locus_tag="VW852_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0271364.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            27131..29962
                     /gene="uvrA"
                     /locus_tag="VW852_000159"
     CDS             27131..29962
                     /gene="uvrA"
                     /locus_tag="VW852_000159"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV0271365.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            29955..31016
                     /locus_tag="VW852_000160"
     CDS             29955..31016
                     /locus_tag="VW852_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0271366.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMDLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLVPKELIVI"
     gene            31148..31546
                     /gene="spx"
                     /locus_tag="VW852_000161"
     CDS             31148..31546
                     /gene="spx"
                     /locus_tag="VW852_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV0271367.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            31612..32181
                     /locus_tag="VW852_000162"
     CDS             31612..32181
                     /locus_tag="VW852_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0271368.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLIIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            32268..32534
                     /locus_tag="VW852_000163"
     CDS             32268..32534
                     /locus_tag="VW852_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV0271369.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            32538..32957
                     /gene="ruvX"
                     /locus_tag="VW852_000164"
     CDS             32538..32957
                     /gene="ruvX"
                     /locus_tag="VW852_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV0271370.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGKFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            32973..33278
                     /locus_tag="VW852_000165"
     CDS             32973..33278
                     /locus_tag="VW852_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV0271371.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            33691..34941
                     /locus_tag="VW852_000166"
     CDS             33691..34941
                     /locus_tag="VW852_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0271372.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTCLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            34938..35456
                     /locus_tag="VW852_000167"
     CDS             34938..35456
                     /locus_tag="VW852_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV0271373.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDG
                     DRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDM
                     DRDPSDQIVLTK"
     gene            35602..37134
                     /gene="cls"
                     /locus_tag="VW852_000168"
     CDS             35602..37134
                     /gene="cls"
                     /locus_tag="VW852_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV0271374.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGINLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            37392..38930
                     /locus_tag="VW852_000169"
     CDS             37392..38930
                     /locus_tag="VW852_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV0271375.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            39050..41257
                     /gene="nrdD"
                     /locus_tag="VW852_000170"
     CDS             39050..41257
                     /gene="nrdD"
                     /locus_tag="VW852_000170"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV0271376.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALRKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKGENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            41269..41409
                     /locus_tag="VW852_000171"
     CDS             41269..41409
                     /locus_tag="VW852_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271377.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            41453..41959
                     /locus_tag="VW852_000172"
     CDS             41453..41959
                     /locus_tag="VW852_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271378.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            41952..42542
                     /gene="nrdG"
                     /locus_tag="VW852_000173"
     CDS             41952..42542
                     /gene="nrdG"
                     /locus_tag="VW852_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV0271379.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            42539..43156
                     /locus_tag="VW852_000174"
     CDS             42539..43156
                     /locus_tag="VW852_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV0271380.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            43423..43731
                     /gene="rpsJ"
                     /locus_tag="VW852_000175"
     CDS             43423..43731
                     /gene="rpsJ"
                     /locus_tag="VW852_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV0271381.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            43948..44574
                     /gene="rplC"
                     /locus_tag="VW852_000176"
     CDS             43948..44574
                     /gene="rplC"
                     /locus_tag="VW852_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV0271382.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            44599..45222
                     /gene="rplD"
                     /locus_tag="VW852_000177"
     CDS             44599..45222
                     /gene="rplD"
                     /locus_tag="VW852_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV0271383.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            45222..45518
                     /locus_tag="VW852_000178"
     CDS             45222..45518
                     /locus_tag="VW852_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV0271384.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            45536..46369
                     /gene="rplB"
                     /locus_tag="VW852_000179"
     CDS             45536..46369
                     /gene="rplB"
                     /locus_tag="VW852_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV0271385.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            46473..46754
                     /gene="rpsS"
                     /locus_tag="VW852_000180"
     CDS             46473..46754
                     /gene="rpsS"
                     /locus_tag="VW852_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV0271386.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            46766..47110
                     /gene="rplV"
                     /locus_tag="VW852_000181"
     CDS             46766..47110
                     /gene="rplV"
                     /locus_tag="VW852_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV0271387.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            47123..47776
                     /gene="rpsC"
                     /locus_tag="VW852_000182"
     CDS             47123..47776
                     /gene="rpsC"
                     /locus_tag="VW852_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV0271388.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            47780..48193
                     /gene="rplP"
                     /locus_tag="VW852_000183"
     CDS             47780..48193
                     /gene="rplP"
                     /locus_tag="VW852_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV0271389.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            48203..48409
                     /locus_tag="VW852_000184"
     CDS             48203..48409
                     /locus_tag="VW852_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV0271390.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            48434..48694
                     /gene="rpsQ"
                     /locus_tag="VW852_000185"
     CDS             48434..48694
                     /gene="rpsQ"
                     /locus_tag="VW852_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV0271391.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            48720..49088
                     /gene="rplN"
                     /locus_tag="VW852_000186"
     CDS             48720..49088
                     /gene="rplN"
                     /locus_tag="VW852_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV0271392.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            49166..49471
                     /gene="rplX"
                     /locus_tag="VW852_000187"
     CDS             49166..49471
                     /gene="rplX"
                     /locus_tag="VW852_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV0271393.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            49495..50037
                     /gene="rplE"
                     /locus_tag="VW852_000188"
     CDS             49495..50037
                     /gene="rplE"
                     /locus_tag="VW852_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV0271394.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            50055..50324
                     /gene="rpsN"
                     /locus_tag="VW852_000189"
     CDS             50055..50324
                     /gene="rpsN"
                     /locus_tag="VW852_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV0271395.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            50609..51007
                     /gene="rpsH"
                     /locus_tag="VW852_000190"
     CDS             50609..51007
                     /gene="rpsH"
                     /locus_tag="VW852_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV0271396.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            51199..51735
                     /gene="rplF"
                     /locus_tag="VW852_000191"
     CDS             51199..51735
                     /gene="rplF"
                     /locus_tag="VW852_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV0271397.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            51819..52175
                     /gene="rplR"
                     /locus_tag="VW852_000192"
     CDS             51819..52175
                     /gene="rplR"
                     /locus_tag="VW852_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV0271398.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            52193..52687
                     /gene="rpsE"
                     /locus_tag="VW852_000193"
     CDS             52193..52687
                     /gene="rpsE"
                     /locus_tag="VW852_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV0271399.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            52659..52883
                     /gene="rpmD"
                     /locus_tag="VW852_000194"
     CDS             52659..52883
                     /gene="rpmD"
                     /locus_tag="VW852_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV0271400.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            53028..53468
                     /gene="rplO"
                     /locus_tag="VW852_000195"
     CDS             53028..53468
                     /gene="rplO"
                     /locus_tag="VW852_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV0271401.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            53481..54791
                     /gene="secY"
                     /locus_tag="VW852_000196"
     CDS             53481..54791
                     /gene="secY"
                     /locus_tag="VW852_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV0271402.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILLKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            54942..55580
                     /locus_tag="VW852_000197"
     CDS             54942..55580
                     /locus_tag="VW852_000197"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV0271403.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            55697..55915
                     /gene="infA"
                     /locus_tag="VW852_000198"
     CDS             55697..55915
                     /gene="infA"
                     /locus_tag="VW852_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV0271404.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            55940..56056
                     /gene="rpmJ"
                     /locus_tag="VW852_000199"
     CDS             55940..56056
                     /gene="rpmJ"
                     /locus_tag="VW852_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV0271405.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            56074..56439
                     /gene="rpsM"
                     /locus_tag="VW852_000200"
     CDS             56074..56439
                     /gene="rpsM"
                     /locus_tag="VW852_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV0271406.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            56457..56840
                     /gene="rpsK"
                     /locus_tag="VW852_000201"
     CDS             56457..56840
                     /gene="rpsK"
                     /locus_tag="VW852_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV0271407.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            56883..57818
                     /locus_tag="VW852_000202"
     CDS             56883..57818
                     /locus_tag="VW852_000202"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV0271408.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            57830..58216
                     /gene="rplQ"
                     /locus_tag="VW852_000203"
     CDS             57830..58216
                     /gene="rplQ"
                     /locus_tag="VW852_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV0271409.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            58481..58747
                     /locus_tag="VW852_000204"
     CDS             58481..58747
                     /locus_tag="VW852_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV0271410.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            58757..60094
                     /locus_tag="VW852_000205"
     CDS             58757..60094
                     /locus_tag="VW852_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV0271411.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAATDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            60338..61030
                     /locus_tag="VW852_000206"
     CDS             60338..61030
                     /locus_tag="VW852_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0271412.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(61133..62785)
                     /locus_tag="VW852_000207"
                     /pseudo
     CDS             complement(61133..62785)
                     /locus_tag="VW852_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(62775..63803)
                     /locus_tag="VW852_000208"
     CDS             complement(62775..63803)
                     /locus_tag="VW852_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271413.1"
                     /translation="MNITIPKGSLFTLLGPSGCGKTTLLRMIAGFNSIKDGEFYFDDT
                     KINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLKQKKVPKEELIQQTNKYLELMQI
                     AQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEPLSNLEAKLRLDMRQAIREIQH
                     EVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNI
                     IPTNLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISD
                     SVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNL
                     IKGVNHGT"
     gene            complement(63878..64999)
                     /locus_tag="VW852_000209"
                     /pseudo
     CDS             complement(63878..64999)
                     /locus_tag="VW852_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(65216..65413)
                     /locus_tag="VW852_000210"
     CDS             complement(65216..65413)
                     /locus_tag="VW852_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0271414.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(65488..66264)
                     /locus_tag="VW852_000211"
     CDS             complement(65488..66264)
                     /locus_tag="VW852_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV0271415.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            66386..67132
                     /locus_tag="VW852_000212"
     CDS             66386..67132
                     /locus_tag="VW852_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0271416.1"
                     /translation="MKRLEQIIKLVSEHEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            67145..68125
                     /locus_tag="VW852_000213"
     CDS             67145..68125
                     /locus_tag="VW852_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV0271417.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            68320..68640
                     /locus_tag="VW852_000214"
     CDS             68320..68640
                     /locus_tag="VW852_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0271418.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            68686..68994
                     /locus_tag="VW852_000215"
     CDS             68686..68994
                     /locus_tag="VW852_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0271419.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            69011..70309
                     /locus_tag="VW852_000216"
     CDS             69011..70309
                     /locus_tag="VW852_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0271420.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            70460..72898
                     /locus_tag="VW852_000217"
     CDS             70460..72898
                     /locus_tag="VW852_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV0271421.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDDRFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            72917..73585
                     /locus_tag="VW852_000218"
     CDS             72917..73585
                     /locus_tag="VW852_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV0271422.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            73603..74691
                     /locus_tag="VW852_000219"
     CDS             73603..74691
                     /locus_tag="VW852_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV0271423.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            75146..77647
                     /locus_tag="VW852_000220"
     CDS             75146..77647
                     /locus_tag="VW852_000220"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV0271424.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            77841..78536
                     /locus_tag="VW852_000221"
     CDS             77841..78536
                     /locus_tag="VW852_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271425.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            78548..78964
                     /locus_tag="VW852_000222"
     CDS             78548..78964
                     /locus_tag="VW852_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271426.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
BASE COUNT        23966 a        14470 c        17313 g        23427 t
ORIGIN      
        1 tatcttattt caatccatta tattaagaca atatgaggag aaagatgaaa gaaaaaggat
       61 tttaggaggg ggtgcaggca gtcatgccaa cggctcttgg ttatgtcagt atcggcctgg
      121 cctgtggaat tatcggtgcg ccctatgtga cacctgttga gatgggcttg atgagtctct
      181 ttgtttatgc tgggagtgcc cagtttgcca tgttggcact gattgtggtt caagctcctg
      241 tggcagctat tgctatgacg gtttttctaa tcaacttgcg tctctttttg ttgagtttac
      301 acgcatcgac ttatttccgt cataccagtc tttggtacaa tatcggtatg tctagtatct
      361 tgacagatga gacctatggc gttttgatgg gtgaattggc ccatacagac aaggtaaatc
      421 ctatgtggat gcacggaaac aatcttaaca gctatgtggc ttggtttgtg gggacagtag
      481 tcggaacggc tctgggtggc ctgctaccaa atccagaaat ctttggcttg gattttgccc
      541 tggttgggat gtttattggt atttttgctt cgcaatttca gattatgcaa agacggattc
      601 ctgtccgcaa tctgctcatt atcctagcag ttgttgcggt gtccttcttt ttgctcttga
      661 cagtggtgtc tcagtcacta gctgttctgt ttgcgacgct acttggttgt agcatggggg
      721 tggttttaga tggtcagtaa gtatctttta ttagcagtta ttttctctgg cttggtgact
      781 tggattctcc gtatgattcc cttcatctta gtcaagtata agggcttgcc tgcaatcgtt
      841 gagcgttttt tgaagttctt gcccgtttcc attatctttg ccttgattct ttcaagcgta
      901 gtgacaggta aggttgggag ccttcctcaa attaaatggc tagacttctt agccgtcttt
      961 ccaacagctt gggtagcctt tcgctaccgc aatctagtcg gtacggttct ctttggagtg
     1021 gtcttgattg ccatcttgcg tttggtcttt taaattaccc ataaaaaaaa cttatcacag
     1081 agatagatat catataatgg cgtaaatgct ctttttctgt taagattata aggtattcta
     1141 ttttggagga aatgacatga aaaaaatcgt taaatactca tctcttgctg ccctaggact
     1201 tgttgctgca ggtttgcttg cggcttgctc agggggtgct aagaaagaag gagaagcagc
     1261 tagcaagaaa gaaatcatcg ttgcaaccaa tggatcacca aggccattta tctatgaaga
     1321 aaatggcgaa ttgactggtt acgagattga agtcgttcgc gctatcttta aagattctga
     1381 caaatatgat gtcaagtttg aaaagacaga atggtcaggt gtctttgctg gtcttgacgc
     1441 tgaccgttac aatatggctg tcaacaatct tagctacact aaagaacgtg cggagaaata
     1501 cctctatgcc gcaccaattg cccaaaatcc taatgtcctt gtcgtgaaga aagatgactc
     1561 tagtatcaag tctctcgatg atatcggtgg aaaatcgacg gaagtcgttc aagccactac
     1621 atcagctaag cagttagaag catacaatgc tgaacacacg gacaacccaa ctatccttaa
     1681 ctatactaag gcagacttcc aacaaatcat ggtacgtttg agcgatggac aatttgacta
     1741 caagattttt gataaaatcg gtgttgaaac agtgatcaag aaccaaggtt tggacaactt
     1801 gaaagttatc gaacttccaa gcgaccaaca accgtacgtt tacccacttc ttgctcaagg
     1861 tcaagatgag ttgaaatcat ttgtagacaa acgcattaaa gaactctaca aagacggaac
     1921 gcttgaaaaa ttgtctaaac aattcttcgg agacacttac ctaccggcag aagctgatat
     1981 taaataattt cttgaaatca tccattctga gtaagatggg tggtttctca gtttttaggg
     2041 atatagtttt aaactatata tctgactatc taataacaat ttgtgaaatc aaacaaattg
     2101 tgagatacta gtacggtatt atttttaagg agaaagaatc atgaaaatca aaaaatggct
     2161 tggcctagca gcccttgcta cagtcgcagg tttggctctt gcagcttgcg gaaactcaga
     2221 aaagaaagca gacaatgcaa caactatcaa aatcgcaact gttaaccgta gcggttctga
     2281 agaaaaacgt tgggacaaaa tccaagaatt ggttaaaaaa gacggaatta ccttggaatt
     2341 tacagagttc acagactact cacaaccaaa caaagcaact gctgatggcg aagtagattt
     2401 gaacgctttc caacactata acttcttgaa caactggaac aaagaaaacg gaaaagacct
     2461 tgtagcgatt ggagatactt acatctctcc aatccgcctt tactcaggtt tgaatggaag
     2521 tgccaacaag tacactaaag tagaagacat cccagcaaac ggagaaatcg ctgtaccgaa
     2581 tgacgctaca aacgaaagcc gtgcgcttta cttgcttcaa tcagctggct tgattaaatt
     2641 ggatgtttct ggaactgctc ttgcaacagt tgccaacatc aaagaaaatc caaagaactt
     2701 gaaaatcact gaattggacg ctagccaaac agctcgttca ttgtcatcag ttgacgctgc
     2761 cgttgtaaac aataccttcg ttacagaagc aaaattggac tacaagaaag cacttttcaa
     2821 agaacaagct gatgaaaact caaaacaatg gtacaacatc attgttgcga aaaaagattg
     2881 ggaaacatca cctaaggctg atgctatcaa gaaagtaatc gcagcttacc acacagatga
     2941 cgtgaaaaaa gttatcgaag aaacatcaga cggtttggat caaccagttt ggtaataaga
     3001 aacagggagg tgggagagag aattccacct cttgcttttg tatagagcac ggagtgtaaa
     3061 gaagattatt cgttcataga aaggtagaga gaatatggtt tttcctagcg aacaagaaca
     3121 gattgaaaaa tttgaaaagg atcatgtagc ccagcattat tttgaggttt tgcgtacctt
     3181 gatttctaag aaatcagtct ttgcccagca ggttggactc aaggaagtcg caaactatct
     3241 gggtgagatt ttcaagcgtg ttggagctga agtggagatt gatgagagct atacagcgcc
     3301 ctttgtcatg gcacatttca agagttcgcg tccagatgcc aagaccttga ttttctataa
     3361 ccactatgac actgtgccag cggatgggga tcaggtctgg acagaggatc cttttacgct
     3421 ttcggtccgc aatggcttca tgtatgggcg tggggttgat gacgacaagg gtcatatcac
     3481 agctcgcttg agtgctttga gaaaatatat gcagcaccat gatgatttac ctgtcaatat
     3541 cagctttatc atggagggag cggaggaatc ggcttcaaca gacctagata agtatttgga
     3601 aaagcatgca gacaaactcc gtggggcgga tttgttggtc tgggaacaag ggaccaaaaa
     3661 tgccttggaa cagctggaaa tttctggtgg caataagggg attgtgacct ttgatgccaa
     3721 ggtaaaaagc gctgatgtgg atatccactc gagttatggt ggtgttgtgg aatcagctcc
     3781 ttggtatctc ctccaagcct tacagtctct tcgtgctgcg gatggccgta tcttggttga
     3841 aggcttgtac gaagaagtac aagagcccaa tgaacgagaa atggccttgc tagaaactta
     3901 tggtcaacga aacccagagg aagttagtcg gatttatgga ttggagttgc ctctcttaca
     3961 ggaggagcgg atggcctttc taaaacgttt ctttttcgat ccagcgctta atatcgaagg
     4021 aatccagtct ggttatcaag gtcagggtgt taagactatt ttacctgcag aagccagtgc
     4081 caagctagag gttcgtctgg ttccgggcct agaaccgcat gatgttctgg aaaaaattcg
     4141 gaaacagcta gacaaaaatg gctttgataa ggtagaatta tactatacct tgggagagat
     4201 gagctatcga agcgatatga gcgcaccagc cattctcaat gtgatcgagt tggccaagaa
     4261 attctatcca cagggcgttt cagtcttgcc gacgacagcg gggacaggac ctatgcatac
     4321 ggtctttgat gccctagagg taccaatggt tgcattcggt ctaggaaatg ccaatagccg
     4381 agaccacggt ggagatgaaa atgtgcgaat cgctgattat tacacccata tcgaattagt
     4441 agaggagctg attagaagct atgagtagag atattatcaa gttagatcag atcgatgtga
     4501 cttttcacca aaagaagaga accatcacag cggttaagga tgtgaccatt cacatccaag
     4561 aaggggatat ctacggaatc gttggatatt ctggagcagg aaaatcaacc cttgtacggg
     4621 tgattaatct cttgcaaaaa ccatctgcag ggaaaattac cattgacgac gatgtgattt
     4681 ttgacggcaa ggtgaccttg acggcagagc agttgcgtcg taaacgtcaa gatatcggaa
     4741 tgattttcca gcattttaac ctgatgagcc aaaagacagc agaggagaat gtagcctttg
     4801 cccttaaaca ctctgaactc agcaaggaag aaaagaaggc taaagtagct aagttgttgg
     4861 acttggttgg tttggcagat cgtgctgaaa actacccttc acaactatct ggagggcaaa
     4921 aacagcgtgt ggcaattgcg cgtgccttgg ccaatgatcc aaaaatcttg atttcagacg
     4981 agtcaacttc tgcccttgat ccgaagacaa ccaagcagat tttggccttg ttgcaagatt
     5041 tgaaccaaaa attaggcttg actgttgtct tgattacgca tgaaatgcag attgtcaaag
     5101 acattgccaa ccgtgtggcg gttatgcagg atgggcattt gattgaagag ggtagtgtgc
     5161 ttgaaatctt ctcagaccct aaacaacctt tgactcaaga ctttatctca acagctacag
     5221 gtattgacga agccatggtc aaaatcgaga agcaagaaat cgtggaacac ttgtctgaaa
     5281 acagtctctt ggtgcaactc aagtacgctg gatcttcaac agacgagcca cttttgaatg
     5341 aattgtacaa gcattatcaa gtaacggcta atattctcta tgggaatatc gaaatcctcg
     5401 atggtactcc tgttggagaa ttggtggtgg tcttgtcagg tgaaaaagca gcgttggcag
     5461 gtgcccaaga agccattcgt caagcaggtg tacaactaaa ggtattgaag ggaggacagt
     5521 aagatggaat cattgattca aacctattta ccaaatgtct ataaaatggg ttgggctggt
     5581 caggcaggct ggggaacggc tatctactta actctttata tgacagttct ttccttcatt
     5641 atcggaggct tcttggggct agtggcaggt ctctttctcg tcttgacagc gccaggtggt
     5701 gtcttggaga ataaagtcgt attctggatt ttagacaaaa ttacctcaat ttttcgtgcg
     5761 gttcccttta tcatcctctt ggcaatcttg tcaccacttt ctcacttgat tgttaaaaca
     5821 agtatcgggc caaatgcagc ccttgtccca ctttcttttg cagtctttgc cttctttgcc
     5881 cgtcaggtgc aggttgtctt ggctgaattg gatggcggtg tcattgaggc ggctcaagcg
     5941 agcggagcga ctttctggga catcgtgggt gtttacctat cagaaggtct tccagatttg
     6001 atccgtgtga cgactgtgac cttgatttcc cttgttgggg aaacagctat ggccggtgcg
     6061 gttggagctg gtggtatcgg taacgtagcc atcgcttatg gatttaaccg ctacaatcac
     6121 gatgtgacca tcttggcaac cattattatc attttgatta tctttgcaat ccaattcttg
     6181 ggagatttct tgactaagaa attgagccat aaataaaaaa gagccgcgtg gctcttttta
     6241 actgatcaga ttttctgtgc aaatttttta ctcaaggctt gtccaatcaa ggcacccact
     6301 agggctccga tgacaatact tgcgataaat agaaggacag ttccagggtt tggagcgacc
     6361 atgatacggt cgatatattc ttgggatttt cctcttgcca gaagagtagc catataggct
     6421 ttgggcgcaa tccacataag caagattggt cctgttgtac taaaggcgaa aataatgaaa
     6481 gaaaggaagt tctttgtttt gtccttgtat tttcctaaat gagctactcc atctgctagg
     6541 aggccacaga taattccagg aaggaaggaa ccagcaccgt gtttagttcc caagaaaaag
     6601 agggcaatga caaggccgat agtggtaatg gctccaaagc gcggaacttt tgcgactagg
     6661 atcatataga cgctaccgcc gacaagggca gtaaaggcag gcgcataaaa catgtttccc
     6721 gtttggtcaa agagattgcc caaaaggaca ccaatcccca tgcagaggaa gtaaagaact
     6781 gcaaaaagca gtgtagttaa gatagatttc ttcatgaatt gtctcctgat aattttttca
     6841 caattctcat tttaccatgg tttggtggga ttgtaaatgg atactagaag tttttgaaaa
     6901 cgcttgaaat atgatataat ggtttcatag ttatttttag gggaatatca tgggtagatt
     6961 tttagacttt gtctttaatc gtttcttttt agggatgatt gcgacagcct tcttttggct
     7021 attaacttta gcaggaggga ttatccttgg tctagcgccg gctagtgcca ccttgatgag
     7081 cttatatgca gaacatggtt atagctttcg ggaatacagt ttgaaggagg cttggtctct
     7141 ttacaagcaa aattttatct caagcaacct gattttctat agctttttag gtgtgggtct
     7201 agttttgacc tatggtttgt atctcttggt gcaattgcct catcagacca ttgttcattt
     7261 gattgcgacc cttttgaatg tcctagtagt tgccctgatc tttttggctt atacagtatc
     7321 tttaaaatta caagtttatt ttgccttgtc ctatcgaaat agtctcaaat tatccttgat
     7381 tggcatcttt atgagtctag cagctgtggc caaggttctc cttgggactg tgctacttgt
     7441 agcaattggt tactatatgc ctgccctgct attttttgta ggaattggga tgtggcattt
     7501 ctttatcagt gatatgttgg aacctgtcta tgaaatcatc catgaaaaat tggcgacaaa
     7561 atagaatgaa gcacttttgg ctacatacgc ttctaagaac ctatagttca gtgatgatca
     7621 ttatcattgc gagttttgca atcttactct cttacgctga ctgggattca cgtgaaaagg
     7681 aagcccagag agtagcccag cgtgtaactg ctagaacagt gagtgaaatt gaatattacc
     7741 atagagagtc aacccagata gctcaggctt tagttgaaaa tcaagctcgt attgagggaa
     7801 tctataaata ctttagcctt agcatgccag actattttta ctggcaatta gagcggaaag
     7861 cttcgcctta tatatcagtc tctctgtatg aaaatgttga tgacctctat gttcgaaatg
     7921 attttgtaac tggagtggcc attgcttttc aagattacaa ggaagtctat gtttctacta
     7981 aagacaaacg tagtggagaa aaaatcaggg ctgaggattt caaaccagca ggaaatagtt
     8041 ttgccattcc ggtgtcagat ccagtgtcag atcaagattt aggagtgatt tacatctcct
     8101 tggatcctgc tgttttatac catgccattg ataatactag aggtcatact ccgatggcag
     8161 taacagtgac ctcacctttt gatacggaga tttttcatat tggtgagaca gttgataagg
     8221 agagtgaaaa ttggctagtt ggcttaactt ctcatggata tcaggttcag gtggcagttc
     8281 ctaaaaactt tgttttacaa ggaacagtga ctagctctgc tttgattgtg ggtttgagcc
     8341 ttctctttat tgtcattctt tatctgactt tgaggcagac ttttgctaat taccaaaagc
     8401 aggtagtgga tttagtagaa tccattcaag tcattgctca aggcgaagag gggcgtcgga
     8461 ttgacatttc cgagaaagat caggaattac tcctaatcgc ggagacgacc aatgatatgt
     8521 tggatcgatt ggaaaagaat atccatgata tttaccagtt agagcttagt caaaaagatg
     8581 ccaatatgcg agccttgcag gcgcaaatca atcctcattt tatgtataat acgctggagt
     8641 tcttgcgcat gtatgcagtt atgcagagtc aagatgagtt ggcagatatc atttatgaat
     8701 tcagtagtct cttgcgtaac aatatttccg acgaaagaga gaccctcctc aaacaggaat
     8761 tagaattttg ccgtaaatac agctatctct gcatggttcg ctatcccaag tccattgcct
     8821 atggtttcaa gatagatcca gagttagaga atatgaagat tcccaagttt accttgcaac
     8881 cgctggtaga aaactatttc gcgcatggtg ttgaccacag gcggacagat aatgtgatta
     8941 gcatcaaggc tcttaaacag gatggttttg tggaaatttt ggtggtcgat aatggtagag
     9001 gaatgtcggc tgaaaagttg gcaaatatcc gagaaaaatt aagtcagaga tattttgaac
     9061 accaagccag ctacagtgat caaaggcagt ctatcgggat tgtcaatgta cacgagcgtt
     9121 ttgtgctcta ttttggagac cgctatgcca ttacgataga gtctgcagag caagccggtg
     9181 ttcagtatcg tattacaatt caagatgagt agaaagggag aaaatgtata aagtattatt
     9241 agtagatgat gagtacatgg tgacagaagg tctgaagcgt ttgattccct ttgataagtg
     9301 ggatatggag gtcgtcgcaa cagccggtca tgccgatgaa gctctagaat atgttcagga
     9361 aaatcctgtc gatgtcatca tttccgatgt caatatgcca gacaaaacag ggcttgatat
     9421 gattcgggag atgaaagaga tcttaccaga tgctgcctat atcctgctct caggttatca
     9481 ggagtttgat tatgtaaaaa gagcaatgaa ccttagtgtg gtggactatt tggtcaagcc
     9541 tgttgataag gtagagctgg gaaatctgct ggagaagatt gcaggtcagc tcggcgagag
     9601 agggaagaaa agtcagactc ttagtcaaga attagacgag gctggatttg ttagttattt
     9661 aggggataag gagaattggt ggataggtct atccaaggaa aaacaaggtt ccttcaccat
     9721 tccctactat gtcttgggtc aagactggca gattttcatt tctggccaac ccctagatgg
     9781 tttagtcgtt acaccttttg aagctcctta tcaagaacac tttgaacgct ggaagctgaa
     9841 tgctgagaaa accctctttt acggttctgt aaatttgcag cagtctgaga gtctctttgc
     9901 ctattacgaa ccgatttata gggttatcat tcagggaaat ctcaatcaaa tcgtagaaga
     9961 gttaaatctc ttggagaagg tagttcttga aaatacgccg cgtgttccga ttactaaaca
    10021 gctttttatt cagtttgtca tggatgtttt ccatttattt gaacatctca aagctgatga
    10081 cctgacagac atcgtcaaaa ccattcatgc tattcaatcc ttcgatgaat tggttcctta
    10141 tatcaaggaa actctgacca gctttttcgg tcaataccgt atgaatgaaa atgtggccag
    10201 tgtgctggaa gtcattggtc gtgattacca aaaagagctt tccctcaagg atatcagtaa
    10261 ggccctcttt atcaatcctg tctatctagg gcagttgatt aagcgtgaaa ccgattcgac
    10321 ctttgcagag ttactaaaca aacaacgtat taaggctgcc caacaacttt tgctttcaac
    10381 tagtgacagc atcgaagata tttgttatgc tgttggttac agtaaccttg gatatttcta
    10441 taaagttttc cgaaaattgt gcggaaaatc gccaaaagcc taccgaaaac aggtagaaac
    10501 tatactataa gatttgtatt cctttacaaa aggtgctata atattatcag gaggtcctat
    10561 catgaaaaag aaaccaattt atctatgggt cttgctaatc ttgtctgccc ttatttcagt
    10621 tccgtctctg tttggaattg tgagtccctt gcctagtaaa gaagcccttc gtgctgctca
    10681 gaaacaagtt gcaggggtca atgctcagca attagaagac cagcttaatt atacctatag
    10741 agtagcagaa gcatctcatt ctatttttaa tgttgctttg attgtgctat ctactatttt
    10801 agtggtcgta gcgattgtct tccttgttcg taaaaatttg caatatgcaa actatactta
    10861 cgttggctat gttttgttag ctattattgg ttcgatttat ggctatgttg gtttacaaga
    10921 cgctgtgcag ttggttcaag atgagagcat gcgtttgaca gtgagtattg gctcaaaagc
    10981 tgttagcatt ttctatatcg tcatcaatgt tctcttccta gcccttgtct tctataagat
    11041 gtggcgacaa cagaaagcct tggcagaaga agaggaaaca gaagagcttg cctaagtatt
    11101 gacaaaaaag aactatcaag atacaatctt ggtagttgtt ttttgattat agataaaatc
    11161 atggaggtac atatgaagac aatttcttta gtttatatta gtttgagtgg caacactgaa
    11221 agttttgtga cgcgcttgaa agactatctc ttgtcccagt acaaagggat tgaggttcaa
    11281 aagattcata tcaaggattt ggtcaaggaa ggcaaaaatt tctatgaaat ggaccatccc
    11341 tatgtcgctt ttttgccgac ctatctcgaa ggtgggaatg gcgtggataa cggagatgtt
    11401 gagattttga cgacaccggt gggagatttt attgcctatg gtaacaatgc tagtaagtgt
    11461 tttggtgtgg ttgggtcagg aaatcgtaac tttaataacc aatactgcct aacagccaag
    11521 caatacagtc aacgctttgg cttccctgta ttggctgact ttgaaatgcg aggcatgctg
    11581 gaagatatca aacatgtcgc agctattatc gcagatttgt atgagttgga aaaggagaat
    11641 taatactcaa tgaaaatcaa agagcaaact aggaagctag tcgcaggttg ctcaaagcac
    11701 agctttgagg ttgcagatag agctgacgtg gtttgaagag attctcgaat agtataaagt
    11761 tcttataaga ggtggttgaa gttgttcaac tgttttttga gtataaacag tctttaagaa
    11821 cgtaaaaaag cataagttca ggtcttcaaa accttgttct tatgcttttt atcatagaaa
    11881 tatttgctga attttctctt gaaatgaaac cttatcttca tctcgcgctt gtcccttacc
    11941 ctgcttttcc gattgcgagt gcgtagataa agaagatggc gaagccaaag aggaaaatca
    12001 atcccgcaat ggcaaggtgt ttgagccaag aaggaagatt tttgttttca cgcgttacca
    12061 aggcgtaatt caaaagagca aagaaaggtg ttgtcaggaa agatccaatc atggcaaagc
    12121 ggagcatggt tgaaacctga ccagcgaaga acttgataat gacgataccg atgatagcag
    12181 tgatggtcat ccagatgttc aaagatttac gattgtcctc tttttgactg attagcagtc
    12241 ggagagattc ctgattaacg cgagaatagc catcgataac agttataact gttccaaaga
    12301 tacagaggaa ggcaataaag gtaatcaagt aacgggacca ttcgccaaga acagaggcat
    12361 acatgcccac gaattgagag atgtatttgg ctgaagcagc ttcaaccgcc tgccctgtag
    12421 gatattgaat cagtgctccc agtgccacaa agaagacggc taggatagtt gttccaatat
    12481 aaccagtgtt aaagtcaaac agagcgtctt ctgtgttaaa gttgacggtc tttctctttt
    12541 cagctgacca aagtgaattg atggctgaaa tttcaatagg agccggcatc catcctagga
    12601 gggagacgat gaagggcaga gctgccattt gccaaggtgt cttctcgaca aaatcagaac
    12661 tgtattctgg atgcttgacc gccgcaatga taactgccag aacagtcgca atggttaagg
    12721 cagacataat ccatttgacc atgccgtcta aaagtttgta gcctccaaag agtagcatag
    12781 cccaaatgat tgcaacgaga atgagggacc actgagtaat gctaagtcca atcattggga
    12841 aggcactggc gatgatagct gagcacagaa tggcaacacc agccgtgttg accatagccg
    12901 aaaagacatt gaggataaag aaaatccaga gatagagttt tcctttttcg gcataacctt
    12961 caaccaaagt ctttccagta tcagctgtgt attcagcacc aaaacggaaa aatggatatt
    13021 taaagacatt ggctaagatg accaagagaa gtagagacca accgtaagaa ccgccagctt
    13081 gagttgagga tacaatgtgg gaacctccaa cagcggcagt tgccattagg attccaggtc
    13141 ccattgcttt tagcttgctt gcccaggttg attgatttaa ggaaatagct tgcgacatga
    13201 taaatactcc tttttgaatt ttcagaataa tctgtatatg taatctatat tctacaaaaa
    13261 attttggaaa aatgcaagag tattttgaaa aaagatagaa aatttacaaa gaagatctga
    13321 aataactgtt atgatatagc ggattgaaac tagaatagta cacctttgct tctaaaacat
    13381 tgttagaaat cgatttgact gtcctgatcg atttatcctg ttcttatttc atttcactat
    13441 agtaaggtgc agcaggataa agaaaaaccg agaagtttct ttctcggatt tgcttattat
    13501 gaggtgccta ttttctatgc tccaagagtt gattgatgtg atctactttt ttagcctctc
    13561 ttttatagag aatagcccag atgatgagat agacaatcgc aaactcgata atgagctgga
    13621 ggtagaaaat ccagtggaag gggaaccaac cagcaagagt tgctagtggg acaaagccag
    13681 ccagcataag gaagaaatga gtcagagtgg cacgaagcat gctccagtca cggctgaata
    13741 agcggttgcc aaagttgaag agcataccga tggctgccca gataagtgtg cagtagagca
    13801 agaccagggc accgtgaacc tgatgctgat ccatcacttg gccgatgaga gagtagggat
    13861 ttagtggtgc gtaggtattt ggtgcataaa tgagtgaaaa gaggatggag aggatgaggc
    13921 cgataagaac accggcagct gcatcgtgaa atacttgttt tttcatagtt ctaatttctc
    13981 cttgatggtt tttagataac ggcgtgaaga gtaggtgaag ctttcgtttt tcaagaaaat
    14041 ttctaccaga ccgtttggcg tgagcttgag gtgagagatg gaatcgatat tgatgatttc
    14101 tgattgggaa atttggataa aattgcttgg caagagttta agaacctgat agagtcgcaa
    14161 atcaatgctg taggtctgac tcgcggtttc tgctagaacc ttccgattct cgatatagaa
    14221 gcgctgaatc ttgccaatct caactagata gacctgatca tcgatttttc ctttgatttt
    14281 ttctctttgg tccagatttt ctgcgaactc gatgactttc tggacttttt cggtttcttg
    14341 aggtgcttgg acaatcagct tttcctcctc gtaagtctca ctaatctgta gttctacttt
    14401 cataaatttc tctccttgtt tttcatacaa aattaatctc acttcttgta cacctttatt
    14461 aatactcttc aaaaatctct tcaaaccacg tcagcgtcgt cttgccgtag gatagttgtt
    14521 gactttgtca gttttatcta caacctcaaa acagtgtttt gagctgtctg cgcctagctt
    14581 tctagtttgc tctttgattt ttattgagta taccactatt ttactccctc tggcaaggga
    14641 ctttgtctat gtggagggat tgggctccta tgtggtggag cttttctgtt ctttctgaaa
    14701 tatggtataa tagcactaat taatttctag gaaaatagat acagaaaggg gctgaaagat
    14761 gtctcatatt attgaattgc cagagatgct ggcaaaccaa atcgcagctg gagaggtcat
    14821 tgaacgtcct gccagtgtgg tcaaagagtt ggtagaaaat gccattgacg cgggctctag
    14881 tcagattatc attgagattg aggaagctgg tctcaagaaa atccaaatca cagataacgg
    14941 tcatggaatt gcccacgatg aggtggagtt ggccctgcgt cgccatgcga ccagtaagat
    15001 aaaaaatcaa gcagatctct ttcggattcg gacgcttggt tttcgtggtg aagccctgcc
    15061 ttctattgcg tctgttagtg tcttgactct gttaacggcg gtggatggtg ctagtcatgg
    15121 aaccaagtta gtcgcgcgtg ggggtgaagt tgaggaagtc atcccagcga ctagtcctgt
    15181 gggaaccaag gtttgtgtgg aggatctctt tttcaacacg cctgcccgtc tcaagtatat
    15241 gaagagccag caagcggagt tgtctcatat cattgatatt gtcaaccgtc tgggcttggc
    15301 ccatcctgag atttctttta gcttgattag tgatggcaag gaaatgacgc ggacagcagg
    15361 gactggtcaa ctgcgccaag caatcgcagg gatttacggt ttggtcagtg ccaagaagat
    15421 gattgaaatt gagaactctg acctagattt cgaaatttca ggttttgtgt ccttgcctga
    15481 gttgactcgg gctaaccgca attatatcag tctcttcatc aatggccgtt atatcaagaa
    15541 cttcctgctc aatcgtgcta ttttggatgg ttttggaagc aagcttatgg ttggacgttt
    15601 tccactggct gtcattcaca tccatatcga cccttatcta gcggatgtca atgtacatcc
    15661 aaccaagcaa gaggtgcgga tttccaagga aaaagaactg atggcgctgg tctcagaagc
    15721 tattgcaaat agtctcaagg aacaaacctt gattccagat gccttggaaa atcttgccaa
    15781 atcgaccgtg cgcaatcgtg agaaggtgga gcaaactatt ctcccactca aagaaaatac
    15841 gttctactat gagaaaactg agccgtcaag acctagtcaa actgaagtag ctgattatca
    15901 ggtagaattg actgatgaag ggcaggattt gaccctgttt gccaaggaaa ccttggatcg
    15961 attgaccaag ccagcaaaac tgcattttgc agagagaaag cctgctaact acgaccagct
    16021 agaccatcca gagttagatc ttgctagcat cgataaggct tatgacaaac tggagcgaga
    16081 agaagcatcc agcttcccag agttggagtt tttcggacaa atgcacggga cttatctctt
    16141 tgcccaaggg cgagatggac tttacatcat agatcagcac gctgctcagg aacgggtcaa
    16201 gtacgaggag taccgtgaaa gcattggcaa tgttgaccag agccagcagc aactcctagt
    16261 gccctatatc tttgaatttc ctgcggatga tgccctgcgt ctcaaggaaa gaatgcctct
    16321 cttagaggaa gtgggcgtct ttctagcaga gtacggagaa aatcaattta ttctacgtga
    16381 acatcctatt tggatggcag aagaagagat tgaatcaggc atctatgaga tgtgcgacat
    16441 gctccttttg accaaggaag tttctatcaa gaaataccga gcagagctgg ctatcatgat
    16501 gtcttgcaag cgatctatca aggccaatca tcgtattgat gatcattcag ctagacaact
    16561 cctctatcag ctttctcaat gtgacaatcc ctataactgt cctcacggac gtcctgtttt
    16621 ggtgcatttt accaagtcgg atatggaaaa gatgttccga cgtattcagg aaaatcacac
    16681 cagtctccgt gagttgggga aatattaaaa gtataaaaaa gtctgggaaa aattttcaaa
    16741 atcagaaaaa cgcataaaat caggtgttca aaaaccttga ttttatgcgt tttatcatag
    16801 aaatatttac ttcatgtttt cctaattctt ttcgaaactc tttttaaacc acgtcagttt
    16861 tatcagtaat ctcaaaacag tgttttgagc taattttgcc agttttgtct gtaacatcga
    16921 agttgtgttt taccactctg cgactggttt cctagtttgc tctatgattt tcacagagta
    16981 ttaaattgcg attttgccaa gtttctttat tcgtctaaaa gtagagtctg ttctatgcgt
    17041 ctaatgtacg aattaggttg accatttcaa tagctccttg tgcacactca gaacccttat
    17101 ttcctgcttt ggtaccagct cgttctatgg cttgttcaat tgtatctgtc gttagcacac
    17161 caaacataac aggaatttcg ctatttaaac tgatttgggc gattccctta gatacctcgc
    17221 tacatacata atcataatga cttgtattcc ctctaatgac agctcccaag cagataattg
    17281 catcatattt tttacttttt gccatttttg atgcaatcag tggtatttca aaagctcctg
    17341 gaacccaggc tacctctata tctttctcgt ttacattctc tcttttgaga ttatctagtg
    17401 ctccagataa taattttgaa gttataaatt cattaaatct cgctacaaca atacctattt
    17461 taatattgtt tgctactaaa ttaccttcat aagtgttcat ttatttttcc tccatattta
    17521 aaatgtgacc cattcgattt ttctttgttt ctaaataaaa actatcgtaa ggattggctt
    17581 ctatttcgat tgatattcta ctggaaatgg taattccata tttttctaac tgttcaacct
    17641 tgtcaggatt atttgtcagt aaatgaagtg actgaagtcc cagatcttta agcatttgtg
    17701 ctccaatatg atattctctt aaatcacctt caaagcctaa tgcaagattg gcatcaagcg
    17761 tatccatgcc ttgatcttgt aaatgatagg cttttaattt attgataagt ccaattcctc
    17821 gtccctcctg tcgcaagtaa agtaagacac ccgaaccatt ctcaacaatc attttcatag
    17881 ctttatcgaa ttgctgtcca caatcgcaac gtaaagagcc taaaacatct cctgttaaac
    17941 attcggagtg gacccgacat aatacattgg cttcatcctc tatgtttccc ataataagag
    18001 caagatgatg ttccccattt agtttatcta tatagctaat tgctttgaaa ttaccgtatc
    18061 tagtaggcat attgacagtt gaaactcgtt ctaccagctg atcatatact tttctatatt
    18121 cttgtaattc tttgatggta attagtggaa tgttgtgttt cttcgagaac tgaattaaat
    18181 catctgttct catcattttg ccatcatgat tcattatttc acaacatagg ccacactctt
    18241 ttagtccagc taattttaat aaatcaacag ttgcttctgt gtgtccattt ctttctagaa
    18301 caccaccttt ttttgcaatt aaaggaaaca tgtgtcctgg cctgcgaaaa tcagagggtg
    18361 ttatatcttc agctacacac atacgtgcgg tcagtcctct ttcctcggca gaaatacctg
    18421 tggtcgtttc tttataatca attgaaactg taaaagcagt cttatgatta tctgtattgt
    18481 tttcaaccat aggtgaaagc attaattgat tagctaaact ttcgctcata ggcatacaaa
    18541 ttaatccttt ggcataagta gccataaaat taacattttc tgttgtagct gcttgtgcag
    18601 aacaaattaa gtctccttca ttttctctat ccttgtcgtc tataacaaga acaagtcgtc
    18661 ccttctgcaa tgcttctaat gcttcttgta tttttcgata ttccattgac tgattatcct
    18721 ttctgctaaa atccattttg atataatagt tccttagata tttctgattt tggagagtta
    18781 tccatcagtt tttgcacata tttacctaag atatcatttt caagatttac tgtactcccg
    18841 acttgtttac tcttaagaat ggtttgttcc aaggtatgag ggataacaga tactgaaaag
    18901 tttactttgg agactttagc gacagtcaga ctaatgccgt caattgtaat agatcctttt
    18961 tcaactatta aatctaaaat ttctttttgt gtgttgattt gataccatac agcattatca
    19021 tcttttttta ttgacgagat ttttcctgta ccatcaatgt gtcctgtaac gacgtgaccc
    19081 ccaagtcgac cgttgacaga taaggctctt tctagattca cctcacttcc atgttttaat
    19141 agagtaagag ctgttcgact ccatgtttca ttcattacat caactgtaaa ggattgatga
    19201 ttgaaatgag taactgtaag acagatacca tttactgcta tactatcgcc taaatggata
    19261 tccgttaata tttttgaggc tttaattgat agtttacaat tacgagagtc tttctgtatt
    19321 ctttcaactt ttccgatttc ttcaattatt cctgtgaaca tggataaatc acttcacttt
    19381 ctatgagata gtcatttcct atttgagaaa aagcataagg tttcaatcta atagcgtcat
    19441 ttggcaaaga aatgccttca cctccgacag gaaacttggc actgcctcca aaaatttttg
    19501 gtgcaatata tattttcagc tcatcaacaa tttgttgttc caaagcactc caattcatta
    19561 gactgccccc ttctagaact aggctatcaa tctgcatgtt tcctagatgt tgcattaaac
    19621 tcgataagtc tatatgattg ccttttttct ttatggaaag tatttcacag ccatgatttt
    19681 gatatagctt cattttattt ttgtcttcag aggaagtggc aatgtaagtt ttaatatcat
    19741 ttgctgtttt tacgatttta gaggtaagag gagttcgtaa atgtgtatcg catatgatac
    19801 gaataggatt tttcccttcc tccaatctac atgtcagcaa aggatcgtct tgaataacag
    19861 tattgactcc caccataatt gcactaacat ggtgtcgtaa ctgatgcaca tgctttcttg
    19921 cttcttcttc agtaatccat ttggattgat ttgttttagt ggctattttt ccatccattg
    19981 acattgcata tttcataaaa acatagggta catgctgggt aatatacttt ctaaaacttt
    20041 ttattaagtt aagacactca ttttctaaaa ttccaacagt aacttgaaga ttattttcct
    20101 caagtatctt tactcctttt ccagatacaa taggattaca gtctaggctt ccaatgacta
    20161 ctcttgtaat accactatcg attatagcat ctatacaagg aggtgttttc ccgaagtgac
    20221 aacagggttc aagtgttaca taaagcgtcg ctccgacagg ggattctcta cagtttttaa
    20281 gagcatttct ctcagcatgt gagccaccaa aaaactcatg ataaccttgt ccgatgatgt
    20341 gattatcttt tacaataact gcgccgacca taggattggg attgacgtaa ccagcccctt
    20401 tttgtgccag ttttattgct aatttcatat attttgaatc gctcatctca ctacctccaa
    20461 aaaaatatac cttgaatagg agactactca aggcatacaa aagaaaactt atgcgattaa
    20521 caaaaatgct ctgaaatgac aagtaatcat ttcagagcac gcaaaaagca caaatatact
    20581 tttatcttct ttcatccaga ctatactgtc ggctttggaa tttcaccaaa tcatgccttt
    20641 cggctcgtgg gctataccac cggtagggaa ttgcaccctg ccctgaagat agttattcaa
    20701 ttacagatga ttatagtact taattttgaa tatgtcaaca gataaatacc gattgttttt
    20761 gatatactgt atttgtgata atcgattctc gctcctcgga taaagaaaat atgatatact
    20821 agataaacga aataagagag aaggaacact atgtacgcat atttaaaagg aatcattacc
    20881 aaaattactg ccaaatacat tgttcttgaa accaatggta ttggttatat cctgcatgtg
    20941 gccaatcctt atgcctattc aggtcaggtt aatcaggagg atcagattta tgtgcatcag
    21001 gttgtgcgtg aggacgccca tttgctttat ggatttcgct cagaggatga gaaaaagctc
    21061 tttcttagtc tgatttcggt ctctgggatt ggtcctgtat cagctcttgc tattatcgct
    21121 gctgatgaca atgctggctt ggttcaagcc attgaaacca agaacatcac ctacttgacc
    21181 aagttcccta aaattggcaa gaaaacagcc cagcagatgg tgctggactt ggaaggcaag
    21241 gtagtagttg caggagatga ccttcctgcc aaggtcgcag tgcaagcaag tgctgaaaac
    21301 caagaattgg aagaagctat ggaagccatg ttggctctgg gctacaaggc aacagagctc
    21361 aagaaaatca agaaattctt tgaaggaacg acagatacag ctgagaacta tatcaagtcg
    21421 gcccttaaaa tgttggtcaa ataggagcag agaatgacaa aacgttgttc gtgggtcaag
    21481 atgaccaacc cgctctacat cgcctatcat gatgaggagt ggggtcagcc cctccatgat
    21541 gaccaagcat tgtttgagtt gttgtgtatg gaaacctatc aggcaggcct gtcttgggaa
    21601 acggtactca acaaacgcca agctttccga gaagcctttc atagctatca aattcactca
    21661 gtcgcagaga tgactgacac tgaattggaa gccatgctgg agaatccagc tatcattcga
    21721 aatagagcca agatttttgc tacacgcgct aacgcccaag cctttctaca gttacaggca
    21781 gagtacggct cttttgatgc ctatctttgg tcttttgttg aggggaaaac tgtcgttaac
    21841 gatgttcctg attatcgcca agccccagct aaaacaccct tatctgagaa attagccaaa
    21901 gatctcaaaa aacgaggctt caagttcaca ggcccagtcg ccgtattgtc ttttctacag
    21961 gctgcagggc tagttgatga ccacgagaat gattgtgagt ggagaggtct taaatgatgt
    22021 ctaacaaaaa taagggaatt ctgatttttg cgattctcta tacagtcctc tttgtgtttg
    22081 atggcgttaa attgctggct tctttaatgc catctgccat tgcaaattat cttgtttatg
    22141 tagttttagc tctatatggc tccttcttat tcaaggatag attgatccaa caatggaagg
    22201 ggattagaaa gactaaaaga aaattcttct ttggcgtctt aacaggatgg ctctttctca
    22261 ttctgatgac tgttgtcttt gaatttgtat cagagatgtt gaagcagttt gtgggactag
    22321 atggacaagg tctaaatcag tctaatattc aaagtacctt tcaagaacaa ccactactga
    22381 tagctgtttt tgcttgtgtc attggacctc tggtagaaga attatttttc cgtcaggtct
    22441 tattgcatta cttgcaggaa cggttgtcag gtttactaag cattattctg gtaggacttg
    22501 tttttgctct gactcatatg cacagtttgg ctctatcaga gtggattggt gcagttggtt
    22561 acttaggtgg aggccttgcc ttttctatta tttatgtgaa agaaaaagag aatatctact
    22621 atcccctact tgttcacatg ttaagcaaca gcctctcctt aatcatttta gctatcagta
    22681 tagtaaaatg aaatgagaac aggacaaatc gatttctaac aatgttttag aagtagaggt
    22741 gtactattct agtttcaata tactgtaata tgtgatgaaa atgccagtaa tgataccgag
    22801 aaaaaagctg agaaactttt cccagcttta tttgttatag tcaaagagaa tgacttgttc
    22861 ctgtgcatct acatgagcat ggaccccaaa gggtacaatt gctcttggag ttgcgtggcc
    22921 gacattcaga ttatagacaa tcgggatatt gctgtcaatg atatccaata gtgcctcttt
    22981 atagtcgtca tggaaagttt catccatagg ttttccgacc aagagtccat tgatgaccgc
    23041 gaatatgcca gtgtccttta aagttagcaa catctttttg aagtcttctg gcttaggctt
    23101 ttcttcgctt gtttcgagca agaggatttt cccttcccag tctgacaagt cagggaaaag
    23161 tttgtatttt tggcagagct ccgtgctatc tgcgtatcga gagttgtcaa agatatcgta
    23221 gagggattcg aggcaaccac cgaggatttt cccctcgaac tgggcacttc cttgcaacaa
    23281 gtcaaaacct gtatttgtat gactgacacg aggtgttccc agggccgtgg gactaaaatc
    23341 agttcgttcc tcataccaaa cgtcactagg gcggatttct gaaattcttc ccgtctcaat
    23401 caattcttta aagtagtgaa ggctataggc tagcatttct ttgtctaatt cacaaatgtc
    23461 tgctaaaaag gattgaccat aaaaagtctt gatcccaaat ttcaagttga agttagccac
    23521 ccaaaagaaa ttcacttggc gacttgtagt ccaagcattt tttagggtag ttgttaatcc
    23581 aattttcgat aaaagctact tctttcggag tcgttttctt ggttccttta ggtaaccatc
    23641 tccgaattaa tcgattatga ttttcatttg aacctctctc ccatgaagag taagcatgtg
    23701 cgtagtagat atgttcctca ggaaatacct cagacaatcg tttgaactcc gaaccattat
    23761 ctgcagtaat ggacagaata cgatgctccc ctaatagtaa cgtaagcgcc tgattgacag
    23821 attcagcagt tttatttgga attaacctta taatttggtg tctgctccgc cggtcggtta
    23881 agactaacag gcaataattt tttatcttag tcagtaggac ggtatcaatt tcataatgac
    23941 cattttccaa gcgaagatta ataacgtcag gacgttcttc gatagattta cctgccggct
    24001 taaagttcgg actagcttgc ttcttgacac ctttcctttt tctgggataa agcatgtcgg
    24061 ccttcgtcaa tcctaaatga ccattatgaa accagtagta gatggttgaa ataccaactt
    24121 tcacttgctt cttgttaacc atcatttcag gcgaaaattt ttgcttatga tagtgtaaga
    24181 tcttctctct gatttccttt gttaaaatta actttttcac cgaccgtttt cgattgaatt
    24241 ggtaaacagt ttgtgcgtaa tcggcagaat agaccttttt gtatagccct tttctcactt
    24301 gttgtaaagt tgtacctctt ttgacttcat tatgaatcgt ttgaggcgcc tttcctaaca
    24361 agcctgcaat ttcacgattg gacttatttt cattcttcca ccgctcaatc aagcgacggt
    24421 tatctattgt caaatgtttc ccttttgggg tataatgttc ttgcatctct gtgcctttcc
    24481 ttgtgtttgt ggtgaacaac aagtatagca cagaggtgtt ttcttatacc ttaaatcaca
    24541 tttcatttga caacaggaac ccttgaacct gttttttcag ctaaaccaag gctatgatta
    24601 gccttggcca cctagcctaa cagtttcaaa cctgttatca aatgaaatca tcaacttact
    24661 caaacatagt gaaacatcta attcttgact aaggtaaggt ggctaacttc attatagaac
    24721 tttcataaaa agtcttgatt cctaatttat gcaacatgag gtggttcatg gttgtatccg
    24781 agaagccaag aaaaattttt tgtttgataa ccttttggag ttggtcattt tcaaaaagat
    24841 aaggtagcaa gcgataggta tcgtctccac cgatggcaca taggatcatg tcgatgctat
    24901 catcagaaaa ggcatgaatc aaatcctctg cacgagcttc aggatggtcc ttgataaagt
    24961 ctaatccttt taacgaatgg ggcaaaaaga tgggattgag tctcagatcc ttgagacgtt
    25021 ggatacccaa gtccacttcg tgtttgacaa aatcctctcc gataatgcca ctagataaac
    25081 taacaatacc aatagtagaa accatatctc atcctcctag aaatagattg agcctatttt
    25141 atcacaaaat ataagagaaa gctatgtggg atttcagaag aatactttta atactcttcg
    25201 aaaatctctt caaaccacgt cagttttatt tgcaacctca aagctgtgct ttgagcaacc
    25261 tgcagctagc ttcctagttt gctctttgat tttcattgag tataactcaa aaaagtaagt
    25321 gaaaaagcaa ccgcacctgc agttgctttt atcgttttct tattcttgat agcgtgttgg
    25381 tcctgcactt gcactacgga tattcaagcg tttcttgaga tagaagcgaa gggctaggag
    25441 ggctgctcca ataatagcta gtggcaatgg atccagtact gggttaaggc tagctggtag
    25501 gaagcttgtt gcaaagaaga caagcaacca aaggaacata gaagctagga taactagtac
    25561 agatttccag aaaggtggac gttgactgcg atccatattt ggtccatagt attggtaaac
    25621 aaagtagtac atcaagtaga aggcaaatcc accaaccagt ccaactaata gaagagtaat
    25681 caatccatag ccgaaagctt gatctgccgc aaagaaggtt gtgagggcgc tgacaagggc
    25741 aaagaggcta gtgatgaaaa gagctgagtc cataatcatc agttttggat catcattttc
    25801 ttttggatgc tctttttcgt actgctcttt gacagtgaag ctatgagccc aatgagttgg
    25861 tgcgccgtat agggaacggg cagttgtacc tttagattgc tcctcaagga tttggggaat
    25921 gacttcctca aaaatagtct ggatttcagc gtctgttttc ccatctttga tgaattgttg
    25981 ggtagcaatg tggacaaact cttggttttt cttagttaat ttttgtagat caatttgaga
    26041 cataggaact cctcttagaa ccatttttta tggatgagat agagagtgag cgagacactc
    26101 atagcaaagg cgataaagac gattaaccag aaggcatttg gctctccgtt tagggggatt
    26161 tcattatcct taaagttcat cccgtaggca gaaaagacca tggttgggat ggacatgacg
    26221 atggtcacaa gggccaaggt tttcatgatg ttgttctggt tgttagaaat gatagaggca
    26281 aaggtctctg tcatagaatg caagacgttt ccataaatat ctgccatctc gatggcctgt
    26341 tgggtttcaa tcagggtgtc ttcaagcagg tcttcgtcct caaggtattt cttgatattg
    26401 ctggttgaac tggtcaattt cttaatcacg cgctcatttg ttttgaggga ggccttgaaa
    26461 tagacgatag ttttttccaa ttccatgagc tcaatcaatt cttcattacg agttgattga
    26521 tgcagttgac tttcgatttg ttcactcttg cggtcgattg aacgaagggc tgttaggtaa
    26581 agctctgcat tgcgataaag aatttgaaag ataaaacgtg aacgcatgaa ggtatagaaa
    26641 ttacgcaatc gacggttgat aaagacatca aggacaggta gtggttccaa acacgtagtg
    26701 ataatggttt cctcagtgat gataatacca agcgggatgg ttacgtagta ggtgcggtta
    26761 tttctttcct ccgtgaccgg cacgtctacg ataatcaggg tatactcgtc ttcaatggta
    26821 atacgagaca tttcttccgc atcgagcggt gctcgaaggt cggcaatatc aatatcgaag
    26881 gtgttggcga tttcgagtga ttcattttga gtcggattga cgagattgat ccaagtaccc
    26941 ggttcaagcg tatcgatctc tttaaattca gttgttgtag agagaaaaac ttgtttcata
    27001 tcccttatcc tttctcattt ttcagatttt ttcacactgt actattatac tacaaaatcg
    27061 gccttttggg taatagaatc tcactttttt tggtataatg gtaagcaata atggactaga
    27121 aagaacaaag atgcaagata aaattgtcat tcatggggcg cgtgcccata atttaaaaaa
    27181 tattgatgtg gagattccgc gagacaagtt ggttgtcgtg actggcttgt caggttcagg
    27241 gaagtccagt ctggcttttg atactctcta tgcggaggga caacgtcgct atgtagagag
    27301 tttgtctgcc tacgctcgtc agttcttggg aaatatggaa aaaccagatg tagatgccat
    27361 tgatggtctc agcccagcta tttccatcga ccagaaaacg actagtaaaa atcctcgctc
    27421 gacggtggga acgacgactg aaatcaatga ctatctgcgt ctcctctacg cacgtgtggg
    27481 gacgccttac tgtatcaacg gacatggagc tatcaatgct tcttctgtgg agcaaatcgt
    27541 tgataaggtt ttggagttac ctgaacgcca gcgcttgcag atcttggctc ctgtcatccg
    27601 caagaaaaaa ggccaacata agagtgttat cgagaaggtt cagaaagacg ggtatgttcg
    27661 tgttcgtgtg gatggggaag tctatgatgt gaccgaagtg ccagagttgt ctaagagcaa
    27721 gcaacacaat atcgatgtcg tggttgaccg tattgttatc aaggagggca ttcgtagccg
    27781 tctctttgat tccattgaag ctgcccttcg tatcgcagaa ggttatgtga ttatcgacac
    27841 catggacgac tctgagttgt tgttctctga gcattatgcc tgtccagttt gtggatttac
    27901 tgtcccagag ttagaacctc gtctcttctc tttcaatgct ccctttggct cttgtagtga
    27961 gtgtgacggc ttggggatta agctggaggt ggatactgat ttggtagtgc cagatgccag
    28021 caaaacctta catgagggag cgctggcacc ttggaatcct atctcatcca actactatcc
    28081 aaacatgtta gagcaggcca tgaaagtctt tggagtggct atggataagc cttttgagga
    28141 cctgtcagaa gaagataaga acttgattct ctacggctca gatggtaagg aattccattt
    28201 ccattatgaa aatgaatttg gtggtgtgcg cgatatcgac attccgtttg agggagttat
    28261 caataatatc aaacgtcgtt accatgaaac caatagcgat tacactcgca ctcagatgcg
    28321 tctctatatg aatgagctga cctgcggaac ctgtcaaggc tatcgtctca atgaccaggc
    28381 cttgtctgtc cgtgtgggcg gccagcaagg gccacatatt ggagaaatct cagacctgtc
    28441 tatcgctgac cacttggact tggtgagcca gttaactttg tctgaaaatg aagccatcat
    28501 tgctcgtccc attctcaagg aaatcaagga tcgtttgacc ttccttaata atgtgggtct
    28561 taactatcta accctgtcac gttcagcagg aaccctttca ggtggggaaa gtcagcgcat
    28621 tcgtttggca acccagattg gttccaacct atcaggcgtc ctctatatcc tagacgagcc
    28681 gtcaatcggt cttcaccaga gggacaatga ccgcctgatt gccagtctga aaaagatgcg
    28741 tgacttgggc aatactctta tcgtggtgga acacgacgaa gataccatgc gtgaagctga
    28801 ttatctgatt gacgttggtc ctggtgccgg tgtttttggt ggtgagattg ttgcagcagg
    28861 tacacctaag caagtggctc gcaacagcaa gtctatcaca ggtcagtact tgtcaggcaa
    28921 acgtgtcatc ccagtaccag aagagcgccg tgtcggaaat ggtcgtttta ttgaagtgat
    28981 aggagcgcgt gagaacaact tgcaaaatgt cacggctcgc tttccactag gaaaatttat
    29041 cgcagtgaca ggtgtgtcgg gttcagggaa atcgactcta atcaacagca ttctcaaaaa
    29101 agccattgcc cagaagctca accgtaattc agacaaacct ggtaagttta agactattac
    29161 agggattgag catgtcgacc gtttgattga tattgaccag agccctatcg gacggacgcc
    29221 gaggtctaac ccagctacct atacaggagt ttttgacgat atacgtgacc tttttgctca
    29281 gacaaatgaa gccaagattc gaggctacaa gaaggggcgc ttcagtttca acgtcaaggg
    29341 aggtcgctgt gaagcttgct caggtgacgg gattatcaag attgagatgc acttcttgcc
    29401 agatgtttat gtggcttgtg aagtttgcca cggaacccgc tataacagtg aaaccctaga
    29461 agttcactac aaggaaaaga atatttcgca ggtcttggat atgacggtca acgatgcggt
    29521 agaatttttc caacacattc cgaaaattca acgcaaactc cagaccatca aggatgtagg
    29581 gctaggctat gtgaccttgg gacagccagc taccaccctt tctgggggag aagcccaacg
    29641 tatgaagtta gctagcgaac tccacaaacg ctcgacagga aaatctttct acattctgga
    29701 tgagccgacg acagggcttc atacagagga cattgctcgc ctgctcaagg ttttagctcg
    29761 ctttgtagac gatggcaata cagtcctcgt catcgagcac aatctggatg ttatcaagac
    29821 ggcagaccat atcattgact tgggacctga gggtggtgtc ggtggtggaa ccatcatcgt
    29881 aacaggaact ccagaagaag tagctgccaa cgaagccagc tatacaggac attatttgaa
    29941 aggaaagtta catcatgaat aaacgcgtac aagcatttct agctaaaatg caagaaaaag
    30001 aactagatgg tatcatcatc aacaatctta aaaacgtcta ttatttgact ggtttttggg
    30061 gctcaaacgg aacagtcttt atcagccgtg accgtcaggt cttagtgaca gactctcgct
    30121 atatcatcgc agctaagcaa gaaaccagtg gttttgagat tgtggctgat cgtgatgaat
    30181 tggctgtcat tgcaggaatt gttaaggaca tggacttgac tcgtatcggt tttgaagatg
    30241 agatttcagt gtcttattac caccgtatgc aggcagcttt tgcaggtttg gacttgcttc
    30301 cacaaactca gtttgtggaa ggtcttcgta tgattaagga tgaggcagag attgcagcga
    30361 ttcgcaaggc ttgttctatc tcagaccaag ctttccgcga tgcgcttgac tttatcaaac
    30421 caggaaaaac tgaaattgag attgccaact tccttgattt ccgcatgcgt gagttgggag
    30481 catctggctt atcttttgat acgatcctag ctagcggtat caattcttct aaaccccatg
    30541 cccatccaat gcacaaacca gtggagttgg gagaagccat taccatggac ttcggctgtc
    30601 tctatgacca ctatgtcagt gatatgaccc ggactatcta tctagggcat gttagcgatg
    30661 agcaggcaga gatttacaat acggttctaa aagctaacca agccttgatt gaccaagcta
    30721 aggcaggctt aggtttccgt gactttgaca aaatccctcg tgatattatc attgaggcag
    30781 gttatggcga ctactttact cacggcattg gccacggtat tggtctggat atccatgagg
    30841 aaccctactt tagccagact tctacagaaa ctattaagac aggtatggcc ttgaccgatg
    30901 aaccaggtat ctatatcgaa ggcaaatatg gcgttcgtat cgaggatgat atcctgatta
    30961 cagagacagg ttgtgaatta ttgaccctag ttccaaaaga gttgatagtc atttagttgt
    31021 ttgtcaagaa aaaagtacga aaatgacgaa aaaagtcaaa aaaaattaaa aataggtcgc
    31081 aagtcggatg ttttttatgg tataatagac taaactgata gtaacatgta gcgaaagggg
    31141 taggtacatg attaaaattt atacagtctc aagttgtact agctgtaaaa aagcaaaaac
    31201 ctggctcaat gcccaccagt taagttataa agaacaaaac cttggtaaag aaggaattac
    31261 gagagaagaa ttactggata ttctaaccaa aacagataac ggaatagcca gcatcgtttc
    31321 atctaaaaat cgctatgcca aagcccttgg agtggatatt gaagatttga gtgtcaatga
    31381 agttctcaat ctgattatgg aaacaccgag aattttaaag agcccaatcc ttgtagatga
    31441 aaaacgcctg caagttggct acaaggaaga cgatattcgt gccttcctac cacgctctgt
    31501 ccgtaatgta gaaaatgcag aagcacgttt gcgtgcagct ctataaacat caaggctggg
    31561 agcacttccc agtcttattc tattttaatt tcaaaaagaa agaagaaaga aatgaaaaaa
    31621 atagttcttg ttagtctagc tttccttttt gtcctggttg gttgcggaca gaaaaaagaa
    31681 actggaccag ctacaaaaac agaaaaagat acgcttcagt cggcattgcc agttattgaa
    31741 aatgctgaga agaatacagt tgtaactaag actttggtct tgcccaagtc agatgatggt
    31801 agccagcaga cacaaacaat tacttacaaa gacaagactt ttttgagtct aattatccaa
    31861 caaaaacgtc cagtctctga tgagttgaag acttatattg accaacatgg agtggaggaa
    31921 actcaaaaag ctcttcttga agcggaggag aaggataagt ctatcattga agctcgtaaa
    31981 ctggcaggtt tcaaacttga aacaaaacta ttgagcgcaa cggaacttca aacaacgact
    32041 agttttgatt ttcaagttct ggatgtcaag aaggcttccc agttggaaca tctgaagaat
    32101 attggtttgg aaaatctttt gaaaaatgaa ccaagcaaat atatttcaga tagattggca
    32161 aatggcgcga cagaacaata gaaaatccaa ataaatagac tgcctgaatt gtagaaggta
    32221 aggaattatg ctataatggt actattctaa ggaaagaggg tgttagaatg ggatttactg
    32281 aagaaacagt acgttttaaa ttggacgatt ccaataaaaa agaaattagc gaaactttga
    32341 cagatgttta tgcttcgttg aacgataagg gttacaaccc aattaaccaa atcgtaggtt
    32401 acgtattgag tggagaccct gcctacgttc ctcgttataa taatgcacga aatcaaatcc
    32461 gtaagtatga gcgtgatgaa atcgttgagg aattggttcg ctactacctc aaaggacaag
    32521 gagtcgatct ataacctatg agaattatgg gattggacgt cggttcaaaa acggtagggg
    32581 tggcgattag cgatccgctt ggttttacag ctcaagggct tgaaatcatc cagataaatg
    32641 aagaacaagg caaatttggt tttgaccgcg ttaaggaatt ggttgatact tacaaggtgg
    32701 aacgatttgt agtgggcttg cctaaaaaca tgaacaatac aagtggaccg cgcgtagaag
    32761 ctagtcaagc ctacggagca aagctagaag agttttttgg tttaccagta gactatcagg
    32821 atgaacgctt gacaacagtg gctgctgagc gcatgttgat tgaacaagca gatatcagtc
    32881 gcaataagcg caagaaagtc attgataagt tagcagctca gctgatttta caaaattatt
    32941 tagatagaaa attttaatat aaaggagagg ctatgtcaca cgatcataac cacgaccacg
    33001 aagaacgtga actaatcaca ctagtagatg agcaaggaaa tgaaaccttg tttgaaatcc
    33061 ttttgacaat tgatggaaaa gaagaatttg gtaaaaacta tgttcttcta gtaccagtta
    33121 acgcagaaga agacgaagac ggacaagttg aaatccaagc ttactcattc attgaaaatg
    33181 aagatggaac agaaggcgaa ttgcaaccaa tcccagaaga ctcagaagac gaatggaaca
    33241 tgattgaaga agtcttcaac agctttatgg aggagtgata cagtctggga gactgtatca
    33301 gcccaactcc cagaaatggg gaagagttgg aacgtccagt ggatgttttt agcccagttc
    33361 ctttaaataa gagagaactg gtaggttcgg ggggacgttt ttagcccagc tcctttaaat
    33421 aagatagagc tggtaagtct gggagactgt atcagcccaa ctcccagaaa tggggaagag
    33481 ttggaacgtc cagtgaacat ttttacccca actcctagaa attgagaaga gttggaacat
    33541 tcagtggatg tttttagccc acgttcacat tcatagtagc tagttattag ctaaccaggt
    33601 aagaggtcgg gacgaaaatc ctggccttgt ttttattagt aactatactt taatgcgata
    33661 attgattgga cttttgtcaa ggagatgtgt atgtttgaag tagaagaatg gcttcatagt
    33721 cggattggtt tgaattttcg atcaggtttg ggccgaatac agcaagcggt ggatttgtta
    33781 ggaaatcctg agcagtctta ccctattatc cacgtaacag ggactaatgg gaaaggatcc
    33841 accattgctt ttatgaggga attatttatg gggcatggca aaaaagttgc gacctttacc
    33901 tcccctcata tcgtatccat caatgaccga atttgcatta atgggcaacc tatagcagac
    33961 gcagacttta tccgtttggc tgatcaggtc aaggagatgg agaaaacgct tctgcaaact
    34021 cctgaccagt tgtccttttt tgaattgctg accttggttg cttttcttta ttttagggag
    34081 caggaggtgg atttggtttt attagaagtg ggaattggtg gcttacttga cacgaccaat
    34141 gtggtaactg gagagcttgc tgtcatcacc tccattgggc ttgaccatca agaaaccttg
    34201 ggtgatagtc tagaagcaat tgcagagcag aaagctggta ttttcaaggc tggtaaaaag
    34261 gcagtgattg caaaattgcc tccagaagct aggcttatct gtcagaaaaa agccgaatct
    34321 ttagctgttg acctttatca ggcaggtcaa gatttttcag tgctgaatgg tgatttttca
    34381 agctctttac taaatctttc acagctgaac ataggcttag aaggagccta tcagcaggag
    34441 aatgcggcct tggcgttgca aacttttctt ctttttatgg gagaaagaaa ggaagctatt
    34501 gatgaacagg ctgtaagaaa ggccttggaa cagacccatt gggctggtcg cttggagcgt
    34561 attcgcccac agatttactt ggatggtgct cataacctcc ctgccttgac ttgcttggtt
    34621 gagtttatca aagaaaaaga gcaggaaggc tatcgacctc aaatcctctt tggatccttg
    34681 aaacgtaagg attatcaagg gatgttgggt tatctgactg aaaatttacc tcaagtggaa
    34741 ctcaaaatga ccggctttga ctatcaaggg tctttggacg agacagatgt aacaggttac
    34801 catgtaattc cctcttaccg agaatttatc agcagtttcg aagaaagggc agacgctcaa
    34861 gacttgctgt tcgttacagg gtctctctat tttatctcag aagtacgggg ctacctgctg
    34921 gaccgtgagc agataaattg accccctttt ttgaacttgt tatactaggg gaattgctgg
    34981 ttagagaaaa tttctctaaa ttggtagcag aaaggaaatt catcatgaaa ttaaaaagat
    35041 tcacactttc tcttgcttct ctagcaagtt ttagtctctt agtagcttgt tcacaaagag
    35101 ctcagcaggt ccaacagcct gctcaacaga ataccaatac tgcaaatgca ggaggtaacc
    35161 aaaatcaagc ggctccagta caaaaccaac ctgttgctca gccgaccgat attgatggga
    35221 cttatactgg tcaggatgac ggagaccgta tcactttagt ggtaactgga acgactggta
    35281 catggactga gctcgaatct gacggggatc agaaagtcaa acaggttaca tttgattcag
    35341 caaatcaacg catgattatt ggcgatgatg tcaaaattta cactgtaaac ggtaatcaaa
    35401 tcgtcgtaga tgatatggat agagacccat cggaccaaat cgttttaact aaataagact
    35461 atgcaagtag aaagggctgg atttttcagc ctttttactt ttacctagaa aataatcata
    35521 aatacttgcc ttctaccgaa tacctgcgtt atactagtat caattacttg tttttagcat
    35581 tcaaactatc aaggagggga tatgaaatat aggaaatttc aattattgat gtccaagtat
    35641 ggctttagtc tttcgattat gctacttgaa ctttgtcttg tttttggtct ctttctttat
    35701 ttaggacgca tggctcccat tttatggatt actgtcctca ttctactgag tatcatcaca
    35761 atcatttcga tagtcaaccg taatacgact cctgagaata aggtaacctg gttgttagta
    35821 gcctttgtgc cagtatttgg tcccttgctc tatctgatgt ttggtgaaag gcgattgtcc
    35881 aaaaaagaaa tcaaacaact gaagaagcta ggctctatgc atttccaaga agcaaatagc
    35941 cagctactaa aagagaaatt aaaagaaagt gacaaggcag cttatggagt catcaagtcc
    36001 ttattgagta tggataccaa tgctgacatc tatgatcaaa ctgcctctac attttttcct
    36061 aacggagaag ctatgtggaa aaagatggta gaagatctta aaaaggctga gaaatttatt
    36121 ttcttggaat attacattat agaagaaggt ttgatgtgga atcgcatact agatatacta
    36181 gagcaaaagg tagctcaggg tgtagaggtt aagatgctat atgatgatat cggctgtatg
    36241 gctactttaa caggagatta tgcacatcga cttcgtcagc tgggcatcga ggcccataaa
    36301 ttcaataaag ttattcctcg tttgacagtg gcttataata acagagatca tagaaaaata
    36361 ttgattgttg atggtcagat agcctatact ggtgggatca atctggcaga tgagtacatt
    36421 aaccacgtcg agagatttgg ttattggaag gatagtggaa ttcgcttaga cggactagca
    36481 gtaaaagctc tgacacgctt atttttgacc acttggtaca ttaatcgagg agaaattagt
    36541 gattttgatc aatatcattt agaaaatcat tctatcccga gtgacggttt aaccattcca
    36601 tacggaagtg gacccaagcc aatttttcga gcgcaggtag ggaaaaaagt ttatcagagt
    36661 ttaatcaatc aagcaacaga atcggtctat attacgacac cttatttgat tatagattat
    36721 gatttaacag agacaatcaa aaatgcagct atgagagggg tcgatgttcg aattatcacc
    36781 ccttacatac cagataagaa gttcattcag ttagtcacga gaggagctta tcccgacctt
    36841 ctttctgctg gtgttcggat ttatgagtat agtccaggtt ttattcatag taagcagatg
    36901 ttggtagacg aagattttgc ggtggtgggg acaatcaatc tcgactaccg gagcttggta
    36961 caccattatg aaaatgcagt cttactctat aaaactcctt ctataaggga aatcgcccga
    37021 gattttcgaa atatatttgc agattctcag gaagtctatc ctcattctat caaaacgagc
    37081 tggtatcaaa agcttgtaaa agaaatcgcc cagctattcg cccctatctt ataagaaatc
    37141 tagaacttag gatacaaacc agacctattt ttcttgtctt ttacgaatag aaagaaagag
    37201 gaggggaatt catgctaact aaggaagaag tgaatgatat gattgagttc aaacagacac
    37261 atttacaccg ctttcgggaa attgaaacct ttgtgaaact atgcaaaaaa tcactcaaac
    37321 agccatcgca agctgacaat cctaggactt tttgatatac taagacagat aaattgaaca
    37381 ggagaaacga tatgagtgtg tatggtagag tagaagaagt tcataaggag aatcgtgaac
    37441 ctctagaata ccaaatcgaa caagaatcgc atcatcgtga atcaagtcgt cttcctttgg
    37501 tgaaaatttt actatggagt acgcttgtaa cggggataac tctaggagta ccgctattac
    37561 tcgacttaat gagtgcacaa gaagtgcagg atttttatgc aggttgggcg cttcatcaga
    37621 cagggaagat ttacagcgac tattatggaa gtcaaggttt gctttattat ttgctgactt
    37681 acgtgagtca gggcggattt ttctttgcca tctttgagtg gttagccttg gtagcaggcg
    37741 gatttttcct ttttagatca gcggacacct tgacagagca aggagaccaa gctggacatc
    37801 tggtgactat tttttacatg ctagttacag gtcttgcttt tggtggaggc tatgcgactc
    37861 ttttagcgct tcctttctta ttcgcagcct ttagtttagt tgcggcttac ctaagcaatc
    37921 caagccatga taagggattt gtacggattg ggctagcttt ggcaggcgga tttttctttg
    37981 ctcccttatc atcgctcctg tttattgctg tagtgagttt aggcttgttg gtctttaacc
    38041 ttgggcatag acgttttgtg catgggtttt atcagtttct tgcagtggct ttaggttttt
    38101 cacttgtctt ttatccaact gcctactata gtgctgcaac aggaagtttt ggggatgcga
    38161 ttagtggtat tcgttatcct attgacagta ttcgctttga ttttacttct aaaattttag
    38221 agaatatgtt tttttatggc ttgttgtccc ttggtttggg atttgtggtt tgtatctttt
    38281 taggtctctt tcaatccaaa ccctttaaac tatacgtcat ttcggttcct gcaagtcttg
    38341 tggtaatttt aggtttgatt ttacttttct tttcacaaga gcctctgcac gcttcttatt
    38401 tgatggtcgt cttccctgtt ttcctacttt tattggtaac caatattaag agtcaacaga
    38461 gggggcgtag tgctagaaga agccgaagag aaacgccagt tagcctatgg agtcgtttct
    38521 tcaaaggaaa tctatatctg ctagtttttg ggtttgtcta tcttttgtct gttccttttt
    38581 tgatgaagtt tgtcctttat ccagtacctt atcaagaacg taatcgtctt gctgatttgg
    38641 taaaagagga gacaaatacg gaagatgcta tctatgcatg ggatgatact gcgactcttt
    38701 atcgtaagag tgagcgcttg tcgccatcgg cgattttgtc cccgttgcac tatacagcaa
    38761 ctgaggaaaa tcgtaataag ttacttaatg acttgaaaga aaaacaacct aaggtgattg
    38821 tggtaaatga caaggtggta gtctggtctg aagtggaaac actcttaaaa gaaaattacc
    38881 aacaagtaaa gactgattac tcagagttta aagtctataa aattaaataa ccaaatcaat
    38941 atcttgtgta tttttaaaaa ttttaggatt tttaacacaa gatattgatt tttcttttta
    39001 gagtggtata atacttttta gaaagaacat tttagaaaag agcatgcgta tgattgcact
    39061 agaagaaaaa attacaattt tgccaactct cttcgtcgag aaacgagatg ggagacgtgt
    39121 tgtatttgat gtggacaaga ttgacaaggc tctccgcaag gcggctgaca aggttatgga
    39181 tgtgacaccc ctggttgaaa aacgcctcaa tgatctgact gagcgaatta ttacagaaat
    39241 tcatagtcgc tttccacagg gaattaagat ttacgaaatt caaaatatcg tagaacatga
    39301 actccttgaa gccaaagaat atgcactggc tgaggagtat attacttatc ggacacagag
    39361 ggattttgag cgctcaaaag cgacggatat caactttagt attcataaac ttctcaacaa
    39421 agaccagaca gttgtcaatg aaaatgctaa taaagacagt gatgtcttta acactcagcg
    39481 tgatttgaca gcagggattg ttgggaaatc aatcggactg caaatgcttc ctaagcacgt
    39541 agccaatgcc caccaaaagg gggatatcca ctatcacgat ttggactaca gtccctatac
    39601 ccctatgacc aactgctgtt tgattgattt taagggtatg ttggaaaatg gttttaagat
    39661 tggaaatgca gaggtagaga gtcccaagtc tatccagact gcgacagcac agatttctca
    39721 aatcattgcc aacgttgctt ctagccagta cggtggctgt tcagctgacc gtatcgatga
    39781 aattttggcg ccttatgcag agaagaacta tcaaaaacat ctcaaagatg cagaagagtg
    39841 ggtattgcct gaaaaacggg aagattacgc ttggaagaaa acgcaaaagg acatctacga
    39901 tgccatgcaa tctcttgagt atgaaatcaa tactctcttc acttcaaatg gacaaacacc
    39961 ttttacttcg ctaggttttg gtctgggaac cagtcgtttt gaacgagaaa ttcaaaaagc
    40021 tattttaaac attcgcatca agggtcttgg ttcagaacac cgtacggcta tctttcctaa
    40081 acttattttc actctgaaaa gaggacttaa tttagaggaa ggaactccca actacgacat
    40141 caagcagttg gctcttgagt gtgcaaccaa acggatgtat ccagatgttt tatcttacga
    40201 taagattgtt gacttgacag gatccttcaa ggtgcctatg ggttgccgtt ctttcctcca
    40261 aggatggaag ggtgaaaatg gtgtagaagt caattcaggt cgcatgaatc tgggtgttgt
    40321 gacggttaat ctgcctcgta tcgctcttga gtctgaaggt gatatgaata agttctggga
    40381 aatcttcaac gagcgaatga atatcgcaga agatgctctt gtttaccgtg tcgaacgcac
    40441 taaagaggcg acaccagcaa atgctcctat tctttatcag tatggtgctt ttggccatcg
    40501 tctaggtaaa gaagaaagtg ttgaccagct ctttaagaat cgtcgtgcga ccgtttcgct
    40561 gggctatatc ggcttgtatg aagtagcgac agttttcttt ggtaacagct gggaaagtaa
    40621 tccagatgct aaggaattca cgctagacat cattcacgat atgaaacgcc gtgtagaaga
    40681 gtggtcagac caatatggct accatttctc tatctactca acaccatccg aaagtctgac
    40741 agaccgtttc tgccgactag atatagacaa gtttggctct attcctgata tcacagacaa
    40801 ggaatactac accaactctt tccactacga tgttcgtaaa aatccaacac cgtttgaaaa
    40861 attggacttt gagaaagtct atccagaagc aggtgcgtca ggtggtttca tccattattg
    40921 tgagtatcca gtccttcagc aaaatccaaa ggccttggaa gctgtctggg attatgctta
    40981 tgaccgtgta ggctatctag gcaccaatac tccgattgac cgttgctaca agtgtgactt
    41041 tgaaggggat tttgaaccaa ctgagagagg gtttgcttgt ccaaactgtg gcaatagcga
    41101 ccctaaaaca gtagatgtgg tgaagcgaac ttgtggctac ctaggtaatc ctcaagcaag
    41161 accgatggtc aacgggcgtc acaaggaaat cgctgcgcgt gtcaaacata tgaatggttc
    41221 aacgattaaa atagctgggc atcaagtaac aaattagaaa gaaatgaaat gggaaaatat
    41281 caactagacg ataaggggcg cgcacaagtg acccgttatc acgagaaaca ctctaaaggt
    41341 ggagctggta agaaagaacg cttgcttagc ttcagagaac aatttttaaa caagaacaag
    41401 aaaaaataaa agtgagagcc agctctcgct tttctcatag tgggaggtaa ggatggaatt
    41461 acgcagacca agattagcgg ataagaaagc tgttttagat atgatgacag agtttgaaaa
    41521 atttcagtcg cctcacgatg gcggtttctg ggatacagag aactttgtgt atgaagactg
    41581 gttagaaagc aatcaggaac aggaaatggg gattaatctg cctgaaggat gggttcctgc
    41641 aattcagtta gtggcttttt ctgagaaagg tcaagcagtt ggatttctta atctccggtt
    41701 gcgcctcagt aactttctac tagaagaagg tggccacatt ggctactcca ttcgtccatc
    41761 tgaaagaggc aagggttatg caaaagagac tcttcgtcag ggcttgcaag ttgctaagga
    41821 aaagaacatc aagaaagctc tggtgacctg tagcgtgaat aatcctgcta gcagagcagt
    41881 cattctagca aatggtggaa tatttgagga tgctcgcaat ggagtcgagc gttattggat
    41941 agaggtagcg aatgaataat ccaaaaccac aagaatggaa aagcgaggaa cttagtcaag
    42001 gtcgtatcat tgactacaag gcctttaact ttgtggacgg cgaaggcgtg cgcaactctc
    42061 tctatgtatc aggctgtatg tttcactgcg agggatgtta taatgttgcg acttggtctt
    42121 ttaatgctgg cattccctat acagcagaat tagaagagca gattatggca gaccttgccc
    42181 aaccctatgt tcaaggcttg actttgctgg gaggggagcc ttttctcaat actggcattc
    42241 tcttgcctct agttaaacgc atccgaaagg aattgccaga caaggacatt tggtcctgga
    42301 cgggctacac ttgggaagaa atgatgttgg aaactccaga taaactggaa ctcttatcat
    42361 tgattgacat tcttgtcgat ggaagatatg atcgaactaa gagaaatctc atgctccagt
    42421 ttcgaggttc gtccaaccaa cgaattatcg atgtgcaaaa atcgctcaaa agtgggcaag
    42481 tagtgatttg ggacaagctc aatgacggaa aagaaagcta tgaacaggtg aagagagaat
    42541 gaagaaaaaa gacttagtag accaactagt ctcagagatc gagacgggga aagtcaggac
    42601 actgggaata tacggtcatg gagcttcagg taaatcaacc tttgcacagg aattgtacca
    42661 agctttagat tctactacag taaatttgct agagacagat ccttatatca cctcaggacg
    42721 ccatctggta gtacccaagg acgcgccgaa tcaaaaggtg acagccagtc tgccagtggc
    42781 gcatgaactg gagagtttgc agagagatat ccttgccttg caggcgggta tggatgtctt
    42841 gacaattgaa gaaccttgga aggctagtga ggtcttgtct ggagccaaac caattttgat
    42901 tgtcgaaggg atgtctgttg gctttctacc caaggaactc tttgaaaaaa ccatctgttt
    42961 ctacacggat gaggagaccg aattaaagcg acgccttgct agagatacga ctgtgagaaa
    43021 tcgcgatgca tcctttatat tggctagcca tcagatgaga cgggagcagt atctgcgata
    43081 ctataaagaa actgagtcta aggcggatat cttagtggac caatcagaag ataaatttga
    43141 tgtcaagagg acttaactta tgtatagaag aaatatagaa gaaaatccct aattttagaa
    43201 agaaaaagta ggaaaacagt ttcatcccaa aaaaacgaaa aaacagcaac aaatcccttg
    43261 caatcgcagg ggctttgtgt tattctatta tggtgctgta aattacagcc ttagttttga
    43321 tgcaagaggt tgcgacacgc tcggttgcat tgccacgcaa caccgcgtcg gttttcttgt
    43381 ggagctagcc tattatctta aatagacgaa aaggagaaaa agatggcaaa caaaaaaatc
    43441 cgtatccgtt tgaaagctta cgaacaccgt acgcttgaca cagcggctgc aaaaatcgta
    43501 gaatcagcta ctcgtacagg tgcacaagtt gcgggtccaa tcccacttcc aactgaacgt
    43561 agcctctaca caatcattcg tgcgactcac aaatacaaag actctcgcga acaatttgaa
    43621 atgcgtacac acaaacgttt gatcgatatc gttaacccaa ctcaaaaaac agttgatgcc
    43681 ttgatgaaat tggatcttcc aagtggtgta aacgtagaaa tcaaacttta atctaaaata
    43741 taaaagagca gaggctggtg tttcaatcta attgaacacg ggctaaactc ggtgtgaaaa
    43801 agataaactt cctagtgtct gctagacact gcgtcagttt cctattttca cgttgagttt
    43861 gacgcccttt gtatcttaga cttgagcata aaaaacgctc gttaaaaact ttttgaataa
    43921 aaaatataga aaaggaacta ttttctcatg acaaaaggaa tcttagggaa aaaagtggga
    43981 atgactcaaa tcttcactga agctggcgaa ttgatccctg taacagttat tgaagcaact
    44041 ccaaacgttg ttcttcaagt taaaactgtt gaaacagacg gatacaacgc tatccaagtt
    44101 ggtttcgatg acaaacgcga agtattgagc aacaaacctg ctaaaggaca tgtagcgaaa
    44161 gctaacacgg ctcctaagcg cttcattcgt gaattcaaaa acgttgaagg cttggaagtt
    44221 ggtgctgaaa ttacagttga aacattcgca gctggagacg ttgttgacgt aacgggtact
    44281 tctaaaggta aaggtttcca aggtgttatc aaacgccacg gacaatcacg tggaccaatg
    44341 gctcacggtt ctcgttacca ccgtcgtcca ggttctatgg ggcctgttgc acctaaccgc
    44401 gtattcaaag gtaaaaacct tgcaggacgt atgggtggcg accgcgtaac aattcaaaac
    44461 cttgaagttg tacaagttgt tccagaaaag aacgttatcc ttatcaaagg taacgtacca
    44521 ggtgctaaga aatctcttat cactatcaaa tcagcagtta aagctggtaa ataataaaga
    44581 aaggggaaat cagtcacaat ggcaaacgta acattatttg accaaactgg taaagaagct
    44641 ggccaagttg ttcttagcga tgcagtattt ggtatcgaac caaatgaatc agttgtgttt
    44701 gatgtaatca tcagccaacg cgcaagcctt cgtcaaggaa cacacgctgt taaaaaccgc
    44761 tctgcagtat caggtggtgg acgcaaacca tggcgtcaaa aaggaactgg acgtgctcgt
    44821 caaggttcta tccgctcacc acaatggcgt ggtggtggtg ttgtcttcgg accaactcca
    44881 cgttcatacg gctacaaact tccacaaaaa gttcgtcgcc tagctcttaa atcagtttac
    44941 tctgaaaaag ttgctgaaaa caaattcgta gctgtagacg ctctttcatt tacagctcca
    45001 aaaactgctg aatttgcaaa agttcttgca gcattgagca tcgattctaa agttcttgtt
    45061 atccttgaag aaggaaatga attcgcagct ctttcagctc gtaaccttcc aaacgtgaaa
    45121 gttgcaactg ctacaactgc aagtgttctt gacatcgcaa atagcgacaa acttcttgtc
    45181 acacaagcag ctatctctaa aatcgaggag gttcttgcat aatgaatttg tatgatgtta
    45241 tcaaaaaacc tgtcatcact gaaagctcaa tggctcaact tgaagcagga aaatatgtat
    45301 ttgaagttga cactcgtgca cacaaacttt tgatcaagca agctgttgaa gctgctttcg
    45361 aaggtgttaa agttgccaat gttaacacaa tcaacgtaaa accaaaagct aaacgtgttg
    45421 gacgttacac tggttttact aacaaaacta aaaaagctat catcacactt acagctgatt
    45481 ctaaagcaat cgagttgttt gctgctgaag ctgaataatc taaggaggaa atatcgtggg
    45541 aattcgtgtt tataaaccaa caacaaacgg tcgccgtaat atgacttctt tggatttcgc
    45601 tgaaatcaca acaagcactc ctgaaaaatc attgcttgtt gcattgaaga gcaaggctgg
    45661 tcgtaacaac aacggtcgta tcacagttcg tcaccaaggt ggtggacaca aacgtttcta
    45721 ccgtttggtt gacttcaaac gtaataaaga caacgttgaa gcagttgtta aaacaatcga
    45781 gtacgatcca aaccgttctg caaacatcgc tcttgtacac tacactgacg gtgtgaaagc
    45841 atacatcatc gctccaaaag gtcttgaagt aggtcaacgt atcgtttcag gtccagaagc
    45901 agatatcaaa gtcggaaacg ctcttccact tgctaacatc ccagttggta ctttgattca
    45961 caacatcgag ttgaaaccag gtcgtggtgg tgaattggta cgtgctgctg gtgcatctgc
    46021 tcaagtattg ggttctgaag gtaaatacgt tcttgttcgt cttcaatcag gtgaagttcg
    46081 tatgattctt ggaacttgcc gtgctacagt tggtgttgtc ggaaacgaac aacatggact
    46141 tgtaaacctt ggtaaagcag gacgtagccg ttggaaaggt atccgcccaa cagttcgtgg
    46201 ttctgtaatg aaccctaacg atcacccaca cggtggtggt gaaggtaaag caccagttgg
    46261 tcgtaaagca ccatctactc catggggcaa acctgctctt ggtcttaaaa ctcgtaacaa
    46321 gaaagcgaaa tctgacaaac ttatcgttcg tcgtcgcaac gagaaataat attaaactag
    46381 tcgcttaagc aactagtaaa tccgccagct cggtagcgct ccataggagt gcaagccgct
    46441 gtggtacaac atttaaagga gaaaatataa aaatgggacg cagtcttaaa aaaggacctt
    46501 tcgtcgatga gcatttgatg aaaaaagttg aagctcaagc taacgacgaa aagaaaaaag
    46561 ttattaaaac ttggtcacgt cgatcaacga tcttcccaag tttcattggt tacactattg
    46621 cagtttatga cggacgtaaa cacgtacctg tttacatcca agaagacatg gtaggccaca
    46681 aacttggtga atttgcacca actcgtactt acaaaggtca cgctgcagac gacaagaaaa
    46741 cacgtagaaa ataaggagaa cataaatggc agaaattact tcagctaaag caatggctcg
    46801 tacagtacgt gtttcacctc gtaaatcacg tcttgttctt gataacatcc gtggtaaaag
    46861 cgtagccgat gcaatcgcaa tcttgacatt cactccaaac aaagctgctg aaatcatctt
    46921 gaaagttttg aactcagctg tagctaacgc tgaaaacaac tttggtttgg ataaagctaa
    46981 cttggtagta tctgaagcat tcgcaaacga aggaccaact atgaaacgtt tccgtccacg
    47041 tgcgaaaggt tcagcttcac caatcaacaa acgtacagct cacatcactg tagctgttgc
    47101 agaaaaataa ggaggtaaaa tcgtgggtca aaaagtacat ccaattggta tgcgtgtcgg
    47161 catcatccgt gattgggatg ccaaatggta tgctgaaaaa gaatacgcgg attaccttca
    47221 tgaagatctt gcaatccgta aattcgttca aaaagaactt gctgacgcag cagtttcaac
    47281 tattgaaatc gaacgcgcag taaacaaagt taacgtttca cttcacactg ctaaaccagg
    47341 tatggttatc ggtaaaggtg gtgctaacgt tgatgcactc cgtgcaaaac ttaacaaatt
    47401 gactggaaaa caagtacaca tcaacatcat cgaaatcaaa caacctgatt tggatgctca
    47461 ccttgtaggt gaaggaattg ctcgtcaatt ggagcaacgt gttgctttcc gtcgtgcaca
    47521 aaaacaagca atccaacgtg caatgcgtgc tggagctaaa ggaatcaaaa ctcaagtatc
    47581 aggtcgtttg aacggtgcag atatcgcccg tgctgaagga tactctgaag gaactgttcc
    47641 gcttcacaca cttcgtgcag atatcgatta cgcttgggaa gaagcagata ctacatacgg
    47701 taaacttggt gttaaagtat ggatctaccg tggtgaagtt cttccagctc gtaaaaacac
    47761 taaaggaggt aaataaccaa tgttagtacc taaacgtgtt aaacaccgtc gtgagttccg
    47821 tggaaaaatg cgcggtgaag caaaaggtgg aaaagaagta gcattcggtg aatacggtct
    47881 tcaagctaca actagccact ggatcactaa ccgccaaatc gaagctgctc gtatcgccat
    47941 gactcgttac atgaaacgtg gtggtaaagt ttggattaaa atcttcccac acaaatcata
    48001 cactgctaaa gctatcggtg tgcgtatggg atctggtaaa ggggcacctg aaggttgggt
    48061 agcaccagtt aaacgtggta aagtgatgtt cgaaatcgct ggtgtatctg aagagattgc
    48121 acgtgaagcg cttcgacttg ctagccacaa attgccagtt aaatgtaaat tcgtaaaacg
    48181 tgaagcagaa taaggagaag gcatgaaact taatgaagta aaagaatttg ttaaagaact
    48241 tcgtggtctt tctcaagaag aactcgcgaa gcgcgaaaac gaattgaaaa aagaattgtt
    48301 tgaacttcgt ttccaagctg ctactggtca attggaacaa acagctcgct tgaaagaagt
    48361 taaaaaacaa atcgctcgta tcaaaacagt tcaatctgaa gcgaaataat agactaggga
    48421 aggagaaatt tcaatggaac gcaataatcg taaagttctt gttggacgtg ttgtatctga
    48481 caaaatggac aagacaatca cagttgtagt tgaaacaaaa cgtaaccacc cagtctatgg
    48541 taaacgtatt aactactcta aaaaatacaa agctcatgat gaaaacaatg ttgccaaaga
    48601 aggcgatatc gtacgtatca tggaaactcg cccgctttca gctacaaaac gtttccgtct
    48661 tgtagaagtt gttgaagaag cggtcatcat ctaatcaaac ctgaaaggag aaaactgaaa
    48721 tgattcaaac agaaactcgt ttgaaagtcg cagacaacag cggtgctcgc gaaatcttga
    48781 ctatcaaagt tcttggtggt tcaggacgta aatttgcaaa catcggtgat gttatcgtgg
    48841 catctgtaaa acaagctact cctggtggtg cggttaaaaa aggtgacgtt gttaaagcag
    48901 ttatcgttcg tactaaatca ggtgctcgtc gtgctgatgg ttcatacatc aaatttgacg
    48961 aaaacgcagc agttatcatc cgtgaagaca aaactcctcg cggaacacgt atctttggcc
    49021 cagttgcacg tgaattgcgt gaaggtggct tcatgaagat cgtgtcactt gctccagaag
    49081 tactttaatt tttaggaaca aactagtccc ctagcttcaa gctagggtgc ccttatgggc
    49141 gtaagaaaaa tcaaggagaa acctaatgtt tgtaaaaaaa ggcgacaaag ttcgcgtaat
    49201 cgctggtaaa gataagggaa cagaagctgt tgtccttact gcccttccaa aagtaaacaa
    49261 agttatcgtt gaaggtgtta acattgttaa gaaacaccaa cgtccaacta acgagcttcc
    49321 tcaaggtggt atcatcgaga aagaagcagc tatccacgta tcaaacgttc aagttttgga
    49381 caaaaatggt gtagctggtc gtgttggtta caaatttgta gacggtaaaa aagttcgcta
    49441 caacaaaaaa tcaggcgaag tgcttgatta atcacgaagg aaaggagaag tataatggca
    49501 aatcgtttaa aagaaaaata tcttaatgaa gtagttcctg ctttgacaga acaattcaac
    49561 tactcatcag tgatggctgt gcctaaagta gataagattg ttttgaacat gggtgttggt
    49621 gaagctgtat caaacgctaa aagccttgaa aaagctgctg aagaattggc acttatctca
    49681 ggtcaaaaac cacttatcac taaagctaaa aaatcaatcg ccggcttccg tcttcgtgaa
    49741 ggtgttgcga tcggtgcaaa agttaccctt cgtggtgaac gtatgtacga attcttggat
    49801 aaattggtat cagtttcact tccacgtgta cgtgactttc acggtgttcc aacaaaatca
    49861 tttgatggac gcgggaacta cacacttggt gtgaaagaac aattaatctt cccagaaatc
    49921 aacttcgatg acgttgacaa aactcgtggt cttgacatcg ttatcgtaac aactgctaac
    49981 actgacgaag agtcacgtgc attgcttaca ggccttggaa tgccttttgc aaaataatat
    50041 aggaggtaaa tctaatggct aaaaaatcaa tggtagctag agaggctaaa cgccaaaaaa
    50101 ttgttgaccg ttatgctgaa aaacgtgctg cattaaaggc ggcaggggac tacgaaggtt
    50161 tatctaaatt acctcgcaac gcctcaccga ctcgtttaca taatcgttgt agagttacgg
    50221 ggcgcccaca ttcagtttac cgcaaatttg gtctgagtcg tatcgctttt cgcgaacttg
    50281 cgcataaagg tcaaattcct ggtgtaacaa aagcatcttg gtaatttaag atatcaaggg
    50341 tgtcaaaact ccaagtaaaa ataggaaact tgacgaagaa actaaagttt ctaggaaagt
    50401 ttatcttttt cacacagagt ttagcccggg ttcaattggg cttgccaatt tgaacccgag
    50461 ctacagcttt ggcaaaaaag accaatttgg tttggagcat tgcttctgca ttaaattgtc
    50521 tatttttgct cgtgctgtta cgctctttgt atcatgtatt aactagcaag tgcaacttgc
    50581 aaactactag taagaggaga aaaacaaaat ggttatgact gacccaatcg cagacttcct
    50641 aactcgtatt cgtaatgcta accaagctaa acacgaagta cttgaagtac ctgcatcaaa
    50701 catcaaaaaa gggattgctg aaatccttaa acgcgaaggt tttgtaaaaa acgttgaaat
    50761 catcgaagat gacaaacaag gcgtcatccg tgtatttctt aaatacggac caaatggtga
    50821 aaaagttatc actaacttga aacgtgtttc taaaccagga cttcgtgtct acaaaaaacg
    50881 tgaagacctt ccaaaagttc ttaacggact tggaattgcc atcctttcaa cttctgaagg
    50941 tttgcttact gataaagaag cacgccaaaa gaatgttggt ggtgaggtta tcgcttacgt
    51001 ttggtaaaat caagatacaa agctcgtaaa gaacaaagca aaattaggaa gttggagaag
    51061 tttgtttaca aacaagccaa cttatctatt ttgcacagtt cttagagcgt gttcagttca
    51121 gctcttgaac taaataagta tctgaacccc gtgaaaactg gccgttctgg cctgacaatt
    51181 taacaggaga aaataaacat gtcacgtatt ggtaataaag ttatcgtgtt gcctgctggt
    51241 gttgaactcg ctaacaatga caacgttgta actgtaaaag gacctaaagg agaacttact
    51301 cgtgagttct caaaagatat tgaaatccgt gtggaaggta ctgaaataac tcttcaccgt
    51361 ccaaacgatt caaaagaaat gaaaactatc cacggaacta ctcgtgccct tttgaacaac
    51421 atggttgttg gtgtatcaga aggattcaag aaagaacttg aaatgcgtgg ggttggttac
    51481 cgtgcacagc ttcaaggatc taaacttgtt ttggctgttg gtaaatctca tccagacgaa
    51541 gttgaagctc cagaaggaat tacttttgaa cttccaaacc caacaacaat cgttgttagc
    51601 ggaatttcaa aagaagtagt tggtcaaaca gctgcttacg tacgtagcct tcgttcacca
    51661 gaaccatata aaggtaaagg tatccgttac gttggtgaat tcgttcgccg taaagaaggt
    51721 aaaacaggta aataatgttg agtggttgat catcaaccac caacctattt tccaactttg
    51781 tgcatagcac acgatttaaa actaaagagg tgaaaactgt gatttcaaaa ccagataaaa
    51841 acaaactccg ccaaaaacgc caccgtcgcg ttcgcggaaa actctctgga actgctgatc
    51901 gcccacgttt gaacgtattc cgttctaata caggcatcta cgctcaagtg attgatgacg
    51961 tagcgggtgt aacgctcgca agtgcttcaa ctcttgataa agaagtttca aaaggaacta
    52021 aaactgaaca agccgttgct gtcggtaaac tcgttgcaga acgtgcaaac gctaaaggta
    52081 tttcagaagt ggtgttcgac cgcggtggat atctatatca cggacgtgtg aaagctttgg
    52141 ctgatgcagc tcgtgaaaac ggattgaaat tctaatagga ggacactaga aaatggcatt
    52201 taaagacaat gcagttgaat tagaagaacg cgtagttgct gtcaaccgtg ttacaaaagt
    52261 tgttaaaggt ggacgtcgtc ttcgtttcgc agctcttgtt gttgttggtg accacaatgg
    52321 tcgcgtagga tttggtactg gtaaagctca agaagttcca gaagcaatcc gtaaagcagt
    52381 agatgatgct aagaaaaact tgatcgaagt tcctatggtt ggaacaacaa tcccacacga
    52441 agttctttca gaattcggtg gagctaaagt attgttgaaa cctgctgtag aaggttctgg
    52501 agttgccgct ggtggtgcag ttcgtgccgt tgtggaattg gcaggtgtgg cagatattac
    52561 atctaaatca cttggttcta acactccaat caacattgtt cgtgcaactg ttgaaggttt
    52621 gaaacaattg aaacgcgctg aagaaattgc tgcccttcgt ggtatttcag tttctgattt
    52681 ggcataagaa aggggataaa atggctcaaa ttaaaattac tttgactaag tctccaatcg
    52741 gacgcattcc atcacaacgt aaaactgttg tagcacttgg acttggcaaa ttgaacagct
    52801 ctgttattaa agaagataac gctgctatcc gtggtatgat tacagcagta tctcacttag
    52861 taacagttga agaagtaaac taatgaagtt ttaggggatg tgcactgtac catcccctaa
    52921 aactagatat agtcatctat gatgacatcg tataggcgag ttgatggggg agacaacctt
    52981 ttctccctta tcggcgctag cattttacaa aagaggagaa aataaaaatg aaacttcatg
    53041 aattgaaacc tgcagaaggt tctcgtaaag tacgtaaccg cgttggtcgt ggtacttcat
    53101 caggtaacgg taaaacatct ggtcgtggtc aaaaaggtca aaaagctcgt agcggtggcg
    53161 gagttcgcct tggttttgaa ggtggacaaa ctccattgtt ccgtcgtctt ccaaaacgtg
    53221 gattcactaa catcaacgct aaagaatacg caattgtgaa ccttgaccaa ttgaacgtct
    53281 ttgaagatgg tgctgaagta actccagttg ttcttatcga agcaggaatt gttaaagctg
    53341 aaaagtcagg tattaaaatt cttggtaacg gtgagttgac taagaaattg actgtgaaag
    53401 cagctaaatt ctctaaatca gctgaagaag ctatcactgc taaaggtggt tcagtagaag
    53461 tcatctaaga gaggtgacct atgtttttta aattattaag agaagctctt aaagtcaagc
    53521 aggttcgatc aaaaatttta tttacaattt ttatcgtttt ggtctttcgt atcggaacta
    53581 gcattacagt tcctggtgtg aatgccaata gcttgaatgc tttaagtgga ttatccttct
    53641 taaacatgtt gagcttggtg tcggggaatg ccctaaaaaa cttttcgatt tttgccctag
    53701 gagttagtcc ctatatcacc gcttctattg ttgtccaact cttgcaaatg gatattttac
    53761 tcaagtttgt agagtggggt aaacaagggg aagtaggtcg aagaaaattg aatcaagcta
    53821 ctcgttatat tgctctagtt ctcgcttttg tgcaatctat cgggattaca gctggtttta
    53881 ataccttggc tggagctcaa ttgattaaaa ctgctttaac tccacaagtt tttctgacga
    53941 ttggtatcat cttaacagct ggtagtatga ttgtcacttg gttgggtgag caaattacag
    54001 ataagggata cggaaacggt gtttccatga ttatctttgc cgggattgtt tcctcaattc
    54061 cagagatgat tcagggcatc tatgtggact actttgtgaa cgtcccaagt agccgtatca
    54121 cttcatctat cattttcgta atcattttga ttattactgt attgttgatt atttacttta
    54181 caacttatgt tcaacaagca gaatacaaaa ttccaatcca atatactaag gttgcacaag
    54241 gtgctccatc tagctcttac cttccgttaa aggtaaaccc tgctggagtt atccctgtta
    54301 tctttgccag ttcgattact gcagcgcctg cggctattct tcagtttttg agtgccacag
    54361 gtcatgattg ggcttgggta agggtagcac aagagatgtt ggcaactact tctccaactg
    54421 gtattgccat gtatgctttg ttgattattc tctttacatt cttctatacg tttgtacaga
    54481 ttaatcctga aaaagcagca gagagcctac aaaagagtgg tgcctatatc catggagttc
    54541 gtcctggtaa aggtacagaa gaatatatgt ctaaacttct tcgtcgtctt gcaactgttg
    54601 gttccctctt ccttggtgtg atttccattt taccgattgc agctaaagat gtatttggtc
    54661 tttctgatgt tgttgccttt ggtggaacaa gtctcttgat cattatctct acaggtatcg
    54721 aaggaatcaa gcaattggaa ggttacctat tgaaacgtaa gtatgttggt ttcatggaca
    54781 gaacagaata aaagtattta ctgaatcagt aaatactgag ggagtggagg tttaaactct
    54841 gacatttgta agagttggat ctcccctctt ctattttgtt tttaaatagg ggtgaaaaga
    54901 ctttttgctt ctatttaaaa ataaaataag gagatcaaat catgaatctt ttgattatgg
    54961 gcttacctgg tgcaggtaag ggaactcaag cagcaaaaat cgtagaacaa ttccatgttg
    55021 cacatatctc aacaggtgat atgttccgcg ctgcaatggc aaatcaaact gaaatgggtg
    55081 ttcttgctaa gtcatatatt gacaagggtg aattggttcc tgacgaagtt acaaatggaa
    55141 tcgtaaaaga acgcctttca caagatgata ttaaagaaac aggattctta ttggatggtt
    55201 acccacgtac aattgaacaa gctcatgcct tggacaaaac attggctgaa cttggcattg
    55261 aactagaagg tattatcaat attgaagtga accctgacag cctcttggaa cgtttgagtg
    55321 gccgtatcat ccaccgcgta actggagaaa ctttccacaa ggtctttaac ccaccagttg
    55381 actataaaga agaagattac taccaacgtg aagatgataa gcctgagaca gtaaaacgtc
    55441 gtttggatgt taatattgct caaggagaac caatcattgc tcactaccgt gccaaaggtt
    55501 tggttcatga catcgaaggt aatcaagata tcaatgatgt cttctcagat attgaaaaag
    55561 tattgacaaa tttgaaataa agcgtttttc gcacttgcaa aaatccgcta caaatgttat
    55621 actgagatag tctgacttat aattgttgtc tctgtgtcta gaggcatcga atcgaaattt
    55681 atggaggtgc ttttgcgtgg caaaagacga tgtgattgaa gttgaaggca aagtagttga
    55741 tacaatgccg aatgcaatgt ttacggttga acttgaaaat ggacatcaga ttttagcaac
    55801 agtttctggt aaaattcgta aaaactatat tcgtatttta gcgggagatc gtgttactgt
    55861 cgaaatgagt ccatatgact tgacacgtgg acgtatcact taccgcttta aataatcgaa
    55921 aaacttggag ggataagaaa tgaaagtaag accatcggtc aaaccaattt gcgaatactg
    55981 taaagttatt cgtcgtaatg gtcgtgttat ggtaatttgc ccagcaaatc caaaacacaa
    56041 acaacgtcaa ggataagata gaaaggagaa aacatggctc gtattgctgg agttgatatt
    56101 ccaaatgaca aacgcgtagt aatctcattg acttatgttt atggtatcgg acttgcaaca
    56161 tctaagaaaa ttttggctgc tgctggaatc tcagaagatg ttcgtgtacg tgatcttaca
    56221 tcagatcaag aagatgctat ccgtcgtgaa gtggatgcaa tcaaagttga aggtgacctt
    56281 cgtcgtgaag taaacttgaa catcaaacgt ttgatggaaa tcggttcata ccgtggtatc
    56341 cgtcaccgtc gtggacttcc tgtccgtgga caaaacacta aaaacaacgc ccgcactcgt
    56401 aaaggtaaag ctgttgcgat tgctggtaag aaaaaataat ataggaggta aaagtcttgg
    56461 ctaaaccaac acgtaaacgt cgtgtgaaaa agaatatcga atctggtatt gctcatattc
    56521 acgctacatt taataacact attgttatga ttactgatgt gcatggtaat gcaattgctt
    56581 ggtcatcagc tggtgctctt ggtttcaaag gttctcgtaa atctacacca ttcgctgctc
    56641 aaatggcttc tgaagctgct gctaaatctg cacaagaaca cggtcttaaa tcagttgaag
    56701 ttactgtaaa aggtccaggt tctggtcgtg agtcagctat tcgtgcgctt gctgccgctg
    56761 gtcttgaagt aacagcaatt cgtgatgtga ctccagtgcc acacaatggt gctcgtcctc
    56821 caaaacgtcg ccgtgtataa tcatcgcatt acactgcttt tcgtttaaga gggagtaact
    56881 aaatgatcga gtttgaaaaa ccaaatataa caaaaattga tgaaaataaa gattatggca
    56941 agtttgtaat cgaaccactt gaacgtggct acggtacaac tcttggtaac tctcttcgtc
    57001 gtgtacttct agcttctcta ccaggagcag ctgtgacatc tatcaacatt gatggtgtgt
    57061 tacatgagtt tgacacagtt ccaggtgttc gtgaagacgt gatgcaaatc attctgaaca
    57121 ttaaaggaat tgcagtgaaa tcgtacgttg aagacgaaaa aatcatcgaa ctggatgttg
    57181 aaggtcctgc tgaagtaaca gctggtgaca ttttgacaga tagcgatatt gaaattgtaa
    57241 atccagatca ttatctcttt acaatcggtg aaggttcttc tctaaaagcg actatgactg
    57301 ttaacagtgg tcgtggatat gtacctgctg atgaaaataa aaaggataat gcaccagttg
    57361 gaacacttgc tgtagattct atttatacac cagttacaaa agtcaactat caagtggaac
    57421 ctgctcgtgt aggtagcaat gatggtttcg acaaattaac ccttgaaatc ttgacaaatg
    57481 gaacaattat tccagaagat gctttagggc tttcagcacg tattttgaca gaacatcttg
    57541 atttgtttac aaatcttact gagattgcta agtcaactga agtgatgaaa gaagctgata
    57601 ctgaatctga cgaccgtatt ttagatcgta cgattgagga actggacttg tctgtgcgtt
    57661 catacaactg tttaaaacgt gccggtatca atactgtgca tgatttgaca gaaaaatctg
    57721 aagcagagat gatgaaagta cgaaatcttg gacgcaagag tttggaagaa gtgaaactca
    57781 aactcattga tttgggtctt ggattaaaag ataaataaag gaggaataca tggcttaccg
    57841 taaactagga cgcactagct cacaacgtaa agcaatgctt cgcgatttga caactgacct
    57901 tttgatcaac gaatcaatcg tgacaactga agctcgtgct aaagaaatcc gtaaaactgt
    57961 tgaaaaaatg attactctag gtaaacgtgg tgatttgcat gcacgtcgtc aagcagctgc
    58021 tttcgtacgt aatgaaatcg catctgaaaa ctatgatgaa gcaactgata agtacacttc
    58081 tactacagca cttcaaaaat tgttctcaga aatcgcacct cgttatgctg aacgtaacgg
    58141 tggatacact cgtatcctta aaactgaacc acgtcgtggt gatgcagcgc caatggcgat
    58201 catcgaatta gtataaaatc atcaattttg ttgagtgtta tgatgatgga gtcttgtgct
    58261 cttagtctag ctctggtcta ccgctaggat ttcggtccta gcgggaacac tcatcataag
    58321 ttgggatagt agacgcttgt ttacgaaatt gtttttttct taagaacaac ttcgtaagca
    58381 ggcgtttttg agtattttcg ttagaattat gctatactat ttgaaaagaa tcctgtttaa
    58441 tgttaaggtt tcttatttta agaagaattg gagtttactt atgaaagcca ttataactgt
    58501 tgttggtaaa gataaatctg gaattgttgc aggtgtttct ggtaaaattg cagaattagg
    58561 attgaatatt gacgatatct ctcaaactgt cttagatgaa tattttacga tgatggctgt
    58621 tgtatctagt gatgaaaagc aagattttac ctatcttcgt aatgaatttg aagcttttgg
    58681 gcaaactttg aatgtaaaaa tcaatattca gagtgcagcg attttcgaag ctatgtataa
    58741 tatctaggag gtcatcatgg atattagaca agttactgaa accatcgcca tgattgagga
    58801 gcaaaacttc gatattagaa ccattaccat ggggatttct cttttggact gtatcgatcc
    58861 agatatcaat cgtgctgcgg agaaaatcta tcaaaaaatt acgacaaagg cggctaattt
    58921 agtagctgtt ggtgatgaaa ttgcggctga gttgggaatt cctatcgtta ataagcgtgt
    58981 atcggtgaca cctatttctc tgattggggc agcgacagat gcgacggact acgtggttct
    59041 ggcaaaagcg cttgataagg ctgcgaaaga gattggtgtg gactttattg gtggtttttc
    59101 tgccttagta caaaaaggtt atcaaaaggg agatgagatt ctcatcaatt ccattcctcg
    59161 cgctttggct gcgacggata aggtctgctc gtcagtcaat atcggctcaa ccaagtctgg
    59221 tattaatatg acggctgtgg cagatatggg acgaattatc aaggaaacag caaatctttc
    59281 agatatggga gtggccaagt tggttgtatt cgctaatgct gttgaggaca atccatttat
    59341 ggcgggtgcc tttcatggtg ttggggaagc agatgttatc atcaatgtcg gagtttctgg
    59401 tcctggtgtt gtgaaacgtg ctttggaaaa agttcgtgga cagagctttg atgtagtagc
    59461 cgaaacagtt aagaaaactg cctttaaaat cactcgtatc ggtcaattgg ttggtcaaat
    59521 ggccagtgag agactgggtg tggagtttgg tattgtggac ttgagtttgg caccaacccc
    59581 tgcggttgga gactctgtgg cacgtgtcct tgaggaaatg gggctagaaa cagttggcac
    59641 gcatggaacg acggctgcct tggccctctt gaacgaccaa gttaaaaagg gtggagtgat
    59701 ggcctgcaac caagtcggtg gtttatctgg cgcctttatc cctgtttctg aggatgaagg
    59761 aatgattgct gcagtgcaaa atggctctct taatttagaa aaactagaag ctatgacggc
    59821 tatctgttct gttggattgg atatgattgc catcccagaa gatacgcctg ctgaaaccat
    59881 tgcggctatg attgcggatg aagcagcaat cggtgttatc aacatgaaaa caacagctgt
    59941 tcgtatcatt cccaaaggaa aagaaggcga tatgattgag tttggtggtc tattaggaac
    60001 tgcacccgtt atgagggtta atggggcttc gtctgtcgac ttcatctctc gcggtggaca
    60061 aatcccagca ccaattcata gttttaaaaa ttaagaaaat aggagaaatt ttaagttcta
    60121 tttaagatta gatgtgtata ctataatcat taaataaaga cctcctaata ttatttgaaa
    60181 cagataacac tgaattagtt tgaatttgat tttcatctaa tatctttatt taatgaactc
    60241 ctaaactttt tcataataat ctccttcaaa agtcgcctgt atgggtggct tttattttat
    60301 cattcatgat ataatagaag caaacggagg acggaaaatg gtaaaagtac gattgtattt
    60361 ggtacgtcat ggcaagacca tgtttaacac gattggtcgc gcgcaaggtt ggagcgatac
    60421 tcccttaact gctgaaggtg aacgagggat tcaagagtta ggaatcggtt tgcgagaatc
    60481 tgatctacag tttgagcgcg cttattcgag tgattctggt cgtaccattc agaccatggg
    60541 aattatcctt gaagagcttg gcttgcaggg gaaaatccct tatcgcatgg acaagcgtat
    60601 cagagaatgg tgtttcggta gttttgatgg agcctatgat ggcgatcttt tcatgggcat
    60661 tattcctcgt atctttaatg tggaccacgt tcaccaattg tcttatgctg aactggctga
    60721 gggcttggta gaggtcgata cagctggttg ggctgaaggc tgggaaaaac tcagtggccg
    60781 aattaaggaa ggctttgaaa tgattgcaaa agaaatggaa gatcaaggtg gaggtaacgc
    60841 ccttgttgtc agccatggaa tgactattgg aaccattgtt tatctgatta atggcatgca
    60901 tccgcatggt ctagataatg gtagcgtgac gattcttgaa tatgaggacg gtcagtttag
    60961 ggttgaagtt gtcggtgacc gtagttaccg agagctagga cgtgagaaga tggaagaagg
    61021 ctctatttaa tcagtctaga cttgcttgcc atgagctagg gatttgataa gaatatcaag
    61081 ataagaaaaa acagccgagg gtactccttt cggctgtttt tgatgtggaa aactaaagtg
    61141 taatgctatt gcttttagag attttcataa acaagagcaa ggaacctact gttagaacag
    61201 tcaggatagt tgacaaggct gcgcctacac cgtaatttcc tctgagaacc tctgtataaa
    61261 tagctacagt cattgttctt gttttgacat tgtagaggag gatagaggat agaagtagag
    61321 agttctgaaa tcagtgtgac ccaagatagg atggctccag aaataatacc cgatagcatc
    61381 attggagttg taatcttagc aaaggtattg agacgactac ttcctaagct ttcagcagct
    61441 tcttcaatac ttggtgctat ttgttgtaag ctagcaacag atgagcgaat agtatacggt
    61501 aatcttctga cagatagaga cataatcaag atgaaagcag tccctgtaat cataagaaat
    61561 ccacttccaa atagaccagt atttaaggaa gaaatgaagg caatccctag aacggttcct
    61621 ggtacaatat aaggtaccat actgaggctg tcaattaagt ttgtaaacaa attccgtttt
    61681 ctaacggcta ggtaggagat aaatgtcgca aatagaacaa ctagaactaa ggcaatcaaa
    61741 gggatacgaa tggtattgaa aatagcagat cccatacgat tgaaagctag cttgtaactg
    61801 tttggagaat aacctttaaa aaataccata cctgatgttt ttaggaaaga ggtataaatt
    61861 aagtagattt gaggtaaaac agagataaag ataattccgt agactgttgc ataaatggca
    61921 gccatttttc cttttgtagt ttttttaggc tcaattggat ggagcagatt catgctgaaa
    61981 ctgtagcggt ttgcaatgtg tttttggata aggaaaattg ccaaggcaat gataatcgcc
    62041 ataattgcaa aagcagaatt tcctccaacc tcgctaataa attgggtata aatcaggaca
    62101 gggaaagtcc gatatccttc gccaatcaac ataggcgttc caaagtctga gaatgctctc
    62161 ataaatacaa gcaaggagct gctagtaagg ttggaactag aagaggtaaa acaaccgtta
    62221 cgataggttt aaatccgaag gaccccatgc tttcagctgc ttcaagtaga gaattgtcaa
    62281 tactgttcat tgttccagca acatatagaa ataccagtgg gaatagttgc agtgtaaaga
    62341 caagtacaat tcctttgaat ccataaatat cgatagctgg aagatgaagg gcatttgtca
    62401 aaaatttagt gatgacccca tttcgtccca gcaagagaac ccaggagtag gctcctacga
    62461 aaggagctga catggaagca atgataatca atatttgtag aaatttcttc cccttgaagt
    62521 catacataga gaagagataa gctaataggg ttcctacaac taaggaagtg acagtagcgg
    62581 taatggaaac cctgaaactg ttgactagtg tctcagagta gtaggcttta ctaaagaaag
    62641 tgacaaaatt agctagtgag aattgtcctt catgtataag tgcttgcttg agcacggtaa
    62701 tgataggata aacgagaaag acaagatagg taagaaagat gaagaaagag gaggctgtcc
    62761 aaatatttag ttttttacgt tccatggttg actcctttta tcaggttttg ggaaccatct
    62821 gcagaaaaga tgtttaattt ttgcgtattg attcgtagac gaatacgatt gcctttttgt
    62881 agttcttctt caaaagttga ttcttcacta acttgaattt ttgaggcaaa acctgtctca
    62941 atgaaatact ccgtatttag tccaagatag acgctatcgc taatagttcc ttcaatatct
    63001 ccagattcat ctttgataaa ctcttcggga cgaatgctta catgaatagc ttgctcctca
    63061 acctgatcaa gagctggcat tcgaagggca tagccatctg aaaagacgat ataagcgccg
    63121 tcgctccgtt tttcaagatt agtagggata atatttgtgc gtccgataaa ggttgccaca
    63181 aactcattag ctggtttatg atagagttct tttggtcggc cgatttgttg gatcacccca
    63241 tctttcataa cagcaatttg atctgaaata gccatggctt cttcttggtc gtgggttaca
    63301 taaacagttg taattcccac ttcgtgttgg atttctcgga tggcttgacg catatccaag
    63361 cgaagtttgg cctccagatt actaagtggc tcgtccatga ggagaacact tggattaacc
    63421 gctaaggcgc atgccaaggt gacacgttgt tgttgtccac cactgagttt atcgggcttt
    63481 cgatccgcat attgagcaat ttgcatgagt tcaagatact tgttggtctg ttgaatcaat
    63541 tcttcttttg gaaccttctt ttgcttaaga ccaaaagcaa cgttgtctcg gacagtcaaa
    63601 tgtgggaaaa tagcgtagtt ttggaaaacc atcccgatat tgcgtttgct gggttccata
    63661 ttattgattt ttgtatcatc gaagtaaaat tctccatctt tgatactgtt gaaacctgca
    63721 atcatacgaa gaagggtcgt tttcccacat cctgaaggtc caagaagggt aaagagactt
    63781 ccttttggaa ttgtaatgtt caaattctca ataacaggga catcgtggta gattttttgg
    63841 cgttaataat tttgatctca ctcatagtga acctttttta ctgtttagat tggatatctg
    63901 taaagacttc gttgtatttc ttaacgatat ctgatttatt cttgatgaca taatcataat
    63961 cttcagtgag tgttttgatt ttgtcaattg gtttcatgtt ttcgcttgtt ttagcatttt
    64021 tacgaacagg acggttagta gtggttgtac caagtgtatc ttgtacttct tgagagataa
    64081 taaaatcgat aaatttcttg tcattttcca tatttttagc ttttttaacg atagcagcac
    64141 tagcaggtag gaagacggtt ccttcttttg gatagactac tttaatgtta gctccgtcat
    64201 ttaagagttt aactgctgga tcctcataag agagaccaac agccatttct ccatcaacga
    64261 ctgctttata gacaccagat gaacttgaac cgattttata gtgtattgaa actagaatag
    64321 tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat taatttgtcc
    64381 tgttcttatt tcatttcact atgccatcaa taagtgtgaa aagatctttt acataagacc
    64441 aagccttatc atctttgtaa ccaccttgag cttgtagcat atttgttaat tgagcaaagg
    64501 cgctagaaga gtttgctggg tcagcagttg cgatttttcc ttttagttca ggtttgagaa
    64561 ggtcgctata tccttcgatg ttcatgcctt tagttaaatc agggtggacg attaaaacac
    64621 taccatctag tgtataagga gtagagaagc cagttgtgtt ttgatattct ttgataacat
    64681 tatcattttc ttttgaaata tagttttcaa agagttctcc gtgggtagca tattgtgtat
    64741 aagaaccacc aaagataaca tcagctacag gaacttcttt ttctgactct agttttttga
    64801 aaagttctcc agtaccagct tgaatcagtt ctattttgat accatatttt tcttcaaagg
    64861 caggaatagt tgctccaatt aagccttctg agtttggtga ataaacgact agcgaaccac
    64921 cgtctcctct atcagatgaa ctgtcatcgg cagattcatt agaagaacaa gcagcataat
    64981 acatccattt ctttttcatg atggatacct ccgttgtgtt atttaagttt attttaaaac
    65041 aatgtaagcg tttttaaaac ctacaattct attctatagt gtattgaatc tataacagta
    65101 cactttgact gctaaaatat ttctataaat taatttgact ttcctgatag agatgttcac
    65161 atcttatttc aattcactat attagagtaa aattctctac aaaaagaaga atagcctatt
    65221 ttactattct tccgagtgat ttcaattttt ttggggaaat atggagatac tttttaaata
    65281 ctttggcaaa atatttatag tctgaaaatc caacttggtc agagatatcg cttaaagagg
    65341 agttgggaga ttcagccatc ttatctatag cttgtttcag gcgatattgt aagatatatt
    65401 catttagagt catggggaag tcttctttga taaccttata tagatgcttg ttatctgcaa
    65461 atagctagag catttcaagg aaatgaacta gaaataacaa ttaatatggt ggttcagaaa
    65521 taccttttga taatcaataa gatcttcagg atgaagtgcg ttgactccat ccatttcata
    65581 tttccgattt aataaacgat atttgttttc accaaattga tgaaaaggga gtagttgaac
    65641 ttggtcgata tttaatgagt taaatagagt agcgaatttt tctgcatcct ctaaactatt
    65701 gttaaaatta ggaataactg ggattcttaa aacgatagtt ttattttgag aaaaagcata
    65761 atgaatgttt ttaataatca tttgattaaa aaccccagtc acttttttat gttttataga
    65821 attataatgt tttaggtctg tgtagataaa atccacatat tgaattaaat caataaattt
    65881 ttcatgatca acaaaggcag tagtttcaat ggcagtgtgt atgtgatgtt ctttagctga
    65941 ttttaagatg gctttagcaa attcaaactg agcaaatatt tcacctcctg ataaagttaa
    66001 acctccaccg gattcttcgt aaaattcttt gtcttttaat acctctgtaa taatttcttc
    66061 tacagtcttt tcttcaccga ctaaggtgaa tttctctcgt tgagcatctt tcattttttc
    66121 aggtttcatt ctttgagatt caggattaga acaccatgga cagcgcagag gacatccttt
    66181 taaaaaaaca gttgtacgaa tacccgggcc gtcatgaatt gaaaagtgtt gaatattaaa
    66241 aataattcct ttagatattt ccataagagt tcctcctctt cataagtcta tcctatcaca
    66301 aaaacgaaag aaaaacaatt acgaacgaaa gtttaatttg ttgttttttg ctttttattt
    66361 tggtagaata aaacggaggt gtaaaatgaa aagattggaa caaattatta aattagtatc
    66421 agaacatgaa aagatcgacg ttaatacatt atcggaaaaa ttaaatgtat cgaaagtaac
    66481 gattagaaaa gatttagata aattagagtc aaaaggttta ttacacagag agcatggata
    66541 tgctgtatta aatagtggag atgacttaaa tgtacgtttg tcaattaact atgaaattaa
    66601 gagaaaaatt gttcaggaag cagtaaaatt ggtgtcagat aatgaaacaa taatgataga
    66661 atctggatcg acctgtgctt tacttgctga ggaaatttgc aagcaaaaaa gaaatgttac
    66721 gattgtaaca aattcatttt ttatagcaaa ttttgtgaga gcttatgatt catgtcgtat
    66781 tattgttctt ggtggagagt ttcagaagga ttcacaggtt actgtgggac ctttattaaa
    66841 agaaatgata cagacttttc atgtgcatca agcttttgtt gggacagatg gttacgataa
    66901 agagatgggc tttacaggaa aagatttaat gcgcagtgag gtagttcaat atatttcagc
    66961 agcgtcggat aaagtcattg tactaactga ctcaagtaaa tttgataaaa ggggtacagt
    67021 aagaagattt gctttaagtc aagtctatga agtaataaca gacgaaaaac tttctaaaca
    67081 aaatatagct acattagaaa atgctgggat aatggttaag gtagtttcgt aagaggttaa
    67141 atgtatgaat caagatagga ataaactgct ctctaaaatt gcttatctgt attatattga
    67201 aaacttaaat cagtcacaaa tagcagcaaa attaggaatt tatagaacct ctattagtag
    67261 aatgttaaca gaagcaagga atgcaggaat tgttaaaatt gaaatagaga attttgatac
    67321 caatatgttt aagttggaaa attatgtaaa agaaaaatat ggtctggaaa gtttagaaat
    67381 tattccaaat gaatttgatg ataatccaac aattttatct gaaagaattt ctcaagttgc
    67441 agcaggtgtc cttaggaatc taattgatga taatatgaaa attggctttt cttgggggaa
    67501 aagtttaagt aatttagtag atttaattca cagtaaaagt gtccgaaatg ttcacttcta
    67561 tcctctagca ggtggtccta gtcacataca cgctaaatac catgtgaata cactgattta
    67621 tgaaatgtct agaaaatttc atggagagtg tacatttatg aatgcaacga ttgtgcaaga
    67681 aaataaattg ttagcagatg gtattttgca atcaagatat tttgaaaatt tgaaaaatag
    67741 ttggaaagat ttagatatag ctgtagtcgg aattggtgat tttagcaata aaggaaagca
    67801 tcaatggtta gacatgctta cagaggatga ctttaaagaa ttgaccaaag ttaaaactgt
    67861 aggagaaatt tgttgtcgat tttttgattc aaaaggtaaa gaagtctatg aaaacttaca
    67921 ggaaagaacg atagcaatct ctttggaaga tttaaaaaat attcctcaaa gtttagctgt
    67981 tgcttacggt gatacgaaag tatcttcgat tctttctgtc ttgcgtgcta atttagtaaa
    68041 tcatttgatt acagacaaaa atacaatttt aaaagttttg gaagaagatg gggatttgac
    68101 ttttagagag attctaggtg agtgaaaatg atagactgat tcagtttatc gttttttctt
    68161 tttagtcgat tgcacatttg tgcttatata aacaaaaata gtttatctgt tgtttttgga
    68221 ttgacaactt tattatgtag ttgtattcta tagttacaaa agaaaatttt aaaatttcaa
    68281 atgaaaaagc tttttacata gtgaaatgag gaggaattta tggaaattat tgttccagat
    68341 caaattatca tgggtttaat tttatatgct ggtgatgcga aacaacatat ttataaagcg
    68401 ttagattaca taaaaaatgg tacatgtgaa cggtgtgaag aagaaataca gttagctgat
    68461 gcagccttat tagaagccca taatctacaa acaaaatttt tggcacagga agcgtctggt
    68521 acaaagacag aaattacagc tctctttgtt cattcacaag atcatctcat gactagtatg
    68581 acggagatta atttaatcaa agaaattatt agtttgagaa aagaacttca taaaaaataa
    68641 tactagagta ttatcattgt tattaacata gaggaggaaa acataatggt gaagattggt
    68701 ttgttttgtg cagcaggttt ttctactggt atgcttgtaa ataatatgaa aattgcagcg
    68761 caagctagtg gagttgaggc agaaatagag gcgttttctc agtctaaatt agcggattat
    68821 gcgccaaata tagatgttgc actattgggt ccacaagttg cttatacatt agataaatca
    68881 aaagaaattt gtgataagtg tgatgttccg atagctgtta ttccgatgat ggactatggt
    68941 atgttagatg ggaaaaaagt attagatttg gccctatctt tgattagtgg gtaagaaaag
    69001 gagatttatt atgtcaaaga tggatgttca gaaaatcatt gcaccgatga tgaagtttgt
    69061 gaatatgcgt ggcattatag ctctaaaaga tgggatgtta gcaattttgc cattgacagt
    69121 agttggtagt ttgttcttga ttatgggaca attgccgttc gaaggattaa ataagagcat
    69181 tgctagtgtt tttggagcta attggacaga gccgtttatg caagtatatt caggaacttt
    69241 tgctattatg ggtctaattt cttgtttttc aattgcctat tcttatgcta agaatagcgg
    69301 agtagaggct ttaccagctg gagttctatc tgtatctgca ttctttattt tgctaagatc
    69361 atcttatatc cctaaacaag gtgaggcgat tggggacgct attagtaaag tttggtttgg
    69421 aggccaagga attatcggtg ctatcattat aggtttggta gtaggaagta tttatacctt
    69481 ctttataaag agaaaaattg ttattaagat gccagaacaa gttccacaag ctattgccaa
    69541 acagtttgaa gcaatgattc cagcatttgt aattttctta tcttctatga ttgtatatat
    69601 tttagcgaag tcattgacta atggcggaac attcatagaa atgatttatt ctgctattca
    69661 agttccgttg caaggtttaa ctggatcttt gtatggtgct attggaattg cattctttat
    69721 atcatttttg tggtggtttg gtgttcatgg gcaatcggta gtaaatggag tagtgacagc
    69781 tctgctttta tctaatcttg acgctaataa agctatgtta gcctctgcta atctatcatt
    69841 agaaaatggt gcacatattg ttactcaaca atttttagat tcatttttaa ttctatcagg
    69901 ttcagggatt acgtttggtc ttgtagttgc catgcttttt gcagcaaaat caaaacaata
    69961 ccaagcctta ggaaaagttg cagcttttcc agcaatattt aacgtaaatg agccagttgt
    70021 atttggattt ccgattgtca tgaatccagt tatgtttgta cctttcattc ttgttcctgt
    70081 acttgcagct gtgatagtat atggagctat tgcaacaggt ttcatgcagc cattctcagg
    70141 ggtaacattg ccttggagta caccagctat tctatcagga tttttggtgg gtggatggca
    70201 aggtgttgtg actcagttat tagtattggt gatatctaca gtggtttact ttccattctt
    70261 taagatacaa gatcgtttag cttaccaaaa tgaaatcaaa caatcttaga ggtatttgtg
    70321 tgttactgtt aaactcacac atttgtgcta aaaattagag agttaaaatt tttctagtta
    70381 aaagcttgaa aatttctata aaaatcggta ttatattttc gaaagaaata aaaatatttt
    70441 cgaaagaaag gtgcttacga tggtaaatac agaagtagca agaacaacaa tcaagacaga
    70501 atattttggc agccttactg aaaggatgaa caaatatcga gaagatgttt taaataaaaa
    70561 accttatatt gatgctgaga gagcagttct agcaacacgc gcctatgaac gatacaagga
    70621 acaacctaat gtcctaaaac gtgcatatat gctgaaagaa attttggaaa atatgtctat
    70681 ctatattgaa gaagaatcta tgattgcggg aaatcaagct tcttccaata aagatgctcc
    70741 tatttttccg gaatatacgc tagaatttgt tctcaatgag ttggatcttt ttgaaaagcg
    70801 tgatggagat gttttctata ttacagaaga aacaaaagaa caacttagaa gtattgctcc
    70861 gttttgggaa aataataatt tacgtgctag agctggtgcc ttattacctg aagaagtgtc
    70921 agtttatatg gaaacaggat tcttcggtat ggaaggtaag atgaattctg gagatgctca
    70981 cttagcagtt aactatcaga aacttttgca atttggttta agaggttttg aagagcgggc
    71041 tcgtaaagca aaagtagctc tagatttaac agatccagca agtattgata aatatcattt
    71101 ttacgactct atatttatcg taatcgatgc tattaaagta tatgcaaagc gctttgttgc
    71161 tcttgctaaa agtttagccg aaaatgcaaa tcctaaacgt aagaaagaat tacttgagat
    71221 tgcagatatt tgctctagag tcccatatga accggcaact acttttgcag aagctattca
    71281 atcagtttgg tttattcaat gtattttaca aattgaatct aatggccact ctctttcata
    71341 tggccgtttt gatcaatata tgtatccata tatgaaggct gatttagaaa gtggtaaaga
    71401 aacagaagat agcattgttg aacgtctgac aaatctttgg attaagacaa ttacaattaa
    71461 taaggttcgc agtcaatcac atacattttc ttcagcagga agtcctttat atcaaaatgt
    71521 tacaattggt ggacagactc gagataagaa ggatgctgtt aacccattat cttatttggt
    71581 attaaaatca gttgcacaaa cccatctacc gcaacctaat ctaactgtac gttaccatgc
    71641 aggtttagat gatcgtttca tgaatgagtg tattgaagtg atgaaacttg gttttggtat
    71701 gcctgcattt aataatgatg agattattat tccttctttt attgcaaaag gagtattgga
    71761 agatgatgct tatgattaca gtgccattgg atgtgttgaa acggcagttc cagggaaatg
    71821 gggctatcgt tgcacaggta tgagttatat gaacttccct aaggttctac ttatcacgat
    71881 gaatgatgga attgatccgg cttcgggtaa acggtttgca ccaagctttg gtcattttaa
    71941 ggatatgaag aacttttctg aattagaaaa tgcttgggat aaaacactaa gatatttgac
    72001 acgaatgagt gttattgttg aaaattctat tgatttatca ttggaacgag aagttcctga
    72061 tattctatgt tcagcattga ctgatgattg tattggtcgt ggaaaacacc ttaaagaagg
    72121 tggagcagta tatgattata tatcaggatt gcaagttgga attgcaaatt tgtcggattc
    72181 gttagctgca attaaaaaat tggtgtttga ggaagaacgt ataagcccaa gtcaactttg
    72241 gcatgcactg gaaacagatt atgccggaga agaaggtaag gtcattcaag aaatgttgat
    72301 tcatgatgca cctaagtatg gtaatgatga tgattatgct gacaaattgg ttactgctgc
    72361 ttatgacatt tatgttgatg aaattgctaa atatcctaat acacgttatg gaagagggcc
    72421 tattggagga attcgttatt caggaacatc ttctatatca gccaacgtag ggcagggacg
    72481 tggaacatta gcaactccag atggacgcaa cgcgggtaca ccgttagcag agggttgttc
    72541 accatcacat aatatggatc aacacggccc tacatctgtt ttaaaatctg tttcaaaatt
    72601 accaacagat gaaatcgtag gtggggttct cttaaatcag aaagtaaatc ctcaaacgtt
    72661 agccaaagaa gaagataaat taaaactaat tgctttgtta cgaacattct ttaatcgttt
    72721 acatgggtac catattcaat acaatgttgt ttccagagag acgttgattg acgctcagaa
    72781 acatcctgaa aaacacagag acttaattgt tcgtgttgca ggatactctg cattcttcaa
    72841 tgttctttct aaggcaaccc aagatgacat tataggacgt actgagcata ctttgtaaaa
    72901 taaagaggtt ctttttatgg aatttatgct tgacacatta aatttagatg agattaaaaa
    72961 gtggtctgaa attttgccgc tagctggggt aacttcaaat cccactattg caaaaagaga
    73021 gggttctatt aatttttttg aacgaatcaa agatgtaaga gaattgattg gctctacacc
    73081 ctctattcat gttcaggtga tttctcaaga ttttgaaggc atcttaaagg atgctcatga
    73141 aattcgaaga caagcaggag atgatatatt tatcaaagta cctgttactc cagctggatt
    73201 acgtgcaata aaggtgctaa aaaaagaggg ctaccatatc actgcaacag ctatttatac
    73261 agttattcag ggattattag ctatcgaagc aggagcggat tacctagctc catattataa
    73321 tagaatggaa aatctgaaca ttgattcaaa ttctgtcatt cgtcaattag ttcttgctat
    73381 tgatagacag aactctccta gtaagatttt ggctgcatcc tttaaaaatg tagcacaagt
    73441 aaataatgct ttagctgcag gtgcgcatgc tgttacagca ggagcagatg tttttgaatc
    73501 agctttcgcc atgccatcta tccaaaaggc ggttgatgat ttttctgacg attggtttgt
    73561 tactcaaaat agtcgttcca tttagataga gaggaaatac atatgagaat ttttgctagt
    73621 ccttctagat atattcaggg ggaaaatgcc ttgtttgaaa atgccaaatc aattttggat
    73681 ttgggaaatt accctattct attatgcgat cagttggttt atgatattgt tggaaaacga
    73741 tttgaagatt atctacatag gtatggtttc catattgttc tggcgctatt taatggtgaa
    73801 gcttctgaca atgaaatcaa tcgagttgtt gccttggctg agaaagaaaa ttgtgatagt
    73861 attatcggtc ttggtggggg aaagacgatt gatagcgcaa aagctattgc agatttgatt
    73921 gaaaagcctg ttattattgc tccaacaatt gcatcgaccg acgcacctgt atctgcttta
    73981 tctgttattt atacagatga aggtgcattt gatcattatc tattttattc taaaaatcca
    74041 gatttagttt tggttgatac aaaagttatt tcacaagccc ctaagcgttt attagcgtct
    74101 ggtattgcag atggtttagc aacttgggtt gaggcgcgtg cggttatgca ggcaaatgga
    74161 aaaactatgt tgggacaaca gcaaacattg gctggagttg caattgcgaa gaaatgtgaa
    74221 gaaacgctgt ttgcagatgg tttacaggct atggcagctt gtgaagctaa agtggtgaca
    74281 ccagcattag aaaatattgt tgaagctaat actttattga gtggtctagg ttttgaaagt
    74341 ggaggattag ctgcggcgca tgcaattcat aatggtttta ctgcattgac aggtgacatt
    74401 catcatttaa cacatggtga aaaagtagct tatggaactt tagtacaact attattggaa
    74461 aatagaccta aagaagaact tgataagtat attgagtttt acaaaaaaat tggtatgcca
    74521 acaactctaa aagaaatgca tttggatcaa gttggatatg atgatttaat aaaagttggt
    74581 aaacaagcaa ctatggaggg tgagacaatt catcagatgc cgtttaagat ttcgccttca
    74641 gatgttgctc aagctattat cgctgtagat gcctatgtaa attcaaaata aacaataagg
    74701 actactgttt tccaaatggt agtcttttat tgatccccgt attgaattct atagaagatt
    74761 gaaataggat gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc
    74821 aattgtttcg tactatttca gattcagtct actatacgtt cttcataaat caaaaagcga
    74881 cataggttgt cggctattta ttgtgaatac attaattagc attccagttt tatcttcggt
    74941 ctaaaataag tattttgtgc tatacgagat aagcgtcttg acttactcct tgatttactg
    75001 cataacaatg ggataaaaag tgggagatag agcaattcat agtcatcaaa attaatgaga
    75061 tacagtatac agttttttct ttaaacacat ttcaaattcc ctcaaaaatg gtataatagt
    75121 aacatcacaa aattggagag agaccatgag tttttataat cataaagaaa ttgagcctaa
    75181 gtggcagggc tactgggcag aacatcatac atttaagaca ggaacagatg catcaaaacc
    75241 taagttttat gcgcttgata tgttccctta tccgtctgga gttggtctgc acgtaggaca
    75301 cccagaaggt tatactgcaa ccgatatcct cagtcgttac aaacgtgcgc aaggctacaa
    75361 tgtccttcac ccaatgggtt gggatgcttt tggtttgcct gcagagcaat acgctatgga
    75421 tactggtaat gacccagcag aatttacagc ggaaaacatt gccaacttca aacgtcaaat
    75481 caatgcgctt ggattttctt atgactggga tcgtgaagtc aacacaacag atccaaacta
    75541 ctacaagtgg actcaatgga ttttcaccaa gctttacgaa aaaggcttgg cctatgaagc
    75601 tgaagtgcca gtaaactggg ttgaggaatt gggaactgcc attgccaatg aagaagtgct
    75661 tcctgacgga acttctgagc gtggaggcta tccagttgtc cgcaaaccaa tgcgccaatg
    75721 gatgctcaaa atcacggctt acgcagagcg cttgctcaat gacttagatg aactagattg
    75781 gtcagagtct atcaaggata tgcaacgcaa ctggattggt aaatcaactg gtgccaatgt
    75841 aactttcaaa gtaaaaggaa cagacaagga atttacagtc tttactactc gtccggacac
    75901 acttttcggt gcgactttca ctgtcttggc tcctgaacat gaattagtag acgctatcac
    75961 aagttcagag caagcagaag ctgtagcaga ctataaacac caagccagcc ttaagtctga
    76021 cttggttcgt acagaccttg ctaaggaaaa aactggtgtt tggacaggtg cctatgccat
    76081 caaccctgtc aatggtaagg aaatgccaat ctggattgcg gactatgttc ttgctagtta
    76141 tggaacaggt gcggttatgg ctgtgcctgc ccacgaccaa cgtgactggg aatttgccaa
    76201 acaatttgac cttccaatcg tcgaagtact tgaaggtgga aatgtcgaag aagctgccta
    76261 cacagaggat ggcctgcatg tcaattcaga cttcctagat ggattgaaca aagaagacgc
    76321 tattgccaag attgtggctt gcttggaaga aaaaggctgt ggtcaggaga aggttaccta
    76381 ccgcctccgc gactggctct ttagccgtca acgttactgg ggtgagccaa ttccaatcat
    76441 tcattgggaa gatggaactt caacagctgt tcctgaaact gaattgccgc ttgtcttgcc
    76501 tgtaaccaag gatatccgtc cttcaggtac tggtgaaagt ccactagcta acttgacaga
    76561 ttggcttgaa gtgactcgtg aagatggtgt caaaggtcgt cgtgaaacta acaccatgcc
    76621 acaatgggct ggttcaagct ggtactacct ccgctatatt gacccgcaca atactgagaa
    76681 attggctgat gaggacctcc tcaaacaatg gttgccagta gatatctacg tgggtggtgc
    76741 ggaacatgct gtacttcact tgctttatgc tcgtttctgg cataaattcc tctatgacct
    76801 cggtgttgtt ccgactaagg aaccattcca aaaactcttt aaccaaggga tgattttggg
    76861 aacaagctac cgtgaccacc gtggtgctct tgtggcaacc gacaaggttg aaaaacgtga
    76921 tggttccttc tttcatatag aaacagggga agagttggag caagcgccag ccaagatgtc
    76981 taaatcgctc aagaacgttg ttaacccaga tgatgtggtg gaacaatacg gtgccgatac
    77041 ccttcgtgtt tatgaaatgt ttatgggacc actcgatgct tcgattgctt ggtcagaaga
    77101 aggtttggaa ggaagccgta agttccttga ccgagtttac cgtttgatta caagtaaaga
    77161 aatccttgcg gaaaacaatg gtgctcttga caaggcttac aacgaaacag tcaaagctgt
    77221 tactgagcaa attgagtctc tcaaattcaa cacagctatt gcccaactta tggtctttgt
    77281 caatgctgct aacaaggaag ataagcttta tgttgactat gccaaaggct ttattcaatt
    77341 gattgcacca tttgcacctc acttggcaga agaactctgg caaacagtcg cagaaacagg
    77401 tgagtcaatc tcttatgtag cttggccaac ttgggacgaa agtaaactgg ttgaagacga
    77461 aatcgaaatc gttgtccaaa tcaaaggtaa agtccgtgcc aaactcatgg tcgctaaaga
    77521 cctatcacgt gaagaattgc aagaaattgc tctagcggat gaaaaagtca aagcagaaat
    77581 tgacggtaag gaaatcgtga aagtgattag tgtaccaaat aaattggtta atattgttgt
    77641 aaaataacca atttatttgc tgatttccaa cctcaaacag tttcccaaac tgtttgagcc
    77701 aactaaactc gttaatatcg ttgtgaaata aggtaggaat ccttcagagt agaatctgga
    77761 ggattttttg aatcttctta tgaaagtatg gtatactatg ggcaactata aagtttgaaa
    77821 agtgaaaaaa ggagaaagct atgccagtaa atgaatatgg tcaaatgatt ggggagtcaa
    77881 tggaaggtta tacaccaggt gaactgcctt ctattgattt cttagaaggg cgttatgcta
    77941 ggatagaggc tctttcagtg gaaaagcatg cggaggattt gctagctgtt tatgggcctg
    78001 atacgcctca ggagatgtgg acctaccttt ttcaggagcc agtagcagat atgggggagc
    78061 tggttagcct tttacatcag atgttggctc gtaaggaccg tttttactat gcaatcatag
    78121 acaaggcgac tggtaaggct ttgggatctt tttctctcat gcgcattgac cagaataacc
    78181 gagtaataga agtcggagca gtcacttttt ctccagagct aaggggtaca cggataggaa
    78241 ctgaagctca gtatctccta gctcgctatg tttttgagga gctaaactat cgtcgctacg
    78301 agtggaaatg cgatgcctta aatctgccat ccagacgagc tgcggagcgt ttgagcttta
    78361 tttatgaagg aaccttccgt caggcagtgg tttataaggg acgtacgaga gatacggatt
    78421 ggttgtctat gattgataag gactggcctc aagtcaaaga tcgtttggaa acatggttgc
    78481 gtcctgaaaa ttttgataaa aatggacagc agtacaagag cttgagagaa ctttaagagg
    78541 tgttgagatg attactatta aaaagcaaga aattgtcaag ctagaggatg ttttacatct
    78601 ctatcaggct gtcggttgga caaattatac ccatcaacca gagatgctgg agcaggcctt
    78661 atctcattca ttagtaattt atctggcact tgatggtgat gctgtggtgg gcttgattcg
    78721 tttggttgga gatggattct catcagtatt ggttcaggat ttaatcgttt taccaatcta
    78781 tcagcgtcaa ggaattggta gtgccttgat gaaagaggct ttagaggatt acaaagatgc
    78841 ctatcaagtc cagctggtga cagaagagac agaaagaacc ttgggcttct atcgttctat
    78901 gggctttgaa atcttatcca cctataattg tatagggatg acttggatga atcgtaaaaa
    78961 ataacaaaac ttgttttttc ttaagcaaaa tttaaggatg gtctagtatt atatagtcat
    79021 taaataaaga cctcctaact ttatttaata aaatcctaaa cttttttcat cacaatctcc
    79081 taatgaagcc acccaatcag gtggcttttt tgcgtggtga ggtgctggtg atagaaattt
    79141 tttgcaaaat agtaaaattt tagaaaagtt aagcta
//
LOCUS       DAVKWS010000003        77729 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKWS010000003 DAVKWS010000000
VERSION     DAVKWS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 77729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.003"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            201..3302
                     /locus_tag="VW852_000223"
     CDS             201..3302
                     /locus_tag="VW852_000223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV0271427.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYLIDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3385..4392
                     /gene="pfkA"
                     /locus_tag="VW852_000224"
     CDS             3385..4392
                     /gene="pfkA"
                     /locus_tag="VW852_000224"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV0271428.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEADFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4451..5956
                     /gene="pyk"
                     /locus_tag="VW852_000225"
     CDS             4451..5956
                     /gene="pyk"
                     /locus_tag="VW852_000225"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV0271429.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6647..6872)
                     /locus_tag="VW852_000226"
                     /pseudo
     CDS             complement(6647..6872)
                     /locus_tag="VW852_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7030..7284)
                     /locus_tag="VW852_000227"
     CDS             complement(7030..7284)
                     /locus_tag="VW852_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0271430.1"
                     /translation="MGTKIGISKNTIGNYEKRVRSTKKNTIFDLVKVFSSLIDALFSP
                     VQKDSPSDIQSIYDQRAPSRQGKVLTYAERQLYDQKNEVS"
     gene            7720..8592
                     /locus_tag="VW852_000228"
     CDS             7720..8592
                     /locus_tag="VW852_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV0271431.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTESTTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8738..9082)
                     /locus_tag="VW852_000229"
                     /pseudo
     CDS             complement(8738..9082)
                     /locus_tag="VW852_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9225..9536
                     /locus_tag="VW852_000230"
     CDS             9225..9536
                     /locus_tag="VW852_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271432.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9540..10256
                     /locus_tag="VW852_000231"
     CDS             9540..10256
                     /locus_tag="VW852_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0271433.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNLYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10386..11201)
                     /locus_tag="VW852_000232"
     CDS             complement(10386..11201)
                     /locus_tag="VW852_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV0271434.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSINITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11198..12103)
                     /locus_tag="VW852_000233"
     CDS             complement(11198..12103)
                     /locus_tag="VW852_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV0271435.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            12501..14630
                     /locus_tag="VW852_000234"
     CDS             12501..14630
                     /locus_tag="VW852_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV0271436.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEILAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14617..15066
                     /locus_tag="VW852_000235"
     CDS             14617..15066
                     /locus_tag="VW852_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV0271437.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            15125..15397
                     /locus_tag="VW852_000236"
     CDS             15125..15397
                     /locus_tag="VW852_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0271438.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15751..15942)
                     /locus_tag="VW852_000237"
     CDS             complement(15751..15942)
                     /locus_tag="VW852_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271439.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16370..17128
                     /locus_tag="VW852_000238"
     CDS             16370..17128
                     /locus_tag="VW852_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271440.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17130..19118
                     /locus_tag="VW852_000239"
     CDS             17130..19118
                     /locus_tag="VW852_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271441.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19160..19804)
                     /locus_tag="VW852_000240"
     CDS             complement(19160..19804)
                     /locus_tag="VW852_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV0271442.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19776..20768)
                     /locus_tag="VW852_000241"
     CDS             complement(19776..20768)
                     /locus_tag="VW852_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV0271443.1"
                     /translation="MLQKANKGKSNREIASLLGKVPQTIHTEIKRRTVRQCLGKGRFK
                     EVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGIST
                     IYYWIHHGKLGLSKQDLPYPRKGKALKKQASTNFKPAGQSIEQRSEAINLRLENGHYE
                     IDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADN
                     GTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEK
                     WINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRNRFKFCRSFKYPARRCSWC"
     gene            21488..22963
                     /locus_tag="VW852_000242"
     CDS             21488..22963
                     /locus_tag="VW852_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV0271444.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINTIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(23086..24216)
                     /locus_tag="VW852_000243"
     CDS             complement(23086..24216)
                     /locus_tag="VW852_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV0271445.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTNKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTRYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNI
                     HAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARFSPAFK
                     AYLKTKLEQGKHYNVAIIHLAKKLIRTLFYILKKSCHLTNKK"
     gene            24303..24440
                     /locus_tag="VW852_000244"
     CDS             24303..24440
                     /locus_tag="VW852_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271446.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            24471..25331
                     /gene="speE"
                     /locus_tag="VW852_000245"
     CDS             24471..25331
                     /gene="speE"
                     /locus_tag="VW852_000245"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV0271447.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            25328..26587
                     /locus_tag="VW852_000246"
     CDS             25328..26587
                     /locus_tag="VW852_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV0271448.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            26587..27714
                     /gene="nspC"
                     /locus_tag="VW852_000247"
     CDS             26587..27714
                     /gene="nspC"
                     /locus_tag="VW852_000247"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV0271449.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            27711..28796
                     /gene="aguA"
                     /locus_tag="VW852_000248"
     CDS             27711..28796
                     /gene="aguA"
                     /locus_tag="VW852_000248"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV0271450.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKDAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFADVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            28806..29681
                     /gene="aguB"
                     /locus_tag="VW852_000249"
     CDS             28806..29681
                     /gene="aguB"
                     /locus_tag="VW852_000249"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV0271451.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(29862..30671)
                     /locus_tag="VW852_000250"
     CDS             complement(29862..30671)
                     /locus_tag="VW852_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0271452.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            30943..31164
                     /locus_tag="VW852_000251"
     CDS             30943..31164
                     /locus_tag="VW852_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271453.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWKMEPVLTLL
                     VHGFCPRDTDDLGYIGGGNHLCKGSAARF"
     gene            31218..31889
                     /locus_tag="VW852_000252"
     CDS             31218..31889
                     /locus_tag="VW852_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0271454.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLLLAVIYLLVFAGKKTFHFKWQLRYFIYLLLGYITSYMSDFLFSYFISLSS
                     NQISLNETIEMIGRQELPYFLLIVCFIGPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNIYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(31940..32137)
                     /locus_tag="VW852_000253"
     CDS             complement(31940..32137)
                     /locus_tag="VW852_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0271455.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            32130..33038
                     /locus_tag="VW852_000254"
     CDS             32130..33038
                     /locus_tag="VW852_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0271456.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLARKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            33035..33496
                     /locus_tag="VW852_000255"
     CDS             33035..33496
                     /locus_tag="VW852_000255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV0271457.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            33486..34373
                     /locus_tag="VW852_000256"
     CDS             33486..34373
                     /locus_tag="VW852_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0271458.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            34376..36259
                     /gene="cbpE"
                     /locus_tag="VW852_000257"
                     /gene_synonym="pce"
     CDS             34376..36259
                     /gene="cbpE"
                     /locus_tag="VW852_000257"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV0271459.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYVVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            36339..37469
                     /locus_tag="VW852_000258"
     CDS             36339..37469
                     /locus_tag="VW852_000258"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV0271460.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            37479..38741
                     /locus_tag="VW852_000259"
     CDS             37479..38741
                     /locus_tag="VW852_000259"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV0271461.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQVLLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADDDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            38745..39542
                     /gene="proC"
                     /locus_tag="VW852_000260"
     CDS             38745..39542
                     /gene="proC"
                     /locus_tag="VW852_000260"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV0271462.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIVGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            39762..40400
                     /locus_tag="VW852_000261"
     CDS             39762..40400
                     /locus_tag="VW852_000261"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV0271463.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            40397..41287
                     /locus_tag="VW852_000262"
     CDS             40397..41287
                     /locus_tag="VW852_000262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV0271464.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            41327..41644
                     /gene="yabA"
                     /locus_tag="VW852_000263"
     CDS             41327..41644
                     /gene="yabA"
                     /locus_tag="VW852_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV0271465.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            41647..42516
                     /gene="rsmI"
                     /locus_tag="VW852_000264"
     CDS             41647..42516
                     /gene="rsmI"
                     /locus_tag="VW852_000264"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV0271466.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            42743..43261
                     /locus_tag="VW852_000265"
     CDS             42743..43261
                     /locus_tag="VW852_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV0271467.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPKNLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            43370..43507
                     /locus_tag="VW852_000266"
     CDS             43370..43507
                     /locus_tag="VW852_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV0271468.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     K"
     gene            43514..44017
                     /locus_tag="VW852_000267"
     CDS             43514..44017
                     /locus_tag="VW852_000267"
                     /inference="COORDINATES: protein motif:HMM:NF018653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV0271469.1"
                     /translation="MSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVK
                     KGIGLIERVRLGLGKPNIIYVKDFMNIFQVKENDLQKSKNLTSEVKDFNLRSKENELQ
                     EVKNLDSNYIENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIR
                     LPAKLES"
     gene            complement(44006..44811)
                     /locus_tag="VW852_000268"
                     /pseudo
     CDS             complement(44006..44811)
                     /locus_tag="VW852_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            44806..45060
                     /locus_tag="VW852_000269"
     CDS             44806..45060
                     /locus_tag="VW852_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0271470.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            45305..46639
                     /gene="trmFO"
                     /locus_tag="VW852_000270"
     CDS             45305..46639
                     /gene="trmFO"
                     /locus_tag="VW852_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV0271471.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            46723..47460
                     /locus_tag="VW852_000271"
     CDS             46723..47460
                     /locus_tag="VW852_000271"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV0271472.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            47469..48026
                     /gene="frr"
                     /locus_tag="VW852_000272"
     CDS             47469..48026
                     /gene="frr"
                     /locus_tag="VW852_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV0271473.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            48086..48940
                     /gene="cvfB"
                     /locus_tag="VW852_000273"
     CDS             48086..48940
                     /gene="cvfB"
                     /locus_tag="VW852_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV0271474.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            48949..49164
                     /locus_tag="VW852_000274"
     CDS             48949..49164
                     /locus_tag="VW852_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV0271475.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            49250..50254
                     /locus_tag="VW852_000275"
     CDS             49250..50254
                     /locus_tag="VW852_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV0271476.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIYQIDFVHFSAKDVVRHPVVAQIIRAYEPAPVKKEESEELESQPLSEG
                     "
     gene            50362..50931
                     /locus_tag="VW852_000276"
     CDS             50362..50931
                     /locus_tag="VW852_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0271477.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            50999..51649
                     /locus_tag="VW852_000277"
     CDS             50999..51649
                     /locus_tag="VW852_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV0271478.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            51633..53873
                     /locus_tag="VW852_000278"
     CDS             51633..53873
                     /locus_tag="VW852_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV0271479.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            54052..54240
                     /locus_tag="VW852_000279"
     CDS             54052..54240
                     /locus_tag="VW852_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271480.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            54272..54859
                     /locus_tag="VW852_000280"
     CDS             54272..54859
                     /locus_tag="VW852_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271481.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            54863..56044
                     /locus_tag="VW852_000281"
     CDS             54863..56044
                     /locus_tag="VW852_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271482.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    56204..56335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            56351..56881
                     /gene="infC"
                     /locus_tag="VW852_000282"
     CDS             56351..56881
                     /gene="infC"
                     /locus_tag="VW852_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV0271483.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            56914..57114
                     /gene="rpmI"
                     /locus_tag="VW852_000283"
     CDS             56914..57114
                     /gene="rpmI"
                     /locus_tag="VW852_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV0271484.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            57166..57525
                     /gene="rplT"
                     /locus_tag="VW852_000284"
     CDS             57166..57525
                     /gene="rplT"
                     /locus_tag="VW852_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV0271485.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            57583..57963
                     /locus_tag="VW852_000285"
     CDS             57583..57963
                     /locus_tag="VW852_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0271486.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            58213..59013
                     /locus_tag="VW852_000286"
     CDS             58213..59013
                     /locus_tag="VW852_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV0271487.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            58976..59962
                     /locus_tag="VW852_000287"
     CDS             58976..59962
                     /locus_tag="VW852_000287"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV0271488.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLKNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            60004..62169
                     /gene="lytB"
                     /locus_tag="VW852_000288"
     CDS             60004..62169
                     /gene="lytB"
                     /locus_tag="VW852_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV0271489.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWIFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWIYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNKNAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLYFDGFKLE
                     NPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(62230..63885)
                     /gene="pavA"
                     /locus_tag="VW852_000289"
     CDS             complement(62230..63885)
                     /gene="pavA"
                     /locus_tag="VW852_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV0271490.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            63990..64487
                     /gene="ybeY"
                     /locus_tag="VW852_000290"
     CDS             63990..64487
                     /gene="ybeY"
                     /locus_tag="VW852_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV0271491.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            64468..64863
                     /locus_tag="VW852_000291"
     CDS             64468..64863
                     /locus_tag="VW852_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV0271492.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            64880..65779
                     /gene="era"
                     /locus_tag="VW852_000292"
     CDS             64880..65779
                     /gene="era"
                     /locus_tag="VW852_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV0271493.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            65828..66652
                     /gene="mutM"
                     /locus_tag="VW852_000293"
     CDS             65828..66652
                     /gene="mutM"
                     /locus_tag="VW852_000293"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV0271494.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFCLTDKVLISHLRMEGKYFYYPDQVPERKHAHVFFRFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            66652..67257
                     /locus_tag="VW852_000294"
     CDS             66652..67257
                     /locus_tag="VW852_000294"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV0271495.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEKKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            67244..68443
                     /locus_tag="VW852_000295"
     CDS             67244..68443
                     /locus_tag="VW852_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV0271496.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTIFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYQAVFYATSLCVAFSCLFNLIRFRT
                     LLKVKEI"
     gene            68443..68592
                     /gene="rpmG"
                     /locus_tag="VW852_000296"
     CDS             68443..68592
                     /gene="rpmG"
                     /locus_tag="VW852_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0271497.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            68632..68865
                     /gene="secG"
                     /locus_tag="VW852_000297"
     CDS             68632..68865
                     /gene="secG"
                     /locus_tag="VW852_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV0271498.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            68967..71321
                     /gene="rnr"
                     /locus_tag="VW852_000298"
     CDS             68967..71321
                     /gene="rnr"
                     /locus_tag="VW852_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV0271499.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            71284..71751
                     /gene="smpB"
                     /locus_tag="VW852_000299"
     CDS             71284..71751
                     /gene="smpB"
                     /locus_tag="VW852_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV0271500.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            71767..72627
                     /gene="tehB"
                     /locus_tag="VW852_000300"
     CDS             71767..72627
                     /gene="tehB"
                     /locus_tag="VW852_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV0271501.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            72700..73653
                     /gene="coiA"
                     /locus_tag="VW852_000301"
     CDS             72700..73653
                     /gene="coiA"
                     /locus_tag="VW852_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV0271502.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKFLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            73672..75474
                     /gene="pepF"
                     /locus_tag="VW852_000302"
     CDS             73672..75474
                     /gene="pepF"
                     /locus_tag="VW852_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0271503.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVVQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREKELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            75476..76189
                     /locus_tag="VW852_000303"
     CDS             75476..76189
                     /locus_tag="VW852_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV0271504.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQRLFDATLDNSELTATLVPLG
                     DGILMLRKNVADVQLPDSE"
     gene            76256..77197
                     /gene="prsA"
                     /locus_tag="VW852_000304"
     CDS             76256..77197
                     /gene="prsA"
                     /locus_tag="VW852_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV0271505.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(77314..77655)
                     /locus_tag="VW852_000305"
                     /pseudo
     CDS             complement(77314..77655)
                     /locus_tag="VW852_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        23473 a        14201 c        17257 g        22798 t
ORIGIN      
        1 agatagggta taaaagttct aggagattta tattatatgc tttctatttt tatatacaat
       61 atagtataaa tataaaaatg atgacaaaaa tacaaatgaa tagaaaataa attagtaagc
      121 tgatgaaatt tttctcaaga gaagccattt ataggtgaaa atggtataat atagtgagaa
      181 ggatagagga gaagtgtaaa ttgatcgcac aactagatac aaaaacagtc tatagtttta
      241 tggaaagcgt catttcgatc gaaaagtatg tgagagcagc taaagaatac ggctacactc
      301 atttggctat gatggatatt gacaatcttt atggcgcttt cgactttcta gagattacaa
      361 aaaaatacgg cattcatcct ttgctagggc ttgaaatgac agtgtttgta gatgatcagg
      421 aagtgaattt gcgcttttta gctctatcta gtgtgggcta tcagcagttg atgaagcttt
      481 cgacagccaa gatgcagggg gagaaaactt ggtcagtcct gtcccagtac ctggaggata
      541 tcgcggtcat tgtgccttat tttgatagag ttgagtcgtt agaactaggc tgtgattact
      601 atataggggt ttatccagaa acactagcaa gcgaatttca tcatcctatc ttacctcttt
      661 atcgggtcaa cgcttttgaa agcagggata gagaagttct tcaagtttta acagcgatta
      721 aagaaaatct accgctcaga gaagttccct tgcgttcgag acaagatgtc tttatatcag
      781 caagttcttt agagaaacta ttccaagagc gttttccgca agctttggac aatttagaaa
      841 agcttatttc aggcatttct tacgacttgg atactagtct gaaactgcct cgttttaatc
      901 cagctagacc agcagtagag gagttgagag agcgtgctga actggggctt gttcagaagg
      961 ggttgactag taaagaatat caagatagac tagaccaaga attgtctgtt attcatgata
     1021 tgggctttga tgattatttc ttggttgttt gggatttgtt gcgttttgga cgatcgaatg
     1081 gctattatat gggaatggga aggggttctg cagtaggcag tttggtttct tatgccttag
     1141 acatcacggg gattgaccca gtagagaaaa atctgatttt tgaacgcttt cttaatcgtg
     1201 aacgctatac catgccagat attgatattg atatcccaga tatttatcgt ccagatttta
     1261 tcagatatgt tggtaataaa tatggtagta aacatgcggc acaaatcgtt actttttcaa
     1321 cctttggagc caagcaagct ctacgagatg tcttgaaacg ctttggtgtt cctgagtatg
     1381 aattatctgc aattactaag aaaatcagtt ttcgtgacaa tcttaagtcg gcctatgagg
     1441 gaaatctcca gtttcgtcag caaatcaata gtaagttaga ataccaaaaa gcttttgaga
     1501 ttgcttgcaa gatagagggc tatccaaggc aaacctctgt ccatgcggct ggtgttgtaa
     1561 ttagtgacca agatttaacc aactacattc ctctaaagta tggtgatgaa attccactga
     1621 ctcagtatga tgctcatgga gttgaggcta gcggactttt gaagatggac tttctgggac
     1681 tacgaaattt gacctttgtt cagaagatgc aagagttgct tgctgaaaca gaaggtattc
     1741 atctgaaaat tgaagaaatc gatttagaag acaaagaaac gttagcttta tttgcctctg
     1801 gtaatacaaa aggtatcttt caatttgagc aaccaggtgc cattcgtctg cttaagcgtg
     1861 tgcaaccagt ctgttttgaa gatgtcgtcg cgactacttc tctaaatcga ccgggtgcta
     1921 gtgactatat caataatttt gtggcaagaa agcatgggca ggaagaagtg actgttctgg
     1981 atccagtact ggaggatatt ttggctccaa cctacggcat aatgctctat caggagcagg
     2041 ttatgcaggt tgcccagcga tttgccggat ttagtcttgg gaaggccgat attttgcgtc
     2101 gggctatggg gaaaaaggat gcctctgcca tgcatgagat gagggcttcc tttattcaag
     2161 gttcattaga agctggtcat actgtggaaa aagcagagca ggtctttgat gttatggaga
     2221 agtttgcagg ctatggtttt aacaggtcac acgcctatgc ctactcagcc ttggccttcc
     2281 agttggctta ttttaaaaca cattatccag ccatatttta tcagattatg ttaaattctg
     2341 ccaacagtga ttacttaata gatgcacttg aagcaggttt tgaagtggcg cctctgtcca
     2401 tcaacacgat tccctatcac gataaaattg ccaacaaggc catctatcta ggtttgaaat
     2461 ccattaaagg agtcagtaat gatttagctc tctggattat tgaacataga ccttattcta
     2521 atattgaaga ttttatagct aaattacctg agaattatct gaaacttcct ctgctagaac
     2581 ctttggtaaa agttggtctt ttcgattcat ttgaaaaaaa tcgtcaaaaa gtatttaata
     2641 acttagctaa tctatttgaa tttgtgaaag agttgggaag tttgtttgga gatgctattt
     2701 atagttggca ggaatcggaa gattggacgg aacaagaaaa attttatatg gaacaagagc
     2761 ttttagggat aggtgtcagc aaacatccac tacaagctat tgcaagtaag gctatttacc
     2821 cgattacccc aatcggaaat ttgtcagaaa atagctatgc tattattttg gttgaagttc
     2881 agaaaataaa agtgattcgt accaaaaagg gtgaaaatat ggccttctta caggcagatg
     2941 atagtaagaa aaaattggat gtcactctct tttcagactt atatcgtcag gttggacagg
     3001 aaataaaaga gggagccttc tactatgtaa aaggaaaaat acaatcacgt gatggccgtc
     3061 tgcaaatgat tgcacaagaa ataagagaag cagttgctga acgcttttgg atacaggtga
     3121 aaaatcatga atcggatcaa gaaatttcac gtattttaga acaatttaaa ggcccaatcc
     3181 cagtcatcat ccggtatgaa gaggaacaga aaaccatcgt ttctccccat cattttgtag
     3241 ctaaatccaa tgaattagag gagaaattga atgaaatcgt tatgaaaacg atttatcgct
     3301 aaaaatacgg aaaatagaag aattttaaaa tcaaatgtga tataatcaag aagaatgtta
     3361 aaagaaaaag gagcataaac caatatgaaa cgtattgctg ttttgactag tggcggagac
     3421 gcacctggta tgaacgctgc tatccgtgca gttgttcgtc aagcaatttc agaaggaatg
     3481 gaagtgtttg gtatctatga cggatatgct ggtatggttg ccggtgaaat tcatccccta
     3541 gatgcagctt cagtaggaga catcatttct cgtggtggta cttttcttca ctcagctcgt
     3601 tatccagagt tcgctcaact tgaagggcaa cttaaaggga ttgagcaatt gaaaaaacac
     3661 ggaattgaag gtgtagttgt tatcggtggt gacggttctt accacggcgc tatgcgtttg
     3721 actgaacatg gcttcccagc tattggtctt ccaggtacaa tcgataacga tatcgttggt
     3781 actgacttta caatcggttt tgacacagcg gttactactg ccatggacgc tattgataag
     3841 attcgtgata catcatcaag tcaccgtcgt acttttgtaa tcgaagttat gggacgtaac
     3901 gctggtgata tcgctctttg ggctggtatt gcaactggtg ctgatgaaat catcatccct
     3961 gaagcagact tcaagatgga agatatcgta gcaagcatca aagctggtta tgaatgtggt
     4021 aaaaaacaca atatcatcgt cttagctgaa ggtgtgatgt cagcggctga atttggtcaa
     4081 aaacttaaag aagctggaga tacaagcgac cttcgtgtaa cagaacttgg acatattcaa
     4141 cgtggtggtt ctccaactgc gcgtgaccgt gttttggcgt cacgtatggg tgcacatgct
     4201 gttaaacttc ttaaagaagg tatcggtggt gttgcggttg gtattcgtaa cgaaaaaatg
     4261 gttgaaaatc caattcttgg tactgcagaa gaaggggcat tgtttagcct tactgcagaa
     4321 ggtaagattg tggttaacaa cccacacaaa gccgatattg agctatctag cttgaataag
     4381 agcttgtcat aatttacaat ttagttaata agaccaaaaa ggtcatatat ataaaggagt
     4441 cacaaaaatc atgaacaaac gtgtaaaaat cgttgcaact ttgggtcctg cggtagaaat
     4501 ccgtggtggt aaaaaattcg gtgaggacgg atactggggt gaaaaacttg atgttgaagc
     4561 ttcagctaaa aacattgcta aattgattga agctggtgct aacacattcc gattcaactt
     4621 ctcacacggc gaccaccaag aacaaggtga gcgtatggca actgttaaac ttgcggaaaa
     4681 aattgcaggt aaaaaagttg gtttccttct tgatacaaaa ggacctgaaa tccgtacaga
     4741 attgttcgaa ggtgaagcta aagaatattc atacaaaact ggtgaaaaaa ttcgtgttgc
     4801 aactaaacaa ggaatcaaat caactcgtga agtgattgcg ttgaacgttg ctggtgctct
     4861 tgatatctat gatgatgttg aagttggtcg tcaagttttg gttgacgatg gtaaacttgg
     4921 tcttcgtgtg gttgctaaag atgatgaaac tcgtgaattt gaagttgaag ttgaaaacga
     4981 tggtatcatc gctaaacaaa aaggtgtgaa catccctaac actaaaattc ctttcccagc
     5041 tcttgctgaa cgcgataacg acgatatccg tttcggtctt gaacaaggta tcaacttcat
     5101 cgcaatttca ttcgtacgta ctgcaaaaga tgtgaacgaa gttcgtgcaa tctgtgaaga
     5161 aactggaaac ggacatgttc aattgttcgc taaaatcgaa aaccaacaag gtatcgataa
     5221 cttagacgaa atcatcgaag cagctgatgg tattatgatt gctcgtggtg atatgggtat
     5281 cgaagtaccg ttcgaaatgg ttccagttta tcaaaaaatg attatcaaga aagtcaatgc
     5341 tgcaggtaaa gttgttatca ctgcaacaaa catgcttgaa acaatgactg aaaaaccacg
     5401 tgcaactcgt tcagaagtat cagacgtatt caacgctgtt atcgacggaa ctgacgctac
     5461 aatgctgtca ggcgagtctg caaacggtaa atacccactc gagtcagtaa ctacaatggc
     5521 tacaatcgac aagaacgctc aagctcttct taatgaatac ggacgtcttg attcagattc
     5581 atttgagcgt aactctaaga cagaagtaat ggcttctgct gttaaagatg ctactagctc
     5641 aatggatatc aaattggttg taactcttac taagacaggt catactgcac gtttgatttc
     5701 taaataccgt ccaaatgctg acatcttagc attgacattt gacgaattga cagaacgtgg
     5761 cttgatgttg aactggggtg ttatcccaat gttgacagat gctccatctt caactgacga
     5821 tatgttcgaa atcgctgaac gtaaagcggt agaagcaggt ctcgttgagt caggcgatga
     5881 tatcgttatc gttgctggtg tgccagtagg agaagctgtt cgcacaaaca caatgcgtat
     5941 ccgcacagta cgttaagaaa aatataaaaa cctatcatat ccagctttct agtctgtatg
     6001 atgggctctt tttttgtcca ggggggcaaa aagggggcaa aactttcata atggattgat
     6061 actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct taccgtactc aagtacagct
     6121 tgcggctagc ttcctagttt gctctttgat tttcattgag tatgaaataa gatatgcaca
     6181 aattgattag gaaattcaaa tgaatttcta caaatgtttt agcaatcgta atgtacttgt
     6241 ctagattcga tctgatatat tttcgattta atgatatggt atttaaaacc tccaaagtag
     6301 cttgctccat tcttttactt acgtgagtgt agatgttatt tactgtttta gcgtttttgt
     6361 gttccactct aaccattata gcattcttct cagctagtgt actaaggagt gtgtgcctga
     6421 aaatatggga actaaggggc tggtttatcg gtttctctag tttagtattt gccttttgca
     6481 aagtgatgtt aaatgccttt ctctaaattt acatatcact attgtttaac aaaatctaat
     6541 ctactttagg tcacttattc tttttttgaa atgtagaatg aactttttca aagtttttcg
     6601 aatcttttaa aatctgtttg ttttatatcg ccattctccc ccttttttaa ttctccctat
     6661 atagcctgac aactttcccg atggtacgaa tatggttgct ttcgtctagg tggatgtcgg
     6721 ggtatttggg attgagtttt tttaaggtag ccttggcgga gtttcttgac atagttagcg
     6781 ccgtctactt ggaagatgcc gatggtatta tagtcaatct gtggggtatt cttgataaat
     6841 aggtagtcgc tgtttcttat ctttggctcc atggacttgc tgatgacata agcgattggg
     6901 tcgtagtcgt ctgggataat ggaaactcca tatctaaatc gttgtcctgc atcgagcggc
     6961 tacctgcaga gataaactat ctaacacgag agtaagtagt ctgtctgtag tcgtccagtc
     7021 tgatgatttt tacgatactt cgtttttctg atcatacagt tgcctctcgg cataggtcag
     7081 aactttacct tgtctggatg gtgcccgttg gtcgtagata gattggatat cgctaggaga
     7141 atccttttga actggagaaa agagggcatc aatcaagcta ctgaatactt taactaagtc
     7201 aaatatagta tttttcttag tagacctaac ccttttttca taatttccaa tggtgttttt
     7261 acttatacca atcttagtac ccaattctta ttgagttcaa ccattactag tctatattgt
     7321 tttatagttg attgagtttg gaatagtacg ctgtagctgc taaaacattt ctaggaatta
     7381 atttgccttt cctaatagag ttgttcatat cttatttcaa tctactatat ttttcacctc
     7441 taacaatcgc aatctcttct ttatccatga ataaaaacga tttctatttt tgtaagtaaa
     7501 gcatcacacg aaatccacga aaatgaaaac ctttgttgtg ttttcgtaaa aaatttgttg
     7561 acagagcacg aaacgcctac tataattaaa tcaagtttaa ggaaataaca aaaaaacaaa
     7621 tcggagggaa agacgatgaa cacattaaat gagaaagtaa tcaatatctg taaagcagta
     7681 gttaaagaaa ctttaatcca agacatttag gagaaagaaa tgaagaaatc aagaaaatta
     7741 gccacattag gaatctgttc agctttgttt ttaggcttgg ctgcttgtca acaacaacat
     7801 gctacttctg aggggacaaa tcaaaggcaa agcagttcag cgaaagttcc atggaaagct
     7861 tcatacacca acctaaacaa ccaggtaagt acagaagagg tcaaatctct cttatcagct
     7921 cacttggatc caaatagtgt tgatgcattt tttaatctcg ttaatgacta taataccatt
     7981 gtcggctcaa ctggcttatc aggagatttc acttccttta ctcacaccga atacgatgtt
     8041 gagaaaatca gtcatctctg gaatcaaaag aagggcgatt tcgttgggac caactgccgt
     8101 atcaatagtt attgtctttt gaaaaattca gtcaccattc caaagcttga aaagaatgac
     8161 caattgcttt tcctagataa tgatgcgatt gataaaggaa aggtctttga ttcacaagat
     8221 aaggaagagt ttgatattct attttcgaga gttccaactg agtcaactac agatgtcaag
     8281 gttcacgctg aaaagatgga aacattcttc tcacaatttc aattcaatga aaaagctcga
     8341 atgctgtctg tagtcttgca cgacaatttg gatggcgagt atctgtttgt aggccacgtt
     8401 ggggtcttag tacctgctga tgacggtttc ttatttgtag agaaattgac tttcgaagag
     8461 ccctaccaag cgattaaatt tgctagtaag gaagattgct acaagtattt gggcaccaag
     8521 tatgcggatt atacaggcga gggactggct aagcctttta tcatggataa tgataagtgg
     8581 gttaaacttt aatagtgtta tgatatgaca gtaatcttag gatttttatt tctatttttg
     8641 atgacttggt gggttatttc aatcgttaat agccagaaac cacgcaaaaa tgaaacattt
     8701 attggctata tccaaagata cgatattgat gggaatggct atcaatcatt accgtgtcct
     8761 cagaactgag aggagttctt gaaatcgtaa aaccactttg aacaagagtt acttcaaccc
     8821 attggctctg atggattaag ttgttttcgt gaataccaaa atcagccgca atttcttcat
     8881 aagtgcggta ttctcgcaca tattgaagag tgggcataag aaggtcttct aggcttaatt
     8941 taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat acagctaata
     9001 tctcttcaaa agtcgtgcgc tgaacaccaa caagacgctt aaatcgtgca tcagttagtt
     9061 gtttacttgc ttcataattc atagaactac tataccatat tttgtttcgc aggaagtcta
     9121 atgctaaaat cttatcagtg agaagatttc ttaaagttta ttctttgtag tttcatctgt
     9181 attagtacat aatttaactt agagaaaata ggaggactaa tcgaatggaa ttaaaagatt
     9241 ttacagaaaa agaacaggaa atgattaaga aaaggcttac aatgtctaat attagtgaca
     9301 aagaaactac tgagaagatt ctggcgctag taccacaaga cttgattaag cgaatcccgt
     9361 tttttgtcag aaaacatgct acaacacgta cgattaaacg catttcaatt gaacaccctg
     9421 aactctacgc tgtagctcaa acaagtggtg agattccaga aaaagaacgc gaagaattgc
     9481 gtcagattat cacgactatc tttgaacaaa agatgaataa gcatagtatt aagtagagaa
     9541 tgaaaaaata ttttattggc ggtttgggaa ataatgccta tcatagcaag gattttcttc
     9601 aagaactaga ttcgcaggtc tattttctaa atctatatga aaagcatctt cgagatgaaa
     9661 cagaattgaa atcatggttt aaaaatgaga ttgtagagga agaatctatc tgtctgatag
     9721 gtcattctct tggaggagat ttagctcgtt atttcgcatc ggaatttgaa gaagtaaaga
     9781 aactgattct tttggatggt ggctatctag atttagataa gattttacct ttggatacag
     9841 agttagagga aactaaaaat tatatcaaat ctcaaattgt ttcggactta gctcttctta
     9901 tttctaaaga gaagtccgaa gcaaagcatt ggtcagaaaa tatggagaaa gctgtaagac
     9961 agtcctatca ctggaatgtt gagtataata gatatgagtt ggctataaat tatgaaaata
    10021 tagaagcgat actccgccta cggagaaaaa tacaagcttt taagagagaa gtgggagata
    10081 ccttgtttat tagtcctcgc tatcctaatg gagctacatg gagagaggaa gccctaaaag
    10141 aattgccaga ctattttgat actatttttt tagaaaactt tggccatgag ctttatactc
    10201 aagcacctaa agaaatcgct agtctaatga atgagtggct cgcttatttt ctatgagcta
    10261 cttgtattgc tggaaatggt ataaggtgaa aatctacaag tcactaattt ttatataaca
    10321 aaaaaacgaa caggctcttt ttctgttatt cagaagagaa cttgttcgtt tttatcttga
    10381 ttttcttaaa aagctctata tacataggga ttttctggtt tatcgacttt ggtaaagcta
    10441 tcgatttcca aggtcgggag attttgtttg agttctttat ggtagtgatt gaccagtttt
    10501 cctttggctg ttatccaagt gttgttctca tattgacggg tatttccctt ggtcagcaac
    10561 ccatagacac cagaatctgc gatgcagtga ataatgccga agcggaacag aaattggcta
    10621 ttggcatgac tggggtcatt atagacaaag cctgtgaact ggattgtctt accctcaaat
    10681 tcatctggat agtcgtagat agcctccatg acctccatat agttttcgtt agtaatgtta
    10741 atgctgtctt gggataagta tttatccgcc gccgttcgca tttccttttc ataggctgat
    10801 tttgaaaaat aagaactggt atctggtttc aaatattggc ttgtcgtccc ttcgcttgtc
    10861 tgaatggcta gatctgttcc ttccgatagg gggaaatgat aacctttagc agaaacggtc
    10921 tgagaatcca agctaacagt tgggaaagtt aagccgatga caagtggaat agccagaaga
    10981 gaaatactcg tcatcttggc caatcggctg ttcagatgac tgtgggtttt gacttgcttc
    11041 atccagatat acaattgaac aattgccaag ataaaggaaa gcaccatgga gatataggcc
    11101 agataggaat agtgcatgtt gatgtactgg tttagtttgc ccgacagatg gaggtaaata
    11161 gtcagttcaa aatagccagc taaaactaaa aatcgaatca taggatgact ccaatcaaga
    11221 gagaatagac taagacgaca agagttacaa tcgttatgaa gtgactgata aatcgtgctt
    11281 tcaagtaatt tttcatcatg agaatatttt tgatatccag cattggacca atgacgagaa
    11341 aggccagaac tggtgccaaa ccgaaactcg agagaagaga agcacctata aaggcatccg
    11401 cctcactaca gagcgaaaga agaaaggcta aaagcatcaa gagcaggata gcaaaaagag
    11461 gagtcgcact gatagaggtc agaatccgag tcggaacgta gacctgtatt atagaagcaa
    11521 agaggcagcc aaataccaaa taacgccccg tatcaaaaaa ttcatcaatg gcctgcacaa
    11581 agacttgaaa aacttttttt gcagaactca agtaagaaaa atcatgctca tgacaagcca
    11641 gacgattttc tttctgaatc ggttcttgcc agaaaaatcc tagaaaaatt cctagtatta
    11701 cagccacaag aatggaaccc agagctcgta atagggcgac atggaaggag ttgccaaagg
    11761 cagaataggt cgcaaaaaga acaatgggat tgataacagg tgctgtcaca agaaaaggaa
    11821 cggccgtgta acttggaacc tttttttcca gaaaacgatt gatgatgggg acgattccac
    11881 attcacagga agggaaaagt ataccgacaa aggtcccaaa aaagattctc ccccaacgat
    11941 ttcgagggag aaaatgataa accttgtcag gtgtgatata aacttcaatc agccctgaga
    12001 caatgcttcc tatcagaacg aagggaaggg cttcaatgat aatggagaga aaaatggctc
    12061 cagtttgaag tacactagat gggagttgtt gaaaagcagt catctagttt ttcttttctg
    12121 ctttggcttt tttctctttg tcatcaggga aagtgacttg tttcaaatca gggagtttag
    12181 caaactcttc aaacatcttg tccaagtcgt ctcgttttgt aaatttgaca gccataggtg
    12241 tacctcgatt ctaaattcta cctctattat actattattt tgcggaaaag ggagtttttt
    12301 aatttgtttt cgatagttga tacacttcga aaatctcttc aaaccacgtc agcttcgcct
    12361 taccgtatat atgtgactta tccacaacct caaaacagta ttttgagcag cctgcggcta
    12421 gcttcctagt ttgctttttg attttcattg agtatgaaaa ggattggaga aagccttcca
    12481 gcttgtggta taatagattt atggataaaa aatatgaaaa aatctctcag gatttgggag
    12541 tgatgttaaa gcaaattgat accgttctaa gtttgacagc tgaaggggcg actattccct
    12601 ttatcgcgcg ttatcgcaag gacatgactg gtagtctgga tgaggtggcg attaaggcta
    12661 ttattgattt ggataaaagt ctgacaaatc tcaatgaccg taaggaagct gtcttagcta
    12721 agattcaaga acaaggtaag ttgaccaagg aattggaaga agctatctta gttgccgaaa
    12781 aattagcaga cgttgaagaa ctctatcttc cttataagga aaagcgtcgt accaaggcaa
    12841 ccattgcccg tgaagctgga ctctttcctc ttgctcgttt gattttgcag aatatagttg
    12901 acttagagaa agaagctgaa aagttcgtct gtgaaggatt tgcgactggc aaggaagcct
    12961 tgaccggtgc agttgatatt ttggtcgaag ccttatcgga agatgtgacc ttgcgttcta
    13021 tgacttatca ggaagtgctg agacactcta aacttacttc tcaagccaag gatgaaagtc
    13081 ttgatgaaaa gcaggttttt cagatttatt atgatttttc agagacagtt ggaactatgc
    13141 aaggctatcg taccttggct ctcaatcgtg gggagaaact tggtgtcttg aagatcggtt
    13201 ttgaacatgc gacggaccgt attcttgcct tctttgctac tcgtttcaag gtgaaaaatg
    13261 cttatattga tgaagttgtt cagcaatccg ttaagaaaaa ggtcttgcct gctatcgagc
    13321 gtcgtattcg gacagaatta actgagaaag ctgaagaagg agctatccaa cttttttctg
    13381 acaatctgcg caatctcctc ttggttgctc cactgaaagg gcgcgtggtt cttggatttg
    13441 acccagcctt tcgtacaggt gccaagttag ctgtcgtgga tgcaacagga aaaatgctga
    13501 caactcaggt tatttatcct gttaaaccag catcagctcg tcaaatcgaa gaagccaaga
    13561 aagatttagc agatttaatt ggtcaatacg gtgtagagat tcttgccatt ggaaatggaa
    13621 cggccagtcg tgaaagtgaa gcttttgtag cggaagttct gaaagatttc cctgaagtca
    13681 gctatgttat cgttaatgaa agtggtgctt ctgtctattc tgccagcgaa cttgctcgtc
    13741 aggagtttcc agacttgacc gttgaaaaac gctctgccat ttctatcgcc cgtcgtttgc
    13801 aagatcctct tgcggaattg gtcaaaatcg atcctaagtc aattggtgtc ggtcaatacc
    13861 aacacgatgt cagtcagaag aaactatctg agagtctgga ctttgttgtc gatacagtgg
    13921 ttaaccaagt tggtgtcaat gtcaatacag ctagcccagc tcttctttca cacgtagctg
    13981 gactcaataa aaccatttct gaaaatatcg tcaaataccg tgaggaagag ggaaaaatca
    14041 cttcacgctc ccaaatcaag aaagttcctc gtttgggggc caaggctttt gaacaggctg
    14101 ctggtttcct ccgtatccct gaaagtagca atattcttga taatacagga gttcacccag
    14161 aaaattacgc tgccgttaag gaactcttca aacgcttgga cattaaggac ctgaatgaag
    14221 aagcccaaag caaactcaag tccctttcag tcaaggaaat ggcgcaagag ttagaccttg
    14281 gtccagaaac tcttaaagat atcattgcag atcttctcaa accaggtcga gatttccgtg
    14341 attcctttga tgctccagtt ctccgtcaag atgtcctaga tatcaaggac ttagtggttg
    14401 gacaaaagct agaaggagtg gtgcgtaacg ttgttgattt cggtgctttt gttgatatcg
    14461 gaattcatga ggacggtctc atccatattt cccatatgag tcgcaagttt atcaaacacc
    14521 ctagtcaagt ggtggcagtt ggagatttgg tgagcgtttg ggttaagaaa attgatactg
    14581 aacgtgaaaa agttaatctg tctctcctag ctccaaatga aactgactga ttacgtcaag
    14641 caggtttcac tagaagactt cggcagacct tttatccatc atgtccagtg gaataggcgt
    14701 ctacgttcga caggtgggcg atttttcccc aaagatgggc atttggattt taatcccaag
    14761 gtttatcagg aactggggtt ggacgttttt aggaaaattg tccgacatga actctgtcat
    14821 tatcacctct attttcaagg gaaaggctat caacataagg atcgagattt taaggaactg
    14881 ttgaaagcag tggatggatt acggtttgta ccatccttgc ccaatagtaa ctccaagcca
    14941 atcaagctct atcgttgcca atcctgccag caaacttatc agcgtaagcg aagaattgat
    15001 accagacgct atcgctgtgg actttgtcga ggtaaattgc tactaataaa tcagcctgag
    15061 gactgatgaa agccgagcct acccgtgata tactatgtct aacctagcag aaagaggaaa
    15121 tatcatgaac agtaaatttt ataaaatgag acgaaatcgc atgatttcag gagttttggc
    15181 aggcctgtca gacaagtgga actttgatgt tacccttgta cgttttctgt ttgccatttt
    15241 tacagtcgca aactttggac ttggggtgat tatctatatc atcctagctt ctatcatgcc
    15301 aaccaaggaa gaaatcgaag cagaaatgta cggaacaggc ccacgtaaac gcaaagaagc
    15361 tcaagccatt gacgataatg aaggctggtt ttggtgaggt tttacaaaag atttggaata
    15421 cgcaaaaagc tttaaatcaa tagtctaaac tgcttgattt aaagcttttt tacttttata
    15481 ctcaataaaa atcaaagtgc aaactaggaa gccagccgca ggctgtaatt tagtcggcaa
    15541 tgtgaagtta acatagtttg cactttgatt tttgaatagt attaataata tttcgtcagc
    15601 ttcatccaca aactatagtg gattggaact agaatagtac acctctactt ctaaaatatt
    15661 gttagaaatc gatttgactg tcctgatcga tttgtcctgt tcttatttca ttttactata
    15721 aaagcagtaa tttaaacgca gttttttaat ctatttttgc tgttgagaca atacattttt
    15781 ggaaaaatgg tggatgctga taacaactga taaccagcaa ataacataag cagtgccggt
    15841 aaattctgta ctgataaagt gtgtgtgttc aactaagagc tgaccgatga atggagagag
    15901 cagatagaga agtccgatga gtatatgttc tttgtgtttc attaaagcgc accttctttc
    15961 cttataggtt gaacgacttg caagtgttaa attgaatggt atgttatcta ggtaagctta
    16021 ctaaaatgtt agttcccgct tcctcctttt caaagtaagc cttttcacac ttatatttta
    16081 caactttaaa aataggaaat caaatcattt tctaagtaat tttgcttata tttcacccaa
    16141 tcttacaaaa atgtaagatt aaagtctctt ttgtaaggtt aggttatggc aagcggtctt
    16201 tttttgatgt aaactagact cataaagaac aaaggagtaa catcataaaa caaatcttac
    16261 ataagaaaac tagaaaacca agtcacaaag aaattgagcg cattcagctg ggatgcacta
    16321 tgatgcaagc tacatttcat ttgatgggat attaagaaag gagattctca tgacactttt
    16381 agatgtaaaa cacgttcaaa aaatttataa aactcgtttt cagggcaacc aagtagaggc
    16441 cctcaaggat attcacttca ccgtagaaaa gggtgactac gttgctatca tgggtgagtc
    16501 tggttctggt aaatccactc tgctcaatat cctagctatg ctggataaac caagtcgtgg
    16561 gcaggtttac ctaaacggaa ctgatacagc aactattaaa aattcacagg cttctagttt
    16621 tcggcgtgaa aagctaggat ttgtcttcca agactttaac ttgctagata ctctgtctgt
    16681 taaggacaat atcttgcttc cgcttgtctt gtcaagaaga cctataacgg agatgatgaa
    16741 gaaattggtg atgacagctg aaaatctggg tatcaaccaa ttgcaagaga agtaccctta
    16801 cgagatttct ggtggtcaga aacagcgtgt agcagtagcc cgcgccatca tcacagaacc
    16861 tgaaattctc cttgcggacg agccaacagg agcccttgat tccaagtcat ctgcagcctt
    16921 acttgatgtc tttaatgaaa tcaatgagcg tgggcaaacc atcctcatgg taacccactc
    16981 aacagcagct gctagcaggg ccaagcgtgt tctctttatc aaagacggca ttctttacaa
    17041 ccaaatctac cgtggagaga agacagagcg tcagatgttc caagaaatct ctgatacctt
    17101 gactgtcatg gcaagcgagg tgaattagta tgtttcgatt aaccaataag ttagcagtat
    17161 cgaacttgat taaaaaccgc aaactctact atccctttgc actggctgtt ctcttggcag
    17221 tcaccatcac ctatctcttt tactccctaa ccttcaatcc caagattgcg gaaatccgtg
    17281 gaggaaccac cattcaagca acacttggat ttggtatgtt tgtcgttacc cttgcgtcag
    17341 ccattatcgt cctctatgcc aatagttttg tcatgaaaaa ccgttccaag gaactgggta
    17401 tatatggcat gttaggcttg gagaagcgcc atctaatcag tatgaccttt aaggagttag
    17461 tgctatttgg gattctaact gttggagcag gaatcggtat tggagccttg tttgacaagt
    17521 tgattttcgc tttcctactc aaactgatga aattgaaggt ggaactggtc gctaccttcc
    17581 agatgaaagt tgtcattaca gtacttcttg tcttcggttt gattttccta ggcctcatgt
    17641 tcctgaatgc ccttcgaatc gcccgtatga atgccctcca gctctcacgt gagaaagcta
    17701 gcggagagaa aaaaggtcgc ttccttcctc tccaaacgat tcttggttcc ataagtttag
    17761 ggattggata ttatcttgcc cttacggtaa aagatcctct tacagcctta acaaccttct
    17821 tcatagctgt tttactggtt atctttggga cttatctctt gttcaatgca gggattaccg
    17881 tttttcttca aatcttaaag aaaaataaga aatactatta ccaacctaat aatctcatat
    17941 ctgtttctaa cttgattttc cgtatgaaga aaaatgcagt tggactagca actatcgcta
    18001 ttttgtcaac aatggttttg gtaaccatgt cagcagcgac aagcattttc aattccgcag
    18061 aatcctttaa aaaagttcta aatcctcatg attttggggt ttcagggcaa aatgttgaaa
    18121 aagaagattt ggacaaactc ttgagccagt ttgcaagtga caaaggttat aagattaaag
    18181 agaaagaagt gcttcgttac acttactttg ctgttgcgaa tcaagaagga accaagttaa
    18241 ctatttttga aaaaggacaa aaccgtgtcc aaccaaaaac agttttcatg gtatttgacc
    18301 aaaaagatta tgaaaatatg actggtcaaa aactgtctct atcaggaaat gaggtcggtc
    18361 tctttgccaa aaatgacgga ctgaaaggac agaaagctct aactctaaat gatcatcaat
    18421 tttctgtcaa agaagaattt aataaagatt tcattgtcaa ccatgttcca aataagttta
    18481 atatcttgac tactgattac aattaccttg ttgttcctga tttacaagcc tttttggatc
    18541 aattcccaga ttcggctatc tataatcagt tttacggtgg tatgaatgta gatgccagcg
    18601 aagaagaaca actcaaggtc gctgaggagt atgaaaacta cctcaatcaa tttaatgctc
    18661 aattagatac agaaggtagc tatgtttatg gtagcaatct agcagatgct agttctcaga
    18721 tgagtgccct ctttggtggt gtcttcttta tcggtatttt cctatccatt atctttatgg
    18781 tcggaactgt tctggtcatc tactacaaac aaatttctga aggctacgaa gaccgtgaac
    18841 gctttattat cttgcagaaa gtcggtttgg accaaaagca aatcaagcaa accatcaaca
    18901 aacaggtttt aactgttttc ttccttcctt tgctctttgc cttcatacat ctcgcctttg
    18961 cctaccatat gcttagcctg attttaaaag tgattggtgt actggatacg actatgatgt
    19021 tgattgtgac cttgtctatc tgcgctatct tcctcatcgc ctatgtgctg attttcatga
    19081 ttacttcaag aagttatcgc aagattgtgc aaatgtaaaa aagatacctc gacttcaaaa
    19141 tcgaggtatt tcttgtattc taaatgctga aaagttgttc gagcaggaag gtaactccca
    19201 tggtcaagag accaatagca aggttccgaa tcatagctgt tctagtcgga tcctttccaa
    19261 gtctggcact agtgtaacca gtgagaagaa gggccacacc gacaataagg acagtagcag
    19321 ggatgcgata ttcacttggg aatatggtca ctgacagcat tggaggcaaa cttctaagga
    19381 aaaaggcaac gaagctagaa atggcagcgt gccaaggatt ggtaaattct tcatactcaa
    19441 tcccatattt ttcctctacc agagccttga gtggattttt aagaaagatc ttattggtca
    19501 agagttgggc agaagttttg cattctccat tttggatata agcagcatag agggattttt
    19561 tggctagttc cctatcttgg tctaacaaga gtttttctcg cgaaacggca gcttcctcgg
    19621 tatcttttgg agttgaaacg gatacatatt ctccaccagc cattgaaaag gcaccagcta
    19681 agatagccgc aaatcctgat aaaaagataa tccagatatt ggtcgtggca ctggcaaccc
    19741 cgataaccac accagcaatg gaaataattc catcgttagc accaagaaca cctgcgcgca
    19801 ggatatttaa acgacctgca aaatttgaat cgatttcgtg atttgtttct gacgctaaat
    19861 ttcaagttca agttagccat ccagaagtct tctctgggtg acttgtagtc caagcatttt
    19921 ttaggatagt tgttaatcca cttttcgatg aatgcgactt ctttgggagt cattttcttg
    19981 gttcccttag gtaaccatct acgaatgagc ctgttgtgat tctcattagt tcccctttcc
    20041 caagaggcat aggggtgcgc ataatagatg tgctcctcag aaaatacatc aaacaagcga
    20101 ttgaattccg ttccattatc tgccgtgatg gaaagaatct tgtgttgttt taagatgagt
    20161 tttagagcct gattgaccac ctcagcactt ttatttggaa tcaatcggat gatctgatgt
    20221 ctactctttc gatccgtcaa gacaagcaag cagtagtttt tcgctctcgt aagtagaacc
    20281 gtatcaatct cataatgccc attctccaag cgaagattga tagcttcaga ccgctgttcg
    20341 atagattgac cagcaggttt aaagttggtg ctagcctgtt tcttaagcgc ttttcctttt
    20401 ctagggtaag gcaaatcctg cttgcttaac cccaattttc catgatgaat ccaatagtaa
    20461 atggttgaaa ttcccacgtt aaccccttta gccataacca tcatttcagg cgaaaatttt
    20521 tggttatgat agtggagaat cttttccttt agttccttgg tcaagcttga tttcttgacc
    20581 gagcgcttgc gattgttttc ataagactgt tgagcatagt cggcagaata aacctctttg
    20641 aagcgccctt ttccaagaca ttgtcggact gtcctacgct tgatttcagt gtggatagtt
    20701 tgaggaactt ttccaagcag agaggcaatt tctctatttg atttcccttt gttcgccttt
    20761 tgtagtataa tggtcttgca tctctgcgcc tttcttgtgt ttgtggttga acaacaagta
    20821 taacacagag gtgttttctt atgcctacaa gagctatcgg ctagttgaac catctaattt
    20881 ttaggagggc tgggtggcta acttcattat agaactttca tttacgaaca tatagtaaaa
    20941 tgaaacaaga acagaacaaa tcgatcagga cagtaaaatc tatttctaac aatgttttag
    21001 aagcagaggt gtactattct agtttcaatc tactatattt ttatttttta tcaaaaaata
    21061 ctttacaagt tcttaaaaac atgatatagt aataaagctt agaaaatgag atgatgtttt
    21121 ctagcaaata taaacccgag taaaaaatgc ctacggacag gcagggttga atgccgaagc
    21181 gtggttgaaa agccacatta ttgatagggt taaaagccta cttttataag ttgatgttag
    21241 gacacttgtt ctaattcata aatttttagt gtggtgaaag cacacgtcat cttgtgaaac
    21301 gatcaataaa gtacgtaata tttgctacta gagagttagg aaacatcggg aacagacata
    21361 ctcaacagaa accaaaataa acacgtcaga agattgcaga gcaggtgaaa acctgctctt
    21421 ttttcatgag tcaaccttta gttccttagt tttcataagg tcctaaaaat attgaaagga
    21481 gtatgttttg aaagagttag atcaaaacca agccccaatt tatgaggcct tggtgaagtt
    21541 acgcaagaaa aggattgttc cctttgatgt tccaggtcac aagcgtggac ggggaaatcc
    21601 agaacttgtc gaactcttag gagaaaaatg tgtaggcatt gatgtcaatt cgatgaaacc
    21661 cttggataat ttaggccatc ctatttcgat tattcgtgat gcagaggagc tggctgcaga
    21721 tgcttttgga gctagccatg cctttctaat gattggtgga acaacttcat cggtgcagac
    21781 tatgattctg gcaacctgca aggcaggaga taagattatt ctgccacgaa atgtccataa
    21841 atctgctatc aatgcgttgg ttctatgtgg tgccattccc atctatatcg agatgagtgt
    21901 agatcctaag attggtatcg ctttaggtct tgaaaatgac cgagtagcac aggccataaa
    21961 ggaccatcca gatgctaagg ctatcctaat caacaatcct acttactacg gcatctgttc
    22021 agacctaaag gggttgacag aaatggctca tgaagctggc atgatggttt tagtagatga
    22081 agcccatgga gcgcatttgc atttcactgg taaacttcca atttctgcta tggatgcagg
    22141 agctgatatg gcagcagttt ccatgcataa gtctggtggg agtttgacac aaagttcgct
    22201 tcttttaatc ggggagcaga tgaatcccga gtacgttcgt cagattataa acctgaccca
    22261 gtctacatct gcctcttact tattgatggc tagtttggat atttcacgtc gcaacttggc
    22321 ccttcgtggt aaagagtcgt ttgaggaagt cattgagcta tccgagtacg ctcgtcatga
    22381 aatcaatacc attggcggtt actatgctta ctcaaaagag ttaatagatg gtgtgtcggt
    22441 ctgtgatttt gatgtaacca agttgtcagt ttacactcag ggaataggct taacaggtat
    22501 cgaggtttat gacctcttgc gagacgaata cgacattcag atcgagtttg gtgatatcgg
    22561 caatatcttg gcctatattt ccatcggcga ccgcatccaa gacatcgagc gcttggttgg
    22621 tgctctggct gatattaaga gactctattc aagagatgga aaagatttga tagcaggaga
    22681 atatattcag cccgagttag tgctgtctcc gcaagaagcc ttctattcag agagaaaaag
    22741 tttgaccttg gacgaatctg ttggacaggt ctgtggggaa tttgtcatgt gttaccctcc
    22801 aggaatccct atcttggctc ctggcgaacg catcacacga gaaattgtcg actatatcca
    22861 atttgccaag gaacgtggtt gctccctcca agggacggaa gatccagagg tcaatcacat
    22921 caacgtcatt aagagaaaga caaactataa gaaaagtcaa tagttttatc taaactattt
    22981 cttatttcaa tttgatgatt tggcgatgat tttagagcac ggcaaaaagc ccttgaaaaa
    23041 gtccattttt tcaaaggtaa tcctgtgtta atttcagaaa ttacatcact ttttgttcgt
    23101 caaatggcag ctctttttta ggatataaaa cagggttcgg ataagttttt ttgcaaggtg
    23161 gatgatggct acattgtaat gttttccttg ttctaactta gtcttaagat aggccttaaa
    23221 agcaggtgaa aagcgagcgc atgctttggc agcttgtatg agtgcccacc gcagatgagg
    23281 ggaaccccgt ttgatcattc ttccagctaa atcaatctga cctgactgat aaatagaaga
    23341 atccagtcca gcgaaagctt gtaattgagc aggattatca aaggcatgaa tatttcgaat
    23401 ctcggctaaa atgaccgccc ctaaacgacc cccaatccca gtaaccgtcg tgatgaccga
    23461 gttgaactca gccatcaagt cattgacaca tgtttccgcc ttgtcaatga gcctcttgta
    23521 atgtttgatg atttcgatga tttcgaattc acgagcagga gatgttgttc cgatagaacg
    23581 aggtgcgact gagaggatat cctgaatttt agaagcggtc aatcgcttaa tttctatcag
    23641 cttatcaaat cctgcctcaa gccttttctg aggattaggg tagcgtgtca agagttggta
    23701 ggtatattct gaatgctttc caacgatttt atccaactca ggaaagatga tatcaagaca
    23761 acgagtgtat tgtactttcc aatcagactg ttttttcttg agacgatgaa tatgtctagc
    23821 cagtattttt agttctactt gccgattatc gtgttgaaat tgttcacgat tggggtcaga
    23881 aagaagttta agagcgatgc catgagcgtc tttcttattc gttttagtct tgcgaagtga
    23941 taatgatttg gcaaattcct tgatgagcaa aggagtgtag gtgtaaactt tatatccttg
    24001 ttcatgcagg aagttcagta gattaaaggc ataatgtccg gtattttcaa gagcgatgag
    24061 acagtcttgg ttgagctgtc gaagagacag atctaagagt tcaaaaccag ctttattatt
    24121 tgaaaaagtg agtggtttaa gaacagtttt tcctggaaca ttcaaggctg taacatcgtg
    24181 tttattttta gcgatatcaa tgcccacata aagcatggga gtacctccag atatagtatt
    24241 tcaagtctac ttggttatcc acgaattttt tgccttgtta ccttagacga gatcaaacgt
    24301 ctatgcgtta tcaaactcat taccaattga aacaaaagct gtggttagag cctttcggaa
    24361 atcgtcaagc gattggagga aatgaactaa tccacagtgg cttattccaa gtataccact
    24421 tgggctttgg cagtagctaa ctgcgctaaa tataatataa ggagaaatag atggatttat
    24481 ggttttctga agttcatact ccagatgtca aattgtctct gagaacagcc aagcaacttt
    24541 acgctggaaa aagtgaatgg caggatatcg aagtcttgga tacgccagct tttgggaaaa
    24601 tactgatttt aaatggccat gtcttgttct cagatgcgga tgatttcgtc tacaatgaaa
    24661 tgaccgttca cgttcccatg gctgtccacc caaatccaaa gaaagtattg gttattgggg
    24721 gtggtgacgg cggtgttgcc caagtattaa cactctatcc tgaactggaa caaatcgata
    24781 tcgtggaacc ggatgagatg ttggtcgagg tctgtcgtga gtatttccca gactttgctg
    24841 caggcctaga tgatcctcgt gttaccattt actaccaaaa tgggctacgc tttttgcgaa
    24901 actgcgaata tgactatgat attatcatca acgatgcgac agatccattt ggccatacgg
    24961 aaggactctt taccaaggaa ttctacggca atagttatcg agctctgaag gaagacggca
    25021 tcatgattta ccagcatggg agtccgttct ttgacgagga tgagtcggcc tgccgaagca
    25081 tgcaccgcaa ggtcaatcaa gcctttccaa tcagtcgggt ttatcaggcc catattccaa
    25141 ctagcccagc tggctattgg ttgtttggat ttgcatcgaa aaaataccac cctgtcaaag
    25201 attttgacaa ggaaggctgg aaaaaacgcc agcttttcac agaatactac actgcaaact
    25261 tacacgtggg agcctttatg ttgcccaagt atgttgagga cattttagaa gaagaggaag
    25321 gaaaaaaatg agtcgtttac tagttattgg ttgtgggggc gttgcccaag ttgctatttc
    25381 aaagatttgt caagatagcg aaacatttac agagattatg attgctagcc gtaccaagtc
    25441 aaaatgcgat gacttgaaag ctaaactgga aggcaaaaca agtacaaaga ttgagacagc
    25501 tgctcttgat gctgacaagg ttgaagaagt gattgctctg attgaaagct acaaaccaga
    25561 agctgttttg aatgtagctc tgccttatca agatttaacc attatggatg cttgtttggc
    25621 aacaggtgtt cactatatcg atacagccaa ctacgaagca gaagacacag aagaccctga
    25681 gtggcgtgct atctacgaaa aacgttgtaa ggaacttggt tttacagcct actttgacta
    25741 ctcatggcag tgggcttatc aagagaaatt caaagaagca ggcttgactg ctcttcttgg
    25801 ttctggtttt gacccaggtg taactagtgt cttttcagct tatgccctca aacactattt
    25861 tgatgaaatc cattatatcg acattttaga ctgtaatggc ggtgaccacg gttatccatt
    25921 tgcaaccaac tttaatccag aaattaatct ccgtgaggtt tctgcgccag gttcttactg
    25981 ggaagatggg aaatgggtcg aagtcgaagc tatgtctatc aagcgtgagt atgatttccc
    26041 tcaagttgga caaaaagata tgtatctcct tcaccatgaa gaaatcgaat cattggccaa
    26101 gaacattcca ggtgtcaaac gcattcgttt ctttatgact tttggtcaat cttacttgac
    26161 gcacatgaaa tgtcttgaaa atgttggact ccttcgtacg gataccatta actttaacgg
    26221 ccaagaaatt gttccaattc aatttttgaa agccttgctt ccagatcctg ctagccttgg
    26281 tccacgtact gtaggtaaaa ccaatattgg atgtatcttt acaggtgtca aagacggtgt
    26341 caaaaagact atctatatct acaatgtctg cgaccatcag gaatgttacg cagaggttgg
    26401 ttcgcaagct atttcttata cgacaggagt tccagccatg attgggacaa aattagtcat
    26461 gaacggaact tggaaacaag ctggagtgta taaccttgag gagttagatc cagatccatt
    26521 catggaagct ttgaatgagt atggtttgcc atgggttgtg gttgaaaatc cacaaatggt
    26581 ggactaatga agttagaaca agtaccaaca ccagcctatg ttattgactt ggccaagtta
    26641 gaagctaatt gccgcattct acaatatgta caagaagagg ccggttgcaa ggtcttgctt
    26701 gcccagaagg catattccct ctacaaaact tatcccttga ttagccagta tctatcaggt
    26761 acgacagcta gtggactcta tgaggccaaa ttggcaaggg aagaatttcc tggtgaagtc
    26821 catgtttttg caccagcttt caaggatgag gacatggagg aattgcttgg gataacagac
    26881 catatcgtct ttaactcaga gagacagttg cgtaagcatg gttcgcgttg tcgagaggct
    26941 ggtgtcagtg ttggtttgcg cctcaaccct cagtgttcaa ctcaaggaga tcacgcgctc
    27001 tatgaccctt gtgcaccagg ttctcgcttt ggagttacta tagacaagat tccgagtgat
    27061 ttgctagatt tggttgacgg acttcatttt catacccttt gcgagcaggg agcagatgat
    27121 ttacaaacaa ctttgaaagc agtagaagaa cagtttggtc cctacttaca tgaggtaaaa
    27181 tggctcaata tgggtggtgg tcatcatatt acaagagaag gttacgatgt ggatttgctg
    27241 atttcagaaa tcaagcgtat ccgaaaaact tacaatcttg aaatctatat cgagcctggt
    27301 gaagccattg cgcttaatgc gggttattta gcaactgagg tattagatat tgtagaaaac
    27361 ggtatggaaa tcttggtttt agacgcctct gcgacctgcc atatgcctga tgtacttgag
    27421 atgccctatc gtccaccttt gagaaatggc tttgaggcac aggaaaaagc ccatacctac
    27481 agactttctt ctaatacctg tctgacgggc gatgtgattg gtgattatag ttttgaaaat
    27541 ccagtccaaa tcggagacag actttatttt caagacatgg ccatttattc ttttgtcaaa
    27601 aataatacct ttaatggtat tggattgcca agtctctatc tcatggacga acagggagac
    27661 tgtagcttac tcaaagcttt tggctatcaa gactttaaag ggagattatc atgatggaca
    27721 gtccaaaaaa attaggctat cacatgccag cagagtacga accccatcat ggtaccctca
    27781 tgatatggcc gactcgacca ggatcatggc cttttcaagg aaaggatgct aaaagagcat
    27841 ttactcagat catcgagact atagcagaag gggaaagagt ctatcttttg gtggagcagg
    27901 cctatctatc tgaagcccaa tcctatcttg gagacaaggt tgtttattta gacattccca
    27961 ccaatgatgc ctgggcgcgt gatactggcc caaccattct cgtcaatgac aaaggtaaga
    28021 aattagccgt ggattgggcc ttcaatgctt ggggaggcac ctatgatggt ctttatcaag
    28081 attatgaaga ggatgaccaa gtagccagtc gttttgctga ggccttggaa aggcctgtct
    28141 atgatgctaa accttttgta ctggaaggag gcgcaatcca tagcgatggt caaggaacta
    28201 ttctcgtaac tgaaagttgc ttgcttagtc ctggtcgcaa tcctaacttg actaaagagg
    28261 agattgaaaa cacattatta gaaagtcttg gtgctgaaaa agttatttgg cttccttatg
    28321 gtatttatca ggatgaaacc aatgaacacg tcgataatgt tgctgccttt gttggtcctg
    28381 ctgaggttgt tttggcttgg acagatgacg aaaatgatcc ccagtatgcc atgtcaaaag
    28441 cagatctcga actcttagaa caggaaacag atgcaaaagg ttgtcacttc accattcata
    28501 aattgcctat ccctgcagtt cgacaagttg tgacagaaga agatttgcca ggctacatct
    28561 atgaagaagg agaagaagag cgatacgcag gtgaacgact agcagcttcc tacgtaaact
    28621 tttatatcgc caacaaggct gtcttggttc cacagtttgc ggatgtaaac gaccaagtgg
    28681 ccttagatat cctcagcaag tgtttcccag accgtaaagt tgtcggaata ccagccagag
    28741 atattctctt aggtggtggc aatatccact gtatcaccca acaaattcca gaataggaga
    28801 aaaagatgag aaatgtaaga gttgcaacca ttcagatgca atgcgctaag gatgtggcaa
    28861 caaatatcca aaccgcagag cgtttagtac gtcaggctgc tgagcaagga gcccaaatta
    28921 ttctcttgcc cgagttgttt gaacatccct atttctgtca ggaacgtcag tatgactact
    28981 accagtatgc ccaatctgta gcggaaaata ctgccattca gcattttaag gtgattgcta
    29041 aggaactaca agttgtttta ccaatcagtt tctatgaaaa agatggtaat gtcttgtata
    29101 actctattgc cgtcattgat gcagatgggg aagtgctggg cgtttatcga aagacccata
    29161 taccagatga ccattattat caagaaaaat tctatttcac gcctggtaac actggtttca
    29221 aggtctggaa tactcgctat gctaagattg gtatcggtat ctgttgggat caatggttcc
    29281 ctgaaacagc gcgctgtctt gcattgaatg gtgctgaatt gctcttttat cctacagcta
    29341 tcggttcaga gccaattttg gatacagata gttgtggtca ctggcaacgt actatgcaag
    29401 ggcacgcagc agcgaatatt gttccagtca tcgcagccaa tcgttatggt ttagaggagg
    29461 ttactcctag tgaggaaaat ggcggacaga gttccagtct tgacttctac ggttcctcct
    29521 ttatgacgga tgaaacagga gctattctag aacgagctga aagacaagaa gaagctgttc
    29581 tgttagctac ttatgaccta gacaagggag caagtgaacg tctaaactgg ggcttgtttc
    29641 gagatagaag accagaaatg tatagacaaa ttacagatta gtgtgggaga aatgagagat
    29701 tcattctgct agactaactt cttattagca actataagat actatggcat ctagtaaatc
    29761 gatttttatg attcgctatt cttgtctatt gattagtccg tattttaaaa tattagcaaa
    29821 aaagcaaata gcagtaactt ctgtctattt gcttttcttt tttatagaat atatttctca
    29881 atagcacgcg caacgccgtc ttcttcgttg cttgaggtaa cggcatccgc aagagatttg
    29941 acataatcgc tggcatttcc cattgcaatc ccaagccctg caaactggag catttcgata
    30001 tcgttattag catcgcccat ggccataatc tctgaggaat caatcttcaa aatctcagct
    30061 agtcgtgaaa gagcagtagc ctttgtcgtt ccaagtggca ttgcttcata aatgacaggc
    30121 tgcgaacgaa ctccactgaa tcgttggcaa agctcttcag caaaacgctg ctcaaaatcg
    30181 tctgtttgtt cttttgttcc taaaaacata ccttggaaca tccggaactt tccactagtc
    30241 gcttcctcaa gagaaatttc agtcaggtct gaaaatacta gtttagcatc attttcaata
    30301 acttgattgg gcttgccacc gagaacaaaa taatgtgact cgtcaaaaag tgtcaactga
    30361 acatcactct tttcagcaag gtcatagagg tattcgatgt cagctggact cagttctttc
    30421 cagtcaacta gaccccaatc actggtttgg tgagttgaac aaccgttgtt aacaataaca
    30481 tattcgttct ggaggtcaag ccccagtttt ttgtagtagg ggaggacacc gaaaaggggg
    30541 cgacccgtac agagaaccag tttgacacct ttttcaatgg ctttgtgaat agcagtaatg
    30601 tgagcttgtg ggatttcctt ggcttcattg aggagggtgc cgtccatatc caaggctagt
    30661 agtttaatca taggtcttcc ttctttatct tttggtatta ttatagcata ttttggataa
    30721 gaaattgata gaaagcttga gactaattga ttttatagtt taagatgttt tgatgacaat
    30781 tcatgatttg aagaggatat ttggcaaaga tatgctatac tatgtttgtc aatgttgcaa
    30841 ctagacaaat taaaaaacca acttaatata atagtttttt tgtaagtagg tatgagtagc
    30901 agattactca actaatctaa acaataacgg aggaaatata tcatgatttt aatgacaaaa
    30961 aatataaatc taacaaatga agaattagag ctgatacaag gtggagcaga tccctatggt
    31021 aaagaaccta atggttatta tccttggaaa atggaaccag tattaactct gctggttcat
    31081 ggattttgtc ccagagacac cgatgattta ggatatattg gaggaggtaa tcatctttgc
    31141 aaaggaagtg ctgcgagatt ttaagtaaaa tttattagta atatgaagaa acaagtggag
    31201 aaagcagaga atttaatatg aaaaaacggg ctattcaaat tttactagca ttgtccttaa
    31261 ttttttacaa atcaacttgg ttttggaggc ttttcaatta tctcgcaaag ccctatctac
    31321 cagcaagtcg tgaatttttt cagattctgc ttttgatgga gagtggagtt cttttattag
    31381 cggtcatcta tctactagtt tttgcaggaa agaaaacttt tcatttcaag tggcagctga
    31441 ggtattttat ctacctttta ctgggttaca tcacttcgta tatgtctgac tttctctttt
    31501 cgtatttcat atccctgtct tcaaatcaaa tttctttgaa tgaaacgata gaaatgatag
    31561 ggagacagga attaccctat tttttgctca tcgtttgctt catcggccct attgctgagg
    31621 aattgattta tcgcggtgtg cttatgacaa cctttttcaa aaactcgcct tggtacggag
    31681 atgtcttgct ttctgctatt attttcggtt atattcatat caattttgct ttaactcctc
    31741 ttgctttttt catttatgct agcggaggtc ttattttggc tctattgtat cgcatgacta
    31801 aaaacatcta ctatccaata ctagttcata ttctcatcaa tatcactgct ttctgggatg
    31861 tttggttact cctattttca ggaagttagc ttactaaaaa atgtcggaat tttccggcat
    31921 tttctttttt cacaaatagt caacgttttt ctttccgata ctgaagtggt gtgtagccac
    31981 ttattttttt gaattgattt tgaaaataag attggcgtga gaaaggcaga tagtgaagat
    32041 agttaagaag aataggatgt tcttttttcc tttttggaaa acttctaaaa tatggtataa
    32101 taaaaagata aagaagttgg gggtagaaga tgaacattca acaattacgc tatgttgtgg
    32161 ctattgccaa tagtggtact tttcgtgaag ctgctgaaaa gatgtatgtt agtcagccga
    32221 gtctgtctat ttctgttcgt gatttggaaa aagagttggg ctttaagatt ttccgtcgga
    32281 ccagctcagg gactttcttg acccgtcgtg ggatggaatt ttatgaaaaa gcgcaagaat
    32341 tggttaaagg atttgatatt tttcaaaatc agtatgccaa tcctgaagaa gaaaaagatg
    32401 aattttccgt tgctagccag cactatgact tcttaccacc aactattacg gccttttcag
    32461 agcgctatcc tgactataag aacttccgta tttttgaatc aactactgtt caaatattag
    32521 atgaagtggc gcaagggcat agtgagattg ggattatcta cctcaacaat caaaataaaa
    32581 aggggattat gcaacgggtt gaaaagttag gtctggaggt catcgaattg attcctttcc
    32641 atacccatat ttatctctgt gagggtcatc ctttagctcg gaaagaggaa ttagtcatgg
    32701 aggatttagc ggatttacca acggttcgtt tcactcaaga gaaagacgag tacctttatt
    32761 attcagagaa ctttgtcgat accagcgcta gctcacagat gtttaatgtg acagaccgtg
    32821 ccaccttgaa tggtattttg gagcggacgg acgcctatgc gacaggttct ggatttttag
    32881 atagtgacag tgttaatggc attacagtta ttcgtctcaa ggataaccta gataaccgca
    32941 tggtctatgt taaacgtgaa gaagtggagc ttagtcaagc tgggactctc ttcgtagaag
    33001 tcatgcaaga atattttgat caaaagagga aatcatgaaa aaaagagcaa tagtggcagt
    33061 cattgtactg cttttaattg ggctggatca gttggtcaaa tcctatatcg tccagcagat
    33121 tccactgggt gaagtgcgct cctggattcc caatttcgtt agcttgacct acctgcaaaa
    33181 tcgaggtgca gccttttcta tcttacaaga tcagcagctg ttattcgctg tcattactct
    33241 ggttgtcgtg ataggtgcca tttggtattt acataaacac atggaggact cattctggat
    33301 ggtcttgggt ttgactctaa taatcgcggg tggtcttgga aactttattg acagggtcag
    33361 tcagggcttt gttgtggata tgttccacct tgactttatc aactttgcaa ttttcaatgt
    33421 ggcagatagc tatctgacgg ttggagtgat tattttattg attgcaatgc taaaagagga
    33481 aataaatgga aattaaaatt gaaactggtg gtctgcgttt ggataaggct ttgtcagatt
    33541 tgtcagaatt atcacgtagt ctcgcgaatg aacaaattaa atcaggccag gttttggtca
    33601 atggtcaagt caagaaagct aaatacacag tccaagaggg tgatgtcgtc acttaccatg
    33661 tgccagaacc agaggtatta gagtatgtgg ctgaggatct tccgctagaa atagtctacc
    33721 aagatgagga tgtggctgtc gttaacaaac ctcagggaat ggttgtgcac ccgagtgctg
    33781 gtcataccag tggaacccta gtaaatgccc tcatgtatca tattaaggac ttgtcgggta
    33841 tcaatggggt tctgcgtcca gggattgttc accgtattga taaggatacg tcaggtcttc
    33901 tcatgattgc taaaaacgat gatgcgcatc tagcacttgc ccaagaactc aaggataaaa
    33961 agtctctccg caaatattgg gcgattgttc atggaaatct gcctaatgat cgtggtgtaa
    34021 ttgaagcgcc gattggccgg agtgaaaaag accgtaagaa acaggctgtg actgctaaag
    34081 ggaagcctgc agtgacgcgt tttcacgtct tggaacgctt tggcgattat agcttagtag
    34141 agttgcaact ggagacaggg cgcactcatc aaatccgtgt ccacatggct tatatcggcc
    34201 atccagtcgc tggtgatgag gtctatggtc ctcgcaagac tttgaaagga catggacaat
    34261 ttcttcatgc caagacttta ggttttactc atccgagaac aggtaagacc ttggaattta
    34321 aagcagatat cccagagatt tttaaggaaa ccttggagag attgagaaag taagaatgaa
    34381 aaagaaatta actagtttag cacttgtagg cgctttttta ggtttgtcat ggtatgggaa
    34441 tgttcaggct caagaaagtt caggaaataa aatccacttt atcaatgttc aagaaggtgg
    34501 cagtgatgcg attattcttg aaagcaatgg acattttgcc atggtggata caggagaaga
    34561 ttatgacttc ccagacggta gtgattctcg ctatccatgg agagaaggaa ttgaaacgtc
    34621 ttataagcat gttctaacag accgtgtctt tcgtcgtttg aaggaattgg gtgtccaaaa
    34681 acttgatttt attttggtga cccataccca cagtgatcat attggaaatg ttgatgaatt
    34741 actgtctacc tatccagttg accgagtcta tcttaagaaa tatagtgata gtcgtattac
    34801 taattctgaa cgtctatggg ataatctgta tggctatgat aaggttttac agactgctgc
    34861 agaaaaaggt gtttcagtta ttcaaaatat cacacaaggg gatgctcatt ttcagtttgg
    34921 ggacatggat attcagctct ataattatga aaatgaaact gattcatcgg gtgaattaaa
    34981 gaaaatttgg gatgacaatt ccaattcctt gattagcgtg gtgaaagtca atggcaagaa
    35041 aatttacctt gggggcgatt tagataatgt tcatggagca gaagacaagt atggtcctct
    35101 cattggaaaa gttgatttga tgaagtttaa tcatcaccat gataccaaca aatcaaatac
    35161 caaggatttc attaaaaatt tgagtccgag tttgattgtt caaacttcgg atagtctacc
    35221 ttggaaaaat ggtgttgata gtgagtatgt taattggctc aaagaacgag gaattgagag
    35281 aatcaacgca gccagcaaag actatgatgc aacagttttt gatattcgaa aagacggttt
    35341 tgtcaatatt tcaacatcct acaagcctat tccaagtttt caagctggtt ggcataagag
    35401 ttcatatgga aactggtggt atcaagcgcc tgattctaca ggagagtatg ttgtcggttg
    35461 gaatgaaatt aagggtgaat ggtattactt taaccaaacg ggtatcttat tacagaatca
    35521 atggaaaaaa tggaacaatc gttggttcta tttgacagac tctggtgttg ctgctaaaaa
    35581 ttggaagaaa atcgctggaa tctggtatta ttttaacaaa gaaaaccaga tggaaattgg
    35641 ttggattcaa gataaagagc agtggtatta tttggatgtt gatggctcta tgaagacagg
    35701 ttggcttcaa tataaggggc aatggtatta ctttgctcca tcaggggaaa tgaaaacggg
    35761 ctgggtaaaa gataaagaag cctggtacta tatggattct actggtatca tgaagacagg
    35821 tgagatagaa gttgctggac atcattacta tctagaagaa tcaggagcta tgaagcaagg
    35881 ctggcttaaa aaggcaaatg attggtattt ctacaagaca gatggttcac gagctatagg
    35941 ttggattaaa gacaaggata aatggtactt cttgaaagaa aatggtcaat tacttgtgaa
    36001 tggtaagaca ccagaaggct atactgtgga ttcaagtggt gcctggttag tggatgttcc
    36061 gatcgagaaa tctgctacaa ttaaaactac aagtcattca gaaataaaag aatccaaaga
    36121 agtagtgaaa aaggatcttg aaaataaaga aacgagtcaa catgaaagtg ttacaaattc
    36181 ttcaactagt caagatttga catcctcaac ttcacaaagc tctgaaacga gtgtaaacaa
    36241 atcggaatca gaacagtagt agaaaagaag gttttagggc cttctttttc ctatcaactc
    36301 ttttctattt cctgttattc atgttataat ggataaatat gaataatcgg agtgagacta
    36361 tgaaatacaa acggattgtc tttaaggtgg gtacttcttc tctgacaaat gaggatggaa
    36421 gtttatcacg tagtaaggta aaggatatta cccagcagtt ggctatgctg cacgaggctg
    36481 gtcatgagtt gattttggtg tcttcaggtg ccattgcggc tggttttgga gccttaggat
    36541 ttaaaaagcg tccgactaaa attgctgata aacaggcttc agcagcggta gggcaggggc
    36601 ttttgttgga agaatataca accaatcttc tcttgcgtca aatcgtttct gcacaaatct
    36661 tgctgaccca agatgacttt gtggataagc gtcgttataa aaatgcccat caggctttgt
    36721 cggttttgct caaccgtggg gcaattccta tcatcaatga gaatgatagt gtcgttattg
    36781 atgaggtcaa ggttggggac aatgacactc taagtgccca agtagcggcg atggtccaag
    36841 cagacctttt ggttctcttg acagatgtgg acggtctcta tactggaaat cctaattcag
    36901 atccaagagc caaacgcttg gagagaatcg agaccatcaa tcgtgagatt attgatatgg
    36961 ctggtggagc tggttcgtca aacggaactg ggggtatgtt aaccaaaatc aaggctgcaa
    37021 ctatcgcgac ggaatcagga gttcctgttt atatctgctc atccttgaaa tcagattcca
    37081 tgattgaggc ggcagaggag accgaggatg gttcttactt tgttgctcaa gagaaggggc
    37141 ttcgtaccca gaaacaatgg cttgccttct atgctcagag tcaaggttct atttgggttg
    37201 ataaaggggc tgcggaagct ctctctcaac atggaaagag tcttctctta tctggtatcg
    37261 ttgaagcaga aggagtcttt tcttacggtg atatcgtgac agtatttgac aaggaaagtg
    37321 gaaaatcact tggaaaagga cgcgtgcaat ttggagcatc tgctttggag gatatgttgc
    37381 gttctcaaaa agccaagggt gtcttgattt accgtgacga ctggatttcc attactcctg
    37441 aaatccaact actttttaca gaattttaga ggtaaactat ggtgagtaga caagaacaat
    37501 ttgaacaggt acaggctgtt aaaaaatcga ttaacacagc tagtgaagaa gtgaaaaacc
    37561 aagtcttgct agccatggct gatcacttag tggctgctac tgaggaaatt ttagcggcta
    37621 atgccctcga tatggcagcg gctaagggga aaatctcaga tgtgatgttg gatcgtcttt
    37681 atttggatgc agatcgtata gaagcgatgg caagaggaat tcgtgaagtg gttgccttac
    37741 cagatccaat cggtgaagtt ttagaaacaa gtcagcttga aaatggtttg gttatcacaa
    37801 aaaaacgtgt agctatgggg gtcatcggta ttatctatga aagccgtcca aatgtgacgt
    37861 ctgatgcggc tgctttgact cttaagagtg gaaatgcggt tgttcttcgt agtggtaagg
    37921 atgcctatca aacaacccat gccattgtca cagccttgaa gaagggcttg gagacgacta
    37981 ctattcatcc aaatgtgatt caactggtgg aggatactag ccgtgaaagt agttatgcta
    38041 tgatgaaggc caagggctat ctagaccttc tcattcctcg tggaggagct ggcttgatta
    38101 atgcagtagt tgagaatgcc attgtgcctg ttatcgagac aggaactggg attgtccatg
    38161 tttatgtcga taaggacgca gatgacgaca aggcactgtc tatcatcaac aatgccaaaa
    38221 ccagtcgtcc ttctgtctgc aatgccatgg aggttctgct ggttcatgaa gacaaggcag
    38281 caagcttcct tcctcgcttg gagcaagtgc tggttgcaga tcgaaaagaa gctgggttgg
    38341 aaccaattca attccgccta gatagcaaag caagccagtt tgtttcaggt caagctgctc
    38401 aagcacaaga ctttgatacc gagtttttag actatattct agctgttaag gttgtgagca
    38461 gtttagaaga agcggttgcg catattgaat cccacagtac ccatcattcg gatgctattg
    38521 tgacggaaaa tgctgaagct gcagcatact ttacagatca agtggactct gcagcggtgt
    38581 atgttaatgc ctcaactcgt ttcacagatg gaggacaatt tggtcttggt tgtgaaatgg
    38641 ggatttctac tcagaaattg cacgcgcgtg gtcccatggg cttgaaggaa ttgactagct
    38701 acaagtatgt ggtcactggt gatggacaga taagggagta agagatgaag attggattta
    38761 tcggtttggg gaatatgggt gctagtttag ccaagtctgt cttgcaggct agaccgtcag
    38821 atgagattct ccttgccaat cgtagtcaag tcaaggtgga tgctttcatc gctaacttcg
    38881 gtggtcagac ttctaacaat gaagaaatat tcgaagaagc agatgtgatt tttctaggtg
    38941 ttaagcctgc tcagttttct gaactgcttt ctcaatacca gaccatcctt gaaaaaagag
    39001 aaagtcttct tttgatttcg atggcagctg gattgacctt agaaaaacta gcaagtctta
    39061 tcccaagcca acatcgaatt attcgtatga tgcctaatac ccctgcttct atcgggcaag
    39121 gagtgattag ttatgccttg tctcctaatt gcagggctga ggacagtgag atcttttgtc
    39181 agcttttagc caaggctggt ctcttggttg aattaggaga aagcttaatc aatgcagcga
    39241 caggtcttgc aggttgtgga ccagcctttg tctatctctt tattgaggcc ttggcagatg
    39301 caggtgttca gacgggatta ccacgagaaa cagccttgaa aatggcagcc caaactgtgg
    39361 taggagctgg gcaattggtc ctagaaagcc agcaacatcc tggagttttg aaagatcaag
    39421 tttgtagtcc aggcggttcg actattgttg gcgtagcaag cttagaagcg catgcttttc
    39481 gaggcatagt catggaggca gttcatcaag cctataaacg aacacaagaa ctaggtaaat
    39541 aagaggtagt tttatctgcc tcttttatgg tggttgatac tcttcgaaaa tcaaattcaa
    39601 actgcgtcag cttcaccttg ccgtactcaa gtacagcctg cggctagctt cctagtttgc
    39661 tctttgattt tcattgagta tgaaatgaga agacaaaaag attgtcacaa acccctattt
    39721 ttttgataga atagaagtag taaaaaagaa atgagttaga catgtcaaaa ggatttttag
    39781 tctctcttga gggaccagag ggagcaggca agaccagtgt tttagaggct ctgctaccaa
    39841 ttttagagga aaaaggagta gaggtgttga cgacccgtga acctggcgga gtcttgattg
    39901 gggagaagat tcgggaagtg attttggatc caagtcatac tcagatggat gctaaaacag
    39961 agctacttct ctatattgcc agtcgcagac agcatttggt ggaaaaagtt cttccagccc
    40021 ttgaagctgg caagttggtc atcatggatc gttttatcga tagttctgtt gcctatcagg
    40081 gatttggtcg tggcttagat attgaagcca ttgactggct caatcagttt gcgacagatg
    40141 gcctcaaacc cgatttgaca ctctattttg acatcgaggt ggaagaaggg ctggctcgta
    40201 ttgctgctaa tagtgaccgc gaggttaatc gtttggattt ggaagggttg gacttgcata
    40261 aaaaagttcg tcaaggctac ctttctcttc tggataaaga gggaaatcgc attgtcaaga
    40321 ttgatgctag tctccctttg gagcaagttg tggaaactac caaggctgtc ttgtttgacg
    40381 gaatgggctt ggccaaatga aacaagatca actaaaggct tggcaaccag ctcagtttga
    40441 ccgttttgtc cgtatcttag aacaagacca gctcaatcac gcctatctct tttcaggttt
    40501 ctttggaagc ttggaaatag cgcaattttt agctaagagc ctcttttgta cggataaagt
    40561 tggcgtctta ccatgtgaga aatgccgaag ttgcaagctg attgaacagg gagaatttcc
    40621 cgatgtcacc ttgattaagc cagtcaatca ggtcatcaag acagaacgca ttagggaatt
    40681 ggtgggacag ttttctcaag cagggattga aagccagcaa caggtcttta ttatcgagca
    40741 agcggataaa atgcatccca acgcagccaa ttctctgctc aaggtcatcg aagaacccca
    40801 gagtgaagtt tatattttct tcttgactag cgatgaggaa aagatgttac cgacaatccg
    40861 aagtcggact cagatcttcc actttaaaaa gcaagaagaa aaacttatct tactcttaga
    40921 acaaatggga cttgttaaga aaaaagcgac tcttttagct aagtttagtc aatcgcgagc
    40981 tgaagcagaa aagttggcta atcaggcaag tttttggacc ttggtcgatg aaagtgaacg
    41041 cctgctgact tggttagtag ctaagaaaaa agaaagttat ctacaggttg ccaaattagc
    41101 caacttggca gatgataagg aaaaacagga tcaggtttta cggattcttg aagttctctg
    41161 cgggcaggac ctcttgcagg taagagtaag agtgattcta caagatttac tagaagctag
    41221 aaaaatgtgg caagctaatg tcagctttca aaatgccatg gaatatctgg tcttgaaaga
    41281 aatataaact caaaaatgaa tgataaagaa aggaaagggc tgttttatgg acaaaaaaga
    41341 attatttgac gcgctggatg atttttccca acaattattg gtaaccttag ccgatgtgga
    41401 agccatcaag aaaaatctca agagcttggt agaagaaaat acagctcttc gcttggaaaa
    41461 tagtaagttg cgagaacgct tgggtgaggt ggaagcagac gctcccgtca aggccaaaca
    41521 tgttcgcgaa agtgtccgtc gtatttaccg tgatggattt cacgtatgta atgattttta
    41581 tggacaacgt cgagagcagg acgaagaatg tatgttttgt gatgagttgt tatacaggga
    41641 gtaggcatgc aaattcaaaa aagttttaag gggcagtctc cctatggcaa gctgtatcta
    41701 gtggcaacgc cgattggcaa tctagatgat atgacttttc gtgctatcca gaccttgaaa
    41761 gaagtggact ggattgctgc tgaggatacg cgcaatacag gtcttttgct caagcatttt
    41821 gacatttcca ccaagcagat cagttttcat gagcacaatg ccaaggaaaa aattcctgat
    41881 ttgattggtt tcttgaaagc agggcaaagt attgctcagg tctctgatgc cggtttgcct
    41941 agcatttcag accctggtca tgatttggtt aaggcagcta ttgaggaaga aattgcagtt
    42001 gttacagttc caggtgcctc tgcaggaatt tccgccttga ttgccagtgg tttagcgccg
    42061 cagccacata tcttttacgg ctttttaccg agaaaatcag gccagcaaaa gcaatttttc
    42121 gactcgaaaa aagactatcc tgaaactcag attttttatg aatcgcccca tcgtgtggca
    42181 gatacattgg aaaatatgct agaagtctac ggtgaccgct ccgttgtctt ggtcagggaa
    42241 ttgaccaaaa tctatgaaga ataccaaaga ggtacaattt ctgaattgct ggaaagcatc
    42301 tctgaaacgt ctctcaaggg tgaatgtctt ctgattgttg aaggtgccag tcaggatgtg
    42361 gaagaaaaag acgaggaaga cttgttctta gaaatccaag ctcgtatcca gcaaggtatg
    42421 aagaaaaatc aagctattaa ggaagtagct aagatttacc agtggaataa gagtcaactc
    42481 tacgctgcct accacgactg ggaagaaaaa caataaaggg agacaggatg taataattct
    42541 gtctgtttct gtttaactta attagtgatg ataatataaa gatgtatcac ttggtataga
    42601 agctttggta ttaagttttt tattaagccc atacggaata ccgatggttg gagcagcagt
    42661 tatagcgttc ttagaaggta taaatagaaa aataaggtca ttttaaatca aaggattgat
    42721 aaatcagaaa gaaggtgatt ttttgcgaac atacgaaaat aaagaagaac taaaagctga
    42781 gatagagaaa acatttgaga aatatatttt agaatttgat aatattccaa aaaatttaaa
    42841 agataagaga gctgatgaag ttgacagaac tccagcagaa aaccttgctt atcaggttgg
    42901 ttggaccaac ttggttctta aatgggaaga agatgaaaga aaggggcttc aagtaaaaac
    42961 accatcggat aaatttaaat ggaatcaact tggtgaatta tatcagtggt tcacagatac
    43021 ctacgctcat ttatctctgc aagagttgaa agcaaaatta aatgaaaata ttaattctat
    43081 ctctgcaatg attgattcgt tgagtgagga agaattattt gaaccgcata tgagaaagtg
    43141 ggctgatgaa gcgactaaaa cagcgacttg ggaagtgtat aagtttattc atgtaaatac
    43201 ggttgcacct tttggaactt tcagaactaa aatcagaaaa tggaagaaga tagtattata
    43261 aattatattt ttaactttaa aaaatttcat aaaaatggtt accaaaggcg atagaagaaa
    43321 aactatcgtc tttttctttg caaattttta agaagggagg tgatcttgca tggactttga
    43381 atatttttat aacagagaag cggaaagatt taacttctta aaagtaccgg agatattagt
    43441 tgatagagaa gaatttcggg gcttatcagc agaagcaatt atcctttatt ccatacttct
    43501 taaatagaca ggaatgtcat ttaagaataa ctggatagac aaggaaggca gagtatttat
    43561 ctattttact gtcgaagaaa ttatgaaaag aagaaatatc tcaaagccaa ctgccataaa
    43621 aacattagat gagcttgatg taaaaaaagg aataggactg atcgaaagag taaggcttgg
    43681 acttggtaag ccgaacatca tttatgttaa agactttatg aatatatttc aggtaaaaga
    43741 aaatgactta cagaagtcaa aaaacttaac ttcagaagta aaagatttta acctcagaag
    43801 taaagaaaat gaacttcaag aggttaagaa ccttgactct aactatatag agaataataa
    43861 gagtaagtat agtaagagag aatatagttt tggtgaaaac ggacttggaa catttcaaaa
    43921 tgtgttttta gctgctgaag atatatcgga tttacaaatc ataatgaact cacagcttga
    43981 gaattacatt agacttcctg caaaactaga atcctagttc atgattgata ataccagcac
    44041 tcaaattcat tcgtaatccg aagcgtttac gatgatttcg ataggttgtt gaaaacattt
    44101 taaacgtttt tactttggca aagatattct caaccttgct tctctcctta gatagcgcat
    44161 gattacaggc tttatcttca actgttagcg gcttgagttt gctggattta cgtggagttt
    44221 gtacttgagg atatatcttc atgagccctt gataaccact gtcagccaag atttttccag
    44281 cttgtccgat atttctgcga ctcattttga acaacttcat atcgtggcaa tagttcacag
    44341 tgatatccaa agaaacaatt ctcccttgac ttgtgacaat cgcttgagcc ttcatagcgt
    44401 gaaatttctt tttaccagaa taattggcta gttgattttt ttaggacgat ttatttttac
    44461 ctctgttgca tccacaatca ccgtatcctc agcactaaga tgagtttttg aaatcgtaac
    44521 accactttga acaagagtta cttcaaccca ttggctccga cggattaagt tgctttcgtg
    44581 aataccaaga tcagccgcaa tttcttcata agtgcggtct tctcacacat attgaagagt
    44641 ggccataaga aggtcttcta ggcttaattt aggttttcgt ccaccttttg cgtgtttaag
    44701 ttgataagct gtttttaata cagctaacat ctcttcaaaa gtggtacgct gaacaccaac
    44761 aagacgctta aatcgtgcat cagttagttg tttacttgct tcatcattca tagaactact
    44821 ataccatatt ttgtttcgca ggaagtctat tggaaagtaa gaaatattga agctgaggct
    44881 attagaagaa attgtgagcg tggtgctatt ttttcaggta aaataaaata tcacgaagat
    44941 tcacagttta aaggagatca ctatgttgaa tgttatgctg ttttagataa tacggttata
    45001 gcaagagata gaataacagt ccctatcgat ccgttatgtg gaaaagattt tatagagtag
    45061 catataattg attcttaact ggaatactca ctatctcttt acatcaagaa aatgactaaa
    45121 cagggaagtt tgccttcttc cctttttttg ttatactagt agaagaaaaa atagaaagat
    45181 ttgtgggagt gaaaaagtcc tgtggacttt ttcagcctga gccaagaaac tcgaaagctt
    45241 gtaagtctga ttggcttttt caatgtgaat cttaacttca cactcccaaa gaggtattag
    45301 tgtcgtgtct caatcttata tcaatgttat cggtgctggt ttggcaggtt ctgaagcagc
    45361 ttaccaaatc gcagagcgtg gtattccagt taaactatat gaaatgcgtg gtgtcaagtc
    45421 tacaccccag cataaaacag acaattttgc tgagttggtt tgttccaatt ctttgcgtgg
    45481 ggatgctttg acaaatgcag ttggtcttct caaggaagaa atgcgtcgct tgggttctgt
    45541 tatcttggaa tctgctgagg ctacacgtgt tcctgccggt ggagcccttg cggtggatcg
    45601 tgatggtttc tctcaaatgg tgaccgaaaa agttgccaac caccccttga ttgaagtggt
    45661 tcgtgatgaa attacagaat tgccgacaga tgttattacg gttatcgcta ctggtccttt
    45721 gacaagtgat gccttggctg aaaagattca tgcccttaat gacggtgatg gtttttattt
    45781 ctacgatgcg gcagcgccta ttatcgatgt caacactatc gatatgagca aggtctacct
    45841 caaatcacgt tatgataagg gagaagcggc ctacctcaat gcccctatga ccaagcaaga
    45901 atttatggat ttccatgaag ctttggtcaa tgcagaagaa gcaccgctta attcttttga
    45961 aaaagaaaag tactttgaag gatgtatgcc tatcgaagtt atggccaaac gtggcattaa
    46021 aactatgctt tatggcccta tgaagccagt cggtcttgag tatccggacg actacacagg
    46081 acctcgtgat ggagaattta aaacacctta tgcggttgtg caacttcgtc aggataatgc
    46141 agctggtagc ctctacaata tcgttggttt ccagacccac ctcaaatggg gagaacaaaa
    46201 gcgtgtcttc caaatgattc cgggtcttga aaatgcggag tttgtccgtt atggtgtcat
    46261 gcatcgcaat tcttacatgg attcaccaaa tcttcttgag cagacttacc gttccaagaa
    46321 acaaccaaat ctcttctttg ctggtcagat gacgggggtg gaaggctatg ttgagtcggc
    46381 ggcttcgggc ttagttgcgg gaattaacgc agctcgtctc ttcaaggaag aaagcgaggt
    46441 tattttcccc gagacgacag cgattggaag cttagctcat tacattaccc atgccgacag
    46501 caaacatttc caaccaatga atgtcaattt tgggatcatc aaggagttgg aaggcgagcg
    46561 tatccgtgat aagaaggctc gttatgaaaa aattgcagag cgtgcccttg ccgacttaga
    46621 ggaatttttg actgtctaat ttttttgaaa gaattgctca tgatactata aaaatcttag
    46681 aaattgtgat aaaataggta ggatgaaaga aggagagtga aaatggcgaa tcccaagtat
    46741 aaacgtattt taatcaagtt atcaggtgaa gcccttgccg gtgaacgtgg cgtagggatt
    46801 gatatccaaa cagttcaaac aatcgcaaaa gagattcaag aagttcatag cttaggtatc
    46861 gaaattgcgc ttgttattgg tggaggaaat ctctggcgtg gagaacctgc agcagaagca
    46921 ggtatggacc gtgttcaggc agattacact ggaatgcttg ggactgttat gaatgctctt
    46981 gtgatggcag attcattgca acaagttggg gttgatacgc gtgtacaaac agctattgct
    47041 atgcaacaag tggcagagcc ttatgtccgt ggacgtgccc ttcgtcacct tgaaaaaggc
    47101 cgtatcgtta tctttggtgc tggaattggt tcaccatact tctcgacaga tacaacagcg
    47161 gcccttcgtg cagctgaaat cgaagcagat gccatcctca tggctaaaaa tggcgtcgat
    47221 ggtgtgtaca atgccgatcc taagaaggac aagacagccg ttaagtttga agaattgacc
    47281 caccgtgatg ttatcaacaa aggtcttcgt atcatggact caacagcctc aaccctctca
    47341 atggacaacg acattgactt ggttgtcttc aacatgaacc aatcaggcaa catcaaacgt
    47401 gtcgtatttg gtgaaaatat cggaacaaca gtttcaaata atatcgaaga aaaggaataa
    47461 gaaagaatat ggctaacgta attattgaaa aagctaaaga gagaatgacc cagtctcacc
    47521 aatcacttgc tcgtgaattt ggtggtatcc gtgctggtcg tgccaatgca agcttgcttg
    47581 accgtgtaca tgtagaatac tatggagtcg aaactcctct taaccaaatc gcttcaatta
    47641 cgattccaga agcgcgtgtt ttgttggtaa caccatttga caagtcttca ttgaaagaca
    47701 tcgaacgtgc cttgaacgct tctgatcttg gtatcacacc ggctaatgac ggttctgtga
    47761 ttcgcttggt tatcccagct cttacagaag aaactcgtcg tgaccttgct aaagaagtga
    47821 agaaggtcgg cgaaaatgct aaagtggctg tccgcaatat ccgtcgcgat gctatggacg
    47881 aagctaagaa acaagaaaaa gcaaaagaaa tcactgaaga cgaattgaag actcttgaaa
    47941 aagatattca aaaagtaaca gacgatgctg ttaaacacat cgacgatatg actgccaaca
    48001 aagagaaaga acttttggaa gtctaaaaat aaacagaaaa actcagttgg cattgctggc
    48061 tgagttttat tcgaaagaag gaaatatgaa tacaaatctt gcaagtttta tcgttggact
    48121 gatcatcgat gaaaacgacc gtttttactt tgtgcaaaag gatggtcaaa cctatgctct
    48181 tgctaaggaa gaaggccaac atacagtagg ggatacggtc aaaggttttg catacacgga
    48241 tatgaagcaa aaactccgcc tgacaacctt agaagtgact gccactcagg accaatttgg
    48301 ttggggacgt gtcacagagg ttcgtaagga cttgggtgtc tttgtggata caggccttcc
    48361 tgacaaggaa atcgttgtgt cactcgatat tctccctgag ctcaaggaac tctggcctaa
    48421 gaagggcgac caactctaca tccgtcttga agtggataag aaagaccgta tctggggtct
    48481 cttggcttat caagaagact tccaacgtct tgctcgtcct gcctacaaca acatgcagaa
    48541 ccaaaactgg ccagccattg tttaccgtct caagctatca ggaacttttg tttacctacc
    48601 agaaaataat atgcttggtt ttattcatcc tagcgagcgt tacgcagagc cacgtttggg
    48661 gcaagtatta gatgcgcgcg ttattggttt ccgtgaagtg gaccgcactt tgaacctctc
    48721 cctcaaacca cgctcctttg aaatgttgga aaacgatgct cagatgattt tgacttattt
    48781 ggaaagcaat ggcggtttca tgaccttaaa tgacaagtca tctccagacg acatcaaggc
    48841 aacctttggc atttctaaag gtcagttcaa gaaagcttta ggtggtctta tgaaggctgg
    48901 taaaatcaag caggaccagt ttggtacaga gttgatttag ggaggcttat gagaaaatca
    48961 ttttacactt ggctcatgac cgagcgcaat cctaaaagta acagtcccaa agcaattttg
    49021 gcagacctcg cttttgaaga gtcagccttt ccaaaacata cagatgattt tgatgaggtc
    49081 agtcgctttt tggaggagca tgccagtttc tcttttaacc taggagattt tgacagcatt
    49141 tggcaggaat atctagaaca ctagcattta ttcattgggt ttgggctagt aatttctcca
    49201 tccctctgct ataataaaaa gaaataaaag gattagagag gttctttatt tgaaggaaca
    49261 ttcaatagac attcaactga gtcatccaga tgacctgttt catctttttg gttccaatga
    49321 acgccatctt cgtttgatgg aagaagagct tgatgttgtg attcatgctc gtacggagat
    49381 tgtccaggtt ttgggagaag agtctgcctg tgaggaagcc cgtcaagtta ttcaggcttt
    49441 gatggtcttg gtaaatcgtg ggatgaccgt tggtacgcca gatgtagtca ctgcgattag
    49501 catggtcaaa aatgatgaaa ttgacaagtt tgtcgccctt tacgaagaag aaattatcaa
    49561 agataatact gggaaaccta tccgtgtcaa aaccctaggg caaaagcttt atgtggacag
    49621 tgtcaaacag catgatgtga cctttggaat tgggccagca ggtacaggga agaccttcct
    49681 tgcagtgacc ttggcagtga ctgcccttaa acgtgggcaa gtcaagcgaa ttatcctaac
    49741 tcgtccagcg gtggaagcgg gagagagtct tggatttctt ccgggtgatc ttaaggagaa
    49801 ggtggatcct taccttcgtc ctgtttacga tgccttgtat caaattcttg ggaaagacca
    49861 aacgactcgt ctcatggagc gtgaaattat cgaaattgcg ccccttgcct atatgcgtgg
    49921 tcggaccttg gatgatgcct ttgtcattct cgatgaggcg caaaatacga ccatcatgca
    49981 gatgaagatg ttcttgactc gtttaggttt tcattctaag atgattgtca atggagatat
    50041 tagtcagatt gatctgccac gtaatgtcaa gtccggtttg attgatgccc aagaaaagct
    50101 caagaacatt taccaaattg actttgttca tttttcagct aaggatgtgg ttcgccatcc
    50161 agttgtcgct cagattatcc gagcttatga accagctcca gttaaaaaag aagagagtga
    50221 agaattagag tctcaacctc tgtctgaagg atagttcttt tgtacttaaa gttatctcaa
    50281 tgctaagtat aaatttgctt tttaaatgtt ttattcaata tccttatatt agattagaaa
    50341 cgtttcaagc ggaggaaacg tatggaatca atatttgtga aatttgccca gtatccgtct
    50401 atagaaacgg agcgtttatt gcttagacct gtaactttgg atgatgcgga agcaatgttt
    50461 gactatgcct cggacaaggg taatacacgt tacacttttc caaccaatca aagcttggaa
    50521 gaaaccaaga ataacattgc tcagttctat ttggctaatc ccttgggacg ttggggaata
    50581 gaactaaaaa gcaatggtca gtttattgga accattgact tgcacaagat tgattctgtt
    50641 cttaagaagg cagctattgg ctacattatc aataaaaagt attggaatca aggattaacg
    50701 acagaagcca atcgtgctgt gattgagcta gcttttgaga agatagggat gaataagttg
    50761 actgcccttc acgataagga caatcctgcg tcaggaaagg tcatggagaa atcaggcatg
    50821 cgtttttccc atgcagaacc atatgcttgt atggaccagc atgaaaaaga tcgaatcgtt
    50881 acaagagttc attatgtctt gaccaaggaa gactattttg caaataaata agcagttgaa
    50941 aagaaatttt tcgactgttt tttcttcctc ttacgaataa tctaagagag gagaaaatat
    51001 ggaagcaatt atcgagaaaa tcaaagagta taaaatcatc gtcatctgta ctggtctggg
    51061 cttgcttgta ggcggatttt tcctgctaaa gccagctcca caaacacctg taaaggaaac
    51121 gaatttgcag gctgaagttg cagctgtttc caaggatttg gtatccgaaa aggaagtgaa
    51181 caaggaagag aaggaagaac cagttgaaca agatctaatc acagtagatg tcaaaggtgc
    51241 tgtcaaatcg ccagggattt atgacttgcc tgtaggtagt cgagtcaatg atgctgttca
    51301 gaaggctggt ggcttgacag agcaagcaga cagcaagtcg ctcaatctag ctcagaaagt
    51361 tagtgatgag gctctggttt acgttcctac taagggagaa gaagcagtta gtcaacagac
    51421 tggttcgggg acagcttctt caataagcaa ggaaaagaag gtcaatctca acaaggccag
    51481 tctggaagaa ctcaagcagg tcaagggact gggaggaaaa cgagctcagg acattatcga
    51541 ccatcgtgag gcaaatggca agttcaagtc agtagacgag ctcaagaagg tctctggcat
    51601 tggtggcaaa acaatagaaa agcttaaaga ctatgttaca gtggattaag aatttctcta
    51661 ttcccctaat ttacctgagt tttctattac tttggcttta ttacgctatt ttctcagcat
    51721 cttatcttgc tttgttgggc tttgtttttc tgctagtctg tctctttatc caatttccgt
    51781 ggaaatctgc tggtaaagtt ctaataattt gcggaatctt tggattttgg tttgtttttc
    51841 aaaattggca acagagtcaa gcgagtcaaa atctggcgga ttctgttgaa agggtacgga
    51901 ttttgcctga tactattaag gttaatggtg atagtctatc ctttcgtggc aagtctaacg
    51961 gtcgtgcttt ccaagtctat tataaactcc agtccgagga ggagaaagaa gcctttcaag
    52021 ctttaaccga cctgcatgag ataggactag aagggaagct ttcggagcca gaagggcaga
    52081 gaaattttgg tggctttaat taccaagcct atctgaagac tcagggaatt taccagactc
    52141 tcaatatcaa aacaatccag tcacttcaaa agattggcag ttgggatata ggagaaaact
    52201 tgtccagttt acgtcgaaag gctgtggttt ggattaagac gcactttcca gaccctatgc
    52261 gcaattacat gacaggactc ttgctgggac atctggacac cgactttgag gagatgaatg
    52321 agctttattc cagtctagga attatccacc tctttgccct atctggtatg caggtaggtt
    52381 ttttcatgaa tggatttaag aaacttctct tgcgattggg cttgacccaa gaaaagttga
    52441 aatggctgac ttatcccttt tcccttatct atgcgggact aactggattt tcagcatcgg
    52501 ttattcgcag tctcttgcaa aagctactgg ctcaacatgg ggttaagggc ttggataatt
    52561 ttgccttgac ggtgcttgtc ctctttattg tcatgccaaa ctttttcttg acagcaggag
    52621 gagtcttgtc ctgcgcttat gcttttatcc tgaccatgac cagcaaagaa ggggaggggc
    52681 tcaaggctgt tactagtgaa agtctagtca tctccttggg catattgccc attctatcct
    52741 tctattttgc ggaatttcaa ccttggtcta tccttttgac ctttgtcttt tcctttctat
    52801 ttgacttgac cttcttaccg ctcttgtcta ttttatttgt cctttccttt ctctatccag
    52861 tcattcagct gaactttatc tttgaatggt tggagggcat tattcgcttg gtgtcacagg
    52921 tgacaagtag acctctggtc tttggacaac ccaatgcatg gtttttaatc ctattgttaa
    52981 tttccttggc tttggtctat gatttgagaa aaaacattaa aaagctaacg gtattgtgct
    53041 tattgattac agggctcttt ctcctgacca agcatccact ggaaaatgaa atcaccatgc
    53101 tggatgtggg gcaaggagaa agtattttcc tacgggatgt aactgggaaa accattctca
    53161 tagatgtagg tggtaaggca gaatcttata agaaaatcga aaaatggcaa gaaaagatga
    53221 cgaccagcaa tgcccagcga accttgattc cctatctcaa aagtcgagga gtagctaaga
    53281 ttgaccagct aattttgact aacacggaca aggagaatgt tggagatttg tcagaggtga
    53341 ccaaggcttt ccatgtaggg gagattctag tatcaaaaga cagtctgaaa cagaaggaat
    53401 ttgtggcaga actacagacg actcaaacaa aggtgcgtag tatgacagta ggggagaact
    53461 tgcccatttt tggaagtcag ttagaagttc tatctccaag gaaaatggga gatggaggac
    53521 acgatgacac cctagttctg tatgggaaat tcttggataa gcaatttctc ttcacgggaa
    53581 atttggagga gaaaggagag aaggacttgc tgaagcacta tccagacttg aaagtaaatg
    53641 ttttgaaagc tagccaacat ggcaataaaa aatcatcaag tccagccttt ctagaaaaac
    53701 tcaaaccaga gcttactctt atctcagttg gaaagagcaa tcgaatgaaa ctcccccatc
    53761 aggaaacatt gacacgactg gaaggtatca atagcaaagt ttatcgaact gaccagcaag
    53821 gagctatacg ttttaagggg ttggatagtt ggaaaatcga aagtgttcga taggaaggat
    53881 aaatgttgta gattagtgaa ataaactaaa aatttgttgc ataataatga taaaaatggt
    53941 ataatgaaaa cgtattcaat attgaggata taaaatcatt aaaaatcagc aaaagttgtt
    54001 ttattagtta gtttataatc tattggtctt cttcagtcca gtgtatctgc tgtgacagcc
    54061 actaaaagtt acaagtatga ttggaatacg gttttggaat atagtaccaa ctatcacgac
    54121 catcagtatg cttggattcc ggaatggtct cgttattaca gctattctga gtataaagtt
    54181 ggcggaggct ggaactacgc tcgttatgag gtcataaact actatactgg aggttattaa
    54241 tccttaaaga gtgagaaaag gagggctgga tatgttaaat cttactcatg ttaccttaaa
    54301 aacgcgacaa gtcatcttgc aagatgcgga ttttaccttt aaaaagggta ggatttatgg
    54361 ccttcttgct atcaatggct cgggaaagac gacactattc cgagctatga gcaagttgct
    54421 tccccttagt agtggacaca tcgcagttcc tccttctttg ttttattatg agagcgttga
    54481 atggctggat ggaaacttaa gtgggatgga ctaccttcgt ctcataaaaa acatctggaa
    54541 gtcagaccta aacttgagag atgaaatcgc ctactgggaa atggctgact atatcagtct
    54601 tcccatccgc aagtattcct tagggatgaa gcaacgcttg gtgattgcta tgtattttct
    54661 cagtcaggcc aaatgctggc tcatggatga gattacaaat ggcttagacg agtattatcg
    54721 acagaagttt tttgataggc tagcacaaat cgatagacaa gaacagctgg ttcttttaag
    54781 ttcccactat aaggaagagt tggttgatat ctgcgataga gtagtaacca ttcatcaggg
    54841 gcagatagaa gaggtttagt ttatgaaaga tgttagtcta tttttattga aaaaagtttt
    54901 caaaagtcgt ttaaactgga ttatcttact tttatttgca tctgtactcg gtgttacctt
    54961 ttatttaaat agtcagactg caaactcaca cagcttggag agcaggttgg aaagtcgcat
    55021 tgcagccaac gagagggcta tcaatgaaaa tgaagagaaa ctctcccaaa tgtctgatac
    55081 cagctcggag gaataccagt ttgctaaaaa taatttagac gtgcaaaaaa atcttttgac
    55141 gcgaaagaca gaaattctaa ctttactaaa agaagggcgc tggaaagaag cctactatct
    55201 gcagtggcaa gatgaagaga agaattatga atttgtatca aatgacccga ctgctagctc
    55261 tggcttaaaa atgggggttg accgcgaacg gaagatttac caagccctgt atcccttgaa
    55321 cataaaagca catactttgg agtttccgac ccacgggatt gatcagattg tctggatttt
    55381 agaggttatc atcccaagct tgtttgtggt tgcgattatt tttatgctaa cacaactatt
    55441 tgcagaaaga tatcaaaatc atctggacac agctcactta tatcctgttt caaaagtgac
    55501 atttgcaata tcctctcttg gagttggagt gggatatgta actgtgctgt ttatcggaat
    55561 ctgtggcttt tcttttctag tgggaagtct gataagtggt tttggacagt tagattatcc
    55621 ctacccaatt tatagcttag tgaatcaaga agtaactatt gggaaaatac aagatgtatt
    55681 atttcctggc ttgctcttag ctttcttagc ctttatcgtc attgtggaag ttgtgtactt
    55741 gattgcttac tttttcaagc aaaaaatgcc tgtcctcttt ctttcactca ttgggattgt
    55801 tggtttattg tttggcatcc aaaccattca gcctcttcaa aggattgcac atctgattcc
    55861 ctttacttac ttgcgttcag tggagatttt atctggaaga ttacctaagc aaattgataa
    55921 tgtcaatcta aattggagca tggggatggt cttgcttcct tgcctgatta tccttttgtt
    55981 agtggggatt ctatttatcg aaagctgggg aagttcacgt aaaaaggaag tttttaaagt
    56041 ttagttttcc tatagggaag ctaaagaaaa actaacatag ggagagaatc aacttggttc
    56101 tctctttttg attcgaaaac caaaccaaaa tacaaacaca aacttttcaa aaaataactt
    56161 tttatcttga caagagctag aaaacttggt atcatataaa agttgagaaa agcagaagtg
    56221 agagcttctc gccttgtgac attaagttgc ctggccctac ggatgaaaag tttcgaagaa
    56281 acgctatcat aacgtgcggg cttgtatatt tacaagtccg ctattgtttt tctctaataa
    56341 aacaaaagag gtgaaaacca tagcaaagca agacttattc atcaatgatg agattcgtgt
    56401 acgtgaagtt cgcttgattg gtcttgaagg agaacagcta ggtatcaagc cactcagtga
    56461 agcgcaagct ttggctgata acgctaatgt tgacctagta ttgattcaac cccaagccaa
    56521 accgcctgtt gcaaaaatta tggactacgg taagttcaaa tttgagtacc agaagaagca
    56581 aaaagaacaa cgtaaaaaac aaagcgttgt tactgtgaaa gaagttcgtc taagtccaac
    56641 aattgacaag ggtgactttg atacaaaact tcgtaatgca cgtaagttcc ttgaaaaagg
    56701 aaataaagtt aaggtatcta ttcgctttaa gggtcgtatg attacccata aagagattgg
    56761 tgcaaaagtt ttagccgagt ttgctgaagc aactcaagat attgccatca tcgaacaacg
    56821 tgctaaaatg gatggacgtc aaatgttcat gcaattggcg ccagcgactg acaaaaaata
    56881 attgtcagaa agttaaataa aggagaaaaa atcatgccaa aacaaaaaac acaccgcgca
    56941 tcagctaaac gtttcaaacg tacaggttct ggtggactta aacgtttccg tgcttacact
    57001 tctcaccgtt tccacggaaa aactaagaaa caacgtcgtc atcttcgtaa agcatctatg
    57061 gtgcattcag gagattacaa acgtatcaaa gcaatgctta ctcgcttgaa ataatagctt
    57121 gactgtaagt aaacaattct aggaaatatt tggaggaaat aaaacatggc acgtgttaaa
    57181 ggtggcgttg tatcacgcaa acgtcgtaaa cgtattctta aattagcaaa aggttactat
    57241 ggagctaaac acatcttgtt ccgtactgca aaagaacaag taatgaactc ttactactat
    57301 gcataccgtg accgtcgtca gaaaaaacgt gacttccgca aattgtggat tactcgtatc
    57361 aatgcggcag ctcgtatgaa cggactttca tactcacaat tgatgcatgg tttgaaattg
    57421 gctgagattg aagttaaccg taaaatgctt gctgacttgg ctgttaacga tgcagtagct
    57481 ttcacagctc ttgcagatgc agctaaagca aaacttggta aataataact gatgagactg
    57541 gaacaggatt gtccagtctt tttaaaaata aaggagaaag atatggcttc aaaaatgcta
    57601 cacacttgct tacgagtaga aaatcttgaa aaatcaatcg cattttatca agatgctttt
    57661 ggttttaaag aattgcgtcg cagagatttt ccagaccatg ccttcacgat tgtctatcta
    57721 ggtcttgagg gtgatgatta tgagttggag ttgacttata actacgatca cggtccgtat
    57781 gtggttggtg atgggtttgc ccatatcgcc ctcagtacac ctgatcttga ggcacttcat
    57841 caagaacaca gtgcaaaagg ctatgaagtg actgagccaa atggtctacc aggaactaca
    57901 ccaaactatt actttgttaa agaccctgat ggatataagg tcgaagtcat tcgtgaaaaa
    57961 taatttcgtg caagttaagt agaatattcg ttttctactt gacttttttt agctgaaaga
    58021 gtatactaat aaaaattcaa cctttaaggg gagtccagag agactcacaa ggtgtcagat
    58081 aaaagaatag tgcaattttc tagaggagac cttttgagtg tgctctcttg tgttgtacga
    58141 ttttaactga ggccttgcac tagcaaggtc ttttctttat ctggtcccct taaaatttaa
    58201 ggaggaaaag ttatgaatct cacatgtaag aagcgtttgg gtgtcattcg gttggaaacc
    58261 atgaaggtgg ttgcacaaga ggaaatcgcg ccagcaatct ttgaattagt cctagaagga
    58321 gaaatggttg aagccatgcg agcaggccaa tttcttcatc tgcgtgtacc agacgatgcc
    58381 catctcttac gtcgtcctat ttcaatttcg tctattgaca aggcaaacaa gcagtgtcac
    58441 ctcatttatc ggattgaggg agctgggact gcaatttttt caaccttaag tcagggagac
    58501 actcttgatg tgatggggcc tcagggaaat ggttttgact tgtctgacct tgatgagcag
    58561 aatcaggttc tccttgttgg tggtgggatt ggtgttccac ccttgcttga ggtggccaag
    58621 gaattgcatg aacgtggagt gaaagtagtg acagtcctcg gttttgctaa taaggatgct
    58681 gttattttga aaacggaatt ggctcagtat ggtcaggtct ttgtaacgac agatgatggt
    58741 tcttatggca tcaagggaaa tgtttccgtt gttatcaatg atttagatag tcagtttgat
    58801 gctgtttact cgtgtggggc tccaggaatg atgaagtata tcaatcaaac ctttgatgat
    58861 cacccaagag cctatttatc tctggaatct cgtatggctt gtgggatggg agcttgctat
    58921 gcctgtgttc taaaagtacc agaaagcgag acggtcagcc aacgcgtctg tgaagatggt
    58981 cctgttttcc gcacaggaac agttgtatta taaggagaaa attatgacta caaatcgatt
    59041 acaagtgtct ctacctggtt tggatttgaa aaatccgatt attccagcat caggctgttt
    59101 tggctttgga caagagtatg ccaagtacta tgatttaaac cttttaggtt ctattatgat
    59161 caaggcgaca acccttgaac cacgttttgg gaacccaact ccaagagtag cagagacgcc
    59221 tgctggtatg ctcaatgcaa ttggcttgaa aaatcctggt ttagaggttg ttttggctga
    59281 aaagctacct tggctggaaa gagaatatcc aaatcttcct attattgcca atgtagctgg
    59341 tttttcaaaa caagagtatg cagctgtttc tcatgggatt tccaaggcaa ctaatgtaaa
    59401 agctatcgag ctcaatattt cttgtcccaa tgttgaccac tgtaatcatg gacttttgat
    59461 tggtcaagat ccagatttgg cttatgatgt ggtgaaagca gctgtggaag cctcagaagt
    59521 gccagtttat gtcaaattaa ccccgagtgt gaccgatatc gttactgtcg caaaagctgc
    59581 agaagatgcg ggagcaagtg gcttgaccat gatcaatact ctggttggaa tgcgctttga
    59641 cctcaaaacc agaaaaccaa tcttggccaa tggaacaggt ggaatgtcag gtccagcagt
    59701 ctttccagta gccctcaaac tcatccgcca agtagcccaa acaacagacc tgcctatcat
    59761 tggaatgggg ggagtggatt cggctgaagc tgccctagaa atgtatctgg ctggggcatc
    59821 tgctatcgga gttggaacag ctaactttac caatccttat gcctgccctg acatcatcga
    59881 aaatttacca aaagtcatgg ataaatacgg tattagcagt ctggaagaac tccgtcagga
    59941 agtaaaagag tctctgaggt aaactgcaat caatctgttc ttgatttttt attagtttgt
    60001 aatatgaatt taggagaatt ttggtacaat aaaataaata agaacagagg aagaaggtta
    60061 atgaagaaag taagatttat ttttttagct ctgctatttt tcttagctag tccagagggt
    60121 gcaatggcta gtgatggtac ttggcaagga aaacagtatc tgaaagaaga tggcagtcaa
    60181 gcagcgaatg agtggatttt tgatactcat tatcaatctt ggttctatat aaaagcagat
    60241 gctaactatg ctgaaaatga atggctaaag caaggtgacg actattttta cctcaaatct
    60301 ggtggctata tggccaaatc agaatgggta gaagacaagg gagcctttta ttatcttgac
    60361 caagatggaa agatgaaaag aaatgcttgg gtaggaactt cctatgttgg tgcaacaggt
    60421 gccaaagtaa tagaagactg gatctatgat tctcaatacg atgcttggtt ttatatcaaa
    60481 gcagatggac agcacgcaga gaaagaatgg ctccaaatta aagggaagga ctattatttc
    60541 aaatccggtg gttatctact gacaagtcag tggattaatc aagcttatgt gaatgctagt
    60601 ggtgccaaag tacagcaagg ttggcttttt gacaaacaat accaatcttg gttttacatc
    60661 aaagaaaatg gaaactatgc tgataaagaa tggattttcg agaatggtca ctattattat
    60721 ctaaaatccg gtggctacat ggcagccaat gaatggattt gggataagga atcttggttt
    60781 tatctcaaat ttgatgggaa aatagctgaa aaagaatggg tctacgattc tcatagtcaa
    60841 gcttggtact acttcaaatc cggtggctac atggcagcca atgaatggat ttgggataag
    60901 gaatcttggt tttacctcaa gtttgatggc aaaatggctg aaaaagaatg ggtctacgat
    60961 tctcatagtc aagcctggta ctacttcaaa tccggtggtt acatgacagc caatgaatgg
    61021 atttgggata aggaatcttg gttttatctc aaatctgatg ggaaaatagc tgaaaaagaa
    61081 tgggtctacg attctcatag tcaagcttgg tactacttca aatccggtgg ttacatgaca
    61141 gccaatgaat ggatttggga taaggaatct tggttttacc tcaagtctga tggcaaaatg
    61201 gctgaaaaag aatgggtcta cgattctcat agtcaagcct ggtactactt caaatctggt
    61261 ggctacatgg cgaaaaatga gacagtagat ggttatcagc ttggaagcga tggtaaatgg
    61321 cttggaggaa aagctacaaa taaaaatgct gcttactatc aagtagtgcc tgtcacagcc
    61381 aatgtttatg attcagatgg tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg
    61441 ctagataagg atagaaaaag tgatgacaag cgcttggcta ttactatttc tggtttgtca
    61501 ggctatatga aaacagaaga tttacaagcg ctagatgcta gtaaggactt tatcccttat
    61561 tatgagagtg atggccaccg tttttatcac tatgtggctc agaatgctag tatcccagta
    61621 gcttctcatc tttctgatat ggaagtaggc aagaaatatt attcggcaga tggcctgtat
    61681 tttgatggtt ttaagcttga gaatcccttc cttttcaaag atttaacaga ggctacaaac
    61741 tacagtgctg aagaattgga taaggtattt agtttgctaa acattaataa tagccttttg
    61801 gagaacaagg gcgctacttt taaggaagcc gaagaacatt accatatcaa tgccctttat
    61861 ctccttgccc atagtgccct agaaagtaac tggggaagaa gtaaaattgc caaagataag
    61921 aataatttct ttggcattac agcctatgat acgacccctt acctttctgc caagacattt
    61981 gatgatgtgg ataagggaat tttaggtgca accaagtgga ttaaggaaaa ttatatcgat
    62041 aggggaagaa ctttccttgg aaacaaggct tctggtatga atgtggaata tgcttcagac
    62101 ccttattggg gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa gctaggtggc
    62161 aaagattagt actataagtg aatatgattt gagtgaatag taagttaaaa atcctgattt
    62221 caagtaaaat caggattttt tcatggatgc aatttttttg gagtctggtg tgacgcggag
    62281 ggtcttttgt cctgtgtaag tgacaaagcc gggttttcca ccagttggtt tattgagttt
    62341 tttgacttca atcatatcta cctgcaccag attcgacagg cgcccttgag agaagtaggc
    62401 agctaactct gctgcgtctg tcttgactgc atcagatggg tcaagatttc ctgagatgac
    62461 aacatggctt ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc gggccatttt
    62521 aaaggtcaat tcctcatttt gaagattgtt tcgtccgaca tagatgatgg ttttgccatc
    62581 gcttgctaga tattgttcta gttttttgcg tttctggatt ttctcccgtt gtcttctgcg
    62641 gataaaacct gtttgaatca attcttcacg gatttcagcg atttcttcca gtccagcttg
    62701 gttgaggacg gtttctacac tttccagata gagaatagtg gctttggttt cttcaatcaa
    62761 atcagtcaag tatttgacag cttctttgag tttctgatac cttttaaaat agcgttgggc
    62821 attctggttg ggagtcagag ccttatcaag cgcaatcatg ataggttggt tggtatagta
    62881 gttgtctagg ataacctggt cttggtcgtt aggcacttgg tggaggaagg ttgtcagcaa
    62941 ttctcctttt tgacgaaatt cttcagcgtt gtctgtcgcc agtaactctt tttcctgttt
    63001 tttgagtttg tgtcggtttt tctgaagttc attttcaaca cgacgaatca gttcactggc
    63061 ctgctgtttg acgcggtcgc gctcagcctt atccttatag taggtgtcca acaaatcaga
    63121 aagatttgca aaaggctctc ccacctgatt tgcaaaagga actggactga aggaagtctc
    63181 agtcaagcat ggcttggttt cttgattgaa aaaatttcgg aaagcggaaa gtttttcact
    63241 aaccagtatc ctttccaatt catttgccgt atcgcgtccc agaccttgaa agaggctttg
    63301 aagatttttt gctgttagtt cttgggtttg taggatttca aagagctttt catccttgat
    63361 agtaaaagga ttgagagatt ttgtacttgg cggagcgata taggtcgatc ctggaagtaa
    63421 ggtgcggtag ctattttgtg aaaaaccgac gtgtttgata acttcgagga ttttatgact
    63481 gcttttatcg accagtagaa tattactgtg ttttcccata atttcaataa tcaaggtagc
    63541 ctggatatgg tctccaatct cgtttttatt ggaaactgta atttccacaa tacggtcatt
    63601 ttccacttgc tcaatcgact caatcagggc cccctgcaaa tactttctca aaaccatgat
    63661 aaaggtagaa ggttgagctg gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa
    63721 aactggatgg gcagaaagga gcaggcgatg gctttggcga ttgctgcgga tttgcaagac
    63781 caactcttgt tcaaaaggct gattgatttt ctggatgcga ccattcacta attcgcttcg
    63841 caattcctca actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc tctccttgtg
    63901 attgtattcc atagtattat atcaaaaagg tagaataaaa tcatggaaat gtggtataat
    63961 aaagccaagt aaagagaaac gagaagcaca tgtatattga aatggtagat gaaactggtc
    64021 aagtttcaaa agaaatgttg caacaaaccc aagaaatttt ggaatttgca gcccaaaaat
    64081 taggaaaaga agacaaggag atggcagtca cttttgtgac caatgagcgt agtcatgaac
    64141 ttaatctgga gtaccgtgac accgaccgtc cgacagatgt catcagcctt gagtataaac
    64201 cagaattgga aattgccttt gacgaagagg atttgcttga aaatccagaa ttggcagaga
    64261 tgatgtctga gtttgatgcc tatattgggg aattgttcat ctctatcgat aaggctcatg
    64321 agcaggccga agaatatggt cacagctttg agcgtgagat gggcttcttg gcagtacacg
    64381 gctttttaca tattaacggc tatgatcact atactccgga agaagaagcg gagatgttcg
    64441 gtttacaaga agaaattttg acagcctatg gactcacaag acaataaacg aaaatggaaa
    64501 aatcgtgact tgatatccag tttagaattt gccatcacag ggatttttac tgctatcaag
    64561 gaagaacgaa atatgcgaaa acatgcagtg acggctctag tggtcatcct tgcaggtttt
    64621 gtttttcagg tgtcacgaat cgaatggctc tttctcctat tgagtatttt cttggtagta
    64681 gcctttgaga ttatcaactc tgctattgaa aatgtggtgg atttggccag tcactatcac
    64741 ttttccatgc tggctaaaaa tgccaaggat atggcggccg gcgcggtatt agtggtttct
    64801 cttttcgcag ccttaacagg cgcattgatt tttctcccac gaatctggga tttattattt
    64861 taaacagtaa gaggaaatta tgacttttaa atcaggcttt gtagccattt taggacgtcc
    64921 caatgttggg aagtcaacct ttttaaatca cgttatgggg caaaagattg ccatcatgag
    64981 tgacaaggcg cagacaacgc gcaataaaat catgggaatt tacacgactg ataaggagca
    65041 aattgtcttt atcgacacac cagggattca caaacctaaa acagctctcg gagatttcat
    65101 ggttgagtct gcctacagta cccttcgcga agtggacacc gttcttttca tggtgcctgc
    65161 tgatgaagcg cgtggtaagg gggacgatat gattatcgag cgtctcaagg ctgccaaggt
    65221 tcctgtgatt ttggtggtga ataaaatcga taaggtccat ccagaccagc tcttgtctca
    65281 gattgatgac ttccgtaatc aaatggactt taaggaaatt gttccaatct cagcccttca
    65341 gggaaataac gtgtctcgtc tagtggatat tttgagtgaa aatctggatg aaggtttcca
    65401 atatttcccg tctgatcaaa tcacagacca tccagaacgt ttcttggttt cagaaatggt
    65461 tcgcgagaaa gtcttgcatc taactcgtga agagattccg cattctgtag cagtagttgt
    65521 tgactctatg aaacgagacg aagagacaga caaggttcac atccgtgcaa ccatcatggt
    65581 cgagcgcgat agccaaaaag ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat
    65641 cggtagcatg gcccgtcgtg atatcgaact catgctagga gacaaggtct tcctagaaac
    65701 ctgggtcaag gtcaagaaaa actggcgcga taaaaagcta gatttggctg actttggcta
    65761 taatgaaaga gaatactaag tagaggtagg ctcatgcctg cttcttgttt ttacagaagg
    65821 aggacttatg cctgaattac ctgaggttga aaccgtttgt cgtggcttag aaaaattgat
    65881 tataggaaag aagatttcga gtatagaaat tcgctacccc aagatgatta agacggattt
    65941 ggaagagttt caaagggaat tgcctagtca gattatcgag tcaatgggac gtcgtggaaa
    66001 atatttgctt ttctgcctga cagacaaggt cttgatttcc catttgcgga tggagggcaa
    66061 gtatttttat tatccagacc aagtgcctga acgcaagcat gcccatgttt tcttccggtt
    66121 tgaagatggg ggcacgcttg tttatgagga tgtacgcaag tttggaacca tggaactctt
    66181 ggtgcctgac cttttagacg cctactttat ttctaaaaaa ttaggtcctg aaccaagcga
    66241 acaagacttt gatttacagg tctttcaagc tgcccttgcc aagtccaaaa agcctatcaa
    66301 atcccatctc ctagaccaga ccttggtagc tggacttggc aatatctatg tggatgaggt
    66361 tctctggcga gctcaggttc atccagctag accttcccag actttgacag cagaagaagc
    66421 gactgccatt catgaccaga ccattgctgt tttgggccag gctgttgaaa aaggtggctc
    66481 caccattcgg acttatacca atgcctttgg ggaagatgga agcatgcagg actttcatca
    66541 ggtctatgat aagactggtc aagaatgtgt acgctgtggt accatcattg agaaaattca
    66601 actaggcgga cgtggaaccc acttttgtcc aaactgtcaa aggagggact gatgggaaaa
    66661 atcatcggaa tcactggggg aattgcctca ggtaagtcaa ctgtgacaaa ttttctaaga
    66721 cagcaaggct ttcaagcagt ggatgccgac gcagtcgtcc accaactaca gaaacctggt
    66781 ggtcgtctgt ttgaggctct agtacagcac tttgggcaag aaatcattct tgaaaacgga
    66841 gaactcaatc gccctctcct agctagtctc atcttttcaa atcctgaaga gaaaaaatgg
    66901 tctaagcaaa ttcaagggga gattatccgt gaggaactgg ctactttgag agaacagttg
    66961 gctcagacag aagagatttt cttcatggat attcccctac tttttgagca ggactacagc
    67021 gattggtttg ctgagacttg gttggtctat gtggaccgag atgcccaagt agaacgctta
    67081 atgaaaaggg accagttgtc caaagatgaa gctgagtctc gtctggcagc ccagtggcct
    67141 ttagaaaaaa agaaagattt ggccagccag gttcttgata ataatggcaa tcagaaccag
    67201 cttcttaatc aagtgcatat ccttcttgag ggaggtaggc aagatgacag agattaactg
    67261 gaaggataat ctgcgtattg cctggtttgg taattttctg acaggagcca gtatttcttt
    67321 ggttgtacct tttatgccca tcttcgtgga aaatctaggt gtagggagtc agcaagtcgc
    67381 tttttatgca ggcttagcaa tttctgtctc tgctatttcc gcggcgctct tttctcctat
    67441 ttggggtatt cttgctgaca aatacggccg aaaacccatg atgattcggg caggtcttgc
    67501 tatgactatc actatgggag gcttggcctt tgtcccaaat atctattggt taatctttct
    67561 tcgtttacta aacggtgtat ttgcaggttt tgttcctaat gcaacggcac tgatagccag
    67621 tcaggttcca aaggagaaat caggctctgc cttaggtact ttgtctacag gcgtagttgc
    67681 aggtactcta actggtccct ttattggtgg ctttatcgca gaattatttg gcattcgtac
    67741 aattttctta ctggttggta gttttctatt tttagctgct attttgacta tttgctttat
    67801 caaggaagat tttcaaccag tagccaagga aaaggccatt ccaacaaagg aattatttac
    67861 ctcggttaaa tatccctatc ttttgctcaa tctcttttta accagttttg tcatccaatt
    67921 ttcagctcaa tcgatcggcc ctattttggc tctttatgta cgcgacttag ggcagacaga
    67981 gaatcttctt tttgtctctg gtttgattgt gtccagtatg ggcttttcca gtatgatgag
    68041 tgcaggagtt atgggaaagc taggtgacaa ggtgggcaat catcgtctct tggttgtcgc
    68101 ccaattttat tcagtcatca tctatctcct ctgtgccaat gcctctagcc cccttcaact
    68161 aggactctat cgattcctct ttggcttggg aaccggtgcc ttgattcccg gggttaatgc
    68221 cctactcagc aaaatgactc ccaaagccgg catttcgagg gtctttgcct tcaatcaggt
    68281 attcttttat ctgggaggtg ttgttggtcc catggcaggt tctgcagtag caggtcaatt
    68341 tggctaccaa gctgtctttt atgcgacaag cctttgtgtt gcctttagtt gtctctttaa
    68401 cctgattcga tttcgaacat tattaaaagt aaaggaaatc tagtgcgagt aaaaattaat
    68461 ctcaaatgct cctcttgtga cagtatcaat tacctaacca gtaaaaattc aaaaacccat
    68521 ccagacaaga ttgaggtttt aaagtattgt cccaaggaaa gaaaagtaac cctacatctt
    68581 gaatctaagt agattttatg gtaaaataat agggatttta aggagtttga tatgtataac
    68641 ctattattaa ccattttatt agtattatct gttgtgattg tgattgcaat tttcatgcaa
    68701 ccaaccaaaa accaatccag caatgtattt gatgccagtt caggtgattt gtttgaacgc
    68761 agtaaagctc gcggttttga agctgtaatg cagcgtttga cagggatttt agtctttttc
    68821 tggctagcca ttgccttagc attgacggta ttatcaagta gataagaaaa taatgggcag
    68881 gactaggtct ttgcctcttt ttatttttaa aggatgtttg agaaggtttt acagtaaaag
    68941 aaaattaaaa aatctagaaa gaaaatatga aagatagaat aaaagaatat ttacaagaca
    69001 agggaaaggt gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt
    69061 ttcgtgagtt gattaaaacc ttgtccttaa tggaaagaaa gcaccagatt cgctttgaag
    69121 aagatggcag tctgaccttg gaagccaaga aaaaacatga gattaccctc aaggggattt
    69181 ttcatgccca taaaaatggt tttggttttg tcagcctaga aggggaagag gacgaccttt
    69241 ttgtagggaa aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta
    69301 agaaagtcgc tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag
    69361 aacacagttt gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg
    69421 ctggctatat tcgttcaaaa aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag
    69481 ccctaaagtt agaaggaaca gaagttctca aggtctttat cgataaatac ccaagcaaga
    69541 aacatgattt ctttgtcgcg agtgttctcg atgtagtggg acactcaacg gatgtcggaa
    69601 ttgatgttct tgaggtcttg gaatcaatgg acattgtatc cgagtttcca gaagctgttg
    69661 ttaaggaagc agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg
    69721 atctaagaga tgaaattacc tttaccattg acggtgcgga tgccaaggac ttggacgatg
    69781 cagtgcatat caaggctctg aaaaatggca atctggagct tggggttcac atcgcagatg
    69841 tttcttatta tgtgaccgag gggtctgccc ttgacaagga agcccttaac cgtgcgactt
    69901 ctgtttacgt gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct
    69961 gctctctcaa tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac
    70021 atggtcgtgt ggtcaactat accattacac aaacagttat caagaccagt tttcgtatga
    70081 cctatagcga tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaatatcata
    70141 aaattgtacc aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg
    70201 tgaaacgtgg agctctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag
    70261 gtaagcctgt tgatatcgtt cttcggcagc gtggcattgc cgagcggatg attgagtctt
    70321 ttatgttgat ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta
    70381 tctatcgaat tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt
    70441 cgagttttgg cttgcgcatt tatgggactg ccagtgagat tagtcaggag gcacttcaag
    70501 acatcatgcg tgctgttgag ggagaacctt atgcagatgt attgtccatg atgcttcttc
    70561 gctctatgca gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg
    70621 actattatac tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta
    70681 tgattcggga ttacggccgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag
    70741 agattgcgac ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg
    70801 aagccatgaa aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg
    70861 tatcaagtat tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga
    70921 ttcacatcac taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg
    70981 gagaaaaatc aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag
    71041 cggataaaat gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg
    71101 aaaaaggctt gaaacagtct agtcatagtg gcagagggcg tggttcaaat cgtcgttcgg
    71161 ataagaagga agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa
    71221 aagacaagaa gaaaaaagga aagaaacctt tttacaagga agtagctaag aaaggagcca
    71281 agcatggcaa agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat
    71341 acaatcgtag atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta
    71401 cgagctgctc gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg
    71461 ctgagcaatg ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa
    71521 cgtcgtcgta aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa
    71581 gggacaggaa tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt
    71641 cttttaggac ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt
    71701 gagcaaaatc gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa
    71761 ttgaaaatgg aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg
    71821 cctgaagctg ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc
    71881 ttgaagggag ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac
    71941 ctctttgaag caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa
    72001 gctgccacag atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt
    72061 gctaaaaaat acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg
    72121 aaacaaggga aagctttgga tttgggttgt ggtcaggggc gtaattctct ttttctagcc
    72181 cagcaagatt ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa
    72241 agcattgtgg agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct
    72301 agcattgaac aagaatatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg
    72361 gaccgcattc cagctattat tcaaaatatg caggagaaaa ccagtgttgg tggttacaac
    72421 cttatcgttt gtgccatgga cacggaggat tatccttgct cggttaactt cccattcacc
    72481 tttaaagaag gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa
    72541 aatccaggcc atttgcaccg tcgcgatgag aatggcaatc gtattcaact acgctttgcg
    72601 accttactag ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt
    72661 ttttcttttt tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct
    72721 aggggagaat tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca
    72781 gcttgtggag gccagatcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat
    72841 aaattcttaa aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat
    72901 aaggaatccc tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt
    72961 tcagaactta aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag
    73021 tgtagtccct tgcctcagaa agtccttaaa gagcgaagtg agggctatcg tagtcagggt
    73081 taccaagtac tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta
    73141 cagcaaggtt ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag
    73201 gaaaaacaag ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat
    73261 tatcaaatca aggaattttc ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc
    73321 tataagaaac aaaaaatatc tcattttaca gtttctgagg acaaggacat ctgtcgctat
    73381 atccggcaac aactttatta tcaaaatctc ttttggatga aagaacaagc agaagcctat
    73441 caaaagggag aaaatatcct gacttatgga ctgaaagaat ggtatccaca aattcgacca
    73501 atagtgggca aatttttcca gattgaacaa gacttgacta gctattatca gcacttttat
    73561 acctattacc aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc agccttttat
    73621 cagcaatatt tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct aatggtatta
    73681 caaagaaatg aaataaatga aaaagataca tgggatctat caacgatcta cccaactgac
    73741 caggcttggg aagaagcctt aaaagattta acagaacaat tggagacagt agtccagtat
    73801 gaaggccatc tcttggatag tgcggataac ctactagaaa tcactgaatt ttctcttgaa
    73861 atggaacgcc agatggagaa gctttacgtt tatgctcata tgaagaatga ccaggataca
    73921 cgtgaagcta agtatcaaga gtactatgcc aaggccatga cactctacag ccagttagac
    73981 caagcctttt cattctatga gcctgaattt atggagatta gcgaaaagca gtatgctgac
    74041 tttttagaag ctcaaccaaa gctgcaggtt tatcaacact attttgacaa gctcttgcaa
    74101 ggcaaggatc acgttctttc acaacgtgaa aaagaattat tggctggagc tggagaaatc
    74161 tttggttcag caagtgaaac cttcgctatc ttggacaatg cggatattgt gttcccttat
    74221 gtactagacg atgatggtaa agaagttcaa ctatctcatg ggacttacac acgtttgatg
    74281 gagtctaaaa aacgtgaggt tcgccgtggt gcctatcaag ctctttatgc gacttacgaa
    74341 caattccaac acacctatgc caaaaccttg caaaccaatg ttaaggtgca aaattaccgt
    74401 gctaaagttc gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca
    74461 gaaagtgttt atgacaattt ggtagcagca gttcgcaagc atttgccact cttacatcgc
    74521 tatcttgagc ttcgttcaaa aatcttgggg atttcagatc tcaagatgta cgatgtctac
    74581 acaccgcttt catctgttga atacagtttt acctaccaag aagccttgaa aaaagcagaa
    74641 gatgctttgg cagtcttggg tgaggattac ttgagccgtg tcaaacgtgc cttcagcgag
    74701 cgttggattg atgtttacga aaatcaaggc aagcgttcag gtgcctactc tggtggttct
    74761 tacgatacca atgcctttat gcttctcaac tggcaggaca atctggacaa tctctttact
    74821 cttgttcatg aaacaggtca cagtatgcat tcaagctata ctcgtgaaac tcagccttat
    74881 gtttacggag attactctat ctttttggct gagattgcct caactaccaa tgaaaatatc
    74941 ttgacggaga aattattgga agaagtggaa gacgacgcaa cacgctttgc tattctcaat
    75001 aacttcctag atggtttccg tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa
    75061 cacgccattc accaagcaga tcaaaatggg gaggtcttga caagcgattt cctaaataaa
    75121 ctctacgcag acttgaacca agagtattat ggtttgagta aggaagacaa tcctgaaatc
    75181 caatacgagt gggctcgcat tccacacttc tactataact actatgtata tcaatattca
    75241 actggctttg cggccgcctc agccttggct gaaaaaattg tccatggtag tcaagaagac
    75301 cgtgaccgct atatcgacta cctcaaggca ggtaagtcgg actatccact taatgtcatg
    75361 agaaaagctg gtgttgatat ggagaaggaa gactacctca acgatgcctt tgcagtcttt
    75421 gaacgccgtt taaatgagtt tgaagccctt gttgaaaaat taggattggc ataaaatggt
    75481 tgaatcgtat agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat
    75541 tgtagacttg atgcgtcagc gtcaaaagca agtcacaggt ttcttgaaag aattggaaga
    75601 ctttgcccgc aaggaaaata ttcctattat tccccatgaa acggttgctt atttccgttt
    75661 tcttatggaa accatgcagc ctaaaaatat tctggaaatt gggacggcta tcggtttttc
    75721 agctctcttg atggctgaac atgcgccaaa tgctaagatt acaactattg atcgtaatcc
    75781 agaaatgatt ggttttgcca aggaaaattt tgcccagttt gacagtcgca agcaaatcac
    75841 tctcctagag ggagatgcgg tggatgtctt atctacactg acagagtctt atgatttcgt
    75901 ctttatggat tctgccaagt ctaaatacat cgtctttctg ccagaaattc tcaaacattt
    75961 ggaagttggt ggtgtggttg tcttggatga tattttccaa ggcggtgatg ttgccaagga
    76021 cattatggaa gtccgtcgag gtcagcgaac catctatcga ggccttcaaa gactatttga
    76081 cgcaacctta gacaattcag aactcaccgc aacattagtg cctttaggag atggtattct
    76141 catgcttcgt aaaaatgtag cagatgttca attgcctgac agcgaatgat tttcagaaaa
    76201 atttaagaaa aaatagtaaa atagatagag taacacttat ctcaaaggag tagacatgaa
    76261 gaaaaaatta ttggcaggtg ccatcacact attatcagta gcaactttag cagcttgttc
    76321 gaaagggtca gaaggagcag accttatcag catgaaaggg gatgtcatca cagaacatca
    76381 attttatgag caagtgaaaa acaacccttc agcccaacaa gtcttgttaa atatgaccat
    76441 ccaaaaagtt tttgaaaaac aatatggctc agagcttgat gataaagagg ttgatgatac
    76501 tattgccgaa gaaaaaaaac aatatggcga aaactaccaa cgtgtcttgt cacaagcagg
    76561 tatgactctt gaaacacgta aagctcaaat tcgtacaagt aaattagttg agttggcagt
    76621 taagaaggta gcagaagctg aattgacaga tgaagcctat aagaaagcct ttgatgagta
    76681 cactccagat gtaacggctc aaatcatccg tcttaataat gaagataagg ccaaagaagt
    76741 tctcgaaaaa gccaaggcag aaggtgctga ttttgctcaa ttagccaaag ataattcaac
    76801 tgatgaaaaa acaaaagaaa atggtggaga aattaccttt gattctgctt caacagaagt
    76861 acctgagcaa gtcaaaaaag ccgctttcgc tttagatgtg gatggtgttt ctgatgtgat
    76921 tacagcaact ggtacacaag cttacagtag ccaatattac attgtaaaac tcactaagaa
    76981 aacagaaaaa tcatctaata ttgatgacta caaagaaaaa ttaaaaactg ttatcttgac
    77041 tcaaaaacaa aatgattcaa catttgttca aagcattatc ggaaaagaat tgcaagcagc
    77101 caatatcaag gttaaggacc aagccttcca aaatatcttt acccaatata tcggtggtgg
    77161 agattcaagc tcaagcagta gtacatcaaa cgaatagtcc aaatcaatga gtcagggaaa
    77221 aaaactcgac ttcaggaaaa aatgaagcaa acattcccac aataaaacgc atagtacaag
    77281 gtttgtactg cccccaaaag ttagacaatt aatttatctg aaggatttag ttctgtattg
    77341 cacagggcta agtcctttta gttttacctt aattctctta ttgttctaat aatcaatata
    77401 gtctataatg gcttgttcca attgattaag tgatttaaat gttttctcat agccataaaa
    77461 catttcggat tttaaaatgc caaagaaaga ttccatccta ccgttgtctt ggctgtttcc
    77521 tttgcgtgac atagatgctt gaattccctt actctctagg aagcgatgat aagaatcgtg
    77581 ttggtattgc cagccttggt cactatggag aatcgtattc tcgtagtgct tctctttgaa
    77641 tgcctgttcc aacattaacg atcaatcaat ttaatcatgt acctaatatt agaattgttt
    77701 atcccaaatt tatttgaaag cttctctaa
//
LOCUS       DAVKWS010000004        65205 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKWS010000004 DAVKWS010000000
VERSION     DAVKWS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.004"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..999)
                     /gene="pavB"
                     /locus_tag="VW852_000306"
     CDS             complement(<1..999)
                     /gene="pavB"
                     /locus_tag="VW852_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV0271506.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGV"
     gene            complement(1115..1525)
                     /locus_tag="VW852_000307"
     CDS             complement(1115..1525)
                     /locus_tag="VW852_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV0271507.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            complement(1592..1765)
                     /locus_tag="VW852_000308"
     CDS             complement(1592..1765)
                     /locus_tag="VW852_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV0271508.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYTMMHS
                     LIYRTDLLRASQF"
     gene            complement(1827..1985)
                     /locus_tag="VW852_000309"
     CDS             complement(1827..1985)
                     /locus_tag="VW852_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271509.1"
                     /translation="MKIKDQTRKLATGCLKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRC"
     gene            complement(2120..2785)
                     /locus_tag="VW852_000310"
     CDS             complement(2120..2785)
                     /locus_tag="VW852_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV0271510.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(2799..4178)
                     /locus_tag="VW852_000311"
     CDS             complement(2799..4178)
                     /locus_tag="VW852_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0271511.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            complement(4791..5555)
                     /locus_tag="VW852_000312"
     CDS             complement(4791..5555)
                     /locus_tag="VW852_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV0271512.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(5772..6329)
                     /locus_tag="VW852_000313"
     CDS             complement(5772..6329)
                     /locus_tag="VW852_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEV0271513.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            complement(6435..7289)
                     /locus_tag="VW852_000314"
     CDS             complement(6435..7289)
                     /locus_tag="VW852_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0271514.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            complement(7645..13215)
                     /gene="zmpC"
                     /locus_tag="VW852_000315"
     CDS             complement(7645..13215)
                     /gene="zmpC"
                     /locus_tag="VW852_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HEV0271515.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            complement(13667..14880)
                     /locus_tag="VW852_000316"
                     /pseudo
     CDS             complement(13667..14880)
                     /locus_tag="VW852_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
     gene            complement(14997..16034)
                     /locus_tag="VW852_000317"
     CDS             complement(14997..16034)
                     /locus_tag="VW852_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV0271516.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            complement(16150..17316)
                     /locus_tag="VW852_000318"
     CDS             complement(16150..17316)
                     /locus_tag="VW852_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV0271517.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            complement(17615..18019)
                     /locus_tag="VW852_000319"
     CDS             complement(17615..18019)
                     /locus_tag="VW852_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0271518.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            complement(18026..18841)
                     /locus_tag="VW852_000320"
     CDS             complement(18026..18841)
                     /locus_tag="VW852_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0271519.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            complement(18828..19733)
                     /locus_tag="VW852_000321"
     CDS             complement(18828..19733)
                     /locus_tag="VW852_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0271520.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            complement(19761..20237)
                     /locus_tag="VW852_000322"
     CDS             complement(19761..20237)
                     /locus_tag="VW852_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0271521.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            complement(20234..22021)
                     /locus_tag="VW852_000323"
     CDS             complement(20234..22021)
                     /locus_tag="VW852_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV0271522.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            22369..23085
                     /locus_tag="VW852_000324"
     CDS             22369..23085
                     /locus_tag="VW852_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0271523.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            23400..27338
                     /gene="strH"
                     /locus_tag="VW852_000325"
     CDS             23400..27338
                     /gene="strH"
                     /locus_tag="VW852_000325"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0271524.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(27393..28691)
                     /gene="purB"
                     /locus_tag="VW852_000326"
     CDS             complement(27393..28691)
                     /gene="purB"
                     /locus_tag="VW852_000326"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV0271525.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(28754..28981)
                     /locus_tag="VW852_000327"
     CDS             complement(28754..28981)
                     /locus_tag="VW852_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV0271526.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            complement(28991..30082)
                     /gene="purK"
                     /locus_tag="VW852_000328"
     CDS             complement(28991..30082)
                     /gene="purK"
                     /locus_tag="VW852_000328"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV0271527.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            complement(30069..30557)
                     /gene="purE"
                     /locus_tag="VW852_000329"
     CDS             complement(30069..30557)
                     /gene="purE"
                     /locus_tag="VW852_000329"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV0271528.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            complement(30960..32222)
                     /gene="purD"
                     /locus_tag="VW852_000330"
     CDS             complement(30960..32222)
                     /gene="purD"
                     /locus_tag="VW852_000330"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV0271529.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            complement(32344..33891)
                     /gene="purH"
                     /locus_tag="VW852_000331"
     CDS             complement(32344..33891)
                     /gene="purH"
                     /locus_tag="VW852_000331"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV0271530.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDMTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADNIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            complement(33916..34425)
                     /locus_tag="VW852_000332"
     CDS             complement(33916..34425)
                     /locus_tag="VW852_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0271531.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            complement(34509..35054)
                     /gene="purN"
                     /locus_tag="VW852_000333"
     CDS             complement(34509..35054)
                     /gene="purN"
                     /locus_tag="VW852_000333"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV0271532.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            complement(35051..36073)
                     /locus_tag="VW852_000334"
     CDS             complement(35051..36073)
                     /locus_tag="VW852_000334"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV0271533.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            complement(36110..37552)
                     /locus_tag="VW852_000335"
     CDS             complement(36110..37552)
                     /locus_tag="VW852_000335"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV0271534.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGKLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEDRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            complement(37645..41370)
                     /locus_tag="VW852_000336"
     CDS             complement(37645..41370)
                     /locus_tag="VW852_000336"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV0271535.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNVGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVAIVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELETALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEVANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            complement(41427..42134)
                     /locus_tag="VW852_000337"
     CDS             complement(41427..42134)
                     /locus_tag="VW852_000337"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV0271536.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            complement(42304..43653)
                     /locus_tag="VW852_000338"
     CDS             complement(42304..43653)
                     /locus_tag="VW852_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV0271537.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLINQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            complement(43666..45819)
                     /locus_tag="VW852_000339"
     CDS             complement(43666..45819)
                     /locus_tag="VW852_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0271538.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRDRFAEEWTGVTLFMAPSPDYKPHKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            complement(46534..46764)
                     /gene="blpU"
                     /locus_tag="VW852_000340"
     CDS             complement(46534..46764)
                     /gene="blpU"
                     /locus_tag="VW852_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV0271539.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            complement(46967..47257)
                     /locus_tag="VW852_000341"
                     /pseudo
     CDS             complement(46967..47257)
                     /locus_tag="VW852_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(47303..47536)
                     /locus_tag="VW852_000342"
     CDS             complement(47303..47536)
                     /locus_tag="VW852_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0271540.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(47542..48534)
                     /gene="plsX"
                     /locus_tag="VW852_000343"
     CDS             complement(47542..48534)
                     /gene="plsX"
                     /locus_tag="VW852_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV0271541.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(48531..49301)
                     /gene="recO"
                     /locus_tag="VW852_000344"
     CDS             complement(48531..49301)
                     /gene="recO"
                     /locus_tag="VW852_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV0271542.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(49298..50467)
                     /locus_tag="VW852_000345"
     CDS             complement(49298..50467)
                     /locus_tag="VW852_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0271543.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAVVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTSQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            50616..51626
                     /locus_tag="VW852_000346"
     CDS             50616..51626
                     /locus_tag="VW852_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV0271544.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(51809..52246)
                     /locus_tag="VW852_000347"
     CDS             complement(51809..52246)
                     /locus_tag="VW852_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV0271545.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(52331..54964)
                     /gene="polA"
                     /locus_tag="VW852_000348"
     CDS             complement(52331..54964)
                     /gene="polA"
                     /locus_tag="VW852_000348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV0271546.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            55220..55570
                     /locus_tag="VW852_000349"
     CDS             55220..55570
                     /locus_tag="VW852_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0271547.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            55785..55901
                     /locus_tag="VW852_000350"
     CDS             55785..55901
                     /locus_tag="VW852_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV0271548.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            55894..56127
                     /locus_tag="VW852_000351"
     CDS             55894..56127
                     /locus_tag="VW852_000351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271549.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            56253..56534
                     /locus_tag="VW852_000352"
                     /pseudo
     CDS             56253..56534
                     /locus_tag="VW852_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(56668..57636)
                     /locus_tag="VW852_000353"
     CDS             complement(56668..57636)
                     /locus_tag="VW852_000353"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0271550.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(57781..58435)
                     /locus_tag="VW852_000354"
                     /pseudo
     CDS             complement(57781..58435)
                     /locus_tag="VW852_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(58460..58957)
                     /locus_tag="VW852_000355"
     CDS             complement(58460..58957)
                     /locus_tag="VW852_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV0271551.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(59030..60391)
                     /gene="radA"
                     /locus_tag="VW852_000356"
     CDS             complement(59030..60391)
                     /gene="radA"
                     /locus_tag="VW852_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV0271552.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(60405..60920)
                     /locus_tag="VW852_000357"
     CDS             complement(60405..60920)
                     /locus_tag="VW852_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0271553.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(60922..61365)
                     /locus_tag="VW852_000358"
     CDS             complement(60922..61365)
                     /locus_tag="VW852_000358"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV0271554.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFVQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(61425..61518)
                     /gene="ffs"
                     /locus_tag="VW852_000359"
     ncRNA           complement(61425..61518)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW852_000359"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(61551..62018)
                     /locus_tag="VW852_000360"
     CDS             complement(61551..62018)
                     /locus_tag="VW852_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV0271555.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(62219..63505)
                     /locus_tag="VW852_000361"
     CDS             complement(62219..63505)
                     /locus_tag="VW852_000361"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV0271556.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(63736..63972)
                     /locus_tag="VW852_000362"
     CDS             complement(63736..63972)
                     /locus_tag="VW852_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV0271557.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            64237..65010
                     /locus_tag="VW852_000363"
                     /pseudo
     CDS             64237..65010
                     /locus_tag="VW852_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(65129..65202)
                     /locus_tag="VW852_000364"
     tRNA            complement(65129..65202)
                     /locus_tag="VW852_000364"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65166..65168),aa:Asn,seq:gtt)
BASE COUNT        19190 a        15293 c        11988 g        18734 t
ORIGIN      
        1 gacacctgct gtgaatggac cttcaccctt ggctttttct aggtaggctg ctggaacatc
       61 gatactgtct ttaacgttat ctgcaacggc tttttgaact gtttctttag caactaatcc
      121 attgatgttg atgtctacat ttttagtagc aactagatta gtcaagtcag ctttaccgtc
      181 tttatttccg taaactttaa cagtagcttt ataagtttga gtaccattag cgcgaagttg
      241 atcaattaaa gcttgacctt gtttaccaac agtattgcca tttaagtcta cttcatagaa
      301 gtatttccct tttgttaatc cttcaagagg aggtaaagca ggatttttag cagtaccatt
      361 gtcagaccaa ggagcattat ccgaagcttt tagtaataga cgagttaaca taccatcacc
      421 agcgaatagt tcataaggaa ttacttggtt tacaccggca gtgaaaggac ctttcccttc
      481 agctttttca agataagcag caggaacatc gatgctgtct tttgtatttt ctttcactcc
      541 accttttact tgttcaggag tagtttctgc tggacttgct tgtgtcccat ccacatcagc
      601 tgtatctaca gtttctttta agacatctgt tagctctgga tttttttgag cttcttcatc
      661 tttttcaaaa agtgtatcca gactagaaga gtttgtttcc tcgctcactt cagttctttc
      721 tgtaggtgtc ggaagtgaat tcgtatttcc agtaacgccc tcaggtttcg cttgagtgag
      781 aacggtgtct cctggttttt ctgataagtc accctctttc gttcccgatg tctcttcagg
      841 taagacttga cctggggtcg gattgaccac atcggcacgt acagaacttg gctgaccaac
      901 taggacaaag aagccactag ccacaacaac tgaggcaaca ccgacactga gacggcgaat
      961 agaccaacga gtatatcttt gatttggatt gaatttcata ggattctcct tagagtcttt
     1021 ctttatttga tgactctagt ataatctcct gaaattaaaa aggactaaga aaaagttaaa
     1081 atttttctta aatccctctt ataaaacact tatattaatt aaatccataa acagatttgg
     1141 tgatttgata gacgacattg gacagtttgc gatctggcaa gacagaatgt ttggtcaaac
     1201 ggctcaacat ggtcttacga atagcctgaa agacttctgg atttttctgc tgaatatagg
     1261 tccacaattg gcgttttttt gccagatgct ccgctgttcc agatcggttg agcagggtac
     1321 tggaaatcac cgtcgtgatt tcaatatgat tcagcagata ttctcgcatt ttgggatgac
     1381 tcacttggga caaatcaagt tggtctacca agagtcgatt gaccttgagt tgctggtcaa
     1441 tgcacttaat catcacttgc tcattgacag actggtcctc acgcccaatc aaataacgat
     1501 agaaatcgac aggcagatag tacatggtct tgacctgctg aaggggcgta aagacaaaga
     1561 gattatcgac ataaaaagta tgttcaggca gttagaactg gctagcacgc aacaaatctg
     1621 tccgataaat cagcgagtgc atcatggtat actggccttt ggagaaattt ccgacctggt
     1681 cccagccaat cactagcgac tccacacagt aatgaagata ggctgctgca ttatagctag
     1741 aaatggcgat agacaataac ttcataatct gctcctttag gggactgatt ttttcttata
     1801 ctcttcgaaa atctcttcaa accgcgtcaa catcgccttg ccgtatagat gttactgact
     1861 tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agttctatcc
     1921 acaacctcaa aacagtgttt taagcagccc gtagctagtt tcctagtttg atctttgatt
     1981 ttcattgagt attactctct cttgtcactt ccttctattt taccataaag tccagccttt
     2041 gaagaacttt tactagaaga caaggggctt ctgtctctat ttgccatctt gggcatcaaa
     2101 aaagaggggt catccctctt tacgaattca atgctactag ggtatccaaa tactggttgt
     2161 tgatgactgc caaaatatag gtatctgctt tcaagaggtc atctggtcca aattcaacat
     2221 ccaatgggga attttcctgc tctcggaaac ccaaaatatt cagattgtat ttgccacgga
     2281 ggtctaattt actcagactt tgacctgccc aagactgagg aattttcatc tccacgatag
     2341 acacattttt atccaactga aagacatcaa cactattatg gaaaagaatg gtctgtgcta
     2401 gagactgccc catttcatac tctggcgaga taaccgagtc agctccaatc ttttctagca
     2461 ctttcttagc ggtctgactt ttgaccttag caataacagt cggtaccccc aaactcttac
     2521 agtgcataac cgcaagcaca ctcgactcca gattttcacc tgtcgcgact acaacggtat
     2581 cgcaggtatc aatccctgct gatctcaata attcttcatc tgtgatgtca ccaatcactc
     2641 cacgcgccaa aactggctca aactgattga tgcgctctgc gtggtcatca atagcgataa
     2701 tattcatatc ctgcttggct agggcagcta ggacactgct cccaaaaatt cccaagccca
     2761 aaattccaat cgtacgatct gacatgctct ttcctttctt aaccaatact aatatctgct
     2821 ttcatatagt gaatcatatc tttcttttct ggatggtaat ctgccaagct aacaaacaag
     2881 gtcaagggac caattcgtcc cataaacata agtggcatga taacactgag agccaatttc
     2941 ccaaggtcag gagtcagatt tgccgttaca ccaactgtac taagagctga aatggtttca
     3001 aatatgaggt ggataaaggg aggattgcct ttggctgtta tccctagcag aatcaatcct
     3061 attaagaagc tcatcaaaaa gataataaag acactaaagg atttttgaac cgttcgcggc
     3121 gcaatcgttc gtctcgcaac attggcatga ggcaagccta gaagctcact tcgtgcaaag
     3181 accaagagga caaaaaatgt cgtaatcttg agtcccccag ctgttcctcc aggtgcccca
     3241 cctagaaaca tctgtaagat ataaatcaaa agagtcacag gatgagcctg agtataatct
     3301 atcgtagaaa agccagctgt tcgcatcgtc actgtttgaa aaaagctaac taaaacctta
     3361 tcggcaacag ggagattgcc aatcgttcca gcattgttcc actcaagaaa gagagtagtt
     3421 gccgttccaa ataacaacaa acctatagtc aacaatagta caagcttcgt atgaaagtgc
     3481 agacgtcctt ttttctttct tcctacatga ccagccaaat caaaccagac cataaaacca
     3541 aggccgcctg taataattaa gcctgcaatc accagattga ccagtaaatc ggtctgaaaa
     3601 gcaaataaac tggtgctccc taaattatca aaaccggcat tacagaaggc tgagatcgct
     3661 agaaaaatgg aactaaaaag accacgtccc cagccaagtt gaggaataag gcgaaaacta
     3721 agcaaaatag ctcccaagct ctcaaccaaa aaggtcgtga gaaaaataga atagacaaac
     3781 tttctcaaag atcgagtttc tccataacta aaactatcct gaatagttgc acggctacga
     3841 agactaagct tttgcttgct ctggatatag aaaaccccaa taaaggtcat gagccctaga
     3901 ccaccgatct gaatcaagag caaacagatt atttgacccc agatattata ggtgtgagct
     3961 actggaaggg ttgagagacc cgtcacacag actgcagaga cagcagtgaa aagatgatca
     4021 aaataagtcg ctcgtgagct ttcaacttgg acaaagggca agctcaaaag aagagagcct
     4081 agtaaaatga ccaaggcaaa actcaaaaaa atacgacggg ctggcgataa acgagataag
     4141 cctagctcta ttttttcctt agataatttg aataacatgt ttccattgta ccataaaaag
     4201 ttcgaaatga cttagatagg ataaggcaaa atctactaac accaagccta aaaaatgcca
     4261 cctttttatt gatagcttag gcaagaacaa tacttgatag atgtgactat aggtcaaaaa
     4321 agcaagacag gagcttttaa cgccagctac tctatatgct tttcaggaga aaatttctct
     4381 ttttgattat aggcaatgaa aaccaaggca tcgattagaa agctagtcac aggttgctca
     4441 aaacactgtt ttgaggttgc aaatagaact gacgaagtca gctcaaaaca ccgttttgag
     4501 gttgccggat aagactgacg aagtcagtaa catataccta cggcaaggtg aagctgacct
     4561 ggtttgaaga gattttcgaa gagtatcatt tcatctcact ctaaatctta tcgacataaa
     4621 acgcatagtg tcaaagtttt catcacctga tatgatacgt ttttctgatt ttaaagattt
     4681 ttcccaacct caacatgcct aggacaagag gaaggtgacc tcgtttaaac atatttttga
     4741 aaagtatgag ctagacattt tatgataaaa caaatccttt gccagtacaa ctaaaggtgg
     4801 tgaacacttg ctaaactcag ttctagcgcc ttattaaaag cttccgagcc ccagtcacga
     4861 ctgtcgtact ggtccaagtc cgctagagaa tctgctgtga acaacaattc accccagaga
     4921 accccacgca attgagctac tgccgcaaga gcggaacact ccatctccac aacagcacag
     4981 tcttcttcct tgcgataagc caccttttca gccgtttctc ggtaaaaacc atctgtggac
     5041 caggtcatga cttcttcata aggaatccct ctatcatcca aaactcgctc aatggcagca
     5101 atagcctctg gctgcatttc catataacga gaaggtgcca catagtggta actggctcct
     5161 tcatctcgca gagcgcgaac agggactaga aaggcatttt cctctatatc agctaggaca
     5221 ccacaggtcc cagtagagat aatctgctcc acaccatagc caatcaacca atccataaac
     5281 tgggctgctg gagctgaacc gacaggagcc tgaaccagac aaatctcctc gtccttgtag
     5341 ttcacgacat aaactggata ggtcttggtg gcagaaacaa attcgccaac acagttcgcc
     5401 cctacttccc tcgcatagcg gtcaatctcc tcacctaaaa atgcataaac acacttcttt
     5461 ggcaactgca agtccagccc ctcgtgattg ggcatgataa ccgcctgagg attgtcatca
     5521 aactctaaaa taggaatcgc atgtttctga atcatagcct gcctcctcta attttgtact
     5581 attgtatcaa aatctgaccg agtgtcaagg aggaagctga tgaactgagg agaaaaacat
     5641 cccacttacg agatgctttg aacttgctta atgaaacaga cttgctaaga gatagagtag
     5701 gtaaatgtga actgggtaaa aaatgatgaa acctacaaaa aagaaacaac cccagctgtt
     5761 cctttttact tctatttaac aggtatttcc ttaatcacgc gcgcaggatt gccagctagg
     5821 acaacgttgt cgccaaaaga tttggtaatt actgcccctg ctcctgcaac gacattattt
     5881 cccagtgtca ctccaggaag gacaatgacg ccaccaccag tccagaaatt atctccgatt
     5941 gtgataggct ttccgtactc gatacctgaa ttgcgttcct gtggatctag tggatggagg
     6001 ggtgtcaaaa actgacaatt aggaccaatc atagcattgt ccccgatacg aatgggacag
     6061 atatccagca tggtcaagtt ccaattagaa taaaaatttt cccctagatg gatgttgaca
     6121 ccgtagtcca ccatcaagcg agtgttgatg taaagatttt tcccggttga ggcaaaccaa
     6181 gtcttgatga tttcggctcc cttcaagggg ttctcttcct tgttaaaggc ggcctgtttt
     6241 tggcgagaag cctgagctaa ggctctcagt tctgggtctg atggacggta aaactctcct
     6301 gctatcattt tctggtattc gctagtcata ttattcttct ccttattccc tactatcata
     6361 tcaaattttc cacgtatctg actagaataa ggagcacttt ctacaattga tgctctacct
     6421 tcatcacata ccatctatct agctaaacga atacgggtca cgatgccatc tgagtctgtg
     6481 atttccaatt ggatcgatgt catccatttg attggtgcgt caacttcttg tgctcgttgg
     6541 gcaatcgtta acagttcttc tttatgtgcg acttcgatga catagtaggc taaacctggt
     6601 aggacttgtt tacgcggatc cagacctttt cctccccatt cgttgactgc taaatgatga
     6661 tggtagtccc cagctgcgat ccaactagca ctaggcacac tgaatttatc ctcgagccct
     6721 aacaccgttt gataaaactg tctggacttt cgactatcct tgacagaaag atgaatatgc
     6781 cccattctcg taccctctgc taggataaaa ggctctactc tttcccccaa ctcatagata
     6841 tcctgagccg caaggacttc agtcaccccg ataatacgtc catcttctcg aatatcccat
     6901 gtggaaactg gcttatctcg atagagttca atgccatttc cctccaagtc ctctaagtaa
     6961 agggcctcac tgtaaccgtg atctgcaccg ccaacaagag gaatctgtaa atccgtcagg
     7021 tgcttcaaga catccgccaa agcctttcgt gtcggcaaaa gaatagccag atggtaaaga
     7081 ccataatgtt cccttacttc tccaccctct tgtgcttgaa tcaagtgtac caaggctttt
     7141 cctccaagtc ccagaacgac ctctgtatcc gtttgagata ggatttctaa accaataatc
     7201 tgctgataaa aaactgtttg actgaccaaa tccttcacat ttaaaactgc ctctgctaaa
     7261 taaatgtggc tctggtattc atatgtcata aatatttcct cttttattgt aactttaatt
     7321 attacaacta ttatataact tccctcaaga aagtcaagcc agacaacttg aagcatccca
     7381 ctcagcaaaa aacaatccca gaaaaaatct tctaggattg ttttgtattt attttactag
     7441 actagcaaag ttatttctca aatcaacatt ttctgtggga ttgaaatcca ccttgtcacc
     7501 attggtcgtt gatgagttga agatgattcc tgcaatctgc attcaatcaa atctctacat
     7561 cgatccataa gaaaaaggag tcgatttgtc atctgactcc tcatccttac aagaaacact
     7621 actcaacaat gaaactctac aatcttattt atatatagaa tttctaaagt catctgtagt
     7681 gcgaagatat gcattataga ttttttgttt taataaatgc acccaacttg ctggggtatg
     7741 actcgttgca cgatctatat tagtaatatc tttcgcagcc gcttcattaa ttaattgttg
     7801 catttgtgca gccgttgtta tagttacttc ttttgtacta ttaggattac ctaattcgta
     7861 ttgaattgtt attggtttca gattatcttg tttatctata cgttgtttaa acattgcttt
     7921 tttgaaatca gcccatgatg agtactcacc attgaatact ttcttaaata ctaaatcatc
     7981 tgtcactaaa gcaacatctc ttccatgcca tgatgagaat gttttgcttc cgctggcaaa
     8041 tgcttctgct ccgtactgat tagaaacata aggtaggaat cctttgtgat aacctttttc
     8101 cgcaagtaat tcataagcta tttttctaaa cataatatct ccaggagcac ctttcgaatt
     8161 gcttagcgct gcgtatacag gagagaacat acttatagta tagtagccat ttcgtttata
     8221 ttctctatta tcatcatagc tacgtctatt tatgatgtcg ttgtcgatta atgagtttaa
     8281 cgatgttaag ttagctactt cttcatctgt taatggacgg actttatttc ctgcatgtgt
     8341 atctttatta tgtctagtat cacgaacgta ataattttct atttttctaa accatttttt
     8401 cttaacatca ttattttgag ctaatatcgc ttttgcttcc atcgcatcaa gtgtatacat
     8461 tacatcatat gatccatgca tataactttg aagcgcctca tccgaatcga aacgttccac
     8521 cggattatat gtatgcaatc tattcaaatc atctttttct gctttatata acgtatttaa
     8581 agctaaaata tgagaattta cactatctac agattgcagt aaaccaagtg cgtataactc
     8641 cgctcccaat ccttcacgtc taccatttcc ttcaaagtag attgcagaat cagaattatg
     8701 aaccatttca tgagtataaa ccgacgttcc ataagcatct aataatctat ctaacacaaa
     8761 gtgcgttaaa cttccattcg catacgctcc tgcactacta ttatactcat accatttccc
     8821 aacaggtccg aagaattctt taatagagcc gatgttttta tccgttaacc ttgcccaata
     8881 agttcttcct gtctcatctt ttacattgaa tccatcataa actatcacag aacggaaaag
     8941 tttttctttt gaagcactgt caagaagatt gtaccaaata tcataatggt ctctatgcct
     9001 tttcgctgct atatcaactc tatttcttac ataagttcgt aaagcatctc ctgaaagaat
     9061 aactccatcc acactatcac gatatctttc atacgaaccg aatgcaagtg tagacatatt
     9121 cgatgaaata tacacagatt cttcaggtaa agttagtagt ggtaataaca tactcttatg
     9181 cccccaactt ggtgctgata ttctatcgta aactcctaat gaatattttc tatcggctgt
     9241 tggtgattct tgtttttctc gtacttctgc aatatcagac ttcatttcga ctatatatgc
     9301 ctttgtattt tctttaaacc actcgttatt tgttttgttt ggtaagaaca gttttctata
     9361 cgcttctacg aagtcaaaga ctgaatcttc tccttttacc gatgcaagtt tattcgcata
     9421 taaacctaca gtatttgaag ctctcaggtt atctagtcca ctatttccta atgcgacaat
     9481 agtatccaac gttgaagtct cattatttcc gttaaagtca aacttgtacg tagataaatc
     9541 ttttgtattc attttaccat aattaatatc gtaccaacgg ttcatataag taagacctag
     9601 cataaatgct tctttgttat ttttgatttt ctcacttacg tattcaacta caccgtctcc
     9661 tgtagtattg attgatttat ccatcgctaa tacttttctt aggtgttctg ctatattagc
     9721 tttaacttct tcaaattgtc tatctaagta taggttatct aaggctgcat cattcgctgc
     9781 tcctagtact tttttagtag cttctgagtt aagtgttaca ttttgcaagt cgcttagtac
     9841 gttattcgtt atcgctgtat agtctgaaac aaatgcttct ggtgtgaata tatattcttt
     9901 tcctgtaaca ttgtattcga ttacttgact gtttatgaag ttttctttga atgttacatc
     9961 taggtattct actgtattat ctttgaaatg taacataact ttatttattg aatttttctt
    10021 attattaata tccgttacta cttcatcatc tttcatcggc acaacatcta acaattctgt
    10081 agtgtaaagt ttatctgttg tcgctacttt gttaccatag tgaactacta ggtctttatt
    10141 gtagaatggc atcagttttt ctatgttgct ataagctact tttctttcag cttctgcttt
    10201 atttagtctt gtataatcaa cttctcttag attacgtttt aaatcttgcc cagtatcatc
    10261 aagagttact gtgattccat aatcagcaac tttcgcgtct atttggtctt ttgataattt
    10321 tgtagcgaat ctgtctgctt ttctattatc aactgatgta cttgcatttt tcacatctgc
    10381 tgctgcgtat tgatcaccgg tgataacata accatctcca acatctacgt tactcacaac
    10441 attatttact cgaccgtttt gccacgtaga tcctactatt cctccgactc tagaatatcc
    10501 ttgaccattt cgtatttctc cagtagcaac cgaattagat atcaatgcac cattttctaa
    10561 tctacctact atccctccag ctgtttggtt attattgcgt gcattagtag agattaaggc
    10621 atctacccta actttattaa ctctcgaact atttcctgtt atattaccta ctattcctcc
    10681 agtatcattt ttattactgt cctggtgatt tgcgataagt ttccctgtaa acgagctgtt
    10741 ttctatcact gtatttgttg cgctcgctac taatcccgca acagatttcg cacctgagat
    10801 ttttccttct actgcaacat tattaatatt cgcgctattc gctgccttcg ccagcgctgc
    10861 gacattttct ttactatcag cagaaacagt tttaatatcc aaatctctaa ctgtagcacc
    10921 atttaatgta tcaaataatg gttttttcaa atcataaatg gcatacgatt ttgttccatc
    10981 agaaccgatc aagctccctg taaatgcacc tgtgagataa cttgtctgct tatcgcctaa
    11041 gctcacctca tctgcggtca tatctgaagc caatgtatag acaccagaca gattgctttg
    11101 catggctgtt accagctgtt taaaggatgt gtaaactcct ggttgctctg ctttagattt
    11161 agctacagta aatgtgtaat catctttgta accgtctgta ccttcttcga caagttgatc
    11221 aacggctacc gtcactttat acgttccatc cgtattttct gtaatagatt ttacaggtag
    11281 gtacatttct ttgaagcgat ctgatttcac ttttacaaag tatttagccg tatcagtcgg
    11341 cgcttcactt agacttaaat atctacgata acgatcattt tctttaccgt ataattctac
    11401 tgaatcaata tctttaatct ctattttctt atactctaat tggaaatctt gagttgatgt
    11461 ttcagtattt tcctcattat tttcacccaa attataagtt aggtgtgttt taactgtata
    11521 cggtgtgtag taatctaaac ctgatattac ttgctctttg gcaggatttt ctatatccac
    11581 ctctttaact agcttgtctc catggaaaac ttgcgttttt gcagaaacat atgctgaggt
    11641 agtgtctatt aagttataac ttacagttat agattttttg ttctcaactt ttgttaagtt
    11701 tgtaatttct actgtaggtt taactttcac aagtgttcct actttaacga cttcgttgac
    11761 cggagctact tcagttcgtg acacttcttt agtttctacg acattaccat ttacgatgta
    11821 gtcttcatat tgaattgtac gtgtccctgc ttgcccttgt cgttcaatct tacgacgatt
    11881 tttcagaagt gttggatcct gaatttcttc tgttgtataa ggaatattct ggatttccgt
    11941 tctatttcgt gtagtgacct ctaaagccgg aagttcttct tctactgccg cttcgccctc
    12001 atgttcgggt tcttgtgtgc cttttgtcgc taccggttcc gtgtactcta gagtctcttc
    12061 gcggactgta gctttgccct catgacctgg ctcttgcgtg cctttcgttg ctaacggttc
    12121 tgtgtaagct ggttcttcct cgcgaactgc agcttcacct tcatgtccgg gtccttgtgt
    12181 acctttggtt tctagtggct tagtgtagac tggtaagtct tcacggactg cggcttcacc
    12241 ctcatgaccg ggttcttgcg tgcctttggt ttccaatgga agtttatact ctggtcgatc
    12301 atttgtcgtc gccaatactt cctcttccgc actctgcacc gttgtagcag acacaggatt
    12361 tccttgttct ggcactgttg gtcgaactac agattctcct ttgtgatccg gattctgctc
    12421 aacttgactt gcgaatgaat cattagaaga tagattgtct gctgctatag aagatttttc
    12481 tgaaagtcca tcatcccctg atgcttcacc ttgtaaacta accttcccct gtccttggtt
    12541 ccattctaaa tcagctgaat gaactacatc tgatgttttt gtagagtttg gttgactatc
    12601 tctttgagca gagtatttcc catcaactgt ccttgaaagc tctgtattat cctgtttctt
    12661 agttttgata taaccaatat attgataacc ttcgattttc agaggctctg gtaaatgttc
    12721 cccgactccg atagaaagct gactattata ggcagataaa atctggctgg tcaacccaaa
    12781 agcactggcc ggcaacaatt gaactcccat gctagtcaac agcagaaaat gaacaagtcg
    12841 tttctttcca ttttcccttt tagaaaccaa taaaactaac aagctcaatc ctgcagtaaa
    12901 aaggaaagta ccaacagttg ggttaaaacc tgtattcggt aaaaactgtt gagatctata
    12961 aaccaagtaa taagtatcat ctgattcttc agccagttgt ggaagggcct ctaccagcaa
    13021 ggatttttcc tcgtctgtca atttaccctc ggtcatatac ttataatgaa ttggactttc
    13081 catattatcg gccgataccg attgaatccc ttgactcatc aaaaagaaac tagatacagt
    13141 aactgatacc aatcccactg acaactttct catcgagaaa ctatggcgtt tctcaccaat
    13201 gctttttcgg ctcatattaa cctcgctttt tctatattct ttatatcaat ctatatttta
    13261 tagcataaaa gtataaacat aaaaataata tagtatggaa ttttctgagt acttttcaac
    13321 aattacttat cattatacaa taaatcgata gaaatttcta ttgaaatata cttaaaaata
    13381 ttctgcatac tataaaaaat acagtaaatt ataacgataa aaaacagaga taacatctca
    13441 acaagtaagt gtggacttca gactaattac agcatttttt aaattagatt accgcttatg
    13501 cttttaatgg gctataaaat attagcttac aatagaaaag tctgaaattg aggcgacaag
    13561 aatccaatct ttataaatca aaagaggcca atgttcgcca tcaacctctt tcttatcgtt
    13621 cattcttttt gctaagtaag atgaatgctt tacctatggc ctgagcctag tttacccatt
    13681 caccattacc atttacacgg taaccatctg gtgttgttgt gctcacagcc aaagctcctg
    13741 aaccgtaggc atagtaccag tgtggtccga ctttaaacca acctgtcttc atgatacctg
    13801 cttcgtcaag gtaataccaa gtgccatttt ccttgtacca gcctgtcttc atagctcctg
    13861 agccgtcaaa gtagaaccat ctgctaccat ctgttcccca gcctgtaaac atagcacctg
    13921 aattgctcaa gtaataccag ctaccagaaa attttacaaa tccagtctgc atgacaccta
    13981 agtcattcaa atagtaccat ttaccatctg ttttcaccca acctgtcttc acatcaccag
    14041 actcatagaa ataccattta ccattagatt ccacccaacc agactgatca gacgtagacg
    14101 ctctaaaatc tttcagtggt ttttctacaa ccgtagcttt agttttaact gttgaaaggt
    14161 ttgaggcagt cacttctgta cactgaacag cagaaccata tctaccagcc aattcctctg
    14221 aagaagcatc atcacctaga gtttgagcaa ttgtaatagc tttgtattga ttgttaggat
    14281 ttttaaaagc tgccatcccc atgtgtgtaa atttagggtt aattagcgac tcatagtgac
    14341 cagatttccc gttgtctgaa ccactatttt tgtctccaca taatctgctt tttcagcacg
    14401 ccattgttca atagctttta gaaaaccgtc atgattccat gccaaatttt ctcccattat
    14461 actattagaa gttggaaagg cactccaaag atctttgcta gaaagacggg tatgatccat
    14521 ggttatagat gcttctgtag ctctggcaaa agctgccttt tctaggtcag ttgaccattt
    14581 gatagggaca tacttatcta ccaaaccttc gtcagccgct tctttacaga tagcattgat
    14641 ggcatccaaa atagattgtt gatgaggagc cacaaattct cctttgattc caaccaaaac
    14701 attgccacta gaaggtttta gcacctctga ggtcaataca ttttcatttt taccatcagt
    14761 agtttgtgtt gccacaagtc tgtcgttcgc aaaaacatca tttgtcaaaa ctgctccagc
    14821 caaggtcaaa gccaaggcac tagcaaatac aattttcttc gtttctttct tactccttat
    14881 tgtagtttga atattatatt aacagatagc tttcaacgct tagttatatc atattttcct
    14941 actatttgtc tctgcgtacc tattcctagc tcccaaaagt aggcgcaatg actcttttac
    15001 ttcacaacaa gttcataacg tgtcttactg gtgaaggttt gaccagcttt aagaatgact
    15061 tggcctttaa ggtcactgtg aatggcatct ggtaaagctt gcgcttcaag agcaatccca
    15121 ttgtgctgta gcattggctg acctcctatg atgacacttt catccacaaa gtttgctgtg
    15181 tagaccacaa agcaaggagc ttctgtcttg aaaagcagga agcgacctga attttggtca
    15241 taaaggaatc cagcattgtc atggcctgca ggaagggcaa atggatgatc caaacctgat
    15301 gccagctgga tttgctcatc ttcttctgca aagatatcct tcaacaaggt accattgtag
    15361 acgtgtttga ccacatcacg gttggcttct ggagttttgg caggaacacc gtcaggagcg
    15421 attgagtaaa tgccctctgt gtttagttgg aagacatgac ggtcaatcgt ctgcgtgaaa
    15481 tcaccagaca agttgaaata gctgtggttg gttggattga ccagcgtatc ctgatcggtc
    15541 gttaccttgt agctgatttc ataggcacca gtttcttcca agtgataact gatccaaatc
    15601 ttgagatttc cagggaaccc tcctgtccca tctgtacgct ctgtgtagag agtcaagcca
    15661 tgatcgctta cttcttcaac ttcaaacaag ctggaatccc aaccagttga accactgtga
    15721 ttacagttgc tagcattatt aacctcaagg tcataggtct taccattgag ctcaaaggtc
    15781 gcacctgcaa tacgacccgc tacaggacct acacttgctc catgcttggg actattgcct
    15841 acataactat caaagtcatc aaatcccaag ataacattgg caaaatttcc agccttgtca
    15901 ggtgcgacat agcgcaagat agtcgcacca taagtcataa cctcaagttg gtagccaccg
    15961 tctgtctcaa atcgataggc caagacatcc tcaccctcaa catttccaaa tacacgctct
    16021 gtgtatgctt tcattctgtt ctccttttac tatttctctc aagcaaacaa accatagaaa
    16081 gcgtactgac aatctatggt ttatctgata atttacaaat cctcttgtca agaattcata
    16141 aacactgtct tacttttgat attcgtgaat tatgacacct tgtactacac ggtttactgt
    16201 acctgtagga gacggtgtat ctggtttatt ttctaccttg agtgaagtca ataaagcaaa
    16261 gagttgggca taaacgatgt aagggaagac acggtaaata tcattcaaga caccgccaca
    16321 accaagggcc acttctttga cattttcaag accaaaagct tgatcactca aaagcacaac
    16381 acgacgagca atctggtcac cagcaacttc acgaaccaag tccaagtcgt acttacgagt
    16441 gtagtctgtc gttgtaccaa agaccaaaac aactgtattg tcgttgataa gagattttgg
    16501 accgtgacgg aagccaactg ggctttcata catggtcgca acttgaccag cagttaattc
    16561 caaaatcttg agctgagctt catgagcaag tccaaagaaa ggaccagcgc ctagatagat
    16621 gacacggtta aagtctaaat caacgagctc tttgacatct tctgccttgt ctaaaacttt
    16681 acgggcaaga ctagatacaa cttcaaaacg ttcagactta acagcaaatt ctgtaggatc
    16741 aaagaccaag agagttgtca acatcataga cgtaaagcta gaagtcatgg caaatccagc
    16801 atcattagag acagctggtt gcaagagcaa gagattacga tcatcaccgt gagcttgaag
    16861 agccaattta ccatctgctg cacaagtaat cgtcacttga taaagctcat ccaccaagga
    16921 tttggccaaa tcaacagtcg ccaaactttc aggcgaattc ccactacgag caaaagacac
    16981 aaggacagtt gccacatctt ttttcaaata ggttgctgga ttggcaacga tatctgttgt
    17041 cgcaatagca ttgaaattcc atttgcgttc gtcatagact tccttaaaat aaggtagcaa
    17101 ggtatctccc acataagcag aagtcccagc acctgtcaag ataaccttaa tatagtcatg
    17161 tttatcagcg atttcttgta ggaaggctgc aatttcttca cgttttgctt gataaaattc
    17221 aaaagcttct ctccatacat caggctgttg gtagatttca cgcgtagtga tttctgcacc
    17281 caattcgagc aagtcttctt ttgtataatg tagcatttag ttcctctttt tctattaaat
    17341 atgggaatgg gagtaagcta cattcccatg tatattctat ataaaatgct ctaacgattt
    17401 tcacgttgtc acatgaattg aatcgagttc caactaaaat ctgtaggaaa ctagccgcag
    17461 gctgctcaaa gcactgcttt gaggttgccg gataagactg acgaagtcag ctcaaaacac
    17521 cgttttgagg ttgccggata agactgacga agtcagtaac atatacctac ggcaaggcga
    17581 cgttgacgta gtttgaagag attttcgaag agtattattc atcttcgtca tcatcgaagc
    17641 ccgctaacag gtcattaact ttcacaatgc tttctgcagc acggctcttg tagtccgcat
    17701 ctgcgcctgt aaggctcgca ttgataaatt caatcaccat tggaagattc atccctgcgt
    17761 aaaggtcaat atcacgacct tccatgatca agcgactcac cacattacaa ggtgtcccac
    17821 cgagaagatc cgcaaagact aggaaatcat ccaatccttc aataacagct tcaaatttag
    17881 cagtaaattc ttctgggcca tcttctggaa gaagagctac tgtgtaaatg ttgtcttgtg
    17941 ggcccataat catttctgtg ctacctctaa gctcctcaca gaagcgacca tggctcacca
    18001 aaattaatga tttagtcata aggaattaca ttccaagtgc aaagtgacca agggcagaaa
    18061 gagccaaagc aagtacgata ataataccga tagctttagt agagttcata cctttcttac
    18121 caagcaacca gaagataaag gcagtaaaga ttgctggaag caaacgtggg aagatttggt
    18181 tcaagatgtc ttggaaatca atcatctttt caccgattgg caacttgtaa gaaatttcaa
    18241 agttaatcac tgttgctaca agagcaccca tcatgaagac accaagtaca gatgcagcgt
    18301 caatcaaagc tgtcaaggta ctttgcatgt tgttgataag gttaacccct tctttgtaag
    18361 caaattccaa ctgtttccaa cggaagatgt catacgctac tgcaactgca atccaaagga
    18421 agatccccca aggttggcca gcgatagcca tagttgctgc gactgacccc atgatagcag
    18481 gtacaagtga accaaagatt gtatccccaa gaggagcgaa tggtcccatc aaacctgtct
    18541 tgataccgtt aacggcgtct tttgaaccta caccatcttt ttcttccatg gcaaggtcaa
    18601 aaccagcgat aatggtatgg aagaatggtg aagtattgaa gaattgagta tgaactttca
    18661 tcatttcttt caattcagga gttccatcac catacatttt acgcaactga ggcaagatca
    18721 tgtaaaggta accagaagct tgcatacgtt cgtagttcca acctaattgg aaagtaaaca
    18781 agctacgttt gttgatttga ttaaaatctt cttttgtaag tttgtaatta gaattcgtca
    18841 tcttcgattt ccccactttc tgatggtgta gaaggtgctg ctacagctac tttttggcta
    18901 tttttgaagt gaagcactgc aaggaaaata cctacgatag aaataccaat catagacaaa
    18961 cctttgaagt tgttcacgaa accaattttt tcagcaactt cagcaggaag agtacctacg
    19021 ataccagcaa cagcgccacc aagacctgtt acatatgagt aaagaacagt caacatagct
    19081 gtcaaaccaa atcccatagc aaggtagtga aggttacgtt taactggaag gtaacgaagc
    19141 aagattgcaa atccaagacc tggaagcata cgtcctgcaa gtgtcaagcc atctgcaacc
    19201 catttgtagg cttcaacgaa gtctactact gattgtacaa aggcaccacc aaaagcaagg
    19261 gcaaagaaga ctggaagggc acgagataga gcccacggaa tcgcaccaag caagtagttg
    19321 cgttcaatac ctttatagtc aaagcgttcg attgcagcat ccacacggtg agcgaagaag
    19381 gtagtagtca tacgaccaag aacgtcgaag taagtcaaga gagctgctac tggtacagcg
    19441 attgtagtaa tggcaagcgg tgcatcaatt ccttgtgaaa cagagaaggc tgtcgcaaga
    19501 accgcaccag aagttgcgtc gatacgagaa gcaccaccga aggtaccaac cccaagaacg
    19561 aacagttgca agttaccacc gataagtaaa ccagtagtca catctcccat gattaaacca
    19621 gtaatgaaac cagcaaatac aggggaacct gcagatgaaa cgatcgtcaa ctcatcacag
    19681 atttgataag ctgagtacaa agtgagaagt aaaatttgcc accattgtat cataacaatg
    19741 acctcctaaa aatttttttc ctattttaat aagctcaaaa agtctgaaat tggatcattt
    19801 ggaaccatct gagcagtaag tttaacacct ttttctgcca gtttgtggaa gtcttccaca
    19861 tccttgtcta ctacgttgat agaacgtgta atagaacgag tttctggtgt ttgagacata
    19921 ttcccaacat taagggtttc aagtggtaca cctgcttcta ccaaaccaag gaagcggtct
    19981 ggtttacgag ccacgataaa gagacgttgg ctatcgtatt tgccagcaag aatattggct
    20041 gcagctttct caactggcaa aatactcaat ttcacacctg gtggtgtcgc aagtttcaaa
    20101 ccactctttt caatatcgtt gttgacaact tcgtcgtcta caaccataat gcgtgaaaca
    20161 tttagttttc cagcccaaag attggctact tgtccgtgga tcaaacgtcc atcaatacgg
    20221 catcctacaa ttgtcataag ttttccccct ttatatgttt tagtgtaggt ttacgagtta
    20281 aatgaatctc ttctttatat tgaccttctg tttcaaagat aatgatgcgg ttggcacctt
    20341 ccttgagata gctatgaggg atataaagtg agagagttgg gccaacgttc caaaaacgtc
    20401 ctagattctg cccattgaca aaggcaaccc ccttaccaaa ctcagacaag tctaggtaag
    20461 tatcttttgg ctcttcgact gtaaagtcat aagcgtaaaa ggctggttgt ccttgagtcc
    20521 atccttttga aaaatcaatt ttctcaggat tgtctagtgg gagtggatag tgtttccagt
    20581 ttagtaagaa atgcagatcc ttacagaccc ctgtccgaat tcccttacgt tgcgtatccg
    20641 ctaagaactt atgcccatag ttgacacgcc ccatattttc tatcaagata tctaacctag
    20701 atagcccttt ctttttacct tgataaaaaa tatcttcccc aatctctgtc tgatattgag
    20761 ttttaaccca ctgaccatcg acatacagct gggccctatc tcgaccatca atgatacgaa
    20821 gtctttcttc ttctgcatcc cagtttgttt ctgttcgata aagtaggtag ccataacttt
    20881 gtcccagctc ctccatcttt tgaggataga gactttctac aggacttgac aagctatcta
    20941 aggtttcaaa caaagaaact ttttcaacta gtggaatagc atccaactcc atactctctt
    21001 tgtagagtgg ttccaactgc ggatactctg aaaaatgtgt tgccatcatc ttcttgactg
    21061 caagatattt agcagttgga tttccttctt catccagaag ggcatcgtaa tcataagacg
    21121 taacttgtgg caggtccaaa gttcctcgag ctgagcaacc attcatgaaa ccaaagtttg
    21181 taccaccgtg gaacatgtaa agattgatag agccttgttc caaaacctct cgaactgcat
    21241 ctgccaattc cttaggatcc cgtgtgataa tcggttcttt ccagcgattg aaccaaccat
    21301 cccagaactc catacacatg agtggccatt tcttaccatg ttcatcaaag aattcctgca
    21361 tctgcgaaaa gttgtaaggt gccttagaac caaagtttcc tgttacaaag aggtcctctt
    21421 caattaaggt tccagctttc agagtagctc gccatggacc atctgatgta aagaggggac
    21481 aggttacgcc acactcttcc attagctgtc gaatcgctct caggtaagcc ttatcttctc
    21541 cgtaagaacc atactcattt tcaacctgca tcatgagaat attgccacca ttgtccaaca
    21601 aacgaggcac cagtcttggc aataactgat catagtagcg accaactgcc tcgatatatg
    21661 ctgggtcgga tgagcgaatt cgcatgttct tggtcaagag ccaagctggt aagccaccga
    21721 attcccattc cgcacagata aatggagacg gacgcacaat tgcgtagaga cccaaatcct
    21781 gcgctatttg gagaaatttc tctaaatcca gatcaccttc aaaatgaaac tcaccttcac
    21841 gaggctcgtg taaattccaa gcaacataag tctctaccgt attaaaacca agagccttca
    21901 agttatagag cgaatgatac caatcctctg gaggaaccct aaaataatga atggcaccag
    21961 ataaaatctt aaatgatttt ccatcgagat agaaatcatc tcgtatctca aatcgtgtca
    22021 taaaactacc ttccgaccgc gaaagtttgc gctttcattt gttgttatat taattataaa
    22081 ccaatctgtt tgaattgtca atagaaattg tcgggatatt tactgcattt ttactattat
    22141 acacacattc aattagtttc aaacattttt agacccattt caaacgctct ttaaattcgg
    22201 aatataggac ctaaaataat tttgtataga atacattgat aaaatatgag caatccctat
    22261 atcgcttaat ttttcagact attattctaa ttttttattc aaaagactac tatttttttc
    22321 gcttttatgg taaaattttc tatggagaag gaaaatagag gtaaaaatat ggcaattccc
    22381 aaatatcaat acataaaaga cgaattaaaa aataaaatca tctctgggca gtttagcagt
    22441 ggagataaat tttacactga agcagaactc atttcaatgt acgatgtcag ctctatcaca
    22501 gttgttcgcg ccttaaacga ccttgccaaa gatggctata ttgtccgcca acaaggaaaa
    22561 ggaaccttcg tttcacgtgc acgcaaacac aagcttgtag agttttcaga tattgaactc
    22621 ttcaatgcta aagatgataa ggtaactgtt ctttcaattg aacgcggaaa caagcttgtt
    22681 tacttggaaa aattgggtct acgtggcgat caattctact acaagattga acgtatccgc
    22741 gaatcaaacg gtgtcgttta tatctaccat acatcctaca tcccagaaca atatatcaac
    22801 gcaaattatc caaaccttga atactatagt tctatctata atcgtttcaa gttagactac
    22861 cacattcaca tgaatgatga acactttgaa gaaatcaacg aaatagtctt tccaactcca
    22921 gaacatgcgg cttcagtcct cggagtcgat gaacaattcc ctactgtcct acaaactaaa
    22981 atcaccaaac tcgaatcaac tggccaagtt ttagaataca gtgaaactta caaacgatct
    23041 gattactaca agattaaatt tatctcatgt gaccgtgatc actaagaaga aagcctgagc
    23101 taatcgcttg ggctttttct atgcttatta taccacacca aaaaggtagc gcttacctat
    23161 ttttggaaaa ttctataatt tgtggaatat tttatagaaa tagaaccaaa aattatacta
    23221 gatatatata cgtatttttt ccgaccaatc tgaaatctta agtttaaatt ttatattttt
    23281 ttatacttat tatagttttt tgtcatctcc tcttgactct cgttaatata agaaataaaa
    23341 taaaggcgtt gatttatata atcgctatca atataacaat gcaatcagga ggttttgcaa
    23401 tgaaacatga aaaacaacag cgtttttcta ttcgtaaata cgctgtagga gcagcttctg
    23461 ttctaattgg atttgccttc caagcacaga ctgttgcagc cgatggagtt actcctacta
    23521 ctacagaaaa ccaaccgacc atccatacgg tttctgattc ccctcaatca tccgaaaatc
    23581 ggactgagga aacacctaaa gcagtgcttc aaccagaagc tccaaaaact gtagaaacag
    23641 aaactccagc tactgataag gtagctagtc ttccaaaaac agaagaaaaa ccacaagagg
    23701 aagttagttc aactcctagt gataaagcag aagtggtaac tccaacttct gctgaaaaag
    23761 aaactgctaa taaaaaggca gaagaagcta gccctaaaaa ggaagaagcg aaagaggttg
    23821 attctaaaga gtcaaataca gacaagactg acaaggataa accagctaaa aaagatgaag
    23881 cgaaagcaga ggctgacaaa ccggaaacag aggcaggaaa ggaacgtgct gcaactgtaa
    23941 atgaaaaact agcgaaaaag aaaattgttt ctattgatgc tggacgtaaa tatttctcac
    24001 cagaacaact caaggaaatc atcgataaag cgaaacatta tggctacact gatttacacc
    24061 tattagtcgg aaatgatgga ctccgtttca tgttggacga tatgagcatc acagctaacg
    24121 gcaagaccta tgccagtgac gatgtcaaac gcgccattga aaaaggtaca aatgattatt
    24181 acaacgatcc aaacggcaat cacttaacag aaagtcaaat gacagatctg attaactatg
    24241 ccaaagataa aggtatcggt ctcattccga cagtaaatag tcctggacac atggatgcga
    24301 ttctcaatgc catgaaagaa ttgggaatcc aaaaccctaa ctttagctat tttgggaaga
    24361 aatcagcccg tactgtcgat cttgacaacg aacaagctgt cgcttttaca aaagccctta
    24421 tcgacaagta tgctgcttat ttcgcgaaaa agactgaaat cttcaacatc ggacttgatg
    24481 aatatgccaa tgatgcgaca gatgctaaag gttggagtgt gcttcaagct gataaatact
    24541 atccaaacga aggctaccct gtaaaaggct atgaaaaatt tattgcctac gccaatgacc
    24601 tcgctcgtat tgtaaaatcg cacggtctca aaccaatggc ttttaacgac ggtatctact
    24661 acaatagcga cacaagcttt ggtagttttg acaaagacat catcgtttct atgtggactg
    24721 gtggttgggg aggctacgat gtcgcttctt ctaaactact agctgaaaaa ggtcaccaaa
    24781 tccttaatac caatgatgct tggtactacg ttcttggacg aaacgctgat ggccaaggct
    24841 ggtacaatct cgatcagggg ctcaatggta ttaaaaacac accaatcact tctgtaccaa
    24901 aaacagaagg agctgatatc ccaatcatcg gtggtatggt agctgcttgg gctgacactc
    24961 catctgcacg ttattcacca tcacgcctct tcaaactcat gcgtcatttt gcaaatgcca
    25021 acgctgaata cttcgcagct gattatgaat ctgcagagca agcacttaac gaggtaccaa
    25081 aagacctgaa ccgttatact gcagaaagcg tcacggccgt aaaagaagct gaaaaagcta
    25141 ttcgctctct cgatagcaac cttagccgtg cccaacaaga tacgattgat caagccattg
    25201 ctaaacttca agaaactgtc aacaacttga ccctcacgcc tgaagctcta aaagaagaag
    25261 aagctaaacg tgaggttgaa aaacttgcca aaaacaaggt aatctcaatc gatgctggac
    25321 gcaaatactt tactctgaac cagctcaaac gcatcgtaga caaggccagt gagctcggat
    25381 attctgatgt ccatctcctt ctaggaaatg acggacttcg ctttctactc gatgatatga
    25441 ccattactgc caacggaaaa acctatgcta gtgatgacgt taaaaaagct attatcgaag
    25501 gaactaaagc ttactacgac gatccaaacg gtactgcact aacacaggca gaagtaacag
    25561 agctaattga atacgctaaa tctaaggaca tcggtctcat cccagctatt aacagtccag
    25621 gtcacatgga tgctatgctg gttgccatgg aaaaattagg tattaaaaat cctcaagccc
    25681 actttgataa agtttcaaaa acaactatgg acttgaaaaa cgaagaagcg atgaactttg
    25741 taaaagccct catcggtaaa tacatggact tctttgcagg taaaacaaag attttcaact
    25801 ttggtactga cgaatacgcc aacgatgcga ctagtgccca aggctggtac tacctcaagt
    25861 ggtatcaact ctatggcaaa tttgccgaat atgccaacac cctcgcagct atggccaaag
    25921 aaagagggct tcaaccaatg gccttcaacg atggcttcta ctatgaagac aaggacgatg
    25981 ttcagtttga caaagatgtc ttgatttctt actggtctaa aggctggtgg ggatataacc
    26041 tcgcatcacc tcaataccta gcaagcaaag gctataaatt cttgaatacc aacggtgact
    26101 ggtactacat tcttggtcaa aaaccagaag atggtggtgg tttcctcaag aaagctattg
    26161 agaatactgg aaaaacacca ttcaatcaac tagcttctac caaatatcct gaagtagatc
    26221 ttccaacagt cggaagtatg ctttcaatct gggcagatag accaagcgct gagtacaagg
    26281 aagaggaaat ctttgaactc atgactgcct ttgcagacca caacaaagac tacttccgtg
    26341 ctaattataa tgctctccgc gaagaattag ctaaaattcc tacaaactta gaaggatata
    26401 gtaaagaaag tcttgaggcc cttgacgcag ctaaaacagc tctaaattac aacctcaacc
    26461 gtaataaaca agctgagctt gacacacttg tagccaacct aaaagccgct cttcaaggcc
    26521 tcaaaccagc tgcaactcat tcaggaagcc tggatgaaaa tgaagtggct gccaatgttg
    26581 aaaccagacc agaactcatc acaagaactg aagaaattcc atttgaagtt atcaagaaag
    26641 aaaatcctaa cctcccagct ggtcaggaaa atattatcac agcaggagtc aaaggtgaac
    26701 gaactcatta catctctgta ctcactgaaa atggaaaaac aacagaaaca gtccttgata
    26761 gccaggtaac caaagaagtt ataaaccaag tggttgaagt tggcgctcct gtaactcaca
    26821 agggtgatga aagtggtctt gcaccaacta ctgaggtaaa acctagactg gatatccaaa
    26881 aagaagaaat tccatttacc acagtgactc gtgaaaatcc actcttactc aaaggaaaaa
    26941 cacaagtcat tactaagggc gtcaatggac atcgtagcaa cttctactct gtgagcactt
    27001 ctgccgatgg taaggaagtg aaaacacttg taaatagtgt cgtagcacag gaagccgtta
    27061 ctcaaatagt cgaagtcgga actatggtaa cacatgtagg cgatgaaaac ggacaagccg
    27121 ctattgctga agaaaaacca aaactagaaa tcccaagcca accagctcca tcaactgctc
    27181 ccgctgagga aagcaaagct cttcctcaag atccagctcc tgtggtaaca gagaaaaaac
    27241 ttcctgaaac aggaactcac gattctgcag gactagtagt cgcaggactc atgtccacac
    27301 tagcagccta tggactcact aaaagaaaag aagactaagt cttttcgata aaaaataaac
    27361 agcgagattg aagctcgctg tttatttttt aattaatcac ctagtccaag acgttcaaag
    27421 atatcatcca ctcgtttggt gtaataaact gggttgaaga tttcatcgat ttcttcttga
    27481 gtgagacgtg atgttacttc tgaatctgcc tcaagaagtg gtttaaagtc tacttggttg
    27541 tcccaagagt aggctgtttt tggttgcacc aagtcatagg cttgctcacg ggtcatgcct
    27601 ttttcaatca atgtcaacat agcccgttgg ctaaagataa gaccaaaagt cgagttcatg
    27661 tttcggatca tattttctgg gaagactgtc aagttcttga cgatatttcc aaaacggttg
    27721 agcatgtagt caatcaaaat ggtcgtatct ggtgtgatga tacgctcagc tgatgagtga
    27781 gaaatatcgc gttcgtgcca gagagcgacg ttttcataag ccgtaatcat gtgaccacga
    27841 atgacacgcg ccagaccagt catattttca gaaccgattg ggttgcgttt gtgaggcatt
    27901 gctgaagacc ctttttgccc tttagcaaag aactcttcta cttcgcgttg ctcagatttt
    27961 tgtagaccac gaatctcagt cgccatacgt tcgattgaag tcgcaatgct ggcaagaacc
    28021 gcaaagtact cagcgtgaag gtcacgagga aggacttgtg tagagatttc ttgggcacgg
    28081 atgccaagtt tatcgcagac atactcctct acaaatggtg ggatattggc aaagttccca
    28141 accgcaccag aaatcttacc agcttctaca ccagcagctg catgctcgaa acgctcgata
    28201 ttgcgtttca tttcgctgta ccaagttgcc aatttaagac caaaggttgt cggctcagcg
    28261 tgcacaccat gagtacgccc catcatgatg gtgaacttgt gctccttggc cttgtcagcg
    28321 atgatattag tgaagttttc aaggtcacga cggatgatgt cgttggcctg cttgtagagg
    28381 taaccataag cagtatccac cacgtcggta gaagttaacc catagtgaac ccacttgcgc
    28441 tcttcaccaa gagtctcaga aaccgcacgc gtgaaagcca ccacatcgtg gcgcgtctcc
    28501 tgctcaattt ccaaaatacg gtcgatgtca aagtccgcct tcttgcgaat caaagccaca
    28561 tcttccttag ggatttcccc caactcagcc catgcctcgt cagagaggat ttccacctca
    28621 agccaagcac ggtatttatt ttcttcactc caaatattcg ccatctcagg gcgagagtaa
    28681 cggttgatca tgtgttaatt tttcctttct tcttaagctg ttgggaataa gctatctagg
    28741 taaaccaaga tttctactca aactcctctt tttcaataaa cacagacggg taatgatcta
    28801 gtgtattcaa atccgtatcc agcggtacat catagatagc taaataattt tcggggtatt
    28861 gtgcagctag ttcttcatat ttagcttgta ccttatcttg gtcagcactg gcatacaaca
    28921 cttcaacaac tgctccagtc ttatcctttt caataaatgt attaacgata atttgtatca
    28981 tagacttgtc ctaaaaatca atcccttccc caagctcatc cacactatcc ggtacatcac
    29041 taaacaaggt cacatgtccc atcttgcggt tgtgcttcgc ttctatttta ccatacatgt
    29101 ggaggtgggc gcttgaattt tctgtcacat attgctcagc agcctcgaca tgctgaccga
    29161 ggacattaag cataacggct ggcgcatgga gtttgatgac tggtaatggt gctccgagaa
    29221 caccgagaat atgggtgtca aactgagaga aatcacaggc ttcaatagaa tagtgcccag
    29281 agttatgtgg tcgtggggcg atttcattga caataacgtc atcatcagtc gcaaacattt
    29341 ccacacagag agttccagac aagttgagtt gttctgcgat tcgcactgcc atagctttag
    29401 ccttgtctac tagactttca gaaatgcggg ctggtacgat ggtcttagac aggatattgt
    29461 tgcggtggat attttcctga actgggaaaa acgtcacctc cttgccattt cctgacacga
    29521 tgacagaaat ctcaaggtca aagttgacaa attcttccaa gacgcagtct gctgagtctg
    29581 ctagcgcata ggctgcttcc aagtctgctt ctgaacgaat aaccttttgt ccatgaccat
    29641 cgtagccacc agtcgcagtc ttgaggacat agtttttcga caagtcgata tctgccaagt
    29701 ctaggctaga agtcacgacc ttgtagggtg ccacagtgac ttgagccttg tttgacaaaa
    29761 agtccttttc aaaaatacga ttttgcgaaa tgcggagcag atctgttcct tgagggagtt
    29821 gtccatcctt gataacggca tccaaaccgt cagcgtcgac attttcaaac tcataagtga
    29881 ggacatcgca acggtctgcc aactgacgga gggcatccac atcgttataa ggtgccacaa
    29941 tgatttccgc cacgcgagag gccgggcaat ccgccgcagg atccagcgcg ataaccttgt
    30001 ggcccatgta gatagcagaa atggccatca tctgacccag ttgaccgcca ccgataattc
    30061 cgattgtttt agatgagctc atttgacgac tcctctgcga tttttccttg ttcttcagca
    30121 aagttggcaa gtgcatccgc aatggactta tcttctacag agaggagacg gagggcaaag
    30181 agagctgcgt tagtcgctcc agcttcaccg atagccatgg tcgcaacagg caccccaccc
    30241 ggcatctgaa cgatagaata gagtgaatcc actccactaa gagcacgaga cttgactggc
    30301 acaccaatga ctggaagggt tgttttggca gctaccatgc ctggcaaatg cgctgcgcca
    30361 ccagcacctg cgatgatgat cttgatgcca cgactacggg cttcttctgc atgtttgaac
    30421 atgaggtctg gtgtacggtg tgcggaaaca actttctttt cgtaggctac accgaagcgg
    30481 tctaggactt ctgctgtttt ttgcatggtt gcccagtcgg attttgagcc catgatgatg
    30541 gaaattactg gtttcatttc ttctcctttt tcctcttttt caacaatcac cttagcttgt
    30601 gagggacggc agcagccacc aaaggtgtct catgcctaaa ttggaaaccc aaggtcttcc
    30661 aatttccctg aacgattgga ttatcatcca atcgttcttt cacaacggcg gtgattgttc
    30721 tatattagct cgctaacgct cgcaaaccat acgatcatta acgtattttg tgaattttta
    30781 tttttctttt tgataatggt cttaggtgat ggaggcggaa gcaaaccttc ggtttcattc
    30841 ctaaactttg agcctacggt ctccaagttt ccccctgacc agaacattca ctgttctggt
    30901 cttttcacca cgacgaccat tatcgtgttt ggcgactacg gcgcgttatt cttatatctt
    30961 tacttaattg ccttgcttcc gatatctgtt cggtagaaga gtccttctat tttttgttgg
    31021 tatagttctt ggtagatgct ggcttgggct tctttgacgg tatctgctgt ggtaacgagc
    31081 atataaactc gtccgccgtt tgagagcagt gctctgctat tttccgcaaa cttagcccct
    31141 gcatagtagg tgatgacatc gccttctgtc ttggctggca actcaacgcc cctttcatag
    31201 tctagcgggt agcccttgga tgcgacaacc acacccagag tcacaccctt gtccgtccac
    31261 atgatatttg gctccttgct atccaggata tctgtgatat tttgagcaaa gtcagaggtc
    31321 aagcgaggca agataatctg agtttctgga tctccgaacc gagcgttgaa ctcaatgact
    31381 ttcggtccat cagctgtcag gataagccct gcgtaaagaa ctcccagata agggcgacct
    31441 tctttaatca tcccttctag aactggcttg acaatggtgt caaccgctgt atcaactaca
    31501 ctctgtggta agtgtggaac tggcgcatag gcacccatac cacccgtgtt aggccctttg
    31561 tcgccatcgt aggcacgttt gtggtcctga gccgttggca tgatgtagaa cttatcacca
    31621 ttaacaaagg caaagagtga aaattcctct ccttcaagga attcctcaat aaccacgcgc
    31681 gcacctgagt caccaaattt attgtccaaa agcatctcat gagcggcttc gaccgcttgc
    31741 tcaaccgtct cagaaacgac gacacccttc ccaagcgcca agccatccgc cttgactacg
    31801 ataggcgcac catgcttttc gatataggcc tttgcttcct cgaaatctga aaatgtgcca
    31861 taggctgctg tcgaaacacc atatttgacc atgatttcct tggcaaaatc cttggaccac
    31921 tccagctccg ctgcagccct agtcggacca aaggccttaa gcccagcttg gttaaaatca
    31981 tccacgatac cagcagcaag ggcatcatct ggaccgataa aggtccaagc aacatcattg
    32041 gtctttgcga agtcaatcaa tttagaatgt tcggaaatag agatatttac caattccaaa
    32101 ccatccagag tcatcccatc attcccagga gctacaaaga ctttttcaac gtcttttgat
    32161 tcaagtaact ttttagcaat cgcatgctca cgaccacccg aaccgacaac aagcagtttc
    32221 attcctcaac ctcttttgcg aattatttac caatattata tcaaaaaagt tcgttttaat
    32281 cttgtttcag ttagcatttt aagccaaaaa aggaaagaaa ctgttttctt cccttttatc
    32341 ttcttaatgt ctaaaatgtc tcacacctgt aaagaccata gtcaagccgt atttatccgc
    32401 tgcttcgatg gattcttggt cacggacaga gccaccgggc tggatgatgg ccttaattcc
    32461 tgctttggcg atttcttcca cgttatccgc aaatgggaag aaggcatctg aagcaaggac
    32521 cgccccgttc agacgatctt tggcttggtc aatggcaagg cgaacagaag ccacacggtt
    32581 ggtttgacct ggaccaacac caagtgtcat gtggtcgttg gtcacgataa taccatttga
    32641 tttgacgtac ttgatagcct tccaagcgaa ctcaagagca gtcgcttctg tctcagttgg
    32701 ctgacgtttg gtcaccactt gccagtcagc tgggctttcc ttgaccacgt cttgattttg
    32761 cacgagaagt ccaccgacta cacctgtgta ttctgcttcc acttcgctag cctcttgagc
    32821 attaaatggc aaggcaagga tacgcaagtt tttcttttta ttgatcaaaa tagctagcgc
    32881 ttcatccgta tagcttggtg caatgatgat ttcgaggaaa acgccgtgca tcttctcagc
    32941 tgtcgcagca tccacctcac ggttgaggac gacaatccca ccaaagatag acactgggtc
    33001 agactcataa gcgtagtccc aagcagtctc aatgttatca gcctgaccga ttccacatgg
    33061 gttcatgtgt ttgagagcca caacagttgg acggtctttg aaatcacgaa taatacgaat
    33121 ggcagcatcc gcatcacgga tattgttgaa ggacaattct ttaccattga gctgtttggc
    33181 tgaagcaatg gagtagtctg ttggcaaggc tttttggtag aaatccgcgt cttgttgagg
    33241 gttctcaccg taacgcatgg cttgcttgag gtcataagtc aaggtcaact tttcaggctt
    33301 gctttcaccc acttgagctg tgaagtattc tgcaatcaag gcgtcataag ccgctgtgtg
    33361 acgaaatact ttggctgcta aacgttggcg agtttcataa gtcgtttcac cattggctga
    33421 caactcgtcc aaaaccacag cgtagtcagc aggatctacc acaactgtaa cactggcatg
    33481 atttttcgct gctgaacgaa gcatagatgg cccaccaata tcgatatttt caactgcatc
    33541 agcataagtc acatctggtt taaggatagt ttccttaaat gggtaaaggt tgaccaccac
    33601 aaggtcaatg agctcaatct tgttgtcctt agccgcttcc aagtggctat ccaagtcacg
    33661 acgagcgaga agccctccgt ggatatttgg gtggagtgtc ttcacacgac cgtccatcat
    33721 ttctgggaaa ccagtcatat catcgatagc aatggtatcc accccagcat tatcaagggc
    33781 aaccttagtt ccacctgttg agataatctc ccaaccaagt tttttgagtt cttgggcaaa
    33841 ttcaacaatg cccgctttgt ctgagacgct gattaagacg cgtttagtca ttatattctc
    33901 ctttttaatc attagttaaa caccaattaa atgcagtaac agtaaaacaa gataagcaaa
    33961 accaaggcta agcataccta agatattgat ccattttcta gtctgtgatt tgaacactct
    34021 tataaaaatt tgataaatca gtaagcctac acagacacct agcgtattta aagtcaaatc
    34081 cgttatatct gttataccta ttgctaaaat atactgtaag cactcaaaca ataaactaat
    34141 caagaaacct gtcccgacta ttcttaaact agataaatta gtttttatca aagggaaaca
    34201 aacacctaat ggaataaagg aaatcagatt aaataacatt tcagcaaaaa caatttttcc
    34261 atcgactatt agtggttctg aaaatggaat ccaattgata taccttggag ctaagaaaaa
    34321 tgctataaat tcaggatttg tttcaaactt aaataagatt ccccaagtta ataacatcag
    34381 atagacataa aatacatatc gagttgtttt tttagattct aacattacaa atacctccgt
    34441 ttgaagccgt cttcaacaaa cactgactcc aatttaaaat caaagatatc atatgaatca
    34501 tcaaaaagtc aatctgtaaa tagagccttc actacttccg gatacaacct gtactctgct
    34561 tcatggatgc gagcttcaaa tctgtcaatg gtatcatcag ctagtcgtgg cacacgaacc
    34621 tgtttgatga cctggcctgt atccacaccc gaatccaccc agtgaatggt cacaccagac
    34681 tgacccacgc cagcattcca agcatcctca atcccatgag ctcctggaaa ttctggcaag
    34741 taggctggat gaatgttgac aatccgacct tcataagccg acaataaggt tggtccaact
    34801 attttcatgt agcctgctag gcaaaccaag tcaatctggt gttcttccaa gagttcgaca
    34861 agggctgctt cgtagtctgc cttgctctca aactccttga gttcaaaagc ataggacaga
    34921 acgccgagct gctttgcacg ctcaagcaca taggcatcac gatggtctga aaagacaaac
    34981 tccactggaa attcttcggc aatcacctga aaatttgagc cattaccaga ggcaaaaacc
    35041 gctatttttt tcatttgata atgacacttt cgttttcttt cttgacgatg cgaccaattt
    35101 catagactgc ttcatccaac aattctttta cacgctctac attttcaggg ctgaccgcca
    35161 acatgagtcc cacacccata ttgaagattt caaacatttc ttcgtgttta atctgaccgt
    35221 atttttcaag ggctttgaaa attggaagca ctggaacttt gctttcatca atttcagcag
    35281 ctaggtcatc tgcaaacata cgagggacat tttcgataaa gccaccacct gtgatgtggg
    35341 caatgccgtt gaccaactct tctttgatga gcggcaagac agccttgaca tagatacgag
    35401 tcggctcaag tagaacttcc ttaagtttct tgccttccaa ttctggtagg acttcctcac
    35461 ctgtgtaatc cgcaaagaca cgacgaacca aagagtaacc atttgagtga atcccacttg
    35521 aaacaagtcc gagaagaaca tctccctcta ccacctttga accgtcaatg atttgagatt
    35581 tttcagccac accgaccgca aaaccagcca agtcatagtc gtcttcgccg tacatgcccg
    35641 gcatttcagc cgtttcccca ccgatgaggg cagcaccagc ctgcacacaa ccttctgcca
    35701 caccagcaac cacttgttct agcttagctg gttcattctt ccctgtcgct acgtagtcga
    35761 gaaaatagag gggttccgca cctgcagcaa tgatgtcgtt gacacacatg gccacacagt
    35821 cctgcccgat ggtatcgtgc ttgtcgtact tgatagccaa catgagcttg gttccgacac
    35881 cgtcagtccc tgaaatcaag acgggttctt taaccccagt cttggaaagg tcaaacatac
    35941 caccaaagcc accaagagct cccatgacac ctgcacgctc cgtacgggcc acgtgctttt
    36001 taatccgttc aacaacttca taacccgctt caacatccac acccgactgg gcatatgcat
    36061 ttttatttgt catttgtttt ttccttttct ttaatggaga atctgtcgcc tacttgtaaa
    36121 aactggtctt ttcttccaaa cttctacgat agtcttcttc gtagtcatag agaggcgttg
    36181 ggtagtcacc gtcaaagtaa gcgacacaga gaccaccgtt cggcgcatct gtttcgatac
    36241 caatcgactc aatcaagcca tcaattgaaa gataagtcat actgtccgca ccaatgattt
    36301 ggcgagtttc ttcgaccgta tgattggctg caatcagctc ctgacgggtc tggatatcaa
    36361 tcccgtagaa acatggatac gctagtgcag gacttccaat ggcaacgtga acctcagtcg
    36421 cacccgcttc tttcaagagc tgaacgatac gacgagaggt tgttccacgt acaatggaat
    36481 catccaccat gaccacacgt ttgcctttga caacacccga aacagcagac agtttcatcc
    36541 gcactccttg ctcccgcaat tcttgagtcg gttggataaa agttcgctgg gtgtattggt
    36601 ttttgatcag acccatttca tttggtaagc ctgattcttc cgcaaatccc atagccgcgc
    36661 ttagggaaga attgggcaca ccaactacaa tatctgcctc atgcttaaat tctcgcgcca
    36721 attgcgctcc cattctctta cgtgccgtat ggacattgac accgtggata ttagaatcag
    36781 ggcgagcaaa gtagatatac tccatagaac aaatcgccaa ctgggtatca tctgtatagc
    36841 tgtcatactg aatgccctcg tcatcaatga tcacaatctc acctggcttc aaatcacgaa
    36901 tccactcggc accaataacc tcaaaagcac aggtttcaga agatacaaca actgctccat
    36961 tagccatttt cccgatagaa agcggacgga aaccattggg gtcaagagcc gcaatcaact
    37021 tgtcctcaaa cagcaagata taggcaaaac cacctttgac aaggctgagc gcttccttga
    37081 ttttgcccat caggctagga ttatgactgc gacgaatgag atgagccaag atttccgaat
    37141 ccgaagtcgc actgaaaatt gctcctctat cttccaattc tttcttgaga gaggctgcat
    37201 tggtcagatt cccattatga gccaagccaa actgcatatc gtgaaaacgg aagaggaagg
    37261 gctggatgtt atctacagaa gcttcgccag cagtcgcata acgcacatgc ccaatcgcac
    37321 cagtccctgt caatttatcc aaattagcag gatttctgaa gacttctgat aaaagtccca
    37381 tgtcacgatg gcgctttagt tttccttggt cattggagag gattcctgcc ccctcttgac
    37441 cacggtgctg aagactgtgg agtccaaaat aggtcaactt agcagcatct ggatgtcccc
    37501 aaataccgaa aacaccacat tcttcattaa gagattttac ttcgtatgtc attttttata
    37561 cctaattttc atttgtaatc atcaagagct agatttacat gacttttaca ttactgatac
    37621 tatctattag aaaatctgta agtcttattt tccagtgaaa tgtttaaccg ctgacgcgaa
    37681 caggtgctgg tctttattgc ctgggatatt ttggaaaaga ccgtcctcat aacgttctga
    37741 gtggcccatc ttaccgatga tttgaccatt cttgctggta attccttcga tggcatggac
    37801 agaaccattc ggattgtact tagaatccat acttggttta ccgttaaagt caacgtattg
    37861 gctgaaaatt tgtccattgt cacggagctc tgcaaattcc tcagccgtca cgacaaactt
    37921 cccttcaccg tgcgaaacag gaatagcgtg gatatcgccc acttgcacac cagccaacca
    37981 tggtgagttg gtattggcaa tgcgagtttc caccatcttg gccacgtgtt ggttggcatc
    38041 attgtagaag agggttgggc tagtactgtt agcaacttca aagtttccgt agggtaggag
    38101 acccgatttg actaaggctt ggaatccatt acaaataccg ataatcaaac caccacgggc
    38161 gataaagcta tcaatagcca cacgcacttt ttcattaagc aggatattga cgataaactt
    38221 agctgaacca tctggttcat ccgcagccga gaatccacca gcaaagaaga gaatattagt
    38281 cttgtcgata ttgtcaacca tagtttcaac tgacttgaca atagcttctt cattcaaggt
    38341 cacgaatggc accaaattga cctctgcacc ttctttttcg aaggccttag ctgaatcata
    38401 ttctgagttg gttcctggaa agactgggat gtaaaccaca ggtttttcaa ccttttcttt
    38461 ggctttaatc acaacatctg atgccacagc tggtacttct tctagttctt tcgcttgggt
    38521 aaattctgtt gggtaaactt cttccaatgt cccttgaaat gcactgtcaa gcttgtgtcc
    38581 atctagcttc acaccgttga cagtcagtgt aaagtctgct ttcgtttgtc caaccttctc
    38641 tactccagca atttcttcag gagatgtgaa gacaaagccg cctaattgag ctgtcaaagc
    38701 tgtttcaagt tcaggcaagg tcacctctgc accaatatag tttccaaagg tagcaagagc
    38761 caaactttca actacaccac cgtatttgac agctgatgca gatgtcactt tatggtcagc
    38821 ttggatggct tcaaactgag caaaattttt cttaatcaag tcaaaatcaa tctctgcaga
    38881 gagggcttga cctgggatgt agtagatatt ttccccaaca gctttaaatt ctggagagag
    38941 caccttacgg ctatctgccg tcgtcacccc aaaggcaacc aaggttggcg gaacggtcaa
    39001 ttcttcaaag gtaccagaca tggagtcctt accaccgata gatggcaagc caagctgaat
    39061 ttgtgcttca atagaaccta gaagagcagc tactggctga ccgaaacgct ctgcttgttt
    39121 atccatacgc tcgaaatact cttggtaaga gaaacgagcc ttggaccagt tggcaccagc
    39181 agccaccaaa cgagcagttg cttcgataac cgcataagca gcaccgtggt atggagacca
    39241 ttcagctaca tatgggttga aaccttgagc aatgaccgac gcagtatgag tcacaccgtg
    39301 ttgaactggc aatttctgca cagatgcctc agttggtgtg agttggtaac gaccaccaag
    39361 tgggtgatta accgttgagc gtccaacaga gcagtcaaag atagtctgta atcctttttg
    39421 acttgcatgg ttgaggtcag atagaaccgt aagggtatct gattccagtg tttcagcaga
    39481 tgtttgacgc tcttctggga gtttgacatc cttgtccaca actttggcat cgacaaccac
    39541 gcgcacacca ttggtgtcaa ggaaacgacg ctccaagtca acgattgtct caccattcca
    39601 gtgcatgaca agatttggtt tttcagttac tatcgccacc acaacagcat caatattttc
    39661 tttgttacat tcggcaacga aggcatccac atcttcagga cgaaccacga ccgccatccg
    39721 ttcttgtgat tcagagatgg caatttctgt accattcaag ccctggtatt taagaggcac
    39781 cttgttgagg tcgatttcaa gaccgtctgc caattcaccg atagccacac agacgccgcc
    39841 tgccccaaag tcattggact tcttgatcag acgagtgaca ttgccattac ggaagaggcg
    39901 ctgaatcttg cgttcttcga tggcatttcc tttttgaacc tcagcaccag cagtctctac
    39961 agactcaact gtttgaacct tagaagagcc cgtcgcacca ccgacaccat cacgacctgt
    40021 tttgcctccg agaaggatga tcacatcacc tgcttcaggt ttttcacgga caacattgcc
    40081 cttgggagcc gcaccaacaa cggcaccaag ttccatacgt ttagctacaa agcctgggtg
    40141 gaagtattca cgaacgtagg ttgtggcaag cccaatctgg ttaccatatg aagaataacc
    40201 atgagctgct gttttagaaa tgacttgttg tggcaatttc ccagcacgag tttccgaaat
    40261 cggtgctgta atatcaccag cacctgaaat acgcatggct tggtaaacat aggaacggcc
    40321 tgacaacgga tcacgaatag ctccaccgat acaggtagcc gctccaccaa atggctcaat
    40381 ttctgttgga tggttgtggg tttcattttt aaacatgagg agccaaggtt ccttgacacc
    40441 atcaacatcc acttcaattt caactgagca ggcattgatt tcgtcagaga cttccatatc
    40501 atccaatcgt ccgttagcac gctcataacg accgaaaata gtcgccatat ccatcaaggt
    40561 ttgtggtttt tcagaccgac ctaactcctc gcgcatggca atatacttgt cataggttga
    40621 ctgcaattgc ttttgaaatt tagaagctga aaagtcgatg tgtttcaact ctgtctcaaa
    40681 agtcgtatga cggcagtggt cagaccagta agtgtccaaa accttgagtt cagtctcagt
    40741 tggcacgcgc ccgattgact taaagtagtc ttggataaag agcaaatcat ccacttccat
    40801 ggccatccct tgctcggcct tgtagcgagc aaagtcttct gctgtatagc tttcaaagaa
    40861 agtcaatttt ggaatggtct tgtctgactc tgaaaactcc tgcttggcaa tccctgtcgt
    40921 gatatccttg aaacgagaat caactggatt gagcaggtag tttttgacag cttccaactc
    40981 agtcgcatca atatctttat tcaccaagta aagttgggct gtgttgactg tcacgtcact
    41041 cgaacttccc aacaaaagca aggcttcctg tgacgaagct gcacgctggt caaactgccc
    41101 tggcagactt tcaatggcaa agaaagcata gttagcaaga tccgcctgca cagatacttc
    41161 atctaaaaca tggtcggttc cctgctcaga gaaaatgtgc ttctctgcag gtgcaaacaa
    41221 gtcctcagcc aagtcaaata catcatatac ttgcacaata cgaatacttt tcaagcttga
    41281 cagtcccacg ttgtgctgga gctctctaac caaactctct gacttgacct gaaaatcagc
    41341 ctttttttca acaaaaatac gtttatccat caattcttat tcctttattt cagctcttgc
    41401 aatattccca aacagccttg aggttgttat tttaaaccct gcaacttttc ccaaacaatc
    41461 tcataaacgt cggttagttc tcccaatcct ctacggaaaa catccttatc catgtggttg
    41521 ccatcagcgt cccacaagcg gcagttatct ggtgaaaatt cgtctgccaa gataatcttg
    41581 ccatccttgt caaaaccgaa ctctagctta aagtcaatca atttaagccc aatctcagca
    41641 aaccagactt tcaatagttc attgatacga cgcgtttctt ccttcaagta ggcaatctgc
    41701 tggtcacccg caatctgtag gaatttcaca tgctcatcat tgataaatgg atcatccaaa
    41761 tcatcatttt tgtagtaaaa ttcgacaatc ggagtctcca aggcgattcc ctcatccaca
    41821 ccaaaacgtt ttgaaaagga accagcagta tagttgcgga gcacgacttc caaaggaata
    41881 atcttaacct ttttattgag ttgttccgtg tctgaaagtt tctccacaaa gtgagtcgcc
    41941 acaccagccg catttaattt ctcaaaaata aaagatgaaa tctgattatt caagactccc
    42001 ttacctgcaa tctgctcctt cttgacaccg ttgaaagcag tcgcctggtc cttgtaagtt
    42061 gaaataataa gattttcatc ctcagttgta tagatatctt tagcttttcc cgaatagatc
    42121 aattgttttg acattttaaa gttcgccttt cgtattactt gagcacattt taccacaaaa
    42181 aaccaaaaaa cacaagaatt caaccgaata aatagcagat gtttttcaaa aattgtaaag
    42241 aaaaaactgc aagaagaatc tttctcacag tttaaaaatt atttaactct aaaaacacga
    42301 acattactct ttgttcaaaa attgatccaa ataataacgt aggtaacttt tcttgcccgt
    42361 aatcatctgt agacggcctt ccaccccgta cctaagtttt tcagcctgct ccgaagttag
    42421 attagtctcc gcctcgattt taaagaaatt ccctttctca gtcttagtag ctgtcgcatc
    42481 aatacttgta atagtagaat ctagaaaaag ttgattcccg gcatcatgag tcgtagtata
    42541 gcgaacagaa tcaccgacct tgattcttgc tacatctttt gaacttagat aggctgtgag
    42601 tttggctttc ccttctcttt ccaaagatgg ataaagttgg gctagtaggg caccttctgc
    42661 aaccatgcta gaatcactgg tctcaggatt aagatgaagc accccatcct cacttgccgt
    42721 aattttcccc ttgtctaaaa gatttccctg taccttctta cctgactctg cctccaagat
    42781 tttctgggct agaagggtca attcctgacc aacctttgcc aagtgttggg atttaaggga
    42841 ttccaattga ctgcttaacc ctgacgcata ggcttgctgg gtacctgaac ctgcatactg
    42901 gacacggtaa gtagcaagac tagattctaa ctgagaaatc tgtgcttcaa cctgtgcaac
    42961 tgcctgaacc ttagtttggg gattttcctc gccctgagac ttgtaggact ggtaaagaga
    43021 gtaggctaga ttctgactgg ccaaggaagc acctgtttcc atagctgact tagctgtctg
    43081 gtaatcgcga attttagcct ctgtttgatt gatgaggttg ccgatttcgg cttgggtttg
    43141 gctagctgct gcattctggg acgcgatggt ctcattttgt tgcgatgtac tagccctaag
    43201 actgcctgct tgactgatgt agtcgcgaaa ggtggcttgg tagccaaact tatcctcctc
    43261 tggaaagtgg ttctcccctt cttgcaggct cttttgcaga tactccaatt gctttttttg
    43321 atcctttagc atgtccaact gactggcata ggactccgct tggacaccct ctgccccttc
    43381 ttggtattga accaaaagat cccccttctt aaccagctta ttttcttcca aatgattgac
    43441 aagaatacga ttgttgctag ttgactggat atttgcaagg atacgactag gttcgacagt
    43501 agctctagtg gacaagctca tctccttttc tgcaacagtt gcaaagccaa gtaaaaacac
    43561 gagcagaagg gccatgggta caatcacact actggaaaaa ttatggtaac gacgattata
    43621 aaactccgca ctttctaaaa attctggttt catcctctcc tctttctagc tattgaccaa
    43681 atgggcgtaa aagccaccct gtgcaagcaa atcagcatgc tttccttctt cgacaatctt
    43741 gccctgatcc aagacaacca ccttctctgt ccgctcagca atagtcaagc ggtgagcaat
    43801 gaaaatcaag gtcttgtcca aagccatgag attatcgaca atccgcttct ctgtcaaaat
    43861 atccaaactg ctagtcgcct catccaaaat caagaccggc gcatctgtca agagagcacg
    43921 cgccaaagcg attctctgac gttgaccacc tgaaatccct gccccatccg aagtcaattc
    43981 tgtctgataa ttcagtggca tgcgctcgat atcctctcga atctctgcca attcgaccgc
    44041 ccgtaagata tcttcctgtg tcgtcccctc cttggctccc aaaagaagat tctccaaaat
    44101 cgttccgtta aagacatagg gctgttgagg cagatagttt atgtactggc gcagggcttt
    44161 tttatcaatc tgattgagat tgacactacc cagactaatc tccccttgac ttgggtcgta
    44221 aaaattaacc atcatcttgg ccaaagtcgt cttacctgac cctgaaatcc ccacaaaagc
    44281 caccttagac ccttggggaa cggttaaatt gatatccgac aagacgtctc gaccatagcc
    44341 atacttgtaa tgaacctgct tgaaggtcat atctcccttc atcaagctca aatcctcaac
    44401 tgttttcttc tcctcaaact cagaagctac tagatacact tcatttagac ggttattggc
    44461 aacctgcgct gtctgaagct tggtttgcag attgatgata ttttccaaag gattggtaaa
    44521 gtaaaccagc aaggtattat aggtaatcaa ctgccccaaa ctcatcttgc catccatgac
    44581 cagaacagcc cccatccaga gaatgccgac attaagcaag agatgggcaa cctttttcag
    44641 agccttttgc tgactctctg ctcgactata ggtaaaggat ttcttcagat aatccacaaa
    44701 ttccttgtca attttttggt aacgctgact ttcactggtc aaggacttga tagtctcaat
    44761 accgttgatg tcctcaatga tagaagaaga cagaaccgca ttggcttcca tggtatcccg
    44821 attcatcttt tcaaacggct tcataaaggc aaagataatc actgtgtaga taggaagcgc
    44881 caataaagtc atgaaaaaga gattggtatt ttgtgaaaat agaacaaggg aaataatgac
    44941 aaccgttgac acatctagga aaatcgaaag gatggtcgaa gccagcgcat cgatgatact
    45001 gttagcatct gtaaaacgag acacgatttc ccctgtcctg cgtgtcgcaa agaaggacat
    45061 agggaggtga aaaacatgct tgatatagga caaaatcacg tcaatcgaca agcgttgccc
    45121 caaaacaagc aagagatact cctgagcgta agacaagatt tgctggagga tgtagacgat
    45181 gactagccca atagaaataa tccctagtgt cgaacgcatc tgatctggca cataggtatc
    45241 aatgatagac tgcagataat aagaacccac aatgttaatc acggttacca agagtgttgc
    45301 caaaacgata ttggcaatca agccacgctg cttcactaat atagggataa aagagaacag
    45361 accatttttt tgttccttat gaggcttgta gtctggacta ggtgccataa aaagagtcac
    45421 tcctgtccat tcttccgcaa aacgatcacg tggcagtttg gtcaacttca ccccgggatc
    45481 tggatcggca atatgaatgc tatccttatc ctgcccagtc accacatagt agtggagcaa
    45541 tttcccttcc ttaagcacat gggcaacaaa aggaaaagtc aaatccggca agtcaaaaag
    45601 cgtcatatcc gccttaatgg ctcgcgtctc aaaaccaatc tcctctgcca ccttgaccaa
    45661 gcccaaagcc gtcgttccat ccatggtcgt cttagccaat tctcgcaagt gagccaaaaa
    45721 ataataacta ccatagtagc caaaaaccat ggctaatgaa gctacaccgc agtccatctg
    45781 atccacttgc ggacgatagt gacgtttccc aaatttcata ttcatctcct tttccctaat
    45841 actcaatgaa aatcaaagag caaactagga aactagccgc aggctgctca aaacactgtt
    45901 ttgaggttgt agataggact gacgaagtca gtcacatata tacggcaagg cgacgttgac
    45961 gtggtttgaa tttgattttc gaagaatata aactcatctg ataacaacta tcttacccca
    46021 atcactaaac aaaaactgga cttccttccc aaatgagcct atctccctcc caactgcact
    46081 ttttggataa aaaaataggc agaacaagag tcctgcccac taaaaatatg ctattgaaag
    46141 gaaaatgatt ttttactgac accctaggca agcttgaaac aaataaatca ttgatagtaa
    46201 cacttacgac atcctattta ttacttcaat ttctaatgtc cccgctattt ctaagccacc
    46261 tctggtatga tctattccta gtcagaatag ataacaccgt tctaaattac gattcatagt
    46321 tactcctttt ttgactcgtt actataagac tcctatttac atgcgcaaaa taattttgat
    46381 tcagattcat catttatagt tttgattatt ttttaacaga tttttctgtt ttcttctccg
    46441 aagactttaa aaattttttt ctaattaaat catccatata aggaccaaat ataccaacta
    46501 ctaaaccaat aataaaactt ttaaaatcca taattaccac caacatgtcg ctgcataggc
    46561 cacacctcca agtatagctc ctcccacagc accagttgct gcaccttgcc atgttcttgt
    46621 tttaattcct agctgaagac ctcgtactgc tcctcctcca acacctgctt tggcaaaatc
    46681 tccccaattg catccgccac cttcaacgca agcaagcatc tcagtatcca taatttcaaa
    46741 ttgtgacatc ttttttgtat tcataacgaa tacctctttt ttatttttaa tatttgtctt
    46801 gttacaaact tgacaagttt agtataacag tatctattaa tttttttcat ccaaatcttg
    46861 aattggcatc gaaacgtctt gaattagctt ttttgtttca aaatcatctc tatttttaaa
    46921 aaagatgttt tctaatcact tttttactat tagacttcct gcaaaactag aatcctagtt
    46981 catgattgat aataccagca atcaaattca ttcgtaatcc gaagcgttta cgatgatttc
    47041 gataggttgt tgaaaacatt ttaaacgttt ttactttggc aaagatattc tcaaccttgc
    47101 ttctctcctt agatagcgca tgattacagg ctttatcttc aactgttagc ggcttgagtt
    47161 tgctggattt acgtggagtt tgtgcttgag gatatatctt catgagccct tgataaccac
    47221 tgtcagccaa gattttacca gcttgtccga tatttctcat ttctaaaaac catctacttc
    47281 cgttgacttg gaactccgat tgctattttc cttgaatgat tttaaccaca tctcctacgt
    47341 tttggagttg gtcaatttct tcatcgctga tttcgatact aaattcatct tccagagtca
    47401 agataaactc catcaagtca acagaatccg catccaaatc gtctttcaga ctcaaggatt
    47461 ctgtcacgac aaagtcctct ccctgtcgct cttggataat ggtcacaata cggtcaaaaa
    47521 tttctttttc tgtcatctct tttattctcc tgaaaattca cgcgcagtct gggcaaccac
    47581 gtctgtttct agcatggtac ggatctgacg aatcgtacta taaacagcct tggcatcgct
    47641 tgagccatga gtcttgacaa caggtgcctt aacaccaaac aagaccgctc caccaacatc
    47701 tgaataattg agctgttttt tcaaacctct gaggctgtcc ttgagaagga gggcacctag
    47761 tttcgctcga agaccaccac ctgtaatagc tgtcttgagc aagcccatga ttcccatagc
    47821 tgtcccttcg atggatttga gcacagcgtt tcccgtgaaa ccatctgcca caacaacatc
    47881 tgcaacgcca ttcatcaaat cacgcgcttc cacgtttccg ataaagttca aactttcatc
    47941 agccgccagt aattcataag tttccttacg aagcgggtcg cccttgctac tctctgttcc
    48001 gttgttgagc aaaccaacgc gtggttgcgc aatgccacgg acatttttag catagaagga
    48061 acctagaacc gcatattgat ggaggtgctg ggctgtattt tctgcattag caccgaggtc
    48121 aagcatgtca aaacctttcc catctacagt cggcaatgtt gacataagtc caggacggtc
    48181 gatattcttg atacgaccca cgatgaagaa tccagcagcc aacaaagcac ctgtattccc
    48241 agccgaaagg acagcgtctg cttcaccatc tttgacagcc ttggctgcca ataccatact
    48301 ggcatttttc ttattccgaa tagctctcgt aggttcatca tccgaatcaa tcttctcatc
    48361 cgtatggata atgctgacgc gctctgtcgc tgtcagatat tgcttgattt tagcttcatc
    48421 tccgtagagt tgaacctcga tatctgaaaa gtcagatagg gcttgattga caccctcaac
    48481 gatggcctga ggtgcgtaat cgccccccat ggcatctact gcgatttttt tcatttcttt
    48541 tcctctttta gtaattgtcc ccagtctgct agggaatcaa taaatttctt tgattttagg
    48601 tgaatcccaa cgtactcttc atatagttga tccataaatt ggcgtagctc ttgcttgatt
    48661 ccaggcttga gcgaaatggt ctccaaagtc tcaaaatcaa tagcttgaaa ttgattgagc
    48721 agatagggga tattgggatt gagatggcaa cgtctctcat cctcatgata atgctctgga
    48781 cagagacagg ctccatattt gaaagaaaag tcaaaagcct gaccaacccg atggcagaag
    48841 acacactcat taaaattgag gctgattcca aatcgagtca aaatttgaat ttcaaaaata
    48901 ttggtcaaaa cctgataatc caagcctgct tccatcaact ccaaagtctt ttgcaaaaaa
    48961 gcaaacaagg gagcatcctg ctgattgtcc tgcaaactag catctgcaag agctgccaca
    49021 taggtcgcat aggccatgac aaagaggtca ctattaatct tgggaaaagt catgacctca
    49081 tgatagtctt cgatgtaact gagtccgtca tcattgattc gcaagagaaa tcgtgccagc
    49141 accaagggct gaataacagg cgccagctta gactgaccag cgtgtttgac aaaaaacatg
    49201 catttgccaa cctgctctgt aaaaattttg acaagcttgt catcctcacg aaaattgcga
    49261 ttgtaaagca ccaagccttg actcgtgata gactgaatca tgcttctctc atatactcct
    49321 caagtcgttt catggcttct ttgatagtct ccatgctggc tgcataagat aggcggacgt
    49381 agccttcccc gtaacgtcca aaggctgcac cagggataaa ggcaacggcc ttcttctgag
    49441 caaaatcctt cagaaaagca aaggagtctt gattgtagcc cgctggaatt ttagcaaaaa
    49501 tatagaaggc accgtctggt ttgataatct caaaaccaag agcagtcatt ttttcgatga
    49561 tatagtcccg acgctggata tattccttct tcatgggctc cgcatcattt ttaccagccg
    49621 tcaaggcttc taccgcagca tgttgcgcca tggtatttgc ggcagtgacc aagtattggt
    49681 gactcttgat taactgggat gtgaaggccg caggagcgaa aatcagcccc aaacgccaac
    49741 ctgtcatggc atgcgattta gacaaaccat tgataataat agcctggtct ctcaacatcg
    49801 ttcctagaga cacatgggct tcgcctgtgt aggtcaattc tgagtaaacc tcatcacaga
    49861 caacaaaaat ttcgtacttg cgtaaaacaa ctgccaaggc ctctaactgc tctcgactgt
    49921 aggtaattcc tgtcggattg gctggatagt tgagaataac cgccttgagc ttatcaccct
    49981 gctccaaaat ggccttctcc aacatctcag gagtcaagac aaaaccattt tcagtcgtat
    50041 caatctcaac aatttctgcc ccaactaagt taacaatcgg ttcatagcct ggataagcag
    50101 gagctggcaa aagtaccttg tctccctctt ccaaaatagc cgtcaaagtc gcagataaag
    50161 cctctgtcgc cccaattgta accaagattt cattttcagg agcatagtcc agttggtact
    50221 tttccttaac aaagtcacta gctgcctgac gtagagtcag cagaccactc atccctgtat
    50281 agtaggattg gttctgatca atcgctcgct tggccgcctc cttgacatgg tctggcgttg
    50341 taaaatcagg ttcccccaag gtcaaacgca agaccccagg aatctccgaa atagcctggt
    50401 caaactgacg aatcaacgaa acttgaattt tatctaactg tttattaaag cgcttagtta
    50461 agtccataga tacctcctac tttcaaaacg tatctctatt atactacaaa agccccccga
    50521 ccgcaaaaat acattagagg atagctttcc actttctatt ttacttttcc tgtttatagg
    50581 tctataatgg taatagattt tatttggagg tttttatgtc atttctatca aaaaatggag
    50641 caggtatctt ggcctgcctt ctcatttcta tcgtatcttg gtacttagga ggattcttcc
    50701 ctgtgattgg cgcgcccgtt tttgccattt tcataggcat gctcctacat ccctttctct
    50761 cgtcctataa acaactggat gctggtttga cctttagttc caagaagttg ctccaatatg
    50821 ccgttgtctt gcttggtttt ggtctcaata tctcgcaggt cttcgcagtt ggccaatctt
    50881 cactccctgt catcctgtcc actatctcaa tagctctgat tattgcctac ctcttccagc
    50941 gtttctttgc cctggataca aaactggcta ccttggttgg agtaggttct tctatctgtg
    51001 ggggttctgc cattgcagcg acagcgcccg ttattgatgc taaggaaaag gaagtagccc
    51061 aagccatttc cgttatcttt ttcttcaatg tcttggctgc gctcatcttt ccaaccctcg
    51121 gcacctggct tcatctatcc aatgaaggct tcgccctctt tgcagggact gcggtcaacg
    51181 acacttcctc tgtaacggct gccgccagcg cttgggacag tctttaccaa agcaataccc
    51241 tcgagtctgc aaccattgtt aaactcacac gtactttggc cattatccct atcacgctct
    51301 ttctatccta ctggcaaagt cgccaacaag aaaacaagca aagcctgcaa ctgaaaaaag
    51361 tcttcccact ttttatcctt tactttatcc ttgcctctct cctcactaca ctactcacct
    51421 ctctaggtgt gtccagtagt ttctttactc ctctcaaaga actctctaaa ttccttattg
    51481 tcatggccat gagtgctatc ggtctcaaaa ccaatctggt cgctatggtc aaatccagtg
    51541 gaaaatccat tcttcttgga gccatctgct ggattgccat catcctcacc actcttggta
    51601 tgcagaccct tatcggcatt ttctaacact cttcgaaaat ctcttcaaac cacgtcaacg
    51661 tcgccttgcc gtatatatgt tactgacttc gtcagttcta tctgcaacct caaaacggtg
    51721 ttttgagcag cccgcggcta gtttcctagt ttgctctttg attttcattg agtataacac
    51781 aaaaggtagc ccatcagcta cctttttctt atgcttcctc aatcaagcga gtatgttctc
    51841 tcttgataca gcgattcatc acgatatcat cacatccacc atcacgcaaa atctctttcg
    51901 cttctaaact ttcaagtcct agctgtgccc aaaaaatctt ggcatcagct ttgagaaaat
    51961 cacgcgccac atcgggcaga aattcactgc gacgataaac attgacaata tctacaggaa
    52021 aaggaatttc agcgaggcta gcataagcct tttcacccaa gatttcgcca cctgccgcct
    52081 tgggattgac tgggatgatt ttatagcccc gagcctgcat ttcctttgtt actcgattgc
    52141 tggttgtttc ttcacggtca gacaaaccca ccacagcaag ggttttactc gttgcgagat
    52201 actgacgaat cacgccatca cttggattga taaattcttg actcatagaa atcctccttt
    52261 ttcatcagta tagcacattt tgaaaaggtt tgcagaatta tactacaaaa aaggaggact
    52321 agcccccttt ttatttagcc tcgtaccagg ttgccccttc attctcatct gcgataagag
    52381 gaacactgag ttgaatggct tcttccatgg tttgtttcac caattttttc atctctacca
    52441 attcagattt aggcacttca aggacgattt catcgtgcac ttgtaacagc atcttagtct
    52501 gataaccacc tgcaaccaag gctttatcca gctgaatcat ggcaatcttg agaatatctg
    52561 ctgccgaacc ctggataggt gagttgatag cagttcgttc cgcaaaacca cgaatattga
    52621 agttgcgcga attgatatct ggcaactcac ggcgacgctt aaagagggtc tctacatagc
    52681 ccttatcacg cgcctcccgc accacttcat ccatgtagtt tttaatacct ggaaaacgtt
    52741 caaagtaggt atcaatgtag gctttggctt ccttacgact aattcccaaa ttattagaca
    52801 agccaaagtc tgaaatccca taaaccactc caaagttaac tgccttggca ttgcgacggt
    52861 cgtttgcagt cacatcatca ggacgctcaa tgccaaagac ccgcatggct gtcgaagtat
    52921 ggatatctgc cccctcttgg aaggccttaa tcaagtgctc atccttagaa atatgcgcca
    52981 aaacgcgcaa ttcaatctgt gaatagtcag agctgagtag cacactatcc tcccactctg
    53041 gcacaaaagc cttccgaatc aagcgcccct gttccaatcg ggcaggaata ttttgcaagt
    53101 ttggatccac actagacaaa cgcccggtct gggtcaaatc ctgcacatag cgagtatgaa
    53161 tctttccatc agccaaaatc cagtcctgca agccaattac ataagtagat tgaatcttag
    53221 caatttgacg gtaatccagg attttcttaa caatcggagc aataggagcg agacgctcca
    53281 aaacatccac tgctgtcgaa taacctgtct tggttttctt agtgtattct agaggaagtc
    53341 ccaatttctc aaagagaagc acgcccaact gcttaggcga gttgacatta aactcctcac
    53401 cagccagctc gtaaatctct tgagtcagtt tttcaatgac aagctcattt tcagcctgca
    53461 tctcaagcaa ggtctctttc ttgaccataa tcccagcaat ttccatcttg gcaaggacaa
    53521 aagccagagg ttgctccata tcataaagaa gctctaattg cccattttcg ctgagttttt
    53581 caagtaaaat aggctctgtt tctaccaaaa cagcaagttt acaagctaag tgttccaaga
    53641 atttctcacg ttcaggaatg gcctttttaa cacccttacc gtagaaagtt tcatcatcaa
    53701 ccaagtaagt ctgaccataa agactagcga tggtcgcaat ttcattgtcc tccacagtcg
    53761 aaaggaggta tttagccaaa cggatgtcaa aagcaggcgc ctgcaaatcc acaccaaaac
    53821 gttgcaaaag aactttaacc ttcttaaagt cataaactct cagagatgtt ttttctaaga
    53881 aatccttgaa aatcgggtct tgcaacagct caagcttgtc tgtggcatag agcttatccc
    53941 cacaagacca ggcaaatcca accaaattat ccgtatggta attctcacca aaaagctcaa
    54001 agtggaagat agactcttca ctcagcatat cttgactgat ttggtcaaca atagtaaaat
    54061 ccaaactctc agacacatca gctgacgaca catttaaagc ctgcttgagc tgtttgaatc
    54121 ccatctcatc gtaaaatttc ccaagatttt caacatctgg accactatag accaagtcct
    54181 ctaaaccaat cgcaatcggt gccttggtat caatggtcgc tagtgtttta gacaaaaagg
    54241 cctgttcctt gtcattgatg agattttcct tcatcttaga agtcttcatt ccatcaatat
    54301 tttcataaat cccctcaagc gaaccatact ccagcaagag cttaataccc gtcttttcac
    54361 cgactttggt caccccaggg atattatccg acttatcacc catgagcgcc ttgagatcga
    54421 taaactgagc tggtgtgagg cccatttctt ccatgaggta atctggcgta aaggcctcaa
    54481 actcagccac acctttcttg gaaatttcaa ccaccgtatg ctcatccgtc agctgaatca
    54541 aatccttgtc cccactgaca atggtaatat caaaaccatc ctgctctgct agcttatcca
    54601 gcgtcccaat gatgtcatcc gcctcatact gagccagctc atagtgacga atccccatat
    54661 gatccagcaa ctcacgaatg aaaggaaatt gctcacgaaa ctcatcagga gtcttggccc
    54721 gaccaccctt atagtccgca tacatctctg tccggaaggt cgtctttccc gcatcaaaag
    54781 ccaccaaaat atgactcggc tcaacccgct ccaacaaatg actcaacatc aactgaaaac
    54841 cataaatcgc attggtatgc aaaccagccg cattcttaaa acggtccaac tgctgataca
    54901 gcgcaaaaaa cgcccgaaaa gctacagaag acccatcaat caataataat tttttcttat
    54961 ccatacaccc attataaagg aaagaatcaa aaaataccat tgggaagagc tagagcaagt
    55021 atttttcaaa ctttttccga ataaatagat agagccagag aatttagtaa acctagattt
    55081 aaaaatgtgc tataatatag tatattgaat ctataatagt acaccttgac tgctaaaata
    55141 tttctataaa ttaatttgac tttcctgata gagttattca catcttattt caactcacta
    55201 tagaaggagg aataggagga ttctcagaca tccgggcatc agcccaacta atgatttgat
    55261 tgctaagaaa atattcagca atccagaaat cacttgtcaa tttattcgcg atatgctgga
    55321 cttgccagca aaaaatgtga ccattttgga gggaagcgat attcacgtat tactctccat
    55381 gccttactcg gtgcaggatt tttataccag tatagacgtc ttggcggagt tggataacgg
    55441 tactcaagta attattgaga ttcaagtcca tcatcagaat tttttcatca atcacttgtg
    55501 ggcttacctg tgcagtcagg ttaatcaaaa tcttgaaaaa attcgtcagc gagaaggtga
    55561 tactcactag atctacaaac acatcgctcc tgtttacgtc attgctatcg tggatagtaa
    55621 ttatttctca gatgacctgg cttttcatag ctttagtatg cgcgaagaca caacaggtga
    55681 ggtattggcg attaccaaca atggacagga aaaccatctg gttaagatgg cattcttgga
    55741 attaaaaaat acagagaaac cagcaaagac aaggttcgca agccatggtt ggagtttttc
    55801 gacaacaagc cctttaccca gcaaccgcaa cgagccatta cccaagcaaa tcaactgctg
    55861 gactacaaga gctggtccga ggaggacagg aaaatgttta gtcaactaca tatgcgagaa
    55921 gaacaagtct tgttagcaca ggactatgcc ttggaaactg ctagggctga aggccttgaa
    55981 caaggactag agcgtgggaa agttgaagga agggcagaaa ggaaactttt tgccttccta
    56041 gacatagtac gccaaggtct tctgacttct gaggttgcca gccagcaatt aggtatgtca
    56101 gtatctgaat ttgaggcact gttgtaaaat ggctccataa tatccatagg ggatttaccc
    56161 actatggata ttatggagcc tattttgtgt aaaaaaaagt cccatatgac ctataatgaa
    56221 aagcgacaaa acaactcatt agaaagaatc atatggaaca attacatttt atcacaaaat
    56281 tactagacat taaagaccct aatgtccaga ttttaaacat catcaataag gatacacaca
    56341 aggaaatcat cgccaaactg gactacgacg ccccatcttg ccctgagtgc ggaaaccaat
    56401 tgaagaaata tgactttcaa aaaccttcta aaattcctta tcttgaaacg actggtatac
    56461 ctactagaat tctccttaga aagcgtcgat tcaagtgcta tcactgttca aaaatgatgg
    56521 tcgctgaaac ttctgatgac gtacagtcat atttcttctc tttttattat atcacagttt
    56581 taaatctagc tttactagat tcaccgctac tatctattta ttcggaaaaa agacgaaaaa
    56641 acctgagaat catctcaggc ttggtcatta aatttttttc tcaatatcga aaagtggaga
    56701 aagtggtcgt ttttcatgaa tacgtacgat agcatcccct aggagatgag cgattgaaat
    56761 ctgctcaatc ttatcaatca aacgctcttc tggcagatag atggtatcca aaacaaccaa
    56821 tttcttaata gctgattttt ggatattgtc cgtagcagga ccagaaagaa ctgggtgcgt
    56881 acagcttgca tagacttcaa cagcaccagc ttccgcaaga gcatctgccg catgacaaat
    56941 cgttccagcg gtatcaatca tatcatcaat caagatacaa gtcttgcctt caaccttacc
    57001 gatgatgttc ataacttcac tagtattcat cttatcaacg ctacgacgtt tatcaataat
    57061 agcgatagat gttttcaaaa attctgccaa cttacgagca cgagtcaccc ctccatggtc
    57121 cgggctgaca accacatagt cagaaccaac cataccacga cgctcaaaat aatctgcaat
    57181 cagaggagca cccatcaaat gatccacagg aatatcaaag aatccttgaa tttgcgcagc
    57241 atgcaagtcg atggtcaata aacgatccac tccagctact tcaagcatat ttgcgacaag
    57301 ttttgaagtg attggctcac gcgctctcgc ctttctatcc tgacgtgcat acccatagta
    57361 aggcatgaca acattgacag attctgcact cgcacgcttc aaagcatcta ccataatcaa
    57421 aatttcaagc agattgtcat ttacaggcga actagttgat tgtaggataa agacgtgttt
    57481 cccacggatt gattcttcaa tgttgacctg aatctctcca tctgaaaatt ggcgaacact
    57541 tgatttcccc aactctatcc caatctcctg cgccacacgt tctgccaatt ctttattaga
    57601 agaaagggca aacagcttta aatcagaaaa agacatgatt tcctccggta tatatgtatc
    57661 gcttgtgctt ttcacaagat tttccatcta ccattgtagc gctttttgca ctatttttca
    57721 atcaaaaata aaagaagggc accatatttg tacccttgca tcattctttt gaaaaatatt
    57781 ctaggtcatc aactcattgt gtttctcaac aaagcaataa gcatgataat aaccatagag
    57841 agcaatagcc gtaaccactg gaatcgctaa aggcaactct gtttccaact ccacaaaagg
    57901 agagttaaac aataagaccc tgtttcttgc caaccttctg tcctttatag gctctgtaaa
    57961 gcaagtaaac acctactaca gctagacctg aaaaagtcca gtgagaggca attccaagta
    58021 aaaacaaaaa ttttaatttt cgcacaggaa tcaaagccaa aacaaaaaca agtggcaata
    58081 atttcaaggg ttcttctacc aaaggagcca caatagcact ttcaaaggca tttaaaaatg
    58141 gactatctgg gaaaagaacc cccagtaaat catgtatata agtattagca aaactagaca
    58201 accagcctga aaggaacatc cctcccaata aagacagaat caaaaccttc tttggcaatt
    58261 cccatttttt cccaatatgg aagagaaaat aaagagccgg aatcatgtaa aagagagcta
    58321 gaaagataga aactcccatt agtccatatt ccgcacctga cctcgaaccg tccgtatagt
    58381 agatggtttc atactgtaaa ccaatacata gcaataaaat actgcttttc ttcatacact
    58441 ttctttctaa atgaagtatt tataattcta cgactgtcat acttcctgta tcaacattgt
    58501 aaatagcacc agagataatg acatcgtctg gtattagggg agactcgata agcagttgca
    58561 tatcctcgcg tacactctct tctatatctt ggaagggcaa gaaatcctgg tctgacacat
    58621 ctacacctaa ttcctctttt aaatactcct gaaaaggttc attttcaaag gtctgagcac
    58681 cacagtctgt atggtgcaat accacaatct ctcttgtccc catttgttgc tgggaaataa
    58741 ctagcgaacg aatcatgtct tcagtcactc gaccacctgc attccgcaag atatgagcat
    58801 ccccaagtgc caagcccaga gcttgcgcaa cgtgcagacg agagtccata caggtcacaa
    58861 tagctactct tgttttgggt ttaagtggca gatttaactg cccatgtagg gcaacataag
    58921 cctgattagc ttgcataaac tgttcaaaat acgacacgat tccctccttg aaaatttgat
    58981 agtcaaatat ttctcctatc ttatcatttt taagagaatt tgtcacggat tatgcaaaga
    59041 cctttttcaa aacttcctga atcgttgtca cgccaatgac ctgaatttcc ttaggcagag
    59101 tgattcctgt caaggaattc ttaggtacat aaatcttagt aaagcccagt ttagcagctt
    59161 cgttgatgcg ttgctcaata cgattcacgc gccgaatctc tcctgtcaag cccagttctc
    59221 cgacaaaaca ttcctgagga ttagttggct tgtctttgta gctcgaagca atagcaactg
    59281 caacagccaa gtcaatggca ggttcatcca atttaacacc accagcagat ttgagatagg
    59341 catcctgatt ttgcaagaga agccctgccc gtttttccaa aacagccata atcaagctag
    59401 cacggttaaa atcaagtcct gtcgtagtac gcttggcatt tccaaacatg gtcggtgtta
    59461 ccaaagcctg aacctccgcc aaaatcggac gcgtcccttc catggttaca acgatggagg
    59521 aaccagtcgc cccatccaaa cgctcttcta ggaaaacttg actcggattg agtacctcaa
    59581 ccaagccgcc cgactgcatc tcaaaaatcc caatctcatt agtggaacca aaacgatttt
    59641 tgaccgctct caaaatacga aaggtgtggt gacgctcccc ttcaaagtaa agcaccgtat
    59701 ccaccatatg ctccaacata cgaggcccag ccaaggttcc ttctttggtc acatgaccta
    59761 cgataaagat ggcaatgtta ttggtcttgg ccaactgcat gagttcagcg gtcacttcac
    59821 gcacctgaga aacagacccc tgcacccctg aaatctcagg agacatgatg gtctggatgg
    59881 aatcaataat gagaaagtct ggctgtatac gctccacttc tgcacgaaca ctctgcatat
    59941 tggtctctgc atagagataa aactcactat caatatcacc taagcgctct gcacgtagtt
    60001 taatctgctg ggcagactcc tccccactga catagagaac tgtccccact tgggacaact
    60061 gggttgagac ttgtaggaga agagttgatt tcccaatccc aggatcccca ccgataagga
    60121 cgagacttcc tggtaccact ccgcctccaa gcacacggtt gaattcctcc atctccgtct
    60181 tggttcgatt gacattgatg gaagtcacct cagctagttt catgggcttg gttttctcac
    60241 ctgtcaagga cacacgcgca ttcttaactt cggcaacctc aacctcttcc acaaaagaag
    60301 accaagaccc acagttgggg caacgtccca gatatttagg ggaattatac ccacaatttt
    60361 gacatacaaa tgtcgctttt ttctttgcga tgacaaacct ctttctatat ctctaactca
    60421 cactcaatca cttggcaaaa atcaatcttc tcatttggca caaactggcg catgagcatt
    60481 cgatgagcaa caactaccac agtctgatgt tctcgatact tagacataca ttctagaaac
    60541 cgagacttca tttccgtagc tgtctcatat tgaataggac tattaggaag caactccccc
    60601 ttgttttcta aaaacagtct tctagctgtt tcaaagtttt ctattcctgt tttatagacc
    60661 tgccattcat gtaataaagg ctctactctt aaaggaagac ccgtagcaca gaccacatac
    60721 gaagccgttt ctaaagctct tgtgactgca gaagatacga ttatttcagc tgacgagagt
    60781 aaaggatttt tgctcaattt ctggacttgc tgccgtccca tctcagacaa gggtgccaaa
    60841 tctatcccaa atcctatata agaacgctcc tctaactcac ggtaatctgg ctccccatga
    60901 cgtacaaaga taatcttcat tctagtgccc tgtcgatcca aatccaccag ttcgaacgcc
    60961 atcagctgca tctccatctg caattaagaa agtagcaaaa acagcctgga caatacgttc
    61021 cccaacttca agaacaacct cttggtctgt gatattcttc atctgcacaa aaatatgccc
    61081 ttcatttcca ggatttccat aataatcccc atcaatgacc ccaactgagt taattaaaac
    61141 caagcccttc ttacgaggat ttgaagaacg atcatagagg tagagaacct cagtcggctg
    61201 catataagcc ttaacccctg ttggaaccaa gacaatctct cctggcgcaa caactgtacg
    61261 cacagcaacc tttaagtcgt aaccagccgc atgcgctgtc tcacgcttgg gcaataaatt
    61321 ttcatctgta aaactcgaaa ccaattcaaa accacgaatt ttcataattt tctcttttct
    61381 attatcattt attctagatt attctatctt atttattcgg aaaaagcacg aaaaaagagc
    61441 acacaattca aatcgcttag ggctgctgga ttcctcccct gacccgcttc acgcagaact
    61501 gttgctccac tatttattat atcacattcc tattcatttt aaaagcaaaa ttattttttc
    61561 cgtctatttc taaaaaaatc ctgcatgata gctgcgcatt catcttccaa aattcctatt
    61621 tcaacctcca cacgatgatt gagacgctca tctgtcaaga tattgtacaa actcccagca
    61681 gcgccaaatt tctggttttt agccccatag accacatttg gaatgcgggc aagcccaatc
    61741 gctccactac acatgacaca aggttcaatg gtcacaaaaa gtgtgcaatc cagcaagcgc
    61801 cagctctcct cactcaagtt cgcatcctct atagccataa tttccgcatg cataaccgct
    61861 cgctgtaatt cctcacgcgc attatgccca cgaccaatga tttccccatc tttgacaatc
    61921 acacaaccaa ttggaatttc atcgtgttca agagcaatct cagcctctct caaagcctcc
    61981 ctcataaaga cttctttttc ttcaagcgta taatacatct cttttctctt ttcctactta
    62041 tcgattttat tattatatca tgaatcccaa cacaaaagaa gccaccgaat gcggtgactc
    62101 tatagggaga ttattatgaa aaagaaaagt ttaggatatt tgttacaaca agttaggagg
    62161 tcttcttgta accgtctata gtatacccga cctatcttaa acaaatctta aaaatctctt
    62221 aggaccaaac actttctaaa atatttgttt gttcacgacc aggacctact gagaaagtag
    62281 aaatacgaac gccaaccaat tcactcacac gacgaacata gttacgcgca ttctcaggaa
    62341 gatcttccaa attgcgaact ccggtaatat cttctgacca acctggcaac tcttcataga
    62401 taggcttgca acgtttcaat tgctcaagac tagctggata gtagtcaata cgttgaccgt
    62461 caagatcata ggccacacag attttcacag tatccaaacc gctcaaaaca tcaatagagt
    62521 tcaaagaaag gttagtaata ccagaaacac gacggctatg acgcatcaca actgagtcaa
    62581 accaacctac acgacgtgga cgaccagttg ttgtaccata ttcatgaccc acttcacgga
    62641 tacgttctcc cacttcatca aacaactcag ttgggaaagg accatctcct acacgactcg
    62701 tataagcttt acatacacct acaaccttgt caatcttgct tggaccgaca ccagaaccaa
    62761 ttgtcacacc accagctaca gggtttgatg acgtaacaaa tggataagta ccttggtcga
    62821 tatctagcat aacaccttgt gcaccttcaa aaagcacacg tttgccatta tcaagcgcat
    62881 cattcaagat aacagatgta tctatcacgt atttcttgat ttgttgacca tattcgtaat
    62941 attcttcaaa aatatcatcg aaaacaatcg ctttactgtc atacaatttt tcaaaaagac
    63001 gattcttttc agcaaggtta cgttctaaac gctcacggaa aatatcttta tctaaaagat
    63061 ctgcaatacg aattccaaca cgagcagcct tgtccatata agctggacca attcccttaa
    63121 ttgtcgtacc aatcttattg tcgcccttag cttcttcttg caagcgatcc aactcgatat
    63181 gataaggcaa aataacatgc gcacgatcag aaatacgcaa gttatcagtt gttacacctt
    63241 cctcatgaag atagctcaac tcttttacaa gagatttagg atttacaacc ataccattcc
    63301 caatgacaga tattttttca gggaagaaaa tcccagatgg aatcaagtgc aacttaaatt
    63361 tcttaccgtc aatcacaatc gtgtgaccag cattatcacc accttggtaa cgtgcaatta
    63421 cttctgcatt cgctgaaagg aagtctgtaa tcttcccttt accttcatca ccccattggg
    63481 tacctacaac aacaactgaa gtcataattt tgtctgagcc ctcaggctct tcctttctca
    63541 catacatggc aggactctca cctgcaatta tatcttacaa tttattataa taaaaaatcg
    63601 cctttttatc aagaagaaac aatagaaaga tttgctattt ccaactatta aaaaatgatt
    63661 tagaaaaatt actagctatt tactattatc tttccataaa agagtaaatt agttcggaaa
    63721 tttactaaaa ttacctcaac aagaaataaa cccccgattc attaccaatt tttcaagata
    63781 caaacgataa gcaacacgat aatggtaaac gataaaatcc ctacgacaac caatgccata
    63841 tctcactaaa taataaatta aaaatttaaa atgaacatgt tcccagtcaa aattatcacc
    63901 aaatgtagga ccatactctt cttcaatact atcatagaaa ttagccatct gctcataaat
    63961 tttttgtaac ataatcaaat actccttttc ttttttataa acttattcta acaaaaaatt
    64021 ttacacattc actatcaatt cctgaattgt taaaatacca gcctcaacaa gataaaaata
    64081 ggaaaagttg acaaaataga aacaaatttg ctacctaaat ttcaaacgat taaagttcta
    64141 aaaagaccgc cttaaaactt ttgggtaaat cctattagaa ctataatatt tttatatagg
    64201 tattatggtt ctgattttct agtataaaaa attcaaacta gaattcttct tagaaaaggc
    64261 cgttttaagt gctatcactg ttcaaaaatg agggtcgctg aaacttctat cgtcaagaag
    64321 aatcatcaaa ttccttgtat catcaaccaa aagattgctc agaagctaat taaaaagact
    64381 tctatgaccg acattgatca tcagttgtct atttcaactt caactgtcat tcgcaagatc
    64441 aatgattttc actttgagca tgatttttcg cgtcttcctg agattatgtc ctaggacgtt
    64501 gaaacagtcc ggggagtgac tgtttcaatc gggagatgga gatgagcttt attgctcaag
    64561 actttgataa gctcaatatc ataactgttc ttgagagcag aacacaagcc atcatccgaa
    64621 atcccatgaa tacaaggcta tcaagcgata ctgaaagctc attcaacaag atagtcagaa
    64681 actgagtgat aaacgatttt atcgccctac ttttcgtatg cacttgacta ataaagaaat
    64741 tctagataaa cttttgagct attccgaaga cttgaaacac cactataatc tctatcagct
    64801 cttgcttttc cacctctttt tcagactctg tttaaaacct tcctcaaaga taaagaaaaa
    64861 atcgtcaacg gccttcaatt accttattcc aacgccaaac tggaagccac caataatctc
    64921 atcaaactta tcaagtacaa tgcctttggt tttaggaact ttgaaaactt caaaaaagaa
    64981 aggacgaaat ttgtcctttc tcaagcttag cttttcttca acccactaca gttgacaaag
    65041 agccactatt tttggttcat tttactataa cacgaaatag attattatta cataataaaa
    65101 aagaggtcta aacctcttaa ctaaattact ccgccagtag gactcgaacc tacgacatca
    65161 tgattaacag tcatgcgcta ctaccaactg agctatggcg gatta
//
LOCUS       DAVKWS010000005        64182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKWS010000005 DAVKWS010000000
VERSION     DAVKWS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.005"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            275..451
                     /locus_tag="VW852_000365"
                     /pseudo
     CDS             275..451
                     /locus_tag="VW852_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            785..925
                     /locus_tag="VW852_000366"
     CDS             785..925
                     /locus_tag="VW852_000366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271558.1"
                     /translation="MIDATEVQINRPKKELANDSGKKKCHAMKAQAIVTSQGRLFLWI
                     SL"
     gene            910..1254
                     /locus_tag="VW852_000367"
     CDS             910..1254
                     /locus_tag="VW852_000367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV0271559.1"
                     /translation="MDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQVQ
                     TPRKSSKLKPLTAENKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMN
                     LSAGIINHELGF"
     gene            complement(1417..1668)
                     /locus_tag="VW852_000368"
     CDS             complement(1417..1668)
                     /locus_tag="VW852_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271560.1"
                     /translation="MPKTKTLAELADVILWSFDFVNDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(2045..3925)
                     /locus_tag="VW852_000369"
     CDS             complement(2045..3925)
                     /locus_tag="VW852_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0271561.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAVYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSTFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEFYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3919..4788)
                     /locus_tag="VW852_000370"
     CDS             complement(3919..4788)
                     /locus_tag="VW852_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0271562.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALISYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4870..7515)
                     /locus_tag="VW852_000371"
     CDS             complement(4870..7515)
                     /locus_tag="VW852_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV0271563.1"
                     /translation="MENIVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(7606..8886)
                     /locus_tag="VW852_000372"
     CDS             complement(7606..8886)
                     /locus_tag="VW852_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV0271564.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            9057..11141
                     /locus_tag="VW852_000373"
     CDS             9057..11141
                     /locus_tag="VW852_000373"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV0271565.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPLGMNYFVPQTSD
                     QGGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11181..12860)
                     /locus_tag="VW852_000374"
     CDS             complement(11181..12860)
                     /locus_tag="VW852_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV0271566.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSSWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGFPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13615..14844
                     /gene="arcA"
                     /locus_tag="VW852_000375"
     CDS             13615..14844
                     /gene="arcA"
                     /locus_tag="VW852_000375"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV0271567.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPVYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14905..15918
                     /gene="argF"
                     /locus_tag="VW852_000376"
     CDS             14905..15918
                     /gene="argF"
                     /locus_tag="VW852_000376"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV0271568.1"
                     /translation="MNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYL
                     AGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGARVLITEDA
                     DEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAF
                     HDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLYI
                     PKV"
     gene            16083..17030
                     /gene="arcC"
                     /locus_tag="VW852_000377"
     CDS             16083..17030
                     /gene="arcC"
                     /locus_tag="VW852_000377"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV0271569.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVSQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17241..18752
                     /locus_tag="VW852_000378"
     CDS             17241..18752
                     /locus_tag="VW852_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV0271570.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18774..20105
                     /locus_tag="VW852_000379"
     CDS             18774..20105
                     /locus_tag="VW852_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV0271571.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKNASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(20492..20782)
                     /locus_tag="VW852_000380"
     CDS             complement(20492..20782)
                     /locus_tag="VW852_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271572.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(20770..21021)
                     /locus_tag="VW852_000381"
     CDS             complement(20770..21021)
                     /locus_tag="VW852_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0271573.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(21375..22259)
                     /locus_tag="VW852_000382"
     CDS             complement(21375..22259)
                     /locus_tag="VW852_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0271574.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(22404..23555)
                     /locus_tag="VW852_000383"
     CDS             complement(22404..23555)
                     /locus_tag="VW852_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV0271575.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(23701..25467)
                     /locus_tag="VW852_000384"
     CDS             complement(23701..25467)
                     /locus_tag="VW852_000384"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV0271576.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPIMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(25480..26208)
                     /locus_tag="VW852_000385"
     CDS             complement(25480..26208)
                     /locus_tag="VW852_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV0271577.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYRPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            complement(26223..29240)
                     /locus_tag="VW852_000386"
     CDS             complement(26223..29240)
                     /locus_tag="VW852_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEV0271578.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYFSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            complement(29251..31467)
                     /locus_tag="VW852_000387"
     CDS             complement(29251..31467)
                     /locus_tag="VW852_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV0271579.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQDFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLGTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            complement(31489..33360)
                     /locus_tag="VW852_000388"
     CDS             complement(31489..33360)
                     /locus_tag="VW852_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEV0271580.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKRMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTVVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            complement(33366..34685)
                     /locus_tag="VW852_000389"
     CDS             complement(33366..34685)
                     /locus_tag="VW852_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV0271581.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGTSKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(34682..35521)
                     /locus_tag="VW852_000390"
     CDS             complement(34682..35521)
                     /locus_tag="VW852_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0271582.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            complement(35524..36453)
                     /locus_tag="VW852_000391"
     CDS             complement(35524..36453)
                     /locus_tag="VW852_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0271583.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
     gene            complement(36540..37832)
                     /locus_tag="VW852_000392"
     CDS             complement(36540..37832)
                     /locus_tag="VW852_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271584.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVQTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            complement(37861..39264)
                     /locus_tag="VW852_000393"
     CDS             complement(37861..39264)
                     /locus_tag="VW852_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV0271585.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGLLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEGTAVGNIVVQLLSQGKINEDTELK
                     DIMTNIAETQYYYPQLS"
     gene            39440..40213
                     /locus_tag="VW852_000394"
     CDS             39440..40213
                     /locus_tag="VW852_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0271586.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(41342..42847)
                     /gene="adcA"
                     /locus_tag="VW852_000395"
     CDS             complement(41342..42847)
                     /gene="adcA"
                     /locus_tag="VW852_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV0271587.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(42857..43663)
                     /locus_tag="VW852_000396"
     CDS             complement(42857..43663)
                     /locus_tag="VW852_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0271588.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(43656..44360)
                     /locus_tag="VW852_000397"
     CDS             complement(43656..44360)
                     /locus_tag="VW852_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0271589.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(44360..44800)
                     /gene="adcR"
                     /locus_tag="VW852_000398"
     CDS             complement(44360..44800)
                     /gene="adcR"
                     /locus_tag="VW852_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV0271590.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(44955..46223)
                     /gene="dltD"
                     /locus_tag="VW852_000399"
     CDS             complement(44955..46223)
                     /gene="dltD"
                     /locus_tag="VW852_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV0271591.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(46216..46455)
                     /gene="dltC"
                     /locus_tag="VW852_000400"
     CDS             complement(46216..46455)
                     /gene="dltC"
                     /locus_tag="VW852_000400"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV0271592.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(46469..47713)
                     /gene="dltB"
                     /locus_tag="VW852_000401"
     CDS             complement(46469..47713)
                     /gene="dltB"
                     /locus_tag="VW852_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV0271593.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(47710..49260)
                     /gene="dltA"
                     /locus_tag="VW852_000402"
     CDS             complement(47710..49260)
                     /gene="dltA"
                     /locus_tag="VW852_000402"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV0271594.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMSGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(49281..49412)
                     /locus_tag="VW852_000403"
     CDS             complement(49281..49412)
                     /locus_tag="VW852_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV0271595.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(49726..50904)
                     /locus_tag="VW852_000404"
     CDS             complement(49726..50904)
                     /locus_tag="VW852_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0271596.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(51361..52638)
                     /gene="ltrA"
                     /locus_tag="VW852_000405"
     CDS             complement(51361..52638)
                     /gene="ltrA"
                     /locus_tag="VW852_000405"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV0271597.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEITKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKFLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRHKTLVGTPQGGNLSPLLSNVMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSASRFIEKRLGLKVNMTKAKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     RIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            53726..54070
                     /locus_tag="VW852_000406"
     CDS             53726..54070
                     /locus_tag="VW852_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271598.1"
                     /translation="MSKMFKCMCLLVMSVFCMSSISPSLVVFADDSLSANSKVVSGEA
                     QFENGSSVRFGDTQVNILSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEID
                     LNISFRSYDPNV"
     gene            54125..54385
                     /locus_tag="VW852_000407"
     CDS             54125..54385
                     /locus_tag="VW852_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271599.1"
                     /translation="MTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(54472..54618)
                     /locus_tag="VW852_000408"
     CDS             complement(54472..54618)
                     /locus_tag="VW852_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271600.1"
                     /translation="MLVEFHKSQGTLETPEAQAEIAQKREEIEQRRAELEDKKQELLN
                     RLNK"
     gene            complement(54972..55676)
                     /locus_tag="VW852_000409"
     CDS             complement(54972..55676)
                     /locus_tag="VW852_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0271601.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(55744..57570)
                     /gene="glpO"
                     /locus_tag="VW852_000410"
     CDS             complement(55744..57570)
                     /gene="glpO"
                     /locus_tag="VW852_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV0271602.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTSAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANFYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKATLANNDLAELKN"
     gene            complement(57612..59120)
                     /gene="glpK"
                     /locus_tag="VW852_000411"
     CDS             complement(57612..59120)
                     /gene="glpK"
                     /locus_tag="VW852_000411"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV0271603.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEDAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(59279..60706)
                     /locus_tag="VW852_000412"
     CDS             complement(59279..60706)
                     /locus_tag="VW852_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0271604.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            60871..61743
                     /gene="hslO"
                     /locus_tag="VW852_000413"
     CDS             60871..61743
                     /gene="hslO"
                     /locus_tag="VW852_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV0271605.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            61730..62710
                     /gene="dusB"
                     /locus_tag="VW852_000414"
     CDS             61730..62710
                     /gene="dusB"
                     /locus_tag="VW852_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV0271606.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            62806..64154
                     /locus_tag="VW852_000415"
     CDS             join(62806..63247,63247..64154)
                     /locus_tag="VW852_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV0271607.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKWIDR
                     YGIEFGKKGKNRYYSPDLKQEMINKVLHEGWTKDRVSLEYGLPSRTILLNWLAQYRKN
                     GYTIVEKPRGRVPKMGRKPRTRPEERTELERLQAENEYLRAENAIPKKVKRTPIEGGK
                     RERRKTEIVQELMTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIE
                     HKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKGDVGKKAEN
                     LIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQV
                     QTMLEQSFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASISRKGNSPDNGMMES
                     FFGILKSEMFYGYEKTFKSLNRLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT        19253 a        13854 c        11630 g        19445 t
ORIGIN      
        1 gttataaaaa gttttattca atcattatct agtagaatag agtagtacat tttataaatt
       61 gtcatctctc cataaacaca gtatatcatt tttaaatttt aagtcaagga aaattaaccc
      121 actttaattt cttttttaac ccgtgttaaa aaagaaatta aagtgggtta aaaaagaaat
      181 taactgagta aaaactctcg aaattcaatt cgctatatta tcactcaact tgaacaaata
      241 ccagaaccac aaaaaacaga tgtaaaggaa aaatatggac ttaaaatttg aaggggttga
      301 tttagaatac aaaaaagcta aaaacaatct tccagagtct ttctgggaaa cctactcagc
      361 atttgcaaat acaaatggcg ggaaaataat tctcgggata gatgaaaaaa acattgacac
      421 ctaccaaaga gtcaatagac tccctgcgaa acaaaattat gaagcaagta aacaactaac
      481 tgatgcacga tttaagcgtc ttgttggtgt tcagcgtacc acttttaaag agatgttagc
      541 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag
      601 cctagaagac cttcttatgg ccactcttca atatgtgtga gaataccgca cttatgaaga
      661 aattgcggct gattttggta tccacgaaag caacttactc cgtcggagct aatgggttga
      721 agtaactctt gttcaaagtg gttttacgat ttcaagaact cctctcagtt ctgaggacac
      781 ggtaatgatt gatgcgacgg aagtacaaat caatcgccct aaaaaagaat tagcgaatga
      841 ttctggtaaa aagaaatgcc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
      901 attgtttctt tggatatcac tgtgaactat tgtcatgata tgaagttgtt caaaatgagt
      961 cgcagaaata tcggacaagc tggtaaaatc ttggctgaca gtggttatca agggctcatg
     1021 aagatatatc ctcaagtaca aactccacgt aaatccagca aactcaagcc gctaacagct
     1081 gaaaataaag cctataacca tgcgctatct aaggaaagaa gcaaggttga gaacatcttt
     1141 gccaaagtaa aaacgtttaa aatattttca acaacctatc gaaatcatcg taaacgcttc
     1201 ggattacgaa tgaatttgag tgctggtatt atcaatcatg aactaggatt ctagttttgc
     1261 aggaagtcta ttgaaatact aagcctacac agactgattt caattttacc attcttcata
     1321 gtcaaaacca tcataatctg gatattgttc tggcaacgtt ccaaccgaac gtacgagata
     1381 agcttcttcg tcctctgtct cgatttctct cagcacttag cattcaaaac gccattcatc
     1441 accgaagtcg aaaataaact taaatttttg tttgacactt aggctatcca gatagacatt
     1501 ttctatgtaa cgttcttcaa catcgtcact aacaaagcta agaaagtaag aatctgcatg
     1561 actccactca acattatcca tgaaaaatgc atgagcatga tcatttacaa aatcgaaact
     1621 ccataaaatc acatctgcta gttccgctaa cgtttttgtt tttggaatct atatatgtcg
     1681 gtagcaacct tttttaattt gacggaaatg atccaagctt ctacagatgc tggcttttca
     1741 gcttctattt cttgttttac taattttaag tgtagcataa tacctccaat tgctcatcga
     1801 tagaattgct cttaggaaac attcactttc tgctatttta tcataagttt gtctaactgt
     1861 agcatttgaa accagataat tcttctgttt cacgcgaaac accctgcgcc aagcaaggtg
     1921 ttcttgtttt tatactcaat gaaaatcaaa gagcaaacta ggaagctagc tgcaggctgc
     1981 tcaaaacact gttttgaggt tgtagatgga agctgacgtg gtttgaagag attttcgaag
     2041 agtattaagt cgtataaatc gtcgacgctg tcgcaatggt atgccactgg ttggctgtag
     2101 ttgctgcgaa atccttgtct gcgtagaagt cagtaaatgg taggatttca acttccagct
     2161 cgtcgatgcg gtcaagctga ccagccagat aaaactcgat acggctttct gctcgtttga
     2221 tgcgttgcaa gagtccgccc atacggatgt caactgtatc caaaccaaag accttgtttt
     2281 ctttcagcca ttggtggcta aagagggcat ggaagtcttc aatttggctt ctaagttctg
     2341 gtaattcttg tctggcgatt tgttgtaaac tttctttatc atccgcttgg taggcctgac
     2401 gaatgcgtcg tcccacatct actttgctac ttaaaatagc attcaactgg gcctgagttt
     2461 caaagagata agcatagttt ccagcttttt ctttaatgtt agcaagcgtc tcagcagcct
     2521 gagcgaagtg cggtttgtcc tgttcaggtg tcatgtgttg atcaagaatc ggacaaagaa
     2581 tatcctgata aaaaacatag cggttgggat tgataccgct gagattgcct ggtaggtctg
     2641 gtaagaggtt ggcaaggtca atctgcataa aatcctcaac cgttagacca gtattggtct
     2701 tgaagtgcgc agacaaacca tctaggtcat ggcgatagct gagttctgcc cagatttgca
     2761 agcttggtag gatagagaac tgggcagttt caccaccatt gtctccccaa cccgttacga
     2821 tgacttcttt aatctgattg gcacggcagg ctttattagc ctcgatagcc actagacggc
     2881 taaaatggtt gtgaggtgta aagccaatcc acttccaagc tccccctgca aatgcaaggt
     2941 catggctaat cttgtgatga ttgcggaaat tacggttgta tttttcctcg ctatcctgat
     3001 aataatccca gtaaaccaga gtcacacggt ctttgagacg gtctaggtag acacgagttt
     3061 cctctggaat ttccacatca cgatcgtact ggccatccgc tgacatgagt ttgaagaaca
     3121 tatcactcca catctggcag tggaaaccat atttgtcagc aatatccagc acgcgctcca
     3181 agtgttggca catgaggaga ctacgatcca caacaccgtt caaaatcagg tagcgtccca
     3241 aaccaaccaa gtgggcttcg tccatcccga tattgacctt gcgagtcttc agtttagaca
     3301 acgtggcaaa catgccatca atcaggtcat aaaccttttc ttcgccaatg agaagaatgt
     3361 cctctacatc acggagctcc tgcacttcct tgacacccca tttgacaaag gtcgacaagt
     3421 gggccaaggt ctggatgcat ggtacaaagg tcatgtcaaa ctgttgagca taggcttcga
     3481 tttcctgcaa ctcctctgct gaataagctc cacggaaata gccaaagtaa ggctgccctt
     3541 caatctggta agtgtcttcc atgtaaagct caaaggttga gtagcccatg agagccaaga
     3601 cctctatcat ctgcttggca gaagccacat tcagcaccgc atttcgagaa cagtcaacca
     3661 tgtaagccaa atcttcgtaa actgcttgtt cctcaatctc tactttatca gcttctgcta
     3721 gaactgttac caacaaggat aaggcacgat aaagttggtg aggtttgcgg taggtcaatt
     3781 gatagtgacc ttcctcaccc tcgatagaga tagaggcttg gtcagactga gtgacagcca
     3841 cttccacatc tggtagagaa atatgacctt ttaaaacctc aatagcttgc gtttgtttgg
     3901 gactaagtcc tgtaaatctt accattgctt ttcctcctgt agccagttga caagagcacc
     3961 gtagagattg gcatctgcgt gataggtgca ggcctggata actggtgcga ccgtgtattc
     4021 ttcgtaggta tcgacaaagt tatcaacagc cttcttgaca tcttggataa aatctggatt
     4081 ttgactgata gagccaccca gactgatgac atctggatcg atgagatact ggatattgag
     4141 caagccttgc gccagattgc ggttcatgcg ctcaatggct tcttgacaaa gagcattacc
     4201 agctgcggcc tcttggtaaa tcttgcgacc gtcccaatca gtatgaccag atttttcaat
     4261 cacgtatcgt accatattcc cagttgatgc tagttgcgac cagttattaa gtttttcagc
     4321 aggggcaagg gttgtcatgt agccaaattc tccacccaag ccgtggcgac ctcgatgaag
     4381 tctaccattg ataatcatgg ctccgccaat ccctgtccca atcacgacac aggctgcatt
     4441 ttcaagatct ggatgagcta gtagttcact gagtccaacg cagttggcat cattttctaa
     4501 atggacaggt agctgataag agataagcgc ctcataccaa gaaaagccat ggatgtaggg
     4561 caccgcactg aagccatcaa tcacacctgt ctcttgattg actgcacctg gaacgctcat
     4621 agcaatccca ctgtaatcct gttctgacaa gcgttgatct agccacgcta gtaaatcctc
     4681 caagttttca ggcgttgaaa tacttgtctt atccagtatt ttcccatcag gagtcagact
     4741 ggcaaactta atcccagtcc ctccgatatc aatcgttgca atggtcattg tttttaccat
     4801 aaaagaggcg aatcagcagt tagttcttac ttttcgcccc tcctttcttt tgatgtttac
     4861 tttttgaaat taaatttctt cttttttgat gaattctgta cgaatctctt gtggatccaa
     4921 tggtcctgaa tgaactggat atggtcgctc aagtaagtca agaatggttt gttgatgttc
     4981 tgatatgtgc acattttctt gactcatatt atagtaacgg aagacatagc cttcttcatt
     5041 ttccgccact ttaaaggctg ttggacaaac ttgcggtatg ctgagaacag aatggctcaa
     5101 gaggctacca gtcgcaacca cgcttccttc ctgtctagca agctgaaggc tggtaaacgg
     5161 tgtctgcaag gctttggcac gacggaaagc tgaaaagcgt tcttgggctt ggtggcattc
     5221 aagtgcaaac tcgacttcaa attcacgcaa gcattgtgct tctggcgttg ggaagtagcc
     5281 ccagtcacct agctcacctg atgcacgcaa aatggtcacg gcaatggtgt tatccccaag
     5341 gatttcgtat tcattcaatc ccttattgga tacagtcacc cctttttcat cgtcatacag
     5401 actgacaaaa gcttgttggt gttgaggatt ttcaggattt tcccatgaag cagctggttt
     5461 gtttggtcgt gtcaccacct catagatact ttcagaatca ttgcttggac gcgtgttatg
     5521 agtcttgacc aagagacgga tacggtgatc cttggcagtg ttagtaaagc gagtcttgaa
     5581 gcggatttgt ggattgtcaa cgaagacagt caactcagtt tccagaggaa tgtttgtcaa
     5641 ttcttctgac cgtccagcct cacgcttcat aaactcgatg ataccttgtt gctcttcttc
     5701 tagcttttca tccgcactga caggcacggt caattcatgt ttgagcaaga ttttagcata
     5761 gcgatctgtg ttttccaaga cctcgtggcc cttaagctct gcaaagattg gctctgttcc
     5821 ttttggttgg aaatagatat actcgtttcc gatgtcaccc cggtcttcga agcggataaa
     5881 atcttcatag gcttcgtgag ttgtcttatc atagactgtg atgttgtcat ccacactcac
     5941 cgttacgaat ggtgtatcaa tcactccgtt ttggtaaata ccctcacggt gttcttgttc
     6001 tccttccagc aattggaagg ttgtccaaga aagcggtgct aggtgaactg gaatggtcac
     6061 gcgcacttgt cgagcaatac gagcttggcg gaacttatct tttggtaaat cgtattcaaa
     6121 attagctccg aggtcttcga ttgtagcctc tacaggacga ccatccaagt cctccacacg
     6181 gtaacttggc aagataagag cagccatctt tttgtagcct tctgttgggt gcaattcctt
     6241 gaaatcacaa gtcgccacat caatcactgt gctgacagta tcgaccttat catgcaagcc
     6301 tgtgttaatg acagtaaaga gatagtcact ttgagcctta tccgtagcaa ttttaccctt
     6361 ccactcgttg agcaaattac ttttaacaaa gtttcctact tggttgacct tggcaaaacg
     6421 cgtttccatc tcgcggtgaa cttcgtccac gctacagcca cagatactat catgtggcgc
     6481 attctgcaaa agtgttttcc aagcataggt caactggtcc ttgtggttgt gtccaccagt
     6541 gataatagtc aagggttcta ccacttgctc taggaggttg ctattttctt ggaaggcttg
     6601 ttttaggtaa atacgggatg aagaagtgtt ggcaagtgtg taccagccat ctgtttcctg
     6661 actggtcaac tcgcctgtaa cagttgataa gtgttcagga agcgcacctt ctacagcttg
     6721 aacatattca tcaaaagaac tatgaacaaa gattacatcc gggaagagtt catttgccac
     6781 acgaatggct tcgctcagat ttttctgtac aggctggtgg tcacagccgt tcatcatcaa
     6841 ccattggttg gtcgaagcgt aggcacgcac atctgacaat ttttgtttcc agaaggtcaa
     6901 ggcctcatct ttgtcaactg gaatttcatt cccgttactg taccagttgg caaagagaat
     6961 acctaaaaca cgactaccat ccacaccctg ccagtacatt tctgaaaact gggatgtaaa
     7021 ctgctcatct tcaaggactt ggttgtcaaa tccaatcggc ttcacaccac gaccaaaggt
     7081 cgccacgtga atgcctgatt tttgaagaat ttgaggcgct tgtcccatat ttccaaaggt
     7141 atctggaaag tagccaatct gggttgattt accccatttg gcagcttctt gttgaccaat
     7201 caaggtattg cggacattgg cttcactgga gatcaagtag tcatcctgca agatgtaaaa
     7261 gggaccaatt ttaagtttgc cctcgtcaat gtagcgttgg accttgtcac gattttcagg
     7321 gcgaatttgt aagtagtcgt caaggacaat agtttgtcca tccaagtgga aactcttgaa
     7381 ctcagggtca ttttcaaaga gatcaaagag attgtcaaac aattccacca actgcatacg
     7441 atggctttca aaaggcaagt accactcacg gtcccagtga ctatgtgaga taatatgtac
     7501 aacaatattt tccatgaagt aaaacctcat tctaaattta aattttaacg ttttaaatgt
     7561 aaacttgtga ttctgataag aatcggttga gctaaagcga gccccttagc gaatatccaa
     7621 gtaatctaag accaactcac agaacatcat gttagcccaa gagaaccatt cacgagagta
     7681 gagggtcgga tcatctacgt ggaagctttc gtgcatgaca cctgtaccac catcgcaggc
     7741 aaccagctga tccagcaaga attttttctc tgccttatct cttgttgtca atccttggat
     7801 agaaagggcg attggccaga tatagcgata gaaggtatga gaacttccta gaccgctagc
     7861 gtattctcct tggtagaaat atggattttc agagctcaaa atggtacgac gagtagcttg
     7921 atacacttca tcatcgaccg aacagtagcc cagatagggc gcagctagta gacttggtac
     7981 atttggatca tccatgatgc tggcatttcc taggccatcc acttcaaaag cgtaaatctt
     8041 ttcgcccttg ctgttggtgg tgtaagcgta gtttttgatt ccttcttgga tttcatcctg
     8101 aagacgcttg gcatcagcaa taacactctg gctatcagct aggtttaatg ctgcgaagat
     8161 ttcttgcaca taacccaaga ctactacagc aaacatattt gacggaatca agtaactata
     8221 ctgacagcag tcatcgctcg gacgaaaggc tgaccaagtc atacctgtca ctgcaaagtc
     8281 aggtccaaag ccatcattta ccaaggtgtc ttccttacgg tccgtatctc ggacaaaacg
     8341 ataaggagag ttcttgtggt cttgttccac cgtccacaga tggagaattt ccttagtcgc
     8401 tgcgacaaaa gtctcatcaa actgaccagt ctcgccagtc tctttccaga ggagataagc
     8461 caactgcaaa ggatagcaaa gcgaatccac ctcatacttg cgctcccaga tccagccgtt
     8521 aaggtctgtg tggtcagtct cgtggtgccc tttccagttc tcctcaatgt tgaaggagtt
     8581 agcataggga tccttgagta ccaaggtcat ctgacgtttg accaaacctg caatggtctg
     8641 acgcaggagg gcatctcttt tagctacatg aaggtagggt ctgagttggg ctgtcgaatc
     8701 tcgaagccac atggcaggaa tatccccagt caagacaaaa gttgaaccat cttctaagat
     8761 ttcaaccgta ttgtccaagg tatcggtgta gcaacgctcg aaaacatcca cccactctgg
     8821 atggtcctta gcccgctctg ctacttcatc tagccattct ctaacaattt ctttcgaata
     8881 aatcatcgtg caattccctc tttcaaacaa gtttcatgtt cagtatatag cttttgagac
     8941 agaaaataca tacacaaatg atagtcattt ttctacaaaa taattatttt tgaaactcct
     9001 tttctaaagg ccctcttttt ctgatataat gtagaaaaac agctaaagga aagactatga
     9061 aaccactact tgaaaccatc gatacccgct ttggtactgc tagcaagcat gccttctctc
     9121 ggggaaatac cctgccatac acaggcgtgc ctttggggat gaattacttt gtgccccaga
     9181 ccagtgacca gggtggatct tggttttttg atccgcatct gcctatcttt caggggattc
     9241 gattaactca ccagcccagt ccttggattg gggactactc ttggctcctt ctgacacctg
     9301 tcaccagtca actgggggga gacagcctct ttcaccgcca gtcttcttat gatatagata
     9361 aagcttgctt ccagcctcac tatttgaagc tcttttctct gcgttatcag attgaaaccc
     9421 agctcacacc gacttgctat ggtgcttcta ttcgtttgaa tcaaaagcaa ggcaaagccc
     9481 tctccctcta tcttcacgca gcagatgaac tgacagtaga gcaagtagat aagcgaactc
     9541 ttgctctcag acaagaaggg aaaacagaga ccaataaaaa tccactgatg ctcttcactg
     9601 ccctgcaaat gaacacggat attcttgcta tcagccaaga agctggagac tggcgaattg
     9661 acttagcaag tagtcaaacc gagatgcaac tagccacttc tttcatctct ccttctcaag
     9721 ctctaatcaa tctacctcaa gaagattttg atagctgtaa aacaagtgcc caagcggatt
     9781 gggaaaatct cctccatcgt tttgacatta tagagacagg agaggctgac cgaaccttct
     9841 ttgaccactg cctctacaga ctcttcctct tcccacagac tttttatgag gttgatgagt
     9901 cagggcaagc catccacatg gatctggcta ctggtactgt caagcctggt gtcctcttta
     9961 gcaacaatgg tttctgggat accttccgca ccaccttccc cctctttgcc cttatcatac
    10021 cggagcacta tcaacgcttt ttagaaggtt tcctcaatag ctaccgcgat actggtttcc
    10081 ttccaaaatg gctggctcca gatgaacggg gtatgatgcc aggtacactt ttagacggta
    10141 ttatcgcaga tagcgcctgc aaggacatgg cccctgacct agaaggagaa ctcttccaag
    10201 ccatgctcga aacagccagc aaagccgacc ctctcggaat caatggccgc cacggactag
    10261 cccaatacca agaactaggt tacctctcta ccgaccacca cgaaagtgtt agccatactc
    10321 tagactatgc ctatagtgac ttttgtatcg ctagctgtgc caaaaaacta gagaacatag
    10381 aaatcgctga aacctacaag gctgcttcac aaaattaccg ccagctattt gacgctgaga
    10441 caggttacat gcgagcacga gacaatcaag gaaactttca ccctgacttc tctccttata
    10501 gttgggggcg agactacgct gaatgctctg ccattcaagc tactttaggc gttctccacg
    10561 acatccctgg cttaatccaa cttatgggtg gaaaagaaac ctttagcaac tatcttttga
    10621 aagcttgtca agatgctccc ctctttgaaa caacaggcta tggttatgaa attcacgaaa
    10681 tgagcgagat ggctactgct ccttttggac aaatagccat ttccaaccag cctagtttcc
    10741 acatccctta tctcttccgc tacagcgatt accctgacta cactgccctt cttatcaaga
    10801 cgctccgtca gaaagctttt cacccaagtt gggaagccta tcctggagat gaagacaatg
    10861 gtagtctctc tgcttggtac atctggtcag ctctcggatt ttatccaacc tgtccaggaa
    10921 aaccaagcta tgacctcgga atccctctct ttgaccatct acgagtctac ctagctaaag
    10981 aagataaatg gctggatatc catactaaac aaaaccacaa ccattttaac tttgtcaaag
    11041 aatgccgact gaacaaaaca ctcgtatcaa ctattcaaca ccaagacctc ttaaaagctg
    11101 aacaactaac tttcaccctc agctggctac caagtcacta ataaccaaaa gaacctttgc
    11161 atcgcgcaaa ggttcttctt ttatgaaact tggacttgaa gttgatccac aagctgacca
    11221 tccaccgtta ggttcagata gaaatccaag gttttatctc ctgcgaaata cagggttggt
    11281 agatgcaaat cttttttgct ttcccctgct tggaattgaa ggacataaat ctcgtcctga
    11341 taggcgacac catggacacc tgtccccggt tgaatcgaaa tcttagcttc taaaggacca
    11401 ctacattctc tgcgcttaac tgtaaaatag gcattttctc cctttgccac agctaggctt
    11461 ttttctgcaa atgctagttc ctgaacaact tctttttttg ataatccagg agttttataa
    11521 agggaaatct tggtcaatac aggaatagat tgtgcctcgg taatcatcac gcgcaccttt
    11581 tgtgcctcta ctagcggacc tcgcaagagg cgcttatgac caactgtgaa gcccctgcca
    11641 aattcctgcc agacaccatc cacctctact tgcacatgaa aagcagcgat tcgttgccct
    11701 agcttcaaat cttctcttaa ctcaattaca tcaaaagttt taggaaaacc taagtcgagt
    11761 tctaactgga tgggcaagtc tgcatcgctt gcccaagagc tggtctcaag gccgtctgtc
    11821 aaatggcgac aagcaaagtc tgcggaaaga gctggaccag atacctcagc tcccagagcc
    11881 aaatcttctt tatagagctc attgcgatag gtcgcaaatt cataaagtcg ttcaatatcc
    11941 tttgcatcaa agagcccagc ttgattcggc ggaatattaa gcaagagtgg agttcctcgc
    12001 cctactgagt gaaagtagat ttcgaccaac tcctcgagag acttaggatc ctgatcctca
    12061 tggtagaacc agcctggacg gatggaaaca tctgcctctc cgattgaaaa aatcgtgccc
    12121 gagggatccc cgtgctgaag atagtccagc tctgcttctg ttcctagttt atcaggattc
    12181 accttttgcc acagtggatc acctgcatac cctcgttcat tgccaatcca gcggatactg
    12241 gtgccttctg ttgaaaaaat caaacaatcg ccctgcaagt cacgaatggt ttcaaaccat
    12301 ttttcaaatt cataattaac cttttgcgcg ccctctcctc tggcaccatc catccaaacc
    12361 tcagcgaact taccagcatt cccatagtta ggatttgata agatttcctt caactgagcc
    12421 agataatagg cattgtagtc cgcttctcgg tccacatgat agaggggact atgggcatcc
    12481 cacgatgaca ggtagacccc catatccata tcaaactctg tggcagcttg ggatacttca
    12541 aggagcaagt cgccctttcc tctcctccaa ggactgacct taaccgaata atctgtgtga
    12601 gctgtcggat aaaggacaaa gccatcgtgg tgcttgacca ccaaaatcaa ctttttgaag
    12661 cccgtttcct tgagcacacg aacccactca cgcgcatcca actgactcgg gttaaagcgc
    12721 tcaggatcct cctgtccagt cccccattct tggtcataaa aggtattagg accgaagtgg
    12781 ataaaagctg ctagttcatc tcccagataa gctagctgag tctgacttgg taacggtcca
    12841 tgcggtttga ttttcttcat cgttatatat ctccttcttt agcaacttct cttgttcaca
    12901 aggttatcca caggttgtgg ataataaacc atgatctaac agccagctct ttccaactcc
    12961 aactggtagg ctcaggattt ttctcctcaa tattccctct tcaaacagct ctgtcatatg
    13021 gttctgctaa cacctctcag aatgctactc tccataatta ttctcaggct tctttcaggt
    13081 cattgtgtga agaccattca ctatccagac ttttccacag attgtgaata agtgattatt
    13141 ctaagctatc acctcgtcga ctataacaaa aaataaaaag agggtcaata agataattat
    13201 ctcttcctaa atactcagaa aagattctat aatttgtaca attgaacaag aaatgcttct
    13261 ccaaaatcta tcttcaaaag aatgttgcat ctttttatcc tttcacacaa acatttctct
    13321 ttatacagat ccaagtgata accagatgat aattggacac tttttggtca tttgtatatt
    13381 ttttgaaaaa ttttttaaaa aaattttata tcctttttat agaagtttat gcatttgtat
    13441 ctatgtattc tatcctatgt tatgtataat aacataaaat tgtgctaaac atcataattt
    13501 atattatttt ccgagaaatt atagccattg acattaccta ggaattgcgt tataacatac
    13561 atgtaagcgg tacccaaaat ggtgccaagt caaaattttt aaggaggaaa atacatgtct
    13621 tctcatccaa ttcaggtctt ctcagaaatt gggaaactga aaaaagttat gttgcaccgt
    13681 ccaggcaagg agttagaaaa cttgttgccg gactatcttg aaaggcttct ttttgatgat
    13741 attcctttct tggaagatgc tcaaaaagaa catgatgcat ttgcccaagc tcttcgcgat
    13801 gaaggaattg aggttctcta cctagaacaa ctcgctgctg aatcattgac ctctccagaa
    13861 atccgcgatc aatttatcga ggaatactta gacgaagcca acatccgtga tcgtcaaacc
    13921 aaggttgcta ttcgtgaatt acttcacggc atcaaggaca accaagaatt ggttgaaaaa
    13981 acaatggctg ggattcaaaa agttgaattg ccagaaattc ctgacgaagc taaagatcta
    14041 actgacttag ttgaatcaga gtatccattt gcaattgacc cgatgccaaa cctctatttc
    14101 actcgcgacc catttgcaac aattggaaac gccgtatcgc ttaaccacat gtttgcagac
    14161 actcgtaacc gtgaaacact ctacggtaag tatatcttca aataccaccc agtctatggc
    14221 ggaaaagtgg atttggtcta caaccgtgaa gaagatacgc gtatcgaagg tggagacgag
    14281 ctagttcttt ctaaagacgt ccttgcagta ggtatctctc aacgtacaga cgcagcttct
    14341 atcgaaaaac ttttggtcaa catcttcaag aaaaatgttg gcttcaagaa agttttggcc
    14401 tttgaatttg ctaacaaccg taaattcatg cacttggata ctgtcttcac tatggtagac
    14461 tatgacaagt tcactattca cccagaaatc gaaggcgacc ttcacgttta ctcagttact
    14521 tacgaaaacg aaaaacttaa aatcgttgaa gagaaaggtg acttagctga acttcttgct
    14581 caaaaccttg gtgtagaaaa agttcatttg attcgttgcg gtggtggcaa tatcgtagca
    14641 gctgcgcgtg aacaatggaa cgacggttct aacactttga ccatcgcacc tggtgtggta
    14701 gttgtttatg accgcaatac cgtgaccaat aagattttgg aagaatacgg gcttcgcttg
    14761 attaagattc gcggaagtga attggttcgg ggccgtggtg gacctcgttg tatgtctatg
    14821 ccatttgaac gtgaagaagt gtaatcgctg ttcggcattc gtcaatagaa aatgtaaaaa
    14881 atagaaagag gaaataataa aatgataaat tcagtattcc aaggacgcag cttcttagca
    14941 gaaaaagact ttacccgtgc agagttagaa taccttattg gtctttcagc tcacttgaaa
    15001 gatttgaaaa aacgcaatat tcaacaccac taccttgctg gcaagaatat cgctctccta
    15061 tttgaaaaaa catctactcg tactcgtgca gcctttacaa ctgcggctat cgaccttggt
    15121 gctcacccag aatacctcgg agcaaatgat attcagttgg gtaaaaaaga atctactgaa
    15181 gatactgcta aagtattggg acgtatgttt gacgggattg aattccgcgg attcagccaa
    15241 cgtatggttg aagaattggc agaattctca ggcgttccag tatggaacgg tctaactgac
    15301 gaatggcacc caactcaaat gctcgctgac tacttgactg ttcaagaaaa cttcggtcgc
    15361 ttggaaggct tgacattggt atactgtggt gatggacgta acaacgttgc caacagcttg
    15421 ctcgtaacag gtgctatcct tggtgtcaat gttcacatct tctcaccaaa agaactcttc
    15481 ccagaaaaag aaatcgttga attggcagaa ggatttgcta aagaaagtgg cgcacgtgtt
    15541 ctcatcactg aagatgctga tgaagcagtt aaagatgcag acgttcttta cacagacgtt
    15601 tgggtatcaa tgggtgaaga agacaaattc gcagaacgtg tagctcttct taaaccttac
    15661 caagtcaata tggacttagt taaaaaagca ggcaatgaaa acttgatctt cctacactgc
    15721 ttgccagcat ttcacgatac tcacactgtt tatggtaaag acgttgctga aaaatttggt
    15781 gtagaagaaa tggaagtaac agacgaagtc ttccgcagca agtacgctcg ccacttcgat
    15841 caagcagaaa accgtatgca cactatcaaa gctgttatgg ctgctacact tggtaacctt
    15901 tatattccta aagtataatt ttagataata aaccgtctac caacagctat gagggctgcg
    15961 actaatagct ttagtccggt cctcttttat gtaatggtaa tctattattt cttataaaat
    16021 atgtgaaaaa tcattaaatt gaaatctaaa cgcattctat tgagtgtgat aaaggagaat
    16081 ttatggcaaa tcgtaaaatt gtagtagctt tgggaggaaa tgcgattctt tcttctgacc
    16141 catcagcaaa ggctcaacaa gaagctttag ttgaaacagc taagcatctt gtaaaattga
    16201 ttaaaaatgg agatgatctg attatcactc acggtaatgg acctcaagtt gggaatctct
    16261 tgctccaaca tttggcatca gactctgaaa agaaccctgc attcccactc gactcacttg
    16321 tcgctatgac agaaggtagc atcggtttct ggttgaaaaa tgctttgcaa aatgctctct
    16381 tggatgaagg catcgaaaaa aatgttgcct ctgttgtaac gcaagttgtc gtagataaaa
    16441 atgatccggc ttttgttaac ttgagtaaac caatcggtcc tttctattca gaagaagaag
    16501 caaaagcaga agccgaaaag agcggagcga ctttcaagga agatgctggc cgtggctggc
    16561 gtaaggtcgt tgcctcacca aaacctgttg acatcaaaga aattgaaacc atccgtactc
    16621 ttttaaatag tggtcaagtc gtcgtagctg caggtggtgg cggtattccc gtcgtcaaag
    16681 aaaacaatgg acatttgact ggtgtcgaag cggttattga taaagacttc gcttcccaac
    16741 gtttggcaga attggttgat gcagacctct tcatcgtttt gacaggtgta gattatgtat
    16801 ttgttaacta caacaagcca aaccaggaaa aattggaaca tgtgaatgtt tcccagctgg
    16861 aagaatatat caaacaagat cagtttgcac caggtagcat gcttccaaaa gtagaagcag
    16921 ctatcgcttt tgtcaatggt cgtccagaag gaaaagcagt tattacttcc cttgaaaatc
    16981 taggcgcctt gattgaatct gaaagcggaa caattattga aaaaggataa gttgttttac
    17041 taataagata tattctattt ctagtatctt tatatcaaat tagaaattat tcttgaaaac
    17101 atgtacaata tttcaaaaga tattagtttt ttagacttta atatggtaaa acaaatataa
    17161 atagaaagcg ttttcttgaa tgtttattta agaaagtagt tggtttttta cactttgtta
    17221 gacatcagga ggaaaaacaa atgagtgaaa aagctaaaaa agggtttaag atgccttcat
    17281 cttacaccgt attattgata atcattgcta ttatggcagt gctaacttgg tttatccctg
    17341 cgggggcctt tatagaaggt atttacgaga ctcagcctca aaatccacaa gggatttggg
    17401 atgtcctcat ggcaccgatt cgggctatgc taggtactca tccagaggaa ggttcgctca
    17461 ttaaagaaac gagcgcagcg attgatgtag ccttcttcat ccttatggtt gggggtttcc
    17521 ttggcattgt caacaaaact ggtgctcttg acgtagggat tgcctctatc gtgaagaagt
    17581 ataagggccg cgaaaaaatg ttaattttgg tactgatgcc tttgtttgcc ctcggtggta
    17641 caacttatgg tatgggtgaa gaaacaatgg ccttctatcc actccttgtg ccagttatga
    17701 tggccgttgg ttttgatagc ctgactggtg ttgcaattat tttgctcggt tctcaaatcg
    17761 gctgtttggc atctactctg aatccatttg cgacaggtat tgcttcagcg actgcgggag
    17821 ttggtacagg ggacggtatc gtacttcgtc tgatcttctg ggttaccttg actgctctta
    17881 gtacttggtt tgtttaccgt tatgcggata agattcaaaa agatccgact aagtcactgg
    17941 tttatagtac tcgcaaagaa gatttgaaac actttaacgt agaagaatct tcatctgtag
    18001 aatctacact tagcagcaaa caaaaatcag ttctcttctt atttgtgttg acattcatct
    18061 tgatggtatt gagcttcatt ccatggacag accttggcgt taccattttt gatgacttta
    18121 atgcttggtt gactggtctt ccagttattg gtaatattgt cggttcatct acttctgcac
    18181 taggtacttg gtacttccca gaaggcgcaa tgctctttgc ctttatgggt atcctgattg
    18241 gtgttattta tggtcttaaa gaagataaga ttatctcttc cttcatgaat ggtgctgctg
    18301 acttgctcag tgttgccttg atcgtagcga ttgctcgtgg tattcaagtt atcatgaacg
    18361 acggtatgat taccgataca atcctcaact ggggtaaaga aggcttgagc ggtctatctt
    18421 cacaagtctt tatcgttgta acttatatct tctatctacc tatgtcattc ttgatcccat
    18481 cttcatctgg tcttgccagc gcaactatgg gtatcatggc tccacttgga gaatttgtaa
    18541 atgtccgtcc tagcttgatt atcactgctt accaatctgc ttcaggtgtc ttgaacttga
    18601 ttgcaccaac atctggtatt gtgatgggag ctcttgcact tggacgtatc aacattggta
    18661 cttggtggaa attcatgggc aaactcgtag tcgctattat tgtagtgacc atcgcccttc
    18721 ttctccttgg aaccttcctt ccattcctat aaaatagtga gtgaggtgat tccatgaaaa
    18781 tagatataac aaatcaagtt aaagatgaat ttcttatatc attaaaaacc ttgatttcct
    18841 atccttcagt actcaatgaa ggagaaaatg gaacaccttt tggacaagca atccaagatg
    18901 tcctagaaaa aactttagag atttgtcgag acataggttt cactacctat cttgacccta
    18961 aaggttatta cggatatgca gaaatcggtc agggagcaga gcttctggcc attctctgtc
    19021 atttggatgt tgttccatca ggtgatgaag cagattggca gacaccgcca tttgaagcaa
    19081 ctatcaaaga cggctgggta ttcggacgtg gtgtccaaga tgataaaggc ccttcgctcg
    19141 cagctctcta tgcagtaaaa agcttgctgg accaaggtat tcagttcaaa aagcgcgtac
    19201 gctttatctt tggtaccgat gaggaaaccc tctggcgctg catggcacgc tacaatacca
    19261 tcgaagaaca ggccagtatg ggctttgcac ctgactcatc ttttcctctg acctatgctg
    19321 aaaaagggct tctacaggtc aaacttcatg gccctggatc ggatcaacta gagcttgaag
    19381 taggaggcgc ctttaacgtt gtaccagaca aggccaacta ccaaggtctc ctctatgaac
    19441 aggtttgtaa cggtctcaaa gaagctggtt atgattacca aaccactgaa caaaccgtaa
    19501 cggttctcgg agtgccaaag catgctaaga atgctagtca aggtatcaat gctgtcatcc
    19561 gactagctac cattcttgct cctctccaag aacaccctgc tctcagcttt cttgcaacac
    19621 aagcaggtca agacggcaca ggaagacaaa tctttggtga tatagcagat gaaccttctg
    19681 gtcacctatc ctttaatgtc gcaggtctca tgatcaatca tgaacgttct gaaatccgta
    19741 ttgacattcg gactcctgtc ttagctgaca aggaagaact agtagagttg cttacaagat
    19801 gtgcacaaaa ctaccaactc cgctacgaag agtttgacta tctagcgcct ctatacgtcg
    19861 caaaagacag taaactcgtt agcacactga tgcaaatcta ccaagaaaag actggcgata
    19921 acagtcctgc tatttcatcc ggtggtgcca cttttgctcg caccatgcca aattgtgtag
    19981 ccttcggcgc cttattccca ggagcgaagc agacagaaca tcaggcaaat gaatgtgccg
    20041 ttctagaaga tttgtaccgt gctatggata tttatgccga agccgtctat cgacttgcaa
    20101 cttaatcagg caactgtttc tacaaaaaaa aatcgaccga ttaatgaact gcaccccaaa
    20161 agttagacag aataaatcta acttttgggg tgttttatta tgaaattgaa ttatgaagat
    20221 aaagttcaga tctatgaact aagaaagcaa ggacaaagct tcaaacagct ttcaaaaaga
    20281 tttatgtgga tgtttctggt ttaaagtcat ctgaatcttt gagatgagct ttataaatcg
    20341 cttttttcag tgtttgcact ggtgtttcga taaactcaaa ctttttagcc gtggtattgc
    20401 ctgattttat agtatattga aactagaata gtacacctct acttctaaaa catttttaga
    20461 aatcgatttg tcctgttctt atttcatttt actatatttg agccacttcg tctttaacgg
    20521 ctttattcat aagctcttgt aatttttctt tactatcaat tacttctgat tttccgttgt
    20581 aatttattgt aataggtttt aacttaccta atttctcgac acgctcatta atttgatctt
    20641 ttttgaaggc tgcatatgtt tttcctaaga ttttttcgaa aatatattta tcagatagcg
    20701 gtttgccttc ttcttcagct tggtttttgt attgatttga aacataagga acaaatcctt
    20761 catagtaacc taatgctccc ataagttcaa aagcttgttt tctaattcaa accattgcaa
    20821 ctcagatttc agcttttcag ataaatcctg ctcatccaaa taatgacttg aaattagtgc
    20881 tgaactcgtt tctgtatcct gtacaggctg atcacccata ccagcaaaaa ataaactcgt
    20941 tcctagcaag accgaacaag ctcctattgc atatttcctc aaagaaaaac gctgctttct
    21001 ctcaaattga aattctttca tcccatctcc catcattcat tattactgta tattttgtat
    21061 atcagaaata gtttgtattc acaaatcttt ctagttattc ccttatcatt cctaattaag
    21121 ggagataaca tacaataatt tttagttaaa tgtatatcga tgttttttgt ttttcttaat
    21181 aaacgcaata caaaaagagc ctgttgccaa gctctttgta ctcaatgaaa atcaaatagc
    21241 aaactaggaa actagccaca ggttgctcaa aacactgttt tgaggttgca gatagaactg
    21301 acttcgtcag taacatctat acggcaaggc gacgctgacg tggtttgaag agattttcga
    21361 agagtattag tctattattt cttctcagcg cgaagggctg acaagatttg tgttcggata
    21421 tcatccacac catttggagt atttggtaaa aagatagttt gatttccttt agaggcaaag
    21481 gtattcaagg tatccaaata ctggttggtc aagaggatag acatgatttg ttcttctgtc
    21541 atgccaacat tggcttcctt gagttcggtg atagactctg ccaatccatc cacaatcgcc
    21601 ttacgttgtt gggcaatccc cacaccatga aggcggtctt tttctgcttc tgcttcagct
    21661 gcagtgacaa ttttaatctt gtcagcttcc gccaattctt gtgctgcgac ccgcttacgt
    21721 tgcgccgcat tgatttcatt catggattgc ttaacttctg catccggttc gaccttggta
    21781 atcaaggttt tcacgataat gtagccgtaa gtggtcattt cttctgctac ttggtgttga
    21841 acttcaaggg caatctcatc ttttttctca aacaattcat ccaaggttaa ttttggaaca
    21901 gaagagcgaa gagcatcttc gatataagat ttaatctgag attctggacg tatgagttta
    21961 tagtaagcat ctgtcacgct ctgctcgttg acacggtact gagtcgctac attcatcata
    22021 acgaacacat tgtccttggt cttagtctca accacaatat cactttgcaa caagcgcaac
    22081 tgaatccgtg ctgcaatcga gtcaatccca aaaggcaagc gaatatgaat accgctatta
    22141 gcaacctttt ggtatttccc aaagcgttca ataatcgcca ccgactgctg acgaaccaca
    22201 taaactgtac tcagtgtgac tatcaccaat aggagcacac aaacaatcag aaaaatcatg
    22261 aaaaatattg ccataatgga acctccacaa gtatttttct agtattatag cacatttaaa
    22321 gaaggctgtg ccgtttttac tgcgattttt cctgaaatgt caataattag aggtgaattg
    22381 tcctattgtc gtccaatctc ttgctaaaat aactctttat aaaaggcaat cgtttcttct
    22441 aaggttggca taaatggatt tcctggtgcg caggcatcaa tcaaggcatt cttagaaagg
    22501 tattcaaagt cgaaatcttt ttcttcaata ccaagttcag tcagtttctt aggaatacct
    22561 actgtctcag aaagcttctc aatctcagca atcgcataat cggcacattc ttgatctgat
    22621 ttaccttcta catgaagtcc caaggctttg gcaacattgc ggaaagcttc tggtacacgt
    22681 ttagcatttt cacgttctat aactggtagc aacatggcac agcaaacacc atgtggcaaa
    22741 ttatatactg caccgagttg gtgagccatt gagtgaacat agccaagacc agcattatta
    22801 aagctcatac caccaaggaa gatggcattg accatacctt cacgcgcttc aatatcatgt
    22861 ccgtttgcta cagcacgagg aagatattcc ttgataagct caatagcacc aatagacagt
    22921 ttcttggtta catcataagc accaggtgtt accaaagctt caacagcgtg agtcagagca
    22981 tccataccag tagcagcagt caggccttta ggtttggaaa gcatgagttc cgggtcatta
    23041 acagagataa gagcaaggct attcttgtca accattacca tcttaacctt gcgttcttca
    23101 tcagtaatca catagttaat agtgatttct gcagaagttc ctgccgttgt attgatagcc
    23161 acaactggca agcctttttt agcagactta tgaagccctt cataatcctg tggttttcca
    23221 ccatttgtag ccatgataga aatacaacta gctgcatcct gtggagaacc cccgccaaga
    23281 ctgatgataa agtcacagcc atgttcttgc aaagcagcta ccccatctgt gacattttta
    23341 caagtagggt ttggctctac atcgctaaag atgacatatt cgattccttc tgtatccagt
    23401 ggttttaaaa ccttaggtaa aatatcactg ccttcgatgt acttatctgt aaccaaaagt
    23461 gcctttttat agccaagttc cttgatataa ggacccactt catttacaac accttttcca
    23521 ataaggttga ctgccggaac ataaaatgta gccatataat tttcctcctg cgcctccgcg
    23581 ctatctattt aacatcatta tattactttt tgtattcgtt ttcaattatt tgtgaattgt
    23641 ttaacaaatc tttttaaaag gataggaggg acgtaagttc acctcctata taaacaagtt
    23701 ttatttatgt agtggtccca ataactgaca tgcacgatag tctgctgatt ctagatcttc
    23761 tgttccaaat aaggaccaat ttttaggtct aaagatatct tcttcgggta cattatgcat
    23821 attgacagga attctcaaca tagaagccaa ggtaatcaag tctgctccaa tgtgtccata
    23881 tgttatggct ccgtgattag ctccccaatt attcatgacg tcatagacag acttgaaagc
    23941 accttttcct gtcaaacgtg gagcaaacca agtagttggc catcctggat ctgtacgatt
    24001 atctaaagta tggtgaacat cttcaggaag ttcaagtgtg taaccttctg caatttgtag
    24061 cactggacca acccctttta gaagattgag acgtaccatt gttactggca tatccccctt
    24121 cgtcaagaaa cgagttgaga atcctcctcc acggaagtat tcgcggtttg ctggtgggaa
    24181 gtctgtattt tcaagcatag cctgcacttc actttcttcc aactcccaga atggtttcat
    24241 aataggtttg ccatctcgag tagcttgacc tgtaccatcc aatgtacaag aaccagagtt
    24301 gattagatgt aagaagccag ctgcagcacg accctctaaa gtatgtcccg ttacacgttt
    24361 aacagcctct gggctccaat aagtacgcac atcagcaaag atttgtggag tatttgttaa
    24421 tagataatta aagagcatag acacaccatt tagtgaatca ttctctgtcg caaatacaaa
    24481 tggttttcga ataccattcc agtcaaactg agtattgagg aaagtttcca taaagtcacc
    24541 atttggaaaa tggtctgtcc actggcgttg accttggaaa ccagctacta aagcatggtg
    24601 accaactgct tcttcctcaa aaccaagttc agcaagtctt gggttaccaa ccattaagtc
    24661 acgtccaatc atgaacatct taataacaaa ttcccattgt ctatcttttt cttcacggct
    24721 taaaacaagg tcttcacggt tatggtcgaa tccttctttt acgttttctt tcacccattt
    24781 gagcgcacgt tcgaactctt cagggtcgta aataccacgg tccatacggc gtgtgaactc
    24841 cgtcatatct accgattcat ttcgcattcc taagtattct tggaagaaat ctggatttac
    24901 aatagaacca ccaatcccca tcgaaacact acccattgat aggtaagcag tgtctctcat
    24961 caagccagtt gcaagagctg cacgcgcata acgtaaaagt ttttctttga catcttctgg
    25021 aatatctgta tcattagctt cctgaacatc tctaccataa atcccaaagg ctggaatccc
    25081 tttttgagca tgtgaagcta gtacagctgc aagatagaca gctcctgggc gttctgtccc
    25141 attaaatccc caaatagcat gaggaatatc tggagacata tccatagttt cactaccata
    25201 acaccagcat ggtgtaactg taattgttgc gcaaacattt gattttttaa acaactcatg
    25261 ggaagctgca gcctctggaa cacgaccaat ggtagatgga gaaatcacac attccacagg
    25321 ttccccatct ggatatttca atgtgcttga aatcaaatct gccacacttt tagccatgtt
    25381 cattgtttgc acttcaagtg attcgcgtac accttgacga cgaccatcaa tagtcggacg
    25441 aataccaata cgtggatgtt gaatcatact attttcctct tatttttctg tttctttcat
    25501 actaccatct ccattatatt tacggtagcc tgggtgacga cctgtaacct tataattttc
    25561 tcgcattgag aatagacgtt ctaaagtcgg acgagcaatt tcttgctcct caatgccctt
    25621 tgtagaaagt aacattcttc cgtggaatgt tgtctttgcg actaattcta aagtttccat
    25681 acggtagtat gctgtaatga catcgcttcc gacagtcaga gctccatgat tttctaatag
    25741 catgacatca tgatcgggca gataaggtgt aattgcttct ggcacttcca ttgtagacgg
    25801 aactccaaat ggcgttatag gaattgcccc taccacaatc gcgctctcaa ttagtgaata
    25861 agtatctaaa ggaatgtgtg caagagcaaa tcctgttgca atcggtggat gcgcgtgaac
    25921 aactgaacgg acatcctcac gttcttcgta acaacgaatg tgcattttaa tttcactaga
    25981 gggccggtaa tcaccttctg cttctaaaat ctctcctttg agatttaact tcaccagctt
    26041 ttctggagta ataaaacttt tgctgatacc agtaggtgtt gcaagaatgg tatcctcatc
    26101 taatcgaaca gatacattcc catcgttagc agcaacccaa ccaagttgcc acatcttatg
    26161 acaaacatca caaatctgtt cacgaattaa tttttcatct tgagacataa actcactcct
    26221 ttctaaaaat gaagttcgaa ttcaagattt ccgttactgt taatggtaat agtaaccata
    26281 tgatttttct gatcaaaatt gagagatggc tgatcatagg aattatttgt tcctgataat
    26341 gaatgaatag taggaagttt atctatacct cttaatacta tctttgtaac ccttttctct
    26401 ccaaactgag tatctttgat ataatgttct tcaatccact taatcgcacc tttcttggtc
    26461 aattgtggta gtttctttgc ctgatttgca tcttgagcat tagaccatag tgaatcttta
    26521 tttgttctaa aattatttaa attcactttc aatccattac ttattctctc aaaaatacca
    26581 taggtatgag gttcaaattc aacatccatt tctaaagaat taaattttaa tttcccagtc
    26641 tgtttaacat tctcatttac cttataatta tagacaaacc atctattatc taatttctga
    26701 gcaaagatat ctccttcata attcatagga tacagcttat ttaagtattc ttttctagcc
    26761 tgagtagagc taaattctgg agaagtgatt tccttaatct gaatacggct accagaaaca
    26821 gactctttca atttatctgt ttttaaaaca cctggaatag ctggaataac attataacga
    26881 ccagtcatgt ataatggagt ttgttctcta tcagtattta cgtttacaaa gaacttacca
    26941 tttcctttat ttgaatagtc tccatgtatg aatacttttg tatcctcaag taccttttct
    27001 ttggatggtg ctggatgcgc tattacatat ctaaaaaatt cttttattac ttcgctaaag
    27061 agtaagcttt ctttattatt tacaccatag gtataagctg ggtgttcaaa attataaact
    27121 acacctccat taaggtaaac ccctaaagct tcttctccta gcatagactc tggttcagtc
    27181 aaccattgtc tatctccttg actttttccg atattaccac ttgcaaacag tttccattta
    27241 cctgtctcat accacttcca agtatccatc aaaccacccc attgataagt atgatttgaa
    27301 agccaaagtc ccttcatata actttctgta gttgaatcat ttccctcagc agcaggtgta
    27361 tttttgaaca tgaaaataag gttcttccag tacttatcaa cagatttctg aaatgctatc
    27421 ttaccgtttt ttccaaatgc tttttctatt gcagaaccat tattttgttc agcccatata
    27481 aaatatcctc catttttagc tgatactttt aagtaatctg ccgccttatt ttcaatatca
    27541 tttgcccaaa tccaataatt ctcagtactg aaaataccat gcaaatttgg atatttttga
    27601 tacattttat caatccagct ggtacttaac caatgtgccg aagtataata aggcatatta
    27661 ccagcagtgt aaaccgttag aataactgga ataggttcat tttttccagt cttaggattt
    27721 acatatttcg ctgcttcttc taacatatgc tcataaaaat ctctagcagc tccatctctt
    27781 ttaggaagat catctggatg taactgtata acagtatatg gttttaagtc atctggtata
    27841 ttctcccact tacctgttaa tgtatctcct ccccaaaaag tatatttccc ttggttaaac
    27901 tcttctccgt tagcatataa aggcatcata agtaacggat gttcattatt aatttcacga
    27961 cgtttatcaa cttctgacca atcattagga ttcttaaatt taccatttgc cataaacaag
    28021 gctccaccta aaaccaattc gtcagcccat gtatgtttcc ctgaatatga tttaagttct
    28081 attgtctgtg tatctttggg aatttcaaca tcaaccagaa atgccgatgt atcatactta
    28141 attccttttg gatattttga atctgatgag taaatgactt taccatccgc aacaacttca
    28201 aatttttgaa tatctgcgta atctgaattt aaatgatttg cactcctatc aattccgaca
    28261 taggaaataa atcttgtaac attagcaccc gaaacatcat attttatact agacgggtta
    28321 cttgctacag ttcccaatcc cttattaaat tcgacttcct taccgtcaat tagtaatttt
    28381 attttattat tagacccgtt attaccaggt gtaaacggtt tatctttctg aactgttttt
    28441 gatttatcgt catcaccatg agtagcatca acccattcta aatcagaaag atatactcct
    28501 ccattaattc ctttattaag aagatctgga gttatagttt gagtactaac agttttaata
    28561 agtttagcat cagcaaatac aacctcatca ccccatgtat gttctcctgc gaatgatttt
    28621 aaggatattt ttttagcgtt ttgtggaatt gtcacactaa taaattgtgc ctgggtatta
    28681 tatctgaatc cttctggatt agttacacta ctaaaataga caacctttcc atcaatttct
    28741 atttctattc tatccactac tgcatggtct gatctagaac tattagcact ttgatcaatt
    28801 cctatataag tttcaaattt ttcaaaccct tgaccgctga tatcatagct gattactgat
    28861 ggagaatctg cgactgttcc tattccttta tcaaaatatt taactttatc atcagaagtg
    28921 agcaatgaaa tcttagtatg ctctccttta tttcctgttg taaatggagc atctttttgt
    28981 actgtttttg ttttatctat atcaccatga gttgcagaag accaatccat atctgaaaga
    29041 tatgtcatct ctttatcaat ttctttatct ttagtaataa gtttttctaa gttattccct
    29101 gttactctag cagtttcagt actagctctg ttattagaat tactttgaac tacatctgca
    29161 taaaccaaat tacttcctaa aaaacttgtt aaaacagccg tagctattaa aatctgtttt
    29221 gctatttttt tactttccat ttttatttcc tcaatattta catacaactt cttcaataac
    29281 aaataatttt gtaagatatt ctggaacaca aagtgctaaa ttacttaaac tgacttttat
    29341 tccagcattc attaattcat ctccataaaa gaactcatca aatctagaga cggaatagca
    29401 tttatcttta tctaaaccac taaaataaac tcttgtataa cctggattca acttactatt
    29461 caattgagca tacaataaaa tcgattggca tttatctttt gaaacaactt gccaactagc
    29521 agaatcacaa ctaaaaggac tgtttatcct atagaaatcc ccatattgaa acaatgaacg
    29581 atatttttta taaaactgaa tttgttctct cacttgttct ttttcttcta ccgacaatct
    29641 cgtaacatct aattcataac cgaaagaact aaaataagct acatttgtcc tagtgtccaa
    29701 actcgtttgt cttccacttt gttcatttgg agaaatagaa acatgggctg tcatcattga
    29761 tggagaatat ccataagaag ttccatgctg aattttcaat ctttctatcg ggtccgtatc
    29821 atcactcgtc cacgcttgcg gtgcataata cataagtcct aaatcaaaac gtccaccacc
    29881 tgaagaacaa gattcaaata gaactgaagg gaacttagtt attaaacgat ctaataactg
    29941 ataaagacct aagatatatc gatgaccaaa ttccatctgc tgttcagaat caagttcaat
    30001 actataaata tctgttatat gacgattcat atcccatttg atataatcca gatttgtttt
    30061 gattatgatt ttagaaatag aatcaaaaag gtaatcaaca acttcctgtc tcgtcaaatc
    30121 caacacatat tgatttcttc ctactgactt ctctctgtct aacaaatgga tagtccaatc
    30181 ggcatgattc ttgtacaaat cactatcaat agaaatcatt tcaggctcaa accacaaacc
    30241 aaattgtaaa cctattttgt gaatttcatc tgcaagaaat ccaatacctt caggaaggcg
    30301 acttctatct gttacccaat cacccagaga acttttgtca tctttcctat gaccaaacca
    30361 accatcatcc agtacaaata attctatccc taaatcttta gcagcctttg ctaaatctaa
    30421 aattttttct gtacccaagt caaaataaaa tgtttcccaa ctattgagta ttataggacg
    30481 actagcttga gaaaaattag gattaactaa atgatgtcga taaaaatctg atagttgttg
    30541 gctcatctga ttcatccctt gatcagaata gatcacaatt gcttctggtg tcacaaaact
    30601 ttcggcagga tttaataaaa aacgaaattc tactggattt attcctaccg ttactctagc
    30661 ggtgtcaaaa tggtcaactt caatcatatt ttgaaaattt ccagaataga taaagttaaa
    30721 accataaacc tcaccactat gctctgtagt ttccctcctt gatagcataa agaaaggatt
    30781 ttgctgagga cttgatgagt atctcaagct atctattgct tgaatacctg gtctaagcga
    30841 agttctatat aactgacgtt ccctcagcca tgctccagaa aattgaataa agtcatattg
    30901 actatcaggt agatctagct gtaagctcat tgctttcaaa agagtcaact tattattgct
    30961 attatttaaa aattccgtat ttctaactaa gacattgtat tccggaaaaa ttgtataatt
    31021 tagtctaaca gttaaatcta atatggaatc tgttaaaata atcgttaatg tttccgcaca
    31081 ctgaccaata tcatcaaatg ttgaaggtag agagttaaga cgatttttcc ctttttcaag
    31141 ttcaaaatct tgatatttta gtgtcgttat cctactacca ttcattcctt gaacttgaaa
    31201 ggctggatgc cgaaaatctc ctttcccata tgaagaatat tcattttgta tcatagactg
    31261 ttgaatatcc tcaaattctt cagaaaaaga tacttcaaat cctctttttt cataggccat
    31321 caattggtta taattttcct tgacatgaat ccttttccca aaatgtagat gttgaagttc
    31381 cccattttct aaaattctaa aaatataact actatctttc attgccaaat gaaaaacggt
    31441 ctcgtcctta ttaatataaa tcgtcatctc tacctcacaa ttttttcttt acttcttcag
    31501 tatcacgata gagtacggtg ccaattctat ctcatcttct tgaatagtaa aatctccaat
    31561 tactctggta aaattctcta tcgaaagatt ctctattttt attttttgtt taatctgagc
    31621 aggatttctc agagagagaa tttcagtaga agtcttctga ttgtgacact tataaccata
    31681 aatttcaaat ttttcaggac ttcctcctat ttttttacta tattttagtg tttgataatt
    31741 ctcctctatc catttgacgg cacgagcatt ggctttccag cgttcttcat caaacatatc
    31801 aaaagaatag tgaaactccc aaaaagcatt ccctcttgtt gaaataaata atagataagc
    31861 ttcaaaatct ggtattgatg caaacatttg atgatccatg taccaggtat ttgcactgac
    31921 agcatagatt ggttcatgat tataaagcga ccacataggt aactgtatct cacgtttttc
    31981 caaaaattct tgatactgaa tatctcggta agttatcata cgattgatat cattacctgc
    32041 attctctgta aagcctacat cttgagatat ttgaatccat aaactattga cccactgtaa
    32101 aaaccatgga ctaggattta cataagaagt caagtttaac caacaatctt ttcctcctct
    32161 ctcctttctt agatctatca acagttgaat taagaactca taaaccactg tcatggtata
    32221 cataccgtgc ggtccactct tatcaggttt gtcaggttga agtaaccagc catcaatttt
    32281 ccaatagctg atatcgaatt ctttttgata ttccaacatc cttttcttca tttgattgag
    32341 atagttaaaa tcagccacgt ttacatcatt tgaaatcaaa tttttagatc caatatttaa
    32401 ctctggatgt gcttctaacc aatcactcat aatgatttct gtcccattat aaccacctcg
    32461 gggaccaatc cacaatccta ggcttgaacc aaatccattt acaagatgtt taatatttct
    32521 caaaccattt gggaatttat gattgaattc ccaaactgat tgatagtttg tccaaccatc
    32581 atcaacaaca taagcatcta aatgaactcc atgattttca aatccatctc gaatctcaga
    32641 aaaacttttt aaaatacctt cctctgtaat atcggtcata tggtcatacc aggaattata
    32701 ctgtttacgg aaataactag gttgagctat tccttcaaca tattcaaaaa atgcctcttg
    32761 aatctcttct tttttataag aacatgctcc cccaataata caagaccaca aactcttttc
    32821 ttgatttaca acagttccca agtaatatct tgatacatag tgtctaccat ctaccttgtt
    32881 ttcagacatt ggaaattcca ttcctaagaa taaagaattc gcataaacag gttgtcccaa
    32941 ttctacataa taaccggaaa aattcgccat ttccttaata caatcttgtc ttttcggata
    33001 gaaaatattg tcttctgttt caaactcaaa actctctacc tctacagatt taataacctc
    33061 agtagatgat tctaaaacta attgcttcga aagaacatca tccctcgatt caaagattaa
    33121 acgtaaacga atggaaggac aaacaaaaga aacctgaatt cgcgtatctc cctcttccct
    33181 aacgattgct gaaaaatgat ttgaagaaag tcttatacca ttctccaatg taatggtaaa
    33241 ttcttctttc gaaacatttt ttatcattac tcctgcccgt ttgtttacga tatcagtagt
    33301 ataaaatcga ccatctcccc aaaagaaatt acgtcttaca tttttatttt caattttcat
    33361 ataaactact ctctcaactc aattttgatt acgctatcaa tcaagtctgg taatggatag
    33421 gtaaaatgtg gaacttctcc aaactgtgca aaacaaattc ctttgtaggc attggtcgtc
    33481 cagctttctg aaattttcac ctcacttcca tcatgaagaa agctcattct ttttacgttt
    33541 tctttactaa taccaagaag agctaaagga cctataggtt gttcaaatac atgagcataa
    33601 accgtatttc cattacgagt atagtatccc caatctggtt ttggtaaatt ttcatctcca
    33661 ctacatccat aaattgcctc accatttcga gacatccatc gatgaaaatt atctaatatt
    33721 ttcttacttg aatcattaat tcttcccaaa gcatctgggc ctacattaag aatcatattt
    33781 ccgtttttac taacacattc cactaacttt cttatcaaag tttgactaga cttatataga
    33841 taatctgtag gattataagc ccaattgtta ttcatagtta gacaaagttc ccaaggaaca
    33901 ggttcacctt taaagtttcg aataccttcg tgaggaacaa tttgctctgg actaacaaaa
    33961 tctccagcgt aagaggtaat ttcatctgta acgatacttc cgaatccttc acctgatgtt
    34021 tctaatctat tatctacgat aacgtttggc tgataatgac gaaccatgtc tattaatttt
    34081 gaagttcccc atttttcacc caccatgtct tcataagaat aatcaaacca taatatgtca
    34141 atctgaccat aaccagttac aatttcttta acttgattgt gtaaatactc caaataactg
    34201 tcaaagttaa ttttctcatc tcgataggct tcatttcctc tcattggatg atttagatca
    34261 gcatatttag ggaaatcagg atggtgccag tcaatcaaac taaaatataa gcctactttt
    34321 aaaccttcag ctcgaacagc atctacaaat tctttaacta aatctctacc tgcaggtgta
    34381 tttgttgctt tataatctgt aaattttgaa tcaaacaaac aaaacccatc atgatgttta
    34441 gccgttaaaa ccatatactt catccctgca gcttttgctt gtttagccca ttctctaggg
    34501 ttatattcct taggatcaaa agctcgaaaa tatggttcat aatcttcaat agataatttc
    34561 tgatgactac gaatccattc cccttttcct ggaatagaat ataaccccca atgaatgaac
    34621 atcccaaatc ttgcttctct aaaccactga gttctactat tgatagtttc aagtgtatgt
    34681 ttcatccttt tactgctcct cctcctaaag cttcaatgaa gtatttactt gtaaatgcga
    34741 atagaatgat tggtggtaat acgattacaa caatagctga aaatagacca ttataattag
    34801 tattaaaact tgtagtaaat tgacctaata atgctggtac tgttaattca tttttatcaa
    34861 tcaccaacat agatgcccaa taatattcat tccagttatt aataaaggca agtactgctg
    34921 ctgttaaaat tcctggtcta gcaattggca acatcacttt ccaaaaagtt tgagcatatg
    34981 tcgcaccatc aatctctgct gcctcctcca attcttttgg aataatttca aaatagttct
    35041 ttataataaa gaaactcata gcaattccac tacaagagta gaccagaatt agagcaactg
    35101 gggtattata aatccccaac tcatttacta atctataaac tggaaaagtc atagctgttg
    35161 ctggaataaa tagagtagaa tacagcatag aagtcaccaa cttcttccct ctaaattcca
    35221 tcctagcaat aacatatgaa gacatggaga tcataacaat gctaataata actgatatag
    35281 acactacttt aaagctattc caaaagtaag tgaaaacatg aagttttgta aaaacattta
    35341 tataaccttc aagagtaaag tcagtcggta aactaatacc tggatttttc ataggatctg
    35401 cttttaatga tgataaaaaa atccacgcaa tagggaaaat agaaattaaa atggtaagga
    35461 gcatataacc atatataaga actttgcctt ttttactaat accatttata tgtgtattca
    35521 taatcatttc ccacctttca gtctttttct ttctctgaat aaaatattaa tacaaacgat
    35581 aatcaacatt ccacctaaaa tttgtataac tccaattgta tttgcacgag catactgagt
    35641 tcgtgagcta gccactgcta attctcgaat aacaaaactg atactttttg ttcctgcagc
    35701 accatttgtc aaaaagaata cttcgttata gagcaaaaac cctgaagttg ctgccataat
    35761 tgaaacagtt ttaattgtcc ccttaatcat cggaatcgta atataccact ctttcctcca
    35821 accagtaata ttgtctaaaa tagcagcttc ttgaacttct tctggaatag caaaaatttg
    35881 cccaagaatc ataatggttg atgctcctgc aaagaagata taagcgccag tcatagctac
    35941 tatatttaat cctggagtca acagtactga atctttaaac gatggattaa atagatgaat
    36001 aatttggtta acaacaccat agtttggatt atagatttga agaaaaatca agcccatagc
    36061 agcactagaa atgatacttg gtacaatata aagattccta gatatcttcc agccttttaa
    36121 tttcttagat aatgtaattg caacagatac agctaaagga acttgaataa taacaccaat
    36181 tacagcccaa cgaacagaat taattagagc ttctgtaaaa aatgggtatt ctttaaaaac
    36241 atacctataa ttttcaaata atttatcaaa tccaagaaac tcaggggtgt ttaaatttgt
    36301 ataatcccat ttagcaaatg aagttaaaaa gattgttact acagaataaa aataaaatag
    36361 aaagaaacaa atcaacgttg gcaaaagaaa taaaaagata aatcgtcttt tttgattctt
    36421 cctattttgt aggctcataa ttttttgtgc cataaacaaa ctcctttttt caactatata
    36481 agtaaaatca agctcagttg gcagtgccaa ctgagcttcg ttttcactcc ctaaaacaat
    36541 taaccaatca atgatttcaa cacatcttga gtagatttta ctttttgatc aacatctaca
    36601 ttttcagcag cactttctgt caatgcattg ataatagctg tctgtacatc gccaccccaa
    36661 acatcactta cagttggaac aactgctttt gcattcttaa cttgagtgat tgcttgtcct
    36721 aaaattttag tagtagcttc tgaactcttt tctgaaagtt cttttacatt tacattttca
    36781 ttagaaggat ttgctcctac tttttcaaaa attacttttt gtacatcatc gctagtcata
    36841 tatttcaaaa actcaagagc tagtttttgt ttagcttcag acatcttact tgagatagtg
    36901 ataccacctc cagaagaact aattgctaca cctgcaggat agattccagg tgcaagactt
    36961 gggttcttag acatctcacc agaagcccac acaccattaa agaatactgc agatttacct
    37021 tcttggaaat ctttggaata agcattggca tcgccaccag cattcttaga accattagct
    37081 tgaatttctt tcataaccat tttcaaagca tctgcaaatt ctttagattc aattccttct
    37141 tttgttaatg gtttatctaa taatttacgt ccattttctg tggttcctaa tactgtatta
    37201 aacaagcgaa tagatggttc tccagcacca aatgcataca ctccatcttg ttttctaatg
    37261 cttgccattg cctgagtgaa atcatcccaa gtattccact gatcaggtgt cttagcaccc
    37321 gcttttgcaa atatgtcctt gttgtaccac aaacccattg taaataactg ttcgtgaact
    37381 gtataaattt ttccatcttt ttggttttgc ttatagttca aaccaacatt tttttcaagc
    37441 ttatttgaat cgatatatgg tttcaaatct aaaacaagtt tttgttcaat cgcagctaat
    37501 gatactgcat tacctgctag gtcaatcaca tcaggaaatt caccactagc aaccttgtta
    37561 ttcattatat ccttcaaatc accatttaca ggagttggtt taaattctac accactatct
    37621 ttatgttctt ctgcaaattt atcatataaa tctcgcatag tcttcgctac aggccattca
    37681 tcttcactat gatgataccc atgataaaat tctaatactt ctttaccaga agaacttcca
    37741 ccgcttgcat ccttagatgt tccacaagct gctaaaccaa aggtagctac taacccgata
    37801 acagcgcatt tcaaaagact ttttttgttc atgacaaaac ctccgttttt ttatttttgt
    37861 ttatgatagc tgaggataat aatattgtgt ctcagcaata tttgtcataa tatcctttaa
    37921 ctctgtatcc tcgtttattt taccttgact caatagctgc acaacaatat tccccacagc
    37981 tgtaccctca gtcaaacctg taagaacctc tttaccagtt ctatcagcaa tcatttggtt
    38041 aaagtaactg gctctagcac ctcctccaat cacatatatc ctcttataaa ccttatgagt
    38101 tagttcttct agtaactcta tcgctttcct atacgtttca gctaggcttt cataaacaat
    38161 cttaaatagt tgtccatctg tccactctct agtttcatga tgataagcta gatattctgt
    38221 caaagtcttg tgcatatcag attctgttgc aaattcagtt gattcagtat caatcagagg
    38281 aagattttct ttttctttct ccaccattgt cctaatatca tcaaaagaat aggctttccc
    38341 tcttcgttca aatgaacgtc ttagttcctc tatgatccac aaccctgtac aattcttcag
    38401 aaatgtaatc actccatctt taccgacttc atttgtaaat ccataactga aggattcggt
    38461 agtaagaagc ggtgaagtaa gttccactcc aaccaaagac caagtacctg atgaaataaa
    38521 taaactacct tctgtcttag gtactgagac aatcgcgctt gctgtatcat gactacaaac
    38581 attcacaaca ggaatatcgc ctaaaccata ctcctttttt atccttccaa gaacatttcc
    38641 ctctgaaaca atctcaggaa gtaaagatga atccaattca aatagtttta agatattctg
    38701 attccagttt tgactcctag gatcaaataa ttgagttgtt gaagcaatgc ttttttctgt
    38761 agcaaactta cctgtcaaga gataattaaa caaatctggc attaaaagaa tcttattggt
    38821 cttatagaaa gagtcaggag attcttgacg tgccttaaag agttgaaaca aggtatttat
    38881 ctccataatc tgatttcctg tctctgaata cagtttttct aattcagtca tttcagatat
    38941 ttcctttaac aatccctttg ttctttcatc acgataatga acaggttgtg ataacagctt
    39001 accttcatta tcaatcagtc caaaatcaac tccccatgtg tcaataccga tagataaaat
    39061 cttgtaacta gtattagcca agcggatact ttcaagaatt ttagctagta gaaagtcaac
    39121 atcccaagat aaatgccctt ttactctaat aggtagatta gaaaagcgat ttatttcttc
    39181 cattactagt ttattttcag aaaggtaacc aacgattgct cttccagaag tcgcacctaa
    39241 atctattgct aaaaccgctt ccatttttct tctctcttcg tccttgatta actttattat
    39301 aatccccacg ttttattttt tcaatatgtg aacgcttact tgaataatgt tcaaatgtta
    39361 acccaatatt tataaaaata attcaaattc ctccaatttg tgctataata tagttgaaat
    39421 gataaattag gaggagctta tgataaaaga tgaacgtgta cttgaattga ttgaaatcat
    39481 caaaaagaaa aaaagaattg ccgtaaaaga gctggcagaa atcactttct ccagcacaag
    39541 taccttacgt cgtgatttaa ttttcttaga aaatcaaggt cttatcaaaa gaaagcacgg
    39601 atacgtgacc ctgtcctcta tgaacacaat tgaactttct catcaaatac gggaaggaga
    39661 aagtactagg caaaaaagac taatcgctag tctcgctaaa gactttattc ggtctggtat
    39721 gtgtatctat ctggattcca gtactactgt ctacgaactc tgtccctatc tttctgaact
    39781 tgataatttg attattttta caaatggttt acatactgca caaaccctat ctgaaactgt
    39841 taaagatagc tccaaaatct ttatcacatc tggcgaggtc aaacatcaat cctgttccgt
    39901 ggtcaactat gataaggaaa attctttatt agatcatttt aatatcgatt tagcattttg
    39961 ttcagcaaga ggtattgatg accaatatgt ttatgaagct tctctcagcc aagctatttc
    40021 aaaaaagaat attattgaca aagcccatga aaccatctta ctgattgata gttctaaatt
    40081 ttacaagact ggatttttta aaattaatcc cctctccaaa tacacaacct ttatttctga
    40141 caccttgcca gaccaaaaat tattagacgc agtcgaatta tttgatggag aatgggtttc
    40201 tgatattcaa tgaaaatcaa ataggaagct agtctcagct tgctcaaagt acagatttga
    40261 aaactgatca agtcaagtcc cccatggaca cggcaagacg aagctgacat gatgtgaaga
    40321 gattttcgaa gagtattcgt tctgtctgct gactgattta caaatattca ccacatgaca
    40381 ttattttctt agcaattcta aaaacagagg ctggacaaaa actcaatttc aggagaaaat
    40441 ggagtaaacc ttcccacaat aaaacgcata gtatcaagtt tttgcacacc tgatattatg
    40501 cgtttttctg attttaaaga ctttgtcgcc tagaccctat atttcaagaa ttaagtgacc
    40561 ttgatgatct aaatgtattg tggtatacta tttttgtcat cggttaccga ttacgttcct
    40621 atgggttcgt gagaggaggt gaaactaatg agccttatca tagagctcgc tcttactatt
    40681 atagcggatg ttatagctgg aattatcttg tatttcgtct gcagatggct agatagtaag
    40741 gagtagaccg actgactagc ctataaacac ccgttaaatc gctaagatac gtcaaaaaag
    40801 cccttaacta cggccatagt taggggcttt ggtgttctaa tgaaccttat caattaacta
    40861 cattttagca tataatctac aatagttcaa gtattaagtg aggttgatga tctaaattta
    40921 gtgtggtata ctatttttgt catcggttac cgattacgtt cctatgggtt cgtgagagga
    40981 ggtgaaacta atgagcctta taccagagct tgctcttact attatagcag atgttatagc
    41041 tggaattatc ttgtatttcg tctgcaaatg gctagatggt aagaagtaga ccgactgact
    41101 agcctataaa cacccattaa atcgctaaga tacgtcaaaa aagcccttaa ctatggcact
    41161 agttaggggc tttggtgttc taatgaacct tatacactaa ctacattcta gcatataagt
    41221 ccagatattt caagagtttt atttattttt tcaggttccc ttagttctga aaggtctata
    41281 atgaagttag ccatctagta tcaaaaaacc gactagctct tatgaactag tcgatttctc
    41341 atcaatgcgc caacatttct tgggcgattt cttggccaga taggttatct gggtagtagg
    41401 ttggccaatt atccatttct tcaaatagag cttcttggct tgtacctcca aaaaagatat
    41461 ggaaatgttc tgccttaact ggggcaacat tgtggtcact aaactgaaca tatttgaatt
    41521 gtccagcgtc agcatctgtg gcttcaaaga ggaaacgcac gccacgattg cctttcttgt
    41581 aagtcaaaat tttcttaccg acatacttgt aagtgtattt cttgctttgt ccaccttgaa
    41641 caaattccat agtattatca gtaatgttaa tcttagtcac atctgtctga tagccttttg
    41701 tatagtaagc cttgtactca gcctgggtca tcttaccagt caacttagcc ttgtagtcaa
    41761 agacttggtc aaacgtgccg tcttcaagga aagggtaaac tgattgccag ttacctgcat
    41821 agtcactcaa ggtgcggtcc ttgacagctg catcctcgaa gtaaccattt tggactgtct
    41881 tggtatcctc tgccttttca ggttcgattg ctgggccttc ttggtctgtt gtttgtttca
    41941 aagccttgag gtttttctcc atcacggaaa tgtagttttc tccagccttg gtgtcctctt
    42001 ctgtcagact ttctaaagga ttgaggacat cagttttgac acctgcttct tttgaaagtg
    42061 tgttagcaag ggcttgtgag gcattttctt caaaatagat ataggcgatt ttatttttct
    42121 tgacatactc tgtcaattct gccaagcgag cagctgatgg ctctgcatct ggagaaagtc
    42181 ctgagattgc gacttgtttg agtccatagt ccaaggcaag atagttaaag gctgcgtgtt
    42241 gagtcacaaa gctcttttgt tttgcttgag acaagccttc tgcgtaagcc ttatccaagg
    42301 cttgcaattt ttcgatatag gcagctgcat tcttctcaaa ggtctctttt ttatcaggat
    42361 aatctgctga caagctgtcg cggatgtgct ctactagttt aatggcacga actggtgata
    42421 accaaacatg ggggtcaaac tcatggtgat gaccttcttc tccatggtca tggtctccct
    42481 cttcttcctc gccacctggc aagagcaaca tatcgcctgt cgccttgatg gttttcactt
    42541 ttttcttatc caaggtatct agcaatttag gtacccatgt ttccatgttt tcattttcat
    42601 aaacgaaggt atctgcatct tggattttgg caactgcctt ggcagatggt tcgtattcat
    42661 gaggttctgt cccagcaccg attaggagtt ctacattagc cgtatctcct gcgacttgct
    42721 tggtaaattc atagacaggg taaaaggttg tcacgatatt gagtttacca tctgcctgtt
    42781 tttgattgga acaagccact aaaaacaagg cacatagact ggctagtaat aagctaattt
    42841 ttttcacgtt cgtctcctat ttgataaaac gtcttactaa actgattagt ataaagacag
    42901 ttacaaaaat aatggtaata cttgcacttg caggtgtttc tgcatagtag gaaatgtaaa
    42961 gtcctgctac cattcccaaa aagccaatcg cactggcaag cagcataacc gatttaaagt
    43021 ttttccccag acgcagggca atactagctg gcaagaccat aatggtcgat accagaagag
    43081 ctcctgctgc aggaatcata agggcaatag ccacccctgt caccatgtta aaaagaatgg
    43141 acatggtacg aactggcaag ccatccacaa aggccgtatc ttcgtcaaaa gttaagatat
    43201 acataggacg aagaaagaga aaggtcaaaa tcaaaacaac cgccgcaatg acaaagaggg
    43261 aaatgacctg ttcttcactg atagtcacga tcgaaccaaa gagatattgg tccaaactca
    43321 ttgaactcga gcttttaccc ttgctcatga caatcagaga aacagccaga cctgttgaca
    43381 tgaggatagc tgtcccgatt tccataaagc tcttgtaaac cgtacggaga tactccagaa
    43441 agaccgccgc aatcaagaca atggcaatag tagaaacagt tggagaaatc cccaaaacca
    43501 gaccaaaggc tacacctgaa agtgagacgt ggctaagggt atcactcatc aaactctgac
    43561 gacgcaagat gaggaaggtt cccaataccg gtgagaaaag actcatagca ataaccgcca
    43621 aaaaggcgcg ctgtataaag tcgtaagata ataaactaag catggcccac ctcctggcca
    43681 ttctcatgaa cattgaaaca acgccatggc gagtcttggt tacggactag atgaatattg
    43741 cgatccgcat aatccttaac ttcttcaggg tcatgggtaa tcatcaaaac agccttgcca
    43801 tgatgatggg cgctgtggtg catgagttcg taaaattcat ttttacttcc tgcatccatc
    43861 cccgttgtcg gctcgtctag gataaacaca tcagggtcag aagcaaacat acgcgcaatt
    43921 accgctcgct gcttttgtcc cccagataga gaccccaagc gtttgtctcg atgttcccac
    43981 atgccaactg agtccagact agccttgata tgctcctcat catgagcatt caaacgacgg
    44041 aaccagcctt ttctcggata gcgacccgac ttgacaaatt catagaccgt acttggaaaa
    44101 ccagcattaa aactggcaat ttgttgagga agataggcta ttctcaattt cttaccttgc
    44161 gtatttgtct ttgaaatagc cacctttcca atgcgtggtt gcagaattcc aagactagcc
    44221 ttgatgagcg tcgtcttagc cgctccattt tccccagtca aggtaacaaa ttccccacta
    44281 tcaacacaat aattgatatg ttcaagaaca ggctccttat cataatagaa ggacaaatcc
    44341 tctaccgtaa tatatctcat tatttgattt ctcctactaa agcagtcaaa aaccgctgaa
    44401 tcactttttg ttcatttgga gtaaactgag tcgccacttg ttcataggtt aaaagtgtat
    44461 gctcatggtg atggtggtgc tcctcagcga ttggacgagc caagtcagtc aactgataaa
    44521 aaatcacacg cgcatcttta gaatctttag atgtttccaa catcccttcc ttgaccaaag
    44581 acttaatggc cttggtaact gccgcctgac tgacattgag acgacgggcc aattctgaat
    44641 ttgttaaaga ttcctctgac aagagcataa ggatatgctc ctgagtattg gtcagggcca
    44701 cctcgctagt gcaatgacct attaggattt catgctgatt ttccgcctgc aaaatcacct
    44761 cattcaaaaa agcattgata tcctttgcta gctgtctcat atctgactcc tttcctttta
    44821 gacttctctt ttttaagaga aaaatactat tctttgacat tttgtttacc agttaattat
    44881 atcacaagca aaaaaagagt caagaaaaaa cgtgaaaact agtttcattc ttgaactctt
    44941 ctatattata ttatctattg aaattctttg acatctccat cataagtcgc ccaatctttg
    45001 ctgaaaaagc gctcattcag atggtaagtc ggagctggtg tgggattgga taggaaagga
    45061 tcaactgcct tgtcaaaagc caaccaaccc aaccaaccaa ggtgaatggt gtccttcata
    45121 aagaaaggct ccccgccgtc cttagaaaaa tctgctatat tggtaaaacc ttgactttct
    45181 aactggtagc gaatcttctg caccgtttgt tggtacatat cctctcgtag accagcatag
    45241 tccatccatt ttttattaac aggtggaatg ataaaaatcg ggtttacctt agatttagaa
    45301 aactgtgtta aaaccaactg caagtcatta tactctggcg acttgagata ggtaaagctt
    45361 ttctgagaat cctttaattt cttcaaatcc ttcttgatct gctcattata gaaataattt
    45421 tccattccca tctcattatt ggaagtattt ttttcagcat ctgctttgac aacatcttct
    45481 attgcttgat aagaaaactg gtctggcaag atttttaaat acttagctac atgcttatcg
    45541 tagttaacat agcctctaac cgaaaactga ccaaaaaagg aggcttggcg ttcattaaaa
    45601 cgagccaata attcaatcat ttcattgtct gctgtcgaca attcttcttt acttgccaac
    45661 ttctgaacca ggtccttcat agctacgttt gggaactgtt gcagtaagcg agtcgctgca
    45721 tattgactag cctgatcccc agattgatgt ttcagaaaac tagtcaactg gtctccatta
    45781 aaatactgct ggaaggctgc tggatcatag ccatttttac tgaaccactg aggtgagata
    45841 acatacacaa cttgtttatt ctccagctgt ggtaacatct gttgcattcc aaaatattgg
    45901 ttaagcgatg cagctccccc ctgtcctaaa agataaggac ggtaggaacg attgtatttc
    45961 tcagctaata ccgcaggatg agcaccgtca aaacgaagcc attcactaga gccaaagaag
    46021 ggaacaaaac gcacatttgg atcagatagt gctctgactt tttgacttcg ctccttaaaa
    46081 ctatcgatag tagtagccac tgctgaacgc ttttcagctc ctagattatg atgcatctca
    46141 gtaggataaa agaaaatgag cagaaaaacc aacaaaccag cgatcaagac cggtccgaag
    46201 atcatccata agcgtttaag cattttgtag ctccacaata ccagctatga ttttattagc
    46261 tgtattccag tcgtcacgac caaactctgt tacagggaca cgaatgtcaa aacggttctc
    46321 aatctccaca atcaactcaa ccgttcccat actatccaag acacctgcat caaaaagatc
    46381 ttcatccatc atgtcagaaa catcttccat aaacaactca tcaataattt caataacttc
    46441 tgatttgata tccatatttt atttcctttt attttttaaa ccatagatta ttcaagaatc
    46501 cagaaaagat taagaatgac aacatgacaa catggaaagt gacaaccatg ccaagcaact
    46561 gaatccagcg attctcaggt agggcagcct tccctgcttt tttccgttcc ttattgagcg
    46621 tttttttctt gcgaacccag gcatcattga tgaccaagcc tagtccatga aagagtccat
    46681 aggcgatata gtaccaggtc acaccatgcc aaaatcccat aatcagcata tttacaatgt
    46741 aggccatgct tgaggttaca ttacgatttt taaagacttt ctttctggtt aacaccatca
    46801 ccattcgcat aaagacaaag tcacggaacc agaaggacag actcatatgc cagcgattcc
    46861 aaaactcctt taaatccctt gataaaaagg gcttgttaaa gttgataggg ctacggattc
    46921 ccatcaagtt tgagatggcc aaagcaaaca tagaataacc tgcaaagtca aagaagagct
    46981 ccagaccaaa agtatacata actgccaagg catagagatt aaagaagcca cctgactgca
    47041 aggctaaatt cttcagagga ggtagtaagg tctctcctaa aacatgagct aggataaact
    47101 tatacaaaaa gccccacatg atatagcgga cagattcatc cagcatatcc atcaactcat
    47161 ctcgctcagg aatagcctga taattttcat taaatcgctt aaagcgatcg attggaccac
    47221 tcgagaaagt tggcatgaag agaaggaaac ggaggaattc ccagagggta aaatccttaa
    47281 tcactccatc tctcagctcg atgataatcc caaccgaacg aaaggtcagg taagaaattc
    47341 ccaagaaccc aagcaaagac tgcgttccat tgatagctgg ttgcaccttg acaaagataa
    47401 tcggaagtag ggacagaaaa ctaactaagt agaagaccca cttgccatcc ttgctttttc
    47461 gataatgctt gtagaaaagc aggagcaata tttcccagca aaggtaaata cccaaggcag
    47521 ctagttgatt ggtctttcca cccaccaaca tggtgacaat aaagaagaga cttaccaaca
    47581 cttcatacca ggcaaagcgt ttcttgaaaa agaggcctat aaagatgggc aaggttgcag
    47641 caatcacata aacaaaatac tgaggattgc catatggctc taaatgagga agctgttgaa
    47701 aaaactccat catctcttat tcacctcgtt aatcaatcct ttgatgtcaa tctttccatt
    47761 tggagttagt ggcaaactgt ctcggtaaag gaatttagat ggcatcatgt aggacatcat
    47821 gatgtctgtc aggtcttcct tgatggcctt ggtaatatcg atatctcgct caaactgctc
    47881 acgaacaccg tcttttaaga tgacataagc caatagattt tgtaccttgt ggtccttgtt
    47941 atagcgcggt actgcgacag cagattcgat aaagcgagac ttgttgaggt tttgagagac
    48001 atcttctaac tcaatgcggt aaccgttaaa cttaatctgg aagtccatgc gtccgccgta
    48061 gagaagcaag ccctcatctg tcatggttcc cacatcgcct gtgtgatagg ctggcagacc
    48121 ttcaaactca aagaaggctt ctgctgtttt ttcaggattt ttcatataac cttttgaaac
    48181 agctggccca gaaacaatga tttctccctg ctcaccattt ggcagtttat ttccttcctc
    48241 gtcaatgata aaggttggag aatcagcctt ggtatagccg attggtaggc gtttgagagt
    48301 cgctaacatc tcgtctgtca cggcaactgc tgacagagct actgtcgctt ctgttgggcc
    48361 gtaagcattg atgatacggg catttgggaa acgctcgcgc agtttttgag ctgttttgac
    48421 cgtcaattct tcaccatcaa agtagaaatg cgtgattcca gacattttct cactgttgaa
    48481 gtattcagac aacatggcca tatctgcaaa ggatggtgtt gatgtccaga tagcgattgg
    48541 caatgaaaag atagccgcaa agagttgctt aaagtcctga gtgatgactg aaggaagagt
    48601 gaaaagcgta ccaccaagtg ccaaggtcgg tgcccaatac atgacagaca agtcaaaaga
    48661 ataaggtggc tgtgccagca tttgcggacg actcggtgtc gcaaattcct tatccgtaat
    48721 catccagttt gtaaagctga ggagattatc atgtgaaatc tgcactccct taggcttacc
    48781 agtcgtacca gaagtaaaga taatgtagta attatcatct cccttgactg gatgcgtgat
    48841 ttcatagttg ttcccttggg caaaggcttc ttgaacctga gctagattta tcattggtgt
    48901 agaaacctgc tccaagggaa aggctgaaat ggcaataatc aagcttggct ctgctacttc
    48961 taaaatagct gaaactcgct ccaaggccga atggctatca attggaatgt aggcatgacc
    49021 tgacttagtc agcgctacaa aggttgccaa catttcatat tcttggccac caaaaacaac
    49081 cacaggagac ttctcaggca agcctagttg gtcaatgact gcagccaaac tatccgaatc
    49141 agcctttaaa tcgccataag tgtgttcctg ccccaaaaca ttatagacag gatagctagg
    49201 ctgtgtctga gcaaaatgct caatggtttc aatcatatct gctattggtt tatttgacac
    49261 aatagggatt ctccttcaag ttaaaattca ttatagataa agcttccttg accctgacca
    49321 agatagctaa agaagtaaag cagccctaga aagataagaa aatacaaggc tgtccgacca
    49381 agaaagaggt acaattcttt tctctgtttc atcaagaaaa accattcatt tctgtaattt
    49441 ttcgctaaaa taagagtgat tcttactagc ttatttttct accattgtac cactttatat
    49501 agtatctttt caattgttta ccgtatgttt ccaatagatt tcagcttatt ttaaggatta
    49561 tacagttttt ctatgtatat tttcaaatag agtgatcctg cttcaaaact ccatttcagg
    49621 agacaatgaa gtaaatcttc ccataataaa acacacaata tcaagttttt tcaacacctg
    49681 atactatgcg cttttctgat ttttaaagac tttttaacca ctctctcatt taaaataatc
    49741 tcgtctgata taaattaaaa tagcttctat catcagacaa atggctgata gccaaaaact
    49801 gatgctaata ccaaaactct cagtaatata gctcattagc aaaacaaata ctgaaaatgc
    49861 taatgtagaa atcacttcaa gaacggaata gacattaact aaatgatttt cctctactgt
    49921 ttcctgaaga aatacacttt caggaacttc ttttagttgc gataacatac caactaaagc
    49981 tgaaaataat aaaaacatct gtgcgtttgg aaaatataga atagtcagtg tcactattgc
    50041 catagctaca agaggaaaaa gaatactttc ccatttagca gcaaggaact tttcagatag
    50101 cctaaaagca attaagccac taattataat accaatagag tatgctgtat tagaatatcc
    50161 ccagtaactt tccgttttat ttaataactc cgttacaaaa acaagtataa tggaagaaac
    50221 ccaaatcgta tttgaaaaaa tttccaataa atttgctgat acaaaaagtc ttaatctagg
    50281 atttctagca actaacttcc aacctttgag caaaatttca agattagttt ctgactctaa
    50341 cacctccact tcagcgttag gaagaaataa catcagaaag ctagaaatga tatacaagac
    50401 taaaatgata aacgtggtag gtaacagacc aattgttgca aataagagtc cacctaatcc
    50461 ccaacctatc aattgaacag cttcaccagt cattgataag gctgaattag ccttacccaa
    50521 atcggtcgca tagcgtggca caatagcata ggaaacgggt gctgcaaaac catctagtat
    50581 ggaaattgca acaacaaata gataggtcac caaaggcgct acggattgca ttacggtaaa
    50641 cattcctacc agtatcgcca ataatatagt ctttccaaat tgagataaag ataaaaccct
    50701 atttagcgct aaccttttag taaccaacgg aactaaaaga ctcgcaacaa aagaggatat
    50761 tcctattaag ataggaacta gtgatgtggc aattactgat tttgaaataa tgtatatgtt
    50821 agcaatgatt gttactctaa agaaaatatc tgctaaattt gcaaataatt gagagactaa
    50881 caacttgaca aatgaattag acatgtagcg ctcccccttt agttacaaac tttggaatta
    50941 aatttgattt ttaatatttt agcataaaat agcagaaaac aaaagtgagg ttttttacat
    51001 gaaaaaacaa aaactattgc ctttattatt cctaatttta ggaggaatta tgattatcgt
    51061 tgttggctag tactaagaag ttagttagtt agtagaataa ttacatcttc aatcgaaaaa
    51121 gagactggga aaaagtctta aaaatcagaa aaacgctcaa atcctttggc gctctaagct
    51181 tttctaatga tagattttag atagtcaaaa aaatatctga aaagttccca gatcgtcgtc
    51241 tcagcatcaa aaatacgtga aatttgcata attaaaataa atttcagggg agttaatcga
    51301 gtaggggata atctctagcc cctctcacac caccgtacat gccgttcggc atacggcggt
    51361 tcaactaact tttaacgcat gtcgttcaag gtaataatcc aaacacgaaa ccagtccacg
    51421 tttttcaagg actggttttg atatagcacg tttaagtacc gacttctgag ctactaattg
    51481 ataatggtcg ccccagccag ataccttatc tgctatccat ttaggaactc ctaacttaag
    51541 caatccccat agtcgtctcg atttcttctt ccattgcttc cagataatca ctcgtaggcg
    51601 agtacgcaag cgctcatcta tgctagcgac tatgcgcttc atatttccca gcgagaaata
    51661 gtttatccat cctcgaatag acaaattcag ttgctcaata cgtcttgtta agtctatact
    51721 ccatttcctc tgtgttagtt tcttcaattt aagcttaaat ctccgaacac tatcttgatg
    51781 gggacgactt ttccaaccat ctgatgattt ccagaaccca aaacctaagt atttcagctc
    51841 tcttggtctg gtaatcttag ccttggtcat atttactttc aaacctagtc gcttctcaat
    51901 aaaacgactg gctgaataca tcacacgctt agcggctgcc tcgcttccga ccgtaatcac
    51961 acaatcatct gcgtagcgca caaatcgaag tcccctcttt tctaattcct tgtccaattc
    52021 attaagcatg acattggata agagaggaga taaatttcct ccctgtggcg tgccaactag
    52081 cgttttatga cgctgaccat taatgataac acctgaatga agatacttac gaatcaggga
    52141 ttccgtatct ccgtcttcga taatgttatg taccaaggac atcaatctat cttgaggaac
    52201 cgtatcgaaa aatttctcta ggtctatatc cactatccac tcatagccgt catttaagta
    52261 ctctaagaac ttcatgatgg ctttttcaca tgacctattt ggtctgaagc cataactcgt
    52321 atctgagaaa tggggttcac aaatggggct aataacttgg acaatggcct gttgaatcat
    52381 tctatccata actgttggaa ttcctagttg acggatacct ccgtttggtt tagtaatctc
    52441 aactctaaga actggttgag gcttatattt tctctgtttt atcagttcct tagtcaagcg
    52501 ccagttttgt ctgagataat tatccatctc ttcgatagtc attccatcaa tcccagctga
    52561 gcctttattg gattttactt gattgtaggc ttccagcata ttttcgcgtg ataatatctt
    52621 atctagcaat tttgacatgt gcgtattcct ttcttgtttt ggtgcctgag ggacatctta
    52681 tattggtgaa ccttcatcta gtcattacgt gtcaaagtcc agttctatca gaccactcat
    52741 tttacagaca caagtgaagt aggattactt cgatctgtcg ttttctcttt ctagcgacag
    52801 acctcaaaca gtctctcccc agactgtttg agtattcacc ccttcgctcc acttccatta
    52861 cggaagtttc ctcactacta tgggctcggc tgacttctca tgattcgttg ttactacgct
    52921 ttcagcgctc atgaaacctc acgggataag tcgtacatct ttcctcgttt atacaagata
    52981 tgaggacgtt aaaaggttac agtgaaaata ggaaatctga cgcagagtct ttagactcta
    53041 gaaagattta tctttttcac acaaacctta gtccgaattc aattactctc gtgatttaca
    53101 catataggtt acgactacct ttttggactt tagagtttga agcctcctta tccgctatat
    53161 acgccttgct atcacgttcc tgttcgtaga gccacgattt cgctatctct tcctctgacg
    53221 attcttattt cctgcgtcag acttaaaacg gtctatcccc agaccgtttt aatccgctac
    53281 ctcacgatag tcaggcttgg gaatcgcttt tgggttcacc ggtagcaagt gcccacggag
    53341 gacttgcacc tcagatatac gacatgcccg tcgtactaca aaaaaacgac gatttaaatc
    53401 gtcgttttac taagataaat atgcattttt tttgaaaata cgtctattta ttttttattg
    53461 ttttttattg tagagcagac ccaagcgaat gaacataggt tgtaaaaagc aaatgaaaaa
    53521 actgcgagga taatagcttc tgtgtctaaa taacccattt cgagacctct tttcaacaac
    53581 aacaagcctg tagtttcgat aacgatcaaa cataaaaata tataaaaaat tttcgcatta
    53641 agcatattgt taccgctttc tttttatttg gttttattgt ataataaaag ttgaaaaaaa
    53701 tcaaaaatac aaaggagttt ccgttatgtc taagatgttt aaatgcatgt gtttgctagt
    53761 tatgtctgtt ttctgtatgt ctagcatttc cccgtcgctt gttgtttttg ctgatgatag
    53821 cttatcggca aactctaaag ttgtatctgg tgaggctcag tttgagaatg gctcatcagt
    53881 gcggttcgga gatacgcaag taaatatttt aagcgatgaa gttcttgagg ttgtcaatcc
    53941 agatggaagt gtagacacta ttgaacgacg tgctgacggc gtctatatca atggggcatt
    54001 ttatatggct tatcaaaaga atgaaatcga cctaaatata tcttttagat catacgatcc
    54061 taatgtttga aattacgtca acacaataca tgggaataaa caagctaaca cattcgctaa
    54121 cttcatgact ggtgctggaa tcagttatat gattggtagg ataggtgctt tattgggggg
    54181 tccatggggt gcaataattg gtggagcata ttttggtata caagcttatc agtcttactt
    54241 ggattcacaa agcccatatc catactacat aacaagtact tatatccatg ttgctcaaag
    54301 aaaatggaag tttatcactg aatactatag aaactcaaac tatactgggt atgtaaaaac
    54361 cgtaacaacc tacgtgaatt tctaaaatat aaaccctgcc tagatataaa ggcagggttt
    54421 atattttagt taattgctca agtaatttgt aagcataata cttgcgaaac tctatttgtt
    54481 caagcggttc aaaagctctt gttttttatc ctcaagctca gctctccttt gctcgatttc
    54541 ttcacgtttt tgggcgattt ctgcttgcgc ctcgggagtt tccaaagtgc cttgtgactt
    54601 gtgaaattct actagcatat caacgatttt tgggtctaat tcgttgacat aatcagaatt
    54661 tgactatttc ggcacgttgc ttgcaggtgc ttgctttttg ccgccttttt tgggcgtttt
    54721 ggcttgttgt tcagctttct gactgtagtt gacctcgtgg aattttcgtg cttgatatga
    54781 tgcgttttat tatgagaaga tttacttcat tttttgctga aattgggctc ttgcccaagc
    54841 tctatcaaca taattgtttt ctaatactca atgaaaatca aagagcaaac taggaagcta
    54901 gccgcaagct gtacttgagt acggtaaggc gacgctgacg tggtttgaat ttgattttcg
    54961 aagagtataa actagaaaag tgagaataca agcacggcca aggctgctcc gataacaggg
    55021 cctacaacag gaatccaagc gtaagaccag tctccgtctc ccttgtttgg aattggcaag
    55081 atgctgtgca tgatacgagg tccaaggtca cgagctgggt tcaaggcata acctgttgtc
    55141 ccacctagtg atagaccgat accgacaatc aaagttccca ctgcaaaggt tccgatacct
    55201 gcctgaaaat cgtaaagacc caaagcaaag attgtcaaca ccaaaacaaa agttccaagg
    55261 atttcgctaa tcaagtttga tacagtatcc ttgatggctg gtccagtact gaaggttgcc
    55321 aggatattgc ctgcattttc ttctacctca taatgcggtt tgaattgcaa ccaaaccaaa
    55381 atctgaccca gcatagcccc tgcgaactgg gctaagatat aaggcaaaac ggaagcccaa
    55441 ggcaagtcac cttttaaagc tacaccaact gtcacagctg gatttaaatg agctgggctg
    55501 agttttccag atacaaatac tgcaaccgca actgcaatcc cccaacccat agtaatcaca
    55561 atccaacctg cactgttgct cttggttttt ggaagaacca cacctgcaac aacaccattt
    55621 cctagaagaa tcaggattaa agtccctaaa aattctccaa ataattcact catcattttt
    55681 ctgtctccat taaaaaaggg gcgggcgaca aggattgcta ccctccacct cttttacttt
    55741 ttcttaattt tttaattctg ctaaatcatt attagcaaga gttgctttta catcagcacg
    55801 gtaagttgct ttttcttctt ctgtccagtc atagaatcgt cccatttcat ccaaaactgg
    55861 ctcaacgata ctatccaagc tatcacgcat aaagagcatg tgattggtac gacgaagaag
    55921 gaagtcaact gggctaagag tcaactcatt gcgcattgca tagtgaaggg acaaggtgtc
    55981 tgccaagcta agacctggcg cttgttccaa gctgtgagca agtgcaaaga ccttcggtgc
    56041 atttgaaccg taaaaatttg ctaggtaatg agcttccttg ctatccaaac cacgtgaaac
    56101 tccaagttgc gcaaaggctt cgatttctga atccacattt gctgggttca attctccacc
    56161 tgaaacaggg taagttttag aattgatcaa tttaaagcta cggtcaaatt ctgctttgag
    56221 gatgtcaacc acgcgctcca tagctccttc agccatctta cggtagtctg tgattttacc
    56281 gccagcaaga gtcaaaagac cattgtcatc acggtccaag ctagacccac gagaaactgc
    56341 agatggatcc aaatgtttct cagatgtgct actttcaagc ttgctgacag cagactcaac
    56401 atcttcacgt gttttttctt tggagagata agattcaaca gtcgcaatca agttgtcaaa
    56461 gctttcatca ctgatggtac cgttatttcc accattatag tcagaggcac tgttccctgc
    56521 aatcaatgga cgaagacctg cccagctgct ttcgatatca tcaatggtga tgttggattc
    56581 tggaaagcgg ttgttgacaa tgccaagtag ataatctaca tcttcttgag ttacttttgg
    56641 atgctccaaa tcacctgtgt agtctgtatc agttgtacca aagtaagtct tgttttcacg
    56701 tgggagaaca aagaccatac gaccgtcacc caaacctgtg tcaaagtaaa ctggctgtga
    56761 aaccttgatt ttgcttgaat ccactaccaa gtgaactccc ttagttgggc gcatttgtga
    56821 gaattgcgtt cccttattag acaaattacg tactttatca ctccaaggac ctgttgtatt
    56881 aataaccaga cgggccttga tttcaaatac ttggtctgtc aagagatcac gagctacaac
    56941 acctgtaatc ttgccacttt cgtcaaagag gaagccttct gccttcacgt ggttggcaat
    57001 gagggcaccg tcttggttgg cacgtttgat gttttcaatc acgagacgcg catcgttgtt
    57061 acggaagtca agatacactc cacctcctac caagccttcc ttcttcaagt ttggctggcg
    57121 ttccaagact tgatccttgc tcaaaacctt gttcgcagct gacgtgttgc tcacacctgc
    57181 taaaaggtcg tacaagtcca tggctacttt aagacggaag aggctaaagg ttgctccatc
    57241 ttcatcgtaa actggtaaga gcattggatc tggttttgga atgtgtggag cgatttgttg
    57301 aaccactgca cgttcagaaa ccgtatctga gaccacttct acgtcaaatt gtttgaggta
    57361 acgaagtcct ccgtgaacca attttgttga acgactagat gttccttctg caaagtcttg
    57421 catttcaatc aaaccagtct caagaccgct agctgccgcc tgcaaggcta caccagctcc
    57481 tgtgattcct ccaccgataa tcaagaggtc cagggtgcgt tcctgcattt ttttaattga
    57541 caattcacgt gttttctttg aaaattccat atttacacac tttctaaata aatcgcttta
    57601 ttctgccagt attagtcgtc tacttccgca aagacttgag ttgctttcac agccttcttc
    57661 cagcccttgt agagttgttc cttgcgagat tcattcatag atggctcaaa gagttctcct
    57721 gtctcgttca agagtttcaa ctcgtccaag tctttccagt accctactga caaacctgct
    57781 aggaaggccg ctcctagagc tgttgtttcc aagtttttag cacgtgcaat gtcaatgcct
    57841 aaaatatccg cctggaactg catgaggaag ttgttcatgg ctgcaccacc atccaccttc
    57901 agtacttgaa tggcagtctg agtatccact tgcatagtgt cgatgatgtc acgcacttga
    57961 taagcaatag attgcaaagt cgccttgata aagtcttctt tgcttgttcc acgagtcaaa
    58021 ccaaagacgg aaccacgagc attttggttc cagtatggag cgcctagacc tgtaaaggct
    58081 ggaacgacat aaacttcatc gttgttgtga gaatcacgag cgtatttttc agattctggt
    58141 gaattttcaa ccatgcgaag accgtcacga agccactgaa tagcacttcc tgcgatgaag
    58201 atagaacctt ccaaggcata ataaacctta ccgttgattc cgtaaccaat ggttgtcaag
    58261 aggttgtttt cagacaactg catctcttcc ccagtattca tgatgatgaa agagcctgtt
    58321 ccataagtat tcttaaccat acctggctca aaagccaact gtccaaagag ggctgcttgt
    58381 tggtccccag ccatacctga gattggcacc tctccaccgt agaaatggaa tggagctgtc
    58441 ttgccgtaga tttcggagtt agaacgaact tctggaagta tagccttcgg aatgttaagg
    58501 atttccaaaa tctcatcatc ccatttgagt tctttaatgt tataaagcat ggtacgagct
    58561 gcatttgagt agtcagtcac gtgagccgca ccgtcagtca atttccaaac caaccaagta
    58621 tcgatagtac caaagagcaa ttcccctttt tctgctcgct cttgagcatc ttctacatga
    58681 tccaaaatcc aacgaacctt ggtagcagag aagtaagcat caataatcaa accagtcttt
    58741 tcatggaatt tttccacata accttggctt tttagttgct cagccaaagg tgctgtctgg
    58801 cgtgactgcc aaacgatagc attgtagata ggaagtcctg ttttcttatc ccagacaacc
    58861 gttgtttcac gttggttggt aatcccgatt gcctcgattt gatttggctt gacaccactt
    58921 tcgatgaaag cacccgcaat aactgactga acagagttcc aaatttcatt ggcattgtgc
    58981 tcaacccaac ctgcctgagg gaaaatctgg gtaaactctt tttgactcga gctaaccttt
    59041 tccccttttt tgttgaaaat gatggcacga gaacttgtag ttccctggtc aatggccatg
    59101 atgtattttt cttgtgacat gtactgtcct cctgataaag atcttgaggt gtttcctctt
    59161 tacacttact agtatacaaa ataaagcgct ttcttgttta tcaacttttt ttaattttta
    59221 aaaaaatgtg aggagattgt gtgaatttca caaaaaagac ctagattaac caagcctttt
    59281 aagtgaataa taactgacga atccctgcca aatcttccat atccaagtca ttttttaaat
    59341 aatagactgg tgtctgttcc ttcttatgaa tagggttatt ggtaaccagc aaatcataat
    59401 gccgactagg gtcataagct tcaatggtaa taaaacgatt gtattccaaa taccgtttca
    59461 aaatggctgc catccttgaa aagacaagaa aaccagatgt caaatccaga ccaatccgca
    59521 tatgctggcc accgttttca gccatatatt cgattaactg gagccattcc cagagaatct
    59581 tcttatctaa atctgtcccc tgttgaaaag taggtagagc atgaaaaatc tcctctgctg
    59641 tctctttaaa atcatgttcc atcagtaaat atggatgacg attctctaac tggtacttgt
    59701 agcgatcctg taaaatatag cccttataga gatagacttg agcacaaagc tgactgagtt
    59761 cataggtgac atggtcctct gtataatccc ccaatagttc ctccttcttc atttcttgaa
    59821 tcaattgcgt cagtaaatct gccaactgcc ctccaaaacc aagaatatac tccatagtat
    59881 gaagaggaag aatgcgatga gataggagaa aagagaagaa tatcatcatc tcaccatcct
    59941 caactcctag tggaatatgt tgcccagcaa aaaaggacgg aacctttctc agcaaacgaa
    60001 ggtagaaaat attgtcaaaa taccctcgca ttttctcttc tatgacttga aactgacaag
    60061 cattgacccg aagacgttgt tgactgatgt gagcccagag aaccaagtcc aatttctgcc
    60121 ccacagacaa acttgcaccg cagatttcct ctaaattggc aatctcctgt tttctctctg
    60181 gtttctgcat gtgaccttcc cattcctgac tcgaccagac ctttcggaaa agacagaaat
    60241 agaaatagcg aatctgatgc tctggacctc gccaacgtcc attttggatg gataaatcaa
    60301 attctgacaa aatctgattt aaaccagcca agtgacgacc aagcgtagcc tcgctaatca
    60361 ccaattcttg agccagctga tgggctaaaa actgttggtg gtagagaaga taaaccaaaa
    60421 tctggtattt aacagcactc tccaaaaaca agctccgaat atctctcccc ttggtagctg
    60481 caccgataga cagacgcaga ttttcatctt ctgagtgaat agccagctct aagccactat
    60541 ccaaagcctt gtcattgagc agggtgacat atttggttag ggttgctttt gaaaatcctg
    60601 tttcttccat tacagccttg acggtcgtct gagactcttg taatagaaag gaaaggattg
    60661 aaaattgacc acactcggct ttctccatca aatctcctaa atacatttgt tacccctttc
    60721 attttctacc ttaagcatag cataaatctt acaaatgcta aaataatctg tttctctttt
    60781 tattttcatg ctgatttcct ggtccattat cctgaaaatc agtaaacaca cggctccccc
    60841 tttgggcatt tttatgctaa aatagtagct atggataaaa ttattaaaac tatatcagaa
    60901 agcggagcct ttcgtgcttt tgtccttgat agcacggaaa ccgtccgcac tgctcaagaa
    60961 aaacatcaaa cccaagctag ctcaactgta gcgcttggtc gaactcttat cgccagccag
    61021 attctcgcag ccaatgaaaa aggaaatacc aaacttacag ttaaggtgtt gggctctagc
    61081 tctctaggtg ctattatcac cgtcgctgat accaagggga acgtcaaagg ctatgttcaa
    61141 aatcctggtg ttgacatcaa aaagactgcg actggtgaag tcctagtcgg accttttgtt
    61201 ggaaatggtc aattcctcgt tatcacagac tacggtgctg gaaatcctta caactctata
    61261 actcccctca tctctggaga aatcggtgaa gaccttgcct tttaccttac tgaaagccaa
    61321 caaacgcctt cagcggtcgg cctcaatgtc cttttggacg aggaagacaa ggtcaaggtt
    61381 gcaggtggtt tcctagttca agtcttgcca ggagccaaga aagaagagat tgctcgcttt
    61441 gaaaaacgca tccaagaaat gccagctatc tctactcttc tcgaaagcga cgaccatatc
    61501 gaagccctcc tcaaggctat ctacggggac gaagcctaca agcgtctttc tgaagaagaa
    61561 atccgtttcc aatgtgactg tagccatgaa cgctttatga acgctcttgc cagccttcca
    61621 agctcagact tacaggaaat gaaagaggaa gaccacgggg cagaaatcac ttgtcaattc
    61681 tgccaaacta cttacaactt tgatgaaaag gacctggagg aactcattcg tgacaaatct
    61741 taatacacct tttatgattg gcaatgttga gattcccaat cgtaccgttt tagcgcctat
    61801 ggctggcgtg accaactcag cctttcgtac catcgcaaaa gagctcggag ctggactcgt
    61861 tgtaatggaa atggtctctg acaagggaat ccaatacaac aacgaaaaaa ccctgcacat
    61921 gcttcatatc gatgagggcg aaaaccctgt ctctatccaa ctttttggta gcgatgaaga
    61981 cagcctagca cgcgcagcag aattcatcca agaaaacacc aagaccgata tcgtcgatat
    62041 caacatgggc tgccctgtca acaaaatcgt gaagaacgaa gctggtgcta tgtggctcaa
    62101 ggatcctgac aagatctact ctatcatcaa caaggtccag tctgtccttg atatcccact
    62161 tactgtcaaa atgcgtaccg gctgggcgga cccatctctg gcagtagaaa atgccctcgc
    62221 tgctgaggct gcaggtgttt ctgccctcgc catgcatggc cgtacccgtg aacaaatgta
    62281 tactggccac gcagaccttg agacccttta caaggttgcc caagctctaa ccaagattcc
    62341 attcatcgcc aacggtgata tccgtactgt ccaagaagcc aagcaacgca tcgaagaagt
    62401 tggtgctgac gcagtcatga ttggccgagc tgccatggga aatccttacc tcttcaacca
    62461 aatcaaccat tactttgaaa caggagaaat cctacctgat ttgacctttg aagacaagat
    62521 gaagatcgcc tacgaacact tgaaacgatt gattaacctc aaaggagaaa acgtcgcagt
    62581 tcgtgaattc cgcggcctcg ctcctcacta cctccgtgga acatctggcg ctgccaaact
    62641 ccgtggagcc atttcgcaag ctagcaccct agcagagatt gaagctctct tgcaattgga
    62701 gaaggcatag tcttacatct acaactcaga aaaacgggaa agctcaaaat gaactgcacc
    62761 ccaaaagtta gacagaaaaa atctaacttt tggggtgttt ttattatgaa attaagttat
    62821 gatgataaag ttcagatcta tgaactaaga aagcaaggat atagcttaga gaagctttca
    62881 aataaatttg ggataaataa ttctaatctt aggtatatga ttaaatggat tgatcgttac
    62941 ggaatagagt tcggcaaaaa aggaaaaaat cgttactatt ctcctgattt aaaacaagaa
    63001 atgattaata aagtcttaca tgaaggctgg actaaagata gagtttctct tgaatacggt
    63061 ctcccaagtc gtacgatact tcttaactgg ctagcacaat acaggaaaaa cgggtatact
    63121 attgttgaga aaccaagagg gagagtacct aaaatgggac gtaagccaag aacgagacct
    63181 gaagagagga cagaattaga acgtcttcaa gcagaaaatg agtacctgag agcggagaat
    63241 gccatcctaa aaaagttaag agaactccga ttgaaggagg aaaaagagaa agaagaaaga
    63301 cagaaattgt tcaagaatta atgactgagt tttcgttagt tcttcttcta aaatccatta
    63361 aactagctcg ttggacctac tactatcact tgaaacagct agacaaaaca gataaggacc
    63421 aagagcttaa agctgaaatt caatccattt ttatcgaaca caagggaaat tatgcttatc
    63481 gccgggttca tttagaacta agaaatcgtg cttatctggt aaatcataaa agagttcaag
    63541 gcttgataaa agtactcaat ttacaagcta aaatgcgaca gaaacgaaaa tattcttctc
    63601 ataaaggaga cgttggcaag aaggcagaga atctcattca aggccaattt gaaggctcta
    63661 aaacaatgga aaagtgctac acagatgtga cagaatttgc cattccagca agtactcaaa
    63721 agctttactt atcaccagtt ttagatggct ttaacagcga aattatcgcc tataatcttt
    63781 caacttcacc caatttagaa caagtacaaa caatgttgga acagtcattc acagagaagc
    63841 actacgagaa tacgattctc catagtgacc aaggctggca atatcaacac gattcttatc
    63901 atcagtttct tgagggtaag ggaattcaag catccatttc acgtaagggt aacagcccag
    63961 acaatggtat gatggagtcc ttttttggga ttctgaaatc ggaaatgttt tatggctatg
    64021 agaaaacatt taaatcactt aatcgattgg aacaagctat tgtagactat attgattact
    64081 acaacaataa acgaattaag gtaaaactaa aaggacttag ccctgtgcaa tacagaacta
    64141 aatccttcgg ataaattaat tgtggttcta taatatttgt ag
//
LOCUS       DAVKWS010000006        50280 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKWS010000006 DAVKWS010000000
VERSION     DAVKWS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.006"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            601..897
                     /locus_tag="VW852_000416"
     CDS             601..897
                     /locus_tag="VW852_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0271608.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPYNW
                     GAFSNYYHGSQYHWAYVGSNERDNQKTVYAGAHSAAYAFVNTNLGERITFDAGW"
     gene            1000..3015
                     /locus_tag="VW852_000417"
     CDS             1000..3015
                     /locus_tag="VW852_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0271609.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFGALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLTI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNFITSTEWHDY
                     SPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEDHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3017..3649
                     /locus_tag="VW852_000418"
     CDS             3017..3649
                     /locus_tag="VW852_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271610.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            3790..4068
                     /locus_tag="VW852_000419"
     CDS             3790..4068
                     /locus_tag="VW852_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEV0271611.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(4578..4991)
                     /gene="ndk"
                     /locus_tag="VW852_000420"
     CDS             complement(4578..4991)
                     /gene="ndk"
                     /locus_tag="VW852_000420"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV0271612.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(5102..8779)
                     /gene="rpoC"
                     /locus_tag="VW852_000421"
     CDS             complement(5102..8779)
                     /gene="rpoC"
                     /locus_tag="VW852_000421"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV0271613.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPTVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(8812..12423)
                     /gene="rpoB"
                     /locus_tag="VW852_000422"
     CDS             complement(8812..12423)
                     /gene="rpoB"
                     /locus_tag="VW852_000422"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0271614.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            12839..12909
                     /locus_tag="VW852_000423"
     tRNA            12839..12909
                     /locus_tag="VW852_000423"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12871..12873,aa:Cys,seq:gca)
     gene            complement(13107..14438)
                     /locus_tag="VW852_000424"
     CDS             complement(13107..14438)
                     /locus_tag="VW852_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV0271615.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVYQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(14729..15553)
                     /gene="endA"
                     /locus_tag="VW852_000425"
     CDS             complement(14729..15553)
                     /gene="endA"
                     /locus_tag="VW852_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV0271616.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15592..15780)
                     /locus_tag="VW852_000426"
     CDS             complement(15592..15780)
                     /locus_tag="VW852_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0271617.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(15773..17056)
                     /gene="murA"
                     /locus_tag="VW852_000427"
     CDS             complement(15773..17056)
                     /gene="murA"
                     /locus_tag="VW852_000427"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0271618.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(17080..17310)
                     /locus_tag="VW852_000428"
     CDS             complement(17080..17310)
                     /locus_tag="VW852_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV0271619.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17368..18405)
                     /locus_tag="VW852_000429"
     CDS             complement(17368..18405)
                     /locus_tag="VW852_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV0271620.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18389..18877)
                     /gene="coaD"
                     /locus_tag="VW852_000430"
     CDS             complement(18389..18877)
                     /gene="coaD"
                     /locus_tag="VW852_000430"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV0271621.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(18867..19406)
                     /gene="rsmD"
                     /locus_tag="VW852_000431"
     CDS             complement(18867..19406)
                     /gene="rsmD"
                     /locus_tag="VW852_000431"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV0271622.1"
                     /translation="MKIISGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19471..20463)
                     /locus_tag="VW852_000432"
     CDS             complement(19471..20463)
                     /locus_tag="VW852_000432"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV0271623.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20576..21259)
                     /locus_tag="VW852_000433"
     CDS             complement(20576..21259)
                     /locus_tag="VW852_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV0271624.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21315..22055)
                     /locus_tag="VW852_000434"
     CDS             complement(21315..22055)
                     /locus_tag="VW852_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV0271625.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            22089..22367
                     /locus_tag="VW852_000435"
     CDS             22089..22367
                     /locus_tag="VW852_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV0271626.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            22445..23371
                     /gene="yidC"
                     /locus_tag="VW852_000436"
     CDS             22445..23371
                     /gene="yidC"
                     /locus_tag="VW852_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0271627.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPLLLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23446..24240)
                     /gene="pflA"
                     /locus_tag="VW852_000437"
     CDS             complement(23446..24240)
                     /gene="pflA"
                     /locus_tag="VW852_000437"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV0271628.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(24366..25616)
                     /locus_tag="VW852_000438"
     CDS             complement(24366..25616)
                     /locus_tag="VW852_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV0271629.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25683..26510)
                     /gene="purR"
                     /locus_tag="VW852_000439"
     CDS             complement(25683..26510)
                     /gene="purR"
                     /locus_tag="VW852_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV0271630.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(26777..27718)
                     /locus_tag="VW852_000440"
     CDS             complement(26777..27718)
                     /locus_tag="VW852_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV0271631.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(27708..28964)
                     /locus_tag="VW852_000441"
     CDS             complement(27708..28964)
                     /locus_tag="VW852_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV0271632.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(28966..29628)
                     /locus_tag="VW852_000442"
     CDS             complement(28966..29628)
                     /locus_tag="VW852_000442"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV0271633.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29591..30247)
                     /locus_tag="VW852_000443"
     CDS             complement(29591..30247)
                     /locus_tag="VW852_000443"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV0271634.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRLLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30258..31136)
                     /gene="rsgA"
                     /locus_tag="VW852_000444"
     CDS             complement(30258..31136)
                     /gene="rsgA"
                     /locus_tag="VW852_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV0271635.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(31138..32010)
                     /gene="rsmA"
                     /locus_tag="VW852_000445"
     CDS             complement(31138..32010)
                     /gene="rsmA"
                     /locus_tag="VW852_000445"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV0271636.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(32141..32449)
                     /locus_tag="VW852_000446"
     CDS             complement(32141..32449)
                     /locus_tag="VW852_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271637.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32442..33083)
                     /locus_tag="VW852_000447"
     CDS             complement(32442..33083)
                     /locus_tag="VW852_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271638.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(33085..35115)
                     /locus_tag="VW852_000448"
     CDS             complement(33085..35115)
                     /locus_tag="VW852_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0271639.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVHLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35192..35488)
                     /locus_tag="VW852_000449"
     CDS             complement(35192..35488)
                     /locus_tag="VW852_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0271640.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(35808..36707)
                     /locus_tag="VW852_000450"
     CDS             complement(35808..36707)
                     /locus_tag="VW852_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0271641.1"
                     /translation="MLIGQKIKEIRIEKGVSRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(36779..37339)
                     /gene="rnmV"
                     /locus_tag="VW852_000451"
     CDS             complement(36779..37339)
                     /gene="rnmV"
                     /locus_tag="VW852_000451"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV0271642.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLVGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(37339..38112)
                     /locus_tag="VW852_000452"
     CDS             complement(37339..38112)
                     /locus_tag="VW852_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV0271643.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(38408..39082)
                     /locus_tag="VW852_000453"
     CDS             complement(38408..39082)
                     /locus_tag="VW852_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV0271644.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKPETTLGVDKGGNTGSSD
                     ANANAGGGSQAGGPAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENTKTMEDVKEAETIGVQAIAMVTVLKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVAATSGFALLGRKTR
                     RRKWNS"
     gene            complement(39345..39479)
                     /gene="rpmH"
                     /locus_tag="VW852_000454"
     CDS             complement(39345..39479)
                     /gene="rpmH"
                     /locus_tag="VW852_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV0271645.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(39624..40838)
                     /locus_tag="VW852_000455"
     CDS             complement(39624..40838)
                     /locus_tag="VW852_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0271646.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(40994..41287)
                     /locus_tag="VW852_000456"
     CDS             complement(40994..41287)
                     /locus_tag="VW852_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271647.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            41449..41901
                     /locus_tag="VW852_000457"
     CDS             41449..41901
                     /locus_tag="VW852_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV0271648.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(41936..43324)
                     /locus_tag="VW852_000458"
     CDS             complement(41936..43324)
                     /locus_tag="VW852_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0271649.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            43390..44352
                     /locus_tag="VW852_000459"
     CDS             43390..44352
                     /locus_tag="VW852_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV0271650.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(44558..45568)
                     /gene="ccpA"
                     /locus_tag="VW852_000460"
     CDS             complement(44558..45568)
                     /gene="ccpA"
                     /locus_tag="VW852_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV0271651.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(45993..46592)
                     /locus_tag="VW852_000461"
     CDS             complement(45993..46592)
                     /locus_tag="VW852_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0271652.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(46594..47691)
                     /locus_tag="VW852_000462"
     CDS             complement(46594..47691)
                     /locus_tag="VW852_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0271653.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGELPAWHLLVDILFVV
                     TYLGVLITKNQRISWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(47691..48428)
                     /locus_tag="VW852_000463"
     CDS             complement(47691..48428)
                     /locus_tag="VW852_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271654.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(48430..49314)
                     /locus_tag="VW852_000464"
     CDS             complement(48430..49314)
                     /locus_tag="VW852_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271655.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(49301..49495)
                     /locus_tag="VW852_000465"
     CDS             complement(49301..49495)
                     /locus_tag="VW852_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271656.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(49492..49698)
                     /locus_tag="VW852_000466"
     CDS             complement(49492..49698)
                     /locus_tag="VW852_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271657.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        14533 a        11422 c         9582 g        14743 t
ORIGIN      
        1 aacaaataat tctttatggt aataaataaa aaaagtcaaa aattacataa aaatacttga
       61 ccgaataata tatatatgtt aaaataaaaa caaggaaaaa gaaaggggtt tcacgaatga
      121 agaaaaatcg tggaattcaa aaattagcta tattagtatt actaggtgtt tttatgttta
      181 gtaacacaat tccttaccaa cagttattca gaagaataga caattggaga ttcgagtgca
      241 atcacaaaag aagtccaatg gtcttgatgt tgggaaggct gattgatttc gtattacttt
      301 aaaattcaac tatttagaga aaggtattct gatgagagct tgtaacaata ccagatagat
      361 ataagaatgg agatgtttga taaaatgctt tcatagctaa ccaactgttg aaaattatga
      421 gttacaaaat ctaaagtatt taagattggt ttataaaatt aggtctctgc ctgtagaaag
      481 gaacgtgcag ttagaaagga ggaaaaaata ggatactata attcagactg agatttctaa
      541 ttaaaatatg tagttgcatt tcgctcattt tcataatatt tcatttagag gagtaataat
      601 atgttaaaat ttagaaaagt agttgcatca tcccttgccg ttttggcatt tagtttggct
      661 cttcctgttt tggcagtttc tcaccgtggt ggcgagtgga cctatggagg ccatcatgat
      721 ccatataatt ggggagcatt ttcgaattat tatcatggtt cacagtatca ctgggcctat
      781 gttggaagta atgaacgtga caatcaaaaa acagtttatg caggagcaca tagtgcggcc
      841 tatgcgtttg ttaatacaaa tttgggggag cgaattacat ttgatgcggg atggtaaaga
      901 ataattgaag aagattcttt tatcaatagt ttagtaagct tgttagttta tagagggcag
      961 gggtatttct gccctctgtt ttagaataag gaatgcacta tgaaaaaatt atttatactc
     1021 atatccaacc tgcttgctag tttgttcttt gtttgggtat ttaccatttg gactgataca
     1081 tatgtctccc attattaccc caacgttgtt gtacatgatt cttcccctga aacaactttt
     1141 cagcatgttg ctacacgctt ggaaaaacta gcagaagaaa cagatagctt tattgcgatt
     1201 caacaccaag atcctaactc agaaggtact acagtttttt cttatacgac ctttggagac
     1261 ggaaagttgc ctgacgggct acaggaaaaa aaactagaag atgctcaaag tagtagtgtt
     1321 gaaacaaact attttgtatt cgatggacac ctagatattc acttgctaag ggaggagcta
     1381 agtcaacttg gcttgactaa tatgcacctg acaattccat ctaaactatc tacgttaatg
     1441 gctatcttta gtaacggatt tcagttaatc agtctattga ttttcatctt aacctttgga
     1501 gcactgactt taattagtca aattagccaa ttacggtcgt cgggcattcg cctcatttca
     1561 ggagagaaac ggtggtccat ttttcttaga ccagttggtg aggatttgaa agggatagct
     1621 gttggtttca gtttagctgg cgtgctcacc attcttatgc aaaaaattct gtcgcttcca
     1681 acgcaatccc taatgaccat aggagcaggc ttgctcagtt ataatctcat cttgttgtct
     1741 atctccctgt ttttcgctca actcttcgca gttgggataa aaaagataca cctgatgcag
     1801 attataaaag ggcaagtgcc tgtcagagga atcattagtt tgattttaat cggtcaacta
     1861 ttagcgatta ttattgtaac actgggaata gggagtagct taaagtattc ccaagcctgg
     1921 caacagcatc ggattggaca agaggcttgg agccaggaaa ggcaactgat taccctatca
     1981 atcagccgtg aaggaacgag tcctggtttt gatgaacaag ctcaaaggaa actcagaact
     2041 tggtatcaat tgatggatct ggctgtttca gaacaaaagg ctttcttatc tagacaccag
     2101 ttaattgacc gtactttgca aaatggcatg gcttcctcca aaaactttat aacctctaca
     2161 gaatggcacg attacagccc gaatggcaat gtccttatcg tcacaccgca atacttggag
     2221 cgtcaaaaca ttcctgtaga tacaactatt gagcaaaaga tgaatcacct tgatgtaggg
     2281 gagtttgtct tattgctgcc tgaacacctc cgttcagagg aagatcatta taaatctgtt
     2341 tttgaagacg acttaaccag tcgcatgtct agtcaagatg aacgacagca aatgactgct
     2401 acggtaggtt atttagaatc aggtcaggat cgttttgtgt ataatacgac ccctatttct
     2461 taccagcagt ttttgaaaga tccaatcatc attgttataa caccccaatc aactggtcca
     2521 cagtccattt tgttttggat agacgcagta cagaactacg ttctctttaa tcaattgtct
     2581 gatgcccagg agcttatcca gagacaaggc attgaaaatt gggtctcaga aatgcaaaca
     2641 ggttaccaca actacatcac attattggat aatatccaga gggaacgttg ggtaatgcta
     2701 gcaggagctg tgcttgggat tgcaacttca atcttgctgt ttaacactat gaataggctc
     2761 tactttgaag aatttagacg tgccattttt atcaaacgca ttgcaggtct caggttctta
     2821 gaaatccatc gcacttatct ctttgctcaa ctgggtgtgt ttttactggg atttgttgct
     2881 agtgtatttc ttcaggtaga aataggagtt gctttcttag tcttgttact ctttactggt
     2941 ctatctcttt tacagttaca tgtccaaatg cagaaagaaa acaagatgtc catgcttgtt
     3001 ttgaagggag gttaatatga ttgaacttaa acaggtgagt aaatcttttg gagaacgaga
     3061 gttattttcg aatctttcaa tgacatttga ggctggaaaa gtctatgcct taattggttc
     3121 aagtggtagc ggaaaaacaa ccttgatgaa catgattggg aaattagaac cttatgatgg
     3181 gacgattttt taccgaggta aagacttggc caattataaa tcgagtgatt ttttccgtca
     3241 cgaattgggc tacctcttcc agaactttgg cttaattgaa aaccaaagta ttgaagaaaa
     3301 ccttaagcta ggtctcattg gtcaaaagtt gagtcggtcg gaacagcggt tgaggcagaa
     3361 gcaggcttta gaacaggtcg gcctggctta tcttgaccta gataagcgca tctttgagtt
     3421 atcgggcgga gaatcgcaac gagttgcctt ggcaaaaatt atcttaaaga atccaccctt
     3481 tattctggca gatgagccaa cagcttcaat agacccagca acctctcagt tgattatgga
     3541 gattttgcta tctcttcgag atgataatag gctaatcatt atcgcaacac ataatccggc
     3601 aatttgggag atggctgatg aagtgttcac gatggatcat ctgaaataaa aatccttgtt
     3661 tttaattgca cgatgagtta ctgaaatatt atcatgaatc aagaattgga gttaatttag
     3721 aattgtactt tattaatatt gaggtaactt tttcttgata aaggaggaaa tagtggagag
     3781 gaagttagaa tgaaaaaatt cgacaattat attattgaga agccttgcga ttctaattca
     3841 gataaactgc aaaaaatctt aataattgaa aatttggtag atgatatttt gcaatttcct
     3901 ctcagaatca ataatagtgt aggagagatt ttcctcctac aaccgtttca aaagaaaact
     3961 atctttattc catgttattt tgaggaagat attgtgaaag tcaaagatga tgataaagtt
     4021 gagtggaatt tgttagaatt tcaaaaattt agagcatttt tggcttagta atctgtgttg
     4081 aaggctcaaa acctatggta aaaaagtcgc tttgaaaacg tattgcctcc aaagatttag
     4141 ttaaataatg atttaacaca aaaagaaatt attgaagttc tggaaagatg ttgtttcagt
     4201 attgagaaaa ggtgggaaaa acttgcgatt ttcacagaga aaggaagaaa aagtatagaa
     4261 atatagtcaa ttgaaacaag aacaggacaa aagaaccttt tgtgccatat ttttctcctt
     4321 tcgctttaca attggcttga acacctttat tgtatcgcgt ttggagtttt tttggtataa
     4381 ccttcgacgc gcacccgcat agcgggtgtt tttttgtctc gcacctaacg gagcgagacg
     4441 aactaatagt cacttaatca aaaaaacgca ccatatcaaa aactaaaaag tttgatatca
     4501 tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt taaccaattg
     4561 attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga ctcttctgaa
     4621 tctgaaccat gtacaacatt ttggataacc tcattttctc cagcagcttt tgcaaagtca
     4681 cctcgaatag tgcctggtaa agcttcttct ggacgagttg cacccatcat ggtccgccaa
     4741 gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga agtcatgaat
     4801 tcacgaatcg gtaggtaaaa actctgacca accaagtcct gatagtgctg gtcaatcaac
     4861 tcttctgaaa cctgtgaacg aaactccaat ttttcgattg taaatccacg ttgttcgatg
     4921 cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat gataaagaat
     4981 gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca catttcgtgg
     5041 aaaaatggag aaagttttca gaagagagaa tgagagaacc cgagggttct ctcattctct
     5101 cttattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact tctgttgttt
     5161 cttcatttcc ttcttcctct actgtaggat taaggtattc ttcttcgttg acagcatgtg
     5221 gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta ccgatgataa
     5281 cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg tcagtaagga
     5341 cacgagttgt ttcttggaag gaagctgctg acaagaaact gtttgtttca agtgaggctt
     5401 tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg ataaggacat
     5461 ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga agatctgtat
     5521 cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc tcgatgtgtt
     5581 tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg agaaggtacg
     5641 tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata gaaccttctg
     5701 tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga gctgtaaatg
     5761 gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg gtacgagttg
     5821 atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtgataacc gcttcccctt
     5881 taggattacg ggcttcaaag atttcttgga cacgaggaag accctgagtg atatcggtat
     5941 ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca ggttccccga
     6001 tagattgggc agcaattgta ccaactgctt caccaacttc aaccgcatca ccagtcgcca
     6061 agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta aatacagaac
     6121 ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct tctgtaatca
     6181 attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt ttcttagtgt
     6241 aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct gcgatagaac
     6301 ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg tcttgggcaa
     6361 cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc gtatcggtca
     6421 tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa ccttcgcgga
     6481 agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc agaccacgca
     6541 taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc atcatcataa
     6601 cgattgggtt cttaggatct tggttagcaa tcaagcgttt ctcaagtttt tcacgggcag
     6661 cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata ccacgacgga
     6721 attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca gtcttgtcat
     6781 caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag tggtggtaac
     6841 cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa cgtttgaaga
     6901 tttcagcgat gatatttcca aggtttttct tcttgaatgg agggttgagc tcaagattgc
     6961 tgatagcttc cttgatatct ccaccaagtg gcaagaagta tttagctgga acaccttctg
     7021 tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg atatcgttga
     7081 agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct gtccaaggct
     7141 tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa ccattgcggt
     7201 aagccataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg cgaccagctt
     7261 cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta actaccggtt
     7321 tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga gcttctgctt
     7381 gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag tcagcattgt
     7441 aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaattaag actggctcga
     7501 aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact gggtgttctt
     7561 taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc aagcgtttag
     7621 ctgctttgac gttttgcacg atatcacggg caacgatttc acgcataaca aatggtttaa
     7681 agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga gttggaccaa
     7741 cggcgataac ggaacgtcct gagaagtcaa cacgtttacc gagcaagttt tgacggaagc
     7801 gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta cctggtcctg
     7861 tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct tgaagcatac
     7921 gcttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga gccaaacggt
     7981 tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca aaacggccac
     8041 catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg ttaagaatca
     8101 tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa cgacggatgg
     8161 ctttgacacg tttttgtcca gtagctgttt tcaattcttc tttgagttca gcaatttctt
     8221 tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc ttggcaacaa
     8281 atgaaccata accatattca cgcaagcgct ctcggtattc gcgctctgtc atgatagact
     8341 tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag tagataactt
     8401 cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga atccccttga
     8461 agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc tcacgacgaa
     8521 ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg taacgaatgc
     8581 gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc acttcatcaa
     8641 agagtccttc acgttctggt ttcaaggtac ggtaattgat tgtttcaggt tttttgactt
     8701 ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc atacttttaa
     8761 aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg cttattcttg
     8821 ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt cagcttcaaa
     8881 ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat ctacgtggat
     8941 gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt catcttcgtc
     9001 taggacacgc atgtcaagac caagagattg caattctttg acaagaactc ggaaggattc
     9061 tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg ctttcaaacg
     9121 tccgttgata tcgtcagact tgtaagtcaa gatttcttga aggacatttg acgcaccgta
     9181 ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact gagctttacc
     9241 tccgagtggt tgttgggtaa cagttgagta aggtccgact gaacgcgcgt gcaatttatc
     9301 gtcaaccatg tggtggagtt tgatcatgta catgactcca acagaaacac ggttatcaaa
     9361 tggttcacca gtacgtccat cgtaaaggat cgttttggca tcgctatcca tacctgcttc
     9421 tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg tcgcaatgtg
     9481 aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac cgatattcat
     9541 acgtgatggc accccaagtg ggttcaacat gatgtcgact ggagttccgt ctggaaggta
     9601 aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt gacgtccggc
     9661 cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa ccaacatgtt
     9721 aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat cacgaacgac
     9781 accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag acttgtctcc
     9841 aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct taggtgttac
     9901 tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa tccccatttc
     9961 gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga tttcttcagg
    10021 cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag atgtgtagac
    10081 atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt aaccttccca
    10141 agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt ccatagaagg
    10201 tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacga gagtgcgttg
    10261 gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa catccaatga
    10321 accatcttca cgacgaactt ctaccttgtc agcatctgcg taagtaactt taccatcata
    10381 ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac cagtaccaac
    10441 gtaaggtgcc tgaggattaa tcaatggcac agcctgacgt tgcatattgg ctcccatgag
    10501 ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac
    10561 ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt ggttgacccc
    10621 ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttcattca gacgagagtt
    10681 agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa tttcgttcgt
    10741 gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac catatttgtt
    10801 caagtgtccg taagatgaca agttattgat caaaccgatg ttaggtcctt caggtgtctc
    10861 gattggacac atacgaccat agtgagtgta gtgcacgtca cgtacttcat atccggcacg
    10921 gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag acaactcaga
    10981 aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga attctttaac
    11041 tgcagctgtt acaggacgga tattgataat ttgttgtggt gtcaagactt cattgtcctg
    11101 aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac gtacttggtt
    11161 ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga tatcatctac
    11221 acggccaagt ccttcagcca agttgaggaa gtagctcatc tcggcaagga tatctgcagg
    11281 agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta cgacgcgatc
    11341 tggatcagtt ggagcaacaa ccttgaattt ttgaagaaca acaggctcag tcacaacggc
    11401 tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc tttcaatgct
    11461 ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc cagtttcagg
    11521 gtctaccaat ggctctgcaa tggtttggtt gagcaaacgt gttttaacat tgagtttttt
    11581 attgattttg taacgaccaa ctgctgccaa gtcatagcga cgtgggtcaa agaagcgagc
    11641 cacaagcaag ctacgtgagc tttcagctgt cttaggctca cctggacgaa ggcgttcgta
    11701 aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga tatctttttc
    11761 aacagtgttg cgaaccaatt cgctgtcacc aaagatatca aagatttcat catcacctga
    11821 gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt cgatacgagt
    11881 gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt tagggataac
    11941 agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt aaacacctgg
    12001 tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga aagtacccat
    12061 ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt cgcctgtttc
    12121 tttattgatc aaacggaagg ttacaaaaat tggtgctgag tagctagcat cgtggatacg
    12181 agcttcttct agcgtgtatt ttggttcctt gatttcatat ccaacaaatt ccaactccat
    12241 tgtgtctgtg aagtttgaaa ttggcaatac atcttcaaac acttccttaa gaccgtggtc
    12301 taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt caagaacttc
    12361 tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt catgtcctgc
    12421 caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt ttctaatcgc
    12481 catatggttg taaacccctt atcaccgtgt cctcttgacg aattttcaga atctttaagc
    12541 ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct aaatctgact
    12601 ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac aaaacctacg
    12661 acaaatgatt acccatatta taccctattt agctagattt ttcaaggggt ttcagtaggt
    12721 ttttggtaaa ttttttccca tagaaaactt ggcatcacat tcgaatcacg ctatggtaca
    12781 aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata aacgttaagg
    12841 cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt tcaaatccgt
    12901 ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc caatttgaag
    12961 ggcttttctt attttccctc gaataaatac gtataacttt aaaaactttt ggagcgagtt
    13021 tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa aaagcttcgt
    13081 cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc gacttctgtg
    13141 atttggattt ttaccttggt aacacgtcca ttcttcactt tatcattggt taagataaac
    13201 tgtttgtttt ggctgatcaa ttcatagctg agtttctcag tcgttggaat ggttcccact
    13261 cctgtcaaat aataaccagc gatagtatca acgtcatcac tttccagttc aacattaaag
    13321 taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc ctcgccaatc
    13381 tgataaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac aatttcctct
    13441 aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag caagattgcc
    13501 atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat agtttcaggt
    13561 acaaaaagtg gatcttgtaa aattctcttc caaacaatat tgtcaaaacc gtccacaaag
    13621 cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt atccccatca
    13681 taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc ttgactatca
    13741 tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg tgccatcagt
    13801 tcatcgagcg aaaagacacc ttgtagcatc tcaatctcat cagcatccaa tgtttcttca
    13861 ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc atcatcgaat
    13921 gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca aacaaaagga
    13981 ctgactagtt ttccgatccc aatgataacc ggcgctgtac gaattgccaa ggcatccttt
    14041 agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat ataggtcaaa
    14101 aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc aagccaagag
    14161 gcaatcacac gtcctagagt atcagttaaa ctcgcccctg ataagattgt aatcagggtg
    14221 attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt cagcaggcgg
    14281 atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt aagagaaacc
    14341 atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa caataaaaca
    14401 aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct aaaatagtat
    14461 cttatccttt attatatcac aaaatttaat ccaatacata ttattttttt cttagcgtaa
    14521 cttccatctc tctcccaaaa ctggaagtta cgctaagacg gatttccact gatttcacgc
    14581 gtttttctca tactaaaagc taggttgtgg gaaactggaa gttagtttta gaagttgcta
    14641 ttttttctta gtacctgttc tagccctgct taaactagta aaaagaactc ctacatccat
    14701 aagtgacata ggagtttagg cttatctttt actgagttac agttacttct ccagttcggt
    14761 aatccagttg aagtcccttt tgaacattgg gaactagaac attgaattcc aattctccat
    14821 ccgaagactt ggcttcaatc tgtgaagctg agggaactaa atcctcgttt gaagcgtagt
    14881 aaagggttac acggtaacgg acacgcttgt tttggtccaa ggctttacgc accttgcttt
    14941 catagtagtt ttgaccagtc gaatactcgg cttgtgcctg atttgcccag gctgtctgaa
    15001 cagcaatgtt tttaggattg cttgttgagg catcaaaacc atccaaacca ccgattaagg
    15061 catagcctaa caaatgacct ctatcgactg catgggtata agagcccttt agattcttga
    15121 cctgatgcca acctggagga gtccaagaag ttgaaccatt cccagtttct ttacgattct
    15181 tgtactgacg agtggcatta gacaagaggg cattagctac ggttggaaca gtttccttgc
    15241 ccactgtctt tgttttattg tcagcgtagg gcttacttga aaccttggca tctagatttg
    15301 ttttattacc attgacgata aaagcacctg agccattcca ctccagactc ccctttattt
    15361 gactcttgac tgcgtctgtt aagacactct ctgccaatgc ttgactagga gcttcaggcg
    15421 cttgtttttt ctgactaaga ttggttttgg gactattagg tgccgactgc atctgcttga
    15481 tataatagct ccctgtagac aaaagcaata acactagcag tccgattagt gtctgtcttg
    15541 tttttttgtt catatttctc cttatcttta gaaaaaggct ggttctccag cctaatttcc
    15601 tgtaaattta tgaatcaatt cctgccattt tgctggagac aggatagccc atggatcttg
    15661 acccttgccc aagattccat aacctaccat tagaccgatt cctagagcca gagtacctaa
    15721 aatcagtact atgatgacta aaagtaaacg cttgactacg tagcttgatt tcttattcat
    15781 cttcatcact tgcctcaatc cgctgaatct tagcacctag ctgcgccaac ttctcatgga
    15841 aaccgtagta acctctatcc aagtgaacca atttaccgac cacagtttct ccctgtgcta
    15901 ccaaacctgt caaaatcaaa gctgcactgg cacgaaggtc agttgaaaga acttctgctc
    15961 cctgcaaagg ctgtccacca acaatacgag ctgtatcacg gataatctca gaatgcaagc
    16021 ccatgcggcg catctcttct aggtgttgga aacgattttc aaaaactgtc tccaccatgg
    16081 ttgattcgcc ttttgcaact gtcatcaagg ctgtaaattg agcctgcata tctgttggaa
    16141 atcctgggtg gggcaaggtt ttcacatgaa cagcttttag attttccagt tgagaacgaa
    16201 cacgaattcc ttcgtcttct tcaattactt caacacccat ttcaagtaac ttggcaatca
    16261 agggacggtt gtgctcccag acagcgtctc gaatcaagac atcaccacca gtcatggcag
    16321 cagctaccat aaaggttcct gcttcgatac ggtcctggac tacattgtga gtcgtaccat
    16381 gaagtttctc aacaccagta atggttatag tctctgtacc agcacctttg accttggctc
    16441 ccatttcatt aaggagaatg gctaagtcaa caatctcagg ctcacgcgca gcattctcaa
    16501 tcactgtcac cccatcagcc agagtcgctg ccatcatcaa gttctgcgtt gcaccaacac
    16561 ttggaaagtc catatagata tgagcaccat gcaagcgttc tgccttggct tcgatgtaac
    16621 cagctgtctg actaatctta acccccatag cttccagacc tttcaaatga agatcaatag
    16681 gacggctacc aatcgtacaa ccacctggca tggatacctt ggcatgaccc acacgggcaa
    16741 ggattggccc taatacaacg atggaggcgc gcatcttact gacatacttg taaggggctt
    16801 cctcagtgat gtcgccagta gcatccacct tgacaagatg agcttcctca tcaaagtcaa
    16861 ccttggcatt caaaccacca actacctgat tcataataaa gacatccgac aaaatcggaa
    16921 cattctgcaa gacggtcttt ccttcacttg ctagaatagt cgctgccaac aagggtaaga
    16981 ctgcattttt tgctccctcg atcgtcacgc ttcctaccag acgattatcg ccaccttgaa
    17041 ccacaatttt atccatactc gtttccttta ctcttgattt tataataatc ctctaagcgc
    17101 ttcttgccac aaatgataca ggctaataaa gaaagaactc aggatgtagc ctatgacaat
    17161 gctaaaaaag gctactaata cacgaacttt tcttgtattc tcagctgtca cttttaaaac
    17221 cttttcccat ctaacaagat cctttaaaag gtaaaaactc acataaataa agagcatatg
    17281 actacttaga gtgaataata attgaaccat ttgactatta taccatcttc tctgtttgtg
    17341 cgctagtttg gaaacttcac tcaaaaatta gggattgttt ttcaagtaat caatcgcatc
    17401 ttgtagggtt ttaacgggca cgattttcat atccgtcttg attgttttag ccgcttctag
    17461 ggctgtttgg tagttgtttt tcgcgtccgg atgcgccttt tgttcttctt cgctaacagg
    17521 gttatcagga gcaaagaaaa tagcagcacc tgccctagcc gaagctacaa ccttcttatc
    17581 aatacctcca atgtctccca cattaccatc gcggtcaatg gtacctgtac cggcaacaat
    17641 acgaccatta cgaagatctg ggtgagctat ttgagtatag atagctagac taaacatgag
    17701 accagcactt gggccgccaa taccagctgt tgaaaagcta attgggacat tgctgattac
    17761 ctctgtacga tcaatcaagc cgattccaat cccatttttt ccattttcca aggtgataat
    17821 ctttccttct gcagacttgg tttgcccatc ctcttcatag gtgaccttga cggagtcccc
    17881 taatttttga gaactgacgt aatcaatcaa gtctttggaa ctatcaaagg tctgatcatt
    17941 gactgctgtg actgtatcag agatattgag aatcccttta aaggttgaat tatccgtcac
    18001 attcaaaaca taaactccaa agtacttgag ttcgatatcc ttaccagctg tttttagtcc
    18061 ttgatacttg gccatatttt gcgatgtttg catgtagaat tgattgattc gcataaattc
    18121 aacatcggaa gaaccacctg tagtctcctg agcactacga atatctgtaa aaggtgtcaa
    18181 ccaagcataa atcatatgag ctaaagtagc atgttgaaca ccaaccgtaa cgaattgata
    18241 ggcaccagct tccttatctt ctgtgtcatt tactttaagg acttggcgaa tatcttccga
    18301 accacctgga acctctatat agtagggcaa aggcaccaca aatgccaaga aggtcacaat
    18361 caaggccgca atgacataca agggccatct aatctttttt ttcatttctt atttcctcca
    18421 aaatactctc gggaacatgg caggcaatat cctgaccaaa cttcaaaagc tctcgaacac
    18481 ctgatgaact gatatagaga tgttcaggcc ggctatgtaa ataaatagtc tctatatcag
    18541 aagacagctg atgattgtag taatcaaaac tagcttcata ttgcaaatcc gccgcattcc
    18601 tcaacccccg tactaggaaa gtagccccca gtctttttgc aacatcgacc accaatttat
    18661 catgagaagc cacgacttca acattttcca aatgtcccaa agccttttct agcccccgtt
    18721 tacgattttc aagagggaga aatccttgtt tgtggggatt aaaaaaaata cccacataaa
    18781 gcttatcaaa aagtctgctc gcccgttcaa tgatatccag atgcccattt gtcatcggat
    18841 caaatgagcc tgtgaataag ccaatcttat ctgacataga ctgtcacctt actaattcca
    18901 taaatctttt ccttccagat acccagacag gcaatttctt ctggaagttc aacggcttta
    18961 tccgtctcgc acacaaccat aacatcttca gaaaaaagct ctctctcagc cattttttca
    19021 atatctgcta cgatttgttc cttggcatag ggagggtcta agaaaacgag gtcaaattcc
    19081 ccagatacct gttccaatgc cctttctgca tccattttga ggagttgaaa ttttccaact
    19141 tccttggtca tctggatatt ttcagccacg atggtctgag ccttacggtc tcgctccacc
    19201 aaaacagcac tggacatgcc acgcgatact gcttcgatag ataaaccacc actacctgca
    19261 taaaggtcca agactcgtcc cccttcaaag tagggaccaa tcatgttaaa aatggctccc
    19321 ctaaccttat ccgaagtagg tcttgttgtc ttgccttcta gtgtcttaag gggacgtccc
    19381 ccatagattc ctgatatgat tttcatactg tttattatac caaattatag acaaaaagag
    19441 aaagaaaacc gaaccttgcg gttcgattct ctacaaaata ttttcgtaag tatcgcggac
    19501 ttcttgaggc caaacacttg tttgcacttc tccgatgtgt ctcttgcgaa gtaggaacat
    19561 ggccatacga gattgtccaa ttcctccacc gattgtcaat gggaataggc cattcaacaa
    19621 agacttgtgc cattccaatt ccaagcggtc ttcatcacct gtaatttcca cctgacgtct
    19681 aagagtttct tcatctacac gaattcccat agaagacaac tcaaaggctc cacctaaaga
    19741 ctcattccag acaagaatat caccatttag acccttgtag ccattctcag actcgcttgt
    19801 ccagtcatca tagtctggtg cacgtccatc gtgcggttta ccatctggca actcgccacc
    19861 gataccaatc aaaaagacgg ctccaaattc tttacaaatc gcattttcac gttctttcgg
    19921 tgtcaagtct gggtagcgtt ctaccaattc ttctgtatgg ataaaggtga tttgttttgg
    19981 caagatagac tcgatgtcat agcgggcttc aacagctagc tcagtcaggc gaatagcctt
    20041 ataaatcttc tcaactgttt cttttagata aacgatgtta cgcttaccat ttgggataac
    20101 cttctcccag tcccactggt caacataaac agagtgggtt gcatccaagg aatcctcatc
    20161 tggacgaagg gctttcatgt ggacaaagag accctctcct tcaccaaagc caaaacgagc
    20221 caaggtgtgg cgtttccatt tagcaagtga gtgcaccact tcataagtag catcagggat
    20281 ttggagaacc ttgaccgata ctggattttc cacaccagac aggttgtcct gcattccgtc
    20341 accgacctta ctcaagatag gaccttgaac ttcgacaact tctagcttat ctttcaaata
    20401 ctgggtaaaa gtgtttttga caaaggaaat ttcttcttgt tgatggataa aacttttctt
    20461 catagacaac tcctcaaaaa tttaataaac gtattataca cctatttcca agaaaagaaa
    20521 agagaaaatt taaaaaaaaa tcagtgtcac tcaaaacact gatttcatag accttttagt
    20581 cattgcgacc aaagatacgt agaatactta ggaaaagatt gataaaatca agatagatgc
    20641 tgagagccat tgacaccacc cagcctgtcg ctacacgacc ttgtgattgt tcataggcga
    20701 ggcgaatctt ttggttatcc caagcaatca accctgaaaa aaccaaaacc atggctacgc
    20761 taatcatata atcaaagaag ccactagcca agaaaatatt gactaccata gcaatcagta
    20821 aaccaatgag agctgccatc attgctcgac caatcccact caagtctttc ttggtaaaga
    20881 taccaactgc cgccatgaca aagaagagaa gggcgctcga tacaaaggca gataaaactg
    20941 tacccggagt atagaaagcc accacaaaac taagggtaaa gccgtttaaa acggagtaaa
    21001 gtaaaaatac tggaagagca gctggactat ttcttgaagc catactactg gcaacgaaaa
    21061 ccaaagccaa ctccgcaaaa gtagcaatgg tcaaccagag acgcccctgc atcaacaagt
    21121 aaaccaactg agactgaaag accgtcaaca taaggcctga taccaaagca gatagtccga
    21181 ttcccagacc aacaaaggca taaaccttag cgtaaaattg attgagacct gcacggtcat
    21241 gtataatagt gtgattcatc cttttctcct tttctgtgaa catctttcct tgattataac
    21301 aaaaaaagtt aaaattagct taaatggaag attaaaatac cggctgcaac tgcaacattc
    21361 aaactctcgg cctgcccctt catgctaata tggaccaact ggtctgcact ttcagccata
    21421 aggggactaa ttccttgacc ctcatttccc ataactagta caaaattttc tatagaaggc
    21481 agttctctgt aatcaacaga atctttagat agggttgttg ctagcactgg gatagctacc
    21541 tttttagttt cgtcaagaag cgcttgacta gtcatccggt aaatgggcag atggaaatga
    21601 ctaccttgca tggaacgtag ggttttgaga ctgtagatgt ctgccgactt atctgaaaca
    21661 atcactcctg taaaacctgc tgcatccgca gttcgaatga tagttcctac attaccagga
    21721 tcttgcacat cttccaaaaa caagaacttg ccctgactca aatcagcttg tcctacttct
    21781 tctttttgaa ccaccgccac aattccctgt ggagtctgag aatccgccaa atctagcaaa
    21841 atatcctctg aaatccagac agtttgcgga aaagcagcta actgatctcg ataattttct
    21901 agggcaaaga ttttctcaat cgtcactcca gcttggacag cctcttcaaa caagtgccag
    21961 ccttcaatca aataggcaga cttgcggtat tttttttggt gtaatttctt ggcatttttt
    22021 accacagaat tggcttttga ggttataata gtcatagaaa tattataaca caatcaaagg
    22081 ggtttggtat gcaaaaggtt agaatgattg cccaaggtag ggtacaggga gtcggctttc
    22141 gttggggtgt ttacagcttg gcacttgaaa ttggtggcat cacaggtcga gtatggaata
    22201 acgacgatgg cacagtggaa atcttagccc aagcagactc atctgctatc atggcaaaat
    22261 ttatccaaga aatccgaaaa ggaccgacac ctttttcaaa agtaagctac ttagatgtca
    22321 aactaagcaa ctttcctccc tactctgact ttaaaatcgc aaattaggtc tccagaacta
    22381 ttgtatattt tgtaaaaaaa cagtagaata gaaaggtata atttttaaag aaggaataaa
    22441 aacagtgaaa tctattaaac gttttgcatt ctcggctatg ggagtggcta tgttgcttgt
    22501 cttgactggc tgtgtcaatg tcgataaaac cacaggtcag ccaacaggat ttatttggaa
    22561 taccatcgga gcgcctatgg ctgaagccat caagtacttc gctactgata aaggtctagg
    22621 ctttggtgtc gctatcatta tcgtaaccat tatcgtacgc ttgattatct taccacttgg
    22681 tatctaccaa tcatggaagg caacgcttca ctctgaaaag atgaacgccc tcaagcacgt
    22741 ccttgagcca caccaaacgc gtctcaaaga agcgactact caagaagaaa aactcgaagc
    22801 ccaacaagct ctctttgctg ctcaaaaaga gcacggtatc agcatgtttg gcggtgtagg
    22861 atgtttccct ctcctccttc aaatgccttt cttctctgct atctactttg ctgcccaaca
    22921 tactgaaggg gttgctcaag caagctacct aggcattcct ctaggttctc caagtatgat
    22981 tttggttgcc tgtgctggtg tcctttacta tcttcaatcg ctcctttcac ttcacggagt
    23041 agaagatgaa atgcaaagag aacaaatcaa gaaaatgatt tacatgagcc cactcatgat
    23101 cgtcgtcttc tccctcttct caccagctag tgtcacactt tactgggttg tcggtggttt
    23161 catgatgatt ctccaacagt ttatcgtcaa ctatatcgtt cgtccaaaac ttcgcaaaaa
    23221 agtccgtgaa gaactggcaa agaacccacc aaaagcaagt gctttctcaa caccaagtgg
    23281 acgaaaagac gttacccctg aacaaccaac tgctattaca agcaagaaaa aacacaaaaa
    23341 tcgcaacgct ggaaaacaac gttcgagata agaagaaaaa ggcttagctg atttgctaag
    23401 tctttttgca agtcaaaagc ccgatgtttc catcaggctt cttttctatc catgtacacg
    23461 tttcatataa tcttgataac tttcggtatc catgagttgt ttagcgttct tgacgcgatc
    23521 tgctgttggt ggtttgactc cttcgaggga atatggaatt ccaagttcac gccacttgaa
    23581 ctcacccatg gtgtgataag gtagaatttc aaacttatca acatttttga gggtcttgac
    23641 gaacttacca agttcaatca agtcatcatc tctgtctgtc aatcctggaa ctagcacgtg
    23701 gcgaatccag acaggttttc caatatctga tagatactgg gcacaagcca agatattttt
    23761 attggtttgg ctagtgacaa tcttgtgctg ttcttcgttg atttccttga tatccaaaag
    23821 aaccaagtca gtgacagcca tgagtttgtc aaacttctca aggtaacgtg gtttattacg
    23881 gaaaggaaga gcacaagtgt ccaaggtaca gtggattcct tgttccttag ccttggtgaa
    23941 gagggcaatc aggaaatcaa tctgcaagag agcttctcct ccactgactg taatcccacc
    24001 cttatttccc cagaaaccac ggtagcgcaa ggcctctgtc aagacatcat ctaccgtccg
    24061 ttcacgtgac ttattggact ccatagccca agtgtctggg ttgtggcaat actggcaacg
    24121 catgtgacag ccctgcaaaa agacaataaa gcgaataccg ggcccatcta ctgacccaaa
    24181 gctttctgtc gaatgcacca ttcctgtcac ttgtccataa tcaattgttt cttcagacat
    24241 atcgttacct tccttgaaaa cgttttatag ttttattata tcacgacttg aaggaaaaac
    24301 aagatttttg cctatttttt agaaagcaat ctgtttttca atttcatttt caatctatta
    24361 tcagattatt cttcaaaatc gaagccatat aaggttgcaa aatagtcctc agggcgctct
    24421 gcacggcgga tttgacgggc tttaccttct tcggtataga ggatttccgc agaacgtaat
    24481 ttggcattat actggtagcc cattgaaaat ccgtgggcac ctgtatcatg aattaccagc
    24541 aaatcaccga tttctgtatg aggcagttcg cgattgactg caaatttatc attgttttca
    24601 cagagtgaac caaccacatc taccgcttca gctggtccat ctggatgggt cacgttgcta
    24661 atatgatggt aagctccgta catagctgga cgcatgaggt tgactgctga ggcatctaca
    24721 cctagatagg tacggtaggt ttccttctta tgagtgactc ttgtgactag agcaccgtga
    24781 ggtgccagca taaaacgacc caattcggtg aaaatcttga cctgaccaag acctgctgac
    24841 gtaagaactt cttcatacac cttacgaact ccctcaccaa tcaaggcgat atcgttcggc
    24901 tcctggtctg gacgataatt aacaccaata ccgccagaaa gattgataaa gtctaacgaa
    24961 atgcccaact tttccttgat ttcaacagcc agttcaaaga gctgacgagc caactctgga
    25021 taatagagat gggtcacggt attggacgct aggaaggagt gaagcccaaa agtcttggct
    25081 cctttttcct tcaagatagc aaaggcttca aagagctggt ccttggtcat gccaaactta
    25141 gcctccccag gattgtccat aatgtctgtc cccagttcaa aaacgcctcc aggattataa
    25201 cgacaagaga tgatttctgg aatgcctgct actctctcca gatgttcaat atcttcaaag
    25261 gcatccaagt taatggtcgc acccaattca cgtgcatagg cgtattcctt gtctggcgtg
    25321 ttgttggaag agaacataat ctcagaaccc agaaagtcca gtttatggct catcaaaagc
    25381 tctacataac tagagcagtc cacaccacaa ccttcttctt ggagaatttt caaaatagct
    25441 ggagttggag tagccttaac tgcaaaatat tccttaaagc ccttgttcca cgaaaaagct
    25501 tggttgacag ctcttgcctt ctcacgaatc cccttctcat catacaagtg aaagggagtc
    25561 gggaactcgg caacaatcgc ttctaactct tctctattga taaatggtgt tttcataagc
    25621 ttccttctat tctgtttgca aagaaaggct gggacaatcg ttccaacctt tagttctatc
    25681 tcttatttat cttcgtcaaa gatattgcca acctcaacat cgatggcttg gttcttgaca
    25741 tcaatattag ttaccttcaa gagtgacttg tagtcaaact gcttttcacg ttcttcttgg
    25801 gcattgtccg caaagaccgc tacacctgcc agttctgagt cgaactcgcg caagagacta
    25861 atcataccgt tgaccgttcc gccacctttc aagaagtcat ccacaatcaa gacacggctg
    25921 cctgccttaa gactacgttt tgaaaggaac attttctcga tacggtcacc acttgaacct
    25981 gaaacatagt tgacgctaac agttgaacct tcggtaattt tcaggtcacg gcgcacaatg
    26041 acaaaagaga cattgaggac attggcaact gcatttgcaa gtggcacacc cttagttgct
    26101 acggtcataa ccgcgtcaat tttttggtcc ataaagcttt tggcaataat acgaccaata
    26161 tttttcaaga tggctggtgt gctaagcaaa tcagacagat agatataacc acctggcaag
    26221 atacggtcac tttctgacaa cttggtacgc aagtcttcaa ccatttcctt agcatcctga
    26281 ctcgaaatag acggtgtgaa gatgacacct ccgccagccc cagtcactgt ctggatatga
    26341 ccgatttcaa tttcctcaaa ggcgcgtttg ataatgacga tatcttctga gatggatgat
    26401 ttagcagact catacttttc agcaaaagta ttgagactag ttagtttata aggattatta
    26461 atcaaatagt tggaaatgac aaccatccga tcacttcttc ttaatttcat ttctatttcc
    26521 tattccattt ttattcaaaa aatcaaaaag caaactagaa agctggtcgc tggtggttca
    26581 aaacactgtt ttgagattgt caatagaact gacaaaccct gtaatatacc tgcatatata
    26641 catacgacaa agcgatacta ccctagtttg aagagatttt cgaagagtat tcatttttgt
    26701 cttttactta ttataccata ttcacataaa aaaacgaaca ttcttatcct aaaaaatgct
    26761 catttttctt aaattatcaa tctaaatctg gtttatagaa ggaacgatta tccatagcga
    26821 agattttatt ggacatctct cctttatcca ccaaagccag agctgttgac atcatcatca
    26881 tgcttgcatc cagattgtca atcatatgga taatctctgc ctccataata cgtggacgga
    26941 ctggacttcc atattcaagc aagccgtggt gactgaggat gacatgacga agcaaaacga
    27001 cttcttcctt ggtatcatcg atgccgagtt ccataactgt cttggtaatt tcgctatcaa
    27061 tgagagcgat atgtccaaga agattacctc gcactgtgta ctctgtctgg tctggccccg
    27121 tcaactcgat aaccttagct aagtcatgca gcataatccc cgcatagagt aagctcttat
    27181 tgagctgagg ataaacttcg ctaatagcgt ctgccaaacg taccatggtc gccgtatgat
    27241 aagccaagcc cgtttcaaag gcatggtggt tggtcttggc agctggatag gagtagaatt
    27301 ccttatcata cttggtgtag agatttcgga caatccgttg ccagacagga ttttcaattt
    27361 tgaaaatcat ttgcgacatg tagtcacgaa tttccttgac atcaactggt gacttgacct
    27421 tgaaatcggc tgggtcattg ggttcaccag cttgaggcag gcggagagta atttgattga
    27481 cttgaggggt attgttataa acttctcggc gtcctttcat gtggacaacc ttacctgcgg
    27541 taaaggcctc aatgttatga ggttgggcat cccagagctt cccatcaatc tcgccactat
    27601 catcttggaa ggtaaaggct aggtagtttt tcccagctcg agtttgcctc aggtcagctg
    27661 atttgattag gtaaaagcct tcaaatagct catctttttt catgtgacta atcttcatat
    27721 tcttcctcat tttcttgaaa atggagtaga tcaagcgcag gctcgccttc tgacaactca
    27781 atgtgacgga gcgtccgctc gatagctatg gtacgacggt ttaataattc atcaatattg
    27841 ccagaggcat gttggagatg tttttgtgcc ttgaccagaa taccaccaaa cttgccaaac
    27901 tcggtcttga cactggcaag agtcttgctg atatggtcgg cactcttttg gatattaagg
    27961 gtcttgaaac caactgatag ggagttaaga agggctgata gggtacttgg tcctgcaacg
    28021 ataatctgtt cttcccgtct caaatcatca aagaagaccg gattgcggac gatttctgag
    28081 tagagacctt ctgtcggaac aaacaaaact ccaaaattgg tcgtccgagg tggtgctatg
    28141 tacttgttcc taatatccct agcaaaacgc ttgacacttg ctaggagtga cttacgacag
    28201 cgttcgattt catccttgtc acctacttca taggcttctt ctaagcggta ataatctgcc
    28261 agtggaaact tagagtcaat tggtaggtag acgtattctt ggtcgccttg tccgggtaac
    28321 ttgatggcat actccactcg ttcactagag ttttcaaccg ttgcgtattc tcgttcgtac
    28381 tgggcaggtg tcatgatgtc ttcaataatt tgccccagtt gcaattctcc cagaatccct
    28441 cgcgtcttgg ttccagagag aaccttgtta agagctccga catcacgggc aactgtctgc
    28501 atttctccaa gaccacgatt gacagactcc agttgtttag aaactgtctc aaaggaagcc
    28561 tgtaagcgtg tctgcaaggt cttttctagt ttttcctcga ccgtctggcg catttgttcc
    28621 aaacgttgct catttgattc ctgcaaggct tggagacgtt ggtctgtctt gtctctagtt
    28681 tggaggagat tatctgtcat ttcttgacgg acttgggtca ggccttggtg caattcaatc
    28741 cgcacttctt gcaaacggtc gctgacagcc acttccaaat ctttttggtc taactggctg
    28801 gcttgtctgg cttggtcaaa gcggtaatcc aactggtctg acaaatgatc tgcctgatcc
    28861 tccaagctct tacttaagtg tttctcctgc ctatcctgtc tttgccaaat cagaaagaga
    28921 ccagctaggt tggcaattaa taatagaata agtaaacttt ccatcctacc tcctgtcctt
    28981 gctatgcagt acgaccacat aaccatctgg gcaagtcacc gacacttccc tatctatata
    29041 ttcgttagaa gcgtacactt ttttaaagaa aaaattctcc tctgtcaact catacttagc
    29101 tccaagaata gtcagctggc tatcccgaac tggcataaag gctagatagt cgtagtctga
    29161 acgaggttct agctgactgg tcccttctga acaataggca atcaaatttt gcccatcctc
    29221 aatttctatt tgacgcatat agggtgccaa cttaggattg ctaggtagaa agacattggc
    29281 caacatatgg tcaatacggc cacccaaggc accgaaaata gtgacctcag cctgaggatt
    29341 ttgttcaaag atggttaaga gagccaattc cagatctgta tcatcctttt ctggtcgggc
    29401 ttggacaaaa tgctgggcac gtttttgaat cacctgtcgc tcttcttccg tcacagaatc
    29461 aaaatctccg actgctagag caagaggtaa gtcttcttcc aagacccaga gcgagcctcg
    29521 atccacccca acaaaagcat caaaatctgt ccgataatga ccgcggtttc cgcctgcaaa
    29581 aacggcaacc ctagtccagt tgttttctga gagtttgtac tcgctcattg acttctccct
    29641 taaagacata ggaacctgct acaaaaacag tcgcaccagc ttctttggct tgagcaatag
    29701 tttggtcatc aatcccacca tccacttcga tttcaaagtt caaacctttt tcctcacgaa
    29761 gagcaaccaa ctcacgaacc ttatccatgg tttctggcag aaaggcttgc ccaccaaaac
    29821 ctggattaac cgtcatgact aaaacttggt caactagatg aaggacgtgc ttgatggctt
    29881 caactggtgt gccaggattg ataacgactg aaggcttaac tccgagtaaa cgaatttttt
    29941 ggagggcgcc atgaatatga ggtgttgctt ctacatggat actgatgatg tctgcacctg
    30001 cacgcgcaaa atcttccaga tgatgctcag ggtttgacac catcaagtgg caatcgaaaa
    30061 ccatcttact atgaggacga aggctctcga ccacacctgc accaaaactg atttgcggta
    30121 caaaatgact gtccatgata tcgatatggg catattctgc cccagttgct tctagacgtt
    30181 tgatttcacg ttcaaagttg gcataatctg ctgccagaat tgacggagca atcttgtatt
    30241 gagacatagg tttctcctta ttttggaatt tttttgctga cttttttata ggtttctcta
    30301 cgattttcaa tttcactaag gaattgcagg taattatcaa aacggaaggt tgcaataaca
    30361 ccctcttcaa cagctggttt gacggcacaa gacggctcat gggtatgggt acaagtacgg
    30421 aacttacaat ctcggctaac agtagcaatc tctgggaaag cctgattgag gtcttcagcc
    30481 cttgatactt catagtccag agatgaaaag cctggtgtat ctgcgatttt acccccgttg
    30541 agattgtaaa aactaacagc tcgagtggta tggcgaccgc gacctagact gtctgaaatt
    30601 tctcccgttt caagattgag gtctggtacg agtttattga gaagagttga cttcccaaca
    30661 cctgtctgcc ccataaagac cgtaaccttg cctgttaaca aagacaggag ttcctcttta
    30721 ctggtcacaa agtcatagcc gatgtcacca taggtctgcc ggtaaaaatc cagttctccc
    30781 ctatcttcca acaaatccat tttggaaata tagacaatgg gatggatgcc cttgtgctcc
    30841 aaaagaacca agaaacgatc cagcaaattg ctgttaaaat caggttcctt gacggacatg
    30901 attactacag cttgatcgat attgacaata ggcggacgga ccagactgtt tttccgttcg
    30961 tgaattttga ggatatagcc ttctgaattt tcctcggcag agaaatctac ccagtcccca
    31021 acataagggg tatggccttt tttacggaaa ttcccacgcg cgcgtgtttg ataaacctgg
    31081 ccatcactct ccacatagta gaaacctgcc aaggctttaa tgatttgtcc ctgcatctta
    31141 gagtccttgc cctttaagtg cgtctgctag accggcaaat tctgccaagc tgagagcttc
    31201 cccacgcaca cttggtgaca agcctgcctg gtccaaagcc ttggtcagct tgtccttgac
    31261 ctcttcagtc ttaccaaagt aacctgtcaa gttattccac aaggtcttgc ggcgatgggt
    31321 aaaactagcc ttggaaacct taaagaaaaa gttctcatct tctactgcta cggctggctc
    31381 tggacgacgc accattttca agatggctga atccacattt ggcgctggca caaagaccgt
    31441 acgaggcacg ataaaggcaa ccttggctgt catgtaatac tgcacggcga tagacaagct
    31501 accgtaagcc ttggtgttag gctgggctga aatgcggtcc gctacttctt tctgcatcat
    31561 gaccacaaac tcacaaaaag gaatgccact ctcaatcaag tgcatgagaa taggcgtcgt
    31621 gatgtagtaa ggcaaattag ccactacctt gattggcagg ttaggatttt taaaattttg
    31681 gatatgttgc gccaaatcaa ccttgagaat atcttcgtta actacagtca cattatcaaa
    31741 atcacgcagg gtatctacca aaattggcac caaacggtgg tcaatctcaa aagccatgac
    31801 ttgggctgca cgctcagcca gaaactccgt caaggcacca atacctggcc cgatttcgat
    31861 gacattgacc tggtcatcaa tttcagccgt atccacaatt ttttgaagga tattggtatc
    31921 cgtcaggaaa ttttgcccga aggacttttt aaaggtaaaa ccgtgacgct ccagcactgc
    31981 cttggtcacg ctataatctg caattctcat ctattctctt ttctattatc tgttactact
    32041 attgtaacac attccccttg tattttggga atgaaaatca taccaaaaaa ctatctttcc
    32101 tcatgaagaa agatagtctt ggaaatcttt aaaagtggcc ttaacagttc tctcgaaaca
    32161 tgcgttcaaa aaaatcagct tcttgataac ttcgattttc aggtagggtc aaatccacat
    32221 cctcctccac atcatccact gtcagttcaa ccgtctgagg taaatttttc aaacgataat
    32281 gcttttgcct ttgcccttca atactagcaa aatcaagcaa atagccatgc actcggattt
    32341 gaaccaaaag gagatgattg gtcatatttt ccatgatgag gatatcttct aaaccacctc
    32401 tggcactagg ataatcagat accctgcgaa tacggatttt ttcacacatg agcaagtttc
    32461 ctcatatcaa tgatttcatc agccttattc cagactaggg gattatgggt cgcaatgatg
    32521 ataattcgat tttcatcttt caaatccacc aagagattca taacctcctc tgaattttca
    32581 ggatcaagag ctgctgttgg ttcatctgcc aaaatcaagg gtggattttt caaaatagtc
    32641 ttagcaaggg caactcgttg ggcctctccc ccagataaag tatagatttt ttgttctaaa
    32701 tccaaatacc ctagattaac tttttctaaa gcccccactt gcctttccaa acgttctact
    32761 tttgagattt tctgaccaac aaaacccaaa tccaaatttt ctttgattga ttggttttct
    32821 aagaggccga aattttgaaa gagatagccc atctggtctc gaaaatactc acgagtggga
    32881 atggttttta aatctttccc ctgatagaga acccttccac catctatctt ttctagcttt
    32941 cccaagatat tcagcagcgt cgtctttccg cttccactct ttccgattaa ggcataaacc
    33001 ttgcccttct ccagcttgag attcaaacca gagaaaatcg cgcggtcatt aaatttcttt
    33061 tccaatcctt gaatatcaat catcttattt tcctttcaat actgccatgc taccagcttc
    33121 ttcttttttg tcctgcctta ctaaaatcaa gagggcgtta agcgtaaaca aagctacaac
    33181 tagagcgcta atcaaaccat ctcttgtcaa aatactagat aggactaagc caagcaagag
    33241 aactcctcct tgcgccagta aatacttgcc atgaagctca tagattgtca taccagcaag
    33301 acgtttaatc ataagctccc gtctgaactg ctcaaaatag agaagattca tggaatcaaa
    33361 taacaagata gccgtagaca gggtcaaaat cgtcccaata aggagagagt aaaactccac
    33421 ccgtttgtca tttagtacct tggcaaaaaa tagctggctt tttaccaagt aagaaacctt
    33481 gtgatacaga ccttgctctt ttagagctgt gatgctgtct ccatatcgat ctagtttcaa
    33541 ggcactatta gccgtagttc cccacaacat ccctgcaaca ggtcttgttc cagtcgcttg
    33601 cggcgttaga actacaataa tcggatcttt taggtactgg cgtgttgtct tgtacccatc
    33661 attatagagg aaacgttcct gtcctgtttc tgtaaaagct agccttacct gtgggaggta
    33721 gtgtttctga ctcgtcactt ctacactttc agatgaaaaa ttttgcaggt aatctgtaaa
    33781 caatccttgg tagtagacag actgctctcg caagctctca ggcaagatca gcccaaactc
    33841 tccctcagac aagttttgca tcttgtccat aaactctgaa gaaacggtaa tcttttcttc
    33901 tatcagatag cgtggtgaga catagataac attccctgac ggtgtgtagt cactgagacg
    33961 attgccatct aggtccactt ctgctccatc tgagaaattg tcaacattgc tcataatata
    34021 aaaagagtct gtattggcta accgttcttc agtaaatgtc tgccactcac gattatcctt
    34081 acgcgttcct tcttcatcgg caaatgcact agaccaacca aaggatagac ggtaacggtc
    34141 tgaggactgg ctccatttat tgctagctct ctccatttcc tgcatttcac ggtagtgggg
    34201 taggagagct gtcgcactcg atccgaccac caatacagct aagagttgcc ccaccatcat
    34261 caatgtcatc atacgtttga gagggagttt ccctttcaat agatccacca gactattttc
    34321 ctgtaaacca agtagatgga cgacacttag taaggtagaa atccctgcca aggtcaatcc
    34381 atagagtaga agagcaatga tgaccagttg caccgttgcc ataaacaagg caccttgata
    34441 ccagagaatc cccaatccca aaagactgga taccagcact gagcagataa gctgtctcac
    34501 atcttctaac actggtctga gagcaactcc aaacaagctc tcaccagcta ttaagcgaat
    34561 ccctgcttga cgaagggatt ttatccgata aatcagggtc agactcataa aggtcagcag
    34621 aaaaatagct aaactcagta gcaccatagg ggtggccgtc aatagtaaga ctatcgaaaa
    34681 tggatcttct ccattcgaaa caaagccttg atagccaagt tctttcaagg tggtctgtaa
    34741 gctcactcca tccaaacttc ctgacacaat caagtaagac cctaataaat cactcgtttc
    34801 tgcactctcc ttggaggaaa tggtcagacc ttctggaagc tctccctcac cataagttgc
    34861 ataggtaaag cgtgtttctc cagcttcatt tggctcaacg attcgacggg cgatgagact
    34921 attatgctct accgccaagt gactcaaagc ctctgtcacc tcttcgcgac tagctactgg
    34981 ttgatttgtc atcattcctg tcacatcaac agaaggatag ttaccaaata aaatctcttc
    35041 tcgatggtca acggaagtta tcaccatata aagcgatacc aataccattg aaatcaaaat
    35101 aaataaacgt ttcatcattt cctccaaaaa gttacttggt cacttagtga gaaggtgacc
    35161 aagtaaagct aaacaatagg gctataccat tctaaacatt atagtagaat gcaactttct
    35221 ctccccaaga agtacgaatc catgcacgag aagtccctcc agcaccagca tatcccttgt
    35281 cagatgaacc agtttttctg ctaacaactg ttgaactatg gtattttgaa gggtgatagt
    35341 agtttgagta agcaccaccg ttacctgaac cataagtcca aacaccacct tctgggtatt
    35401 gaacggctgc agaggcaact gctacacttc cacctaatgt tgctgcaatg ctcgctacaa
    35461 gagctaattt tttaactgtt tgtttcatat tgatactcct ctatttttga ttacaagatt
    35521 gttgagttac ttgctcctct ttccattatc aacgttgata ctattatatt accaaatgat
    35581 attctaaaat tatttgtttt tctctaaatt gtcaaaatag caccctgaaa tgaactttta
    35641 tagtcattat actccatctt tatcacacta tcaaggacat caatgtcctt aaaatctaac
    35701 tatttcatat tatttttagc ctatatctaa aaaaacagtt ctaaagacga actttagaac
    35761 tgttttgata cgaggacaag gacatttttg tcactgattc actatgttta taaatatttt
    35821 ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaattcatc tccaattagc
    35881 tgcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc tctttcttct
    35941 cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata atcttgagtt
    36001 ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt tactcctacc
    36061 ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagaa gttttctaat
    36121 ttagttacct ttttctgttc caaaagaaag agcattagct tttcgatttg atttataaat
    36181 ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa caacatatca
    36241 tttgttgaat aaacttcttt ttctatgatc tgagacatat gttcctcaag tattaactct
    36301 gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac ttcacatgct
    36361 aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc caaacacgct
    36421 tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat ctggtatttc
    36481 aattctaaat aagcaggagg aagagctgaa aaatccggca taaggctata aactggaact
    36541 cctagccggc gagcaatata ggctaacttg ggcaaactcg gttgcgaagc tccactttca
    36601 attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaatc tggacgacta
    36661 actccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag acgcctcctc
    36721 ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggtca catctttcct
    36781 actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac aactctaggc
    36841 gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg agatattccc
    36901 tacgcttacg gctgtctgcc cctactagaa aaccaaggcg aatcaagtcg ctacgactaa
    36961 tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc gttttcaggt
    37021 cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac ttgggaacag
    37081 cttcatctcg cttgagaaag gcatgctgaa ctgttggaat gaccgtcatg atcatgcgcc
    37141 gaatccgttc cccattaaaa tctgggtctg taaagacaat gactccatga cgttggtgca
    37201 ggcgctgaat ccgctctata tcctgatcat tgatggcaga acctcgagtc tcataggtct
    37261 ccacatcgaa ataacgtttg agattgaccg tatcatcacg cccttcaacc acaattactt
    37321 gggaaatttt ctctttcatt acttgctgtc cagtccaaaa attcgttctg catttgcagt
    37381 cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa agtcgaccac
    37441 atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg ctaagtaagg
    37501 cgcatctgtt tccaccaaca tcttgtccaa aggtaactct ttagctgctt cttggaggtc
    37561 agttgccttc ttaaaagtca ccactcctga gaaggaaatg gtcataccaa gatccacaaa
    37621 cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac gaggaccaac
    37681 gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat ggacaacaaa
    37741 aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct cctgcacctc
    37801 tttgggcgct gtcatccaat ggtaatctaa gccaatttca cctaaagcca caaccttggg
    37861 atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac cagcttctgt
    37921 aggatgccaa ccaatagtcg catagagctg ctcatactca tctaccaact ccaaggcacg
    37981 ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca tctcagcagc
    38041 caaggcaatt tcttctgcct cacgacctgc aaattcttct acattcaagt gtgtatgtgt
    38101 atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa aaagaggagg
    38161 ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct gctatctttt
    38221 ctatccgata ttgcccatct cctattccac agttagagac agaagagatt ggctacattg
    38281 atagactatt cctattcagc taaagtatgt gattatcttg ggttctcact tttctcaccc
    38341 atcactagga ttgatgactt ctcaaaatcc cccttataat ctaactttaa gtagagaata
    38401 gaattttcta gctgttccat tttctacgtc tagtctttct tcctagcaaa gcaaaaccac
    38461 tggttgccgc cactagacca agactagcaa gtgctgcact tgatgctgaa cctgtgttag
    38521 gtaattgttt gccagcaggt gactggtagg taacatcttg cattgttcct gcagttgctt
    38581 gcggtgcact tgttgtctta ggagcagcat taggagccac tggtctctta agaactgtaa
    38641 ccatggcaat ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc atagttttcg
    38701 tattttcaat ctctttgaga gctgcttgtt tttctgcttc cactcttgct aaaagttctg
    38761 ctttttcttt atcagaaagc ggtgcgcctt tgatttcatc ctgcttgtcc ttggctgctt
    38821 tttcaatggc atttttagct gattcttttt cagtagctaa ttgcttagaa gcttgtgatt
    38881 gagctgagtt ttgtgaacct gtgtgagctg gtccacctgc ctggctacca ccgcctgcat
    38941 tcgcattagc atctgagctt ccagtattgc ctcccttatc tacacctagt gtagtttctg
    39001 gcttatactc tggtgtagct tcttgagtta cagattcacc tttcgcagat tgttgaactg
    39061 tatcttttcc tagaatcgtc actactgaac catctgggaa tgtgattgtt gctgtaccat
    39121 ctccagctac ttcaacttta tagcaaaatg aaacaataac aggacaaatc gatcaggaca
    39181 gtcaaatcga tttctaacaa tgttttagaa acagaggtgt actattctag tttcaatcta
    39241 ctataacatc gcttccgaat ccatttgcat ctactgaact tttttacaaa aataaaaatc
    39301 agtagactcg agttcctact gatttttgat agaattcgtt tggattatgc agccaaaact
    39361 ttgcgtcctt tacgacgacg agctgccaat acgcgacgac catttttagt tgacatacgg
    39421 ttacggaatc cgtgtttgcg cgcacgacga agtttacttg gttgataagt acgtttcacg
    39481 atgaatacct cctcatagat tttgtattcg tttagccggc tataaagtga tcagttacta
    39541 aacatacttt actattctat ctgattcttt cacttttgtc aatagctcta ggcaaatgtt
    39601 tccagctttt tcgaatgcaa gccctatcta cgatactgtt tcaagaaacg agtcatcttt
    39661 tcttggattt gggctaactc atcaacacga ggaaggtaaa cgatacggaa gtggtctggt
    39721 tcctgccagt taaagcctcg accatgaacc aagagaacct tttcctgctt caagaaatca
    39781 aggacaaact gctcatcatc atcgatacgg tacatattgc ggtcgatttt tgggaagata
    39841 tagagccccg ccttgggttt aacggcagac aaacctggaa tatcttgaat ggcattatag
    39901 atgaaatttc tttgctcgta gattcgtcca ccaggaagaa gcaattcatc gactgattgg
    39961 tgacccccca aggaagtttg tacgacttgt tgggccaaaa cgttagagca aaggcgcata
    40021 ttggacagca tattgagccc ttcgatatag cccttaacat gagtcttagg gccagacaag
    40081 accatccaac ccacacggaa acctgctatg cggtgggatt ttgacagacc attcatgctg
    40141 acacagaaga catctggtgc caagctcgcc acaggcgtat gcacatgtcc gtccattacc
    40201 atgcggtcat agatttcatc cgcaaagata atcaaatcgt tttgacgggc aatctcaata
    40261 atctccagca agagttcctt aggataaagg gctccagttg ggttatttgg attgataagg
    40321 acaattgcct tggtattgga agtgattttt gacttgatat cgtcaatatc tgggtaccat
    40381 tccacagctt catcacagat atagtgaacg gcatttcctc cagctaggct gacagccgcc
    40441 gtccatagag gatagtctgg cattggcact aagacctcat ccccattgtc caaaagcccc
    40501 tgcatggaca tgacaatcaa ctcactgaca ccatttccaa ggtaaatatc atcaatatct
    40561 acgtttggga atttcttcag ctggcaatac tgcatgatgg ccttacgggc tgagaaaatc
    40621 cctttggagt cagaatagcc ttcactatca cgcgcattca taatcaagtc atgaatgacc
    40681 tcgtctggcg ctgtaaagcc aaattctgct ggatttcctg tattcagacg taaaatcttt
    40741 tctccattcg ctcgcatccg catggcttct tccaatacag gaccacggat atcataagca
    40801 acatgctcta acttactaga cttgttatat tctttcatct tgtttcctca attcatcagg
    40861 atagtaacat tataccacta gaaagagaat gcgacaagtt tactgaaatg tgttactagt
    40921 ttcgtataga agaaaaacct tgctaaaggc gaggttaaga acgtagagat agcaggtatt
    40981 ttgcaaagac acatcacata ccaaaagata aacctagaaa aagcaagcct agaaaggagt
    41041 cataaagaag attaaacatg aggaaaagcc cccatatcat caagtaatcc caacgcccac
    41101 ttcgttttgc aactagaaat aagattagat ggtagagtac atgaaaaact aaaaatccaa
    41161 taaaacttgg ataaagaagc ggtaccagac tctctaattg acagatcatg atcacttcta
    41221 ccaagactga aactaggata attccataat aaaggaaatt tgatttttga aaagagtttt
    41281 tcatcattct cccttccttc acttctttcc tgctatcctt tcttttattt tatcatatat
    41341 ttatatagag tttaagccct aatattcttt tgaaaaatac tttttttgct ttacttctat
    41401 agtgaaaagg tttacaatta tagtaagaaa atttcaggag gtttcattat ggcacaacgt
    41461 taccaaaata tcatggtcgc aatcgatggt tctaaggaag cggacttggc ttttgtcaag
    41521 ggagttcatt ctgctctacg aaacgacgct aaactcacca tcgcacatgt cattgacaca
    41581 cgcgctctcc aaagcgtatc cacctttgat gctgaagttt acgaagaact ccaagtcgac
    41641 gctgaaagtc tgatgaaaga gtacgaaaaa cgtgctaaag atgctggggt agcagatgtt
    41701 catatcgtca ttgaaatggg aaatccaaag accctgctag cacgtactat tccagatgcc
    41761 gaggaagtgg acctcatcct cgttggcgca actggtctca acgcctttga acgcctcttg
    41821 gtcggctctt catctgaata catactccgc catgctaagg tcgatttgct ggttgtgaga
    41881 gaacaagaaa aaaccttata atcacaaaga aaaggagccc ctagctcctt tttgtttacg
    41941 atttatttct ctctttatgg cgttcgtaag ccttgagctg gcgctgcagt tcctttttaa
    42001 tagcaggttc tggagcatat ttttcttccc aattatctgg ttttaagatt ttatgggtca
    42061 ctggatcaaa atgagccttg ccatctggaa aaattttccc catattggcc tgatggacaa
    42121 tatcaaaaat acgttctggg tccaccccca tcaagacaaa actgccgtag gtgaagtaaa
    42181 gcgtgtcaat caaggcatcc acttgcccta tcaaatcttg ctgagcaggt gtcttcttgg
    42241 ctactttatc tgctgcctta tcaagggcct gatgaagttg cgatacagct tgaccaaaat
    42301 cttcttcaga aggactggct gctcgaacaa actccaccaa ttcttctatt ttaaaaccag
    42361 ccctatgggt tgcaccctct aaatcccaag ctcgaggttc ttcttgggtt cgttcatcca
    42421 tcatgtggtg gaaggtcttg accttattga aatgatagtc acggctgaca aagacttttt
    42481 ctgaagacaa aacaccaaaa tgttccaagg ccttgtggat cccatcttgt tgattgctag
    42541 tggtaatgtg cttggcagct tccttgacac tgctactacc atttcccata gcgaccgaca
    42601 taccgacgcc agccagcatt tcaaggtcat tatctgagtc accaaaagcc atgacttgat
    42661 tgaggtcaaa gccatattct ttcccaactc ggcgaatgcc ttctaattta gaatttccct
    42721 gattgatgac atccgatgca aaaggattgc tacgtgtcaa tttcaagtct tcaaaatcag
    42781 ctgccgcctt ctcagattct tctggtgtca tcagcatcaa aacttggtag ataggctgat
    42841 tcatcaggtg aagcaggtcc tcttcctttt ggggaacaac cttgctgacc atgcgattaa
    42901 aagactgact caccgtccga gttaaaacag agggaacgaa gcgactaatt cgttgggaaa
    42961 aagaacccag accaaaggac atgattttag aacccaacat ggcatccttg gtccctaggg
    43021 caatctccgt gccctctttt ttagcatagc taattagatg gcgcaaatgt aacttggaaa
    43081 tagggctcgt gaacaagact ctgtctttac taaagatata ctggccatta taggttaccg
    43141 caaaatccag atccaaatcg tccatcaatt ccttaacaaa aaaaggtcct cgccctgtcg
    43201 ctacgccaac tagtacccct tgttctttga caatcttaat cgcatcctta gtggatttca
    43261 aaacactctt gcgattgttg accaaggttc catcgatatc aaaaaaaaca gctttgactt
    43321 ccatcctatc ccaatctccc cttttgtgat acaatgatta taccacattt cagaaagagt
    43381 tagtaaatca tgcctaagaa aatccttgtt ttacatacgg gtggaactat ttccatgcag
    43441 gccgatgctt ctggcgctgt tgtgacgagt tcagataatc ccatgaacca tgtgtccaac
    43501 ccacttgaag gaatccaagt ccacgccttg gactttttta accttccaag tccccatatc
    43561 aaacccaaac atatgctggt cctctaccag aaaattaaag aggaagcaga taactacgat
    43621 ggagtggtga tcacacacgg aaccgatact ttagaggaaa cagcctattt ccttgatacc
    43681 atggaagttc cccatatgcc tatcgttcta acaggagcca tgcgtagctc caatgagctc
    43741 ggtagtgatg gtgtttataa ttacctaagt gctttacgag tggccagtga tgacagggct
    43801 gctgacaaag gagttttggt cgttatgaac gatgaaatcc acgctgccaa gtatgtcacc
    43861 aaaacacata cgactaatgt caacaccttc cagactccaa cacatggccc cctcggtctc
    43921 atcatgaaac aggaaatcct ctacttcaaa acagctgaac ctcgtgttcg ctttgacctt
    43981 gatcacatac aaggtttagt ccctatcatc tcggcttatg ctggtatgac agatgagctg
    44041 attgatatgc tggatttaga acacttggac ggtttgatta tccaagcctt cggagctggt
    44101 aatattccca aagaaacggc tcaaaaatta gaaagccttc tgcaaaaagg aatcccagtc
    44161 gctctggtat cacgatgctt taacggtatt gccgagcctg tttatgccta ccagggtggg
    44221 ggcgtacagt tgcaaaaagc aggcgttttc tttgttaaag aactcaacgc ccaaaaagcc
    44281 cgcttgaaac tcctcatcgc cctcaatgcc ggactaacag gacaggcttt gaaagactat
    44341 atggaaggct aatactcttc gaaaatctct gcaaaccacg tcacgtcgcc ttaccgtatg
    44401 tatggttact gacttcgtca gttctatcta caacctcaaa acagtgtgtt gcgcagcctt
    44461 taaccagccg cctagtccgc tctatggtat tcattaagtc aacatctctt gtttaagagc
    44521 acaaaatcag gaaatcttct cgattccctg attttttcta tttacgtttt cgtgttgagc
    44581 tacgttctgt caaaccatga ggtaagagaa cttcacgttc ttccaactct tccttatgca
    44641 taatcttggt caacatacgc atactaatgg caccaaggtc ataaagaggt tgggcaatcg
    44701 ttgtcaagtt tggacgggta aagcgtgaga tttgtgaatc atcactagta ataatttcaa
    44761 aatcttctgg cacagaaaca cccttatcag ccaaaccgtt caagactcct gctgccaact
    44821 catcacctgt cacaactgct gcagttgcat ttgatgaaat caaacgctct gctaaggcgt
    44881 aaccatcatc atagctatat ttagattcaa ataccaaacc ctcactataa gcgattcctg
    44941 cttttttcaa ggtttccttg tagccaacta aacgaacctt accattgatg tcatccacta
    45001 gcggaccgct aacgaaagca atacgctcat tttctttagc aaggtaactc actgcatcaa
    45061 ttgttgcttg cttatagtca atattgacac ttggcaactg gtgctcaaca tcgacagttc
    45121 ctgcgagaac aatcggagta cgtgaacgcg aaaattctga gcgaatttta tctgtcaagt
    45181 gataacccat atagataatg ccatctacct gctttgaaaa gagggtattg acaacagaaa
    45241 cttctttctc gttatcttca tcgctattag ctaggacaat attgtacttg tacatttctg
    45301 caatatcatc aatcccctta gccaaactcg aaaaataacc attggtaata tttggaatca
    45361 cgacaccgac agtggttgtc tttttacttg caagaccacg cgcaactgca tttggacgat
    45421 aatccaagcg atcaattacc tctagcactt ttttacgggt attctctttt acatttttat
    45481 tgccattgac cacacggctg accgtcgcca tggaaacacc tgcttcacga gcgacatcat
    45541 aaatggttac tgtatcatct gcattcattc cttttcctgt cctttctatc tcacacattc
    45601 ttttacaagt agaggtactg attgaagctc tatatctact tacaaaagtg aagatgtgaa
    45661 aatttcgttt tcatatttct acttattcca ttctatcact aattgtaaac actttcaagt
    45721 gttttttgaa gattgattga aaaaatttca tagaaaacct aggtttagct ccttgctacc
    45781 accttagact aaacaaaaag gaggaaacta agccctccta aagttatagt aaaatgaaat
    45841 aagaacagga taaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtag
    45901 aggtgtacta ttctagtttc aatctactat aggtattgtt ccattcacta ccgtcaattt
    45961 tagcacatag tcttcatgaa aatattatat catcataacc aaccagattc ttttgcgata
    46021 ttagctgcct ctgttcgatt accagcatcc aattttgaaa gaatattggt gacatagttt
    46081 cgaatagttc cgtttgagag gtaaagctga tctgcgattt cttggttgga taagccccgg
    46141 gcgatcccct ttaagacagc aatctcttgt tctgtcaacg gattgggacg ggtcatcacc
    46201 acttccatca attcaggcga atactccttg cgtccttcga ggacggtgtg caaggtttgc
    46261 atgaggtctg caacgcttcg ttcctttaat acataagcat ccactccagc cttgaccgca
    46321 cgttcaaaat acccagcacg cttgaaggtc gtcaccacaa ccacctttgt ttcaagcctt
    46381 tctgatcgta tccactccaa gacttcaaga cctgtcttaa caggcatttc tacgtcaagg
    46441 atggcgatat ctacagactc cttttctaat agttggattg cttcttgccc attcttggct
    46501 tgaaggacag actccacatc tggttgaagc gtgagcaatt gacacatggc atctcgcaac
    46561 atactttgat cttctgcgac taatactttc atcttagttt ctctccttat aaggtagtcg
    46621 aacttgcact tccgttggat gtttctgact gattacactt acttctcctg aaaatggaaa
    46681 aacacgatct cggattgtat ggagctcatc tccttttaga aaagcaaagc cgcagccatc
    46741 atcacttact gttaaaatga gttccttctc tgtccgttct aattttaagt aagctttaga
    46801 cgctttggca tgtttgatga tattggtcac caactcaagc aaaatcatag aagccattga
    46861 ctccaattct tgagtaaggc tagcagtatc tagttggtta tccgtttcca cctcaattcc
    46921 agcaatttct aacatctttt tcacagtctc tagttcggat gtcaaagttc tagacttaag
    46981 attttccaca atggttcgaa cttcacgcat tgattctttg ctaatttgct gtatttctct
    47041 taattccttt tccacttgtg gataagcctg catttgaaat agctgcaagg ctagatctgt
    47101 cttgacacta agcatagcaa aggtatgtcc cagactatca tgcaaatcct gaccgatacg
    47161 attgcgctca ttttctgcta gcaatagatt tatctgggta ttttgcttga cctgagcttc
    47221 tttcaaatcc tcgacaaggc gaattcgaac cgagcccaaa gtcatcaaat cgacaaaagt
    47281 aagaattaca agcatataga ctagaaactc aacttcgatt ctctgaaaaa tcaaaagtcg
    47341 ccccacaaca aggacttgag caaggagaaa tgtccataca tgtaaagact ttaaactacg
    47401 tacgccaaaa tgataaatta agagattgga aaggaaaaag aaaaaccaga tataattccc
    47461 agcaacaaag gcggtattcc cagctacata gaccagcatg atcccccaga aaagccatga
    47521 tatgcgctgg ttcttagtta ttaaaacacc caaatacgtc actacaaata gaatatcaac
    47581 caataaatgc caagcaggaa gctccccagt cactacaggt aggatgggga aaatcataaa
    47641 aattaaactt gcccaaaaca tatagtgtat gcttttcagt ctttcaagca ttaagcattc
    47701 tccttatgac cttgaaggta aatggtcaaa ccaaacaaaa ctgctgaaaa aacaagtaga
    47761 taaactgtgg ctgatagatt gatgccaccc tcgtttaaga aggtcttgag caactccatc
    47821 aattgatagg tcgggagaca cttaccaact gcttgcatcc agtctggaaa taaagaaatg
    47881 ggcatccaga gtccgcctaa aacagccaat cctagataaa gaagattgcc cacaacagac
    47941 atcagctgac tagttggtaa gagagtcaag gtcaagccaa gtgctacaaa agcaatactc
    48001 cccacaatca gcaaaatcgc agctccaacc cattttacta gaggcatgtc tacacctctt
    48061 acaaaatgtc caactgtaaa aacaaccaga attgagacca aataatcaac cagcatactt
    48121 gttatctttg atagataata ttctaccata tttacagggc tatgacgcaa tgttttctgc
    48181 cagttgttca tcttatcggt atgtaaaaca gctggaaatg agaggatagt tgtcgacatc
    48241 atggaaaagg cagtcatgga gatgaggtaa tcacgcataa aattcgcgag ttcacctggt
    48301 gtgtcctgat agataccaga aaaaaataaa tagaaagccg tcggcatccc taccgacaat
    48361 agataataga ttaattgtcg tttggtcaat aaaaattcta tcttactaag tgctatccat
    48421 cgtttcatct tagttatctc ccttctgcgt ctcttcaaag attgtattca acaaactacg
    48481 attattaact tcgatttctt gcatcctaca tcctgcttgg gctaatagtt cccagaaagc
    48541 atctgcttct cgagtgacta cttgtaaaga atcttgcttt agggtccagt tttcaaccaa
    48601 atttgactgc tctacgactt ccttgtaagc tataggaagg ataaagtgct tttctatttc
    48661 ctcgctacgc atagctagag gcgtcgtatc tcgaatcaac tctcccttat ttaagagcaa
    48721 aatgcggtct gcagtgtgtt ccacttcctc aatataatgg gacgaataga gaatggtgac
    48781 tccttgcgct tttaactcct ggacaatttc ccaaaaacgt tgacgagttg aggtatccat
    48841 ggacgcagta ggctcatcta aaaaaacaat ctttggtcgc ccaattaagg tcaagacgaa
    48901 agaaaagaga cgcttttgac cgcctgataa tttttctgcc aactgttctt tttgttgctt
    48961 gacaaactgc aatagttgat caatttcctg attgctcaaa ggatttggat agatacgttg
    49021 aaagaaagca atcaactctt taacttttaa tttctgaacg attacatttt cttgagggag
    49081 gtaagcccta gtatagtcta acttagaact cgtcactggt aaaccttgaa tagatacttg
    49141 accgcttgtg accagtttat ctccaagcaa acaatctaag agtgttgtct ttccggcacc
    49201 atttggacca attaaggcaa cacattcacc atcgttgatt tcaaaggaga tattccgcaa
    49261 aatttccttg tcttttattt tcttactcaa tttctcaact ttaaccaccg tcatgagact
    49321 ctcctttcaa ccactccatt cccataagga aaacgacgaa aatcataaat ccaaacccca
    49381 aagcaccacg aatgaattgg cgaagcaagg cttgatcaaa ccaatcagta aacatttcca
    49441 ctaaccatac caagagtgac aggccgataa agaaatagat gatccctctc ttcattcctc
    49501 aaactccttt ttcacatctc tgactaattt caaaccttct ctaacaagcc aagacatgat
    49561 tccaaagcca gcaaagacct tccaaggaaa atggtagaaa tcctcatcca atcccgaaaa
    49621 catgagatag gtcataactc ctgctactac taaacttact gcgataatca ttttatttct
    49681 catctcttct tcccccattt catactacaa ttatagtctt ttaaaatcag aggagacaga
    49741 agcttctgtc actaaaaaat atgacaaatg tcataaaaaa ttctgttcaa aacaagcaag
    49801 atacactata caataaaaca caatcagaaa aatctaaggc aacttcctca aaagagatat
    49861 caaacccaat tcacaccata atgtaaacta atacttattt aaaatcaaaa agagtaaaaa
    49921 tttttatcag acaaacacat atatagtgta ttgaatctat aacagtaggc cttaaatact
    49981 aaaatatttc tataaattaa tttaactttc ctgatagaac tgttcatatc ttatttcaat
    50041 tctctatatt atacgttgaa ccaaaccctt ctattgcttt cttaaaaatt tataagagtt
    50101 ataaaatctg ttaaatttca atgtgtatat ctaaactacg gtatttattg aaaagactgg
    50161 agacaaaaag tatacgctgc caaaatgaat tactgaaaat caaaaaagag agaaccaaac
    50221 tgattccctc ttaatgtata taatatctag ttttaaaaat acacactcac atatctctgt
//
LOCUS       DAVKWS010000007        48027 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKWS010000007 DAVKWS010000000
VERSION     DAVKWS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.007"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            312..2291
                     /locus_tag="VW852_000467"
     CDS             312..2291
                     /locus_tag="VW852_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271658.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2358..3854
                     /locus_tag="VW852_000468"
     CDS             2358..3854
                     /locus_tag="VW852_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271659.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRIYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPLTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3854..4780
                     /locus_tag="VW852_000469"
     CDS             3854..4780
                     /locus_tag="VW852_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271660.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4789..5856
                     /locus_tag="VW852_000470"
     CDS             4789..5856
                     /locus_tag="VW852_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271661.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5867..6793
                     /locus_tag="VW852_000471"
     CDS             5867..6793
                     /locus_tag="VW852_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271662.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(6868..8194)
                     /locus_tag="VW852_000472"
                     /pseudo
     CDS             complement(6868..8194)
                     /locus_tag="VW852_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8349..9059)
                     /gene="treR"
                     /locus_tag="VW852_000473"
     CDS             complement(8349..9059)
                     /gene="treR"
                     /locus_tag="VW852_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV0271663.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            9244..11211
                     /gene="treP"
                     /locus_tag="VW852_000474"
     CDS             9244..11211
                     /gene="treP"
                     /locus_tag="VW852_000474"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV0271664.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            11389..13014
                     /gene="treC"
                     /locus_tag="VW852_000475"
     CDS             11389..13014
                     /gene="treC"
                     /locus_tag="VW852_000475"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV0271665.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            13270..13518
                     /locus_tag="VW852_000476"
     CDS             13270..13518
                     /locus_tag="VW852_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV0271666.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            13725..14519
                     /gene="racE"
                     /locus_tag="VW852_000477"
     CDS             13725..14519
                     /gene="racE"
                     /locus_tag="VW852_000477"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV0271667.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRKKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            14516..15487
                     /locus_tag="VW852_000478"
     CDS             14516..15487
                     /locus_tag="VW852_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV0271668.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNSKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            15463..15984
                     /locus_tag="VW852_000479"
     CDS             15463..15984
                     /locus_tag="VW852_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0271669.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     FPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            15981..16442
                     /locus_tag="VW852_000480"
     CDS             15981..16442
                     /locus_tag="VW852_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0271670.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            16433..17173
                     /gene="xerD"
                     /locus_tag="VW852_000481"
     CDS             16433..17173
                     /gene="xerD"
                     /locus_tag="VW852_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV0271671.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPMALLSELEPLMGRTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            17173..17901
                     /locus_tag="VW852_000482"
     CDS             17173..17901
                     /locus_tag="VW852_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV0271672.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEIITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            17920..18489
                     /locus_tag="VW852_000483"
     CDS             17920..18489
                     /locus_tag="VW852_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV0271673.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            18476..19198
                     /locus_tag="VW852_000484"
     CDS             18476..19198
                     /locus_tag="VW852_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0271674.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            19198..19440
                     /gene="yidD"
                     /locus_tag="VW852_000485"
     CDS             19198..19440
                     /gene="yidD"
                     /locus_tag="VW852_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV0271675.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(19665..20630)
                     /locus_tag="VW852_000486"
     CDS             complement(19665..20630)
                     /locus_tag="VW852_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271676.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(20726..21478)
                     /locus_tag="VW852_000487"
     CDS             complement(20726..21478)
                     /locus_tag="VW852_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271677.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(21475..22431)
                     /locus_tag="VW852_000488"
     CDS             complement(21475..22431)
                     /locus_tag="VW852_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV0271678.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIKSVGGFLFFILLYRRSRQ"
     gene            complement(22421..23380)
                     /locus_tag="VW852_000489"
     CDS             complement(22421..23380)
                     /locus_tag="VW852_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271679.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFISSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(23803..24504)
                     /gene="galE"
                     /locus_tag="VW852_000490"
     CDS             complement(23803..24504)
                     /gene="galE"
                     /locus_tag="VW852_000490"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0271680.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHYTGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            24905..25969
                     /gene="pepA"
                     /locus_tag="VW852_000491"
     CDS             24905..25969
                     /gene="pepA"
                     /locus_tag="VW852_000491"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV0271681.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            25991..26287
                     /locus_tag="VW852_000492"
     CDS             25991..26287
                     /locus_tag="VW852_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0271682.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            26969..27412
                     /locus_tag="VW852_000493"
     CDS             26969..27412
                     /locus_tag="VW852_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0271683.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            27416..28123
                     /locus_tag="VW852_000494"
     CDS             27416..28123
                     /locus_tag="VW852_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV0271684.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     HAQYDIFRLKNGKIVEH"
     gene            28195..28923
                     /locus_tag="VW852_000495"
     CDS             28195..28923
                     /locus_tag="VW852_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271685.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSIHD"
     gene            28916..30436
                     /locus_tag="VW852_000496"
     CDS             28916..30436
                     /locus_tag="VW852_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV0271686.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQNHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            30781..31587
                     /locus_tag="VW852_000497"
     CDS             30781..31587
                     /locus_tag="VW852_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV0271687.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWAKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(31754..32290)
                     /locus_tag="VW852_000498"
     CDS             complement(31754..32290)
                     /locus_tag="VW852_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0271688.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            32423..33313
                     /locus_tag="VW852_000499"
     CDS             32423..33313
                     /locus_tag="VW852_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV0271689.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            33557..33910
                     /locus_tag="VW852_000500"
     CDS             33557..33910
                     /locus_tag="VW852_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV0271690.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            33911..34950
                     /locus_tag="VW852_000501"
                     /pseudo
     CDS             33911..34950
                     /locus_tag="VW852_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            complement(34997..36004)
                     /gene="galR"
                     /locus_tag="VW852_000502"
     CDS             complement(34997..36004)
                     /gene="galR"
                     /locus_tag="VW852_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV0271691.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAI
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEARINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            36168..37346
                     /locus_tag="VW852_000503"
     CDS             36168..37346
                     /locus_tag="VW852_000503"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV0271692.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAVRKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQKAGHVIDKGFDFYVYGNIPNGSGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLERDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            37365..38846
                     /locus_tag="VW852_000504"
     CDS             37365..38846
                     /locus_tag="VW852_000504"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0271693.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            38916..39320
                     /locus_tag="VW852_000505"
     CDS             38916..39320
                     /locus_tag="VW852_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV0271694.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            39477..40331
                     /locus_tag="VW852_000506"
     CDS             39477..40331
                     /locus_tag="VW852_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV0271695.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            40357..41127
                     /locus_tag="VW852_000507"
     CDS             40357..41127
                     /locus_tag="VW852_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV0271696.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            41114..41980
                     /locus_tag="VW852_000508"
     CDS             41114..41980
                     /locus_tag="VW852_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEV0271697.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(42067..43329)
                     /locus_tag="VW852_000509"
     CDS             complement(42067..43329)
                     /locus_tag="VW852_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV0271698.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(43329..43910)
                     /locus_tag="VW852_000510"
     CDS             complement(43329..43910)
                     /locus_tag="VW852_000510"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV0271699.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(44310..44405)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(44788..44916)
                     /locus_tag="VW852_000511"
     CDS             complement(44788..44916)
                     /locus_tag="VW852_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV0271700.1"
                     /translation="MKANYPIYRPLTKRKFRVSDHYIYPHEFAVLDPNGYFLRFSE"
     gene            complement(45027..45170)
                     /locus_tag="VW852_000512"
     CDS             complement(45027..45170)
                     /locus_tag="VW852_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV0271701.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            complement(45182..46009)
                     /gene="xth"
                     /locus_tag="VW852_000513"
     CDS             complement(45182..46009)
                     /gene="xth"
                     /locus_tag="VW852_000513"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV0271702.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            46143..46599
                     /locus_tag="VW852_000514"
                     /pseudo
     CDS             46143..46599
                     /locus_tag="VW852_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            46671..47048
                     /locus_tag="VW852_000515"
     CDS             46671..47048
                     /locus_tag="VW852_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV0271703.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            47254..>48027
                     /locus_tag="VW852_000516"
     CDS             47254..>48027
                     /locus_tag="VW852_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HEV0271704.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVL"
BASE COUNT        13991 a         8898 c        10867 g        14271 t
ORIGIN      
        1 aactgaatct tgaatactag aatgatactt ctacaatatt gttataattt aatttgaatt
       61 ccaaaatcac attgttccgt ccgtatttca ttttactgta tgtcttccta aactccaaag
      121 tttctgcgtc aaatttccta ttttcactgt agtctttgac acttcgaacg acaatttgaa
      181 tcatatattc aaaaaaaaat gaaaaatttg tgccaacaag cctaaatatt ctgaaaattt
      241 gtttgatttt taatggataa tgtgatataa tggttgtcaa tctataattc acataaacag
      301 aggagtagaa aatgaaaaaa aatcgtgtat ttgctacagc aggtcttgtt ttattagcag
      361 caggtgtact tgcagcatgc agttcttcaa aatcatctga ttcatcagcc cctaaagctt
      421 atggctatgt ttatacagca gacccagaaa ccttggacta cctgatttca agtaaaaata
      481 gtacaacagt agtgacttca aatgggattg atggtttatt cactaacgat aattacggta
      541 atcttgctcc tgcagttgca gaggattggg aagtctctaa ggatggtttg acctacactt
      601 ataagattcg taaaggggtt aaatggttta cctctgatgg agaagaatat gcagaggtga
      661 cggctaaaga ttttgtgaac ggtttaaaac acgcagcaga taaaaaatca gaagctatgt
      721 atttagctga aaattcggtt aaaggcttgg cagattatct atcaggaact tcaacagatt
      781 tttcaacagt tggtgtcaag gcggttgatg attatacgtt acaatacact ttgaaccagc
      841 ctgaaccgtt ctggaactct aagttgacct attctatttt ctggcctctg aatgaagaat
      901 tcgaaacatc aaaaggaagc gattttgcta aaccaacaga tccgacatcc ttgctttata
      961 atggtccatt cttgttgaaa gggttgactg caaaatcttc tgtagagttt gtaaaaaatg
     1021 agcaatattg ggataaagaa aatgtccacc tagatactat caatctagct tactatgatg
     1081 gatcagatca ggagtcgcta gagcgtaact ttactagtgg agcttatagt tatgcccgtc
     1141 tttaccctac cagctccaac tattctaagg ttgcagaaga atacaaggac aatatctatt
     1201 acacacaatc aggctctggg attgctggtc tgggtgtgaa tattgatcgc caaagttaca
     1261 actatacttc taaaactaca gattcagaga aagtagctac taagaaggca ttgcttaaca
     1321 aagatttccg tcaagccttg aattttgctc ttgatcgctc agcttactca gctcaaatca
     1381 atggtaaaga tggagcagct ttagcagttc gtaatttatt tgtaaaacca gactttgttt
     1441 cagctggtga gaagaccttt ggtgatttag tcgctgctca acttcctgct tatggtgatg
     1501 agtggaaagg tgtgaattta gctgatgggc aggatggttt attcaatgct gacaaggcca
     1561 aggcagagtt tgcgaaagct aagaaagctt tagaagcaga cggcgttcag tttcctattc
     1621 atctggacgt tccagtagac caagcatcaa aaaactacat atctcgtatt cagtccttta
     1681 aacaatctgt agaaacagtt cttggtgttg aaaatgtcgt tgttgatatt caacaaatga
     1741 caagtgatga attccttaat attacttact atgctgccaa tgcttcatct gaggattggg
     1801 atgtatcggg aggagtttca tgggggccag actatcaaga cccatctact tacctggata
     1861 ttttaaaaac aactagcagt gaaactacaa aaacatattt aggatttgat aatccaaata
     1921 gcccttcagt agttcaagtt ggtttgaaag aatacgataa attagttgat gaagctgcca
     1981 aagagacaag cgacttgaat gtccgttatg aaaaatatgc agcggctcaa gcatggttga
     2041 cagatagttc actctttatt cctgctatgg cttcttctgg tgcagcacca gtgctttcac
     2101 gaattgttcc atttactgga gcttctgcgc aaacaggctc taaggggtca gatgtttact
     2161 tcaaatattt gaaattacaa gataaagtgg tgactaagga agagtatgaa aaagctcgtg
     2221 aaaaatggtt gaaagaaaaa gctgaatcaa atgagaaagc tcaaaaagaa ttggcaagtc
     2281 atgtgaagta aataatttct aatagaactt tctctccatg aggagaaggt tcttttggga
     2341 tttttaaagg aaataatatg aaaaaatata tttttatgcg tgttttgcgg tcattggttt
     2401 cgattttctt agtaacgact ttgacctaca cgattatcta taccttggtt cctcgaaaat
     2461 tgattttcaa gcaggatcct aactataata aaattgcgac aacggctgat aaacgtgata
     2521 actatgaaaa tactgtgttt gagcgtatgg gctacattga gtattacgat actaaagagt
     2581 tgcaagaaaa ggcaagtagc atggattctt ctgtaacagt agaagcaaat gcgaccaata
     2641 aagctattta tgaaaagtac atcaatcaat taggtcatgg ttggactttg ggagaattta
     2701 ctgaaagtgg tcaattctat gctactcgtg aaattccaat ttttgaacgt gtttttcact
     2761 tctatgctaa cttgattgac attgaccata caaataaaat ccaagaccct gaaaatccag
     2821 acttgaaacg ctaccttcgt tttgaaaatg atccagctat cggatggtca ttggtcggtt
     2881 caggaactaa acataaatat ctcttgtact ttaacagtca gttcccattt gttcatcaaa
     2941 actttgtgaa cttgaattta ggtgactctt acccaaccta tgctaataca ccagttcttc
     3001 aggttattac tcaaggtcaa ggacaaacca aaactgccca agttcagttc ccaacaggta
     3061 agaaaacgtc ttctgtaaat atttactcaa gaatctacaa gtcacctagt caggctgact
     3121 ctcgtgaagt agctagctat gggaaagatg atccttatac agcgactgaa agtaattacc
     3181 aatatccatc tatgattgtc agctctgcta ttactggttt gattggtttg gttcttgcct
     3241 atgctcttgc cgtgccactt ggttcagcca tggctcgttt caagaacact tggattgata
     3301 gcctctcaac agggactttg accttcttgc ttgctcttcc aacgattgcc ttggtttaca
     3361 tcgttcgatt gattggatca tctattgccc ttccagattc attccctatc ttgggagctg
     3421 gagattggcg ttcttacgtt ttaccagcag tcatccttgg tttgttgggt gctcctctta
     3481 cagccatttg gattcgtcgt tacatgattg acttgcaatc tcaagacttt gttcgtttcg
     3541 ctcgtgcaaa aggtttgtct gaaaaagaaa tttcaaacaa acacatcttt aaaaatgcca
     3601 tggttccgct ggtttcagga attcctggtg ccattattgg ggttatcggt ggtgcaaccc
     3661 ttactgaaac agtcttcgcc ttcccaggta tgggtaaaat gttgattgac tctgtaaaag
     3721 catctaataa ctctatggtc gttggtcttg tcttcatctt tacatgtatt tctatcttct
     3781 cacgtctttt gggagatatt tggatgacta ttattgaccc acgtattaaa ttgactgaga
     3841 aaggaggcaa ataatgtcta caatcgataa agaaaaattt cagtttgtaa aacgtgacga
     3901 ttttgcctct gaaactattg atgcgccagc atattcttac tggaaatcag tgtttaaaca
     3961 atttatgaag aaaaaatcaa ctgtagtcat gttgggaatc ttggtagcca tcattttgat
     4021 aagtttcatc tacccaatgt tttctaagtt tgatttcaat gatgtcagca aggtaaacga
     4081 ctttagtgtt cgttatatca agccaaatgc ggagcattgg ttcggtactg acagtaacgg
     4141 taaatcgctc tttgacggtg tctggttcgg agctcgtaac tccatcctca tttctgtgat
     4201 tgcgacagtg attaacttgg ttatcggtgt ttttgtcggt ggtatttggg gtatttcaaa
     4261 atcagttgac cgtgtcatga tggaagttta caacgtcatc tcaaacatcc cacctctttt
     4321 gattgttatt gtcttgactt actcaatcgg agctggattc tggaatctga tttttgccat
     4381 gagcgtaaca acatggattg gtattgcctt catgatccgt gtgcaaatct tgcgctatcg
     4441 tgacttggaa tacaacttgg cgtcacgtac tttgggaaca ccaaccttga agattgttgc
     4501 caaaaatatc atgcctcaat tggtatctgt tattgtgaca accatgactc aaatgcttcc
     4561 aagctttatc tcatacgaag ccttcttgtc tttcttcggt cttggattac cgattacagt
     4621 gccaagtttg ggtcgtttga tttcggatta ttcacaaaac gtaacaacca atgcttactt
     4681 gttctggatt ccattgacaa cccttgtctt ggtatccttg tcccttttcg tagttggtca
     4741 aaacttagcg gatgctagtg atccacgtac acatagatag gagtagaaat gacaaaagaa
     4801 aaaaatgtaa ttttgactgc tcgcgatatt gtcgtggaat ttgacgttcg tgataaagta
     4861 ttgacagcca ttcgcggcgt ttcccttgaa ctagtcgaag gagaagtatt agccttggta
     4921 ggtgagtcag gatcaggtaa atctgttttg acaaagacct tcacaggtat gctcgaagaa
     4981 aatggtcgca ttgcccaagg tagtattgac taccgtggtc aggacttgac agctttatct
     5041 tctcacaagg attgggaaca aattcgtggt gctaagattg cgactatctt ccaggaccca
     5101 atgactagtt tggaccccat taaaacaatt ggtagtcaga ttacagaagt tattgtaaaa
     5161 caccaaggaa aaacagctaa agaagcgaaa gaattggcca ttgactacat gaataaggtt
     5221 ggcattccag acgcagatag acgttttaat gaatacccat tccaatattc tggaggaatg
     5281 cgtcaacgta tcgttattgc tattgccctt gcctgccgac ctgatgtctt gatctgtgat
     5341 gagccaacaa ctgccttgga tgtaactatt caagctcaga ttattgattt gctaaaatct
     5401 ttacaaaacg agtatcattt cacaacaatc tttattaccc acgaccttgg tgtggtggca
     5461 agtattgcgg ataaggtagc ggttatgtat gcaggagaaa tcgttgagta tggaacggtt
     5521 gaggaagtct tctatgaccc tcgccatcca tatacatgga gtctcttgtc tagcttgcct
     5581 cagcttgctg atgataaagg ggatctttac tcaatcccag gaacacctcc gtcactttat
     5641 actgacctga aaggggatgc ttttgccttg cgttctgact acgcaatgca gattgacttc
     5701 gaacaaaaag ctcctcaatt ctcagtatca gagacacatt gggctaaaac ttggcttctt
     5761 catgaggatg ctccaaaagt agaaaaacca gctgtgattg caaatctcca tgataagatc
     5821 cgtgaaaaaa tgggatttgc ccatctggct gactaggagg aaggaaatgt ctgaaaaatt
     5881 agtagaaatc aaagatttag aaatttcctt cggtgaagga agtaagaagt ttgtcgcggt
     5941 taaaaatgct aacttcttta tcaacaaggg agaaactttc tcgcttgtag gtgagtccgg
     6001 tagtgggaaa acaactattg gtcgtgctat catcggtcta aatgatacaa gtaatggaga
     6061 tatcattttt gatggtcaaa agattaatgg taagaaatcg cgtgaacaag ctgcggaatt
     6121 gattcgtcga atccagatga ttttccaaga ccctgccgca agtttgaatg aacgtgcgac
     6181 tgttgattat attatttctg aaggtcttta caatcaccgt ttatttaagg atgaagaaga
     6241 acgtaaagag aaagttcaaa atattatccg tgaagtaggt cttcttgctg agcacttgac
     6301 tcgttaccct catgaattct caggcggtca acgtcaacgt atcggtattg cccgtgcctt
     6361 ggtcatgcaa ccagactttg ttattgcaga tgagccaatt tcagccttgg acgtttctgt
     6421 acgtgcccaa gtcttgaact tgctcaaaaa attccaaaaa gagctcggcc tgacctatct
     6481 cttcatcgcc catgacttgt cggttgttcg ctttatttca gatcgtatcg cagttattta
     6541 caagggtgtt attgtagagg ttgcagaaac agaagaattg ttcaacaatc caattcaccc
     6601 atatactcaa gccttgcttt cagcggtacc aatcccagat ccaatcttgg aacgtaagaa
     6661 ggtcttgaag gtttacgatc caagtcaaca cgactatgag actgataagc catctatggt
     6721 agaaatccgt ccaggtcact atgtttgggc gaaccaagcc gaattggcac gttatcaaaa
     6781 aggactaaac tagtaatggt tttataattt ccatgtcaat ttttatggaa attaggctct
     6841 ttgtcaactg tagtgggttg aagtcagcta agctcgagaa aggacaaatt tcgtcctttc
     6901 ttttttgatg ttcagggcga taaaaatccg ttttttgaag ttttcaaagt ttcgaaaacc
     6961 aaaggcattg cgcttgataa gtttgatgag attattggtc gcttccagtt tggcgttgga
     7021 ataaggtaat tgaagggcgt tgacgatttt ctctttatct ttgaggaagg ttttaaagac
     7081 agtctgaaaa agaggatgaa cctgctttag attgtcctca atgaatccga aaaatttctc
     7141 cggttcctta ttctgaaagt gaaacagcaa gagttgatag agctgatagt gatgtttcaa
     7201 gtcttccgaa taactcaaaa acttgtttaa aatctcttta ttggttaagt gcatacgaaa
     7261 agtaggacga taaaatcgct tatcactcag tttacggcta tcctgttgaa tgagtttcca
     7321 gtagcgcttg atagccttgt attcatggga ttttcgatga aactgattca taatttggac
     7381 acgcacacga ctcatagcac ggctaagatg ttgtacaatg tgaaagcgtt caagaacgat
     7441 tttagcattc gggagtgaaa cagtctggga gactgtttca gcctgagcct agaaatttga
     7501 aagcgaagct gtttagccaa gtcatagtaa gggctaaaca tatccatagt aatgattttg
     7561 acgcgacatc ggacggctct atcatattta agaaaatgct ctcggatgac agcttgtgtt
     7621 ctaccctcaa gaacagtgat gatattgagc ttttcaaaat cttgcgcaat gaagctcatc
     7681 tccatctccc gattgaaaca gtcactcccc ggactgtttc aacgtcccaa gacataatct
     7741 taggcagatt tctaaaatta cactcaaagt ggaagtcatt gagctttcga atgacagttg
     7801 aagttgagat ggaaagctga tgggcaatat cggtcataga aatcttttca attaactttt
     7861 gagcaatctt ttggttgatg atacgaggga tttgatgatt cttcttgacg atagaagttt
     7921 cagcaaccat cattttagaa cagtgatagc acttgaatca acgctttcta aggagaattc
     7981 tagtaggcat accagtcgtt tcaagataag gaattttaga aggtttttga aagtcatatt
     8041 tcttcaattg gtttccgcac tcagggcaag atggggcgtc gtagtccagt ttggcgatga
     8101 tttccttgtg tgtatcttta ttgatgatgt ctaaaatctg gatattaggg tctttaatgt
     8161 ctagtaattt tgtgataaaa tgtaattgtt ccatatgatt ctttctaatg agttgttttg
     8221 tcgcttttca ttataggtca tatgggactt tttttctaca caaaataggc tccataatat
     8281 ctatagtgga tttacccact acaaatatta tagagccgaa aaaggctgag atctggtgtc
     8341 tcagtctttt atttcttgcg ttttgcaaaa tctacaaatc taaatttttc taacttatgg
     8401 cgactttcgg taaactgaaa ttggcgtccg tcttggagat agacttttga tttaatcgaa
     8461 acgacataag ggtctttgcc aatgtccatg agaatcttgt ctcggtcgct tgagtggtca
     8521 atggtgattt ccttctgagc ataatcaata aggagtttga ggccattttc tatataagaa
     8581 tagatagact gctcagcaat ttggcgagtg agatttggga ttagttccat atccagatag
     8641 tccgtatcca gaacggatac cagatcatcc accacacgct ggcggaccac cttccaaacc
     8701 atccgaaact ctgggaaacc ggttatcagt gaggattttt tatcaataat aatcttgtcc
     8761 agactgacca cgttggtttt agagcgcagt ccaagttctt taactagttc ttggtagctg
     8821 gttaggttgg atacagggaa attgacggtt tcttctttga cgacttgaga accttgccct
     8881 cttatctttt tgatcaatcc ttcctcttgg aggagagaca gggcctttcg gacggtatca
     8941 cgactcactt gatattgctc cataaggtcg tgctcgctag gaaggaaatc tccgacagcg
     9001 taagtctcgt tttgaatggt ttcttggatt tgcttaaata attgttggta tttcttcatt
     9061 ttgcctgctt aaataggttt ttagtctagt ttaatgaaca cctgtatcta ttttaccata
     9121 caagtgctag aaaatttaca aaaaaacacc ctttttatac caaacaagtt gcagacaagt
     9181 ttggtatcgt ttacaatata cttatcaaat caaacttgct ttgacaagta taaggagaat
     9241 caaatgggaa aatttgaaca agaagccaaa gatctgcttc aggcaattgg aggcaaagaa
     9301 aatgtgactg ccgtaactca ctgtgcgaca cggatgcgct ttgttttaag agatgataag
     9361 aaggctaatg ttaaagctat cgagtcaatt ccagctgtta aaggaacctt tacaaatgca
     9421 ggtcaatttc aggtaatcat tggaaatgac gtgcccatct tttataatga ttttacagcc
     9481 gtttcaggta ttgagggtgt ttccaaagaa gcagccaagt ctgcagctaa gagtaatcaa
     9541 aacgtggtcc aaggtgttat gaccactctg gcggaaatct ttactccgat tattccagcc
     9601 ttgattgttg gtggattgat cctcggtttc cgtaatgtct tggaaggtgt ccattggtcg
     9661 atgttggatg gcaagaccat cacagaatcc tctcagtttt gggcaggggt taatcacttc
     9721 ctctggttgc ctggtgaagc tatcttccag ttcttaccag tagggattac ttggtctgtt
     9781 tctcgtaaga tgggaaccag ccaaattttg ggaattgttc tcggaatctg tttggtatcg
     9841 cctcagttgc tcaatgccta tgcggttgct tcaacgccag cagctgatat cgcagcaaac
     9901 tgggtttgga attttggtta ttttactgtt aatcgtattg gttaccaagc ccaagttatc
     9961 ccagccttac ttgcaggttt gagtctgtct tatcttgaaa ttttctggcg caaacatatc
    10021 ccagaagtca tttccatgat ttttgtacct tttttgtcat tgattccagc cttgattttg
    10081 gctcacactg ttttgggacc aatcggttgg acaattggac aaggactttc atcagttgtc
    10141 ttggcaggtt taacgggtcc agttaaatgg ctcttcggtg caatttttgg tgccctctac
    10201 gctccatttg tcatcacagg tctgcaccat atgaccaatg ccattgatac acaattgatt
    10261 gcggatgctg gtggcactgc cctctggcca atgattgctc tttctaatat tgctcaaggc
    10321 tcagccgtgt ttgcctatta tttcatgcat cgccatgatg agcgtgaggc tcaggtttca
    10381 cttcctgcaa ccatttcagc ctatctcggt gttacagaac cagctctttt tggggttaac
    10441 gtaaaatata tttatccatt tgttgctggg atgactggtt cagcccttgc aggcatgtta
    10501 tccgttactt ttaatgtaac tgcggcttct attggtatcg gtggtttgcc aggtattctc
    10561 tctattcaac ctcaatacat gctgccattt gcaggaacta tgctagttgc gattgttgtt
    10621 ccaatgctct tgactttctt cttccgcaag gctggtctct ttacaaaaac agagggcgat
    10681 acgaacttgc aggcagaatt cgttgctcaa gaagaagcag aatttgtgag ccatgaacta
    10741 gtagaactta cttcggtaga aattatcagc ccactaactg gccaagtgaa agaattgagt
    10801 caagcgacgg atcctgtttt tgcatcaggt gtcatggggc aaggtctggt cattgaacca
    10861 agccaaggtg agttgacctc tccagttaat gggacagtga cggttctttt ccctaccaag
    10921 catgccatcg gcattgtctc tgacgaggga gttgaattgc tcatccacat cggtatggat
    10981 acagtaggtc ttgatggcaa aggttttgaa agtcttgtag tccaaggaga tcacgttaca
    11041 gttggtcagc aactgattcg ttttgatatg gatgtcatta aggctgcagg tctggtgaca
    11101 gaaactcctg ttatcatcac caaccaagat gcttatacag cgactattcc cggaacttat
    11161 ccgacaacga tccaagctgg agcatctctc atggtcgcta cacgaatcta atactcttcg
    11221 aaaatctctt caaaccacgt cagctctatc tgcaacctca aaatagtgtt ttgagcaacc
    11281 tgtggctagc ttcctagttt gttctttgat tttcattgag tataattgaa atagcacaaa
    11341 tagggacgga agactgcttc ctccctttta tcagaaaagg agaaacagat gacacttgat
    11401 aaaggaaaag tagtctatca aatctatcca aaatcttaca aagacaccac tgaaaacggt
    11461 attggggatt tccgtgggat tatcgaaaaa attccctatt tagccaaact aggtgtggat
    11521 atggtctggc tcaatccatt ctatccaagc cctcaacggg ataatggtta cgatatttca
    11581 gattatatgg cagtggatcc tctttttggt gatatggctg attttgagga aatggtgtgt
    11641 gtcggtaaag agcacaagat tgactttatg ctggacatgg tacttaatca ttgttcgaca
    11701 gagcatgaat ggtttcagaa agccctagct ggtgataagt attatcaaga ctttttcttc
    11761 atccaagacc aaccaacaga ttggcagtct aagtttggtg gttctgcatg ggcgcctttc
    11821 ggggatactg ggaaatacta tcttcaccta tttgatgaga cccaggctga ccttaactgg
    11881 cgcaattcca atgtccgtaa ggagcttttc aaggttgtta atttctggcg cgacaagggt
    11941 gtcaaaggtt tccgatttga tgtgatcaat ttgatcggta aggacgaggt ttcagtggac
    12001 tgtcctgaaa atgaagggaa gccagcttat acagacaaac ccatcgttca taactatttg
    12061 cgtatgatga accaagccac ttttggatct gacgatagct ttatgacagt tggggaaatg
    12121 tcttctacca ccatggaaaa ctgtgtcctc tattcgtcgc ctgatcgtca ggaattatcc
    12181 atgaccttta attttcatca cctcaaggtg gactataagg atggacaaaa gtggaccttg
    12241 gctccctttg attttgaaga gttgaagagt ctttaccata gttggggtaa ggaaatgagt
    12301 gataaagacg gttggagtgc cctcttttgg aacaatcacg atcaaccacg tgccttgaac
    12361 cgttttgtcg atattcaaaa cttccgcaag gaaggagcca ccatgctagc agccagcatt
    12421 cacctgtcac gtgggacacc ttatatctac atgggtgagg aaatcgggat gattgaccca
    12481 gactatgatt ctatggctga ttatgtggat gtcgaatcgc ttaatgccta tcagatgctc
    12541 ttagaagaag gaaagagtca gcaagaagcc ttccagatta ttcaagctaa gtcgcgtgat
    12601 aattcacgca ttcccatgca gtgggatgct tcggaaaatg caggattttc aacaggcact
    12661 ccttggttga aagctggtaa atcctataaa tacatcaatg tagaaaatga aatccagggt
    12721 ccgattttta ccttctatca agacttgatt cgactgcgta aggaaatgcc aatcatttca
    12781 gaaggaagtt acaaaccagc ctttgaagat agcaaacaag tctacgcttt tgaacgccag
    12841 tttgaggatc aaaagttact agtgctcaat aacttttatg ctaaagaagt ggaaatcgac
    12901 ttgccagcag tctaccaaaa tggacagatt ttgatatcaa actatgaaga tgcagaagtg
    12961 tctgagaaaa ttctactcaa accctatcaa acactggcta tttatgtaaa ttaatactct
    13021 tcgaaaatct cttcaaaccg tgtcagtttt atctgcaacc tcaaagcggt gctttgagca
    13081 acctgtggct agcttcctag tttgctcttt gattttcatt gagtataaac ttagcaagga
    13141 gacctctttt tcagaaaagc agggttcttt ttgctgtcta ttccatccct gaaagtgtag
    13201 aacaaactgg tcagggtggc tagtttgtgg tataatgaaa gggactcaaa aagaaacagg
    13261 agaaaaatga tggatttact tttagcaatt gtattgattg tgctagcttt tctaggagga
    13321 gctcttggag gaatgtactt ggttcgtaag caaatcgaaa aagaatttgc tgacaaccca
    13381 cgtttgaatg ctgaagcagt tcgtactctt ttgagtgcaa atggtcaaaa accaagcgaa
    13441 gctaaggtac aacaagttta ccaccaaatc atccgccaac aaaaggcagc ccttgctaac
    13501 aataaaaaga aaaaataaat aaggaaaagt ctgggatgaa agttccagac ttctcactat
    13561 gttggttatg gtttagggat gagacttttt ccttgcattc tattgatatt tgattatgat
    13621 taagagagag gtagttgaca ttctatcaag ctgtcggtca gttcgttcag tacaaggaat
    13681 cataatgaac gatcaatcag aaaaaagact agaaagaaga ctgtatggat aatcgaccaa
    13741 ttggtttttt ggattcgggt gtcgggggct tgaccgttgt gcgcgagctc atgcgccagc
    13801 ttccccatga agaaatcgtc tatattggag attcggcgcg ggcgccctat ggcccccgtc
    13861 ctgctgagca aattcgtgaa tatacttggc agctggtcaa ctttctcttg actaaggatg
    13921 tcaaaatgat tgtcattgct tgtaacactg cgactgcggt cgtctgggaa gaaatcaagg
    13981 ctcaactaga tattcctgtc ttgggtgtaa ttttgccagg agcttcggca gccatcaagt
    14041 ccagtcaagg tgggaaaatc ggagtgattg gaacgcccat gacggtacaa tcagacatat
    14101 accgtaagaa aatccatgat ctggatcccg acttacaggt ggagagcttg gcctgtccca
    14161 agtttgctcc cttggttgag tcaggtgccc tgtcaaccag tgttaccaag aaggtggtct
    14221 atgaaaccct gcgtcccttg gttggaaagg tggatagcct gattttgggc tgtactcatt
    14281 atccactcct tcgccctatt atccaaaatg tgatggggcc aaaggttcag ctcatcgata
    14341 gtggggcaga gtgcgtacgg gatatctcag tcttactcaa ttattttgaa atcaatcgtg
    14401 gtcgcgatgc tggaccactc catcaccgtt tttacacaac agccagtagc caaagttttg
    14461 cacaaattgg tgaagaatgg ctggaaaaag agattcatgt ggagcatgta gaattatgac
    14521 aaataaaatt tatgaatata aggatgacca ggactggtat gttgggtctt atagtatttt
    14581 tggtggcgtt aacagtttga gcgactataa gacagatttt cctctgtttg aattctccaa
    14641 aatatttgga gatgaagagt atggtttccc gctttcagtt actgttttac gctatggttc
    14701 tacctaccgt ttgttctcct ttgtggtaga catgcttaat caagaaatgg gacgaaactt
    14761 ggaagttatt caacgtcatg gggccctgct cttggttgaa aatgggcaac tcttgtatgt
    14821 agaattgcct aaagaagggg tcaatgttca tgatttcttt gagacaagca aggtcagaga
    14881 aaccttgttg attgcgactc gtaacgaagg taaaaccaag gaattccgag ctatctttga
    14941 taagttaggc tacgatgtgg aaaatcttaa tgactaccct gacctgcctg aagtagcaga
    15001 aacaggtatg acctttgaag aaaatgcccg ccttaaggca gaaaccattt ctcaattaac
    15061 gggcaagatg gttttggcag atgattctgg tctcaaagtc gatgtccttg gtggcttacc
    15121 aggcgtctgg tcagctcgtt tcgcaggtgt gggagcaact gaccgtgaaa ataattccaa
    15181 actcttgcac gaattggcca tggtctttga actcaaggac cgctcggctc agttccacac
    15241 aaccctagtc gtagccagcc caaataagga aagtttagtt gttgaagcag actggtcagg
    15301 ttatattaac tttgaaccta agggtgaaaa tggctttggc tatgatcccc tcttccttgt
    15361 aggagagaca ggtgagtcat cagctgaatt aaccctggaa gaaaaaaata gtcaatctca
    15421 ccgtgcctta gccgttaaga aacttttgga ggtatttcca tcatggcaaa gcaaaccatc
    15481 attgtaatga gcgattccca tggcgatagc ttgattgtgg aagaagtccg tgatcgctat
    15541 gtgggcaaag tcgatgccgt ttttcataac ggcgattctg aactacgtcc ggattttcca
    15601 ctttgggagg gcatccgcgt tgttaaaggg aacatggact tctacgccgg ctacccagaa
    15661 cgtctggtga ctgagcttgg ttcgaccaag attatccaaa ctcatggtca cttgtttgac
    15721 atcaatttca actttcaaaa gttggactac tgggctcagg aggaagaggc cgctatctgc
    15781 ctctatggtc acttgcatgt gccaagtgct tggatggaag gcaagatcct ctttctaaat
    15841 ccaggttcta tcagccaacc acgaggtacc atcagagaat gtctctatgc tcgtgtggag
    15901 attgatgata gttacttcaa agtggacttt ttgacacgag atcacgaggt gtatccaggt
    15961 ttgtccaagg agtttagccg atgattgcca aggagtttga gactttcttg ttggggcagg
    16021 aggaaacttt tttgacccct gctaaaaatc tagctgtgtt gattgatacc cacaatgcgg
    16081 atcatgcgac cctcttgctt agtcagatga cctatacccg tgttcccgtt gtgacagatg
    16141 aaaaacagtt tgttgggacg attggactca gagatattat ggcttatcag atggagcatg
    16201 acttgagcca agaaatcatg gcggatacgg atatcgttca tatgacaaaa acggacgtag
    16261 cggttgtttc gcctgatttc accattacgg aggtcttgca caagctagta gatgagtcct
    16321 tcttaccagt tgtggatgca gagggtattt tccaagggat tattacgcgc aagtccatcc
    16381 tcaaggccgt taatgccctc ttgcatgact ttagtaagga atatgagatt cgatgccaat
    16441 gagagacagg atttcagcct ttttagagga aaaacagggc ttgtctgtca attccaagca
    16501 gtcctataag tatgatttgg agcaattttt agacatggta ggtgagcgga tttctgagac
    16561 cagtctcaag atttaccaag cccagctagc caatctaaaa atcagcgccc agaagcgaaa
    16621 gatttcggcc tgtaaccaat ttctctactt tctctatcaa aaaggagagg tggacagctt
    16681 ttaccgcttg gaattagcca aacaagctga aaagaagacg gaaaagccag agattctata
    16741 cctagactct ttttggcagg aaagcgacca tccagagggc cgcttgctag cgctcttaat
    16801 cctagaaatg gggctcttgc ccagtgagat tttagccatc aaggttgcgg acatcaatct
    16861 ggattttcag gtgctgcgaa tcagcaaggc ttcccaacag aggattgtca ccattcccat
    16921 ggccttgctt tcagaattgg aacccttgat ggggcggacc tatctttttg aaagaggagg
    16981 gaaaccctat tctcgtcagt gggcctttcg tcagttagaa tcttttgtca aggagaaagg
    17041 ttttccatcc ttatcagctc aagtcttacg tgaacagttt attctaagac aaatagaaaa
    17101 caaggtcgat ttgtacgaaa ttgcaaaaaa attaggatta aaaacagtcc tgaccttaga
    17161 aaaatataga taatggatat taaattaaaa gattttgaag gacccctgga cttgctcttg
    17221 catctggttt ctaagtacca gatggatatc tacgatgtgc ccattacgga agtcatcgaa
    17281 cagtatctag cctatgtttc aaccctgcag gccatgcgtc tggaagtgac gggtgagtac
    17341 atggtcatgg ctagtcagct catgctgatt aagagtcgta aactccttcc gaaggtagca
    17401 gaagtgacag acttggggga tgacctggag caggacctcc tctctcaaat cgaagaatat
    17461 cgcaagttca agctcttggg tgagcacttg gaagccaagc accaagaacg ggcccagtat
    17521 tattccaaag cgccgacaga gttgatttac gaagatgcgg agcttgtgca tgacaagacg
    17581 accattgacc tctttttggc tttttcaaat atcctagcca agaaaaaaga ggagtttgca
    17641 caaaatcaca cgactatctt gcgggatgag tataagattg aggacatgat gattattgtg
    17701 aaagaatcct tgattggacg agatcaattg cgcttgcagg atttgttcaa ggaagcccag
    17761 aatgtccaag agatcatcac cctctttttg gcaaccctag agttaatcaa aacccaggag
    17821 ctgatcctcg tgcaagagga gagtttcgga gatatctatc tcatggaaaa gaaggaagaa
    17881 agtcaagtgc ctcaaagcta gacttgatag agaggaaaga tgagtacttt agcaaaaata
    17941 gaagcgctct tgtttgtagc gggtgaagat gggattcggg tccgccagtt agctgaactc
    18001 ctctctctgc caccgacagg tatccagcaa agtttaggaa aattagccca gaagtatgaa
    18061 aaggacccag attccagttt ggctttgatt gagacaagtg gtgcttatag attggtgacc
    18121 aagcctcaat ttgcagaaat tttgaaggaa tactctaagg cgcctatcaa ccagagcttg
    18181 tctcgggctg cccttgagac cttgtccatt attgcctaca aacagccgat tacgcggata
    18241 gaaattgatg ccatccgtgg ggttaactcg agtggagcct tggcaaagtt gcaggctttt
    18301 gacctgataa aggaagacgg gaaaaaggaa gtattggggc gccccaacct ctatgtgact
    18361 acggattatt tcctagatta catggggata aaccatttag aagaattacc agtgattgat
    18421 gagcttgaga ttcaagccca agaaagccaa ttatttggtg aaaggataga agaagatgag
    18481 aatcaataag tatattgccc acgcaggtgt ggccagtagg agaaaagcag aagagctgat
    18541 taagcaaggc ttggtgacgg ttaacggcca agtggtgcgt gaactagcaa ccactatcaa
    18601 gtcaggcgac aaggtcgaag ttgaaggtca acctatctac aacgaagaaa aggtctacta
    18661 tctgcttaac aaaccacgcg gtgtgatttc cagtgtgaca gatgataagg gtcgcaagac
    18721 ggttgtcgac ctcttgccca atgtcaaaga acgtatttac cctgtgggtc gtttggactg
    18781 ggatacatca ggtgtcttga ttttgaccaa tgatggggac tttacagacg agatgattca
    18841 ccctcgtaat gagattgaca aggtttatgt cgcgcgtgtt aaaggtgtgg ccaataagga
    18901 caatctccgc cccttgaccc gtggtcttga gattgatggt aagaaaacca agccagctgt
    18961 ttatgaaatt ctcaaagtgg acccagtcaa aaatcgctct gtggtgcagt tgaccatcca
    19021 tgaagggcgt aaccatcagg ttaaaaagat gtttgaagct gttggtctcc aagtagataa
    19081 gttgtctcgg actcgtttcg gacacctaga cttgacagga ctccgtccag gagaatcccg
    19141 tcgtcttaat aaaaaagaaa tcagccaact acacaccatg gctgtaacta agaaataatg
    19201 aaacgaattt taatagcgcc tgtgcgcttt taccaacgtt ttatctcacc agtctttcca
    19261 ccctcttgtc gctttgagct gacttgttcc aactacatga ttcaggctat tgaaaaacat
    19321 ggttttaagg gggtattgat gggcttggct cggattttac gttgtcatcc ctggtcgaaa
    19381 acaggtaagg accccgttcc agaccacttt tcccttaaac gaaatcaaga aggggaatga
    19441 ggtggggtaa atagatttca aaatgataaa aacgcatcct atcaggtttg agtgaatttg
    19501 ataggatgcg ttttagaatg tcaaaatttt atactcttcg aaaatccctt caaaccgcgt
    19561 ctgctttcat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
    19621 tgctctttga ttttcattga gtattaaatt gagtttgaag tggcttattt caaagctttt
    19681 tgtatgtctt caatcatgag ttttgttgat tcaagtccgc ctccgcttag ataccagagg
    19741 tctggtgtta gttggataat cttaccattt ttagcagcag gtgtttcagc gataagggca
    19801 ttttctagga caccgtcgtt gctagagttg tccccaccga tggcaagggt acggttgatg
    19861 acaaagagga tgtcagggtt gatttctttg acactttcaa agctgacttc ttgtccgtgg
    19921 cgtgagtctt caaatgttgt atcagttggt ttgaatttca aggtttggta caagaaagag
    19981 aaacgagatt tggcaccaaa ggctgccatt tttccttcat taaggaggat cgcaagggcc
    20041 tttttgtcag agctttcatt tttagtagcg acttcttgga tgctcttgtc tagcttggcc
    20101 aattcttcct tggctttctg tgtaccagtt tcgccgaagg cacttgctaa ggattcgata
    20161 ttagccttgg tagaagtcca gtagtcgtcc ttgcttgctt ggaagagaac ggttggggcg
    20221 atttctttga atttgtctac gaatttttgt gtacgtggtg aagcgataat caaatcaggc
    20281 tcaagggcgg cgatagcttc taaatcaggt tctttcatag aaccaacatt tttgacagtt
    20341 cccactaggt cttttagata agtcggaaca gtttttgtag gcattccgac gatatttttt
    20401 tcaaatccta aagcgcgaat agtatccgca gcgccgaggt caaaggtcac aatcttttca
    20461 ggaactttgg aaagtttgac ctcgtccagt gaacttttaa tggttacctc tgttggagca
    20521 gagctactgg tctctgtctg actagtgctt gagtttgtac tacatgcacc aagtaggagc
    20581 aagaagctgg ccactagggc agtgaaataa agtttaaggg atgttttcat aatttctcct
    20641 ttttaaaatg tgataacgat ttagggagtc tcttgatctt attgattaag agactgaagg
    20701 ttctctaact tgagctttta tgttactagc tatagataca gatctttttg tcattgatat
    20761 cagctagcgt gatgggaatc tcataaagtt ggctgagcag gtcagcctgc atgatttgat
    20821 cggtgcttcc cttgctaaag acctgaccgt ccttgaaggc gacaatttca tctgcatact
    20881 gactggccat gttgatatcg tggaggacga tgataatggt cttgccgagt tcctccacca
    20941 gtcgtcgaag aatttgcatc atgctgacgc tttgcttgat atcgagattg ttgagtggtt
    21001 cgtccagcaa gataaagtcc gtatcctggg ccagtaccat agcgataaag acgcgctgga
    21061 gttgcccccc tgacaggcta ttgatgtagc ggtcttttaa gttggtcagt tccaaatagt
    21121 tcagagtttc tcggattttt tcccagtctt ctgatctaag tcgacctcgg ctgtagggaa
    21181 aacgtccaaa actgaccagt tcttcaacag tcaatttggc ttggtaattg attttctgtt
    21241 ttaggatggt tagttcttgg gccagttctt gcgaattcca gctctcgatt tcacgtcctt
    21301 tgatactgag aactccctga tctttcttgg ttagtctgct catgatggag aggagagtcg
    21361 attttccagc accatttgga ccaataaagg ctgtcagttt ttgaggactg acttcaagcg
    21421 aaatgccttg caaaatatcc tgtttttgaa tggatttgtc aatgttttcc agtttcactg
    21481 acgagacctc ctatatagta agataaagaa taagaagcca cccacactct taatgatcat
    21541 actgatacga atttccagtg caaagactcg ttcaatcaag gcttgcccca aggttaagct
    21601 aataaatcca accagaatgg ccactataaa gagtaacttg tgctgatagt ctttgacaat
    21661 caggtaggtg aggttggcca gcataaagcc gaagaaggcc ataggtccta ccaaggcagt
    21721 ggccgttgag gtcaaaagca cgattcccca gaggagctct ttctgttctt tttcaacatc
    21781 gagtcccaat atctgagccg tttctctttg caggtgcaag acatctagaa cgactgcttt
    21841 tcgaaagaaa aagattgtca aagcgaggat gatcagagaa ccgatggcta ggatggaagt
    21901 gttgagatgt tgaaaggagg caaaaagact attttgcagt ttatcgtatt cgtttggatc
    21961 cattaggact tgaaggaagg tactgatatt tcgaaagaga cttctgagcg ctagacagat
    22021 cagcaggatg aagaccaggt cttgcttcat cagtgtcttc aagtaacctt gtaaggcgag
    22081 aaagaagagg gactggacaa gaagtaagac taggaattct aagatagggg atttgccaag
    22141 ttgaagaaac ttgctttcaa aaaccagtag tagggtttgt agtaggacgt agaaggattc
    22201 aattcccaaa atactaggcg tcaggaagcg attttccgtc agggtttgaa aactaatggt
    22261 cgaaatccca gtcgcgatgg ctaccaagag ataaacgatg atcttttggg aacgcaactt
    22321 ccaagcaaag gctgacaagt gagtgatggg ccaaaagtag agaagacaag ctccgatggc
    22381 aagaataatg agaatccaga agagcttggt atgtttgctt ttagtctgca tcttttcgtc
    22441 cccctctcca gagaagtagg ataaagacga gactaccgat gattcctagc aagagactga
    22501 cagacaactc atagggccta atcagaactc gggataggat atcgcaagcc agaactagat
    22561 tggcaccaac cagtgcgacc atgagtttgg tttgacttag attatctcca tagcgcttgc
    22621 gaacaagatt gggaacgata actccgagaa atggtaagcc acccacggta atcatggtga
    22681 cgcttgtcgt tagcgccacc agaaagaggg ctagtttttc aagtagggag taggaaatcc
    22741 ccaaactctc gctggtttct ttccctagat tcatgatggt gaaggtttgg gataatttcc
    22801 aaacggttat caggatgatg aggcctaaga agagccactc atactgatgg gtctgaatca
    22861 tggagaagga gccctgggtc caggcagtca tactctgaac cagattgaaa cgataggcga
    22921 taacttctgt gactgagccg ataattccgc tatagatgat cccaatcaga ggtaacatcc
    22981 acctttcctt tacagtaaaa atggtcataa aggctaggaa gaagagggtg aatacgatgg
    23041 atgaaataaa agcgaagagc atcttttggg tcagactagc cgatggaaag acaaaaaggc
    23101 tcagcaccat ccccagtttg gcggcttcag tcgttccaac tgtactcggt gcagcaaact
    23161 gattttgggt aatagtctgc atgagaaggc ctgccatact catactagag gcagtcagga
    23221 gaatactgat agttcttggg agacgggact cttgaaagag gagccaggtc tgctggtcga
    23281 aatcaaatag ctttccccat gaaaaatcac tggtcccaat gctaatagag agaaagacta
    23341 ggagtagaag taagccaatt aaataatgag aaagtttcat accccgtcct tttatgtaga
    23401 tttggtatcg aaagatacct gcggatataa atgtaacatt atttttctaa tctgtcaata
    23461 aaatttctga caatttaata aatacaacaa ggagagagca acaagacttt ctcctttgtt
    23521 atcctattct aaaatgtttt taccttaatc tgataaaata atatcttcga gggagtagct
    23581 agccgtccaa tcaagatatt gtttagcttt tgaagcatct actaggacac tggctgggtc
    23641 actagcacgt cgagcaacaa tctcgtgtgg gattttttaa tttagtaatt cttcagcagt
    23701 tttaaagatt tctttgatag tatagccttt tttagttcct aagttaaaga tttgagaaga
    23761 actgtcttct tgaaataggt agttcattcc tttaacatga gcctatgcaa ggtccaagac
    23821 ataaatgtaa tctcgaatac atgaaccgtc acgtgtatcg tagtcatctc caaatatttt
    23881 taagctatca ttttgtccca atgcggtctt gttgatattt ggaatgatgt gagttggatt
    23941 tttcacacgc agaccgtttg aagcatccat ttcagcccca gcaacattaa agtaacggaa
    24001 aataacatat ttccagtcgt agcgattggc catccagtaa atcattcgtt cgcccatcag
    24061 ttttgtctct gcataagggt tgacagggtc gagcagggta tcttcagtca ccggcttgtc
    24121 aatacagtta tttccataga gagaagcagt cgaagagaac atgatttttt gaatgccaac
    24181 ttcagataag actttgagaa cttggttcat accagcaacg ttggcagtga agtatttact
    24241 tggattttca atactttcgc tcacaacaat ttcacctgta taatggagga cagcatcaat
    24301 ctgattttct tctaagtaag cctttaaggc gctagcatca taaacatcca gttctttaaa
    24361 gctagcacga ctatccacag ccgctcgatt tcctgtggag agattatcga ggacatgcac
    24421 cttataacca gcatttaaaa gagctttaac ggtatgggag ccaatgtagc tagtcccacc
    24481 tgtaacaaga attgttttgg tcatgatttt ttccttttct agttttgtac tatagtgaat
    24541 tgaaataaga tatgaacaac tctattagga aagtcaaatt aatttctaga aatgttttag
    24601 cagctacagc gtactattcc aaactcaacc aactatatat ttaaagaaag aagaaaggga
    24661 agtcaactat tttctgtaaa tttcatgaaa aatccaaaca actttgatgg tgtattgttt
    24721 ttttgtttgg aaataaatat tcgagttaat tttggaatgt ttactagatt ttgcactaca
    24781 tgcaagagaa tactatcctt ttctaagata gagcaggact aagatactaa cttagaaatc
    24841 caccttccca ctatccttct cctataaaaa gtggtaaaat ggatgaaaga aagaaggaac
    24901 tgaaatgaca acattatttt caaaaattaa agaagtaaca gaacttgctg cagtctcagg
    24961 tcatgaagcg cctgtccgtg cttatcttcg tgaaaagttg acaccgcatg tggatgaagt
    25021 ggtgacagat ggcttgggtg gtatttttgg tatcaaacat tcagaagctg tggatgcacc
    25081 gcgcgtcttg gtcgcttctc atatggacga agttggtttt atggtcagcg aaatcaagcc
    25141 agatggtacc ttccgtgtcg tagaaatcgg tggctggaac cccatggtag ttagcagcca
    25201 acgtttcaaa ctcttgactc gtgatggtca tgaaattcct gtgatttcag gttctgttcc
    25261 tccgcatttg actcgtggaa aggggggacc aaccatgcca gccattgccg atatcgtttt
    25321 tgatggtggt tttgcggaca aggctgaggc agaaagtttt ggcatccgtc ctggtgatac
    25381 catcgtacca gatagttctg caattttgac agccaatgaa aaaaatatca tctcaaaagc
    25441 ttgggataac cgctacggtg tcctcatggt aagcgagcta gctgaagctt tatcaggtca
    25501 aaaactcggc aatgaactct atctgggttc taacgtccaa gaagaagttg gtctgcgtgg
    25561 cgctcatacc tctacaacca agtttgaccc agaagtcttc ctcgcagttg attgctcacc
    25621 agcaggtgat gtctacggtg gtcaaggcaa gattggagat ggaaccttga ttcgtttcta
    25681 tgatccaggt cacttgcttc tcccagggat gaaggatttt cttttgacaa cggctgaaga
    25741 agctggtatc aagtaccaat actactgtgg taaaggcgga acagatgcag gtgcagctca
    25801 tctgaaaaat ggtggtgtcc catcaacaac tatcggtgtc tgcgctcgtt atatccattc
    25861 tcaccaaacc ctctatgcaa tggatgactt cctagaagcg caagctttct tacaagcctt
    25921 ggtgaagaaa ttggatcgtt caacggttga tttgattaaa cattattaaa ccataaggga
    25981 ggtggtaggg atggcagaac caaacctaga aagccttata aaagatcttt acaatcatgc
    26041 tcgacatgat ttgagtgaag atttagttgc tgctctccta gagactgcta aaaaactgcc
    26101 tactacaaat gagcaattgc aggcagttcg tctctcaggc ctggtcaatc gtgaattgct
    26161 cctaaatccc aaacatccag cacctgagtt gctcaacttg gctcgcttta tcaaaagaga
    26221 agaagccaag tacagaggaa ctgcgacttc tgcgcttatg tatgaggaac tttttaaaat
    26281 gctttgattc cattgtggat caaagcattt tttataagac ctatctcgtg agcgttcaaa
    26341 tagtagtaac aaggaatcat gagaagtcag ccgagaccat agtagtgaag aaacttctgt
    26401 aatggaagtg gagtgaagtg aggaatcgtc aaacggtagg ggataggctg tttgaagggt
    26461 gctacgaaaa agatagggta aggtgaaatt atagcggaat gaaataagat atgaacaaat
    26521 tgattaggga aatcaaatga atttatagaa atcttttagc agtcatggtg tcctattcca
    26581 gtttcaaaat gctataaata cgaacaaatt gattgggaaa ttcaaatcgc tttctatcaa
    26641 tgttttagaa gatacagtgc tctagtctaa cttcaatata ctataaaaga acagatggaa
    26701 gcaattatga tttacttcta tctttaaaat aaatggtctg ataggaaaaa tgtgtcccct
    26761 cataaaatat aggatagtac gaatggaagg cttttgtaga ctggaggacg aaggagttta
    26821 gaaactctcg ttaaaagtta gttgagccgg agtatggtgg tgtggataat tatcaaaaat
    26881 tatttctaaa tcgaaatagt tgacagttag aacgatgagt gttacaattg ttttattcat
    26941 ttcgatatcg aaataaattg gaggtgttat gaaagatggt catttgctag cccatcatat
    27001 tcgtttgttg aatgggcgga tttttcaaaa gttactgagt caagatcctg aggctcttta
    27061 taggggtgaa cagggcaaga ttttagcggt tttatggaat agtgaaactg gctgcgcaac
    27121 tgcgacagat atcgcgcttg cgactgggct tgcgaataat acgctaacga ctatgataaa
    27181 aaagctagag gaacaaaagc ttgtaattgt tagtccgtgt ggaaaagaca agcgtaagaa
    27241 gtatttagtt ttaacggagt taggcaagtc ccagaaagaa gtggggcatc gtgtcagtca
    27301 gaaattggat actatctttt acaaaggatt ttcagaggaa gaaattcacc aatttgaagg
    27361 ttttcaagaa agaattttgg cgaatctgaa agagaaggga aatgaggttt agaatatgtc
    27421 acaacaagtg aaaaatgctc acaacctata catccatgcc attcaagacg gacgagttgc
    27481 tgaggctcaa gcccagtctg taggggatac ctatatccaa cattcgacag gtgttcctga
    27541 cgggaaagaa ggatttgcag ccttctttgc agatttcttt gagcgtcatc cagagcgtca
    27601 gattaagatt gtccgcacca ttgaggatgg caatctggtc tttgttcatg ttcatcaata
    27661 tctgaatggt ggagaagctc aatgggtgac gacggatact ttccgtgcgg atgagaatgg
    27721 tcgtattgtt gagcattggg atgtcgttga ctactatcga acacctgaaa atgaccaact
    27781 agatcaaatt tttggagatt ttgaaatcaa ggatttggac aagaaagcag aaaataaaaa
    27841 gctggttcgc cgtttcttga cagaaatttt ccaaaatggg gagctagagc agtggagtga
    27901 ttatgtggca gacgatttga ttcagcataa tcatgatatt ggacaaggaa gtgctgctta
    27961 taaaaactat gtggctgaat atagtgtcac ttttgacttt gttttccaac tcttaggaca
    28021 aggaaactat gtggttagct atggtcagac tcagattgat ggcgttgctc atgcccagta
    28081 cgatatcttc cgtttaaaga acgggaaaat tgtggagcat taggataata aggaagttat
    28141 gcctaaggta gaagacttga ccaatcgagg gaagttttaa attgaggaca aagaatgatt
    28201 gaatacaaaa atgtagcact gcgctacaca gaaaaggatg tcttgagaga tgtcaactta
    28261 cagattgagg atggggaatt tatggtttta gtagggcctt ctgggtcagg taagacgacc
    28321 atgctcaaga tgattaaccg tcttttggaa ccaactgatg gaaatattta tatggatggg
    28381 aagcgcatca aagactatga tgagcgtgaa cttcgtcttt ctactggtta tgttttacag
    28441 gctattgctc tttttccaaa tctaacagtt gcggaaaata ttgctctcat tcctgaaatg
    28501 aaggggtgga gcaaggaaga aattacgaag aaaacagaag agcttttggc taaggttggt
    28561 ttaccagtag ccgagtatgg gcatcgctta cctagtgaat tatctggtgg agaacagcaa
    28621 cgggtcggta ttgtccgagc tatgattggt cagcccaaga ttttcctcat ggatgaaccc
    28681 ttttcggcct tggatgctat ttcgagaaaa cagttgcagg ttctgacaaa agaattgcat
    28741 aaagagtttg ggatgacaac gatttttgta acccatgata cggatgaagc cttgaagttg
    28801 gcggaccgta ttgctgtctt gcaggatgga gaaattcgcc aggtagcgaa tcccgagaca
    28861 attttaaaag cgcctgcaac agactttgta gcagacttgt ttggaggtag tattcatgac
    28921 taatttaatt gcaacttttc aggatcgttt tagtgattgg ttgacagctc tatctcaaca
    28981 tttgcagttg tcacttttga ccttgttact agctattttg cttgcgattc ccttggctgt
    29041 ttttcttcgc tatcatgaga agctggccga ctgggtcttg cagattgcag gtattttcca
    29101 gaccatcccg tctctggcct tgttggggct ctttatccct ttgatgggaa ttgggacctt
    29161 gccggctttg acagctctag tgatttatgc gatttttccg attttacaaa atactattac
    29221 tgggctgaag ggaattgatc cgagtcttca agaggctggg attgcctttg ggatgaccag
    29281 atgggagcgt ctcaagaagt ttgaaattcc acttgccatg cctgttatca tgtctggaat
    29341 tcggacggca gcggtcttga ttatcggtac ggctaccttg gcgaccttga ttggggcagg
    29401 gggactgggt tcctttatcc tgttgggaat tgaccgtaat aatgccagtt tgattttgat
    29461 tggggccctt tcttctgcag tgctagctat tgcctttaac ttcctactaa aagtaatgga
    29521 aaaagcgaaa ttgcggacga ttttctctgg ttttgccttg gtggctttat tactgggtct
    29581 gtcttatagt ccagcccttt tggttcaaaa agagaaggaa aacttgatta ttgctgggaa
    29641 aataggtcca gaaccagaaa ttttggctaa tatgtataaa ttgctgattg aagaaaatac
    29701 cagcatgact gcgactgtta aaccgaattt tgggacgaca agtttccttt atgaagctct
    29761 gaaaaaaggt gatattgaca tttatcctga atttaccggt acggtgactg aaagtttgct
    29821 tcaaccatca cccaaggtga gtcatgagcc agagcaggtt tatcaagtgg cgcgtgatgg
    29881 cattgccaag cagaatcatc tagcctatct caaacctatg tcttatcaaa atacctatgc
    29941 tgtagctgtt ccgaaaaaga ttgctcaaga atatggcttg aagaccattt cggatttgaa
    30001 aaaagtggaa gggcagctga aggcaggctt tacgcttgag tttaacgacc gtgaagatgg
    30061 aaataagggc ttgcaatcaa tgtatggtct caatctcaat gtggcgacta tgcagccagc
    30121 tcttcgttat caggcaattc agtcagggga tattcaaatc acggatgcct attcgactga
    30181 tgcggaattg gagcgttatg atttacaggt cttggaagat gacaagcaac tcttcccacc
    30241 ttatcaaggg gctccactca tgaaagaagc tcttctcaag aaacacccag agttggaaag
    30301 agttcttaat acattggctg gtaagattac agaaagccag atgagccagc tcaactacca
    30361 agtcggtgtt gaaggcaagt cagcaaagca agtagccaag gagtttctcc aagaacaagg
    30421 tttgttgaag aaatgatggg acagagactt taaacatact gaactcaact tccttaaacg
    30481 actagataaa agaatgctca gacaatttcg tctgggcttt tttgatgtga gaatgactga
    30541 atgtcactta aaatggaaag aaagagaaaa ttttcatgat tttctaaaat tttactgtaa
    30601 agacagttga ctattcataa aataggtgta taatattttg tgaactactt aacaaataag
    30661 aggtttcagc tcatgtcgac taaggttaga aatcttgtgt ataggcagtt cattatctaa
    30721 tagagcgttg cgtccgaaag tctatccaga cacggctctt taaaaacaaa aggagaaatg
    30781 atgcatactt atttgcaaaa gaaaattgaa aatatcaaaa caaccctagg tgaaatgtca
    30841 ggtggttacc gtcgtatggt tgcggctatg gctgatttag gattttcagg aactatgaag
    30901 gctatctggg atgacctctt tgcccatcgt agttttgccc agtggattta tttgctggtt
    30961 ttaggaagtt ttcctctctg gctggagttg gtttacgaac atcgtattgt tgactggatt
    31021 gggatgattt gtagcttgac agggattatc tgtgtaatct ttgtatcgga aggtcgagca
    31081 agtaattatc tttttggctt gattaactct gttatttacc ttattttggc cctacagaaa
    31141 ggcttttatg gtgaggtgct gacgacactt tacttcacag tcatgcagcc aattggactt
    31201 ctagtttgga tttatcaggc acagtttaag aaggaaaagc aggagtttgt cgcgcgtaaa
    31261 ctggacggca agggctgggc aaagtatctt tccattagtg tgctttggtg gttggccttt
    31321 ggcttcattt atcagtctat tggtgccaat cgtccctatc gtgattcaat cacagatgca
    31381 accaatgggg tagggcaaat cctcatgaca gctgtttacc gtgaacagtg gatattctgg
    31441 gcggctacca atgttttttc aatctatctc tggtggggag aaagcctgca aattcaaggg
    31501 aaatatctaa tttatctcat taacagtcta gttggttggt atcaatggag caaggcagct
    31561 aagcagaata ctgatttact taactaggaa aagatgtttg aaagtgctgt tttgagattt
    31621 cgattaaaac agatatagtt gataatcaag gatttatagt atgaaaaaga ggatcggcgg
    31681 gtcctctttt gttgttgaaa agataaaaaa ctcagtaacc tagaaataag acaactgaag
    31741 ctttactcta tattcaattt ttaggaatga gaaggtctag ataaaattgg acaacttcct
    31801 ggtctgtgaa atcttgacct tttttgagcc accaggtcaa tgtctcgata aagttggaca
    31861 tgaccaagtg ttggaggtaa gaagtaggca tattagggtg ggcttctttt aaattatcag
    31921 ctagcacgga atagacatgg tgttctagct ctttatggag ttgacggagg aagtagtcat
    31981 ttttggaaaa tagcagactg gtgatatggt cttggttttt atgaaaatgg agaaagaggt
    32041 gggcgaggta gtcctcggtt gaaatggctt gctctctttc aaaaagatga tggaagaggt
    32101 agcggcagag ctggtccaga agaagctcct tactttcata gtgacagtaa aaggtggatc
    32161 ttcccacatc tgcgagatca atgatatcct gaacagtagt ggcctcgtag cccttagcat
    32221 tcaaaagttg tataaaagct tgatagatgg cttttttggt tttgctgata cggcggtcaa
    32281 tgttagtcat atggacactt aaggcaaatt gttcagaact gaataaagct gacgttttgc
    32341 ttctatcctt tctttgagtt ttagtggata atgataatga acaaggtgtt cataaaccta
    32401 ttataacaaa ggaatgagaa atatgaaggc aaaatatgct gtttgggtgg cttttttctt
    32461 aaatttgact tatgccattg ttgagtttat tgcaggtgga gtatttggtt ctagcgctgt
    32521 tcttgctgac tctgtgcatg acttgggaga tgtgattgca attggaatat cagcttttct
    32581 agaaacaatc tccaatcgtg aagaagacaa tcagtacacc ttgggctata agcggtttag
    32641 cctgctagga gccttggtaa cagctgtgat tctcgtaaca ggctctgttc tagtcatttt
    32701 ggaaaatgtc acgaagattt tgcatccgca accagtcaat gatgagggga ttctctggtt
    32761 aggaattatt gcgattacta tcaatctgtt agcgagtctg gtggttggta agggaaagac
    32821 aaagaatgag tctattctga gtctgcattt tctggaagat acgctagggt gggtagctgt
    32881 tatcctgatg gcgattgttc ttcgatttac ggactggtat atcctagatc ctcttttgtc
    32941 ccttgtcatt tctttcttta ttctttcaaa agccctttca cgtttttggt ctacactcaa
    33001 gattttcttg gatgctgtgc cagaaggtct tgatatcaag caagtaaaga gtggcctgga
    33061 acgattggac aatgtggcca gccttaatca gcttaatctc tggactatgg atgctttgga
    33121 aaaaaatgcc attgtccatg tttgtctaaa agaaatggaa catatggaaa cttgtaaaga
    33181 gtctattcga attttcctaa aagattgtgg ttttcaaaat attaccattg aaattgatgc
    33241 tgacctagaa actcaccaaa cccataagcg aaaggtgtgt gacttggaac ggagttatga
    33301 gcatcaacat tagaaaaaag tgaaaaatac ttgggtacta tcttatttgg aatagagtaa
    33361 tttctttatt atttaaatat ttcaaaaatt ggtaagagaa gagcattgta taaactccag
    33421 atatatgatt gttaatgata aaaatttttc gattagatac aaaatgcttg acttggagtc
    33481 aactcaaagt tatataataa gataagtgag ttagaatagc gtgaattcag tgaatgaaat
    33541 gagaggaggt tagcgtgtga atattaaatc tgccagtgat ttgttgggaa tttcagcgga
    33601 tacgattcgg tattatgaac gggttggtct tgtgccaccg attactcgta ctgctactgg
    33661 gattcgtgat tttcaagatc aggatatcga agcgctggaa tttattaagt gttttcgttc
    33721 ggcgggtgtc tctgtagata gtttagttga ctatatgtcg ctctaccaaa agggagatga
    33781 aacgagagag gagaggcttg gtattttaga agaggaaaag caaaaattag aggagcgctt
    33841 gtctcagcta cagacagctt taaatcgttt aaatctcaaa attaaacttt ataaggaagg
    33901 aaaattttaa atgaaatcag cagtatatac aaaggcaggt caggttggac ttgttagcat
    33961 tgaacgtccg caaataatag aagcggatga tgtgattatt cgtgtggttc gttcgtgcgt
    34021 ttgtggttca gatttatgga ggtaccgtaa tccagaaacg aaagctggac acaaaaatag
    34081 tggacacgaa gcgattggga ttgttgaaga agctggggaa gccattacga cggtgaaacc
    34141 aggtgatttt gtgattgtcc cttttacaca tggatgtggt gagtgtgatg cctgtcttgc
    34201 tggatttgac ggttcttgcg acaatcatat tggcaataat ttggggggtg attttcaggc
    34261 agaatatatt cgcttccact atgcaaactg ggcgctggtt aaaatccctg gtcaaccttc
    34321 tgactataca gaagggatgc tcaagtccct tttgactctt gcagatgtca tgccgacagg
    34381 ctatcatgcg gggcgcgtgt tgcaaatgtt caaaaagggg acaaggttgt tgttattggt
    34441 gatggggctg ttggtcaatg tgctgtcatc gcggctaaga tgcgtggagc atcacaaatt
    34501 atccttatga gccgtcatga agaccgtcaa aagatggcta tggagtcagg tgcgacagct
    34561 gttgttgcag aacgtggtca agaaggaatt acaaaggtgc gtgaaatcct cggtggagga
    34621 gcagatgcag cacttgaatg tgttggtacg gaggctgcta tagaacaggc gctaggtgtt
    34681 cttcataatg gagggcgtat gggctttgta ggagtcccac actataataa tcgtgctctt
    34741 ggttcgacat ttatgcaaaa tatctctgta gcaggtgggg cagcttctgc tacaacatac
    34801 gataagcaat ttttactaaa agccgtcctt gatggtgata tcaatccagg tcgcgtcttt
    34861 acttcaagtt ataaactgga agatatcgac caagcctaca aagatatgga tgaacgtaag
    34921 accattaagt ctatgattgt aatcgaataa aaaacgaata ggagttctaa aactcctatt
    34981 cgttttttat gttatcctat tcttgattta gggtactttc tcttaatgtc agtctggttc
    35041 ccagcatggt caggctaggg attttccgac cgtggaggac ttccttgtta agaatatcca
    35101 tacctgctcg gcccatttct tcagtataaa ctgtaatact agagagggga ggatagacct
    35161 gtttggtcag actagtgtcg ttaaaggaaa tgaggctgac gcgatctggc aggttgattc
    35221 tagcttcttg gagggcacgg agggcaccga tagctaaact atcgctggct gcgaaaaatg
    35281 ctggcggaag ttggtctccc aagctctgaa tggcttcctt catcaagtca tagccagact
    35341 gggcagtaaa gtttccttga aagatcagtt catcatgata gattcctttt gcttgactgt
    35401 agtttctgaa attttccagc cgcttatcct caatgatttc ttcttggtct gttgtttctt
    35461 caaggcctgt tagaatcccg atacggtcca ttccttggct gaggaaataa tcgacaacct
    35521 gttttatggc agtgtaaaag tccgtaataa tacaagtatg ccccagggaa agtgtatcac
    35581 tgtctagaaa gaccaggggt ttttggtatt cttcaaaggc agaaatctga gctcggctaa
    35641 attttccgat gcagagaatc ccaatcactt cctcgcttaa ggtaaaagga tggtcattaa
    35701 aatagcgcaa gatatcatag tccaactctt gggctctttt ttctattcct aggcgaatct
    35761 ggtagtagta gaggtcgtcc agctcccctt gttcgctgac ccattggata atggcaatct
    35821 tttgcttggg tttgtgggac tcgcctgtct tgaggtgctt ggtgtagccc agttcttcag
    35881 caacggttaa aatacggtgt ctggtttctt ctgtaacaga taggctctgg tcgcgattga
    35941 ggacgcggga tacggtcgcg atagagacag aggctagctg tgcaatgtct tttaaggtag
    36001 ccataaatcc tccttgatta ggttagtata tcatgttttt cttcttttta ctgatatttt
    36061 actaaaattt tagtaaaaag gattgacctt gagaaatccc ttggatacaa tagaagaaaa
    36121 cgattacacg ttaaggtggc ttaacggaca gtcaaaggag aaatcatatg acacaacatc
    36181 ttactgctga aactcttcgc aaagactttc ttgctgtttt tggtcaagaa gcagaccaaa
    36241 ctttcttttc accaggtcgt atcaatttga ttggtgaaca cacagactac aacggtgggc
    36301 acgtttttcc tgctgctatt tccttgggaa cttacggtgc agttcgtaag cgtgacgacc
    36361 aagtcttgcg tttctactca gctaactttg aggacaaggg cataatcgaa gtgcctctcg
    36421 ctgacctcaa gtttgaaaaa gagcacaact ggaccaacta tccaaaaggg gttcttcatt
    36481 tcttgcaaaa agctgggcat gtgattgaca aaggttttga tttttatgtt tatgggaata
    36541 tcccaaatgg ttctggcttg tcttcttcag catccttgga gctcttgaca ggagtcgtgg
    36601 cagagcatct ctttgattta aaactagacc gtttggattt ggttaaaatc ggaaaacaaa
    36661 cagaaaacaa ctttatcgga gtcaactctg gtatcatgga ccaatttgct attggtatgg
    36721 gggctgacca acgtgctatt tacttagata ccaatacttt agaatacgac ttggtgccac
    36781 ttgatttgaa ggacaatgtc gttgttatca tgaacaccaa caaacgccgt gaattggcgg
    36841 actctaaata caatgaacgt cgtgctgagt gtgaaaaagc agtggaagaa ttgcaagttg
    36901 ccttggatat tcagaccctg ggtgaattgg acgagtgggc cgttgaccaa tatagctatc
    36961 tgattaaaga tgaaaatcgt ttgaaacgtg ctcgccatgc tgtgcttgaa aaccaacgta
    37021 cccttaaagc tcaagcagcc cttcaagcag gtgatttgga aacatttggt cgtttgatga
    37081 atgcgtcaca cgtttctctg gagcgtgatt atgaagtaac tggtttggaa ttggatactc
    37141 ttgttcacac agcttgggca caagaaggag ttctcggtgc tcgtatgaca ggggctggtt
    37201 ttggtggctg tgccattgcc ttggttcaaa aagatactgt tgaggccttt aaggaagctg
    37261 taggcaaaca ctacgaggaa gtagttggat acgctccaag cttctatatc gctgaagttg
    37321 caggtggcac tcgcgtcctt gactagtcaa aaggaggctc tatagtgacc ttagtagata
    37381 aatttgtaac acatgtcatt tctgaaagtt catttgagga aatggatcga atctacctga
    37441 ccaatcgtgt tttggcacga gtgggagaag gtgttttgga agttgagacc aatctggata
    37501 aattgattga cctcaaggac cagctagttg aggaagccgt tcgattagag acgattgagg
    37561 atagtcagac tgcgcgtgaa atccttggta ctgaattgat ggacttggtg actccttgtc
    37621 cgagtcaggt caatcgtgac ttctgggaag cttatgccta ctctcctgag caagcgatag
    37681 aggattttta tcaactcagt cggaaaaatg actacatcaa actcaaggcc attgctaaaa
    37741 atatcgctta tcgtgttcca tctgattatg gagaacttga aattaccatc aatctctcta
    37801 agcctgaaaa ggatccgaaa gagattgcgg tagccaagtt ggtgcaagct agtaattatc
    37861 ctcagtgtca actttgttta gagaatgagg gttaccatgg tcgggtcaac cacccagctc
    37921 gcagcaacca ccggattatc cgttttgaaa tggttggtca ggaatggggg ttccagtatt
    37981 cgccctatgc ttactttaat gagcattgta tctttttaga tggccagcat cgtcccatgg
    38041 ccattagtcg tcagagtttt gaacgtctgt tggctatcgt agagcagttt ccaggatatt
    38101 ttgctggatc taatgccgac ctgccgattg tggggggctc tattctaact catgatcatt
    38161 atcagggagg ccgtcacgta tttcctatgg aattggctcc cttgcaaaag accttccgat
    38221 ttgctggttt tgagcaggtc aaggctggaa ttatcaagtg gccaatgtct gtcctacgct
    38281 tgacttcgga ttccaaagag gatttgatta acttagctga taagattttc caggaatggc
    38341 gtcagtattc agattctagt gtgcagattt tagcagagac agacgggaca ccgcatcaca
    38401 ccatcacacc aatagcgcgt aagcgtgatg gacagtttga gttggacttg gtcttgcgag
    38461 acaatcagac ttcagcagag catcctgatg gcatctatca tccccacaag gatgtccaac
    38521 atatcaagaa ggaaaatatc ggcttgattg aggtcatggg cttggcaatc ttgccaccac
    38581 gtctgaaaga agaagtggag caagtcgcta gctatcttgt aggagaagct gttacagttg
    38641 ccgattatca tcaggagtgg gcagaccaac tcaaatccca acatccagac ctaacggata
    38701 aagaaaaagc ccttgcaatc gtcaaggact ctgtgggtgc tatctttgtg cgtgtacttg
    38761 aggatgcagg agtctacaag cagacagaac aaggtcagac agcctttatg cgctttgtgg
    38821 aacaggtcgg aattttacta gactaggagc tttctccttg cacagtccta taaaaagtag
    38881 tatcatagtg tcgtttgaaa tatcaggagg aaacgatgaa agactttcat tttgacgcta
    38941 tatctgcctt tgaaaattac gaaattgaaa aaatgagaga tggtcacgtt gtggtgacga
    39001 ccaaagtagt gaactcgtcg ctcaactact atggcaatgc ccatggtggc tatctattca
    39061 cgctatgtga ccaaatcagt ggtttggtgg ttatctcgct gggacttgat ggggtgacac
    39121 tccaatcctc tatcaactac cttaaggcag gaaaactcga cgatgtattg accattaaag
    39181 gagaatgtgt ccatcaaggt cgtacaacct gtgtgatgga tgtggatatc accaatcaag
    39241 agggcagaaa tgtctgtaaa gcaaccttta ccatgtttgt cacaggccag cggtcagaag
    39301 aaagacaggt aaggatataa agagtaattt aatagagtga ggtgaatttt ggaatttata
    39361 agatttgcct tactcatttt ttgatattga tatgatttaa ttctaaaata gaaaatttag
    39421 taatgtggta gaaaatacaa gagttgtgtt ttaatttcta tggtataatt aaaagcatga
    39481 agataaaaga aataaagaaa gttactttac aaccgttcac gaaatggaca ggtggtaaaa
    39541 gacaattatt gcctgttatt agagaattaa tgcctaaaac ctataacagg tatttcgaac
    39601 cttttgttgg aggtggagct ttattttttg atttggctcc taaagatgca gttattaatg
    39661 attttaacgc tgaactaata aattgctatc aacaaattaa ggacaatcct caagaattga
    39721 ttgaaatttt gaaagttcat caggaatata attcaaaaga atattattta gatttacgtt
    39781 ctgcagatcg tgatgaaaga atagatatga tgtccgaagt acaaagagct gcacgtattc
    39841 tatatatgtt gagagtgaac tttaatggtc tatatcgtgt gaattctaag aatcaattta
    39901 atgttccata tggacgttat aagaatccta aaattgttga tgaggaattg atatctgcta
    39961 tttcagttta tataaataac aatcaactag aaattaaagt gggagatttt gaaaaggcaa
    40021 ttgtagatgt tcgaacagga gattttgtgt attttgaccc tccatatatt ccattgtctg
    40081 agacgagtgc atttacgtct tatactcatg agggattctc ttttgcagat caagtaagat
    40141 taagagatgc ctttaagaga ttgagtgata caggagctta tgttatgtta tcaaattctt
    40201 ctagtgcttt agtagaggag ttgtataagg attttaatat acattatgtt gaagctaccc
    40261 gaactaatgg agcaaaatct tcaagtcgag gaaaaatttc tgaaattata gtcacaaatt
    40321 atgaaaaata acgaatataa gtatggaggt gttcttatga caaaaccata ctacaataaa
    40381 aataagatga ttcttgttca ttcagatacg ttcaagttct tatcaaaaat gaaaccagaa
    40441 agtatggata tgatttttgc tgatccacct tattttttaa gtaatggtgg aatatctaat
    40501 tctgggggac aagttgtttc tgttgataaa ggagattggg ataaaatttc ttcattcgaa
    40561 gaaaaacatg agtttaatcg taaatggatt cgcctagcaa aagaagttct gaagcctaat
    40621 gggacggtat ggatttcagg tagtttgcac aacatatact cagttggaat ggcattagaa
    40681 caagaaggtt ttaaaattct gaataatatt acttggcaga aaacaaaccc tgcccccaat
    40741 ttatcttgtc gttattttac ccattctact gaaaccattt tatgggccag aaaaaatgat
    40801 aaaaaagctc gtcattacta caattatgat ttaatgaaag aattgaatga tggaaaacaa
    40861 atgaaagatg tctggaccgg ttctttaaca aagaaagttg aaaaatgggc tgggaaacat
    40921 ccaactcaaa aaccagagta tttgttagaa cgcattattt tagcctctac taaagagggt
    40981 gactatattc tagacccatt tgttggtagt ggcactacgg gtgttgttgc gaagcggtta
    41041 ggtagaagat ttataggtat cgatgctgaa aaagaatatt taaaaattgc aaggaagagg
    41101 ttggaggcag aaaatgaaac aaactagaaa ttttgatgag tggttgtcaa cgatgaccga
    41161 cacagtagct gactggactt attatacaga ttttcctaag gtttataaaa atgtatctag
    41221 tataaaagtt gctctaaata ttatgaatag tcttattggt agtaaaaata tacaagagga
    41281 ttttttagac ttatatcaaa attatccaga aattttgaaa gttgtgcctc tacttattgc
    41341 taaacgtttg agagatacga ttattgttaa ggatccgata aaagatttct attttgattt
    41401 tagtaaacga aactatagta ttgaagaata tactatgttt ttggaaaaat caggtatatt
    41461 tgatttattg caaaatcatt tagtatcaaa tttagttgac tacgttacag gtgttgaggt
    41521 tggtatggat actaatgggc gcaaaaatcg gactggagat gcgatggaaa atattgttca
    41581 aagctatctt gaagctgaag ggtatatttt aggagagaat ttatttaaag agattgaaca
    41641 aaatgaaata gaggaaattt tttctgtcga tttatctgct attacgaatg atgggaatac
    41701 tgtgaaacgt tttgattttg tcataaaaaa tgaacaagtg ctttatctga ttgaggttaa
    41761 tttttattca ggaagtggtt caaaattaaa tgagacggct agatcttaca aaatgattgc
    41821 tgaagaaatt aaagctattc ctaatgttga gtttatgtgg attactgatg gacaaggttg
    41881 gtataaggct aagaataatt tacgagaaac atttgatatt ttaccattct tatataatat
    41941 taacgatttg gaacataata ttttaaagaa tttgaaatag gtccaattca attagatggg
    42001 aattgggatt gattgtattt ataatcaatt tgtgataaac aaaaaagagg ctcgcacctc
    42061 tttttcttat ttctttttat gatttaatac ggcattgagg acaatggcga gtaggctggc
    42121 tacgacgatt ccgtttgaga agaacatttg gaaggctgtc ggcatgctga caaagagatt
    42181 actgttgttg agaccgacac ctgcagcgat tgaaacagct gcgataagga agttgtgttc
    42241 attgttagca aagtcaactc gggctaggat ttgcatccct tgaatagata caaaaccaaa
    42301 catcaccagc atggcaccgc cgaggacgga gcttggaatg atttgggcaa gggcaccaaa
    42361 cttaggaagc agtccaagga gaaccaggaa accagctgcg tagtagattg gcaggcgttt
    42421 tttgatgcct gacaatttaa ccaaaccaac gttttgtgaa aatccggtgt aagggaaggt
    42481 gttaaagatt cctccgagaa gtacggccaa accttctgcg cggtaaccgt tgcgaaggcg
    42541 cgtgctgtcg attggatctt ttgtgatatc agacaaggct agataaacac cagttgactc
    42601 aaccatagac accgttgcga tgatacacat catgacaata gatgagattt caaaggttgg
    42661 catcccaaag tagagtggaa ttgggacatg gacaagtgga gctaccgcaa caggagagaa
    42721 gtccaccaag cccatagtag cagcaatggc agttccaaca accagaccaa tcaaaataga
    42781 gatagacttg ataaatcctt tggtaaagat gttgatcaag aggataatca gaacagtaat
    42841 agctgcaagc aagagacttt gaccagttgg ctctggaacg ttatttccca tatttccaat
    42901 agcgacaggg atcaaggtta aaccaatcgt ggtaataaca gatcctgtta cgatagatgg
    42961 gaagagattg gctacttttg agaagatgcc tgaaacaaga accacgtaaa tcccagatgc
    43021 gataagggca ccaaacatag cgccactacc atggctttgc ccaatcataa tcaagggagc
    43081 gaccgactgg aatgcaactc caagaacgac tgggagtcca atcccaaagt atttgttgag
    43141 ttggagttgg aggaaggttg ccaccccaca catgaagata tctgtagaaa tcaggtaggt
    43201 caactgctca gctgaatagc caagggctgt cgcaatcatg atgggaacca ggatagatcc
    43261 tgagtacatg gctagtaagt gctgcaagcc cagaacggct gcttgcgagt gtttttcttg
    43321 agtttgcatt agagatctgc ctccttaaat acgacctgac cattttcaaa acgatccaag
    43381 cgagcaagtg ataggacagg gtagcctgct ttttcaagca aatcacgacc atcttggaag
    43441 gatttctcaa tcacgatacc gatagcttgg actgtagcac cggcctgttc gatgatttga
    43501 atcaagcctt tagcagcttg gccattagca aggaaatcgt cgataatcaa aaccttgtcc
    43561 tctggtgaga ggaattttcc agcgatagaa acggtgctgg tcacctgctt ggtaaaggag
    43621 tagacttgag cagttaagat gccttcgttc atggtgatgt tcttagcttt tttggcgaaa
    43681 atcatgggaa cgtttaaggc ttcagctgta aaaacggctg gggcaatacc cgacgcttca
    43741 atggttacga ccttggtaat gccagtagca gcaaattttt ccgcaaaaac cttaccaatc
    43801 tctcgcatca agctaaagtc aacttggtgg gttaaaaagg aatctacctt gaggatgtta
    43861 tcgcccaaga tatgcccatc cttgaggatg cgctcttcta ataatttcat aagacctcct
    43921 aaagtctaaa agttaattta cttgttgttt aaatgtttct atagtgatcc cttttgctaa
    43981 tactatatat ttgataaaac tattacgagc gaagcgagtc ttatcaaata tttcccgatg
    44041 tagtggtatc atagacaata atcttgttat tgtctatgac gggatttttg agagtaaaat
    44101 agttcgggga actattttag cctaagccta gaaatgaaag agctaggggc tcaaaaatta
    44161 gggatgaaat tccctggatt cctgaaatta ttcacaggat aatttcacct cccgtccgca
    44221 ctaattaagg gaaatattaa aaatattcaa aaaagaccta cttaatctct aagtaagtcc
    44281 cctaaataga catggcaaaa acggccatat ctcactgctg acttacttat tgttaggtgt
    44341 tccggcacct tgtagaaacg tcgtgccaat tcacgacata aacaagtaaa acgatattca
    44401 attttaaata ggcttgagcc aatgttttta ttttacacta aataacttta gaaaccaact
    44461 attttgttag tgttttggtt taaaaaacga acaaacagaa gagagggtga acaaaaactc
    44521 cattgtaagc taacatttat actaaatgaa aatcaaagag caaactagga agctatccac
    44581 aacctcaaat cactgttttg aggttgtgga tagaattgac agagtcagta tcatatacct
    44641 acggtaaggc gacgttgatg tggcttgaag agattttcga agagtattag aagatttttc
    44701 catcataaaa ggcatactat caagttttta gacacctgac aatatgcctt tttctaactt
    44761 taaagacttt tcccaatttt tattattcta ctcgctaaat cttaaaaaat agccatttgg
    44821 atccaaaact gcaaattcat gaggatagat atagtgatca ctgacacgaa actttctctt
    44881 ggtcaaggga cgataaatag gatagtttgc cttcatcact ctttaataga gttttgaaac
    44941 atcctttatg ccaaaggaga gattgactcc acgaccaaag ggataggtca gttcagctag
    45001 ttgatccttt gttccctcct ctaacattag ttgacactct tcaagagaaa gagaaagttt
    45061 tcttctggac gttggtattc aatcctaaaa cccagtaaac cacagtagaa ggaccgggac
    45121 tgttcgatat tcgatacaag caactcggga atgaccgcat tgtagtccat atagaaaatc
    45181 cttacaagtc aatttccaag acaatcggtg tatggtcttg gcgagcacct gagtcaatca
    45241 tatcagattt agtgaccttg tcagcgatac ggttacttgt gagccagtag tcgattctcc
    45301 agcctgtatt gttgatttta gaagttttgc tgcgttgtgc ccaccaagtg tagcgttcag
    45361 gaacatcgcc atgaacatgg cggaaggtgt ctgtaaatcc agttgccaaa aggttggtaa
    45421 atccagcacg ttcctcgtca gtaaatccag gtgaacggcg gttgctagca ggatttgcaa
    45481 ggtcgatttc attgtgggct acgttgtagt caccggtcgc aaggactggt ttttctttgt
    45541 ctagttcagc caaatactca gcatatttgg catcccagac ttggcgttct tccaagcgtt
    45601 tgagaccgtc accagcgttt ggagtgtaaa cttgggttac gaaaaatgca tcaaattcta
    45661 gagtgatgat acgaccttcc aagtccatgg tagaaggggc accgatttct gggaagctga
    45721 tagtaggtgt aagttctttc ttataaagga acatggttcc agcatagcct ttacgggcag
    45781 gctcttggga agagcgccac gtgttttcgt agcctgggaa gagttcttct aaaatttcca
    45841 cgtgtttctt tgtaggtcct ttggcagaaa gcttggtttc ttggatagca atgatatcag
    45901 cattttcagc gaccaaggtt tgtaggactt cttgggacaa tttggcacga gctgagtcac
    45961 tagttagggc agcgtttagg gaatcaatat tccatgagat aagtttcata aagttacctt
    46021 tttcattcag agtatagatt ttattatacc aaaaaaagat ctatttcccc aacgtatggt
    46081 ttgaaaaatt actctctttc gtttataatt aagaatgatt ttatgaaagg gagtgaaaat
    46141 acatgaaatt ctactcttat gactatgtac tcagccaaat cggtcagcaa aatggtatca
    46201 tggttggctt tgggattgtt ctattagctg tgacagtttt ttttgctttc aaggcatacc
    46261 ataataaaaa gggaagcgaa tttcgtgagt tggtcatgat ttcagatctg gccttattta
    46321 gctctgcttt tggtcagcat cacgacttat caaaacaatc aagtttctaa caataaattt
    46381 caaacttcac ttcatttcat cgaggttgtt tccaaagatt tgggagtaga caagtcagaa
    46441 gtctatgtta atacttccac aaacacagat ggcgcactta tcaaggtggg agatcgctat
    46501 tatcgtgccc taaacggaag tgagccagac aagtacctgt tagagaaagt cgaattgtat
    46561 aagacagacg caattgaact ggtggatgtg aacaaatgac acttaattat atcgaaattt
    46621 taatcaaact ggtcttgact ctcaaatagc tcaacaacaa tgttcacttt gtgaaacgtt
    46681 tgattgatgg taagccaact ctccttatca aaaatgggaa tattgaccca gaagcctgtc
    46741 gttcagttgg tttgtctgca tcggatgtat ccctcaaact tcgtagccaa gggattttcc
    46801 agatgaagca agtcaaacga gctgtgcaag agcaaaatgg gcaactcatc gttgtgcaaa
    46861 tgggagatga aaatcctaag tatccagttg tgactgacgg tgtgattcaa gtagatgtct
    46921 tggaatcgat tggtcgtagc gaagagtggt tgcttgataa cctcagtaaa caagggcatg
    46981 acaatgtagc caatatcttt attgctgaat atgacaaggg tgctgttaca gtcgtaactt
    47041 atgaataaga aaaacctggg gtcttgtact cttcgaaaat ctcttcaaac cgcgtcaacg
    47101 tcgccttgcc gtatgtaggt tactgacttc gtcagttcta tctacaacct caaagcagtg
    47161 ctttgagcag cctgcggcta gtttcctagt ttgctctttg attttcattg agtattggcc
    47221 tcaggtttcc atttgcaatc agaaagggat tttatgtcca ttattcaaaa actttggtgg
    47281 tttttcaagt tagaaaaacg ccgttatcta gtcggaattg tggccctgat cttggtttcc
    47341 gtcctcaatc tcattcctcc tatggttatg gggcgggtca ttgatgccat cacatcgggg
    47401 caattaaccc agcaggacct ccttcttagc ctattttact tgctacttgc agcatttggt
    47461 atgtactatt tgcgctatgt gtggcgtatg tatatccttg ggacctctta ttgcttggga
    47521 cagatcatgc ggtctcgctt gtttaagcat ttcacaaaaa tgtcgtcagc cttttatcaa
    47581 acctatcgga cgggtgatct gatggcacac gcaaccaatg atatcaatgc cttgactcgt
    47641 ttagcaggtg gcggtgtcat gtctgcggtg gatgcctcta tcacggctct ggtgactttg
    47701 ttgaccatgc tctttagcat ctcatggcag atgactcttg ttgccattct ccccctacct
    47761 ttcatggcct atacgactag tcgcctaggg agaaagactc ataaggcctt tggcgaatcc
    47821 caagctgctt tttctgaact caataacaag gtacaggagt ccgtatcagg tatcaaagtg
    47881 accaagtctt tcggttatca ggcagacgag ttgaagtctt ttcaggcagt caatgaatta
    47941 accttccaaa agaacctgca aaccatgaaa tatgatagtc tctttgaccc tatggttctc
    48001 ttgtttgttg gttcgtccta tgtttta
//
LOCUS       DAVKWS010000008        45279 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKWS010000008 DAVKWS010000000
VERSION     DAVKWS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.008"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(146..1126)
                     /locus_tag="VW852_000517"
     CDS             complement(146..1126)
                     /locus_tag="VW852_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV0271705.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHISQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLVF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1442..3457)
                     /gene="recG"
                     /locus_tag="VW852_000518"
     CDS             complement(1442..3457)
                     /gene="recG"
                     /locus_tag="VW852_000518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV0271706.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3476..4579)
                     /locus_tag="VW852_000519"
     CDS             complement(3476..4579)
                     /locus_tag="VW852_000519"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV0271707.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4569..4937)
                     /locus_tag="VW852_000520"
     CDS             complement(4569..4937)
                     /locus_tag="VW852_000520"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV0271708.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(4976..6007)
                     /locus_tag="VW852_000521"
     CDS             complement(4976..6007)
                     /locus_tag="VW852_000521"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0271709.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEVFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6009..7040)
                     /locus_tag="VW852_000522"
     CDS             complement(6009..7040)
                     /locus_tag="VW852_000522"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0271710.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7121..9634)
                     /gene="secA"
                     /locus_tag="VW852_000523"
     CDS             complement(7121..9634)
                     /gene="secA"
                     /locus_tag="VW852_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV0271711.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9780..10067)
                     /locus_tag="VW852_000524"
     CDS             complement(9780..10067)
                     /locus_tag="VW852_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271712.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICWLSLWINLFITFAILPIFGN"
     gene            complement(10078..10728)
                     /locus_tag="VW852_000525"
     CDS             complement(10078..10728)
                     /locus_tag="VW852_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0271713.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10732..11373)
                     /locus_tag="VW852_000526"
     CDS             complement(10732..11373)
                     /locus_tag="VW852_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271714.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(11366..12157)
                     /locus_tag="VW852_000527"
     CDS             complement(11366..12157)
                     /locus_tag="VW852_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271715.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12342..12677)
                     /locus_tag="VW852_000528"
     CDS             complement(12342..12677)
                     /locus_tag="VW852_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271716.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASGWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12817..13152)
                     /locus_tag="VW852_000529"
     CDS             complement(12817..13152)
                     /locus_tag="VW852_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271717.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13240..14550)
                     /gene="der"
                     /locus_tag="VW852_000530"
     CDS             complement(13240..14550)
                     /gene="der"
                     /locus_tag="VW852_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV0271718.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14564..15277)
                     /locus_tag="VW852_000531"
     CDS             complement(14564..15277)
                     /locus_tag="VW852_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV0271719.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15274..16170)
                     /gene="dnaI"
                     /locus_tag="VW852_000532"
     CDS             complement(15274..16170)
                     /gene="dnaI"
                     /locus_tag="VW852_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV0271720.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16171..17340)
                     /locus_tag="VW852_000533"
     CDS             complement(16171..17340)
                     /locus_tag="VW852_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV0271721.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17341..17814)
                     /gene="nrdR"
                     /locus_tag="VW852_000534"
     CDS             complement(17341..17814)
                     /gene="nrdR"
                     /locus_tag="VW852_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV0271722.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17954..18319
                     /locus_tag="VW852_000535"
     CDS             17954..18319
                     /locus_tag="VW852_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0271723.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18324..19019
                     /locus_tag="VW852_000536"
     CDS             18324..19019
                     /locus_tag="VW852_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271724.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19036..19803
                     /locus_tag="VW852_000537"
     CDS             19036..19803
                     /locus_tag="VW852_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271725.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20156..21355)
                     /locus_tag="VW852_000538"
     CDS             complement(20156..21355)
                     /locus_tag="VW852_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271726.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21352..22245)
                     /locus_tag="VW852_000539"
     CDS             complement(21352..22245)
                     /locus_tag="VW852_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271727.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22368..22559
                     /locus_tag="VW852_000540"
     CDS             22368..22559
                     /locus_tag="VW852_000540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271728.1"
                     /translation="MKIKEQTRKPAVDCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYTYGKAKLTWFEEIFEEY"
     gene            22952..23140
                     /locus_tag="VW852_000541"
                     /pseudo
     CDS             22952..23140
                     /locus_tag="VW852_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23555..24172
                     /locus_tag="VW852_000542"
     CDS             23555..24172
                     /locus_tag="VW852_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV0271729.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24215..25102)
                     /locus_tag="VW852_000543"
     CDS             complement(24215..25102)
                     /locus_tag="VW852_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0271730.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25251..27134)
                     /locus_tag="VW852_000544"
     CDS             complement(25251..27134)
                     /locus_tag="VW852_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0271731.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            27511..28965
                     /locus_tag="VW852_000545"
     CDS             27511..28965
                     /locus_tag="VW852_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0271732.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGIYYELDYGRKTN"
     gene            28946..29911
                     /locus_tag="VW852_000546"
     CDS             28946..29911
                     /locus_tag="VW852_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0271733.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(29946..31220)
                     /locus_tag="VW852_000547"
     CDS             complement(29946..31220)
                     /locus_tag="VW852_000547"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV0271734.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNDQEYTVPYVTEEPSVVAAASYASKIIKRTGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31220..32392)
                     /locus_tag="VW852_000548"
     CDS             complement(31220..32392)
                     /locus_tag="VW852_000548"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV0271735.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(32523..32771)
                     /locus_tag="VW852_000549"
                     /pseudo
     CDS             complement(32523..32771)
                     /locus_tag="VW852_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32839..33463)
                     /locus_tag="VW852_000550"
                     /pseudo
     CDS             complement(32839..33463)
                     /locus_tag="VW852_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(33525..33974)
                     /locus_tag="VW852_000551"
     CDS             complement(33525..33974)
                     /locus_tag="VW852_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0271736.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(33976..34734)
                     /locus_tag="VW852_000552"
     CDS             complement(33976..34734)
                     /locus_tag="VW852_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0271737.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(34849..36828)
                     /gene="pknB"
                     /locus_tag="VW852_000553"
     CDS             complement(34849..36828)
                     /gene="pknB"
                     /locus_tag="VW852_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV0271738.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(36825..37565)
                     /locus_tag="VW852_000554"
     CDS             complement(36825..37565)
                     /locus_tag="VW852_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV0271739.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(37580..38893)
                     /gene="rsmB"
                     /locus_tag="VW852_000555"
     CDS             complement(37580..38893)
                     /gene="rsmB"
                     /locus_tag="VW852_000555"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV0271740.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(38886..39821)
                     /locus_tag="VW852_000556"
     CDS             complement(38886..39821)
                     /locus_tag="VW852_000556"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV0271741.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(39834..42230)
                     /locus_tag="VW852_000557"
     CDS             complement(39834..42230)
                     /locus_tag="VW852_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV0271742.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(42296..42610)
                     /locus_tag="VW852_000558"
     CDS             complement(42296..42610)
                     /locus_tag="VW852_000558"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV0271743.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(42635..43261)
                     /gene="gmk"
                     /locus_tag="VW852_000559"
     CDS             complement(42635..43261)
                     /gene="gmk"
                     /locus_tag="VW852_000559"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV0271744.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLVAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(43391..45004)
                     /locus_tag="VW852_000560"
     CDS             complement(43391..45004)
                     /locus_tag="VW852_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV0271745.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT        12906 a        10340 c         8685 g        13348 t
ORIGIN      
        1 cgatttgact ctcctgatcg atttgtcctg ttcttgtttc attttactat atttccatga
       61 taaaacgcat agtatcaagt tttcttaatc ccctgatact atgcgtgttt gtaattttta
      121 agattttgtg cttagagtcg actccttatt ttagatattt aaaaggaatc tcacttccac
      181 agagccagtt gtagacttgg tcattgacaa atacattcat ggcttcgtga gcatactcag
      241 gcatgatgcg ataggtttta tcgcaggtca gacgattata aatcgcaaac tgggtaatgg
      301 gatagcaaac atcgtcgtcc aagcccgtaa tcatcttaac ctcaccttgg atacgatggg
      361 caagattttt gacatcgata taggcaaggg tcgccatgat ttcctcctct gtttcatgga
      421 aggggtcgtg aaacttgaaa taacggaaaa gttcgtcgta agcctcgcta gtattaccaa
      481 tctcaatcac ccgtctgaag tctgacaaga agggataaat ggcaactgtt ttctgaattc
      541 gaggattgag cgctgctgca actagagcta gagcccctcc ttgtgaggca ccatagctag
      601 aaagacgctt ctcatcaacc tgagacagac tagcaacaat ttcgaccaac tggtaaatat
      661 ccagataaac atccttataa aagaggtggt cccgaccttc cacagcacca cggataatat
      721 gccccttcac ggtatttcct aaaggagaac gcaagccgtc ttgtgagtaa cctgactggc
      781 cccgcacatc catggaaaca acaccgtaac cagctacggt gaagaccagc atgtcggccc
      841 agtcccagcc acgtcccata taaccatgaa aatggaagat tagtggaacc ttctcctcac
      901 tctttggaag aacaatgcgt gcatagacct ttccttcctt gcttccttca aatgttaact
      961 catagcactt gacttgagaa atgtggaaat ctctttcctc caagtggtag gatggaagtg
     1021 tggaaacatt tttcacttcc ccatcccaga aagcatcaaa gtcttccgga acctcatccc
     1081 ttcctctata ggtcttaatt tcttctagca aagctggatt tttcatagca tgctcctttt
     1141 attttgtaat cgttaccaca actgtagcat atctatgtaa tcaatgcaag aaagaaggat
     1201 aaatagaaag tgaatttaga ttctttactt ttaggatgat gggctgaaag tagttttaga
     1261 aacgaaaaag agcatttcgc tctctcttct gtttatacta gttttttgct tccttctttt
     1321 ggaaagtggt ttggtaattt actttaatcg ttactgtcat atgagagtcc tcgtttcttt
     1381 ttgatgactt tagtataaga gccaaaccta aaaactagct taagttttcc ttagagaaag
     1441 cttaatccag atgttctttc ttttccagat gaagggcaat catgcgccac tctggatctt
     1501 cttgccaagc ttctatagaa ctaatgtagc tagcaacctt tcttgcttct tctaaaatcg
     1561 gaaaatcttc gataatatca gccacttgga actctggaag tcctgactgt ctggttccaa
     1621 aaatctcacc agaaccacgc attttcaaat cttcctccgc aaggacaaat ccattggtcg
     1681 tttctgtcat gatgcgcatg cggtctttcc cagaatccgt cttgggattg gcaacgagaa
     1741 cagcgtagga ctgcttgtcc ccccgaccga cacgacctct aagctggtga agttgactga
     1801 gaccgaagcg atcggcatcc atgataatca tgacagtcgc attgggaacg ttgaccccaa
     1861 cctcaataac cgtcgtcgaa accagaatat ccgtctttct ctccttgaaa tcctgcatga
     1921 tctggtcttt ttcgtcactc ttcatcctac catgtagaag agccacctct gccttgcctg
     1981 caaaatgagt cgtcaactcc tctgataagg caatggcatt tttcaaatct agagcttctg
     2041 attcttcaat caaaggagag atgacatagg cttgggaacc tttttgaatt tccccctcta
     2101 accaagtcaa gacctgaggt agttgctcat gtttgatcca gcgcgtcaca ataggcttcc
     2161 gacctgctgg catctggtcg ataatggaaa catccatatc tccaaaggct gtgatggcaa
     2221 gcgtccgtgg aatgggagtc gccgtcatca tgaggacatc tggattgtca cctttttccc
     2281 gtaaaatacg cctttgccct acaccaaaac ggtgctgctc atcgataata atcaaaccaa
     2341 gacgagcata ctccacccca tcttgtatca gagcgtgagt tcctataatc aaatcagcct
     2401 cacccttggc aatggtctcc aagacctctc tcttttctgc agctttcaag gaacctgtca
     2461 agagagccag tttcaaattg ggaaaaaggt tctgtaaact ctcaaagtgt tgctctgcga
     2521 ggatttctgt tggtaccatt agggcagcct gataacctgc tgtcactgcc gcaaacatgg
     2581 ccaagccagc gactaccgtt tttccactcc ccacatcccc ttgtaggaga cgattcatgt
     2641 ggtggtcgga cttcatatca gttaaaattt cctgcaaact cttttcctga gcttgggtca
     2701 gggcaaaagg aagacttact ttaactgctg tcactttttc ctgagaccaa ttcagaacca
     2761 gaccacttcc ctgaactcta ttttcagact tgagcgtctg caactgcatt tggaaataaa
     2821 agagttcctc aaacttgata cggcgaagag cctgcttgta ttctgccaaa tcctttggaa
     2881 aatgcatagc tcggactgcc tgacaacggg acatgagttt gtatttgtct agtaaagact
     2941 ggggcagatt ttcttctatc aagaggtcca gtccctgatc aaaagccgtc ttgatgacct
     3001 taaccagact ggcctgactg attccctgag ccagacgata gacaggctgg aggtcatctt
     3061 ctacctgagc cagaaccttc atcccagtca gactagcctt agcgcggtcc cattttccaa
     3121 agacagcaag ggttgctccc aactctattt tatcagccag atagggctgg ttaaagaaat
     3181 tcaccgcaaa aacgacctct ccctgcttga gactaaaacg caggcgattg cgcttgaaac
     3241 cataatactg gacactagca ggagtcacta cctgaccaga aagaactgcc ttctcaccgt
     3301 cttccagctc cagcacctgc ttggttttga agtcttcata acggaaagga aagtagagca
     3361 agagatcttg caagttttca attcctagtt tggcgtattt ttctgctgac tttggtccca
     3421 caccaggcaa gacatgcaag ggttgatgta gattcatgct ccactccttt cttttctaat
     3481 aatattctct cggaatacgg tcgctgagga ggcaaaccac ctcatagtta atggttacgc
     3541 ggtaggtcgc tacctgagtt gcagtgattt ccttatcccc attggagcca atcaaggtta
     3601 ccttggttcc gagtgggtaa ggcttaggca atcgaatagt gatttggtcc atcgaaaccc
     3661 tgccgacaat tgggcaagct tggccatcta ccaagacaga gaaattttgc atgtctcttg
     3721 tccatccatc tgcatacccg attggcacgg tcgcgatgac ttgctcgcta tccgcttgat
     3781 aagttgctcc atagcccatg caagctccag ctggaactgt cttgacatga accagagcag
     3841 actccaaggt caaggccggt atcaaatcat aaggcaaatc caagaccgct ccacttggat
     3901 tgaggccata catggcatct cccatacgaa ccgcattgaa aatagtctct acatgccaaa
     3961 gagtcgttgc agaattgcta gcatgaacca gctctggaac ttccttcata ctagctaaaa
     4021 tagttttaaa ccgttctaac tgggcattaa aatagtcatc tgattcctca tcagcagtag
     4081 caaagtgggt aaagattcct tcaacacaaa caccgtgttg ttggagcaaa tcttgagcct
     4141 gctcaacctc acttgcctct ctaaaaccaa tccgtcccat ccctgaatca atcttgaggt
     4201 ggactgtcaa tccagttagg tccacttcct tatctaagag tgcttgaatc cactccagtc
     4261 cagccactgt caaggtgaag tcatattctt tagctagagc aacagcttcg atttcagaaa
     4321 ctcctaaaat gaggattggc ttgctgagtc cagcttgtct gagttcaatg gcttcatcga
     4381 tattggaaac gcaaaagcca tcaacatcat cttgaattgc cttggcaacg gcaacagctc
     4441 catgaccata agcattggcc ttgaccacag ccaacttgag cgttccttga gggatatgag
     4501 cccccatttg ctgaatattt tgtcgaatag ctcccagatg aatcagagcc ttggttggtc
     4561 tatgtggact agctttcatg attttcctcc aaaatgacac tggctgtcac aaactgatcg
     4621 gtgtggctga tagacagcca aatctttcct gaaaatggtg cctgactaaa ataaggcgcc
     4681 ccacgttcat tgttcaagac ttccaaatcc tgaaaaccga gcttgctaat gcccgttccc
     4741 atagccttgg aaaaggcctc cttagccgac cagcgaccag ctaaatattc tatttgcctg
     4801 cgtcctttga gactggtgaa gcgctccatt tcctgagcgg tcagtacacg cttagcaaat
     4861 ccttcatgtc gtgtaactgc gctttctatc gaagccaatt cttcgatgtc aattccgtgt
     4921 ccaactatca ttctcatcaa aaggagttga gattccccaa ctccatcctt ttcacttatt
     4981 ttgtcaaggt aacataaatc tcttctacca aggcctctgt attttcccaa cctaggcaag
     5041 ggtcagtaat agagcaacca aagacctctg gttggttttg acgaccatct gctaggtaag
     5101 attcaatcat aaatcctcga accgtctttt taattttctc attccaatca cgattctgca
     5161 aggtctggcg aacaattcga atctgctcca tatattgctt gcctgagtta tcatggttgg
     5221 tgtcaatgag gataaaagga ttttcaagtc ccatggtttc atagcgttca atggcttgga
     5281 gtaggttttc atagtagtaa ttcggcatat aattgccata ctcgttgact gctccacgga
     5341 ggataacatg ggccaaagga ttacctgatg tctcaacttc ctgcccatga taaaggaagg
     5401 tttgcttgtt ttgagcagca tagatggcgt taaacataac acccaaattt cctgaggttg
     5461 gatttttcat ccctactggt gcatcaatcc cagaagccac aaagcggtgc tcttggtctt
     5521 ccacagaacg agctccaacg gcatggtagc tgaccaagtc atccaccaag atcagatttg
     5581 acggataaag catctcatct gccgttgtca aaccagtctc tgtaatcacg cggtagtgca
     5641 actggcgcac agcctgcaag ccgttaatca ggcttggagc cttagaagta tctggctggt
     5701 gaactaatcc tttatagccg tctccattgg tacgaggctt agcagtatac acgcgcatga
     5761 ccatgaaaat cttatccgct accttctttt gcaaggcgga taaacggcga gcatattcca
     5821 agaccgcctc ttcattatca gaagagcaag gaccaatcac caagagaata cggtcatctt
     5881 cccctgaaat aatatctgcc aattccctgt cacggctttc cttgagtctc aaggcttccg
     5941 cagacaattg ggtttcagcc ttgatgactt ccatatcgat ttcttgacct ttttcaataa
     6001 atgccatctt attctcctaa cgttttgtaa atttctctga caagagcttc tgtgttatcc
     6061 caacccaggc aagggtctgt gatagactta ccaaatactt ctggctcatt ttgtcgacca
     6121 tcttccagat aagactcaat cataaaacca cgaacgaact gcttaatttt ttcattccaa
     6181 gcacggttaa tcaaggtctg gcggacaatt cggatctgtt caatatactg cttaccagaa
     6241 ttgtcatgat tggtatcaat gatgataaaa ggattttcca aacccatttt ctcatactgg
     6301 gcaatggtat caattaaatt gtcataatag tagttgggaa tattttttcc atactcatta
     6361 agagcaccac gaagaatagc gtgtgaaagc gggttcccag ttgtttctac ttcttttcct
     6421 aagaaaagga aactttgttt gttttgagca gcataaatcc cattaaacat gacattgaga
     6481 tttccagagg ttggattttt aaaaccagta gaaaatcctg ccccacttgc cacaaagcgg
     6541 tgttgctggt cttcaactga acgggcacca actgccatgt aagaaatcaa atcatctaca
     6601 agcggaaggt tttcaggata aagcatttca tcagcagttg tcatccctgt ttctgtgatg
     6661 acacgatagt gaagatggcg aacggctttg attccattga taagactagg cgcttctgtc
     6721 gcgttaggct ggtgaatcaa gcccttatag ccatctccgt tggtacgggg tttggcagta
     6781 taaacacgca taaccataaa gatacgatct gccacttctt cttgtaggac tgccaaacgc
     6841 ttagcgtatt caaggacagc ttcttcgttg tcagatgagc atggcccgat taccaagaga
     6901 attcgctggt cttctccacg tataatggct tctagctctt gatctcgctg tgatttcctc
     6961 tccaaagcct gaccttctaa ttttgacaag gcacgaactt cttctatatt tattttagag
     7021 ctttttgctg taaataccat aactcatctc cttataaagc aaacggatac ccagtaaaaa
     7081 tttacttttc acaagatatc cgcctctaaa ctatctcatt ttattgtctt ttaccgtgac
     7141 agtttttaaa tttcttacca gaaccacatg ggcaaagttc attgcgtcca atctggctca
     7201 aatccaaatc ttctggcata cttgcttggt gagcagcgat attgcgagtc gctgttgtac
     7261 tgatatgacg ttctgcctgt ggtctttctt gttcatgaat ttgtgctttc atcatcaagc
     7321 gtgtcacatc aaactcaatc gaaccaatca tatcattaaa catacggaaa ccttctgcct
     7381 gatactcaac aacagggttg ttctgagcat agccacgaag tccaaccgcg ttacgcaatt
     7441 gatcaagggc atcgatatga tctgtccact tgttatccac cactcgtaga atcaaaactt
     7501 tttggaattc tttaactgct tcttcatcgc gtagttttga aacctgacta tcgtaaacct
     7561 tcaaggcacg ttggaaaagc tcttccttga tggccttatc agacaagcct gacaagtctt
     7621 ccatcgtaat agaatcttca ggaagcaagt tgtacttagc gaagttcaaa attgcctcta
     7681 gtttctcatc ttgtttagca cgcgcatgac cgtcaacgac acgaccaatc gtgcgtttga
     7741 tcattgcctg aatttcaggt gccaagtcac ggtctgcagt aatgacatca taacgttgag
     7801 tatagataat ctcacgttgt tcacgcatga catcgtcgta ttgaaggact tgtttacggg
     7861 tatcgtagtt atttccttcg acacgttttt gcgctgcttc aacctgacgc gtcaacatgc
     7921 gagactcaat cgcctcttca gacatgttga gacgttcaaa gattcccttc aagcgttcag
     7981 aaccaaaacg tttcatcaaa tcatcttcaa gagataggta gaattgtgac tcacctggat
     8041 ctccttgacg acctgaacgt ccacgaagct ggttatcgat acgacgactt tcatgacgtt
     8101 ctgtaccaat aacacaaagt cctccaagtt cacgaacacc ttcaccaagc ttgatgtcgg
     8161 taccacgacc cgccatgttg gttgcgatgg taacggcacc acgttgacca gcattcatga
     8221 tgatttgggc ttctctatag tggtttttgg cattcaagac ttcgtgagga acaccagctg
     8281 caaccaattt cttagaaatg tagtcactag tttcaaccgc tactgtacca accaagacag
     8341 gttgaccctt ttggtaacga gccttaacgt cttcgacaac cgctttaaac ttagattcga
     8401 tacttgcata aagaaggtct gagtggtcaa tacgttgaac aggacggttt gttgggattg
     8461 gaataacacg aatgttgtag atttcacgaa attcttcttc ctcagtctta cctgtacccg
     8521 tcataccaga caattttttg tacatacgga aaaggttttg gtacgtgatt gaggcagatg
     8581 tcttggtttc atcctggatt ggcacacctt ctttggcttc aatagcttgg tgcaatccat
     8641 cagaataacg acgaccttcc atggtacgac ctgtaaattg gtcgacaatc aagatttctt
     8701 gctcttcgct caccacatag tcaatatcga gaagcatgat gtagttggca cgaagggcgt
     8761 tatcgataaa gtgggtcaaa gccacgtttt cgatgtcata gaggttttca agtttgaagt
     8821 agctttcagc cctgtcaatc cctgaatcag acaaaccaat agtcttagac tgcacatcga
     8881 tgatgtagtc atctttgttc aaagatttta catagtggtc tgccatgtga tacaactgac
     8941 tggtttcaac cgcattggca cctgatacaa tcaaaggtgt acgagcctcg tcaatcaaga
     9001 tagagtcaac ctcatcgacc aaggcatagt taagcggacg ttgtaccatg ttttcagcgc
     9061 gaacaaccat attgtcacga aggtagtcaa atccgatttc tgagttagtt gagtaagtaa
     9121 tatcacactc ataggcttct tttttctcca ttggagattt ggtagccaag ttaatcccta
     9181 ctgacaaacc aagccaagag tacaattcac ccatctcagt cgcgtcacgt tctgacaagt
     9241 attcattaac cgtaactacg tgaacccctt tacctgaaag ggcattgagg tataccggca
     9301 tggtcgcagt caaggttttc ccttcccctg tacgcatctc tggcacgtca ccatggtgaa
     9361 gaacaatccc ccccatgacc tgaaccttat aagggaagag acctaggaca cgtttggcac
     9421 cctcacggac aaccgcaaat gcttcataaa gcaatgaatc cagtgattct ccattttgat
     9481 aacgttcttt aaattcaact gttttcgctt ttaattggtc atcagtcaaa gcagccattt
     9541 ggtcttcata tttgaaaacc ttgtcagcca tcttttccag acgacggatt tctcctttat
     9601 cattttcgat aattgttttt aaaatattag ccatgttttt ccttacttta aattccgaat
     9661 attttagaat gttcctttaa ttttagcaca attactgatt attttcaagg aagaatcccc
     9721 attttgaaaa ggaaaaagag gttagttttc aagctaacct ctccgacttt tatgactgtt
     9781 taattgccaa aaatcggcaa aatggcaaat gtgataaata agttaatcca aagagagagc
     9841 cagcaaatca gtccaaaaac aaagagactg actttcttgg acagcaaggc catcaaaatg
     9901 gacagaattc caaaaactag caagactttc tgtatgatga agatgtcaat ccttaccccc
     9961 agaatcgctc tatcccaagg gataaagaag aaagacagcc agatcagcag aactaaacca
    10021 atataaacct tggaatagcg tgtaataaat gattttagat gtgccataag ccatctccta
    10081 ggattgtata tcctttacat attgtatatg tccattttcg acaactaata catctgtaca
    10141 aaggtctgaa atatcttccg atatgtgcga cgtcaggata accagaccat tttctttttt
    10201 caggtaagat aaaatgacct gtttggttaa cctcacactt ttctcatcca aagcattcat
    10261 aggttcatcg agaaagagta tagaaggaga ggaaataaag gcttgaatca aggccatttt
    10321 ttgctttgtt cccaaggata aatgacggta ttcaacatct tcaaattcct gtaaatcata
    10381 gtattgaatc caataggcaa ttcttttttc cgtaacttta gatgaaaaat accttaacag
    10441 ttccaaattt tctctcaggg ataaatgaga taaaaactcg actttttcaa ttaaaattcc
    10501 tgcatcctga atataatgat tttttacccc gtaaacttta ccatcttgaa gaacttttcc
    10561 tttgtcaagc ttaatataac cagcaagtat ctttaaaaga accgtcttgc cagatccatt
    10621 atcaccttta agtccataaa tttttcctga ttgaaacacc aaattcaact gatttaaaat
    10681 aggtcttttc ccataaattt tctgtccatt tataatttca agtctcatcg tttatccttt
    10741 cttaaatttc tcttccaagc gactaagaat caacataact aataagctta agagtactaa
    10801 atacactgtt acaatatgat ccatcatcca ttcttgtccc acaaatgcta aaaaatagag
    10861 aaaaaaggaa aaaccagcgc ccgcactaat cagtgctatc agataagaca gtagtaagta
    10921 caacatcatt aactgagcaa gtaaaaagaa aagtgcgact gttttgaaat gaattcctag
    10981 aggaagtaaa ccaagaaacc agacaaacaa atcttgagta aaaaatttag aaatgacaac
    11041 caatcgtttc cttgtataat cgaaaaagga gcttgattga tagcgaatca agtgatggta
    11101 atggtctgcc aaatcacttt caagtaaaat gacagagagc catcctacca tgaaaagcga
    11161 aaggtttaac aagagtttgg ggatttctaa attcgcttca gaatagtcat tataggaaaa
    11221 aattgaaatc aattctctct tgaaaaagat aacagcatcc tgcttttgaa ccgctgttga
    11281 caatagatgg tttatcaaaa tcatcatcag aactatacga agtagtaaaa caatctttct
    11341 ctttagccaa atattgttaa aattcttaaa cattatcttc atatttttca agcaccatgt
    11401 aagggacaat ataaaggata taaggagtaa agagtttcat caaacttaca tccccatagc
    11461 tagcttgcat caaactcgtc ataggaacca tatagtaagg caaggcacta tagagcagca
    11521 tagaaccaag ccaaagaaac atcaaatagg tgattgccga acgagtcaca ttccccacat
    11581 aatcaactat ttgtaaaaaa tagattgcat tgatgaaaat accgattagt aggacacaag
    11641 taaagaacaa atagcttttt atctctccat ctaggagtct ttgtaaacca cttccatcag
    11701 aaaatagaga attccatcca agaggagaaa aagtcccaaa gaaataggtt ataattgcaa
    11761 ttatcagcac agtcactaaa tatatcaaac aagggatact tgcaacttgc aaagtcaact
    11821 ttctttttaa cccttgataa ctcacttctc ttccaatact caatcgtaaa actttatttt
    11881 tcagctcaat atattgaaaa cctagcaaaa caatgatgat tggaataata aatgattgat
    11941 aaaaacctag attgaactgg agaatacgaa aatctaatgc tgattttaat aagtcatttt
    12001 taattgcttg tccatcgaat ttagctagat cgggcgcatg aatatcaaaa aagaaataag
    12061 tcatcaaaac atcacgataa accaaagcaa gaaaaagcaa agtaagcaag acataaacaa
    12121 atttatgcct tgcaaaagtt aagagaggaa tcttcataag ccacctccta caaatagaaa
    12181 gtaatataat actcaatgaa aatcaaagag caaactagga aactagccgc aagttgctca
    12241 aagcactgct ttgaggttgt agataagact gacgaagtca gttacatata taatccaagg
    12301 cgacgttgac gtggtttgaa gagattttcg aagagtataa atcaatgcct tccgccttta
    12361 gacttcccta gttcccgtct caagtcaagc atttttttga aacagatata gtctaaccag
    12421 ccactagcaa aagcgataag tagtaataat agccaaatac cccataactt gatatctgtc
    12481 aaatttctca agacgatatt gaaaaacaga actgaaaaaa ctgtccaagc aaggctaaaa
    12541 agagcataga gggtgatgta aatccagtaa aaatagtaaa aaattgggaa aatcttacta
    12601 tctctgtttg ccttttcaat ccagctgaaa aagtaaaaac agggtaataa gattatcatt
    12661 ttaaacaagt attccatcac aaacctcctc ttttaaattg ttttctttag tttcgttcta
    12721 ttaaatgtta ttaccaatat aaaccttgga atagcgtgta ataaatgatt ttagaggtac
    12781 cataagccac ctcctacaaa tagaaaccga tataaatcaa tgccttccac ccttagactt
    12841 ccctagttcc tgtctcaagc gaaacatttc tttgaaacag gaataagtta accaattcat
    12901 accaatagct agcagaataa aaagaaacca aatgccccat aacttgatat ctgtcacatt
    12961 tctcaagacg gtattgaaaa acagaactga aacaactgtc caagcaaggc taaaaagaga
    13021 atagaagggg atgtaaaacc agtaaaaata ataaaaaatt ggaaaaaact tactatttct
    13081 gttggccttt tcaatccagt tatcaaaata aaagtacggt gctaaaagta agaatttaaa
    13141 caaatgttcc atcaccgaca tcccccttct tttgatagcg ttttctatca ttttattata
    13201 tcaaaaaaat ccggaactgt cattccagat tctaattttt tatttgcgtt ttcttgcgat
    13261 gagatgaatc ggtgttccct caaaaacaaa ggccttgcgg atttgatttt ccaagaaacg
    13321 caggtaagaa aagtgcatga gttcttcttc attgacaaag atgacaaagg ttggtggttt
    13381 ggttgccact tgggtcgcat agaaaatctt gagacgtttt cctttgtctg tcggtgttgg
    13441 gttgatggca atggcatcca tgatgacatc gttcaagaca gctgatggaa tacgtgtatt
    13501 ttgactttcg ctgatttgct taatcatctc aggaagtttg tggagacgtt gcttggttaa
    13561 agctgataca aagataatcg gtgcgtaagg caggtattgg aactgctcac ggatatcttc
    13621 ttcccagttt ttcatagtgt ggttatcttt ttcaagcgta tcccacttgt tgaccacgat
    13681 aatcatccct ttaccagctt catgggcaaa tcctgcgata cgcttgtcgt actcacgaat
    13741 gccttcttcc gcattgatga ccatcaagac cacatctgaa cggtcaatag cacgcatggc
    13801 acgcataaca gagtatttct cagtattttc ataaacctta ccagacttac gcataccagc
    13861 cgtatcaatc atggtaaact cttgaccatc tgtatctgta aagtgggtat caatagcgtc
    13921 acgagttgtt ccagcaacag gactggcaat aacacggtct tctcccaaga tagcattaat
    13981 caagcttgat tttccaacgt taggacgacc aatcaagcta aacttaatga catctggatt
    14041 ttcttcttca tattcatttg gaaggttttc cacaatcgca tctagtacat cccctgtacc
    14101 gataccatgg acagatgaga taggcagtgg ttcacccaaa ccgagagcat agaaatcata
    14161 gatatcattt ctcatctcag ggttatccac cttgttgact gcgaggataa ctggtttgtg
    14221 ggtcttataa agcttgcggg ctacgtattc gtctgcatcg gtaattcctt ccttaccgga
    14281 cacgacaaag acgataacat ctgcttcttc catggcaatt tctgcctggt gcttgatttg
    14341 ttccatgaaa ggagcatcga catcatcaat tcctcctgta tcaatcatgc taaaagaacg
    14401 attgagccac tcacccgttg cataaatacg gtcacgtgtc actccttcga catcttctac
    14461 aatggagatt cgctcaccag cgatccgatt aaatagggtt gatttcccaa cattgggacg
    14521 tcctacaatg gcaatagttg gtagggccat aatttctcac tttctacaat aatttcttct
    14581 gttcaagatt ttttctagtt gagcttggtt cagcttgacc aaactgttct gctaggcgct
    14641 gactccagct tgtggtcgca cgcgccccag catagtcagc ctgaacacgg tcataagctt
    14701 ggattgcctc agttgactgt tcttggtatt cttcctcaaa gacaacattc tctagtggca
    14761 gtctcggttt catatcatga tgttgatttg gcacacccag tgccatccca aagacagaat
    14821 aggtgtagtc aggcaggtta aagagctctg ccacttcttc agacttgtat cgaaccaaac
    14881 cgataatcac accaccatag cccaagcttt cagctgccaa caaggcgttt tgtccagcaa
    14941 gagctgcatc gaccgaacta atcaagagac cttccacacc ttggggttgg aaggtgtcgg
    15001 tatgaagtcg ggctcccttt tctgctcggt tcaaatctcc gacaaagaga aggaaaacag
    15061 cagactggcg aatggcttct tgaggtacca attcatacaa ggcatctttc ttctcttgac
    15121 ttcgtaccac aatcacagag taggattgga aattcttcca agatgatgcc atctgggctg
    15181 ctgtcaaaat ctcatttaag tctacttggg gaatttcttg ctctttaaac ctgcgcactg
    15241 aagtatgagc cttcatcaat ttaatggttt ctgtcatcga cggtttactc cttctaaacg
    15301 agtctcctca gccaaataac ggatgcgttc catgacccgt ctggcttccc aggtttcgtc
    15361 atttccatgt ttcactttcg caaaatgctt ctccaaatct tcaaagttga agttggatgt
    15421 gaaaaaggtc ggtaaatttt cctgcatccg atattggaga atgacctgca ggatttcgtc
    15481 acgcacccaa acggttgatt gctcggcgcc aatatcatct aaaatcagga cctcagacag
    15541 cttaatctca tccaccaagg tcttaacatt gccatcactg atagcatttt tgacatcaat
    15601 gacaaagcta ggatagtgga ggagagttga tgaaacacca cgtttttctg ataaatcatg
    15661 agctaaggcc gccaccatga aacttttacc cacaccaaag tctccatata agtaaagacc
    15721 ttttcgaata gctggatatt gctccacgaa ggctagtagc ttttcaaaaa ctggtaagcg
    15781 ccccaaatca tccaagtcaa cttgagccaa actagctttc ttgagactgg ctggtagatt
    15841 gattaacttg agacggttct taatagccgc ttctttttca gccgcgatta gctcaggagt
    15901 ttcttcatat gaaacatctg catagccatg attcttaacc aaaatcggct tgtagccttt
    15961 ggcaatataa tccgtatccc cacggagaaa cttgtcacgc tcggtgatgt actgattaaa
    16021 cttggagata ctgcgattta attcctctgg agtcaaggat tcttgctgga taaaagccgc
    16081 aacatcaggg tccttcatga ttttctggac caaatcttga taataaaaac ggctgggttg
    16141 acgtttgagt acgtctccga cactttccat ctaatctcct cctttttcta atcgagctaa
    16201 tagttcttgc ttcttacgtt ctagttccag acgagtttcc tcgctggttt cattcttata
    16261 ttcaggatta ctccatttag gaacattggt ttttactggg gcagtctgat tctgtttttg
    16321 tgtttttgct ttctgtcctc gctcacggat gcgcaagact gccttttctg ccgaatgaat
    16381 cttttgatag gcatagtcat tggctacctt catggcatat ttctcattga tatttgccga
    16441 atcgacctta ttaaaggtca ataaaagaat gatattgatg acttcgtcca gcaagcccaa
    16501 gccagccatc tgttgcaaga gttctctttc tgtttgggta atggttccct tgcgtgtttg
    16561 cttgatttct gccaagaact gcagggcagt tttacttttg gcttctttga taatggttgt
    16621 ttccttaaga ctaaagtcag aggaaactgg tttttgagca attttttcac gcatgcgttt
    16681 ggttgaaata acctgggaaa cagctgttga cttggccaat tgataggttt caaaccaagt
    16741 ccatttcttc tcctcggcaa tagcaaagag gtttaagaca tcggactgct catccgcaaa
    16801 acgaagtcca tctcgagcca tcagctggcg aaaatgttcc aagtcaaaat cattggccac
    16861 tttcttcttg agaccaaggt cttcttgact gcctagttct gccaattctg gaaagacttg
    16921 attgagtgag acaggtattt cttcaccatc agcactttca actttcaaat cctccacagc
    16981 agagtcgcca atctttttct ctaagagtct gcgataaaca ggatgctcca agaagtcttg
    17041 actagataga ggagcatgga gggctagctg ataaacatca cccttttgat agagggtcaa
    17101 gagattaaaa gcagataaga ttttcaatga ttttatcagt ctatccatcc caaagttgag
    17161 atgattgagg atacttgaaa aaagatattc ctttctacca ttatcccaaa aactgattgt
    17221 ataaagataa aggctcagtg cctcctgacc gataatcggg aggtagcact gtaccagaga
    17281 tgaggtatct tgcgacaccc gattattctt tagataagaa aaacggtcaa ttggcttcat
    17341 ttatctttcc tttttctttt tagaggactg ggtgatttgt tggagcaagc tctctaactc
    17401 actgacatcc ttaaaactac gatagacact agcaaaacgt acataggtaa tctcgtccaa
    17461 ttcagccaac tcctccatga cgagtgaacc aatgtcctca ctttgaattt cattttcatt
    17521 tcgaccacgg agtttctgtt cgatacgatt gactaccatg ttgatttcat cacttgacac
    17581 aggacgtttc tgggctgagc ggataatccc attaaagatt ttatctctgg agaattgttc
    17641 ccgtgtgcca tcttttttaa caaccactaa ggttctttct tctactcgtt cgtaggttgt
    17701 aaaacggtgt tggcactcgt cgcactctcg tcttctacga atggtgttcc cttcttctgc
    17761 ttggcgacta tcgataacac ttgacttggt agccccacat tttggacaac gcatcctttc
    17821 cctccttatc gttttctttt cattatacca ttttttaagc gattcccaaa acaattcttc
    17881 tttttgcttg acaagttttt tgttttgttg tattatttaa ttaagacaaa aggataagag
    17941 aaaggagact aagatgtcct ggacatttga caacaaaaaa cccatctatt tacagattat
    18001 ggagaaaatc aagcttcaga ttgtttccca tacactggaa cccaatcaac aacttccaac
    18061 cgtgagggag ctagctagcg aggctggtgt caatcccaat accatccaaa gagccttatc
    18121 agaccttgaa cgagaaggat ttgtctacag caagcgaaca actggacgat ttgtgactaa
    18181 ggataaggag ctaatcgccc agtcacgcaa acaattatca gaagaagaat tggaacactt
    18241 cgtttcctcc atgacccatt ttggctatga aaaagaagaa ctaccaggcg tagtcagtga
    18301 ttatattaaa ggagtttaag cctatgtcat tactagtatt tgaaaatgta tccaaatcat
    18361 atggagcaac accagccctt gaaaatgttt ctcttgacat tccagctgga aaaattgtcg
    18421 gccttcttgg gccaaacggc tcaggaaaaa caaccctgat taaactaatt aatggcctct
    18481 tacaaccaga tcaaggacgt gtcctcatca acgacatgga cccaagccca gcaaccaagg
    18541 ccgttgtagc ttatttgcct gatacgacct atctcaatga gcaaatgaag gtcaaagaag
    18601 ccctaaccta cttcaagacc ttctataaag atttcaatct tgaacgcgcc catcatctac
    18661 ttgcagacct gggcattgat gaaaatagtc gtctcaagaa actatcaaaa ggaaacaaag
    18721 aaaaggttca actgattttg gttatgagcc gtgatgctcg tctctatgtt ttggacgaac
    18781 ccattggtgg ggtggatcca gcagcccgtg cttatatcct caataccatt atcaacaact
    18841 actcaccaac ttctaccgtt ttgatttcta cccacttgat ttctgatatc gagccaatct
    18901 tggatgaaat tgtcttccta aaagacggaa aagtcgtccg tcaaggaaat gtagatgata
    18961 ttcgctacga gtcaggtgaa tccattgacc aactcttccg tcaggaattt aaggcctaag
    19021 caaaggagat tatttatgtt ttggaattta gttcgctacg aatttaaaaa tgttaacaag
    19081 tggtatttag ccctctacgc agccgtgcta gtcctttctg ccctcatcgg aatacagaca
    19141 caaggcttta aaaatctacc ttaccaagaa agtcaggcta ctatgctact ttttctagct
    19201 acagtctttg gtggcttgat gcttacactt gggatttcaa ccattttctt gattattaaa
    19261 cgcttcaaag gtagtgtcta cgaccgacaa ggctatctga ctttgacctt gccagtttct
    19321 gaacaccata tcatcacagc caaactaatc ggtgccttta tctggtcatt gattagcacc
    19381 gctgtattgg ctctaagtgc tgttattatt ctggctttaa cagctccaga atggattcct
    19441 ctttcttatg tgattacatt tgtagaaaca catctccctc agatctttct tacaggtata
    19501 tccttcctac taaatactat ttcaggaatc ctctgcatct acctggctat ttccattgga
    19561 cagcttttca atgaataccg tacagcactc gctgttgcag tctacattgg tatccaaatc
    19621 gtcattggat ttattgaact tttcttcaat cttagttcta atttctatgt caattcactg
    19681 gtaggactca atgaccattt ctatatggga gcaggtatag ccattgttga agaactcata
    19741 ttcatagcta tcttttatct cggaacctac tacatcttga gaaataaggt taatttgctt
    19801 taaataattt ttacctagat atgtaacata ctcatagaac aaaagagacc aggcaaaaag
    19861 tctttacaat tagaaaacgc atagtatcaa gtgttaaaaa tccttgatat tatgcgtttt
    19921 ttgtgagctg acttatttcc tttcactata tcgcaaaatg aaataagaac ggaacgatgg
    19981 gattttggaa ttcaaatcaa tttataagaa tgttttagaa gtaatattat cctattccag
    20041 attcagttca ctatacaatt gagttttcaa gcaacctgtt tacataatgt gtacataatt
    20101 aggttcgtga ttccaccctt ttcaccttta aaaacctcgc tttcgcaagg ctcttctatt
    20161 tataagataa ggcacgttta aaggttttcc aaatccctaa atcatccgtt tgaagaacga
    20221 gactagcata catgcgtccg ataaatcctg ttgctaccac cgcaaaaatc actgtaatag
    20281 caagtgaaat ccatgcttcc gctccctccg catagccatt aatcgttcga aacggcataa
    20341 agaaggtcga aataaaggga atataagaac caatcttcaa gaggagattg tcaccagctg
    20401 cacctagagc tgtcactcca aaaaaaccac ccataatcaa aatcatcaaa ggcgacaagg
    20461 ctttccctga gtcttcagga cgagaaacca tagatcctag gaaggctgcc aagactacgt
    20521 acatgaaaag actgatcaaa ataaagagca aggtattcag tgagatagca tctcccaagt
    20581 gatccaaaat accagactga gccaagaatg gcaaatcttt aaagagcaaa acggcagcca
    20641 gaccacctac aacatagatc ccaatatgcg ttaaaatcac tagaaacaga gccatcatcc
    20701 gcgcatagaa atagtgactt gcccttatgc tagaaaagac gacttccata attttggtgc
    20761 ctttttcact ggcaacttcc tgagctgtta cacccgcata ggtaatcaga atcatataaa
    20821 gaaagaatcc taaggcacct gctgcaattg tttgaataaa ctttttattt tccttggctt
    20881 catcaatctt ttctgtgaat tgaattgtct gcgctaagcg tttttcctgc tcttgagaca
    20941 aggaagcagt tgaacgatta agctgatttt gcagttcatt gagtgtacct gtaacctcaa
    21001 atttaattcc attttcaagc gatgtttcgc catgataaac tgcctttaga acactatctt
    21061 cttgatcaat ggtcaaataa ccttttaatt tttcttcttt aattgcttct ttggcacttg
    21121 cttcgtcttt atagtcgaag ttaacaccat ttacattctt cagtccttct gctacagatg
    21181 gcactgttgt cactactgcc actttattat ttttagccat agaagaacct tggagatgcc
    21241 caattcctac agagattcct aaaaagagga acggcgaaat caccataaag aagaaactcc
    21301 atgactcgac atgtcgaaga taggtttcct tgattacaac ccacatattt ctcatacttc
    21361 cactcctgat tctagtttaa agatttcatc gatagttggc gcttgttggt caaatgttgc
    21421 gatatattga ccttgagtca agattgggaa gagttccctt ccagcgctct catcctccaa
    21481 aatcaatttc caactgcctt gtttggtcaa gctcacctgt ttgacatgag gaagattttc
    21541 caattcttcc ttgcttcgtt cacttgaaac aaagagacgc gttttcccgt attgattgcg
    21601 gacatcctga actggtccgt gcaagaccac acggccatct cgaatcatca gaatatcgtc
    21661 acaaagttcc tcaacattgg tcatgacatg gtcagaaaag ataatggttg ctccgcgctc
    21721 tttttcctga aaaatgactt gtttgagcaa ttctgtatta actgggtcca atccactaaa
    21781 aggctcatcc aagataatca ggtctggttc atgaatcaga gtaataatga gctgaatctt
    21841 ctgctgattt ccttttgaca gactcttgat tttatctgtc agctttcctt ttacttccaa
    21901 cctcttcatc cattgaggga gtttttcttt gacttctttg gcatccatgc cttttagagt
    21961 cgccaagtag cgaacttgtt caagaactgt caatttaggc atgagactgc gttcttcagg
    22021 cagataacca atccgagcat agatctcctg acgaatatcc tgaccatcca gaccgatttc
    22081 tccctgatat tctaggaatt ttaaaatact atggaaaatc gttgtttttc cagcaccatt
    22141 ttttccgact agtcccaaaa tacgacctgg tcgcgcttga aagtcaatac caaacaaaac
    22201 ttgcttggat ccaaaacttt tctctagact tcttacttct agcatctttc acctccgaaa
    22261 tttcttgcac tcattatact cctttttgat agcctttaca atgttttttg tccattttta
    22321 gaagactatt gctgtgtaaa atatggcctg gagcactttt atactcaatg aaaatcaaag
    22381 agcaaactag gaagccagcc gtagactgct caaagtacag ctttgaggtt gcagataaaa
    22441 ctgacgaagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta
    22501 actatacgta cggcaaggcg aagctgacgt ggtttgaaga gattttcgaa gagtattagt
    22561 gataaatcca ttatacagca gcaaacttaa tttatacctt ccgctcctca actgtctatt
    22621 tttaatcctg aattgttatt tgagtaactc ctttttcctc gtaaagtttt cttcctctaa
    22681 aacttctgga aaaaggctaa tagtttcaga caacattttt ataagaaaca agttcatctg
    22741 tcatttcaag aaggagtaat cctttatcta ctaatggacg gaacagaatt caaccgcttg
    22801 tccgatatgt tttctaagga ttatatagta aaatgaaata agaacaggac aaattgatca
    22861 ggacagtcaa attgatttct aacaatgttt tagaagtaga tgtatactat tctagtttca
    22921 atctgctata tctattatgc acacccctat aggatctaat gaaaatcaca acaggctcat
    22981 tcatagatag ttacctcagg aaactaagaa aacgactaca aaggaagtcg cattcatcga
    23041 aaagtggatt aacaactatc ctaaaaaatg cttgaactac aagtccccca gagaagactt
    23101 ctggatgact aacttgaact tgaaatttag caataattaa ttcaccatct aactatattt
    23161 agtaattatt tcagaactga ttaatattaa aattaactaa caattcaaag gattcatact
    23221 agccataaat tacgtccatc agagagagac tcttactact tttagatttt agtctttcta
    23281 gcttcagaat acatctaaac tttagggaaa atgactattc gaaagcgcga atgcctcaaa
    23341 attatctcag ataagctatt cgaaacttag aatgctttta aatttatgga attgcgatta
    23401 ttcgaaacct agaatgcata taacctttag ttgacagacc tattctaagt ctcgaagggc
    23461 tatttacttt ctattcctta tcaaaaaaga ctcattcccc ctttctcctc caaaatatgg
    23521 tatagtagaa atatactatc tatgaggagt ttacatgtca caggataaac aaatgaaagc
    23581 tgtttctccc cttttgcagc gagttatcaa tatctcatcg attgtcggtg gggttgggag
    23641 tttgattttc tgtatttggg cttatcaggc tgggatttta caatccaagg aaaccctctc
    23701 tgcctttatc cagcaggcag gcatctgggg tccacctctc tttatctttt tacagatttt
    23761 acagactgtc gtccctatca ttccaggggc cttgacctcg gtggctgggg tctttatcta
    23821 cgggcacatc atcgggacta tctacaacta tatcggcatc gtgattggct gtgccattat
    23881 cttttatcta gtgcgcctat acggagctgc ctttgtccag tctgtcgtca gcaagcgcac
    23941 ctacgacaag tacatcggct ggctagataa gggcaatcgt tttgaccgct tctttatttt
    24001 tatgatgatt tggcccatta gcccagctga ctttctctgt atgctggctg ccctgaccaa
    24061 gatgagtttc aagcgctaca tgaccatcat cattctgacc aaacccttta ccctcgtggt
    24121 ttatacctac ggtctgacct atattattga ctttttctgg caaatgcttt gacacgtaaa
    24181 aaatccgttt ggtttcccaa gcggattttg tgctttattt tgaaacttct tttgcaagaa
    24241 caaagttccc aagtgtggca gaaccatttc ctgcgactgc tggcgtcacg atatagtcac
    24301 gcacatctgg tactggtagg taaccattaa gaagagatgt aaatttctca cggacacggt
    24361 ccagcatatg ttgttgagcc atgacccctc caccaaagac aatcacgtct gggcggaaag
    24421 tcactgtcgc attaaccgca gcttgagcga tatagtaggc ttgaacatcc caaacagggt
    24481 tgttgagttc aatagtttcc ccacgtacac ctgtacgagc ttccaaactt ggaccagctg
    24541 catagccttc cagacatccc ttatggaaag gacaaacacc cttaaactct ttttcaatat
    24601 ccattgggtg tctagcaaca taataatgac ccatttcagg gtgacccaca ccaccgataa
    24661 actcaccacg ttggatgacg cctgcaccga tacctgtacc gattgtgtag taaaccaagt
    24721 tttcgatacg accaccagca ttgttacggg caaccacttc accgtaagca gagctgttta
    24781 cgtctgttgt gaagtacatt ggcacgttta gggcgcgacg aagggcacca agcaagtcca
    24841 catttgccca gtttggtttt ggagtcgtcg tgataaagcc ataagttttt gagtttttgt
    24901 caatatcaat cggcccaaat gaaccaactg caagaccagc aaggttatcg aattttgaga
    24961 agaactcaat ggttttatcg attgtttcga ttggagttgt tgttggaaat tgtgtttttt
    25021 ctacaacgtt aaagttttca tcaccgacag cacagacaaa ctttgtaccg ccagcttcca
    25081 agcttccata taattttgtc atgataaacc tcttgttttt attttcttta ttatagcata
    25141 cttcgaaagt ctaaatgtct ctatttttta gattttcctc tgtaaatctt actatctaat
    25201 aaaaacgaac aaacatgtca tttgttcgtt ttcacattag agaggattga ttagattttc
    25261 acttcgatca cagcatcccc cttcgcaact gaacctgttg cgactggagc tactgaagcg
    25321 tagtcagctg tatttgtaac gataaccatt gttgtatcat caagtccagc tgtagcgatt
    25381 ttatttgagt caaatgttcc aagaacatcg ccaactttca ccttatcacc ttgagcaact
    25441 tttgcttcaa aaccgtcacc gttcatagat acagtgtcaa taccaacatg aatcaaaact
    25501 tcagcaccat ttcttgtttt caaaccaaaa gcgtgccctg ttggaaaggc aattgaaact
    25561 tcagcatcag ctggtgcata gaccacgcct tggcttggtt tcacagcgat accttgtccc
    25621 atagctccac ttgagaagac tgggtcattg acatcagcaa gagcgacaac atcaccgacg
    25681 ataggagtta caagtgtttc attttgaaga gctgctggcg caacttcttc tttttcttca
    25741 gccacttcag cttgttttgc agctgcagtt gcgtctactt catcttcgta accaaacatg
    25801 taagtaagag caaaaccaag ggcaaatgat acagctacca taagaaggta ttgtggaagt
    25861 tgtccgttac caacataaag cattgtacca gggatgatgg tgataccatt accagtacca
    25921 gcaagtccaa ggatagaagc caatccacca ccgattgcac cagcaatcaa tgaaaggaag
    25981 aatggtttac ggaagcgcaa gttcaccccg aagatagcag gctctgtaat acctaggaaa
    26041 gcagaaagag cagccgggaa agcaagtgtt ttcagttttg gattttttgt tttaacacca
    26101 accgcaacag tagcagcacc ttgagctgtc atagcagctg tgatgatagc gttgaatggg
    26161 ttagcatggt cagcagcaag taattgcact tcaagcaagt tgaagatgtg gtgcacacct
    26221 gacacgacga tcaattggtg aaccccacca atcaagaaac caccaagacc aaatggcatg
    26281 ctaagaatcg cttttgtagc aataaggatg tagttttcaa caacgtggaa aactggtcca
    26341 atgacaaaga gtccaaggat agacatgacc aaaagtgtca cgaatggtgt taccaagagg
    26401 tcaatgacat ctggaacaac cttgcggaca gctttttcaa atttagctcc gacaaccccg
    26461 atgatgaagg ctggaagaac ggaaccttgc aaaccaacaa cagggatgaa accaaagaag
    26521 ttcatcgctg ttacttcacc accttgagca actgcccaag cgtttggaag tgagccagag
    26581 acaagcatca taccaagaac gataccaacg gcaggatttc caccaaatac acggaaggtt
    26641 gaccacacaa ccaaacctgg caagatgatg aaggctgtat ctgtcaagat ttgtgtgtaa
    26701 gttgcaaagt cacctggaag tggcatttca agagcgttga aaagaccacg cacacccatg
    26761 aagagacctg tcgctacgat aactgggatg attggaacga aaacatcacc aaaagtacgg
    26821 atagcacgtt ggaaccagtt cccttgttta gcaacttctg ctttcatgtc atccttagat
    26881 gatgttggta atccaagtac aacaacttca tcgtacattt tgttaactgt acctgtacca
    26941 aagataattt ggtattgccc tgagttaaag aaagcacctt gaactttttc caagttctca
    27001 atcacttctt tattgatttt ctcttcatct ttgaccatga cacgtagacg tgtcgcacag
    27061 tgggcaacac tattgacatt ttcacgtccg cccaaggcat cgatgacttt ttttgcaatt
    27121 tcctgattgt tcatttgcaa aaatctcctt atataacatt ttgttcttgt ttgaaagcga
    27181 ttttattcgc ccttacgact attattttat catgttttaa aaatatgtca agcgttttgc
    27241 agaaaaaata ttctatctac ttagtgggca agctttagat tgattgtttt tgactgtttc
    27301 atcaggaatt tcttgaaaaa taagtttgaa accttggtta aaaagtcttt tatttcatta
    27361 tctttcacat tttcgtaaaa atttgattga tttcctgtat ttattttatg ataatcgttt
    27421 gacagttttt tctctttttt aacataaagg gcttgcattt tttatcgaaa acagttacta
    27481 ttaaaagtga taagattttt ggaggaatga atggaatgga caactgagcg tcgttacaga
    27541 ctttatcaag attggacgca agaagaaatt caacatataa aggaaaatat ggcacaatct
    27601 ccatggcata ctcattacca tgttgagcca aaaacaggac ttctcaacga cccaaatggc
    27661 ttttcttact ttgatggcaa gtggatcctc ttttaccaga attttccttt tggtgcagcc
    27721 cacggtttaa aatcttgggc acagctagaa agtgatgatt tggttcactt tagagaaact
    27781 ggagtcaaag ttttgccaga tactccatta gatagtcacg gtgcctactc tggttctgcc
    27841 atgcaatttg gcgataactt attcctattt tatacaggaa atgttcgcga tgaaaactgg
    27901 atccgtcacc cataccagat cggtgctttg atggacaagg agggtaagat tacaaagatt
    27961 gacaagatct tgattgacca gccagcagac tctactgacc acttccgcga tccacaaatt
    28021 tttaacttta aaggtcaata ctatgctgtc gtcggtggac aggacttgga gaaaaaaggc
    28081 ttcgtccgtc tctacaaggc tgtcaataac gactacacaa actggcaagc agttggcgac
    28141 cttgactttg ctaacgaccg tactgcctac atgatggaat gtcctaatct ggtctttgta
    28201 gaggaacagc ctgtccttct ctactgtcca caaggattgg ataagaaagt tctagactac
    28261 gataatatct ttccaaatat gtataagatt ggggcttcct ttgaccctaa aaatgccaaa
    28321 atggtagatg tgtctcaact tcaaaacatg gattacggtt tcgaagccta tgcaactcaa
    28381 gccttcaacg ctcctgatgg gcgtgctcta gcagttagct ggcttggttt gccagatgtt
    28441 tcttacccat ctgaccgttt tgaccaccaa ggaaccttct ctttggtcaa ggaactcact
    28501 atcaaagacg acaagctcta ccagtatcca gtcgctgcta ttaaggacct tcgtgcttct
    28561 gaagaagcct tctcaaaccg ttcccaaacc aagaacactt acgaacttga actcaacttg
    28621 gaagctaata gccagagcga gattgtctta cttgctgata aagaaggtaa gggactttca
    28681 atcaactttg accttgtaaa cggtcaagtg acagtggatc gtagccaggc tggagaacag
    28741 tatgcccaag aatttgggac aactcgttct tgccctatcg agaatcaggc tactactgct
    28801 acaatcttca tcgataactc tgtctttgaa attttcatca ataaaggaga aaaagtattt
    28861 tctggtcgtg tcttcccaca tgcggaccaa aatggtatcc tgatcaaatc tggaaaccca
    28921 actggaattt actatgaatt agattatggt cgcaaaacta actgatgtcg ccaaacttgc
    28981 aggcgtcagt cctactaccg tttctcgggt tatcaataaa aaagggtatc tatctgagaa
    29041 aaccatccaa aaagtcaatg aagccatgag agaattgggc tataaaccca acaacctggc
    29101 tcgtagtctg caaggaaaat cagctaagtt aatcggcttg attttcccca atatttccaa
    29161 tgttttctat gcagaattga ttgataaatt ggaacaccaa ctcttcaaaa atggttacaa
    29221 gaccatcatc tgcaacagtg aacatgattc tgagaaggaa cgcgaataca tcgaaatgtt
    29281 ggaagccaat caggtagacg gcatcatttc tggtagtcac aacctaggaa tcgaagacta
    29341 caatcgtgtg acagcgccga ttatttcctt tgaccgaaac ctatcgccag acatccctgt
    29401 tgtctcctct gacaactatg ctggtggggt tcttgctgcc caaaccttgg tcaagacagg
    29461 tgcccagtct atcatcatga ttacagggaa tgacaattcc aattcgccaa ccggactgcg
    29521 ccacgctggt tttgcatccg tactcccaaa agctcctatt atcaatgttt ccagtgactt
    29581 ttctcccgtc agaaaagaaa tggaaatcaa gaatatcttg acccgggaaa aaccagatgc
    29641 catttttgct tcggatgatt tgacagctat tctggtcatt aaaatcgctc aagaattggg
    29701 aatttctgtc ccaaaagagc tcaaggtcat cggctatgat gggacctact ttatcgaaaa
    29761 ttactaccct caattggcta ctatcaagca acctttggaa gagattgctt gtctcactat
    29821 tgatcttctc ttgcaaaaga ttgaaggcaa ggaagtcgcc acaactggtt acttcttacc
    29881 agttacgcta ttaccaggaa aaagtattta aacacaagaa aactcagacc gattcgtctg
    29941 agtttttatg atcttaaatt ttcgagatag cgctgggctg tctctaggtt aaaggtttta
    30001 tctgagatga ggcgctctac taggggagca acttcagatt cactagcccc agctaggaga
    30061 gctagagatt tggcctgtag tttcatgtgg ccttgctgga tgcccgtact taccaaggct
    30121 ttgagggctg caaaattttg agcaagaccg atggacacga taatctgggc taattctctg
    30181 gcagaaggat ttcctagtag atcatgactg agagctacac gtgggttgag gccgatagag
    30241 ccacccttag tcgctacagg catgggcagg gtcatctcac cgaccaattc ttctctttca
    30301 aggtccagcg tccagcagct aagaccttga tagcgtccat ctcgactggc aaaggcatgg
    30361 gccccagctt cgatggcacg ccagtcatta ccagtggcaa tcaaaatcgc atcaatacca
    30421 ttaaaaattc ctttattatg agtagcagct cggtaaggat cagcctgcgc aaactgacta
    30481 gccaacgcaa ttttctccgc aatctctcgt ccttgatcct tttggcggct caagtagcga
    30541 aaggccatgc gacagcttgc agtcaccaga gaatcagtcg cgtagttgga caggattccc
    30601 atgagactct gtccctgact gagttcttct aagactggtt tcaaggcttc cagcatggtg
    30661 ttgagcatat tggcacccat ggcttcctgg gtatcgacat gaatataaac aacgagaaag
    30721 tctggttcgc cttttatctg ctcgacatgc agatcacgcg ccccacctcc acgtttaacg
    30781 atagaaggat aggcttgatt ggcaagctcc aagagctccg ctttcttgct ggcaatcttc
    30841 tcttgcgcta gtttaggatt agcaacttga taaagggcta cctgcccaat catctgtcgc
    30901 tgatggactt gtgcagtaaa accacctgta cgcttgatga ttttgctggc atagctggcc
    30961 gccgcaacca cagagggttc ttctgtcaca tagggaacgg tgtattcctg atcgttgaca
    31021 agtacctccg gaaccagcga ataaggcaga gaaaaagttc ccaccacatt ctcactcagc
    31081 tggtctgcca cagtcacact catctgttca tccttctcca gactagcttg tctctcagga
    31141 ctaaggagcg cctgagcttt taacagctcg aggcgctctt ggtatgattt tttagaaaat
    31201 ccattccaac ttatcttcat tatttttcaa ccttgctata acggcgttgg tggtcgagaa
    31261 tttcaaccaa ggcaaaatct tgattttcat agccagcaaa ctgggcagag tttgtttcat
    31321 ccaagtttac ttcctcaaaa aagacctttt catagtctgc aacggatagg gcagttcgtt
    31381 ggttgagctt gttcaaacgg tctttatcca aataagcttc atatccttca accaattcac
    31441 cactgaagaa ctcagccaca gctccacttc cgtaactata aagggcgatt ttatccccag
    31501 ctttcaagct atctgtattt tccaagagag acaaaagtcc aaggaaaagt gaacctgtgt
    31561 agatgttccc caccttttga ctgtagagaa tagactggtc aaaatgcttt tgtaagaggt
    31621 cttttttctc ttgaggcagg ctcttatcca tgattttttt caagcctttt agcgctaatt
    31681 taggataagg caagtggaaa caaacagcag caaaatcatc caaagtaagc tggtagcgtt
    31741 tttgatattc aagccaagtc gttttcaaac tatccaagta ttgttgggta gaatagacac
    31801 catttacata aggagttgtc gagtaatttg gtcgccagaa atccatgatg tcacgggtct
    31861 gagctacatt gtcattatta aaggccatca tgcgtggatt ttgtgtaatc aacatagcta
    31921 cacttccagc accttgagtt ggttctcccg gagtttcaat accatatttg gcaatatcac
    31981 tggcaatgac caataccttg gactccggag aattttccac atgcaatttg gcataatgga
    32041 gggcagctgt cgccccatag caggcttctt taatctcgaa actacgagca aagggctgga
    32101 tgcccagcaa gccatgcaca aagacggccg cagccttact ctggtcaatt cctgactcgg
    32161 tcgccacaat gaccatgtca acttcttgtc tttcttgctc agttaaaata gagtcactag
    32221 cactggccgc caaggtcacg atatcctcag ttaggggcgc aatactcaat tccttgagta
    32281 agagtccttt acttaatttt tcagggtcaa ttcccctcgc ttctgctaag tcttgtaatt
    32341 tcaagacata ttgactggtc gcaaaaccaa tcttatcaat accgattgtc atatttacct
    32401 ctgttttatc attcatgtaa aaaatcgttc tatactattt tatcacaaat ggcagtaaaa
    32461 gagagaaaaa agacttgatt caccaaatca agcctcttat tggtcatcat tttaaagaat
    32521 gattagttgc tagagagttc accgatataa gtagctttat aagctccatt cacagttatc
    32581 agctcctgga ggatcaaatt tcctgagtaa gtccttccca tctcatctac aaatttttga
    32641 taaaactgac tggtcggaat ttctctgaca tccttatcaa atgtcttgtc aagtgtttta
    32701 ctaaccttct cagcaatcaa ttgatgctct taccatccac tttgaaactc tgagcccgaa
    32761 ctagaaacca tgactgggat aaacaacaag gtcagtagat ttacagacaa taagtaaagt
    32821 agtagacttc ctgcaaaact agaatcctag ttcatgattg ataataccag cactcaaatt
    32881 cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt gttgaaaaca ttttaaacgt
    32941 ttttactttg gcaaagatgt tctcaacctt gcttctctcc ttagatagcg catggttaca
    33001 ggctttatct tcagctgtta gcggcttgag tttgctggat ttacgtggag tttgtgcttg
    33061 aggatatatc ttcatgagcc cttgataacc actgtcagcc aagattttac cagcttgtcc
    33121 gatatttctg cgactcattt tgaacaactt catatcatga caatagttca cagtgatatc
    33181 caaagaaaca attctccctt gacttgtgac aatcgcttga gtcttcatag cgtgaaattt
    33241 ctttttacca gaatgattcg ctaattcttt ttttagggcg attgattttt actttcgtcg
    33301 catcaatcat taccgtgtcc tcagaactga gaggagttct tgaaatcgta acaccacttt
    33361 gaacaagagt tacttcaacc cattggctcc gacggagtaa gttgctttcg tggataccaa
    33421 aatcagccgc aatttcttca taagttcgat attctcgcac atatagaaag cgttgttaat
    33481 ttatttatct catttttcag aaaattcttt tatttctgta aagtctacga tactcgatgt
    33541 gtttttatat aatgatacag tctgagaatc actgttccgc tagccattcc aatagagatt
    33601 accagagcca acatgacaac caaggtcgca cttgccagtg ctttattata gtcccctgtc
    33661 acaaaaaagg cagttgttcg gtaggagaga taacctggaa ccagcggtgc caaaatggcc
    33721 aagataaaga ccacagcagg tgtcttataa agaatactta aaatctggct gacacaagaa
    33781 ccaataatgg ctgcaatgaa ggtagctaca atgacattgg tcggttcctt gagcaagaga
    33841 tagattagcc agacagtcat gcccaaaatc cctccaggca agagcataga ccgttgcaca
    33901 ttgagtacga ttaaaaaagt gataatggca agaaaacttg ctactgcttg taataaaaag
    33961 gttgttagtg tcatattagt tcatcaatac caaggcgaca gaagttcctg cccctaaagc
    34021 gagggtaatg agcagggatt caaacatctt actcatacca gagtttatgt ggttggtcat
    34081 aatatcacgg accgcattgg tcaaggcaat acctggtaca aacggcatga ccgcaccagc
    34141 tataatcaaa tctgccgttg aaggaaaacc tgtgtagcga gcccaaaact gggcaattat
    34201 cccaaagaca aaggctccag caaaggctgt cacaaaggga attcggataa atttttccac
    34261 atagagggaa aaggcaaaac caaataaggt cgccactcct gccccaagtg cgtcgtagat
    34321 atttccgcta aacataactg aaaagaaagg agcactaaag gtcgcagcca gagttacctg
    34381 caacttagta tagggaaggg gttgagcttg caaggccgtc aattgcttaa aggctgtttc
    34441 taagtcaatc tgccccccaa ctagctgacg agaaatctgg ttcacatcgc agactttttc
    34501 gatgttataa gaagaggagg tcacgcgctt catgcgcgaa atattggtat tttcaataga
    34561 gaaaaagata gcggcaggca tggcaaggac attgcaatcc acaatcccct gcgaatgcgc
    34621 gattcgaatc atggtatctt ctacacgatg gatttctgag ccacttttaa ggagaatagt
    34681 ccccgctagc ataatcacat caatgacggc atttaattct tttgattctt ccatgctttc
    34741 ctccttttat caactccctc tattctatca caaatccgga ctcaaaaaaa atctttgcca
    34801 tgaaatcatg acaaagattg attactcatt ttgattatcc atctgctttt aaggagtagc
    34861 tgaagttgtt ttaggtttgt agattgaaat cttgactcta gtcttattga ggtctacctt
    34921 ttcacctgct ctaggacttt gttcaacaac catgccttct gcactacctg caggcgctgt
    34981 cgtcacttct acaacttcta tattagcttc cttaatccca acaatttgaa tcaaattgtt
    35041 cttagtaaac tccaagctgg aaccaatgta actcggcatg gcaacacttg taactttttt
    35101 agctactgtc aagacaattt gagtaggttt actcacatca taagtcgttc cggcacctgg
    35161 actttgtttc ataatcgttc ctggttcgct ttcgctggac tcttcttcct ctatcttaat
    35221 caaattctca ggaaccttct tctgcttgag ttctgagatt acttctgtag agttccgtcc
    35281 aatatagttc cctaattgaa tcgtcgtagc ttttttagct actgtcaaaa caatttgagt
    35341 tgccttgctc aagtcatagg tcgtaccttc tggtagactt tgcttcagga ccgttccagc
    35401 ctcactctca ttcgactctt cttcctcaat tttaatcaaa ttatctggaa cttttttctc
    35461 ttttaattcc gcgataacat cagaagattt ccggccgaca taattactaa tttggaagga
    35521 ttgtttgcct gatgagacaa ccagattgat tttcgttcct tcttttcgac cagttccagc
    35581 gccaggatct gtacggataa tccgcccttc ttccaccttt tcactagcct ctgtcttctc
    35641 ctcaccaatc tcaaaattgg cttttttgag cgttgccttg gcctctgcaa ctgtctgacc
    35701 tgccacatct ggaatggcaa tggttgcagg agttctggat agtatccaaa taagagaagc
    35761 tgccaccaat acaaggctgg ccaacaaaat caggtaacgc atcttaaatc tatgtttttt
    35821 cggtgcttgt ggttggtaag tttcctctgt cacagcctgg cttgggtttt tgattgattt
    35881 gtgttctgtc tgcgcttgaa ccttaggaat agatgtcaag gtactctgag aaaccttcgg
    35941 caaggtcttg gtatctgcct tgctcgtttc atcaaagatt aacttacttt catttctacg
    36001 attgtaggac aagctactag acaagtccac atacatctct gaaaccgagc ggtagcgatt
    36061 ggtcaacttt ttagcagttg ccttgataat aacattttct aaagcctgag gtacagatgg
    36121 attttctgca ataacggacg gcaggggttt ctggaaatgc tggagggcaa tggtcaccgc
    36181 gctatccccg tcataaggga tatggcctgt caacatctca tagaaaataa tccccatggc
    36241 atagatatca ctctgcacag tcgccttaga accacgcgcc tgctctggtg acaagtaatg
    36301 aactgagccc aacatcgagt tagtctgggt cagacttgtc tctgcaaagg ctacagcaat
    36361 cccaaagtct gtgaccttgg ccgtcccatc tggtgtcaaa aggatatttt gaggtttcaa
    36421 gtccctgtga acaattcctc gagtatgggc caagcgcata gccaagagaa tttgtcccat
    36481 gatacggact gcttcttcat tagaaagagg ataatgttcc ttgatatagc gtttgaggtc
    36541 tagtccagca acatactcca ttgcaagata ctgttgaccg tcttcctcac caatatctgt
    36601 tatccgaacg atatgaggat ggtctagatc tgccatagct ctcgcttcac gctgaaaacg
    36661 agctacagct atcgggtccg tctggtagtt ggtcctcaga accttcactg ccacttcttc
    36721 cccatctaag attaagtctt tggctaggta gacatccgcc atacctcctc gaccaatctg
    36781 tttgacaatc cgatagcgtc cggcaaaaat cttgccgatt tggatcattc tgcatcctcc
    36841 tcgttcatag aaacaagggc aaccgtaatg ttgtctaaac ctcctgcatt gttagcaaaa
    36901 cgaacaagtg tctccgtttt atctgctaaa ggaatatcac tggttacaat atcacgaatc
    36961 tcactgcctg aaatcatgtt ggtcaagccg tcactattga gcaagagata gtcacctgac
    37021 tcaaggataa ctgtcccaaa atcaggctga atttcatctt tttgcccaat agactgggtg
    37081 ataatatttt tttgcggatg agcttctgcc tcttctggtg tcaattgacc agccttgagc
    37141 aattcattaa ccaaagaatg atcactcgtc aactgatggt attcttctcc acgaatcaag
    37201 ccgatacgcg aatcaccaat atgagcatag atagcctgat tatcaataat agcaaggact
    37261 tccaaagtag ttcccatgcc tctgtaagct tcatcctgac caagctggtg aatcttttga
    37321 ttttcaattt ctaggtaatg ggcgaaccat tcacgcactt cattgactgt atcgatctga
    37381 gtatcaaccc aagctacacc caggtctgtg accgccattt cactagcgat attccctgcg
    37441 cgatgacctc ccatcccatc agctaaaata atcatggtac gtccagctct attgacatag
    37501 tggttgacat agtcttggtt atttgttcgt ttctgaccaa catctgttaa taatgaaatt
    37561 tccatgtgtc agttccttcc taatccgata tcttgcgaaa ttgactgatg aagaatccat
    37621 cacttccata caattcaggt gtaatgagga tacagccgtc tttcatgata tccttacatt
    37681 catgttctag ttttacctgc tcgaactcgg gatgactctc taaaaaggcc ttaacgactt
    37741 gaaaattctc ctctgagacg atagtgcagg tgctataagt tattatacca cctttgccta
    37801 gtatttgaca aacactacct aatatttcta actgaatttc ctgcaaggac gcgaaatctg
    37861 ccgtttcttt attgtatttg atatctggtt ttcggcgcaa aagaccgatt cctgaacaag
    37921 gagcatccac caaaatctta tcaaaggaat cctggtcaaa aaactcatgc acctttctgg
    37981 catccaattt ttgagtttga acccgatctt caactcccag acgttgggca ttttcttgaa
    38041 ttaaatccaa cttgtggtcg tacaagtcca gagcagtaac ctgacctgtc gtaagataag
    38101 aggctatatg ggctgttttc ccacctggag ccgcacaggc atcaagcact cgctcatcac
    38161 cttgtaaatc aagcgtcgga gcaaccagct gactggactc gtcttggatg gtaatggctc
    38221 catccgcaaa caaattatgc cctgcaaaat gcccttgctc cttaaccaga ccagtggttg
    38281 ctaaaaggga attattcgcc tccaacaagg cttggatttc ctcttttcga cttaggtctg
    38341 ttacacgaat actggctttg tttcgcacta acaggctttc aaagatggct tttgctctct
    38401 cctctccgta ttcttccttg agtttggcaa ctagccaaac tgggagagaa taggcaatgg
    38461 agtcacgctt gttttttcgc ttgatgctag caatatctgg ccagccttca cgcaagatac
    38521 ggcgaaggac agcgttgacc aatttttcac tgcctttttt acggagtttg gccaattcca
    38581 ctgcttcatt gaccacagca tgatctggaa tcttgtccaa atagcggagt tggtaggcac
    38641 tcatgagaag aaggacatag agccagctgt ctaactggtc tctgtcttcg ataaagtggg
    38701 ataggtacca ttccagagtc agtttacggg ctaccgttcc atagactagc tcggtcacta
    38761 agcccttgtc tgctgcagag agctgactcc ccttgaggtg tttatttaag gcaatatttg
    38821 aatatgcttg attcacaaaa acatcctcta gcactgctag agctaaactt ctagccgttt
    38881 ctactttagt caccaaatcg ttctcctaca gtcaatgtac gtccaacccc gttgaggaag
    38941 gaagcaatgt ccatcttagg cttaccagct ggctgcactt gtttgaggga tagagcccct
    39001 tcagccgttg cgacaatcaa ttctttcttg cagatagaga gaatctcacc tggatttccc
    39061 tgaccttcta ctggtagggc ttcataaatc ttaaagcggt cgcccttaag aaaagtatgg
    39121 gcaacaggcc aggggttcat tccacgaatt tggttaaaga gttgacgatt ggttttgttc
    39181 cagtccagtt tttcttcctc tggctttata tttggagaga aggtaacctg actcgtatcc
    39241 tgcggttcag gtttgatatc accagcaata taggcaggca gagtgtccaa aagcaaatca
    39301 cgaccaacta gcgccaattt ttcaaacaag gtgccaacat tgtcctcatc tgtgatcgga
    39361 atgctgcgac gagaaatcat atctcctgca tccatttcct taaccatttc catgatggtc
    39421 acaccagctt cctcatcccc ttgaatcaag gcataatgga taggcgcacc accacggtgt
    39481 ctaggaagga gggaggcatg aacgttgaca gcaaagtcca tgctatcaag gagtttgctt
    39541 gggagaaact gcccaaaagc agcagtcaca attccatctg ctcctagctt cataagttct
    39601 tccatctctg gacttccaga taatttttca ggttggtaga tagatagtcc tgcttccttg
    39661 gcagcctgct tgactggggt ttcttggata acttttttac gaccaacagc acggtctggc
    39721 tgggtcacaa cggctagaat ttcgtaacgg tcatctgtca aaagtccttt taagactgtt
    39781 gctgaaaagt cgggggtccc cataaagatt agttttgtca tatcttctcc ttcttataaa
    39841 aattgctgcg gctcatggtc aatgctgaga cggagctcac tattttcccg ttcttgagtc
    39901 aaggctaaaa cctggttgag ggtcggcccc agctcatctt ctaaacggta tttaattaaa
    39961 atctggtaat gatagaggtt gtgggtacgg gcaatcggtt ttggcgttgg ccccagaatg
    40021 ggactggtct ctgacaagcc tgaccgcaaa atgttcatga cttcataggc acgtttgaaa
    40081 acctcttctt ctttcttgtg agaaagggta ataccaatcg tgaaatagta aggtggatag
    40141 ccgagttgtc gtctgattcc catttcatag gcataaaagc cttcgtaatc ttgatccttg
    40201 gcaaatcgaa tagcatagtg ctgcgaattg taggactgta tcaagacttg acctgctttt
    40261 tcagcccgac ctgctcgacc tgccacctga gtcaagagct ggaaggttct ctcagaagaa
    40321 cggaaatcag gcagattcaa ggccgtatcc gcatttagaa ctccgactag ggtaacattg
    40381 ggaaaatcca aaccctttgc aatcatctga gtaccaagta aaatatccgc ttcccctcgc
    40441 ccaaactggt caagcaaggc ttggtgactg cctttctttc gagtcgtatc cacatccatc
    40501 ctcaaaatgc gagcttgggg aaagagttct gctagctcat cataagcctt ctgagttccc
    40561 gtcccatagt aacgaatact gcggctctta cagttaggac agacctgagg aatatccttc
    40621 gagaaaccac aataatggca gttcatagtc ttggtatcca tatgcaaggt cagagaaata
    40681 tcgcagttgg gacaagtatc caccgtccca cactcccgac acatgacaaa gctagaataa
    40741 ccacggcgat tgagcatgag aaccacctgc tcttttttaa ccagacggtc ttggatagcc
    40801 tctagcaaag gaggcgtaaa gtttgacgtc tcattttgtc cgatatagtc tcgaaagtca
    40861 atcacttgaa cctcagggat tgtagccaaa ggattggcac gttgggttag acgtaagtgt
    40921 tgatagacgc ctttgccagc acgtgcccgg ctctctaagc tcggcgttgc agatccaagt
    40981 accagagttg cttgattata ctgagcccgt aaaatagcta cctctctggc atggtaacgg
    41041 ggattgctgt cctgcttata agccgcttca tgctcttcat caataatcat gacacccaga
    41101 tttttcagag gagcaaagat agcagatctg gcaccaacaa caacttgggc atcgccacgc
    41161 tccaccttgc gccattcatc atacttttca ccattggata atcctgagtg aagaatggct
    41221 accttgtccc caaaacgtgc tataaaacgc tcggtcatct gaggagtcaa ggaaatctca
    41281 ggtaccagca aaatagctgt cttgccctta tccagggcac cttggataat ctgcaagtaa
    41341 acctcggtct tcccacttcc tgtaatccct tgaagtagaa agggaggttg agaactgcca
    41401 atagaactca caaccgcatc acgcgcctgt ctttgttctg gatttaactc caaaggtcta
    41461 cttgcttcaa ttccttcaaa ataagcagcc gagcgtcgaa cttccttttg gactatggta
    41521 acagcacctt gatccacaaa gaagttgact tgctctcgcg agtaggactc taacaagcta
    41581 gccaaggaag cgctctctgg atgagacagc agataatctc tcagttccaa ctttttcttg
    41641 gcacgtgtag aaatctcaac accttctaat tgggcatggt caacctcata ccaagactgg
    41701 gtcttgacct tcttttgatc gactgcctga tattccagac caagcaggcc ttttctagtc
    41761 aaacgcatca tttcagcttg cttacctaaa tccagagaag aaaaggctag cgaatcttct
    41821 gaaccaaaca ggcgcactcg ttcttcctga ctcaagcctt ccagaggata gagaatcttg
    41881 tcatagctag aattcagaaa tcctggaagc atggccttga ggatagagat tttgtaggag
    41941 aagacagatt tgcgtaactc ctcagccagc cagagttgtt ctggcgtgag aacaggagaa
    42001 aaatccagca cctctgcaat atcttttaaa tcttgctcca tctcttctcc atctgattgg
    42061 gactccaaac caagaacaat cccttgaatc aggcgattac ccttaccaaa aggtacatga
    42121 acccgcatcc caacttccag cattccctca aattcctccg gaatcctgta actatagggc
    42181 tggtccgtct gcatcaaggg cacatctacg ataatcttag ctagggccat cttctcacct
    42241 cctccttgtc agtacattct tgcaatagaa aaaataagat tgagtccccc caaccttaaa
    42301 ttttttcacc atcttctttt tctttagcaa tttgctcttt gattttcttt tcttcttctt
    42361 ctttgcggcg tttttcttct tcgatacggc gacgcactgc ttcacgtttt ccttctggat
    42421 ctgggtgaat tgtaacgttt cctgattcga tttcttctaa agcgcgaaga gttgattttt
    42481 cagacttgaa accttgagtt gctggggcac ctgcttccaa ttcgtgggca cgttttgctt
    42541 ccaagattac gagtgaatat tttgaaggaa ccttgtcgag caaggtatca atagagggtt
    42601 ttaacatcat ttgcttgtac ctattttcta aattttatcg ggtagttgga gattttggta
    42661 acatctcctg atagtgacca atgacacgat ccacacagaa atgttctgct tcaatcacac
    42721 atttgacacg ttcagcaact aggggcacct gatcgttgac aatggcataa tcatactcac
    42781 gcatgagggc aatttcttcc ttggcctttt cgattcgttg ggcaatcact tctgcactat
    42841 ctgttccacg acctaccaag cgatcttgca attcatccaa atctggtggt gtcaggaaga
    42901 taaagacagc atctggaacc tttttcttga cctgaagagc accctgaact tcaatttcaa
    42961 ggaaaacatc gattcccttg tccaaggttt cattgacata ggtcagagga gttccatagt
    43021 agttaccgac atattctgcg tattccaaca tctgtccttg acgaatcagc tcttcaaatt
    43081 cttcacgagt acggaagaaa tagtcaacac cgtccacttc tccaggacgt tgtgcgcgtg
    43141 tcgtcatcga tacagagtat tgaaattggt tttcagaact ctcaaaaatc tctcttctaa
    43201 ccgttccttt tccaacccct gaaggaccag aaaaaacgat tagtaagcct cggtctgcca
    43261 ttgtgtctcc ttttagtcaa tctgtgaaat aacatttctc tagaataatg gcaaaaagcc
    43321 agattatcct ttacagtctt tctatctagt gtaacaaaaa agcagtaatt tttcaactgc
    43381 tctttcttat ttatttagca taatctactg cacgaagctc gcgaatcacg gttaccttga
    43441 tatttcctgg ataatcgaga ttgttttcaa ttttcttacg aactttgtga gccaagattg
    43501 tgactttgtc gtccttgatt tttcctggat tgaccatgat acgaatttca cgtcctgctt
    43561 gaagggcaaa gctagtttgc actccttcaa agccgttagc aatttcttcc aaatcatgga
    43621 gacgcttgat gtagctttca agagactcac tacgagcacc tggacgggct gcgctcaagg
    43681 catctgctgc agcgacgata actgctatca cgctctcagc ttcaacatct ccgtggtgac
    43741 tagcaatcgt attcaccaca actgggggtt ccttgtactt acgggccaat tccataccga
    43801 tttcaacgtg gctaccttca acctcatggt caatggcttt cccgatatcg tgaaggaatc
    43861 cagcacgacg ggcaagagcc gcattttcac caagttcgct cgccatgata ccagccaact
    43921 tagcaacctc aatcgaatgg cgcaaaacat tttgtccata tgaagtacgg aactgcaaac
    43981 gtcccataat cttcatcaag tctggatgaa ggtttggcgc accaatttca taggcagcag
    44041 cctcaccgta ttcacgaatc ttattgtcaa tctcttgacg gtttttctca accaactctt
    44101 cgatacgagc tggatgtata cgaccatctt tgagcaacat ttccatagtc atacgggcaa
    44161 tctcacgacg aatcggatca aatcctgaca aggtcaccac ttctggtgta tcgtcgataa
    44221 tcacatcgac ccctgtcaaa ctttcaaagg tacgaatgtt acgaccttca cgaccaataa
    44281 tgcgtccctt catagtatcg tctggcagat gaactgttga gtttgttgac tccgctacat
    44341 attcaccagc gatacgttgc atagcttgaa ccaagatgtc cttagccatt ttgtcagaac
    44401 gttccttgac ctcttgctca gcttcgcgaa tgcgactggc aatctccctg gtcaagtttt
    44461 cctctgtctg agccaagata atatctcgtg cttctgcctg agacagagca ccaatacgct
    44521 ctagttctgc ttctttttgt ctttcgactt cctctaattg ctcttcacgc gcatcaaggt
    44581 ttttcgctct atcagaaata ctttgttctt tttgttcaag tgtttgttct ttactcgtca
    44641 aattgtcgtc cttacggtca aggctagtag ctctctctgt caaacgactt tcgatttgtt
    44701 tgagttcttg acgttctgat ttgaattcag cgtccacttc ttcacggtat tttctggctt
    44761 cttctttggc ctccaatagt gcttcttttt taagagactt gctttcacgt ttagcttcat
    44821 taacaagtaa atccgcttca cgctcagctt gtccacgtaa attagttgct tcttgttcag
    44881 catttaaaag catcaactcc gcagcttcct gagatgattt catcttagct gagatgctga
    44941 catatccaat gactaaacca atgatgacgg caaaaacagc aatcgcaagc gacatgattt
    45001 ccatgttttt acctcatttt attgttattc cgaatgacat acattctttt acattctacc
    45061 ataaaaaagt gattttcaca aacctaaaat agaatatgtt ttggggaatt tagtctgaat
    45121 tttaggatta cacttccttg gttttttaag gagataagtc agtgtcgcta tcaaaaagtt
    45181 ccaccgaatt tctacaacta tggattttct attgttgtgc caaatagtgt tccactagat
    45241 tcaaacattt tgctttcaag tctttgaaaa ttcacttta
//
LOCUS       DAVKWS010000009        44521 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKWS010000009 DAVKWS010000000
VERSION     DAVKWS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.009"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(364..1092)
                     /locus_tag="VW852_000561"
     CDS             complement(364..1092)
                     /locus_tag="VW852_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0271746.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1250..2659
                     /locus_tag="VW852_000562"
     CDS             1250..2659
                     /locus_tag="VW852_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0271747.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2677..3972
                     /locus_tag="VW852_000563"
     CDS             2677..3972
                     /locus_tag="VW852_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0271748.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3977..4285
                     /locus_tag="VW852_000564"
     CDS             3977..4285
                     /locus_tag="VW852_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0271749.1"
                     /translation="MKQILLVCNAGMSTNMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4282..4590
                     /locus_tag="VW852_000565"
     CDS             4282..4590
                     /locus_tag="VW852_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0271750.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(5379..8030)
                     /gene="adhE"
                     /locus_tag="VW852_000566"
                     /gene_synonym="adhC"
     CDS             complement(5379..8030)
                     /gene="adhE"
                     /locus_tag="VW852_000566"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV0271751.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(8330..8740)
                     /locus_tag="VW852_000567"
     CDS             complement(8330..8740)
                     /locus_tag="VW852_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271752.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(8742..9170)
                     /locus_tag="VW852_000568"
     CDS             complement(8742..9170)
                     /locus_tag="VW852_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV0271753.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(9216..9515)
                     /gene="yajC"
                     /locus_tag="VW852_000569"
     CDS             complement(9216..9515)
                     /gene="yajC"
                     /locus_tag="VW852_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0271754.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(9632..11608)
                     /gene="tkt"
                     /locus_tag="VW852_000570"
     CDS             complement(9632..11608)
                     /gene="tkt"
                     /locus_tag="VW852_000570"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0271755.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMNEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEVHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            complement(11721..12812)
                     /gene="ulaG"
                     /locus_tag="VW852_000571"
     CDS             complement(11721..12812)
                     /gene="ulaG"
                     /locus_tag="VW852_000571"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV0271756.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(12924..14597)
                     /locus_tag="VW852_000572"
     CDS             complement(12924..14597)
                     /locus_tag="VW852_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0271757.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            complement(14774..15478)
                     /locus_tag="VW852_000573"
     CDS             complement(14774..15478)
                     /locus_tag="VW852_000573"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV0271758.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(15480..16343)
                     /locus_tag="VW852_000574"
     CDS             complement(15480..16343)
                     /locus_tag="VW852_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV0271759.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            complement(16347..17012)
                     /locus_tag="VW852_000575"
     CDS             complement(16347..17012)
                     /locus_tag="VW852_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV0271760.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            complement(17029..17514)
                     /locus_tag="VW852_000576"
     CDS             complement(17029..17514)
                     /locus_tag="VW852_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0271761.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(17591..17872)
                     /locus_tag="VW852_000577"
     CDS             complement(17591..17872)
                     /locus_tag="VW852_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0271762.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            complement(17895..19352)
                     /locus_tag="VW852_000578"
     CDS             complement(17895..19352)
                     /locus_tag="VW852_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0271763.1"
                     /translation="MEVVSNVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            complement(19481..20104)
                     /locus_tag="VW852_000579"
     CDS             complement(19481..20104)
                     /locus_tag="VW852_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV0271764.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMIALRLYGNPSDF
                     GISLEVSFIERKKDEQTLGKQAKVLEIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYQVTRG"
     gene            complement(20155..21141)
                     /locus_tag="VW852_000580"
     CDS             complement(20155..21141)
                     /locus_tag="VW852_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV0271765.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(21160..21984)
                     /locus_tag="VW852_000581"
     CDS             complement(21160..21984)
                     /locus_tag="VW852_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0271766.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(21959..22330)
                     /gene="rnpA"
                     /locus_tag="VW852_000582"
     CDS             complement(21959..22330)
                     /gene="rnpA"
                     /locus_tag="VW852_000582"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV0271767.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            complement(22479..23669)
                     /locus_tag="VW852_000583"
     CDS             complement(22479..23669)
                     /locus_tag="VW852_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV0271768.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(23720..24673)
                     /locus_tag="VW852_000584"
     CDS             complement(23720..24673)
                     /locus_tag="VW852_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0271769.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYTHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSNLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(24734..25328)
                     /locus_tag="VW852_000585"
                     /pseudo
     CDS             complement(24734..25328)
                     /locus_tag="VW852_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(25465..25878)
                     /locus_tag="VW852_000586"
     CDS             complement(25465..25878)
                     /locus_tag="VW852_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0271770.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQVFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            complement(25856..26317)
                     /locus_tag="VW852_000587"
     CDS             complement(25856..26317)
                     /locus_tag="VW852_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0271771.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            complement(26280..26540)
                     /locus_tag="VW852_000588"
     CDS             complement(26280..26540)
                     /locus_tag="VW852_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271772.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(26545..26949)
                     /locus_tag="VW852_000589"
     CDS             complement(26545..26949)
                     /locus_tag="VW852_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV0271773.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTNLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(26942..27268)
                     /gene="comGC"
                     /locus_tag="VW852_000590"
     CDS             complement(26942..27268)
                     /gene="comGC"
                     /locus_tag="VW852_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV0271774.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVND"
     gene            complement(27270..28286)
                     /locus_tag="VW852_000591"
     CDS             complement(27270..28286)
                     /locus_tag="VW852_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV0271775.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LVMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(28234..29175)
                     /locus_tag="VW852_000592"
     CDS             complement(28234..29175)
                     /locus_tag="VW852_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV0271776.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(29251..29616)
                     /locus_tag="VW852_000593"
     CDS             complement(29251..29616)
                     /locus_tag="VW852_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV0271777.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(29767..30825)
                     /locus_tag="VW852_000594"
     CDS             complement(29767..30825)
                     /locus_tag="VW852_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV0271778.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(30988..32139)
                     /gene="nagA"
                     /locus_tag="VW852_000595"
     CDS             complement(30988..32139)
                     /gene="nagA"
                     /locus_tag="VW852_000595"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV0271779.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(32292..34109)
                     /locus_tag="VW852_000596"
     CDS             complement(32292..34109)
                     /locus_tag="VW852_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV0271780.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(34210..35352)
                     /gene="tgt"
                     /locus_tag="VW852_000597"
     CDS             complement(34210..35352)
                     /gene="tgt"
                     /locus_tag="VW852_000597"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV0271781.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            35482..36339
                     /locus_tag="VW852_000598"
     CDS             35482..36339
                     /locus_tag="VW852_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV0271782.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIHLLFYSSFLAKKFVTLHPEFP
                     SLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(36367..37011)
                     /gene="pcp"
                     /locus_tag="VW852_000599"
     CDS             complement(36367..37011)
                     /gene="pcp"
                     /locus_tag="VW852_000599"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV0271783.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLETIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPI
                     RLDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRQTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(37118..37477)
                     /locus_tag="VW852_000600"
     CDS             complement(37118..37477)
                     /locus_tag="VW852_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV0271784.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(37488..37826)
                     /locus_tag="VW852_000601"
     CDS             complement(37488..37826)
                     /locus_tag="VW852_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV0271785.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            complement(38340..39452)
                     /locus_tag="VW852_000602"
     CDS             complement(38340..39452)
                     /locus_tag="VW852_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0271786.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(39618..40238)
                     /locus_tag="VW852_000603"
     CDS             complement(39618..40238)
                     /locus_tag="VW852_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0271787.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(40242..41522)
                     /locus_tag="VW852_000604"
     CDS             complement(40242..41522)
                     /locus_tag="VW852_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0271788.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(41583..43067)
                     /locus_tag="VW852_000605"
     CDS             complement(41583..43067)
                     /locus_tag="VW852_000605"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV0271789.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDTSYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(43143..44420)
                     /locus_tag="VW852_000606"
     CDS             complement(43143..44420)
                     /locus_tag="VW852_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV0271790.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
BASE COUNT        13277 a         9680 c         7930 g        13634 t
ORIGIN      
        1 aaattgggag attgaaattc atttctagca atttgttaga agtcacagtc tactattcta
       61 acctcagtat actatatgtg ttacaagaaa agactcaaaa acttctcgtc agtgtaagaa
      121 tgtttgaaac actatttaaa actaaataac tataagaagt agatcaggtg aacaatttca
      181 gtacctactc tataagctat acgaagatat ttttcaacat gatggataaa acgatcactc
      241 ttttaaagcc atcttattta gaaaagagaa accatttaag catagatggg accttcttgc
      301 cctgttttaa atttttctta aactagagtc tgtaaaagat tggcttcatt ttcacatttt
      361 ctcctaggag gaatgtcgta atgcaaaaga attaaattca aataaatcat agcgatgacg
      421 actaatcgag tactcaaacg gagttccgtt atccaaataa gccacttgtt ctacttcaaa
      481 taccggttcc gttggaagga gctgcaagta atgttgctca ttttctgaac tagaagaagc
      541 tcgcaagatt tttgtagcac tggcaatatg caatcctaac tttccttcaa tgtaattgta
      601 aatcgatttt tgtaaaatat cttcagtaat acctggaata acatcggtac tcatataagt
      661 ttgttccaga acataaggtt taccgtcaat aatacgcagg cggtaaatat tataaactgg
      721 actctgcaaa gcgacctgta gtttttctgc taaaaattca tttgcaaatt ctaatttgaa
      781 gtgaataatc ctcgagtcaa ttgtagaatg agcatcttca gatatttttg tcagtccccg
      841 gatatctctt tctggaacga ttaattttct ttttgagctg ccacgagaga gaacaaaggt
      901 tccctgccca cgttttctaa aaattaagcc ctcagaaact aacaagtcta aagctttttt
      961 gatcgtcatt cgactacagg caaactgttt agctaatgtc atttcatcag gcaattgttc
     1021 gttcatggta tatttattac aaagaatctt ttcacgaatc tcattgtaaa taaataaata
     1081 ttttgtactc atttaatctg gttctctcta taatattcta atagtagtat aacaaagttc
     1141 aagaaatgag gcaaacacaa gatatgtata aacaattttt aaaaatgtct agacaaattt
     1201 taaaattatg ctatacttta tgtatagaat aatagaaagg aggttgagca tgaaaaaaaa
     1261 gttagtattt cctaatctgt tttggtgggg ggctgcttct agcggacctc agacagaagg
     1321 tcaatatgga aaagtacatg aaaatgtgat ggactactgg ttcaaaacgc atccagaaga
     1381 ttttttcgat aatgtcggac ctcttgtagc cagtaacttt tttcatactt acaccgaaga
     1441 tttccacttg atgaaggaaa ttggagttaa ttctttccgc acttccatcc aatggagtcg
     1501 actcatcaag aatttagaga caggtgagcc tgatccaaaa ggtattgctt tctacaatgc
     1561 catcattgaa gaagctaaaa agaaccagat ggatcttgtg atgaatttac atcattttga
     1621 tttaccagtg gaacttcttc aaaaatacgg tggttgggaa agcaaacatg tagtggagtt
     1681 attcgtgaag tttgccaaga ctgctttcac atgctttgga gataaggttc attactggac
     1741 aactttcaat gagccaatgg tcattccaga agcaggatac ttatatgctt tccattatcc
     1801 aaatctaaaa ggaaagggaa aagaggccgt acaaatcatc tataatctaa accttgctag
     1861 tgcaaaagtg attcaactat atcgctcatt aggacttgat ggaaagattg ggattatttt
     1921 aaacttgaca cctgcttatc caagaagtaa ttctccagaa gacttagaag caagtcgatt
     1981 tacagatgac ttctttaaca aagtcttctt gaatccagct gttaaaggaa ctttcccaga
     2041 aagattggta aaacagctag agagagatgg cgtgttatgg agtcataccg aaaaagagct
     2101 tcaactgatg aaatcaaata cggttgattt tcttggagta aactactacc atccaaaacg
     2161 tgttcaagca caagcaaatc ctgaggaata tcagacgccc tggatgccag accaatactt
     2221 caaagagtat gaatggccgg agcgtcgcat gaatccatat cgtggttggg aaatttttcc
     2281 gaaagccatt tatgatattg ctatgattgt gaaggaagaa tatgataata ttccatggtt
     2341 tatcagtgaa aacggaatgg gtgttgaaaa cgaagcacgg tttatcggtg aaaatggagt
     2401 tatcgatgac gtgtatcgta ttgaatttta tgaagaacat ttaagatggc tacataaagc
     2461 cattgaagag ggaagtcact gttttggata ccacgcttgg accgcatttg attgctggtc
     2521 ttggaataat gcatataaga atcgttacgg atttatctcc gttgatttag aaacgcaaaa
     2581 gagaaccatc aagagctcag gaagatggta tcgcaaagta agtgacaata acggttttga
     2641 agtagaaatt gaggagtaaa gagaggagat attcagatga aaaactcaaa atttatagat
     2701 caatttgcca cctttgctgg taaactaggg aaccaaattc atttaaaaac cctaagagat
     2761 gcattcgtaa cagtaatgcc attatatatt ttggcaggtt taatcgttct tttgaacaac
     2821 acggtattta agtggatttt ccaaggggat acattaacaa gattccaata ttggggaata
     2881 acaattgcaa acggtacttt aagtatttca ggtatgatta ttgctgtaat ggttggttat
     2941 ttcttagcta aaaacagaga tttcgaaaac ccgttagcag catcaatgct atcattagtt
     3001 tctttaattg tgatgatgcc aaatacagtt tctgtagttc ctgacggagc aaaagatgcg
     3061 gtaaacattt caggtgttct ttcattcaac aacacaggta caggcgcaat gttcgccggg
     3121 gttatcgtag cgattattgc aacagaatta ttcattgaat tatcaaacgt taaagcttta
     3181 caaatgaacc ttggtgaaaa tattccacca gctgttagta gatcatttag cgtattactt
     3241 ccagtcatga ccgtcatctc cttatttggg gttgtttcag cattattatt caatataact
     3301 ggaatgaact taatctcaat cattacaatc tttattcaag aaccaattcg tcatattggt
     3361 acaagcttaa tcggggtcat tattatttac tctttaggaa atatgttatg gctatttggt
     3421 attcaccaag cagttattta cagtgccatc ctagaaccat tactattaat taacattact
     3481 gaaaacatca ctgcagcaaa taatggacaa gccattccac acatcatcaa cctatcacaa
     3541 atacaaacat tcgctttaat gggtggtagt ggatctacat tatgtttatt aatagcaaca
     3601 ttcttagtga gtcgcaatgc tgtctctaaa aacgtggcta aattatcttt tggacctggt
     3661 atcttcaata tcaatgaacc agtattattc ggttacccaa tcgtttataa catttcatta
     3721 gctattccat ttatcacagt tccagtcctt ggtattttaa tcagctactt agcaacagtt
     3781 acaggattca tgagtcctgc atttatacaa gttccttgga ctacaccagt attcttaaat
     3841 gcatggttag caacagcagg ggacgtgaga gcagttctag ttcaattcat catctttgca
     3901 cttggagttc ttctatacat tccatttatc aaagttaatg acaaagttgt tgagcaagaa
     3961 atggaaggtt aagattatga aacaaatttt attagtatgt aatgcgggaa tgtcgacaaa
     4021 tatgcttgtt aaaaaaatgc aacaaagtgc gactgaacgt gggattgaaa tttctatcca
     4081 agcaaaatca atgacagaag ctaaaaagaa tatccatgaa gcagatgtca ttctaattgg
     4141 gccacaaatt cgttatgagt tacttgctgt gaaagaaatt gcaggaaata tccctgtaga
     4201 taccattgat atgcgggatt acggaatgat gaatggagca aaagttctgg aacaagcact
     4261 tgcatggata ggagaaataa gatgacagaa gaaatggaat atatctgttt tcagctcatt
     4321 gccaatagtg gagcagccaa atcatccttt attgaagcca tccaactagc taaggcagga
     4381 aatttaaaag aagcgaaaat taaagttgaa gaagcggaag actccttagt agaagcgcac
     4441 aagattcact ctaatctcat acaaaaagaa gctactggag aaaaaatagg attctccttg
     4501 ctatttatgc atgcagaaga ccaaatggct tcaacagaga ttattcagtt gctatctaaa
     4561 gaattcatcg atctctatca aaataaatag gaacactgtc gttcccatct gtctttatta
     4621 atgacggcta cattgtagga acctccaaaa gttggacaaa atgattgaaa ggattggatc
     4681 ctgcattaca caagatccaa tccttttctt ttactctagt ttttataatg tgttgaattt
     4741 agaatagtcc actgcagttt ctaaaaaatt gctggaaata gatgttaagc tccaatttat
     4801 ttgtttatat cttatttcag tttactatac tagcttatta ttctagtgaa aaattgaatg
     4861 agccactatt tatttaatat gtatgatgag ggaatgactc aaaaacttct tgtcgatata
     4921 agaatatctg agatagtatt ttaaaacgaa aggattataa aagctcattt ccttcgcact
     4981 tattttatag agatctatca ttatactctt tagaaatcaa aaagcaacac accttgtcat
     5041 cgttttctcg tttgctcttt gattttaatt gggccttagt aaatactact cttaaattaa
     5101 actatagtga attgaaataa gatgtgaaca actctatcag aaaagtaaaa ttaatttcta
     5161 taaatgtttt agcagcagat gtgtactatt ctagtttcaa cctactatat attttgtttc
     5221 tattctagtt tttcaaaaag ggcaaaaagg cctcaacatc ataaaaaggc atcctatcag
     5281 gtattcaaaa accttgatag gatgcctttt atcatagaaa aattactgga tctttccctc
     5341 aaattgagtt tttgtcttgc ttctagactg ataaacaatt atttacggcg tcctggtctt
     5401 tctttgtagc catagtatgc atcttcgatg atttcttgca tatggtctac cattggaaga
     5461 cgtgggttag ctggtgaaca ttggtcttca taagccagga aggctaattt acgagaatgt
     5521 tctttccatt ctttttcgtc aattccttgg tctctaaaat tcatttggat cccaatacgt
     5581 tcaccgagtt cgtagacagc ttttgcgtaa gattcaaccc cttcttctgg agtagaagct
     5641 ggaagtccaa gcatgcgtgc gatatcttgg tatttttcat ctgcacggta gtagttgtac
     5701 ttaggccatg ttgctgtctt agctggacgt gtaccgttgt aacggataac gtacggaagc
     5761 aagatggcat ttgtacgtcc gtgaactgtg tggaattgcg caccaatctt atgggccatt
     5821 gagtgagaaa tacctaggaa ggcattggca aaggccatac cagcgattgt tgaagcgtta
     5881 tgcattttct cacgtgagtg gaagtctgca ttcttaactg agctttcgag attttcaaag
     5941 accaatttaa tagcttgaag tgctaaacca tcagtgtagt cactagccat ttgtgatacg
     6001 tatgcttctg tcgcgtgagt caatacgtcc ataccagtat cagcagcaac aaatcctgga
     6061 actgtcaata ccaaagcagg gtctacaatt gccacagttg gtgtcaatga gtagtcagcg
     6121 attgggtatt tacggttgtt tgctttatca gagataacgg caaatggtgt tacttcagat
     6181 cctgtaccag atgtagttgg aatcgcgatg aatttagtct tcttaccaag caatgggaac
     6241 ttgaaggcac gtttacggat atccatgaat ttttggacaa ggtcacggaa gtccacttct
     6301 ggttgctcgt agaaaagcca cattactttg gcagcatcca ttggagaccc accaccgagt
     6361 gcgatgatgg tatctggttt gaaggcacgc ataatctcag taccacggtt tacagttgtg
     6421 atatctggat ccggttctac atccgcaaag atttggtaaa caaccttatt gcgacgaagg
     6481 tccagttgtt cgatgatacg atcaaggaaa ccaagctcta ccatggcatg gtcagtaacg
     6541 atcatgacac gttcaacgtc acgacatttt tgaaggtatt gaattgaatc acgttcaaag
     6601 tatgtttttg aaggaagttt catccattgc atgttatttc tccgtcttcc gacttttttg
     6661 atattcaaga ggttaatggc actaacgtta tccccaactg agtttcgtcc gtaagaacca
     6721 catccaagtg tcaatgatgg caagaaggca ttgtaaacgt ccccgatacc accaaaagta
     6781 gaaggtgagt tacagataac acgaatagct ttaacagctt taccaaattc tttagtcaat
     6841 tcttcgtcag ctgtgtggat agctgctgag tgtccaagac cgttaaattc aaccatttga
     6901 cgagccttag taataccatc ttcacggctt tcagatttca aaactgcaat aactggtgac
     6961 aatttttcac gagtcaatgg ctcattttcg ccaacttctt tacattctgc agcaagaatg
     7021 tttgttcctt ctggaactgt aaatcctgct tgttctgcaa tccaagttgc tggtttacca
     7081 acgatgtcag cgttcaattt tgcaccagca cagtttttgc tgtttgcttt gacgccgaag
     7141 cagaactctt caagaagagc tttttctttt ttgtttacaa agtaagtgtg gtaagatttg
     7201 aactctgcta caaattcatc gtaaatttct ttatcaatga taactgcttg ttcagatgca
     7261 cagaccatac cgttatcaaa tgatttagac atgacgatat cgtgtgctgc ttgacgaatg
     7321 tttgctgatt tttcaacata agctggaacg tttccggcac ctaccccaag agctggttta
     7381 ccacatgaat aagccgcctt aaccatggca ttaccacctg ttgcaaggat tgtcgcaaca
     7441 ccttcgtggt tcataagggc acttgttgct tccatagatg gttgagtaat ccattgcaca
     7501 cagttttcag gagcaccagc tgcgatagct gcatcgcgga cgatacgagc tgcatgagca
     7561 gatgattctt gtgctgatgg atggaaggca aagacgattg ggttacgtgt cttcaatgaa
     7621 atcaatgatt tgaagattgc tgttgatgtt gggtttgttg ttggagtaat accacaaaca
     7681 acaccaactg gttcagcaat aagagtcaat cctgttacat cgtcttcttc gataacgcca
     7741 actgtcttag tgtgacgcat gttgtttact acgtgttcac aggcaaacaa gttcttagtt
     7801 gctttatctt caaatacacc acgtcctgtt tcttcaaagg catgtaaagc caattctccg
     7861 tgggcatcca aagctgctac tgatgctttg gcaacgatgt agtcaacttg ctcttgatcc
     7921 aatttacgca tttcttcaag ggcaactaga gctttttgaa ccaactcatc tacgtgtttt
     7981 tcagcaacga gtttcttttc ctctggtgtc acagtttttt tatcagccat attttcctcc
     8041 acagctttca aggatcgata tcctttgtta attttttcac aagtttatta taacgtatta
     8101 tttcaacttt gtaaacagtt tcataaacat ttcacaaaga atttttaaac tcttgtgaaa
     8161 cttttctcat tttcttttct tatcaggttt tatctttgaa gtttgtgaaa tttatttcaa
     8221 atatttttta tttcacaaac ttgcttggaa gagggatttg gaaagaaaga gatttacagg
     8281 taagcgctta ccttgtgatt ctaataatac ctcctttgat aaggaggctt tatttttgtt
     8341 gaaaaacgcc ttgtttaaaa gttccttcat aaacgacttc ttgttctgtt gttagtttcc
     8401 cttttccttc agcctgacca tttacaaaat caccttcgta ggtccagcct tctttagatt
     8461 gaaaggtacc ttttccgttg aaggctccat tgttgaagcc acctgtatat tggtctccat
     8521 tttggaaggt aatggtacct tggccattca ttttaccacg gacaagactg ccatcataaa
     8581 caatcgttcc attatcgagt tttaagacac cttttcctgg aatatttaga aaaaagacga
     8641 gtacagcaca gaaaacaatg gtaactactg ccaaaagctc taaacgtgga cgagtcagat
     8701 agacacgata cttttcgtaa aaattcgtaa gattttccac cttaactctc cttttctaaa
     8761 cgttctaacc aagcttgaca gccctcttga acacgtcgat aggtttcttc aaaatctcct
     8821 gtataccaag ggtctggaac actttcagat gaaaatgagt aaatcttatc ttgacagtct
     8881 actggacaca tctgacgtaa gtcagaaata tttgaagcgt ccattccgat aatataatca
     8941 aaggcttcaa aatcttcctt actaatctga agcgatgtct tgttcttgtc ataaggaatc
     9001 tcatactctt gaaaaattcc ctgagtcccc ttatgaatcg gattgccatg ttcccaagag
     9061 gaagttgctc gactttggat ttcgtagtta tctgtcattg atttcataac aaactcggcc
     9121 atagggctac ggcaaatatt tcccagacag acaaagacta attttttcat cccatttcct
     9181 ttcttttata gaaaacggga gttcgtgatc ccgtcttatt tttcaattgc gccttctagt
     9241 gaagctgttt ctttcagcgg taatactgtc ttgatagcag ctagttcaaa agtcaagtaa
     9301 actccatcta cgtcaagaac tattgttccc ttctccgtat ctacttcatc gactgttcca
     9361 taaagtccac cgattgtaat cacttcatag cctttttgta gtttatttaa gctttccata
     9421 cgcttttgag cttgtttctt ttgagaacgt tgcataaaga acatcaaggc catcatacct
     9481 acaagcatga ttaaaaaagt aatatttgga ttcattgttt tctcctttgt cttttacata
     9541 ggactactat atcaaatttc agctactttt acaagattgt accttacttt tctggtaaga
     9601 aaaaccagag cttacgccct gatttttagg attatttcaa atttcgaaca acttttacaa
     9661 gattttcgac agtaaagcca tattctgcca atacttttgg tgctggggca gaggctccga
     9721 aggtatcaat acctagaacg gcaccatcga gaccaacata tttgtaccag ttttgacttg
     9781 cacccatttc gactgcaaca cgacggcgga ctgcatttgg aagaatttct tccttgtaag
     9841 ctgcatcttg tttatcaaag acatctgtag atggcatgct gactacgcgg attttttcgc
     9901 cttgactagc caattcttta gcagctgaga cagcaagatt aacctctgaa cctgtcgcaa
     9961 tcaagatggt atcaaagtcg gctgcatttt catatacaac ataagcacct ttagcaacct
    10021 tgtcgaagtc tgttccatct tcaacagtca agttttgacg tgtcaagaca agggcagttg
    10081 gtgttttctc acttgtcact gcaaggtacc aagctgcttg cgtttcacgc gcatctgctg
    10141 gacggaaaac atttagattt ggcatagcac gaagacctgc taaatgctca actggttcgt
    10201 gagtcggacc atcttcccca actgcgattg aatcatgggt aaagacataa gtcacaggaa
    10261 gtccttgtaa ggctgacaag cggacagctg ccttcacata gtcagagaag acgaagaaag
    10321 ttccaccgta tacacgaagt ccaccatgaa gggccatccc gttcaagatt gttcccattg
    10381 caaattcacg aacaccaaac tgaatgttac gattcaagcg attggcatcg tcttgaagtc
    10441 catccgtctt gatataagtc atgtttgagt gagcaaggtc agctgatcca cctaggaagg
    10501 ttggtaattt agctgccaca acattcaagg catcttgact tgaattacga gttgcttgag
    10561 aaaaaccatt ttctaaagct gggaagtctg ctggagtcac ttcgactgga tcacgtccgt
    10621 cgatgatggc ttctacttct gcagccagtt ctggatgaac ttctttataa tctgcaacta
    10681 atttagtcca agcttgataa gctgatgcgc cacggtctgc aacatgttct ttgaaatcag
    10741 catatacttg ttctggaatt tcaaatggtt cgtagtccca accgagggct tggcgagttg
    10801 atgcagtttc atctgctcca agaggggcgc cgtgtacagc attagttcct tgtttgtttg
    10861 gagaaccgta tccaataacc gtcttcactt caatcaaaga tggcttgcct gaagcttttg
    10921 ctgtttcgat agcagcatgg atggcttcca agtctgttcc attttcaacc aaggcagtat
    10981 gccaaccgta ggcattgtaa cggtcacgaa cactttctgt aaaggaatcc tttgtctcac
    11041 catccaagtt gatatcattt gaatcataaa gaacaaccaa cttatcaagt ttttgcaaac
    11101 ctgcgtatga agctgcctcg cttgagacac cttccatcaa gtctccgtct ccacagataa
    11161 cgtaagtata gtggtcaaag atattgtagc cttcacggtt atatttggct gccaagaaac
    11221 gttctgcttg ggcaaaacca gtagcagttg aaatcccttg ccctagagga cctgtcgtag
    11281 catcaatccc tgccgtatga ccaaattctg ggtgacctgg tgtttttgaa ccccattgac
    11341 ggaagctctt aatctcattc atgctgacat cttcaaaacc agaaaggtga agaagagcat
    11401 aaaggagcat tgaaccatga cctgctgaaa gaataaagcg gtcgcggtta atccaatttg
    11461 gttgagctgg attgatatga agttgttttg taaagaggct gtaagccatc ggagccgctc
    11521 ccataaccac acctggatga cctgagttgg ctttattaat ggcgtcaata cctagaaaac
    11581 gaattgcatt aacagataga tttgacatag aatttccttt ctattgaggt gattattgaa
    11641 tcacacattc tattttacta ttatatgaaa attatccgta tcatgcaaca tacggataac
    11701 ctccaaagaa tatttttata ttatagcaaa gctttaaatt gaatgttaga gtcttgttca
    11761 aaacaatcat caaaaccacg tggatgatgg tattctacta agtgttgatc ttgaggataa
    11821 gtgtacttac cgccaacttc ccagataaat ggatggaaat cgtattgcaa gcgatctttt
    11881 cgcattttcc aaagttctag aatctcatta gtagaagcca tgaagttaga ccagatatca
    11941 tagtgaactg ggataatgac tttggtacgc agattttctg ccatacgaag aaggtcgata
    12001 gatgtcattt tgtcttggat acctaccgga ttttcaccat agttattcaa agcaacatca
    12061 attttaaagt ctttaccatg ttttgcaaaa tagtttgaga agtgagaatc tgcaccatga
    12121 tagatggttc cacctggtgt ttcaaagata tagttaacag ccttttgagc catttcttca
    12181 tctgtaacag ccaagccagc aagttcaccg cctgtctcat cagcaccgtt cactgggaga
    12241 gttaccaagc aagtacggtc aaatgattct actgcatgaa tcttcatatc ttttaattcg
    12301 atagtgtcac ctggtttaac aacgatgata cgttcttttg gaacacccca tccttcccag
    12361 attcgtccac agtggtaagg accgataaac ttaacatgct ctaacttagg attgttgaga
    12421 attgctgcag ctgtgtatgg gtcgatatga tcactgtgga agtgtgaaac taagtaatag
    12481 tctagttcgt tgatagcaaa tggatcgata accattggct gaacacgcaa gtttggttgc
    12541 aacttacgaa cacctgccat atttgccatt tggtgcccac gaaccatatc tttcactttt
    12601 ttggttgatt ttccacggtt tgaccaaagg tccatgacaa cgttagcacc agctggtgtc
    12661 ttaatccaag taccacagtt gcctagccac cacatggcaa agttgccttc aggtacgact
    12721 tcttcttcga tttcttcgtt caaccatgtt ccccactctg ggaaagtggc taaaatccat
    12781 gactctcttg taatttcttt gacgtttggc atatgttgcc tcctttggat ataattgtta
    12841 tctttgagat tagtatacca cccttcaaaa cactaaaaaa gaccaagaaa aacacaggtt
    12901 tgtgttcatc attggactgt ccttcaaata tcttccaaaa aagcttgaac tactatttct
    12961 ttatttataa gtgtttgaac ctcttcttgg aacttactag ccaacttgct gtctacaata
    13021 taagacgaaa taagactaga aagattttca ctgaaacttt tgctcttatt acgaaatata
    13081 ttgtgtttaa gatagtctag cattttcaaa atatcttctg cttcaaggat aggattaacc
    13141 cttaaaatcg gaaatctgct atccaaatca tcattagtag taatcactac atcaacttgt
    13201 gcaatatctt ccacactctt aaattgttct gttgtaaata cagtgtcaat ttgctcattt
    13261 ggaaaataaa gtttgcattg tttcagcaaa agtctcgaaa ccgcaacacc ttcatcacaa
    13321 acgagataaa cttttttcat atttttttga gacgatggtg tatactttaa aaatcctcca
    13381 atatgaatcg tcaaataggc aatatcatcg tctgttagcc gaataaacca tgctccttct
    13441 aaaatttccg cagattttct agtgactaaa aataattctc catacttgga tcgaatttgt
    13501 tttgttagag gatttttaga aaaaataccg taagtctttc taaataacaa ggctttacag
    13561 tggatcatca aatttcgtaa caaatcatcc ttgttctcaa tctccattcg gatttgtgat
    13621 tcaaaatacc agataaattc ttctaaagca agttttaatt gccgaaaatc atcactttct
    13681 gcatgaatat ccaaatcttt acgataggag aggaggagaa cagctacaag agaaacttca
    13741 agtcctgaca aagaaatttc aaacttttga aacaaccgtt ctcctaattt cttagacacc
    13801 tgatactcta ttctttttct tatcaaagaa aattctttct ctatatcctg atgtctttct
    13861 tgatactgtt caacattatg acagcttaac agcaaataag gtagaacctg caacataaaa
    13921 gttatttcat gatggtttat tttcttccct aaatcttgtt caactaaagg aacctgttcc
    13981 ttaaaaaatt ggttcatttc aacagaaagc aaacactcat catctaacct ctctttcatc
    14041 ttatcttcta aaatagatac aaaagtggca ttttcttcca taaaaatatg atatagaagc
    14101 gattgaagat actgaatttt attaagaggg tgggcatgaa ggtggtatcc ctgtgacttg
    14161 ctcacttgca aggtcacctg atattgttcc aaagttagtt gataacgaat actattcaaa
    14221 tcattgagaa cagtattcct agatacctct gttaactcta tcaatttttc aatagtaata
    14281 cgttctttag aaatacctat ccatagtaac attatcatca tacgttcatg cgcactcatg
    14341 atataatcgt acgaatctac ttccgataat agtttacaac aagcatctct ctgctcttcc
    14401 gttaagtgaa taccaattcg tggaatacta atgatgtgaa gcgcctcgtc aaccagagca
    14461 gcattgattt tgtcaatgtg ataataaatt ttccttcttg actctttcaa atctttcgaa
    14521 atcgccataa tcgttttgga cgtttcttga tccatcaaat attgaagcaa atcacaactt
    14581 gccttatcca gtaccatatt aaaaaatccc ccaaatctat tgatatcaca gcaattcttt
    14641 ttattttacc atatatatca agcgctattt ctaaaaaatc tttcaaaatt acttcaactc
    14701 caactttact ctcaataaaa atcaaatgta aaaagaggag ctaaatttat ctttttctcc
    14761 tccttcatcg ttcttacttt tgaccataat aagcatttgg tccatgttta cgttggtagt
    14821 gtttttctag tatgtactgg ggagcaggtt caactcttgg attgatttgt tctgtaaagc
    14881 gattcatctt tgatacttcc tctagtacga cagagtgata aacagcattc tctggatttt
    14941 tgccccaggt gaatggaccg tgattgcgta caacaattcc tggtacttca accgggttaa
    15001 gtccgcgatg ttcaaactct tctacgataa ccaggccagt atctttttca taggccactt
    15061 ctacttcgtc cttggtcaaa ctacgggcgc aagggattga accgtagaaa taatctgcat
    15121 gggttgttcc gtagaaagga atatcacgac ctgcctgagc ccaagcaaca gcttctgtcg
    15181 aatgggtgtg aaccacacta ccaatttctg accaagcctt atataattgc acatgagttg
    15241 ggaggtcgga agatggtctt aaatcccctt ctaggatctt accatctaga tcagtcacta
    15301 ccatgttttc aggtgtcaat tcgtcataat ccacgcctga tggtttgata acaatgacac
    15361 cgagttcgcg attgacttca gatacattcc cccaggtaaa tttgacaagt ccatgttttg
    15421 gcaatgattg attggcatca cagactcgtt tacgcatagc attgattact tgattcatct
    15481 tacatcaaac ctgctttctt aatgagtgga tagagaaaag cttgcgcctc ttgaatggct
    15541 gcgcgtgttt cttctactgt ttcacaattt tcagaccaca tttcgattag gaaaggtcca
    15601 ttataattgg tttcctttaa aatatcgaaa gcttcttccc atttgacaca accttgccca
    15661 aaaggtacat ctcggaactg gccctttgaa ctttctgtca ctgcataagt atccttgaga
    15721 tggagagctg cgatggcatg atgaccaaga taaaactcac tatagatatc attatgccat
    15781 gcagacacat taccaatatc tggatataca aagaggaagg gagagtcaat ctctttttct
    15841 atagccaaat atttttcgat gctattgatg aaaggatcat ccataatttc aatagcaagt
    15901 accacctgag cttcttcagc ccagtcacag gcttttctca aatttttgat aaaacgttgg
    15961 cgtgtctggg gtgacttttc ctcatagtaa acatcgtaac cagctaattg aatcgtacga
    16021 actcccaagt cttgagctaa ttcgatacat tttttcatga gttctagaga tttttcctct
    16081 agaactgaat cttttgatcc caatgggtag cgacgatggc ctgaaaaaca gatagaagga
    16141 atacgaacac cagtttcata gatttctttg acaacttcca agcgttcttc cttactccag
    16201 tcaagtcttg ctaaacgctc gtcacgttcg tcaatagaca tctcgataaa atcaaagcct
    16261 aactccttgg caaaatttaa acgttctagc caagtaaagt gtgttggggt tgccttttca
    16321 taaattccaa ttggacgtac catgatttac ccccaaattc gtttgatttc atccttgaag
    16381 gcacgcgctg ctcctgctgg atccacagcc tctgtaattc cacgacctgc gataaaggta
    16441 aagacatcaa caccttcaaa gagtttgaga gtatctacat ctagaccacc tgttacagat
    16501 acacggaagc ccatgtcaat gagtttttta accttattaa ggtctttttc accccaagtt
    16561 tcaccagcaa gaagagcatc acgagattgg tgataaatag cttgtgagat acctgcatct
    16621 agccaaagct gagcttgttc aaaagtccaa tcgccataaa gctcgatctg gatttcgcct
    16681 cgttctcctc gttcagtctt gatagccttt agagctgctt ccatagtagg gatggttgca
    16741 caacagatac aagtcatcca gtctgctcca cgaaccgcat tatttttagc aactgttcca
    16801 ccagcatcag cacattttgt gtctgccaca ataatcttat ctgggaaaag gctacgcaag
    16861 acttcagcca gttcacttcc aacttgaagc aagcaaacag ttccagcttc gataatatct
    16921 acttcctgac caacagaaac agctgcttta atcgctcctt gcaagtctga atggtctaat
    16981 gcaacttgta aattaggtat tctttttgtc atttctttat tcctctttct aactttccaa
    17041 atccaatcct tcgagataag ggctatcctt agattcttca atcatagcca agacatcttc
    17101 tttagtctgg caagcctgta aacgtgcaat agaatcttct aattcaaaca gggcaataat
    17161 ttgtggaatg gctacacttg tgtgaatttt tgaacttgtt gctgctagtg ccaacaaaac
    17221 agatacctct ttcccatctg aaaatacaac aggattttgt aaggtaatta atgaaaaggc
    17281 atcactttgc acaccagctt caggtctagc gtggggcata gccatacctg gcatcaagat
    17341 atagtaaggc ccatactctt cagtcgattc aatgatagca tcgtaatact ctggcaaaat
    17401 tgccccactt tcaatcaagg gatctactgc taccttgact gcttctttcc aatcgttagc
    17461 ctctaaacct agtcggatcg agtcattgtc aattaaagct tgttttaaat tcatatctgc
    17521 ctcctttatt taaagaggga gaaggacaga aacgataact ctcagtttct gtccagcccc
    17581 ctccaacctt ttactgtagt gcttgactga gtttttcggt gatttcttta tcatccatca
    17641 agttatcaag cccaattaac ttcccattag ttcgcccttc caattcttga atcaaatgaa
    17701 gagaagcgat tacgatgtca tatcctactg ctaaaccttt agcttcaccg acactgcatg
    17761 aattgactgt aaaatctgtt tgattaagct tacggagagc attttcaacc ttcatcttga
    17821 taaccattga tgaacccatt ccatttccgc acgctgctaa tactttaacc attttatttc
    17881 tccttttcta gatactaagc ttcttcttga acttcaccgt tgtaatattt ctctttatct
    17941 tttgctttgg caaattgaag ttgaggaata acaagcaaga agagacacac aagtacataa
    18001 ccaacaatac caaggtattt gaagatatat ccaaatccaa gccatgggaa ttcaaagtcg
    18061 atatttccat ggtagccacc ataagatgcc aaatcgagaa gggccacaca aagagctcct
    18121 agagcaactt gaaggacacc tgatataaag gaaaggataa cagccgcttt ccatccgccg
    18181 cgtttatcag cgtagaccgc aatggctgca ttgtcaaaga acactggtac aaatcctgta
    18241 ataataagaa tcggattttt aaagacgatg agcaagacaa ttgtaatcaa ttgaccaatc
    18301 aaaccaaagg taaatcctga caagacagca tttggagaac caaatccata agaagctgca
    18361 acgtcaaccg ctgggaatga acctggcaac aatttgtttg aaataccttg gaaggcgttt
    18421 gtcaactcag atacgaacat tcggacacct tgcatcaaaa cgaacaagta aactgagaag
    18481 gtaaaggctg tttggataat gtacataaag aaatcttgtt tagcaggatt gaatagagtt
    18541 cctgaagtga tgacttcttt attagacata atgtctggac ccaagattaa aagaatggct
    18601 ccgaagaata cgagcatcaa ggtagcagat gcaacaactg tatcgtggaa gattgagagg
    18661 aacttaggta atttaagatt gtctaaactt tcttctttct taccaaagcg tcctgctact
    18721 ttatctacaa accagattgc aaattgctgt tggtgaccaa tcgcaaatcc gccaccacca
    18781 gtcaagcgtt gagttgcctc aacagtcata tttgaactaa ctgcccagta aagtccacag
    18841 atgataccaa tcgctgctgt accgtaagca ttgcgcaatt gtggtactaa gaatagaacc
    18901 ataagagata ctgttgcagc ttgttgtacc atgatgtgac cagtaataaa gagggttctt
    18961 accttcgtaa tctttcgaag agctacgagc aagatattta ttccaaaacc aatcaataga
    19021 gctgtagttg cagttccaac aaaatctgga aactctgctg caattttgtt gtttgctgca
    19081 gcaagtccaa agtaagggtc gataaccgct gcaccaattt ggaatttgta gttaagagct
    19141 gctaagattg gacgaaaggt tgtaaccaaa ccaccagcac ccacgttaag caacatatac
    19201 cctactgttg ctttaacaaa ccctgaaaaa acgtcatggg caggtttttt caaaagtgca
    19261 tatcctatca acaccaataa acctacgaaa aatgcgggat tctgcaaaat attgctagaa
    19321 aaccaattta gaacatttga aacgacttcc ataattgttc tccttttgaa ttgtttttgt
    19381 aagtttatta tataatgcga aagcgcaaac aaaaagacca agaattacac agcggactgt
    19441 tcactcttgg actgtaatct tataataatt gactgacacg ttatcctcta gtcacctgat
    19501 aatagggaag aattttagaa taagacttca ataagccttc tacgatttct tcttcagacg
    19561 aattttctgt cataggaaca tctaccttca ctaaaacttt tcgaacttct tgacttctca
    19621 ccttctcgcg taaagtacga cgcttttctt cattcgcctc ccaccgttga ctttgaccat
    19681 tagagtaggt tagataataa atccctttaa cggttggaat ttctaaaact ttggcctgct
    19741 tgcccagtgt ttgctcatcc ttcttacgtt cgatgaaact gacttctaag gaaataccaa
    19801 aatcagaagg atttccatac aaacgcaagg ctatcatagg ttctgtcact tgtccgtctc
    19861 tctgtagata aacccaaaaa tgtggtctca aacgctgggc ctgattcatc cactgactag
    19921 tctgttggag ttgccattct ggatggcttg cttgaaaggc tttggccagt tctgtaaaag
    19981 cctttcgagc ctcttgtcct ttgtggcgga atgccagcat cttctctcgc tctgctcccg
    20041 ctttatcagg attacggtac tgcaaaccag caaagtctaa atagtctctt atcttattca
    20101 acatcagttt aacctcctct agctagcaaa aaatcaggtc aaccctgatt ttacttattc
    20161 tgtatctaca acgacatagc gatttggctc atcaccttca gagtaactgg tcacgccatc
    20221 catacgtgaa ataatacggt ggataatctt gcgttcacta ttcgacattg gatctgtttg
    20281 atgactacgt ccttcttcta aaacacgaat cgccaatttt tgcgcatagg tctgcaagac
    20341 ttctgcacgg tgttcaacat aatcattgac attaatagtg atatagaagg ttcttgaata
    20401 gcagttgtaa agataatttt gagccaacag ttgcaaggcc ttcaagactt taccatggta
    20461 gccgataata cgacctggtt cgttagtatc aatttgcaaa ttgatgctac gacggttata
    20521 atcatttgaa agtgtagcct caacatccat gtcatcaata atcgtttgaa cataagccat
    20581 tacttccgta gctacttgtt caatatcaaa gttcgtttca actttcaagc ccaagtcttc
    20641 tagttcttga ctttcagctt ggtcttgctc agtttcaagg tcatcagcgc ctgcttcttc
    20701 cctcatcgct tcctcgattt gaagttcatt taaaatctca atgtgaccag tttcttctaa
    20761 gatagtgctg gcatgtcttt catgttttaa gatttcagcc ttgacttcat cagaaatacc
    20821 ttgaccttct tcctctattt ttttaatagc atcaaccaca tgaccaaggt caacggtttc
    20881 ttcactaacc gtcttcacag gctcgttcaa atcattgatt tttttcggaa cgccttttac
    20941 tacctgttga tttgctttga caacagtcgt ttcactaatc gcttcaatat ccacttgggc
    21001 tggtttttta ccaaatagac caagaaagcc ttttttctcc ctagaaatga ctttgatatg
    21061 agccttcatt cttggaatat ctaattcttt caatcctttc tggattgctt cttcaacagt
    21121 tgaacctgta aatactacca ttaccagatt cctccttatt tatttcgttt tctgagcctt
    21181 tttcttagct tttctttttc tattttccaa atctttttgt gcctgtacta cggcctcgcg
    21241 ctctgcgata atcttgaatg gattattcaa gaaataggtt tgcaagactt gataagcatt
    21301 agacactgtc cagtataggg cgactccacc tggcgcataa actgcaaaga taaaaatcaa
    21361 gactggaatc ccatacatca tcgcagtcgt agcgccattt cgctcagaca aagctttgtt
    21421 ggacaaccaa gtacttaaaa aggtgaatac tgctgctaaa atcggaagaa caagggttgt
    21481 atccacacta ccaaggttaa tccataagaa atgacctgtc tttaaaaagt caactcttga
    21541 tagggcttgg aacagggcca aaataaccgg catctgaatc aaaatcggcc aaagagagtc
    21601 tgactgtctg acacccattt ctttaaatac tttacgcatt tcctgctcta gtttggttct
    21661 gctttccata tctcgacctg gatattgttc tcgaagcgcc ttaatgcgtg gctgagcttc
    21721 ctgcattttc ctagaagcca ccatttgcac ctgaaagact ggcaagagga ctgtacgaat
    21781 caagaccgta aagagaataa tccccactcc gatactaata tcaaacgata aaaagcgaat
    21841 aatttccgca aagaagtaaa ccaatttact ccaaaaatca gccgattcgg ctgtaatatc
    21901 gctagttacc ccattagtcg cacaggctgt catgattaac agagacagtc ctagcaaact
    21961 agtcaacttt agtttctttt tcactcccat ttccttcccg gtaaatcttt gataatttta
    22021 atacatggag tagatttttc tccatctctg cgtatcccaa ggtttcgact ccttttcgag
    22081 caatgacaac aaagtcgaca tcttctacca gactcccttt tgcattctgg ataatatgcc
    22141 gaatccgtcg cttaatttga tttctagtga cggcattccc cagttttttg ctaactgata
    22201 gacctactcg aaaatggttt ttctggtttt ctaattggta gaccacaaat ttgcgattag
    22261 caaaacttgt cccctccttg aaaatcgcct taaaatcttt ctctcttttt acacgaaagt
    22321 ttttcttcaa aactcaactc catctattaa attactacta ttataccata tttttcaaaa
    22381 aagccaatca tagcaaggtt ttggacaatt tcatcagaaa aagaagctgg aaaaataact
    22441 ttcccaactc cttgtattga atattttttt agtttcactt attttttcaa gcgttcaacg
    22501 tcacgggcaa tgactaattc ttcatctgtt ggaataacca agacacggat tttagctgcc
    22561 tctgttgaga tgtctcctgt aacgccaaag acattctttt catcatcaac atcacaacca
    22621 aaccacgaaa tccctgagat tacatcacga cggaaactct ctgcattttc accgacacct
    22681 gctgtgaaaa caatggcatc tgctccattt agcactgcaa ggtactgacc gatatgtttt
    22741 tggatacggt caacatacat ttcataagcc aagctagcct cgtgattccc ttctgctaca
    22801 gctgcttcta tatcgcgcat atcgctagaa ttagcagaaa ctcccaaaag acctgattca
    22861 cggttaagaa cacgactgat atcttctggt gtgttaaaat cctctgtata ttgcattaaa
    22921 taaggaatga tagctggatc aatatcccct gtacgcgttc ccatcataat accaccaaga
    22981 ggagtgaacc ccatagaagt gtctacagat ttgccggctt tcacagctgt aattgagcct
    23041 ccgttaccaa tatgacaggt aattaacttc aagtcttcta atggacgtcc caagagtttt
    23101 gcagcttctc ctgctacaaa ctggtgactt gtaccatgag caccgtattt acgaaccttg
    23161 ttttctgtgt aatattttgt tggtagaggg tagcgataag ctttctctgg catacttgtg
    23221 tggaaggaag tatcaaaaac aactacactg gtaatgtctg gcaacaattc cttgaaggca
    23281 cgaacacctg ctgcatttgc cgggttgtgt agaggagcca acaaactcaa ctcttcaact
    23341 ttttctaaaa catctccctc aacaactgtt gattctttga aatattctcc accagcaaca
    23401 acacgatgtc caacacctgt aatctcgtca taagccttga taatatcgaa acgaatcaag
    23461 tcatccaata aaattttaac agcttgtgta tgattttcaa tatccaaaat ttgttgttca
    23521 gaacggccgt caaattttac agttgaaatt gaatctttca aaccgatacg ttcaatcaaa
    23581 cctttcgcca atactttttc ttctggcatt aagtataatt gccatttcaa acttgaactt
    23641 cctgcattga ttgcaattgt ttttgtcata acatgatacc cctttttaag cgttttcaac
    23701 tattataaca aaatctattt tatatttcag taccttgagt ccatttttga aaattttctt
    23761 taaatttcat taaaacactt gcatcttgca agctagcaag tggataaaca aaaggctcta
    23821 ctgctatttc acttttcttc tgtaggataa aaatagtctt agattgttta gcattagcaa
    23881 agagattttc aggtaaacta atcatagcaa ccagacttgc ctcctctttc aaccagactt
    23941 tcaacaaatt actttgagga ctggtcaaca aatcactcgg agctagaaaa atagcgtatc
    24001 cgtctgactt gaggtactta agtccttgtt ccatgagcaa gtgatgggtg taagtatgtt
    24061 cttgactaga agcaacttga tggcgcgacg caacggcatc atcaggataa tagccaacag
    24121 gcaagtcgct gatgaccaca tcactttctt tgagcatttg tggacgaacg gcatctcctt
    24181 ggacaaagcc agcctgcaaa ccaattacat ctgccatgct agctgccaaa tcaatcagca
    24241 aatcatccac ttccattccc aaataatcca cctttttagt aagcgaggtc aagaaagtag
    24301 cgcccaaaat tcccatccca gaacccattt cgaggatagt aatttcctcc tctttaaaca
    24361 gctcttccac aataaacacc aaaagtaaag caatagcatc cggtgtaaac tggtgattgg
    24421 cctgcaaggg ttctgtttgc ccagccttca tcaagagaaa ctggtaggtc ttgagccatt
    24481 cttctttgcg tagtgctaaa cgcttaaggg tctgattgtt gtctttgacc tgttctagct
    24541 cagtctcacc atccagatag atgctgtttt gctccaccaa ggcgtcataa aagttggtcg
    24601 ccaaatcact ttggatgact tggacattct ctagtaaata ggtataagct tgttcaattt
    24661 tttcaaaatc catattccta tcttatcaaa tttcccttct tttttccatt cttatagaaa
    24721 aatcactccc tgactaggcg agtgattttt gggctactat taaaaagaat tctgagtgat
    24781 ttccttgaaa caggtcttca gcgctataga gaatctgact atgcacagat gaaaaaccat
    24841 gctcaattag cttgttttcc agttcagcta aatcaaatcc atgatgatta gcttctgtct
    24901 tggtaaaatc agcaatgatg agtttcccat cttccttcaa atgttgatga aacagtgaga
    24961 gagccgcatc caaatcaggc atatgatgaa gaacccgact aacagcaagg caatcaaact
    25021 ctttctccaa gggatttttc ggtaaatctt gctccaaaaa ctggatattc ttgattgctt
    25081 gctgctccac tttcaaacga gcttgctcca acattttctc agaaatgtct acaagagtga
    25141 ctgacttagc ctgcttggct aggggttagg ggcaaggcta atagacccgt gccaccaccg
    25201 aaatctaaaa tttctttgta tgatagaaga tcaatctgtt tctcggctgc ttgacatacc
    25261 aagtttgcga ggaagatatt tttagggaaa tcgaaagttt ctgctttgtg gttaaaatca
    25321 tgtttcatat ctttaattta gcacaaagta tagtaaactg aaataagata taaacaaatg
    25381 aattggagct taacatccat ttccagcaat tttctagaaa ctacagtgga ctattctgga
    25441 ttcaacgcat tatagttgaa ttgactagga attctctttc ttttcaggct tctcttctga
    25501 ttttttcttc tccacttttt cgcttgaatc ggtcgctacc tcttcctttt tatctctttt
    25561 ctcttctttg attttgactg aaggaaacag aaactcatat tggctcttat ccgtacgaac
    25621 cctcgtcact aagccagttt tcttgttttg atagcttacc tgacctagat taaaaacctg
    25681 ttccccactt tcttgctcaa ccttatcttt ggttcgttta gccatagcaa aagcaaccaa
    25741 tttttcttta ttcaaagcat agtcttgata gtgggcgact tgtcggttca aataaaattg
    25801 caacaaaaga ctaaagatgg ctgctatggt gactgcgtag aggagaacac ctgccttaac
    25861 ttttttcttt ttccacacga tagatgaact ccctttctaa gcctttttga aactggaaac
    25921 gaaaatgaac cagttgattg tcctctgtaa tctgagctga tttgaggcca taaaccatag
    25981 gctgataacc tcgaccacga gcattcgttt tacgaaaatc gtctgacttt gacttaccga
    26041 tggcgatgtc cttgccatct tgcttcatgt ataggcgatt gccttctact ttttcgaact
    26101 gcgaacggtc taattctgcc tcaagttggt ccacaaacaa gagccactcc ttttgctcgc
    26161 tttgttgctg gtagcgaact tctgaaatga ggagctgact catagtttga aaaaggagta
    26221 atcccccact gatgacaatg agggcaagta gggattctaa caaggtaaaa gccttgacct
    26281 tatggctctt tgattgccaa caactgttct gaaccatggt agacctccaa tcctttttca
    26341 ctagaaaata cctgaatctc aactccgttg atgcttacct gattttgccc cgtctgcagg
    26401 gccatcttag ctaccctcaa gacttcttcc ttttgcaaga tttttgcttc ctcttgccta
    26461 tttttttgaa tttgtcccaa aaggagggtc gcaatgctgg caaagatagc tagagcgact
    26521 actgcttcca gtaaaatcac tgccctaatt ttttgtttcc ttaatgcgtt taatttttcc
    26581 atttcctaga tataattgat agcgaatcgc tcctttactg gtctgaaatt caaccttagc
    26641 cagggacgaa ttgcccccag ctcggtcaaa tgtaatactt tggcctgatg gtgcctgaat
    26701 tcctttagga actgtcaact tttgactgcc attgctaagc gtctgcccat ctaaatttag
    26761 attagtcttt tgctgactgg ctacactgcg tttttgggtt tcccgataga gttcttcaaa
    26821 ctccataaag aaaatctgtt cctctaccgc cgcaaaagtg gactgaacag agccggacaa
    26881 gcccaaggca aggatactca caagacccaa aaccaagaga ctttccagca tggtaaaggc
    26941 cttaatcatt gactttacga tttgctccca catttttatc attgtattct ttataagctt
    27001 tagcctgttc ttccgtgatg cgtccatctt cttttaactt gcttaggcta gcatcttcat
    27061 tcttttccaa gctataaagt tctgcctggc tttccaccac cttaacaaca gctgcttttc
    27121 ctttgtcatt gactgcttct ttttgcttgg tcagattagg tacaaagagc aagaaaagca
    27181 cgctgataat cagcaagacc accaacatct ccaccaatgt aaaagcttta accttagctt
    27241 ttttcaagaa tgtcatcatt tttttcattt taaaaattta cctccatatt ttgatacatg
    27301 ggcatgagca ttgccgcata aagtaaaacg ataatcagtg ccacaaagat aaaaaccagt
    27361 ggctgcacca aattcatggt gcggttgact cgggtaaaaa aggcttccca agttttttca
    27421 gcatagattt ccaactcact acccagcttg gacttaactt ccccatactc tatgatgaga
    27481 ctcaattcct tcctaaagaa aggataggtt cctatcgtct gagaaaattc acggccattt
    27541 ttcagggttt gagccagatc ttgaccgatt tctttaaaga gctgggaacc ttgttcctgc
    27601 atcatttgaa aaatctgcgt cagctccatt ccctgtgaaa tcatattccc ccattcacgt
    27661 gcataatagg ctgtcaagta ggtctgcaca aagattccaa taaagggaag gcgtgctaag
    27721 atagaaaaga cactcatctt agaacttctt ttataaaaag ttagtgctaa aagggcaagc
    27781 acggaaacaa gccctaccat gactagaaaa atttgtggca gattaccgat aattcgggtg
    27841 gcaatattgc tactatccag ttgtgggagc aggtaattcc gtagccccag cataattaag
    27901 agaagaaaac ccagcaaaat caaaggatag gtcgctactt caattaactt tttcttgacc
    27961 ttggccagat tgtctagata ttcttctatc tttcccaaac tcaggtgaag attgccatga
    28021 acttcagcta gagataactg agtgacaatg gtacttgaac atcccaaact ttccatcatt
    28081 tctgagaatg atttcccctg agacaagcct gtacgcatct gggtcacaca ctgcttgtcc
    28141 aacaaggaac tcctatctaa aaaggagata gtctccacca gatgaaaacc gctagaaaag
    28201 agattgttaa atagggtgat gatatttttt tgcttagctg tagctaattt tttccgtctc
    28261 agcctgaaga cttgtgatat gtccatcttt aagaagctgg tcaatttgct cattccactt
    28321 ggctgcttgg tgttcttgat aatctctgtt tgcaaagtca acgattcctc ctcccccgat
    28381 taatctctgg tagcagactc cttgcagaac aactgccaat tcttcttcac tcacacccaa
    28441 ctccagcaga cgctcataaa cacctcggat actcttggcg tgaatggttg aaaagactgt
    28501 cgcacctgtc aaactagctc tgaccactgc acgcgccgtc tcgatgtcac gaatttctcc
    28561 gataatcaag agatctggtc gatgacgcaa ggaaagtttg attagatttt cataggttag
    28621 accgattgct tcgttcaact gcaactgaag catgtcgtcc tgcttgattt cgacaggatc
    28681 ttcgatggac ataacttgct gtcctttaaa gagtgacttg gacaattcat gcatcaaggt
    28741 cgtcttacca ctcccaaccg gaccagcaaa aagatagagt ccccgttgcc tgtactgctt
    28801 gcctaattct tcaatatcct gaaaccaaaa atgcaagtcc tgctcctcat cgtgcaacaa
    28861 acggataacc aaactctcat gcccccgata atcgcctaca gtagataaac gtagagacgc
    28921 catcttctgg tcataggcat aatcacagga acccagttga ctacgtcttt tttctcccac
    28981 attcataccc gccacaaact taaagtgact gataacggct gcaaactttt caaaatcata
    29041 gctaccaatt ttacagcgct cgtctcctac cctcatatga agctcatagg cgtctaactt
    29101 ggggacaaaa taaatgtctt gcgccccttt tttccgagcc gagcgaatga tttcttgtgc
    29161 aatttcttga accatactta cctcctcacc tatactattc gcaaagcgtt agaaaaataa
    29221 aaaaagcaac tccaaaaaag ttacttttct ctattttaat ttcatacggc aagaacgatt
    29281 cctttcctga ctggtttgtt tttcatagcc tgagcgtagt ttttcgtctg ggataacctg
    29341 ctcatcctgc aaaagagcca aagaatggta ttgttgtaaa tactcatcac attcatcaca
    29401 ccagcgttgg tcttccgggt cccaaaaaat ggtcatcaag tcgcttctac ttttataaag
    29461 aggggattct tgttccaatc taaaccagca agttttgtag tatttgagag catcataata
    29521 ctggtcaaat tttctacttg ctacaatatc ttcttcccaa ccttctaaga accaccacgg
    29581 ttcaaaatct ccgtacattt ctataacacg atacatattc attcattcct ttgtaccgta
    29641 tattataacc aattccgcca gacaaggaaa gaaatatgct catttcttaa ttttttgata
    29701 aaaaatccag ccatctgatg gatgactgaa tttctattta cttatgtaca aaaatatttt
    29761 tactacttag gcttctgaga tatcgttttc gataatgacc ttaatggcat ggtggtctgc
    29821 tgccttactg aagacttcgt aggctttttc aatttcactg agtttgaaat agtgagttac
    29881 caatttttct ggttcaatct tatgactttc aagcgctttc aacaattgtg gagtcgtatt
    29941 tgtagatacc aaaccagttg ttacattgat gttgcgaatc caaagtttat ctaaatcgaa
    30001 ttcaactggt ttaccatgca caccacagtt ggcaaccgtt ccgtctacac cgataatctt
    30061 ttgacagaaa tcaaatgttg caggaatacc aacagcttcg atagcgacat ccacaccacg
    30121 accatctgtc aaatcataaa tttctttaat ggctttttca gggtctgaag aattaacctt
    30181 atgagtcgca ccgaatgata gggcagtttc caagcggtta tcgtctaggt ctaccataat
    30241 caatttagct ggtgaataga attgggctgt taaaagagca gccaatccaa ctggacctga
    30301 accaataatg gctacgctgc aaccaggttc tactttccct tttaagacac caatttcata
    30361 tccagtaggc agaatgtctg acagcataac caaagcttca tctgacaagt cttctggagt
    30421 atggtaaaga gtattatctg catgagggac acgtagatat tcagcctgca taccatcaat
    30481 caagtgaccg aaaatccagc ccccttcgtc ttcacagtga gcataaattc cttttttaca
    30541 gtagtagcat ttaccacagg cacagacgca agaaatcaag accttgtcac cttttttgaa
    30601 gttggaaact ccttccccaa cttcttcaac aatcccaatc ccttcgtggc caagaatggt
    30661 accactttgg caagtaggaa catccccttt gataatatgg aggtctgttc cacaaatagt
    30721 ggtttttaca atacgcacaa tagcgtctgt tggcttgcga ataactggtt tgtctacatc
    30781 aacaaaagaa gcaagtcctg gtttaacata agtataggct ttcataaagc actcctccgt
    30841 ttttttattt gtaatattta tagttcaatt gtaagccttt ttctttgttt cttcaagctt
    30901 tttttgtgat ttttttaact ttttatttac cagtaaagta ccctgcataa aaaagcagaa
    30961 gttagtctct aacttctgct ttctctctta tgcttgataa cgttttacgc catctaggta
    31021 ggttgctacc aattccaaat ctttatctaa tacgatgaag tcagcgtcgt agccttcacg
    31081 gatttggcca cagacgtcat cgatgtgaac ggatttagct gggttgaagc tggccatcat
    31141 gactgcttca tgcggattcg caattcccca ttcgaccaca ttcttcaaac catctttgag
    31201 tttgaggata gaacctgcca agttacctgt cgatttgagg cgtgcagttc catttgcaac
    31261 gacaactggg aattctccca acatataatc tccgtcttcc aatcccccag ctgtcataca
    31321 gtctgtgata agagcgatgt tttctgttcc tttttgtttg ataagaattt cgcaagcctt
    31381 tggatctacg tggtgaccat cacagatcaa ctctgcatag gtatgtggca attggtacat
    31441 ggctccaacc atacccaatt cacggtgagt caacccacgc attccattgt aggcatgcac
    31501 ccaaacactc gctccagcat cgactgcttt tttggcttca tcaaaagtcg cgtttgaatg
    31561 tccaagagca accgtcacac cttcgcccgt aactgtacga acaaagtctt ccaccccatc
    31621 acgttctggt gcaagggcaa ttttattaag caagccattt gccgcttttt gccaagaatg
    31681 aaactcctca acacccgggt ctctcatata agttggattt tgtgccccct taaaagtttc
    31741 tgtgaaatat ggaccttcat aataaatccc acgaatctta gcacctgttg cttctttata
    31801 atggtttcca agattttcag tgactgcaag caattgctca taagtggctg ttaaagttgt
    31861 gggtaagaaa ctggtaacac cggtactaag aagtccttca ctcatagtat gcaatgtacc
    31921 ttcaatgttg ttgtccatca catctacacc tgcatatcca tgaatatgag tatccacaag
    31981 acctggggca atgctataac ctgtatagtc aatcacctca gccccttcag gaatctgctc
    32041 tacatgtttc ccaaacttgc cgtccacaag ttccaagtaa ccacctcgac gaactccgtg
    32101 tgggtagaaa aactgatccg ctttaatata gttaggcata atgttaacct ccttaaaaga
    32161 ttgattctac aatttattat gtcaattaca ttataaacct ttctttttta tttgtaaatg
    32221 gtatagacct attttttgga gatattttaa aagagctgga tttctccaac tcttctcttt
    32281 tttaatacaa gttattttga tttaaccggc ttgtcttgag ctgtctgcaa agctgtggca
    32341 atcgtatctg catacaattt tgctcctgct tcgatagtgc tactctcact cccgaaatga
    32401 acctggtctg ttcctgccca aatttctgga tgctctttcg caacttgatt ccaatctgct
    32461 atcgtaatgt aaggtgtctt ctctgccaat tctctcatat aggcagcagc cttctcaacg
    32521 atggcatagg tctcttttgt cttatctccc tcataaggag tcaccaaaat catatggtgt
    32581 cccttaggaa gatttttcac gatactgtcc cagtcatcct tgtaattctc aggattattt
    32641 accccagtcg caatgaccac cgtcttaggt aaaaatttat tctggctatt atttagcatg
    32701 atttcatttg cggtcttggt tgttacgctg acctgcgcgt taatctgtgc tccaggaaga
    32761 gctgtctgta gtgctgtatt tgcccttaaa gccactgagt caccaattaa catagtgcca
    32821 tcagcaattc ccaaactgtt tgcatctgcc cgttctgcca tcaccttggt ctggccaata
    32881 tttgttgcag cttgcttcaa gccattgaca gtcaagtctg tctcaaacgc tcccacttgt
    32941 ggtgccaaca aggtcaccgt gcagacaatg atggtcaaga ttcctgtacc tgctgcaaga
    33001 attgcgtgaa tataaggcag gggacgaagg gtttggacaa taggtgtgtt cttgcctgca
    33061 atccaaggtt ccaatacata aaatgacaga ctggcaaagc cataagaaca aatcagagtc
    33121 agtaatacag caagaagatt tgatgtcaac tgtgagaaaa tgatatagaa aggccaatgg
    33181 aaaagataaa ccgcatagct agtatccgct aaaaagctga taatcttcgg ctcctgtata
    33241 tgatgtgtct tttcatgtaa gacacgcgcc gccagaatca tggcaagagc tgcaagactg
    33301 gcaagtaaga agccgataag ataggcaaaa agataagtga atttgacaaa gaaagtcaaa
    33361 agaactagga aaccaaagcc tcctccaaaa actaccaaag tctttcgtaa atcccagatt
    33421 ttatccaact gcttgacgag ggaagtcgtc tgacgaacgc ctacaatagt tgctaacata
    33481 cttcccaaaa agaatggata gacatgagtt aaactggaga aataaacaga ggaataagag
    33541 gtcactagaa aactaccaat aaacatggag aagaaactga tcaagaaggc aacagcagat
    33601 aagagaaaga ccatcccctt caactgacca tttgatttag cttgtttgga taagaaccaa
    33661 actgccaatc cccaaagaat atagtagtga acctcaactg ccaaactcca attatgaaca
    33721 aacaaatgag gaatgaactg agattcataa ctcccacctg ttaggagttc atagaagttg
    33781 gtcataaagc ctaagacgct cgcaatctgg ccaccaattc cagcaacata gtcttggcga
    33841 accaagaaag taaaaggcat ggtcaccaag accatcaaaa ccacaggtgg cacaatccga
    33901 taaaagcgtc ttctaaaaaa tcctatcaaa tcaatctcat ggtttttaga aaattcttca
    33961 atgagtagag ctgtaatcag gaaacctgaa aatgtgaaaa agacatctac cccgaaaaat
    34021 cctccaggaa agatcgtctg aaagaagtga tacaagagta ccaaaagtaa acctgtaatc
    34081 ctaaccaagg aaaaccattt aatgcgcata cgagtttatt cttcctttca ttgtacactt
    34141 tattctatca aaaaaagaag aaaaatccta agagaaaact taggattttt agcttatatc
    34201 aattccattt tagaaattac gtcctgactt attatagcca tatttttcca caaaatactc
    34261 acggaattcc aagagattgt catccatgat ggcttgtcgc acttgcttca tcaggttaag
    34321 caagaagtaa agattgtggt agctagtcaa gcggatccca aaggtttcat cagccttgag
    34381 caggtgacga aggtaagcgc gtgtatagtt attacatgta tagcaatcac actcaggatc
    34441 cagtggcgta aagtcctcag caaactgggc atttttcaca accaaacgtc cttgactggt
    34501 catacaagtc ccgttacgag caattcgagt cggtaagaca cagtcaaaca tatccacccc
    34561 acgaatgacc ccatcgatca agctatctgg cgctcccaca cccatcagat aacgaggttt
    34621 attttcaggc agcagttgag ttgtaaagtc caagaccgca ttcatctctt catgggtttc
    34681 tcccactgcc aaaccaccga tagagtagcc tgagaaatcc atgctgacaa gatcatgagc
    34741 tgattggcgg cgaaggtctt caaatcctgc accttgcaca attccaaaca aaccttggtc
    34801 atgtggacga cggtgagcct tcaaaccacg ctcagcccaa cggctggtac gctctatcga
    34861 tttcttaacg tagtcataag gttgataaaa ctgaggacat tcatcaaagg acatcatgat
    34921 gtctgaaccc agattattct gaatagagat ggctttttct ggggatagga acatcttaga
    34981 accatttaga tgatttttaa aggttactcc ttcttctgtg atattacggc tatctgctaa
    35041 agaataaacc tgaaaaccac cactatctgt caagataggc tggtcccaat tcatgaactt
    35101 gtggagacca ccagcgcgtg caatgagttc atctccaggg cgaagccaga gatgataggt
    35161 gtttgatagg ataattcccg aacccatctc cttcaattct tcaggtgact gagttttgac
    35221 agtggcttgt gtcccaactg gcataaacat aggtgtcgga aaggtaccgt ggggagtgat
    35281 gatttctcct agacgagctc ctgtgtgttt ttctttctta atcaaacgat atttgattgg
    35341 tgaatctgac attttttacc tccgaagctg ggaaagacag tcccagttca tacagtgtgc
    35401 ccaaggacac actgtatgat tttatcaaaa aaagtaggaa ctgtcacgaa ctatgtataa
    35461 gaactatggt ataataagag aatgaactat ccaaaaattg atttaaaaac aattcgtcag
    35521 gagtcaaaac attttcaggc tgacaccccc cgtctctttc tcctctatat ccttcccagt
    35581 atgctggtca tcttatctag ttttcttaat cccttatctc ggatccacgg gactgtttta
    35641 gaacagccat ttttcagcat acttggccaa atacttcaag cctacctctt tcctctacta
    35701 gtctccttta tcgggactat tcttttgacc agttccgtct atgcaacact gacactcatg
    35761 aaagattcta aaacggagcc atctgtaaaa aacagcctag ctctctttga tgaggagcgc
    35821 ttttctcaaa cctttttgac cctacttctc aaacgtttct atctcttttt atggagtatc
    35881 cctaatttac ttgggattca cctcctcttt tatagtagct ttttagccaa aaaattcgtc
    35941 accctacatc ctgagtttcc aagtctagat ctctcatcag ttgaaaccga gcgtttcctc
    36001 atggtctttg gactctactt tcttgcaagt ctcatcttga tcattgtagg aaatatcctc
    36061 tatattccac agtattatgc ctattcacag gtggaatttc ttctctgtta tagtctagac
    36121 ctaggacagg tcccaccaag acgaatccta aaaactagcc gttcctttat gaagggctac
    36181 aagttccagc actttgtcct agatttacag cttcttcctt ggtacttcct caattggatt
    36241 acctttggca ttgctagttt ttcactccta ccttatatcc aatgcactaa aattatgttt
    36301 taccgagcag tattggctcg aaaacgtcca aaagcttgaa ggtttttccc ctcaagcttt
    36361 tttctatcaa tgagtttctc cgcctaccaa cttgagttcc tgatctccat gttctatcat
    36421 agcgccgatt gctgcttcta tccctcgccg aatatccact aaactcatag ctggagtagt
    36481 ctgtctgttc accacctgtt ccatcatata aggaatatgc ataaaacctg ccttaacata
    36541 tggaaatttc ttttctacca aatagagagc ctgatacatc aaatggctgc agacaaaagt
    36601 ccctgccgta ttggaaacag aggccggtaa gccctctttt tttatagctt gaaccatcgc
    36661 tttaatcggc aaactactaa agtaggccga agcaccatct aggcgaatgg gacggtcaat
    36721 cggttgatta tcttcgttat cagaagtacg tgcatcgtct tgattaatgg ccactcgttc
    36781 aggtgtcaaa ctagttcttc caccagcttg cccaatacaa aggacaaagt caggttgata
    36841 acgattcatc tcttcttcca atacttgagc agatttgtga aaaactgtcg gcacctctag
    36901 ccagcggacc tcagcaccat ggatttcagc tggtaaacct ttaatggtct ccaaagttgg
    36961 attgcccttt tcccctccaa agggctcaaa acctgtcact aatactttca tttctaactc
    37021 ctcaaaacca attcaatgag acttttttct taaaaacaag tataacatat ttcccttatt
    37081 ctggattaaa aaggattagc gagattagaa tcgaccttca agtgtattta aagagtaaaa
    37141 tactaatcaa ggccaaaata gctgatccac cttgtttcaa aataattttt ttatccgctg
    37201 ttaaagagcc gtaagtcgca gcaccaatca caaataagac aaaaatagtc acaatttcta
    37261 aattctgtga gaaataaatg acatacaaga gaaagactcc tagcagagcc ttataaatcc
    37321 cttgattttt gaacaatgaa cttactgacg gatgagccaa ttcttccttt tccatattaa
    37381 atacacgact agtcgcatct gattgcgtgg caatactttc caaataaaaa atgtaaaaat
    37441 gctccaaagc aacgatcgtt gttaaaataa ttgacatcta tttctcctta aatttctata
    37501 ttttcaatac ccaaaaccaa tttatttaat aaacggctga gttctgttct ctcaaactct
    37561 gttaagatac tttccatctc ttccttaacc ttgatatgtt gcagaggggg attcaccact
    37621 aactgctcct tggcatactt cgcagcctct accaacactt cccgctgatt ttcaggatta
    37681 cgatgacgct ccaccaaacc ttccgtttcc aaaatcttga aatgccgtgt caaagcagcc
    37741 tgatcaattt tcaaacgctc ctgaaccgcc atttggttac aaagagaatg ctccagcaaa
    37801 aacagtaaaa tctgataccg tgtcaaacta atcccaagcc tttttcaaac aattgcgtac
    37861 tcgcttgctc agacaagcgg agttgataca gtaaatcttg aatctctagc atttcttccc
    37921 ctctcttttt attgatttat caatagttga ccagtcaatt ataatataaa gtatagtgaa
    37981 atgaaataag agcaggacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
    38041 gaagcagtta tgtactattc tagtttcaat ctactataaa atagattgca agaatgttgc
    38101 ttggattatt acaagataat aactttccat tttccattca aattgatact caatgaaaat
    38161 caaagagcaa actaggaagc tagtcgcagg ttgcccaaag gacagctttg aggtttcaga
    38221 taaaactgac gtggtttgaa gagattttcg aagagtatga caaagaaaaa acgaacgaga
    38281 atcgcttcct atcacgaaag ctagatctca ttcgtcaaaa tgatgttacc aagtcgggtt
    38341 tatccattca ttgaaacgtg aacgtggtca tagtgatttt ctgtcacact accacggtct
    38401 ggcattggat tccaagtgtt agctggccca tatttgctat cgaatggagc atagaaacgt
    38461 tgtttccaga tgatgtaact aatgccacgg ctggccatgt tttgaatagc atattccgca
    38521 atcttatccc ctaattctga acgttctggt accataaagt cgatagccaa accttttccg
    38581 tgatctccac tgtctcctgg acgataacca ctaaaggatg taatgccaaa caagttagca
    38641 atttcttctt taaaggcagc tgtttgtggt tgaagacctg cattttcaga ttttgctact
    38701 gcaagtccag cataatcggg cgcagctgga gcctcgtaag ttgttgaaat tactttcgtc
    38761 tcttctggtt gataagtaga aactgctgct gtagctccat ttgatgatgc tacttctttt
    38821 gcttctgaac ttgttgtagt tacttccact gctgatgcag cttgagggct tgcttgtgtt
    38881 tcctgcttct cagctggagt cgtttcctga ggagttgctt cttcaactgg gcgagttgtt
    38941 tcggtcgttt gctcctctgg gacagaagtg cccgtagatg gtgccacttc ttcagaagca
    39001 atcactgtct ttgtaacttc tgcaattggt tgagaaaggt ctgcaacctg aacagtttga
    39061 tcatcaacgg tcacttgatt agtggtcaaa tctgctgtcg cagttgtcac ttcttcacta
    39121 gagtctgctt gaggtgtttg gatttcaact tctgttactt cttctgcttc attgacagtc
    39181 gttgtcaaaa cagtttctgg gaaaatcaag tccatattag tgattttgtt cagattcgca
    39241 agcactgtga catctacacc caaggcttct gcaatggtgc tcaaagtatc accatactgt
    39301 acggtataac ttgttttgtt gtccgtttta gtcaaatcgt tttggatttg ctcaacacta
    39361 cgtgcagtcc aaagaacttc ttctgcttga gttgccaata ctggggcaaa tgacaaggct
    39421 actgttgacg ctaataacat tcttttcttc attctttcaa attcctttca aatgagtacc
    39481 tgtctatcat aacacaaaaa atgtagaaaa aaagccctct cgggcctatt taacagaact
    39541 gtctattcct tgtaacaaac taggttactt gaaatagttt gttaggtctc tgtcacaaaa
    39601 ctgacacaat ctttttatca cttagtctca aaaatacggg aaatatctgc taacgcttga
    39661 atcctgtgat tcccttcata agtagactct aaaaagttga tgctttgaat cccactattc
    39721 tgggcaaatt ccacatccag agtccgatcc cctatataat aagtattatc agaattcaac
    39781 tgatacttat ctagcagata ggtagccgct tctggacttg gcttccgcac aaagccactc
    39841 tgactggtta aaatctctgt aaaataggat tccaccccca agtccttgag aatggtaaaa
    39901 gcgttgttcc ccttatgagt atatataaac tgctgaattc ctgattcgtc tgcccaagct
    39961 agcacctcac gcgcacctgg catcaaaact acctgagcat tcttctcagc cagactctgg
    40021 gcacgcacct gatttagcac ctcaacatcc agatttctat cttctgccac ccgcacaagc
    40081 aaatcttgca ccgaatactt gaagataaac tctctcacct tctccttatc ataaggaata
    40141 gaaaactgag caaaagtctc ctcaatccct gataaaatcg cttcgtaaga gtccaataaa
    40201 gtcccgtcta aatcccaaat aaaagctgtt ttttgcattt cctatccttt caagctcata
    40261 tagcgctggt aacgatagcg tagaaataac cagcgaaaac cattatccaa gagagaccct
    40321 gcccaaatac caggcaagcc ccaaccaagc acaatcccca tcagatatcc tgtcccaatg
    40381 cggatacacc acattcctat acttgtcgca taaaaaggga ggcgtgcatt tcctaatccc
    40441 tgccagactg ccgtatagat gactgttcct gtcgtcatag gggtcccaag tagtgaaaac
    40501 agtgtcacta gaacactagc ctccaccgct agagaatcag tcgtatagag atgagttaat
    40561 ggtacaccca agacatatat actaaaggac aggggcaaca tgaggaacag agaaagccaa
    40621 aaggtttgtt tactcaaact agcaactctt ttccaatcat cctctccaac tgctcgggcc
    40681 aacagcatga ccgttgccgt agcgacgcca aaggcaggca tatagttaaa ctgggtcaag
    40741 acttctccga ttgcattccc agcaactgcc tccgtcccaa aagaaacgac caaggcaatg
    40801 atcactacat ctccagccct catcataagt cgctctccag ctgctggtaa agccaaggtc
    40861 aacagttcct tatctaaacc aaaagttggc ttcccataag gcagttttaa ttgtgaccac
    40921 aaaatcacaa gaccaaccaa acgagacaca attgtccccc aagcaacacc agctatcccc
    40981 atatccagaa caaaaatagc tagacttgaa aaaagaatat tcaaggcatt ggataaaaaa
    41041 ctaacataga gaggcagacg tggattatgc gttgcacgaa tcaaggctcc tagactagtc
    41101 attaaaccta agagaacaat cgatccgcct accaaagata gatacagtcc accactctca
    41161 gctacatccc tctccgtccc caaaagtcct atcatctctt tcccagcgaa gatggacaaa
    41221 aatcctaaaa ggaaacttaa tagtaaggta atcttcaacg cctcagtcac atgataggcc
    41281 aacttcgact ggtctttctg ccctatgctt tttgaaataa cactggaaat agcagctccc
    41341 agagcgatga aaatcgcctg ataaatggtg ataatattac cagctactga aacccctgaa
    41401 atagctatca atcctaaatg agcaaccaaa taactgtcca ccattcccat tagcatctgc
    41461 aaaaagtttt cacccatagc tggcaatgca atattaagaa tgtctttatt tttcttaaac
    41521 aatctctcct cctgatgaaa agaaactcag ttggtttccc aaccgagttt gctccctctg
    41581 tcttaaagtc ctaaataagc ctcaaccgct gcttgcatgt cagcagctgc cactgttgtc
    41641 ttgtgacgaa ttggagctat ttcaagccca tcaactgctg gtggcactgc aacgcctgag
    41701 atttcatgta attgagccaa ggcttcaaag tctgttaaac ctgcttttcc agttacagct
    41761 tctactgcaa ctactgggaa cttgtatgga ctagctgttg aagcaatcac tgtcttagtt
    41821 acatctccag tggccgattg gtatttttta taaactgctg aagcaacagc tgtatgaggg
    41881 tcctcgatat aagaatctaa ctcacaaaca cgcttgatct ctgctgccgt ttcttcctca
    41941 gtcgcatatt cagctgcaaa gaggtccaaa atctctgcat caaagtctgt caacttatat
    42001 tgtccttgcg tgttcaaggc attcataagt tcagttgtct tttcagcatt atttcccaaa
    42061 agatggaaaa tcaagcgctc caagtttgaa gataccaaga tatccataga tgggctggtt
    42121 gttaccttaa actcacgttt tttgtcatag acacgtgttt taaagaagtc tgtcaaaaca
    42181 ttgttgtcat ttgaagcaca gattaattta ccaactggca aaccaatttg tttggcataa
    42241 aaggcagcca agatatttcc aaagtttcct gttggtactg tgaagttaac cttttcacca
    42301 gctacaattt caccagtctt aaccaattga gcgtaagcat aaacataata aacaatttgt
    42361 ggcaccagac gaccaatgtt catagagtta gctgatgaaa attgcaactt gttggtagtc
    42421 aatttttcac gaagagccac gtcgttaaac atgtgcttca catttgtttg cgcatcgtca
    42481 aagttaccat caatagcaat aacatgagta ttgtcgccag tttgagtggt catttgtaac
    42541 tcttgaatct tgctgacacc atcctttgga taaaagacga taatctcagt accaggcaca
    42601 tccgcaaacc ccgccatagc agctttcccc gtgtcaccag atgtcgctgt caagataaca
    42661 atcttgttct ccaaaccatg tttcttagca gcagtcgtca taaagtatgg caaaatagac
    42721 aaggccatat ccttaaaggc aatcgttgaa ccatggaaaa gttccaaatt gtattgccca
    42781 tctaatttca ctaatggtgc aatagctgga gtatcaaatt tgctatcgta ggcattgttg
    42841 atacagtagt ccaactcctc agctgtaaag tcatctaaaa atgctgacaa aactagctta
    42901 gcaacttcct ggtaagaagt atctttcaat ttgtcaaagt ccaaatctac ctttggataa
    42961 gtaaccggtg taaacaaacc gccgtccgtc gccaaacctt gcaaaattgc ttggctagca
    43021 gttactgtat tgttggcatc acgcgttgat tgataaacta atgtcattac tctctatcct
    43081 ttatctatag tctcgtccat tatatcatga tttttcagaa aattctccct ttataagaga
    43141 aattataggc tcaattcttc tctcaaaggc tgtaaatagg tcatttcttc cttatttctc
    43201 accactagtc cctcctcagc tagtaggagt aagtctttgg aaaattcaat aatcgtagtt
    43261 tcttcctcat ctgtaagatt tttcttagaa aattgtctcc ttaaagactt gtaatcataa
    43321 ccaaatactt taaagaaagg tgctgtttct aagtaagctt ctaacttgtc taaattaacc
    43381 aataatccca agtgaaaagc tgcagaagca aaagtcctat caagtggctg tgtacacaca
    43441 ctacgaaact caactgttcc tcgagtcgtt aagtcttggt actggtaact acgatgagtt
    43501 tcaaaatcct tctcttgggg ggaaataata acctcatccc cattcagagc aaatgcttgg
    43561 atttcaggtg tagccaaata gtccccagcc tgaataggat aaaaataata ggtctgccca
    43621 tcacgttccg cagtaaaaat cgcagaatga tttagatagt caaaaaaatc agcttcatca
    43681 ttaaggagtc tagcattgac cccaacattc tctggataga taccatgcat agattcttcc
    43741 cagaaaatat cccttgaaat tttcgtatcc caatccgcac ccgaaaattc agagtttgca
    43801 aataaataag ccttagccgc ttcaatttga gtaaaagcat taatcacccg taagtagttg
    43861 gtttttgaaa tatccagctg aacctggctc ccacagataa aagtaccata ttcagggaaa
    43921 tgatgtaaat ctgatttaat aatatttcta ctcaaattca aataatccat caacatctga
    43981 tagcgtggat aagccactgg acaattctca tttttatccc agttgggatg gataccacag
    44041 ccaacaatag catgatttga ttcagctaac tttctctgaa ttacattcat ataattattg
    44101 aaacgatttt cgacctcttg aatcgtttca gccttaccaa atgcaaactc aatcgttgta
    44161 taggaaactt caaataaaat agcatcctga cttatcggat ctactaactg gatcagattg
    44221 ccaaaatcat ctacctttgc tacggtgaga tccaaagtag aaactaaata atgaaataga
    44281 tcctttataa cttcaacatc tgtagcatcc ccttctaaac ttgcaacagg atactccaac
    44341 tcaattccga caaacaattc aggattttct ttaatatttt tcaaataccg atgttttaat
    44401 aaatcgatag aacaagacat actaccctac actttcttaa tctaaataaa atttgaataa
    44461 ctataattat accaaaaata atataaaaaa agaacgaaga ataacaacgt tcctaacact
    44521 a
//
LOCUS       DAVKWS010000010        42549 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKWS010000010 DAVKWS010000000
VERSION     DAVKWS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.010"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..213
                     /locus_tag="VW852_000607"
     CDS             <1..213
                     /locus_tag="VW852_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV0271791.1"
                     /translation="DVHCDAVMVKDVNEDLMKGYDIFTPIAATDLGFEPGIPVIEAGP
                     ILFRIPAMSAPVFDNIESAIKEHELS"
     gene            261..1736
                     /locus_tag="VW852_000608"
     CDS             261..1736
                     /locus_tag="VW852_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV0271792.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            1762..1839
                     /locus_tag="VW852_000609"
                     /pseudo
     CDS             1762..1839
                     /locus_tag="VW852_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2086..8787
                     /locus_tag="VW852_000610"
     CDS             2086..8787
                     /locus_tag="VW852_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV0271793.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TQKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESADGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9044..9892)
                     /locus_tag="VW852_000611"
                     /pseudo
     CDS             complement(9044..9892)
                     /locus_tag="VW852_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10103..10462)
                     /locus_tag="VW852_000612"
     CDS             complement(10103..10462)
                     /locus_tag="VW852_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV0271794.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLTLSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYVEMIVELFLMK"
     gene            10554..11510
                     /locus_tag="VW852_000613"
                     /pseudo
     CDS             10554..11510
                     /locus_tag="VW852_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11494..12051
                     /locus_tag="VW852_000614"
     CDS             11494..12051
                     /locus_tag="VW852_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0271795.1"
                     /translation="MKRLLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALKKTQERLHQAGLTNAQLILQGHETLDQFVTKTKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCHLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12044..12298
                     /locus_tag="VW852_000615"
     CDS             12044..12298
                     /locus_tag="VW852_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271796.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12310..14364
                     /locus_tag="VW852_000616"
     CDS             12310..14364
                     /locus_tag="VW852_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV0271797.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14543..15403
                     /locus_tag="VW852_000617"
     CDS             14543..15403
                     /locus_tag="VW852_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV0271798.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15614..16324
                     /gene="ccdA2"
                     /locus_tag="VW852_000618"
     CDS             15614..16324
                     /gene="ccdA2"
                     /locus_tag="VW852_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0271799.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16344..16910
                     /locus_tag="VW852_000619"
     CDS             16344..16910
                     /locus_tag="VW852_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV0271800.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16921..18033
                     /gene="msrB"
                     /locus_tag="VW852_000620"
     CDS             16921..18033
                     /gene="msrB"
                     /locus_tag="VW852_000620"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0271801.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18301..19038
                     /locus_tag="VW852_000621"
     CDS             18301..19038
                     /locus_tag="VW852_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0271802.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19035..20726
                     /locus_tag="VW852_000622"
     CDS             19035..20726
                     /locus_tag="VW852_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0271803.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20842..21405
                     /locus_tag="VW852_000623"
     CDS             20842..21405
                     /locus_tag="VW852_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV0271804.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21427..27039
                     /locus_tag="VW852_000624"
     CDS             21427..27039
                     /locus_tag="VW852_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0271805.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSITNQEQARTENQVVETEEAPKEEAPKTEESSKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEVVPDDTKYVDEEV
                     VERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPVVPKVVKKGTKPVEGTAVETR
                     EEVIPFATKEQEDDTLKRGTRQVAQEGVNGKKQITETYKTIRGEKTNEAPTVEETVLQ
                     APQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVEIKSIKVALKDNT
                     GTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETEMLEDKEVQLDLKKV
                     EIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKTTRLAVSSVEEVV
                     VDGKTLYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYSFNELITDMQKNP
                     TGTFKLGADLNAANTPTPSKSYVTGEFKGKLSSVDGQHYTIHNTARPLFNNIVGGTVK
                     NINLNNVNIDMPWADQIASVANVIKGGSLIENVKVKGNVLGRNWVSGFIDKIDSGGRL
                     VNVAFIGNVTSVGDGDKFLTGIVGENWKGYVEKAYVEANIKGKRAKAAGISYWSQNSG
                     DNNAVGRDGAIKKSVVKGSIDVEKPIEVGGAVGSLNYLGYIEDTVAMMKVKNGEIFYG
                     SHDIDTDPYYTGERVNRNFIVDGVSEGKSSYTYSKQQKRIKSISQEEADKKIKELGIT
                     ADKFTIIDPIVNKLNNIKQKADTYKDTQDYKVDRELAYRNIEKLQPFYNKEWIVDQGN
                     KVPSNSKLLTTEVLSVTGMKDGQFVTDLSEIDKIMIHYADGTKEEKSVSPKPTSNVEQ
                     VKEYGITDLGDVVYTPNMVVKDRAQLLSDVKAKLDTITLESPEVRAITGNVGALYLEE
                     SFAEVKGNLDKLVKALVENEDHQLNSDEAAIKAFLKKVEDNKAKIMMALTYLNRYYGI
                     EYGDLNIKNIMMFKPDFYGKTPSVIDRLIRIGSQEKNLKGDRTQDAYREIIAGDTGKG
                     DLRSFLDYNMRLFTNDTDLNDWFIHSAKNVYVVEPETTNPDFKNKRHRVFDGLNNNMH
                     ARMILPLLNLKKAHIFMISTYNTMAYSSFEKYGKNTEAEREAFKERINYVAKAQQTYL
                     DFWSRLALPNVRDRLLKSQNMVPTPVWDNQAYAGIKDANRRGYGTDGKVATPIRELFG
                     PTDRWHQINWNMGAMAKVYGKPYEDEQVFFMVTNMLEDFGISAFTHETTHINDRMAYL
                     GGHGHRPGTYLEAYAQGMLQTPDKSTSNGEYGALGINMAYHRQNDGNQWYNPDPDKLQ
                     SREQIDHYMKNYNDALMMLDHLEADAVLPQLNGNTSRWFKKMDRQYRSGNSGHQFDKI
                     RELTEDEKKVAINTIDDLITNNLITKHGAPGDGTYNPTDFRSAYVNMNMMTGIYGGNT
                     STGAPGAASFKHNTFRMWGYFGYENGFVGYASSKYQNAANKENNRMLGDDFIIKKVSN
                     GRFTNLEDWKKEYYKEVKAKGEKGFTAIEIDGKQITNYAELKVLFDKAVEADLAGGGT
                     AKTVELKSKVFKALLKNSDGFTGNLFKA"
     gene            27113..28834
                     /gene="pabB"
                     /locus_tag="VW852_000625"
     CDS             27113..28834
                     /gene="pabB"
                     /locus_tag="VW852_000625"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV0271806.1"
                     /translation="MHRKTVIDFRALGERHIFTQPIKELKTRDLTEVTALLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLNLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            28835..29452
                     /locus_tag="VW852_000626"
     CDS             28835..29452
                     /locus_tag="VW852_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0271807.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29502..30492
                     /locus_tag="VW852_000627"
                     /pseudo
     CDS             29502..30492
                     /locus_tag="VW852_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            30627..31586
                     /locus_tag="VW852_000628"
     CDS             30627..31586
                     /locus_tag="VW852_000628"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV0271808.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31683..32522
                     /locus_tag="VW852_000629"
     CDS             31683..32522
                     /locus_tag="VW852_000629"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV0271809.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLFKQLEANPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32570..32740)
                     /locus_tag="VW852_000630"
     CDS             complement(32570..32740)
                     /locus_tag="VW852_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV0271810.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            32861..33745
                     /gene="miaA"
                     /locus_tag="VW852_000631"
     CDS             32861..33745
                     /gene="miaA"
                     /locus_tag="VW852_000631"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV0271811.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33738..34976
                     /gene="hflX"
                     /locus_tag="VW852_000632"
     CDS             33738..34976
                     /gene="hflX"
                     /locus_tag="VW852_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV0271812.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            34969..35592
                     /locus_tag="VW852_000633"
     CDS             34969..35592
                     /locus_tag="VW852_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV0271813.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35607..36536
                     /locus_tag="VW852_000634"
     CDS             35607..36536
                     /locus_tag="VW852_000634"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV0271814.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAKKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36557..37312
                     /locus_tag="VW852_000635"
     CDS             36557..37312
                     /locus_tag="VW852_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV0271815.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37366..38274)
                     /locus_tag="VW852_000636"
     CDS             complement(37366..38274)
                     /locus_tag="VW852_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0271816.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38338..38607
                     /locus_tag="VW852_000637"
     CDS             38338..38607
                     /locus_tag="VW852_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV0271817.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38607..38987
                     /locus_tag="VW852_000638"
     CDS             38607..38987
                     /locus_tag="VW852_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0271818.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39076..39303
                     /locus_tag="VW852_000639"
     CDS             39076..39303
                     /locus_tag="VW852_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271819.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(39343..40068)
                     /locus_tag="VW852_000640"
     CDS             complement(39343..40068)
                     /locus_tag="VW852_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV0271820.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDSCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            40230..42071
                     /gene="typA"
                     /locus_tag="VW852_000641"
     CDS             40230..42071
                     /gene="typA"
                     /locus_tag="VW852_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV0271821.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42091..42345
                     /locus_tag="VW852_000642"
     CDS             42091..42345
                     /locus_tag="VW852_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV0271822.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        13087 a         7882 c         9480 g        12100 t
ORIGIN      
        1 gatgttcact gtgatgcagt catggtgaaa gatgtaaacg aagaccttat gaaaggttac
       61 gatatcttta caccaatcgc tgcaacagac cttggttttg aaccaggtat cccagttatc
      121 gaagctgggc caatcttatt ccgtatccca gcaatgagtg ctccagtatt tgacaatatt
      181 gaatcagcta ttaaagaaca cgaattaagc taattctaat ttgttaacat tcatataaca
      241 taaaaaaggg aggaactgtt atggatgcaa tctttgacct aatcggaaag gttttcaatc
      301 ccatcttaga aatgggtgga cctgtcatca tgttaatcat tttgacagta ttggctttac
      361 tttttggagt gaaattctcc aaagcgcttg aaggtggtat caaacttgcc atcgctctta
      421 caggtatcgg tgctatcatc ggtatgctaa atggtgcttt ctcagcatca cttgcaaaat
      481 tcgttgaaaa cactggtatc caattgagta ttaccgacgt tggttgggca ccactcgcaa
      541 caatcacttg gggttctgct tggacactat acttcttgct catcatgttg attgtcaacg
      601 tagtgatgct tgctatgaag aaaaccgata cacttgatgt cgatatcttt gatatctggc
      661 acttgtctat cacaggtctc ttgattaaat ggtatgctga taacaatggt gtgagtcaag
      721 gggtttcact ctttattgct acagcagcta tcgtccttgt cggtgtgttg aaaattatca
      781 actctgactt gatgaaacct acatttgatg accttcttaa cgccccaagt tcatcaccaa
      841 tgacatcaac tcacatgaac tacatgatga acccagttat catggttttg gataagattt
      901 ttgaaaaatt cttcccaggc cttgataaat atgactttga tgctgctaaa ttgaacaaga
      961 aaatcggttt ctggggatct aaattcttca tcggtttcat ccttggtatc gttatcggta
     1021 ttatgggaac tccacatcca attgcaggtg ttgcagatgc agataaatgg cgtcttgtta
     1081 tcaaaggatg gttgtctctt ggtttgactg ccggtgtatc tttggaactc ttctcactta
     1141 tcggttcatg gttcatcgca gccgtagaac cactatcaca aggtattaca aacgttgcta
     1201 ctaaacgtct tcaaggacgt aaattcaata tcggtcttga ctggccattc atcgctggtc
     1261 gtgctgaaat ctgggcttgt gccaacgtac ttgcaccaat catgttgatt gaagcagtgc
     1321 ttctttcaaa agttggaaat ggtatcttgc cacttgcagg tatcatcgct atgggtgtta
     1381 ctccagctct cttggttgta actcgtggta aattgctccg tatgattatc ttcggaacac
     1441 tcttgttgcc actcttcctt ctttcaggta cccttattgc accatttgca acagaacttg
     1501 ctaaaggtgt aggtgccttc ccagaaggtg tgagccaaac tcaattgatt actcactcta
     1561 ctcttgaagg accaatcgaa aaacttcttg gttggacaat tggtaacact acaactggtg
     1621 atatcaaagc aatccttggt gcagtagtct tccttgtatt ctatatcggt atctttgctt
     1681 ggtacagaaa acaaatgatc aaacgtaacg aagagtacgc agcaaaagca aaataatgcg
     1741 ctcctataaa atataaactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt
     1801 tgctcaaaac acagttttga ggttgtagat aagactgacg aagtcagctc aaaacacagt
     1861 tttgaggttg tagatgaaac tgacgaagtc agctcaaaac atggttttga ggttgtagat
     1921 gaaactgacg aagtcagtaa tcatatctat ggcaaggcga cactgacgaa gtttgaattg
     1981 gattttcgca gagtgtaaat tgttgaaact aggttgtcat accatgtata ccacttgggt
     2041 tttggcagta gctaactgca ctaaatataa tataaggagg gttttatggg gaaaggtcat
     2101 tggaatcgga aaagagttta tagcattcgt aagtttgctg tgggagcttg ctcagtaatg
     2161 attgggactt gtgcagttct attaggagga aatatagctg gagaatctgt agtttatgcg
     2221 gatgaaacac ttattactca tactgctgag aaacctaaag aggaaaaaat gatagtagaa
     2281 gaaaaggctg ataaagcttt ggaaactaaa aatgtagttg aaaggacaga acaaagtgaa
     2341 cctagttcaa ctgaggctat tgcatctgag aagaaagaag atgaagccgt aactcaaaaa
     2401 gaggaaaaag tgtctgctaa accggaagaa aaagctccaa ggatagaatc acaagcttca
     2461 agtcaagaaa aaccgctcaa ggaagatgct aaagctgtaa caaatgaaga agtgaatcaa
     2521 atgattgaag acaggaaagt ggattttaat caaaattggt actttaaact caatgcaaat
     2581 tctaaggaag ccattaaacc tgatgcagac gtatctacgt ggaaaaaatt agatttaccg
     2641 tatgactgga gtatctttaa cgatttcgat catgaatctc ctgcacaaaa tgaaggtgga
     2701 cagctcaacg gtggggaagc ttggtatcgc aagactttca aactagatga aaaagacctc
     2761 aagaaaaatg ttcgccttac ttttgatggt gtctacatgg attctcaagt ttatgtcaat
     2821 ggtcagttag tggggcatta tccaaatggt tataaccagt tctcatacga tatcaccaaa
     2881 taccttcaca aagatggtcg tgagaatgtg attgctgtcc atgcagtcaa caaacagcca
     2941 agtagccgtt ggtattcagg aagtggtatc tatcgtgatg tgactttaca agtgacagat
     3001 aaggtgcatg ttgagaaaaa tgggacaact attttaacac caaaacttga agaacaacaa
     3061 catggcaagg ttgaaactca tgtgaccagc aaaatcgtca atacggacga caaagaccat
     3121 gaacttgtag ccgaatatca aatcgttgaa cgaggtggtc atgctgtaac aggcttagtt
     3181 cgtacagcga gtcgtacctt aaaagcacat gaatcaacaa gcctagatgc gattttagaa
     3241 gttgaaagac caaaactctg gaccgtttta aatgacaaac ctgccttgta cgaattgatt
     3301 acgcgtgttt accgtgacgg tcaattggtt gatgctaaga aggatttgtt tggttaccgt
     3361 tactatcact ggactccaaa tgaaggtttc tctttgaatg gtgaacgtat taaattccat
     3421 ggagtatcct tgcaccacga ccatggggcg cttggagcag aagaaaatta taaagcagaa
     3481 taccgtcgtc tcaaacaaat gaaggagatg ggagttaact ccatccgtac aacccacaac
     3541 cctgctagtg agcaaacctt gcaaatcgca gcagaactag gtttactcgt tcaggaagag
     3601 gcctttgata cttggtatgg tggcaagaaa ccttatgact atggacgttt ctttgaaaaa
     3661 gatgccactc acccagaagc tcgaaaaggt gaaaaatggt ctgattttga cctacgtacc
     3721 atggtcgaaa gaggcaaaaa caaccctgct atcttcatgt ggtcaattgg taatgaaata
     3781 ggtgaagcta atggtgatgc ccactcttta gcaactgtta aacgtttggt caaggttatc
     3841 aaggatgttg ataagactcg ctatgttacc atgggagcag ataaattccg tttcggtaat
     3901 ggtagcggag ggcatgagaa aattgctgat gaactcgatg ctgttggatt taactattct
     3961 gaagataatt acaaagccct tagagctaag catccaaaat ggttgattta cggttcagaa
     4021 acatcatcag caacccgtac acgaggaagt tactatcgcc ctgaacgtga attgaaacat
     4081 agcaatggac ctgagcgtaa ttatgaacag tctgactatg ggaatgatcg tgtgggttgg
     4141 gggaaaacag caaccgcttc atggactttt gaccgtgaca acgctggcta tgctggacag
     4201 tttatctgga caggtacgga ctatattggt gaacctacac catggcacaa ccaaaatcaa
     4261 actcctgtta agagctctta ctttggtatc gtagatacag ccggcattcc aaaacatgac
     4321 ttctatctct accaaagcca atgggtttct gttaagaaga aaccgatggt acaccttctt
     4381 cctcactgga actgggaaaa caaagaatta gcatccaaag tagctgactc agaaggtaag
     4441 attccagttc gtgcttattc gaatgcttct agtgtagaat tattcttgaa tggaaaatct
     4501 cttggtctta agactttcaa taaaaaacaa accagcgatg ggcggactta ccaagaaggt
     4561 gcaaatgcta atgaacttta tcttgaatgg aaagttgcct atcaaccggg taccttggaa
     4621 gcaattgctc gtgatgaatc tggcaaggaa attgctcgag ataagattac gactgctggt
     4681 aagccagcgg cagttcgtct tattaaggaa gaccatgcga ttgcagcaga tggaaaagac
     4741 ttgacttaca tctactatga aattgttgac agccagggga atgtggttcc aactgctaat
     4801 aatctggttc gcttccaatt gcatggccaa ggtcaactgg tcggtgtaga taacggagaa
     4861 caagccagcc gtgaacgcta taaggcgcaa gcagatggtt cttggattcg taaagcattt
     4921 aatggtaaag gtgttgccat tgtcaaatca actgaacaag cagggaaatt caccctgact
     4981 gcccactctg atctcttgaa atcgaaccaa gtcactgtct ttactggtaa aaaagaagga
     5041 caagagaaga ctgttttggg gacagaagta gcaagagttc gtacattaat tggtaaagaa
     5101 ccaaaaatgc cgaaaacagt aggttttgta tacagcgatg gtagtcgtga aaaacttcca
     5161 gtaacttggt ctcaagtcga tgttagtcaa gcaggagttg tgactgttaa aggtacagca
     5221 aatggacgtg aggtagaggc tcatgtcgag gttctagcaa ttgcgaaaga attaccaaca
     5281 gttaaacgta ttgctccaaa tactgacttg aattctgtag acaaatctgt ttcttatgtt
     5341 ttgactgatg gaagtgtaca agagtacgaa gtagatagct gggagattac ggaagtagat
     5401 aaagccaaac tttcagtagc tggatcacgt attcaaatga ctggtcagtt agctggagaa
     5461 actattcatg caacccttgt ggtagaagaa ggaaatgctg cagcacctgt agtgccaact
     5521 gttactgttg gaggtgaagc agtaacaggt cttactagtc gacaaccaat gcaatatcgt
     5581 actctatctt atggtgccca attgccagaa gtcacagcaa gtgctgaaaa tgctgatgtg
     5641 acagttcttc aagcaagcgc agcaaacggc atgcgtgcaa gcatatttgt tcagcctaaa
     5701 gatggtggcc ctcttcaaac ctatgcaatt caattcctag aagaagcacc aaaaattgct
     5761 cacttgagct tgcaagtgga aaaagctgac agtctcaaag aagaccaaac tgtcaaattg
     5821 tcggttcgag ctcactatca agatggaacg caagctgtat taccagctga taaagtaacc
     5881 ttctctacaa gtggtgaagg ggaagtcgca attcgtaaag gaatgcttga gttgcataag
     5941 ccaggagcag tcactctgaa cgctgaatat gagggagcta aaggccaagt tgaactcact
     6001 atccaagcca atactgagaa gaagattgcg caatccatcc gtcctgtaaa tgtagtgaca
     6061 gatttgcatc aggaaccaag tcttccagca acagtaacag ttgagtatga caaaggtttc
     6121 cctaaaactc ataaagtcac ttggcaagct attccgaaag aaaaactaga ctcctatcaa
     6181 acatttgaag tactaggtaa agttgaagga attgaccttg aagcgcgtgc aaaagtctct
     6241 gtagaaggta tcgtttcagt tgaagaagtc agtgtgacaa ctccaatcgc agaagcacca
     6301 caattaccag aaagcgttcg gacatatgat tcaaatggtc acgtttcatc agctaaggtt
     6361 gcatgggatg cgattcgtcc agagcaatac gctaaggaag gtgtctttac agttaatggt
     6421 cgcttagaag gtacgcaatt aacaactaaa cttcatgttc gcgtatctgc tcaaactgag
     6481 caaggtgcaa acatttctga ccaatggacc ggttcagaat tgccacttgc ctttgcttca
     6541 gattcaaatc caagcgaccc agtttcaaat gttaatgaca agctcatttc ctacaataac
     6601 caaccagcca atcgttggac aaactggaat cgtagtaatc cagaagcttc agtcggtgtt
     6661 ctgtttggag attcaggtat cttgagcaaa cgctccgttg ataatctaag tgtcggattc
     6721 cacgaagacc atggagttgg tgcaccgaag tcttatgtga ttgagtatta tgttggtaag
     6781 actgtcccaa cagctcctaa aaaccctagt tttgttggta atgaggacca tgtctttaat
     6841 gattctgcca actggaaacc agttactaat ctaaaagccc ctgctcaact caaggctgga
     6901 gaaatgaacc actttagctt tgataaagtt gaaacctatg ctattcgtat tcgcatggtt
     6961 aaagcagata acaagcgtgg aacgtctatc acagaggtac aaatctttgc gaaacaagtt
     7021 gcggcagcca aacaaggaca aacaagaatc caagttgacg gcaaagactt agcaaacttc
     7081 aaccctgatt tgacagacta ctaccttgag tctgcagatg gaaaagttcc ggcagtcaca
     7141 gcaagtgtta gcaacaatgg tctcgctacc gtcgttccaa gcgttcgtga aggtgagcca
     7201 gttcgtgtca tcgcgaaagc tgaaaatggc gacatcttag gagaataccg tctgcacttc
     7261 actaaggata agaacttact ttctcataaa ccagttgctg cggttaaaca agctcgcttg
     7321 ctacaagtag gtcaagcact tgaattgccg actaaggttc cagtttactt cacaggtaaa
     7381 gacggctacg aaacaaaaga tttgtcagtt gaatgggaag aagttccagc agaaaatctg
     7441 acaaaagcag gtcaatttac cgttcgaggc catgtccttg gtagtgatct tgttgctgag
     7501 gtcactgtac gagtgacaga caaactaggc gaagctcttt cagataaccc taactatgat
     7561 gaaaacagta accaggcctt tgcttcagca accaatgata ttgacaaaaa ctctcatgac
     7621 cgcgttgact atctcaatga cggagatcat tcagaaaatc gtcgttggac aaactggtct
     7681 ccaacaccat cttctaatcc agaagtatca gcgggtgtga tcttccgtga aaatggtaag
     7741 attgtagaac ggactgttgc gcaagccaaa cttcgtttct ttgcagatag tggtacggat
     7801 gcaccaacta aactcgtttt agaacgctat gtcggtccag agtttgaagt gccaacctac
     7861 tattcaaact accaagccta cgacgcagac catccattca acaatccaga aaattgggaa
     7921 gctgtgcctt atcgtgcgga taaagacatc gcagctggtg atgaaatcaa cgtaacattt
     7981 aaagctgtca aagccaaagc tatgagatgg cgtatggagc gtaaagctga caagagcggt
     8041 gttgcgatga ttgagatgac cttccttgca ccgagtgaat tgcctcaaga aagtacgcaa
     8101 tcgaagattc ttgtagatgg aaaagaactt gctgatttcg ctgaaaatcg tcaagactat
     8161 caaattacct ataaaggtca acggccaaaa gtctcagttg aagaaaacaa tcaagtagct
     8221 tcaactgtgg tagatagtgg agaagatagc cttccagtac ttgttcgcct cgtttcagaa
     8281 agtggaaaac aagtcaagga ataccgtatc cacttgacta aggaaaaacc agtttctgag
     8341 aagacagttg ctgctgtaca agaagatctt ccaaaactcg aatttgttga aaaagatttg
     8401 gcctacaaga cagttgagaa aaaagattca acactgtatc taggtgaaac tcgtgtagaa
     8461 caagaaggaa aaactggtaa agaacgtatc tttacagcga ttaatcctga tggaagtaag
     8521 gaagaaaaac tccgtgaagt ggtagaagct ccgacagacc gcatcgtctt ggttggaacc
     8581 aaaccagtag ctcaagaagc taaaaaacca caagtgtcag aaaaagcaga tacaaaacca
     8641 attgattcaa gtgaagctag tcaaactaat aaagcccagt taccaaatac aggtagtgcg
     8701 gcaagccaag cagcagtagc agcaggttta gctcttctag gtttgagtgc aggattagta
     8761 gttactaaag gtaaaaaaga agactagaaa ttttgatacc ttctttatca ttaaagacca
     8821 aagaaaaact cttgtcctaa atagaagata aagagtctgg aacaaaatag ttctcaacca
     8881 caaaaaagct agatatttcc aattgcggaa ctctagcttt ttaattttga gtcgttctct
     8941 tattgtatag tagattgaaa ctagagtagt acacttctac ttctaaaaca ttgttagaaa
     9001 tcgatttgac tgtccttatc gatttgtcct gttcttattt catttaacta tattttagga
     9061 ggttattggc cataagtacc aatcccatgt caattctcac ttgacgcttg cccctcagat
     9121 tacatctctt gtaacccaaa caagccttta actgcccaaa gacaggttcc acatcaatct
     9181 tgcgttgagc gaaaatctgt ctaccttggg gagataaaag cgcctgacat tctttagctt
     9241 tcaagttttg atagcgttcg ttcatatata gccccctttt gaggggctga ttcaggttca
     9301 tcagcgtagt aaaccttgat ttcctgttga aagtctgtct gtgttttctg atgtttggta
     9361 tggtgaaaac gataatacca gccatcagga tgtgtgtagc tatcctcctt gtcattatag
     9421 tgccaattcg ctaagttttt agctgactgt ttataccctc tcttttgttc cttatcaaac
     9481 atggcatatt taatcagaag gggagtgatt tcttcttgaa aatagacttg tatctgttct
     9541 tgaagtttgg cggagaattt ctctgtcgtt ttcttccaca caaaactata cttgttggca
     9601 ttggcttcaa tcttagttcc gtcaataaac aaacaatcta aggtcactaa ctcttccatt
     9661 tttcaatttt tcgaccagag aaaatccctt gtgaataggc aaatagaaga gtagatacaa
     9721 gcattttaag gtgataagac gggcgatcaa aggcatgata gaaggcgtgg aaatgacaat
     9781 cctccaaggt attcaccact ttttcaatag taaaaacgag atggtcttgt ggcaagaagg
     9841 aactgatttt tagtggtaaa gttgtttgat ttgtgttata gtgaatatgc atgggatagc
     9901 ctttctaaat ggtttattgt gcaattctat tttaccaaga ctatcgcaac catgcaaaaa
     9961 agcaacggca aatccgttgc tttttttgga gataagagac tattgtccca gactcttttc
    10021 tttgtataaa attgtatagt gttattgtct atactatttt gtagagaaag caaaaatccc
    10081 tagaaaatgc ttagggattc gactatttca ttaagaacaa ttcaacaatc atttctacat
    10141 acatgataag ggttaataag aatcctgcac gccagaagaa tttgatgaat ttaggatagt
    10201 aaaagctgcg ttttttcaaa aggaagaaaa agacgagaat aatggctagg atcgagaggg
    10261 ttagccccag tctagggagg aaattatggg taaaggtttt agctgtaatc aggtaatatt
    10321 caaataccaa gaccggaaaa gccaaatccg caaagtttcg tcctagtttt tttaatctaa
    10381 aaagtttggt taggataatg cagacgacta aggtcagtat caataataaa atagatgcta
    10441 atttcattaa aatcataccc atattgtatc ataaaaaaag gctaatggaa acgagaaaca
    10501 gaggaattta taggaaagtc aggcttttga gattgtgggt gatttttgtt ataatgaaag
    10561 ttataaaatc ttataatacc ttgaatgatt attatcgaaa actctttgga gaaaagactt
    10621 ttaaagtccc tattgatgcg ggatttgact gtcccaatcg tgatggaact gtggctcatg
    10681 gaggctgtac tttttgtacg gtttcaggtt ctggagatac tattgtagcc ccagatgcgc
    10741 ctatccgtga gcaattttat aaggaaattg actttatgca ccgtaagtgg ccggatgttc
    10801 agaagtatct ggtttatttt caaaatttta ccaacaccca tgaaaaggtg gaagttattc
    10861 gagagcgtta tgaacaggct atcaatgagc caggtgtggt aggaatcaat attggaatgc
    10921 gcccagactg tttaccagac gaaaccatcg aatatttggc tgagttatcg gagtgcatgc
    10981 atgtgacggt tgaattgggc ttgtagacta cttatgaagc aacttctgac ctgattaacc
    11041 gtgcgtattc ttatgaattg taggtggaaa cggtcaagcg tttgagaaag tatcccaaga
    11101 ttgagattgt ttcccatttg attaatggtt tgcctggtga aacccatgag atgatggttg
    11161 aaaatgtccg ccgttgtgtc acgaataacg atattcaagg gattaaactg catttgctcc
    11221 atcttatgac aaatactcgt atgcaacgag attaccatga gggacgttta caattgatga
    11281 gtcaggacga atatgtcagg gtcatctgtg accaactgga aattattccc aaacatatcg
    11341 tcatccatcg aatcacagga gatgcaccta gggatatgct gattgggctt atgtggagcc
    11401 tcaataaatg ggaagctctc aacagcattg agatggagat gcgacgtcgt ggaagtgttc
    11461 aaggatgcaa ggctgtaaaa caggagtttg aaaatgaaaa gactacttga gatggcacat
    11521 gattttttgg ctgaggtcgt gacaaaagag gatgtcgtag tggatgcgac tatgggaaat
    11581 ggtcatgaca cgcttttttt agccaagcta gccaagcaag tctatgcctt tgatattcag
    11641 aagcaagcct tgaaaaagac ccaagagcgt ttgcatcagg ctggcttgac aaatgcccag
    11701 ttaatcttgc aaggccatga gacactggac cagtttgtga caaaaactaa ggcagggatt
    11761 tttaatctgg gctatttgcc gtcagctgat aagtctgtca tcacccgacc gcagacaacc
    11821 attgaagcat tagaaaagct atgtcatttg cttgtcaaag gtggacgaat tgctattatg
    11881 atttactatg gtcatgaagg aggcgacctc gagagagatg ctgtcttgga ttttgtgatc
    11941 cagttgaacc aacaagagta cacagctgcc atttaccgaa ctttaaacca agtcaacaac
    12001 ccgccgtttt tagtgatgat tgaaaaatta gagagataca gacatggata aacaatacct
    12061 acacgaaaag ctggatgcca tgcgccagaa ttttgttgaa tcaacccacc acgaacgagc
    12121 ggtgggcgtg ctagaccaag cgcatatgag caaaaaaatg cttaaaatca agaaaaaatt
    12181 agttgctctt gaaatggaac ggtgccagag aaaaattgag cacaaagact gttccaagat
    12241 tgaccaaaaa atcaaagagc agaaggagat atttgaatcc tgttgtaaaa aagattaagg
    12301 aggacgtgcg tggaattact gatttacctc atcctatttt tactggtttt gattgtctca
    12361 agtacaacca ataagctcct gccctttttg cctctccctt tggtgcagat tcttttggga
    12421 attgtgattg gtctcttttt acccaatact gactttcacc ttaatacgga gttgtttttg
    12481 gcactggtta tcggaccctt gcttttccga gaggctgaag aagcagatgt tacggctatt
    12541 ttaaaacact ggcgaatcat tgtttatctc atatttccag tgatttttat ctcgaccctg
    12601 agtttgggtg gcttggccca tcttctttgg ttcagccttc ccttggcagc ttgcttggct
    12661 gttggtgcag cccttggtcc tacggacttg gtggcctttg cctctctttc ggagcgtttt
    12721 agctttccta agcgcgtgtc caatattctt aagggcgaag gactcttgaa tgatgcttct
    12781 ggtttggtgg cttttcaggt agctttgaca gcttggacaa ctggagcttt ttctctgggg
    12841 caagctagca gttcgctcat cttttcaatc ctaggcggtt ttttaattgg atttttaaca
    12901 gccatgacca accgcttcct ccataccttc ttgctaagtg tgcgcgcaac ggatattgcc
    12961 agtgaacttt tattagaatt gagtttgcct ctagtgacct tctttctggc agaagaagtc
    13021 catgtttcag ggattattgc cgtcgtagtt gctggaattt taaaggcaag tcgcttcaag
    13081 aaaatcacgc tcctcgaagc ccaagtggat acggtgaccg agacggtctg gcatacagtg
    13141 acctttatgc tcaacggttc tgtctttgtg attttaggga tggagttgga aatgatagca
    13201 gaacctatct tgaccaatcc aatctataat cctctacttt tattgctatc tctcatcgcc
    13261 cttacctttg tcctctttgt cattcgtttt attatgatct atggctatta tgcctataga
    13321 acccgacgcc tcaagaaaaa gctaaataag tatatgaagg acatgtttct cttgaccttt
    13381 tcaggtgtta agggaacggt gtcgattgct acgattctct tgataccaag taatctagaa
    13441 caggagtatc ctctcttgct tttccttgtt gcaggtgtga cgcttgtcag ctttttaaca
    13501 ggtctcttgg tcttgcctca tctttctgat gaagaggaag aaagcaagga ttatctcatg
    13561 catatcgcca ttttgaatga agtaacgcta gagttggaaa aagagttgga agacaccaga
    13621 aataaacttc ccctctatgc ggctattgac aattatcatg gacgtattga aaatctcatt
    13681 ttaagccaag aaaaccagga tgatcaagaa gattgggctg ctttgaaact cttgattctt
    13741 agtattgaaa gtgatggttt ggagcaggcc tatgaagagg ggaacattag caatcgtgct
    13801 taccgagttt accaacgtta tctgaaaaat atagaacaag gaatcaatcg taaacttgcc
    13861 tcaagactga cctattattt ccttgtttct ttgcggattt tacgttttct ccttcatgaa
    13921 gtttttactc tcggaaagac cttccgtagc tggaagaaca aggagcaaag ccgtctccgt
    13981 gctcttgatt atgaccaaat tgcagagctc tatcttgcca atacagagat gattattgaa
    14041 agtttggaaa acctgaaggg agtctacaga cgctctttga ttagttttat gcaggagtct
    14101 cgtcttcgag aaacagctat tatcagcagt ggtgcctttg tcgaacgggt tatcaatcgt
    14161 gtcaaaccca acaatatcga tgaaatgctg agaggctatt atctggagcg caagttgatt
    14221 ttcgaatacg aagaaaaacg attgattacg actaagtatg ccaagaaatt acgacaaaat
    14281 gtaaataact tagagaacta ttccttgaag gaagctgcca ataccctacc gtatgatatg
    14341 gtggaattgg taagaagaaa ttagttaata ctcttcgaaa atctcttcaa accacgtcag
    14401 cgtcgcctta ccgtatgtat gtgactgact tcgtcagtct tatctacaac ctcaaaacag
    14461 tgttttgagc agcttgcggc tagcttccta gtttgctctt tgattttcat tgagtataag
    14521 attataagtg aaggagtgtg acatgaaaaa gtggtggaaa gagctgatag acaagccttt
    14581 attaaaagct tttttgcatt attatcaagc atcagatagt gagttgacca gtgtcgcagt
    14641 agcctactat tggttgattt cgattttccc cctgcttttg gtggtggtca atatcctccc
    14701 ctattttcag attcctgtgg gagaatttct gggctttatg aaagacgtcc tgcccccaag
    14761 cctctatgaa ggtgtggaaa aaatagccag agaagtcttg acgcaacctt caacaggttt
    14821 attgagtttt tctgttttat cggcgttatg gagtttttca aaatctatga atttcttgca
    14881 aaaagctttt aacaaggctt atggcgtcga aaagagtcgt ggacttattt cccatcagat
    14941 gctaagtctc ctagtcagtt ttggcttgca gctcctcttt gcgtttgcct tgtttttgat
    15001 cttatttggt cagatgattt tggctttact tgctcattat tggacaaaag atggcattat
    15061 ctatcaggct ttacaagggt tggcaggtcc tctgatttac gccctcctct ttgctatctt
    15121 ggtcatgctt tattattttc ttcccaatat atctaacagg aaaattagct ataccctacc
    15181 aggcagtgcc tttgttctct tagtgatttt aggtttacta actctgttct ctagttatct
    15241 caattactat gtccatcatt tggtggatgt ccgaatttta ggttccgttc ttcttgttgt
    15301 tatgatgttt tggtttatct tgattgctaa aattgttatc ctaggcgcgg ttatcaatgc
    15361 cagtatgcag agtttgaaag atccggtctt taaaaaagac taactattta tccattacaa
    15421 aagcgcatag tatcaaaact ttatagaacc ttgatactat gcgcttttgt gctttgacag
    15481 ttttttgtat agtttttgtc caagatttct aaatcatctg attaaattcc tattttcccc
    15541 tatcattgtc cctgtttttt cgaaaatgga aggagtataa taaatctatc aataaaatga
    15601 taggaggaac tttatgggtc atattttctt ttttctaagt gtctttttgg cagggattct
    15661 atccttcttt tctccttgta tcttaccttt gttaccggtc tatacaggag tgttactaga
    15721 tgataaggat ggtgctcagg cttctagcgg caaattttca atctcagtta ctagtttatt
    15781 acgaactctg gcctttatag caggaatttc ctttatattt attttgttgg gctatggagc
    15841 tggtttttta ggcgatttgc tttatgcttc ttggttccaa tatcttactg gggcaattat
    15901 tatccttctt ggtttgcacc aaatggagat tctacacttt aaggggcttt ataaggaaaa
    15961 gaggctacaa ctgcaaggac aggggcaaaa tggtaagggc tatagtcagg catttttatt
    16021 gggcttgacc tttagttttg cttggacgcc ttgcgtgggg ccggttctgg ggtctgtttt
    16081 ggccttggcg gcttcaggtg gttcaggagc ttggcaggga gctggtctca tgttggtgta
    16141 tacgctgggc ttggcgctac cattcttgct tctagctctg acctctagtt atgttttgaa
    16201 acatttccga aaacttcatc cctatctcgg aatcctcaaa aaagtgggtg gttttctcat
    16261 tattgtgatg ggactcttgg ttctgtttgg aaatgcttca attttaagtc aattatttga
    16321 ataaaatgga aaggaatatc aatatgaaaa aatggcaaac atgtgttctt ggagcaggtt
    16381 cgctcctttg tttgacggct tgttcaggca agtccgtgac tagtgaacac caaacgaaag
    16441 atgaaatgaa gacggagcag acagctagta aaacaagcgc agctaaaggg aaagaggtgg
    16501 ctgattttga attgatggga gtagatggca agacctaccg tttatctgat tacaagggca
    16561 agaaagtcta tctcaaattc tgggcttctt ggtgttccat ctgtctggct agtcttccag
    16621 atacggatga gattgctaaa gaagctggtg atgactatgt ggtcttgaca gtagtgtcac
    16681 caggacataa gggagagcaa tctgaagcgg actttaagaa ttggtataag ggattggatt
    16741 ataaaaatct cccagtccta gttgacccat caggcaaact tttggaaact tatggtgtcc
    16801 gttcttaccc aacccaagcc tttatagaca aagaaggcaa gctggtcaaa acacatccag
    16861 gattcatgga aaaagatgca attttgcaaa ctttgaagga attagcctag gaggtgtctt
    16921 atgaatgata agttaaaaat cttcttgttg ctaggagtat tttttctagc cataaccggt
    16981 ttctatgttc tattgatacg aaatgcaggg cagacagatg cctcgcaaat tgaaaaggcg
    17041 gcagttagcc aaggaggaaa agcagtgaaa aaaacagaaa ttagtaaaga cgcagacttg
    17101 cacgaaattt atctagctgg aggttgtttc tggggagtgg aggaatattt ctcacgcgtt
    17161 cccggggtga cggatgccgt ttcaggctat gcaaatggta gaggagaaac aaccaagtac
    17221 gaattgatta accaaacagg tcatgcagaa accgtccatg tcacctatga tgccaagcaa
    17281 atttctctca aggaaatcct gcttcactat ttccgcatta tcaatccaac cagcaaaaat
    17341 aaacaaggaa atgatgtggg gacccagtac cgtactggtg tttattacac agatgacaag
    17401 gatttggaag tgattaacca agtctttgat gaggtggcta agaaatacga tcaacctcta
    17461 gcagttgaaa aggaaaactt gaagaatttt gtggtggctg aggattacca tcaagactat
    17521 ctcaagaaaa atccaaatgg ctactgccat atcaatgtta atcaggcggc ctatcctgtc
    17581 attgatgcca gcaaatatcc aaaaccaagt gatgaggaat tgaaaaagac cctgtcacct
    17641 gaggagtatg cagttaccca ggaaaatcaa acagaacgag ctttctcaaa ccgttactgg
    17701 gataaatttg aatccggtat ctatgtggat atagcaactg gggaacctct cttttcatca
    17761 aaagacaaat ttgagtctgg ttgtggctgg cctagtttta cccaacccat cagtccagat
    17821 gttgtcacct acaaggaaga taagtcctac aatatgacgc gtatggaagt gcggagccga
    17881 gtaggagatt ctcaccttgg gcatgtcttt acggatggtc cacaggacaa gggcggctta
    17941 cgttactgta tcaatagcct ctctatccgc tttattccca aagaccaaat ggaagaaaaa
    18001 ggctacgctt atttactaga ttatgttgat taatactctt cgaaaatctc ttcaaaccac
    18061 gtcagcgtcg ccttaccgta tgtagggtta ctgacttcgt cagttttatc tacaacctca
    18121 aagctatact ttgagctgac ttcgtcagtt ttatttacaa cctcaaagcg gtactttgag
    18181 aaacctgtgg ctatcttcct agtttacttt ttgattttta ttgagtataa gaggtctttc
    18241 ctaagcagtt agaggagaat tttgctatac tgatactagt aagtggtaaa ggagcagatc
    18301 atgacctaca caatcttaat cgtagaagat gaatatctgg taagacaagg tttgactaaa
    18361 ctggtcaatg tagcagccta cgatatggaa atcatcggtc aggctgaaaa tggaaggcag
    18421 gcttgggaat tgatccaaaa gcaggtgcca gatatcattt taaccgatat caacatgcct
    18481 catctaaatg gcatccagtt ggccagtctg gtacgagaaa cctatcctca ggttcatttg
    18541 gtctttttaa caggttacga tgattttgat tatgccttgt ctgctgtcaa actaggtgtg
    18601 gacgactacc tgctcaaacc cttttctcgt caggatattg aggaaatgtt ggggaaaatc
    18661 aaacaaaaac tagacaagga agagaaagaa gagcagttac aagatttatt gactaatagg
    18721 tttgaaggaa acatggccca gaaaatccag tctcatctgg ctgatagcca atttagttta
    18781 aagtctttag ccagtgactt aggttttagt ccgacctatc tgagttcctt gattaagaaa
    18841 gagttgggct tgccttttca ggattatctg gtgagagaac gtgttaaaca agccaagctc
    18901 ttgcttttaa ctacagatct gaagatttat gagatcgcag agaaggttgg ttttgaagat
    18961 atgaactatt ttacccaacg ttttaagcag attgcaggtg tgacacctcg tcagtttaag
    19021 aagggagaag accgatgaag cgttcttctc ttttagttag aatggttatt tccatctttc
    19081 tggtctttct cattctccta gctctggttg gaactttcta ctatcaatca agttcttcag
    19141 ccattgaggc caccattgag ggcaacagcc aaacgaccat cagccagact agccacttta
    19201 ttcagtctta tatcaaaaaa ctagaaacca cctcgactgg tttgacccag cagacggatg
    19261 ttctggccta tgctgagaat cccagtcaag acaaggtcga gggaatccga gatttgtttt
    19321 tgaccatctt gaagtcagat aaggacttga aaactgttgt gctggtgacc aaatctggtc
    19381 aggtcatttc tacagatgac agtgtgcaga tgaaaacttc ctctgatatg atggctgagg
    19441 attggtacca aaaggccatt catcagggag ctatgcctgt tttgactcca gctcgtaaat
    19501 cagatagtca gtgggtcatt tctgtcactc aagaacttgt tgatgcaaag ggagccaatc
    19561 ttggtgtgct tcgtttggat atttcttatg aaactctgga agcctatctc aatcaactcc
    19621 agttggggca gcagggcttt gccttcatta tcaatgaaaa ccatgaattt gtctaccatc
    19681 ctcaacacac agtttatagt tcgtctagca aaatggaggc tatgaaaccc tacatcgaga
    19741 caggtcaggg ttatactcct ggtcacaaat cctacgtcag tcaagagaag attgcaggaa
    19801 ctgattggac ggtgcttggc gtgtcatcat tggaaaagtt agaccaggtt cggagtcagc
    19861 tcttgtggac cttgcttggg gccagtgtca catctcttct tgtctgtctc tgcttagtgt
    19921 ggttcagtct taaacgctgg attgctcctt tgaaggattt gagagaaacc atgttggaaa
    19981 ttgcttctgg tgctcaaaat cttcgtgcca aggaagttgg tgcctatgaa ctgagagaag
    20041 taactcgcca atttaatgct atgttggatc agattgatca gttgatggta gctattcgta
    20101 gccaggaaga aacgacccgt cagtaccaac ttcaagccct ttcgagccag attaatccac
    20161 atttcctcta taacactttg gacaccatca tctggatggc tgaatttcat gatagtcagc
    20221 gagtggtgca ggtgaccaag tccttggcaa cctatttccg cttggcgctc aatcaaggca
    20281 aggacttgat ttgtctctct gacgaaatca atcatgtccg ccagtatctc tttatccaga
    20341 aacaacgcta tggagataag ctggaatatg aaattaatga aaatgttgcc tttgataatt
    20401 tagtcttacc caagctggtc ctacaacccc ttgtagaaaa tgctctttac catggcatta
    20461 aggaaaagga aggtcagggc catattaaac tttctgtcca gaaacaggat tcgggattgg
    20521 tcatccgtat tgaggatgat ggcgttggct tccaagatgc tggtgatagt agtcaaagtc
    20581 aactcaaacg tgggggagtt ggtcttcaaa atgtcgatca acggctcaaa cttcattttg
    20641 gagccaatta ccagatgaag attgattcta gaccccaaaa agggacgaaa gttgaaatat
    20701 atataaatag aatagaaact agctaactcc cagtcaattc tgggagtttt atgcgtaatt
    20761 gggcaaattt tctaggttgt ctatcttgct aaaaacaaga aaaaacggta tgatagaaga
    20821 gtgacaacag aggtaacaga gatgaacgag acacagattc aaagagaaac acgtcaggta
    20881 gtggaagatg ttttagaaaa gaccaatttg aagcaggggg ccctttttgt tttaggtcta
    20941 tcttctagtg aggtgctagg tggtcagatt gggaaagaat ccagtcaaga gattggtgag
    21001 ctgatagtgg agactatctt gggtattcta ggtagtagag gtatccattt agcagttcaa
    21061 ggttgtgagc atgtcaatcg ggctctcgtt gttgaacgtc aggtggcaga gcagtttgat
    21121 ctggaaattg tcagtgtcca tcctactctt catgcaggag gttcggggca gttggcagcc
    21181 tttaagttta tgcaggatcc agttgaggtt gaatttatca aggctcatgc tggattggat
    21241 atcggagaca ctgcaattgg catgcatgtc aagcatgttc aggttccgat tcgccctatt
    21301 ttgagagaga ttggtcatgc ccatgtaacg gcactggcta gtcgtccaaa attaatcgga
    21361 ggtgcgcgtg cgcactatcc gcaagatgct attaggaagt cttgagaatg aataaaggag
    21421 ataaaagtgt ttaaaaaaga ccgtttttca attcgtaaga ttaagggagt tgtaggctct
    21481 gtatttcttg gaagcctttt gatggctcct tctgtagtgg acgcagccac ctatcactat
    21541 gtaaataaag agattatttc acaagaagct aaagatttaa ttcagacagg aaagcctgac
    21601 aggaatgaag ttgtatatgg tttggtgtat caaaaagatc agttgcctca aacagggaca
    21661 gaagcatctg ttttgacagc ttttggtttg ctgactgttg ggagcttgct tttaatctac
    21721 aagagaaaga aaattgctag cgtctttcta gttggagcta tgggattggt agttcttcct
    21781 agtgcagggg ctgtagaccc agttgcgacc ctagcgctgg ctagtcgaga gggtgttgtt
    21841 gaaatggatg gctatcgcta tgttggttat ctatcaggtg acatcctcaa aacgcttggc
    21901 ttggacactg ttttagaaga aacctcagca aaacctggag aggtgactgt agtcgaagtt
    21961 gagactcctc aatcaataac aaatcaggag caagctagga cagaaaacca agtagtagag
    22021 acagaggaag ctccaaaaga agaagcacct aaaacagaag aaagttcaaa ggaagaacca
    22081 aaatcggagg taaaacctac tgacgacacc cttcctaaag tagaagaggg gaaagaagat
    22141 tcagcagaac cagctccagt tgaagaagta ggtggagaag ttgagtcaaa accagaggaa
    22201 aaagtagcag ttaagccaga aagtcaacca tcagacaaac cagctgagga atcaaaagtt
    22261 gaacaagcag gtgaaccagt cgcgccaaga gaagacgaaa aggcaccagt cgagccagaa
    22321 aagcaaccag aagctcctga agaagagaag gctgtagagg aaacaccgaa acaagaagag
    22381 tcaactccag ataccaaggc tgaagaaact gtagaaccaa aagaggagac taaaacagct
    22441 aaggggacgc aagaagaagg caaagaaggt caagcaccag tacaagaagt aaacccagaa
    22501 tataaagtaa caacaggtac agttgagaag tctaccgaaa gtgaattaga tttcacaaca
    22561 gaagtagttc cggatgacac aaaatatgta gatgaagaag ttgttgaacg tcaaggatct
    22621 aaaggagttc aagttacgaa aacaacttac gaaacagtgg aagttgtaga gacagacaaa
    22681 gtcctttcta caactacaga agtgaaaact cctgttgttc ctaaagtcgt taaaaaagga
    22741 acaaaaccag ttgaaggaac agcagttgaa acaagagaag aagtcattcc ttttgctaca
    22801 aaagaacaag aagatgacac tttaaaacgc ggaacaagac aagtagctca agaaggtgta
    22861 aacggtaaga aacaaattac tgaaacctat aaaacaattc gcggagaaaa aacaaacgaa
    22921 gctccaacag tagaagaaac agttctccaa gcacctcaag atgagattat caaaaaaggc
    22981 acaaaaggtt tagaaaaacc aaccttagaa tgggttaaaa cagataaaga tgtcttgaag
    23041 aagagtgcga cagccagtta tacattgaat aaaccagatg gggtagaaat caaatccatc
    23101 aaagtagctc taaaagataa tactggaaca gttattaaag aagtaacggt agaagaaaat
    23161 aacttaaacg ctactttaga caacttgaaa tattatcaag gttatacact atccacaacc
    23221 atggtctata atcgaggcga aggcgaagaa actgaaatgt tagaagataa agaagttcag
    23281 ttagacctta aaaaagttga aatcaaaaac atcaaagaaa ctagcttgat gagtgtggat
    23341 gatgctggtg ttgaaacaga taagagctta cttactgaaa aaccgacaga cgttgctcca
    23401 ctttacctac gagtgacaac tcatgataac aaaacaacaa gactggcagt aagttcagtt
    23461 gaagaagttg ttgttgatgg aaaaacattg tacaaagtgg tagctaaagc tcctgactta
    23521 gttcaacgta gagaagatga tacactaagc gaagagtatg tacattactt tgaaaaacaa
    23581 ttaccaaaag tagataatgt gtactatagc tttaacgagt taattacaga tatgcaaaaa
    23641 aatcctactg gtacattcaa actaggtgca gatcttaatg cagcgaatac tccaactcca
    23701 agtaaatcat atgtaacagg tgaatttaaa ggtaaattat caagtgttga tggtcaacat
    23761 tatacgattc acaatactgc gcgcccatta tttaacaata ttgttggtgg taccgttaaa
    23821 aatattaatc ttaacaatgt aaatatcgat atgccttggg ctgatcaaat tgcatctgta
    23881 gcaaatgtga tcaaaggtgg tagccttatt gagaatgtta aagtcaaagg taatgtgctc
    23941 ggcagaaact gggtatctgg tttcattgat aaaattgaca gtggtggtag attagttaat
    24001 gttgcattta ttggtaatgt aacttctgtt ggagatgggg ataagttctt aacaggtatt
    24061 gttggagaaa actggaaagg atatgtcgaa aaagcatacg ttgaagctaa tattaaaggt
    24121 aagcgagcta aagctgctgg tatatcttat tggtcgcaaa atagcggaga taataatgct
    24181 gtcggtagag atggagcaat taagaaatct gtcgttaaag gttcaataga tgtagaaaaa
    24241 ccaattgaag ttggtggcgc tgttggaagt ttaaattatc ttggttatat agaagacact
    24301 gttgctatga tgaaagttaa gaacggtgaa attttctacg gttcgcatga tatcgatacc
    24361 gatccatatt acacaggaga acgagtaaat agaaatttca ttgttgacgg tgtaagtgaa
    24421 ggtaaatctt cgtatacata ctctaaacaa caaaagcgta ttaaatcaat atctcaagag
    24481 gaagcagata agaaaattaa agaattgggt atcacagcag ataaatttac aattatcgat
    24541 ccaattgtaa ataaacttaa caacatcaaa caaaaagctg atacttataa agatactcaa
    24601 gattacaaag tagaccgtga acttgcttat cgtaatatcg aaaaacttca accattctac
    24661 aataaagagt ggattgtaga ccaaggtaat aaagttccat caaattctaa attattaact
    24721 acagaagtat tatctgttac aggtatgaaa gatggtcaat ttgtgactga cttatcagaa
    24781 attgataaaa ttatgattca ctatgcggat ggtacaaaag aagaaaaatc tgtatcacct
    24841 aagcctacat caaacgtaga acaagtaaaa gaatatggta ttacagattt aggagatgtc
    24901 gtttacacac caaatatggt ggttaaagac cgtgctcaat tactaagtga tgttaaagcg
    24961 aaattagata cgattactct tgaatctcct gaagttcgtg cgataacagg taatgttgga
    25021 gcattatact tagaagaaag ttttgcagaa gtaaaaggaa atcttgataa attagtaaaa
    25081 gctcttgttg aaaatgaaga tcatcagtta aatagtgatg aggcagccat caaagcattt
    25141 cttaagaaag tagaagataa taaagctaaa atcatgatgg cacttactta cctcaatcgt
    25201 tattatggta ttgaatatgg tgacttaaac attaagaata taatgatgtt taaaccagat
    25261 ttctatggca agacaccaag tgttattgat agattaattc gaattggttc gcaagaaaag
    25321 aacttgaagg gtgatagaac ccaagatgca tatcgtgaaa ttattgcagg agatactggt
    25381 aaaggtgatt tacgtagttt cttagattat aatatgcgtc tcttcacaaa tgatacagat
    25441 cttaatgact ggtttatcca ttcagcgaaa aatgtttatg ttgtagaacc agaaacaaca
    25501 aatccagatt tcaaaaataa acgtcatcgt gtttttgatg gtctgaataa taatatgcat
    25561 gcacgtatga ttttaccatt attgaacttg aaaaaagcac atatctttat gatttcaacc
    25621 tataatacta tggcttatag ttcatttgaa aaatatggta aaaatacgga agctgaacgt
    25681 gaagcgttca aggaacgtat caattatgta gctaaagctc aacaaacata cttagacttc
    25741 tggtcacgtc ttgcacttcc aaatgttcgt gatagacttc ttaagagtca aaatatggtt
    25801 cctacacctg tatgggataa tcaagcttat gcgggtatta aggatgctaa tcgtcgtgga
    25861 tatggtacag atggtaaggt agcgacacca attcgtgagt tatttggtcc aacagacaga
    25921 tggcatcaaa ttaattggaa tatgggagct atggctaaag tttatggaaa accatatgaa
    25981 gatgaacaag ttttcttcat ggtaacaaat atgttagagg actttggtat ttcagccttt
    26041 acacatgaaa caactcacat aaatgaccgt atggcatatc ttggtggtca tggacatcgt
    26101 ccaggtacat accttgaagc atatgctcaa ggtatgttac aaacacctga taaatcaaca
    26161 tcaaatggtg agtatggagc attaggtatt aatatggctt atcatcgtca aaatgacgga
    26221 aatcagtggt ataacccaga tcctgataaa ttacaaagtc gtgaacaaat tgatcactat
    26281 atgaagaact acaatgatgc tcttatgatg ttagatcatc ttgaggctga tgctgtatta
    26341 ccgcagttaa atggaaatac gagtcgctgg ttcaagaaaa tggatagaca ataccgtagt
    26401 ggaaattcag gtcatcagtt cgataaaatt cgtgagttga cagaggatga gaaaaaagta
    26461 gcaatcaata caattgatga tttaattact aataacctaa taacgaaaca tggtgctcct
    26521 ggtgatggta cttataatcc aacggacttt aggtctgctt atgttaatat gaacatgatg
    26581 actggtattt atggtggaaa tacaagtaca ggtgcaccgg gtgcagcatc atttaaacat
    26641 aatacattcc gtatgtgggg atactttgga tatgagaatg gatttgttgg atatgcttct
    26701 agcaaatatc aaaatgcagc caataaagaa aataatagga tgttaggtga tgacttcatt
    26761 attaagaaag tttcaaatgg aagattcact aaccttgaag attggaagaa agagtactat
    26821 aaagaagtga aggcaaaagg tgaaaaagga tttactgcaa ttgagattga tggtaaacaa
    26881 attaccaatt atgctgaact aaaagtctta tttgataagg cggttgaggc tgatctagca
    26941 ggtggaggaa cagctaaaac tgtagaactt aaatcgaaag tctttaaagc acttcttaag
    27001 aattcagacg gattcacagg aaatcttttt aaagcataga ttgtattaaa tagatgagta
    27061 aaaaggtaga ttaagttttt cttgatctgc ctttttatgc tatacttaag gtatgcatag
    27121 aaaaacagtg attgatttta gggctttagg ggagagacac atctttactc agcctatcaa
    27181 agagttaaaa accagagatt taacagaagt aacagcctta ttggcacaag tggaaagcta
    27241 ccaagagcaa ggttattatg tggtggggta tgtcagctac gaggctgcac ctgcttttga
    27301 ggaaaaatta gcagttcaca aggatcccct actgggagag tacctgcttt attttactgt
    27361 tcatgatagc gtagaaactt cccctattcc tctgacttat gaggatattg atttgccctc
    27421 aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag gccattgccc aaattcacca
    27481 tcatttacgg cagggagata cctatcaggt caactacacc gtccaactta agcaaaagtt
    27541 aaatgccaat ccttttgcca tctacaatcg tatggtggta gagcaggagg cgggctacaa
    27601 tgcctatgtg gaacatgacg agatggcagt gatttccatg agcccagagc tcttttttga
    27661 gcaaaatgat cgcgagttga caacacgacc aatgaagggg acgactcagc gtggggtaac
    27721 tgaccaagaa gatcttgaac aggccagttg gttggaacag gatcccaaaa atcgctctga
    27781 aaatatgatg attgtggacc tcttgcgcaa tgatatgaac cgtatttctg aagttgggag
    27841 cgagcacgtg gagcgtctgt gtcaggtaga gcagtattca actgtttggc agatgacttc
    27901 gaccatcaag agtcagttgc gagaggatgt ggaccttgtt gaaatcttcc gctcactctt
    27961 tccttgtggt tccataacgg gtgcaccgaa aattgcgaca atggagatta tcaaggactt
    28021 ggagcctcaa ccgcgtggag tctactgtgg aacgattggt ctcttgcttc caaatggacg
    28081 acggattttt aatgtggcca ttcgtaccat tcaacttcac aaaggtcaag ccatttatgg
    28141 agttggcgga gggattactt gggatagcac atgggaatct gaataccgag aggttcatca
    28201 aaaggcagca gttctttatc gtaaacaagc tcgcttccaa ctgattacga caggggaaat
    28261 cagccagaag aacctgctgt ttgaagatca acatctggaa agactgagaa aagccagtcg
    28321 ttattttgcc tttccttttg atgcagaaga cttgggacac aagattgagg aagagtgtca
    28381 ggattgtgaa gctaatcaag attaccgctt gcgaatcagc cttagcaaat ctggagagat
    28441 agaagtcaat cgtcaagtat taacccctct cagtacaagc ttttgtcagg cccaagtctg
    28501 ccttcaggaa gctgctttga atcaatcctt tacctacttt aaaaccactc accgaccgca
    28561 tttgaaccta ggagaacaag agaagattta ccataataag tcaggagaac tgcttgaaac
    28621 ctctatagga aatttggttc tgaaaatcgc tggaaaactc tacacaccgc ctatccgact
    28681 tggaatcttg ccaggaattt accgtcagta tttgctagaa acaggacagg tagaagagaa
    28741 agtcttgacc ttggcagatt tagcccaagc agaagctatt tacggctgta atgcagtgag
    28801 aggcttgtat gaactaagcc ttgaggagaa ctagatgaaa ctgatatttt tgcatgggtt
    28861 agggcagtca gcagagtctt ggaaagaagt tcggaatcta ctgacagatt atccttctga
    28921 ggctatcgag ttatttcctt ctggagttag taactatcaa caagctaagg agcgagttta
    28981 tcagcaccta gctcaagaga cagaaccttt tgttttgatt ggattgtcct taggtgctgc
    29041 cctggcacta gaactttcca gttacgattt accaaatctt cgagctttga ttctgtcagg
    29101 atgcccgctt aaattagcag ggaatatcct attttatctt caattgctga tttttaaact
    29161 acttcccaaa agagtatttg aaaaacaggg agcagacaag actcttatgg tcggagtttc
    29221 tgaggaattg aagacacttg atttaacgga tatagcaggg acttgctcct atccaacctt
    29281 actaatttgt ggcagcaaag ataaaccgaa tcttagttct atgaaagctc tccatagact
    29341 gttaacagat tcgcagtttc agatcattcc tgatggccct catgtcttga ataaggcaaa
    29401 accaaaggag tttgtagaaa agaccagaag tttccttgaa ttgctgaaat aagtttgata
    29461 aaataatatt gaaatcgata tcataattat gggagaaaga aatgaataaa cgtctatttt
    29521 taaaaatgag tctggtgacg ttgccaattt tagccttgtt ttcacaacca gttttggcgg
    29581 aagaaaacat ccatttttcg agctgtaagg aagcttgggc gaatggctat tcggatattc
    29641 acgagggaga acctggttat tctgccaagt tagaccgtga tcatgatggt gtggcttgcg
    29701 aattgaaaaa tgctcctaag ggtgctttta aagcaaaaca gtcaacggct attcaaatca
    29761 acacaagttc agcaacaaca agtggttggg ttaagcagga cggcgcttgg tactactttg
    29821 atggaaatgg aaatctagtg aaaaatgcat ggcagggaag ctattacctg aaagctgatg
    29881 gtaaaatggc acagagtgaa tggatttatg actcttctta tcaagcttgg tattatttga
    29941 aatcagatgg ttcttatgca aaaaatgcat ggcaaggagc ttattacctt aaatcaaacg
    30001 gtaaaatggc acaaggtgag tgggtttatg attcttctta ccaagcatgg tattacttga
    30061 aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa ctactatttg aaatcagatg
    30121 gtaaaatggc taaaggtgaa tgggtttatg ttgccaccta tcaagcttgg tattatttga
    30181 catcagatgg ttcttatgct tacagtacat ggcaaggaaa ttactatcta aaatcggatg
    30241 gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc gctgacggag
    30301 tttggaagga aggtcaagca agtacagctt cttctagtaa tgatagcaat agtgaatatt
    30361 ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca aaaaaaacgt
    30421 atgtatagac aattaacttc tgattttgat aaattttcaa atgatgcagc tcaatatgcc
    30481 attgatcatt agatgattaa cagatactgg taggcgaaaa aattccttat aattcattta
    30541 cgcaaacctt tgcataagtg aatggatttt gatatactag tactgtaagc attttcaatt
    30601 actacacaat gaaaaaggag aatatgatga gtcaaaagat tattgggatt gaccttggtg
    30661 gaacttctat caaatttgca atcttaacaa cagcaggaga aatccaagga aaatggtcaa
    30721 tcaagaccaa cattttggat gagggaagtc atatcgttga tgatatgatt gagtctattc
    30781 aacatcgttt ggacttgctt ggattggcag cagcggactt ccaaggcatt ggaatgggat
    30841 caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg tgcctacaac ttgaactgga
    30901 aaacccttca accaatcaaa caaaagattg aaaaagcttt gggcattcca tttttcatcg
    30961 ataatgatgc caacgtagca gctcttggtg agcgctggat gggtgctgga gataaccaac
    31021 cagacgttgt ctttatgaca ctcggtactg gtgttggtgg tggtattgtc gcagaaggca
    31081 aattgcttca tggtgttgct ggtgcagcag gtgaacttgg tcatatcact gttgactttg
    31141 accaaccaat ctcatgtact tgtggtaaaa aaggctgcct tgagacagtt gcttcagcaa
    31201 cagggattgt caacttgact cgtcgctatg ccgatgaata cgaaggcgat gcagccttga
    31261 aacgcctgat tgataacgga gaagaagtaa ctgctaagac tgtctttgat ctcgcaaaag
    31321 aaggagacga ccttgctttg attgtttacc gtaacttctc acgttacttg ggaatcgctt
    31381 gtgctaacat tggttcaatc ctaaacccat caacaatcgt catcggtggt ggtgtgtcag
    31441 ctgcgggaga attccttcta caaggtgttc aaaaagttta cgatgaaaat agtttcccac
    31501 aagtacgcac atctactaaa ttagctcttg caactctagg aaatgacgct ggagttatcg
    31561 gagcagcatc acttgtattg caataagata atgagagagg aaaatgctaa cctagagtta
    31621 gtaatgttcc tcttttattt tttatgctat actagttagg acaatgaatg aggtgaaacg
    31681 aaatgacaaa agcagatacg atttttaaag agaatattga acgcattcta aaggaaggtg
    31741 ttttctcaga gcaggctcgt cctaagtaca aggatgggac tgttgctaat tccaagtatg
    31801 taactggtgc cttttccgag tacgacttat ctaaaggaga atttcctatc acaaccttgc
    31861 gtcccattgc aatcaaatcc gccatcaaag aagttctctg gatttaccaa gaccagtcaa
    31921 atagcttaga ggtacttaat gataagtaca atgttcacta ctggaatgac tgggaagtgg
    31981 gggatacagg aactattggt gaacgctacg gtgctgttgt taagaaacac gacatcatca
    32041 acaagctttt caaacagttg gaagccaacc cttggaaccg ccgcaatatc atttcgctct
    32101 gggattacca agctttcgaa gaaacagatg ggctactccc atgtgccttt cagaccatgt
    32161 ttgatgtccg ccgtatagat ggagaaatct atctggatgc gaccttgacc cagcgctcga
    32221 atgacatgct agtagcccac cacatcaacg ctatgcagta tgtggctttg cagatgatga
    32281 ttgccaagca ttttggctgg aaggttggaa agttcttcta cttcatcaac aacctccata
    32341 tctatgacaa tcaatttgaa caagctcagg aattactccg tcgtgaaccg tcaaactgcc
    32401 aaccacgttt ggttttgaat gttcctgatg gaactaattt ctttgatatc aaagcagaag
    32461 attttgaatt ggtggattat gaccctgtta agccgcagtt gaagtttgac ctagctattt
    32521 aaaagaatag aaaaaagaag ttgagaataa tcccaacttc ttttgtttct taacgtgata
    32581 cgcggcgacg agctgctttt ttacggtttt cttcgatgaa agctgctttt tgctcttctg
    32641 gttcgattac tttcttttta aatgcgtata ctgcacctgc aacggcagcg acagttcctg
    32701 cgacacctgt tacaagacct ttagcgaatc ctttagccat gagtcttcct cctttatatt
    32761 ctcaatcagc cagcctcctc aagaggtcac atttttctga ctgacctttt tgtgttataa
    32821 taatagtaac gaaaaaatgg gaatttttca aggaaaaaag atgaaaacaa aaataattgt
    32881 gattgttgga ccgactgctg ttggaaagac ggctctagct attgaagttg caaagcgttt
    32941 taatggcgaa gtggttagtg gagatagcca acaagtctat cgaggacttg atattgggac
    33001 ggccaaggct agtccagaag agcaggcagc tgttcctcat catttaatcg atgttagaga
    33061 gataaccgag tcttactcgg cttttgattt tgtttcagaa gctaagatga ctattgagga
    33121 tattcacagc cgtggcaagc tagccattat cgccggtggg actggacttt atatccagag
    33181 cttgctagaa ggttaccacc taggtgggga gactcctcat gaggagattt tagcttatcg
    33241 agctagtttg gagccatatt cagatgagga attagcccat ttggtggagc aagcaggcct
    33301 tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc ttagaaattg cccattttgg
    33361 tcaggatttg gaaaatcaag agattttgta tgaaccgctg attatctgct tggatgatga
    33421 acgtagtcaa ctttatgagc gtatcaacca ccgagtggat ttgatgtttg aggctgggct
    33481 tttggatgag gccaagtggc tgtttgacca ttcccctaat gtacaggctg ctaaaggaat
    33541 tggctataag gaactctttc cttatttccg tggagagcag acctttgagg aagctcgtga
    33601 gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag ctgacctggt tccgtaatcg
    33661 catgcaggtc accttttatc agattggaga atctggtgta caagaccgca ttttaagcca
    33721 gatagaggag tttttagatg attgaaacgg agaaaaaaga ggagcgagtc ctgctgattg
    33781 gtgtggaatt gcagggtatg gacagttttg acctctccat ggaagaattg gctagtttag
    33841 cgaaaacggc aggggcagtc gttgtagata gctacagaca aaaacgtgaa aaatatgatt
    33901 ccaagacctt cgtcggctct ggtaagttgg aagagattgc gcttatggtg gatgcagaag
    33961 aaatcactac tgtcatcgtc aacaaccgtc tgaccccaag gcagaatgtc aatctagagg
    34021 aagttctggg tgttaaggtc attgaccgta tgcagttgat tttggatatc tttgccatgc
    34081 gggctcgaag ccatgaaggg aagctccaag tccacctagc ccaactcaaa tacctcttgc
    34141 ctcgcttggt tggtcagggg attatgctca gccgtcaggc agggggaatt ggttcccgtg
    34201 gtcctggtga aagccagctg gagctgaacc gtcgtagcgt tcgcaatcaa atcacggata
    34261 ttgagcgcca gctcaaggtg gttgagaaaa atcgtgcgac tgtcagagaa aaacgtttgg
    34321 agtctagcac ttttaagatt ggtttgattg gttatactaa tgctgggaaa tcaactatca
    34381 tgaacatctt gaccagtaag acccagtatg aagcagatga gctctttgcg actctggatg
    34441 cgacaaccaa gagtattcat ctgggaggca atctccaagt aactttgaca gataccgttg
    34501 gctttatcca agatttgccg acagagttgg tgtccagttt caagtcaacc ttggaagaaa
    34561 gcaagcatgt ggaccttctg gttcatgtta tcgatgctag caatccttac cacgaggagc
    34621 atgaaaaaac ggttctctcc atcatgaaag acctggacat ggaagatatt cctcacttga
    34681 cgctttataa taaagcggat ttggtggagg atttcacgcc tacccaaacg ccatataccc
    34741 tcatttctgc caagtctgag gacagtcgtg aaaacttgca agcattattg ctagataaga
    34801 ttaaggaaat ttttgaagca tttaccctgc gagtgccttt ttcaaagtcc tacaagattc
    34861 atgatttaga gagtgttgca attctggaag aacgtgatta tcaggaagac ggcgaagtga
    34921 ttacaggcta catttccgag aaaaataaat ggaggttaga agaattttat gactgatatt
    34981 aaaactttgg ctctaaagta tggaggttat acaagtctgg acaaggtcta tctggatcag
    35041 cttctagctg gtaaaacaga gcaggagcag ttaacactta ttacacctcc gccaagtgtt
    35101 gttaatgcct actttgcaga actctatcag aaaaagagtc ctcaggctgc gacggattat
    35161 tttgcagaac tcagtcagga actgaatctt tacaatactg agccaagttt caccctagaa
    35221 aataagcctt ttattcggct taatctgtct ggtaaatcct ttggtttttg ttatgagagt
    35281 gacggccttg gtcggatttt ctctgaaaat gaggagaaaa tctcggatga cttgctcttt
    35341 gaaattgcgc aaattttccc ccatcaatta gtctttgaag agtctggtaa gatttacatg
    35401 aagcctgtcg aggacgagga agttgtcagc ctagaaagtc tcacagcttt gactgatttg
    35461 gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca gccaagagga tttattgcaa
    35521 gaagctgctg ctttttctgg caaacgctat ttccgatcgg aaaaccgcac agccatgtta
    35581 tatattgatt aattagaaag tatcgaatgg atattcaatt tttaggaacg ggggctggtc
    35641 agccctctaa agcccgcaac gtttcaagtc tcgccctgaa acttttggat gagattaacg
    35701 aagtttggct ctttgactgt ggagaaggta cgcaaaatcg cattctggaa accacaattc
    35761 gaccacgtaa ggtcagcaaa atctttatta cccatctgca tggagaccac atttttggtt
    35821 tgccaggttt cctttctagc cgtgcctttc aggccaatga agagcagaca gatttggaaa
    35881 tctacggacc tcaaggaatc aagtcatttg tcttaaccag ccttcgtgtg tcaggttctc
    35941 gtctgcccta ccgcattcat ttccatgagt ttgaccaaga ttctctgggt aaaattcttg
    36001 aaaccgataa attcactgtg tatgcagagg agctggacca cactattttc tgtgttggct
    36061 atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc taaaaaactc aaggctgctg
    36121 gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg ccaggatctt gttttggaag
    36181 acggaactga aatcaaggca gcagactata tctcagcgcc acgtccaggt aagattatca
    36241 ctattttagg agacactcga aaaacggatg ccagtgtgcg tctggctgtc aatgcagatg
    36301 tcctagttca tgagtccact tatggcaagg gtgatgaaaa aattgctcgt aaccatggtc
    36361 actcaactaa tatgcaagct gcacaagtag cggtagaagc aggtgccaaa cgcctcctac
    36421 tcaaccatat cagtgcccgt ttcctctcaa aagatataag caaactcaag aaagacgctg
    36481 ccacaatttt tgaaaatgtc catgtggtca aagacttgga agaagtggaa atctagcagt
    36541 cacagaaagg ataagtatgc ctactattct cattaccgga gctagcggtg gtctagctca
    36601 agaaatggtc aaactcctgc ccaatgacca actcatcttg cttggtagaa ataaggaaaa
    36661 attagcccaa ctctacggaa attattccca tgcagaattg attgaaattg atattaccga
    36721 cgattcagcc ctagaagctc tggtaactga tctttatctc cgctatggca agattgatgt
    36781 cttgattaac aacgctggtt acgggatttt tgagggattt gaccaaattg ctgataaaga
    36841 cattcaccag atgtttgagg tcaatacctt tgccctgatg aatctgtctc gtcaccttgc
    36901 ggctcgtatg aaggaaagca gcaaagggca tatcatcaac atcgtcagca tggcaggtct
    36961 aatagctact ggcaagtcta gtctttactc agcgaccaag tttgcggcta ttggtttttc
    37021 aaatgctctg cgactcgaac ttatgcccta tggagtctat gtgacaacag tcaatccagg
    37081 tccaatccga acaggatttt ttgaccaagc tgacccagat ggaacttatc ttaaatcggt
    37141 tgaccgcttc ctcttagagg cagatgcagt ggctaaaaag attgtcaaga ttataggcaa
    37201 aaataaacga gaactcaatc tcccgatttt gttgaaccta gcccataagt tttatactct
    37261 ctttcccaag ctagctgata agttggcagg ggaaactttt aattataagt aaaaagaacc
    37321 aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac tatcaaactg
    37381 aacttcttct agtagatagt cgataaagcg ttcgcccatc ttagataggc tggttttctc
    37441 atgctggata tagaccagct caatcgggtc atcaatatcc agtgggatag aaacaatatt
    37501 gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat ccaaaccaat
    37561 caagagatta aagagggtgg cacggtcact gaccacaatg gattttttgt ggtgttcttg
    37621 agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat agctgaggta
    37681 agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag ggttggtctt
    37741 gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg gctagcaggt gattgtcatc
    37801 cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc cgacctcact
    37861 gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa ggaagagttc
    37921 gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca caaaggcata
    37981 gtgttgagac gaaacgctaa agagttcgcg gtgggcgaca ggatttttat agcgttcctc
    38041 cagaagctgg gtctgctcga caacctgacg ggcataagag agaaactcca tcccatcacg
    38101 ggtcaaggtg attcccttgg gattgcggat aaagatctca atgcccattt cattttccaa
    38161 atctcgcact gcattggaga gacttggctg agtgataaag agttgcttgg ctgcctcatt
    38221 catggagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc tcatgttttt
    38281 attgtaccat acttgcctta gaatcgctaa agaagatagg aaaataagga gcattgtgtg
    38341 agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa aacattctat
    38401 gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa acgcttatat
    38461 gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt gatggataaa
    38521 ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga attggattat
    38581 attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca cttatactag
    38641 caatgttggc gtggatgggc tttaactatc ttcgtattcg ccgtgcggct aaaattgtgg
    38701 acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg cgcgacccag
    38761 cagaattcca cagaaaacat atccttggtg cacgcaatat tccttcaagt cagttgaaaa
    38821 ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgaaaac caacgtgcgc
    38881 aacgagttac aaatgcagct ctttacttga aaaaacaagg tttttctgag atttatatcc
    38941 tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct tatttccaaa
    39001 ttagtcgaat gataggatga agatattgtt ttatagtata attaatttgt ataaaattta
    39061 aggagttctt agaacatgaa agaaaagaag aaaaatccac tttttgtggg tatcttgtcg
    39121 attattcttg gtttgttatt tccaattgta ggcctgattt tgggtatcat aggattggtc
    39181 ttagctattt cataccaaaa agagtcacaa ttagactata aaatagaaaa gattcttaat
    39241 atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt aatttttcga
    39301 taaataaaag aaaaaagctt tagttttaaa gctttttttg ttttatcgac tcatctctaa
    39361 atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac cttctgctaa
    39421 atctgggttc aatgtgaagt ccccaaattg gattcttttg aggctagtca ccttaacacc
    39481 aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg tgatagaggc
    39541 ttgactgagg gagggacttg cagatagaat ctctagtttt gcgggtttac agacagtacc
    39601 atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta gaagtccatt
    39661 aacctcaact tgataagtct tatcgacatg atattgggga tggaggagtt ggaagcccaa
    39721 gggaccgtta tcggtcaaga gaaggagtcc tgttgtatct cggtccagtc ggccgacggc
    39781 atagagcttg tcagactgga tgtcagatgg aagcaggtcc atgacggtcg gaagttcctt
    39841 gtctttgttg gctgtaacga caccagcagg tttatgaagc ataagatagg tgtgttcata
    39901 gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga tattttgggc
    39961 tagggatcgg gctgggcaac tatcgacgag aatgtcccct ttgagtaagg cttgtttcat
    40021 ggcctttcgg ctgatttttt cttgagctaa taaattatct aaacgcatac tgtgcctatc
    40081 ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg agtgaaaaca
    40141 tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt tcagttagaa
    40201 aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat atccgtaaca
    40261 ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa ttattgaaac
    40321 aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac tcaaacgata
    40381 tcgaaaaaga gcgtgggatt accatccttg ctaaaaatac tgccgttgct tacaacggaa
    40441 ctcgtatcaa cattatggac acaccaggac atgcggactt cggtggagaa gttgagcgta
    40501 tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga accatgccac
    40561 aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc gtggttgtta
    40621 acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc ttggaacttt
    40681 tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat gcttcagcga
    40741 tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact atggcaccaa
    40801 tctttgacac gattatcgac catatcccag ctccagtaga taactcagat gagcctttgc
    40861 agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt atcggtcgtg
    40921 tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt gacggtacaa
    40981 ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt cgtgaaatcc
    41041 aagaagccaa agcgggtgac ttgattgctg tttcaggtat ggaagacatc tttgtcggtg
    41101 aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc gatgagccaa
    41161 ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa ggtaaatggg
    41221 taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac gtttcccttc
    41281 gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga gaattgcact
    41341 tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta tctcgtccag
    41401 aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt gtacaaatcg
    41461 acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt aaaggtgaaa
    41521 tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt gttccagcgc
    41581 gtggtttgat tggatactca actgagttct tgtcaatgac tcgtggttac ggtatcatga
    41641 accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga cgtcaccgtg
    41701 gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg tctatcgaag
    41761 aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg atcatcggtg
    41821 aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa atgaccaacg
    41881 ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc ttgacacttg
    41941 aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct gagtctatcc
    42001 gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag aagaaaaaat
    42061 cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg aattttactg
    42121 ctcctactct atgtatttgc gacaccagaa agcattaaag ggactgtcaa tatcgtcgct
    42181 atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct gaaaattttt
    42241 caattaccaa cagaaatatt cctagcaata gccatgttga tcctagctta ctttagtgtt
    42301 agagacatca cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag agagtttcgg
    42361 ctctcttttt gctaggaatt ttacaatagt ttgaattgct ttttagttaa cactatctct
    42421 tttacttgta tagtatgaat gggaaaaact tgaataaaat tcgttaatgg catctatttt
    42481 atatttggtt agtactggtt gcatattatt ttgaaataaa actactatat aatattattc
    42541 atagaggta
//
LOCUS       DAVKWS010000011        41790 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKWS010000011 DAVKWS010000000
VERSION     DAVKWS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.011"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(406..2133)
                     /gene="ezrA"
                     /locus_tag="VW852_000643"
     CDS             complement(406..2133)
                     /gene="ezrA"
                     /locus_tag="VW852_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV0271823.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2215..4161)
                     /gene="gyrB"
                     /locus_tag="VW852_000644"
     CDS             complement(2215..4161)
                     /gene="gyrB"
                     /locus_tag="VW852_000644"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV0271824.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4176..4739)
                     /locus_tag="VW852_000645"
     CDS             complement(4176..4739)
                     /locus_tag="VW852_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0271825.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4824..5378)
                     /locus_tag="VW852_000646"
     CDS             complement(4824..5378)
                     /locus_tag="VW852_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV0271826.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5389..6612)
                     /locus_tag="VW852_000647"
     CDS             complement(5389..6612)
                     /locus_tag="VW852_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0271827.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLACVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6649..9099)
                     /locus_tag="VW852_000648"
     CDS             complement(6649..9099)
                     /locus_tag="VW852_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV0271828.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SHSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9299..10228)
                     /locus_tag="VW852_000649"
     CDS             complement(9299..10228)
                     /locus_tag="VW852_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0271829.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10368..10655
                     /locus_tag="VW852_000650"
     CDS             10368..10655
                     /locus_tag="VW852_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV0271830.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10689..12023)
                     /gene="ciaH"
                     /locus_tag="VW852_000651"
     CDS             complement(10689..12023)
                     /gene="ciaH"
                     /locus_tag="VW852_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV0271831.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12013..12687)
                     /gene="ciaR"
                     /locus_tag="VW852_000652"
     CDS             complement(12013..12687)
                     /gene="ciaR"
                     /locus_tag="VW852_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV0271832.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12796..15342)
                     /locus_tag="VW852_000653"
     CDS             complement(12796..15342)
                     /locus_tag="VW852_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV0271833.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFFAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFVKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLTAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15465..15905)
                     /locus_tag="VW852_000654"
     CDS             complement(15465..15905)
                     /locus_tag="VW852_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV0271834.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15880..16749)
                     /locus_tag="VW852_000655"
     CDS             complement(15880..16749)
                     /locus_tag="VW852_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV0271835.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITALRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16727..17227)
                     /locus_tag="VW852_000656"
     CDS             complement(16727..17227)
                     /locus_tag="VW852_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0271836.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17238..17936)
                     /locus_tag="VW852_000657"
     CDS             complement(17238..17936)
                     /locus_tag="VW852_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV0271837.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17929..18195)
                     /locus_tag="VW852_000658"
     CDS             complement(17929..18195)
                     /locus_tag="VW852_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV0271838.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18261..19187)
                     /locus_tag="VW852_000659"
     CDS             complement(18261..19187)
                     /locus_tag="VW852_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV0271839.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNHPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19262..20077)
                     /locus_tag="VW852_000660"
     CDS             complement(19262..20077)
                     /locus_tag="VW852_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271840.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LHYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20081..20401)
                     /locus_tag="VW852_000661"
     CDS             complement(20081..20401)
                     /locus_tag="VW852_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0271841.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20698..20778)
                     /locus_tag="VW852_000662"
                     /pseudo
     CDS             complement(20698..20778)
                     /locus_tag="VW852_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20822..22819)
                     /gene="metG"
                     /locus_tag="VW852_000663"
     CDS             complement(20822..22819)
                     /gene="metG"
                     /locus_tag="VW852_000663"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV0271842.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22943..24202)
                     /locus_tag="VW852_000664"
     CDS             complement(22943..24202)
                     /locus_tag="VW852_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0271843.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24204..24905)
                     /locus_tag="VW852_000665"
     CDS             complement(24204..24905)
                     /locus_tag="VW852_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271844.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24889..26088)
                     /locus_tag="VW852_000666"
     CDS             complement(24889..26088)
                     /locus_tag="VW852_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV0271845.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVANEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26374..27720
                     /gene="gor"
                     /locus_tag="VW852_000667"
     CDS             26374..27720
                     /gene="gor"
                     /locus_tag="VW852_000667"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV0271846.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            27916..28452
                     /locus_tag="VW852_000668"
     CDS             27916..28452
                     /locus_tag="VW852_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV0271847.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            28508..28588
                     /locus_tag="VW852_000669"
                     /pseudo
     CDS             28508..28588
                     /locus_tag="VW852_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28657..28872)
                     /locus_tag="VW852_000670"
     CDS             complement(28657..28872)
                     /locus_tag="VW852_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV0271848.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28970..29290
                     /locus_tag="VW852_000671"
     CDS             28970..29290
                     /locus_tag="VW852_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271849.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29320..29658)
                     /locus_tag="VW852_000672"
     CDS             complement(29320..29658)
                     /locus_tag="VW852_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271850.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29670..30389)
                     /gene="trmD"
                     /locus_tag="VW852_000673"
     CDS             complement(29670..30389)
                     /gene="trmD"
                     /locus_tag="VW852_000673"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV0271851.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30379..30897)
                     /gene="rimM"
                     /locus_tag="VW852_000674"
     CDS             complement(30379..30897)
                     /gene="rimM"
                     /locus_tag="VW852_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV0271852.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30928..31716)
                     /locus_tag="VW852_000675"
     CDS             complement(30928..31716)
                     /locus_tag="VW852_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0271853.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIYDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPIFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(31820..32063)
                     /locus_tag="VW852_000676"
                     /pseudo
     CDS             complement(31820..32063)
                     /locus_tag="VW852_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(32293..32532)
                     /locus_tag="VW852_000677"
     CDS             complement(32293..32532)
                     /locus_tag="VW852_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV0271854.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32552..32824)
                     /gene="rpsP"
                     /locus_tag="VW852_000678"
     CDS             complement(32552..32824)
                     /gene="rpsP"
                     /locus_tag="VW852_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV0271855.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLTWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32994..33203
                     /locus_tag="VW852_000679"
     CDS             32994..33203
                     /locus_tag="VW852_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271856.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33682..33873
                     /locus_tag="VW852_000680"
     CDS             33682..33873
                     /locus_tag="VW852_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271857.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDF"
     gene            33987..34790
                     /locus_tag="VW852_000681"
     CDS             33987..34790
                     /locus_tag="VW852_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV0271858.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34847..36388)
                     /locus_tag="VW852_000682"
     CDS             complement(34847..36388)
                     /locus_tag="VW852_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0271859.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHDPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36390..36872)
                     /locus_tag="VW852_000683"
     CDS             complement(36390..36872)
                     /locus_tag="VW852_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0271860.1"
                     /translation="MVKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(36872..37957)
                     /gene="rlmN"
                     /locus_tag="VW852_000684"
     CDS             complement(36872..37957)
                     /gene="rlmN"
                     /locus_tag="VW852_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV0271861.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCSSGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(37978..38508)
                     /locus_tag="VW852_000685"
     CDS             complement(37978..38508)
                     /locus_tag="VW852_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV0271862.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(38665..39270)
                     /locus_tag="VW852_000686"
     CDS             complement(38665..39270)
                     /locus_tag="VW852_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV0271863.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39443..40480)
                     /locus_tag="VW852_000687"
     CDS             complement(39443..40480)
                     /locus_tag="VW852_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0271864.1"
                     /translation="MLAIEESQKLTLSNLPILSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNVIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40514..41449)
                     /locus_tag="VW852_000688"
     CDS             complement(40514..41449)
                     /locus_tag="VW852_000688"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV0271865.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT        12391 a         9111 c         7547 g        12741 t
ORIGIN      
        1 gggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatccgt
       61 gagttctcta gtctggagat ttttcaatag acttcgttat tggacggtta ccaagttact
      121 caaagtacag atttgaagct gcagataaga ctgacgaaat cactttaata ctaaatgaaa
      181 atcaaaaagc aaactaagaa gctagcctca ggctgtactt gagtacggta aggcgacgct
      241 gacgtgattt gaagagattt tcgaagagta taaaacactg ttttgaggtg gtggataaat
      301 cggctgggta aaaactcaat ctcaggagaa aatgaagtaa atctccccac aactatagaa
      361 aaagatttga ttctgctcct cacacaataa aatctttttc ttttattaaa aacgaatcgt
      421 ttcacgtgtt ttctcatatg aggtaacaaa gcgattggtt acaccaggct ctgccacttc
      481 caatgcttga gaaatcttgt caaatgaagc gtgataatca aattcttttt caaaaatatc
      541 taaagcttcg ttaaatgctt cttgaatgcg ttcatcaaat gagcgatagc ggttagaata
      601 ttgcaagagt tgctctgtca aagttgcata ttgtacaata ttataagttt ccgtttctaa
      661 agcttccata tcattcgttg caatttcaag aactcgggta acagattcaa tgttaatcat
      721 tttttgttct aactcaacca ttaaatcctc ggtattattg cttgccgtaa agaataactt
      781 caagaaagtt tgtggaatac ctggcagatt gcgtttttcc atgtatcgct tgatagtatg
      841 gagacgattg acataaacat tggccttttg acgtgcatta atatcatctt tctcaatttg
      901 tgtcaggcgc tcactaactg aaatttgctc atcttcaata tcttttagtt gagtttgtaa
      961 atcctcaaga ttttcttcaa gaactgaata agcttgggtt ggttcttctt gattcgaagt
     1021 tacctcaaca atagctgcct caaaactctc taattctgtc tgaatacgac gaacatggct
     1081 tgcagctgtc tcaggaagta aataggtctt gttcaaacgt gcaatatctt ctcccaataa
     1141 agtattattc tctttcatat gttgaaggta agttggaaga gttgcaagta gattttccac
     1201 tactttctga gcagcaattt ctcgagtaaa aatatcatac aaggcattga tttcctcttg
     1261 tgcctgtcca ttctcatatt cggcattatc caattccaac tgacgaatat tctcttggtt
     1321 tttcttgaat gcttcataaa gcaagtggaa acgcgcttca atatccgttt caacaaaatg
     1381 ataattagca tcaattagtt tacgataacc ggcttccaaa tcctgtaatt gatctggcaa
     1441 ttctgtagaa agcgtcgtaa ccaaggctgg aacacgatcc acaatatgac ttaaggccaa
     1501 aatgtgattt tctgtattat ccaaaatcac tgcggcttcc acagggtcac ccgatgaatt
     1561 caaggttaca aattgtgaaa attcagattg gatattttct aattgttttt caatttcatc
     1621 caaggcttga ccatactgtt ctgaattttc agcaactcta tgctgaagtt cctcaaataa
     1681 atccaaagca tgaagaacac gaccactatt tttagattct tgcttctcta agtctgccaa
     1741 agcattgcga attgccgcaa tatcttcttc aatcaaagta atttgactct caatttggtc
     1801 aatttgatga ctggccttga gaaaacgaaa tgaatggtta tagccttctg cttcaaagag
     1861 attattttca atatcggcaa aagagttgag agataaatcg acccattttt gattccattc
     1921 acggaaagcc acttgacttt gtccaatcaa gtgcatattt tttacagctt ctacttcatc
     1981 atttactgga agattgtata gttcttcttt tctttcttct agcgcctcta atctcccctc
     2041 gtttcgctta cgtagaaaga ttgccactac ataagccaga actaaaatga ctgcaattgc
     2101 aaccattaaa taaattagtt gtccattaga catatcaaac tcctttttta cttgaaacaa
     2161 tcgtaacgat tatatcatat tttttagacc aagggaacta cttctcccta ttttttagac
     2221 atcaagtgta ctatagacag cattttcttc gataaactca cgacgaggct ctactcgatc
     2281 ccccatcaac atatcaaaga ttttatctgc ttctgcagca tcatctacag aaactctagc
     2341 catcaagcga tgttcgggat ccatggttgt ttcccacagc tgatgatcgt ccatttcacc
     2401 tagcccctta taacgctgaa tagtcggttt ggtacgacct tcactataac gggctaaagc
     2461 ttcttggagt ttgatttctt gatctgcacc cggctggata tattctttaa tctcgcttcc
     2521 aaccttgaca ccatagattg gtggttgggc aatataaaca taaccagctt ctaggattgg
     2581 tttcatataa cgataaatca aggttaaaag aagggtacga atgtgggctc catcgacatc
     2641 ggcatcggtc atcaaaacga gtttttggta acgggctttc gaaacatcaa attctgcgcc
     2701 aaatcctgtt cccatggctg tgaaaagact acgaatttct tcgttagcta gaatcttatc
     2761 catacttgct ttttcaacgt tcaaaatctt accgcgaatt ggaaggatag cctgaaactc
     2821 acggttacga ccagatttgg ctgatccacc agctgagtct ccttcgacga tgaagagttc
     2881 tgtttcagca gggttattag aagaacagtc tgctagtttc cctggaaggt tggaaatttc
     2941 caaaccagat tttttacgtg tgacttcacg cgcacgcttg gcagccacac gagccttggc
     3001 agctaaaatc cctttttcca cgatacgttt ggcaatctgt ggattttcca tgaggaaatc
     3061 ggagaaggct tcactgaaga ggcgattggt aatcttgacc acttcgctat ttcccaattt
     3121 ggtcttggtt tgtccttcaa actgtggatt tgggtgttta actgagataa ctgcagttaa
     3181 gccttcgcga acatcttccc ctgttaggtt gtcttcattg tctttcagta acttattttt
     3241 acgagcataa tcattgataa cacgtgtcaa ggctgtacgg aaaccttgtt catgcgttcc
     3301 accttcatgt gtatgaatat tattggcgaa actcatgaca ttttcatggt aaccagttgt
     3361 gtactgcatg gctacctcaa ctgtgatatc atccatctca ccgtctgtat agattggtgt
     3421 atcaaagatt acatccttgt tctcgttgat atattcaacg taactagcaa tcccaccttc
     3481 ataatgataa tgcttggttt gttccaaacc ttcacgctta tctgtaattg aaatttgaag
     3541 accgcgattt agaaaggcca actcttgaat ccgtttattt aatttatcaa aatcaaagat
     3601 tgttgtttca gtgaagattt ttgggtccgg tgtgaagtga acagttgttc ctgttttatc
     3661 cgtatctcca actatttcaa gatctgcgac aacatgacca cgacggtatt cttggtaatg
     3721 aatcttacca tttttgtgaa catgaacgtc taattgagtg gaaagggcat taactactga
     3781 cgaccccacc ccgtgaagac cacctgaaac cttgtatcca ccaccgccga actttcctcc
     3841 agcgtgaagg actgtaaaga cggtctcaac agcaggacgg cctgtttttt cctgaatatc
     3901 gactgggata ccacgcccat catccacaac agtaatcgaa tcatctggct caataaaaac
     3961 ttgaatatgg ctggcaaatc ctgccaaggc ctcgtcaatt gagttatcaa caatttccca
     4021 gactagatgg tgaagacctt cttttgaggt tgatccaatg tacatccctg gacgcatacg
     4081 aacagcctct aagccctcta aaacttgaat ttgactggca tcataatcct gtgcctgcag
     4141 atttttgatt tcttctgtca tcttattcct ttttcttata tgtctaatac ttgtcttaaa
     4201 ttcacgatac tggtaaacaa gtgggtatca agtcctgcag cttgacctgc ttcgatatca
     4261 atcggccgat caccaatgac aagaccagag ctaatctgat acttttctct taaataaagc
     4321 atggattcgg gatttggctt tctcttaaag cctgagctag aagtcaccac ttctgtaaaa
     4381 taagctgcta tagaggtttt ttctaaaatt tccaaaacct gatcatttcg atgagagacc
     4441 aaaaaatgac ggccaccttg atttgaaatg acttccaata ggtcagaaac tccttcaaat
     4501 aaaatcgggt gttcaagctc tctggcttca ttttccttgt acttttctaa aaaattctct
     4561 aaattgggag cgaatgtctc aatcgcaaaa ggagtagaaa cctttaaagc ttgatagaca
     4621 ctgtcatggt cttgtgtgat accatacagt gccaatgttt caacaaatgc agctgttgaa
     4681 gtttcataat tatccagtaa agttccacct aaatcccaga tgtaatcgtg atatttcata
     4741 cccttcatta tagcattttt ttatgaaaaa agcgatgatt tccctgagtt ttccacataa
     4801 aaaacgagag aataactccc gttttactga tttttaccaa agacatctcg atagagcatt
     4861 cctgttcgta aactctctgc gtcccctcct agttgctcca ccaactcgta ggcaaaggca
     4921 agggctgttg aaggaccccg actggttgtc aactgaccat ctaccactac tgtttccttg
     4981 acgtagtgac catcaaggat ttgctcttga acgccgtcat aacaagtgta tcgcttattt
     5041 ttcaatatct ctgcttgatt gagggcaatt ggtgccgcac aaatggctgc tagtttcttc
     5101 ccttcttgct cgaagctttg caattcttga atcaaggtct gattatcacg taaatgtgca
     5161 gaaccaggca tacctccagg aagaacaatc atatcatagt ctgataaatc tccatcaaag
     5221 acatgatctg ctcttacttg gattgcatgc gaacccgtta cttgctcttc aaaaccaacc
     5281 atatcacatg tgatattggc tcgacgcaag acatctacaa ctgtcaaggc ttcaatttct
     5341 tcaaagccct gagctaacat aactgctact tttaccatga ttttctcctt atttaatttg
     5401 ttttaataca acctttttcc gtttgaatgg gacttttccg ctcttttctt cagctagatt
     5461 agtctggtaa ctcatcgata aaagcaaacc aacaccaatc agattactga taatagctga
     5521 tcccccttgc gaaatgaaag gcaagggaat ccccgtcaaa ggaagtagtc cagtcacagc
     5581 accgatattc tcaaagatgt ggaagagcaa catcataatc aaacctgtgg aaatataagt
     5641 gtagaactgg ttatttgatt taagagtaat cttcaacata cggtaaatca acatgagata
     5701 gagggcaata accaggacag agccaataaa gccaaaatct tctgcaataa ccgtaaaaat
     5761 catatctgac tctcgaactg ggataagcag attcgaagca ttaaatccct gaccaaataa
     5821 gccaccactc ccaatggcaa tctgcccttg agcctgctgg taagtcgttg tttgggcaaa
     5881 ctcaaaggga ttgagccaag ccaaaatccg attaatttgg taggtcggca ttccaatctg
     5941 gtgaagaaaa gctcgtccgt ccttgctaat aaagatagct aagaaaccag caactcctgt
     6001 tacagcagtc acaaatactg ggataataat tttccaagaa acccctgata ataaaacgat
     6061 tcctgagaaa atggctacaa aaaccaaagc cgtccccaag tcactttgaa gtgctaaaag
     6121 aactaggact ggaatggtaa agagaatcat ccagaaaatt aacaaaaagt ccagcggaac
     6181 cgtgcgtctc cattccttat gtttctttgt aaattggaca atgacacaag ccaacatgag
     6241 gatataggat atcttcataa attctgacgg ttggaatagg gtaattccat ttattgatac
     6301 ccagtttttg gcacccgttg atgcaactaa gcttggatta taaaatacaa tcggcaagat
     6361 cataagtccc aagcctaaaa tatatagaaa gggggtcacc ttccaaagaa attctgtatt
     6421 aaagagcatg accacaaaac caatcacaag ccccaaggca atccaggcga cctgctgccc
     6481 taaaatgggc agaatattat tgggataatc atgactaacg gctatataga tagccaccac
     6541 accgatgacc agtagaaaaa atactggcaa gagcaaactg taatcgactc ttgagtcgag
     6601 agaacgtttc atatatacta accttatact ttcatacaat actatttatc aaagttcatt
     6661 aaaaaatcta tcaatagcct cgtcaacttc ggatcgagag atggttttaa cagtcgcttc
     6721 ttctgctaga gatgctacta tttgtttgcc gtatcgtttt ccgacgattc tcctatccaa
     6781 aataagagtt aaggaacgtt ggtattcacg tctcatactt cttcccaaag cctgttttaa
     6841 acgaataatg gccattggca attgataatc ataaaaggca tttttccctt cttgattcag
     6901 ttcttgatta atctttttcg tcaagggttc ttgaggattt tggaaaggaa gcctcggtac
     6961 aacttgaatc acaaaaggat ggcttgaaaa atcaactccc tcccagaaac ttgctgcacc
     7021 aagcaagatt tgttgttcac ctttttcaaa gcgtttcttt agctgatgaa catccccatt
     7081 tttatactgg gccaagtggc taactgtaag taaatccgat actgctagaa gcatgtcttt
     7141 agcggtaaag agaaccaaaa tcggttgctg gaaagcttga atttccacta gtaaagcagc
     7201 tacctctctg gcatagactt ctaaggaggt ttctgttacc agagggaaat ctttgaccat
     7261 gaccacttct tgttcctgtt ttccccgaga ttcaatcttg acaaatttag cttcaggata
     7321 gcctaaaagg tctgccaaag aaaccctctg actaatctca agagtagccg atactcccag
     7381 gacttggcaa ctctcaggaa gtaaagagga cagaagagta cggcctgatt ttgtagaaga
     7441 aatctgaatt ttcttttgac tcgtttcagt tacttcaagc cagtaatcac cttcagctgt
     7501 aaaatagcga accagctcat caaagccttc tacttctaat tctgaaaaat actgatggag
     7561 attatccaga gaatctaaga tatttttcct agatttgcca gactgaaatt gttctatcaa
     7621 gtagagacac tcaaagcgaa tactttctag tatccgttgt tgaaccctgt tttcttctcc
     7681 tactaaagcc ttatcaatta aatcgataat agattgtata tcgtaggtct cttgaagcag
     7741 attttctaga gctaacaaaa tcttttggac ttcatcaata atcagtaaac ggtcactgac
     7801 aaattcagga ttatcttcaa gtctggtcac gagataggca tgattagtca ctaaaagctt
     7861 gcaagtctct gccctttctt gactacgttt ccaaaaatct tccgtcacaa ataagctatg
     7921 ggatgataaa ttcccatcat gacgaaggtc tgctagaaaa tgttggtaac ggtagagttg
     7981 cccgatttca tccaaatctc ctgtctctgt ctcagtaagc cagaccaaga cttgcatttt
     8041 aaagcgtcta aataagcgat tttcatcatt ttcctgcaag gaatgataaa aggcatccaa
     8101 cttcagataa ttttgtggtc cctttaagct atgaatatct gtatggaaca cttccttgag
     8161 acgtttacct tcttcttcca tgatttgatt ttgaagaatc tttgtcggaa cactaagaac
     8221 aatttgtcgc tctttggatt gagataaagc gggtaagaga tagccatagg ttttcccaat
     8281 ccctgtcggc gcttgaatca gagagacagg ttcatctttc aatagcaagc caacctcttt
     8341 agcaaaactt tcttgttcct ccctcacttc aaggttcaac agagaaatat ttttagaaaa
     8401 gtcttgagat agttttcgta gctccagaga agctgccgtt ttcttaaaat acagaccttg
     8461 aacttggacc aagtctggag aactcaggat agattggttg cgataagttt cctcaataac
     8521 caggtaggac tcatatagga gagcgtcagc catttccagc aagcgttcca agagaccttt
     8581 aggaagctgg gtcatctttt cccgtaaaaa aagaagtaat tctgctgtag cttgggcatc
     8641 tgaaagggct gtgtgtgcgt gtttaagagg aattcctaat tctcgacaca aaatcggcaa
     8701 gctatatttt tccagttcag ggaaaaagac ctgggccaat tcgaccgtat caacacgagg
     8761 gtttcttagc tcatagcctt caaaaaataa attttccgcc aagagattag catcaaactg
     8821 aacattatgg gctacaaaaa tcccatcctc caccaagtca aagatttttc tggcaacttg
     8881 cgaaaaatca ggtgcttgcg ccagacgttg gtctgtcaat cctgtcagtt ctttgatatg
     8941 agcatccaag ggttcatgtg gattgacatc cgtcgtatag tgatcgacga tttctccatc
     9001 ctcaatcacg acaattccca cttggataat tttagcctta ctacctgtgc tagttgcctc
     9061 taaatccacc acaacatagc gattaccaat cgttttcatt ataaaaaatt ataccaaaaa
     9121 aagggccatt tggcacctgc aaacatagga taattttcaa actcaagaac actacttttc
     9181 ttagaaatcc catcgaatag agcattctag cctgctgatt tttttaaaat agtgaagaaa
     9241 tacgaaaaat tgagagaaga aatgttttca tcttccttct ctcaacgaat catgtatttt
     9301 atttaaccat gtcaggatcg tagtcataaa atcctgtatc aaggaaacgg tttttagggt
     9361 gaagtttacg aacttcttca tccagcaaga aggcttggtc atggctaaag ttgccctctt
     9421 gaatcaagct acgcgcttgg ataaagagtt ttgcaatctc aacaaagttc tgaccaggag
     9481 tgtagagccc ttctagtttc cagcgagtaa agccatgctc caccaattct gttaatttga
     9541 tcatcaaatc aaggtcattg ttggcaaaga tatgggtccc atgattatct tcaaaaatgg
     9601 aatagtggct ctctggatca cttggctcag ccaagaagag gtcacgttta tgcgtctttt
     9661 catcatcgat atgtgtaaag ttatagtagt tttgcaagag tggacgttta gaatgatgga
     9721 tgacgctagc accgtaaacc aaaacttcag caggaatttc caaaatctct ggcattttga
     9781 aaagttcagc tgatggaatt tcacgcgcca aaacagcctc agatgcgcca gccttttgtc
     9841 cccagaagtt aatctgacga ctgctagtta ccatggttga agcatcgtag atggtcttaa
     9901 atgaataacc atcgcggtta actacgtaaa agacgcctgc atccccaatc gtaatatagt
     9961 ctgtcttgat ttcttccaag aagtctaaga aaggcttgat acggtccatc atatcttggt
    10021 gcatgagagc attgaccgca acgagcaatt ccttaccagc atcatgaacc aacttagcga
    10081 tttcacgtaa ttggtcataa ctaaaggtcg ttggcagacg aaggccaaaa tctttctcac
    10141 cgacatagat acggtctacg ccagcttcga gtagttgttc aacttgttca atactttcag
    10201 cagttgctgt aatgacaatc ttttccataa gaaaaattat accacatttc tccaaaatca
    10261 tccatgcttt gaaaatgaaa agggacttta ttctttttgt aacctttctg taataatttt
    10321 ctgccgaaaa aatgataaaa taggtatgta ctgttaagga gagaatcatg cccgtaagaa
    10381 aattacaatc ctatgaggta gactatcaag aagaattaaa ccagcagctt cctcattacc
    10441 aagcttatac acctgaagcg caatctgatg ccaatctcaa ggaaatccta ttttttatta
    10501 atatcgctgt tttttgtatc tgtattgcta tctttagttt tatcttttta gcattaaaat
    10561 tatcaactgc tcttgccttt gccgcagcaa tcggattcag cttacttgtt ttaaaagttc
    10621 aacggtctat tatcaaacga aaacgtagaa gataaatcca aaatatcgcc ccagttggag
    10681 cgatattttt attttttctt tttagatggt gtctgaatgg caatcttcac ttcaaagatt
    10741 gttcccttgg gtttattatc tttgacagta acagttcctt ttagagcatc tacaatttgc
    10801 ttggctaggg ataatcctaa accaaaacca cctttttgcc gggttctagc tttgtctact
    10861 cgataaaaac ggtcaaaaat tttcttttta tcttctgtcg aaataccgat tccattatca
    10921 gaaacaagta aataaagatt gcgatcggtc gccgagataa gaaaatcaat ttcaccatcc
    10981 tcctcagtat acttgacggc attatcgaaa agaatggtca tcagttgttt cagaagaagc
    11041 tgatctgtga caattgttcg atggatacga ttttcaaaac ggaagacacg attattttcc
    11101 gaagcaatca tctcgtagtt tgtgaaagtt gtattaaaaa agctagttgg aacttctgca
    11161 agctccggct taatcccatc atctctccga gctaagttca gcaagcttgt cgttaaaaaa
    11221 cgcatatttc ggacttcttc caaactcgat gcaatgcttt cgctcacatc cataatggta
    11281 gcttctggct tacgaaaaag ggtctctaag cgattttgca aaactgcgag tggagttcgt
    11341 aactcatgac tggcattttc cacaaaagac tgttgcttct gcatactctc aagcaggggc
    11401 ctaacactga ccctagctag atagagactg gcaagtaaag acaaaatcca gaaactagcc
    11461 atcacgacca caatcaattg ctcatgcttt tgactagcct gctccaactg actggtatta
    11521 atcaagacag cagcatactt gatattggtt gaaaccgaac taatattggt ctccatcaaa
    11581 atcacacgat agatttcttc ctgcccatag ctattaaaaa cctgaatctg gtagatatgt
    11641 cctagttctt tcttttctaa cttaatctta tccaagccca aaaatcgatt tccagaaaga
    11701 agttgagtaa agtctttatc aaagagaatg acttccgtat tggaactgac attaggtttt
    11761 atttctactt tactagcgtc cgccctagca ttttctaaat ctttaatctc ttctgttgcc
    11821 ctatttatag ccagctgaat aactgcttga ggattttcac tcagtccatg aagcttatcg
    11881 tccaccgaag tataaagact cgaatgcatg acttgtaaaa taatcagagt cattgtagaa
    11941 aaaatcaggg tgaagacacc gaagttgcgg ataaaataac taaagtcatc cgcataccat
    12001 gtttttttaa gtttactgaa catcttttaa aagataccca acactacgca aggtttgcaa
    12061 attctctgca aaagtggttc cctttaattt cttacggact tttgaaacat agacttcgac
    12121 aaccgaaatc gttgtatcac tatcaaatcc ccatagacgg tcaaaaatct gcgtcttagg
    12181 caaaatcaca ttttgatttt gaaggaaata aactagtaaa tcgaactctt tccccagcaa
    12241 ttcgacagga gtatcttcaa ctttaacggt attggttgat aaattaacca cgatattccc
    12301 ataagtcaag gtgttttcat taaacttccc tgaacgtttg agaagggcct gaatccgcat
    12361 tttaagttct tctaggtaga aaggtttggt cagataatca tccgctccca gttcaaatcc
    12421 atgtcccttg tcatccaaac tttccttggc agtcataatc agaactggtg tcgtaattcc
    12481 cttttcacgc aattctttta agacttggaa accatttttt tctggcaaca tcaaatccag
    12541 caaaatcaag tcatagacac cactctcagc ttcgtagaga ccttcttctc catcaaatac
    12601 ctgcataaca tccgcaaaat cgtctaaaaa gtcaaatact gaatttgaca gacctaggtc
    12661 atcctcaacc aataagattt ttatcatgag aaactcctcc ttattaaaac tattatacca
    12721 aatttgcctt aaaaaaaact caactctctg cattttacat gagatagctg agttttcttt
    12781 ttattttagg cttatttatg catttccgta ttgaagaaca actgcttcga ctgcagcttt
    12841 ttcacggcta atcaagtcaa cacgcgctgc aatttccttg attcccatac cgatgttacg
    12901 gctaagagca aggtcagaaa gttgcggttc aaagaactcc ttgtattccg ccaagcgttg
    12961 ctgagtctta aatacatgag caggaaggat aacaaagcta tcaaagctca tatctcctcc
    13021 aagggctgcc ttaatccaag cccagttttc acgcgcccaa gaccaagctg ttttctgagt
    13081 tgcttgatga gctaggaatt ggtaatacca agcagacaag tcctgtggtt tgaccacaaa
    13141 tttgtccttc caagaagtaa tcaagttttg gatattatcc gcatctgtac tgtatgcaag
    13201 agctgctgtc aactggcgtt taaagacagc atctgttgcg tgagtataag tatcaagata
    13261 aagtgctaac aagtctttag tctcatgatg tttcatctca ttaatcagaa cttgtgaacg
    13321 aatagctgct gggagttctg caagattctc cttgtgtgtt gcgaagattt ggctagcgac
    13381 ttgacgagct tctgcatcat ttgagcgaat catcatcgaa acagccagct gacgaaccaa
    13441 ttcatcctca tctgattctc cgtctttagc ttcaaaacca agacggtcat agttatgacg
    13501 agccaattta gcaaccagtc ctttgaaggc tgtttcagca tccgttcctt catcaataaa
    13561 gcgctcaagg gctgaaatca cttgagaaac agctgaaacc accagataag actcttcctt
    13621 agcaagttta tcaagaactg gaagcaagtc tgcataagaa atgtgccctg cctcagccaa
    13681 caaacgacgt tcttgaacaa tttgcagttt gcttgtgtta tcaagtgtct ctagctcagc
    13741 aagaacagct tctaacaagt ctccttgata gtcggtaata tagtgggcag tattttcagt
    13801 gttgagacga agagctcctt cattttcagc aagaagagct gcgtagccag ggatttcgat
    13861 actttcagtt tcgagtgtat caggcaagcc tttccagttg ctattgaggg gcaccaccca
    13921 gagacggttc ttgtcttcgt tctcaccgat gaagaattgt ttttgtgaaa tcttcaagac
    13981 atcattttca actttgacag taagaactgg gtaaccaggc tgttccaacc aagaatccat
    14041 gaaggctgcg acatcacgtc ctgacgcttg accaagggca tcccaaaggt cactaccaat
    14101 ggtgttgctg tattggtgtt tttcaaagta ggcatgcaaa cctttaacaa aatcagcatc
    14161 tcctagccaa cggcgaagca tgtgcatgag acggcttcct ttggcataga cgatagcgcc
    14221 gtcaaagagt gtattgattt catctggatg tttaacttcg acgtggacag actgaacgcc
    14281 atcagtagcg tcacgttcaa gagcaagagg tactccacct gtttggaaat cttcaaagat
    14341 attccagctt ggttcgatgg tatccacaca gacgtattcc atcatattag cgaaactttc
    14401 attgagccaa aggtcatccc accatttcat agtcacgagg ttcccaaacc attggtgagc
    14461 caattcatgg gccacaacaa gggcaacttg ttgacggcta gcaaatgtag agttctcatc
    14521 gacaaccaag taaacttcac ggtaggtcac aagaccccag ttttccatag caccagctga
    14581 gaagtcagga agggcgatgt ggagagattg aggaattgga tacttaactc catagtaatc
    14641 ttcgtaaaac tcgatagagc gaacagcgat atccagtgag aaatcaagat ttgaaagtgg
    14701 atgtgctttg gttgagtaga cacctaccag ggtaccattt ttagttttag cggtcacccc
    14761 ttgcaaatca ccagcaaaaa aggccaacaa gtaagaagac atgcgaggtg ttgtctcaaa
    14821 cttccagata cctgtttcct tacggttttc aacatcgatt tctggcatgt ttgacaaggc
    14881 caattcacct tctgcttggt caaagcgaag agagaggtca aaagttgctt tggcttcagg
    14941 ctcatccaca catgggaaag cttcgcgcgc aaaatggctc tcgaactgag tagacaagac
    15001 ctccttcttg actccatcaa ctgtataata agaagggtaa atccctgtca tgttgtctgt
    15061 aattttacca gaaaaggcaa gaaccaattc aacttgacca gcctcagcca attcgatatg
    15121 aagggcttca ttgtcatggt caactgtaaa tggacgagct tgacctgcaa cttctacaga
    15181 ggtgatttcc aagtcttttt ggtggaggga gatgcggtca ctctgtgctt gaccagtgat
    15241 ggtcactttc ccagaaaaag tcttggtctc acgactcaaa tctaaaaata aatcataatg
    15301 ttcaggaaca aattgcttaa taaaatgttc aactgcttgc atagttttct cctattctaa
    15361 gtttaagagc tgctgctctt cttcaaacaa acttattata tcatattttg cttgaaaaaa
    15421 aaggattgga cagcgatttc caaaactttc ttccttctaa tagtctacag tgggtagacc
    15481 ttgcgaaatt cttctaaaac aaccttgctt tcagatgtaa aagtcagccc tacttcaccc
    15541 aaacagtctg tgaaaaagtc ctcatgaacc tggcgccaat aggcaagtga tttgtctccc
    15601 tcaccttcct taaaggcatg ttgcgcagaa acttgattga agagttcaac agaaaccttt
    15661 gtaatttcga caatgcagac agactgattt tgactatcta aaatgatatc gaaggtccct
    15721 acttggggaa gtggttcgtc ttctagtaca tagaggtcat aggctgatgc tgttgctgtc
    15781 ttttctcctt taaacaccaa atccgctaaa aggtctggtt caactccaaa agcccaagca
    15841 tcgatttcat ctccgatcaa aggattgatt tgcttgtatt tattccacat ttcttgcggt
    15901 atcatgggta ctcctttgta attttttact ttcttctttt atgtgtttaa gatgatctgg
    15961 atggtcaatc tctaaatcaa aaatctctgg aatagaactg tagtggataa tgcacttgat
    16021 acccaactga ttcatttttt gtatgaaaga agtattcaga tagcctgcta cagcaaaatt
    16081 aatcttgttc tttcttgctt tatcctgcat atctcttagc atatctaaca ttattggact
    16141 ttccagatca tgccattgac tgtttctcat agtcgcaaaa acaaaggaag tcaaatcatt
    16201 cattccaact acaatctttg aaatgcccgt ttccagtata ctagataagt caaaatacgc
    16261 tgacggtaat tcaatcatcg ttccgacttt cccagtaaag ccatactgac gcaatgctgt
    16321 aatagcttgt tttaattggt cggcatcatt gacaaaagga aagataacag atagattggg
    16381 gttggtttga taaacttctg taacgacatg tgcttcagcc tgaaattcat ccaaacacgc
    16441 cagtaaacgc ctggttcctc tatagccaaa caagggatgc ccttcgtcaa aaaactcttt
    16501 agtccccact aaacaattgg cttctgtatt cgttaattca gtaaaacgat accaaacttc
    16561 ttcatctgag tacagggagc agatagtctc tagataatct tttacaaatt gctgacaact
    16621 tgctaatatg atgttttgat taagctctct caacaaatat tccccacgaa tcatgccgac
    16681 gtggtgaaat agttgaggat aaattttttc aagaattttt tcgccactaa gggcaagttg
    16741 atttctcatc attcaccttc caattcatgt aagaagtctt gtccagttct ggaaatccta
    16801 ataattcaga cttaaccttc aagactaatg gcgatgcatt ttcttctgta atctcttgaa
    16861 tatccatcca aatatatcca agtgaatcat tcgcaccatc agacacagct tccgaagtcg
    16921 taacttgagg tgcactctca ttcatttcaa catcatacaa ggccatgaca tggtgaacca
    16981 taaaatttgt taactcttct ctgacgaaaa catcgtagat tcgaggatta gagtagcttc
    17041 taacagtaaa tcccgtctct tccataactt ctctagtcag cgtttccgtc agtccttcac
    17101 caagttgctg actgcctcca ggtagatcat accgatgttg ataagggcct ctcgtttttt
    17161 caatgcagag taacttccca ttttctaagc aaacagcgta gactccaaag tgttttttga
    17221 tttccatcca actcctccta cttcaaagac cagccaccat ctattgtcaa gatttgtcct
    17281 tgcatggcgc tcgcttttcc acttgctaaa aaaagactaa gctctgctat ttcctctggc
    17341 tcaatccagc gcttgattgg ggtttcacta gccacccagt cagccaaacc acctggttca
    17401 aaatccgcag cggtcatagc tgtcttgact gctcctggag cgataccaaa gacctgaatc
    17461 ccagcttcag catagtctag agccaactgc ttggtgaagc cagccaaggc atgcttggat
    17521 gaagtatagg cgtgaccacc tccacctgct aggctagaag caatggaaca catattgatg
    17581 atgattccct ttttattttc cagcatttgt gtcaaataat accgagtcaa ctctactgga
    17641 ataatgtagt tgatttcaaa aatctcttga atgtcctgcg ccgtttgttc caacagtggt
    17701 ttgtaatcat ccaaaactcc agcagtatta cacaaaacat ccacctgagg gcaccagtca
    17761 aaaataggtt ccaagtccaa ggtcaaatct ctctgtaaaa agtgaaaatc accctctaag
    17821 agtggctttt caccttggtc aactccataa acttgatagc ccttctctaa aaagaggcga
    17881 gcctgagcca atccaatacc tgaactcact cctgtaatga gtacacgttt agtcatgcac
    17941 ttctacccaa tccgttgcca aaacatcaca aactgtcggg ctccacatgg aaaaaccttc
    18001 tccttcgcca gaaacgttga ttaggaaata aggtgtcatt tcaagtgcaa gcccattttg
    18061 ctcgatggta tcaaagagtt ggacatagtt ttccgcacct ccccaaccag ttcgtacata
    18121 ttttctctta gcctttaacc caggcaggat ctcttcaaat gtcatgtttt tctcctttaa
    18181 ttctacattc ttcatttaat tatagcaaaa aaccgcttta tacggctttt tgaatgtgag
    18241 ttattcaaac ctgctactac ttacggcaaa ttattccctg cagcaagata aatttcatac
    18301 cattcttttc ttgttaagct aaagtttgcc gctcggctaa cttctctcaa gtgcttagga
    18361 tttgttgtac ctacgactgc ctgcattttt gctggataac gcaatatcca agaaatggca
    18421 atagttgaag aggttactcc atatttaata gctaaacgat caagtacttg atttaaagct
    18481 tgaaatttct catttccaac aaaattccct ttaaaatacc cgaattgtaa gacagaccat
    18541 gcttgaatga ccacatcgtg taattggcaa tattcaaaaa tgctgccatc tcgcatagct
    18601 gcttgactat cttccatatt aacatgaaaa gctgattcaa atcctggagt aaaagccgca
    18661 ctcaattgta gctgattaac agctaacggc tgcttgacat cttttttaag caactccatc
    18721 atcataggat tttgattaga aactccaaaa tctcgaactt taccttgttt ataaaggaga
    18781 ttaaaggctt ctgctacttg gtcagattcc atcaaagcat ctggtcgatg aaggagcaag
    18841 ctatctagat gatcaatctt caatctttgc aaaataccgt ctactgattt tataatatag
    18901 tccttagaaa aatcaaaata ggtaaattct tcaatgcgaa tgccacattt ggactgaatc
    18961 cacatctttt ctcttaaatc tggatgattt tttaggacaa gacctaacag ttcttcacaa
    19021 cgaccacgac cataaatatc agccaagtcg aaggcattga ttccaacaga aagtgctgtt
    19081 tctacaagct cttcaacttc ttttacagat ttatctttta ttctcatcat tccgagaaca
    19141 atttctgata attctttgtc atcttgacca agagttatgt atctcatcaa atttttctcc
    19201 tttaatttct aacattcttc ccttcattat aacaaaaaac cgctttgcaa cgactttttg
    19261 actatacttc actccatttt atcttcttaa acccacggaa caagacaaag attccaataa
    19321 agaggacagc taaaggaata acttttgtaa ggaaaacatt tgaaattccc atccactcat
    19381 aataacggag cagagaaccc accacaagat gggcaataat catactgaca aatggacgaa
    19441 agaccgcttc tttccaattc caaataccga taactagcga aatcgtaaag atagacaaac
    19501 tatcccaggg agccggaata taaaaggctc cttcttgtat gaagcttgcc attcctacat
    19561 atcctaaaac aactagaaga actatagtcc caacaacaat gtaagtgcca attttcattt
    19621 taggagaatc ttggactaaa cttcttcgta aaattgtggc cacaagtcca aatccaatca
    19681 gaaaaataag aagttgccct aaaaatgtga gcaaattgac tgttaagaga ggacctttag
    19741 aaaaatcact tagtagttga taataatgta ataccgccag gacaagaatt ggcgtcaaaa
    19801 gggactcttt gatagaactg cgaggtgctc ccttgagaat ctctttcatt atttttttag
    19861 gattcttacc tagataatcc tctgcactca tgccatctcg ttctgcttct gagaaatcta
    19921 gcatcatcaa atagatctgc tctctgagat agtcttcatc atagagaaat ccagcaagat
    19981 taaaactttc ccacaactcc tcaaaatact tttgattctc ctcagaaaac tcatgtagca
    20041 aagcgcttgt ttcttcgtaa tacttcattt tcttcatggt ttaaccccca ttcttaatcc
    20101 cttctacttt ttgactcaaa tcgtcccatt gttgccaaaa gactgagaca cgctcttctc
    20161 cttctttcat taatgaaaaa tacttccgat ctggaccatc tggcgacggg cgcatgtcgc
    20221 ctcttatcca ttgatttttt tctaactttt gcaacaaagg ataaatagtt cctggaacga
    20281 tagtatcaaa tccagcctct cgcaaagtct gaaccaactc ataaccatac cgctcttttt
    20341 gaccaatcat atccaagaca caaccttcaa gaacaccttt taatagctga gtttctttca
    20401 tcacttctcc cttctaatct attttgtaat acctactagt aacttcacct atagtatatt
    20461 acttctacac tagtttgtaa agcataatag ttaatactct tcgaaaatct cttcaaacca
    20521 cgtcagcgtc gccctaccgt atgtatggtt actgacttcg tcagtttcat ctacaacctc
    20581 aaaacagtgt tttgagctga cttcgtcagt ttcatctaca acctcaaaaa catgttttga
    20641 gctgacttcg tcagtttcat ctacaacctc aaaaacatgt tttgagctga cttcgtcagt
    20701 tctatctaca acctcaaagc agtgttttga gcaacctgcg gctagcttcc tagtttgctc
    20761 tttgattttc attgagtata aataaaaaaa cagaactagc ctgaactagt cctgtctact
    20821 tttacccaat cacacttcca tttggtacag ctggatcaac tgtgagaagg gttaatttgc
    20881 catcatgttc agctgagaga atcataccct ggctgacata ttttttcatc attttacgtg
    20941 gtttgaggtt agcaacgatt tgaactttct tgccgaccaa ttcttgttca tttggatagt
    21001 attttgcaat tcctgaaaga atctgacgat cttctccatc accagcatcc aagcggaatt
    21061 gaagcaactt atctgaacct tctactttag acacttcttt gacttctgcg acacggattt
    21121 caaccttgtc aaagtcttca aacttgattt catccttgtt tagtttgagc tcaacttcgt
    21181 ccggattcca ttctttttcg actgctggtt tattgccttc catttgttcc ttgatatagg
    21241 cgatttcttc ttccatattt agacgtggaa agataggtgt tcctttggca actacagtca
    21301 catctgctgg gaagtcagcc aaactcaagt tttcaagact agaaacttct tccaaaccaa
    21361 gttgagtcaa aactgcacga ctagtttcca tcataaatgg ttcaatcaag tgagcaacta
    21421 cacgaatgct ggctgccaag tggctcatga cacttgccaa ttggtcacga agagcttcat
    21481 ccttggccaa gacccatggt gcggtctcat cgatgtattt attggtacga gagatcagag
    21541 tccagactgc ttcaagcgca cgtggatagt caactgcttc catgtgtgta tggaagtctg
    21601 cgattgattg ttctgcaacc tcagcaagaa catgatcaaa ttcagtcaca ccttctacat
    21661 aggcagggat ttgtccatca aagtacttat taatcatgga aaccgtacgg ttaaggaggt
    21721 tcccaaggtc attagccaat tcatagttga tacggccgac atagtcttca ggagtaaagg
    21781 ttccgtctga accaactgga aggttacgca tgaggtagta acgaagtgga tctagtccat
    21841 aacgctctac caacatttca gggtaaacga cattcccttt tgacttagac atttttccgt
    21901 ctttcatgac aaaccaacca tgggcaatca aacgatcagg taatttaaca tccaacatca
    21961 taagaaggat tggccagtag atagagtgga agcgaaggat atcttttcct accatatgga
    22021 agactgttcc attccagaac ttgtcaaagt taccatgttc gtcttgagcg tagccaagag
    22081 ctgtcgcata gttaagaagg gcatcaatcc aaacgtagac aacgtgtttt ggatttgatg
    22141 ggacaggcac tccccatgta aaggttgtac gagataccgc caaatcttcc aaacctggct
    22201 cgatgaagtt gcgtagcatt tcattaaggc gaccatctgg cgtgataaat tcaggatgag
    22261 ctttgaaaaa ttcgaccaaa cggtcttggt atttgctaag gcgaagaaag tatgattctt
    22321 cagaaaccca ttcaacctca tgacctgatg gagcaatacc accagtcaca tttccagctt
    22381 catcacggaa aacttctgcc agctggcttt ctgtaaagaa ttcttcgtct gatactgaat
    22441 accaaccaga gtattcaccc aagtaaatat catcttgagc aagtaagcgt tcaaagacct
    22501 gtgcgacaac tttttcatgg tagtcatcag ttgtacggat aaatttatcg tatgagatat
    22561 ctagtaattg ccagagttct ttaactccaa ccgccattcc atcaacatag gcttgaggtg
    22621 taataccagc ttcttccgct ttctgctgga ttttctgacc atgttcatca agacctgtca
    22681 gataaaatac atcgtagccc atcaggcgtt tgtaacgtgc taggacatca catgcgatag
    22741 ttgtgtaggc agaaccgata tgaagtttcc cagatggata gtaaatcggc gttgtaatat
    22801 aaaaattttt ttcagacata atttttcctt tccaggcaaa tgaaacctgt ttttctaaca
    22861 cttcattata tcacattttt aatgaatttc gatagggaaa tccatacaaa aacaagatag
    22921 acgagtgtcc atcttgttga tctcattcat aacgaagggc ttcaattgga tcaagtttcg
    22981 atgccttgtt ggctggcaag actccaaaaa tcataccaac actagccgaa actgcaagac
    23041 taaatagggc aactgggatt gatactccaa cttctatacc ttctattaaa ccttgcagta
    23101 acaaacctgc taaggcagtt aaaccacttg caattgtcaa gccaattaag ccacctaaca
    23161 aggtcaaaat catggattca atcaaaaact gaattaaaat attggcacgt gttgcaccca
    23221 aagccttacg aagaccaatc tcacgagtgc gctctgtcac cgaaaccagc atgatgttca
    23281 tgacaccagt tcctccaaca aagagagaaa tccctgcgat ggaactaata atcgtcgtca
    23341 taaaactaaa cgattgttga atttctgcaa atacaaggga ctcatctgcc acctggtatt
    23401 ctccctgttg taagcctgca agctctgtca tttttcgtgc cagttctgga cccagagttg
    23461 gggttaaact ggtatcattc actcgaaaga caatattagc tatttcatct acattaaaat
    23521 tcgcagcaag ggagatattg gtagtaatag gcaagccacc aaacccatat atttttgatc
    23581 ttttagcctc cggactagta taaaccccaa tgacccggta actaaatcca ttgacttcta
    23641 caaccttgtt aatagcctct tgaggagatt caaataaact aatggacaat tcctcatcta
    23701 gcaaaatgac acttgcaaac tctttgaaat cttgctctct cagactacga cctgcaataa
    23761 tttcattctt aacagcgtcc atgtaagttc tgtttccacc tgtcaaatta gcattctcaa
    23821 cctttttatc ttgataggtc aagatggcat tcgttgaatt ggttacatag taactatcca
    23881 ctcccttcag tttagctgcc tcttggaccc aggattcttg cggttttggc ggttcaacag
    23941 gaacttcctc ttcctttcca gaaaccgtaa aagctgattg tttctgagta aaagacccat
    24001 ctttactttt tttaggagag aaaaagacgc taatattttt ctgagattta gtcatatctt
    24061 tattgacttg acgagatagg gaatcaccca aagccataat cacaacaact gatgaaacac
    24121 cgataataat cccaatcata gtaagcaaag aacgcatctt gtgagccatg atagatgaaa
    24181 aggcaaattt cagattctgc atcttagttt tcctcctttc ctaactgagc actgtcagac
    24241 gaaatgaccc catcccgaat gacaatctga cgtttggcat aggcagcaat ctcaggctca
    24301 tgcgttacca tgataatggt ttttccttct ttattcaaat caaccaataa ttgcataatt
    24361 tggttacctg ttttggtatc caaggctcct gtcggttcat ccgctaggat aatagaagga
    24421 ttgtttacca aggcacgcgc aatggctaca cgttgctttt gaccaccaga taattctgaa
    24481 ggtaaatggt gactacgttc tgtcaattca accttgtcta aatattcctc agccaacttg
    24541 cgacgttttg aagacgaaac tcctgcgtaa atcaagggca attctacatt ttgcagagca
    24601 ttgagcttcg atagaagaaa gaactgctga aagacaaaac cgatttgttg gttacggacc
    24661 ttagctagtt gtttttcacc aagcccagcc acttcttgac cttcaagata atattctcca
    24721 ctggttggtg tatccaacat gccaatcgta ttcatcagag tggacttacc agacccagat
    24781 ggtcccatga tggctacaaa ttcaccctca ttcacttcta gattgatatt tttgagaacc
    24841 tgcagttctt ggtcaccatt acggtaactt ctgaagatat tttttagact aattagttgc
    24901 ttcatcagcc ttcacctctt ttccttcttc caaggaagat gttggattac tgatgacctt
    24961 agcaccgttc gttaaaccag aagtgatttc ttgattttct gcgtcagcat ttcccaatga
    25021 aacctcaact tttttagcct tttgttgttc atccacaatc cagacataat ttttactatc
    25081 atccattact agactgctaa caggaacaag aatagcctta gttttgcttt taacctcaat
    25141 gttgacagaa aaaccttgtt tcaaatcacc aacctcgcct gtcacatcaa tagtataagg
    25201 gtatttagaa cctgtattat tcccggctgc tggactagct gcttcaccat tgtttttagg
    25261 atagtcagaa atatagctta atttcccagt ccatttttta tcaggataca ctttagaagt
    25321 aaagcttact tcttgaccta cagaaaggtt ggctagattg tactcagaca attctccctt
    25381 gacttgtaaa ttttcattgc tgacaatatg aaccataact tgactcgccc ctgttggaga
    25441 tttagaaaca ttgctattga cttcgactac agttccctct agggtactga gaacagttgt
    25501 tgcatccaat tgactttgag ccttgcttaa ttgcgctgca gcatctgcac gcgcatcacg
    25561 ggcatcaccc aattgagcat caatagaagc aacagaattt ccagccactg gagttgggct
    25621 ttgcaccgtt gcatcttctc ctcctactgg cgctggtaac tgtggagcct gagctgaagc
    25681 ggcttcattt cgtgcttgat tgagttcatt gatatgacga tctgccttag ctactgctcg
    25741 actagctgaa tcataggccg cctgcgcttc tgaactactg tacttgacta aagcctgccc
    25801 ttcgctgacc ttatcgccca cagaaacaag gatttcatct aaatcaccct tactagcatc
    25861 aaaataaaca tattgttcat tttttgctgt tactgtccct gacaataaaa cagaggatgc
    25921 cacgcttcct tcattggcaa caacaagatg agtaggctca tcttttagag cagtctgaga
    25981 aggttgtcta aagagtaaaa tccccccggc acccaataca actacactcg cagcaccgat
    26041 tgctgcatac agttgccact ttttagcttt accattcttt ttcttcataa tgaaactcct
    26101 tttctttttt acaatacttt gctattatac caaatttccc tccagcaaac aatgcagttc
    26161 agatggaaat aatggcagtt caggattaaa caatcgttcg gaattttgct tttctattgt
    26221 actagatata taatacacta tcttgactta tttggcaagc ttttttgcaa ttttttgtgc
    26281 gtagcagatt tttgcaatca aatcaaaaaa agaataaaat ataactatca aaaaagacac
    26341 tctactattt aaaagattac aaaggagttt tcaatgagag aatatgatat cattgctatc
    26401 ggtggaggta gcggaggaat cgctaccatg aaccgtgctg gtgaacatgg agccaaagca
    26461 gccgttattg aggaaaagaa attaggtgga acctgtgtca acgtcggttg tgttcctaaa
    26521 aaaatcatgt ggtacggggc gcaaatcgct gagactttcc atcaatttgg agaagactac
    26581 ggctttaaga ctactgatct taactttaac tttgcaaccc tacgtcgcaa tcgtgaagcc
    26641 tacattgatc gcgctcgttc ttcttatgat ggtagtttta aacgcaacgg tgtagacttg
    26701 attgaaggtc atgctgaatt tgtagattct catactgtaa gcgtaaatgg tgaactgatt
    26761 cgtgctaaac atatcgtgat tgctacaggt gcccatccaa gtattcctaa tattcctggt
    26821 gctgagctag gtggctcttc tgatgatgta tttgcctggg aagaacttcc agagtcagtt
    26881 gccattctag gcgctggtta tatcgccgtt gaattagctg gcgtactcca cacttttggt
    26941 gtcaagacag atctctttgt tcgccgcgat cgtcctttac gtggttttga ttcctacatc
    27001 gttgaaggtt tggtcaagga aatggaaaga acaaacttac cacttcacac tcacaaagtc
    27061 cctgtcaagt tagaaaaaac tactgacggc attaccattc atttcgaaga tggtactagt
    27121 cacacagcta gccaagttat ctgggctaca ggccgccgtc caaacgttaa gggcttgcaa
    27181 cttgaaaaag ctggagtgac tctgaacgaa cgtggcttta tccaagtgga tgaataccaa
    27241 aatactgttg ttgagggaat ctatgctcta ggtgatgtaa cgggcgagaa agaactgact
    27301 ccagttgcaa tcaaggccgg acgtacccta tctgaacgtc tctttaacgg aaaaactact
    27361 gcaaaaatgg attactcaac tattccaact gttgtctttt cacaccctgc tatcggaact
    27421 gttggtttga cagaagagca agctattaaa gaatacggtc aagaccaaat caaggtttac
    27481 aaatcaagct ttgcatctat gtactctgct tgcactcgca accgtcaaga atcccgtttc
    27541 aaactcataa cagctggttc agaagaaaaa gttgtcggac ttcatggaat tggctacggc
    27601 gttgatgaaa tgattcaggg atttgccgtt gctatcaaaa tgggagcaac caaggctgac
    27661 tttgacgcaa ctgtagcgat tcacccaact tcatctgaag aatttgtaac catgcgttaa
    27721 tcgaaacaaa agaagctgat ataaatatgt ctgcttcttt tttaatgatt ttcgaatcaa
    27781 caaaaaatta tccaacttga ctagtcacga aaatcggctg ttacagtttt tgttaccttg
    27841 tttttaaaaa atggttgaca tcgactccta tatgagtata atagttacta tacattagaa
    27901 aagaggttaa ctattttgaa aaaagctcac gtttatgcta tccctgctat tggggctgct
    27961 ctcattgctg ttttggcaca aatcagtctt ccaattggac ctgttccctt cactctgcaa
    28021 aactttgcaa tcggcttgat tgctactgtc tttagaccga gagaggctgt actttctgct
    28081 ggactctatc ttcttctagg tgctatcggt cttcctgtct ttgcaggagg tggagctggt
    28141 tttcaggctt tagttggccc tactgcaggc tatctttggt tttatctcgt ttactctgga
    28201 cttacttcct ctctaaccaa cagcaagagt ggtgttgtta agatttttct tgcaaacctc
    28261 ttgggtgatg cccttgtctt tgtcggcggg attctcagct tgcatttcct agctggaatg
    28321 gcatttgaaa aagctcttgt tgtgggggtt cttcccttta tcattccaga ccttggcaaa
    28381 cttctggcta ttagttttat tagccgtccc ctacttcaac gccttaaaaa tcagacttac
    28441 tttactaact aaaaaaggat accgagttat catgactcaa tatccttttt tttattttga
    28501 aaacttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gctgctcaaa
    28561 acactgtttt gaggttgtag atgaaactga cgaagtcagt aaccatacat acggcaaggc
    28621 aaagctgatg cggtttgaag aaattttcga agagtattag ttgcctttaa aggcatccac
    28681 catcgtttga aattcttcat ttgagagagt aatccctttg cccattttag tatggtctgg
    28741 actccaagca cgaatatcaa actttgcagg ggcaccatta aagctcacac ggttaatttc
    28801 cttggtccaa cctttttcgt tttcagaaag agtcaacaag tgctcttcga tttcaaatgt
    28861 aaattctgcc attttcttct ccttttttag tttcattagt ttattcgtaa aatcttgtag
    28921 attttaggaa aattttatat aatattgata taaaagaagg gaggccaata tgagacataa
    28981 attccagcaa gttctagata aaatacatga ttttttaaat ggacatgacc aacctgacca
    29041 gactgaaacc aactccctta cagccactat tgaagaggct atccagaaac aaaccgctgt
    29101 tcaccttatc ttgtctgaga caagctttac aggtgatatc atcaaatatg atcagcaagg
    29161 ccagcaaatt atcgtgaaaa atttttccaa aaatgtgagc cggattatcc gtataagcga
    29221 tattcaacgc ctgcgatttg tcccctcaac tgtccaaaca gcccaaaaaa atagatttaa
    29281 gaaagagtga gatgtagttg cttcatccca ctctttttct tagcgaattt gttcaaaatg
    29341 taaatgaact gcgatatgat ctccataacc acttctttcc aagtcacgtt gtaaacgata
    29401 ggaaatatag tgttctgcaa tggtaatgta acctgcgccc aataaacgat gttcaaccat
    29461 agattgaatc atactgatag tcgcacgttc caccttggct tcttgtaaat ccaaaactac
    29521 cttcttagtg acttgagcaa gattttgacg caaatcatct gtcaaaacat aaacagtttg
    29581 ggctgccttc aagatggctt ggtaaatctt atctggatta aattcagcaa tttcgccatt
    29641 acgtttgatt acttgcatag gtttctcctt tattctttgt tttctttgat ttctgccagc
    29701 attttttctt cttctactgt cagttgataa tgttcaagta aatccggtct gcgctcgtag
    29761 gttttcttta aactctcgta caatcgccac tgacgaatct tttcatgatg gccactcatc
    29821 aatacatctg gcacgaccat gcctcgataa tcatagggac gtgtgtactg aggatattct
    29881 aaaagacctg aagaaaaact atcatcttgg tggctagact ccttgccaat cacttctgga
    29941 atcaggcgaa ctgtagcatc aatcatggtc atagctgcca attctccacc agtgaggaca
    30001 tagtcgccca gggaaatctc atctgttacc aaggtcttaa tgcgctcatc ataaccctca
    30061 tagtgcccac agataaagat tagctcttcc tcttgagcca aatcttcagc ataagcctga
    30121 tcaaactgct ttccagcagg atcgaggaga ataacgcgcg gatttttctt ttcaatagca
    30181 tcaaaggaat cgaaaatagg ttgtgctctg agcaacatgc cctgaccgcc tccgtagggc
    30241 tcatcatcta catgacgggc cttttcagca ttttctcgaa aattatgata ctggatatcc
    30301 aagagccctt tttctcgagc ctttccaacg attgagtgct ccagtggaga aaacatctct
    30361 ggaaagaggg ttaaaatatc aatcttcatc gtctaaccct tctaagattt ccacatcgac
    30421 ccgtttattt ggaatatcaa cattgagaac cactggtggg atataaggta aaagcaaatc
    30481 acgtttgcct tttcgtttga ccacccagac atcattagca cctggttgca ggatttcctt
    30541 gatggttcca accaagctat caccctcata gacttccaaa ccgataatct cgtgatagta
    30601 aaattcacca tcgtctaggt cattcaaatc ttcctcagcg accttgagac tgtatccctt
    30661 gtacttttcg atagtattga tatggtacat atctttgaat ttaataatgt caaagttctt
    30721 ctgtttacgg tggctagcga tggtcactgt ttggacaaac tgatcttttt catcaaacaa
    30781 agccagctca gctccttttt taaaccgttc ttctgcaaaa tccgtcacag acaagactcg
    30841 catctcaccc tgtaatccct gcgtattaac gattttccca acattaaagt agttcatctt
    30901 gtctcctgta atctcctttt ttccatctta ttctgacaat tctcgaataa tagccgcaat
    30961 tttttccgat tctgaccatt gtaaataatg gtgattccct cctaaaatga gtttagtatt
    31021 ggaagtccaa tattctgatt ctctgtactc tttttctcta taagtctgac aaaaaataaa
    31081 tatagggaca tgagattcta tcgacaaatt ctcaaaatct tcctcagtaa tctctccaga
    31141 tatctgaaat tctggatctt gattttccaa ctctaagcct ttttcttgca ttaattccca
    31201 gattttttta ttcgtttcag gactaaatgt tgcttgagtt aagttcttaa aataaagttc
    31261 aggaccacac tcgtcaatca gcctcatctg ctcttccatt tctggataag gattttctga
    31321 aaaatcagca aacatgactt ttttagttgt cggttcaatt gctactaaag tctgacactt
    31381 aattggtttc tcgagtaatt tgcaagctaa aattccactc caactatgta cacaaagtat
    31441 atattcagaa atccctaatt cttcaagtgc ttcataaacc acatctgcaa gattatctag
    31501 attttttcca gcttggtcat aaatcggact cctacctgtg ttcggaaaat caattgtcaa
    31561 ataaccaatt gtaggaggag gtttttcaag tataagtgaa aaattttcat aacttggtag
    31621 caaacctgct ccatttagac aaactagcac tttcttttgc ttttgataag caacagagag
    31681 actaccaatt tctgtagata cttcaaacct cttcataaag aaatccactg attctatata
    31741 atgaattatt aaaaatcctt atcctttatt ttatcacgtt ccaaggattt tctcaagttg
    31801 gaggaagggg acaacatctc tactttccct tcaagaatcc ttccaaatca tgtttatgtt
    31861 ggtaattaac ggctgcggtt ttgtctttct caaagatggt tctagttagg tcaatatgct
    31921 tgatagcagc aatagcgacg gtgtagagaa tgatatcagc cagttctctg gcaagttcct
    31981 cgttcgaatc ctatcccttc ttttcgacca gagcgcctat tcaaaacctc ggctacttct
    32041 ccgacttcct ccactaactt cataaagaga ccttcatcag tccgagactg ctgttaatgt
    32101 tcgagtaagt agtcttggaa ttgcctaaac gttcaatctt ttatagtata ttgaaactag
    32161 aatagtacac ctttacttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt
    32221 tgtcctgtta ttatttcatt ttactatatc ttctattcca cacaaaaaag cgagacatcc
    32281 gtcccgccct tcttattttt cgtcaataac gattcttact tttttgtatt cagttgggac
    32341 agagtagaca atcgttctta tcgcagaaat agtgcgaccc ttacgaccga ttacacgacc
    32401 cacatcgctt tgatcaagat tcaaatgata ttccaaaaat tctggtgtat cctcaatctt
    32461 gatagttaag gcatctggtt gtgaaattaa gggtttcaca atcgcaataa tgagattttc
    32521 aatcgtatcc atctgtcaac ctactttaaa cttattttga aaatttagaa tcgtggaatt
    32581 ttttcaatac gccttctttt gaaaggatgt tacgtactgt gtctgaaggt tgagctccat
    32641 tagccaacca tgtaagaacg cggtcttctt tcaaagttac ttggttttca gcaacaagtg
    32701 ggttgtaagt tccaactgtt tcgatgaaac gtccgtcacg tggtgaacgt gaatctgcta
    32761 cgttgatacg gtagaaaggt tttttcttag aacccatacg agtcaaacgg attttaactg
    32821 ccatttttaa agtctcattt ctttaatttt ttatttcggt gaaatagctg agctatttag
    32881 cacatgttct attatagcag atttctggca ggtgtcaaga aaaaaacttg acagactata
    32941 aatttcttaa agcatttaaa aatttgttag aataggaaat aaatgtttga aagatgctat
    33001 ccgtgaatac tattttagaa aaattttata aagaacacca agtcaaaccc ttcatatcgc
    33061 ctgaacgaga tttggatact tggcttctaa gtcccaagcc cgttcccaag cgaaatatgg
    33121 acttattagt agatgattca ctagcaggag atatcatttt actatggaga atccagtttg
    33181 gtacttttac cactgaaaca tgattttaaa aactcccatt cgaatatgat tcaaaattcg
    33241 cttagaatca atgttttccc actttttcaa aaaataaatt ttcatctaat ttaaataact
    33301 ttcaattaag tgtgaatttt gcttaaaatc attaaaaaac accccatggt gtgaaccatg
    33361 aagtgttgta aatgctgtgt tgtagctagt tcttagcgtt tagagaactg gctagcttta
    33421 cgagctttct taagatctgg tttccaaaat actttgagta cacttatggt attgatgccc
    33481 ctaaacatct aaaaactctg gtagaaaaag ggtatgctct tgtcgaaact tcttttgact
    33541 cacttgacca tctaaataat accatgaaaa agaatatcct gaagaagaag ggagttactg
    33601 gactttctaa aatgaaatct tgcgacctgg atcaagccct tcatgagtat ttttcagaag
    33661 aagaattagc tggtcacttt catgtccttc tatggacttt tttacaatgg cattgctatc
    33721 acacccaata cctatctaag cgcctggttc gtaaacttta ttgcagctct tcctctaaat
    33781 ttcctaattg ttgaaccaat tgcccgtttt atactaagtt cttttcagaa accatttact
    33841 ggggaagaag ttgaagaagt tgaagatttt taagatgatg atgaaatccc aactattatc
    33901 taagccagtt ctgtaaacta ctaatatttg aaatccactt ccttttaggg tacaatggtt
    33961 ataaatgaat ttttgagagg aacagaatga aaaaactagc aacccttctt ttactgtcta
    34021 ctgtagccct agctgggtgt agcagcgtcc aacgcagtct gcgtggtgat gattatgttg
    34081 attccagtct tgctgctgaa gaaagttcca aagtagctgc ccaatctgcc aaggagttaa
    34141 acgatgcttt aacaaacgaa aacgccaatt tcccacaact atctaaggaa gttgctgaag
    34201 atgaagccga agtgattctc cacacaagcc aaggtgatat tcgcattaaa ctcttcccta
    34261 aactcgctcc tctagcggtt gaaaatttcc tcactcacgc caaagaaggc tactataacg
    34321 gtattacctt ccaccgtgtc atcgatggct ttatggtcca aactggagat ccaaaagggg
    34381 acggtacagg tggtcagtcc atctggcatg acaaggataa gactaaagac aaaggaactg
    34441 gtttcaagaa cgagattact ccttatttgt ataacatccg cggtgctctt gctatggcta
    34501 atactggtca accaaacacc aatggcagcc agttcttcat caaccaaaac tctacagata
    34561 cctcttctaa actccctaca agcaagtatc cacagaaaat tatcgaagcc tacaaagaag
    34621 gtggaaaccc tagtctagat ggcaaacatc cagtctttgg tcaagtgatt ggcggtatgg
    34681 atgttgtgga taagattgct aaagccgaaa aagatgaaaa agacaagcca actactgcta
    34741 tcacaatcga cagcatcgaa gtggtgaaag actacgattt taaatcttaa aaaccaaaag
    34801 aaatacagta tccacattcg gtactgtatt tcttttactc tcattcttaa gttaaattat
    34861 taaaatccca tatttggtct atccaacctt cataaaagtc tggatcgtgg cagaccataa
    34921 ggatagatcc cctgtattct ttgagagcgc gtttgagctc atcctttgca tccacatcca
    34981 aatggttggt cggctcgtcc agcactaaaa cgttgttttc acgattcatc aagagacaga
    35041 aacgaacctt ggcttgctct ccccctgata atacttgaat ctggctttca atatgtttgg
    35101 ttgtcaaacc acaacgggca agggctgcac ggacttctgc ttgattaagg gcaggaaagg
    35161 cattccagac agcttcaaga ggagtttggc gattaccgcc ttctacttcc tgctcaaaat
    35221 aaccaagttc taaataatct ccacgctcca cttccccagc gattggcgag ataatgccca
    35281 agagactctt caagagagtt gtttttccaa taccattagc accaataatc gcaacctttt
    35341 gattgcgttc gaaggtaaga tttaaaggct tagtaagagg acggtcgtaa ccaatttgca
    35401 agttcttggc ttggaagata aagcgccctg gtgtacgagc tggtttgaaa tcaaaggatg
    35461 gttttggttt ctcactttgg agttcgataa tatccatctt atccaatttc ttttgacgag
    35521 acatagccat attacgagtt gcaacacggg ctttattacg agccacaaag tccttgaggt
    35581 ctgcaatctc tttctgctgg cgttcgtagg ctgcctctag ctgagatttc ttcatagcat
    35641 aaacttcttg gaactggtag tagtcaccag agtaacgcgt cagctgttga ttttccacat
    35701 gatagacaat attaataacg tcattgagga atggaatatc gtgcgaaatg agaacaaagg
    35761 cattctcata gttttggaga tagcgcttga gccaatcaat atgctcagca tccaagtagt
    35821 tggtcggctc gtccaacagc aaaatatcag gcttttcaag gagaagtttt gccaaaagca
    35881 ccttggttct ttgcccacct gacaaagaag ttacatccgt atccatgcca aagtccataa
    35941 taccaagagc acgcgctact tcgtcaatct tagcatccaa ggtatagaaa tcacgactct
    36001 ccagacggtc ttgaagttct cctacttctt ccatgagagc atcaacatcc gcgccgtctt
    36061 cagccatttt catatagagg tcattgatac gagcttcagc tttgaaaagc tcatcaaaag
    36121 ccgtacggag aacatcacgc accgactgtc tttcagcaag gacagagtgc tgatccaagt
    36181 aaccagccgt cacatatttg gaccactcaa cctttccttc atctggcagc attttaccag
    36241 tcacgatact cataaaggtt gattttcctt caccattggc accgaccagg ccgatatgtt
    36301 ctcccttgag gagacggaag gacacatctt caaaaattgc acggtcacca aaaccgtgac
    36361 tcagattttt aacttctaaa atactcattt taattcctta ccttgttttt atgtaatcgt
    36421 ttataaagga gccaagccag atagccaccc aaagtgttgg tccacaaatc atcaatctca
    36481 aagacgcgat tgaaatcaaa gaaaaagtcc aagattaatt gcgtacactc gattccaaga
    36541 ctcacaagaa aactaaaaag aaggaccttt tttgttttcc gcaaatttgg aaatagataa
    36601 aggagttgga aaatcagagg aaaaaacaag aagacattga ggatattttg taaaaaaatc
    36661 caacataatt gtccaatgtc actcacttcg cccagtttcc agagagaatt gaaaggagtc
    36721 aaaagaaaaa ccaggcgtcc aagatgctga atacctggag ttcccactcc cacggtagat
    36781 tgttcttgag gagtaaagca aaaacagaca atgcaaatgc tatagaaaat gactccccag
    36841 accaaaatat gattataagt cttcttcacc attaaggatt taccgctgcg actgccttct
    36901 ggcggtcacg tttcattgtg ttagagcgca attgtccaca agctgcgtca atatctgtac
    36961 catgctcttg acgaaccaca cagttgaccc cttttttctt aagcgtatca tagaaagcca
    37021 acacgcgctc tttgggacta cggctatatt ggtcatgctc actaactggg ttataaggaa
    37081 tcaagtttac ataagacaat ttcttgatgt tcttgagcaa ttcagtcaat tccaaggctt
    37141 gttctacacc gtcgttgact tcattaagca tgatatattc aaaggttaca cgacggtttg
    37201 ttgtctcaat gtagtattca atagcagcaa agagtttttc aatcggaaag gcacggttaa
    37261 tcttcatgat acttgaacga agttcattgt taggtgcgtg aagagacacg gcaagattga
    37321 cctgaacccc ttcatcagca aagtcacgaa ttttatgagc caaacctgag gttgaaaccg
    37381 tgatgtgacg agcaccgata gccattcctt tatcatcatt gatagtacga aagaaattca
    37441 agacattgtt gtaattatca aagggctcac cgattcccat gacaacgata tggctgacgc
    37501 gttcatcctg accacgctca tcaaagtatt tctgaaccag catgatttgc gctacgattt
    37561 caccgttatt gaggtcacgt tgcttcttaa tcaaaccaga ggaacagaag gtacaaccga
    37621 tattacagcc gacctgagtg gtcacacaga cagataaacc atagtgttga cacatgagta
    37681 cagtctcaat taacataccg tcaggcaatt caaagagata tttgactgta ccatcagcag
    37741 actcttgcac aatacgttgt ttcaagggat tgaccacaaa ctggtcattg agcttagcaa
    37801 tcaaatcctt ggaaaggttg gtcatttctt caaatgactg cacacgttta cggtagagcc
    37861 attcccagat ttgatctgca cggaatttct tttctccctg ctccaatacc cattcctgca
    37921 tggtttgatg tgccaaacta tgaattgagg gtttcatttc ttctccttat tctctactca
    37981 cttctgacga atgacaaaat gacgttgttc cttgtcctct ttctgagaat gctggtcttt
    38041 ctgacgacgt ctatttttct tatctgcatt cgactttcgt ttagtttgag tcggtttctt
    38101 tccttttcta gaaggtgttt cttcttccgt cttacgcatt ttcttgtcaa atgatgctcg
    38161 cttaggggct tcattttcta agacaaaata ggcacaacca taactacaat actctaaaag
    38221 gtagtcttgt aaacgactga ttttttcaag tttttcttct gttcggtcat ccttgtaaaa
    38281 acctcgtagg cgaagctgtt cgttgctcca gtcccccacg atataatcaa acttggttaa
    38341 tacttctgaa aaacgctgat taaaagtcgt cacatcaaag gcatccttga tattttcaac
    38401 caaggaaaaa gctatccctt ccgtttcgac cttgtccccg tgtaaatgga actccggacc
    38461 aggaaacttg ttatagttgt ataattcagg tgcaatttct tttcgcatag atatcctttt
    38521 tccacgatta cttaatactt tattctacca taatttctag cagttagcac gtttctcata
    38581 aaaatgaaaa aagtctgacg attttgtcag accagaatct tataacctaa aaaagagaag
    38641 aacaattctt ccctccaact atcattattt agcagctgcg tacaattcat ctactttatt
    38701 ccagttgatt actgaaaaga aagctttgat atagtcagga cgcacgttgc ggtatttcac
    38761 gtagtaagca tgttcccaaa cgtccaagcc caagattggt tttttacctt ctgagattgg
    38821 tgtgtcttgg tttgctgttg aagtcacttc aagtttccct tctttgttga caaccaacca
    38881 tgcccaacct gaaccaaaac gagttgttgc tgctgcagtg aaggctgctt ggaattcttc
    38941 aaatgaacca aatgttgcat cgattgctgc tgccagttct gctgaaggag ctgttttctc
    39001 gggagtcatc aattcccaga aaagagcgtg gttcaagtgt ccgccaccat tgttgataag
    39061 tgcttgacgg atatcagctg gaatagattc tacatcagca agcaaggctt caaggtcttc
    39121 accgatttca gggtgttttt ctaaagctgc attggcattg ttgacataag tttgatggtg
    39181 tttgtcatgg tgcaaatgca ttgtttccgc atcgatgtat ggttccaaag cgtcgtatgc
    39241 atatggaagt tctggtaaga taatagccat ctgtaatacc tctttttctt tctatatgaa
    39301 aatgataacg caaacattca cttttttcaa gttttttgct tatagataag gaaatcactg
    39361 acatactttc taataaaata agcaaatcag caagaaaccc acgacaagat aatcctgacg
    39421 cgggttgtag tttcaacaaa tgtcaattga cctgactagc aatctgtaag agtgcctttt
    39481 caaaaaggaa acctttttca taaagacctg tcttaatctg atagtctgtc tcaatcaaat
    39541 aggaaatagc ttgcttcaaa aagctcaaag aaagtcctct cgaatctctt aatgcaaact
    39601 tgatttgata aggatttggg ttacgtccca gataactacc taaactactt gcaatctgcg
    39661 attctgtttg gccagactcc gccaaaatct tcacctgagt aaaagtccga aattgtccca
    39721 gcatgactgc aatcagcttg atttcatctt ccccttgcaa ggtcaagtct ctcaccaaat
    39781 cgcgcgcctg atccatcttt ttagttagaa taaactgagt taaatcaaaa atattgtcct
    39841 gcaaggtctt gggaattacg ttaacaatat cctcttcctc gataacagaa tccgccttat
    39901 aggactgtaa aaagagaaga tttttctgga tttcgctaaa ttgaaacccc gacttgatga
    39961 ggagattttc aaaagaatga ttggtaaact gcagaccttg tttctgactc cacttttgga
    40021 aatactggcg caattcttgt tcttttactt ctactgcatc gaagaccttg gcatcacgct
    40081 taagtaattt gactaaccgt cttttgctat ccagctttcc ttctgcaaag attatcaact
    40141 tagttgttgg agaaggattg tcaaggtatt cctcaaatga cttaagctca tcatctgtca
    40201 aaaagcgttt cttagcagtc gtgatatcca taaaataatc taatatcacg attttctcat
    40261 ccgcaaagaa aggaaggctg accaactcca attccacatc cttgtaaact acttctttca
    40321 tatcaaagta ggcaaagttg aggtcagcag aatcataccc aatctgtttc aacacttgac
    40381 tcttcatcac ttcaaactga ccctgatctg tccctgtaaa taggctcagg atcggtaaat
    40441 ttgataaagt caacttctga ctttcttcaa tggctagcat cgtctctcct ttcttctgat
    40501 ttttcgattt aatttagtca atatagcgca atttcccacg gaaatcttct aagctctcgt
    40561 agcctttttc caccatgatt gctttcagtt cattggtaat gcggtcaaaa gcactgacgc
    40621 cttctttgtg aagggtcgtt cccacctgca ccatacttgc tccacagagg atgtgttcaa
    40681 aggcatctcg accagtcaga acgccacctg ttccgataat ttggatttga ggatttaaac
    40741 gttgataaaa ggcgtgaaca ttggctagag cagtcggttt gatgtattct ccaccaattc
    40801 caccaaaacc attcttaggc cgaataacga cagattcgtc ttctatatag aggccgtttc
    40861 cgatagagtt aacgcagttg acaaacttga gcggatattt gttgaaaata gctgccgctt
    40921 ggtcaaagtg aacaatatca aaataaggtg gcaatttaat tccaagaggt ttggtgaagt
    40981 aagcaaacac ttctgccaaa atccggtctg ttgtctcaaa atcataggca atctgaggtt
    41041 tacctggaac atttggacag gaaagattta gctcagtcag accacgaaaa tcactctctt
    41101 ggactttttt caaaatagta tgggtttcct ctggagacat gccgaccaga gataagaaga
    41161 aagttcggtt cgactctttt tcctgcaaat ctaaaagata atccaaataa tagtctaagc
    41221 cattatttgg caagcccata gagttgatgg aaccaagtgg aacatcttgg tagcgtggct
    41281 caggattccc ctgacggaag tccaaggtcg ctgtcttagt aacaaaggtt cctgccgctg
    41341 agtttttgac ctcttctaac tcctctatcg tcatacaagc cacacctgct gcattcatca
    41401 agcaattgtc aaactcaaaa ccagcaattt gtgttttcgt tgataccatg attctaatcc
    41461 tccagatttc ttttattgct aatattatac cataatttta attctaggtt tactagattc
    41521 tgtggttcta tctatttatt cggaaaagaa agtctagtac agaagtcatg ctatggaaac
    41581 agctagtgta ttggaagttt gaacaaggct tgggtaacag acttttgaat gaagtccaag
    41641 caacaaggag aaatctcaac catgagtcca ttccatgact ctgttgctct agaaaacctt
    41701 gcaatagtca agtgataacg aataaaactt tggattgaaa tatccccatg attatctatt
    41761 acgaggagaa atctcaacct ttatcatttt
//
LOCUS       DAVKWS010000012        38639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKWS010000012 DAVKWS010000000
VERSION     DAVKWS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.012"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            371..598
                     /locus_tag="VW852_000689"
     CDS             371..598
                     /locus_tag="VW852_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271866.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            886..3798
                     /gene="nanA"
                     /locus_tag="VW852_000690"
     CDS             886..3798
                     /gene="nanA"
                     /locus_tag="VW852_000690"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV0271867.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKENEYFTMSVLDNTALIEGRGANGEQFYDKYTDAPLKVRPGQWNSVTFTVEQPTAE
                     LPHGRVRLYVNGVLSRTSLKSGNFIKDMPDVNQAQLGATKRGNKTVWGSNLQVRNLTV
                     YDRALSPEEVQTRSQLFERGDLEKKLPEGAKISEKEDVFEGGMNNKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KEVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKERTIREEF"
     gene            4799..5251
                     /locus_tag="VW852_000691"
     CDS             4799..5251
                     /locus_tag="VW852_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0271868.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPKNVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            5270..6607
                     /locus_tag="VW852_000692"
     CDS             5270..6607
                     /locus_tag="VW852_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV0271869.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            6686..7570
                     /locus_tag="VW852_000693"
     CDS             6686..7570
                     /locus_tag="VW852_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0271870.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            7570..8403
                     /locus_tag="VW852_000694"
     CDS             7570..8403
                     /locus_tag="VW852_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0271871.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            8421..10514
                     /gene="nanB"
                     /locus_tag="VW852_000695"
     CDS             8421..10514
                     /gene="nanB"
                     /locus_tag="VW852_000695"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV0271872.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            10526..11629
                     /locus_tag="VW852_000696"
     CDS             10526..11629
                     /locus_tag="VW852_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0271873.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDVCKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            11803..12501
                     /locus_tag="VW852_000697"
     CDS             11803..12501
                     /locus_tag="VW852_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0271874.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            12520..14037
                     /locus_tag="VW852_000698"
     CDS             12520..14037
                     /locus_tag="VW852_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0271875.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            14162..15490
                     /locus_tag="VW852_000699"
     CDS             14162..15490
                     /locus_tag="VW852_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0271876.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            15743..16609
                     /locus_tag="VW852_000700"
     CDS             15743..16609
                     /locus_tag="VW852_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0271877.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            16625..17464
                     /locus_tag="VW852_000701"
     CDS             16625..17464
                     /locus_tag="VW852_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0271878.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            17737..18189
                     /locus_tag="VW852_000702"
     CDS             17737..18189
                     /locus_tag="VW852_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0271879.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            18331..18639
                     /locus_tag="VW852_000703"
     CDS             18331..18639
                     /locus_tag="VW852_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271880.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            18925..19569
                     /locus_tag="VW852_000704"
     CDS             18925..19569
                     /locus_tag="VW852_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEV0271881.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIEVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            19738..20655
                     /locus_tag="VW852_000705"
     CDS             19738..20655
                     /locus_tag="VW852_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV0271882.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            20673..21557
                     /locus_tag="VW852_000706"
     CDS             20673..21557
                     /locus_tag="VW852_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0271883.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(21573..22424)
                     /locus_tag="VW852_000707"
     CDS             complement(21573..22424)
                     /locus_tag="VW852_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV0271884.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            22624..24681
                     /gene="pbp2b"
                     /locus_tag="VW852_000708"
     CDS             22624..24681
                     /gene="pbp2b"
                     /locus_tag="VW852_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV0271885.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     AYMVQTALGLMGQTYQPNMFVGTSNLESAMGKLRSTFGEYGLGSATGIDLPDESTGFV
                     PKDYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGAAVSISGKTGTA
                     ESYVEGGQEANNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYNQHH
                     PMN"
     gene            24692..25288
                     /gene="recR"
                     /locus_tag="VW852_000709"
     CDS             24692..25288
                     /gene="recR"
                     /locus_tag="VW852_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV0271886.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMNSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            25464..26507
                     /locus_tag="VW852_000710"
     CDS             25464..26507
                     /locus_tag="VW852_000710"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV0271887.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            26591..27964
                     /locus_tag="VW852_000711"
     CDS             26591..27964
                     /locus_tag="VW852_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV0271888.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            27951..28562
                     /locus_tag="VW852_000712"
     CDS             27951..28562
                     /locus_tag="VW852_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0271889.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEGSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDINRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPVLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            28653..29357
                     /locus_tag="VW852_000713"
     CDS             28653..29357
                     /locus_tag="VW852_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV0271890.1"
                     /translation="MNLWDIFFTTQATKPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIVKEA"
     gene            29576..30949
                     /gene="ftsA"
                     /locus_tag="VW852_000714"
     CDS             29576..30949
                     /gene="ftsA"
                     /locus_tag="VW852_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV0271891.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            30966..32225
                     /gene="ftsZ"
                     /locus_tag="VW852_000715"
     CDS             30966..32225
                     /gene="ftsZ"
                     /locus_tag="VW852_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV0271892.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            32230..32901
                     /locus_tag="VW852_000716"
     CDS             32230..32901
                     /locus_tag="VW852_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV0271893.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            32911..33450
                     /locus_tag="VW852_000717"
     CDS             32911..33450
                     /locus_tag="VW852_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV0271894.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            33450..33713
                     /locus_tag="VW852_000718"
     CDS             33450..33713
                     /locus_tag="VW852_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV0271895.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            33710..34495
                     /locus_tag="VW852_000719"
     CDS             33710..34495
                     /locus_tag="VW852_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV0271896.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            34504..35292
                     /gene="divIVA"
                     /locus_tag="VW852_000720"
     CDS             34504..35292
                     /gene="divIVA"
                     /locus_tag="VW852_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV0271897.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            35640..38432
                     /locus_tag="VW852_000721"
     CDS             35640..38432
                     /locus_tag="VW852_000721"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV0271898.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPVGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRTWGVPLPIFYAEDGTAIMVAETIEHVAQLFEKHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        12031 a         6769 c         8388 g        11451 t
ORIGIN      
        1 gtcagtctta tctacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt
       61 ttgctctttg attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac
      121 tataaatgtt cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa
      181 ctaaaatcta ctatattaca ctatattgaa agcgttttaa aaataaggta taataaattt
      241 attaacactt ataaaaagtg atagaatcta tctttatgta tatttaaaga tagattgctg
      301 taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa gcttagaaaa
      361 ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa gacaagttga
      421 actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc acttatttgg
      481 gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat ttcccattat
      541 ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata aggattgagc
      601 aggaagtatg gtgtaaatag cataagctga tgtccatcat ttgcttataa agagatagtt
      661 tagtttaatt gcagcggtgt cctggtagat aaactagatt ggcaggagtc tgattggaga
      721 aaggagaggg gaaatttggc accaatttga gatagtttgt ttagttcatt tttgtcattt
      781 aaatgaactg tagtaaaaga aaattaataa aagacaaact aagtgcattt tctggaataa
      841 atgtcttatt tcagaaatcg ggatatagat atagagagga tcagtatgaa tcggagtgtt
      901 caagaacgta agtgtcgtta tagcattagg aaactatcgg taggagcggt ttctatgatt
      961 gtaggagcag tggtatttgg aacgtctcct gttttagctc aagaaggggc aagtgagcaa
     1021 cctctggcaa atgaaactca actttcgggg gagagctcaa ccctaactga tacagaaaag
     1081 agccagcctt cttcagagac tgaactttct ggcaataagc aagaacaaga aaggaaagat
     1141 aagcaagaag aaaaaattcc aagagattac tatgcacgag atttggaaaa tgtcgaaaca
     1201 gtgatagaaa aagaagatgt tgaaaccaat gcttcaaatg gtcagagagt tgatttatca
     1261 agtgaactag ataaactaaa gaaacttgaa aacgcaacag ttcacatgga gtttaagcca
     1321 gatgcatcag ctccacgctt ttacaatctc ttctctgtat ccagtgacac caaggaaaat
     1381 gagtacttta ctatgtcggt ccttgataat acagctctta ttgaagggcg tggcgctaac
     1441 ggagagcagt tctatgataa gtatacagat gctcctttga aagttcgccc aggacaatgg
     1501 aattccgtga cctttactgt cgaacaaccg acagcggagt tacctcatgg acgagttcgt
     1561 ctctatgtga acggtgtctt atctcgaaca agtctcaaat ctggtaactt tatcaaagat
     1621 atgccagatg ttaatcaagc tcaacttgga gcaaccaaac ggggcaataa gacagtttgg
     1681 ggaagcaatc tacaagttcg aaacctaacc gtctatgatc gtgccttaag cccagaagaa
     1741 gttcagacga gaagccaact atttgaaaga ggtgatttgg agaagaaact tccagaaggt
     1801 gcgaaaatct cagagaaaga agacgtcttt gaagggggaa tgaacaacaa accaaataaa
     1861 gatggaatta agagttatcg tattccagct cttctcaaga cagataaagg aactctgatt
     1921 gcaggtgcag atgaacgccg tcttcattct agtgactggg gtgatatcgg tatggtcatc
     1981 agacgtagtg aagataatgg taaaacttgg ggtgaccgag taaccattac caacttacgt
     2041 gacaatccaa aagcttctga cccatcgatc ggttcaccag tgaatatcga tatggtgttg
     2101 gttcaagatc ctgaaaccaa acgaatcttt tctatctatg acatgttccc agaagggaag
     2161 ggaatctttg gaatgtcttc acaaaaagaa gaagcctaca aaaaaatcga tggaaaaacc
     2221 tatcaaatcc tctatcgtga aggagaaaag ggagcttata ccattcgaga aaatggtact
     2281 gtctatacac cagatggtaa ggcgacagac tatcgcgttg ttgtagatcc tgttaaacca
     2341 gcctatagcg acaagggtga tctatacaag ggtgaccaat tactaggcaa tatctacttc
     2401 acaacaaaca aaacttctcc atttagaatt gccaaggata gctatctatg gatgtcctac
     2461 agtgatgacg acgggaagac atggtcagcg cctcaagata ttactccgat ggtcaaagcc
     2521 gattggatga aattcttggg tgtaggtcct ggaacaggaa ttgtacttcg gaatgggcct
     2581 cacaagggac ggattttgat accggtttat acgactaata atgtatctca cttaaatggc
     2641 tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa aaacttggca tgctggagaa
     2701 gcggtcaacg ataaccgtca ggtagacggt caaaagatcc actcttctac gatgaacaat
     2761 agacgtgcgc aaaatacaga atcaacggtg gtacaactaa acaatggaga tgttaaactc
     2821 tttatgcgtg gtttgactgg agatcttcag gttgctacaa gtaaagacgg aggagtgact
     2881 tgggagaagg atatcaaacg ttatccacag gttaaagatg tctatgttca aatgtctgct
     2941 atccatacga tgcataatgg aaaagaatac atcatcctca gcaatgcagg tggaccgaaa
     3001 cgtgaaaatg ggatggtcca cttggctcgt gtcgaagaaa atggtgagtt gacttggctc
     3061 aaacacaatc caattcaaaa aggagagttt gcctataatt cgctccaaga attaggaaat
     3121 ggggagtatg gcatcttgta tgaacatact gaaaaaggac aaaatgccta taccctatca
     3181 tttagaaaat ttaattggga atttttgagc aaaaatctga tttctcctac cgaagcgaaa
     3241 gtgaagcgaa ctagagagat gggcaaagga gagatgggca aagaagttat tggcttggag
     3301 ttcgactcag aagtattggt caacaaggct ccaacccttc aattggcaaa tggtaaaaca
     3361 gcgactttcc taacccagta tgatagcaag accttgttgt ttgcagtaga taaggaagat
     3421 atcggacagg aaattattgg tatagctaaa ggaagcatcg aaagtatgca taatcttcct
     3481 gtaaatctag caggtgccag agttcctggc ggagtaaatg gtagcaaagc agcggtgcat
     3541 gaagttccag aatttacagg gggagttaat ggtacagagc cagctgttca tgaaatcgca
     3601 gagtataagg gatctgattc gcttgtaact cttactacaa aagaagatta tacttacaaa
     3661 gctcctcttg ctcagcaggc acttcctgaa acaggaaaca aggagagtga cctcctagct
     3721 tcactaggac taacagcttt cttccttggt ctgtttacgc tagggaaaaa agagagaaca
     3781 ataagagaag aattctaaac atttgatttt gtaaaaatag aaggagatag caggttttca
     3841 agcctgctat ctttttttga tgacattcag gctgatacga aatcataaga ggtctgaaac
     3901 tactttcaga gtagtctgtt ctataaaata tagtagattg aaataagatg tgaacaactc
     3961 tatcaggaaa gtcaaattaa tttatagaaa aattttagca gtcaaggtgt actgttatag
     4021 attcaatata ttatgcgact attaaccttg tcttctccta aaattgactt tcttgttttc
     4081 ttatcttgtc cactcgaaac aagtattgta agaatttgat tatttttgaa agtactttta
     4141 atatacttga tatagttaaa aaagatttga aactaaattc caaattagaa aaagacttga
     4201 aatactaaaa aaaaaaagta tactctaatt gaaaacggta acaaaactaa tttagagaat
     4261 gaaatataga gtatttctct cttaaaagtt tttggtgaaa cgagatgtag aaaggagatt
     4321 tagccaaaga gtctattagt gctagaataa tagattagaa ttattttaga aaaacgaagt
     4381 gagcagctta taaattcaag tccccaaata gattcatact agtatctttt gcaaaaaata
     4441 aagggcgact tccttcatga atatcaattt catctataag gaaggtagct aattgaacta
     4501 acttatttat tctgtttgtc gctagaaaaa tcagacctcc ttgtgaagat tgaggagata
     4561 cttaatgaaa atcaaagaag aaactagcaa gctagtagca gattgcccaa aacaccgctt
     4621 tgaggttgta gataagactg acgtatataa tccaaggtga agcgactgtg gtttgaagag
     4681 attttcaaag agtataggct agagagtagt gtttttatgt ccttctagta gaaaatgcta
     4741 gacagaagaa tggggaactt ggatagtaaa aatagattga gaaaggaggt tagaagagat
     4801 gattattaca aaaattagcc gtttaggaac ttatgtggga gtcaatccac attttgcaac
     4861 attaatagat tttctagaaa aaacaggact agaaaattta acagaaggtt cgattgctat
     4921 cgatggtaat cgattgtttg ggaattgctt tacttatcta gcagatggtc aagcaggggc
     4981 tttctttgaa acacaccaaa aatatttgga tattcattta gttttggaaa acgaagaagc
     5041 catggctgtt acatcgccga aaaatgtaag cgttacccaa gaatatgatg aagagaaaga
     5101 tattgaatta tacacaggga aagtggaaca gttggttcat ttgagagctg gtgaatgcct
     5161 catcactttt ccagaagatt tacatcaacc caaggttcgt ataaatgatg aacctgtgaa
     5221 aaaagttgtc tttaaagttg cgatttctta atgtagaaag agaagaacga tgaaaaaaat
     5281 gagaaagttt ttatgtctag ctggaattgc gctagcggct gttgccttgg tagcttgttc
     5341 aggaaaaaaa gaagctacaa ctagtactga accaccaaca gaattatctg gtgagattac
     5401 aatgtggcac tcctttactc aaggaccccg tttagaaagt attcaaaaat cagcagatgc
     5461 tttcatgcaa aagcatccaa aaacgaaaat caagattgaa acattttctt ggaatgactt
     5521 ctatactaaa tggactacag gtttagcaaa tggaaatgtg ccagatatca gtacagctct
     5581 tcctaaccaa gtaatggaaa tggtcaactc agatgctttg gttccgctaa atgattctat
     5641 caagcgtatt ggacaagata aatttaacga aactgcctta aatgaagcaa aaatcggaga
     5701 tgattactac tctgttcctc tttattcaca tgcacaagtc atgtgggtta gaacagattt
     5761 gttaaaagaa cataatattg aggttcctaa aacttgggat caactctatg aagcttctaa
     5821 aaaattgaaa gaagctggag tttatggctt gtctgttccg tttggaacaa atgacttaat
     5881 ggcaacacgt ttcttgaact tctacgtacg tagtggcgga ggaagcctct taacaaaaga
     5941 tcttaaagca gacttgacaa gccaacttgc tcaagatggt attaaatact gggttaaatt
     6001 gtataaagaa atctcacctc aagattcttt gaactttaat gtccttcaac aagctacctt
     6061 gttctatcaa ggaaaaacag catttgactt taactctggc ttccatatcg gaggaattaa
     6121 tgccaacagt cctcaattga ttgattcgat tgatgcttat cctattccaa aaatcaaaga
     6181 gtctgataaa gaccaaggaa ttgaaacctc aaacattcca atggttgttt ggaaaaattc
     6241 aaaacatcca gaagttgcta aagcattctt agaagcactt tataatgaag aagactacgt
     6301 taaattcctt gattcaactc cagtaggtat gttgccaact attaagggga ttagcgattc
     6361 tgcagcctat aaagaaaatg aaactcgtaa gaagtttaaa catgctgaag aagtaattac
     6421 tgaagctgtt aaaaaaggta ctgctattgg ttatgaaaat ggaccaagtg tacaagctgg
     6481 tatgttgact aaccaacaca ttattgaaca aatgttccaa gatatcatta caaatggaac
     6541 agatcctatg aaagcagcaa aagaagcaga aaaacaatta aacgatttat ttgaggctgt
     6601 tcagtagatg taaaagacta gaaaataggt gggctagtga gctgaaaagc tctagcccaa
     6661 tcttgtaaaa gaagggagaa ggagaatggt taaagaacgt aatttaactc gctggatatt
     6721 tgttttgcca gctatgatta tcgtaggatt actctttgtt tatccgtttt tctcgagtat
     6781 tttttatagc tttaccaata agcatttgat tatgcctaat tataaatttg ttggtttggc
     6841 taactataaa gctgtgctat cagatcccaa cttctttaat gcgttcttta attcaattaa
     6901 gtggaccatt ttctcattag ttggtcaagt tttagtaggg tttgtattgg ctttagctct
     6961 tcacagagta cgccacttca agaaattata taggacctta ttgattgttc cttgggcatt
     7021 tcctaccatc gttattgcct tctcttggca gtggattcta aacggggttt atggctactt
     7081 acctaatcta atcgtaaaat taggtttaat ggaacataca cctgcatttt tgacagatag
     7141 tacatgggca ttcctatgtt tggtgtttat caacatttgg tttggagcac caatgattat
     7201 ggttaatgtg ctttcagctt tgcaaacagt accagaagaa caatttgagg ctgctaagat
     7261 agatggtgct tcaagttggc aggtgttcaa gtttatcgtc tttccacata ttaaagtggt
     7321 tgtaggactt ctagttgttt tgagaactgt atggatcttt aataactttg acattatcta
     7381 cctcattact ggtggtggac cagccaatgc tacaacgacg cttccaattt ttgcttacaa
     7441 cctgggctgg ggaactaaat tgttgggtcg tgcttcagca gttacagtat tgctctttat
     7501 cttcttggtg gcgatttgct ttatctactt tgctatcatc agtaagtggg aaaaggaggg
     7561 tagaaaataa tgaagaagaa atccagtatt tatttagata ttctctcaca tgtactttta
     7621 gttggtgcga ccatcgttgc aattttccca ttggtatgga ttatcatatc ttctgtcaaa
     7681 gggaaagggg aattaactca gtatccaaca cgattttggc ctgaacagtt tacattagat
     7741 tatttcactc atgttatcaa cgatttgcac ttcattgata acattcgaaa cagtttaatc
     7801 attgccttgg ctacaaccct tattgcgatt attatttctg ctatggcagc ctatggtatt
     7861 gttcgattct ttcctaaatt gggagcaatc atgtcgagac tactcgtcat tacctacatt
     7921 ttcccaccaa ttttgttagc aattccctat tcaattgcca ttgctaaagt tgggttaaca
     7981 aatagtttat ttggcttgat gatggtttat ctatctttta gtgttccata tgcagtttgg
     8041 ctcttagttg gatttttcca aacagttcca attggaattg aagaagcggc tagaattgat
     8101 ggtgcaaata aatttgttac gttttataaa gttgtgctac cgattgtagc accaggtatt
     8161 gtagcaacag ctatttatac atttatcaat gcttggaatg aattcctgta tgccttgatt
     8221 ttgattaaca atacaggaaa gatgacagta gcagtagccc ttcgttcact taatggttca
     8281 gaaatactag actggggaga tatgatggca gcgtctgtta ttgtagttct tccatcaatt
     8341 attttcttct ctatcatcca aaataagatt gcaagtggat tatcagaagg atctgtgaag
     8401 tagacgaaag aaggaaaaaa atgaataaaa gaggtcttta ttcaaaacta ggaatttctg
     8461 ttgtaggcat tagtctttta atgggagttc ccactttgat tcatgcgaat gaattaaact
     8521 atggtcaact gtccatatct cctatttttc aaggaggttc atatcaactg aacaataaga
     8581 gtatagatat cagctctttg ttattagata aattgtctgg agagagtcag acagtagtaa
     8641 tgaaatttaa agcagatgaa ccaaactctc ttcaagcttt gtttggccta tctaatagta
     8701 aagcaggctt taaaaataat tacttttcaa ttttcatgag agattctggt gagataggtg
     8761 tagaaataag agacgcccaa aagggaataa attatttatt ttctagacca gcttcattat
     8821 ggggaaagca taaaggacag gcagttgaaa atacactagt atttgtatct gattctaaag
     8881 ataaaacata cacaatgtat gttaatggaa tagaagtgtt ctctgaaaca gttgatacat
     8941 ttttgccaat ttcaaatata aatggtatag ataaggcaac actaggagct gttaatcgtg
     9001 aaggtaagga acattacctc gcaaaaggaa gtattgatga aatcagtcta tttaacaaag
     9061 caattagtga tcaggaagtt tcaaatattc ccttgtcaaa tccatttcag ttaattttcc
     9121 aatcaggaga ttctactcaa gctaactatt ttagaatacc gacactatat acattaagta
     9181 gtggaagagt tctatcaagt attgatgcac gttatggtgg gactcatgat tctaaaagta
     9241 agattaatat tgccacttct tatagtgatg ataatgggaa aacgtggagt gagccaattt
     9301 ttgctatgaa gtttaatgac tatgaggagc agttagttta ctggccacga gataataaat
     9361 taaagaatag tcaaattagt ggaagtgctt cattcataga ttcatccatt gttgaagata
     9421 aaaaatctgg gaaaacgata ttactagctg atgttatgcc tgcgggtatt ggaaataata
     9481 atgcaaataa agccgactca ggttttaaag aaataaatgg tcattattat ttaaaactaa
     9541 agaagaatgg agataacgat ttccgttata cagttagaga aaatggtgtc gtttatgatg
     9601 aaacaactaa taaacctaca aattatacta taaatgataa gtatgaagtt ttggagggag
     9661 gaaagtcttt aacagtcgaa caatattcgg ttgattttga tagtggctct ttaagagaaa
     9721 ggcataatgg aaaacaggtt cctatgaatg ttttctacaa agattcgtta tttaaagtga
     9781 ctcctactaa ttatatagca atgacaacta gtcagaatag aggagagagt tgggaacaat
     9841 ttaagttgtt gcctccgttc ttaggagaaa aacataatgg aacttacttg tgtcctggac
     9901 aaggtttagc attaaaatca agtaacagat tgatttttgc aacatatact agtggagaac
     9961 taacctatct catttctgat gatagtggtc aaacatggaa gaaatcctca gcttcaattc
    10021 cgtttaaaaa tgcaacagca gaagcacaaa tggttgaact gagagatggt gtgattagaa
    10081 cattctttag aaccactaca ggtaagatag cttatatgac tagtagagat tctggagaaa
    10141 catggtcgga agtttcgtat attgatggaa tccaacaaac ttcatatggc acacaagtat
    10201 ctgcaattaa atactctcaa ttaattgatg gaaaagaagc agtcattttg agtacaccaa
    10261 attctagaag tggccgtaag ggaggtcaat tagttgtcgg tttagtcaat aaagaagatg
    10321 atagtattga ttggaaatac cactatgata ttgatttgcc ttcgtatggt tatgcctatt
    10381 ctgcgattac agaattgcca aatcatcaca taggtgtact gtttgaaaaa tatgattcgt
    10441 ggtcgagaaa tgaattgcat ttaagcaatg tagttcagta tatagatttg gaaattaatg
    10501 atttaacaaa ataaaggaga aaaacatggt taaatacggt gttgttggag cagggtattt
    10561 tggagctgaa ttggctcgct acatgcaaaa aaatgatgga gcagagatta ctcttctcta
    10621 tgatccagat aatgcagagg cgattgcaga agaattggga gcaaaagtag caagttcctt
    10681 agatgagttg gtttctagcg atgaagtaga ctgtgttatc gtcgcaactc caaataatct
    10741 tcataaggaa ccggttatta aggctgcaca gcatggtaaa aatgttttct gtgaaaaacc
    10801 aattgcgctt tcttatcaag attgtcgcga gatggtagat gtgtgtaaag aaaacaatgt
    10861 aacctttatg gcaggacata ttatgaattt ctttaatggt gttcatcatg caaaagaact
    10921 cattaatcaa ggagttatcg gagacgttct atattgtcat acagctcgta atggttggga
    10981 agaacaacaa ccgtcagtat catggaaaaa aattcgtgaa aaatcaggtg gtcacttgta
    11041 tcaccacatc catgaattgg attgcgttca attccttatg gggggcatgc ctgaaactgt
    11101 aaccatgaca ggtggaaatg tggcccatga aggtgaacat ttcggtgatg aagatgatat
    11161 gatttttgtc aatatggaat tttctaataa gcgttttgcc ttgttagaat ggggttcagc
    11221 ttatcgttgg ggtgaacatt atgtcttaat ccaaggaagc aaaggtgcca tccgcttaga
    11281 cttattcaac tgtaaaggaa ctcttaagct agatgggcaa gaaagctatt tcttgattca
    11341 cgaatcgcaa gaagaagatg atgatcggac tcgtatctat catagtacag agatggatgg
    11401 agcaattgct tatggtaaac caggtaaacg tactccatta tggctatcat ctgtcattga
    11461 taaagaaatg cgctatctgc atgagattat ggaaggagct ccagtatcag aagaatttgc
    11521 aaaacttttg acaggtgaag ctgccctaga agcaattgct actgcagatg cttgtaccca
    11581 gtctatgttt gaagatcgca aagtaaaatt gtcagaaatt gtaaaataaa ttttggtatt
    11641 ctcctattta taggtcgact tgctcctctg aaagtacttt tagaggagct gtttgacttt
    11701 gctagttttt gaaactgaaa tctattatac tacaaactat tgaaagcgtt ttaattttaa
    11761 ggtataataa tctcatagaa ataaagaaaa ggaggaaaga ggatgccaca gattagcaaa
    11821 gaagccttga ttgagcaaat caaagatgga atcatcgttt cttgtcaggc tcttcctcat
    11881 gaaccgcttt atacagaagc gggaggggtg attcccttgc tggtcaaagc ggctgagcaa
    11941 ggtggagcag tcggtatccg agcaaacagt gttcgcgata tcaaggaaat taaggaagtc
    12001 actaaacttc caatcattgg gattatcaaa cgtgattatc cacctcagga acccttcatc
    12061 acggctacta tgaaagaagt tgatgaattg gcagaactgg acatcgaggt gattgctctg
    12121 gattgtacca agcgtgaacg ctacgatggt ttggaaattc aagagttcat tcgtcaggtt
    12181 aaggagaaat atcctaatca gcttttgatg gctgatacta gtatcttcga agaagggcta
    12241 gcagctgtag aagcaggaat tgactttgtc ggaacaacct tatcaggcta cacatcctac
    12301 agtccaaaag tagacggtcc agattttgaa ttgattaaga aactctgtga tgctggtgta
    12361 gatgtcattg cagaaggaaa aattcataca ccagaacaag ccaaacaaat ccttgaatat
    12421 ggagtgcgag gcatcgttgt tggtggcgcc attactagac caaaagagat tacagaacgc
    12481 ttcgttgcta gtcttaaata agatgtgagg gggagtttta tgtttaaagt tttacaaaaa
    12541 gttggaaaag cttttatgtt acctatagct atacttcctg cagcaggtct acttttgggg
    12601 attggtggtg cactttcaaa cccaaccacg atagcaactt atccaatact agacaatagt
    12661 atttttcaat cgatattcca agtaatgagc tctgcaggag aggttgtatt cagtaatttg
    12721 tcactacttc tctgtgtggg attatgtatt ggcttagcga aacgagataa aggaaccgct
    12781 gcgttagcag gagtaactgg ttacttagtt atgactgcaa cgatcaaagc tttggtaaaa
    12841 ctttttatgg cagaaggatc tgcaattgat actggagtta ttggagcatt agctgtcgga
    12901 atagttgccg tatatttgca caaccgatat aacaatattc aattaccttc cgctttagga
    12961 ttctttggag gttcacgctt cgttcctatt gttacatcgt tctcttctat cttgattggc
    13021 tttgtcttct ttgttatttg gccacctttc caacaacttc ttgtttctac aggtggatat
    13081 atttctcagg cgggtccaat tggaactttt ctatatggat ttttaatgag actttctgga
    13141 gcagtaggct tacatcatat aatttaccct atgttttggt atactgaact tggtggtgtt
    13201 gaaactattg caggacaaac agtggttgga gctcaaaaaa tattttttgc tcaattagcc
    13261 gatccggccc attctggatt atttacagaa ggaacaaggt tttttgcagg tcgtttctca
    13321 acaatgatgt tcggtttacc ggctgcctgt ttagcgatgt accatagtgt tcctaaaaat
    13381 cgtcgtaaaa aatacgcggg tttgtttttt ggagttgctt taacatcttt tattaccggt
    13441 attacagaac caattgaatt tatgtttcta ttcgtcagtc cggttctata tgttgttcac
    13501 gcattccttg atggtgttag cttctttatt gcagacgtct taaatatttc aataggaaac
    13561 acattttcag gaggtgtaat cgatttcact ttatttggaa ttttgcaggg gaacgctaag
    13621 acgaattggg ttcttcagat tccaattgga cttatttgga gtgttttgta ttatattatt
    13681 tttagatggt tcattactca attcaacgtt ctaacaccag gacgaggaga agaagtagat
    13741 tctaaagaaa tttctgaatc cgcagattca acttcaaata ctgcagatta tttaaaacag
    13801 gatagcctac aaattatcag agcgttgggt ggatcaaata atatagaaga tgtagatgct
    13861 tgtgtgacac gtttacgtgt agctgtaaaa gaagttaatc aagttgataa agcactttta
    13921 aaacaaattg gtgcagttga tgtcttagaa gtgaagggtg gcattcaagc aatctatgga
    13981 gcaaaagcaa tcttatataa aaatagtatt aatgaaattt taggtgtaga tgattaagta
    14041 cttactgact taataaaaaa cagaggagag tgatggatga gtaggatgaa atgaaatcgc
    14101 atacaagaaa taaagaactc attatccaag ttggatacgc ttattacata ggagaataca
    14161 aatgaaattt agaaaattag cttgtacagt acttgcgggt gctgcggttc ttggtcttgc
    14221 tgcttgtggc aattctggcg gaagtaaaga tgctgccaaa tcaggtggtg acggtgccaa
    14281 aacagaaatc acttggtggg cattcccagt atttacccaa gaaaaaactg gtgacggtgt
    14341 tggaacttat gaaaaatcaa tcatcgaagc gtttgaaaaa gcaaacccag atataaaagt
    14401 gaaattggaa accatcgact tcaagtcagg tcctgaaaaa atcacaacag ccatcgaagc
    14461 aggaacagct ccagacgtac tctttgatgc accaggacgt atcatccaat acggtaaaaa
    14521 cggtaaattg gctgagttga acgacctctt cacagatgaa tttgttaaag atgtcaacaa
    14581 tgaaaacatc gtacaagcaa gtaaagctgg agacaaggct tatatgtatc cgattagttc
    14641 tgccccattc tacatggcaa tgaacaagaa aatgttagaa gatgctggag tagcaaacct
    14701 tgtaaaagaa ggttggacaa ctgatgattt tgaaaaagta ttgaaagcac ttaaagacaa
    14761 gggttacaca ccaggttcat tgttcagttc tggtcaaggg ggagaccaag gaacacgtgc
    14821 ctttatctct aacctttata gcggttctgt aacagatgaa aaagttagca aatatacaac
    14881 tgatgatcct aaattcgtca aaggtcttga aaaagcaact agctggatta aagacaattt
    14941 gatcaataat ggttcacaat ttgacggtgg ggcagatatc caaaactttg ccaacggtca
    15001 aacatcttac acaatccttt gggcaccagc tcaaaatggt atccaagcta aacttttaga
    15061 agcaagtaag gtagaagtgg tagaagtacc attcccatca gacgaaggta agccagctct
    15121 tgaatatctt gtaaacgggt ttgcagtatt caacaataaa gacgacaaga aagtcgctgc
    15181 atctaagaaa ttcatccagt ttatcgcaga tgacaaggag tggggaccta aagacgtagt
    15241 tcgtacaggt gctttcccag tccgtacttc atttggaaaa ctttatgaag acaaacgcat
    15301 ggaaacaatc agtggctgga ctcaatacta ctcaccatac tacaacacta ttgatggatt
    15361 tgctgaaatg agaacgcttt ggttcccaat gttgcaatct gtatcaaatg gtgacgaaaa
    15421 accagcggat gctttgaaag ccttcactga aaaagcaaac gaaacaatca aaaaagctat
    15481 gaaacaatag tccttagtta ttctatgaaa aagtaatttt ttaaagaacc taagagtgta
    15541 tacccccttt tccctctaca cagatagtgt aagaaaaggg ggcttttgtt taaaatgtaa
    15601 gaaactgtca cgaaattaaa atgaagttct tacataagcg aatcataaaa aatttcattt
    15661 tgattttaaa acagttcaag aaagtcaaaa aattattcta tttgaaagag aggtgccgac
    15721 tgtgaaagtc aataaaatcc gtatgcggga aacagtgatt tcctacgctt tcctagcacc
    15781 agtattattc ttctttgtca tctttgtgtt ggctccgatg gtgatgggct tcattacaag
    15841 tttctttaac tactcaatga ctaaatttga gtttgtaggc ttggataact atatccgtat
    15901 gtttaaagat cctgtcttta caaaatctct gattaacaca gttattttgg ttattggatc
    15961 tgtaccagtt gttgttctat tctcactctt tgtagcatct cagacctatc atcaaaatgt
    16021 cattgccaga tccttctacc gtttcgtctt cttccttcct gttgtaacgg gtagtgttgc
    16081 cgtgacagtt gtttggaaat ggatttatga cccactatca gggattctaa actttgtcct
    16141 taagtcaagc cacatcatca gccaaaacat ttcttggttg ggagataaaa actgggcatt
    16201 gatggcgatt atgattattc tcttgaccac ttcagttggt cagcccatca tcctttatat
    16261 cgctgccatg gggaatattg acaattcact ggttgaagcg gcgcgtgttg atggtgcaac
    16321 tgagtttcaa gttttctgga agattaaatg gccaagcctt cttccaacaa ctctttatat
    16381 tgcaatcatc acaacaatta actcattcca gtgtttcgcc ttgattcagc ttttgacatc
    16441 tggtggtcca aactactcaa caagtacctt gatgtactac ctttacgaaa aagccttcca
    16501 attgacagaa tacggctatg ccaacacaat tggtgtcttc ttggcagtca tgattgctat
    16561 cgtaagcttt gttcaattta aagtacttgg aaacgacgta gaatactaaa gaaaggagac
    16621 agctatgcaa tctacagaaa aaaaaccatt aacagccttt actgttattt caacaatcat
    16681 tttgctcttg ttgactgtgc tgttcatctt tccattctac tggattttga caggggcatt
    16741 caaatcacaa cctgatacaa ttgttattcc tcctcagtgg ttccctaaaa tgccaaccat
    16801 ggaaaacttc caacaactca tggtgcagaa ccctgccttg caatggatgt ggaactcagt
    16861 atttatctca ttggtaacca tgttcttagt ttgtgcaacc tcatctctag caggttatgt
    16921 attggctaaa aaacgtttct atggtcaacg cattctattt gctatcttta tcgctgctat
    16981 ggcgcttcca aaacaagttg tccttgtacc attggtacgt atcgtcaact tcatgggaat
    17041 ccacgatact ctctgggcag ttatcttgcc tttgattgga tggccattcg gtgtcttcct
    17101 catgaaacaa tttagtgaaa atattcctac agagttgctt gaatcagcta aaatcgacgg
    17161 ttgtggtgag attcgtacct tctggagtgt agccttcccg attgtgaaac cagggtttgc
    17221 agcccttgca atctttacct tcatcaatac ttggaatgac tacttcatgc aattggtaat
    17281 gttgacttca cgtaacaatt tgaccatctc acttggggtt gcgaccatgc aggctgaaat
    17341 ggcaaccaac tatggtttga ttatggcagg agctgccctt gctgctgttc caatcgtcac
    17401 agtcttccta gtcttccaaa aatccttcac acagggtatt actatgggag cggtcaaagg
    17461 ataatactct gcgaaaatcg aatgcaaact acgtcagctt caccttgcca tacttaagta
    17521 ttgcctgcgg ttagcttcct agtttgttct tcaattttca ttgagtatag gaaaatcaat
    17581 ctatcaagat acagaagtat attttataga tttagagaat atagaggtta taagtgtcta
    17641 caaaatggag ggtatgcagt tactttatga agttttgtca gacacttatc aacttaagaa
    17701 tggttttagt taactatcag aaacgaagga aagagtatga tttttgacga tttgaaaaac
    17761 atcacctttt acaaagggat tcatcccaat ttagacaagg ctatcgacta cctctaccaa
    17821 caccgtaaag attcattcga attaggaaag tatgagattg atggagataa agtctttcta
    17881 gttgttcagg aaaatgtcct caatcaagtt gagaataatc aatttgaaca ccataagaac
    17941 tatgcagatt tgcatttgct gatagaaggg catgaatatt cgagctacgg ttcacgtatc
    18001 aaagacgagg cagtagcatt cgacgaagcg agtgacattg gctttgttca ttgtcatgaa
    18061 cactacccgc tcttgttggg ttatcacaat tttgcgattt tcttcccagg tgagccacat
    18121 cagccaaatg gttatgcagg catggaagaa aaggttcgaa aatatctctt taaaatcttg
    18181 attgattaaa aataggatga attgtttttt tgtaaagctt tgataatact ctaccatgaa
    18241 attgatcttt gtgaggtaga gaaatgagaa taaaatattt aaaaattggt atcttcttgt
    18301 cagtctgtgt atgctacaag agctagtttc ttggatggac agggaattac agttgatgag
    18361 atggcttgga taattagggg cattgtgaat gcattgattg gtagatacat aaaattaggt
    18421 acttatgcag ctaagtatgg tattagtatg gcgcgttcga tcttaagtag ggcagctgca
    18481 actgcggcag caagagtagg attattgact aagatttttg gatggatttt acgaatagct
    18541 gtgctcgcag ctgatgtata tggtaatttt gccaacaata ttgctgcagc ttgggatgca
    18601 catgataaaa tccctaacaa tggtcgtata aacttttaaa atgcgagaat gaaagcactt
    18661 tgtatttttt attgtatatg ttagcttgga cagtgcttgc aatgataatc cgtggagggc
    18721 tagatggatt tgatgaacac acttggcgta atattttagc tagtgcgttg tcctcgctat
    18781 acatacatta tatatccata gatagaaaaa gaaaaaagtc caaaagaaaa aagaaaaaat
    18841 agatttgttc atggtaggga cttatgaaag ctttactaac aaaaaagaaa acagtttaca
    18901 aagaaaaatg atggaggagt aaacatggca caaaaaggag taagccttat caaggcagca
    18961 tttgatacag ataactttct tatgcgtttt agtgagaaag tcttggacat cgtgacagcc
    19021 aatcttcttt ttgtcgtctc ttgtttgccc atcgtgacga ttgaagtggc taaaatcagc
    19081 ctctacgaga ccatgtttga gattaagaag agcagacggg tgcctgtttt taaaatctat
    19141 ctaagatctt tcaagcaaaa tctgaaacta ggtcttcagt taggtctgct tgagttgggc
    19201 attgtttcat taagcctcct agatctttat ctcttttggg gtcaaacagc tctgcccttt
    19261 caattgctga aagctatttg tctaggcatt ctgattttcc ttacgattgt gatgttggct
    19321 agctatccaa tcgcggcacg ttatgattta acttggaaag aagtgttaca aaaagggctt
    19381 atcttggcaa gttttaactt tccttggttc ttcctcatgt tagccattct tgtcctcatt
    19441 gtgatggttc tttatctgtc cgccttcagt ctactcttag gtggctcagt cttcctactt
    19501 tttgggtttg gactattggt ctttatccag actggattga tggagaaaat tttcgcaaaa
    19561 taccaatagg agccttattt ctgaaactac tttcaaaggc tccaaacgct attctataag
    19621 cgagaaacta aaatctaagt gtctataaga tattgaaagc cttttcggta aagtgtatac
    19681 taaagaagta aagaaaaatc tgctttagca gaaaataaaa ataataggag aaaatctatg
    19741 tcagatttga aaaaatacga aggtgtcatt ccagccttct acgcatgtta tgatgatcaa
    19801 ggagaagtaa gcccagaacg tacgcgtgcc ttggttcaat acttcattga taaaggtgtt
    19861 caaggtcttt atgtcaatgg ttcttctggt gaatgtatct accaaagcgt tgaagatcgc
    19921 aagttgattt tggaagaagt catggcggta gctaaaggta aattgaccat tattgcccat
    19981 gttgcctgca acaatactaa agacagtatg gaacttgctc gccatgctga aagcttggga
    20041 gtagatgcta ttgcaacgat tccaccaatt tatttccgct tgccagaata ctcagttgcc
    20101 aaatactgga acgatatcag ttctgcagct ccaaacacag actacgtgat ttacaacatt
    20161 cctcaattgg caggggttgc tttgactcca agcctttaca cagaaatgtt gaaaaatcct
    20221 cgtgttatcg gtgttaagaa ctcttctatg ccagttcaag atatccaaac ctttgtcagc
    20281 cttggtgggg aagaccatat cgtctttaat ggtcctgatg agcagttcct aggaggacgc
    20341 ctcatggggg ctagggctgg tatcggtggt acttatggtg ctatgccaga actcttcttg
    20401 aaactcaatc agttgattgc ggataaggac ctagaaacag cgcgtgaatt gcagtatgct
    20461 atcaacgcaa tcattggtaa actcacttct gctcatggaa atatgtacgg tgtcatcaaa
    20521 gaagtcttga aaatcaatga aggcttgaat attggatctg ttcgttcacc attgacacca
    20581 gtaactgaag aagatcgtcc agttgtagaa gcggctgcag ccttgattcg tgaaaccaag
    20641 gagcgcttcc tctaatctaa aaggaggtat ttatgacata ctacgttgca attgatatcg
    20701 gtggaactaa catcaagtat ggtttggttg atcaagaggg gcaacttctt gaatcgcatg
    20761 aaatgccaac tgaggcgcat aagggtggac ctcatatctt acaaaagacc aaagatatcg
    20821 tagctagtta tttagaaaaa ggcccagtag caggtgttgc catatcttct gctgggatgg
    20881 tggatccgga taagggtgag attttctatg ctgggccgca aatccctaac tacgcaggca
    20941 cccagttcaa aaaggaaatc gaagaaagct ttactattcc ttgtgagatt gaaaatgatg
    21001 tcaactgtgc aggtcttgct gaggcagtat ctggttcagg caagggagca agtgtgacac
    21061 tttgcttgac cattggaaca ggtatcggtg gttgcttgat tatggatagg aaagtcttcc
    21121 atggttttag taattcagcc tgtgaagtcg ggtatatgca tatgcaggat ggagcttttc
    21181 aagacttggc ttctacaaca gctttagtga aatatgtagc tgaagcccat ggagaagatg
    21241 ttgatcagtg gaatggccga cgtatcttta aggaagctac tgaaggaaac aaaatctgca
    21301 tggaaggtat tgaccgtatg gttgactatc taggaaaagg tctggcaaat atttgctacg
    21361 ttgccaatcc agaagtggtc attcttggtg gtggtatcat ggggcaagag gctatcctca
    21421 aacctaagat ccgcacagcc ttgaaagagg ctttggtacc aagtttagca gaaaaaacac
    21481 gattagaatt tgcccatcac caaaatacag cagggatgtt gggtgcatat tatcatttta
    21541 agacaaaaca atcctagttt ggctcagcca aactaggatt ttcttacacg tttttgtcta
    21601 cgatagccgt tgagtttttt attttcccag tagctattaa agattttttc cttgctttcg
    21661 cgattgattt ccaaaaagta ggcataaatc aaatcgataa agaagagcat aggaagttga
    21721 gcggatattc gttggatata ggagggttgg ctgtgggtgg ctacaagaac agtctctgta
    21781 taggtctggc tatctttatt gggaacactt gtaaagagta cagtctttgc ccccatctcc
    21841 ttggcatcta atagactatc taaaatagaa ggagttgagc ctgaaagtga gaagcccagt
    21901 actagacaat tttcatccat gatgctggtt gtccaggcaa agccgtcttg gtctgtcaaa
    21961 gcttcgcaga ccacacctag tcgcataaaa cgtaatttca tttcacgggc gacgaggcca
    22021 gaactccctg ttccaaagaa gtagatacgc tcagcatctt cgattagctg ggcaattcgc
    22081 tctagttgga tttcgtcaat caagtcttgt gtttgttccc tcatattgct ataacttctg
    22141 aggactcgtt tggtcagtgg actgtgcttg gagacttggt tggcttgatt ttctgcctga
    22201 tgttggtatt ggaaaataaa ttctcggtag ccagtaaagc cacacttttt agcaaagcgg
    22261 gtcaaagcag cttgagaaat atgtaatttt tgggtgactt gttgagaaga taaatcatct
    22321 gtaatcgttt cagcttgcaa aaaatagcga gcgatttctt gctctaggtc tgtcatttct
    22381 tcaaaatgtg aatcaatgat agttgcgata tctggtttgt ccatagggaa agctccttta
    22441 catgagtcat actggaagac tagatcaggg aatagtcaca cttcattata acacataata
    22501 taaggataga taaataaaaa cgcatttctg ttttaaaaac gaaaaaatcg aaaaagcttc
    22561 tttcttttcc ataattttct actcaaattg tggtacaatt aagagtaaga ttttaagtta
    22621 gaaatgagac tgatttgtat gagaaaattt aacagccatt cgattccgat tcggcttaat
    22681 ttattgtttt caatcgtcat tttactcttt atgaccatta ttggtcgttt gttgtatatg
    22741 caggttttga acaaggattt ttacgaaaaa aagctagcct cagctagtca gaccaagatt
    22801 acaagcagtt cagcccgtgg ggaaatttat gatgctagtg gaaaaccttt ggtagaaaat
    22861 acgttaaagc aggttgtttc ctttacgcgt agcaataaaa tgacggctac agacttaaaa
    22921 gaaacagcta aaaagttact gacttatgtg agcatcagtt ctccaaattt gacagaacgc
    22981 cagctggcgg attactattt ggctgatcct gaaatctata aaaaaacagt ggaagctctc
    23041 ccaagtgaga aacgcttgga ttcagatggc aatcgtctat ccgaatcaga actgtataac
    23101 aatgcggtcg atagtgtcca aacaagtcaa ctaaactata cagaggatga aaagaaagaa
    23161 atctatcttt ttagtcagtt aaatgctgtt ggaaactttg cgacaggaac cattgctaca
    23221 gatcctctaa atgattctca ggtggctgtt attgcctcta tttcaaagga gatgcctggc
    23281 attagtattt ctacttcttg ggatagaaag gttttggaaa cttccctttc ttctatagtt
    23341 gggagtgtat ccagtgaaaa agctggtctc ccagcggaag aagcagaagc ctatcttaaa
    23401 aaaggctatt ctctaaatga tcgtgttgga acctcctatt tggaaaagca atatgaagag
    23461 accttacaag ggaaacgctc ggtaaaagaa atccatctgg ataaatatgg caatatggaa
    23521 agtgtggata caattgagga aggtagtaag ggaaacaata tcaagctgac cattgatttg
    23581 gccttccaag atagcgtgga tgctttgctg aaaagttatt tcaattccga gctaggaaat
    23641 ggtggagcca agtattctga gggtgtgtat gcagtcgccc ttaaccccaa aacaggtgct
    23701 gttttgtcta tgtcaggact caaacatgac ctgaaaacgg gagagttgac tcctgattcc
    23761 ttgggaacgg taaccaatgt ctttgtccca ggttcggttg tcaaggctgc gaccatcagc
    23821 tctggctggg aaaatggagt cttatcagga aaccagacct tgacagacca gtccattgtc
    23881 tttcaaggtt cagctcccat caattcttgg tatactcagg cttacggttc attccctatc
    23941 acagcagtcc aagctctgga gtattcatct aatgcctata tggtccaaac agctttaggt
    24001 cttatggggc agacctacca acctaatatg tttgtcggca ccagcaacct agagtctgct
    24061 atggggaaat tgcgttcaac ctttggtgaa tatggtttgg gttctgcgac cgggattgac
    24121 ctaccagatg aatctactgg atttgttccc aaagactata gctttgctaa ttacatcacc
    24181 aatgcctttg ggcagtttga taactatacg ccgatgcagt tggctcagta tgtagcaact
    24241 attgcaaatg atggtgttcg tgtggctcct cgtattgttg aaggcattta tggaaataat
    24301 gataagggag gcctaggcga cttgattcag caactgcaac cgacagagat gaataaggtc
    24361 aatatatccg actctgatat gagtattttg caccaaggtt tttatcaggt tgctcatggg
    24421 actagtggat tgacaactgg acgtgccttt tcaaatggcg cagcggtatc cattagtgga
    24481 aaaacaggta ctgccgaaag ttatgttgag ggtggtcaag aagctaacaa tactaatgct
    24541 gtggcctatg caccatcaga taatcctcaa atcgctgtag ctgttgtctt ccctcataac
    24601 accaacctta caaatggtgt cggaccttcc attgcgcgcg atattatcaa cctctataac
    24661 caacatcatc caatgaatta gaaaggaaca tatgctttat ccaacaccta ttgctaagtt
    24721 gattgacagt tattctaagt taccaggtat cgggattaag acggctacgc gtctggcctt
    24781 ttatacgatt gggatgtctg ctgatgatgt caatgaattt gcaaaaaatc tcctttctgc
    24841 taagagagaa ttgacatatt gttctatttg tggacgtttg acagacgacg atccttgttc
    24901 tatctgtact gatccgactc gtgaccagac aacaatttta gttcttgagg atagtagaga
    24961 tgtggcagcc atggaaaata tccaagaata ccatggactc tatcatgtcc ttcatggcct
    25021 catttctcct atgaatggta tcagtccgga cgatatcaat ctcaagagcc ttatgactcg
    25081 tcttatgaat agtgaagttt cagaagtgat tgtggcgact aatgctacag cggatggtga
    25141 agcgacttcc atgtatcttt cacgtttgct caagccggct ggtatcaagg ttacgcgtct
    25201 agcacgaggt ctcgctgtgg gagcggacat tgagtatgcg gacgaagtga cactcttacg
    25261 agcgattgaa aatcggacag agttgtaagt gtaggcaaat ttacgaactc cattcattta
    25321 taaaaaatca aagaggctga aaatcgttcc tatcggcctc tttttgtata gtgtgatgag
    25381 taggatcagg ttcaagtttt aaaaaaccaa gcaaatatga tatactaaag agcgagtatt
    25441 ctagtagaat taggacaaat aatatgaaac aaacgattat tcttttatat ggtggacgga
    25501 gtgcggaacg cgaagtctct gtcctttcag ctgagagtgt catgcgtgcg gtcaattacg
    25561 accgtttcac agtcaagact ttctttatca gtcagtcagg tgactttatc aaaacacagg
    25621 aatttagtca tgctccgggg caagaagacc gtctcatgac caatgaaacc attgattggg
    25681 ataagaaagt tgcaccaagt gctatctacg aagaaggtgc agtggtcttt ccagtccttc
    25741 acgggccaat gggagaagat ggctctgttc aaggattctt ggaagttttg aaaatgcctt
    25801 acgttggttg caacattttg tcatcaagtc ttgccatgga taaaatcacg actaagcgtg
    25861 ttctggaatc tgctggtatt gcccaagttc cttatgtggc tatcgttgaa ggcgatgatg
    25921 tgactgctaa aatcgctgaa gtggaagaaa aattggctta tccagtcttc actaagccgt
    25981 caaacatggg gtctagtgtc ggtatttcta agtctgaaaa ccaagaagaa ctccgtcaag
    26041 ccttaaaact tgccttccga tatgacagcc gtgtcttggt tgagcaagga gtgaatgccc
    26101 gtgaaattga ggttggcctc ttgggtaact acgatgtcaa gagcacgcta cctggagaag
    26161 ttgtcaagga cgttgccttt tatgactacg atgccaagta tattgataac aagattacta
    26221 tggatattcc tgccaaaatc agtgatgatg tggtggctgt catgcgtcaa aatgcagaaa
    26281 cagccttccg tgccattggt ggccttggtc tatctcgttg cgatttcttc tatacagata
    26341 agggagagat ttttctcaac gagctcaata ccatgccagg ttttacccag tggtctatgt
    26401 acccactact ttgggacaat atggggatca gctacccaga actaatcgag cgtttggttg
    26461 accttgccaa ggaaagtttt gacaagcgcg aagcgcattt gatataaaat gaaagagagg
    26521 gtagaagcca gaaccatcac tgcacggtga ctagagttct cggacttcag ccctttttaa
    26581 aggagtagaa atgaaattaa caatccatga aattgcccaa gttgttggag ccaaaaatga
    26641 tatcagtatc tttgaggaca cccagttaga aaaagctgag tttgatagtc gtttgattgg
    26701 aactggagat ttatttgtgc cacttaaagg tgcgcgtgat ggccatgact ttattgaaac
    26761 agcctttgaa aatggtgcag cagtaacctt gtctgagaaa gaggtctcaa atcatcctta
    26821 cattctagta gatgatgttt tgacagcctt tcaatcccta gcatcctact atcttgaaaa
    26881 aacgactgtt gatgtctttg ctgttacagg ttcaaatggc aagacaacga ctaaggatat
    26941 gttggcgcat ttactgtcaa caagatacaa gacctacaaa acacaaggca attacaataa
    27001 tgagattggc cttccttaca cagttctcca catgcctgaa ggaacagaaa agttggtctt
    27061 ggagatgggg caggaccact tgggagatat tcatctcttg tctgaattgg ctcgtccaaa
    27121 aacagccatc gtgaccttgg ttggagaagc tcatttggcc tttttcaaag atcgttctga
    27181 gattgctaaa ggaaaaatgc aaattgcaga cggaatggct tcaggttcct tgcttttagc
    27241 gccggctgac cctatcgtag aggactattt gccaactgat aaaaaggtgg ttcgttttgg
    27301 tcaaggagca gagctggaaa ttactgactt ggttgagcgc aaagatagtc tgaccttcaa
    27361 ggccaatttc ttagagcaag tccttgattt gccagtaact ggcaagtata atgcgacaaa
    27421 tgctatgatt gcatcctatg ttgccttgca agaaggagtt tcagaggagc aaattcatca
    27481 ggccttccaa gatcttgaat tgacgcgtaa ccgtaccgaa tggaagaaag cagccaatgg
    27541 agcagatatc ctgtctgatg tatacaatgc caatccaact gctatgaagt tgattttaga
    27601 gactttctct gccattccag ccaatgaagg tggcaagaaa attgcggttt tagcggacat
    27661 gaaggaactg ggtaaccagt cagttcaact tcataatcag atgattttga gcctttctcc
    27721 agatgtgctt gataccgtga ttttctatgg agaagacatt gctgaattag cccaattggc
    27781 cagtcaaatg ttcccaattg gccacgttta ctacttcaag aaaacagaag accaagacca
    27841 atttgaagac ctagtcaagc aggtcaagga aagcctcagt gccaatgatc aaatcttgct
    27901 caagggctct aactctatga acctagctat gttggtagaa agtttagaaa atgaaaccaa
    27961 gtgattttgt taagtatctg caaagaatga ttgcccttac agatactggc ttaaccttta
    28021 caaaagatcc ttttgaccgt gagcgctacg aggacttgcg aagtctgtta tctgaaatgt
    28081 tgaatcaggg atcagacctg gatgcagaag aagtagcaga agtcttgaaa ccaacttcag
    28141 cttatgcgac tcccttaatg gacgtccgtg cttggattgt tgaggatgag aagatttgtc
    28201 tggttagggg acaaggagag ggtagttggg ctttgccagg tggctttggt gaagtcggct
    28261 attctccaac tgaaaatatt ctcaaggaaa ttgaagaaga aaccggtttt aaagccaaag
    28321 ttgaaagact gctagctgtt tttgatatca atcgtttcca actacagagc aaacaatatg
    28381 caaagtttgt ttttgaatgt aagcttctcg atggacaatt ccaagaaaat caagaaattg
    28441 cagaccttca attttttgcc attgatcaac tgccagtctt atctgaaaaa cgcattacca
    28501 aggagcaaat agagattctt tggcaggttt atcaaggtca gagggagcaa tatcttgact
    28561 aagaagatga ttatcgtatt tttaaatcca tttttaacaa ctagcatggt ataataatat
    28621 gcaggaaaat tttgaattat gaggaagact agatgaattt atgggatatt ttctttacga
    28681 ctcaggcaac caagccgccc aaatttgacc ttttttggta tgttagccta tttacgctct
    28741 tagccttaac cttttataca gcccatcgct atcgtgaaaa gaaggtttac caacgatttt
    28801 tccaaatctt gcagactgtt cagttaatcc ttctttatgg ttggtactgg gtcaatcata
    28861 tgccactgtc agaaagccta cccttttacc attgccgtat ggctatgttt gtggtactct
    28921 tgcttcctgg tcaatccaaa tataaacaat actttgcatt attgggaaca tttgggacat
    28981 tagcagcctt tgtttatcca gtgccagatg cttacccttt tccacatatc accattctat
    29041 cctttatctt tggtcattta gcactcttgg ggaactctct agtttatcta ttgagacagt
    29101 ataatgcgcg attgctggat gtgaagggaa tttttctcat gacctttgcc ctaaatgcct
    29161 tgatttttgt ggtcaatttg gtgacaggtg gcgattacgg atttttgaca aaaccgccat
    29221 tggttgggga tcacggtcta gtagctaatt atttacttgt ttcaattgtg ctggtagcta
    29281 ctatcagttt gactaagaaa atcttagaat tctttttagc tcaagaagca gaaaaaatga
    29341 ttgtaaagga agcttaacac agagctttct tttttgctct tagagagttt ttacaatcag
    29401 cttataaaat aagaatttct gaatagacaa actcaaaaaa tggctgggaa atttaggaaa
    29461 aaagcaagca cgattaaatt ttttgtgtta taatattttg tgaatagcta tgcctatgtt
    29521 tagctatgga ataatacgaa gtgcgaaact tggaagatag agaggaagcg atgtaatggc
    29581 tagagaaggc ttttttacag gtctagatat tggaacaagc tctgtcaagg tgcttgtggc
    29641 cgagcagaga aatggtgaat taaatgtaat tggcgtgagt aatgccaaaa gtaaaggtgt
    29701 aaaggatgga attattgttg atattgatgc agcagcaact gctatcaagt cagccatttc
    29761 ccaagcggaa gaaaaggcag gcatttcgat taaatcagta aatgtcggct tgcctggtaa
    29821 tcttttgcag gtagaaccaa ctcaggggat gattccagta acatctgata ctaaggaaat
    29881 tacggatcaa gatgttgaaa atgttgtcaa atcagctttg acaaagagta tgacacctga
    29941 ccgtgaagtc attaccttta ttcctgaaga atttattgtg gatggtttcc aagggattcg
    30001 tgacccacgt ggcatgatgg gggttcgcct tgaaatgcgt ggtttgcttt atacaggacc
    30061 tcgtactatc ttgcacaatt tgcgtaagac ggttgagcgt gcaggtgttc aggttgaaaa
    30121 tgttatcatt tcaccactag caatggttca gtctgttttg aacgaagggg aacgtgaatt
    30181 tggtgctaca gtgattgata tgggggcagg tcaaacgact gtcgctacaa tccgtaatca
    30241 agaactccag ttcacacata ttctccaaga aggtggagat tatgtaacta aagatatctc
    30301 caaggttttg aaaacctctc gcaaattagc ggaaggcttg aaactgaatt acggggaagc
    30361 ctatccgcct cttgcaagca aagaaacctt ccaagtagag gttattggag aagtagaagc
    30421 agtcgaagtg acggaagcct acttgtcaga aattatttct gcacgaatca agcacatcct
    30481 tgaacaaatc aagcaagaat tagatagaag gcgtctattg gacctccctg gtggtattgt
    30541 cttaatcggt gggaatgcca ttttaccagg tatggttgag cttgctcagg aagtctttgg
    30601 cgtccgtgtc aagctttatg ttccaaatca agttggtatc cgtaatccag cctttgcgca
    30661 tgtgattagt ttatcagaat ttgcgggtca attaacagaa gttaatcttt tggctcaggg
    30721 agcgataaaa ggtgagaatg acttaagtca tcagccaatt agttttggtg ggatgctgca
    30781 aaaaacagct cagtttgtac aatcaacgcc tgttcaacca gctcctgctc cagaagtaga
    30841 gccggtggcg cctacagaac caatggcgga tttccaacaa gcttcacaaa ataaaccgaa
    30901 attagcagat cgtttccgtg gcttgatcgg aagcatgttt gacgaataaa gaggaaaaat
    30961 aaattatgac attttcattt gatacagctg ctgctcaagg ggcagtgatt aaagtaattg
    31021 gtgtcggtgg aggtggtggc aatgccatca accgtatggt cgacgaaggt gttacaggcg
    31081 tagaatttat cgcagcaaac acagatgtac aagcattgag tagtacaaaa gctgagactg
    31141 ttattcagtt gggacctaaa ttgactcgtg gtttgggtgc aggaggtcaa cctgaggttg
    31201 gtcgtaaagc cgctgaagaa agcgaagaaa cactgacgga agctattagt ggtgccgata
    31261 tggtcttcat cactgctggt atgggaggag gctctggaac tggagctgct cctgttattg
    31321 ctcgtattgc caaagattta ggtgcgctta cagttggtgt tgtaacacgt ccctttggtt
    31381 ttgaaggaag taagcgtgga caatttgctg tagaaggaat caatcaactt cgtgagcatg
    31441 tagacactct attgattatc tcaaacaaca atttgcttga aattgttgat aagaaaacac
    31501 cgcttttgga ggctcttagc gaagcggata acgttcttcg tcaaggtgtt caagggatta
    31561 ccgatttgat taccaatcca ggattgatta accttgactt tgccgatgtg aaaacggtaa
    31621 tggcaaacaa agggaatgct cttatgggta ttggtatcgg tagtggagaa gaacgtgtgg
    31681 tagaagcggc acgtaaggca atctattcac cacttcttga aacaactatt gacggtgctg
    31741 aggatgttat cgtcaacgtt actggtggtc ttgacttaac cttgattgag gcagaagagg
    31801 cttcacaaat tgtgaaccag gcagcaggtc aaggagtgaa catctggctc ggtacttcaa
    31861 ttgatgaaag tatgcgtgat gaaattcgtg taacagttgt tgcaacgggt gttcgtcaag
    31921 accgcgtaga aaaggttgtg gctccacaag ctagatctgc tactaactac cgtgagacag
    31981 tgaaaccagc tcattcacat ggctttgatc gtcattttga tatggcagaa acagctgaat
    32041 tgccaaaaca aaatccacgt cgtttggaac caactcaggc atctgctttt ggtgattggg
    32101 atcttcgccg tgaatcgatt gttcgtacaa cagattcagt cgtttctcca gtcgagcgct
    32161 ttgaagcccc aatttcacaa gatgaagatg aattggatac acctccattt ttcaaaaatc
    32221 gttaagtaaa tgaatgtaaa agaaaataca gaacttgttt ttcgagaagt tgcagaggct
    32281 agtctgagtg ctcatcgaga gagtggttcg gtctctgtca ttgcagttac caagtatgta
    32341 gatgtaccga cagcggaagc cttgcttccg ctaggtgttc atcatatcgg tgaaaatcgt
    32401 gtagataagt ttctggaaaa atatgaagct ttaaaagatc gagatgtgac ttggcatttg
    32461 attggtacct tgcaaagacg taaggtgaaa gatgtcattc aatacgttga ttatttccat
    32521 gcattggact cagtaaagct agcaggggaa attcaaaaaa gaagtgaccg agtcatcaag
    32581 tgtttccttc aagtaaatat ttctaaagaa gaaagcaaac acggtttttc gagagaggaa
    32641 ctgctggaaa tcttgccaga gttagccaga ctagataaga ttgaatatgt tggtttaatg
    32701 acgatggcac cttttgaggc tagcagtgag cagttgaaag agattttcaa ggcggcccaa
    32761 gatttacaaa gagaaattca agagaaacaa attccaaata tgcctatgac cgagttaagt
    32821 atgggaatga gtcgtgatta taaagaagcg attcaattcg gttccacttt tgttcgtata
    32881 ggtacatcat tttttaagta ggagagaacc atgtctttaa aagatagatt cgatagattt
    32941 atagattatt ttacggagga tgaggattca agtctccctt atgaaaaaag agatgagcct
    33001 gtgtttactt cagtaaattc ttcacaggaa ccggctctcc caatgaatca accttcacag
    33061 tcggctggta caaaagagaa caatatcacc agacttcatg caagacaaca ggaattggca
    33121 aatcagagtc agcgtgcaac ggataaggtc attatagatg ttcgttatcc tagaaaatat
    33181 gaggatgcaa cagaaattgt tgatttattg gcaggaaacg aaagtatctt gattgacttt
    33241 cagtatatga cagaggtgca ggctcgtcgt tgtttggact atttggatgg agcttgtcat
    33301 gttttagctg gaaatttgaa aaaggtagct tctaccatgt atttgttgac accagtgaac
    33361 gttattgtaa atgttgaaga tatccgttta ccagatgaag atcaacaggg tgagttcggt
    33421 tttgatatga agcgaaatag agtacgataa tgattttttt aattcgtatg atttataatg
    33481 cagtggatat ttactccctg attttggtag ccttcgctgt catgtcttgg tttccaggtg
    33541 cctacgaatc cagtttaggt cgttggattg tagcgttggt gaaaccagtg cttgctccct
    33601 tgcaacgcct gcctttacag atagcgggtc ttgatttatc tgtttgggtt gcgattgttt
    33661 tggttcgatt tttaggagaa aacctagtgc gttttctggc gatgatagga tgaataaagg
    33721 gatttatcag catttctcca tagaagatcg tccatttctt gacaagggaa tggaatggat
    33781 aaagaaggta gaagatagct atgctccttt tttaactcct tttatcaatc ctcatcagga
    33841 gaagctatta aagattttgg ccaaaaccta tggtcttgct tgtagcagta gtggggaatt
    33901 cgtctcgagt gagtatgttc gagttttatt atacccagat tatttccaac cagagttttc
    33961 agattttgaa atatctctcc aggaaattgt gtattccaat aaatttgaac atttaacgca
    34021 tgctaagatt ttagggacag tcatcaatca attagggatt gaacggaaac tttttggaga
    34081 tatcctagta gatgaagaac gggcgcagat tatgattaat cagcagtttc ttcttctctt
    34141 tcaagatgga ctaaagaaaa ttggtcgtat acctgtttcg ctggaggaac gtcctttcac
    34201 cgagaaaata gataagctag aacagtatcg agaactggat ttatctgtgt ctagttttcg
    34261 attagatgtt cttttatcaa atgttttgaa actatctagg aatcaagcaa accagttgat
    34321 tgaaaagaaa cttgtccaag taaattatca tgtggtagac aaatcagatt acactgttca
    34381 agttggagac ttgattagtg tgagaaaatt tggtcgcttg agattacttc aagataaggg
    34441 acaaacgaaa aaagagaaga aaaaaataac cgtccagtta ttattaagta agtgaggaat
    34501 agaatgccaa ttacatcatt agaaataaag gacaagactt ttggaactcg attcagaggt
    34561 tttgatccag aagaagtcga tgaattttta gatattgtgg ttcgtgatta cgaagatctt
    34621 gtgcgtgcga atcatgataa aaatttgcgt attaagagtt tagaagagcg tttgtcttac
    34681 tttgatgaaa taaaagattc attgagccag tctgtattga ttgctcagga tacagctgag
    34741 agagtgaaac aggcggcgca tgaacgttca aacaatatca ttcatcaagc agagcaagat
    34801 gcgcaacgct tgttggaaga agctaaatat aaggcaaacg agattcttcg tcaagcaact
    34861 gataatgcta agaaagtcgc tgttgaaaca gaagaattga agaacaagag ccgtgtcttc
    34921 caccaacgtc tcaaatctac aattgagagt cagttggcta ttgttgaatc ttcagattgg
    34981 gaagatattc tccgtccaac agctacttat cttcaaacca gtgatgaagc ctttaaagaa
    35041 gtggttagcg aagtacttgg agaaccgatt ccagctccaa ttgaagaaga accaattgat
    35101 atgacacgtc agttctctca agcagaaatg gcagaattac aagctcgtat tgaggtagcc
    35161 gataaagaat tgtctgaatt tgaagctcag attaaacagg aagtggaaac tccaactcct
    35221 gtagtgagtc ctcaagttga agaagagcct ctgctcatcc agttggccca atgtatgaag
    35281 aaccagaagt agctccagtg catccgacag gtccaacacc agctacagaa actgttgatt
    35341 cagcaccggg atttgaagca ccgcaagaat ctgttacaat tttataagaa atattctgag
    35401 aacaatatct tatccttata ttcccagcga gcaggagatg gtgtgagtcc tgcattccct
    35461 atcgataaga ttatcctctc aaactatcaa gtctgaatct agtaagattt gacgttcccc
    35521 acgttacggg ataagaggga gaaagactaa aactttttcc gaataaaggt ggtaccacga
    35581 ttttcgtcct ttttggaagt cgtggttttt aatttgttat tatttataaa ggagatacca
    35641 tgaaactcaa agacaccctt aatcttggga aaactgaatt cccaatgcgt gcaggccttc
    35701 ctaccaaaga gccagtttgg caaaaggaat gggaagatgc aaaactttat caacgtcgtc
    35761 aagaattgaa ccaaggaaaa cctcatttca ccttgcatga tggccctcca tacgctaacg
    35821 gaaatatcca cgttggacat gctatgaaca agatttcaaa agatattatt gttcgttcta
    35881 agtctatgtc aggattttac gcaccattta ttcctggttg ggatactcat ggtctgccaa
    35941 tcgagcaagt attgtcaaaa caaggtgtca aacgtaaaga aatggacttg gttgagtact
    36001 tgaaactttg ccgtgagtac gctctttctc aagtagataa acaacgtgaa gattttaaac
    36061 gtttgggtgt ttctggtgac tgggaaaatc catatgtgac cttgactcct gactatgaag
    36121 cagctcaaat tcgtgtattt ggtgagatgg ctaataaggg ttatatctac cgtggtgcca
    36181 agccagttta ctggtcatgg tcatctgagt cagcccttgc tgaagcagag attgaatacc
    36241 atgacttggt ttcaacttcc ctttactatg ccaacaaggt aaaagatggc aaaggagttc
    36301 tagatacaga tacttatatc gttgtctgga caacgactcc atttaccatc acagcttctc
    36361 gtggtttgac ggttggtgca gatattgatt acgttttggt tcaacctgtt ggtgaagctc
    36421 gtaagtttgt cgttgctgct gaattattga ctagcttgtc tgagaaattt ggctgggctg
    36481 atgttcaagt tttggaaact taccgtggcc aagaactcaa ccacatcgta acagaacacc
    36541 catgggatac agctgtagaa gagttggtaa ttcttggtga ccacgttacg actgactctg
    36601 gtacaggtat tgtccataca gcccctggtt ttggtgagga cgattacaat gttggtattg
    36661 ctaataatct tgaagtcgca gtgactgttg atgaacgtgg tatcatgatg aagaatgctg
    36721 gtcctgaatt tgaaggtcaa ttctatgaaa aggtagttcc aactgttatt gaaaaacttg
    36781 gtaacctcct tcttgcccaa gaagaaatct ctcactcata tccatttgac tggcgtacta
    36841 agaaaccaat catctggcgt gcagttccac aatggtttgc ctcagtttct aaattccgtc
    36901 aagaaatctt ggacgaaatt gaaaaagtga aattccactc agaatggggt aaagtccgtc
    36961 tttacaatat gatccgtgat cgtggtgact gggttatctc tcgtcaacgt acttggggtg
    37021 ttccacttcc tatcttctac gctgaagatg gtacagctat catggtagct gaaactattg
    37081 aacacgtagc tcaacttttt gaaaaacatg gttcaagcat ttggtgggaa cgtgatgcca
    37141 aagacctctt gccagaagga tttactcatc caggttcacc aaacggcgag ttcaaaaaag
    37201 aaactgatat catggacgtt tggtttgact caggttcatc atggaatgga gtggtggtaa
    37261 accgtcctga attgacttac ccagccgacc tttacctaga aggttctgac caataccgtg
    37321 gttggtttaa ctcatcactt atcacatctg ttgccaacca tggcgtagca ccttacaaac
    37381 aaatcttgtc acaaggtttt gcccttgatg gtaaaggtga gaagatgtct aaatctcttg
    37441 gaaatactat tgctccaagc gatgttgaaa aacaattcgg tgctgaaatc ttgcgtctct
    37501 gggtaacaag tgttgactca agcaatgacg tgcgtatctc tatggatatc ttgagccaag
    37561 tttctgaaac ttaccgtaag attcgtaaca ctcttcgttt cttgattgcc aatacatctg
    37621 actttaaccc agctcaagat acagtcgctt acgatgagct tcgttcagtt gataagtaca
    37681 tgacgattcg ctttaaccag cttgtcaaga ccattcgtga tgcctatgca gactttgaat
    37741 tcttgacgat ctacaaggcc ttggtgaact ttatcaacgt tgacttgtca gccttctacc
    37801 ttgattttgc caaagatgtt gtttacattg aaggtgccaa atcactggaa cgccgtcaaa
    37861 tgcagactgt cttctatgac attcttgtca aaatcaccaa actcttgaca ccaatccttc
    37921 ctcacactgc ggaagaaatt tggtcatatc ttgagtttga aacagaagac ttcgtccaat
    37981 tgtcagaatt accagaagtt caaacttttg ctaaccaaga agaaatcttg gatacatggg
    38041 cagccttcat ggactttcgt ggacaagcac aaaaagcctt ggaagaagct cgtaatgcaa
    38101 aagttatcgg taaatcactt gaagcacact tgacagttta tccaaatgaa gttgtgaaaa
    38161 ctctactcga agcagtaaac agcaatgtag ctcaactttt gatcgtgtct gagttgacca
    38221 tcgcagaaga accagctccg gaagctgccc ttagcttcga agatgtagcc ttcacagttg
    38281 aacgtgctgc tggtgaagta tgtgaccgtt gccgtcgtat cgacccaaca acagcagaac
    38341 gcagctacca ggcagttatc tgtgaccact gtgcaagcat cgtagaagaa aattttgcgg
    38401 atgcagtcgc agaaggattt gaagagaaat aagattgaaa agtctaggca aaattcaatt
    38461 tgagaagaaa agacaactaa ttttatggtc tattaaacgc attgtatcac gtttttgaac
    38521 acctgatatg atgcgttttt atttattttg aaaatttgtg aggtgtgact ttttatactc
    38581 aacaagaatc aaagagaaac ttagcaagct aacagtagta agataaaata ggaatttga
//
LOCUS       DAVKWS010000013        38571 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKWS010000013 DAVKWS010000000
VERSION     DAVKWS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.013"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            189..1472
                     /locus_tag="VW852_000722"
     CDS             189..1472
                     /locus_tag="VW852_000722"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV0271899.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(1520..3886)
                     /locus_tag="VW852_000723"
     CDS             complement(1520..3886)
                     /locus_tag="VW852_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV0271900.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGEQKSHHLETIQKHITTIEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDHAIQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWNRIPAMIGITDTDLKEIFGK"
     gene            complement(3963..4091)
                     /locus_tag="VW852_000724"
                     /pseudo
     CDS             complement(3963..4091)
                     /locus_tag="VW852_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
     gene            complement(4165..4779)
                     /gene="lepB"
                     /locus_tag="VW852_000725"
     CDS             complement(4165..4779)
                     /gene="lepB"
                     /locus_tag="VW852_000725"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV0271901.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            complement(4783..5664)
                     /locus_tag="VW852_000726"
     CDS             complement(4783..5664)
                     /locus_tag="VW852_000726"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV0271902.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5751..6053
                     /gene="zapA"
                     /locus_tag="VW852_000727"
     CDS             5751..6053
                     /gene="zapA"
                     /locus_tag="VW852_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV0271903.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            6050..6598
                     /locus_tag="VW852_000728"
     CDS             6050..6598
                     /locus_tag="VW852_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV0271904.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEDQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSQKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            6702..9038
                     /locus_tag="VW852_000729"
     CDS             6702..9038
                     /locus_tag="VW852_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV0271905.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELAFWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     regulatory      9201..9289
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9380..10702
                     /locus_tag="VW852_000730"
     CDS             9380..10702
                     /locus_tag="VW852_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV0271906.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGVYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            10920..11468
                     /locus_tag="VW852_000731"
     CDS             10920..11468
                     /locus_tag="VW852_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV0271907.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            11578..12477
                     /locus_tag="VW852_000732"
     CDS             11578..12477
                     /locus_tag="VW852_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV0271908.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(12502..13776)
                     /gene="serS"
                     /locus_tag="VW852_000733"
     CDS             complement(12502..13776)
                     /gene="serS"
                     /locus_tag="VW852_000733"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV0271909.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(13990..14361)
                     /locus_tag="VW852_000734"
     CDS             complement(13990..14361)
                     /locus_tag="VW852_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV0271910.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIARKKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(14364..15728)
                     /locus_tag="VW852_000735"
     CDS             complement(14364..15728)
                     /locus_tag="VW852_000735"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV0271911.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            16097..16882
                     /locus_tag="VW852_000736"
     CDS             16097..16882
                     /locus_tag="VW852_000736"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV0271912.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            16976..17410
                     /locus_tag="VW852_000737"
     CDS             16976..17410
                     /locus_tag="VW852_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0271913.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            17410..18384
                     /locus_tag="VW852_000738"
     CDS             17410..18384
                     /locus_tag="VW852_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV0271914.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            18444..18668
                     /locus_tag="VW852_000739"
     CDS             18444..18668
                     /locus_tag="VW852_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0271915.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            18787..19761
                     /gene="fabK"
                     /locus_tag="VW852_000740"
     CDS             18787..19761
                     /gene="fabK"
                     /locus_tag="VW852_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV0271916.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            19754..20674
                     /gene="fabD"
                     /locus_tag="VW852_000741"
     CDS             19754..20674
                     /gene="fabD"
                     /locus_tag="VW852_000741"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV0271917.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            20708..21439
                     /gene="fabG"
                     /locus_tag="VW852_000742"
     CDS             20708..21439
                     /gene="fabG"
                     /locus_tag="VW852_000742"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV0271918.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            21461..22696
                     /gene="fabF"
                     /locus_tag="VW852_000743"
     CDS             21461..22696
                     /gene="fabF"
                     /locus_tag="VW852_000743"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV0271919.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            22699..23184
                     /locus_tag="VW852_000744"
     CDS             22699..23184
                     /locus_tag="VW852_000744"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV0271920.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            23181..23603
                     /gene="fabZ"
                     /locus_tag="VW852_000745"
     CDS             23181..23603
                     /gene="fabZ"
                     /locus_tag="VW852_000745"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV0271921.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            23615..24982
                     /gene="accC"
                     /locus_tag="VW852_000746"
     CDS             23615..24982
                     /gene="accC"
                     /locus_tag="VW852_000746"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV0271922.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            25019..25885
                     /locus_tag="VW852_000747"
     CDS             25019..25885
                     /locus_tag="VW852_000747"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV0271923.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            25882..26649
                     /locus_tag="VW852_000748"
     CDS             25882..26649
                     /locus_tag="VW852_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV0271924.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            26808..26993
                     /gene="briC"
                     /locus_tag="VW852_000749"
     CDS             26808..26993
                     /gene="briC"
                     /locus_tag="VW852_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV0271925.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            27011..27484
                     /locus_tag="VW852_000750"
     CDS             27011..27484
                     /locus_tag="VW852_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271926.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            27497..27733
                     /locus_tag="VW852_000751"
     CDS             27497..27733
                     /locus_tag="VW852_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0271927.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(27783..28205)
                     /gene="nusB"
                     /locus_tag="VW852_000752"
     CDS             complement(27783..28205)
                     /gene="nusB"
                     /locus_tag="VW852_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV0271928.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(28198..28587)
                     /locus_tag="VW852_000753"
     CDS             complement(28198..28587)
                     /locus_tag="VW852_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0271929.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(28609..29169)
                     /gene="efp"
                     /locus_tag="VW852_000754"
     CDS             complement(28609..29169)
                     /gene="efp"
                     /locus_tag="VW852_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV0271930.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(29298..30740)
                     /gene="gatB"
                     /locus_tag="VW852_000755"
     CDS             complement(29298..30740)
                     /gene="gatB"
                     /locus_tag="VW852_000755"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV0271931.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVVDFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(30740..32206)
                     /gene="gatA"
                     /locus_tag="VW852_000756"
     CDS             complement(30740..32206)
                     /gene="gatA"
                     /locus_tag="VW852_000756"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV0271932.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(32206..32508)
                     /gene="gatC"
                     /locus_tag="VW852_000757"
     CDS             complement(32206..32508)
                     /gene="gatC"
                     /locus_tag="VW852_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV0271933.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPKKDNYYIKVPAILDDGGDA"
     gene            32721..34265
                     /locus_tag="VW852_000758"
     CDS             32721..34265
                     /locus_tag="VW852_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV0271934.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            34520..34718
                     /locus_tag="VW852_000759"
                     /pseudo
     CDS             34520..34718
                     /locus_tag="VW852_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            34981..35169
                     /locus_tag="VW852_000760"
     CDS             34981..35169
                     /locus_tag="VW852_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV0271935.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            35326..35691
                     /locus_tag="VW852_000761"
     CDS             35326..35691
                     /locus_tag="VW852_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0271936.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            35694..37361
                     /locus_tag="VW852_000762"
     CDS             35694..37361
                     /locus_tag="VW852_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV0271937.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(37405..>38571)
                     /locus_tag="VW852_000763"
     CDS             complement(37405..>38571)
                     /locus_tag="VW852_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0271938.1"
                     /translation="EIIAKLDYEAPSCPDCGSLMKKYDFQKPSKIPYLETTGMPSRIL
                     LRKRRFKCYHSSKMMVAETPLVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLAISTS
                     TVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVI
                     RNHFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFR
                     VQIMNQFERKSHEYKTIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYS
                     EDLKHHYQIYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNAL
                     QLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT        11061 a         7436 c         8855 g        11219 t
ORIGIN      
        1 agataaatta gttttggaaa atgactaacc aaaagatatc caaagtagtc taaaattgtc
       61 tatactttat gagtgtttta gttaggaaaa aggcttgttg tctataattg tctgcattag
      121 tctagatttt atttatagaa aatgttataa tagactgtat ttaaaaaatt ttaaggagaa
      181 atgacagaat gtctgtatca tttgaaaaca aagaaacaaa ccgtggtgtc ttgactttca
      241 ctatctctca agaccaaatc aaaccagaat tggaccgtgt cttcaagtca gtgaagaaat
      301 ctcttaatgt tccaggtttc cgtaaaggtc accttccacg tcctatcttc gataaaaaat
      361 ttggtgaaga atcactttac caagacgtta tgaacgctct tttgccaaac gcttatgaag
      421 cagctgtaaa agaagctggt cttgaagtgg ttgcccaacc aaaaattgac gtaacttcaa
      481 tggaaaaagg tcaagactgg gttatcgctg ctgaagtcgt tacaaaacct gaagtaaaat
      541 tgggtgacta caaaaacctt gaagtatcag ttgatgtaga aaaagaagta actgacgctg
      601 atgttgaaga gcgtatcgaa cgcgaacgca acaacctggc tgaattggtt atcaaggaag
      661 ctgctgctga aaacggcgac actgttgtga tcgacttcgt tggttctatc gacggtgttg
      721 aatttgacgg cggaaaaggt gaaaacttct cacttggact tggttcaggt caattcatcc
      781 ctggtttcga agaccaattg gtaggtcact cagctggcga aaccgttgat gttatcgtaa
      841 cattcccaga agactaccaa gcagaagacc ttgcaggtaa agaagctaaa ttcgtgacaa
      901 ctatccacga agtaaaagct aaagaagttc cagctcttga cgatgaactt gcaaaagaca
      961 ttgatgaaga agttgaaaca cttgctgact tgaaagaaaa ataccgcaaa gaattggctg
     1021 ctgctaaaga agaagcttac aaagatgcag ttgaaggtgc agcaattgat acagctgtag
     1081 aaaacgctga aatcgtagaa cttccagaag aaatgatcca cgaagaagtt caccgttcag
     1141 taaatgaatt ccttgggaac ttgcaacgtc aagggatcaa ccctgacatg tacttccaaa
     1201 tcactggaac tactcaagaa gaccttcaca accaatacca agcagaagct gagtcacgta
     1261 ctaagactaa ccttgttatc gaagcagttg ccaaagctga aggatttgat gcttcagaag
     1321 aagaaatcca aaaagaagtt gagcaattgg cagcagacta caacatggaa gttgctcaag
     1381 tacaaagctt gctttcagct gacatgttga aacatgatat cacaatcaaa aaagctgttg
     1441 aattgattac aagcacagca acagtaaaat aatcttaata aacagaaaac ccacctgaat
     1501 tggtgggttt tctgatgcac tattttccaa aaatctcttt gaggtctgtg tctgtaatcc
     1561 caatcatggc tgggatgcgg ttccagtttt cttcggttag gatgtaggat tgttcagagg
     1621 cacttgatgt gactgtttca gagacagctt gttgcttttc ttcaacattc tccagtagat
     1681 cactgaagcg ttcaatcaga taggtttttc gggcagttcc gatgtgttgg atagcatggt
     1741 cgaaggcttg taattcgcct agtaagatga gtttgctttt ggcacgtgta atggctgtgt
     1801 agatgagatt tcgctccagc atacgtcggc tagcactagt aatcggtagg atgacaactg
     1861 ggaactcact tccctgagac ttatgaatac tcatggcata ggccaagcga atcttgtacc
     1921 attcgttacg ggggtaagag acttcattac catcaaaatc aatgacaatc tcgtcttgtt
     1981 tcgattcggt gtatttacca ggaatcaggt ctgtgatagc tcctaaatcc ccattaaaga
     2041 cattgatttc agcatcgtta accaaatgaa tgaccttgtc tctcttacga tagtggcact
     2101 gaggagcttc aaaactgagt tgatcttttt gtgggggatt gagcaggtct tgcatgagct
     2161 gattgatagc atcaatccct gccgtccctc ggtacatagg agccagaact tggatatcac
     2221 gggcaggaat accatttctg agggcagcac ctaagatttt ttcaatggtg gcaggaatat
     2281 ggccactagc aatttcaaag taggaacggt cagctttttt ttgggtgaaa tcagctggca
     2341 agatgccctg tcgaatctga ctagctaggg tgacgatggt tgattctttg ctttgtcgat
     2401 aaattttttc caagcgagtc tgaggaatca aaggaatatg aagcagatcc gctagaacct
     2461 gtccaggact gacagaaggt agctgatcac tgtcacctac gatgaggatc ttactgttag
     2521 aagagatatt ggagaagagt tgattggcca gccaagtatc taccatagag aattcatcca
     2581 cgatgataaa gtcagcatct aggtaatctt ccagatgact ggtatcatcg tcacctgtca
     2641 ttcccaagtg gcggtgtatg gtcgcgctag gcaaacctgt caattcattc atgcggcgag
     2701 cggctcgtcc agttggagca gcaagaagaa tgggcagatt gcttttcttc ctgaggtcaa
     2761 gtccctctaa cagggcgtaa acagcgatga tcccattgat aacagttgtc ttaccagtac
     2821 caggtccacc tgtcaggata aagaccttat tctggatagc atcacagata gcctgttttt
     2881 gaatgttatc atactcaatt cccagctctt gctcgatagt agtgatatgt ttttgaatgg
     2941 tttctaaatg atgactcttc tgttctcctt tttcaaggat acgaaccaag tgactgcgga
     3001 tgccttcctc agcgaaaaag aggctgttgt caaagatctt ggtatcaatc tgctgaacct
     3061 tgtcttcttc gatcaggtag gagagctctt gggcaacttg actggggtct agttccacgg
     3121 gacgggaaga ctcaaggaga gtaagggttt gttccagcaa atcccgtgct tcaacatagg
     3181 tgtcccctgt ttccatacag gcctgaaaaa gactgtgaac tagaccggcg cggaagcgtt
     3241 caggagcctg actttcgatg cctagttcct cagctagttg gtcagcaatg gtaaagccca
     3301 aacccttgat atcctcaact agctggtagg gataattttc aaccacatca agggtttctt
     3361 ccttgtaaaa gtcttgaatc tgaaaggcta gtttgttggg aatgccgtag ttggctagtt
     3421 tggccaaaac catctccgtt ccgtagttga gacggagagt ggagacgaaa gcctcgcgat
     3481 ttttggcaga gagtcctgcg atgccttcta acttttctgg gtgttgcaaa atttcatcaa
     3541 tggtattttc gccataggta tctacgattt tctgagctgt cttgagacca atccccttga
     3601 aatggctact tgaaaagtac ttgaccaagc ccttactagt tggttttgcg cgatcataac
     3661 gactgatttg cagttgttct ccatacttgg agtgctggac aatttgcccc caaaaagtat
     3721 agtcttcgcc ctcaattaca tcagccatgg ttcctgtgac aatgatttca aagtcatcaa
     3781 aatcctctgc gtccgtatcg tcgatttcta ggaggaggat gcgataaaaa ttgctgggat
     3841 tttcaaaaat aatccgttca atggttcctg aaaaataaac ttccataaaa ttcctttgca
     3901 tgaataggtg agagttggga ttgtttttat tttatactct tcgaaaatat cttcaaacca
     3961 cgtcagcttc catctgcaac ctcaaaacag tattttgagc tgacttcgtc agttctatcc
     4021 acaacctcaa aacactgttt taagcagcct acggctagct tcctagtttg ttctttgatt
     4081 ttcattgagt atttgtaaat aaacaatcac ttctcacgat agaagaagag gctgagattg
     4141 gtgattctcg gcctcttagg tttcttaaaa tgttccgata cgggtgattg gccataagcg
     4201 gaatttagct tcccctgtga tatcttttgc tttgaaggta cctacgtggc ggctgtcgct
     4261 cgaaaccaag cggtcatctc cgaggagaag gtattctcct tctggaacag taaagctaaa
     4321 gttggtgttg tagttgacat caactgtgaa ggcttgagct ttttgagcga tacttctaaa
     4381 gaaagttcct ttatttcctt caaagccctt gcctgagtaa gtgctttgga gtttgtcatc
     4441 cttgaagcgt ttgatatagt ctgctagata aggctcgtcc gtttctttgt cattgatgta
     4501 gagtttatca ttttcgtaac gaatggtgtc gccaggcatt ccaatcacgc gcttgacgat
     4561 gtccttattc ccatcttcct catgggccac cacgatatca aaacggtcaa taggaaggtg
     4621 ttttacaacg aagagaattt cgccatccgc tagggtcgga tccatggaat gtccttctac
     4681 gcgaacattg ctccaaaaaa agatacgact taaagctagt aatgacagaa ttaggaggaa
     4741 caatccccac tcttttaaga aatttttaaa tgaattcata acttaccttt ctaagcgttt
     4801 tttcgctttt tcagtgtttt taaagtgcaa tttggcgcag aagctgagtc cctgcatacc
     4861 ataggcttgc aaaatctggc tagccacctt gtcagaagcc gttccagctc cacttggaag
     4921 ctgataaccc agttctcgtc ccaaattttc aagattttcc agaaagagat cacgcgcaat
     4981 gacagaagaa actgcgacag ccaagtattt gccctcagcc ttttcttcta agctgatagg
     5041 attgctgaaa cgattggtct cttgtgccaa gtacttgtca taatttttag cactggtaaa
     5101 ggcatcaatc acaattttct caggctgaac acctttttga aggaggagat agatagcctg
     5161 attatggagg gcaaccttaa ccgaaacagc attgtagcgg tctccgatga cctcgttgta
     5221 cttgctgggt gagagaagga gtgcctggtg ctggattttt tccttgagaa taggagcaat
     5281 ctgacggatc ttttggtcgg tcagagtctt agaatccccc acaccgagtt ttcgtaaaaa
     5341 gtcgtgctgg tcaggtgtga caaaggcagc cacaactgca agcccaccaa agtaggaacc
     5401 atttcccacc tcatctgtcc caattaaagg aagattttgt ccgctggttt gctctacagc
     5461 ttgatagcca aagaaactgg cgtatttttc agccccttca ccctgaagca agatttttcc
     5521 agaagtatag atagaaaccg ttgcttgagg tagtttcaaa aagtagcgga tatagggatt
     5581 cttgctagga gccagactgg tttgatagtg ttcaagaaaa gcctgaatat ccttttcgct
     5641 tggtgtgagt gtgatacttg ccatagtttc tattgtacca caaaagcagt aaaatttgta
     5701 aaaactgaca aaattagcga attttggtat aatatcgtga ggtgaatttt atggcaaatc
     5761 taaatcgatt caaatttaca ttcgggaaaa aatcgttaac cttgacaagc gaacatgata
     5821 acctttttat ggaggaaatc gctaaggttg cgacagaaaa ataccaagca attaaagaac
     5881 aaatgcctag cgcagatgat gaaacaatcg ctcttttgtt ggcagtcaac tgtttatcaa
     5941 ctcagctcag ccgtgagatt gaatttgacg ataaggagca agagctagaa gaactccgtc
     6001 acaagcttgt gacttgtaag caagaacaga gcaagattga ggattcctta tgatttcatt
     6061 ccttcttcta ttggtcttgg tttggggatt ttatatcggc tatcggagag gcctgctctt
     6121 acaggtttat tacctgattt cagccatggc atcggctttt atggctggcc agttttataa
     6181 ggggcttgga gagcaattcc atttattgct cccttatgca aattcgcagg aagatcaggg
     6241 gactttcttt ttcccatcgg atcaactctt tcagctggat aaggtctttt atgcaggtat
     6301 cggctacttg cttgtatttg ggattgtcta tagcattggt cgtttgcttg gtcttctctt
     6361 acacttgatt cctagccaaa aactgggtgg taagttgttc caagtttcag caggtatctt
     6421 gtccatgttg gtgaccttat ttgtcttgca aatggccttg acaatcttgg cgaccatccc
     6481 catggcagtt atacaaaatc ctcttgaaaa gagtatcgtc gcaaaacaca tcatccagag
     6541 cataccgata acaaccagtt ggctcaaaca aatctgggtg acaaatttaa tcggataaaa
     6601 agggcaggag ttttcctagc cctttgttta cagatttgac tcgaatctat cagaatgtaa
     6661 aaagctacca cacctagaca ttcaaagaca aggaaataaa gatgaataag aaaatattag
     6721 aaacattaga gttcgataag gtcaaggcct tgtttgagcc tcatttgttg accgagcagg
     6781 gcttggagca attgagacaa ctggctccga ctgccaaagc agataaaatc aaacaggctt
     6841 ttgctgagat gaaggaaatg caggctcttt tcgtcgagca accgcatttt actattctct
     6901 caactaagga aattgcagga gtctgcaaga ggttggagat gggagcggat ctcaatatcg
     6961 aggagttcct actcttgaaa cgcgtgcttc ttgccagccg agaacttcaa agtttttacg
     7021 ccaatctgga aaatgtcagc ttggaagaat tagccttttg gtttgagaaa ttacatgatt
     7081 ttccgcaatt acaaggaaat cttcaggcct ttaatgatgc gggtttcatt gaaaattttg
     7141 ccagtgaaga attggcgcga atccgtcgaa aaatacatga tagcgagagt caggtacgcg
     7201 atgttttaca agacttgctc aagcaaaaag cgcagatgtt gacggaagga attgttgcta
     7261 gcagaaatgg ccgtcaggtt ttaccagtca aaaacaccta ccgcaataag attgcaggtg
     7321 tcgttcatga tatttctgct agtggaaaca ccgtctatat cgaaccacgt gaggtagtca
     7381 aactgagcga agaaattgct agtctgcgag cagatgagcg ctatgaaatg cttcgcattc
     7441 tccaagaaat ttctgagcgt gtccgccctc atgcggctga gattgctaat gacgcttgga
     7501 ttatcggtca tctggacttg attcgtgcca aggttcgatt tatccaagaa agacaagcag
     7561 tcgtgcctca gctgtcagaa aatcaagaga ttcaactgct ccatgtctgc catcctttgg
     7621 tcaaaaatgc cgtcgcaaat gatgtccatt ttggtcaaga tttaacagct attgtcatta
     7681 caggtcccaa tacaggtggg aagaccatca tgctcaaaac tctgggcttg acacaggtca
     7741 tggcccagtc aggattgccg attttagcag acaagggaag tcgtgttggt atttttgaag
     7801 aaatctttgc tgatattgga gatgagcagt ctattgagca gagcttgtct accttctcta
     7861 gccatatgac caatatcgtg gatattcttg gtaaggtcaa ccaacattca ctcttacttt
     7921 tggatgagtt gggggctggt actgatcccc aagagggagc agcccttgcc atggctattc
     7981 tggaggacct tcgcctgcgt caaatcaaga ccatggcgac gacccactat ccagaactca
     8041 aggcctacgg tattgagaca gcctttgtgc aaaatgccag tatggagttt gatactgcaa
     8101 ctcttcgccc gacctatcgc tttatgcagg gtgttcctgg ccgaagtaat gcctttgaaa
     8161 ttgccaaacg tctaggccta tctgaagtta tcgtaggaga tgccagtcag cagatcgatc
     8221 aggacaatga cgtcaatcgt atcattgagc aattagaaga gcagacgctg gaaagccgca
     8281 aacgtttgga caatatccgt gaggtggagc aagaaaatct caagatgaac cgtgcgctaa
     8341 aaaaactcta caacgagctt aatcgtgaaa aggaaaccga gcttaacaag gcgcgtgaac
     8401 aggctgctga gattgtggat atggccctaa gtgaaagtga ccagattctc aaaaatctcc
     8461 acagtaaatc ccaactcaag ccccacgaaa tcattgaagc caaggccaag ttgaaaaaat
     8521 tggctcctga aaaagtggac ttgtctaaaa acaaggtcct tcaaaaggct aagaaaaaac
     8581 gagctccaaa ggtgggagat gatatcgtgg ttctcagtta tggtcagcgt ggtaccttga
     8641 ccagtcaact caaggacggc cgctgggaag cccaagttgg cttgattaag atgaccttgg
     8701 aagagaaaga gtttgatctt gttcaagccc agcaagaaaa accagtcaag aagaaacagg
     8761 tcaatgttgt gaaacgaact tctgggcgag gacctcaagc tagactggat cttcgaggca
     8821 agcgctatga agaagccatg aatgagctag ataccttcat cgaccaagct ttgcttaaca
     8881 atatggctca agttgatatc atccatggta tcggaacagg agtcatccgt gaaggagtta
     8941 ccaaatactt gcaaagaaac aaacatgtca agagtttcgg ctatgcccca caaaatgctg
     9001 gaggcagtgg tgcgactatt gtcactttta aaggatagca gtattctgga ctttataaag
     9061 taaaaactgt tgaactaatt tttactaata aacacattga caaaagccaa cattttttgt
     9121 aaaattagaa tcaattaata ccaacaccga atgaagttta atagaagtgg ggaatcgttt
     9181 gattttccat gactgtaaat ggacggaact ctggagagac cgtaaaggca ccgaaggggc
     9241 aaggcaggca actgctcaaa ctctcaggta aaaggacaga gctaggatag accgcttttt
     9301 agcatttatc taagcattcc agagtacatg tatcttgcat gtgctctttc ttttggggtt
     9361 gaaacgatag gagaaggaaa tgttagaatt gcttaaatca atcgatgatt ttgcttgggg
     9421 accgcccctc ttgattttat tggtcggaac aggggtttac ctaaccatgc ggctaggact
     9481 cttgcaggtt ttgcgtctgc ccaaggcctt tcagcttatt tttatccagg ataagggaca
     9541 tggtgatgta tccagttttg cagctctgtg tacagccttg gcatcaactg ttggaacagg
     9601 aaatatcata ggagttgcga cggctatcaa ggttggtgga ccaggagctc tattttggat
     9661 gtggatggcg gctttctttg gaatggctac caagtatgcg gaaggactct tggccatcaa
     9721 ataccgcacc aaggacgacc atggtgcagt agcgggaggt cccatgcact atatccttct
     9781 agggatggga gaaaagtggc gaccacttgc tgttttgttt gcagtagcag gagtattggt
     9841 tgctctcttg ggaatcggaa ccttcaccca agtcaactcg attacagaat ctatccaaaa
     9901 tacaacgacg atttcgccag ccatcacagc tctcgtcttg tctgtctttg tagcgattgc
     9961 agtctttggt ggactcaagt ctatttctaa ggtttcaact actgttgttc cttttatggc
    10021 catcatttat atcttaggaa ctcttacagt tattttcttt aatatcggaa aaatccctgg
    10081 cacaatcgct ttagtcttta cctcagcttt tagtcccctt gctgcggtag gtggatttgc
    10141 tggtgctagc gttcggatgg ctattcaaaa tggtgtggcg cgtggtgtgt tctcaaacga
    10201 atctggtctg ggttctgctc ctattgcagc tgcagctgcc aagacaaatg aaccagtaga
    10261 gcaaggtttg atttccatga caggaacctt tattgatacc ctcatcattt gtactctaac
    10321 tggtttgacc atcttggtaa ctggagtttg gagtggtgac ttgaatgggg ttgccttgac
    10381 tcagtcagct ttctcaacag tcttttcaca ctttgggcct gccctcttga ccatcttcct
    10441 tgtacttttt gcctttacaa cgattctagg ttggaactat tacggagaac gctgttttga
    10501 gttcctcttt ggggttcgct ttatctggct ctaccgtgtg gtttttgtgc tcatggtctt
    10561 gttaggagga tttatcgagt tggatatggt ctggattatc gcagatatcg ttaacgcctt
    10621 gatggctctg ccaaacttga ttgccctctt ggtcttgtcg ccagtcgtta ttgctgaaac
    10681 taaaaagtat tttgacaaat aatggaatca cacagctcgt gtgatttttt tgcctataaa
    10741 taatttttat cagcctgata tataatatat attatgcaag tgattggaag tgtgataaac
    10801 taatataaca acgaaaatct tatgagagaa taaagcttta tcttagataa acttaggaat
    10861 caggatttta taagattttc caataatatt agtgtcaata ataagaaaag aggtatctta
    10921 tgacaacatt tactatccat acagtagagt cagcaccagc agaagtgaaa gaaattcttg
    10981 aaacagtaga aaaagacaac aatggctata ttcccaacct aatcggtctc ttggccaatg
    11041 ccccgactgt tttagaagcc taccaaattg tctcatctat ccaccgtcgc aacagcctga
    11101 cacccgttga gcgtgaagtg gtgcaaatca cggcagccgt gaccaatggt tgtgccttct
    11161 gtgtcgcagg tcacacagcc ttttccatca aacaaatcca gatgaatgat gacttgattc
    11221 aagctcttcg caatcgtact ccaattgaaa cagatcctaa attggatacc ctagctaagt
    11281 ttaccttggc agttatcaat accaagggtc gtgtaggaga tgaagccttg tctgagtttt
    11341 tagaagctgg ctacactcaa caaaatgcct tggatgtggt ttttggtgtc agcctagcaa
    11401 tcctctgtaa ctatgccaac aacttagcta atacaccaat taatccagaa ttgcaacctt
    11461 atgcctaatt catatctgag taaaatgaag tctgaaataa gcggacttcg tttttttaaa
    11521 tcctcattta agcgctttta tgttatactg aaactaacat gattattgga ggttaggatg
    11581 aaaaaactcc ccttagtatt ttcaggttgt ttgctaggtt tggcaggagc tggaaatctt
    11641 attttagata cgttgccggt tctatcccat ctttttagtc tgactggttt ggttttatgg
    11701 atttactttc taattctgca tctctttaat tggaaagaaa ccaagcaaga attgaccaag
    11761 ccccctcttt tgtcaggaat ggcaaccttt cctatggctg ggatgatttt atcgacctat
    11821 gtctttcgcg tattctctta tcttcctttg gtagcacaag ggatttggtg gttttcattt
    11881 ctcttggatt tgaccttgat tgctggtttt accatcaagt ttgcttgtcc agggcggagg
    11941 gttcatgcca ctccaagctg gacggttctc tatgtgggga tagcagtggc tgccttgacc
    12001 tatcctctgg taggtattat cgaaattgcc tatgcgacct tgagttttgg ttttctcttg
    12061 accttctatc tctatcccct tatttatagc gatttaaaga aacatccact cccactagcc
    12121 ttgcttggac aagaaggaat ctactgtgct cctttctctc tactcttggc ttctctagtt
    12181 cgagtaggag gaaccagcct gccgacttgg gtcttgattg tcatgatttt ggcttctcaa
    12241 tccttctttt tctttgtttt aactcgtctg cccaacattt taaaacaagg ttttcaacca
    12301 gccttctcag ccctcacctt cccaaccatt atcacagcga cctcgctcaa gatggctcag
    12361 ggaattttga aacttccatt tctggattac ctggtattgg ctgaaaccat tatatgccta
    12421 actattttat tctttgtact aggtgcttat ctgatttggt tacgaaaaaa ggtctagcta
    12481 gaaatagcta aaccttattt tttatggttt gatgacttca gctccaccca tgtatggacg
    12541 aagtgcttct gggatggtca cagaaccatc ttcattttgg taattttcaa gaattgcagc
    12601 cactgtacgt ccaactgcaa gtccagaacc gttcaaggta tgaaggagtt tcaccttgcc
    12661 atctgcttca tcacggtaac ggatttgggc acgacgggct tggaaatctt ctgtgtttga
    12721 acagcttgag atttcacggt aattgttttg tgctggaatc cacacttcca agtcgtaagt
    12781 cttcgtagct gagaagccca tatctccagt agagagagca acgacacggt atggaaggtt
    12841 gagtttttga agaatgtttt cagcgttggc tgtcattttt tccaattctt cgtaagattc
    12901 ttctggtttg gcaaatttga ccatttcaac cttgtggaat tggtgcaaac ggatcaagcc
    12961 acgcgtatca cgaccggcag aaccagcctc agaacggaat gacggactca tagcagtgaa
    13021 gtagattgga agatctttgc cgtctaagat ttcatcacgg tagtagtttg tcagaggaac
    13081 ttcagcagtt ggaatcaaga caaagttggt atcgctgagt tcaaaagtat cttccttaaa
    13141 ttttggatac tgaccagtac caaacataga atcatggttg actatgtaag gtgtgatgac
    13201 ttcagtatag ccttcttttc catgttcatc caacataaag ttgtagatag cacgttccaa
    13261 acgagcaccg aggcctttat agaagaggaa gcgagcgcct gttaccttac caccgcgttc
    13321 ccagtcaagg ataccaaggt cttcaccgag atcccagtga gctttaggtt cgaagtcaaa
    13381 ctcgcgtgga gtaccccaac ggcgaacttc cacattgtcg tcttcgtcag ccccaacagg
    13441 aacgctgtca gctgggatat ttggaagagt cgttgtaaat tctgtcaatt tagcatcgat
    13501 ttctgccaat tcagcatcca aggctttaac ctcagcagat agattttgca tggcagcaat
    13561 cttgtcatct gtattttcct tgttgcgctt agcttgggca atctcagcag aaactgtgtt
    13621 acgttctgct ttgagagttt caaccttgat caagatgtta cgacgtttag catcgatttc
    13681 tttcatttca ttcaagacag cagcatctac accacgtgta gctaattttt ctgcgacagc
    13741 ttcaaaatct gtacgaatac gtttgatatc taacataaga actcctttat gaaaaaagca
    13801 cgcctgacaa aacgttggag tggcagggcc acggttccat ccaacttcac aggtgtgcac
    13861 ttgattgtgt atgtaattgt tactaacggt agaatttcac ctatccctcc tatctgctcg
    13921 cagtacccgc agactttctg aaagaagaag ataacctact tatccgttgc tatgattata
    13981 ctaaagtttc tacttttttg caaatagatt tttaaatttt tggctaattg tctgaatcag
    14041 ggtcggaagt ttgacgacct tgtcattgcc tagttttttg cgtgcaattt tgagaatggc
    14101 acctgagtct tttgaagcaa agaggaattt tcctttgtct gtaaagactt cgaagtggcg
    14161 gctgattttg cgtccagtga cattggctcc aatctgattg atatggctcc aaggaatctg
    14221 gataaattgt tcgacattga catctgggta aaattccaaa gcctgatctc cgacaaggaa
    14281 tttcccaact ttcccagcga tagagaggta ggaagtgcct gtcgtactga ggagtactgt
    14341 tttgttaagt gattgagcca tgcttagtct tccttacttt ctccaaaaaa ggcattgtag
    14401 agggctttaa ttgctgcttt ctcttggtct ttattgacaa caaacataat agaaacttca
    14461 ctagaacctt gagacatcat ctggatgttg attttgtttt cagatagagc gcgtgtcgca
    14521 gtagcagtca ctccgatatg gctcttcatt ttttcaccaa caatcataat gatagaaagg
    14581 tcgtgttcga tttctgcatg atctacttta gccttttgaa ccaactgacg caggatttct
    14641 tcttccttga tgggagttag ttggcgagaa cggagaatga tagaaagatc gtcgatacct
    14701 gttggcatat gttcccaacc gatgttaagt tcttccagga tttgcagaac cttgcggcca
    14761 aatccaacct cacggttcat gaggtatttc gacatgttaa tgctgacaaa gcctgagtca
    14821 ccagcaattc ccacaactgg aaattcatca ttactgtgtt ttagaacgat acgagtacct
    14881 ggatggtcag ggttgttggt attcttgata accagaggaa tttttccacg gtaggcagga
    14941 agaagagcct cgtcatgaag gactgagaag cctgcatagg ccaactcgcg catttcacgg
    15001 taggtcaact caggaatcga gtgtggttgg tggataatac cagggtgagc tgcaaagata
    15061 ccatcaacgt ccgtaaagtt ttcatagagg tcagctttga caccagcagc aatgatagaa
    15121 cctgtaatat cagaacctcc acgtgagaaa gtacagattt gattttcctt agtgacacca
    15181 aagaaaccag gaatgacaag gacttcattg gtgtttgtca attcttcaat cttgtcataa
    15241 cttgatggaa tgatgcgagc gtgaccaggt tcacttgtga ccacaatccc agcttctcta
    15301 gggtgcatat agcgtgcatc gataccattt tggttaaagt aggcagcaat caatttggca
    15361 ttgttatttt caccggctgc taggaaagta tcgtagagaa attcattttc ttcaatagga
    15421 agagtggcca aggcgtgaat acttttagaa attttttcta gcacagctgg ttttagtccc
    15481 aattcactaa ccatagcagc atagcggtcg ataatccagc tttggctctt gctaatatcg
    15541 ttacccgcaa catagtcgcg gtagtattta atcagggcat ccgtaacctt agtatcttca
    15601 gcattgcgtt taccaggcgc agaaacgact acaaaacgac gctctgaatc acttttgacg
    15661 atgtttaaaa ctttttctaa ttgactagca gaggcaagag aactacctcc aaatttaaca
    15721 actttcataa gaactcctaa agtaagtatt ttatacgatt atagcagaaa gaaaggcttt
    15781 tttcaatgaa gaaaataaga tgctttctag cctaaagcga gcctttctta tcggctgaga
    15841 cactttcagt cgattttttc ttgctttctg ctcctaaaaa agatatactt tgacagtgaa
    15901 ataatttaac actcaatgaa atcaaagagc aaactaggaa gctagccgca gactgctcaa
    15961 agcactgctt tgaggttgta aataagactg acgaagtcag ctcaaaatac tgttttgagg
    16021 ttgcagatgg aagctgacgc ggtttgaaga gattttcgaa gagtataaac tgcttataaa
    16081 ataaaaagga gccctgatgg aacacattat ttatcagctt gaagaggact tggcaatcct
    16141 taccttgaac cgtcctgagg tcgcaaatgg ttttcatatt cccatgtgtg aggagatttt
    16201 agaagctctg actttggcag aagaaaatcc agctgtgcat tttatcttaa tcaatgccaa
    16261 tggaaaggtc ttctcagttg ggggagattt ggtagagatg aagcgggcag tggatgagga
    16321 tgatattcca tcattgacaa aaatcgcaga gttggtcaat acgatttctt ataaaatcaa
    16381 gcaaatagcc aaacctgttt taatggaagt tgacggtgct gttgcaggtg ccgcagcgaa
    16441 tatggctgtt gcggcagatt tctgtttggc gacggataag gctaagttta tccaagcctt
    16501 tgttggtgtt ggtttggctc cagatgcagg gggaattcat ctcttgagcc gcagtatcgg
    16561 tgtgacgcgt gctgctcaat tagctatgac aggagaggct ttaacggcag aaaaagccct
    16621 agaatggggg ttggtttacc gcgtttctga agctgaaaaa cttgaaaaga cgagagaaca
    16681 gcttcttaaa aaattaagac gtgcttcaag taattcctat gctgccatta agaagttggt
    16741 ttgggagagc caatttaaag actggcaggg ttacgctact ttagaactga acctacagaa
    16801 atccttagct caaacagagg atttcaaaga aggagtgcga gctcattcgg agagaagaag
    16861 acctaaattt acaggaaaat aaaaaatact tgcatcattc tttgaatttt gatataattc
    16921 tttctgtcaa atgttttgat tgtaaaagtt ttttgaagaa ggagggatat gaaaaatgga
    16981 ctaccaacga atcaatgaat atttaacatc tatatttaac aatgtccttg taattgagga
    17041 agtgaacttg agaggtagtc gttttaagga tatctccatc aaagaaatgc atacgattga
    17101 tgtcatcggt aaggctccag atgtgactcc aagtcaagtg tcaaaagagt tgatggtaac
    17161 tcttggaact gttacgacaa gtttgaacaa tttagagcgt aagggctaca ttgagcgagt
    17221 tcggtcagaa caggatcgtc gtgtggtgca tctgcatttg acaaagaagg gtcgcttgat
    17281 tcatagactg cataaacgct tccacaaggc catggtagaa aaaattattg atggcatgag
    17341 cgaggaagaa attgctgtca tgggtaaagg cttgactaat ctttaccaat ttttggagga
    17401 tttgaaataa tggcttttgc aaaaataagt caggttgctc attatgtgcc agagcaagtg
    17461 gttacaaatc acgacttggc tcagattatg gataccaatg atgagtggat ttcaagtcga
    17521 acgggaatac gacaaaggca tatttcaaga acagaatcta ccagtgattt ggctacagag
    17581 gttgctaaga aactgatggc aaaagctgga ataacaggaa aagaactgga ttttatcatc
    17641 ctagctacca ttactccaga ttcgatgatg ccctctacag ctgctcgtgt tcaagctaat
    17701 attggcgcta ataaagcctt tgcttttgac ttaaccgcgg cttgcagtgg atttgtattt
    17761 gctctttcaa ctgctgaaaa gtttatcgct tctggtcgct ttcaaaaagg cttggtgatt
    17821 ggtagtgaaa ccctctctaa ggcagtcgat tggtcggatc gatcaacagc tgtgttgttt
    17881 ggagatggtg ctggtggtgt cttgttagaa gctagcgagc aagagcattt cttagctgag
    17941 agtctcaata gcgatggaag tcgcagcgag tgtttaactt atgggcattc aggtttgcat
    18001 tctccatttt cagatcaaga aagtgcagat tcgtttttga agatggatgg acgcacagtc
    18061 tttgattttg ccattcgaga tgtagccaag tctatcaagc agactattga tgaatctcct
    18121 atagaggtga cagacttgga ttatctgcta cttcatcaag ccaatgaccg tattttggat
    18181 aagatggcta gaaaaattgg tgttgaccga gccaaacttc cagccaatat gatggaatat
    18241 ggcaatacca gtgcagccag tatcccgatt ttactttcag agtgtgtaga acaaggtctc
    18301 atccctttag atggtagcca gactgttctt ctatcaggct tcggtggagg cttgacctgg
    18361 ggcacgctca ttcttacaat ttaggtaatc atgtggtgaa cacattgtta taaaaaaact
    18421 atttatttta aaggagtcct atcatggcag tatttgaaaa agtacaagaa attatcgttg
    18481 aagaacttgg aaaagacgca tcagaagtaa cacttgaatc aacttttgat gatttggacg
    18541 cagattcatt ggacttgttc caagtaatct cagaaatcga agatgctttt gatatccaaa
    18601 tcgaagcaga aaatgacttg aaaacagttg gtgacttggt tgcttacgtt gaagagcaag
    18661 caaaataagc agaatattag tagaaggagt agggagaaac ccactccctc ttgtttaggt
    18721 aatagtttga ctgtcaaatt atggtgaaat cgaactatta cgtaagcaag gaaggttgga
    18781 ggcactatga aaacgcgtat tacagaatta ttgaagattg actatcctat tttccaagga
    18841 gggatggcct gggttgctga tggtgatttg gcaggggctg tttccaaggc tggaggatta
    18901 ggaattatcg gtgggggaaa tgccccgaaa gaagttgtca aggccaatat tgataaaatc
    18961 aaatcattga ctgataaacc ctttggggtc aacatcatgc tcttatctcc ctttgtggaa
    19021 gatatcgtgg atctcgttat tgaagaaggt gttaaagttg tcacaacagg agcaggaaat
    19081 ccaagcaagt atatggaacg tttccatgaa gctgggataa tcgttattcc tgttgttcct
    19141 agtgtcgctt tagctaaacg catggaaaaa atcggtgcag acgctgttat tgcagaagga
    19201 atggaagctg gggggcatat cggtaaatta acaaccatga ccttggtgcg acaggtagcc
    19261 acagctatat ctattcctgt tattgctgca ggaggaattg cggatggtga aggtgctgcg
    19321 gctggcttta tgctaggtgc agaggctgta caggtgggga cacggtttgt agttgcaaaa
    19381 gagtcgaatg cccatccaaa ctacaaggag aaaattttaa aagcaaggga tattgatact
    19441 acgatttcag ctcagcactt tggtcatgct gttcgtgcta ttaaaaatca gttgactaga
    19501 gattttgaac tggctgaaaa agatgccttt aagcaggaag atcctgattt agaaatcttt
    19561 gaacaaatgg gagcaggtgc cctagccaaa gcagttgttc acggtgatgt ggatggtggc
    19621 tctgtcatgg caggtcaaat cgcagggctt gtttctaaag aagaaacagc tgaagaaatc
    19681 ctaaaagatt tgtattacgg agccgctaag aaaattcaag aagaagcctc tcgctgggca
    19741 ggagttgtaa gaaatgacta aaacagcctt tttatttgct ggtcaaggtg cccagtatct
    19801 agggatggga cgggatttct atgatcagta tccgattgtc aaagaaacga ttgatcgagc
    19861 gagtcaggtg ctcggttatg atttacgtta tctcatcgat acggaagaag acaaactcaa
    19921 tcagacccgc tatacgcaac cagccattct agcgacttcg gttgctatct accgtttatt
    19981 gcaagaaaag ggctatcagc ctgatatggt tgctggtttg tctcttggag aatactctgc
    20041 cttggtggca agcggcgcct tggattttga agatgcggtt gccttggtag ctaagcgtgg
    20101 agcctatatg gaagaagcgg ctcctgctga ctctggcaag atggtagcag ttctcaatac
    20161 gccagtagag gtcattgaag aagcctgtca aaaagcttct gaacttggag tggttactcc
    20221 agccaactat aacacacctg cacaaatcgt cattgctgga gaagtggttg cagttgatcg
    20281 agcggttgaa cttttgcaag aagcaggtgc caaacgcttg attcctctta aggtgtcagg
    20341 tccctttcac accgctctcc ttgagcctgc tagccagaaa ctagctgaaa ctctagctca
    20401 ggtaagtttt tcagatttta cttgtcccct agtcggcaat acagaagctg ctgtgatgca
    20461 aaaagaggac attgctcagc tcttgacgcg tcaggtcaag gaacccgttc gtttctatga
    20521 aagtattggg gtcatgcaag aagcaggcat aagcaacttt atcgagattg gaccggggaa
    20581 agtcttgtca ggttttgtta aaaaaattga tcaaactgct cacttagctc atgtggaaga
    20641 tcaagcgagt ttagtagcac ttttagaaaa atagactaaa ataagtagaa gttttgaaag
    20701 gaaaaaaatg aaactagaac ataaaaatat ctttattaca ggttcgagtc gtggaattgg
    20761 tcttgccatc gcccacaagt ttgctcaagc aggagccaac attgtcttaa acagtcgtgg
    20821 ggcaatctca gaagaattgc tcgctgagtt ttcaaactat ggtatcaagg tggttcccat
    20881 ttcaggagat gtatcagatt ttgcagacgc taagcgtatg attgatcaag ctattgcaga
    20941 actgggttca gtagatgttt tggtcaacaa tgcagggatt acccaagata ctcttatgct
    21001 caagatgaca gaagcagatt ttgaaaaagt gctcaaggtc aatctgactg gtgcctttaa
    21061 tatgacacaa tcagtcttga aaccgatgat gaaagccaga gaaggtgcta tcattaatat
    21121 gtctagtgtt gttggtttga tggggaatat tggtcaagct aactatgctg cttctaaggc
    21181 tggcttgatt ggctttacca agtctgtggc acgcgaggtc gctagtcgga atatacgagt
    21241 caatgtgatt gctccaggaa tgattgagtc tgatatgaca gctatcttat cagataagat
    21301 taaggaagct acactagctc agattccgat gaaagaattt gggcaggcag agcaggttgc
    21361 agatttgaca gtatttttag caggccaaga ttatctaact ggtcaagtgg ttgccattga
    21421 tggtggctta agtatgtagc gaaagctaga ggtgaaaaga atgaaactaa atcgagtagt
    21481 ggtaacaggt tatggagtaa catctccaat cggaaataca ccagaagaat tttggaatag
    21541 tttagcaact gggaaaatcg gcattggtgg cattacaaaa tttgatcata gtgactttga
    21601 tgtgcataat gcggcagaaa tccaagattt tccgttcgat aaatactttg taaaaaaaga
    21661 taccaaccgt tttgataact attctttata tgccttgtat gcagcccaag aggctgtaaa
    21721 tcatgccaat cttgatgtag aggctcttaa tagggatcgt tttggtgtta tcgttgcatc
    21781 tggtattggt ggaatcaagg aaattgaaga tcaggtactt cgccttcatg aaaaaggacc
    21841 caaacgtgtc aaaccaatga ctcttccaaa agctttacca aatatggctt ctgggaatgt
    21901 agccatgcgt tttggtgcaa acggtgtttg taaatctatc aatactgcct gctcttcatc
    21961 aaatgatgcg attggggatg ccttccgctc cattaagttt ggtttccaag atgtgatgtt
    22021 ggtgggagga acagaagctt ctatcacacc ttttgccatc gctggattcc aagccttaac
    22081 agctctctct actacagagg atccaactcg tgcttcgatc ccatttgata aggatcgcaa
    22141 tgggtttgtt atgggtgaag gttcagggat gttggttcta gaaagtcttg aacacgctga
    22201 aaaacgtgga gctactatcc tggctgaagt ggttggttac ggaaatactt gtgatgccta
    22261 ccacatgact tctccacatc cagaaggtca gggagctatc aaggccatca aactagcctt
    22321 ggaagaagct gagatttctc cagagcaagt agcctatgtc aatgctcacg gaacgtcaac
    22381 tcctgccaat gaaaaaggag aaagtggtgc tatcgtagct gttcttggta aggaagtacc
    22441 tgtatcatca accaagtctt ttacaggaca tttgctgggg gctgcgggtg cagtagaagc
    22501 tatcgtcacc atcgaagcta tgcgtcataa ctttgtacca atgacagctg ggacaagtga
    22561 agtatcagat tatatcgaag ctaatgtcgt ttatggacaa ggcttggaga aagaaattcc
    22621 atacgctatt tcaaatactt ttggttttgg aggccacaat gcagttcttg ctttcaaacg
    22681 ttgggagaat agataagtat gaatttaaac gatattaaag acttgatgac tcaatttgac
    22741 cagtcaagtt tgagagaatt ttcttataaa aatgggacgg atgagttgca gtttagcaag
    22801 aatgaagcga gacctgtgcc tgaagttgca actcaagtcg ctccagcacc cgttctagca
    22861 acaccgagtc cagtagctcc tacatctgct ccagcagaga ctgtagcaga agaagttcca
    22921 gctccagctg aagcaagtgt ggctagtgag ggaaatcttg tagagagtcc acttgttgga
    22981 gtggtttact tggctgctgg accagataaa cctgccttcg ttacagttgg tgatagtgtc
    23041 aaaaaaggtc aaacattggt aattatcgaa gccatgaaag tcatgaatga aatcccagct
    23101 cctaaggatg gtgtggtaac ggaaattctc gtctctaacg aagaaatggt tgagtttggt
    23161 aaaggattgg tacgtatcaa atgatcgata ttcaaggaat caaagaagct cttccccacc
    23221 gttatcctat gcttctagtg gaccgtgtct tggaagtgag cgaggatacc attgttgcta
    23281 tcaaaaatgt gaccattaac gaacctttct ttaacggcca ctttcctcaa tacccagtta
    23341 tgccaggtgt tctgattatg gaagccttgg cgcaaactgc tggtgtgttg gagttatcaa
    23401 aacctgaaaa taaaggaaaa ctggtctttt acgctggtat ggacaaggtt aagttcaaga
    23461 agcaagttgt accaggcgac caattggtta tgacagcgac ttttgtaaaa cgtcgtggca
    23521 ccatagctgt ggttgaagca aaggctgaag tggatggcaa gcttgcagcc agtggtatcc
    23581 ttacttttgc aattgggaac taaggaggtt ctccatgttt cgaaaaattt taattgccaa
    23641 tcgtggtgaa attgcggttc gtattatccg tgcggcacgt gaattgggga ttgcgacggt
    23701 agcggtttat tcaactgctg ataaggaagc tcttcatacg cttttggcag atgaagcagt
    23761 ttgtattggt cctggcaagg caacagagtc ttatctcaat attaatgcag ttctatcagc
    23821 tgcagtcttg actgaggcag aagctattca ccctggtttt ggatttctca gtgaaaattc
    23881 caaatttgcg accatgtgtg aagaagtagg tatcaagttt atcggtccat ctggtcatgt
    23941 tatggatatg atgggggata agatcaatgc gcgtgctcag atgattaaag caggtgtgcc
    24001 tgttatacca ggttcagatg gagaagtgca taactctgaa gaagctttga ttgttgctga
    24061 aaaaattggc tatcctgtta tgctcaaggc ttcagcaggt ggaggtggta aagggattcg
    24121 taaggttgaa aaaccagatg acctcgtttc tgcctttgaa actgcctcta gtgaggccaa
    24181 ggccaattat ggcaatggtg ccatgtacat agaacgggtt atctatccag ctcggcacat
    24241 tgaggttcaa atcctaggtg atgagcatgg acatgtgatt cacttgggtg agcgggattg
    24301 ttctcttcaa aggaataacc aaaaggtttt ggaagaaagt ccctcgattg caatcggaaa
    24361 aacgctgcgt catgaaatag gtgctgctgc tgttcgagcg gcagagtttg ttggctatga
    24421 gaatgcagga accattgaat ttcttcttga tgaagcaagt agcaatttct atttcatgga
    24481 gatgaatact cgtgttcagg tagaacatcc agtaacagag tttgtttcag gtgttgatat
    24541 cgttaaggaa cagatttgca ttgcggcagg tcagcctttg tctgttaagc aagaagatat
    24601 tgtcctacgc ggtcatgcca tcgagtgtcg tatcaatgca gaaaacccag cctttaactt
    24661 tgctccaagt ccaggtaaga ttactaatct ctatctgcca agtggtggag ttggcttgcg
    24721 cgtggattca gcagtttatc caggttatac cattccgcct tattatgata gtatgattgc
    24781 caaaatcata gtacacggcg aaaatcgttt tgacgccttg atgaaaatgc aacgtgccct
    24841 ctatgaatta gagattgaag gagtgcagac caatgcagat ttccagcttg acctcatttc
    24901 agatcgcaat gtcattgctg gggattatga tacttccttc ttgatggaaa ccttcttacc
    24961 taaatatcaa gaaaaagaat aaaatgactt taagagttta aaccgaaaag gggaatcaat
    25021 ggctctattt agtaaaaaag ataagtatat tcgaatcaat cccaatcgtt cggttaggga
    25081 aaaacctcaa gctaagccag aggttccaga tgaattattt tcccagtgtc caggctgtaa
    25141 gcataccatc tatcagaagg atctgggaag tgaacgtatc tgtccgcact gtagctatac
    25201 ctttcgtatt tctgcccaag aacgcttggc tttgacgatt gatatgggaa ccttcaaaga
    25261 attgtttaca gggattgaaa gcaaggatcc cttgcatttc cctggttacc aaaagaaact
    25321 ggcatctatg cgtgaaaaaa caggtctgca tgaagccgtt gtgacaggaa ctgctcttat
    25381 taaaggtcag actgtggctc ttgggattat ggattctaac tttatcatgg cttctatggg
    25441 tacggttgta ggtgaaaaaa tcactcgttt gtttgagtat gcgactgtcg aaaaattgcc
    25501 agttgtccta ttcacagcct ctggtggagc ccgtatgcag gaaggaatca tgagtctcat
    25561 gcagatggct aagatctctg cggcggttaa acgtcattca aatgctggtc tcttttacct
    25621 gaccattttg acagatccaa cgactggtgg tgtgacagct tctttcgcta tggaaggcga
    25681 tatcattctg gctgaaccac agagcttggt tggttttgct gggcgtcgtg tgattgaaaa
    25741 tacggttcgt gaaagcttgc ctgaggattt ccaaaaggca gaattcctat tagaacatgg
    25801 ctttgtggat gctattgtca aaagaagaga cttaccagat acgattgcta gcctagtcag
    25861 attgcatgga gggagtccta gatgaatatt gcaaaaatag tcagagaagc gcgtgagcag
    25921 agtcgcttga caaccttgga ctttgcgaca ggcatttttg atgaatttat ccaattacat
    25981 ggtgaccgtt cttttcgtga tgatggtgca gttgttggtg gtattggttg gcttggagac
    26041 caagctgtaa cagttgttgg tatccaaaaa ggcaagagtt tgcaagacaa cctcaaacgg
    26101 aattttggcc aaccacatcc agaaggctac cgaaaggcac tgcggttgat gaaacaggct
    26161 gagaaatttg gccgtccagt tgtgaccttt attaatacag caggtgctta tcctggtgtc
    26221 ggagcagaag aacgtggtca aggggaagct atcgctcgca atctcatgga aatgagtgac
    26281 ctgaaagttc ctattatcgc cattattatc ggtgaaggtg gttcaggcgg ggctctggct
    26341 ctagctgtcg cggaccgtgt ctggatgctg gaaaattcta tctatgccat tctcagtcca
    26401 gaaggctttg cttccatttt atggaaggac ggtactcgcg ccatggaagc agcagaactg
    26461 atgaaaatca cttcgcatga actgttagaa atggacgtgg tggataaggt gatttctgaa
    26521 ataggacttt ctagtaaaga actgattaag agtgtcaaaa aagaactcca aacggagctg
    26581 gctagacttt cacaaaaacc gctagaagag ttgctggaag aacgctatca acgatttaga
    26641 aaatactaag aatgactaaa tgagactaga aaggtctcat tttttatgga aaaatgtaat
    26701 tcaggtcgaa aaattgccag ttgaggagat gggctataaa atagacaaaa aaagaaatat
    26761 actataaatg tgagcctaca acaatctatt ttaagaaggt acttgcaatg acaggtacaa
    26821 atacatttac agttctttca actgaggact tggagcaaac ttcaggtggt cttgctgttt
    26881 gggaagatgg atatagtaga tggttatatt atagagaatt tgctccctat atgaggcaag
    26941 gggcacttaa ttcttatata gatgcttgga agtacggctt ccgagcaggg taatctttca
    27001 aaaaagtacg atgagaaaca caaaaaaact aagacaattt ggaatatttt tgcttattat
    27061 tttactatct acctatttac cacaaacaat tggattgtat gtgacgatca ttttagggtt
    27121 aggagctgat gtctattctc ttattttaac aatgggatta gtgggaagtt tccttctatt
    27181 aatttggagg ttaaaaaaga aaaagatgct ctttatcttt gagaaaaaga gctggaactg
    27241 gtcatttgtc ttctacttat ttgcaactta tgtggtttat cagattctcg gtaatttttg
    27301 ggcacgctat gctcatttga ttaatcatag gaatattcat gatgagtatt ttaccgtgct
    27361 tttttcaaat ggacaaccaa cctttttatc gactatctta tcttttgtcc tgcctgtaat
    27421 catcggtcct gtttttgagg agactcttga tagagggtat tttatgaata ccttctttcc
    27481 ctagtccaag tactatttgg atgttatcct atctgctatc atctttggga ttagccattt
    27541 gatactatct cacagagatc caattagttt attatattat agtctgattg gtttcttttt
    27601 tgcccttgtt tatcgttcta ccgacaatct gagattaacg attctctgcc atagtttttt
    27661 taactttctc aatcatgcaa aacctatctg gatttttgtt tacaattata tctattatca
    27721 tttttttaga tagtgggagg caaagaaaaa agtgtttgat atctatcaaa cactttttct
    27781 ttttattgtt cttctgttac aaactggctg agcagtccat tgataaaacg ggcagatttt
    27841 tgatcggaga agtcctttgc aagctcgata gcttcattaa cagcaaccag ctgaggagtg
    27901 tcaaatgaag tgatttcaaa gactcccaag cgaaggaggt ttctctccac gagcgttaag
    27961 cgttcaatgg tccaacctgc ttttaaatgc tgagtgattt gcttatctag ttcttccttt
    28021 ttagcttgaa caccagaaac gaggtctatc aaaaaggctg gaagttgtac atccgtatct
    28081 tcacgatcat gagtataggc gaaacgacaa gcagtttcga catccgtacc gaactcaagg
    28141 ctcatgagag cttgaaaagc gcatttacgg agttggcgtc tagattctaa tagtggacta
    28201 gtcattgagg aagtcctcgt caaatagatc tttcaattct ggttttggtg ttttatctgg
    28261 gacgatacct gcaacgtgaa tattgatagc agcgagttct acatcagcca tattacggac
    28321 ggcatctttg acagctttct ggatagcaac cgctacctta ggaactttta ctccgtactc
    28381 aaggtagaga tagatatctg ctgtgagttc ttcgtccacg tttttaagat aaatgccacg
    28441 gccgagtgaa agttttgaaa gggtatcaga cactgatctg tttgaaaaag agtgaacacc
    28501 ctctaccttt gcagtagcga tagcaatgat tttttcaagt acacgtgggg cgataacgat
    28561 ttcgccaagt tgttcttcaa ttcccataga atgacctctt tctagagatt aggcacgaga
    28621 aacgtaagtt ccttctgcag tgttgataac gagtttttgt cctgcttcga tgaagtctgg
    28681 aacgtttacg acaagtccag tttccatcgt tgctggttta ccagaacctg taacagtagc
    28741 acctttgata gatggttgag tttcagcaac tgtcaactca acagtagtag gaacggtgac
    28801 accgatcact tcagttccgt agaattggat tttcacatca gagttttcaa ggatgtaaag
    28861 caattcgttt tcaacattga ctacagggat ttcgtattgg tcgtaagttt ctgtattcat
    28921 gaagtatgct gtgtcatcca ttttgtacaa gtattgagct gggacagtct cgataatagc
    28981 ttgttcaaat ttttcctctg gacggtagct tgtgtcaaat gtagaaccag tacggacatc
    29041 acgcaatttc atacgcatga tcgtgtttcc tttacctggt ttgtggtgac tagcttccaa
    29101 aacgcgaatc aatttgccgt cagctgtttc aaaggtcata ccagctttta atttacttgc
    29161 ttcaatcatg tttttacctc tttaataaat attattactc ttcattttac cataaaatac
    29221 tctggaaata aagccaaaat agttattggg agttggtaga gaagaaaaaa ccaaccttag
    29281 ggctggtttt gttctgtcta gttttctttc aacttcgcca attcctgtgc aagtagttta
    29341 agggcaactt gtgggttggc ttggcctttg gttgccttca taaggaatcc tgtaaaggcc
    29401 ttgtcggcgt tacgtttgcc tgacttgaag tcgacaacag cagcttcgtt atcggcaaag
    29461 acttggtgga tgattgggat caagatagct ggatctgaaa tttgaaccat acctgctttt
    29521 tccacgtatt cacgcgcgcc accgccattt ttagctagat ggacaaagac tttcttggca
    29581 atcttagatg aaatagtacc gtcttcgatg atggcaatca tttcaaccaa gttttctggt
    29641 gtcaattcga tttgttccag tgttttacct tcagcattca agaactgagc gacttcccct
    29701 tggagccagt tagagacttg tttggcatca ccatctaggg caacagcttt ttcaaagaaa
    29761 tcagaagtga ctttattagc agtcaactga ctagcatcgt agtctgataa accaaggtca
    29821 gatacataac gcgcacgacg ttcttttgga aactctggca actcagtccg catttcctca
    29881 atccactcgt cagaaatttc aaagaggggt aggtctggtt ctgggaagta gcggtagtct
    29941 gcagcccctt ccttgacacg catgagaatg gttgctttat tcgcttcatc gtaacggcgt
    30001 gtttcttggc ggatttgacc acctgagcga agaatttcag cctggcgttg gacttcgtat
    30061 tcaagacctt tacgaacgtt tgagaaggag ttgaggttct tcaattcagt cttggtaccg
    30121 aatttttctt gaccataagg gcgaagggag atgttggcat ccacacgcat agaaccttct
    30181 tccatcttaa cgtcagaaat gccagcgtac tggataactt ccttgagggc tgtcagataa
    30241 gcataggctt cttcaggaga acgcatatct gcctcagata caatctcaat caagggaacc
    30301 ccttggcggt tgaggtcaac ataagagtag ccgtctgtac catgggtgtt tttaccagcg
    30361 tcttcctcta ggtgggcacg ttcgataccg attttcttgg tcgtaccgtc ttctagttcg
    30421 acttcaatcc agccattata tccgattggt tcatcaaatt gagaaatttg gtaggctttg
    30481 gggttatcag gatagaagta gttcttgcgg tcaaagtgca tctttttgtg gatgtccatg
    30541 ttgagggcaa gagcagcctt gattccggca tcgacaaccc ctttattgag aactggcaga
    30601 actcctggga aagaccagtc aatcacgtta gtgttggcat tttgatcatt tccgaagtgg
    30661 gcagaagtag gtgagaagat ttttgaattg gtgttgagct ctacgtggac ttcaagtccg
    30721 atgactgttt caaagttcat tagttgtcac ctccaaaaat cacgggttgt tgtttgtggt
    30781 agtctgttgt tgcttcaaaa gcagcagcag cttggtaaat ggtttcctca gagtacttgg
    30841 gaccaatcaa ttgcagaccg acaggtagac cttgagagaa tccagcagga atcgaaattc
    30901 cagggagacc tgctaagttt acaggtatgg tcaagaggtc agccaagtac atggcaactg
    30961 ggtcatggtt gagtgagtcc aagtcatagg caacacttgg agcagttgga cccaaaatca
    31021 aatcgtaatc cgcgaagact ttttcgaaat cttgaatgat gagggtacgg acttgaccag
    31081 cctttttgta gtaggcatca tagtaacctg atgaaagact gaaagtaccc agcatgatac
    31141 gacgttttac ctcttcacca aaaccttggc ttcggctgtt tacatagatt tcatcaaggt
    31201 tggttgcatc ttctgcgcga tagccgtaac ggataccgtc gaagcgttgc aagtttgatg
    31261 aagcttctga tgaagcgatg atgtagtata cggcaactcc gtatttagag tgaggaaggc
    31321 tgacttcttc gacaatagca cccaattttt caaagtgttt agcggcattt agaatggttt
    31381 ccttaacctc tgggttaatt ccttcaccga ggtattcctt aggcaaagcg attttcatac
    31441 ccttgatgtc ttggccgatt tttgaagtaa agtcggcgat gcggacagga gcagaagtag
    31501 agtctttagc atcttcgctg gcaatagcgt tgagcaagag ggcattttcc ttaacagtag
    31561 gagcaaaagg tccaatctgg tctaatgagc taccaaaggc aatgagaccg aaacgtgaaa
    31621 ctgttccgta ggttggtttg agaccaacga ttccgttgaa ggcagcaggt tggcggatgg
    31681 aaccaccagt atcagaacca agtgacaagc gaacttgtcc tgaggctaca gctgcggcag
    31741 aaccacttga tgacccacca ggaaccttgc tgtggtccca agcgttttta gttgctccgt
    31801 agtgtgaagt ttcacctgaa ccacccatag caaattcgtc catgttggtc tttccaacga
    31861 caatcatgcc cttggttttt gcattggcaa cagctgtcgc atcaaagatt ggctcatagt
    31921 tgtagagcat ttttgaggca gcagttgtga gaataccgtc tgtagagatg ttatccttaa
    31981 cagcaagtgg aattcctgaa aggacattgt cagcatcaat tccagcttca tcaatggctt
    32041 tagcttgaac aagagcttgc tcctcagcga tggtgacaaa tgaattgagg gcttcctcac
    32101 gagacttgat attttcaagt gttgcttggg tcaattctgt tgcagaaatt tccttagaga
    32161 caaggagatt gtgcaactct tcaatagttt tattgttaaa agtcattagg catctcctcc
    32221 atcgtctagg atagctggca ccttgatata gtagttgtct tttttaggta cgtttttaaa
    32281 caagcgatca cggtctgttc cttcttcggc cacatcaggg cggagtacag tcttgcggtc
    32341 agccatagtc gtagtaggtg cgacaccagt tgtgtcaact tcgcccagca attcaaccat
    32401 gtcaacaatc ttagacaagg tggtcgcaaa ggcagcagtt tctttttcag agaatcttaa
    32461 ttttgaaaga ttggcaacgt gtgttacctc ttcttgcgta attttcatct tgcatccttt
    32521 cgtgaaatga tgatttttag tctgttctat tttaccatat tttcctataa ataagggagg
    32581 gggaagtgaa aagaaataga aattgaaaaa aatgctaaaa tccgatataa tggagtgttg
    32641 aaaggaatgg taaaatccaa tgtaaaaatc attctcagct attgaaacaa gaaaaataga
    32701 gaatcaaaga aagagaactt atgaatattc aagaagaaat taagaaacgt cgtacctttg
    32761 ccattatctc ccacccggac gcggggaaaa caaccatcac tgagcagtta ctctactttg
    32821 ggggtgagat tcgtgaggct ggtacggtaa aagggaagaa aacagggact tttgctaaat
    32881 ctgactggat ggatatcgag aagcaacgtg ggatttctgt tacttcatct gttatgcaat
    32941 ttgactacga cggcaagcgc gtgaatatct tagacacgcc agggcacgag gacttctcag
    33001 aagataccta tcgtaccttg atggcggtgg atgctgcggt catggtcgtg gactctgcca
    33061 aggggatcga ggctcaaacc aagaaattgt ttgaggttgt gaaacatcgt ggcattccag
    33121 tctttacctt tatgaacaag ctggaccgtg acggtcgtga gcctttggat ctcttgcaag
    33181 aattggaaga aatcttgggc attgctagct accctatgaa ctggcctatc gggatgggga
    33241 aagcctttga gggcttgtat gacctctata accaacgttt agagctttac aaaggggatg
    33301 agcgttttgc cagtctagaa gacggggaca aactctttgg tagcaatcct ttctacgagc
    33361 aagtcaagga tgacattgag cttttaaatg aagccgggaa tgagttttca gaggaagcta
    33421 ttctggctgg agaattgacg cctgtctttt tcggttcagc cctgacaaac tttggtgtgc
    33481 agaccttcct tgaaaccttc ctcaagtttg ctccagaacc acatggtcac aagaaaacag
    33541 acggtgaaat tgtggatcct tatgacaagg atttctcagg atttgtcttt aaaatccaag
    33601 ccaacatgga ccctcgtcac cgtgaccgta ttgcctttgt ccgtatcgta tctggcgaat
    33661 ttgagcgcgg catgagtgtc aatctccctc gtactggtaa gggggctaaa ctgtctaatg
    33721 ttactcagtt tatggcggaa agtcgtgaga atgtgatcaa tgccgtggca ggtgatatta
    33781 tcggggttta cgataccggt acttatcaag ttggggatac cttgacagtt ggtaaaaaca
    33841 agtttgaatt tgaaccactg ccaaccttta ctcctgaaat tttcatgaaa gtttctgcta
    33901 agaatgttat gaagcaaaaa tccttccaca aggggattga gcaattggtg caagaaggtg
    33961 ctattcagct ttataagaat taccaaacag gtgagtacat gctaggcgct gtaggtcaac
    34021 tccagtttga agtctttaaa caccgcatgg aaggtgagta caatgcagaa gtagttatga
    34081 acccaatggg taaaaagacc gttcgttgga tcaagcctga ggacttggat gaacggatgt
    34141 cgtcaagtcg caatatctta gccaaagacc gttttgacca accagtcttt ctctttgaaa
    34201 atgactttgc cctccgctgg tttgcggaca agtatccaga cgtagagtta gaggagaaga
    34261 tgtgactcag taccaacaat tggaactaaa gttccaaatt gttggacgct agtcgctatt
    34321 tggcgaacta gctaactgcc ttactaacta cgtttggcaa atgaaatcga tttgtaaaac
    34381 tccgtgtcgt aatgtaaaaa ttgtaaaccg taatgggact tcatctaaaa tctccactgg
    34441 tatggtttta cgaacgaatg ggcgaaaata atggttacgt agaaacatat gatgggaata
    34501 aaaagaaatg agataaagag tggaagaaga gaaaaaagcg attgtcttag gtgcagataa
    34561 tgcttatatg gataaagttg aaataacaat aaaatctctt tgtgttcatc tattataatc
    34621 ttaagtttta tgtttttaat gatgaccttc cgcgagaatg gttccaattg atggaaaaaa
    34681 gactagagac tttgaatagt gagattgtga atgtttagat tgacagtagt attctgaaag
    34741 gttatcgtct cccctttgag ggactttcat atgatgattt ttatacacat aattttaatt
    34801 ctggtgttct gttaatcaac aatggcatgt ggagagcaga aaatgtcacg caagacctga
    34861 tttaagacac aaataaaaaa tcttccaatt cccttgcaaa aaacaagaaa gtgtgataac
    34921 atagtacggt atgtggtgct agcacatccg ctatattaga tctaatagga ggaaaacaca
    34981 atggctaaag tatgttactt tacaggtcgt aagactgtat caggaaacaa ccgttcacac
    35041 gcgatgaacc aaacaaaacg tgccgtaaaa ccaaaccttc aaaaagttac tgttcttatc
    35101 gatggtaaac ctaaaaaagt ttgggcttca gctcgtgctt tgaaatcagg taaagttgaa
    35161 cgcgtttaat aataaaagca aaggagacct tagggtcttt tttcttttat tgtgggtata
    35221 acatcgtttg aaaaatagag taaatatccg ctgatacatc attctgcttt tacacttggg
    35281 ctgaaatatg ataaaataga gtatcaacta gttgaggtaa aaaggatgac tgtaaaaatt
    35341 aatacaaaag atggtcaaat cgaactaaca gatgaagtga ttgcaaccgt cgtaggtgga
    35401 gcagcaactg agatttttgg tgtggtcggt atggctagta aaaatgccct caaagataat
    35461 ttccaagccc tgctaggtaa ggaaaattat tctaaaggtg tcgtcgtaaa ggcagccgaa
    35521 gatggcagta ttgcagttga tgtatatacc gtgttaagct acggaacaaa gattagtgaa
    35581 gtgtcaaaaa acattcaaga gcgtgttgcc tttagcttgg aaaaccagct tggaattact
    35641 gctcagactg taaatgtcta cattcaaaat atcaaagttg taggagaata accgtgtcaa
    35701 aaattactac tagcttattt caagaaatgg tgcaggctgc atcaactcgc ttgaataagc
    35761 aagctgaata tgtcaattca ttaaacgtct ttccagttcc agatggagat actgggacaa
    35821 atatgggaat gaccattgaa aatggtgcta aagaagttgc agacaagcca gcttctacag
    35881 ttggagaagt agcgagcatt cttgccaaag ggcttttgat gggtgcgcgt gggaactcag
    35941 gagtgattac atctcagctt ttccgtggat tttcccaagc tatcaaggat aaagacgagt
    36001 taacaggtca agacttggct ctggccttcc aatcaggtgt ggaagttgcc tataaggcag
    36061 tgatgaaacc tgttgaagga acgattttga cagtttctcg tggagctgct atcggtgcta
    36121 agaaaaaagc tgagcaaaca gatgacactg ttgaagtcat gcgcgcagcc ttggaaggtg
    36181 ctaaaacagc tctagctaaa acaccagaca tgcttccagt attgaaagaa gttggcgttg
    36241 tggactcagg tggtcaagga ttggtcttca tctacgaagg tttcctttca gcccttactg
    36301 gcgaatatat tgcatctgag gactttgtag cgactcctgc taacatgagt gagatgatca
    36361 atgtagagca tcataagtct gtagctggtc acgtagcgac tgaggacatc acgtttggtt
    36421 actgtactga aatcatggta gctcttaagc aaggtccaac ttatgctaaa gattttgact
    36481 acgatgaatt ccgtaactac ttggatgagc ttggggattc tctcctcgtt gttaacgatg
    36541 atgaaattgt caaagtccat gttcatacag aagatccagg acttgttatg caagaaggtc
    36601 tcaaatatgg tagcttggtc aaggtaaaag ttgacaatat gcgtaaccaa cacgaagcac
    36661 aggttgagaa agaagctact caagttatca agtcggctga agaaaaagag tatgctttga
    36721 ttgctgtggt ggctggtaaa ggtctagcag atatcttctg ttctcaaggc gtggattatg
    36781 ttatcgaagg cggtcaaacc atgaaccctt caacagaaga ctttatcaag gctgttgaac
    36841 aggtcaatgc ccgtaacatc atcttcttgc caaacaacaa gaacatcttc atggcagctc
    36901 aatctgcggc agaagttttg gagcaaccag cagtagtggt agaggctcgc actcttcctc
    36961 aaggtatgac aagtcttctt gcctttgatc caagcaagtc cattgaagaa aatcaagagc
    37021 gtatgacagc tgctcttagc gatgtcgtta gcggaagcgt cacaacagcc gtgcgtgata
    37081 caacgatcga tggcttagaa atccatgaaa acgataatct aggtatggtg gatggaaaaa
    37141 ttcttgtgtc aaaccctgat atgcaccaaa cattgactga aaccttgaaa catatgttgg
    37201 atgaagacag tgaaatcgta accttctatg tcggtgaaga cggaagcgaa gaacttgcca
    37261 atgaaatcgc tcaagaaatc gtagaagaat tcgaagacgt tgaagtcgag attcaccaag
    37321 gtcaacaacc tgtttaccca tacctattta gtgtggaata agattaatgg gctctttgtc
    37381 aactgtagtg ggttgaaaaa aaagctaagc tcgagaaagg acaaatttcg ttctttcttt
    37441 tttgatattc agggcgataa aaatccgttt tttgaagttt tcaaagttcc gaaaaccaaa
    37501 ggcattgcgc ttgataagtt tgatgagatt attggtcgct tccaatttgg cgttagaata
    37561 gtgtagttga agggcgttga cgattttctc tttgtccttt agaaaggttt taaagacagt
    37621 ctgaaaaaga ggatgaacct gcttaagatt gtcctcaatg agtccgaaaa atttctccgg
    37681 ttccttattc tgaaagtgaa aaagtaagag ttgatagatc tgatagtggt gtttcaagtc
    37741 ttctgaatag cttaaaatct tgtcaagaat ttctttattt gttaagtgca tgcgaaaagt
    37801 agggcgataa aaacgtttat cgctcaattt acgactatcc tgttggatga gtttccagta
    37861 acgtttgata gtcttgtatt catgagattt tcgttcaaac tgattcataa tttgaacacg
    37921 aaaacgactc atggcacggc tgagatgttg gataatatgg aaacgatcta gaacgatttt
    37981 agcacacgga aaaagctgtt tagccaagtc atagtaagga ctaaacatat ccatcgtaat
    38041 gattttcact tgacaacgaa cggctctatc gtagcgaaga aagtgatttc ggatgacagc
    38101 ttgtgttctg ccttcaagaa cagtgataat attaagatta tcaaaatctt gcgcaatgaa
    38161 actcatcttt cccttagtga aggcatactc atcccaagac ataatctttg gaagccgaga
    38221 aaaatcatgc tcaaagtgaa agtcattgag cttgcgaatg acagttgaag ttgaaatggc
    38281 cagctgatgg gcaatatcag tcatagaaat tttttcaatt aacttttgag caattttttg
    38341 gttgatgata cgagggattt ggtgattttt ctttaccagg ggagtctcag caaccatcat
    38401 ttttgaagag tgatagcact tgaaacggcg ttttctaagg agaattctag aaggcatacc
    38461 agttgtttcg aggtaaggga tcttagacgg tttttgaaag tcatatttct tcattagact
    38521 tccacaatca gggcaagatg gagcctcata atccagctta gcgataattt c
//
LOCUS       DAVKWS010000014        38294 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKWS010000014 DAVKWS010000000
VERSION     DAVKWS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.014"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            21..866
                     /locus_tag="VW852_000764"
     CDS             21..866
                     /locus_tag="VW852_000764"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV0271939.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VW852_000765"
     CDS             complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VW852_000765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV0271940.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2422..4746
                     /gene="pflB"
                     /locus_tag="VW852_000766"
     CDS             2422..4746
                     /gene="pflB"
                     /locus_tag="VW852_000766"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV0271941.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5113..5331)
                     /locus_tag="VW852_000767"
     CDS             complement(5113..5331)
                     /locus_tag="VW852_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271942.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(5427..5665)
                     /locus_tag="VW852_000768"
                     /pseudo
     CDS             complement(5427..5665)
                     /locus_tag="VW852_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5658..5942)
                     /locus_tag="VW852_000769"
     CDS             complement(5658..5942)
                     /locus_tag="VW852_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271943.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(6554..6724)
                     /locus_tag="VW852_000770"
     CDS             complement(6554..6724)
                     /locus_tag="VW852_000770"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0271944.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            complement(7539..8762)
                     /locus_tag="VW852_000771"
     CDS             complement(7539..8762)
                     /locus_tag="VW852_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0271945.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            8930..10252
                     /locus_tag="VW852_000772"
     CDS             8930..10252
                     /locus_tag="VW852_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0271946.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            10254..12140
                     /locus_tag="VW852_000773"
     CDS             10254..12140
                     /locus_tag="VW852_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV0271947.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            12308..12652
                     /locus_tag="VW852_000774"
     CDS             12308..12652
                     /locus_tag="VW852_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0271948.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            12662..14074
                     /gene="lacG"
                     /locus_tag="VW852_000775"
     CDS             12662..14074
                     /gene="lacG"
                     /locus_tag="VW852_000775"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0271949.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            14143..15822
                     /locus_tag="VW852_000776"
     CDS             14143..15822
                     /locus_tag="VW852_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0271950.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(15948..17387)
                     /locus_tag="VW852_000777"
     CDS             complement(15948..17387)
                     /locus_tag="VW852_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0271951.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(17391..18740)
                     /gene="trkA"
                     /locus_tag="VW852_000778"
     CDS             complement(17391..18740)
                     /gene="trkA"
                     /locus_tag="VW852_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV0271952.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIEREHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            18929..19777
                     /locus_tag="VW852_000779"
     CDS             18929..19777
                     /locus_tag="VW852_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV0271953.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            19792..20340
                     /locus_tag="VW852_000780"
     CDS             19792..20340
                     /locus_tag="VW852_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV0271954.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            20347..20559
                     /locus_tag="VW852_000781"
     CDS             20347..20559
                     /locus_tag="VW852_000781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271955.1"
                     /translation="MKIKEKTRKQVAGCSKHGFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            20661..22343
                     /locus_tag="VW852_000782"
     CDS             20661..22343
                     /locus_tag="VW852_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0271956.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVNEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            22328..23158
                     /locus_tag="VW852_000783"
     CDS             22328..23158
                     /locus_tag="VW852_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0271957.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            23351..23854
                     /locus_tag="VW852_000784"
     CDS             23351..23854
                     /locus_tag="VW852_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV0271958.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPDKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      23908..24142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            24307..24870
                     /locus_tag="VW852_000785"
     CDS             24307..24870
                     /locus_tag="VW852_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0271959.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            24884..25510
                     /locus_tag="VW852_000786"
     CDS             24884..25510
                     /locus_tag="VW852_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0271960.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            25538..25927
                     /locus_tag="VW852_000787"
     CDS             25538..25927
                     /locus_tag="VW852_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV0271961.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            25932..26381
                     /locus_tag="VW852_000788"
     CDS             25932..26381
                     /locus_tag="VW852_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV0271962.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            26509..27096
                     /locus_tag="VW852_000789"
     CDS             26509..27096
                     /locus_tag="VW852_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV0271963.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            27486..29093
                     /locus_tag="VW852_000790"
     CDS             27486..29093
                     /locus_tag="VW852_000790"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV0271964.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(29527..31158)
                     /locus_tag="VW852_000791"
     CDS             complement(29527..31158)
                     /locus_tag="VW852_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV0271965.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            31451..36430
                     /locus_tag="VW852_000792"
     CDS             31451..36430
                     /locus_tag="VW852_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV0271966.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKATSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKSRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKSAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            36520..37716
                     /locus_tag="VW852_000793"
     CDS             36520..37716
                     /locus_tag="VW852_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV0271967.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(37994..>38294)
                     /locus_tag="VW852_000794"
     CDS             complement(37994..>38294)
                     /locus_tag="VW852_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0271968.1"
                     /translation="LIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLHV
                     VGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT        11604 a         6802 c         8181 g        11707 t
ORIGIN      
        1 tataatatag ggagtaatct atgtatctta ttgaaatttt aaaatctatc ttcttcggga
       61 ttgttgaagg aattacggaa tggttgccga tttccagtac aggtcacttg attttagcag
      121 aggaattcat ccaataccaa aatcaaaatg aagcctttat gtccatgttt aatgtcgtga
      181 ttcagcttgg tgctatttta gcagttatgg tgatttattt taacaagctc aatcctttta
      241 aaccgactaa ggacaaacag gaagttcgta agacttggag actatggttg aaggtcttga
      301 ttgctacttt acctttactt ggtgtcttta aatttgatga ttggtttgat acccacttcc
      361 ataacatggt ttcagttgct ctcatgttga ttatctacgg ggttgccttc atctatttgg
      421 aaaagcgcaa taaagcgcgt gctatcgagc caagtgtaac agagttggac aagcttcctt
      481 atacgaccgc tttctatatc ggactcttcc aagttcttgc tcttttacca gggactagcc
      541 gttcaggtgc aacgattgtc ggtggtttgt taaatggaac cagtcgttca gttgtgacag
      601 aatttacctt ctatcttggg attcctgtta tgtttggagc tagtgcctta aagattttca
      661 aatttgtgaa agccggagaa ctcttgagct ttgggcaatt gtttttgctc ttggtcgcga
      721 tgggagtagc ttttgcggtc agcatggtgg ctattcgctt cttgaccagc tatgtgaaaa
      781 aacacgactt cacccttttt ggtaaatacc gtatcgtgct tggtagtgtt ttgctacttt
      841 acagttttgt ccgtttattt gtataagaaa aaccttgaag gggcaactct tcaaggtttt
      901 atactcttcg aaaatctctt caaaccgcgt cagctttatc tgcaacctca aaacagtgtt
      961 ttgagcagcc tgcggctagc ctcctagttt gctctttgat tttcattgag ctttaaaatc
     1021 cagtcatggt aatccccaat aggcggacac ctctttcttt cttgcttaat tcttcataga
     1081 gttgcagggc tatttggctt atctgactag catcttgtgt tttttgagca agactttttc
     1141 gtttggtaag agttgaaaag tcctcgtagc ggattttcaa aatgacaatt tttccagctt
     1201 tttcttgttg atgtagattg agagcgactt tttctgatag aagagtcagc tcttttttga
     1261 tatcttcctc agcaaggaga atcttcccgt aggttttctc cttgccgatt gatttacgga
     1321 tgcgattgga tttgactgga gagttgtgaa tgccacgagc ctttcgatac agatcatagc
     1381 ctagtctacc aaaacggtct attagggtta cctcaggaac ttcaagtaaa tcagcaccag
     1441 taaaaacgcc catttgatga agacgttcta ctgtcttttt tcctactcca tgaaatttgg
     1501 aaatatccat ttgtttgaga aaatcctcag cctgttcagg tagaatcact gtcaaaccat
     1561 gtggtttttg ataatcactc gccattttag ctaagaattt gttgtaagaa acgcctgcgg
     1621 aagcagttag atggagttct tgccagatat ctttttgaat gaggcgagca attttgaccg
     1681 ctgacttgat accgagttta ttttctgtca catccaaata ggcttcgtca atgctcatgg
     1741 gttcaatcaa atctgtatag cgcttaaaaa tagctcgaat ctggagtccc acagacttgt
     1801 atttctcata attccctgag ataaagacag cctggggaca acgttcataa gcttccttgg
     1861 aactcatggc agaatggaca ccaaaagctc ttgcctcata actacaggta gaaacgactc
     1921 cccgtccacc tgtttgccga gggtcgcttc caataatgac aggttttcct ctgagtttag
     1981 gattatccct gatttccact gcagcaaaaa aggcatccat gtcaatatgg atgatttttc
     2041 ttgacaaatc atttaacaaa ggaaaaatca acatgcctag caccttttta tactcttcga
     2101 aaatctcttc aaaccacgtc agctctatct gcaacctcaa aacagtgttt tgagcaatct
     2161 gcggctagct tcctagtttg cttttcgatt tccattgagt gttactgctt attttctttt
     2221 attataccct tttttctgaa aaaaagaaaa aaggacttta ttttttcaaa aatataatac
     2281 agtttgaaat aaaatataga ctgttttaga aaagaaagtg taaaaatagg gaattttcac
     2341 ttgttgaaat cggttactat atggtatact tgtcttatga atgtaacaga tgactgttac
     2401 tagaaaaaag aggacattaa tatggttgtt aagacagttg ttgaagcaca agatattttt
     2461 gacaaagctt gggaaggctt caaaggcgta gattggaaag aaaaagcaag tgtatcacga
     2521 tttgtacaag ctaactacac accttatgat ggagacgaaa gcttccttgc aggaccaaca
     2581 gagcgttcac ttcacatcaa gaaaattgta gaagaaacta aagcacacta cgaagaaact
     2641 cgtttcccaa tggacactcg tccaacatct atcgctgata tccctgctgg atttatcgac
     2701 aaagaaaatg aagttatctt cggtatccaa aacgatgaac tcttcaaatt gaacttcatg
     2761 ccaaaaggtg gtatccgtat ggctgaaact actttgaaag aaaatggata cgaaccagac
     2821 ccagctgttc acgaaatctt cactaaatat gtaacaacag ttaacgacgg tattttccgt
     2881 gcctacactt caaatattcg tcgcgctcgt cacgcacaca ctgtaactgg tcttccagat
     2941 gcatactcac gcggacgtat catcggtgtt tacgcacgtc ttgctcttta cggtgcagac
     3001 tacttgatgc aagaaaaagt aaatgactgg aatgcaatca aagaaatcga tgaagaaaca
     3061 atccgtcttc gtgaagaagt aaaccttcaa taccaagcat tgcaacaagt tgttcgcctg
     3121 ggtgaccttt acggggttga tgttcgcaaa ccagcgatga acgtgaaaga agcaatccaa
     3181 tgggttaaca ttgctttcat ggctgtctgc cgtgtgatta acggtgctgc tacatctcta
     3241 ggtcgtgtac caatcgtatt ggacatcttt gcagaacgtg accttgctcg tggtacattt
     3301 actgaatcag aaatccaaga attcgttgat gatttcgtta tgaaacttcg tacagttaaa
     3361 tttgctcgta caaaagctta tgaccaattg tactcaggtg acccaacatt catcacaact
     3421 tctatggctg gtatgggtaa cgacggtcgt caccgtgtta ctaagatgga ctaccgtttc
     3481 ttgaacactc ttgacaacat cggtaactct ccagagccaa acttgacagt tctttggact
     3541 gacaaattgc catacaactt ccgtcgctac tgtatgcaca tgagccacaa acactcttct
     3601 atccaatacg aaggtgtaac aacaatggct aaagacggat atggtgaaat gagctgtatc
     3661 tcatgctgtg tgtctccact tgatccagaa aatgaagaac aacgccacaa catccagtac
     3721 ttcggtgctc gtgtaaacgt tcttaaagcc cttcttactg gtttgaatgg tggttacgac
     3781 gatgttcaca aagactacaa agtatttgat atcgaaccaa tccgtgacga agttcttgaa
     3841 tttgaatcag ttaaagcgaa ctttgaaaaa tctcttgact ggttgactga cacttacgta
     3901 gatgccttga acatcatcca ctacatgact gataggtaca actacgaagc tgttcaaatg
     3961 gccttcttgc caactaaaca acgtgccaac atgggattcg gtatctgtgg atttgctaac
     4021 actgttgata cattgtcagc tatcaaatac gctacagtta aaccaatccg tgacgaagat
     4081 ggctacatct acgattacga aacaatcggt gactacccac gctggggtga agatgaccca
     4141 cgttcaaacg aattggcaga atggttgatc gaagcttaca caactcgtct acgtagccac
     4201 aaactataca aagacgcaga agctacagta tcacttttga caatcacatc taacgttgct
     4261 tactctaaac aaactggtaa ctcaccagtt cacaaaggtg tatacctcaa cgaagatggt
     4321 tctgtgaact tgtctaaact tgaattcttc tcaccaggtg ctaacccatc taacaaagct
     4381 aaaggtggtt ggttgcaaaa cttgaactca ctttctagcc ttgactttag ttatgcagct
     4441 gacggtatct cattgactac acaagtatca cctcgcgctc ttggtaagac tcgtgatgaa
     4501 caagttgata acttggtaac aatccttgat ggttacttcg aaaacggtgg acaacacgtt
     4561 aacttgaacg ttatggactt gaacgatgtt tacgaaaaga tcatgtcagg cgaagacgtt
     4621 atcgtacgta tctctggata ctgtgtaaac actaaatacc tcactccaga acaaaaaact
     4681 gaattgacac aacgtgtctt ccacgaagtt ctttcaatgg atgacgcctt ggatgcattg
     4741 agctaatcaa gttcttgaat aataaaaagg aaccctcggt caatcgactg agggttttgt
     4801 gcttgggata gtgtgagcaa ttcctttgac gcaatatgca atgtttttgg gaaggaatag
     4861 tggaccgaat ctgaaatata gtagattgaa actagaatag tacaccttta tttctaaaac
     4921 attcttagaa atcgatttga ctgtcctgat cgatttgtcc tattcttatt tcattttact
     4981 atagtccact gaaacgtctc aaacagcgct agaaattgac tggaattctc taagcgattt
     5041 gttcagattt tatttcattc catcataaaa ataagacgac tggctaattg acaatcatct
     5101 tgtttgatac tgttaataaa attcgcgaag tttatcttga ttttctttaa aaaatttttc
     5161 taaccaatcc ataccataag cataactaaa accttctaat agaacagaga ggtcaatagt
     5221 atcaaatccc atgtctttta aatatttaaa ggtatcgaat ggtgaatgaa agtttttgta
     5281 tttaataatt gcaccaatat ctttcaaatc ctgctctctg atactcatca tcttcattcc
     5341 taacacatac tcaataggga ctactaaaac ggtcagattc tcaaatgagt acaaggattc
     5401 gcataaactt aagggaggtt gtttgttcat atttgctttg caacatgatt gttgagccat
     5461 aactcttcgt ggatgttaag attaaactgt tttcctacac gagcaataat ttcattgatt
     5521 ttctgatttt ggtcataaaa agcatcaaca tcttgtgtgg cacgtaaacc atgatattct
     5581 aagacataac cataattgtt aaagtcagat tttcctgaat taatttctta tttagggctt
     5641 ggaagaccgg tttcatttta tccatgtttg tatttctcca tcaatttttg aacattggtt
     5701 aacttagcag gtttatttaa tgaaatcaca tatttatgag ttggagtatg ctcttgaaag
     5761 gcatacttca agctaatttt ttgaaaacgg gttagttctt tggagtctag caacttttct
     5821 acatcttggt ttgttaaata agaatagtct tcaaaaataa cttttcgcaa atagcgaaac
     5881 caatggcttg cgttgaattg tgattgtttg gatgcctgta tcatataatc gtaccaatcc
     5941 attgtcttcc tcctataaaa tttagtttat attattgtag tttatattca aaaatgtagt
     6001 ttttcttaat tagctcgtat ctataggatg atactgaaaa tcttcttgtt gctttaaatc
     6061 acaatcgaaa aattctgttt ttcatcttag aaaagaatgt ttagaaatgt aaaatgaatc
     6121 ttttttctta tccgaaaaca ttactaaagt atataattta ggaagtcaat tctgtatgat
     6181 aatgtttgta ctatcgtcga gtttttgctt gattgcctaa cgagataaat tttagaagaa
     6241 ttttcggtgt gatttgacaa gcttctgact ggaaggatag ttacactagt attgtttatt
     6301 aaaatgagca gatagagaaa atttggaatc tatttgctta ttatcaatcg tttttttagc
     6361 ttatttgtct tagattggta tagaactgag ctatggatta atactcttgt atcttatcct
     6421 gttcctaaat atgttggtag aaaaatgaag cagttgttac gagtgtctgt attggaaaaa
     6481 attttatcta tacaattctt taaatgatat gaaggctagg aatgccaaaa cactgacata
     6541 tttcgtttaa agcctatcca gtctcttcaa ttgtatttgt agaaatgcta tcagtaatat
     6601 attcgcgaac actatgattt acaacagtgt ggtcgtacat ctttgtaact ttattataca
     6661 acaaccattt acaggtgtaa agttgagcag tgtctttagt aaatttaggg acttgttttg
     6721 tcatagtttt gatacctgta ttcgttgttt catgagcatt tgacgtttct tttcatgtag
     6781 gcatttcaac aattagaatg atatttttcc gaggatgaac ttatatattg tgacttttta
     6841 gagttatgat gcctcttgat ttgactatcg ctatcaattt catccacttt tacagtttca
     6901 tggttttatg aaccgaaaca tctaggtaca ggttttagca tcaattgatt gtttttgagc
     6961 catatgaagt tgaaacgtgg agatgctttt cttgtgataa cgccctattc aaagaggcat
     7021 aaccgttttt ggagagagtt cagtactatg tggcgctaaa taatatatgt aaaatagtat
     7081 ggtgtagaga tattaaacaa gatttaacta atacttttcg aaaatctctt caaaccacgt
     7141 cagcgtcgcc ttaccgtata gatgttactg acttcgtcag ttctatctac aacctcaaaa
     7201 cagtgttttg agcagcctgc agctagcttc ctagtttgct ctttgatttt cattgagtat
     7261 aaggagaatt gtaaaaacaa aaatggggat ttcatataaa gtttattcta ggagtatgta
     7321 tcttgaataa aaattgttat ttaagttctg ggggttatga tgaatgtttt tataataata
     7381 aaatgcagct agaagaaaag ttattttaat ataaatcact caacatagcg agaaggcggt
     7441 ttcctatatg gaagtcgtct tattttcata ttgtctaaat acgagatatt tactacaggt
     7501 tatagttgat ttaaacaggt ctatagattt ctaatatatt aaggtgaaat aatatcttgt
     7561 aatacattgc tatgttctat gaaaaaagcg acgatagcta atgcacaagc tcctatactt
     7621 ccacgatgtt tgttgtaagg taaaatttca atttctatat tacgagtaaa aggaacgaag
     7681 tggagctgat cttggatttt atccctgact tcatgagtga aaggttgata attaagcaat
     7741 tgactgttga tatagatttt atcagcagga ttgatgagga gaagatttgc aatagaagtg
     7801 gccaataaat tgactccttt atcaatttgt tggcgtaaag cggagtcgcc taagttgtaa
     7861 gctgttaaaa gggtatctaa atgaatgtct ttttcagtct ttacaaggct ttttagtaca
     7921 gttagttggg aatttttaaa taataattgg gcgtgtttga ttaaccaagc atccgaaata
     7981 tatgtttgta aacaaccttt ttttccgcat tcacaatatt gcccattcaa ttcgacaatg
     8041 gtgtgtccaa tttctccgat ataaggattt ttagaggctc ctattttttc ttttgtgaaa
     8101 aaggaagtgt aaatacctaa tccagcgtgt aggaaaataa agttatcggg ggtattgtgt
     8161 ggattaaaaa ggtattgtcc tatagccata caatcgatat tattttttac aataaaagga
     8221 aaattgaatc ttttaattac atttaataaa ttaaaagatt cccatatggg gttgtttgta
     8281 atgatacttc cggagtcttt gttataatgt ccaggtattg ataagccaaa tccaagtaat
     8341 tttgtttcca aagggacgta ctctatagct gtttttatca tattggtaat ggtggaattg
     8401 atttctgaaa taggataatc ttcattcaaa atttctgtct cgaaaagagt tttcccttgt
     8461 aagtcacata gactgagaaa gatacctagg acgtttattt caatacctcc aacaacatac
     8521 ggaatattat ctgtgataga aatagttttt ctacgtcttc cagatccgga aaactcgcta
     8581 aattcatcac cagtttcgta tatgagggac tgttcgctta gctcttttat gatgttagtc
     8641 gttgttgctg gagtaagtcc tgtttcatta acaatatctg tacgggatgt tgctggattt
     8701 cgatatagaa actccagtat agtggctcta ttttgtaaaa aggctgattg ttttgtaatc
     8761 atttgtttcc tcctttggtt tattttatat taaacataag aaaaaatcaa ttaaaattat
     8821 tattctatac aaagaaaaaa atattgtaaa aacaacaaaa aatatattga caacgctttc
     8881 tgttagtggt atatttaatt tataaaaaat aaaaaaagga gttccggtga tgaacaatat
     8941 tttagcgttt ttagaaacaa aagtcgctcc gtttggtgaa aaagttggca accaacggca
     9001 tttgaaagct attcgtgaag gatttatgat ggcaatgcct ttgattttag tcggctcttt
     9061 atttcttatt ctaatcagtt ggcctcaaga ggcttttaca aattggctga atagtgttgg
     9121 attgctaagt atcttgacaa ctatgaatca gtcaacagta gcgattatct ccttggtcgc
     9181 ttgtttcggt attgcctaca ggttgtcgga aggatatggt acagatggtc cgtcggcagg
     9241 gatcatagcc ttatccagtt ttgtattgat ggcacctcgt ttttcgagta tggtttatga
     9301 taaaaatggg gagcaggtca agcagttatt tggcggcgca ataccatttt ctagcctgaa
     9361 tgcatcttct ttgtttatgg cgattactat tggattggtt acagcagaga tttatcgtat
     9421 gtttatccag cgcggaatta cgataaaaat gccaagtggt gtcccagatg tagtaagtaa
     9481 atcattttca gctcttttac ctggttttac tacttttgtt ttgtgggctt tggtcttaaa
     9541 aggtcttgaa gcggcaggag ttgcaggagg tctcaacgga ctcctaggtg caattgttgg
     9601 aacaccgctt aagttaattg caggaacgct tccaggtatg attctatgtg ttattgtaaa
     9661 ctcattcttt tggttctgtg gagttaatgg gggacaagtt ttaaatgctt ttgtagaccc
     9721 agtttggtta caatttacta cagaaaacca agaagctgtg gctgcaggac aaacactcca
     9781 acacattatt acattaccgt ttaaagattt atttgtattt attggtggcg gtggagcgac
     9841 tattggtctt gcgatttgtc tcttcctatt tagtaagagt cgtgcgaata aaacattagg
     9901 taagctagct attataccgt ctatttttaa tatcaataca gctattctat ttacgtttcc
     9961 aacagtttta aatccgatta tgctgattcc gtttattgct actcctacaa tcaatgcctt
    10021 gattacctat gtatcaatgg ctgtaggatt agtaccctat acaacaggtg taatccttcc
    10081 gtggacaatg ccaccgatta taggaggctt ccttgcaaca ggggctagtt ggcgaggagc
    10141 tctattacaa gttgttttga ttttggtttc tgtagcaatt tattatccat tcttcaaaat
    10201 tgcagataaa cgcaatcttg aaaaagaaaa agctactgtt ggagggaaat aagatggtta
    10261 tcagagtatt tgatcaacag aaaaatactt attctagctt tgccttagag gaattaagtt
    10321 actatatgaa tcgggtcttt aagactaaca tagagcttgt cgaggagaag gaagcggata
    10381 tttttgtagg attagtcaat aaagaggaca gaaaagacca tgttcttatc tcattagaca
    10441 agggtaaggg gagaattgag tctaatacaa ttgtaggttt acttattgga atttaccgaa
    10501 tgtttcatga atttggggtt gtgtatacta gaccagggcg cagacatgac tttgttccag
    10561 agttacgatt tgaagatttt ttagataaac agctatctat agatgaaaca gccagttact
    10621 atcatagggg agtatgtata gagggagcgg attcatttga aaatatacta gatttcattg
    10681 attggctacc taagattggg atgaacagtt ttttcatcca gtttgaaaat ccttactctt
    10741 ttttgaaacg ttggtatgaa catgaattta atccatatct aaataaagaa caattttcaa
    10801 atgaattagt acaagaattg agtgataggt tggataaaga attgcaaaaa agaggtctta
    10861 ttcatcatcg tgttggtcat ggatggacag gtgaagtttt aggttactct tcaaaatttg
    10921 gctgggaatc aggtcttagt atttcagagg agaagaaacc ctatgtcgct gaaataaacg
    10981 ggaaacgaga attgtttaat acggctccga ttttaaccag cctggatttt tcaaatccag
    11041 atgtagctga taagatggta gaaattatca aggattatgc caagaaaaga cctgatgtta
    11101 actacttaca tgtatggttg tcggatgctc gtaataatat ttgtgaatgc gaaaactgta
    11161 gacaagaatt ggtttcggat cagtatattc gtattctcaa tcaattggat agggctttaa
    11221 cgagtgaggg attagataca aagatttgtt ttctgcttta tcatgagttg ttatgggcac
    11281 ctcagaaaga aaaattagat aatcctgaac gctttaccat gatgtttgca ccgattacaa
    11341 gaacatttga aatgagttat gcagatgtag attttgacaa ttccatacct acgcctaaac
    11401 cttatatgcg taataaaatt atacttccga attctcttga ggaaaattta tcttatcttt
    11461 ttgagtggca aaaagcattt aaaggagata gtttcgtata tgactatcct ttagggcgtg
    11521 ctcattatgg cgatttaggc tatatgaaaa ttagtcaaac tatttacaga gatgtatctt
    11581 atctttccaa cctacatttg aacgggtaca tttcgtgtca agaattacgt gccggattcc
    11641 ctcataattt tcctaattat gtcatggggg aaatgctctg gaagaagaca agaagttatg
    11701 aagaattgat tgaagaatac ttttctgctt tgtatgggga aaattggcag tctgttgttg
    11761 aatatttaga aaaattatcc atttattcct cttgtgatta ttttaatgca attggcagcc
    11821 gtcaaagtga tgttttagcg aatcattatt atatagctta caatctagct gataattttt
    11881 taccaattat tgaggaaaat atttctaagt tattaaatag tcaaaaggat gaatggaaac
    11941 agctcagtta tcatcgtgaa tatgttgtta agatggcgaa ggctttatat cttcaagcaa
    12001 ctggaaaaac aaggcaagct caagatgaat ggagaaatgt gttgaattat atccgtgggc
    12061 acgaattgct atttcaatct aatttggatg tttatcgtgt aattgaagta gcaaaaaatt
    12121 acgctggttt ccacttataa atcataagta tagaaaatga actaaggtat tcagagaaga
    12181 ttgatcctaa atattatgaa atttaaggat ttttaagata tttagggtcg actttctatt
    12241 tatatcgtag cgaagtcatt ttaataatga tgtgtaaaag atggatcaag attgaggagg
    12301 aagaaagatg aaatcaaaag aagaaataaa tatgcttggt tttacaattg tcgcttacgc
    12361 aggagatgca aggtcagatt tgatggatgc tttggcgttt gcgagagatg gatattttga
    12421 acaggcaaga gaattggttg agtctgcaaa cgactcaata gtgtctgccc atcgagaaca
    12481 gactaattta ttagcggagg aggcatctgg agataatttt gaagtgagtt ttatcatgat
    12541 tcatggtcaa gataccttga tgacaacgat gctattgtat gatcaggtca agttttttat
    12601 tgatgaatat gaacgaattc gaaagattga agaacatatt ggtttgcaat gaggattagt
    12661 catggaaaat ttacaggtta aagccttacc gaaggagttt ttattaggag ctgctaccgc
    12721 tgcttatcaa gtagagggtg caactagggt agatggcaaa ggaacaaata tgtgggatgt
    12781 ttatttgcaa gaaaacagtc cgttcttacc agatccagct agtgattttt attatcgtta
    12841 cgaagaggat ataactttgg cggcagaaca tggtttgcag gctttgcgtt tatctatttc
    12901 ttgggttcgt atatttcctg atatagatgg ggagcctaat atattagctg ttcgttatta
    12961 ccatagagtc tttcagtctt gcttaaaaca taatgtgatt ccgtttgttt ctttacatca
    13021 ttttgattcg cctcagaaaa tgttagaaac aggggattgg ttgaacagag agaatattga
    13081 tcgtttcata cgatatgctc gcttttgttt ccaagaattt acagaagtca agcattggtt
    13141 tacaatcaat gaactgatgt ctcttgctgc aggtcaatat ataggaggtc agtttcctcc
    13201 aaatcatcat tttcaattat ctgaagcaat tcaagcgaat cataatatgt tgttggcgca
    13261 tgctcttgca gtcctcgaat ttcatcaatt agggattgag ggaaaggtag gttgtattca
    13321 tgctttaaag ccaggctatc ctattgatgg gcaaaaagaa aatattttgg cagctaaacg
    13381 gtatgatgtt tataataata aatttctatt agatggaact tttttgggct actacagtga
    13441 ggacacgctt tttcacttga atcaaatatt ggaagctaat aattctagct ttattattga
    13501 agatggtgat ttagaaatta tgaagagagc tgcacctctt aatacgatgt ttgggatgaa
    13561 ttattatcgt tcagaattta ttcgtgaata caaaggtgaa aatagacaag aatttaattc
    13621 aacaggaata aaaggacagt cttcttttaa attaaatgct ctaggtgaat ttgtaaaaaa
    13681 acctggtatt ccgacaacag attgggattg gaatatttat cctcaagggt tatttgatat
    13741 gttgcttcgt atcaaagaag aatatcctca acatccggtc atttatttaa ctgaaaatgg
    13801 tacagccctt aaagaagtta agccagaggg cgagaatgat attattgatg acagtaagag
    13861 aatccgttat attgagcaac atttacacaa agttttagag gctcgagata gaggagtcaa
    13921 tattcaaggc tattttatat ggtctttgca agatcaattt tcttgggcga atggctacaa
    13981 taagcgatat ggtcttttct ttgttgatta tgaaacacag aagagatata ttaagaaaag
    14041 tgctctttgg gtaaaagggc taaaacggaa ttaaggttag cgatttgact gatgtttaat
    14101 atgttttaaa tatgaggttg aattttttat aggaggagtt ttatggataa gctagtcgct
    14161 gccattgaaa agcaacaagg gaaatttgaa aaaatttcta ctaataacta tatgatggct
    14221 attaaagatg gattcattgc tactatgcct ttaattatgt tttcaagctt tttgatgatt
    14281 attattatga ttcctaaaaa tttcggagta gagttaccga gtccagctat tgcctggatg
    14341 aggaaagtgt atatgctaac catgggagtt ttgggtatta ttgtttcagg gacagttgca
    14401 aagtcattgg ctggaaatgt taatagaaaa atgcctcctg gaaaggtaat aaatgatact
    14461 tctgcaatgt tggcggccat atgtggctat cttgtattaa ctgtaacgct tgtagttgat
    14521 gagaaaacgg gatctacaag tttgtcgaca aactatttag gatctcaagg attgataact
    14581 tcgtttgtca gtgcctttat tactgtaaat gtttaccgat tctgtattaa gcgagacatt
    14641 actattcatt tacctaagga agttcctggg gctatatcac aagcttttag agatattttt
    14701 cctttttctt ttgttttact tattagtggt ttgttagata ttgtatctcg gtttagttta
    14761 gatgttcctt ttgcccaagt atttcaacaa ctattgactc ctatttttaa gggggcagaa
    14821 tcatatcctg ctatgatgtt gatttggttt atgtgtgctt tgctttggtt tgttggaatt
    14881 catggaccat ctattgtctt acctgctgtt acagctttac aactgagcaa tatggaagag
    14941 aatgctcaac ttcttgcaaa tgggcagttc ccttatcatt ctttaacacc taatttcggg
    15001 aattatatcg ctgctattgg aggaacgggg gctacctttg ttgtaccatt tattttgatt
    15061 ttctttatgc ggtctaaaca attaaaatcg gtaggtaaag ctacaattac tcctgtttta
    15121 tttgcggtaa atgaacctct tctatttggt atgcctgtta ttttgaatcc ctatcttttt
    15181 gtcccttttt tgatgactcc accagtgaat gtatttctag gaaaggtatt tattgatttc
    15241 tttggaatga atggatttta tatccagtta ccttgggcct ttcctggtcc cttgggattg
    15301 ttaattggaa cgaattttca acttatctcc tttgtatttt tatctttgat gttagttgtc
    15361 gacatattga tttatttgcc attctgtaga gcgtatgata gacagttact ggtgaaagaa
    15421 gatattgcaa gctcaaatga tattatttta gaggaggata caagtgaaat aatgcctggt
    15481 gagatagatg taataaaaag taaggagttg aaagtactgg ttctttgtgc agggtctgga
    15541 acaagtgcgc aattagccaa tgcaattaac gagggcgctc aattagcaga agttagagtg
    15601 attgcgaatt caggagcgta cggagctcat tatgatatta tgggtgttta tgatttaatt
    15661 attctggccc cacaagttcg gagttattat agagagatga aggtggatgc agaaagatta
    15721 ggtattcaga tagttgctac cagaggaatg gaatatattc atttaacaaa gagtccaagt
    15781 aaagccttac aatttgtatt ggagcattac caagctgtgt agtaagtttt tccatctttt
    15841 atttgagtaa agattttgct tacagatagg cttggattta aaaacgttcc ccctttttta
    15901 atataagaat ccctctttca caattgtaaa aagagggatt ttgtatttta tctcttagac
    15961 caagttctct tcataaagag aaggaggatt gggtaaatct ccaagcgccc tgcaatcatt
    16021 gcaaaggata ggagaatttt tgagatggga ctaaagattg agaaactaga agtggttcct
    16081 agaataggcc cgatattatt gaaacagcta aagacagcgc tggtcacgac cagaaaatca
    16141 ttgctatcta ggctgacaat aaagataagc gctagcaaaa tcatagcata gatgacaaag
    16201 tacttgagaa tcttatgctg ggtatctttg tcaatcaccg ttttattaac atggagggtc
    16261 aaaacacggt ggggcgatag gattgacaaa atttggtttt tggcaatttt tgaaaggatg
    16321 aggcctcgaa taatcttgag tccacctgca gttgatccag cagagccacc gattgccatg
    16381 aggaaaagga ggataaactg ggagaagagg ggccagttgg taatatctcc atatccaaaa
    16441 ccagttgttg taatgatgtt ggaaacctgg aagaaggtca tttcaaagct ctttgaaaac
    16501 cctgggtaga ggtagagggt gttgaggcta atcaagcctg tagaaaccag tacaatgacc
    16561 aagtaagccc taagctcttc atctccaaag aaggccttga tgcgacggag catgaggtag
    16621 tagtagaggt tgaaatttac tccaaaaacc agaactccga tactgaccag ataggtaatc
    16681 agtgagctgc catagtgggc aattccgtcg ttatagacgg taaagcctcc agttcccgct
    16741 gtccccatag caataacaaa actatcgtag agaggcatac cggctagata atagatgatg
    16801 acaaagaggg agaagagagc tagataaagg agatagagaa tctgggcagt gttttttagt
    16861 ttggatacaa ccttgccaaa aacaggacct ggaacctcag ccttcatcac ctctaggtgg
    16921 ctatttttgg cattgtccat aatagcaagt gcaaaaacaa gcactcccat ccctccaatc
    16981 aagtgggtaa aacttcgcca gaagaggagg gaacggctga gaaccgaaac gtcgttcaaa
    17041 atacttgctc cagtagttgt aaatccagaa ctaatttcaa aaaaggcatc aataaggctg
    17101 gggatttgcc cagaaaagac aaaggggaga ccaccaaaga aagaccaaag gatccaacag
    17161 agggcaacga tcaagactcc ctccttggca taaatccgtt gattttttgg cttctgtaaa
    17221 ctccctgaac cgcctaacaa tacgagaatc cctatggtcg aaaagagggc tgtaaagact
    17281 tggctcgatt cacggtaata gacagcaatc gcaacaggaa ccaaaagaag aacagcttca
    17341 atcaaaagta attttgaaag gaggtaacga atcatacttt tattcatttc ttacctcgcg
    17401 atcaagtcat aaatcttggt gatgtttggc aacaaggttg ttactaggag cttgtctcca
    17461 acttccaaca tatcctcccc agttgggaaa atagtcttgc cctttcgaat aatggctgca
    17521 ataagaaccc ctttttttaa tttcagttga gaaagaggtt tggcagtcat tttattggct
    17581 tccttgatat ggaattgcag ggtttcgatt tggccattgg ctagatggtg catggcttga
    17641 aggtctgaat actgggcatt aactcgacca cgaataaagt gcataatcgt atctacagcg
    17701 atgcttttag gtgtgatgat acttgaaaaa tcaggcgcat tgataatctc gaggagactg
    17761 gtacgattga ccttagtaat atttttctgt acacctaccc tgtcaaggaa catagatgta
    17821 atcagatttt cctcatcgac acctgttaga gtcgcaacgg catcatagtg ttgagcactt
    17881 tcttccagca ggatatcttt tgcggttcca tctccttgaa cgatgtagag atttgggaat
    17941 ttctcgctaa agaagctggc gatttcagga ttgatttcaa tgacttttgt atcgatacga
    18001 ctatctttga gaataccaag tagataatag gcaattctac ctgccccaac gatgagaagg
    18061 ctcttcacgg cgcgtgattt aaaataatta tggaagagta tcatatcgac acggttacca
    18121 gtgacaaaga ttctatcttt atcctgtaca gtcatgtcac cgcttggaat gataatttga
    18181 tgctccctct ctatcgcaca gacaatgaca ttaccaaatt ttttacgaaa atcagaaatg
    18241 ggcatttggc aaagaccgct ggtggacttg acgacaaatt ccatgaggct aacgcgtcca
    18301 ccagcaaagc gttcgacaga cagggcgttg gggaagtcaa tgatattcgc gatagcgcgg
    18361 gcagccaaga gctcaggatt aacgataaga gaaaaaccga gaatattctt ttccttgaaa
    18421 taagagttag aatattcagg gttccgcacc cgaacgatag tttctttagc tcccattttc
    18481 ttggctagaa ctgctgcaat catgttgact tcatcgtgct cagtcagggc gataaagata
    18541 tcacaatctt ggacgctggc ttgctcaaga atggcaaaat cggccccgtt accaaggata
    18601 ccaatgatat caaagcgact gacaatatga ttgagaacag cttcgtcttg ctcaatcagc
    18661 aaaacatcat gcttttctgc aaccaaggag cgacagaggg caaaaccaac ttttccccct
    18721 ccgacaagga taattttcat aataaaacct actttttcat gatgtaacta tcataccctt
    18781 tttcaagaaa aaatgcacct actagctaat aaccagagtt tttagtagga aatttgctat
    18841 aagttaaaac tataccctaa ccaattgaaa tagctattag cgactttctc tgaaatatgg
    18901 tatgataaag gatatacaag gagataaaat gaataataat ttactggtat tacaatcaga
    18961 ctttggtctg gttgatggtg cggtatcggc tatgattgga gtggctttag aagagtctcc
    19021 aaccttaaaa atccatcact tgacgcacga tatcacgcct tataatattt ttgaggggag
    19081 ctatcgtctc tttcagacgg tggattactg gcctgaggga acgacgtttg tatcggttgt
    19141 cgatccaggt gtcggttcga aacgtaagag tgtagttgcc aagactgcaa aaaatcaata
    19201 cattgtcacg ccagataatg ggacgctttc ctttatcaag aaacacgttg gcattgtagc
    19261 cattcgtgag atttctgagg tggccaatag gcgtcaaaac acagagcatt cttatacctt
    19321 ccacggtcgt gatgtctatg cttatactgg tgctaaactg gccagtggtc acattacttt
    19381 tgaggaagta gggccagagc tcagtgtgga acagattgta gagcttccag tcgtagcgac
    19441 catcatagaa gatcatctgg tgaagggagc cattgatatt ctggatgtgc gtttcggttc
    19501 gctttggacc tctatcacac gggaagaatt ttacaagctg gaaccagaat ttggtgatcg
    19561 ttttgaagtg accatctatc atgctgatat gctggtctat caaaatcagg ttgtctatgg
    19621 caaatcattt gcagatgtga gaattgggca acccatcctt tacatcaact ctctctatcg
    19681 tttaggtttg gctatcaacc aaggttcctt tgccaaggcc tataatgtag gtgtcggttc
    19741 atcttggacc attgaaataa agaaaataga aggataaaat aggagaatag tatggaaatc
    19801 aaatttacaa ttaaacaagt tgttgctgtc ggaattggcg ctgccctctt tgtcgtcatc
    19861 gggatgatca acattccaac ccctgttcca aatacaagca tccagcttca gtatgcggta
    19921 caagcgctac tttctattat ttttggaccg attatcggtt tgcttgtcgg gttgattggt
    19981 catgcaatca aggactctct tgttggttat ggtctatggt ggacttggat tatcgctagc
    20041 ggactctttg gcttagttgt gggacttttt agaaagtacg ttcgcgtgat caatagtgtt
    20101 tttgactgga aagatattct tatttttaat ctcattcaac tacttgcaaa tgcccttgtt
    20161 tggggtgtct tggcaccact tggagatgtt gtgatttatc aagaagcggc agaaaaagta
    20221 tttgcacaag ggattgttgc gggaattgcc aatggtgtat ctgtagctat tgcaggaact
    20281 cttctcttac ttgcctatgc aggaacccaa actcgtgcag gaagtttgaa aaaggactaa
    20341 tactcaatga aaatcaaaga gaaaactagg aagcaagtcg caggttgctc aaaacatggt
    20401 tttgaggttg tagatgaaac tgacgaagtc agtaaccata cctacggcaa ggcgaagctg
    20461 acgtggtttg aagagatttt cgaagagtat aaaatgatgg ggaaggctgg gcaactagtc
    20521 tttttcgatg tttatagatt agttaggcaa gtaagctagg ataggaatct ttttggcgct
    20581 aagattttaa gaggagagac tgcaagaagg gcttgattta tgataaaata gaagtctaag
    20641 aagcgaatga agagagtaag atgaaagaag ctataattga gtggaaggat ttctctttcc
    20701 ggtatgaaac acaacaagaa ccgaccttgc aagggataga cttgaccatt tacaagggag
    20761 agaaagtctt aattgttgga ccatctgggt caggtaaatc taccttgggt cagtgtttga
    20821 atgggattat tcccaatatt tacaagggtc agacatatgg agaatttttg ataaagggtc
    20881 aagtagcctt tgatatgagc atctatgata agtctcatct ggttagcaca gttttacagg
    20941 atacagatgg gcagtttatt ggcttgtctg tggcagaaga tttggcgttt gctctggaaa
    21001 atgatgtgac agccctagat gagatgaaag gtcgtgttta taaatgggct gaaaagctgg
    21061 accttcttcc tttactggat cagcgtcctc aggatttgtc aggtggacaa aagcagcgag
    21121 tcagtctggc tggtgtcttg attgatgaaa gtccgattct cttgtttgat gagccactcg
    21181 ccaatctaga tcccaagtca ggtcaggata ttatcgaatt gattgaccag attcataagg
    21241 aagaggggac gacgactctg attatcgagc accgtttgga ggacgttctg catcgccctg
    21301 tggatcggat tgtcttgata aacgatggtc gtatcctttt taatgggagc cctgaccagt
    21361 tgcttgcgac tgatttattg actcaaaatg gaattcgaga acccctttat ctaacgactc
    21421 tccgtcaatt aggtgtggac ttagtcaagg aagaacaatt agcagatctg gataacttgt
    21481 ctatctcaaa agggcaaatc cagttacaga cggaactggt aaaagaaacc ccagcattgc
    21541 agtcactctt tatactagag gaagtatctt tttcttataa tgatagaccg attttaaaat
    21601 ccctacattt agatattaaa aagggtgaaa agattgctat tgtcggaaaa aatggagcag
    21661 ggaaatcaac tctagccaag gctataagta gctttattca gacggaagga cgctatcttt
    21721 gggaaaaaca ggatataaaa ggcgattctg ttgcagagcg ggcggaacga gtaggctatg
    21781 tgctacaaaa tcctaatcaa atgatttcaa ccaatatgat ttttgatgag gtggctctag
    21841 ggctccgttt gcgaggtgtg aatgagaagg aaattgaaac gagagtctat gaaaccttga
    21901 aaatctgtgg actttatgaa ttccgtaatt ggcctatttc tgccctgtca tttggtcaga
    21961 aaaaacgtgt caccattgct tcaattttgg tcttaggagc tgaaattatt ctcctagatg
    22021 aaccgactgc aggtcaagat cagaagaact atactgagat tatggaattt ctcgaagagt
    22081 tacatcaaaa agggcatacc attgtcatga ttacccatga tatgcaattg atgctggatt
    22141 attcagaccg ggtccttgtc atggtggatg gagaattgat tgccgatact gttccagcca
    22201 gtctgttgag cgatcctgag ctgttagtaa aagccaatct aaaagaaacc tccatcttta
    22261 acttggctaa gaaactagat gtggatccac tggatttaac ggcattttac aaagaaagga
    22321 gagagggatg caagctaaat taatcggtta ccagcataga gatactgtga ttcatcgctt
    22381 gtcaggagct gggaaacttc tctttttcat tctggtatca ttggcggcca tgattagcta
    22441 tgataccaga atgctcttgc tgattgccat cttttcagtc tttctcctct atttatcaga
    22501 aatccgcttt aaagatgtct cctttgtagc cgtttttgcg acggtatttg ccgttttaaa
    22561 cgttttgatg gtctatctct tttctcccga gtatggggtt ggactttatg gagagagaag
    22621 tgtgatttgg cagggaatcg gtgtctacac tctaaccagc caggagctct tttatctgct
    22681 aaatttggcc attaagtatc tttgcaccat tcctctggct attatctttt tgatgacaac
    22741 ccatcctagt cagtttgctt ccagtttaaa tcaaattggt gtgccctata agattgctta
    22801 ttctgtcagc ctgaccttgc gctatattcc agatttgcag gaagaattct ttactatcaa
    22861 gatgtctcag gaggcgcgtg ggatggaatt atccaagaaa gcttctctta tgcaacgaat
    22921 caaaggcaat ctgctcatta ttacgccctt gatttttagc tcgctagaac gcattgatac
    22981 cattgcgact gccatggagc ttcgccgctt tgggaaagag aaaaaacgca catggtatag
    23041 ttatcaggcc ttgaaaaaag gagactatat taccttgctc ttggcaacct tgtttttagt
    23101 agttagttta ctacttatct tgcagaatca gggacgattt tacaaccctt ggaaatagct
    23161 tgaaaaaatt gaaaaaatca agtcatttct attgacaatg attctgaaag tgttatacta
    23221 agaaagtagt ttcgctgatt tacttcaaac ctgttgtgag gtaagttaac gatgccttaa
    23281 ccacgctgtt tgctgagctt gactccgggc agtgtggcta tttttttgca atggtgaaag
    23341 gaagcaagtc atgacaaatc acattgtatt atttgaacct caaattccac aaaatacagg
    23401 caatatcgcg cgtacttgcg ctgcgaccaa ttctcccctc cacatcatca agcctatggg
    23461 ctttcctatt gatgaccgca agatgaagcg ggctggattg gattactggg ataagctaga
    23521 gatttatttt tacgagagtt tagaagattt catatctcag atgaagggaa aactctatct
    23581 gatttcgaaa ttcgcggaaa aaatctattc tgaggtggat ttatcgactg acgaggacca
    23641 ttattttctc tttggacgtg aagacaaggg cttacctgaa gattttatgc gagaacatcc
    23701 tgataaggct ctccgtattc ccatgaatga tgaacatgtc cgcagtctca atgtgtctaa
    23761 taccgtctgc atgattgtct atgaggctct tcgccagcag aactttgcag gtcttgagct
    23821 cgtccatacc tatgaagtgg ataaattgaa ataaaaaatg aaaatgaaca aaatgcttgc
    23881 gcttgcaagc gttttttgtt atgataaaag agtcttcagg gcagggtgtg attcccgacc
    23941 ggcggtgact ttaactagga aatgatcttt tccttttata ctttgttgac aagctttgcc
    24001 taaccagaag ttatgcctac agcttgtcgc ctagtctaaa agaaaaatct ctatttcttt
    24061 ctctttaaag aagtccgcga gcgcaagctg atgtggtgag attccacaac cgacagtata
    24121 gtctggatgg gagaagacga aagaatagct ttgtctgttt tgatagattt atagccggat
    24181 tgtaaccact tgctttgttt ttctttaata gagtaagagg gaacttttgg gatataaaaa
    24241 gtgagaatag atagaggaat cctttccaac ttcttctgat tttatagaaa attggaggaa
    24301 cctgttatga caaacacacg tcgactttcg accattgcaa ttctatcagc catctcattt
    24361 gtgctgatgt actttgattt tccgctttta ccagcggcat ctttcctcaa gatcgaattt
    24421 agtatcttgc cagtccttgt gggtctggtg gtcatggatt tgcctgctgc tctaggagtg
    24481 ctcttgcttc gctcactctt gaagttgctt cttaacagcc aaggagtgaa tacttacatt
    24541 ggtttgccga tgaatatcgt agctttggga gtttttgtca tcgtatttgc tttgatttgg
    24601 aaaaaggaac ggacaaccct tcgtttccta ctaggctctc tagctggaac tattggttta
    24661 accttggcca tgttggttct caactatgtt tatgctgttc ctttgtacgc taagtttgct
    24721 aactttgata ttggaaaaat tttgggactt tccaactacc taatgaccat ggtattacct
    24781 tttaacttga ttgaaggtgt tatcttttcc gtttcattct ggttgttgta tgttctcttg
    24841 aaaccaacct taaaacatta tgagagataa acaaacattt ttaatgaagg gcagttttgc
    24901 ccttttactt ttcgttattc ttggctacat ggtcaaattt taccctgaaa cgctggtcaa
    24961 ttttgaccaa tcgattcaga ctgccattcg aggagactta ccagattatt tgactattct
    25021 ttttcgagcc ctcacacgtc tgattgatat cccagtgatt atcacttggg ttgtcattac
    25081 agcttttgtc ttttatcgta agcgatggaa gatagaaagt ttcttcatgc tgggaaatct
    25141 ggctttggca ggtcttttaa tcgtgacctt taaaaatatc taccagcgcc cacggccaga
    25201 tatcttacat ctggtggagg agaagggatt ttccttccca agtgggcatt ctctggctgt
    25261 aaccttgatg gtaggtactt tgattgttat tcttagtcag cggattaaag atccagtctg
    25321 gagaaaaatc gtacaaatcg tccttggcct ctacctagtc agtgtgctgg tatcaagagt
    25381 ctatctggga gttcattacc catcagacgt ccttgccagt ctctgtgtgg gcttgggagt
    25441 cctgtttatc gagtttccct tctatgacaa gctccgcttc caatggcgat ttaaaggcaa
    25501 acagaagtga gtatcgaatt cccttgagga gaaagaaatg aaagtcaaca tagcagatct
    25561 tcatccgact caactatatt tatcagaaaa gaagttgcaa gatattcaga tgctttacca
    25621 gtcggcagaa acaatccaag tcgatccaat cagtattctt gcctttggag attgtttgct
    25681 gattacagat gggcatcaca gggcttatca gactttattg gcagatcggg atacgatttc
    25741 tgctgagtgg gatagagatg gtggtgatga actatatcat ctctatgcgc aagcttgcga
    25801 ggaaagaaag atttactctg tttttgattt agaagatcgt atcctagctc aagatgggta
    25861 tgaagcaaaa tggtataact ggtgtgatgg ttttaatcaa gcagcaactc ttttgttgaa
    25921 aaggtagtgc attgtgtttt gttccttatt ataaggtcaa tcattgtgag gaagcttttg
    25981 cttggtatca ggatgtgaac ttggtttacc tcgtagatgg ggtgaagctt ccttacagtc
    26041 aagcgacctt ggaagccatg tattcctatt tggatcggca tggtgagctt ttttggattg
    26101 aagtcaagga gaagggagaa tggtttccaa ttggggatat tacactatct caggataatc
    26161 tccccattgt gattgggaat tccgcttacc aacatcgagg acttggaaaa aagattctaa
    26221 gtgctttgat tgaattggct cgagtaaaag gatggaaaga attgagagtc aaggaaatct
    26281 acacctacaa tcatgcttct aggaggtgtt tcaagtcgct tggatttgtg gaaaatggag
    26341 caacagaaaa aggaatgagt tttatattgg aattagtcta atcctgccat tttctccccc
    26401 ttaacctttg actattctgc aaaattatcg taaaataaag agtaaatgat aaaatgaggt
    26461 cagagtctgt tcgctctggc gatagtagta taaatgagga gaaacgcttt ggaattagaa
    26521 gtatttgctg ggcaagaaaa aagtgaacta tctatgattg aggtagcgcg tgctatcttg
    26581 gaacttcgtg gtcgcgatca tgagatgcat tttagcgatc ttgtaaacga aattcaaaac
    26641 taccttggaa catcaaacag cgatatccgc gaagctttgc ctttgttcta cacagagttg
    26701 aactttgacg gtagcttcat ctcacttggg gacaacaaat ggggtcttcg ttcatggtat
    26761 ggtgtggacg aaatcgacga agaaatcatc gctcttgaag aaaatgacga cgatgaagta
    26821 gcaccaaaag ctaagaaaaa acgtgttaat gcctttatgg atggtgattc agatgccatt
    26881 gactacaatg cagatgatcc agaagacgaa gatgcatacg aagcagatcc agctctttca
    26941 tacgatgatg aaaatccaga tgatgaaaaa aatgaagtgg aagcttatga tgcagaaatc
    27001 aacgaaatcg ctccagatga cttgggagaa gatgtggatc tcaacgaaga cgacgacgag
    27061 ttttcagatg acgacgctga aaccagcgag gaataagagt tagctattga caattatcct
    27121 atttttaggt atcatattgt ttgggcacct cttttagagg tcggggctcc ctagttctta
    27181 gggagctatt tttgtttttt caagaagtta tcttcttgta ttttatactc aatgaaaatc
    27241 aaagagcaag ctaggaaact agccgtaggc tgctcaaaac actgttttga ggttgtagat
    27301 aagactgaca aagtcaggaa cacatatcta cggcaaggcg acgttgacgc ggtttgaaga
    27361 gattttcgaa gagtattagt tgtgaatctg gtgcagtcgt cccagattat tcttattagt
    27421 agggtcttgt tttctatatc ccctcgtagt taacaagacc ttgagcattt tagaaagagg
    27481 aatctatgtc tacgaaatat atttttgtaa ctggtggtgt ggtatcgtcc attgggaaag
    27541 ggattgtggc agcgagtcta ggccgtctct tgaaaaatcg tggtctcaaa gtaaccattc
    27601 aaaagtttga cccttatatc aatattgatc cgggaaccat gagtccttac cagcacgggg
    27661 aagtttttgt gacagatgac ggagctgaga cagatttgga cttgggtcac tatgaacgtt
    27721 tcatcgatat caatctcaac aaatattcca acgtgacaac tgggaaaatt tacagtgaag
    27781 ttcttcgtaa agaacgccgt ggagaatacc ttggggcaac tgttcaagtc attcctcata
    27841 tcacagatgc tttgaaagaa aaaatcaagc gtgccgctct aacgaccgac tctgatgtca
    27901 ttatcacaga ggttggtgga acagtaggag atatcgagtc cttgccattc ctagaggctc
    27961 ttcgtcagat gaaggcagat gtgggtgcgg ataatgtcat gtatatccat acaaccttgc
    28021 ttccttacct caaggctgct ggtgaaatga aaaccaaacc aacccaacac tctgtcaaag
    28081 aattgcgtgg cttgggaatt caaccaaata tgttggttat tcgtacagaa gagccagctg
    28141 gtcaaggaat taaaaataaa ctggcccagt tctgtgatgt ggcaccagaa gccgttatcg
    28201 aatcgttgga tgttgaacac ctttaccaaa ttccactgaa cttgcaggca caagggatgg
    28261 accaaattgt ttgtgatcat ttgaaattag acgcaccagc agcggatatg acagaatggt
    28321 cagccatggt ggacaaggtc atgaacctca agaaacaagt taagatttcc cttgttggta
    28381 agtatgtgga gttgcaagat gcctatatct cagtggtcga agccttgaaa cactctggct
    28441 atgtcaatga tgtagaagtt aaaatcaatt gggtcaatgc caatgatgtg acagcagaga
    28501 atgtagcaga actcttgtct gatgcggacg ggatcatcgt accaggtggt tttggtcaac
    28561 gtggtacaga agggaaaatc caagccatcc gctatgcgcg tgaaaatgat gttccaatgt
    28621 tgggagtctg cttgggaatg cagttgacat gtatcgagtt tgctcgtcac gttttaggtc
    28681 ttgaaggtgc caattctgca gagcttgcac cagaaacaaa ataccctatc attgatatca
    28741 tgcgtgatca gattgatatt gaggatatgg gtggaaccct tcgtttggga ctttatccgt
    28801 ctaagttgaa acgtggctct aaggctgctg ctgcttatca caatcaagaa gtggtgcaac
    28861 gccgtcaccg tcaccgttat gagtttaata atgccttccg tgagcagttt gaggcagcag
    28921 gttttgtctt ttcaggagtt tctccagaca atcgtttggt agaaatcgtg gaaattcctg
    28981 aaaataaatt ctttgtagct tgtcagtatc accctgaact gtcaagccgt ccaaaccgac
    29041 cagaagaact ctacactgcc tttgttactg cagcagttga gaacagcaat tagcaaaatc
    29101 agaacctttg agaaaaaatc tcagaggttt tttgcatacg atgatattgc agtatatctg
    29161 aggtaggggt cctctgtatg tacctgctac cgttgaaatc aatagcgact ccctcttgcc
    29221 ctgtgctagt gaatggattt atcagtatat tgaaatgaaa taaaatttga acaaattaat
    29281 tcggaaagcc aaatcaattt ctagcaaagt tttaggaact ggattgtata gtgaattgaa
    29341 ataagatgtg aacatctcta tcaggaaagt caaatcgatt tctaacaatg ttttagaaat
    29401 agaggtgtac tattctagtt tcaatatact atatatctgc agaatactct ttcagtttca
    29461 ttcatcctag tcttgcttag gaaaaagtaa aaagccatcc aattatgatg gatagcttct
    29521 tggttcttag atttgttctg caaaaacctt ttcagcaagg ttcatagcgt ggtctgatac
    29581 acgagtgtag tgtgagatga tgtcgataaa gttgacccca gcttgtgttg aacattcgcc
    29641 tttgttgagg cgtttgatgt gggtttttct gagaacacgt tctatcttat tgattgcttc
    29701 atggcgttct acaagactgc gtgctttttc aatatcattg ttttccacac tatccagagc
    29761 atctttgata aagtcactag tttggcggta aacttcctct aattctttca aggcggcatc
    29821 agaaaattca acatttttcc gttgaagata gtcagtcaga ttgagtagag cctccgtgtg
    29881 gtctccaatc cgttccaaat cacgggagga atcaaggata ttggtaagca cttcactttc
    29941 tttttggctg agagcttcgc ttgaaagggc aatgagataa cgtgttaatt gctcatcgat
    30001 ggtgttaatt gcttcttcgg ttttatgccc tttttcagca actttttcat ccaagtcaat
    30061 gatgtactta taggaaaggt caaaggcttt agcagcgtag tttcctaagt gcaagagctc
    30121 ttttttagca tttcctagag cgatagatgg ggcttgtttg atgaaatgtt catcaagata
    30181 taagggttcg tatttgacaa cctcgtcctc tccaggaata atcttggtta caaagtaagc
    30241 cagagctccg ataaatggaa attggacaat ggtgttggta atattaaagg ttccgtgagc
    30301 aaaggcgatg gtcatttccg gtgctagatt tagcgtagct tcaaaccaat gaatcaggac
    30361 agtaaaagga actagaaaaa taacgcagac aactgttccg ataacgttga aggcaacatg
    30421 agctcctgct acccgtttag ctgcaatatt agcccctaaa gaggcaatga tggctgtaat
    30481 ggttgtcccg atattgtcac caaatagaac tggcaaagct ccctgtagat caattagatt
    30541 gccggcgtag aggttttgta aaatcccaat ggtagccgaa gaagcttgaa ttagcaaggt
    30601 caagccagta ccgacaaaga cacccaaaac aggattctta cttagctcaa tcatatagtc
    30661 cttaaagacc tgtaaatcct tgagtggagc cattgcgccg ctcatgagat tgagggcaaa
    30721 aaagatacca ccgacaccaa agaggatgcg tccgatatta ttgactgtcc gatttttcgt
    30781 aaaaaaaaga cagacggcac cgataaagag cataggtagg gcatagttac ctaatttaaa
    30841 accgatgaga aaggatgtga cagttgtccc aatattagca cccatgacaa tcccgatagc
    30901 ctgacgtaag gttaagagac cggcactgac caggccgact gtgataactg ttacaccaga
    30961 actagactga attagagcag tcatcccaat accaaccaga actccaaaga aaggattact
    31021 agtatatttg tcaatgtaaa aacgaaggcg atctccagca gcttgttgta aaccgtctcc
    31081 catggtcttg atgctatata agaatagccc cagaccacct aaaaagtgaa ataaaatttc
    31141 ctgccaatta atggacattt ctttttcctc cgaaaaataa tcgcggaata tctcctattc
    31201 tattttaaag gataaaagta aatctaacaa gtgttaatgt caagtttgta aaagaaaatt
    31261 ataattgata agtgtattat ttttaagagt ttttaacctt attttagatt tgtgcaaaca
    31321 gttattgttt tttgtctaaa aatgttttag gataaaaatg tgattatcat ggttctagag
    31381 tttgataaaa aactacagaa tataaagcgc tttcttgaaa acaacaaaat caatctttta
    31441 ggaggagaaa atgaagaatc cattttttga aagacgttgt cgttacagta ttcgtaagtt
    31501 atcagtagga gcctgctcgc tgatgattgg tgctgtttta tttgctggtc cagccttggc
    31561 tgaagaaact gcagttcctg aaaatagcgg agctaataca gagcttgttt caggagagag
    31621 tgagcattcg accaatgaag ctgataagca gaatgaaggg gaacatgcta gagaaaacaa
    31681 gctagaaaag gcagaaggag tagcgacagc atctgaaact gcttcgccag caagcaatga
    31741 agctgcaact actgaaactg cagaagcagc tagcgcagct aaaccagagg aaaaagcaag
    31801 tgaggtggtt gcagaaacac catctgcaga agcaaaacct aagtctgaca aggaaacaga
    31861 agcaaagccc gaagcaacta accaagggga tgagtctaag ccagcagccg aagctaataa
    31921 gactgaaaaa gaagtccagc cagatgtccc taaaaataca gaaaaaacat taaaaccaaa
    31981 ggaaatcaaa tttaattctt gggaagaatt gttaaaatgg gaaccaggtg ctcgtgaaga
    32041 tgatgctatt aaccgtggat ctgttgtcct cgcttcacgt cggacaggtc atttagtcaa
    32101 tgaaaaagct agcaaggaag caaaagttca agccttatca aacaccaatt ctaaagccaa
    32161 agaccatgct tctgttggtg gagaagagtt caaggcctat gcttttgact attggcaata
    32221 tctagattca atggtcttct gggaaggtct cgtaccaact cctgacgtta ttgatgcagg
    32281 tcaccgtaac ggggttcctg tatacggtac actcttcttc aactggtcta atagtattgc
    32341 agatcaagaa agatttgctg aagctttgaa gcaagacgca gatggtagct tcccaattgc
    32401 ccgtaaattg gtagacatgg ccaagtatta tggctatgat ggctatttca tcaaccaaga
    32461 aacaactgga gatttggtta aacctcttgg agaaaagatg cgccagttta tgctctatag
    32521 caaggaatat gctgctaagg taaaccatcc aatcaagtat tcttggtacg atgccatgac
    32581 ctataactat ggacgttacc atcaagatgg tttgggagaa tacaactacc aattcatgca
    32641 accagaagga gataaggttc cggcagataa cttctttgct aactttaact gggataaggc
    32701 taaaaatgat tacactattg caactgccaa ctggattggt cgtaatcctt atgatgtatt
    32761 tgcaggtttg gaattgcaac agggtggttc ctacaagaca aaggttaagt ggaatgacat
    32821 tttagacgaa aatgggaaat tgcgcctttc tcttggttta tttgccccag ataccattac
    32881 aagtttagga aaaactggtg aagattatca taaaaatgaa gatatcttct ttacaggtta
    32941 tcaaggagac cctactggcc aaaaaccagg tgacaaagat tggtatggta ttgctaacct
    33001 agttgcggac cgtacgccag cggtaggtaa tacttttact acttctttta atacaggtca
    33061 tggtaaaaaa tggttcgtag atggtaaggt ttctaaggat tctgagtgga attatcgttc
    33121 agtatcaggt gttcttccaa catggcgctg gtggcagact tcaacagggg aaaaacttcg
    33181 tgcagaatat gattttacag atgcctataa tggcggaaat tcccttaaat tctctggtga
    33241 tgtagccggt aagacagatc aggatgtgag actttattct actaagttag aagtaactga
    33301 gaagaccaaa cttcgtgttg cccacaaggg aggaaaaggt tctaaagttt atatggcatt
    33361 ctctacaact ccagactaca aattcgatga tgcagatgca tggaaagagc taaccctttc
    33421 tgacaactgg acaaatgaag aatttgatct cagctcacta gcaggtaaaa ccatctatgc
    33481 agtcaaacta tttttcgagc atgaaggtgc tgtaaaagat tatcagttca acctaggaca
    33541 attaactatc tcggacaatc accaagagcc acaatcgccg acaagctttt ctgtagtgaa
    33601 acaatctctt aaaaatgccc aagaagcgga agcagttgtg caatttaaag gcaacaagga
    33661 tgcagatttc tatgaagttt atgaaaaaga tggagacagc tggaaattac taactggctc
    33721 atcttctaca actatttatc taccaaaagt tagccgctca gcaagtgctc agggtacaac
    33781 tcaagaactg aaggttgtag cagtcggtaa aaatggagtt cgttcagaag ctgcaaccac
    33841 aacctttgat tggggtatga ctgtaaaagc taccagccta ccaaaaccac tagctgaaaa
    33901 tatcgttcca ggtgcaacag ttattgatag tactttccct aagactgaag gtggagaagg
    33961 tattgaaggt atgttgaacg gtaccattac tagcttgtca gataaatggt cttcagctca
    34021 gttgagtggt agtgtggata ttcgtttgac caagtcacgt accgttgtta gatgggtcat
    34081 ggatcatgca ggagctggtg gtgagtctgt taacgatggc ttgatgaaca ctaaagactt
    34141 tgacctttat tataaagatg cagatggtga gtggaagcta gctaaggaag tccgtggtaa
    34201 caaagcacac gtgacagata tcactcttga taaaccaatc actgctcagg actggcgctt
    34261 gaatgttgtc acttctgaca atggaactcc atggaaggct attcgtatct ataactggaa
    34321 aatgtatgaa aagcttgata ctgagagtgt caatattccg atggccaagg ctgcagcccg
    34381 ttctctaggc aataacaagg tacaagttgg ctttgcagat gtaccggctg gagcaactat
    34441 taccgtttat gataatccaa attctcaaac tccgctcgca accttgaaga gcgaagttgg
    34501 aggagaccta gcaagtgcac cattggattt gacaaatcaa tctggtcttc tttattatcg
    34561 tacccagttg ccaggcaagg aaattagtaa tgtcctagca gtttccgttc caaaagatga
    34621 cagaagaatc aagtcagtca gcctagaaac aggacctaag aaaacaagct acgccgaagg
    34681 ggaggatttg gaccttagag gtggtgttct tcgagttcag tatgaaggag gaactgagga
    34741 cgaactcatt cgcctaactc acgcaggtgt atcagtatca ggttttgata cgcatcataa
    34801 gggagaacag aatcttactc tccaatattt gggacaacca gtaaatgcta atttgtcagt
    34861 gactgtcact ggccaagatg aagcaagtcc gaaaactatt ttgggaattg aagtaagtca
    34921 ggaaccgaaa aaagattacc tagttggtga tagcttagac ttgtctgaag gacgctttgc
    34981 agtggcttat agcaatgaca ccatggaaga acattccttt actgatgagg gagttgaaat
    35041 ttctggttac gatgctcaaa agactggtcg tcaaaccttg acgcttcgtt accaaggtca
    35101 tgaagtcaac tttgatgttt tggtatctcc aaaagcagca ttgaacgatg agtacctcaa
    35161 acaaaaatta gcagaagttg aagctgctaa gaacaaggtg gtctataact ttgcttcacc
    35221 agaagtaaaa gaagccttct tgaaagcaat tgaagcggcc gaacaagtgt tgaaagacca
    35281 tgaaactagc acccaagatc aagtcaatga ccgacttaat aaattgacag aagctcataa
    35341 agctctgaat ggtcaagaga aatttacgga agaaaagaca gagcttgatc gcttaacagg
    35401 tgaggctcaa gaactcttgg ctgccaaacc aaaccatcct tcaggttctg ccctagctcc
    35461 gcttcttgag aaaaacaagg ccttggttga aaaagtagat ttgagtccag aagagcttgc
    35521 aacagcgaaa cagagtctaa aagatctggt tgctttattg aaagaagaca agtcagcagt
    35581 cttttctgat agtaaaacag gtgttgaagt acacttctca aataaagaga agactgtcat
    35641 caagggtttg aaagtagagc gtgttcaagc aagtgctgaa gagaagaaat actttgctgg
    35701 agaagatgct catgtctttg aaatagaagg tttggatgaa aaaggtcaag atgttgatct
    35761 ctcttatgct tctattgtga aaatcccaat tgaaaaagat aagaaagtta agaaagtatt
    35821 tttcttacct gaaggcaaag aggcagtaga attggctttt gaacaaacgg atagtcatgt
    35881 tatctttaca gcacctcact ttactcatta tgcctttgtt tatgaatctg ctgaaaaacc
    35941 acaacctgct aaaccagcac cacaaaacac agtccttcca aaacctactt atcaaccggc
    36001 ttctgatcaa caaaaggctc ctaaattgga agttcaagag gaaaaggttg cctttcatcg
    36061 tcaagagcat gaaaatgctg agatgctagt tggggaacaa cgagtcatca tacagggacg
    36121 agatggactg ttaagacatg tctttgaagt tgatgaaaac ggtcagcgtc gtcttcgttc
    36181 aacagaagtc atccaagaag cgattccaga aattgttgaa attggaacaa aagtaaaaac
    36241 agtaccagca gtagtagcta cacaggaaaa accagctcaa aatacagcag ttaaatcaga
    36301 agaagcaagc aaacaattgc caaatacagg aacagctgat gctaatgaag ccctaatagc
    36361 aggcttagcc agccttggtc ttgctagttt agccttgacc ttgagacgga aaagagaaga
    36421 taaagattaa atatcgaaaa atcttgtgaa atctttcctt atatttccaa agtgtgatat
    36481 aatagtttcg aataaaataa ataaaggagt ttttgtaaca tggcaaaact tactgttaaa
    36541 gacgttgact tgaaaggtaa aaaagtcctc gttcgtgttg acttcaacgt accattgaaa
    36601 gatggcgtaa tcactaacga taaccgtatc acagcagctc ttccaactat taagtacatc
    36661 atcgaacaag gtggacgtgc aattcttttc tctcaccttg gacgtgtgaa agaagaagct
    36721 gataaagctg gtaaatcact tgctcctgta gcagctgact tggcagcaaa acttggtcaa
    36781 gatgttgttt tcccaggtgt cactcgtggt gctgaattgg aagcggcaat caacgctctt
    36841 gaagatggac aagttctctt ggttgaaaac actcgttacg aagatgttga cggcaagaaa
    36901 gaatctaaaa acgatcctga acttggtaaa tactgggcat cacttggaga tggtatcttc
    36961 gtaaacgatg cattcggtac agctcaccgt gcacacgcat ctaacgttgg tatctcagca
    37021 aacgttgaaa aagcagttgc tggtttcctt cttgaaaacg aaattgccta catccaagaa
    37081 gcagttgaaa ctccagaacg tccattcgtg gctatccttg gtggttcaaa agtttcagac
    37141 aagatcggtg ttatcgaaaa cttgcttgaa aaagctgata aagtccttat cggtggtggg
    37201 atgacctaca cattctacaa agcacaaggt atcgaaatcg gtaactcact tgtagaagaa
    37261 gacaaattgg atgttgcgaa agctcttctt gaaaaagcaa atggtaaatt gatcttgcca
    37321 gttgactcaa aagaagctaa cgcatttgct ggttacactg aagtgcgtga cactgaaggt
    37381 gaagcagttt ctgaaggctt ccttggtctt gacatcggtc caaaatctat cgccaaattt
    37441 gacgaagctt tgactggtgc caaaacagtt gtatggaacg gacctatggg tgtatttgaa
    37501 aacccagatt tccaagctgg tacaatcggt gtgatggacg ctatcgtgaa acaaccagga
    37561 gttaaatcaa tcatcggtgg tggtgactca gctgccgcag cgattaacct tggccgtgca
    37621 gacaagttct catggattag tacgggtggt ggagcatcaa tggaacttct tgaaggtaag
    37681 gttcttccag gacttgcagc cttgacagaa aaataagatt ttataaacaa atcaaagaag
    37741 agagggatga aagttcctct tttcttttgc ttaaaataaa aacgcttcct ctcaactatt
    37801 accccaaatc attcatacct ctctcaacta gatgtaactt acaaaacccc tgacctcatg
    37861 agccactttc ttcctcctca tgaggtcagt tttactttct gctgttccag tatcgttttt
    37921 cctcgctaga tttcctcaaa agggcagact cctcccttgg tgcgtcacac gattttttca
    37981 tctcgactgt tctttaatgc atcattaacg atgcttttct tctaggtggt tcataaggaa
    38041 caggaagatt caggttgact tttctaatcc tagaataaag tgctgaaaac aattcggaat
    38101 aggcatagag actagacaat ttgaggagct gcttgcgtcc tgttcgaaca cattttccta
    38161 ccacgtgaag aaaaagatgg cggaagcgtt tgattgttaa agtttggaag tcacctccag
    38221 ctagatgttt gagaaaaaga tagagattgt aggcgataca gctcatcatc atacgaactt
    38281 cgtttttgat taag
//
LOCUS       DAVKWS010000015        36217 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKWS010000015 DAVKWS010000000
VERSION     DAVKWS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.015"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(81..929)
                     /locus_tag="VW852_000795"
     CDS             complement(81..929)
                     /locus_tag="VW852_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0271969.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1081..1359
                     /locus_tag="VW852_000796"
     CDS             1081..1359
                     /locus_tag="VW852_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271970.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1361..3424
                     /locus_tag="VW852_000797"
     CDS             1361..3424
                     /locus_tag="VW852_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0271971.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3502..4758)
                     /locus_tag="VW852_000798"
     CDS             complement(3502..4758)
                     /locus_tag="VW852_000798"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV0271972.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            4900..7365
                     /locus_tag="VW852_000799"
     CDS             4900..7365
                     /locus_tag="VW852_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0271973.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7430..8341
                     /locus_tag="VW852_000800"
     CDS             7430..8341
                     /locus_tag="VW852_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0271974.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8571..9269
                     /gene="dapD"
                     /locus_tag="VW852_000801"
     CDS             8571..9269
                     /gene="dapD"
                     /locus_tag="VW852_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV0271975.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTTVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9337..10467
                     /locus_tag="VW852_000802"
     CDS             9337..10467
                     /locus_tag="VW852_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV0271976.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLLFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVVASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10479..11018
                     /locus_tag="VW852_000803"
     CDS             10479..11018
                     /locus_tag="VW852_000803"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV0271977.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRTLSTVYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11002..11679
                     /locus_tag="VW852_000804"
     CDS             11002..11679
                     /locus_tag="VW852_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV0271978.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQIWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(11746..12645)
                     /gene="galU"
                     /locus_tag="VW852_000805"
     CDS             complement(11746..12645)
                     /gene="galU"
                     /locus_tag="VW852_000805"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV0271979.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12667..13683)
                     /locus_tag="VW852_000806"
     CDS             complement(12667..13683)
                     /locus_tag="VW852_000806"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0271980.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGANPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            13895..14275
                     /locus_tag="VW852_000807"
     CDS             13895..14275
                     /locus_tag="VW852_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0271981.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(14449..14874)
                     /locus_tag="VW852_000808"
     CDS             complement(14449..14874)
                     /locus_tag="VW852_000808"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0271982.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(14942..15592)
                     /gene="phoU"
                     /locus_tag="VW852_000809"
     CDS             complement(14942..15592)
                     /gene="phoU"
                     /locus_tag="VW852_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0271983.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15607..16359)
                     /gene="pstB"
                     /locus_tag="VW852_000810"
     CDS             complement(15607..16359)
                     /gene="pstB"
                     /locus_tag="VW852_000810"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0271984.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16361..17176)
                     /gene="pstA"
                     /locus_tag="VW852_000811"
     CDS             complement(16361..17176)
                     /gene="pstA"
                     /locus_tag="VW852_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0271985.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17169..18032)
                     /gene="pstC"
                     /locus_tag="VW852_000812"
     CDS             complement(17169..18032)
                     /gene="pstC"
                     /locus_tag="VW852_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0271986.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18150..19025)
                     /locus_tag="VW852_000813"
     CDS             complement(18150..19025)
                     /locus_tag="VW852_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV0271987.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19192..20523)
                     /locus_tag="VW852_000814"
     CDS             complement(19192..20523)
                     /locus_tag="VW852_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV0271988.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20524..21231)
                     /locus_tag="VW852_000815"
     CDS             complement(20524..21231)
                     /locus_tag="VW852_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0271989.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21315..21800)
                     /locus_tag="VW852_000816"
     CDS             complement(21315..21800)
                     /locus_tag="VW852_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV0271990.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            21786..22343
                     /locus_tag="VW852_000817"
                     /pseudo
     CDS             21786..22343
                     /locus_tag="VW852_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22534..24225)
                     /locus_tag="VW852_000818"
     CDS             complement(22534..24225)
                     /locus_tag="VW852_000818"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV0271991.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIATELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQIAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            24361..24807
                     /gene="argR"
                     /locus_tag="VW852_000819"
     CDS             24361..24807
                     /gene="argR"
                     /locus_tag="VW852_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0271992.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            24858..27392
                     /gene="mutS"
                     /locus_tag="VW852_000820"
     CDS             24858..27392
                     /gene="mutS"
                     /locus_tag="VW852_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV0271993.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEHISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            27539..29233
                     /gene="patA"
                     /locus_tag="VW852_000821"
     CDS             27539..29233
                     /gene="patA"
                     /locus_tag="VW852_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV0271994.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(29564..29833)
                     /locus_tag="VW852_000822"
                     /pseudo
     CDS             complement(29564..29833)
                     /locus_tag="VW852_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            30025..31791
                     /gene="patB"
                     /locus_tag="VW852_000823"
     CDS             30025..31791
                     /gene="patB"
                     /locus_tag="VW852_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV0271995.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVSDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFK"
     gene            31930..32619
                     /locus_tag="VW852_000824"
     CDS             31930..32619
                     /locus_tag="VW852_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0271996.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            32623..32802
                     /locus_tag="VW852_000825"
     CDS             32623..32802
                     /locus_tag="VW852_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0271997.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            32789..34138
                     /locus_tag="VW852_000826"
     CDS             32789..34138
                     /locus_tag="VW852_000826"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV0271998.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            34262..35722
                     /locus_tag="VW852_000827"
     CDS             34262..35722
                     /locus_tag="VW852_000827"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV0271999.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            35736..36170
                     /locus_tag="VW852_000828"
     CDS             35736..36170
                     /locus_tag="VW852_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0272000.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     EFNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        10535 a         6962 c         7997 g        10723 t
ORIGIN      
        1 ggttttgagg ttgtagatga aactgacgaa gtcagtaacc atacgtacgg caaggcgacg
       61 ttgacgtggt ttgaagagta ttagaatgct ttcccaacca gaatctctgc ttcgatggta
      121 atctctgtca gttggctcca gtcaatcttg ctctggtcaa cgtgaaagag gagaggagtc
      181 atactgagta gggcttggag ttgttctgct gtgatagtct tagtcagaga ggcagtttga
      241 ctagatagga tggtaaagtg ttcctggaaa tgctccttga tatcttggtt agaatactcc
      301 ttgtttgtca gctggtcctg tactctttga cggatttctt tgaggtgatt ttcagttggg
      361 ataaccttta tcaagatacc gtctttggat aaaacgcgac gaaattctcc atagttggca
      421 ggtgagaaga tatcaagcag aatatccatg ttagcgtctt ttataggaag gcgtgccaag
      481 tcgccaacga accaattgac tgcccagttg ggttcacttt tagcagcgat ttggactgaa
      541 tctttggaga tgtcaaaggc atagaaagtt ttttcagagt gactttcttg tagtttgcga
      601 gaatagaatc cttcaccaca accgatatcc aaaattgttg tggtagtttt tgagcttgca
      661 agcaagtcag atacagcatc taagatagct tggtaaaagc cggcttctag gatttgttga
      721 cggttttgaa aattttcctt gttatagtta gcagattgct tgatttgagg tgctagattg
      781 acatagccaa atttcgccaa gtcaaaagaa tgacggttgc agcacttgaa attagtctct
      841 aacagagtca gattttcttg acagatagga caggcaaagg cagtcgcaga agcaaaacgc
      901 tgaagtttgg gcttgagatt tgtattcata gtttaagtgt atcaaaagag gcaaatgaaa
      961 gcaactttag gaataagtag atgggagatt cagtagaaat aaaactaatt ctccaattta
     1021 tagaatgaaa ttgcttttat atctaaattg ctatataata atgataagga ggaaataagt
     1081 atgttaaaag aagtattaac cgttgcaaaa gttgcgaaaa aatcttcatt atttttgggt
     1141 ggtgtcgcat ttggtaccct tggtttgaaa atcttagcaa gtaaggaagc taaaaaaggt
     1201 tattctaaag ctttggctaa ggcttacaag ttgaaagacg agctagatgc atctgtttct
     1261 gttgtgaagc aacatggaga cgatgtcttg caagatgcca aatatttgta cgagcaagag
     1321 aaaaaagaag agcaattaga tagccttata ggtgaataat atgtctttta aagtgctaca
     1381 tagaggatac caacatatcc gactatcatc ttctttttca cttaccttgg atattcaaga
     1441 ctatcttcgt tccttggcga gagatgaaaa ggggattgag tctatccagt tttacatgga
     1501 tcaacagcac tttactctac gcataaaaga aggcttttct gtattagata atgcagaagc
     1561 ctttttaaaa agaattgata aagggaaagt ttctgagttg atgactcttc ccattcgtag
     1621 agaagagagt gcttattcta ttgtttcagg tgcagcggtt aagcgtgtac tttttcgtag
     1681 ttttgtgccg tatcctattc gctatatatg gacttgttat caggctttgg gttatattag
     1741 agaagcctat caaacactag cgcgtaagga actaacgatg gaagtcttgg actgttcggc
     1801 tattttattg tctttgttta tgaaccaatc caagacagct agcaatatca tgtttatgct
     1861 tgatttgggg aatcatttag atcagtggtc cttgaaaaaa actgcaacag atttagaaca
     1921 gagtcttctt gcaaaagaga gcgatgtatt cctagtacag ggcgatacgg ttgttagtat
     1981 caagagttcc gatgttcaaa taggagatgt cttgatttta tctcaaggaa atgaaattct
     2041 gtttgatgga caagtagttt caggtttagg tatggtcaac gaaagttcct tgacaggaga
     2101 gagttttcca gttgaaaaaa gagagtctga tttggtttgt gcaaatacag tattagaaac
     2161 tggagagtta cgcattcgtg taacagataa tcagatgaac agccgtattt tacagctgat
     2221 tgagttgatg aagaaatctg aagaaaacaa gaaaacgaaa caacgctatt tcatcaagat
     2281 ggcggataaa gtcgtcaaat ataatttctt gggggctggg ctgacttacc tattgacagg
     2341 ttctttttct aaggctattt ctttcctatt ggtcgatttc tcctgcgctt tgaaaatctc
     2401 tactcctgta gcttatttga cagttatcaa ggaggggttg aaccgtgaaa tggtgattaa
     2461 ggatggagat gttctggaga aatatctgga agttgatact ttcttgtttg ataagacagg
     2521 aacaatcaca actagttatc ctatagttga aaaggtgtta ccttttggag actatagtga
     2581 ggaagatatt ctcagaatca gtgcctgtct tgaggaacac atttatcatc ctattgctaa
     2641 tgccatcgtc aagcaagctg agatagaggg aattgaacat gaggaaatgc atgggaaact
     2701 ccaatatatc gcaagcaagg ggatcaaatc tcatatagat ggccaaccag ttcttattgg
     2761 gaattatgtc ttgatgcagg atgagcagat tcatatcagt tcagaacaaa atgctttaat
     2821 tgaagagtac aagagtcact acaatctctt attcttggct tatcaaaatg aattgattgg
     2881 aatgttctgc attcatactc ctttgagaaa agaagcaaaa acagccttgg ataaacttaa
     2941 ggcacagggg aaaaaattga ttctggcaac aggagacacc ttgattagga cagaggaatt
     3001 agtcaaagat ttgccctttg atcaggtcta tacagacttg aaacctgatg ggaaatttga
     3061 gttagtagag aaactgcaga aagcaggtca cactattttg atggttggag atggattgaa
     3121 tgactcagcg gctttaactc tatcagatat cggtgtggtg atgaatgaga gtgcagatat
     3181 ttctaagcag atgagtgata tcttattgtt agataatcgt ttggatttct tccaagagtt
     3241 ggattcgtta tcatcatctt tgcaaacact catcaagaaa aatattcaag ataccgttgt
     3301 cgtaaatagt agtttgattg gctttggctt atttaattgg ttcagtcctt caaatctctc
     3361 tatcttacat aatctaacaa ccttgcgcat tgtcttgcgt agcttgtcta ttaaaaatag
     3421 atagatgaga gttattaaca gcaaaaagga gttacctttc tcgaggttaa ctcctttgtt
     3481 tatagataaa tgttgaatag tttagtaagt caatacaaag tattttttct tcccacgacg
     3541 gataacagtc agttcatttt ctaacttatc agcgtcactc aagacatagt caaggtcttg
     3601 gatgcggtcg ccgtttacgt agatggctcc gttttggacg tcttcacggg cttggcgttt
     3661 tgagttaact ataccagatg agacgagcag ttccacgata ttgttgtttt cgtctgcctg
     3721 tacttggtag tttggcacac cacgaagtcc ttgtttgagc tctttgacag aaaggttttt
     3781 gatgtttcct gcaaagagtt gctcagtgat gttaagtgct tctttgtagg cttcttctcc
     3841 gtgaacaagt gtaacaactt cacgagccaa gactttttga gccaagcgtt cgtgtggcgc
     3901 tgcttcaaat tgtttacgaa tatcttcaat ctcatcaagt gacaagaaag taaagatttt
     3961 caagaagcga acagcgtcag cgtccatcac gttcatccag aattggtaca tttcgtatgg
     4021 agaagtcttt tcgggattga gccagacggc atttccttct gatttaccaa attttttacc
     4081 agttgcatct gtgattagtg gaacagtgat aacgtgacca gtcttgtccg ccttacgacg
     4141 aagcaattcg gtaccagctg tcatatttcc ccactggtca gaaccaccga tttgaagagt
     4201 gacattatgg tcttggttaa ggacgaagaa gtcgtaccct tgcatgattt ggtaagcgaa
     4261 ctcagtgtaa gaaattcctg tttcgatccg ttttttaaca gattccttac tcatcatgta
     4321 gttgaccgtg aagtattttc caatgtcacg gaggaagtca atgaagctga tgctgccaaa
     4381 ccagtcgtag ttgttgacca tgacagcctt gttttcgcca ttttcaaagt caagaaaacg
     4441 agaaagttgt ccttggatag acttgaccca gccatctact gtgtcttttg tttggagact
     4501 acgttcagca tctttgaagg acggatctcc gatgagacct gtagcaccgc caacgagcgc
     4561 ataaggtttg tgacctgcta gttgcaagcg acgacttgtc aagattgcga caaggtggcc
     4621 taggtgaagg ctgtcagcag ttggatcgta gccagtataa taagaaactt gaccttcttc
     4681 tagggcttta cgcaaagctt cttcatcagt cgtttgaaat atcaaaccac gctcttttag
     4741 ctcatcaaaa atgtgcatgt gtcttttctc ctttttaaaa ttattgtttc tacctattgt
     4801 atcacaaact tgggcaatag cctagcagaa tagggaagaa agtggctatt ttattgaaaa
     4861 tttccctttt gtggtataat agataaagtg aggaaatcca tgcaaaatca attaaatgaa
     4921 ttaaaacgaa aaatgctgga atttttccag caaaaacaaa aaaataaaaa atcagctaga
     4981 cctggcaaga aaggttcaag taccaaaaaa tctaaaacct tagataagtc agccattttc
     5041 ccagctattt tactgagtat aaaagcctta tttaacttac tctttgtact cggttttcta
     5101 ggaggaatgt tgggagttgg gattgctttg gggtacggag tggccttatt tgacaaggtt
     5161 cgggtgcctc agacagaaga attggtgaat caggtcaagg acatctcttc tatttcagag
     5221 attacctatt cggacgggac ggtgattgct tccatagaga gtgatttgtt gcgcacttct
     5281 atctcatctg agcaaatttc ggaaaatctg aagaaggcta tcattgcgac agaagatgaa
     5341 cactttaaag aacataaggg tgtagtaccc aaggcggtga ttcgtgcgac cttggggaaa
     5401 tttgtaggtt tgggttcctc tagtgggggt tcaaccttga cccagcaact aattaaacag
     5461 caggttgttg gggatgcgcc gaccttggct cgtaaggcgg cagagattgt ggatgctctt
     5521 gccttggaac gcgccatgaa taaagatgag attttaacga cctatctcaa tgtggctccc
     5581 tttggccgaa ataataaggg acagaatatt gcaggggctc ggcaagcagc tgagggaatt
     5641 ttcggtgtag atgccagtca gttgactgtt cctcaagcag catttttagc aggacttcca
     5701 cagagtccca ttacttactc tccttatgaa aatactgggg agttgaagag tgatgaagac
     5761 ctagaaattg gcttaagacg ggctaaggca gttctttaca gtatgtatcg tacaggtgca
     5821 ttaagcaaag acgagtattc tcagtacaag gattatgacc ttaaacagga ctttttacca
     5881 tcgggcacgg ttacaggaat ttcacgagac tatttatact ttacaacttt ggcagaagct
     5941 caagaacgta tgtatgacta tctagctcag agagacaatg tctccgctaa ggagttgaaa
     6001 aatgaggcaa ctcagaagtt ttatcgagat ttggcagcca aggaaattga aaatggtggt
     6061 tataagatta ctactaccat agatcagaaa attcattctg ccatgcaaag tgcggttgct
     6121 gattatggct atcttttaga cgatggaaca ggtcgtgtag aagtagggaa tgtcttgatg
     6181 gacaaccaaa caggtgctat tctaggcttt gtaggtggtc gtaattatca agaaaatcaa
     6241 aataatcatg cctttgatac caaacgttcg ccagcttcta ctaccaagcc cttgctggcc
     6301 tacggtattg ctattgacca gggcttgatg ggaagtgaaa cgattctatc taactatcca
     6361 acaaactttg ctaatggcaa tccgattatg tatgctaata gcaagggaac gggaatgatg
     6421 accttgggag aagctctgaa ctattcatgg aatatccctg cttactggac ctatcgtatg
     6481 ctccgtgaaa agggtgttga tgtcaagggt tatatggaaa agatgggtta cgagattcct
     6541 gagtacggta ttgagagctt gccaatgggt ggtggtattg aagtcacagt tgcccagcat
     6601 accaatggct atcagacctt agctaataat ggagtttatc atcagaagca tgtgatttca
     6661 aagattgaag cagcagatgg tagagtggtg tatgagtatc aggataaacc ggttcaagtc
     6721 tattcaaaag ctactgcgac gattatgcag ggattgctac gagaagttct atcctctcgt
     6781 gtgacaacaa ccttcaagtc taacctgact tctttaaatc ctactctggc taatgcagat
     6841 tggattggga agactggtac aaccaaccaa gacgaaaata tgtggctcat gctttcgaca
     6901 cctagattaa ccctaggtgg ctggattggg catgatgata atcattcatt gtcacgtaga
     6961 gcaggttatt ctaataactc taattacatg gctcatctgg taaatgcgat tcagcaagct
     7021 tccccaagca tttgggggaa cgagcgcttt gctttagatc ctagtgtagt gaaatcggaa
     7081 gtcttgaaat caacaggtca aaaaccaggg aaggtttctg ttgaaggaaa agaagtagag
     7141 gtcacaggtt cgactgttac cagctattgg gctaataagt caggagcgcc agcgacaagt
     7201 tatcgctttg ctattggcgg aagtgatgcg gattatcaga atgcttggtc tagtattgtg
     7261 gggagtctac caactccatc cagctccagc agttcaagta gtagttctag cgatagcagt
     7321 aactcaagta ctacacgacc ttcttcttca agggcgagac gataattctc taaatgaagt
     7381 ggccaatcaa tggattgcca cttttttcct ttcgtgttac aataagggta tgaatcagta
     7441 tcagaaaaag attgttaacg gaaaaattta ttcgctccta tccggcttaa tatggggaat
     7501 ctgtggaatt ttaggagagt acttctttac tcattatcag gtgtcttcgg gctggattac
     7561 ctctatgcgt ttgacactgg cagggagtct tgtactcatt tggtctgcaa tacaattaaa
     7621 atcgcaagtg ctagatattt ggcgagacaa gaaaaattac ctgccctttt tagcctatgc
     7681 tattttgggg attttttcag ttcagtattt tttctatctc tgtgtagaat actcaaatgc
     7741 tacgacagca actattttac agtttattag ccctgtcttt atcctctttt acaatcgctt
     7801 ggtttatcaa aaacgagcgt caaaaagcgc tgttttctat gttttggttg ccatgctggg
     7861 tgtttgcttg atggcgacaa agggagatct ctctcagtta tccatgacgc cgctagctct
     7921 tataacaggt ttgctgagtg ccatgggtgt tatgtttaat gttatcttgc cccaaccttt
     7981 tgctaagcgt tatggttttg ttcctacggt tgggtggggg atgattttgg caggtttgtt
     8041 tagcaatgtc ctctcgccgg tttatcagct ttcctttact cttgatattt ggagtatctt
     8101 gatttgcctc attatcgctt tctttggaac ggcttttgct tttttcattt ccatgaaggc
     8161 tgtgtccttg gtttctcctt tggtggttgc cgttatcagt gccagtgaac ctctctcttc
     8221 tgctctcttg agtgttttgt tcttaggatt agtagtggat tggtccctcc ttctagctat
     8281 agccttgatt attttaccca tgatattttt gtctatagaa gaagcgaaag aaagtagata
     8341 aaaagtcttt tcttaattct aaaagtgtgc tatactagaa tagtcaataa aacacgggga
     8401 gatagttgtg aagagtgttt ttaggttgta tcggtagggg cttgctttta ccgacagcac
     8461 gttttatctc acatccctaa aaaatcatac ctaaaaacag acaaaaaggt ttcaaatttg
     8521 aggccttttt tggtagaata ggtatcatta taacgaacca ggaggcacct atgactgcta
     8581 caaaaatgaa cgctcaagaa attatccaat ttatcgccaa tgctgaaaag aaaaccactg
     8641 tcaaagtaac ctttgagggg caactcgcaa ctgctgtgcc tagctctgtt gtcaaactag
     8701 gaaatgtcct attcggagac tggaaggacg tggctccgct tcttgaaggt ttggtagaaa
     8761 atcaagatta tgttgtcgag caagatgctc gtaattctgc agttcctttg ctagataagc
     8821 gtgctatcaa cgctcgtatc gagccaggtg ctattatccg tgaccaggtg gaaattggtg
     8881 acaatgctgt tatcatgatg ggagctgtta tcaatatcgg tgctgaaatc ggtgctggaa
     8941 ccatgattga catgggtgcc atccttggtg gccgtgccat cgttggaaaa aatagccacg
     9001 ttggtgcagg tgcagttttg gcaggtgtga ttgagccagc tagtgctgaa ccagtccgtg
     9061 tcggagacaa tgttcttatc ggtgctaatg cagtggttat cgaaggagtc caaatcggta
     9121 gtggttcagt tgtcgcagca ggagctattg ttacccaaga tgtcccagaa aacgtggtag
     9181 tagcaggtgt tccagctcgt attatcaaag aaattgatgc ccaaactcaa caaaaaacag
     9241 cgctagagga tgcgcttcgt accttgtaat tgtaaaagta aaaaagaggc ggaacccttt
     9301 ttccagcctc tttctgctat atcggaggac agatagatgt tagatttgat tcagactaga
     9361 cgagatttac accagattcc agagattggc ttggaggagt tcaagactca ggcttatttg
     9421 ctggatgtga ttgagaaatt gactacgggc aaggattttg ttcaaattcg tacttggcgg
     9481 acagggattt tggtctacct gcagggaagt cagccggagc gaaccattgg ttggcggaca
     9541 gatattgatg gcctgcctat cgtcgaacaa acaggtctac tttttgcttc tcaacaccaa
     9601 ggtcgcatgc atgcctgtgg tcatgatttt catatgacca ttgccttagg ctgtcttgag
     9661 cgcgctcttg aggagcaacc caagaataat ctgctctttc tatttcagcc tgctgaagaa
     9721 aatgaagctg gtggtatgct catgtatgag gacggtgctt ttggagattg gttgccagat
     9781 caattctatg gtctccatgt tcgtccagat ttgaaagtcg gccagattgc gactaatact
     9841 catacactct ttgcaggaac ttgcgaggtg aagatccgtt tcaaaggcaa aggtggccac
     9901 gcagcttttc ctcatgaagc caatgacgcc ttggtggtgg ctagttactt tgtgacccaa
     9961 gtgcagtcag ttgttagccg caatgtcaac ccaatcgagg gagcagtggt gaccttcggc
    10021 gtttttcaag ctggaaccac caacaatgtc atcacagaca cagctttttt acatggaact
    10081 attcgtgcct tgacacagga catgagtctc ttggtgcaaa agagggtaaa aacagttgca
    10141 gaaggggttg cagctgcctt tgatatggaa gtcgaagtgg aactcaaaca aggtggatac
    10201 ctacctgttg agaacaatcc agccttggcg cgtgaactga tggacttctt tgacgaaaaa
    10261 gacggaatcg agttgattga tatcgaacct gctatgacag gcgaggactt tggttatctc
    10321 ctttcaaagg ttgatggcgt gatgttttgg ctaggtatcg atagtcccta cgctcttcat
    10381 caccctcaaa tgagtcctaa ggaagaagtc ttagctattg gggtggctgc ggtgtctagt
    10441 ttcctgaaaa agaaggcagc agaatagagg gcttgtatat gaaatcggaa ttacgcaagc
    10501 aagtcttgca tgaaatgaag gctttatctc aagaacaaaa acaggctata gaccaagctt
    10561 taaccgagcg aattttacaa cacccctttt atcaagaagc caaggtcatc gcaacctacc
    10621 tctctttttc tcatgagttt caaacgcggg aactgattga gcaggcgctg aaggacggca
    10681 agaaggtttt aatacccaaa acttatccca aggggcgcat ggagtttgtg gtctatgatc
    10741 cgcaacagtt ggtaaaaact tcctttgcct tactggagcc acagggagat ttggaagtgg
    10801 tggatgtatc tcagattgat ttgattcatg ttcctggcct ggcttttacg acggatggat
    10861 atcggattgg atatggtgga ggttattatg accgctatct ggaacatttt tctggtcgta
    10921 ctttgagtac ggtttatcct tgtcaaattc aggactttat acctgaaaac catgatattc
    10981 ctgttcagga ggtattaatt gatgaaggaa atctttgata gacgttaccc tgtgacgagt
    11041 ttcttcctct tagtgacggc cttggtattt ttactaatgt tggtcactgc aggcggaaac
    11101 tttgacaggg cagatacatt atttcgattt ggagccatgt atgggccagc tattcgcctc
    11161 tttcccgagc agatttggcg tctcttgtct gccatttttg ttcatattgg gtgggaacat
    11221 ttcattgtta atatgctttc actttattat cttggaaggc aggtagagga gattttcggt
    11281 tctaagcagt ttttctttct ctatctttta tcaggaatga tgggcaatct ctttgttttt
    11341 gtatttagtc ctaaatcctt agcagcaggc gcctctacct ctctttatgg gctatttgcc
    11401 gcgattatca ttcttcgcta tgcaacgcgt aatccttata ttcagcagtt gggccaatcc
    11461 tatctgacac tttttgtggt taacattatt ggaagtgttc tgattccagg aatcagccta
    11521 gcaggccata tcggtggtgc agttggtggc gcatttctag cagttatctt tccagttaga
    11581 ggagaaaaac ggatgtataa caccagccag agattaggag cggtagtctt gttcgtagga
    11641 ctcgccattt tgcttttcta caagggaatg ggaatgtgac gaagttgaaa gaaaatttgt
    11701 gtaatgaaaa aagataaggc attttttgaa ccttatcttt ttattttatt ccttctcagt
    11761 caattctttt ccaagttgga tgaggtaatt cttcaaatca tctttgactt gtgggtgttt
    11821 gagggcgtag tcgatggatg ttttcatgaa gccaaacttg tctccgacat cgtaacgagc
    11881 ccctttgaac tcacgagcaa atacacgttg tgttttattg agggtgtcga ttgcatctgt
    11941 cagctgaatt tcatttcctg cacctggagc ttgcttttcg agaatctcaa aaatttcagg
    12001 cgtgaggagg tagcgtccga taatagcaag gtcgctagga gcgtcctctg gagctggttt
    12061 ttcaacaaag gtttcaacac tgtaaagacc atcttttcct tcgccttgcg gagcaataac
    12121 cccgtaagca gatacttcgt catgagggac tggcatgaca gcgatagtag acgcgtgggt
    12181 acgctcgtag tcatccatga gttgtttggt aagtggaaca gccttttcgt ctgtgatatc
    12241 catcaagtca tcaccaagca taacgacaaa aggttcattt ccgacgaaag ccttggcttg
    12301 caaaacagca tctccgagac cgcgtggatg agtttggcgg ataaaatgca gacgcatgcc
    12361 agttgtttca tcaactagct tcaaaagatc tgttttccct ttttctttga ggttatattc
    12421 caattcgaag tttgaatcga agtggtcctc aatagaacgt tttgatttac cagtgacaac
    12481 tagaatatct tcaatacctg atttgagagc ctcttccacg ataaactgga tagttggttt
    12541 gtctacgatt ggcaacattt ctttggcaag ggccttggtt gctggtaaaa atcgagttcc
    12601 tagtccagca gcagggataa ctgcctttct aacttttgat gtcataaaaa tcctttctat
    12661 agagggttaa gaccactcat tttctgcttt aaattcattg ttcatgatgt cataaatggc
    12721 atctttgata ttggttccgt gataaataac ttggtaaata gcctgtgtaa tgggcatata
    12781 gactccaagt tcttgggcta gttcataggc tgctcgagtc gttgaaattc cttcgattac
    12841 catgcccata ttagcttcta tatcagctag ggattctcct cgtccgagag catctccagc
    12901 tctccagtta cgagagtgga tggaagttcc cgttacgatc aaatctccca caccagataa
    12961 gccgctatag gtcaatggat tggccccgag tgctacccct aggcgggtga tttctgctaa
    13021 acctcgagcg atgatggctg ccttagcatt atcaccaaat ccaagaccat gtaaagctcc
    13081 agcaccgaca gcaataatat ttttaagagc accagcagtt tcaaccccga taacatccgt
    13141 attggtataa agtcggaagt agtgattact aaatagctcc tgaacgtatt gagctgtttg
    13201 taaatcttta gaagcagcag ttattaaagt taggtcacgc acaatggtct cttctgcatg
    13261 actaggccct gaaacaacga cgatatcact acggagatgt tcaggaattt cttcttcaag
    13321 aatggttgat aatcgtttat ggctatcagg ttctaatccc tttgatgcgt gcatgatgat
    13381 aaccttatgg tccaaggttt gtgcaacttg ctgggcaaca agtcgtgtca cttttgttgg
    13441 gacaacaaac aaaatcgcat ccacatcttt caatgtttct gctaagtcgg tgtaggcaat
    13501 gatattttcg tctagaacga catctttaaa gtagtgctta ttagtatggt gtgtattaat
    13561 ttcattgatt tgctcgggaa gatttcccca aatacgtacc tcgtgtccat tgtcatttaa
    13621 gacttgtgaa agggcggttc cccaagaacc aggccccaag acggcgacgg tttgtttttc
    13681 catgatttcc tccttgatag agttctttct cttattctat catattctag gggattttgc
    13741 acttgcatta ctggggtatg gtggaaaaat acacaaaaac cgagacgaag ggataaactc
    13801 ttagtcttta gttaaagtga gaagaaatct gatagatagt ttgattcagt atatgcttat
    13861 aatagaggta aacaactcag gaggttcttg taggttgcga gagtttggcg aaaaaattaa
    13921 aagattacgt ttggctaaaa aaatcagtcg ttcagaattt tgtggtgatg agtctgaatt
    13981 aagtatccgt caattaatta gaattgaaaa tggagaatcc agaccaacac taacaaagtt
    14041 aaaatatatc gctgaacgtt tgggggttga ggattacaag ttgatgccaa gttatataga
    14101 gttggataag gaatacctag aattgaagta tttcttgatg aggactccta catacgaaga
    14161 tgaaactatc gcccaaaaga aagagagtgt tttgataaga tttttgaaga gtattatgat
    14221 aggctacctg aggaagaaag atttatcatc ccaaattatt catatctggc actaacgaac
    14281 tacacagttc aaaaattacc agaaaagcta gttgaaatac tgtccttctg gtgattttgc
    14341 ttaatttctg atgttagaga cataattgag gagatttgtc ttgatttttt cctaaaaaag
    14401 ggcagactcc tcccttggtt tgatgctcga tttttaaaat aaggtgactt attctgttag
    14461 gcatgttttc ccctgccctc tagctctata tggaacaggg agtatcagat ttatctggca
    14521 gattgtatca aataaggctt gaaactgttt tgaataggca tatagacttg agaatttgag
    14581 aatatgtcgt ctagcagtag agacatattt tccggcaata tgaaggaaga gacgtcgaaa
    14641 acgcttgata gtcaaggcct ttacttcatc accagctagc tgctttaaaa agaggtagag
    14701 attataagcc agacagccca tcatcatacg tacttcattc ttaatcatgg tcgaactatc
    14761 tgtcttatcc ccaaagaatc ctgctttcct ttctttgata aagttttcag cattccctcg
    14821 ttcacgatag aattgaaaca gctcctcact atctacactg cttaaattgg tcaaatgaac
    14881 ttttttacaa ccataaaaaa gagcacttaa tcgtactctt ctcttttaag gattagttga
    14941 attaattcaa atccactagt tctcctgttt ctaggtagac taggcgttca caaatgttag
    15001 caatgtaatc agcgaagcgt tccagatgcc ctatgatata aaggtattgt gttccattgg
    15061 gaattgaggt ttcttggtct ttcataagtc caatgatttc ctttgataag gcataataat
    15121 attggtcaat ctgttcatct ttttgagcaa tactaatagc ttttgaggct tggtgcaaag
    15181 gaaaggcaac caataaatcg gatagcatgc tgagggataa tttacccatt tggtgtaact
    15241 gttcttcgtc aggggctagt tgattttctt ttagttgcaa aacagcttta gcaatgcctg
    15301 ccatatggtc tcccatacgt tcaaggtctg aacaagaaga catgatgcta atcacaaatc
    15361 gaaggtcaga cacttgtggc tgctgcaagg ccaacaaacg ggcacaggtc aattcgatag
    15421 cgctttgacc ttggttgata gcatgatcct tattgataat tagctctgcc atctccttgt
    15481 ctttggaggc taaggccagt aaggtttttg aagctgtttc aaggacaagt tgccctagtc
    15541 ctaaaaagga ttgttctaat tcatgcaatt ctaagtcaaa ttgatttctc ataggttttt
    15601 ccttccttat ccgaaccgtc ctgaaatata gtcttctgtg cgctgatctt ttggattggt
    15661 aaacacgtca acggtgtctc caaattcgca aatttctcct gttaagaaaa aagcagtttt
    15721 atctgaaata cgtgaagctt gttgcatgtt atgggtaaca atgataatcg tataatcctt
    15781 ttttagttgc tgaatgaggt cttcaatttt taaagtggag atagggtcta aggctgaagt
    15841 cggctcatcc attaacagaa catcaggttc tactgctaaa gctcgcgcaa tgcaaaggcg
    15901 ttgctgctga ccgcctgata aggacatggc actcttttta agatcatctt tgacttcgtc
    15961 ccaaatggct gcccctttta aagatttttc cactaaggca tctaattgtt ttttgtctcg
    16021 aataccatgt gtccttgggc cataagccac gttatcatag atagacatgg caaagggatt
    16081 aggctgttga aaaaccatcc ctacacgctt gcgtagctga ttaaggttga atttgctact
    16141 ataaatatct tgctcatcta agaggacttg gccttcaata tggcaagaag gaaccaaatc
    16201 gttcatccga ttaagggttt ttagaaaagt tgatttacca caaccagatg ggcctatcaa
    16261 ggcagtaatc tgtctttctg gtaattgaat cgaaatattt tttaaggctt gaaaatcccc
    16321 gtaaaataag tctaggtgtc tgactgaaaa tgttcccata ctaggaagct cctttcacaa
    16381 gtttccgaga taataagctt gatagagtat ttatcattaa aacagtaata atcaaaatca
    16441 cgccggtagc ataggcttca ttgacatgta gcccctcact tgacagcata tacatatgta
    16501 gggctagaga acggcctgaa gacatgagac tacttggcgt attggtagag gtacctaatg
    16561 tatacatgag ggcagctgtt tcaccaacga tacggccaat agctagtatc actccagcta
    16621 aaatacctgg catggcaact ggtagaacaa ttctaaaaac agtccgtaac ttacctgccc
    16681 caagtccata acttgcttga cgcatgctat cactaacaga taaaagggct tcttctgttg
    16741 agcgaataat gactggcaac accatgataa ctgaggttaa gattcctgat aacagagagt
    16801 attgaaaacc taagaagact acaaagaaga gcatgccaaa cagaccaaaa acaatggaag
    16861 gaatcccaga taaggtatct gaggccaatc gcatgatttt aacacaaagg gaatcttttt
    16921 ttgtatattc cacaagataa aaaccagcaa aaatccctat gggcaaggct aaaagaagag
    16981 caccaaagac cagaataacg gtggaaataa tcgctggcat aagggaaatg ttctcagaag
    17041 tataagtcca agaaaagagg gatagactta gatgaggtaa gcctttgatg aggataaaac
    17101 caatgattaa aaagagagag ccaaaggtta aagctgaaaa acaataaacg agaagtttta
    17161 gcaggtattt actcataaga tgattttcct ttcaagtagg caaagtaggc attaatcaag
    17221 agaataagga aaaagagaac tgctgaggtt gcaataaggg cttccctatg ctgacctgat
    17281 gcgtaagcca tttccagaac aatattggtt gttaaggttc tggttcctga aaagagtcca
    17341 cttggaataa tcggctggtt gcctgccacc aaaataactg ccatggtttc acctactgcg
    17401 cgaccgattc ctaaaataac tgctgataaa ataccagatc tcgcagctgg caagatgaca
    17461 ctaaaaatac tccgttcatg actagctcct agagccaagc taccagaata atacgttttg
    17521 ggaactgttc ggatagcaga ttctgacaaa ctgataatgg ttggcaaaat cattattcct
    17581 aatagtaacg aagcggttag gacactcatg ccatttccta aaaagcttct aatccaaggc
    17641 accaataatt gtaggccgaa aaaaccataa acaatagatg gaatggccgc catcaagttg
    17701 atagctgatt ttaagaagcc atagacgggc tttggacaat aataaaccat aaacaccgat
    17761 gtcaagatgc ctgttggcac cccaatcaca atcgctccta aggtaattaa taaggaacca
    17821 acgatcattg gtaaaatacc atagcttgcc ggaatgttcg ttggcgacca atcactgcct
    17881 aataaaaaac gggcaaagcc gtagttagcc atgaaaggta agccattact aaaaataaag
    17941 aaacagatta gcaaaatagc tacaacagct actgttgcac tcatgaaaaa aattgcccta
    18001 aaaactgctt ctttgaaggc ttgttttatc acatcttgtc ctttctagtg aagaaagtaa
    18061 gggagatacg acacctccct acttgccttc tttatcttat tgtacgatga aacgtctgca
    18121 tctctttaga gatttatgga gcaaacattt tatttaatct tgtcccaggt ggttaatttg
    18181 ccactaaaaa cgtctgcaag ttcagccata ctgacttggc ttgccttatt gtcattattg
    18241 accacaacag caataccgtc taaagcaata gcatcatggg tgagactctt accttcttca
    18301 ggagttaatt ccctagaaac cataccaata tcagcggttt tctccttaac agcggtaata
    18361 cctgctgaag acccattaga ggtaatatca atcgtaactt ctggattttc ttttttataa
    18421 gcttctgcta atttttccat taaagaagat actgaagtgg aacctacaac agacaacttg
    18481 cctgataagt gttggcttgt atattctgtg gtttcggttt tagcttcaat aaatttatta
    18541 tctgtgacca cttgttgacc ttgtttggag tggataaagc tgataaaatc ttgacctagc
    18601 ttggaaagat tagaagacca aacaatgttg aagggacgtt gaagagggta ttcaccatct
    18661 aaaactgtgt ctcgactagc cttgacacca tcaatctcta aagccttgac agatttcgtt
    18721 aaagatccca aggagatgta gccgatagca ttagcattcc cttgaactgc tgagagaaca
    18781 ccttctgtac tattttgaat cacagctgtt ttggcagtgt tgtcaatttt tttatcaccg
    18841 tcttttttga gaatccctgt gatttctgtg aaggcacccc gtgttccaga gccattttct
    18901 cgtgaaatca cctcaatcgt tcctgaagct gactgtttgg aagcagctga ctgattgcca
    18961 caggcaacaa gcccaaatcc tgataagcca atggctgcaa gagtaagcat ttttttgaat
    19021 ttcataataa tcacctttat ctctatgtat ttttcttgtg taggcttact acatttatag
    19081 tctaacaagt ctttgtaaag gtttatccct gattcatgta aagattgtgt aaagaatcaa
    19141 aaaaagccac ttttgaaaaa tggctgctcc taaaaatagc tttaaaaatt attagtcctg
    19201 tgcgaaagat tggttaggaa gaaaaatcgt gaagcaactg cctctgccaa gctgactcgt
    19261 caccgtgact tggccaccta ataattgact gagttctttg acaatggcaa ggccaagacc
    19321 agtgccacca gtttgtctgc ttcgaccttt attaactcgg taaaaacgtt caaaaatacg
    19381 atcctgctct aattgactaa taccaatccc tgtatctgat acagaaatct taatgccttc
    19441 gttcaccttt tgggtcttga cctcaatttt ccccccttgt tcagtgtaac ggatggcatt
    19501 ggataaaaga ttgagtaaga tttgggaaag taattgacta tctgatacga gggtgacatc
    19561 atctggcacc tgcaccttta gctgtaaatc cttcttcttg agctgaggtt gcaagctttg
    19621 agtcaaatcc tgtacaaatt ctgccaaaga aagggtcgtc cattgtatag gcatttgttg
    19681 agccttagat aaggtaagaa gatgctcaac aatatgctca agacgcaaac tttctttgta
    19741 aataatgtct agaaagtcat ccttgagcgc ttcttcttca gctgacatcc ccttaatggt
    19801 ttcagcaaag cccttaatcg aagtaactgg tgtcctcaat tcatgggagg catttgagac
    19861 aaaggctaaa tttaactttt cataagttct aatcgttgtt aaatcatata gcaagacgag
    19921 cacagcttcc acagattggg tggggctaaa aacgggaact gctgtcactt ctaaaatcaa
    19981 gtcaccctca tgaaacccac ttacttcttg ttttaacctt gttttttgat caaaggcttg
    20041 gtgaactaaa ttccgaatat ccatccgttt gaggtcatca agtgaactta tgtcgccgtc
    20101 cacatcggga aaataatgag gcagagagcg actggataat aacatctgac cttgagcgga
    20161 aactaaaaac gtccccatgg ttaggtgtga cagaagaacc tccattgttt cggctagatc
    20221 cttgtattgc tgatcctgtt gggagacttt ggtttttagg ccagacacat actgagccaa
    20281 agactttaag tcttcttgcc ctttttctaa aaagtattca ctactggtca agagaggttg
    20341 gtgcaaggtc tcaaaagcaa cttcccattt ccaaaggcaa aagagccagt agccacctag
    20401 tcccaaagaa agggctagaa gaaagagacc gatgccttta ctgatccaag ttaatgccat
    20461 ccctgcaatc agaatgaggc taacacttag attgactagc caaaattgaa ggtagcgttt
    20521 catctataac tccttgaact tataaccata accccgaatg gttcgaataa attgaggggc
    20581 tttaggattg tcttcaattt tttccctcaa cttaccaata tgaacgtcca ccaaacgtgt
    20641 ttcctgccca aagtcatacc cccagatacg ttccaaaaga cgctctctag tcagtgtcat
    20701 gttgggatgt ttcataagat agagcaagag ttcaaattct tttggggtca aactcagtaa
    20761 cttattcgcc ttgtagactt catgacgctc agggtatact ttcaaggtcc caaatagcca
    20821 agaatcgtca gcgatattat ctgaatcatc tccttcttgt tctcctttag ttcgcctgag
    20881 gacagccttg acacgcgcca gcaattctct agggctaaaa ggcttggtca ggtagtcatc
    20941 agcccctaat tccaaggcca aaaccttatc aaattcatca cttttcgcag aaaccatcat
    21001 aattggagtt ttgacgcctt tggctctcag ccgcttacaa acttccatgc catctaattg
    21061 tggtaacatg atatcaagca agataaaatc aaagggttct gtttctgcca aagctaaggc
    21121 cttccgtcca tttgtcacca attgagtaga aaagccttcc ttacttaaat ggtagtcaag
    21181 caatttcaga atgtgttctt catcatccac taataagact tgttttgtca tctattatct
    21241 cctattggta acattataac acaattatca gaaatcctaa cattgctaaa tcagattaaa
    21301 tttgcctatc aagactagta tctggtcaaa cgctcaatca tctccttgtg ctctggatag
    21361 gtcgccagta gatctaccct ttcaaataat tcaaaatcct caaattcaaa accaggagca
    21421 acaagacaag aaaccagagc atcatcctta tcaactgttg atccccaaat agtgccctta
    21481 ggaacacagt agtgaagttg ttgccctttg gatatgtcca ggcctaaagt gactgcttcg
    21541 tagtgaccat ctgctgtaat catgtgaaca gtaagtgggg atcctgcatg aaaataccag
    21601 atttcatctg ctgtcaatcg gtgaaaatgt gaaggattcg tttcttctaa taagaaataa
    21661 atactggtat aaagcgccct tcccttacca gcaaggttta tagtgtctga agcttttttt
    21721 gtttgtctaa aatagccacc ttcaacatgg ggagctaact ctagagttct tatcaagtct
    21781 tctttatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt gttacgattt
    21841 caagaactcc tctcagctct gaggacacgg taatgattga tgcgacggaa gtaaaaatca
    21901 atcgccctaa aaaacaatta gcgaatgatt ctggtaaaaa gaaatttcac gttatgaagg
    21961 ctcaagcgat tgtcacaagt caagggagaa ttgtttcttt ggatatcact gtgaactatt
    22021 gtcatgatat gaagttgttc aaaatgagtc gcagaaatat cggacaagct ggtaaaatct
    22081 tgactgacag tggttatcaa gggctcatga agatatatcc tcaagcacaa actccacgta
    22141 aatccagcaa actcaagccg ctaacagctg aagataaagc ctgtaaccat gcgctatcca
    22201 aggagagaag caaggttgag aacatctttg ccaaagtaga aacgtttaaa atgttttcaa
    22261 caacctatcg aaatcatcgt aaacgctttg gattacgaat gaatttgatt gctggtatta
    22321 tcaatcatga actaggattc tagttttgca ggaagtctat tatttggtta ggtgaattag
    22381 tgaagcgttt aggcaagtgt ctctggttac gacgtcatgg cctctaaatc gattatattt
    22441 aggggtcatg actagtgaag cgcctagcca aagtccgaat aggatttggc gttagttact
    22501 tagattgctt tgcaatcaag taactttggc gatttacatc ttctctggcg cttctactcc
    22561 aagcaagcga agggcttctt tgagaacgac tgcggttgcg tagctgaggg ctagacggct
    22621 gtcgcgttct gggctttcat ccaagatacg tgtatgtgca tagtatttgt taaaggattg
    22681 agccaggcta attgcaaatt tagcaatgat agaaggttca aagttatctg ccgcacggtt
    22741 gataatacgt gggaagtctt gaatgagttt aataatttcc cagctttcag tatcattcaa
    22801 gctatagttg ccagctgttt ctggtttgaa atcggctttg cgtaagatag attggatacg
    22861 agcgtaggca tattgaacgt aaggtccagt ttcaccctcg aaggatacca tagcttctag
    22921 gtcgaagtcg tatccatttg tacggtcggt tttgaggtca tagaatttaa tggctccaac
    22981 cccaacagca tgtgctattt ggtctttgtt ttctagttca ggatttttag cctcgatttg
    23041 gaccttggca cggctaacag cctctgcaac agtaggctct agcaagatga cattcccttt
    23101 acgagtagag agcttcttcc cttcttttgt aaccagacca aaaggaacgt gggtaatgtc
    23161 gtcactccaa tcgtagccca tctcttgcaa gacagctttg agctgtttaa agtgggcaga
    23221 ttgttcttga ccaacgacgt agatagattt agcaaattgg tattcatttt tacggtagag
    23281 ggctgcagct aagtcacgtg tgatatagag agttgcacca tcagacttct tgatgagggc
    23341 tggatgttca attccatatt tctcaagatt cacaacttgg gcaccttctg attcaagaag
    23401 tagtcctttt tcagaaagaa tgtctacaac tgcatccatc ttatcattgt agaaggcttc
    23461 tccgttatag ctgtcaaatt caaccttcaa ttcattgtaa aggcggttaa attccactaa
    23521 actttcatcg cggaaccatt gccaaagagc gagagcttcc tcatctccat tttcaagttt
    23581 acggaaccat tcgcgcgctt cttcatccaa gctagggtca ttttcagctt cagcgttgat
    23641 gcggacatag agtttaagga gttcatcgat tggatgagct tttacagctt cttcgtcgcc
    23701 ccattttttg taggcaacaa tcaacattcc aaattgttta ccccagtctc ccaaatggtt
    23761 gaccttgacc gtttgataac cgattttttg gaaaatatgt gacaagctat ctccgataac
    23821 agttgaacgc aggtggccaa tagaaaatgg tttagcgata ttcggactag acatgtcgat
    23881 aacaacattt tcttgtttac caatattttg gtcagcatag tgttcttttt cagtggtaac
    23941 agcttgcaat acttgagcag aaatggcaga tttatcaagg aaaaagttaa cgtaaggtcc
    24001 tgttgcgaca actttttcaa aggcctggct gttcattttt tcagccagtt cagtcgcaat
    24061 catttgtggt gctttacgtt cgactttggc aagagaaaaa gcagggaaag cgatgtcacc
    24121 catttctgag tttttagggg tttccagtaa ctttaaaata gcctcttggt ccaggctatc
    24181 aatgatgcta gccaattcgc tagcaatcaa ttcttttgta ttcattaaga gctccttttt
    24241 ggacttttct actattttat cacaatttta aagaaagaag aaaaaatttt tgaaatctcc
    24301 tgtttttttg gtataatatg gttataaata tagttataaa tatgcacgca agaggatttt
    24361 atgagaaaaa gagatcgtca tcagttaata aaaaaaatga ttactgagga gaaattaagt
    24421 acacaaaaag aaattcaaga tcggttggag gcgcacaatg tttgtgtgac gcagacaacc
    24481 ttgtctcgtg atttgcgcga aatcggcttg accaaggtca agaaaaatga tatggtgtat
    24541 tatgtactag taaatgagac agaaaagatt gatttggtgg aatttttgtc tcatcattta
    24601 gaaggtgttg caagagcaga gtttaccttg gtgcttcata ccaaattggg agaagcctct
    24661 gttttggcaa atattgtaga tgtaaacaag gatgaatgga ttttaggaac agttgctggt
    24721 gccaatacct tattggttat ttgtcgagat cagcacgttg ccaaactcat ggaagatcgt
    24781 ttgctagatt tgatgaaaga taagtaaggt cttgggagtt gctctcaaga cttatttttg
    24841 aaaaggagag acagaaaatg gcgatagaaa agctatcacc cggcatgcaa cagtatgtgg
    24901 atattaaaaa gcaatatcca gatgcttttt tgctctttcg gatgggtgat ttttatgaat
    24961 tattttatga ggatgcggtc aatgctgcgc agattctgga aatttcctta acgagtcgca
    25021 acaagaatgc cgacaatccg atccctatgg cgggtgttcc ctatcattct gcccaacagt
    25081 atatcgatgt cttgattgag cagggttata aggtggctat cgcagagcag atggaagatc
    25141 ctaaacaagc agttggggtt gttaaacgag aggttgttca ggtcattacg ccagggacag
    25201 tggtcgatag cagtaagccg gacagtcaga ataatttttt ggtttccata gaccgcgaag
    25261 gcaatcaatt tggcctagct tatatggatt tggtgacggg tgacttttat gtgacaggtc
    25321 ttttggattt cacgctggtt tgtggggaaa tccgtaacct caaggctcga gaagtggtgt
    25381 tgggttatga cttgtctgag gaagaagaac aaatcctcag ccgccagatg aatctggtac
    25441 tctcttatga aaaagaaagc tttgaagacc ttcatttatt ggatttgcga ttggcaacgg
    25501 tggagcaaac ggcatctagt aagctgctcc agtatgttca tcggactcag atgagggaat
    25561 tgaaccacct caaacctgtt atccgctacg aaattaagga tttcttgcag atggattatg
    25621 cgaccaaggc tagtctggat ttggttgaga atgctcgctc aggtaagaaa caaggcagtc
    25681 ttttctggct tttggatgaa accaaaacgg ctatggggat gcgtctcttg cgttcttgga
    25741 ttcatcgccc cttgattgat aaggaacgaa tcgtccaacg tcaagaagta gtgcaggtct
    25801 ttctcgacca tttctttgag cgtagtgact tgacagacag tctcaagggt gtttatgaca
    25861 ttgagcgctt ggctagtcgt gtttcttttg gcaaaaccaa tccaaaggat ctcttgcagt
    25921 tggcgactac cttgtctagt gtgccacgga ttcgtgcgat tttagaaggg atggagcaac
    25981 ctactctagc ctatctcatc gcacaactgg atgcaatccc tgagttggag agtttgatta
    26041 gcgcagcgat tgctcctgaa gctcctcatg tgattacaga tgggggaatt atccggactg
    26101 gatttgatga gactttagac aagtatcgtt gcgttctcag agaagggact agctggattg
    26161 ctgagattga ggctaaggag cgagaaaact ctggtatcag cacgctcaag attgactaca
    26221 ataaaaagga tggctactat tttcatgtga ccaattcgca actaggaaat gtgccagccc
    26281 actttttccg caaggcgacg ctgaaaaact cagaacgctt tggaaccgaa gaattagccc
    26341 gtatcgaggg agatatgctt gaggcgcgtg agaagtcagc caacctcgaa tacgaaatat
    26401 ttatgcgcat tcgtgaagag gtcggcaagt acatccagcg tttacaagct ctagcccaag
    26461 gaattgcgac ggttgatgtc ttacagagtc tggcggttgt ggctgaaacc cagcatttga
    26521 ttcgacctga gtttggtgac gattcacaaa ttgatatccg gaaagggcgc catgctgtcg
    26581 ttgaaaaggt tatgggggct cagacctata ttccaaatac gattcagatg gcagaagata
    26641 ccagtattca actgattaca gggccaaaca tgagtgggaa gtctacctat atgcgtcagt
    26701 tagccatgac ggcggttatg gcccagctgg gttcctatgt tcctgctgaa agcgcccatt
    26761 taccgatttt tgatgcgatt tttacccgta tcggagcagc agatgacttg gtttcgggtc
    26821 agtcgacctt tatggtggag atgatggagg ccaataatgc catttcgcat gcgaccaaga
    26881 actctctcat tctctttgat gaattgggac gtggaactgc aacttatgac gggatggctc
    26941 ttgctcagtc catcatcgaa tatatccatg agcacatcgg agctaagacc ctctttgcga
    27001 cccactacca tgagttgact agtctggagt ctagtttaca acacttggtc aatgtccacg
    27061 tggcaactct ggagcaggat gggcaggtta ccttccttca caagattgaa ccgggaccag
    27121 ctgataaatc ctacggtatc catgttgcca agattgctgg cttgccagca gaccttttag
    27181 caagggcgga taagattttg actcagctag agaatcaagg aacagagagt cctcctccca
    27241 tgagacaaac tagtgctgtc actgaacata tttcactctt tgatagggca gaagagcatc
    27301 ctatcctagc agaattagct aaactggatg tgtataatat gacacctatg caggttatga
    27361 atgtcttagt agagttaaaa cagaaactat aaaaccaaga ctcactagtt aatctagctg
    27421 tatcaaggag acttctttga caattctcca cttttttgct agaataacat cacacaaaca
    27481 gaatgaaaag gagctgacgc attgtcgctc ccttttgtct attttttaag gagaaagtat
    27541 gctgattcag aaaataaaaa cctacaagtg gcaggccctg gcttcgctcc tgatgacagg
    27601 cttgatggtt gctagttcac ttctgcaacc gcgttatctg caggaagtct taggcgccct
    27661 ccttactggg aaatatgaag ctatttatag tatcggggct tggttgattg gtgtggccgt
    27721 agtcggtcta gttgctggtg gactcaatgt tgtcctcgca gcctatattg cccaaggagt
    27781 ttcatccgac cttcgggagg atgccttccg taaaattcaa accttttctt atgctgatat
    27841 tgaacaattt aatgcgggaa atctagtcgt tcgaatgaca aatgatatca accagattca
    27901 gaacgttgtc atgatgacct tccaaattct tttcagactt cccctcttgt tcatcggttc
    27961 gtttatccta gcggttcaaa ccttaccttc tctgtggtgg gtgattgttc tcatggtagt
    28021 cttgattttt ggtttgactg ctgtcatgat gggaatgatg gggcctcgtt ttgccaagtt
    28081 tcaaaccctt cttgagcgca tcaatgccat tgccaaggaa aatttacgtg gcgttcgtgt
    28141 ggtcaagtcc tttgtccaag aaaaagagca atttgctaag tttacagagg tctcagacga
    28201 gcttcttggt caaaaccttt acattggtta tgccttttca gtagtggaac cctttatgat
    28261 gttggttggt tacggggcgg tcttcctctc tatttggctg gtcgcgggaa tggttcagtc
    28321 ggatccgtct gttgttggtt ccatcgcttc ttttgttaat tacctaagcc agattatctt
    28381 taccattgtt atggttggat ttttgggaaa ttctgtcagc cgtgccatga tttccatgcg
    28441 tcgtattcga gaaattcttg acgcagagcc agctatgacc ttcaaggata tcccagatga
    28501 agagttggtt ggaagtctta gctttgaaaa tgtgaccttt acctatccaa tggacaagga
    28561 accgatgctg aaagatgtga gctttactat tgaacctggt caaatggttg gtgtagttgg
    28621 agcgactggt gcaggaaagt caaccttggc tcaattgatt ccacgtctct ttgatccaca
    28681 ggacggggcc attaaaatcg gtggcaagga tattcgagaa gtgagtgaag gaaccctgcg
    28741 taaaacagtt tccatcgttc tccaacgtgc cattcttttt agtggaacga ttgcagataa
    28801 cttgagacag gggaagggga atgctactct atttgaaatg gagcgcgcag ccaatattgc
    28861 ccaggctagt gaattcattc atcgtatgga gaaaaccttt gaaagtccag ttgaagaacg
    28921 gggaaccaat ttctctggtg gacaaaaaca aaggatgtcg attgcgcgtg ggattgtcag
    28981 caatccacgt attctgattt ttgatgattc gacctcagcc ttggatgcca aatcagagcg
    29041 cttggtgcaa gaagctttga ataaggactt gaaggggacg acaaccatta ttattgctca
    29101 aaaaattagc tcggttgtcc atgcagacaa gatcttggtt ctaaatcaag gacgattgat
    29161 tggtcaaggt acgcatgcag acttggttgc caacaatgcc gtttaccgtg aaatctatga
    29221 aacacagaaa tgaaagacaa actataagaa aagtcaatag ttttatctaa actatttctt
    29281 atttcaattt gatgatttgg cgatgatttt agagcacggc aaaaggccct tgaaaaagtc
    29341 cattttttca aaggtaatcc tgtgttaatt tcagaaatta catcactttt tgttcgtcaa
    29401 atggcagctc tttttttagg atataaaaca gggttcggat aagttttttt gcaaggtgga
    29461 tgatggctac attgtaatgt tttccttgtt ctaatttagt cttaagatag gccttaaaag
    29521 caggcgaaaa gcgagggcat gctttggcag cttgtatgag tacctaccgc agatgagggg
    29581 aactccgttt gaccattctt cctgctaaat caatctgacc tgactgataa atagaagaat
    29641 ccagtccagc gaaagcttgt aattgagcag gattatcaaa ggcatgaata tttcgaatct
    29701 cagctaaaat gaccgcccct aaacgatccc caatcccagt acccgtcgtg atgaccgagt
    29761 tgaactcagc catcaagtca ttgacacatg tttccgcctt gtcaatgagc ctcttgtaat
    29821 gtttgatgtt ttcattacac gagataaaac gtctatgcgt tatcaaactc attaccaatt
    29881 aaaacaaaaa gctgtggtta gatcctttcg gaaattgtca agcgattgga ggaaatgaac
    29941 taatccacag cggcttattc caagtatacc acttgggctt tggcagtagc taactgcgct
    30001 aaatataata taaggaggag taaaatgaag acagttcaat ttttttggca ttattttaag
    30061 gtctacaagt tctcatttgt agttgtcatc ctgatgattg ttctggcgac ttttgcccaa
    30121 gccctctttc cagtcttttc tggacaagcg gtgacgcagc tagccaattt agttcaagct
    30181 tatcaaaatg gcaatccaga acttgtatgg caaagcctat caggaatcat ggtcaatctt
    30241 ggcctgctgg ttttggttct atttatctct agtgtaatat acatgtgtct catgacgcgc
    30301 gtgattgcag aatcgaccaa cgagatgcgc aaaggcctct ttggtaagct tgctcagttg
    30361 acggtttctt tctttgaccg tcgacaagat ggcgatatcc tgtctcattt taccagtgat
    30421 ttggataata tcctccaagc ctttaacgaa agcttgattc aggtcatgag caatattgtt
    30481 ttatacattg gtctgattct tgtcatgttt tcgagaaatg tgacgctggc tctcatcacc
    30541 attgccagca ccccattggc tttccttatg ctgattttca tcgtgaaaat ggcacgcaaa
    30601 tacaccaacc tccagcagaa agaggtaggg aagctcaacg cctatatgga tgagagcatc
    30661 tcaggccaaa aagccgtgat tgtgcaagga attcaagagg atatgatggc aggatttctt
    30721 gaacaaaatg agcgcgtgcg caaggcaacc tttaaaggaa gaatgttctc aggaattctt
    30781 ttccctgtca tgaatgggat gagcctgatt aatacagcca tcgtcatctt tgctggttcg
    30841 gctgtacttt tgaatgataa gtctattgaa acaagtacag ccctaggttt gattgttatg
    30901 tttgcacaat tttcacagca gtactaccag cctattatcc aagttgcagc gagttgggga
    30961 agccttcagt tggcctttac tggagctgaa cgaattcagg aaatgtttga tgcagaggag
    31021 gaaatccgac ctgaaaaggc tccaaccttc actaagttgc aagaaagtgt tgaaatcagt
    31081 catatcgatt tttcatactt gcctgataaa cctattttga aagatgtcag catttctgcc
    31141 cctaaaggcc agatgacagc agttgttggg ccgacaggtt caggaaaaac gactattatg
    31201 aacctcatca atcgctttta tgatgttgat gctggtggta tttattttga tggtaaagac
    31261 attcgtggct atgacttaga tagtcttaga agcaaggtgg gaattgtatt gcaagattcg
    31321 gtcttgttta gcggaacgat tagagacaat atccgatttg gtgtgtcaga tgctagtcag
    31381 gaaatggttg aggtagcagc aaaagcaacc cacattcacg actatatcga aagtttgcct
    31441 gataagtacg atactcttat tgatgatgac cagagcatct tttcaacagg gcagaagcaa
    31501 ttgatttcaa tcgctcgaac cctgatgaca gatccagaag ttctcattct cgatgaagca
    31561 acttcaaacg tagatacggt gacagaaagc aagattcagc atgccatgga ggtggttgta
    31621 gcaggtagaa ctagtttcgt cattgcccac cgcttgaaaa ccattctcaa tgcagatcag
    31681 attattgtcc ttaaagatgg agaagtcatt gaacgtggta accaccatga acttttgaag
    31741 ctaggtggct tttattcaga actctatcac aatcaatttg ttttcaaata agaaagaagt
    31801 tgtcctatgt gggcagcttt ttcttgtcca taaaaaatgt ttatcacagc cttaaaaaaa
    31861 acatattaga cgaaagtcat tttgagtgat atgataggac tatcgttagc attcgaaagg
    31921 agaggcatca tggctagaac ggttgtagga gttgctgcaa atctatgtcc cgtagacgca
    31981 gaaggcaaaa tcattcattc atctgtatct tgtagattcg cagagatcat tcgtcaagtc
    32041 ggtggtctcc ctttagtcat tcctgttggt gatgagtcag ttgtacgtga ttatgtggaa
    32101 atgattgaca aactcatttt gacaggaggc caaaatgttc atcctcagtt ttatggagag
    32161 aaaaagaccg tcgaaagcga tgattacaat ctggtccgtg acgaatttga attggcactc
    32221 ttgaaggaag cgcttcgtca gaataaacca attatggcaa tctgtcgcgg tgtccaactt
    32281 gtcaatgttg cctttggtgg aaccctcaat caagaaatcg aaggtcactg gcaaggacta
    32341 cctttcggga catctcactc tattgagaca gtagaaggaa gcgtggtggc taagctattt
    32401 gggaaagaaa gtcaggtcaa ctcagtccat cgtcaaagca ttaaagattt ggcacctaat
    32461 ttccgtgtaa ctgctattga ttcaagagac caaactattg aagcgattga gtctatcgac
    32521 gagcaccgca ttatcggttt acagtggcat ccagagtttc tggttaatga agaagatggc
    32581 aatttagaat tatttgagta tttattgaat gaactgtaac gattggaaca tctggtcgtt
    32641 ctttctttta ttttttcaaa atttttagaa tgtggcattt tacgcaaacg tttgaattct
    32701 gataaaaatt ggagaaattc gacaaaaaaa cttgaaaaaa acgaaggtaa gcgttatgat
    32761 agaaaagaag aaatattgga ggaagaacat gtcacatatt aaatttgatt attcaaaagt
    32821 tttagacaaa tttgttgcac cacatgaagt ggaatacatg caatcacaag taacagcagc
    32881 agatgaattg atccgtaaag gaactggtgc tggtagcgac ttcttgggat ggttggacct
    32941 tcctgaaaaa tacgatcgcg aagaatttga ccgcatcttg aaagctgctg agcaaatcaa
    33001 gtcagacagc gatgttttgg ttgtgatcgg tattggtgga tcttaccttg gtgcaaaagc
    33061 agcaatcgac ttcttgaatc accattttgc taacttgcaa acaaaagaag aacgcaaagc
    33121 tccacaaatc ctttacgcag gaaactcaat ctcatctact taccttgctg acttggtaga
    33181 gtatgttgca gacaaagact tctcagtaaa cgtaatttct aaatcaggta caacaactga
    33241 accagcgatt gctttccgtg tctttaaaga actcttggtt aagaaatacg gtcaagaaga
    33301 agctaacaaa cgtatctatg caacaactga ccgccaaaag ggtgctgtta aggttgaagc
    33361 agacgctaac ggttgggaaa catttgttgt tccagatgat atcggtggac gcttctcagt
    33421 attgacagcc gttggtttgc ttccaatcgc agcatcagga gctgacatca aagctcttat
    33481 ggaaggtgcg aatgcagctc gcaaagacta cacttcagac aaaatctctg aaaacgaagc
    33541 ttaccaatac gcagccgttc gtaacatcct ttatcgtaaa ggctatgcaa ctgagatctt
    33601 ggtaaactat gagccatcac ttcaatactt ctcagaatgg tggaaacaat tggctggtga
    33661 atcagaagga aaagaccaaa aaggtatcta cccaacttca gccaacttct caactgactt
    33721 gcactcactt ggtcaattta tccaagaagg aactcgtatc atgtttgaaa cagttgtccg
    33781 tgttgacaaa cctcgtaaaa acgtgcttat tcctactttg gaagaagacc ttgacggact
    33841 tggttacctt caaggaaaag acgttgactt tgtaaacaaa aaagcaactg acggtgttct
    33901 tcttgcccac acagatggtg atgtaccaaa catgtatgtg actcttccag agcaagacgc
    33961 tttcactctt ggttacacta tatacttctt cgaattggca attgcccttt caggttactt
    34021 gaatgctatc aacccatttg accaaccagg tgttgaagct tataaacgta acatgtttgc
    34081 ccttcttgga aaaccaggat ttgaagaatt gagcaaagaa cttaacgcac gtctataata
    34141 gaagaaaaga gtggtttgcc cactcttttt actctcttta tccatagaaa ttggactcag
    34201 ccaagacttg tgatataata tagaaagcaa aaaggcagac gcctagataa taggagaaac
    34261 tatgtcaaaa gatatccgcg tacgttacgc accaagtcca acaggactac tacacatcgg
    34321 aaatgctcgt acagcattgt ttaattactt gtatgcgcgc catcatggtg gaacatttct
    34381 catccgtatc gaagatactg accgtaaacg ccatgtcgag gatggtgaac gttcacaact
    34441 tgaaaacctt cgctggttag gcatggattg ggatgaaagt ccagaatcac atgagaatta
    34501 tcgccagtct gagcgtttag acttgtatca aaaatacatt gaccaactat tagctgaagg
    34561 aaaagcctat aaatcttacg ttacagaaga agagttggca gctgaacgcg aacgccaaga
    34621 agtagctggc gaaacaccac gctacatcaa tgaatacctt ggtatgagtg aagaagaaaa
    34681 agcagcttac atcgcagaac gtgaagcagc agggatcatc ccaactgttc gtttggctgt
    34741 caatgagtca ggtatctaca agtggcatga tatggtcaaa ggcgatatcg aatttgaagg
    34801 tggcaatatc ggtggtgact gggttatcca aaagaaagac ggttacccaa cttacaactt
    34861 tgccgttgtt atcgatgacc acgatatgca aatctctcat gttatccgtg gagatgacca
    34921 tattgctaat acaccaaaac agcttatggt ctatgaagct cttggttggg aagctccaga
    34981 gttcggtcac atgaccttga ttatcaactc tgagactggt aagaagttat ctaaacgtga
    35041 tactaataca cttcaattta tcgaagatta ccgaaagaaa ggttaccttc cagaagcagt
    35101 ctttaacttt attgctcttc ttggttggaa cccaggtggt gaagatgaga ttttctctcg
    35161 tgaagaactc attaaacttt ttgatgaaaa ccgcctcagc aagtcaccag cagcctttga
    35221 tcagaaaaaa ctagactgga tgagcaatga ttatatcaag aatgcagacc ttgaaaccat
    35281 ctttgaaatg gcaaaaccat tcttagagga agcaggccgt ttgactgaca aggctgaaaa
    35341 attagttgag ctctataaac cacaaatgaa atcagtagat gagattatcc cattgacaga
    35401 tcttttcttc tcagatttcc cagaattgac agaagcagag cgcgaagtca tgacgggtga
    35461 aacagttcca acagttcttg aagcattcaa agcaaaactt gaagcgatga cagatgatga
    35521 atttgtgaca gaaaatatct tcccacaaat taaagcagtc caaaaagaaa caggtattaa
    35581 agggaaaaat cttttcatgc ctattcgtat cgcagtttca ggcgaaatgc atgggccaga
    35641 attaccagat acaattttct tgcttggacg tgaaaaatca attcagcata tcgaaaacat
    35701 gctaaaagaa atctctaaat aaagaggata caataatgga catctgggaa aagatgtacg
    35761 aagaagcaca gaaactatac aatccacatg aagtatcaga ctttgtttat gccaatcatg
    35821 ttgtagctgc agtagaagca ggagatggac aaatatttac aggattctgt atggagggaa
    35881 cctgtggtgt tttccatctc tgcgcagaac gggcggccct cttcaatatg taccaatttt
    35941 caggacaaac taaggttaag aaagtattag cctttcgaga caaaccacct tatggtggaa
    36001 gttcagccat gccttgcggt gcttgtagag aattcctttt agagtttaat gctgaaaata
    36061 aagatgcaga attcatgatg gactataata taagaaaaac agttaaagtc gcagaactaa
    36121 tcccttattg gtggggagaa gaacgtgctt ctaagtttaa tgagcgatag aagagtcagt
    36181 ataattctgg ctttttttac tataagttga aaaaata
//
LOCUS       DAVKWS010000016        35744 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKWS010000016 DAVKWS010000000
VERSION     DAVKWS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.016"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(168..1802)
                     /locus_tag="VW852_000829"
     CDS             complement(168..1802)
                     /locus_tag="VW852_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272001.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYRSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1783..2532)
                     /locus_tag="VW852_000830"
     CDS             complement(1783..2532)
                     /locus_tag="VW852_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272002.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCNRIAILKKGHLIYCGSVEN
                     LRKDHPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2711..4165
                     /locus_tag="VW852_000831"
     CDS             2711..4165
                     /locus_tag="VW852_000831"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV0272003.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFIDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4255..6873)
                     /gene="alaS"
                     /locus_tag="VW852_000832"
     CDS             complement(4255..6873)
                     /gene="alaS"
                     /locus_tag="VW852_000832"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV0272004.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GNIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQLSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGR
                     NQAKIQELLDAVAGKL"
     gene            complement(6895..7380)
                     /locus_tag="VW852_000833"
     CDS             complement(6895..7380)
                     /locus_tag="VW852_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV0272005.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7655..7894)
                     /locus_tag="VW852_000834"
     CDS             complement(7655..7894)
                     /locus_tag="VW852_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272006.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8163..9233)
                     /locus_tag="VW852_000835"
     CDS             complement(8163..9233)
                     /locus_tag="VW852_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0272007.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9230..10003)
                     /locus_tag="VW852_000836"
     CDS             complement(9230..10003)
                     /locus_tag="VW852_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272008.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10000..10806)
                     /locus_tag="VW852_000837"
     CDS             complement(10000..10806)
                     /locus_tag="VW852_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272009.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10787..11944)
                     /locus_tag="VW852_000838"
     CDS             complement(10787..11944)
                     /locus_tag="VW852_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272010.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12002..12952)
                     /gene="murB"
                     /locus_tag="VW852_000839"
     CDS             complement(12002..12952)
                     /gene="murB"
                     /locus_tag="VW852_000839"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV0272011.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13064..13591)
                     /locus_tag="VW852_000840"
     CDS             complement(13064..13591)
                     /locus_tag="VW852_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV0272012.1"
                     /translation="MIYGLAMAAFFEETARLVFFKWLEKKRSLEKADALAYGLGHGGL
                     ELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQSLSVWQIYLLGFERILAL
                     GFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLSQVGWLTNPVLVEVILALEL
                     VLVAYGTKEIFCKKS"
     gene            complement(13626..13817)
                     /locus_tag="VW852_000841"
     CDS             complement(13626..13817)
                     /locus_tag="VW852_000841"
                     /inference="COORDINATES: protein motif:HMM:NF021592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV0272013.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKRR"
     gene            complement(13827..14558)
                     /gene="budA"
                     /locus_tag="VW852_000842"
     CDS             complement(13827..14558)
                     /gene="budA"
                     /locus_tag="VW852_000842"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV0272014.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14700..15971
                     /locus_tag="VW852_000843"
     CDS             14700..15971
                     /locus_tag="VW852_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV0272015.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15991..16806)
                     /locus_tag="VW852_000844"
     CDS             complement(15991..16806)
                     /locus_tag="VW852_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0272016.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16963..17616)
                     /gene="phoU"
                     /locus_tag="VW852_000845"
     CDS             complement(16963..17616)
                     /gene="phoU"
                     /locus_tag="VW852_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0272017.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17628..18386)
                     /gene="pstB"
                     /locus_tag="VW852_000846"
     CDS             complement(17628..18386)
                     /gene="pstB"
                     /locus_tag="VW852_000846"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0272018.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18399..19202)
                     /gene="pstB"
                     /locus_tag="VW852_000847"
     CDS             complement(18399..19202)
                     /gene="pstB"
                     /locus_tag="VW852_000847"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0272019.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19213..20097)
                     /gene="pstA"
                     /locus_tag="VW852_000848"
     CDS             complement(19213..20097)
                     /gene="pstA"
                     /locus_tag="VW852_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0272020.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20087..21004)
                     /gene="pstC"
                     /locus_tag="VW852_000849"
     CDS             complement(20087..21004)
                     /gene="pstC"
                     /locus_tag="VW852_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0272021.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21004..21882)
                     /gene="pstS"
                     /locus_tag="VW852_000850"
     CDS             complement(21004..21882)
                     /gene="pstS"
                     /locus_tag="VW852_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV0272022.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22456..23760)
                     /locus_tag="VW852_000851"
     CDS             complement(22456..23760)
                     /locus_tag="VW852_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV0272023.1"
                     /translation="MQFPEGFVEKYKEILGDEARNFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23761..24534)
                     /locus_tag="VW852_000852"
     CDS             complement(23761..24534)
                     /locus_tag="VW852_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV0272024.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24524..24802)
                     /locus_tag="VW852_000853"
     CDS             complement(24524..24802)
                     /locus_tag="VW852_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV0272025.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24802..25203)
                     /locus_tag="VW852_000854"
     CDS             complement(24802..25203)
                     /locus_tag="VW852_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV0272026.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25413..26030)
                     /locus_tag="VW852_000855"
     CDS             complement(25413..26030)
                     /locus_tag="VW852_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV0272027.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(26020..26793)
                     /locus_tag="VW852_000856"
     CDS             complement(26020..26793)
                     /locus_tag="VW852_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV0272028.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLIMLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIWAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26804..27541)
                     /locus_tag="VW852_000857"
     CDS             complement(26804..27541)
                     /locus_tag="VW852_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV0272029.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRCFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEELISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKEA"
     gene            complement(27546..28676)
                     /locus_tag="VW852_000858"
     CDS             complement(27546..28676)
                     /locus_tag="VW852_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV0272030.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIELDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28760..29149)
                     /locus_tag="VW852_000859"
     CDS             complement(28760..29149)
                     /locus_tag="VW852_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV0272031.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29165..29548)
                     /locus_tag="VW852_000860"
     CDS             complement(29165..29548)
                     /locus_tag="VW852_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV0272032.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29549..30337)
                     /locus_tag="VW852_000861"
     CDS             complement(29549..30337)
                     /locus_tag="VW852_000861"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV0272033.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30330..31265)
                     /locus_tag="VW852_000862"
     CDS             complement(30330..31265)
                     /locus_tag="VW852_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV0272034.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31395..31571)
                     /locus_tag="VW852_000863"
     CDS             complement(31395..31571)
                     /locus_tag="VW852_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV0272035.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31711..32418)
                     /locus_tag="VW852_000864"
     CDS             complement(31711..32418)
                     /locus_tag="VW852_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV0272036.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32569..33597
                     /gene="queA"
                     /locus_tag="VW852_000865"
     CDS             32569..33597
                     /gene="queA"
                     /locus_tag="VW852_000865"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV0272037.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34258..34647)
                     /locus_tag="VW852_000866"
     CDS             complement(34258..34647)
                     /locus_tag="VW852_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0272038.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNIDGSMATGWVQVNDSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34701..35393)
                     /locus_tag="VW852_000867"
     CDS             complement(34701..35393)
                     /locus_tag="VW852_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0272039.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT        10678 a         8148 c         6757 g        10161 t
ORIGIN      
        1 ccaaataaat aaactataaa caacaaaaat agaactatgt tatatttctt attcaaaaca
       61 ttcctcccta tatttttgat taccaatctt aatcatttac aactacattc taacaaacta
      121 taaaagcgtt tgtcgaattg aatttatcaa gcaagcgacc aaccagctca tcttttttct
      181 atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcagg
      241 aggataagag ctcctactcc caagctgatg gcaaggatag tggagagaga ctgaaccaag
      301 aatatgctcc caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg
      361 gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga
      421 tttttaacgt ccttaaagta atggtggcaa gaaaggatga cactggcaag gatccatgcc
      481 acaataaggt aaatcatcga aatgatgggc aagcctagat atagagaaag accaagcaaa
      541 gtcagaactg gtaaaaagga ctggacagca tatataatcc aaaatttcac tttcacataa
      601 cgagcaaagt caaagggtaa actcttaaga aaatcaacat tttccctctc caaggacaag
      661 gcaattgaat gcaggctggt gatattgtta ttgacaactg ctataaagag agctataaaa
      721 aacaagggta accagtatgg aggatgaatg tctggaacta tctgagaatc tcggattttg
      781 gaaatcagac cgatcatcat gagataagga aagaaagcac ttgtaaaaag cactgtaacc
      841 acgccagtcc cctgtcccaa gagggtgagg tggtagcgta aaaccatgcg aaaaaatccc
      901 tttttagtgg ttgaaattct ctccttgctg cgacgttctt ttttgacctt atcctcacta
      961 ttaagcagga tcacgtcata aaaacgagga aggaccttct ttttggtcag ataaagcagg
     1021 aagagagtta gtcctatcca agcgagcaga cccactaagg cttctgtcga aaaaggctcc
     1081 actgctattt tgtaaaagat atgaagagga taaaggagaa atggaatgtc tctaactttg
     1141 tcaacaatac ttccaaaagt cgactgaaga aagaagataa atattaaagg tatgagaact
     1201 cctatcccaa tcatcacatt cgaaaaaata gaccgatact ttctgaagac cctagtctga
     1261 gccaagaaat gtactgccac taccatcact aaagtaacag agacaaataa taaggtcaag
     1321 gacagtagca tcaaaggcaa acccaaccac agagaaggtg ctagcctaat gtagaggacc
     1381 agaaaataag ctaggattgg tacaattcca gctagagctg gcaagaggac agacagacct
     1441 ttggccatta taatctctga ttctttaaag gcatagggcc tataggaggc caggtcctta
     1501 ctctcataaa agacattgta aaatgcagtg aaagaagttg aaaaggccag caccagtaaa
     1561 atagcaacca tcgtactaaa aaaacttggc atttcctcaa aaggaaaacg aaaggctata
     1621 tttataaaca tgaaaagcat caagagacta gaaaaaatgt aagaacttag gactctagcg
     1681 gaaacattta cttttttccc aggattctta gcctgcttct ttcgtagatt agccagatta
     1741 gcttcttgag atgaatagag gatattgata tcaactaatt ttttaatgac cttgagacgc
     1801 atccgcaacc tcctcttttc taccagcaag actaaggtag atactttcca aagactggtc
     1861 tgggtggtct ttcctcaagt tctctacact accacaatag atcaaatgcc cctttttcaa
     1921 aatggcaatc cgattacaga cttgctcggc tacctctagg acatgagttg aaaacaagac
     1981 cgtcttccct ttttgtgcat gttccttcat catctgtttc aaatcaaagg cagcctgggg
     2041 atccaaacca gtcaagggtt cgtccaagac ccaaatatca ggatcagaca agagtgcccc
     2101 gatgacaaaa actttctgac gcattccatg agaaagggtt tcaataacct gataacgatt
     2161 ttcagcaaaa tcaaaaacgt tcaatagcct agctagacta gtctccaagt cagagctagt
     2221 cagatcatag gatgaggcga tcaattccca aaattcattg gccgttaaac gtaaaaataa
     2281 gtcaggcgag tctgctacgt agccaatctt tcgtttgatc gccaagcgat tttccgataa
     2341 ctcctgacca tctaccaaaa tacgaccact gctgggtgaa atgatactga ctagggattt
     2401 tatggtggtc gattttccag ccccattatg accaatcaag cccataatct ctccattttc
     2461 aatctgcaaa ttgagattac tcaaggcctc tttatcacca tacaacttac taacattttg
     2521 aaattcaatc atgggatgac tcctatcttt atctgtatta tatatcgcta tactagcact
     2581 ttgatacaaa aaaatcaata ctttatttta agtagttaaa atagtttcct tctatcttac
     2641 gcaaacgttt gctccgagag atactttatg atagaataaa gaacaagatt gacaagtaag
     2701 aggaaacatc atgcaaaatc aaacactcat gcaatacttt gaatggtatc tgccccacga
     2761 cggtcaacac tggacgcgtc tggctgaaaa tgctccacac ctagctcatc tggggatcag
     2821 tcacgtctgg atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt
     2881 ctatgactta tttgacttag gagagttcaa ccaaaaaggg actgtccgca ccaagtatgg
     2941 tttcaaagaa gactatcttc aagccattca agcccttaaa gcgcagggaa ttcaacctat
     3001 ggccgatgta gttctcaacc acaaggctgc tgctgatcac agggaagcct ttcaggttat
     3061 tgaagtggat cctgtagacc gtacagttga acttggagaa cccttcacca tcaatggctg
     3121 gactagtttt accttcgatg gtcgccaaga tacctacaat gacttccact ggcactggta
     3181 ccacttcaca ggtacagact atgatgccaa acgccgtaaa tccgggattt acctaatcca
     3241 aggagacaac aagggctggg ctaacgagga attggtcgat aacgaaaacg gaaactacga
     3301 ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga
     3361 ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa
     3421 gcatattgac tctttcttta tgcgcaattt catccgcgat atgaaggaaa aatacggtga
     3481 cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct
     3541 cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga
     3601 agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga
     3661 actcaagcct gacaaggctg tgacttttat cgacaaccac gatacccaac gaggacaagc
     3721 ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg
     3781 ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag gccagtatgc
     3841 tcaagaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag acttggccta
     3901 tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc
     3961 tgaaaatcaa tccccaatcg cagtccttat ctcaaatgac caagaaaaca gcaagtcaat
     4021 gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg
     4081 tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag
     4141 tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt
     4201 tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa
     4261 tttacctgct actgcatcca acaattcttg gatcttagct tggtttcttc ctcctgccat
     4321 ggccatgtct ggtttaccac caccacgtcc atcgatgatt ggtgctaatt ctttgacaag
     4381 gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat
     4441 agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaagt
     4501 acgaagggca ccggcatcgg atacagacaa ttgactagca atgtaacgat gaccgttgac
     4561 ttccttagca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc
     4621 agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac
     4681 ttccttgact tgaggtgctt tcaaggttgc tgcgacagct ttaagagcat cctcttgttc
     4741 acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag ttcctgaacc
     4801 gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgc caacatgagt
     4861 accaccacaa agttcaatag agtagtcacc gatagtcacg acacgaactt ccttgccgta
     4921 tttctcacca aagagggcca tagctcccat ttctttagca gtgtcaatat ccgtttcaac
     4981 tgtcttcact tcaagtgctt cccaaatttt ctcgttaact tgctgttcaa tcgcacgaag
     5041 ttcctcagca gttactgctt ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt
     5101 aagagatcct gcctgtgttg cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa
     5161 atgagtcgca gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt
     5221 atattcttgg ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg cttgaccatt
     5281 tggggctttc tgaacattgg tcacagtagc cacaacctta cctgactcat ccaagatttg
     5341 tccgtagtca gctacctgtc cacccatttc agcataaaat gacgtttccg caaagataag
     5401 agaggcagtt ccttctgaaa cagctcctac ttctgcattg tcagcaacga tagctaccaa
     5461 tttagaagac aattggctag cattgtagtt gaagacactt tctacagtga tgttttgaag
     5521 agtttcattt tgcataccca ttgagccacc cttgacagct gacgcacgcg cgcgttcttg
     5581 ctgttctttc atggctgctt caaaaccttc acggtctaca gtcataccag cttcttcagc
     5641 aatttcttca gtcaattcaa ctgggaatcc gtatgtatca tagagtttaa agacatcttg
     5701 accagcaatg acagattgac ctttttcttt caagtctgcc acaatgcctt gggcaaagtg
     5761 ttgacctgag tgaagggtac gggcaaatga ttcttcttcg ctcttaacga ttttctcaat
     5821 aaagtcacgt ttctcaagca cttctgggta gtagctttcc atgatgtttc caacagttgg
     5881 aacaagtttg taaaggaaag gctcgttgat acccaatttt tgaccatgca tagaagcacg
     5941 acggagaaga cgacgaagaa cataaccacg accttcattt cctggaaggg caccatcacc
     6001 gatagcaaat gaaagagaac gaatgtggtc tgcgataacc ttgaagctca tgttgtcgcc
     6061 atcttggtca taaaccttac cagacaattt ctcgacttca cggataatcg gcataaagag
     6121 gtccgtttca aagttggtct tagccccttg gataacagcc accaaacgct ccaaaccagc
     6181 gcccgtatca atgttcttat gtggcaattc cttgtattcg ctacgaggaa cagcagggtc
     6241 tgcgttaaat tgtgacaaaa cgatgttcca gatttcaata taacggtcgt tttcaatatc
     6301 ttctgcaagc aggcgaagac cgatattttc tgggtcaaag gcttccccac ggtcaaagaa
     6361 gatttctgta tctggtccag aaggtcccgc accgatttcc cagaagttgt cctcaattgg
     6421 aatcaagtga cttggatcca ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc
     6481 tggatagtag gtcatgtaaa gtttttcagc agggaaatca aaccattcag ggcttgtcaa
     6541 aagctcataa gcccaagtga tagcttcgtc acggaagtaa tccccgatag agaagttccc
     6601 caacatttca aacatggtat ggtgacgcgc agtcttccct acgttttcga tgtcgttggt
     6661 acggatagcc ttttgggcat tggtaatacg tggattttca gggataatgg tcccgtcaaa
     6721 gtatttctta agggttgcta ccccagagtt gatccacaaa agagttgggt catttacagg
     6781 aaccaaactt actgatggtt ctactgagtg acctttggtc gcccagaaat caagccacat
     6841 ttggcgtact tgtgcactag atagttgttt catattgtct ccttatttac ttgtttaatg
     6901 tgattggctt tccagcattt ccacatagtc aatcgcgaca cagagggaaa tgactaggtc
     6961 tgcataagcg tcttcaagaa ccgttacggt ataggtagag gtcagatgga agagttcctt
     7021 cttaatttcc gcaatcaact gatcgcgatc atccagcaat ttgaaattca aatcccagat
     7081 attgccctcg atacgaagac ctagattatc aaactcatac ttatctcgcc agaaggtcaa
     7141 cttcttacga atgacaaaac tcgagccatc ccgaagctga atctcaaaac gaggaagcaa
     7201 ggtcaagatt tctttactaa tctcactgac ttgttcacca gccgcatcat agatggtaaa
     7261 ggttttagga atcttaaaaa atgatccctc cacctgatag gcaatttctc ccctgtcatc
     7321 cttgatagcg aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa atgttttcat
     7381 caacacctcc aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc
     7441 accttcattc aatgaacttg tcattctctt gttcttatgc aattgtatga ttgagcagca
     7501 tgacttccta gcttagatgg ctcgcagcac cgccatttct ctggactaag acaagtgaaa
     7561 atcaattctc aactttctta ttataacgtt tttttaagct tgcgtcaact ggaaatgatc
     7621 tccgttgaat tagaccaatt ccctacatct ctgattactt tttcaggata tattttttct
     7681 tactgccgtt tttcttttta tcccaaattt tcatattact aaacacagct actagaatat
     7741 ttccaaatat aaaggtgcct atcacccaat atatggactc agttgttagg tattgtcgat
     7801 ccaagccatc ctttaaatgg aatagtatag cagtttggtt aacaatcata aaggttggcc
     7861 agaaactttt tttgaaaaaa gtagacattt tcattatttg ttgccgcttt ctgtaaggtt
     7921 aatactcaat aaaaatcaaa aagcaaacta ggaagctagc ctcaagctgt acttgagtac
     7981 ggcaaggcaa cgctgacgtg gtttgaagag tataggctta gtatactact aggcaagcaa
     8041 ataagcaaat aagcaaataa acaaataaac aactagaata gaaaaagata gggctctaaa
     8101 aactgacttc tattccttaa aaacgaacca gcttgactga ttcgtcttct tacgtttatc
     8161 tcctacttcc gatacatttt aaactgtagg aagaggtcgc tatatttccc tgtccattta
     8221 tggtcaaatt tctcataaac ttctaggtgt ttcatggttt caacatcggg atagaaggcc
     8281 ttatcttcct ttgtttcctc tgggagcaat tccttcgctg gtaggtttgg tgttgaatag
     8341 ccgacatact ccgcattttg gagagcattt tcaggtttca acataaagtt gataaaggca
     8401 taggctgagt tttggttttt aactgttttg ggaatgacca tattgtcaaa ccaaagattg
     8461 ctggcctctg tcggtaccac ataacgtaga ttttcatttt tttctaacat ttggctggct
     8521 tcaccagaga aggtcacgcc gattgcaaca ttattctgaa tcatatagcc cttcatctcg
     8581 tccgcaacga tagccttgat atttggagtc agtttgtaga gcttatccac tgtctcttcc
     8641 aactgctgca gatccttgga gttgagactg tagccgaggg aattgagtcc tagtcccagc
     8701 acctcacgcg ccccatcaaa gagcatgata gaattcttat actccggctt ccaaaggtca
     8761 tcccaatgct caggcgcttc atctaccatg gtttcgttgt agacaattcc taaggttccc
     8821 cagaagtaag ggatggagaa tttattacct gggtcaaagg actggttgag aaactctggt
     8881 ccgatatttt cgattccttc aatttttgaa taatcaagcg gaaccaagag gtcttcgtcc
     8941 ttcatcttgt taatcatgta ttcacttgga atggcaatat cgtaggtcgt tccaccctgc
     9001 tttatcttag tgtacatggc ttcgttggag tcaaaagtct cgtactgaac ttgaattcct
     9061 gtttcttctg taaactgagt caagagttca ggatcgatat agtctcccca gttatagata
     9121 accaattttt gactatctcg actattgatt ttactatcta aatgagtcgc aattccccac
     9181 aagacaagga taatcgctgc aattcctgct aaaaatgaat agattttttt catgcttgct
     9241 cctccttctc acgagagata aagtaataac ctacaactag gataatacta aagagaaaga
     9301 ctagagcaga cagggcattg atttctaagg aaatcccctt gcgagcacga gagtaaatct
     9361 cgactgatag ggttgaaaag ccatttcctg ttacaaagaa ggtcacggca aagtcatcta
     9421 acgaataggt gaaggccatg aaataaccag taatgataga cggagtcagg taaggaagca
     9481 tgatttcctt gaacatctga aattgactag ctcccaagtc ataggccgca tgaatcatgt
     9541 cgccattcat ttccttgagt cgaggcaaga ccatcaagac cacgatagga atggagaagg
     9601 ccacgtgact agatagaacg gtcaaaaagc caagtgaaaa cttgagttgg gtaaagagaa
     9661 tcaagaagct agcaccaatc ataacgtcag gcgcaaccat gaggatatta ttgagtgata
     9721 gaaaggcttc ttggtatttc ttacgagact ggtagatgta aatggcacca aaagtcccga
     9781 taatggtcgc tatcaaagct gataggaagg ccaagaaaaa tgtctgagcc aaaatcagca
     9841 tgagtctccc atctccaaac atggtttcaa agtgagtcca gctaaaacct gtaaagctat
     9901 tcatatcatc accagcatta aaggcatagc caatcaagta aaagataggc aggtagagga
     9961 ccagaaagac cagtcccaga taaaggttgg caaatttttt catcgttctc tcctttcctt
    10021 agtcacccac atggtgatga acatggtcag gatgagaatc acaccgatgg ttgaacccat
    10081 accatagttg tcattggtta gaaaattctg ctcaatagcc gtccccaagg tgataacgcg
    10141 gttcccacca atcaaacggg tcagcatgaa gagactcaaa cttgggataa agaccgactg
    10201 aaccccactt cgcacaccgt tcatagatag agggaagatg acatgacgga aggtctccca
    10261 cttagttgca ccaaggtcat aactagcatt gatgagatta ttatccatat cgtctaagac
    10321 attgaaaatc ggcaaaatca taaaggggag ctcgatgtag cttgcgacaa agataaagga
    10381 aaaatcggta aaaagcaact gttgtgaacc aattccgata aattccaaga attggttaat
    10441 agagccattt tgaccaaaaa tcccgataaa agcataggct ttaaggagca aattgatcca
    10501 ggtaggaagg ataatcagca tgagccagag ttgacggtgc ttgagacggg tcaaaaagag
    10561 ggctgttgga tagctgataa gcaaggtcac aaaggtcaca atgcctgcat aaagcactga
    10621 gttgaaactc attttgaggt aggtcaagtt ttgtgacgca aagtaagatt tataattttc
    10681 taaactaaac tggccttcaa tgttgaaaaa ggattgtccg aaaatcaaga ccaagggtgc
    10741 caatacaaag agggcaatcc aaagcatgta gggcactaca aagagtttag agcttgtttt
    10801 cttcatctct ttcctcctcg atcgcattga tcagacctgc ttcttgctct tcgatttcca
    10861 cgtattcttc gatacgagca tcgaactctt cttcggtttc attgagacgc atgatgtgga
    10921 tgtcttctga ttcaaagtcc agaccgattt cctcacccac gatggcctta cgggttgagt
    10981 ggatcatcca ttcatttcca agttcgtcat aggcgataat ttcataatga actccacgga
    11041 agagctgggt gtcgacctta acttggagtt tgccttcttc aggaagggta atgcgcaagt
    11101 cctctggacg aataacgacc tcaacaggtt catttggctt catcccacca tcaaccgctt
    11161 caaagcgttt gccgttaaat tcgaccaagt agtcctcaat catggtacct ggcaagatgt
    11221 ttgactcccc gataaaggtg gcaacaaagt ggttgattgg ctcatcgtag atgtccacag
    11281 gggttccaga ctggacaatc tcgccatcat tcataacgaa aatccagtca ctcatggcaa
    11341 gagcttcttc ctgatcgtga gtgacaaaga caaaggtaat gcccaatcgt tgttgtaatt
    11401 cacgcaattc gtactgcatg tctgttctca atttcaagtc cagcgctgat aaaggctcgt
    11461 ccaacaagac cacacggggt tggttgatga tagcacgggc gatggccaca cgctgacgtt
    11521 gtcctccaga aagtttgcgg atggaacgtt tttcataacc ttccaactga accatcttga
    11581 gaacttccgc tacacgctgc tcgatttctt tcttatcaat tttacgcaag cgaagtggaa
    11641 aggcaacatt ttcaaacaca ttcatatgtg ggaacaaggc ataggattgg aagacggtat
    11701 gtacgtcgcg cttgttggtt ggaatatcat tgatacgaac accgtctagc atgatatctc
    11761 ctgtcgtcgc atccagtaaa cctgcaataa tgtttaggat agttgatttc cccgaaccag
    11821 atgcacctag aagggtgtag aatttccctt cttccaactc aaagttgatg tctttgagaa
    11881 ccttggtgtt gctgtcttca aaaactttag agacgttttt gaattcgata attggttttt
    11941 tcaattggca taaattcctt cttttcatag attaaccgat cggggctctg tcaggtcccc
    12001 actacctctt gcagggagta aaaccacctg catacatctt cgctaccgat aggctttcac
    12061 ccaagatccg gacttctctt tcaagcgtaa tacctgagtg ttccttgact ttttcgataa
    12121 ccgattggat caagtcctcg tagtctttgg ccgttccatc tgcgacattg atcataaatc
    12181 ctgcatgctt ttctgacact tctacgccac cgatacgata gcctttcaag ccagcttctg
    12241 aaattaactg acctgcaaaa tgcccgactg gacgcttaaa gaccgagcca caagatgggt
    12301 attccaaagg ttgcttgagt tcacgtaggt gcgtcaagcg gtccatttcc tgcttgataa
    12361 cctgatgggt tcctggagct agggcaaatt taactgacaa gacaactgca ccagactcct
    12421 gaatagctga atggcggtaa ccaaaagcca agtctttagc agacagggtt tcgatttctc
    12481 catccttggt caagacctta caagactgca agatgtgagc aatctcgcca ccataggcac
    12541 ccgcattcat aaagacagca ccgccaacgc ttcctggaat accacaagca aactcaaagc
    12601 cagttaaact atgacggagg gcaatgcgag ttgtttcaat caagttagcc ccagcttctg
    12661 cttcaatggt atagccatca acagaaacgt tattgagctt gtcacacaag atgacaaatc
    12721 cacgaatccc accatcacga acgatgatat tgcttgcatt gccaagaacc atccaaggga
    12781 tattttcttg gttggcaaat ttcacaacgc gagccaactc aaaacgattt cgtggaaaga
    12841 ccaaataatc agcctctcca cctacttttg tataactata gctatgcaag ggttcctgaa
    12901 aacggatatc aattccttct aagatttcaa gcattttttc tcttacagac atgtcactct
    12961 tccttttaca aaattcattc cattatacca tttttagaga catttgacga ccataaaaat
    13021 accttgtttg gattttgcat aagaaaaaga ggtttccccc tttttatgat tttttacaaa
    13081 agatttcctt ggttccatag gcgaccagaa cgagctccag tgctagaatc acttcaacca
    13141 agactggatt tgtcaaccag cctacttggg aaagagatgg tgccagatca aagaaggcat
    13201 gcaagccata ggctgctagg agataaatcc atttcttctg gcgaacagct tggtaaaccc
    13261 aaactgtcaa aagtaattgg aaaccaagcg ccaagattcg ctcaaaacca agcaaataaa
    13321 tctgccagac cgaaagtgac tgaatggttt ttaacatatt ttcagacagt aattgcataa
    13381 cctgtggatt ctgagtttga actgccgaaa gaacaatgta aagattgagt aaactagtaa
    13441 ggcctagaaa aatcaactcc aagccaccat gccccagtcc ataggccaag gcgtctgcct
    13501 tttccaaact tctctttttc tccaaccatt tgaagaaaac aagacgagcc gtttcctcaa
    13561 aaaatgctgc catagctagg ccatagataa tatagacaag cgggtgatct tgcaagaggg
    13621 caatactacc gtcttttttg aggatgcaaa atcaagatat gcaccagttt ttccaaaatc
    13681 tgcgaagaga caaagaaagc gactgcaccc aagcccaaga cagctagatt aatctggtat
    13741 ttctttttgg caaaccaaat gcttcctatc agaaaagcta gcaacagcaa catggtaata
    13801 atgatatgaa tggtcatttt cttctcctat tctgcctttt caatatcttt tttcatctcg
    13861 tcaacattga acttagcaaa caagtattga cggtcttgga ctgggaaacg ttggtccaac
    13921 tggtcaactg ctcccacctc gataatgcct tccttgatga caaaatccat gacatgtcca
    13981 ccgaaggtca aatcatctga tatgaagtgc agatggtagc ctgccacact gaccccatgg
    14041 aaaatctcag gcgtccagaa accaacaata gtccccgcaa cattgtcacg actatattcc
    14101 ggttgatggg ttgcgacatc agcaaacttg gtatcgggtg ttgacttagg aatcatacgc
    14161 acatgcatat gtgaaaattc cccccgaatc ttgatagaac ggaaaagatt ttccccatca
    14221 taataagact caattcgttc tttcaattcc ttgtctgtca tctcaaagcg ctggcggaaa
    14281 atgacctctg cctgatgcgg taccactgca gcgtaaggaa taagggcatc tggtgacact
    14341 tccacaattt ctggctggtc tcctgaccct ttggcctgat aagccttgcc atccaagaca
    14401 atcaattccc catcaatgga atccaaggtt cccaaaccaa ggtcaccatg ttctagcaat
    14461 tctcctactg tcatagtacc accataaagg ccagccatca aggctccaag agtattgtat
    14521 tgaaataatt tcactggttc ctgcacgttc ttatccattc actttcttgt ctgttattct
    14581 ataaatctta ctcctaagta taccacattt gcccctagat gtgaacgaga gaaacgctct
    14641 agacattgcc aagaaggaaa aaaaagggta caatgtaaca aaatcaaggg aggtctggaa
    14701 tgaagaaaca aagcaagtac aaagaggtcg tttcctatct gaaaaatggt atcgagtctg
    14761 aacgatttcc gacgggtagt cgcctgcctt ctatccgtca actgagcctt gactttcact
    14821 gcagcaagga caccattcaa cgagccctgc tggaattacg gcacgaacaa tacctctatg
    14881 ccaagcctca gagtggctac tatgtattag aacaagggca acatcaagac ctagaaatcg
    14941 aggttaccga cgaacatgcc agtgcctatg acgatttccg actctgtgtc aatgaaacct
    15001 tgattggccg agaaaactac ctcttcaact actatgacaa tcaagaagga ttagaagacc
    15061 taagacagtc cattcacaaa ctcctctttg agcaagctct ctactgcaag gctaaccaac
    15121 tagtactgac ttctggaacc caacaagcct tgtttatcct ctctcaaata tcctttccta
    15181 gacaagccaa ggaaatcttg gtggaacagc caacctacca tcggatgaat cgcctcttga
    15241 ttgcacaggg gctggactat caaacgattg aacgaggcat tgatgggatt gacttggagg
    15301 agctggaagg ccacttcaaa acaggaaaaa ttaaattttt ctacaccatt ccccgatttc
    15361 actatcctct gggacattcc tattctgagc aggacaaacg atctattctt aacttagctg
    15421 ccaagtatga tgtctatatc gtagaggacg attatctggg tgatttggac tccaagaagg
    15481 gccaaacctt ccactatctt gatacagagg agcgtgtcat ttatatcaag tccttctcga
    15541 ccagcctttt tcctgccctt cgtattacag cactcattct tccaaatgct atcaaagaag
    15601 catttgtggc ctacaaaaat atcctagact acgacagcaa cctcattatg caaaaggccc
    15661 tgtcactcta tattgacagt caattgtttg aaaaaaatcg tttggctcgc ttgaccaatc
    15721 atgaatctta ccaaaaacaa atcgaggaaa ggataactaa aacaccttgt ccccttcctc
    15781 attatcccct acacgatggt ttattgctag acctgagaca gtatcctaaa atcgccagtc
    15841 tcaaacacag tcaactgggc ttggacttct ttgaagaggc ctatttaagc acctgtcctt
    15901 atcaatttgc caaggtgtcc ctagacaatc tggaaaatgt tttaaactat ttaaaagcag
    15961 aattggaatg acatccaact ctgctttttc ttattcttca acttcagtta gacttgctgc
    16021 tttttcaaga tagctttggt aagttccttc atccttaagt ttttggatga ccttgtctac
    16081 cacttctttc aaatcatcac tattttttct aagagcaacg gcattggcgt cgccgtcctt
    16141 catcttcaag ctgacagttg cgacagctaa gccagcgttt ttagcagcat aactaagtgc
    16201 aacaggctca tccatatgaa cagcatctac ttttccagcc tgcaattcat tgactgcttc
    16261 acccatatta gttagggaag ttaattgagc ttttggcaat tgttccttga ccattgattc
    16321 tggaacagtc cctttttggg ctgcaatatt agcactttct aggctagtta aatccttgta
    16381 tttttccaca tcagccttac gaaccaagaa actaatcttg ttttcatagt atgggattga
    16441 aaaatcaaag acttcttttc tctcgtcagt agcactaatt cctgcaactg ctaggtcagc
    16501 cttaccagtt tgaagactgg tcaaaacatt gtcaaaactc atgcttgaga tttccaactt
    16561 aaccccaagt tcatcagcga tagcctgagc catgtcgatg tctgcaccga ctacctgatt
    16621 ctttccgtca accaatgatt gaaattcaaa gggtgcatag tcaggactgg ttgccacaac
    16681 taatttcccc ttttgcttaa tggcctcaac agctgactga gaaccattag aacctgactg
    16741 gcaagctact aagaagaaac ttgcaagaaa attacataaa acaaatatcc atttttttga
    16801 tttcataaat aaacaacctc tctgttaatt ttgtataatt ataactatat ccaagttact
    16861 tgtcaagtgt tttttaaatt tttatctcaa aaatattttt tcgttcaaaa aaaggagcca
    16921 tcagttgatt tcaagctcct ttttatacag aattaaacta ttttatagtt cgacaatctt
    16981 acctgtttca aagtagacaa cccattcaca gatattttta gcatagtcac cgatacgctc
    17041 caagtaggaa ataacttgga aataatcacg acccgtaaca atggcttctg gatttttctt
    17101 aatctcttca gtcgcaaggt cacggatagt ttcaaaatag tggttaattt gctcatccat
    17161 ggaggccacc cggtatgcgt cgtcaacaga accattaaga taaagatcaa gtgctgcttc
    17221 aacaacgctt ttaacttcac gtcccatttt tttaatttct tcctctacag ctgggatgcg
    17281 ctcttccccc ttcatacgga tggttgcctg ggcaatggct acagcgtgat cccccatacg
    17341 ctccacatct gatacagcct taaggacagt caagactgta cgcaaatctt gagagactgg
    17401 ttgttggagt gcgatcattt caaatgattt cttttccagt ttcacttcgt attcatttac
    17461 ttctgcatca tcttctatga cctcttttgc caggtcacgg tcatgcgtga caaaagcacg
    17521 taccgtacga ttgatttgtg agagcacttc ttgtcccata gcgtagaact ggttatgtaa
    17581 tttctctaaa tcttcttcaa attgagatcg taacatcttt catctcctta tccaaatttt
    17641 cctgtaatat agtcttccgt ttccttgtgt tggggatcaa ggaacatctg cttggtatca
    17701 ttaaattcaa tcaaatctcc atctaggaaa aatcctgtct tatcagagat acgtgaagct
    17761 tgctgcatgg aacgggttac cagaagcatg gtgtacttgt cttttagacc atacaaggtt
    17821 tcctcaatct taccagctga aatcggatcc aaagccgaag ttggctcatc caagaggatg
    17881 attttaggac tagttgccaa gacacgggcc acgcagacac gctgctgttg accacctgac
    17941 aatccaatag ctgaatcata tagacgatcc ttgacctcat cccagataga ggcaccttgc
    18001 aaggcttttt ctacggcttc atccagaacc tgcttatcct taattccatt gatacgaagc
    18061 ccgtagacaa cattctcata gatagtcata gggaaaggat taggttgttg gaaaaccatt
    18121 ccgatttcct tacgtaattc aaccgtatct gtacgcggac tgtagatgtt gtgaccattg
    18181 tacaccacgg atccagttgt ggtcacctct ggattgagat ctcccatgcg gttgagagac
    18241 ttgaggaggg ttgacttccc tgatccagat ggaccaatca aggctgtaat ttccttaggt
    18301 tggaaagata gggaaacact attcaaagcc ttctttttat tataataaac ggacaggtct
    18361 gatacctgta aaatcgcatc tgtcatacgg tttcctttct aaccaaagtg accagataca
    18421 tagtcattgg tggactgtag cttggcattt tggaaaatag ttgcagtctt gtcatactca
    18481 atcaaatcac ccaagtaaaa gaagcctgta tagtcacttg cacgagcagc ctgctgcata
    18541 ttatgcgtta caatgatgat ggtaaagttt ttcttgagct caaacatggt ctcttctagt
    18601 tgcatggtcg caatcggatc caaggctgag gctggctcat ccattaagag gatatctggc
    18661 ttaacagaga tggcacgagc gatacagaga cgttgttgct gaccacctga taaggtcaag
    18721 gctgacttgt ggagatcgtc tttaacctga tcccagaggg cagcctgacg aagggaggtt
    18781 tctacgattt catctaggac ttgcttatcc ttaactccag cacgttcatg cgcaaaggta
    18841 atattacggt aaattgactt agcaaatgga ttggggcgtt gaaaaaccat tccaatgtgt
    18901 ttacgcattt cataaacgtt gatttctgga cggttgacat caattccacg atagagaatc
    18961 tgcccagtta ctttagcaat atcaatggta tcattcatgc gattgagact gcgtaagtag
    19021 gtagatttcc ccgatcccga cggaccaatc aaagctgtaa ttttatttct ttcaaattgc
    19081 atatcaatcc ccttaatgga ttcattttta ccataataaa catggacatc cttagtagaa
    19141 agggctactt tttcttcagg aaaggtaagg atatgcttct catcccagtt atatgttgac
    19201 atggcttctc ctttaggcag cggttaattt cttgtgtaga tagcttccga acttacgagc
    19261 tccaaagtta aaaatcagga taaagatcag gagcacagcg gcagaacctg ctgatacaat
    19321 ggttccatct ggaatagtgc cttcactatt gactttccag atatggacag ccaaggtttc
    19381 tgcttgacgg aagatagaga tggggctagt cacactgagg atattccagt tagaccagtc
    19441 aagagctggc gccgattgcc ctgctgtata gatcagagct gcagcttcgc caaagatacg
    19501 accagatgcc aagacgacac ccgttacaat acctggaagc gcttctggaa taacaacatg
    19561 aaccactgtc tcccagcgag aaatcccaag agccagacca gcctcacgtt gggtatggtg
    19621 aacgtgtttc aaactatctt ctacattacg cgtcatctga ggcaagttaa agactgtcaa
    19681 ggccaaagca cctgaaatga ttgaaaatcc atactcaaac tggactacaa agatcaagta
    19741 accaaagaga cccaccacca ctgatggtaa agaggacaag atttcaatac aagttcgcac
    19801 aaagttggta acaggacctt ttttagcata ttcagccaag taaatcccag ctcccataga
    19861 aagaggtaca gaaataatca aggtaatgac caataggaaa aaggaattgt aaagctgaat
    19921 gccaatccca ccacctgctt gaaaagcaga agaccttcca gtcaagaaag accaagagat
    19981 atggggcaag ccccgaacca agatatagag aatcaaggaa gccaagattg tcacaatgat
    20041 gctagcaatc gtatagagga cagctgttgc aagtttatct aatttcttag cgcgcataat
    20101 ttttctttcc tctttctttc gtaatcaatt taatcacact gttaaaaact aagctcatca
    20161 agagcagtac caaggccagt gaccagagaa cattattatt tacagttccc atgacagtgt
    20221 tcccaattcc catagttaat atagaagtta aagttgcagc tggtgtggtc aaggaagttg
    20281 ggataacagc tgagtttccg acaaccatct ggatagctag agcctcacca aaggcacgcg
    20341 ccatcccaaa gaccactgca gtgaaaatac cagaacgggc cgccttcaag atcacacgcc
    20401 agatagtctg ccagcgagtg gctcccatag cgaaactagc ctcacgataa taacgaggaa
    20461 ccgcacgcaa gctatccgtt gtcataaagg ttacggtcgg caaaatcatg acaaagagga
    20521 cggaaatccc tgacaaaatc ccaaaaccag tcccaccaaa gacactgcga acaaagggaa
    20581 cgacgacttg caagccaata aatccgtaca ctactgaagg aatcccaacc aggagttcaa
    20641 tagctggttg caaaatcttc gccccttttg gtgatacttc ggtcataaaa actgctgcac
    20701 caatagcaaa gggtgttgcg ataagggctg agagaatggt aacgataaag gaacccaaaa
    20761 tcataggaag ggcaccaaat tctttactag aaggattcca agttcctccc aaaagaaagt
    20821 caaagatatt cacaccattg acaaagaagg tcgacaagcc tttttgcgct acgaaaacca
    20881 aaatcatggc cacaaggatg actatcaaag aaagacaggc aaaggtcaaa ccttttccta
    20941 atttctccag acgagaattc tttgatggaa gcaacatttt cttagctaat tcttcttgat
    21001 tcattattgt ctcccttcca acactgtcac agttccggca gcatcttttt caaccttcat
    21061 ttccttaatc ggaatatact tcaatccttt gacaatccct tcttgggtct catccgagag
    21121 aacaaaattg agaaattctg cagccaactc attgggctgc cccaatgtat acatatgctc
    21181 ataagaccac aagggccaat tattgctact tatattttct ggacttaagt catagccatt
    21241 caacttcatg cttttgaccg aatcatctat ataggtaaga gataaataag agatagctcc
    21301 tggacttttt gatacgattg attttaccgc tccatttgaa tcctgctcct gactttgcat
    21361 ggcagactga ccttccataa tgacagtatc aaaggtagca cgagagccag agccggctgc
    21421 ccgattgata acagagatgg gtaagtcctt accaccaacc tctttccaat tggttacctc
    21481 acctatgaag atttgacgaa gttgctctgt cgttaggtta tcaacatcaa cctccttatt
    21541 gacaatcaga gccaagccag ctaccgcgac cttgtggtca acaagagcag aagcatcaat
    21601 tccgtctttt tcctcagcaa atacatctga gtttcctata tcaactgccc cagactgaac
    21661 ctgggacaag cctgtaccag aacctccccc ttggacattg accgtttttc caacatggat
    21721 cgtgccaaat tcatctgccg ctacttcaac caagggttgc aaggcagttg agccaacagc
    21781 cgttatggat tctccacgat caatccagct agcacagcct actaaacaag ccgtcagcca
    21841 aaaagcgata agagacagag caagcttttt tctttttttc actgtttttc tcctcgaaaa
    21901 taattatgaa tactgtgaat tttttaagta gttctttatg agttgacgca tgaattctta
    21961 ccaaatttct gcgcaattga ttatttatat aatataggct atattactct ttcctaacct
    22021 ccttttttca tatgtggata aaatctcttg tctatccctt cccccattgt cacccattat
    22081 agtcatttcg tgtctctttt tccccttttt aatgcaaggg aaattactct ccttagatga
    22141 taatccaaaa gctagaaagg tatctcaaac ctctctactc tcccagacta gtttacaact
    22201 aaaaggaaaa gattctattt tatgagaaat ctagtttaca agcggtaaga acgctaataa
    22261 ctaaacttct tgtactcttt gaaaatctct tcaaagcagt gttttgagct atctatggct
    22321 agcttcctag tttgctcttt gattttcatt gagtagtaaa actacatgta atggcaatca
    22381 agatatcaag aatcatccta ctaaaaaaat ccatactttc actataacat agaataagat
    22441 atttgactag cattttcatt tgaatctgag gccttttgga aaataatttt tcaaaacatt
    22501 tccagtaacc tttgcaaagc ccaagccatt gcctttaacc aaaacttggt accaaccatt
    22561 tggcagactt tctgccagct gaacgatttc tccagccgca tacttgacaa acgcttcttg
    22621 gccaatttca accgactgtt cgacctgact cggtttcaag gctaaaccaa gagcgaaact
    22681 gggctcaaag cgtttcttct taaaagtgcc cagatgcagt ccattgcgag caatcttgag
    22741 cttccctaaa tctggcaaaa gttctggcaa gagataaagc tggtctccaa aagtctgcaa
    22801 gatacccggt agattgacct tcaaatggtt ttgggcaaat tcctgccaca aggcaacttg
    22861 ttcacggctg aggttactct tacttgcctt aaatttagga gctggattgt tacccttaaa
    22921 ctgtagatgg gcaacaaact gaccctctcc cttaaactga tgagggtaca tccgagccgt
    22981 ttctggtagg tcaataccag ctaccattcc attgatatgc tctactggca acaagtcaaa
    23041 atcatactct tccagcaacc aattgacaat ctcttcgttt tcctcgggtg cccaggtaca
    23101 ggtcgaataa accagacgac caccttcagc taacatggtc actgcatcct ccagaatttc
    23161 tctttgcaag ctagcacatt gactcggata atctaagctc caatagtcca tagcatcagg
    23221 ttgcttacga aacattcctt caccagagca aggggcatca agaacgatta agtcaaaata
    23281 gcctttaaag accttgacca agcggtcggc agattcattg gtcaccacga catttgtcgc
    23341 tccaaaacgc tccatgtttt caaccaaaat cttagcccgt ttgcttgaaa tttcattgga
    23401 aacaagtagc ccctcccctg ctagataggc tgccagttga gttgatttgc cccccggtgc
    23461 agcagccaag tccaagacct tcataccagg actgggttgg gctacttgag ccaccatttg
    23521 agcagcaggt tcttgcgaat aaactaaacc tgtagcatgc tcaggcgatt tccctgaaac
    23581 cttcccatag tggccccaag gggtttgagt aatggcatca gaaaaggaaa gttgctcttc
    23641 ttttaaggga ttgacccgaa aggccgaaac cgcttcctcc tcaaaagagg caagaaaatt
    23701 tcttgcctca tctcctagta tttctttata tttttcaaca aatccttctg gaaattgcat
    23761 ttaagttctt ttcctttcgt aaatatagga ctgaatttcc tcctgcatct caagaggcac
    23821 catcatgaca ggctgtctgg tttgaaaatc aggagcttca ccaaaaaggg tcacaacccg
    23881 atagcccaga ctttccccta aaatactagc tgcggcataa tcccatggtt gcagataggt
    23941 gagataggtc aacaaatgcc ctgacaaaat cttggcaaaa ctaatggccg cacttccata
    24001 gacacgaaca ccaagaaccg ctcggctcaa atcagccagc ccccattcat tggtttccag
    24061 cataccacta ttccctgcaa tgagaaaatc tccaagtggt ttagttttaa aaggagctag
    24121 gggcctatca tttagacaaa ctggaaattc cccaccaccg tggtaacaat cccctttgac
    24181 cacatcataa atcagaccaa actgtccctg accattttca aaataagcca tcataacagc
    24241 aaaatcttcc tgctgggcta caaaattatt ggtaccatca atgggatcaa tgacccaaac
    24301 cttgccctct tgaaccgagg ctcgcagaca accttcttca gcacaaatct tatcctcagg
    24361 ataacgggac aaaatctcac caaccaagag ttcctgaact tctttgtcca gtctggtcac
    24421 caaatctgtt ggagaggact tggtttcaac acgcaagtct tcctgcatat ggtcaagaat
    24481 gtactgacct gctttcttaa caagctcttt agcaaattca aatttacttt ccaagagaaa
    24541 tctttccttc ccctttttct ttggcagcct gaactgctcg gtaaagtgaa tagccactaa
    24601 ccgtttcaaa ttctcgtccc aaacgtttct cttcgctctt gcttggcacg accgctttga
    24661 atcccttata ggagtccagt aactttttag cctctacttt gccttcatag gcagcttcaa
    24721 catcattaaa aaaagaaagc actgaagcaa gttcttcagt gctccacgac aaatctagtg
    24781 ggtaactata ctgtttgttc attaactaat accagctctc attcttgctt cttttagttc
    24841 ttgcttacga taactacgag ggagaaaagc acgaatctca tcttcattaa aaccgatttg
    24901 catacgcttg gcatcaataa taattggacg acgcaaaaga ctaggatact gctcaatcaa
    24961 atgaagcaat tccgataccg aaatactctc tacatcaata ttcaattttt gaaaaatttt
    25021 tgaacgagtt gaaatgatgt catcagtacc attttcggtc aaggaaagga tgtgttgcaa
    25081 ttcttttctt gttaaaggac tggtcataat attgtgttcc acaaagggaa ccttatgttt
    25141 ttctaaccag gccttagcct tacgacatga tgtacagctc ggtgatagaa atagtgtaat
    25201 catgcttttc tcttcttatc tatactttgc tacttctatt atacaaaaaa ataaagcgct
    25261 tgactagaga tttttagaaa aaaagcctat tttttcaaga aaaataggct gtttgcgaac
    25321 gattgacaca attggatttg gttaattcac tcttaacgat ggttttaaac gatatatatt
    25381 tttatatatg taaattaaaa acatctttcc tttcacttcc tacgactttt cagatacaga
    25441 tagccaaaga agttttcata gagggcaaaa aagaggagga aggcatgaag aaagaaggtc
    25501 tctggcaaaa tcataataac aggatccttg gctggatcaa aaagccaggt atcatctccc
    25561 acaaagagaa tttgatggaa aagagtaaag aattggtcaa aaccaatcaa aactccccca
    25621 agtccaatca tcacaggtaa gactactaga gccaggagac tttttcgata aagagacaaa
    25681 aagtcctttt tcacaatcct attgacaaag acatagaaac ttggcagtgt cactagagct
    25741 actagctgaa ccaaatgaaa gagattcttg accactgcga aatggtgcag accagctgct
    25801 gacgaacgaa aatcaggcat ctgtaagacc tgactaaaag gattggtcag ataattcatc
    25861 aagatatgaa aattgtattg aatggtttct ggttttagat agactcgatt cgttaagttt
    25921 agccactgaa tctccatagg atagaaaatc caagccagat aaatggtcag aaggattgag
    25981 agggagagga gaaagagcat agagccccaa aaggtcaatt tagttttcat caaaatccca
    26041 ctccgcaagg ctagaaacca catgtgtcgg tgcgattggc aggccagcta cttcttctgc
    26101 cttagtaaaa cctgtcgtca ccaagagcgt tggaatgcca ttgtcaatcc cagcccagat
    26161 atcagtcaaa taattgtccc caaccatgat taactcttca cgttccaaac ctaagtgctc
    26221 aaccgccttg tccataatga tggcattggg ttttccgata taaaccggct tcactcgtgt
    26281 cgctacttca agcagcataa tcagtgagcc agcacctggc aaaagaccgc gttccgtcgg
    26341 gatgttgagg tcaggattgg ttccgataaa atgggcaccc ttttgaatag caagagttgc
    26401 tgtggcaaat ttttcatagt cgacttgcca atccagacca actaccacgt aggcagggtt
    26461 ttccttgtct tccacataac cagccgcctt gatggcttcc ttgagtcctg cttctccgac
    26521 gacatagacg gtcttttcaa gccccaaatc attcatatag tcgatggttg ctaaagtcgc
    26581 tgtgtagaca gtcgataggg gcgtatcgat attaaaattc tgagccaaca tctccttaac
    26641 actctctgga gtgcgggttg tattgttggt tacaaagaga tagggaatgt cccgcttttg
    26701 caattcatga acaaaagtct ctccagcagg gattcggtct ttccccttat aaatggttcc
    26761 gtctaaatca attaaatagc ctttatattt catctatttc tccctaagcc tcttttattt
    26821 cttgccaagt aatgattgct tgggcattga taaccccatc acttgtaatt tcatgcttgc
    26881 tttccagtcc agtccgttca acagccgatg taatcacccc acctggtcga acttccttga
    26941 catacttgag gttgattttc ttgggaatat agtgggtcaa aaaatccgct cccatgacct
    27001 caaaaatcca gtccaagtat ttactgttat tgacatgacc attcatatcc aagtcgtaaa
    27061 aacgaacatg gtaatccttg ctgatcagct cttccaagga ctcatacttc ggtccacgga
    27121 taagtttttt atcaaaatca gactggtaag gagccacaat ctcaggttca acagcatgga
    27181 cttttcgact gtcgcggtcc atgagaacaa aggtcgccat catatggatg agctcctgcc
    27241 ccgcttcatt ataaatagta aagcaacggt agcaaaaaag tcgattgtag ctcaaggctt
    27301 ccgtttcgat ggtaatttct tccgcaaaac gaggcaaacg aaccacctca atatcatatt
    27361 ctgcgataat ccagaccaga ttatagtctt ccaaaatggc cttatcacta actcccagtt
    27421 caatcgactg catccctgaa acttgcagtg acagcaaaat cacatctgga agtttgatat
    27481 gaccgttcat atcagccata tcaaaaggaa ttttcatttt catttgataa gttaagccca
    27541 tgatcctact ccaaaataaa tcgttctgct acagtatctc ccaaaaagag acctctcttt
    27601 gtcatgcgaa cgtggtcacc ctcaatctgc atgaggcctt gttgaaccaa atctctgaca
    27661 atttctccat aaagtccagc aaaagactgt ccaaattttt cctcaaatcg cgccatggaa
    27721 accccggatt tcttgcggag tcccaagaac atttcttctt ccatttgctc cttttgactc
    27781 aggtgatctt ctgtaataca agcattgcct tcctcaaccg cactgagata atgacgaatg
    27841 ggaccatgat ttttatagcg tactccattg acataaccag atgcccctgc accaatacca
    27901 tagtattcag cattgtccca gtacatgaga ttatgacgac tttcaaagcc gggtttggag
    27961 aaattagaaa tctcataatg ctcaaaaccc gctcgctcca gctctgcaat gatgtactca
    28021 aacatctccg cttctagttc ctccttaggc agaggcaatt tcccacgtcg catccggttc
    28081 ataaagaccg tatggttttc taaaatcaaa ctatacaaac tcatgtgggg aatatccagt
    28141 tcaatggctt tagccacatt ttcctttact tgctccatgg tctgaccagg cagagcataa
    28201 atcaaatcaa tggagatgtt gtcaaaacca gccagtttca ggcgatcgat attttcataa
    28261 atatccttct ccaaatgact gcgcccaatc tttttcaaca tcttatcatc aaaggtctgg
    28321 acacctagcg aaacacgatt gacagccgaa tttttcaaaa cagctatctt atccgcatcc
    28381 aaatcgcctg gattggcttc aatggtcaac tcttccaaga cagacaagtc caagttttta
    28441 gtcaagccat tcagtaacac ctccagttgc ggagccgaca gggctgtcgg tgttccacca
    28501 ccgatataaa gggttgacaa cttttcaata tcataagaac gaaactcttc cagcagatgc
    28561 tctaaatagc tgtcgactgg ctgatttttg atgaagacct ttgaaaaatc acaataataa
    28621 caaatctggg tacaaaatgg gatgtgcaca taggctgacg ttggtttttt ctgcatagta
    28681 attattatac cacaaagact agattccaga taaaaatcac catccccaga tacatagtcc
    28741 gtccggagat ggtgatggtt tattcttctg ttatatcaat cacaatctct tctgagtcat
    28801 caagagcttc ggctttttct tgccattgct ccttgagatt atttaattga ttttttgatg
    28861 cttctgtcgc ttgaaaagca taggatttag cttgagcaag tatactgtcc acagtgattt
    28921 cacctgactc aacctgttct tttgttttca gaacaaaatc tgtagcctgc tccttaactt
    28981 ctgtcagttt ttcacagact tgctccttgg catactccgg atcttctctc aaatcatcta
    29041 gaaaatcttg agcctgactg caaacttgtt tgcccttatc acttgttaaa aacaaggcaa
    29101 gagctgcacc tgaaaccgtt cctaaaagga ttgaggataa tttacccata aggattctcc
    29161 ttttttattt tttgaaaaat ttacttgcaa gacgaagagc tgacagactt gcaccagtct
    29221 tgagtgtttt tgaaccagct gatgaagctt tcttgctcaa gacacgcgca tggtcattga
    29281 ggtctgaaac agatagagat aaatctgcaa cagcactgaa gagtggatca atcgtagcca
    29341 ccttgacatt gatatcatct gccaagacat tgaccttagc caacaactca ttggtgtgat
    29401 gcaaggtcac atccacatct gaagtcaagg ttttaatcgt cttttctgtt tcatcgatga
    29461 cacgaccaag cttttgtaca gtaatgatca gatagaccaa aaagacaatc aaagctaggg
    29521 caacaagaat atatgcaact tctaacattt agttttcctc ctctgtaata tagtaagggg
    29581 ccttctttcg attttgataa ataacgatca ttataccgag accgataagg acaactgaca
    29641 gccattggga cactcgaagt ccgaagaaca tgagactatc tgttcgcata ccttcgatga
    29701 ccatacgacc gaaaccatac caaatcaagt aaaaggccgt gatatgacct cgtctgagac
    29761 tcttccattt ccgtctaaaa atcagaatca aagcaaagcc aagcagattc cataaagact
    29821 cataaaggaa agtcggttga cggtagctcc cctcaatata catctggtca cggataaagc
    29881 caggtagata atccagatta tccactgttg caccataagc ttcttggtta aagaaattac
    29941 cccaacgccc caaactttga gcaatcataa cgctaggcgc cgcaatatct agaaaatccc
    30001 aagtattgat gagtttacgg tcagcaaaga tatagagcac aagagcccca gttatcaaac
    30061 caccgtaaat ggccaaacca ccattccaaa tggcaaaaat ctctcctaaa ttctgactat
    30121 agtaatcaaa tcggaaaata acatagtaga gacgagctcc taaaatagcc aagggaaagg
    30181 ctactaagat aaaatctaaa atatcgtctg gtatgatctt ctttctaggt gcttctttca
    30241 tggtcaaata aaccgcaaga atcaagcctg tcacaataca taaggcatac caacgaatgg
    30301 ctaggggtcc tagttgaata gcaattggat caagcatttt gcacctcatt tcgagctatg
    30361 agacttgtca gtcgttcgtc gaacaaacgg gtagcatcaa agcccatttc cttggcacga
    30421 taattcatgg cagctgcctc aatcacaaca gagatattac gacctgtttt aactggaata
    30481 cgaatacgag gaatggctac gccagaaact tcaagttcct ctgcattgtt tccaagacga
    30541 tcaaaggtct tatgcgtatc gtaattttcc aaatagacag caagctgaac ctgtgaagaa
    30601 tccttgacag cactcgcacc gtagagactc ataacatcga taataccaac cccacgaatt
    30661 tcaatcaagt gtttcaaaat ttcagctggt tcaccccaga gagtaatctc atccttggca
    30721 aagatatcga cacggtcatc ggctaccaaa cggtgaccac gtttgacaag ctcaagacct
    30781 gtctcgctct taccaattcc actatctccc tgaatcaaga cgcccatccc ataaatatcc
    30841 atcaagacac cgtgcacact ggtacgttct gccaaacgag aatccagata gctagataac
    30901 tctccagata aacgactggt agctgcacgg ctggttaaaa tagcaatctt acattctcta
    30961 gcagccttta acatctcctc tggaaccacc aaaccacggg caacaatgac tgctggtgtc
    31021 tcaggtagaa acattttttt caaaacttca taacggctgt tggaaggcat gctgatcaga
    31081 taagaccact ccttcatccc caaaagttga atccgctctg gtgtatagta gtcaaaatag
    31141 cctgtcattt caagaccagg tcgcgtaata tccgctgtat tgatttcctt ttcaagcaat
    31201 tctggttcac catagacaat atctagtcta agcttttcaa tcacttcttt tactaaaacc
    31261 gacatcattt cctccttcaa atgaattctt ctctctatta tatcaaattg taggaggaag
    31321 tttcagattt ttgcaggctt tggagtattt atttaaaaga aaaaggacta gatttctcta
    31381 gcctttcatt tcaattagaa ttttttacgt ttacgagcta cttctgattt acgtttacgt
    31441 tttacagaag gtttttcata gaattcacgt ttgcgtgttt cttgaagagt accagcttta
    31501 gtaaccgcac gtttgaaacg gcgaagtgcg tcgtcaagag attcattctt acgtactact
    31561 gttttagaca tttttttcac tcccttcaag ttcaatatct atttcattct acacgtaaaa
    31621 ggaataattg tcaagcaaaa atgcggttta attttgattt tttcttccat ttatacaaga
    31681 gttgaaagcg ttcaatagaa cattgctttt ttatttttcg agtaagctaa gcgcttcagc
    31741 atctgcgatg atggttacat cagggtgatt ttggaggcta cttgcaggta ggttctcagt
    31801 cactgggcca gatactgttc cggcaatggc ttctgctttc gactcaccat aagcaaaaag
    31861 aataatagac ttggcatcca aaatgttttt aatccccatt gaaatagctt gggttgggac
    31921 gtcttcaatc ttggcaaaga agcgagcatt ggcttcgata gtagactggt caagttctac
    31981 tagatgcgtt tgactgtcaa atggagtacc aggctcatta aagccgatat gcccattgcg
    32041 accgattccc aagatttgga ggtcaactgg atggtcagcc aaaatttggt tgtagcgttc
    32101 aacttcagct tcagcattgt ccttaacacc acgaggcaag aaactttctt taaatggttt
    32161 ttggttgaac aagttttctt gcatgaagta acgataagac tgtggattgt ccccatcaag
    32221 gcccacatac tcatcaaggt tgacactggt tagatttgaa aaatcaaggt cactctcaac
    32281 aatttccttg taaaattcaa gtgggctact tcctgtcgca agtcctagag tttgagctcc
    32341 attagccaat ttttccttca aaatctcaaa agcaactttt ccaccttgaa cttggttttc
    32401 aactttaata actttcatct tttcatcctc catttctgtc tatatttatt atatggtata
    32461 gaccaatttt tgtcaagtga aaacgattta atttctaaaa aaagagcgtc caaacttgaa
    32521 acgtgttata atggatagga ttagaactta gaaagaatga acagatatat gaatacagct
    32581 gattttgatt tccacttgcc agaggaattg attgcccaaa cgccccttga aaaacgtgat
    32641 gcctccaaac tcctcatcgt caaccgtgag acaggagaaa tgcaagataa acatttccac
    32701 tctattattg atatgctgga acctggtgat gcccttgtca tgaacgacac ccgagttctc
    32761 cctgcccgcc tctatggtca aaaagtggag acaggaggtc atgtggaact tctcctcctt
    32821 aagaacacta gtggagacga gtgggaagtt ctggctaaac ctgccaaacg cctcaaggtc
    32881 ggtactcgta tcagctttgg tgatggccgc ctcagcgctg tcgttacaga agaattgacc
    32941 cacgggggac gcattgtccg ctttgaatac caaggaattt tcctagaagt cttggaaagt
    33001 ctgggagaaa tgcctctgcc accttatatc cacgaaaaat tagatgaccg tgaacgttat
    33061 caaaccgtct acgccaagga aagcggctct gctgcagcac cgactgctgg tcttcacttc
    33121 accaaagaac tgctggcaga aatccaagct aagggtgttc atctagtcta tctgactctc
    33181 catgtcggac tcggaacctt tagacctgtt tctgtggata atctggacga acacgaaatg
    33241 cactcagagt tctatcaact ttctgaggaa gctgctgcca cccttcgctc tgtcaaagaa
    33301 aatggtggtc gcgtcatcgc tgtcggaacc acttctatcc gcaccttgga aactattggt
    33361 tccaagtttg atgggcaaat ccaagcagat tctggttgga ccaatatctt tatcaaacct
    33421 gggtatgagt ggaaggtcgt ggatgccttc tcaaccaact tccatctgcc aaaatcaact
    33481 ctggtcatgt tggtttctgc ctttgcaggc cgtgaattag tcttagatgc ctaccaccat
    33541 gccatccaag aacactaccg cttcttcagt tttggtgacg ccatgtttat ttattgagaa
    33601 agaatttctc taattcttct aataccaata aatcgctaag atattatttc aaagaacatc
    33661 tacaattgaa actctagcta gctgtagaag acgcctagta cattgaaatt aaaatgcttc
    33721 cccctagctt cgaaaatatt gccatagatt gcgttgactc tccaaattga ttcatctata
    33781 ttttatttca gcttcctata ttttcttcgc tgtttgtaaa tcaaaatgca agacacatga
    33841 gtagcaccat atttgttact cttatctgtc ctctcaagag actattatga gttatttcag
    33901 aatcattcac tactttgacc ctgactctcc ttagtctcaa aatcaaagac ttatactctt
    33961 caaaaatctc ttcaaaccgc gtcaacgtca ccttggatta tatatgtgac tgacttcgtc
    34021 agttctatct acaacctcaa agcagtactt tgagcaacct gcgactagtt ttctagtttg
    34081 ctctttgatt ttcattgagt attaaacaaa aagtgaacaa atctgaattc taatgtacag
    34141 aagactaggc ttgttcactt ttttatagtc gctataagat gaccttatct atagcttttt
    34201 atatataatt atatattcag acatactatt atcaattttg tcgcagggag gaatctgtta
    34261 acgcacccat tcaccattat cattgactct atagccatct atacttgtat tgaccgctaa
    34321 ctcacccgat gtatttacat aataccattt accaccaact tggaaccatt gattgacttt
    34381 catagaaccg ttgctgttga ggtagtacca tgaatcatta acttgtaccc aacctgttgc
    34441 catggaacca tcaatattat aaaaatacca cataccattt tcttgtttcc agcctgttgt
    34501 tggagcaact gctttagctg gttctactgc tacatctgtt ccttggttag atgtaacaga
    34561 tacaggatac gaaggaatag atgattgctc aggaacaaca actttttcag gttctctcgt
    34621 ccctctcctt atacgtcttt ttaccatctc tttagtaatt tgacgagaag tagtttcttc
    34681 aattgttcca tcacgttcat ctacagtata gattgtagta agagtaattt accaatttct
    34741 cctacttctt ctacttcttg acttttatca agagttgggc catcgagata ttctgtttcg
    34801 attggaattt cttggacaag aacttggggc ttggttcctt ttttaacaac tcttgtttga
    34861 gagtcttctt tttgacttaa agtactctca gttacttgtc cactctttcc atctacatca
    34921 taagttatcg ttgtaactgt tttcccattc tttcctagag taatctcttg ctcctgtcct
    34981 gcagaaaggt cattgtctgc ttcatattta gtagcaaatg gaacaagaac ttcttcaacc
    35041 ttgcttttag ctggaacttt gataactgta tccgtggctt cttttctatc aacagtaacc
    35101 tgttcggtaa catgaccagt ctctggatta acatcgtagg tccttgtcgt agttacatag
    35161 ccatcctctc catcaattgt aacaggattt tcactacggt cttttgtttc atctttttca
    35221 taacgaattc gcgtacttga aattttcttg gttactacct taggtttagt cgctactttt
    35281 acaataatat ccccattgtc agcgtcatca tactctattc cctcttcttt atctctagta
    35341 tcatctctga catattgaat cccatcagca gcatgaacaa aacttgtatt cagattcctc
    35401 ctaaaaataa agttagcccg gttaccgcag aaccaaaaat ctttccgagt ttacgtaatg
    35461 catagcgctt attagtatta gattttgcca ttacatccta cttctagtat agcatctttt
    35521 ctatcaaacg ttaaacaata tactttatat ataaaataga cttagaatga tatattgatt
    35581 attgaactaa cactttaact atatcgtaat caatctcata tataaaggat tgcagacatc
    35641 ttatctaaat acatgctaat atatttagat acaaacattc caacttgata attttcactc
    35701 atctttcatc attccttata caactatgca gtataaatag aata
//
LOCUS       DAVKWS010000017        35647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKWS010000017 DAVKWS010000000
VERSION     DAVKWS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.017"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(233..1381)
                     /locus_tag="VW852_000868"
     CDS             complement(233..1381)
                     /locus_tag="VW852_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0272040.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKTKFGSIQLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATELHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(1752..2159)
                     /locus_tag="VW852_000869"
     CDS             complement(1752..2159)
                     /locus_tag="VW852_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEV0272041.1"
                     /translation="MALFGKKQDESLEVELFTEEPNERVFEFKKSKTVVRIDDYFIRI
                     ARKSNVSNVLLHGLDGEKSILLSEITAYQLKEPGSTVGYLQLVYPGSSDTKGGVFDAV
                     KDENTVTFTKDEKASILELKKAIEKALKDKVKK"
     gene            complement(2204..2587)
                     /locus_tag="VW852_000870"
     CDS             complement(2204..2587)
                     /locus_tag="VW852_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV0272042.1"
                     /translation="MTEKEIISHFQVRIVDFDGELIPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPKEFDYLK
                     FMSYYNLKTVTNEVMVKEEYQTLIG"
     gene            complement(2600..2965)
                     /locus_tag="VW852_000871"
     CDS             complement(2600..2965)
                     /locus_tag="VW852_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272043.1"
                     /translation="MFLAFDRIKELADKQKISLNILEEKLGYSTNYLYSLKKGNPKSD
                     RLQEIADYFGVSTDYLLGRTENPNLADDTKEYIWQGKVLNVEEMASNVMMFGGRELTD
                     EKKKIIQSIIEGYLKEAGD"
     gene            complement(3344..3565)
                     /locus_tag="VW852_000872"
     CDS             complement(3344..3565)
                     /locus_tag="VW852_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272044.1"
                     /translation="MSKLTKEDVLQVSQEIINDAIPVIKDMLDEVFKEYPIDMEIRKA
                     ILNSVLVAHKLSTETTVSLLTELVNAQEN"
     gene            3684..3830
                     /locus_tag="VW852_000873"
     CDS             3684..3830
                     /locus_tag="VW852_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272045.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLFKQASHEIELRALES
                     ETKK"
     gene            3842..4045
                     /locus_tag="VW852_000874"
     CDS             3842..4045
                     /locus_tag="VW852_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV0272046.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVVSSVISELLKYG
                     KGSESVKEKVADVLGIENPWENS"
     gene            4062..4259
                     /locus_tag="VW852_000875"
     CDS             4062..4259
                     /locus_tag="VW852_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272047.1"
                     /translation="MNNAAQKVTRIDKDAWEIATELANEYGVSICHIISESVRYCAEN
                     AEFKEMDVVVKRLVVGSKVLE"
     gene            4270..4431
                     /locus_tag="VW852_000876"
     CDS             4270..4431
                     /locus_tag="VW852_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272048.1"
                     /translation="MNELVWFYFVVIINLIIGFATYYASKRDREKRINEFKKIQDDEL
                     ERVRNKFNL"
     gene            complement(4426..4851)
                     /locus_tag="VW852_000877"
     CDS             complement(4426..4851)
                     /locus_tag="VW852_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272049.1"
                     /translation="MEYQALIQPVISIILAIISGLWSYVASKANNKAEIEKQAKEHSH
                     TVEKLEREFHHQIDTLKQQHAFELEKIKQAHELRLQELEKVSQLDTETDKSMKMNDLT
                     YKVFTGEVDFDKALKIANKASNSKQNLNKRFIQKTSKKS"
     gene            4905..5615
                     /locus_tag="VW852_000878"
     CDS             4905..5615
                     /locus_tag="VW852_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV0272050.1"
                     /translation="MNEIFNFHGQEVRTLTIDNEPWFVGKDVADILGYSKARNAIALH
                     VDEEDALKQGIPTSGGTQDMLIINESGLYSLILSSKLLQAKEFKRWVTSEVLPAIRKQ
                     GGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAQSLLKKRKAR
                     VVACLGGIDSPAYADKVFSQSVFRQAEIDFKDHFNISRYDLLPKKHADAALAYWMTWE
                     PSTNTKMKIMKLNSFDDV"
     gene            5629..5886
                     /locus_tag="VW852_000879"
     CDS             5629..5886
                     /locus_tag="VW852_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0272051.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            5972..6292
                     /locus_tag="VW852_000880"
     CDS             5972..6292
                     /locus_tag="VW852_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272052.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPELLALPEPKQSA
                     IRFL"
     gene            6308..6601
                     /locus_tag="VW852_000881"
     CDS             6308..6601
                     /locus_tag="VW852_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272053.1"
                     /translation="MEELIEWLETKEKLNREMISDEETSDFNLHLENDNDTMFMIKEY
                     LNDYNKLAKDYRILSSENRLLKLEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            6594..7394
                     /locus_tag="VW852_000882"
     CDS             6594..7394
                     /locus_tag="VW852_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV0272054.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNRLDKDK
                     NKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENNVDLVKEALKTAINS
                     GKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            7382..7540
                     /locus_tag="VW852_000883"
     CDS             7382..7540
                     /locus_tag="VW852_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272055.1"
                     /translation="MGVLELIEQFEIDYYPLSYEKKTLLADQPIHQVVACLSEMASWH
                     ECGGRLVW"
     gene            7534..8304
                     /locus_tag="VW852_000884"
     CDS             7534..8304
                     /locus_tag="VW852_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0272056.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQARVNELADAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LVFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            8513..8731
                     /locus_tag="VW852_000885"
     CDS             8513..8731
                     /locus_tag="VW852_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272057.1"
                     /translation="MLELYFVYNGHCKFFLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGE"
     gene            8731..9009
                     /locus_tag="VW852_000886"
     CDS             8731..9009
                     /locus_tag="VW852_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272058.1"
                     /translation="MAKNILMDLAFENLHKCMGISDWKESDEVILVSSANKEQIESDE
                     SYHSAGKCNYLGKRICIFCEQVKRKSLEKMIQTLTLKNILLKRSLLSI"
     gene            9044..9232
                     /locus_tag="VW852_000887"
     CDS             9044..9232
                     /locus_tag="VW852_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEV0272059.1"
                     /translation="MSCSENLKKEKELTAAISNFKIEVLQNDDKLSSLSLSNIKRQAR
                     DLYECLVWLQYNAEESGR"
     gene            9229..9567
                     /locus_tag="VW852_000888"
     CDS             9229..9567
                     /locus_tag="VW852_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272060.1"
                     /translation="MSYDLEILVKIESGDYICIAEPKYSSPTYNLGRMFRVAMNWDFD
                     QDTTYNIADVLDNIQRGISELERYPEKYVQYEPENRWGTVSVALEVLKSLKECILEQD
                     IDTKYLYMRW"
     gene            9569..9829
                     /locus_tag="VW852_000889"
     CDS             9569..9829
                     /locus_tag="VW852_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV0272061.1"
                     /translation="MRRFIAIWILVSAGLNIWHMDRIRDLEEKKPMVVYKADNAGAEI
                     FGRVVEKGRHGKLYTVTIRDYGIFVVTKEQFEKIRVGDEVLL"
     gene            9829..10122
                     /locus_tag="VW852_000890"
                     /pseudo
     CDS             9829..10122
                     /locus_tag="VW852_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10218..10496
                     /locus_tag="VW852_000891"
     CDS             10218..10496
                     /locus_tag="VW852_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272062.1"
                     /translation="MKRLGIIIGAVFVIVVSPFVVQYGWNEIITTIVPVGKITVWQAL
                     GMDALLSFIWPVLSSKKESEEDYSYAVKSSISKIITCAFLIWLASLFI"
     gene            10508..10909
                     /locus_tag="VW852_000892"
     CDS             10508..10909
                     /locus_tag="VW852_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272063.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIINEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLYEVYLEKEYSGTFIGGMP
                     SIYLKGLLSGYAWTEDEEIIGNVYENLVYIF"
     gene            10927..11328
                     /locus_tag="VW852_000893"
     CDS             10927..11328
                     /locus_tag="VW852_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0272064.1"
                     /translation="MKKLSDEDLKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSSP
                     SVGISKPTETIAVRIADDPTLKFLEGFKAIINKLLINLVDEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            11519..12061
                     /locus_tag="VW852_000894"
     CDS             11519..12061
                     /locus_tag="VW852_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0272065.1"
                     /translation="MEFVSPIKDNDDIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            12225..12416
                     /locus_tag="VW852_000895"
     CDS             12225..12416
                     /locus_tag="VW852_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272066.1"
                     /translation="MVKEYRDDFLGEKAFEKLNKDIDANPEVGFEIVGYTQTAFVNGM
                     HIPLTAILVKWNNFFKESE"
     gene            12536..12856
                     /locus_tag="VW852_000896"
     CDS             12536..12856
                     /locus_tag="VW852_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV0272067.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            12993..13385
                     /locus_tag="VW852_000897"
     CDS             12993..13385
                     /locus_tag="VW852_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEV0272068.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            13378..15108
                     /locus_tag="VW852_000898"
     CDS             13378..15108
                     /locus_tag="VW852_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV0272069.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            15116..15334
                     /locus_tag="VW852_000899"
     CDS             15116..15334
                     /locus_tag="VW852_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272070.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNEEVVTIRQRHMKDLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            15352..16554
                     /locus_tag="VW852_000900"
     CDS             15352..16554
                     /locus_tag="VW852_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV0272071.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCEHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDDVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            16538..17113
                     /locus_tag="VW852_000901"
     CDS             16538..17113
                     /locus_tag="VW852_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV0272072.1"
                     /translation="MQKRNSYRATQFQTREEESGDLVLSGYFIKFDEETELWRGYHEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            17110..18282
                     /locus_tag="VW852_000902"
     CDS             17110..18282
                     /locus_tag="VW852_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV0272073.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVSAVEKNLEDIQKESDEKEAEKEQLEKEIEDLKNQVEELNRKAPTYPSQEKRGGQ
                     KLEQRDAIAKYIRTGQTRDIVGLKTTDSGSAALIPTEVLKPHFVNKTRNPLLDLVERV
                     KVNSGSGKYPVIKKTDGVMVSTDELKSNPELGKPAISEIDYSIKTYRGYVPVSQEMID
                     DADYDIMSIVEDEVFNQGENTELSLVTAVLKTATQADAAGFDGIKDIYNKKLKSIYKA
                     SIVVTKSMFAALDKVKDKDGRYMLQTDVASPTGYSFGGKTIYKVDDTVFGNEGDMKFF
                     IGDVTEFVKEFDRSQVSVKWVNNDIYGQLLGLFIRLDIKKADEEAGFFGTYTDVVA"
     gene            18294..18563
                     /locus_tag="VW852_000903"
     CDS             18294..18563
                     /locus_tag="VW852_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272074.1"
                     /translation="MSYKVIRPFKDLRDPKKHCYKVGDNYPRVKHKLDKEFAETLLNG
                     ANSAGSIFLMNVEDKDMSKDDTLTTEVEKEETKVVDGKPEEGDEE"
     gene            18566..18847
                     /locus_tag="VW852_000904"
     CDS             18566..18847
                     /locus_tag="VW852_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272075.1"
                     /translation="MDNVQLLELLKLKLGIATKLRDKPLEKIIEAVKTELEDNLGVLL
                     DLDSSEDQMFVVDFAAFRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            18834..19133
                     /locus_tag="VW852_000905"
     CDS             18834..19133
                     /locus_tag="VW852_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEV0272076.1"
                     /translation="MWNHEITLISKEVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEMLK"
     gene            19130..19489
                     /locus_tag="VW852_000906"
     CDS             19130..19489
                     /locus_tag="VW852_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV0272077.1"
                     /translation="MSLTSDLANEIAKAMAEYSAEVEDKIDLIAEDVVNEAVTELKAT
                     SPKRHGKYARNWRFKKNAKGSYVIYNAAPTYRLTHLLENGHVLRNGGRSRAFPHIKPV
                     EEKVKENFEKRIKEIGK"
     gene            19486..19809
                     /locus_tag="VW852_000907"
     CDS             19486..19809
                     /locus_tag="VW852_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272078.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKTGNAPDLPYLVYYESIPAINA
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWTTHELFFDVQEETFIEIERLYVKSY
                     TVYLY"
     gene            19821..20399
                     /locus_tag="VW852_000908"
     CDS             19821..20399
                     /locus_tag="VW852_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0272079.1"
                     /translation="MNQENKVTFGLKNVHVAPIKSIGADGVIAYDEIFRFPGAMELTL
                     DPRGESIPIKADDIDYHFMNSNEGYEGKFKISHIIEMFATKILGEIKDAQTGVLTEKA
                     DAEFTSFALMFEFSGDKNKTRHVLYYCSASRPGNGSKTKNGTNVNERELGFKASPRPL
                     DSVVKRSITSADNKEIYDNWFKKVYEPTAVAA"
     gene            20411..20830
                     /locus_tag="VW852_000909"
     CDS             20411..20830
                     /locus_tag="VW852_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272080.1"
                     /translation="MRKIVWVGVQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATDIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     VFPIQEVGPVLMEMLNASMTTKKHQMNQNQLAKKSSQ"
     gene            21107..23857
                     /locus_tag="VW852_000910"
     CDS             21107..23857
                     /locus_tag="VW852_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV0272081.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMVKVTSSFK
                     LQESALGSNASEAERNALAQKKIGVQSEIVSKQISNLEQQLEITKKEFGENSTQANRM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGI
                     SATETTRNAAANELYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFNSLPDPVKDFIVILGVA
                     LTIVGALAPIFLTLQAVFMSSFGAMIAAALPIIGIISGVVVAIAAIVAIVKYLWETNE
                     GFRDAVTTVWNAILEVINAVVSEISNFVMSIFGTVVAWWTENQELIRASAETVWNAIY
                     TVISTILEILGPLIQTGWDNIQLVITTAWEIIKTVVETAINVVLGIIQAVMQIINGDW
                     SGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNVLNGISGTVSNIWNGIKDTVSNV
                     LNGISGTVSSVWEGIKSTISGAINGARDAVSSAIEAIKGLFNFSISWPHIPLPHFYVS
                     GSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLNDKT
                     LGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRKTQLRGG
                     FT"
     gene            23854..24576
                     /locus_tag="VW852_000911"
     CDS             23854..24576
                     /locus_tag="VW852_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0272082.1"
                     /translation="MIKHNELVIDGVRTSSFPFKVIVHDSPSIALGESKTALLEHGGI
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKYFKGSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSAVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            24577..32577
                     /locus_tag="VW852_000912"
     CDS             24577..32577
                     /locus_tag="VW852_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV0272083.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKEIMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMALSRMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAITVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKTVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRASGGLSSAADAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELAKAK
                     AEEVKQELSDTINQRFNSFDNGPLKEAKRRAEEALRNAGASSLLAQEAKRIGLDSVAR
                     LEEFKSQTTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELAGA
                     STLLAQEAKRIELDSVARLEAFKSQTTSAQTALSGDLDALKRTIANDIRPKQAQAETE
                     IAKQVEALSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELAS
                     RIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFDV
                     TFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNVF
                     IRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLDA
                     GVSRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQKLSQVEFEVRAGSIRQ
                     EILNATKDKASKSELTQTAEELASKIASVQASGRNLFLNSLFKQDIPKTGIWTTSTYT
                     VTIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDVIISFY
                     AKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKRTTNEWLFNFNQ
                     EGTVWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRANSLEAGVSRLTEGLRT
                     KADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKAS
                     KSELTQTAEELSSKIASVQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVS
                     DSPATGFDKAIRLTSSNARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQV
                     NDNSGISPIFTLKDENWTKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDG
                     SLATSSKEAPEDIEGQISTVESTFKQRADSLAAGVNRLTEGLRTKADISALNVTAENI
                     RQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELMQTAEELASK
                     IASVQASGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGHKALKIIGLNPSGR
                     DGGNPKVTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVT
                     LSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTIWIWMPKFEISDVDTSYSEAPE
                     DIEGQISTVESTFKQRANSLEAGVSRLTEGLRTKADISSLNVTAENIRQSVKSLETDT
                     QNKLNQKLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNL
                     WIKSKTVGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIKY
                     ENVVQGRNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYSE
                     KTNFDQLKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDADGLITEAKATFE
                     RTAQGLRTDLSAIQEYVNKNGQRQEALQRYTREESARQATAVRELVNRDFVGKATYQE
                     DVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSN
                     RLTSSEQGTTTQISNISNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSA
                     NKANADSQFANVTNQLARKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQ
                     LFQVEVAKNASNGQNLLKGTKDFSGDWKNKGANWKKHAEKYKGVDVLFKNNSWNGVGQ
                     EIDAKIGEVYTFSLWMKSDWKNDTVNFYVNRNGSVEKGWGVPSETSVAITSEWKRYSF
                     TFKITVDGFIFPRVERLNQNTNLYIAGLKLEKGSYATPYTEAPEDTDEAIRSVQSQLT
                     GSWAVQNINSAGDIISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVDKLKTAN
                     FEAGSVTTTILDAEAVTAEKLKVDDALIRKLTAKDAFIDRLTSERIFSTKVESVISSS
                     TFLEAYQGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNWNY
                     AGPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIELGSKD
                     VLGDGWNPKGGRNAVVWWNQVGSGSLKYWMEQKSDRRLKENITDTAVKALDKINRLRM
                     VAFDFIENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVPYLIKAIQEL
                     NQKIEKMEKTIA"
     gene            32671..32874
                     /locus_tag="VW852_000913"
     CDS             32671..32874
                     /locus_tag="VW852_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272084.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            32877..33227
                     /locus_tag="VW852_000914"
     CDS             32877..33227
                     /locus_tag="VW852_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272085.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            33237..33653
                     /locus_tag="VW852_000915"
     CDS             33237..33653
                     /locus_tag="VW852_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV0272086.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALTLIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKAFQRLLEKDDDTKKGE"
     gene            33657..33989
                     /locus_tag="VW852_000916"
     CDS             33657..33989
                     /locus_tag="VW852_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV0272087.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            33993..34949
                     /gene="lytA"
                     /locus_tag="VW852_000917"
     CDS             33993..34949
                     /gene="lytA"
                     /locus_tag="VW852_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0272088.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPVFTVEPDGLITVK"
     gene            complement(35087..35266)
                     /locus_tag="VW852_000918"
     CDS             complement(35087..35266)
                     /locus_tag="VW852_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272089.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(35408..35557)
                     /locus_tag="VW852_000919"
     CDS             complement(35408..35557)
                     /locus_tag="VW852_000919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272090.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT        11422 a         6188 c         8082 g         9955 t
ORIGIN      
        1 aaaaaaaaat agaccttgtc cagaggtcgg ggagttagag ggggcaccct ccaaaagcat
       61 tgatttaata aggttttatt ttaccttttt cataataatc tcccttaact ccacccaatc
      121 aggtggagtt ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg
      181 atatttttcg gtatttttcg gtttttggtc ggggaattgg cggggacttt ttttagcgaa
      241 tatgactaag aaataggtct gtggtcgctt cagcaagttc gtcctctact tgattgtaac
      301 gatccgtcat ataaaccttt gtatgcccca gcgcctggct taattgttca agcggaaccc
      361 ctgcaataat gctttgagtc gtgaagaagt ggcgcatcat gtgaggtgtt acatgcaatt
      421 ctgttgcttc attcactaga ttgaagtttc tatttagctg atttggattg atgagaccac
      481 ctttctcgtt gatagttata taatccttgt gctgttcctt gataattcct aactttcgct
      541 taatcttaga agcttcagct atcagataat agatcaggtc tgttccgata tcatcaaggc
      601 agacatatcg ctctgaatcc ttcgttttaa gccctccttt cccttttaag gtctggttgc
      661 ttcgactgtc tctaagatgc agtatagccc gtccgctgtc gttctgagtg atgtccattg
      721 gacgcaatcc aaagacttct cctcttctca atccaaaaat tgtcagatag gtcagagcgt
      781 agaattgttt tggcataatc tcttctgcct ttgctatcca agtcttgaac tctttgagag
      841 tcactttctt gtttgcagca gggatatcac tctggccgat gaaaacacct ttcaagcgat
      901 ttgagagcag atttccattt ttgacggcat cattcagcaa tgccatgaag ctggaattga
      961 gggtttgaac agtgtatctg gtatggttct gcaacttttc agcgataaag agttcatact
     1021 catttctatc caaattttta agctgaatag aaccaaattt ggttttgata tgattcttat
     1081 agaggttatc attgaggtag taggaagtgt cattccagcg ccctgttgac aatctctttt
     1141 cagaataaat atcccaatat tgatcaagtg tcagattcgt attgatacct aattcttgtt
     1201 cttggatttg ttgctcaagc tctaccaagg ctgcacgagc ttgagggaga gttgtgaaac
     1261 cacttttact tttttctctt tttttacctc ggaagaaaaa agaacgtctg acatagtaac
     1321 gcttgccttt agcagtctca tagtaataga tatttgggta ttttgtttta ttatatttca
     1381 ttgtattctc cttgtttatc ggcttctgga caaggtctaa acattgagaa tattgacatc
     1441 acccctttca tggtgtaaaa tagggtatag aaaagaggcc tttttaatgg ctgatttttt
     1501 ataagggtgg gcttcacaat caaactttgg cgagggagat tgtggagctt ttttgttttt
     1561 aattcaacaa aaaacggtaa ctaaatttat agttaccgtt tctgcgtggc agcttgtgcc
     1621 aaccaggtta tttgcactag gatttcccct aggttagtaa ctatatatta tcaaatatat
     1681 ttcattttgt caaaaatgga aagttgagtc ttcacactca gaatttagag atagagagtg
     1741 tggggatttt tttatttctt gactttatct tttaaagcct tctctatggc tttctttaat
     1801 tccaaaatag atgctttttc atctttggta aaggttactg tgttttcatc ttttacggca
     1861 tcaaacacac caccttttgt atcagaagaa ccagggtaaa caagttgaag atagccaaca
     1921 gttgagccag gttctttcaa ttggtatgct gtaatctcag agaggagaat tgacttttcg
     1981 ccatcaagac catgaagaag aacattagat acatttgact ttcttgcaat cctgataaaa
     2041 taatcatcga ttcttacaac agtttttgat ttcttaaact caaaaactcg ctcattcggt
     2101 tcctctgtga agagttcaac ctctaaactt tcatcttgct ttttaccaaa caatgccata
     2161 agtagttcct ttctttttct gcttcagcag tttataaaca tatttaacca attaaagtct
     2221 gatattcctc ttttaccatg acttcatttg tcacggtttt aagattgtag taggacatga
     2281 atttgaggta atcaaactct ttggggtcgt ctaagctttc tagtgcgtct tttacgagat
     2341 gatggatcat attcctatca gcttcgtttt cacagcgtaa gcgagcgttc tggtattctg
     2401 atcgtgtgtg atccttgtgt cctagttcat gaagtaggac cttaactctc tcttttttgc
     2461 tgagtttatt agacaagaaa gctgtattgg tttctttttc gtaaaatcca agttcatcag
     2521 gtattagctc gccgtcaaaa tcgacaatgc gaacttgaaa atgacttata atttcttttt
     2581 cggtcactaa gcagtacctc taatcaccag cttctttgag ataaccttca atgatagact
     2641 ggatgatttt cttcttttca tctgttaatt ctcggccacc aaacatcatg acattagatg
     2701 ccatttcttc aacattgaga actttgccct gccatatgta ttcttttgta tcatcggcaa
     2761 gattaggatt ttcagtacgg cctagtaagt agtctgtact aacaccaaaa taatctgcta
     2821 tttcttgtaa tctatcagat tttgggttac cttttttcag actataaaga taatttgtgc
     2881 tgtatcctaa tttttcttct aaaatgttta aagaaatttt ctgtttatca gccaattctt
     2941 tgattctatc gaatgctaag aacattgttt tatcaacctt tctaagcact acgaaaaaaa
     3001 tattttaaaa ttaattataa aaactgttga caaattctaa aactagtttt aaaatagtat
     3061 tcgtaagcta aagagttagc gaactagaca actaaaaaat aaagccttac aaaactgatt
     3121 ggcgtccgtt ttctataggt aaaacttact ttagtaggtc ttttctctat gtctttattc
     3181 taaaactagt tttagaattt gtcaagtagt tcgctaactt tttagataat ttttttaaaa
     3241 ggaggtgagg aaatgagacc aagacgatat ccgtatagtg ggaaaaaaga gtccaccctc
     3301 gtaaaggcag accctgaatt agtagaaaaa attctaagaa acactagttt tcttgagcat
     3361 ttacaagttc tgttagcaac gaaaccgtag tttctgtact gagtttatga gcgacaagga
     3421 cgctattaag aatagccttt ctaatttcca tgtcgattgg gtattcttta aatacctcat
     3481 ctaacatatc tttgataact ggaatagcat cgttgataat ttcttgagaa acttgtaaaa
     3541 catcttcttt agtgagtttt gacatataaa atttcctcct ttctattgga attttgacta
     3601 aaacggtgag aggtcctagt caagattatt atagcaattt aggaagatat tacctcagtc
     3661 ttgagactga tataggaggt tgaatggaag ataaaatcat cgaacttgct gattacttca
     3721 tcagcgagaa cacaacgtac agagaagcta aaatagcgtg tgagaagcta tttaaacaag
     3781 ccagccatga gatagaactc agagcgctgg aaagtgaaac gaagaaatag aaaggagaaa
     3841 gatgagtaaa gaactaaaga taatcaaggc taaaatcaaa actcgtttga ttgagttgga
     3901 tatgactcaa gctgaattgg caaaacaagt atctgtagta tcatcagtta tttcagagtt
     3961 gctgaagtat ggcaaaggta gtgagtctgt taaggaaaaa gttgcagatg ttttgggtat
     4021 tgaaaaccct tgggaaaata gttagagcat gaggaaatag tatgaacaac gcagcgcaaa
     4081 aagtaacacg gattgacaaa gatgcctggg agattgctac ggagctggcg aacgagtacg
     4141 gcgtatctat ttgtcacatc atcagcgaga gcgtccgcta ctgtgcagag aatgccgaat
     4201 ttaaggagat ggacgttgtc gttaaacgat tggtagtcgg cagtaaggtg ctggagtagg
     4261 agggtggaga tgaatgaact agtatggttt tactttgttg tcataatcaa tcttatcatt
     4321 ggttttgcta catactacgc tagcaaaaga gatagagaaa agcgcatcaa cgagtttaaa
     4381 aaaatacaag atgatgagct tgaaagagtt agaaataaat ttaatttatg attttttaga
     4441 agtcttttga ataaatcttt tatttaagtt ttgtttgcta ttgcttgctt tattagctat
     4501 ttttaaagct ttgtcaaaat caacctcgcc tgtaaaaact ttgtaggtaa gatcattcat
     4561 cttcatagac ttgtcggttt cagtatcaag ctgagatacc ttctcaagtt cttgcaaccg
     4621 taattcatga gcttgtttga ttttttcaag ttcaaaggca tgttgctgtt ttagagtatc
     4681 tatctgatga tgaaattctc tttcgagttt ttcaacagta tgtgaatgtt ctttggcttg
     4741 tttttcaatc tcagctttat tgttagcttt tgatgcgaca tatgaccata accctgatat
     4801 tattgcaaga ataatactaa tcacaggttg aataagagct tgatattcca taagatttct
     4861 ccaatcgttt tattttaatt ataccaaatt tagaaaggaa ctttatgaac gaaattttta
     4921 attttcacgg acaggaagtc cgtactttga caattgataa cgagccttgg ttcgtcggga
     4981 aagatgtcgc agacatccta ggatatagta aggctagaaa tgcgattgct cttcatgttg
     5041 atgaagagga cgccctaaaa cagggcatcc ctactagtgg tggaacacag gatatgttga
     5101 tcatcaatga atctggtctc tactctctca ttttatccag caaattgctt caagcaaaag
     5161 agttcaagcg ttgggtgaca tcagaggtct tgccagctat tagaaaacaa ggcggattta
     5221 tccgagagga cttggatgaa gatgctttca tcgctctgtt tactggacag aagaagttgc
     5281 gtgagcaaca agcgaccatg ctggaagata ttgactacct caagagtgag caaccgattc
     5341 atccaagcta tgctcagtcg ctactgaaga agcgcaaagc tcgggtcgtg gcttgccttg
     5401 gcggtattga cagtcctgct tatgcggata aggttttttc tcaatcagtc tttagacaag
     5461 ctgagattga tttcaaagac cacttcaaca ttagtcgcta tgacttgcta cccaagaagc
     5521 atgcggatgc cgctcttgct tactggatga cgtgggagcc aagcactaat accaagatga
     5581 aaatcatgaa attgaactca tttgatgacg tctagaaagg ggaagaagat ggacaatgtt
     5641 ctactttcac tgtctgaatg gattaaatct attatcaagg acacaatcac aaggctagtc
     5701 gaaatagaaa aagatagtga tcactatcca gagttgatgg atgtgaacac tacctgcgaa
     5761 tttctaggaa ttaagtatgc caccttttca gataattatc gttacttaaa gggatttcca
     5821 aaggaattac ctggtaagaa atggtcaaaa agagccatca aagaatggct ctctaatcaa
     5881 atataataac tttactaaaa ggcttctgga caaggtctta gcaaaattat ttgactatat
     5941 tatagcacaa aaagaggata aggagataaa aatgtttgaa ccaccgattt tagaccagct
     6001 gatgggggtt ggagccttac tgcttggatt tgcaggggct tgccgtcata tcaaattgca
     6061 ggaacaacgc aaggaagaag aaagacgaga agagcaagaa tttgcgtcta tgattatcca
     6121 agtgcgtaat catgcatatg aacgtggtag agaggacaaa tggcaagaaa ttcgcaagaa
     6181 tattcgcaga gagttcaaag gattcacata tgacaacgaa ccgcctgtag gattgcgccc
     6241 tgagctgtta gctttgccag aacctaaaca gtctgcaatc agatttttgt aatgaggagg
     6301 tcaggaaatg gaagaattga ttgaatggct tgaaaccaaa gaaaaattaa acagggaaat
     6361 gatttctgac gaggaaacat cggactttaa cctacatcta gaaaatgata atgataccat
     6421 gttcatgatc aaggaatact tgaacgatta taacaagcta gccaaagact atcgaattct
     6481 atcgtctgaa aatcgtctac ttaagcttga aaaaatggag ctggaaggca ggtacatcta
     6541 tgaggatatg cggatgaagt accgcgctaa ccgtaggaag tggggtgcta ggtatgtctg
     6601 aaattaagtg gataaaaatc acaaccgata tttttgacga tgaaaagatt tgcctgattg
     6661 atgccttgcc tgatcctgat gccatcttag tgatatggtt caagattttg acacttgctg
     6721 gaaaacataa cagtaatggt ttgttgatga tgactgataa ggttcactat acagatgaaa
     6781 tgttagctac tatttttcgt agaccattga atacagtaag aatggctatt ggagtttttg
     6841 aacagtttgg gatgattgag attatcgatg gtattattag cttgccaaat tgggaaaaac
     6901 atcaaaacgt tgacggaatg gagaaaatca aggaacagac acgtaaccgt gtagccaaat
     6961 accgtaaaaa acaaaaaaat cttgctcttg gtaacgttac aggtaacgtt acagtaacgg
     7021 acggtaacgc actagaagaa gataaagata agaatagatt agataaagat aagaataaga
     7081 aaagaataac tactactagt agtggtagtg aagaaaatat cttagaactt tttcaatctg
     7141 agtttcgtag actcttatct ggatttgaaa ttgaagaaat caaccatcta ctaaatgaga
     7201 ataatgtgga tttggtgaaa gaagcattga agactgctat taactcagga aagccgaaca
     7261 tcaaatatat tggtgggatt ttaagaaatt ggcagatgaa caatgttacc actgttgaac
     7321 aggttcgtca atcggaaaag aagaacaagg ataagaaaga agaacaggag gccaaggacg
     7381 aatgggggta ctagaactaa ttgaacaatt cgagattgac tattatccgt taagctacga
     7441 gaagaaaact cttttagcag accaaccaat tcatcaagtg gttgcctgct tgtctgaaat
     7501 ggctagctgg catgaatgcg gaggtcggct agtatggtag acaatgtgtt tgaggaaatc
     7561 gccttatctt atcgtaggaa tacagaacaa caagaagagt tctgcgaaaa gcataacatc
     7621 cctttgataa agatattgag gaccgagagt gttgtatgtc gcatgtgtga atctgagcgg
     7681 attcatgagg aaaatcaagc aagagtgaat gaattggccg acgctgagaa tgagcgagag
     7741 aggaaatact atcttgagaa gttttctctt tatgatgagg ttttgaaaaa tgcgactttg
     7801 gacaattttg aaacaccaac tgaaaaagaa gcagaaaagc tagtttttgc aaagaggatt
     7861 tgtcgtgagt ggtctgaggg tgctaggaac aacatcgtgt tacaaggaga agctggaaca
     7921 ggtaagagcc atttggcctt tgcgatggtt aaggctctat ctgagtacac gaaagagatt
     7981 gctattttta ttaacgtaac tgacttgctg atgaagatta aagctgattt tagtcaggaa
     8041 gagtttctgg tcaataaaat tgctagtgct aagttcttgg ttttggatga tttaggaatg
     8101 gaaaaagata gcgaatggtc atttactatt ctctacaata tcctgaataa gcgttcaaat
     8161 acaatcatta ccacgaattt gatttctgct gatattcaga aaagatatgg cagacccttt
     8221 atgtccagac tgatgaaggg ggtggataaa gaccatttga tggttttcaa tgatttgacg
     8281 aacaagcgga aacaatattt ttagaatgga ggtggctgat gtttatttta aagcatggga
     8341 caagagagga taagccgttt ctgagatccg tggttattgg agtgactggc ttggacattt
     8401 catgttctga ggagaagaaa gcattgcggt ttgtttctcg ggcggcagcc ttacaggtta
     8461 gcaaggcatt gagaggttcc tttgggaact tctaccctgt cgaggtggag tgatgttaga
     8521 gctttatttc gtctacaacg ggcactgtaa gtttttcctt gggaggtttg acaatgtcga
     8581 tgatctcatt gagcagatgg aagaccatca gtgggctttc tcagctatca ctcatccaag
     8641 gtttcagaag cacattggtc agcggacgac acgatttgac tacggttcta aggattgtta
     8701 ctatttagcg actttttcag gaggagaata atggctaaaa atattttaat ggatctagca
     8761 tttgagaatc ttcacaaatg tatgggaatt tctgattgga aagaatctga tgaagtaatt
     8821 cttgttagtt cagctaataa agaacaaatt gagtcagatg aaagttacca ttcagccgga
     8881 aaatgtaatt atcttggcaa acgaatttgt atcttctgtg aacaagtgaa aagaaagagt
     8941 ttggaaaaaa tgatccagac tttgacattg aaaaacattt tgttgaagag gtcgctattg
     9001 tcaatttaag taaatttcat agttactttg agggggtgga gtgatgtcat gtagtgaaaa
     9061 tttaaaaaaa gaaaaagaat tgactgctgc tatttcaaat ttcaagatag aagtcttaca
     9121 aaatgatgat aaattgagca gtctatcatt aagcaacatc aaaaggcaag caagggatct
     9181 atatgaatgc ttagtatggt tgcagtataa tgcggaggaa tcaggtagat gagttatgat
     9241 ttggaaatat tagtaaaaat agagagtgga gattatattt gtatcgctga acctaaatat
     9301 agttctccga cctacaatct tggaagaatg tttagagttg ctatgaactg ggattttgat
     9361 caagacacta cgtacaacat cgctgatgtt ttagataaca ttcaacgcgg tatctctgaa
     9421 ctagaacggt accctgaaaa gtatgtgcag tatgaacctg aaaatagatg gggaacagtt
     9481 agcgttgcat tggaggtttt aaagtcactg aaagagtgta ttttagaaca agatattgat
     9541 acgaaatatt tatatatgag gtggtaatat gagacgattc atagctatct ggattcttgt
     9601 ctctgccgga ttgaacatct ggcatatgga caggattcga gatttggaag agaaaaagcc
     9661 gatggttgtc tataaagctg ataacgctgg cgctgagata ttcgggcgtg tcgttgaaaa
     9721 aggacggcat gggaagttgt atacagtaac tatcagagac tatgggattt tcgtagttac
     9781 gaaagagcag tttgagaaaa tcagagtagg ggatgaggtg ttactctaat ggatgatatt
     9841 ttacaagctt tagcaaaaat gctaaatatg actgttgatg aagtaagttc tttgcttaca
     9901 acatttaaag ggaatgcacc acagatttat gaaatgttcg ttaaagaaaa gatgttttat
     9961 gatctcttca gtctttttca aatcatgtca attgtaatat ttagtatttc tgcagtagtt
    10021 ttagcagttt taactctcat atattttaca tacgatggtg gttttgttta ttcctatgat
    10081 atacgtacag gaaaaaccga ggaagaaatt aaattagaac gcaagagaaa gtcaaacaat
    10141 ggtttattga ccgtgattta gaaaacggta gggtcgttgg attgatggtg ctttcgtcaa
    10201 agaggaggat ttataaaatg aaaagactag gaatcattat tggggcggta tttgtaatcg
    10261 ttgtatcgcc gtttgtagtt cagtatggat ggaatgagat tatcacaaca attgttccag
    10321 ttggtaaaat tacagtctgg caagcattag ggatggatgc actactatct ttcatctggc
    10381 ctgtgctatc tagcaaaaaa gaatctgaag aggattattc gtatgcggta aagagcagta
    10441 tttcaaaaat cattacatgt gcatttttga tatggttagc tagtttgttt atttaaggag
    10501 tgttagcatg ataccaaaat ttagagggtt atccattgac gaaaaaagca aaggagaatg
    10561 gcaatacgga catttaattg aagatagagg aagagcattt attatcaacg aagtggtaga
    10621 agccaatgaa caatacatta ctataggttc ttggtgtcct gtaaatccag caacattagg
    10681 acaatcaaca ggactcagag acaagaacgg caaggaggta ttcatcggtg acatcgttaa
    10741 atgtacaaga ggatgtctct atgaagtata tttagaaaaa gaatacagtg gcacattcat
    10801 aggcggaatg ccttccatat atctaaaggg attgctaagt gggtatgcgt ggactgaaga
    10861 cgaggaaatc atcggcaatg tctacgaaaa tctagtttac attttttaaa aacttggagg
    10921 tggaaattga aaaaattgag cgacgaagac ctcaaaacat tagacagaga acttttcaaa
    10981 tttcaaaaca ttcaacgtac aatagatttg agaaggctag aattagaaac tcgaaaccca
    11041 gatgctcaga gtagtcccag cgtaggaata agcaaaccta ccgaaactat cgcagtcaga
    11101 atcgcagatg atccaacctt aaaatttctc gaagggttca aagctattat taacaaactc
    11161 ttgatcaatc tagttgatga agataaggaa atctttaatc tgcgctggag atatcctcaa
    11221 ctgagatggg aagaaatagc agaacagaaa ttcatgagca aagctacaat ctatcgacgt
    11281 agaaggatta tcttagaaca gtacgctata ctgaaaggtg agttgtaaat aagattgaga
    11341 caaaagacat cttgaagtct cataaaaaaa ggtctattat gatagcatga acttctgaaa
    11401 caaaaacaca catcacactt gaggagtcat ccttaattct agtccaaaag ttgtccaaca
    11461 gaagtatcgt caagagtcag caaatgctgg ctttttgttt ttggaaagga ggtagaatat
    11521 ggaatttgta tcaccgataa aagataatga cgacattcag gcaatgaaag attatcttag
    11581 agagtggaat gagatgtatt atatgctatt cattacaggc ctgaatactg gtttgcgagt
    11641 cggagatata cttaccttga aagttaaaga tgttcaaggc tggcacatca aactgagaga
    11701 acggaagact ggcaagcaga taacaagacg gatgacaaaa gaactcaaga aagaaatgag
    11761 gagatatgtt gagggcaaac catttcatca tttcttattc aagagtaggc aaggtcagaa
    11821 taaagcaatc actcgtgagc gagcctatca aatcatacat gaagcagctg aagaacttgg
    11881 cattgataat gttggcacac atacaatgcg caagacattc ggctataaat attacaacaa
    11941 gacaaaggac gtagggacat tacagaaaat gttcaatcac tcatcacctg caattaccct
    12001 gagatacata gggatagaac aagcagagct cgatgatgct ttacggaact ttgtcattta
    12061 attttttaga tattactttc acataatgag ttaagcataa actgaaaaaa tgaaactctt
    12121 taaaacccat gcttagtaag ggtttgagat ttagagtgag tttaacaaaa tataagatat
    12181 gtgaaagtga gggataaaat tggtatagtt acaagaggtg aaaaatggta aaagaatacc
    12241 gcgatgattt tcttggagaa aaggccttcg agaaattaaa taaagatatt gatgccaatc
    12301 ctgaagttgg ctttgaaatt gttggatata ctcaaacagc atttgtaaat ggaatgcata
    12361 taccgttaac agccatacta gtaaaatgga ataatttttt taaagaatca gaatgagaca
    12421 aaagacatct tgaagtctca caaaaaaagg tttattatgg tagcatagat ttcttgtatg
    12481 aggaggggat aggtcaaagg cctgtccctt ttatcattga gaaaggaggt ttgaggtgta
    12541 taacaaacct atcagaccat ccttgaaatc taagaagtgg gagaagttcc gtgataggat
    12601 aatgcgtaag catgattatc tttgccaaga aagtttgcgt tacggaattt ctgttcaagc
    12661 agaaatggta catcatatct ttcctgtgtc tgaatatcct gagcttgaat ttgttgaatg
    12721 gaattgcttg ccattgacga ataagaaaca taatacgttt catgatagag tgaacgatag
    12781 agtaatcaac caaggattgt attggcagaa aaaaagaaaa aaagaatttt taaatttttt
    12841 caaaaatgaa aaatgaaaat ttttaacccc ccctcttttt gaaaaatcat tttggccagt
    12901 agggtaccgg tgaagggaac tttttccaag tcgggggcct tcaaacaaaa agggggtaaa
    12961 aactaagcga ttttgacgaa aggaggtagt ttttggctaa accaattaca gcaaagtcga
    13021 ttaagtcaaa agtggtcaag cagatgaaag acttgggaac ttatcgtaaa gagttcgaaa
    13081 tgatcattga catttttgca ggtatgttat accagtatca gaaacttgct caagattatg
    13141 ctgatatggg ttatccagta acagacacct acgtcaataa ggctggtgct gaaaatgagc
    13201 gcaaagttcc aatcttgaca gcgatggaaa ttttgaggaa agatatactc agctactcta
    13261 atcagttgat gatgaatcct aagtcgctcg gtgaggtagt agaacaagag ggtgagtcag
    13321 ttcttactga ggtactgaag ttcaagaacg aaatcaagaa gaagcgagtg actggcaatg
    13381 ggtaatcttg ataaagctaa aaaatacgct caacacgtct taactcaccg agaagaacat
    13441 tgcgaagaaa acatccttgc tgctgaacgt tttttccgtg atttagaaaa tccaaccttt
    13501 gagatggatg aggatatggt ggattttgtt attcacttta tcgagaacgt gatagttcat
    13561 cagcagggcg atgatatgtt tgcggtatct atccgtaaca agccattgct tttgcaaccg
    13621 tggcaacatt tcgttgtagt taatctgttt ggtttttact ataagggtac gaatgagcgc
    13681 aggttcaaag aagcgcttat catgcttgct cggaagaatg ggaagacctc gtttactgct
    13741 gcaatcgcac ttgcttatca gatattagat acagatagcg gttccaaatg ctatatcgtt
    13801 gctaactcag tcaagcaagc gatggaagct tttggttttt taagattcaa cgttgaacgc
    13861 tggaacgata agaacattcg tatcaaggat aataaccaag aacactctat caccgccaat
    13921 tttggtgatg aaggttcttt ctttatccag gctttagcga atgatgagag ccgtctggac
    13981 tctttgaacg gaaatgttat catcttggac gaagctcaca caatgaggaa cagtaagaaa
    14041 catggtctta tgaaaaaaac aatgtcagca tacagaaaca gtatgctttt tgttatctca
    14101 acggctgggg atattcctac cgggttcctt gctaaccgtc tgaaatactg tcaaaaggtg
    14161 ctcaagcaat tagtcactga tgattcattt ttcatcttca tctgcaaggc taatcaatct
    14221 gctgatgggg acgtggtgaa ctatctggac gagaatatcc tcaagatggc taatccgtca
    14281 tggggtgtca cggtttcgct caaggctctc aaggaagaag cagagcaggc tatgaatgat
    14341 cctcagacaa gaaatgagtt tttcaataag accttgaata tcttcactaa ctctatgaac
    14401 gcttatttta atcctgatga gtttattgcg tcggatagtt gctacgattg gagtttagaa
    14461 gagctggcac gtttgcctat tcgttggtat ggtggtgcgg acttgtcaag attgcacgac
    14521 ttaacagctg ctgctctcta tggtgtctat catgacggtg agaaagacgt tgatatctgt
    14581 atcacacatg ctttcttccc tcggattaat gctcagaaaa aagctaatga tgacgggatt
    14641 ccactctttg ggtggcagtc tgacggttgg ttgacgatga gcaacactcc gaccgtcctc
    14701 tatgatgata tcgtcaaatg gttcatcaag atgagggaga aagggttcaa gattgctgct
    14761 gtcggaatgg ataggaagtt tggccgtgag ttcttgacga agatgaaaca agctcggttc
    14821 aagatgattg accaacctca gcttttttat ctgaaatcag agggattcag acggattgag
    14881 tttaaagtta agaataaaga attttactat cttcattctg atgcttatga atactgtgtg
    14941 agcaacgtta gagcaattga aaaggtggat gacgctgtgc aatatgaaaa attagatgga
    15001 gacggtggga ctgcaaggat tgacttgttt gatgccagcg tctttgcttg tatacaggct
    15061 cttgctaatc ttggcaaggg tggtgatgtg atgagattct ttgattaggt gaattatgaa
    15121 tgaaatagta ttatcagaac atgaaattaa cttgctgatc aacaaagggc gagttaaagt
    15181 aattttaaac gaggaagtag taactattcg tcaaagacat atgaaagatt tgatggctga
    15241 aacagtaaaa tgggaaaaac aggtaattga tgtcagtcag aatatcgtaa gaaataaaca
    15301 ctttgattca ctttttcaaa atacttttcg ttagaaagga ggtgaggaaa catgggtatt
    15361 tttgaaaagt tttggaaacg aaacaagcca agtaagtcaa tcaacatgct gagtcattca
    15421 gatttagggt tgtcaaacct tatggattcg tatgtacctt tggccagaaa tccagatgtg
    15481 gtgacagcgg ttaataagat tgctgatttg gtctctaata tgaccatcca tctgatggaa
    15541 aacacggata aaggtgatat cagaattcgt gacggacttg ctagaaagat tgacattaat
    15601 ccgtgtgaac acatgacaag gaagtcatgg attttcaaga ttgtgcgcga tttgctttta
    15661 tatggcgatg ggaactctgt cctacatgtg gaatatgaac ctgttacgga ttatatttct
    15721 aatctaagac catttccgat gagagaggtt tcattccaaa cagataagga ttcctatgta
    15781 atctcattta ggggtgaaga atattcccct gatgacgtag tccacttcgt catcaatcca
    15841 gatccagata ttccatacat tggtactggt tttagggtga cgttgacaga tgtggttcaa
    15901 agtttgaaca tggctaccaa gactaaaaaa agctttatga acgggaagaa tattcctagt
    15961 cttatcgtta aagtagactc gtctagtgct gaactagatt cggagcaagg gcgtgagcgt
    16021 atcgctgaga agtatttgag tactagcagg gttggcgctc catggattgt tccagaggca
    16081 ttgctggaca ttcagcaggt aaaaccgctt agtctaacgg acatcgcttt aaacgagtct
    16141 gtggaattgg ataaaagaac agttgcaggt ctattaggag tacctgcttt tattttgggt
    16201 gtaggagagt tcaacaagac agagtataac aactttgtaa atacgactgt catgagtatt
    16261 gctaccacta ttactcaaac actaaccagg gacttacttt tgtctagcaa tcgttacttc
    16321 aagctaaatc ctcgttcact cttctcttac aacattacgg agttgtctgc tgttgctcaa
    16381 caaatggcaa acagtgctgc aatgcgtcgt aatgagtgga gggattggct agggatggct
    16441 cctgatcctg agatggaaga gttgattgtt cttgaaaact ttctccctca ggagaaacta
    16501 ggagatcaaa ataaactgaa gggaggtgag gaagagaatg caaaagcgaa atagctatcg
    16561 tgccactcaa tttcaaacga gagaagaaga atctggagac ttggtgttga gtggctattt
    16621 tataaaattt gatgaagaga cggagttgtg gcgtggttat catgaagtaa tcaagcgtgc
    16681 tggtgttgag aaagctgtca cagacgctga tatcagagct ttatttaacc atgatgatag
    16741 ccttgttctc ggtcgaacag gtaacggaac tctgacactg ggtgttgatg atgttggtct
    16801 ttttggagat atcatcatta acaaggatga tcctcaagct gttggggcct atgcccgtgt
    16861 caagcgtgga gatgttatag ggtgtagctt tggctttata ccggtaaaaa tcgaaacgga
    16921 agaacgtgaa gatggttcat atttggacac tgtcttagag ctagaaatct ttgaagtgag
    16981 tccatgtact ttcccagcct atccacaaac ggaaattgct gcacgacaaa aagacttcga
    17041 aagtcagaag cgtgctaatc gtgaagcgct agacaagcgc aagaaagaaa ttaaggagaa
    17101 atttaagcta tgaataaggc attaatcttt ggtgctcgta tgcgagcaaa agcaactaag
    17161 gtagttgagt tggaagaaac tatcgaagaa ttgaacaaac gttcggttgt tgagttagaa
    17221 aagttggatc gtgctaaaaa tgatgaagaa gtttcagcag ttgaaaagaa ccttgaagac
    17281 atccaaaaag aatcggatga aaaggaagca gaaaaagaac aacttgaaaa agaaatcgaa
    17341 gatttgaaaa atcaagttga agaactaaat cgtaaagcac cgacttaccc aagtcaagaa
    17401 aaacgtggag gacagaaatt ggaacaacgt gacgcaatcg ctaaatacat tcgtactggt
    17461 caaactcgtg acattgtagg cttgaaaact actgattcag gaagcgcagc tttaatccct
    17521 actgaagttt tgaaacctca ttttgttaat aaaacacgta atccactttt ggatcttgtg
    17581 gaacgtgtga aagttaatag tggatctggt aaatatccag ttatcaagaa aacggatggt
    17641 gtaatggttt caacagatga attgaaatca aatccagaac tcggaaaacc agcaatcagc
    17701 gagattgatt attcaatcaa gacttaccgt ggatatgtcc ctgtgtcaca agaaatgatt
    17761 gacgacgcag actatgacat catgtccatt gttgaagacg aagtgttcaa tcaaggtgaa
    17821 aacactgaat tgtcattagt tacagctgtc ctcaaaacag ctacccaagc agatgcggct
    17881 ggatttgatg gtattaaaga tatctacaac aagaagctta aatcaattta taaagcaagc
    17941 atcgttgtaa ctaagtcaat gtttgctgcg cttgacaagg tgaaggacaa agatgggcgc
    18001 tacatgcttc aaactgatgt ggcttcacct actggctatt catttggtgg gaaaacaatc
    18061 tacaaagtag atgatacagt gtttggaaac gaaggagata tgaaattctt catcggagat
    18121 gttactgagt tcgtcaaaga gtttgaccgt tctcaagtat ccgttaaatg ggtgaacaat
    18181 gacatttacg gacaattgct tgggcttttt atccgtttgg atattaagaa agcagatgaa
    18241 gaagctggat tcttcggaac atatactgat gttgtagctt aaggaggtag cgtatgagct
    18301 ataaagtaat ccgtcctttt aaggacttac gagacccaaa gaaacattgc tataaggttg
    18361 gggataatta ccctagagtt aagcacaaac ttgataaaga gtttgcggag actctgctaa
    18421 atggggcaaa tagtgcagga tctatctttt tgatgaatgt agaagataag gatatgtcga
    18481 aagatgacac attaactaca gaagtagaaa aggaagaaac aaaggtagta gatgggaaac
    18541 ctgaagaggg agacgaggag tagttatgga caatgttcaa ttattagaat tgcttaagct
    18601 aaaattgggt atagcaacaa aactacgcga taagcccttg gagaaaatca tcgaagctgt
    18661 caaaacagaa ctagaagata acttaggagt tctacttgac ttagacagct cagaagacca
    18721 aatgtttgta gttgattttg cagcctttcg ctatgagggt ggggtggata tgccacgtca
    18781 ccttcagtgg cgactgcata atttacagat agcatcaaag aaagaggtca agaatgtgga
    18841 atcatgaaat cacactgatc tctaaggaag tcacaggtaa ggataaacta ctacaaccaa
    18901 tctctgaaga tgttgaagtt actctcctat gtcgcaaaaa gagggtcact cgctctgaat
    18961 tttatcaggc gaatcaggta gggcttaaac cgagcttggt cgttgagatt cgaaattttg
    19021 agtatgagaa tcaagagctt gcgaagttcg aaggcaagca atatcatatc ttgaaaacct
    19081 atcctattga ttctgaaatt ttagagttga ctttgtcaga gatgttgaaa tgagcttaac
    19141 aagtgattta gcgaatgaaa ttgcaaaggc aatggcagag tactctgctg aggtagaaga
    19201 taagattgac ctgattgctg aggacgttgt aaacgaagcc gttacggaat taaaagcgac
    19261 tagtccaaaa cgtcatggaa agtatgctag aaattggcgc ttcaagaaaa atgctaaggg
    19321 gtcatacgtc atctacaacg cagctccaac ctatcgttta actcacttac tagaaaatgg
    19381 gcatgttttg agaaatggcg gtcgtagtcg ggcatttcca catattaaac ctgttgagga
    19441 gaaagttaaa gaaaactttg agaagcgaat caaggagatt ggaaaatgaa gctatcagac
    19501 tttgcagcta ttttggaaca ggcaaacttg cctgtcactt atcgagcgtt taaaactggg
    19561 aacgctcctg acctacctta tctggtctat tatgaatcaa ttccagccat caatgcagct
    19621 gacaacacgg ttaatcatca gattaagagc gtgacagtag agctagcttt tgagagtaag
    19681 gatgaagatt tggaagaacg tctggaagag ctgtggacaa cccacgagct ctttttcgat
    19741 gttcaagaag aaacatttat cgagattgaa agactctatg tcaagtctta tacagtctat
    19801 ctatactaag gaggaatgac atgaatcaag aaaataaagt aacttttggt ttaaaaaatg
    19861 ttcacgttgc gccaattaaa tcaattggtg cagatggagt gattgcttac gatgaaattt
    19921 tccgctttcc tggagcaatg gaattgacat tggatccaag gggtgaatca ataccaatca
    19981 aagcagacga tatcgattat cacttcatga actcaaacga agggtatgaa gggaaattca
    20041 aaatctctca cattattgaa atgtttgcga ctaagatttt gggtgaaatc aaagatgctc
    20101 agacgggtgt tttgactgaa aaagctgatg cagaattcac atcatttgcc ttgatgtttg
    20161 aattttcagg ggacaagaat aaaacacgtc acgtccttta ctactgttca gcgagccgtc
    20221 caggcaatgg ttcaaaaacc aaaaatggta caaacgtcaa cgagcgtgaa cttggcttta
    20281 aagcaagtcc tcgccctctg gattcagttg ttaaacgttc tatcacatca gctgataaca
    20341 aggaaattta tgacaactgg tttaagaaag tgtatgaacc tactgcggtg gcagcttaag
    20401 gagaaaatct atgcgtaaaa tcgtttgggt tggcgttcag gagtatgagt tggggaccaa
    20461 tggctatact cctatcgcct acaagcaaca atttgggaaa gattattttc aagatttgtt
    20521 ttcaatgttg aaaaatcaat cattcatgaa tgaattgaac aagctggaaa ctgacaaaga
    20581 gttgactgca actgatattg acatttcgat gctgtcagat tttgatatga catttttcaa
    20641 ccgtcttttt tggacctttg ctaaatctgc aaatcctcac atcaagcctt atgaacaatt
    20701 cttcatggaa atggaagtct ttccgattca ggaagttggg cctgtattga tggaaatgct
    20761 gaatgcgagc atgacgacaa aaaagcacca gatgaatcag aatcagctag cgaagaaatc
    20821 ttcacagtag aatcctactt atcttgctgt aaagaaactg gtctttctat tgatgatcta
    20881 aagcacattt caatcggaat ggctctggat tatcagacgg attatgtgaa tctacggagc
    20941 gaggataagg gtggcgaacg gaaagccacg caagctgatt ttgacagttt ttaaagaaaa
    21001 atgagtgctg agagagcgat tctgagacca agttccttgg tctggctgca ttatcagtcg
    21061 tagaagttct ctcagcgctt ttctattttt tgagaaagga ggaaatatgg caggaaatat
    21121 caaaggtatc aaaattgaaa tcgatggcga cacgcaaccc ttgcagaagg cgctgaaaaa
    21181 tgtcaataag gccgctactg atgcaagtca ggagttgaga cagattgaca aagccttgaa
    21241 atttgataca gggaacgtaa cgctcctgac tcagaaacaa gaggtcttac aaaagcaagt
    21301 ttcgacgacc aaagagaaac tagaaacctt gagacaagct cagtctcagg tggaacagca
    21361 attcaaaaat ggcgatatcg gtgctgacca gtaccgtgct ttccaacgtg aagtagaagt
    21421 tactcaaaac gtcctaaaag gatatgaggg taagcttgca aatgtgaatc aggcgcttaa
    21481 tgagaatggg agtgctactc agaacaacaa gaaccaatta aaagagttgc aaaatgagca
    21541 gagtcaactt gcttcagaga tggtaaaggt gacaagctca ttcaaactgc aagaaagcgc
    21601 tttgggttca aatgctagtg aagccgagag aaatgctctt gctcagaaaa agattggtgt
    21661 tcagtctgag attgtaagta aacagatttc aaatctagaa cagcaattgg aaatcactaa
    21721 aaaagaattt ggtgagaact ccacacaagc caacaggatg gaagctgagc taaatcaggc
    21781 taagacagca ttcaatcatc tcaatgatga gatgaaagga acaaagtctg ctgctgatag
    21841 cactcaagaa agtttaagtg aaatctcaag aaatttaaga gcagaactac ttcaacagtt
    21901 tagtgagaag ttgagtgcta tttcagaaaa gcttgtggaa gtaggaaaag aagcgttaga
    21961 agcagctgct caaatgcaag ctagtaatgc tcaatttact accgttttcg gagatatgga
    22021 aacccaagca agagaagcgt tgaatgctat tggtcaggaa atggatattg ttccagagcg
    22081 attgcaaggt tcgtttacac agatggcttc atttgccaaa acttcaggct tggacaccgc
    22141 tgaagcattg gatcttactt ctcgtgcaac tagggcagct gcagatggtg cagcctttta
    22201 tgacaagtct attgagagcg tgacagaaag cctacaatct tttttgaagg gaaactttgc
    22261 taacgatgcg gctcttggta tctctgcgac agagacaact aggaatgccg ctgcaaatga
    22321 attgtacgga aagtcattca aggacttgag cgaagcgcag aagcaattga ccttgcttca
    22381 gatggtcgaa gacggaaata aactctcagg agctcttgga caggctgcaa gagaatcaga
    22441 cggcctagaa aacgtgatgg ggaatctgaa acaagctggg accaatgcat tatctgctat
    22501 tggtcaacct cttctagaaa tgatgattcc tgtttttcaa accttggcaa cgattgtgaa
    22561 aggtgtggcc gagctgttca attccttgcc tgatccagta aaagatttca ttgtcatctt
    22621 aggtgtggct ttgacaattg taggagcctt agcccccata ttcttaaccc tgcaagctgt
    22681 gtttatgtcc tcatttggcg caatgattgc agcagcatta ccaatcattg gaattatttc
    22741 aggagttgta gtggccatag cagcgattgt tgctattgtg aaatatctct gggaaactaa
    22801 cgaaggtttt cgagatgcgg tcacgaccgt ttggaatgcg attcttgaag ttatcaatgc
    22861 agtcgtatca gagatttcta attttgtcat gagtatcttt ggaacggttg ttgcttggtg
    22921 gacggagaac caggaactta ttcgagcaag tgctgagact gtctggaatg ctatctatac
    22981 ggttataagc acaattctgg aaattttagg tccactcatt caaactggtt gggataatat
    23041 ccaacttgtc attacaacag cttgggaaat cattaagact gttgttgaga ctgcaataaa
    23101 cgttgtcctt ggtatcattc aagcagttat gcagatcatc aatggtgatt ggtcaggcgc
    23161 ttgggaaact attaaggggg tattctctac tgtatggcaa gctatccaaa gcattgtcca
    23221 gaccattttt tcagctatcc agagctacat ttcaaatgtt ctcaatggta tttcaggaac
    23281 tgtatcaaat atctggaacg gcatcaagga tactgtctca aatgtgttaa atggtatatc
    23341 tggcacagta tcaagtgttt gggaaggtat taagagtacc atctcaggag ctatcaatgg
    23401 tgcaagggat gctgtatctt cagctattga agctatcaaa ggattgttta actttagtat
    23461 cagctggcca catatcccac tacctcactt ttacgtgagt ggttcggcca atccattaga
    23521 ttggttgagt caaggtgttc caagtattgg aatcgaatgg tatgccaaag gcggtatcat
    23581 gacgaaacca actatctttg gaatgaatgg taataacata atggttggtg gtgaagctgg
    23641 gaatgaagca gtattgccac ttaacgacaa aacgcttgga gccatcggtc gaggtatcgc
    23701 tcaaactatg ggtggaactt caccaaccat taacattacc attactggca acactgtcag
    23761 agaagaagcc gacatcagtc gtattgctga tgaggtggct cagcgtattg ctgacgagtt
    23821 gcaacgtaag acacaattga gaggagggtt tacatgataa agcataatga gcttgtgatt
    23881 gacggtgtga gaacatcgtc ttttccgttt aaagtcattg tccatgattc tccttcaatt
    23941 gctctgggag agagcaagac agctcttttg gagcatggtg gtatcagtgg agcaatcgtt
    24001 cagacgaaca aacataggga attggtcaag aaaacctata cgatttactt ggtcaaacct
    24061 actgaagaac agatgaacca atttatgagt ctgtttatcc gtgaaaagtt ctggctagag
    24121 agtgagcgag tcaaaacaac tcgtctttgg tgctataagg tcaatgtgag cgaccttgaa
    24181 gaagtgcaac ctggtcttta catgaccaaa gcaaccttca cttgccatcc tacaaaatac
    24241 tttaaaggct ccgatacaca gagattgaca agaagtggaa ccttgaccgt tcaaggttct
    24301 gctcttgctt ttcctaaaat cacaatcgtt ggtcagagcg ctgttgagac ttcatttaca
    24361 atcgctggtc aggtcattag gcttgaaaaa ctctcagaat cgcttgtgat ggttaataat
    24421 cctgacaatc ctagctttaa aacgacaaca gggaagccag tcaaatggtc aggggatttt
    24481 atcacagttg atccagcgaa agttaagaat attggggttg ttttaggccc aggtattcaa
    24541 tcgattgaaa tcgaaacggt ttgggggtgg gcataattgc tttatctact taataaagat
    24601 gtaagaaccg ttcgatggaa cggggagcca cttcatgaag cgacttcggc gattgttaaa
    24661 gagatcatga atggtgattt caccttaact gtgaaatatc ccatttccga ctctggtatt
    24721 tatcagctca tccaagaaga tatgttgata aaagcgccga ctcctgttct tggtgcgcag
    24781 ctatttcgca ttaagaaacc tgttgaatac aatgatcatc tggaaatcac agcctatcac
    24841 atttcagacg atgtgatgca acgttctatc acaccagtaa gtgtgactag tcagagttgt
    24901 ggcatggctc tttctcgcat ggtacaaaac accaaaacgg ctcttggaga cttctcattc
    24961 aacagtgata tccaggaccg taggaccttc aatacgactg aaacagaaac cctatactct
    25021 atattgctgg acggtaagca cagcattgtt ggtacatggg aaggcgagct ggttcgtgat
    25081 aactttgcga taactgtcaa gaagagtcgt ggggagaatc gtggtgttgt tattacaacg
    25141 cacaaaaatc tgaagaacta ccaacgcaca aaaaacagtc agaatgttgt cacaagaatt
    25201 cacgcaaagt cgacttttaa acctgaaggt gctgaaaaag aaacgactat cagagtgact
    25261 gttgatagtc ctcttatcaa ctcataccct tatatcaatg aaaaagagta tgagaacaac
    25321 aatgctaaaa ctgttgaaga gttacagaag tgggcacagt ctaagttctc aaatgaggga
    25381 attgacaagg tctctgatgc tatcaagatt gaagcttatg aacttgatgg gcaagtggtc
    25441 catatgggcg acacggtcaa tctcaagagc tggaaacata atgtcgatgc attcaagaaa
    25501 gctattgctt atgagttcga tgccttaaaa gaagaataca tctctctgac tttcgatgat
    25561 aaggcaggta ttggtggttc tagagcttct ggtggcctat ctagcgcagc cgatgcaatt
    25621 cttggagtga cagaatctgc acaagaaatc gcccttgaaa aggctcttca aaatgctgac
    25681 ttagactttg atcataaggc tggattgctt agacaggaaa tttctgacga tattgaactt
    25741 gccaaggcca aggcggaaga agtcaagcaa gaactgtctg acactatcaa tcagcgcttt
    25801 aatagctttg acaacgggcc attgaaagaa gctaagcgca gggctgaaga agccttgaga
    25861 aacgctggcg caagcagctt actcgctcag gaagcgaagc ggattgggct ggattctgtc
    25921 gctagacttg aagagtttaa gtcgcagact acgagcgcac aaacggctct gtcaggtgac
    25981 ttggacgctc tgaaacggac tatcgtgaat gatattcgac cgaagcaagc acaggttgaa
    26041 gctgagattg ccaagcaagt tgaagcactt gttcagacca aaaaagaact ggctggcgca
    26101 agtaccctgc ttgcacagga agctaagcgg attgagctgg attctgttgc tagacttgaa
    26161 gcgtttaagt cgcagactac gagcgctcag acggctctgt cgggtgactt ggacgctctg
    26221 aaacggacta tcgcgaatga tattcgaccg aagcaagcac aggctgaaac tgagattgcc
    26281 aagcaagttg aagcacttag ccggactaaa aatgaactgg ctggcgtgaa gtcagcgcaa
    26341 gcgacgtatg aggagacgac gactcgtaga ctgtcagaac tgaccaactt ggccaatggt
    26401 aaagccagca agtcagaact tacgcaaaca gctgaggagc tggctagtcg gattgcgagt
    26461 gtgcaggcag gtagttcacg gaattacttc aggaattcac gttcaagaac gttcacaaca
    26521 ggaggtcaag cggtatacga ctatcgaaca ttcatagttc ctgatttctg gaagaacagt
    26581 gacaggttca agcgtgatta tgttcgcata tcttttgatg tgactttccc tgtcgcccta
    26641 gtaaatgaca tgcctgctat ggtgcatttt agtgctcatc catggtatgc ctacagaaac
    26701 ttaattttta aaggtggaac tgtcgaacgc caacattttg agtttacgat tgacttgtct
    26761 agttcttctg aggactatca gactaataat gtgttcattc gttttggtac taattatgga
    26821 tttcctgctg gtctgcaggt cgtcattgag aacgctatgt tatcggttgg taattatttt
    26881 ccagcctatc aaccagcgta tgaagaccaa gaagaccgtg tctcagtagt cgaatccaac
    26941 tttaagcagc gtgctgattc actcgacgct ggtgtaagcc gtctgactga aggccttaga
    27001 actaaagccg atatcagcgc actcaacgtg actgctgaaa atattaggca gtcggtgaag
    27061 agtcttgaga cagacacgca gaacaaacta aatcagaagt tgagtcaggt tgaatttgag
    27121 gtgcgagctg gctctatccg tcaggaaatc ctgaacgcaa ccaaggataa agccagcaag
    27181 tcagaactca cgcaaacagc tgaggagcta gctagtaaga tagcgagtgt gcaggcatcc
    27241 ggtcgaaatc tattcttgaa ctcactattc aagcaggata ttccaaaaac aggaatttgg
    27301 acaacgagta catatacggt tactatcgat agcgaaagta agtatcttgg acacaaggct
    27361 cttaaaatta taggtctgaa tccatctggc cgtgatggag gtaatcccaa ggttacttat
    27421 ccagctctgg gtcaattcgg gaaagtaatt cccggaagta cgactaatca agatgtaatc
    27481 attagttttt atgctaaggc aaataaaaat ggaataatgc taagatctcg attagggaat
    27541 atcggatata aaactggaaa tgtgacattg tcgacagaaa ttaagcgata tgttgtccat
    27601 attccaaaag gttggacaaa cgaatccaag cggaccacaa atgaatggtt gttcaatttc
    27661 aaccaagaag gaaccgtttg gatttggatg ccgaagtttg aaataagcga tgtagatact
    27721 tcttattcag aagctcctga agatatagaa ggtcagattt caacagttga atcgaccttc
    27781 aaacaacgag ccaactcgct cgaagctggt gtaagccgtc tgactgaagg ccttagaacc
    27841 aaagccgata tcagctcact caacgtaact gctgagaata tccggcaatc tgtgaagagt
    27901 cttgagacag acacgcagaa caagctaaat cagaagttga gtcaggctga atttgaggtg
    27961 cgagctggct ctatccgtca ggaaatcctg aacgcaacca aggataaagc tagcaagtca
    28021 gaactcacgc agacagctga ggagctctct agtaagatag cgagtgtgca ggtcgggggt
    28081 aggaattata tccggggtac aaagcgcatg atgctagcca gaggattgtg ggcatcaggt
    28141 acttttagac cgtcaggcgc tgggacggca aagacgattg atgtatcaga cagtccagca
    28201 actggctttg ataaagcgat acgattgacc tcaagcaatg ctagagacca aataggtatt
    28261 gctcaagatg gattttatat ctcgcaaggc acatacacga tgtcttgttg ggtcaaaggc
    28321 agaagaggtc aaaaggtcaa gctacaaact tattggcaag tcaatgataa ttcgggtatt
    28381 tcgcccatct ttacattaaa ggatgaaaat tggacaaagc tatcgtttac tagcgctaga
    28441 aatagggctg gagtcgcatc aattggctat gtgtatctcg taaatgctga ggtcggagaa
    28501 tatttagatg ttcttgcgcc ccagctggaa gacggaagtt tggcaacaag ctcaaaagaa
    28561 gctcctgaag atatagaagg tcagatttca acagttgaat cgaccttcaa acagcgtgct
    28621 gattcactcg ccgctggtgt gaatcgtctg actgaaggtc ttagaactaa agccgatatc
    28681 agcgcactca acgtgactgc tgaaaatatt aggcagtcgg tgaagagtct tgagacagac
    28741 acgcagaaca aactaaatca gaagttgagt caggctgaat ttgaggtgcg agctggctct
    28801 atccgtcagg aaatcctgaa cgcaaccaag gataaagcca gcaagtcaga actcatgcaa
    28861 acagctgagg agctagctag taagatagcg agtgtgcagg catccggtcg aaatctattc
    28921 ttgaactcac tattcaagca ggatatttca aaaacaggaa tttggacaac gagtacatat
    28981 acggctacta tcgatagcga aagtaagtat cttggacaca aggctcttaa aattataggt
    29041 ttgaatccat ctggccgtga tggaggtaat cccaaggtta cttatccagc tctgggtcaa
    29101 ttcgggaaag taattcccgg aagtacgact aatcaagatg taaccattag tttttatgct
    29161 aaggcaaata aaaatggaat aatgctaaga tctcgattag ggaatatcgg atataaaact
    29221 ggaaatgtga cattgtcgac agaaattaag cgatatgttg tccatattcc aaaaggttgg
    29281 acaaacgaat ccaagcagac cacaaatgaa tggttgttca atttcaacca ggaaggaacc
    29341 atttggattt ggatgccgaa gtttgaaata agcgatgtag atacttctta ttcagaagct
    29401 cctgaagata tagaaggtca gatttcaaca gtagaatcga cctttaaaca acgagccaac
    29461 tcgctcgaag ctggtgtaag ccgtctgact gaaggcctta gaaccaaagc cgatatcagc
    29521 tcactcaatg tgactgctga aaatatccgg caatctgtga agagtcttga gacagacacg
    29581 cagaacaagc taaatcagaa gttgagtcag gctgaatttg aggtgcgagc tggctctatc
    29641 cgtcaggaaa tcctgaacgc aaccaaggat aaagcagata agactttagt tgtatctgaa
    29701 gctgggaaat tgcgtgaaga attttcaaaa atgaaggtgg gaggccggaa tctatggata
    29761 aaatccaaga cggttggagc tgtaattgaa aaattacctg aaaaccacgt cacaggtcaa
    29821 aaagaatgct ataggctaga gaacaactct actttaacgt tcaaccttga accagatttc
    29881 agctcaaggt tgtaccaaaa agttactttt agcgcttgga tcaagtacga aaatgtagtc
    29941 caaggtcgaa atttttggaa tgtatttaat tgcttcaaac attatctttt tagaaaaaat
    30001 agtgagaccg gagtacagag tggtccagat tatgctacgc ttggtatgta taaaggttcg
    30061 gcagattgga aatatattac attcacttat gactactctg aaaaaacaaa ttttgatcaa
    30121 ttgaagacat cattgcgatt caatcttgaa ggtgctacaa gcggtacagc ttgggtaaca
    30181 ggaatcaagg ttgaaatcgg tagtgtggcg acggactgga gtcctgcgcc tgaggacgct
    30241 gatggtctca ttactgaagc taaggctacc tttgagcgga cagctcaggg cttgcgaacc
    30301 gatttatcag ctattcagga atatgtaaat aaaaacggtc agcgacagga agccctacag
    30361 cgctatactc gtgaggaaag cgcgagacaa gcgacagcag tccgtgagct ggtcaatcgt
    30421 gatttcgttg gtaaggctac ttatcaagaa gatgttaagg gtatcaatca gaggattgaa
    30481 gctgttaaaa ctagtgcgaa taaagacatc gctagtcaaa tcgctagcta tcgtcaatct
    30541 gtagatggta agttcacgga tatttcaagt cagataacta cttataagca agatgtgggc
    30601 ggtcaaatca gtggtctatc aaatagactt acaagcagtg agcaaggaac cactactcag
    30661 atttcaaata tttcaaatcg gataaacagt aataaacaag gcacagataa tcagatttca
    30721 aatttaaaga ctcaggtcgc tacaaacaag gataatgctg aacgacaaat gggtagaata
    30781 tctgatcagg tttctgcaaa caaagcgaat gctgatagtc aatttgcgaa tgtgaccaat
    30841 caactagcgc gaaaagtaga gactactgac ttccagcgtg ttaaggaaac cagtaaactt
    30901 tacgagcgga ttttgggcaa tactgaaaat ggaattgcgg ataaagttgc tcgcatggct
    30961 ctgaccaatc aactgtttca ggttgaggtg gctaaaaatg ccagcaatgg tcaaaatttg
    31021 ttgaaaggca caaaagactt ctcaggggat tggaaaaaca agggtgcaaa ttggaaaaag
    31081 cacgctgaaa aatacaaagg tgttgatgtc ctatttaaaa ataattcgtg gaatggcgtt
    31141 ggacaagaga ttgatgcaaa aattggtgaa gtctatacat tcagcttatg gatgaagagc
    31201 gactggaaga atgatacagt aaatttctat gtaaatagaa atggatctgt tgagaaaggt
    31261 tggggtgttc catctgaaac atcggtcgct ataacaagtg aatggaaacg ttactcattt
    31321 accttcaaaa ttactgtgga cggcttcatc tttcctcgtg tagaacgact taatcaaaat
    31381 acaaatctat atattgcagg tcttaaactt gaaaaaggat cgtatgcaac accgtacacc
    31441 gaagctcctg aagacacgga cgaagcgatt cgctctgttc aaagccaact aactggctca
    31501 tgggcagttc aaaacatcaa ctcggctgga gatatcatct ctggaatcaa tcttggcgcc
    31561 aatggacata accgctttgt tgggaaattg acccacatca ctggagagac cctgattgac
    31621 agagcagtca tcaagtctgc catggttgat aagctcaaaa cggccaattt tgaagctggt
    31681 tcggtcacga ctacgatatt agacgctgaa gcggtaactg ctgagaagtt gaaagttgac
    31741 gatgcgctta ttagaaaatt aactgcaaaa gatgctttta ttgaccgact gacatctgaa
    31801 cgtatcttct ctactaaggt tgagtccgtc atttctagct caaccttcct agaagcctat
    31861 caaggccgaa tcggtggatt cacacttggt caatttgacc agggtggcgg tcgctggatt
    31921 tcaggtgtca atcagttctc tgttggtatg gggaatggtg ccgggtatgg agtccggaca
    31981 gccttctggg cgaactgggg aaataattgg aactatgccg gacctaaagc atggaacgtc
    32041 aatactgatg ggaaaatgta ctgtaggaat gaagtcggtt tttatgatca agtggatttt
    32101 tcgaattcat cgagagcaaa cttctatggg aatactactt tttctcgttc tcctgtgttt
    32161 tcaaatggta tcgaacttgg aagtaaagat gtgcttggtg atggttggaa tcccaaaggc
    32221 ggaaggaatg cggttgtttg gtggaatcag gtcggtagcg gtagcttgaa gtattggatg
    32281 gaacaaaaat cagatagacg cttaaaagag aacatcacag atacagctgt gaaagccttg
    32341 gataaaatca acagattaag aatggttgca tttgatttca tcgaaaataa gaaacatgag
    32401 gagattggtc taatagctca agaggctgaa accatcgttc caagaattgt ctcacgagat
    32461 cctgagaatc cagatggcta tctacatatc gactataccg ctttagttcc ttacttaatc
    32521 aaggctattc aagaattaaa tcaaaaaata gaaaaaatgg agaaaacaat agcatgaata
    32581 acaacatgtt gaccaatatc gcacttaaag caattcagga gcttgctctt gaaaatagaa
    32641 aacgaacaca cagattggag aacttagaaa atgaacacag aacagcttaa ccaagcctta
    32701 caaatgacaa ttagtgaaat gtcaacaact tcaacaaatt cgatgattac aagtaatatc
    32761 ttgagtattc agttggatga gcaaagggaa gagaatcaaa gacttcaagc acgagtggat
    32821 gagctggaag ctctgcttga tgaacaaact aaaccagcag acaaaggaga atagacatgg
    32881 cagaaacaaa tcaaaacaca gataacttac tagaccttac aaaaatcaca gaaccatttg
    32941 atcttgcgag tgctttgcgc tacatgaaag aaaatggaga gttcattcgt tgcaagaatg
    33001 taagcgatga cttctatatg tatcgtgacg ttcaaaaacg tcctgtgatc gtaaatggcc
    33061 gtcgccaatt caaggatgtt gaaaccgttt gggcgttcaa ccagtggggt ggtacaatcg
    33121 caacaatcaa cgtagccgtt ctgttgaatc atgaattcta tatcatgaaa tttgatgcag
    33181 agggcaatcc tgactggacg gatccaacgg taaaacctaa agaataggag gtgtgtatgc
    33241 aaattgaatt tttcaatttt ctaagaagtg tcgtacagac tgaagatggt ttggtcttgt
    33301 acgctctaac actgattgtc tcaatggaaa tcattgattt tgtcacaggg acgattgcgg
    33361 cgattatcaa tcctgacatc gagtacaaga gcaaaatcgg cattaacggg ctccttcgta
    33421 agatttcagg ggttctctta ctgatgatcc tcattccggc gtccgttttg ttgcctgaaa
    33481 agacaggttt tgcattcttg tactcaatct atctcgggta catcgcattt acttttcaat
    33541 ctctcattga aaattaccgc aaattaaaag gaaatgttac tctttttcag ccgattgtaa
    33601 aagcatttca gcgattactt gaaaaagatg atgatacgaa aaaaggagaa taacaaatgc
    33661 aacaaattac tgaaatcatt actaatggag caatcagcat cctagtcgtt ttggcagggg
    33721 ttgtagttag ggcagtcaag gactacctgg ttcaaaaagg tggagaaaag accatcaaaa
    33781 tcgttgaaat cttggccaaa aatgcagtaa atgccgtgga gcaggtagct gctgaaactg
    33841 gctacaaggg agatgaaaaa ctggcacagg ctcgcgctaa agtccgtgct gagcttacaa
    33901 aatacaatat tagcatgact gacaaagact tagacacctt tgtggagtca gcagtgaagc
    33961 agatgaatga cgcatggaaa ggacgatagg gaatggatat cgatagaaac agactacgta
    34021 caggcttgcc acaggttggg gtgcagcctt atcgacaagt acatgctcac tcaacaggta
    34081 accgcaactc aaccgtacag aatgaagcgg attatcactg gcggaaagac ccagaattag
    34141 gttttttctc gcacgttgtt gggaacggtc gcatcatgca ggtaggacct gtgaacaacg
    34201 gaagttggga tgttgggggc ggttggaatg ctgagaccta tgcagcggtt gaactgattg
    34261 aaagccattc aactaaggaa gagtttatgg ctgactatcg cctctatatc gaattgctac
    34321 gcaatctagc ggacgaagca ggcttgccga agactcttga tacagacgac ttggcaggta
    34381 tcaagacgca tgaatactgt accaataacc aaccaaacaa ccactcagac catgtggatc
    34441 catatccata tcttgctaaa tggggcatta gccgtgaaca gtttaagcaa gacatcgaaa
    34501 acggcttgag cgctgcaaca ggctggcaga aaaatggcac tggctactgg tacgtacact
    34561 cagacggctc ttatccaaaa gataagtttg agaaaatcaa cggtacctgg tattatttcg
    34621 atggctcagg ctatatgctt tcagaccgct ggaagaagca cacagacggt aattggtact
    34681 actttgacca atcaggcgaa atggctacag gctggaagaa aatcgctgag aagtggtact
    34741 attttgatgt agaaggtgcc atgaagacag gctgggtcaa gtacaaggac acttggtact
    34801 acttagacgc taaagaaggc gccatggtat caaatgcctt tatccagtca gcggacggaa
    34861 caggctggta ctacctcaaa ccagacggaa cactggcaga caagccagtg ttcacagtag
    34921 agccagatgg cttgattaca gttaaataaa tagaaaggaa actttctaaa ttgttctttc
    34981 accgcaggct taggcttgcg gttttttatt tgctctgatt ctttaaaaaa gcgttttctt
    35041 gaagaataga gaggtgtttt gtcaaaaata aaaacagtga ccgaaatcac tgcttatcag
    35101 ttgtagcaaa ttcataaagt ttttctgctg tgagaagtgc cattttgtcc atgcttgttt
    35161 ttccttttct gaggtcagaa acagtagtcc atggaactcc agcaccttgg gaaatagcag
    35221 atgtagacat cgaactgtct aacaattctt gaataacttc tctcatctta tttgtccttt
    35281 ttatttttta gataaatata tacattgatt gcaattataa aaatagctat tgcactaacc
    35341 attgcatttc ctctttccat ttgataaaat agaggtgtaa ggggctttcg ccccaacctc
    35401 ttagcgttta cctttttctt tgacgggatt tcggtttacg ctttttgttt tgccttgcga
    35461 ccgttattgc ggtcactaga cttgcgatag cagttaccgt ttcaggaata ttgtctattg
    35521 ccttttcaag taacctgagc caatcttctt tgttcaactt catcaccccc tttccttatc
    35581 ttgattatat tatatcacgg tacaccgaga aagtcaagcg ttttgatgaa gtttttttaa
    35641 ttttttc
//
LOCUS       DAVKWS010000018        34722 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKWS010000018 DAVKWS010000000
VERSION     DAVKWS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.018"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            78..1970
                     /gene="pepO"
                     /locus_tag="VW852_000920"
     CDS             78..1970
                     /gene="pepO"
                     /locus_tag="VW852_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV0272091.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKAKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2093..2722)
                     /locus_tag="VW852_000921"
     CDS             complement(2093..2722)
                     /locus_tag="VW852_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0272092.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHTHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3716..5938
                     /locus_tag="VW852_000922"
     CDS             3716..5938
                     /locus_tag="VW852_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV0272093.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5968..6411
                     /locus_tag="VW852_000923"
     CDS             5968..6411
                     /locus_tag="VW852_000923"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV0272094.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6450..6811)
                     /locus_tag="VW852_000924"
                     /pseudo
     CDS             complement(6450..6811)
                     /locus_tag="VW852_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6885..7298)
                     /locus_tag="VW852_000925"
     CDS             complement(6885..7298)
                     /locus_tag="VW852_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272095.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFECLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            complement(7400..8077)
                     /locus_tag="VW852_000926"
     CDS             complement(7400..8077)
                     /locus_tag="VW852_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV0272096.1"
                     /translation="MVVGIAIASGYLNSRLDKFVDWGLWTALVPFGLISLTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKATIITYPVTFLIYTFAIKKWKASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIE
                     GNSNKEY"
     gene            complement(8254..8904)
                     /locus_tag="VW852_000927"
     CDS             complement(8254..8904)
                     /locus_tag="VW852_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV0272097.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNIDIAKQLYVEKIN"
     gene            9016..9858
                     /locus_tag="VW852_000928"
     CDS             9016..9858
                     /locus_tag="VW852_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0272098.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9934..10371
                     /locus_tag="VW852_000929"
     CDS             9934..10371
                     /locus_tag="VW852_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV0272099.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10439..10741
                     /locus_tag="VW852_000930"
     CDS             10439..10741
                     /locus_tag="VW852_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272100.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10832..11905)
                     /locus_tag="VW852_000931"
     CDS             complement(10832..11905)
                     /locus_tag="VW852_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV0272101.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            12061..12738
                     /locus_tag="VW852_000932"
     CDS             12061..12738
                     /locus_tag="VW852_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272102.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12731..13705
                     /locus_tag="VW852_000933"
     CDS             12731..13705
                     /locus_tag="VW852_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0272103.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFLLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13754..15697)
                     /gene="thrS"
                     /locus_tag="VW852_000934"
     CDS             complement(13754..15697)
                     /gene="thrS"
                     /locus_tag="VW852_000934"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV0272104.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15857..16078)
                     /locus_tag="VW852_000935"
     CDS             complement(15857..16078)
                     /locus_tag="VW852_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV0272105.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            16937..17428
                     /locus_tag="VW852_000936"
     CDS             16937..17428
                     /locus_tag="VW852_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV0272106.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGN"
     gene            17707..17976
                     /gene="rpsO"
                     /locus_tag="VW852_000937"
     CDS             17707..17976
                     /gene="rpsO"
                     /locus_tag="VW852_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV0272107.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18789..19403
                     /locus_tag="VW852_000938"
     CDS             18789..19403
                     /locus_tag="VW852_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV0272108.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19696..20445)
                     /locus_tag="VW852_000939"
     CDS             complement(19696..20445)
                     /locus_tag="VW852_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV0272109.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWVRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20578..22914
                     /locus_tag="VW852_000940"
     CDS             20578..22914
                     /locus_tag="VW852_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV0272110.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDVQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23016..23693
                     /locus_tag="VW852_000941"
     CDS             23016..23693
                     /locus_tag="VW852_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV0272111.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            23680..24477
                     /locus_tag="VW852_000942"
     CDS             23680..24477
                     /locus_tag="VW852_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV0272112.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24494..25597
                     /locus_tag="VW852_000943"
     CDS             24494..25597
                     /locus_tag="VW852_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV0272113.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            25598..25678
                     /locus_tag="VW852_000944"
                     /pseudo
     CDS             25598..25678
                     /locus_tag="VW852_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            25943..26962
                     /gene="galE"
                     /locus_tag="VW852_000945"
     CDS             25943..26962
                     /gene="galE"
                     /locus_tag="VW852_000945"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0272114.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26964..27929
                     /locus_tag="VW852_000946"
     CDS             26964..27929
                     /locus_tag="VW852_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0272115.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(27980..28186)
                     /locus_tag="VW852_000947"
     CDS             complement(27980..28186)
                     /locus_tag="VW852_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV0272116.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28238..28714
                     /locus_tag="VW852_000948"
     CDS             28238..28714
                     /locus_tag="VW852_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV0272117.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28723..29394
                     /locus_tag="VW852_000949"
     CDS             28723..29394
                     /locus_tag="VW852_000949"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV0272118.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLVVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29524..29862
                     /locus_tag="VW852_000950"
     CDS             29524..29862
                     /locus_tag="VW852_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV0272119.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29865..30494
                     /locus_tag="VW852_000951"
     CDS             29865..30494
                     /locus_tag="VW852_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV0272120.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30734..31885
                     /locus_tag="VW852_000952"
     CDS             30734..31885
                     /locus_tag="VW852_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0272121.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32198..32947
                     /gene="truA"
                     /locus_tag="VW852_000953"
     CDS             32198..32947
                     /gene="truA"
                     /locus_tag="VW852_000953"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV0272122.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFILDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            32937..33710
                     /locus_tag="VW852_000954"
     CDS             32937..33710
                     /locus_tag="VW852_000954"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0272123.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33688..34149
                     /locus_tag="VW852_000955"
     CDS             33688..34149
                     /locus_tag="VW852_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0272124.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            34373..34722
                     /locus_tag="VW852_000956"
                     /pseudo
     CDS             34373..34722
                     /locus_tag="VW852_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10029 a         6531 c         7968 g        10194 t
ORIGIN      
        1 agttaaatgg ttgttaattg cattccctag tgatttttgt taagataaag gcaaatacaa
       61 atgaaagcga gaacaagatg acacgttatc aagatgattt ttatgatgct atcaatggag
      121 aatggcaaca gacagctgaa atcccagcag ataagtctca aacaggaggt tttgttgatt
      181 tagaccagga aattgaagac ctgatgctgg cgacaacaga caagtggtta gcaggtgaag
      241 aagtgcctga ggatgctatc ttggaaaact ttgtcaaata ccaccgccta gttcgtgatt
      301 ttgacaagag agaagctgac ggtatcacac ctgtcttacc actccttaaa gaattccaag
      361 aattggagac ttttgcggat tttacagcta aactagcaga gtttgagctt gcaggaaaac
      421 caaacttcct tccttttggt gtatcgccag actttatgga tgctagaatc aatgttctat
      481 gggctagcgc tccaagcaca atcttgccag atacgaccta ctatgcagaa gaacatcctc
      541 agcgcgaaga gctcttgact ctttggaaag aaagcagcgc aaatctcctc aaggcttatg
      601 atttctctga tgaagaaatt gaagacttgc tagaaaaaag acttgaattg gaccgccgag
      661 ttgcggcagt ggtgctctct aatgaagaaa gttcagaata tgctaaactc tatcatccat
      721 attcttacga agatttcaag aaattcgcgc ctgccctacc tttggatgac ttcttcaaag
      781 cagttattgg gcaattacca gacaaggtta ttgtagacga ggaacgtttc tggcaagcag
      841 cagagcaatt ctacagtgag gaagcctggt ctctccttaa agcaaccttg attttgagtg
      901 ttgtcaatct ttcaaccagc tatttaacag aggatatccg tgttttgtct ggtgcctaca
      961 gccgtgccct ttctggagtt ccagaggcaa aagataaggc caaagcagct tatcatctag
     1021 cacaagaacc tttcaagcaa gccctgggtc tttggtacgc ccgtgagaag ttctctccag
     1081 aagccaaggc ggatgtggag aaaaaagtgg caaccatgat tgatgtctat aaggagcgtc
     1141 tgcttaagaa tgactggctc actccagaaa cctgtaaaca ggctatcgtg aagctcaatg
     1201 tgatcaaacc ttatattggc tatccagaag aattgcctgc acgttacaag gataaggtag
     1261 tgaatgaaac tgccagtctt tttgagaatg ctctagcctt tgcgcgtgtg gaaatcaagc
     1321 acagttggag taagtggaac cagcctgtag actataagga atggggcatg cctgctcata
     1381 tggtcaatgc ctactacaat cctcagaaga acctgattgt ctttccagcg gccattttac
     1441 aggcgccttt ctatgacttg catcagtcat cttctgctaa ctacggtggt attggggcag
     1501 tgattgccca tgaaatttcc cacgcctttg atactaacgg ggcttccttt gacgaaaatg
     1561 gtagcctcaa ggattggtgg acagagagcg actatgctgc cttcaaggag aaaacacaaa
     1621 aagtcattga ccaatttgat ggacaggatt cttatggagc aaccattaac ggtaaattga
     1681 ctgtatcaga aaacgtggct gacttgggag gaatcgcagc agcgcttgaa gcagctaaga
     1741 gagaagcaga cttctcagca gaagagttct tctacaactt cggtcgcatc tggcgcatga
     1801 aaggtcgacc agaatttatg aaacttttgg ctagcgtcga tgtgcacgca ccagccaaac
     1861 tccgtgtcaa tgtgcaagta ccaaacttcg acgatttctt tacaacctat gatgtcaaag
     1921 aaggagacgg aatgtggcgt tcaccagagg agcgcgtgat tatttggtaa tgcaaaaaat
     1981 ctagtcatca gaaaaaaggc atccaatcac gtgtgaaaac cttgatagga tgccttttat
     2041 aatagaaaga tttgctggat agtttactta aatttcgatt gtcatcatct tgctaaaaaa
     2101 agggattgaa ggccttttcg tgagcgatag tagtagctgg accatgtcct ggatagacat
     2161 cgtagtttgg tagggtgaag agttgggtct ggatactatg aaggagttgc tccatgctac
     2221 cagtcggaag gtcggtccgt ccgatagttt cgcggaatag agcatctccc gtcaagacta
     2281 ggtgagcatc aggaaagact agggaaacac caccgataga gtgccctggg gtcggtagaa
     2341 ccttaaaacg aaattcctct agttggtatt cttcgtgaaa gacaaaggtg tgttctgcag
     2401 gttttgtgac cacatctgcc atatcatcgt ggcgagggag accggagaga ttatcgacag
     2461 gagtgtagag ccagctggct tcgctctctg cgatatagac aggaggattg ccaaacgtct
     2521 cgcgaaccaa gtccagactc atgatatggt cataatgggt gtgggtcaag agaatagcac
     2581 agatcggttt gttgatcttc tcgattgtct gacgaatggc ttcccaatga ctaccaggat
     2641 cgacgacgat gaggtgcttt tcgccttcta gataataggt attttcatag gcaacaggat
     2701 tcacggtttt atggattttc atactagctc caatctcaaa gaatgtacta atactcttcg
     2761 aaaatctctt caaaccgcgt caactttatc tgcaacctca aagcagtgct ttgaacagcc
     2821 tgcggctagc tttctagttt gctctttgat tttcattgag tataaattaa gtatagcaca
     2881 gttagggaga ataggtaagg atttaaaatg aacttaaaaa ccagcatgac tagatgaaga
     2941 caggctggtt atcaatttat caatattgga aaggtgtcaa ttgcccttca taggttgcat
     3001 aaactccgtc attagcttgt ttgatagacg tgatgtttag agtgctaccg tagttggctt
     3061 ctcatttgtc gctatattca ctataagtta tcctactggg caggttctgg tcgttagcat
     3121 agtattgaac gagtttttta tagctttgct ggctgaatag gaacaggcag caaactccta
     3181 acattaggag agcaaagata gaaatagcag tttttttcat aggtattctc cttataaact
     3241 aaaggataga agaaagtatc ttgaggattt ttatactctt caaaaatctc ttcaaaccgc
     3301 gtcaacgtcg ccttgccgta tatatgtgac tgactttgtc agtcttatct acaacctcaa
     3361 agcagtgctt tgagcaacct gcggctagtt tcctagtttg ctctttgatt ttcattgagt
     3421 attaattcca aaatccagtc aaggtaccag tgtatgtgat acgagttcca ccgtttatct
     3481 ttcgtatctt caatggaatt ggggaaatca gaaagtcttt gggttacaat atggtttccg
     3541 ttcttctatc tctccttcta tcacctaagt ttaaaatcta acttcgtagt atattatggg
     3601 ccaataattc agaattatca agcaattttg aattattttt ttcttattct gttccaatct
     3661 agttttcata ttcttaaaaa agtgataaaa tggtaggaag aattggagag tagagatgcc
     3721 gaaagaagtg aatttaacag gcgaagaagt tgtcgcttta accaaagaat atttaacgga
     3781 agaggatgtt cattttgtcc ataaggcctt ggtctatgct gttgaatgcc acagtggtca
     3841 atatcgcaaa tcaggcgagc cttatatcat tcaccctatc caagtggcag gtattttagc
     3901 taagctaaag ctggatgctg taacagtagc ttgtggattc ttgcatgatg tggtggaaga
     3961 tacagatgcg accttggacg atttggaaag agagtttggt cctgatgtgc gggtgattgt
     4021 tgacggagtt accaagcttg gcaaggtcga gtacaaatcg atcgaggagc aattagcgga
     4081 aaatcatcgc aagatgctca tggccatgtc tgaggacatc cgcgttattt tggtcaaact
     4141 gtctgaccgc ttgcacaata tgcggaccct gaaacatctt cgaaaagaca agcaggagcg
     4201 tatttccaaa gaaaccatgg aaatctatgc cccacttgcc catcgtttgg ggatttccag
     4261 tgtcaaatgg gaattagaag acttgtcttt ccgttatctc aatccaacgg agttttacaa
     4321 gattacccat atgatgaagg aaaagcgcag ggagcgtgag gccttggtgg atgaggtagt
     4381 cacaaaatta gaggagtata cgacagaacg tcacttgaaa gggaagattt atggtcgtcc
     4441 caagcatatt tactcaattt tccgcaaaat gcaggacaag agaaaacggt ttgaggaaat
     4501 ctatgatctg attgctattc gttgtatttt agatacccaa agtgatgttt atgccatgct
     4561 tggttacgtg catgaatttt ggaaaccgat gccaggtcgc ttcaaagact atattgccaa
     4621 ccgcaaggct aatggttatc agtctatcca tacgactgtt tatggaccaa aagggccgat
     4681 tgaattccag attcgaacca aggaaatgca cgaggtggct gagtacgggg ttgcggctca
     4741 ctgggcttat aagaaaggta taaaggggca agttaacagc aaggaatcag ctattggaat
     4801 gaactggatc aaggagatga tggagctcca agaccaggct gatgatgcta aggaatttgt
     4861 ggactctgtt aaggaaaact atctggctga ggagatttac gtttttaccc cagatggagc
     4921 tgtccgttct cttcccaaag attcaggacc gattgatttt gcctacgaaa tccataccaa
     4981 ggtcggtgaa aaagcaactg gtgccaaggt caatggccgc atggttccac tgacaaccaa
     5041 gttaaagaca ggggatcagg ttgaaattat cgccaacccg aactcctttg gacctagccg
     5101 tgactggctc aatatggtca agactagcaa ggcgcgcaat aagattcgcc agttctttaa
     5161 aaaccaagat aaggaattgt ctgtcaacaa gggtcgtgag atgctgatgg ctcagttcca
     5221 agaaaatggc tatgtggcaa ataaatttat ggacaagcgc cacatggatc aagttctgca
     5281 aaagaccagt tacaagacag aagactccct ctttgcggcc attggttttg gggaaatcgg
     5341 tgcgattacc gtctttaacc gtctgactga aaaggagcgc cgtgaggaag agcgtgccaa
     5401 ggccaaggct gaggcagagg agcttgtcaa aggtggcgag gtcaaggttg aaaataaaga
     5461 aactctcaag gtcaagcatg aggggggagt ggttattgaa ggtgcttctg gtctcctagt
     5521 gcggattgct aagtgttgta accccgtacc tggtgacgat attgttggct acattaccaa
     5581 gggtcgtggt gtggctattc accgtgtgga ctgtatgaac ctgcgtgccc aagaaaacta
     5641 cgagcaacgt ctccttgatg tggaatggga agaccagtac tctagctcaa ataaggagta
     5701 tatggcccat atcgatatct acggtctcaa ccgtacagga ctgttgaacg atgtactgca
     5761 agttctttca aatacaacca agaatatttc aacggtcaat gcccaaccaa ccaaggatat
     5821 gaagtttgct aatatccatg tgtccttcgg tattgccaac ctctctacac tgaccacggt
     5881 tgtcgataaa attaagagtg tgccagaagt ttactctgtc aaacggacca acggctagtt
     5941 gtcctagctc ttactagaaa ggctattatg aaaatcatta tccaacgggt taaaaaagcc
     6001 caagtgagta tagaaggcca gattcaggga aaaatcaatc agggactttt attgctggtt
     6061 ggtgttggac cagaggacca agaggaagat ttggactatg ctgtgagaaa actggtcaat
     6121 atgcggattt tttcagacgc agaaggcaag atgaacctgt ctgtcaaaga tattgaagga
     6181 gaaatcctct ctatttctca gtttaccctc tttgcggata ctaagaaagg caatcgtcca
     6241 gcctttacag gggcagctaa acctgatatg gcatcagact tctatgatgc tttcaatcaa
     6301 aaattagcgc aagaagtgcc cgttcagaca ggtatctttg gagcagatat gcaggttgag
     6361 ctggttaata acggacctgt taccattatc ctagatacta aaaagagata agaaagacca
     6421 agcccagtcg gcttggtctt tctcatcgat cataaaatac tccaaaaaga aatcggttct
     6481 tgatatgctt gggggaagtc tcttttcagg ctttggcaga tgcgatagga agggatgaga
     6541 tgtcctagga tgaggagagt tccctgaagg aaaagtggaa tatcacttaa agacaccaag
     6601 gagagaatca tcaggctatc ccataggaga atttgtaaag caaaacgcca gaatctcggt
     6661 ctaatctggg aatagagttg actaaagtgg tcacaaagtt ggtttgaaag ataggtcaat
     6721 agcacaggat gaaagaaaat gagccaaccg ctataaatca aaatccagtc ccaagtaagc
     6781 cccccttttt aaatgtcaaa aatgccagca tgattccatc aatgacaagg agttgaatgg
     6841 ttttgatgaa gatgattttt tttcatgcta aaacctcctt ttccttagag acactcccat
     6901 aaagagatgt gtttgtcgta aaagtctgga tagttttctc ctaggtagtc agctgtcata
     6961 taataaaaag tagaatggca agcagtgata attggcataa gagatgaaaa acgctgagaa
     7021 ctaatgaagg ctaagagaac aaaaaataga aaatcaatgg tgagaacccc taaatagtag
     7081 aagcgaatct ttttattgat ttgaggataa atctcagcga attgatgttt gagtcggacg
     7141 accaagagaa atagcaggag tccttgagca aggaagaaaa ttaaaccaga gagcaagagg
     7201 cactcaaaag atgtctcgag tctagtgata aaaaatgcaa atagtagcaa atcaataact
     7261 gcaattgcta taaaatggat tctatagagt tttttcatga caagaccttc ctcttttatc
     7321 taacttcatt ctactccaaa agaatgggag ttacaactaa aatgataaaa atagcagaag
     7381 ggagattctc ttaagttggc tagtattctt tatttgagtt tccttctatg tatctaactt
     7441 cttcatcatt ccagacaaat aaagctccga ttgcattgag gatataaaag atgtatttac
     7501 cgatattggc gaagtttcct tgaataccag cttttgtcag ctgaacgaaa ttgtaaatca
     7561 accaaaagcc ccactgagtt gttagtttca atgcattcaa agcattggca atgagggaca
     7621 gtgcaaaggc aatagttgtt acgtaggcaa ggagattcat cttgccccca tatccgatat
     7681 agttggttac aaaggcaaag aggaaggcga tgatggaaat gatgatggcc gccaatttta
     7741 cctgtttttg gctcatttgg ttgggtctgc cttcttgcga agctttccat ttctttatag
     7801 caaaggtata aatgaggaag gtgacgggat aggtaatgat ggtcgcctta tttccaagga
     7861 tataatcaat agcaccggac aaaatggtat taacaatacc aaagtaattt ccccatttgc
     7921 ttaatttccc cgtgaaacga gtggacaaca tggaaatccc aacgttggtt aaggaaatca
     7981 atccaaaggg tacaagagct gtccatagtc cccagtctac aaatttatcg aggcgtgagt
     8041 tgagataacc agatgcaatc gcaatcccaa cgaccaaagc aaccccgaag aggtcaaatc
     8101 atttagatgt agcaaaaatt tttagtgatt ttttcatagg ttaaactacc tttctttttt
     8161 tcaaatactc taccaccaaa tgaaagtaaa atcaaatgat agaaataaaa ccctgaaaat
     8221 aaaactttca gggttggtag gggacttgag aaattagttg attttctcga catagagttg
     8281 ttttgcaatg tctatattca cttgtaaatc ctcgtcgtta ctgaggatag tgaaggtatt
     8341 gctgaagcca tcaaactgct tgacttggag ctggtcaccg atgtgaagtg agtgcttgtc
     8401 cagataatgg agaatgtcaa aactatcgtg cacccgagtc aggcggtagg cgccagcttc
     8461 cttgatatca gctagtggga ggttattgat ttcaacgagg agttctccct tggcaggaat
     8521 agttcctccg tgggggcagg ttttagggaa acctagcagt ttatctagtc tttccacgaa
     8581 caggtcagag acagtgtgtt ccaagacctc agcttcctcg tgaatctggt cacttgtata
     8641 gtctaaatga tgaactagaa aaacttcaat caagcggtgc ttacgataga gctcagagac
     8701 cagtttgaga ccgaggtcag tcagtagata gccacattcc ttgtccttta ggatgagatt
     8761 ttcacttttc attcgtttga tcatttcagt tacggcaggg ggagagactt gcatgcgagc
     8821 cgcaatttcc ttgttggtaa tcttatgcag gtctatgcca atttcataaa tacattttag
     8881 atagtcttct ttgtttgggg tcattcgttt cctcttgtct aatatcttta tctagtatat
     8941 caaaaaaagc tagatttctc tagctgtgac tttttatgtt atacttattg caagagaaaa
     9001 aacgaggaga tggatatgac gaaaatagct cttctttcag atattcatgg aaataccacc
     9061 gccttggagg ctgttttggc agatgctcgg cagctaggag tggatgaata ctggcttttg
     9121 ggagatattc tcatgccagg gacagggcgt agaaggattt tggacttgtt ggatcaacta
     9181 ccgattacgg ctagagtttt gggaaactgg gaagacagtc tttggcatgg tgtccgtaag
     9241 gaattggata gtactcgccc cagtcaacgc tatctcttgc gccagtgcca gtatgtttta
     9301 gaggaaattt ccctagaaga aattgaagtg ctccacaatc aacctcttca aattcatcgt
     9361 cagtttgggg atttgacggt gggaattagc catcatctgc ctgataagaa ctgggggcga
     9421 gagttgattc atactggcaa acaagaggag tttgaccgct tggtgactca tcctccctgt
     9481 gatattgctg tttatggaca tattcaccag cagttgcttc gttacgggac tggtgggcaa
     9541 ttgattgtca atccgggttc gattggccaa cctttctttt tggacgctca gctacggaag
     9601 gacttgcggg cccagtatat gattttggag tttgatgaca aaggcctggt agatatggac
     9661 ttccgacggg tagactacga tgtggcagcg gaattgcagc tggctaaaga cctgagactt
     9721 ccctattttg aggtttacta tgaaagtctg gtcaatggga tccaccatac tcatcatcag
     9781 gaatttttga gagaattggc tcagaaagag ggctgcgatc gggagttaga cgactggttg
     9841 aaaagtggta acgattgaca ttacaactaa aaagggtata ataatagaaa aagaagagta
     9901 gaggcagggt agcctcgtaa aaaggagaag aggatgcaaa ttccaagtag atttaccatt
     9961 gcgactcata tgctgataat cattgccctc gaggggaagg aaagcaaggt gaccagtgat
    10021 tttctggctg ctagtgtcgg ggtcaatcct gtcattatca gaaagatctt gtcccagttg
    10081 aagaaggcag agctgatttc agtagcgcgt ggaacgggcg gaacagagat tgtcaaggac
    10141 cttaaggata ttagtctttt agatgtttat caggcggtcg aatgtcttgg taagacaggt
    10201 caactcttca gtttccatga caatccgaat ccaaattgcc ctgtaggagc tcatattcat
    10261 gatgttttgg atcaaaaatt ggagagaatt cagttgacta tggaggcaga acttggtcaa
    10321 accagtctag aaaaagtcgt ggccgatgca gagagtcaga tgaaggatta agagggataa
    10381 atgccctctt ttttctttgg ataattatga taaaatgtag ctattaaagg agtaagaaat
    10441 gtatctcgtt ataggaattg tattagcctt tatagtgtca ttttggaagg ataatagaag
    10501 tttgtggaat ccagtattat ttttattatc cctcatttca agttattttt atctatccta
    10561 ccttttttat aaaaatggat acgagaatgt tcagttagct ttttatgtat ttgcctttgt
    10621 cttacttcct tttttgcttt ttctaagtgg catcttttta atctataatg gggtcatttt
    10681 gttgaaacga gagggacgtt ccaagcccca ttatctctca atgctctttg atttttattg
    10741 actattagat tctatttaaa aagacttaga atgcacatga aagtatgtat tctaggtctt
    10801 ttgttttgta tacatttaat tttcaagagt attatctatt tttccatgca tggtaacgag
    10861 tatcaatcaa taactgggta aggcgaccca aggtttctct ttcttctgga gtgagggatt
    10921 gagcttggac aaagagatga tgaatgtatt caagcgcctt taaggaagcc aagtcattga
    10981 tagagctaat accagcatca agaatagtgc caaagaagag gtcgaagtag agctgaagtt
    11041 cttcgtcagg gactgtggcc acccattctt taaaggttgt gtctacttgc cggctatcac
    11101 tgttggtctt atccagttga acgaagtgct tgtcctcaat ctgccaacta aaggtatcgt
    11161 gctgggcgat gccacccagg gcagtactct gaacgatgat ttggtgagca ggaatttcca
    11221 gcatcatacc gataatggaa ccttgtggaa tgaatatctt gcttctatcc attatccttt
    11281 gataacccgc agtctgtgtc aattcttgat ggagaccagg tgcatcaaat gtataaactg
    11341 ctgtgatctg attttgcaaa ctttgctcaa tttggctagc agcatagata gcgagatttc
    11401 ctcccttgga atgcccagcc agaataacct tttgcttagg atgatgggca aaaaagttct
    11461 ttaaatagcg aagggcgtgc ttttgagcag gaatttcctt catataggtc aggtggaaat
    11521 cttccttcca gccaatgata ctgtcatctg tcccacgaaa gacaatcaga taggtatcga
    11581 ggctgacacg ataagtcata gccgcaaatt gcttttgcag ttcagggtcg atgtcgttga
    11641 taaaatggga gagtttgcaa tttttgaagc gcttgtgttg agccaattca tctaataatt
    11701 gaaggcgatt tttgctagta agcatggtgg gctctcttgg aacctgaggt gctagatcta
    11761 aaagacgttg aggaagtgtg gagaccagat tatcaaagga gaggtaggtg atttctatta
    11821 aggttagaat gtctaactca ttcaagggaa ggtcgtaata agaatcatat gcgacatctt
    11881 tcagatagtc aaaaatattg gccataagag ctcctttctt tttatttatc ttagcaaatt
    11941 tccctcaaat tagctagtag cattgcctgt ttgtactggc taaaaacagg ctatttcaaa
    12001 ttcagtttca gaccatctgg catggaaaaa tctgttataa taatagaaaa ggagaagcgc
    12061 atgcacaaga ttttattaat agaagatgat caggtcattc gtcaacaggt cgggaaaatg
    12121 ctctctgaat ggggatttga agtggtcctg gtagaagact ttatggaagt tttgagtcta
    12181 tttgttcagt cggaacctca tctggtcctc atggatattg gtttgccctt gtttaatggt
    12241 tatcactggt gtcaggaaat ccgcaagatt tccaaggtac ctatcatgtt tctttcttcg
    12301 agagaccagg ctatggatat tgtcatggca atcaatatgg gggcggatga ctttgtgacc
    12361 aagccttttg accagcaggt tcttttagcc aaggttcagg gcttgttgcg tcgttcctat
    12421 gagtttgggc gtgatgagag tttgctggaa tatgctggtg ttatcctcaa taccaaatcc
    12481 atggatttac attatcaagg gcaagttttg aatttgacca agaatgaatt ccagatttta
    12541 cgcgtgttat ttgagcatgc aggcaacatc gtagcacgtg acgacctgat gcgggaactt
    12601 tggaacagtg actttttcat tgatgataat accctctctg tcaatgtggc tcgtttgcgt
    12661 aaaaagttgg aggagcaggg attggtagga tttatcgaga ccaagaaagg aatagggtac
    12721 ggattgaagc atgcttgatt ggaaacaatt ttttctagcc tatctgcgct cccgtagtcg
    12781 tctttttgtc tatctgcttt ctttggcatt tctggtctta ctctttcagt ttttatttgc
    12841 cagtctagga atttacttcc tctacttttt cctcttgtgt tgctttgtaa ccatcttatt
    12901 tttcacttgg gacatattgg tggaaatgca ggtctatcgc caggaacttc tctatggaga
    12961 gagggaagcc aagtctcctt tggaaatagc tttagcagaa aaattagaag cgcgtgagat
    13021 ggaactctat cagcagaggt caaaagcaga aagaaaactg acggatttgc tggattacta
    13081 taccttgtgg gtccatcaga taaagacccc cattgcagcc agtcaactct tagttgcaga
    13141 agtggtcgac cgccaactga agcagcagct agaacaggaa attttcaaaa tcgactccta
    13201 taccaaccta gttttacagt acctgcgttt agaaagtttc catgatgatt tggtcttaaa
    13261 gcaggttcaa attgaggact tggtcaagga aataattcgt aaatatgctc ttttctttat
    13321 tcaaaaaggc ttaaatgtca atctacatga ccttgataaa gaaatcgtga cggataaaaa
    13381 gtggctgcta gtggttattg agcaaatcat ctcaaacagt ctcaagtaca ccaaggaagg
    13441 tggtctggag atttatatgg atgaccaaga gctttgtatc aaagatacgg gaatcgggat
    13501 aaaaaacagt gatgttctcc gagtatttga acgtggcttt tcaggataca atggccgttt
    13561 gacccagcag tcctctggac ttggccttta tctatctaag aaaatttctg aagaactggg
    13621 gcatcagatt cgtatcgagt ctgaggtcgg gaaaggaacg acagtgcgga ttcagtttgc
    13681 tcaagtgaac ttagtccttg agtaaaaata gatgagaaaa agcctccaga ttggaggctt
    13741 ttatgctaga ctcttatttc tcaacgcgtg atttgttggc gatatcagct aggatagctt
    13801 gaacaaaatt atcaactgag acagtttgtg tttctttttg gccgtagcga cgaacgttga
    13861 ctgtttcgtc ttccatttct ttgtctccaa caattaattg gtaaggaatc ttgctggttt
    13921 gtgaagcacg gatcttgaac tgcatttttt cattgcgctc atctacgtct gcacggacac
    13981 cgcggtcacg gagtttcttg gccacttccc aagcgtagtc cacgtgtttt tcgttagata
    14041 ctgggatgag ggttacttgg tgtggtgcca gccatgttgg gaaggccccc ttgtagttct
    14101 caatcaagat agctgtgaag cgttccatag ttgagataac cccacggtgg atcatgactg
    14161 gacggtgatc ttcgccatca gctccgatgt atttgaggtc gaagcgttct ggcaacaaga
    14221 aatcaagttg gatagtagaa agggtttctt cttttccaag ggcagtttta atctggatat
    14281 ccaattttgg tccgtagaag gctgcttcac cttcggcttc aaagtagtcc acgcccattt
    14341 catcaagagc tgcacgaagc atggtttggg cattttccca catctcatcg ttatcaaagt
    14401 acttatgagt atcttgaggg tcacgaagag agaggcggaa gcggtagtca gtcaagttga
    14461 agtcttcata aacatcgata atcaactgaa gggcacgttg gaattcttct tggatttgtt
    14521 ctggagtaac gaataggtga ccgtcgttga gtgacatttc acgtacacgt tgaaggccag
    14581 tgagggcacc agatttttcg taacggtgca tcataccgat ttcagcgata cggattggca
    14641 attcacggta agagtgaaca tggtgtttga aaacttggat gtggtgcgga cagttcattg
    14701 gacgaaggac aaattcttcc ccgtcaccca tgtccatggt tgggaacatg tcttcttggt
    14761 aatgatccca gtgaccagaa gtcttgtaaa gctcaacaga agcaagtggt ggagtgtaga
    14821 cgtgttggta gccagaagcc aactctttgt ttacgatgta gcgttccaat tcacgacgga
    14881 tagtcgcacc atttggcaac cagaatggca aaccttgtcc cacttcttgt gaaatcataa
    14941 agaggtcaag ctctttacca agtttacggt ggtcacgttc cttagcttct tcacgcattt
    15001 gaaggtagtt tttcaagtct ttcttgtcaa accaagctgt accgtagata cgttgcatca
    15061 tagcgttgtc gctgtttcca cgccagtacg caccagctac atggagaagg tggaagattt
    15121 ggatacgacc tgttgatgga acgtgaggtc cacggcagag gtctacatat tcaccctgac
    15181 gatagatagt caaaccgcct tcgtcttctg agtgttcttc aatcaattcc aacttgtaag
    15241 ggtcattttt gaagatttca cgtgcctcgt ctttagtcac ttcttcacga atagatggga
    15301 agttttcttt gacgattttt tgcatttctt cttcgatacg aggaaggtct tcgttagaga
    15361 tttgaccagc tgtgttgtca gtatcgtagt agaaaccatc ttcgatggct ggaccaactc
    15421 ccaagtgaat gtctgggaaa agacgacgag ctgcttgggc gaacaagtga gctgctgagt
    15481 gacgcaagat tggaagggca tcttcgtgat caggtgtcac aatttcgatg cttccatctt
    15541 cagtgatagc gcgagtagtg tcgatgagtt tgccgttgaa tttaccagcc aaggcttttt
    15601 tagctaggga attgctgata gattgggcaa tttcaaaagt tgttacgcca gattcgaatt
    15661 cacgaatagc gccatctggg aaagtaatgt taatcatgat gttctccttt tttattattt
    15721 catagtttct aagcattgat tttttatcag cagactactt ccaagtccta acgttcaatt
    15781 gcttttcttt atcagagagg aagttttcca tttccgtcag aatatctata tccaaacgtt
    15841 gagaaagttc taatagtcac cagatatttt ctgaaagttt ttgttccagt gtgtagggtg
    15901 tttcatcata gtagcgtcct tgttttgtca tcaccagtcg ttggaaattt ccaatatcat
    15961 tagataaagc caagaggtct tcttctaccg tccacttgga atcatgatgc ttaacttcca
    16021 gttcgtgata agcttgtcgg attgcccaac tgcgctccac taattcttgc aactccataa
    16081 ttagtatcct aataaaaaaa tgcgacatcc tcgaaaggac gtcgcaacgt ggttccacct
    16141 tcatttatgt acctcaaaag agagggacac ctctgattgg ctctaacgtg gccaccgttt
    16201 tatgttttca taaaaaactc aagagtagta tcagtttaga cttcctatgc gtttccagca
    16261 accacgcact ctctagtaga aagggactaa gtgacttgtc tctatggatt attatagcat
    16321 gattgtgaga tttttcaagg attttgtgaa agttttttgt ttttcatttt ttgtagaata
    16381 tgcttgatgc taataccaga atccatgccc accagagcaa ctgtttcata gattagcaag
    16441 taaattagta taaactcacc aaaggttatg atagtaaagt aaaagagcag tccgttacca
    16501 actaaccatc aaagtgagaa acggaaacgg tagctataga gtaacgatac aatcaagatt
    16561 accagaggtg taaagaagag aagattgttt atatagtgta agggggtcag aaacaactag
    16621 caagaacaac tcataaagtg gccaataaaa gaataatatg accagatagt aggggagatg
    16681 ggaatagaat ctacgcatga taatcacctc gttttaaaaa aacagacaac ttagatctct
    16741 tgctatgatt attatagcat gtttaaaaca agaaagtaaa tttgttgaca aagaagattt
    16801 ttcttttcac tagaatcatg gttagttttc ttggtagaaa tattttgata ataattaagc
    16861 aagactattg acattgatta ttaagagtgt cacaatttct gtaactaaaa taattacaac
    16921 aagagaggta taaaaaatga aactagcagt tattgcagca aatggacaag cggggaaagc
    16981 aattgttgaa gaagcagtca aacgaggaca tgaagtaacg gctattgttc gttctgaaaa
    17041 caaaagtcaa gctgagtcta ttattaaaaa agatttattt gagttgacaa aagatgatct
    17101 tactggattt gatgctgtta tttcagcgtt tggtgcttat acaccagata cgctaccgct
    17161 ccacagcaaa tcaatcgaac tatttaatca gcttcttgct ggaacacaga cacgttttct
    17221 agttgtaggg ggagcaggaa gtctttatat agatgaaaca aaaacgacac gacttttaga
    17281 tacgtctgat tttcctgaag aatttaagtc actagcaaaa gctcaagcgg atgaattgga
    17341 tcttcttcgt acaaaaaata atctcaactg gacttttgtc agtcctgctg tagattttat
    17401 tcccgatggt gaaaaaacag gaaactaaat tctagcagga gaaattttta ctactaatgg
    17461 aaaaggaata agccagatta gttatgcaga ttatgctatt ggattggtag atgaacttga
    17521 aaaaggtcat catataaaag aacgtatctc tttacttgaa aaataataac tatgagagta
    17581 ttaatgtttg acagaaaatt taaatcttgg taaaataggg attgtcgtaa agacaaataa
    17641 cttcttcttg gttacaggca tgccaacctg tcactcggat gaagccaaat aaaaaggaga
    17701 aacatcatgg caatctcaaa agagaaaaaa aatgaaatca ttgcacaata tgcacgtcac
    17761 gaaggtgata caggttcagt agaggttcaa gttgctgtcc ttacttggga aatcaatcac
    17821 cttaacgaac acatcaaaca acataaaaaa gaccacgcta cttaccgtgg attgatgaaa
    17881 aaaatcggtc gccgtcgtaa cttgcttgca tacttgcgta aaaacgacgt taaccgttac
    17941 cgtgagttga tcaactctct aggacttcgt cgctaattca agatacaaag gccgtcaaaa
    18001 gcacaaagca aaaataggaa aattgacgaa gaaacttcag tttctaggag attttatctt
    18061 ttttgccaag tgcttaggcc gtgttcaatt gagcatatct tgataataaa gctactctaa
    18121 attgggtagc ttttttgcgt tgtcacctta cctcgatata ctcaagtata atctttggtt
    18181 acggttccta gcactgtaag gtaaaataaa ccagcttttc tcccttcggg gagatttttt
    18241 ttgtttcact gaaagtttcg tacgatcttc gcctcgtcgc ctagtctata aacgatttat
    18301 ctagcaagaa tcatgatgct aagggcgtca aaaatccgta tgaaaatagg gaaatgacac
    18361 agtgttcgat gagcacaagg agtttcatct ttttcactag gattttagcc cgggctcaaa
    18421 tcagctctct gacttcagag ggctttttta ttgaggtttt atcggtaaca attttggaga
    18481 ctacaaaaac ctcaaacggt atcagctaat tacaccataa aggtgcgcag ttactcggct
    18541 tttcaagccg agtagctgta tgcaagcccc tagaagagcc cactctttac aaagtaaagt
    18601 atagtatgtt atactttaca tggaagtagt cactgaattc cagttagaaa ttactttgta
    18661 actacgtttt gaggaggagt aaaatgcttt cctacgttcg acattaccca ctagcgatag
    18721 ctaaattaat gtgtctgtgc tctcctaaaa tctgctgatt tattactgac taatacagga
    18781 gtttttttat gggacagaca atcatatctg ctattggtgt ttatatttcc accagtatcg
    18841 attatttaat tattttaatt attttatttg cacagctatc acagaataaa cagaaatggc
    18901 atatttatgc ggggcaatat ctaggcacag gcttacttgt aggggcgagt ttagttgctg
    18961 cttatgtcgt taatttcgtg cctgaagaat ggatggttgg attgcttggt ttaatcccta
    19021 tctatttagg gattcgcttt gcaattgttg gagaagatgc ggaagaagaa gaggaagaaa
    19081 ttattgaaag attagaacaa agcaaggcaa atcaactgtt ttggacagtt acattgctga
    19141 caattgcgtc tggcggagat aatttaggta tctatatacc ttattttgct tcgttagatt
    19201 ggtcacagac cctcgtggcc ttgcttgtgt ttgtaatcgg cataattatc ttttgcgaga
    19261 ttagtcgggt gttatcctct attccgttaa tattcgagac aattgaaaaa tacgagcgaa
    19321 tcattgtgcc cttagtattc attctacttg gactatacat catgtatgaa aatggcacga
    19381 tagagacttt tctgatcgtg tagatttttt tgtttcacta ggattttagc ccgagctcaa
    19441 atcagctctc tgattttcag agggcttttt cattgagtat aaatggacaa tcttaaagtt
    19501 actctattgt agagtggctt ttgatttgat taggagattc ttataaatat agtaaaatga
    19561 aacaaaaaca ggacaaatcg atcaggacag tcaaatcgat ttctaaaaat gttttaagaa
    19621 gtagaggtgt actattctag tttcaatttg atatataaat gaaaacctta gctgtttggc
    19681 caaggttcat ttcttctatg caagttcttc tcttttctta tctgggttcc agatgaagct
    19741 tgcgataaag ctaaagatga tggttaggat agcgaggata atagcaagga tgagggcagg
    19801 gatgcggata aaccaccaga gtggatttgg ttttttatca ttgtgccatt gtttcgtttc
    19861 ttcgtccaga cgttggaatt ctgtttgaat acgattggcg actgtttcaa tgccttcatc
    19921 attcattttc ttgatatctg agatatcgat tggatttcca aagttcatat cgacacgttc
    19981 acggctaatc aagcccttca aagtcatggg accggtgtag gtaaccggca tgatacggac
    20041 cttggccatt ttggcaatca gtgctgcgcc ccccttgaca tcgtttgagt ggcggctacc
    20101 acttggaaac atgatgagag agcggtcact ttttttgaga acgttgatag gatatttgat
    20161 ggctgaggcg ctgggatttt cacggtcgat gggaaaggcg ccacacatac gaacccacca
    20221 accaaagata cggttggtaa agagttcttt ttttgccata aagatgaact gttttggctt
    20281 ggtcgcaaag gccatataaa caggatccca ccaggtacgg tgaggcgcaa ctaaaatata
    20341 attttcatct tgattaggaa ttttatcagt attatgatag tgagcattgc cattgatgga
    20401 ccataagagc aatacaacta atccacgcaa ataagtataa aacatgcgat ctccttcgat
    20461 tgttttcttg ttattattat accttatcaa aggagggctg gcaaactttt cccttgacta
    20521 gatgcatatt tgggatgaaa ttagaattct gttaaaaaaa atgatataat agaatttatg
    20581 gataaaaata agattatggg attaacccaa agagaagtca aggaaagaca ggctgagggt
    20641 ttggtcaatg actttaccgc atcagccagt accagcactt ggcaaatcgt taaacgaaat
    20701 gtctttaccc tttttaacgc tttgaacttt gccattgctt tggcccttgc ctttgtgcag
    20761 gcttggagca atctggtctt ctttgctgtt atctgcttta acgctttttc tgggattgtg
    20821 accgagctac gagccaaaca catggtggac aagctcaatc tcatgaccaa ggaaaaggtc
    20881 aaaaccatcc gtgatggtca ggaagttgct cttaatcctg aagaattagt gctaggagat
    20941 gtcattcgtt tgtctgcagg agagcagatt cctagtgatg ccttggtttt ggaaggcttt
    21001 gcggaagtca atgaagccat gttaacggga gaaagtgatt tggtgcaaaa ggaagttgac
    21061 ggcttacttt tgtcaggaag tttcctagcc agtgggtcag ttttatctca agttcaccat
    21121 gtcggtgcag acaactatgc tgccaaactc atgcttgagg ctaagaccgt taaacccatc
    21181 aactcccgta tcatgaaatc gctggacaag ttggctggtt ttactgggaa gattatcatt
    21241 ccctttggtc tggctctctt gctggaagcc ttgcttttaa aaggcctgcc tctcaagtca
    21301 tccgttgtaa actcgtcgac agctcttttg ggaatgttgc ctaagggaat tgcccttttg
    21361 accattactt cgctcttgac tgcagtgatt aagttgggct tgaaaaaggt cttggtgcag
    21421 gagatgtact ctgttgagac cttggcgcgc gtggatatgc tctgtctgga caagacgggc
    21481 accatcaccc aaggaaagat gcaggtggag gctgttcttc cactgacgga aacttacggt
    21541 gaagaggcta ttgccagcat cttgactagc tacatggccc atagtgagga taagaatcca
    21601 actgcccaag ccattcgcca gcgttttgtg ggagatgttg cttatcctat gatttccaat
    21661 cttcccttct ccagcgaccg caagtggggg gctatggagt tagaaggctt ggggacagtt
    21721 ttcttagggg cacctgagat gttgctggat tctgaagtcc cagaagccag ggaggccttg
    21781 gagagaggat cacgtgtctt ggtcttagct ctcagtcagg agaaattaga ccatcacaaa
    21841 ccacagaaac catctgatgt tcaggctcta gccttgctgg aaatcttgga ccccattcga
    21901 gagggagcag cagagacgct ggactatctc cgttctcagg aggtgggact caagattatc
    21961 tctggtgaca atccagttac ggtgtccagc attgcccaga aggctggttt tgcggactat
    22021 cacagctatg tagattgctc aaaaatcacc gatgaggaat tgatggccat ggcggaggag
    22081 acagctattt tcggacgtgt ttcccctcat caaaagaaac tcatcatcca aacgttgaaa
    22141 aaagcgggtc atacaacggc tatgacaggg gacggggtta atgatatctt ggcccttcgt
    22201 gaggcggatt gttctatcgt gatggcggag ggagatccag caacccgtca gattgccaat
    22261 ctggttctct tgaactcaga ctttaatgat gttcctgaga ttctcttcga gggtcgtcgc
    22321 gtggtcaata acattgccca catcgccccg attttcttga taaagaccat ctattccttc
    22381 ctgttagcag tcatctgtat tgccagtgct ttactaggtc ggtcagagtg gattttgatt
    22441 ttccccttca ttccgatcca gattaccatg attgaccagt ttgtggaagg tttcccacca
    22501 ttcgttctga cttttgagcg aaatatcaaa cctgttgagc agaatttcct cagaaaatcc
    22561 atgcttcgtg ccctaccaag cgctctcatg gtcgtgttta gcgttctttt tgtgaaaatc
    22621 tttggaacga gtcaaggttg gtctgagtta gaaatctcaa ctctcctcta ttatctcttg
    22681 gggtcaattg gtttcttatc cgtatttaga gcctgcatgc cattcactct atggcgagtc
    22741 ctcttgattg tctggtcagt aggaggcttc ctagccacag ctctcttccc aagaatccaa
    22801 aaactacttg aaatttcaac cttgacagaa caaacgttgc ctgtttatgg tgtcatgatg
    22861 ttggtcttta cagtgatttt catcctgact agtcgctatc aagctagaaa ataaagaaag
    22921 actgcaatct gtggattgca gtttttttag gtacaagatt gccagccgaa atgtggtata
    22981 ataagaggta atagagtttt ggaaagtgag agaagatgat ttcaaagaga ttagaattgg
    23041 tagcttcctt tgtgtcacag ggggctattt tactagatgt gggaagtgac catgcttatc
    23101 tgcctatcga gttggttgag agaggccaaa tcaaaagcgc tattgcaggt gaggtggtgg
    23161 aaggtcccta tcagtctgcg gttaaaaatg ttgaggctca cggcctaaag gagaaaatcc
    23221 aagtccgttt agccaatggc ttggcagctt ttgaagagac tgaccaagtg tctgtcatta
    23281 ccattgctgg catgggtggt cgtttgattg ctaggatttt agaagaaggt ttggggaagt
    23341 tagctaatgt agaacgtttg atcctccagc ccaataatcg tgaagacgac ttgcgtatct
    23401 ggctacagga tcatggattc cagattgtag cagaaagcat cttagaagaa gctggaaagt
    23461 tttatgagat tttggtggtg gaagcaggac aaatgaagct atcagccagt gatgttcgct
    23521 ttggtccctt cttgtccaaa gaagtcagtc cagtatttgt ccaaaaatgg caaaaagaag
    23581 ctgagaagct agagttcgcc ctcggacaaa tcccagaaaa aaatctggaa gaacgtcaag
    23641 ttctagtaga taagattcaa gctatcaagg aggtgctcca tgttagcaag tgaagtgatc
    23701 caagcttatg aagccttttg ccctcaggaa ttttctatgg agggagacag ctgtggtctg
    23761 caaattggca ctttagacaa gggtatccaa agggtcatgg ttgccctcga tattcgtgaa
    23821 gagacggtgg ctgaagccat tgaaaagggt gtagacttga ttatcgtcaa gcacgcgcct
    23881 atctttcgtc ctatcaagga cttgcttgcc agccgtccgc aaaatcagat ttacatcgac
    23941 ctgattaagc atgatatcgc agtttatgtc agccatacca atattgatat cgttgaaaat
    24001 gggctcaatg actggttttg tcagatgcta ggaatcgagg agacgactta tctgcaggaa
    24061 acaggtccag aacgtggaat tggacgtatt gggaatattc agcctcagac attttgggaa
    24121 ttggcccaac aggtcaagca agtctttgac ctagatagcc ttcgaatggt gcattatcaa
    24181 gagaatgatt tgcagaagcc tatttcaaga gtagcaattt gtggtggaag cgggcagtct
    24241 ttctataagg atgctttggc aaagggggca gatgtctata tcactggtga catctactac
    24301 catactgctc aggatatgct gtctgatggc ttgttagcat tggatccagg tcactatatc
    24361 gaagtgcttt ttgtggaaaa aatcgcaaca ctccttactc aatggaaaga gaaaaaaggc
    24421 tgggaactag agattttacc tagtcaagca tcgaccaatc ctttccgtca tatctagtta
    24481 gaaagtgaag acaatgaaaa aagttgccat tattggagca ggaattgtgg gagcaacagc
    24541 tgcctactac ctctcgagag aaagtgacct agaggtgacc gtttttgacc atgagcaagg
    24601 tcaagccacc aaggccgcag caggaattat cagtccttgg ttttccaaac gccgtaataa
    24661 agcctggtac aagatggcgc gcttgggggc tgacttttat gtggatttat tagctgattt
    24721 agagaaatca ggacaaaaaa tcgactttta ccagcgttcg ggagtctttc tcttgaaaaa
    24781 ggatgaatcc aagttggaag aactttatca actggccctc cagcgcagag aagaatctcc
    24841 cttgataggg caattagcca ttctggacca agcttcagct aatgaattat tccctggttt
    24901 gcagggattt gaccgcctgc tctatgcttc tggtggagcg agagtagatg gccaactctt
    24961 ggtaactcgt ctgctagaag ccagtcatgt caagctggtc aaagaaaaag tgactctgac
    25021 actcttatca tcaggctacc agattggcga agaggtgttt gatcaggtta ttttggcaac
    25081 gggagcttgg ttgggggaca tgttagagcc tttaggttat gaagtggatg tccgtcctca
    25141 aaaaggacaa ctacgagatt atcagcttgc ccaagacatg gaagattacc ctgttgtcat
    25201 gccagaaggg gagtgggatt tgattccctt tgcaggtggg aaattatcct tgggcgctac
    25261 ccatgaaaat gacatgggat ttgatttgac ggtagatgaa cccttgctcc aacaaatgga
    25321 ggaggcggcc ttgcctcatt acccagtttt agctagagcg acttcaaggg ctgagcgtgt
    25381 gggaatccgt gcctatacca gtgatttctc tcccttcttt gggcaggtgc cagaattggc
    25441 aggtgtctat gtagccagtg gactaggttc atcaggcctc acaactggtc ctatcattgg
    25501 ttaccatcta gcccaactga tccaagacaa ggagttgacc ttggaccctc taaattaccc
    25561 aattgaaaac tatgtcaaac gagtaaaaag cgaataatac tcaatgaaaa tcaaagaaca
    25621 aactaggaaa ctagccgcgg attactcaaa gtactgcttt gaggttgtgg atagaactga
    25681 cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag tcagctcaaa
    25741 acactgtttt gaggttgtag atagaactga cgaagtcagt aaccataccc atggcaaggc
    25801 gacgttgacg tggtttgaat tggattttcg aagagtataa gcattttact gaaattttat
    25861 caggtagttt aggatggcaa atgacattcc ctgtcaaaaa tggtaaaata agaaaaaata
    25921 atctgagaat cgaggaaaaa agatgcaaga aaagattttg gtaactggtg gtgccggttt
    25981 tattggaacc cacactgtta ttgaattgat ccaagcaggc catcaagttg ttgtggtgga
    26041 taatcttgtc aatagcaatc gtaagagttt agaagttgtt gaaagaatca caggagttga
    26101 gattcctttc tatgaggcag atattcgtga cacagatacc ctcagagata ttttcaagca
    26161 ggaagaaccg actggtatca ttcactttgc tggtttgaag gctgttggcg aatcaacacg
    26221 tatccctctt gcctactatg acaacaatat cgctggaact gtcagccttt tgaaagccat
    26281 ggaagaaaac aactgtaaaa acatcatctt cagttcttct gcgacagttt acggggatcc
    26341 gcacacagtg cccatcttgg aagatttccc actttcagtg accaacccat acggtcgtac
    26401 taagctcatg ctagaggaaa ttttgaccga tatttacaaa gcagactcag aatggaatgt
    26461 tgtcttgctt cgttacttta acccaatcgg agcccatgag agtggtgatt tgggagaaaa
    26521 tccaaacggt attccaaaca atctcttgcc atatgtgact caagtagccg ttggaaaatt
    26581 agagcaagtg caagtgtttg gagacgatta cgatacggaa gatggaacag gtgttcgtga
    26641 ctatatccac gttgtcgatt tggctaaggg tcacgttgca gctttgaaaa aaatccaaaa
    26701 aggttcagga ctaaacgttt ataaccttgg aactggtaaa ggttactcag ttcttgaaat
    26761 tatccaaaac atggaaaaag cggtgggacg tcctattcct taccgcatcg tagaacgtcg
    26821 cccaggtgat atcgctgcct gctactcaga cccagcaaaa gctaaagcag aactcggttg
    26881 ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca tggcgttggc aaagcaagca
    26941 tccaaatgga tttgaagact aagatgatga tttcaatcat cgtcccttgt ttaaacgaag
    27001 aggaagtact tcctcttttt tatcaggctc tggaagcttt acttccagat ttggaaacag
    27061 aaatcgagta tgtctttgtc gatgatggat caagtgatgg gaccttggaa ctcttaaagg
    27121 cctatcggga gcaaaatccg gcagtccatt atatttcttt ctctcgaaat tttggcaaag
    27181 aagcagccct ttatgcaggc ttgcaatatg cgacaggaga tttggtggtg gtgatggatg
    27241 cagacctcca agatcctcct agtatgttgt ttgagatgaa aaatgtacta gacaaaaatg
    27301 tagacttgga ctgcgttggg acacggagaa ctagtcggga gggagaaccc ttctttcgca
    27361 gtttctgtgc tgttctcttt tatcgcctca tgcaaaaaat cagcccagta gctctgccgt
    27421 cgggtgtccg tgattttcgt atgatgagaa ggtctgtagt cgatgccatt ttaagcttga
    27481 ccgagtccaa tcgtttttca aaggggctct tcgcctgggt gggtttcaaa acctactatt
    27541 tggactatcc aaatgtcgaa aggcaggctg gcaagacaag ttggagtttt aggcaactct
    27601 ttttctactc cattgaaggg attattaatt tttcagattt tcccttgatt atagcctttg
    27661 tagctggtct cctatcttgt tttctttctc tgctgatgac cttttttgtt gtggttcgga
    27721 ccctcatttt gggcaatccg acatctggtt ggacctctct gatggctgtt attctctttc
    27781 ttggaggcat tcaactcttg accattggga ttctcggcaa gtatatcagt aagatttatt
    27841 tagagactaa aaaaagacca ctttatctta tcaaagaaaa aagtgacctt cctgatttta
    27901 cagaaaaaaa taaagtgaaa agactataat tttacatgga aatgtgctaa actagaggga
    27961 gtggattatc gccattgatt taggcttcct ggtttccaat cagggcgcga gttgggcaat
    28021 ttttaacagc ctctaagaca tcctgactag gagaaatttc tttttccagt tggtcagggt
    28081 catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa taagtttggc
    28141 aaagcccaca ggcgatacat cgttcaggta taagtgtgat tttcatgttc atatttatat
    28201 tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac cgtggcaaga
    28261 agatatctat gatcaagaag aatcaagagc agagcgtcgg catcgaaacc acggaggggc
    28321 tgataggatg gctaatcgta ttttgacgat cctagctagt attttctttg taattgtggt
    28381 ggtgatggtc atcgttctca tctatctatc atcggggggg agtaatcgca cagcagcctt
    28441 aaaagacttt catgattctg atgcaagtgt agtacaaatc tcatcttcaa gcagttctca
    28501 gcctgagcag agttcagagc cagaatctac ttctagtagt tcagaagaag ctgctaatcc
    28561 tgaaggaacg attaaagttc tcgcaggaga aggggaagca gctattgccg ctcgtgcagg
    28621 aatctccatt gctcagttag aggccttgaa tcctgggcac atggctacag gatcttggtt
    28681 tgctaatcca ggtgatgtta taaaaataaa ataggagccg atatgaaaac aattcaaatt
    28741 gctattgatg gtcctgcttc cagcggtaag agtacggtcg caaagattat tgctaaggat
    28801 tttggattca cctaccttga tacaggagct atgtatcgtg cagcgaccta tatggctctt
    28861 aagaaccaat tagtagttga agaagtcgaa gcccttctag ccttgttgga ccagcatcca
    28921 atcagctttg gacgttcaga aactggagac cagcttgttt ttgtaggaga tgtggatatt
    28981 acccatccta tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc agcaattcct
    29041 gaagtgcgtg agaaactggt ttctctccaa caagaaattg cccagcaagg cggaattgtc
    29101 atggatggtc gcgatattgg aactgttgta ttgccacaag cagaattgaa aattttccta
    29161 gtagcttctg ttgatgagag agcagagcgt cgttacaagg aaaatattgc caagggaatt
    29221 gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa ggatagtcat
    29281 cgtgagactt ctcctctcaa acaagcagag gatgctgtct accttgatac aactggtttg
    29341 aacattcaag aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat gtaaatgtag
    29401 aaaagccgat agattgatat cggttttttc taaatttgtc aaaatgctga ttttagggta
    29461 taatagttgc tgttcgagaa attttgattt tcaaagaata gtgaatgatg ataaggagaa
    29521 atcatgaaca atttaccaaa ttgtccaaaa tgtaactcag agtatgtcta cgaagacggt
    29581 gccctactgg tttgcccaga gtgtgctcat gagtggaatc ctgctgaagt tgcagaagta
    29641 gaagaaggtc ttgtcgctat cgatgccaac ggaaataaat tggctgatgg tgatacagta
    29701 actctcatca aggacttgaa agtaaaaggt gcgccaaaag atttgaaaca agggacgcgc
    29761 gtgaaaaata tccgcatcgt agaaggcgac cacaatatcg actgtaagat tgatggtttt
    29821 ggtgccatga aacttaaatc agagtttgtg aggaagattt aagcatgtca aaacactata
    29881 aacttgtatt ttatagccgt atcttcttgt ttctagcggc ttttacggga gtttatcttg
    29941 aaattactaa gcatggtggt tttgggatgc ttctctatta cacggttctg tccaacctct
    30001 tggtaactat ttttaccctt tatcttctaa aggttatgag ccgtgtaggt gaaaattggc
    30061 aaagaccaag tctcttgcgc ttaaaaggtg gggtcaccat gagtatcatg attacctgtg
    30121 tgatttacca tttcctcttg gcgcccattg cgactaattt ctatacccta gaaaatttcc
    30181 tttgccacta tatcgttccc atctggtttt tagcggatac cctctttttt gacaaacagg
    30241 gtcaatacaa gatttgggat ccagcagtgt ggaccatttt accctttctg tatatgatgt
    30301 ttgctctttt taatggcttg gttctaaaac tcaatattcc aaatgccaag gataatcctt
    30361 tcccttactt ctttttgaat gtgaacaagg gttggaatgt tgtgtttaag tggtgtctga
    30421 ttatctttgt tgcctatatg gtagcaggat ttattttcta ctttatcaag caaatcaaga
    30481 gaaagtcatc ctaatactct tcgaaaatct cttcaaacca cgtcagtttc catctgcaac
    30541 ctcaaaacac tgttttgaac aacctgcggc tagcttccta gtttgctctt tgattttcat
    30601 tgagtataag atacaaggga ttcattttcg aatttaagaa ggagtctaca aaccaatgag
    30661 gcttcttctt ttcttgctta tttgataact atcaaaaaag tagaagatta agagaaatat
    30721 agaaagagat ttcatgaaac aatttttaga acgggccagc attttggctc tctccctcgt
    30781 tttgattacc tccttttcca tttcgagtgc tctaccagcc atgtttgact attatcaggg
    30841 ttattctaag gaacaaattg agctcttggt gagcttgcct tcctttggaa tcatgatgat
    30901 gttactgcta aatggtttct tagaaaaaat atttcctgag cgcttacaga ttagtttggg
    30961 cttgctgatt ttatcattga gcggtacagc tcccttctgg taccaagcct atccctttgt
    31021 ctttggaaca cggcttctct ttggtttggg tcttgggatg atcaatgcca aggccatttc
    31081 tattatcagt gaacgctacc aaggaaaaag gcgaattcag atgttagggc tacgcgcttc
    31141 tgcagaggtc gttggagctt ctctcattac cttggccgtc ggtcagttgt tggcctttgg
    31201 ttggacagct atctttctag cctatagtgc tggatttttg gtgctgcccc tttatctgct
    31261 ctttgtccct tatggaaaat caaagaaaga agtcaagaaa agagcgaagg aagcaagtcg
    31321 tttaactcga gaaatgaaag gcttgatttt taccttagct atcgaagcgg cagttgtagt
    31381 ttgtaccaat acagctatta ccatccgtat tccaagtttg atggtggaaa gaggattggg
    31441 ggatgcccag ttatctagtt ttgttcttag tatcatgcag ttgatcggga ttgtggctgg
    31501 ggtgagtttt tctttcttga tttctatctt taaagagaaa ctgctcctct ggtctggtat
    31561 tacctttggc ttggggcaaa ttgtgattgc cttgtcttca tccttgtggg tggtagtagc
    31621 aggaagtgtt ctggctggat ttgcctatag tgtagtcttg acgacggtct ttcaacttgt
    31681 ctctgaacga attccagcta aactcctcaa tcaagcaact tcatttgctg tattaggctg
    31741 tagtttcgga gcctttacga ccccatttgt tctaggtgca attggcttac taactcacaa
    31801 tgggatgttg gtctttagta tcttaggagg ttggttgatt gtaatctcta tctttgtcat
    31861 gtacctactt cagaagagag cttaggattg attcctaagt ttttcttttg atactcaatg
    31921 aaaatcaaag agcatagttg attgagtttg gaatagtacg ctgtagttgc taaaatattt
    31981 ctagaaatta atttgacttt tctaatagag ttattcatat cttatttcaa tctactatag
    32041 aaagctagcc gcaggttgct caaaatagtg ttttgaggtt gcagatggaa actgacgtgg
    32101 tttgaagaga ttttcgaaga gtatgagatt tttataccca tacaatattt gttttaggtc
    32161 cttgtttaca gactaatttc ttgataaaat agaggttatg acgagatata aagcaactat
    32221 ttcctatgat ggttatgcct ttgctggctt tcagcgccag tctcatgcgc gtagcgttca
    32281 ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg caaaccatta ctgttcacgg
    32341 tgctggtagg acagatagtg gggttcatgc cctgggacag gtcattcatt ttgacctgcc
    32401 ttatcagatg gatgaggaga aactccgttt tgccttggat acccagtctc ctgaagatat
    32461 tgatgtgatt tcgattgagc ttgtggcaga tgattttcat tgccgttatg ccaaacatag
    32521 caagacctat gagtttattt tggatagagg acgtcccaaa aatcctatgc gccgtcacta
    32581 tgccacccac tttccctacc cactcgatgt ggaacgaatg cagattgcaa tcaaaaagct
    32641 agagggaacc catgatttta ccggttttac agcctctggg actagtgtag aggataaggt
    32701 tcgcaccatc acagaagcta gtttaatagt cgatgagaca ggacaatttt tgacctttac
    32761 cttttcagga aatggtttct tgtataaaca gattcgcaat atggtgggga ctcttctcaa
    32821 aatcggtaac aaccgcatgc cagtagagca gattgatctc atcttggaga agaaggacag
    32881 gcaacttgca ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga ttcgttatga
    32941 agaataatcg tattttagca ctttctggaa atgatatttt tagtggtggt ggactgtcag
    33001 ctgatttggc tacctatacc ttgaacggct tgcatgggtt tgtagcagtg acttgtttga
    33061 cagccttgac agaaaaagga tttgaagtct ttccaactga tgataccatt tttcaacatg
    33121 aattagatag cttgcgtgat gtggaatttg ggggaattaa gattggtctt ctccctactg
    33181 tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg cccaggagta cctgtggtgt
    33241 tggatcctgt cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag ctctgccaag
    33301 agttgattcg ctttttccct tatgtcagtg tgattacgcc taatctccca gaagcagaat
    33361 tattatccgg tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg cagaaattgc
    33421 atgatttagg agcgccagca gtcattatca agggaggcaa tcgtcttagt caggacaagg
    33481 ctgtggatgt cttttatgat ggacagacct ttactatcct agaaaatcca gttatccaag
    33541 gccaaaatgc tggtgcaggt tgtacctttg cctctagcat tgccagtcac ttgattaaag
    33601 gtgataaact tttgccagca gtagaaagct ctaaggcttt cgtttatcgt gctattgcac
    33661 aagcagatca gtatggagta agacaatatg aagcaaacaa aaacaactaa aatcgccctt
    33721 gtatccctat taaccgccct ttctgtggtt ctaggttatt tcttaaaaat cccaacacct
    33781 acaggaattc taactctttt agatgctggt gtcttctttg cggcctttta ctttggtagt
    33841 cgtgaaggag cggtagtcgg aggactagca agtttcttga ttgacctctt atcaggctac
    33901 cctcagtgga tgttctttag cttggtcaac catggcttgc agggattttt cgcaggattt
    33961 aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta ttgccatggt aggaggctac
    34021 gccttgggtt ctactttgat gaatggctgg gcagcagccc tcccagaaat tctaccaaat
    34081 ttcatgcaaa atatggtagg gatgattgta ggatttattc ttagtcaaag tatcaagaag
    34141 attaagtaat actcttcgaa aatctcttca aaccacgtca gtttccatct gcaacctcaa
    34201 aacactgttt tgagcagcct gcggctagct ttctagtctg attttcattg agcataaaga
    34261 ggttgagtaa aaagtctttc aaataagaaa aacgcatagt ataggatgtt gaaatgatga
    34321 actgcccccc aaaagttaga cagaaaaaat ctaacttttg gggtgtttta ttatgaaact
    34381 aacttatgat gataaagttc agatctatga acttagaaaa caaggatata gcttagagaa
    34441 gctttcaaat aaatttggga taaacaattc taatcttagg tacatgatta aattgattga
    34501 tcgttacgga atagagttcg tcaaaaaagg aaaaaatcgt tactattctc ctgatttaaa
    34561 acaagaaatg attcataaag tctgacatga aggctggact aaagacagag tttctcttga
    34621 atacggtctc ccaagtcgta cgatacttct taactggcta gcacaataca gaaaaaaacg
    34681 ggtatactat tgttgagaaa acaagaggga gagtacctaa aa
//
LOCUS       DAVKWS010000019        34576 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKWS010000019 DAVKWS010000000
VERSION     DAVKWS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.019"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            166..465
                     /locus_tag="VW852_000957"
     CDS             166..465
                     /locus_tag="VW852_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0272125.1"
                     /translation="MKTKKHRLLALVLISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWDGHSDKGEAPAGKTSYAWIWTKWGEQVAFYYDYD"
     gene            536..2557
                     /locus_tag="VW852_000958"
     CDS             536..2557
                     /locus_tag="VW852_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0272126.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASFFILSSRDLVIGLLTLFVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2559..3200
                     /locus_tag="VW852_000959"
     CDS             2559..3200
                     /locus_tag="VW852_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272127.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKSSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3304..4656
                     /locus_tag="VW852_000960"
     CDS             3304..4656
                     /locus_tag="VW852_000960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV0272128.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVSG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4660..5718
                     /locus_tag="VW852_000961"
     CDS             4660..5718
                     /locus_tag="VW852_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV0272129.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5728..6927
                     /gene="divIB"
                     /locus_tag="VW852_000962"
     CDS             5728..6927
                     /gene="divIB"
                     /locus_tag="VW852_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV0272130.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEVEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            7160..7861
                     /gene="pyrF"
                     /locus_tag="VW852_000963"
     CDS             7160..7861
                     /gene="pyrF"
                     /locus_tag="VW852_000963"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV0272131.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            7895..8527
                     /locus_tag="VW852_000964"
     CDS             7895..8527
                     /locus_tag="VW852_000964"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV0272132.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMNLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            8707..9110
                     /locus_tag="VW852_000965"
                     /pseudo
     CDS             8707..9110
                     /locus_tag="VW852_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9206..9970
                     /locus_tag="VW852_000966"
     CDS             9206..9970
                     /locus_tag="VW852_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272133.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGAYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVTYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            9970..10722
                     /locus_tag="VW852_000967"
     CDS             9970..10722
                     /locus_tag="VW852_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272134.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            10732..11430
                     /locus_tag="VW852_000968"
     CDS             10732..11430
                     /locus_tag="VW852_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272135.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            11427..12074
                     /locus_tag="VW852_000969"
     CDS             11427..12074
                     /locus_tag="VW852_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272136.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(12154..13020)
                     /locus_tag="VW852_000970"
                     /pseudo
     CDS             complement(12154..13020)
                     /locus_tag="VW852_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(13032..13790)
                     /locus_tag="VW852_000971"
                     /pseudo
     CDS             complement(13032..13790)
                     /locus_tag="VW852_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(13791..14078)
                     /locus_tag="VW852_000972"
     CDS             complement(13791..14078)
                     /locus_tag="VW852_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0272137.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILILYFAVCYPISKLSTHLEKHWRN"
     gene            complement(14088..14468)
                     /locus_tag="VW852_000973"
     CDS             complement(14088..14468)
                     /locus_tag="VW852_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV0272138.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            complement(14428..15108)
                     /locus_tag="VW852_000974"
     CDS             complement(14428..15108)
                     /locus_tag="VW852_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0272139.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            15323..15469
                     /locus_tag="VW852_000975"
     CDS             15323..15469
                     /locus_tag="VW852_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV0272140.1"
                     /translation="MSYKTSNAEGHVDFINTYDLETMAQQVIPKAAFGYIASGAEDTF
                     ASFQ"
     gene            15597..17087
                     /gene="lysS"
                     /locus_tag="VW852_000976"
     CDS             15597..17087
                     /gene="lysS"
                     /locus_tag="VW852_000976"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV0272141.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            17711..18847
                     /gene="lctO"
                     /locus_tag="VW852_000977"
     CDS             17711..18847
                     /gene="lctO"
                     /locus_tag="VW852_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV0272142.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKDEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      19101..19189
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19404..20072
                     /locus_tag="VW852_000978"
     CDS             19404..20072
                     /locus_tag="VW852_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV0272143.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(20222..20308)
                     /locus_tag="VW852_000979"
                     /pseudo
     CDS             complement(20222..20308)
                     /locus_tag="VW852_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20399..21181
                     /gene="thiM"
                     /locus_tag="VW852_000980"
     CDS             20399..21181
                     /gene="thiM"
                     /locus_tag="VW852_000980"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0272144.1"
                     /translation="MTSLKLLKKKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            21183..21812
                     /locus_tag="VW852_000981"
     CDS             21183..21812
                     /locus_tag="VW852_000981"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0272145.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      22021..22109
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22371..22931
                     /locus_tag="VW852_000982"
     CDS             22371..22931
                     /locus_tag="VW852_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0272146.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            22932..24317
                     /locus_tag="VW852_000983"
     CDS             22932..24317
                     /locus_tag="VW852_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272147.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNLNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDYMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            24319..24969
                     /locus_tag="VW852_000984"
     CDS             24319..24969
                     /locus_tag="VW852_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV0272148.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            24980..25672
                     /gene="tenA"
                     /locus_tag="VW852_000985"
     CDS             24980..25672
                     /gene="tenA"
                     /locus_tag="VW852_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV0272149.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            25677..26201
                     /gene="thiW"
                     /locus_tag="VW852_000986"
     CDS             25677..26201
                     /gene="thiW"
                     /locus_tag="VW852_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV0272150.1"
                     /translation="MRKHQLQVHKLTILSMMTALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            26201..27004
                     /locus_tag="VW852_000987"
     CDS             26201..27004
                     /locus_tag="VW852_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0272151.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQIAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            26997..27629
                     /locus_tag="VW852_000988"
     CDS             26997..27629
                     /locus_tag="VW852_000988"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0272152.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(27838..28629)
                     /gene="thiD"
                     /locus_tag="VW852_000989"
     CDS             complement(27838..28629)
                     /gene="thiD"
                     /locus_tag="VW852_000989"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0272153.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIGSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(28713..28811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            28995..29390
                     /locus_tag="VW852_000990"
     CDS             28995..29390
                     /locus_tag="VW852_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV0272154.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            29401..29772
                     /locus_tag="VW852_000991"
     CDS             29401..29772
                     /locus_tag="VW852_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV0272155.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            29782..32025
                     /locus_tag="VW852_000992"
     CDS             29782..32025
                     /locus_tag="VW852_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0272156.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFRLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            32232..34007
                     /gene="spxB"
                     /locus_tag="VW852_000993"
     CDS             32232..34007
                     /gene="spxB"
                     /locus_tag="VW852_000993"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV0272157.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            34118..34465
                     /locus_tag="VW852_000994"
     CDS             34118..34465
                     /locus_tag="VW852_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0272158.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT        10330 a         6182 c         7543 g        10521 t
ORIGIN      
        1 gaggtaatat agtaaaatat ctaaaaaatt cgattttttc cttgacaaca aataataaaa
       61 atgttattct tatcacacaa gaaagcggtt tcaataaaag agagaggagg gtttattttg
      121 ttgcattgtt gctcaaggtg tgcttttttt gaaagaaaaa ataaaatgaa aacgaaaaaa
      181 catagattac ttgctctagt tcttatttca agttttacat tattgggagc tgcatcagct
      241 gctgtacaat atccagatgg aggagtatgg acatatggag aaggttcagg aggtggttgg
      301 gctttttcaa attactatca tggtaaaaaa tatcattatt catccatcgt gagcagatgg
      361 gatggtcatt ctgataaagg agaagctcct gctggaaaaa cctcctacgc atggatttgg
      421 actaaatggg gagaacaagt agcattttac tatgactatg actaatattt agttttctta
      481 gataattccc cttgtaagaa attacagggg gaattttgtt tacaaggagt ctactatgaa
      541 gaaactattc atattattat caactttttt tctcagcttc ttccttgctt ggattattgt
      601 cttacgtgcg ccacaatatt tatatgcaag ctatgattcc gtttctttac ttcgtgtcaa
      661 aaaagatact caggaaccga cgcgtgaggt atttgaacag gaattggaga attttgcaaa
      721 ctcagaacag agtttaatag ctagaagaat tgtagagccg agtaaggatg ggacgactca
      781 ctttacttat gcaacttatg gtcagggaac tttaccaaaa gaattccaag aagctagcca
      841 agaaagtcgt gaacgtagtg atccgctaaa tagttatctc cttttgtcag gctccttgac
      901 gaaagaaaag cttgccgata aattaggaga tttgggttat aaagcaagtg ctgaccgaaa
      961 gataccgccc tattttcttg cttttcgaat attactaaat ccccttattt taattagttt
     1021 agcaatattt ggcttatctt tctttgcttt agtgattatc actcggatta aggaaatgag
     1081 agcagcaggt ataaaactct tttctggtca gactctctta tccatcatgg ggcattcttt
     1141 atctactgat atcaaatggc tccttctatc agccctcctt tccttcctag gtgggggagt
     1201 cgttcttttt agtcaaggtt tgttttatcc tatcttgtta gccacctatg gttttgggat
     1261 tagtttctat ctgttgtttt tattggcgat ttcaatttta ctaatgcttc tttatctaat
     1321 gagtttgaat tacaaagcat tagttcccgt tattaagggg agattacccc ttaaacgcct
     1381 gatgatttta accctattgt gtcagttggt tgctgttttt acagtaggat acgctgttaa
     1441 gacaggtttg acgtcttacc aacgattgaa agaacttgaa atttcaaaac aagcatggca
     1501 ggatagagca gactattatc aaatttcttt tggcttaggt gatagaggaa aagatacaga
     1561 aaatcagagc aagtggtatg cctttgccaa ggaagcaatc gaagaagaac aagctcttta
     1621 tgtaaaggat aatctgctcc attttgccaa tccacaagga aaaaatgaac agggagagac
     1681 actggatacc tatagtccag atgctaatac gctctatgtt agtcccagtt atttggacaa
     1741 ggaaaaggtc gtggtagatg ctgagaccaa acagaagtta gcccatctcc aaaaaggtga
     1801 gtttatcctc ttgctcccag aacatttgcg ctctcgagaa gcagaactta agaaagtttt
     1861 tgaagaaaga ttgagttatt atggaaaatc tggtgaggag gcaagtgctc ctttggatta
     1921 tgagatgaaa gcgcacgtta gttatctttc aatgggagaa aagcggtttg tttataataa
     1981 cggtgagaat cccgtatcta ctcagtattt gactgatccg attttagttg tattcacgcc
     2041 gacttctaca ggtgatagtt ttatatcctt atctagttgg tctatcaatg ctggaaaaca
     2101 actctttatc aaaggatatg agagtgggct agaactcttg aagaaagctg gaatttatga
     2161 gcaagtatcc tatcttaaag aaggaagaag tgtttatcta actcgttata atgaagttca
     2221 aactgaaaca gcaactttaa tcttaggagc tattgttggg atagccagtt ccttattact
     2281 cttttattct gtcaatcttc tatatttcga gcaattccgc cgagatatct tgattaaacg
     2341 aatttcaggt ttacgatttt ttgaaacaca tgctcagtat atggttagcc aatttgccag
     2401 ttttgtattt ggtgctagtt tctttatttt aagcagtcga gacttggtga ttggcttgct
     2461 cactttattc gtctttctag ctagtgcagt tttgacgctt taccgtcaag cacagaaaga
     2521 atctcgtgtt tctatgacaa ttatgaaagg aaaataggat gattgaacta aagaatatat
     2581 ctaaaaaatt tggaagccgt cagctatttt cagatacaaa tcttcatttt gaaggtggga
     2641 aaatttatgc cttaatcggt acaagtggct gtggtaagac aacactcttg aatatgattg
     2701 gacgattaga gccatatgac aaagggcaaa tcatctatga tggcacttct cttaaggaca
     2761 tcaagtcttc tgttttcttt agagattact tgggatactt atttcaagat tttggcttaa
     2821 ttgaaagcca aaccgtcaaa gagaatctca atctgggttt agttggtaaa aagttgaagg
     2881 aaaaagagaa aatctctttg atgaaacaag ctctaaaccg tgtaaacctc tcttatttgg
     2941 atttgaagca acctatattt gagttatcag gaggagaagc acaacgtgtt gcattagcga
     3001 agataatttt aaaggatccg cctttgattc ttgcagatga accaaccgct tccttagacc
     3061 ccaaaaactc tgaggaatta ctttccatcc tagaatcttt aaaaaatccg aatcggacca
     3121 ttattattgc gacccacaat cctctgattt gggagcaagt ggatcaggtc attcgagtta
     3181 ccgatttatc acatagatga tatggtaaga ttcagttaga agaaagagtc acaaacacac
     3241 tttgtggctt ttttatttcc ataaaaatgg taaaatagta ggagtagaaa tggagttcga
     3301 gacatgaaag taatagatca atttaaaaat aagaaagttc ttgttttagg tttggccaag
     3361 tctggtgaat ctgcggctcg tttgttggac aagttaggtg ccattgtgac agtaaatgat
     3421 gggaaaccat tcgaggacaa tccagctgcc caatgtttgc tggaagaagg gatcaaggtc
     3481 attacaggtg gccatccttt ggaactcttg gatgaagagt ttgccctcat ggtaaaaaat
     3541 ccaggtatcc cctacagcaa tcccatgatt gaaaaggctt tggccaaggg aattccagtc
     3601 ttgactgagg tggaattggc ttatttgatt tcagaagcac cgattattgg tatcacagga
     3661 tcgaacggta agacaaccac aacgactatg attggggaag ttttgactgc tgctgggcaa
     3721 catggtcttt tatcagggaa tatcggctat cctgccagtc aggttgctca aatagcgaca
     3781 gacaagaaca cgcttgttat ggaactttct tctttccaac tcatgggtgt tcaagaattc
     3841 catcctgaga ttgcggttat tactaacctt atgccaactc atatcgatta tcatggatta
     3901 tttgaggaat atgtagcagc caagtggaat atccagaaca agatgacagc agctgatttc
     3961 cttgtcttga actttaatca agacttggta aaagacttgg cttccaagac agaagccact
     4021 gttgtaccat tttcaacact tgaaaaggtt gatggagctt atctggaaga tggtcaactc
     4081 tacttccgtg gtgaagtagt catggcagcg aatgaaatcg gtgtttcagg tagccacaat
     4141 gtggaaaatg cccttgcgac tattgctgta gccaagcttc gtggtgtgga caatcaaacc
     4201 atcaaggaaa ctctttcagc cttcggtggt gtcaaacacc gtctccagtt tgtggatgac
     4261 atcaagggtg ttaaattcta taacgacagt aaatcaacta atatcttggc tactcaaaaa
     4321 gccttgtcag gatttgacaa cagcaaggtc gtcttgattg caggtggttt ggaccgtggc
     4381 aatgagtttg acgaattggt gccagatatt actggactca agaagatggt catcctgggt
     4441 caatctgcag aacgtgtcaa acgggcagca gacaaggctg gtgtcgctta tgtggaggcg
     4501 acagatattg cagatgcgac ccgcaaggcc tatgagcttg cgactcaagg agatgtggtt
     4561 cttcttagtc ctgccaatgc cagctgggat atgtatgcta actttgaagt acgtggcgac
     4621 ctctttatcg acacagtagc ggagttaaaa gaataaaata tgaaaaaaat tgtctttaca
     4681 ggtgggggga cggttggaca cgttaccctc aatcttttgt taatgcccaa gttcatcgaa
     4741 gatggttggg aagtccacta tatcggggac aagcgtggta tcgaacacca agaaatcctt
     4801 aagtcaggtt tggatgtcac tttccactcc attgcgactg gaaaattgcg tcgctatttc
     4861 tcttggcaaa atatgctgga cgtcttcaaa gtttgctggg gaatcgtcca atcgctcttt
     4921 atcatgttgc gacttcgtcc acagaccctt ttttcaaagg ggggctttgt ctcagtaccg
     4981 cctgttatcg ctgcgcgtgt gtcaggagtg cctgtcttta ttcacgaatc tgacctgtct
     5041 atgggcttgg ccaataaaat cgcctataaa tttgcgacta agatgtattc aacctttgag
     5101 caagcttcga gtttgtctaa ggttgagcat gtgggagcag tgaccaaggt ttcagatcaa
     5161 aaaaatccag aaccagatga attggtggat attcaaagcc actttaatca taaattgccg
     5221 actgtattgt ttgttggcgg ttctgcaggt gctcgtgtct ttaaccaatt ggtgacagac
     5281 cataagaaag aactaacaga gcgctacaat attatcaatc taactggaga ttctagcctg
     5341 aatgagttga gccaaaatct ttttcgtgtt gactatgtga ccgatctcta tcaacccttg
     5401 atggaattgg ctgatattgt tgtgacacga ggtggtgcca atacgatttt tgagctcttg
     5461 gcgatagcaa aattgcatgt cattgtgccg cttggtcgtg aagctagtcg tggtgaccag
     5521 cttgaaaatg cagcttactt tgtaaaaaaa ggctatgcag aagaccttca agaaagcgat
     5581 ttgaccttgg atagtttgga agagaagctt tctcacttac taagtcacaa ggaagattac
     5641 caagctaaga tgaaggcttc taaggaattg aaatctctag cagattttta tcaattgttg
     5701 aaaaaagatt tatcataagg aaagtaaatg tcaaaagata agaaaaatga ggacaaagaa
     5761 atcctcgaag aattgaaaga gttatcagaa tggcagaaac gaaaccaaga atatctaaaa
     5821 aagaaggctg aagaagaggc ggctctagct gaggagaagg aaaaggaaag acaagctcga
     5881 atgggagaag aatctgagaa gtcagaggac aaacaggacc aggagagtga aacagaccag
     5941 gaagattcag aatcagctaa ggaagagtct gaagaaaaag tagcatcctc agaggctgac
     6001 aaagagaaag aagagaaaga agaaccagag tctaaagaga aggaggaaca ggataaaaag
     6061 ctttctaaaa aggctacaaa ggaaaaacca gccaaagcaa agattcctgg tatccatatc
     6121 ttgcgagcat tcacgatttt atttccaagt ctgcttttat tgattgtctc tgcctacttg
     6181 ctcagtcctt atgcgaccat gaaagatatt cgtgttgagg gaacggtgca aactacagct
     6241 gatgatattc gacaggcttc aggcattcag gattcggatt atacgattaa ccttctgcta
     6301 gacaaggcaa aatatgaaaa gcagattaag tctaactatt gggttgaatc agctcaactt
     6361 gtctatcaat ttccaactaa gttcactatt aaggtcaagg aatatgatat tgtggcctac
     6421 tatatttctg gtgaaaatca ttatcctatt ctttccagtg gtcagcttga gactagttct
     6481 gtgagtctga acagtttacc agaaacttat ttatcagttc tctttaatga tagtgaacaa
     6541 atcaaggttt ttgtctcaga acttgctcaa attagcccag aactcaaggc ggctatccaa
     6601 aaggtggaat tagccccaag caaggtgaca tccgatttaa ttcgattgac catgaatgat
     6661 tcggacgaag tcttggttcc tctatctgaa atgagtaaga aactgccata ttacagtaag
     6721 attaagccac aattgtcaga accgagtgtg gtcgacatgg aagctggaat ttacagttac
     6781 actgtggcgg ataaattaat tatggaggtt gaggaaaaag ccaaacaaga ggccaaggaa
     6841 gctgagaaaa aacaagaaga agaacagaaa aaacaagagg aagagagcaa tcgaaaccaa
     6901 acgacccagc gctcatcgcg tcgctaggtt tactttttct cttatagtta tttagtgacc
     6961 atgtttttac ttgacaagtg ctagtagaaa taatagaata atagaaaacc tttaaagcag
     7021 tccagagagg cagctaaggt tagacggtga aagggtggag actacccatt tttcgtggaa
     7081 ccttgctgtt ggcaggttcc ttttttcgtg gcttctgttg gtcagactct ctcactagta
     7141 aaggtaaaag gagaaaccta tgcgagaaca tcgtccaatc attgctcttg attttcctag
     7201 ttttgaggcg gtcaaggaat ttttagctct tttcccagca gaagaaagcc tttatctcaa
     7261 ggtagggatg gagctttatt acgcagcggg gcctgagatt gtgtcctact taaaaggttt
     7321 gggtcatagt gtctttttgg atctcaaact tcatgacatt cctaatacag tcaagtcagc
     7381 catgaaggtc ttgtctcagc ttggtgtcga tatgactaat gtccatgcgg ctggtggtgt
     7441 agagatgatg aaggcggcgc gtgaaggtct tgggagtcaa gccaaattga tcgctgtaac
     7501 tcagctcaca tcaacgtcag aagctcagat gcaggagttt caaaatatcc aaaccagtct
     7561 gcaagagtct gtgattcact atgccaagaa gacagctgaa gctggcttgg atggtgttgt
     7621 ttgctcggct caggaagtac aagtcatcaa gcaggctacc aatccagatt ttatctgtct
     7681 gacaccaggg attcgtccag ctggtgttgc agttggagat caaaaacgag tcatgacacc
     7741 tgctgatgcc tatcaaatcg gcagtgacta tatcgtagtg ggacgtccca ttacccaagc
     7801 tgaggatcct gttgcagctt atcatgccat caaggatgaa tggacacagg actggaatta
     7861 aagaactaga ttagaaaaat aaaaggagaa taccatgaca cttgctaaag atatcgctag
     7921 ccacctcttg aaaatccaag ccgtttacct caaaccagag gaacccttca cttgggcatc
     7981 tggtatcaag tcaccgattt acactgataa tcgtgtgaca ctagcctatc cagaaactcg
     8041 taccctaatt gaaaatggtt ttgtggatgc tatcaaagaa gcctttcctg aagtagaagt
     8101 gattgcagga actgcaacag cagggattcc acacggagcc attattgctg ataagatgaa
     8161 cttgcctttt gcctacatcc gtagcaaacc aaaagaccac ggagctggta atcaaatcga
     8221 aggtcgcgta gctcaaggtc aaaaaatggt agtggttgaa gaccttattt caacgggtgg
     8281 ttcagttctt gaagctgtag cagcagccaa gcgagaagga gcagatgtac ttggagttgt
     8341 agcgattttc agctaccaat tgccaaaagc agataagaac tttgcagatg ctggtgttaa
     8401 acttgtgacg ctttcaaact atagcgagct tatccatcta gcccaagaag aaggttacat
     8461 cacgccagag ggccttgatc ttctaaaacg ctttaaagaa gaccaagaaa attggcaaga
     8521 aggttaggtc agtaagataa agagagacga ggctaccgag tctcttttac cattttattt
     8581 aaaatatgac aatattttaa ataaaataat tgacaacgct ttcttttatt gatataatga
     8641 aaatgtaata tgtatcaaat tgatcaatca actatcttgt ttatcacttc ttaagaggac
     8701 gatgtcatga aaagaagaaa aatatattcc ttttttgcgt agttacaatt gcatctagtt
     8761 tgtttctccc tacagtttta gctagacaga ttggaaatta tgatttaacg ttgccgcgtt
     8821 ggggttcgga tacaactagt gagcttgaga aagaaaacgc ctctgctgga attaataata
     8881 gtgacagcac tggtggcggt aaaaggttaa gtacctctat tcgtagcgcc tatagtgggt
     8941 cagatattac cccggtatat tcattggggt ctggctctag gattgtcatg tactataatg
     9001 gaggtggtga caattatatt ggttctggta ctagattagc tatggcgcca caatttggaa
     9061 atcatgtaag aattcatact tcaggttctt ggagtccaga ttcttattaa cttacttgtc
     9121 agagtaagcc ttaaagatgg ttgattgtgg gtgtagcatg aaaaaagaat gctacaccct
     9181 atttttatta taaggaggag taaggatgga atttttcatt tgtaatcttg tacgagtcgt
     9241 tcaatcacct cgattttata tgtctttatt tttgaccctt ctttgcatga gtttaggaaa
     9301 tttccttgct ttcaatggta tttataaaat tgaaggttta tcgatttttt ttgccgcttc
     9361 ttctattcga ggattttcac cgattagcct agtagctgca cttatctgta cactgcccta
     9421 ttctagtcag ataatagagg atgctgagag tcattttcta acagcacaat tgtgccgaat
     9481 ttctaaaaag aggtatctag ctattgtagg tagtactgca attatttctt cttttctagt
     9541 cttttttctc ccctatttat tattattagg aattaatctt ttagtgactc cttatcagga
     9601 aatttatatt ggagcttata gtggtgcctt aaaagaatta tttgattcta atcagttgct
     9661 ctatagtctt gtgacgactc tttggtatgg agtttggggc gctgtgttct ctatttttgg
     9721 actagctagt gctttgctag tgaagaaaaa aataggagct attttcatcc cagttaccta
     9781 tatgatggtt ggtggtattt tttgggctat tttagggcta tcttacttag aacctgtgac
     9841 aacattagct ttgggatatc agaaagatat cagtctttcc ttagttagtg ggcatcttgc
     9901 ttttatttta tttgttagtt gtttggttgt ttatggtaca ttttttctac attcagagga
     9961 ctatgtataa tgaaacaatt tgttcaattt tataaaaaag atttcttagc agtattggtt
    10021 tattttatat tactgctatc ctgtgtttta tctagtacag tatatttatt gcgctgtcgc
    10081 caatattcaa tccatccaaa tgtattagaa tggatcttag ttttacttca agatatgacg
    10141 actggagtat attgctttcc gttcacatat atattgttct ttttttattt gatgaataac
    10201 tattttaata ggttggagtg tcgcattcgt ctgaaatcaa ttaagcactt taccagtttt
    10261 agtttcaaat tagcagctct tagtacgggg atttggacgg cgattttatt tttattgatt
    10321 tttctaattg catttagtaa tggttttagc ttctctttgg agataaagga ggttgatttt
    10381 ttaagagaat tttatggtat aagtattgca aacaatgcta gtttctttat aggatttttt
    10441 ttctcttata tagcatacta tttcttttta tccttactta ctattagcag tttttcttgg
    10501 tttaaaaaat caaacatgag cttagtattt ctgtttactt ttttatttgt agaatcctta
    10561 ttctggattt atcagttgaa caatgggata attggattat tgccaatttt tcagtatatg
    10621 gtaaattcca atccgtatgc attgatttat tggcttacat tactatctat cataattcca
    10681 ttgactgtat tttctgttca tagaaactgg aggagagtgt aaaagttgga aatgggaaag
    10741 ttaagtagtc acatgtggag gttgaatcag ataatctata ccaagtactt ttggggttat
    10801 gttctttttt ggatattgat ttgtttagga ttatggtatt ggttagaagg aaatgataga
    10861 cttgttatag aaattttaaa agggcctaat ctgagtcaaa actctttttt agtcttatct
    10921 atatggttgc ttcattggtt tattattcat acattttttc tagcagttgt atatcgtaga
    10981 agagcatccg atttctttat ggaagtgatt cgattttctt ctattaagct ctggattagg
    11041 tatcagattt ggacctgttt tctttatgga ctcattttaa tcatggtaaa agttctagtg
    11101 attcaattta tgttacagtt accaaactgg gatataggag ttttgtttat agttgattct
    11161 ttgaatgctt gtgtgttagt cttgttttgc tttatgttat acgcactagg agcgaatgta
    11221 caaatgaact ttgcttgcgt tagtttcttt ttactcatga ttgtgtttgg tggtctattt
    11281 gtaggaaatc gaactaatta tctattttat attttgaatc gagggaatgg agatatagga
    11341 cgagatttat ttcttcagtt gctattttta gtctttcttt ttaaaagtat tttctatttc
    11401 actcgtcaaa agaggaggtt tatagaatga atgaaataat catattaaaa aatattgagt
    11461 tgaaattaaa aaaaacatgt gtttttcaaa accttaattt tagttgtaaa cagggggaaa
    11521 ttataggaat tactggtgcg aatggctcag ggaaaagtgt attgtttaaa ttaatagctg
    11581 gtttatatag tccgtcttat ggagaagtgt taatcaatgg ggaaaatatt gttcctgaga
    11641 gaaaaattcc agctaatttg ggagctttga ttgaagaacc tggttttata aattattata
    11701 gtggctttaa gaatttacaa tatttggcaa gcatacgagg agtagttggt aatcaggaaa
    11761 tcaatgatac actgaaaata gttggtctat atgagcaaaa agaccagaaa gttaaaactt
    11821 attcgctagg tatgaggaaa aagctaggga ttgctcaagc aattatggag aatccctcta
    11881 ttcttttact agatgaacct atgaatgcct tggataaatc aagtgtagaa aatatgagaa
    11941 cattgtttag aaagctctct agtgaaaaag ggacaacaat tttgattgct agtcatagtg
    12001 aagaggatat tcgtatctta tgtgataaag tatatgcaat agaagataaa gtatgtacac
    12061 tgtgttcaga ttaaacatct tagatagact caggatgtta taggtaaaat cttaaaaagg
    12121 gattggagct taaaatccag tcccttttaa gagttaatgg ccaccttcaa caaccaggtc
    12181 atctgcttta gcagcgtcac cgtaggttgg gtgaagtgtt ttttcatagg ccttgtggaa
    12241 gaagttttcc ttgcctaatt tttcaatatc tttattgatg aagtctagca attcttggtt
    12301 gcctttttga actgctgccg caatggtatc gggatcaccg agggaagtaa ttcctacttc
    12361 aaatccttta ttttcaagtg cccaagctag aacttccgta ttgtcagttg aaaaggcatc
    12421 tccacgtccg tcaagaagag cttggtaaga gtcactgtat tggtcgtatt tttggagttt
    12481 gatttctgga tgattctttt caaaataagt ctcagcagtc gttccttttg tgacaattaa
    12541 ggttttacct tcaagttgtt tgacgtctgt aatgagacca gtcttaggtg atacgacacc
    12601 cagagaaact ttcatatatg gaagggcaaa atcaacttgt ttcttacgtt cgtcagttac
    12661 tgtaaagtta gcaagagtaa tatctacctt gtttgaaatc aagtattccg cacggttggc
    12721 agcatcgact gaaatgtatt taaccttgac accaaggtct tgagctagtt ggttccctag
    12781 ttcaatatcg tagcgtagcc ttggtaagaa ccatcattgt caacgtagcc aaacggtttt
    12841 ttatctccaa acacggcgat tcgcagttca ccgctttttt ttgatttcat cgatagtgcg
    12901 agctttggca gttgtttttc cagaggatga accagcgttt ccacctgagc tacaagcagt
    12961 gataaagata agggcaaagg caagtgctaa aacagttagg agtggtttga atagtttcat
    13021 aagaatctcc tttatagata tgagccaaat tggctaaagt caaagacgtt taaaaattcc
    13081 tgggctcgtt tggtttgcgg attggtaaag aaggcttgag ctgttccttc ttcagcgatt
    13141 ttcccttggt cgaggaagat aatccggtca gcaatggctt gggcaaactg catttcgtgg
    13201 gttactaaaa tcatggtacg gccttcttgg gccaaatcat tgataagttc cagcacctca
    13261 cgcaccattt ctggatccag cgaagcagtc acctcgtcaa aaaggatgat ttctggatgc
    13321 attaggaggg cacggacaat tgcaacacgt tgcttctgtc caccagataa ttgacgggca
    13381 aagctatgtt gtttatccag caaaccgaca cgatctagta attgcaaagc ttcttccgtt
    13441 acttctttct tgtcccatcc ttgagttttg atagggccta ggatgaggtt ttgtaagaca
    13501 tccagatggg gaaagagttc ataactttaa aagaccatgc caatcttttg gcgaactagg
    13561 tgaaaatctt ttttattttc aacgatagac tgaccatcca gaagaatgtc tccaccttga
    13621 atactttcta agccgttgag gcaacgaagg agggtacttt tcccacaacc agatggccct
    13681 aggatgacaa caacttcccc ttttttgatt tctagagaaa gtccttggag gatgggattg
    13741 tctccgaagg atttttttag ttccttgatt tctaagatag tttcagacat ttagtttctc
    13801 caatgttttt ctaagtgagt ggatagtttg gaaatagggt agcaaactgc gaaatataag
    13861 attagaatgg ttccataaat ccaaaatgaa gcagttggga tggtcaggcg attgctatcg
    13921 atgatttgtt gtccaacttt ggtcacttcc acaaccccaa tcaaaacaac taatgaagtg
    13981 gttttaatca tccgagtgac aagattgata gcctgcggta gcagtcttct taagacttgt
    14041 gggatgatga tgtggtagta aagttgaaca ttagtcaagt cgagtgccta tccactttca
    14101 aactgatgtt tagggagaga agtgatagct ccacgtacca agtctcccat ttcagctgtt
    14161 ccccagaggg taaaaatgat aatagctgaa gtctcacctg agatattgat attaaagttt
    14221 cgagccaagc caaagtaaac gatgaagagt agcaccagct ggggcatgat acggataaat
    14281 tccagataca atcgtgttaa aaatcgtatg attctagaat gggaggtcat gatgattccc
    14341 atgactgttc cgaacatcat ggataagagg acagacagga tggatatccc aatcgtaacg
    14401 cccaacccct gtaagattct caggagatta tttccttgaa agagtacttg gattcccgaa
    14461 tcctgcatgg cgggtcctcc tttctatcca gctaaagacc agtgagatgg gtagcagcat
    14521 gatcagataa gcaactacca acatagctag cgcaatgtct gtctcatagt agagaccaat
    14581 caaatccttg gcgacgtaca tgaggtcggc caaagccact gctgagaaaa cagaggtttc
    14641 cttgataagg aaaatgacat tggcactaaa ggaggggagt gccaccgctg ttgcttgcgg
    14701 aagaaccaca tagcgaaaga cctgtagagg tgtcagacca atagcgaggc caatctcctg
    14761 ctgggtttga ctgatggctt ccagcccact tcggaaagat tctgccatat aggatcctcc
    14821 taaaaagaca agcccaagcg ttgcacagac ttctgaagat aggacaatcc cgattcgggg
    14881 aagaccgaag tagagaaaga agagttgaat caaaaggggc gtattacgtg acaattcaat
    14941 gtaggctgtc gctacttgcg ccaaaacagg gattcgataa tgtcggatga tactaacgat
    15001 taaaccgagc agaaaggatc ccaaaattcc ccaaactgca atatgcaagg tcagaaagaa
    15061 tgccttttga tatagtggta gatattgttc aacaatggac caatccaaaa atagaacctc
    15121 ccatctagaa ataatacagt tattgtagca cttaaaatct tctttggata atatctattt
    15181 tttattgccg ttataaggat tttttaccat agacataaaa tttctgaaat ttccaaacaa
    15241 aatatttgaa aagttttgaa aaaaagttaa gatatttttg taatatacaa agtaaacgct
    15301 tacttattaa agaggacagt ttatgtcata caaaacaagc aatgcagaag gtcatgtaga
    15361 tttcatcaat acctatgatt tggagacaat ggcgcaacaa gttattccta aagcagcatt
    15421 tggctatatc gctagtgggg cggaagatac tttcgcttct ttccagtgat tttagcgtca
    15481 ggttcttttt agtttttaaa gattatccgt gaatttctta cttatttatg ataaaatggg
    15541 agtatcgcaa aaaatgactc atcgtattca attttgagta aaactaggag gatcccatgt
    15601 ctacagaaca tatggaagaa ctaaatgacc agcagatcgt tcgccgtgaa aaaatggctg
    15661 cgctccgcga acaaggaatc gatcctttcg gaaaacgttt tgaacgtact gcaaattcac
    15721 aagaattaaa agataaatat gccaacctcg ataaagaaca attacacgat aaaaacgaaa
    15781 cagctactat cgcaggacgc ttggtaacca aacgtggtaa aggtaaagtg gggtttgccc
    15841 accttcaaga ccgcgaaggc cagattcaga tctatgttcg taaggatgct gtcggtgaag
    15901 aaaactacga aatcttcaaa aaagcagacc ttggtgactt ccttggtgtc gaaggtgaag
    15961 tgatgcgtac ggatatggga gaactctcta tcaaggcaac ccacatcaca cacttgtcta
    16021 aggctcttcg tcctcttcct gagaaattcc atggtttgac agacgttgaa acaatttacc
    16081 gtaaacgtta ccttgacttg atttctaacc gtgaaagctt tgaacgtttt gtcactcgtt
    16141 caaaaatcat ctctgaaatc cgtcgttacc ttgaccaaaa aggattcctt gaagtggaaa
    16201 cacctgttct tcacaacgaa gccggtggtg ctgctgcccg tccatttatc acccaccaca
    16261 atgcccaaaa cattgacatg gtgcttcgta tcgcgactga gcttcactta aaacgcctta
    16321 tcgtgggtgg tatggaacgt gtctatgaaa ttgggcgtat cttccgtaac gaaggaatgg
    16381 atgctactca taaccctgag ttcacttcta tcgaagttta ccaagcttat gcagacttcc
    16441 aagatatcat ggacttgacg gaaggcatta tccaacatgc tgctaaatca gttaagggcg
    16501 atggcccagt caactatcaa ggtactgaaa tcaagattaa cgaaccattt aagcgtgttc
    16561 atatggtgga tgctatcaga gaaattactg gtgtcgattt ctggcaagac atgactttgg
    16621 aagaagctaa agctatcgct gctgagaaga aagttccagt tgagaaacac tacactgagg
    16681 ttggtcacat catcaatgcc ttctttgaag agtttgttga agaaacttta atccaaccaa
    16741 cctttgtcta tggacatcca gtagctgtat ctccactcgc taagaaaaat cctgaagacc
    16801 aacgctttac tgaccgtttc gagctcttta tcatgactaa ggagtacggt aatgccttta
    16861 ctgagctcaa cgacccaatc gaccaactta gccgttttga agcccaagct aaagccaaag
    16921 aacttggtga tgatgaagcg acaggaatcg actatgacta cattgaagct cttgaatacg
    16981 gtatgccacc aacaggtggt ttgggaatcg gtatcgaccg tctctgcatg ctcctcactg
    17041 atacaacaac tatccgtgat gtattgctct tcccaacaat gaaataaatt cttatcctct
    17101 ggctcttacc agaggatttt tttattcaaa aagagactga atttaaggag aaaatgaagt
    17161 gtagtgtatt gaaattgaaa tagtacactt tgatttctaa gacattgtta gaaattggtt
    17221 taaattccct aatcaatttg tgcatgtttt atttcatttt acgatagtac gctgaaactt
    17281 ttcaaaaagt actagaaatt gacttggatt ccccaattga tttgttcaga ttcactataa
    17341 ataaaaaatt aataagtggg ataggaagtt agcgtcaact aggatagtat cttgcttaaa
    17401 cagtatatat gggactgata taagtccata ggtcctatta gaggatgttc tggtgtctta
    17461 ttcacttgtt ttttatagta ttagtagata gaatcagcaa ataaaaaagt agttcaaatg
    17521 cctggcagca aaaaatctca acgattcact taatatagac taaggtttag agtttaagga
    17581 aagtagggct agtatattgt atgaaaaaat aagatggttt ttaagcaaaa ctcttgaaaa
    17641 tgattgaaaa aagagttatt atagttgtat agtaaaaagc aaacgcttac ttaatcgagg
    17701 aggacagttt atgtcataca aaacaagcaa tgcagaaggt catgtagact ttatcaatac
    17761 ctatgatttg gagccaatgg cgcaacaagt tattcctaaa gcagcatttg gctatatcgc
    17821 tagtggggcg gaagatactt tcactttaag agagaatatc cgcgccttta accacaaact
    17881 catcgttcct catacacttt gcgatgttga aaatccaagt acagagattg aatttgcagg
    17941 tgaaaaacta tcttcgccaa tcattatggc acctgttgcg gctcataaat tggcaaatga
    18001 acagggggaa gtggcgactg cgcgtggtgt gcatgagttt ggttctcttt atacaaccag
    18061 ttcttactct actgttgacc ttccagagat ttctgaagcc cttcaaggga cacctcattg
    18121 gtttcaattt tactttagta aggatgacgg tatcaaccgc cacatcatgg accgtgtgaa
    18181 ggatgaaggt tataaagcga ttgtcttgac ggcagatgct actgtagggg ggaatcgtga
    18241 agtggataag cgtaatggtt ttgtcttccc agttggcatg ccgattgttg aagaatacct
    18301 gccagaaggt gctggtaaat caatggactt tgtttacaaa tcagctaaac aacgcttgtc
    18361 tccacgcgat gtagaattta tcgctgaata ctctggtctt cctgtgtatg tcaagggacc
    18421 acaatgccgt gaggacgttg aacgttcgct tgctgcagga gcttctggta tctgggtaac
    18481 caaccacggt ggtcgtcaaa tcgacggtgg accagctgcc tttgactcgc ttcaagaagt
    18541 ggcagaagca gttgatagac gtgtgccgat tgtctttgac tctggtgttc gtcgtggtca
    18601 acacgtcttt aaagccttgg cttcaggagc agacttggta gctattggtc gcccggttat
    18661 ttatggcttg gctctcggtg gtagtgtcgg tgtgcgtcaa gtctttgaac acttgaatgc
    18721 ggaattgaag acagttatgc aattgtctgg agctcagacc attgaagatg tcaaacactt
    18781 taagctccgt cacaatccat acaacccaac cttcccagtt gaccctcgtg acttaaaatt
    18841 gtattgataa aacagattgc ctccactgaa tgtggaggtt tttcttcgtg tattggaaga
    18901 ataaatcgct atcgaaaacc atagtaaaat ggaataaaaa caggacaaat caatcaggat
    18961 atttaaatcg atttctaaca atgttttaga agtagagggg tactattcta gtttcaatct
    19021 actatatata aagatatttt tcggttaaat atcttgctaa ggctttcatt ttttgctata
    19081 ctggttcagt aattaaataa tagagacatt tggggtgctt taggctgaga taatacccat
    19141 tgaacctgat acagttaaga ctggcgaagg gaaatgtgaa cagttttttc gtatggtgaa
    19201 atgaacggtc taatcttgta agtcaactct aattcttgat tgtaattgga gttttttgtt
    19261 tatataaact ggataggctt gtttagttag ctctctgcaa atactctcga tgtgttttaa
    19321 aaggatcaag tggaattacg attttgcttg atatttcttg gtgttttact aattactaac
    19381 aagtaaagag aggagaagta aatatggaaa cacaagacta tgcatttcag ccaggtttga
    19441 ctgttggaga attattaaaa agcagtcaga aggattggca ggctgcaatc aatcatcgtt
    19501 ttgttaagga actttttgcg gggacaattg agaataaggt cttaaaggac tatctgattc
    19561 aagattatca cttctttgat gccttcttat ccatgctggg tgcctgcgta gctcacgcag
    19621 ataaacttga atccaagctt cgttttgcca agcaactagg ctttcttgaa gcagatgaag
    19681 atggttattt ccaaaaggct tttaaagagt taaaagtagc agagaatgac tatctagaag
    19741 tgactttgca ccctgtaaca aaagcctttc aggatctaat gtattcggca gtggcttcat
    19801 cagactatgc ccatcttttg gttatgctgg tcattgcaga aggtctctat ttagactggg
    19861 gttctaaaga tttggctcta cctgaagtct atattcattc ggaatggatc aatctccaca
    19921 gaggtccttt ctttgcagag tgggttcaat ttctggttga cgaactcaat cgtgtcggta
    19981 aaaatcgaga agatttgaca gaacttcagc aacgctggaa tcaagcagtc gctttagaat
    20041 tagctttttt tgatattggt tacgatgtct agagaaggct catgatattt acaaatttga
    20101 tgaggtaata ctcttcgaaa atctcttcaa accacgtcag tttcaccttg ccgtaggtat
    20161 ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
    20221 cagtcttatc tacaggttca aagcagtgtt ttgagcaatc tgcggttagc ttcctagttt
    20281 gctctttgat tttcattgag tataaatcat tcaacgtaaa tgaaaaaaat ttcttaacga
    20341 aagatagaga taaatcgaaa tcagtagatc gtataaagtg aaaaggaagg atttcaatat
    20401 gacaagttta aaattattaa aaaaaaaagc accattggtc atttgtataa ccaatgatgt
    20461 agtaaaaaat ttcacagcaa atggattagt agcactgggc gcctcaccag ccatgagtga
    20521 gtttccagca gatttagagg atttgttaaa gtatgctggt ggtttattaa taaacatagg
    20581 aacattgaca gatgaaaatt ggaaattata ccaagctgct ctgaaaattg cggagaaata
    20641 taatgtccca gcagttttag atcctgtagc ctgtggagca ggagaatata gaaaaaaagt
    20701 agcagatgat ctaatcaaca attataaact agcagcgatt agaggaaatg ctggcgagat
    20761 tgcctcttta gtaggaatag atgtggcatc taaaggagta gatagtgcgg gcgtagataa
    20821 tattgacgaa attgctctag cagcaaatga gaagttcaat attccaatag tagtaacagg
    20881 tgaagtggat gccattgctg ttaatggaga agtggtaacg attcataatg gtagtgctat
    20941 gatgccgaaa gtcattggga caggatgctt attaggagct gtagtagcaa gttttatcgg
    21001 actggaaaaa ggtcaagaat taaaatcatt agaaacagca atgttagttt acaatatcgc
    21061 tggagaaatg gcagaaaaac gtccaaatgg acatcttcct gggacattta aagttgaatt
    21121 tataaatgcc ttatacgaga ttacagatga agatgtaaag gaattcaaaa gagtgaagta
    21181 agatgtttca taaagaatta ctaaaactat attttatttg tggaacgact acttgccaag
    21241 gaaaaaatct atatacagtc gttgaggaag ccttaaaagg tggtataacc ttatttcaat
    21301 ttcgagaaaa aggtgagggt gctttagaag gcttagaaaa acttgagcta gctatccaaa
    21361 ttaaagaact ttgtaaaaaa tacaatgttc catttatcgt taatgacgat atagatttag
    21421 caatggaaat tgacgccgat ggcgtacatg taggtcaaga tgatattggt gttgatgaaa
    21481 ttagaaaatt gatgccagat aaaataattg gtctttctat aagaaacgaa gaagaatttc
    21541 aacaatcaaa agttgaatat gtagattatg taggtgttgg gcctgtattt gatacccagt
    21601 caaaagatga tgctggtggt gctataggtt atgaaggtct tgaattgatg agaaaactat
    21661 tgccacaaat gcctttagtt gcaattggtg ggatacagac gaaacatatt aaagacatta
    21721 taaagaccaa tatggatggc gtttcaatca tttcggcaat atcgtatgca aaaaatatag
    21781 aaaaaactgt tcgggaaatg agtgaacaat agatatctat ccttggattc taagtaagta
    21841 ggattttcct tttttaaaaa gctatgtcca gattcagctt gaatggctaa ttcatcaaac
    21901 aggtcatttg ttttttgcaa aagaaagatc tgctgtcttt gtgagtgcaa ggctgtattt
    21961 ccagttcaat ttcttgaact agtataaaat gtgtgttata ataatcacat aattaaataa
    22021 taaagacatt tggggtgctt taggctgaga tgatacccat tgaacctgat acagttaaga
    22081 ctggcgaagg gaaatgtgaa atgttttttt cgtatgtcga aaagaactgt ctaatcttgt
    22141 aagctgaact ctaattcgta tctgtaatta gagttttttc ttttacgaaa ctggatgaag
    22201 atgatacagt agattgaaat aagatgcgaa caaatcaatt agaaaattca aattaatttc
    22261 tagaaatatt ttagtgttct tgtgtactgt tctaaattca gtctgctata caaagtattt
    22321 aggtcggtct tcttgagtgc cccacttttc tttaaaaaaa ggagtttttt atgttgaaaa
    22381 aatggcagtt aaaagatgtt atcttgcttg ctttcttgtc tatctttttt ggtggggttt
    22441 tcgttggttc aggatatgtg tataatattc tcagtctact cttaacacct cttggtttgc
    22501 aggcctttgc caatgaaatc ctcttcggtc tctggtgtat ggctgcgccc attgctgcca
    22561 tctttgttcc gagagtcgga agtgcaacga ttggagaagt gctagctgcg cttgctgaag
    22621 tcctttatgg tagccaattt ggtctaggag ctcttttgtc tggcttggtt caaggtttgg
    22681 gaagtgaatt tggttttatc gtaactaaga atcgctatga aagttggctc tctctaactg
    22741 ctaatagtat tgggattacg cttgttagct ttgtctatga atacattaag ttaggttact
    22801 acgccttttc ccttccgttt gtcctttcct tgcttgtggt acgttttatt tctgtttatt
    22861 tcttctgtac catcttggtt cgtgccattg tcaaactcta tcatcagttt gcaactggag
    22921 gaaaagcata gatgggtctg gaactacgag cgattcagtc cccaattttc tctgagccgt
    22981 ttgattttac ttttcatgcg caagccttta ccttgttagt tgggagcagt ggttcaggaa
    23041 aatccagcct ctttcaaatg attgcccaag tcagttctct tccctatagt ggtcaagtcc
    23101 tgatagatgg gagcgaggtc agtcagcttt ctatcatcgc acgtgtccag aaggttggca
    23161 ttctcttgca aaatctcaat catcaattta ccatggagaa cttgtttgag gagttgattt
    23221 tcaccttgga gaatatcggc tatcaccttc aggaaattga ttctaaaata gcagaggttg
    23281 tccagcaatg tcgttgcgag gcaatcttac accgtcccat tcatcaccta tcaggtgggg
    23341 aaaagcaaaa agcagcgctg gctgtcctct ttgccatgaa tcctagggtc tatctcttgg
    23401 atgagccctt cgcttccatt gaccgcaaga gtagaatcga gatattggag attctaaaag
    23461 agttggctct tgatgggaag acagtcattt tgtgcgacca tgatttatct gactataaag
    23521 cctatatcga ctatatggtt gagctaagag acggaaaact aagggaagtg tttcaaatcc
    23581 cttcctatga gatgacacag gttgcttcaa aggaagttgc ttctagcccg gaactattcc
    23641 atatggatag aacgacttgt gagtttggaa atcgctccct cttttcaatt gctgatttca
    23701 cattctatca agggatttcc tgtatcctgg gtgacaatgg tgtcgggaaa tcaaccctct
    23761 ttcgctctat tcttcaattt caaaagtata aggggcgcat tgcatggaag gggacagtcc
    23821 tgaaaaagaa aaagagtttg taccgtgatc tgacgggtgt tgttcaggaa gctgagaagc
    23881 agtttatccg agtcagtctg cgagaggagc ttcaattaga tggacctgat tctgaaagaa
    23941 atcagcggat ttttcaagct ttacgatatt ttgatttgga gcaggcagtc gataagagtc
    24001 cctatcaatt aagtggtggt cagcaaaaaa ttcttcagct cttgaccatc ttgaccagta
    24061 aggcttccgt gatcttgcta gatgaacctt ttgcaggttt ggatgataga gcctgccatt
    24121 atttttgcaa gtggattgtg gaggagagga atcaaggaag aagttttctg ctcattagtc
    24181 atcgtttaga ccctttgatt tctgtggttg attattggat tgagatgact agtcagggtc
    24241 ttcggcatgt gaaagaagtg accattacca aaccacttac atctcagagt agcaataccc
    24301 aaggggaggt gagatagtat ggtcaaagta gcaacccaga caccgattat cagtctcttc
    24361 ttgctgattt tatccttgga aacatctttc attccttcga ttgctctgaa tctttcggta
    24421 gtcgcatttt gtattctctt tatgctctat taccgtcgat ttaaaatgtt agcttggatg
    24481 atcatacttg ccattttacc atcttttgcc aactactggg cagttcagtt acacggagat
    24541 gcttcacagg cagtcatgct tggaacgagg gcctttgtga cagtttgtat cggccttgtc
    24601 tttgtttcct ctatttcact aaaagagctt ctcttgtact tggctcaaaa ggggctatca
    24661 cgctcttggt cctatgcctt gattgtggta ttcaattctt ttcctctcat tcagcaagaa
    24721 atcaagtccc tcaaagaagc ttgcctatta cgtggtcaag aactacattt ttggtcgccc
    24781 ttgatttaca gtaaggttct gatgacagtc tttaggtggc gccatcttta cctgagagct
    24841 ctatctgctc acggatatga cgaacatgca cagttgaaga atagctatcg gactttttat
    24901 attcctaaaa aaacaaaatt aatctacctg cttttctttt tattgcttca aaccagtcta
    24961 tttttataaa ggagttatta tggaatttac agatattgcg atggaattat ccaagaaagc
    25021 ttggcaggct tcctttcatc atccctttat tttacaatta caagagggga atttagaacc
    25081 tgccattttc cgctattacc tgattcagga tgcctactat ctgaaggcct tctcagaaat
    25141 ctatcatctt ttggctgata agacttcaaa ccaagagatg aaaagactct tgaaacaaaa
    25201 tgctcagggt ttagtggagg gtgagttatt tattcgccaa caatttttca aggaattgga
    25261 aatcagcgac caggaaatgg agcaacatcc aatcgctcca acctgttatc attacatttc
    25321 tcatatttat aggcaatttg cagagccgaa cttagccatc gcttttgcaa gcttgcttcc
    25381 ttgtccttgg ttataccatg atataggcaa atcacttaat cttaaaccat caccaaatcc
    25441 tctctatcaa caatggattg aaacttatat tacggatgag ttagagcaac agatcagaga
    25501 ggagggagca ctggtcaatc agctctatcg agaaagtgat gagacagata agcaaaaaat
    25561 gctagatgcc ttccacatca gtgttcatat ggaagccaag ttttgggaga tggcttacca
    25621 acaccagaca tggaagagcg atttacagtc tttagaaaaa ggagaagaat agaaacatga
    25681 gaaagcacca actacaagtt cacaaattga ccattttatc tatgatgact gcccttgatg
    25741 tagtccttac acctatcttt cggattgagg gaatggcacc gatgtccagt gtagtcaata
    25801 ttctagcggg aatcatgatg ggacctgttt atgccttggc catggctaca gtgacagcct
    25861 ttatccgtat gacgactcaa gggattccgc ctttagctct cacaggagcg acttttggag
    25921 cccttctagc aggtctcttt tataagtacg gtcgaaaatt ttacttttct gccttgggag
    25981 aaattgtggg aacaggtatt attggttcta ttgtttccta tcctgttatg gtactcttta
    26041 caggatcagc tgctaagctt agctggttta tctacacgcc tcgatttttc ggagcaacct
    26101 tgattggtac agcgatttcc tttattgcct ttcgattttt aatcaagcag gaattcttta
    26161 aaaaagtgca gggatatttc tttagtgaaa ggatagactg atgcaggaat ttacaaatcc
    26221 ctttcctata ggctctagtt ccctcattca ctgtatgacc aatgagattt cttgtgagat
    26281 gctggcaaat gggattttgg ctctgggatg caaacctgtc atggcagatg atccccgtga
    26341 ggttcttgat tttactaagc aaagtcaggc tctcttcatc aatttggggc atttgtcagc
    26401 tgagaaggag aaagcaatcc gtatagcagc ttcttatgca gctcaagttt gtctcccaat
    26461 ggtagtagat gcggttggcg taacagcttc atccattcgt aagagcttag tcagagacct
    26521 tttagactat agacctacgg tccttaaagg aaacatgtca gaaattcgaa gtcttgttgg
    26581 attaaagcac cacggcgttg gggtcgatgc gagtgctaaa gatcaagaaa cggaggattt
    26641 gcttcaagtc ttgaaagact ggtgtcagac ctatcatggt atgtctttct tagtcacagg
    26701 tcccaaggac ctcgtcgttt cgaaaaatca gatcgctgta ctgggaaatg gctgtgcaga
    26761 attagactgg ataacaggga caggagattt ggttggagcc ttaacagccg tttttctcag
    26821 ccaaggaaag actggttttg aagcttcttg cttagcagtc tcttatctca atatcgctgc
    26881 tgagaaaata gttgttcaag gaatgggatt ggaagaattt cgttaccaag tactcaatca
    26941 gctttcgctc ctaagaagag atgaaaattg gctagatacc atcaaaggag aggtttatga
    27001 atagagaagc acttagacta tatctggtaa ccaatcgcta tcaagattcc gtggaaagct
    27061 ttcttgcaaa agttgagacg gcctgccgtt caggggttac catagtccaa ttgcgagaaa
    27121 aaaatctcac aaccaatcaa tattatcaac tggcaaaaca agtcaaggaa ataacagatg
    27181 cttatcaggt acccttgata atcgatgatc ggttggatgt ttgtcttgcg gttgatgcag
    27241 caggtctgca tatcggagat gatgaattac cagtttcggt agctcgcaaa gtcttgggtc
    27301 ctgaaaaaat cctcggtgtc accgctaaaa cggttaaaag agctctggaa gcggagaaat
    27361 caggtgcaga ttacttgggt acaggagcca ttttcccaac caccaccaag gaaaatgcgc
    27421 ccattactct gatatcgacc ttgaaaacaa tttgccaaac ggttgccatt ccagtagttg
    27481 ctattggagg cttgacttcg gagaatattg atcagcttat gggcactggc atagctggtg
    27541 tagctgttgt acgtgatttg atgcaagcag aagatattga ggcaaaaacg caagcctttt
    27601 taacaaagtt gcatgacatt ctttcctaat taatactcaa tgaaaatcaa agagtaaact
    27661 agaaagctag tcgcaggttg ctcaaagtac tgccttgagg ttttagataa gactgacgaa
    27721 gtcagctcaa aacagtgttt tgaggttgca gatggaagct gacgtagttt gaagagattt
    27781 tcgaagagta taaaaactct ctaattccct taaagtggga actagagagt ttttttctta
    27841 atctttaaaa gttgtatggt tgactggacc agaaccatga ccgagttgag gggcatcttg
    27901 aatagctttt gtgataaagg ccttggcctt atcaactgcc tggtaaagac tcttgccctt
    27961 ggctagttca gcagtaatca ctgcagcaaa ggtacatcca gtaccatggg tgtgacaggt
    28021 ttgaattcgt gggctttccc agacaaattg ttcattcttg gtaaagagga aatctttagc
    28081 accgcctttg agatgtccgc ctttgataac cacagactga ggaccaaatt cttttaaaat
    28141 caggcgacca gcacgctgca tgtcttcggg gtcatggatt gaaaaaccaa caatctcttc
    28201 tgcttcagga agatttggcg taataatagt tgctagaggt agtaagtttg ttttgagata
    28261 gtctctagca tttgagccaa tcaaggcatc tccacttgta gcaaccataa caggatcaag
    28321 gacataggga caatccagtt ttttaagata gggttggatg atttccatga tttcagtagt
    28381 agccaacatt ccagttttta cagcctgagg tggaatatca gaaaagacac tctccaattg
    28441 ggctttcaac atttgaggag aaacgtgctc gattagctga acacctctgg tattttgagc
    28501 gacaagactg gttacaacag ccattccata gacatctctc gcttggaatg actttaaatc
    28561 tgccataatg ccagcaccac cactagggtc agtccctgca atggtcaaag caacgggtaa
    28621 ataagtcatc taaaacctcc caaccaactg aagtttgtat tcttaagaac aagctagtct
    28681 gttttagaaa ggcaatagaa gactgctagt ccccattatc atttccactt ccatcagcta
    28741 gcattaccta gattgagtca gagggatcta acagtcgtta atctcagctt tacgcacccc
    28801 tagtggttgt tttctttttt ctttagtata gcatattttt attgtttata tagtaatatt
    28861 tgactgcaaa tcgtttttca aatgattaaa attctatttt tcgaagaaaa tattatttct
    28921 ataattgaca aatgtagatt ttaagagtat actgatgagt gtaattgaca aatgtagatt
    28981 ttggaggtat ggtaatgcag atttcagatg cagaatggca ggtcatgaag attatttgga
    29041 tgcaggggga gcagaccagt acagatttga ttagggtttt ggcagagcgg tttgactggt
    29101 ccaagtccac ggttcagaca cttttggctc gtctggttga gaaagagtgt ttgactcgga
    29161 aaaaagaagg caagtccttt gtttattcag cccttttaac tctagaccaa agtcgggatt
    29221 tacttgtcca agatatcaaa gacaaggttt gttcccgtag gattaggaat ttgttggctg
    29281 acttgattgt agaatgtgaa tttactcaga ctgacttgga agacttggaa gctgtgattt
    29341 cagagaagaa atcaagtgct gtaacagaag taagatgtaa ttgtatgtaa aggagacgtc
    29401 atgttaaata gtattgtaac cattatttgt attgccctta tcgcgtttat cttgttttgg
    29461 tttttcaaaa agcctgaaaa atctggacaa aaagcccagc aaaaaaacgg ataccaagag
    29521 attcgagtgg aagtcatggg aggctatact cctgagttga ttgtcctcaa gaaatcagtg
    29581 ccagcacgca ttgtctttga ccgcaaggat ccttcaccat gtctggatca aattgttttt
    29641 ccagattttg gtgtacatgc gaacctgcca atgggggaag agtatgtagt ggaaatcacg
    29701 cctgaacagg ctggagagtt tggctttgct tgtggtatga acatgatgca cggcaagatg
    29761 attgtagagt aggtggagac tatgacagaa attgtgaaag caagcttaga aaatggcatt
    29821 caaaaaatcc gtatccgagc tgaaaaaggc tatcatccag cccatatcca gcttcaaaag
    29881 ggaattccag ctgagattac ctttcatcgt gctactcctt caaactgtta taaggaaatt
    29941 ctgtttgaag aagaaggtat cttggaacca atcggcgtag atgaggagaa agtcattcgt
    30001 tttacgcctc aagaattagg gcgacatgaa ttttcttgtg gcatgaagat gcaaaaggga
    30061 agctatacag tcgttgagaa gactcgaaaa tctctatctc tcctgcaacg tttttggatt
    30121 actagtatct ttactgtgcc tcttgtgatt ctcatgattg ggatgttgac aggtagcatt
    30181 agtcatcaag tcatgcattg gggaaccttt ctagcaacaa cgcctattat gttagttgca
    30241 ggtaagccat atatccagag tgcttgggcc agttttaaaa agcacaatgc caacatggat
    30301 accttggttg cgctgggaac tctagtggct tatttctata gcctagttgc tctctttgct
    30361 ggtctccctg tttacttcga aagtgctgga tttatcctct ttttcgttct tttgggagca
    30421 gtttttgagg aaaaaatgag aaaaaatacg tcccaagctg tggagaaatt actggacttg
    30481 caagctaaaa ccgcagaagt cttgagtgat gatagttatg tccaagttcc tttggaacaa
    30541 gtcaaggtag gcgacctgat tcgagtgcgt cccggtgaaa agattgctgt tgatggtgtc
    30601 gtagtagaag gtgtctctag tattgacgaa tccatggtga caggtgagag tctgcctgtg
    30661 gacaagacag ttggagatac tgtcattggc tcaaccatca atcatagtgg aacgcttgtc
    30721 tttagagcag aaaaagttgg ctcagagact gttttggctc agattgtgga ttttgtgaag
    30781 aaagctcaga caagtcgtgc gccgattcaa gacttgacgg ataagatttc agggattttt
    30841 gtcccagtag ttgtcatttt aggaatcatg accttttggg tttggttcgt cttgctcagg
    30901 gatagtgtgg ttgtgcttgg agctagcttt gtgtcctctc ttctctacgg agtggcggtt
    30961 ttgattatcg cctgtccttg tgccttggga cttgcaacac cgacagccct tatggtgggg
    31021 acaggacgta gtgccaagat gggggttctc ctcaaaaatg gaactgtctt acaggaaatc
    31081 cagaaagttc aaacccttgt ctttgataag accgggactt tgacggaagg gaaacctgtg
    31141 gtaacagatg tcatcggcga cgaagtagaa gtgtttaggt tggcagcctc cttggaagat
    31201 gcttctcaac acccactggc tgaggctatc gttaagcgag cgagtgaagc tggacttgag
    31261 tttcaaactg ttgaaaattt tcagaccttg cacgggaaag gtgtttcagg gcgaatcaat
    31321 ggaaaacaag ttttacttgg aaatgctaaa atgctggatg gtatggatat ttctaatact
    31381 tatcaagata aactagaaga actagaaaaa gaagctaaga cagttgtgtt cttagctgtt
    31441 gacaatgaaa tcaaaggctt gcttgctttg caagatattc ctaaggaaaa tgctaagcta
    31501 gccatcagtc agctaaaaaa acgtggtctc cgaacagtca tgctgacagg agacaatgct
    31561 ggtgtggcgc gtgctattgc agatcaaatc ggaattgaag aggtcattgc aggcgtcttg
    31621 ccagaagaaa aagcccatga aatccataaa ctgcaacagt cagggaaagt agcctttgtt
    31681 ggggacggta tcaatgacgc tcctgccctt agtgtagcag atgtggggat tgctatggga
    31741 gctggaacag atatcgccat cgagtcagca gatttggtgt tgacaaccaa taatctttta
    31801 ggagtggttc gtgcctttga tatgagtaag aaaacctttc atcgaattct actcaatctt
    31861 ttctgggctt ttatctacaa tgttgtcgga attccgattg cagcaggagt cttttcaggt
    31921 gttggactgg ctctcaaccc agaattggca ggtctagcca tggcctttag ttctgtatct
    31981 gttctgacca gttcccttct gttaaatttt agtaaaatag actaaaagta atgactagat
    32041 ttctttgtta taaaacagaa atgacagaac tttcaaaact gtattagaat agagaataga
    32101 gagttttgag cagattttta gaaaagtcag cataaatatg atacagtgga atagtaaaaa
    32161 tttggagaac gtttccaatt ctatgtaatc gtattctcca aatctaaaaa aattgaagga
    32221 gagttatcat tatgactcaa gggaaaatta ctgcatctgc agcaatgctt aacgtattga
    32281 aaacatgggg cgtagataca atctacggta tcccatcagg aacactcagc tcattgatgg
    32341 acgctttggc tgaagacaaa gatatccgct tcttacaagt tcgccacgaa gagacaggtg
    32401 ctcttgcagc ggttatgcaa gctaaattcg gcggctcaat cggggttgca gttggttcag
    32461 gtggtccagg tgcgactcac ttgattaacg gtgtttacga tgcagctatg gataacactc
    32521 cattcctagc gatccttgga tcacgtccag ttaacgaatt gaacatggat gctttccaag
    32581 agcttaacca aaacccaatg tacaacggta tcgctgttta caacaaacgt gtagcttacg
    32641 ctgagcaatt gccaaaagta attgacgaag cctgccgtgc tgcaatttct aaaaaaggtc
    32701 cagctgttgt tgaaattcca gtaaacttcg gtttccaaga aatcgacgaa aactcatact
    32761 acggttcagg ttcatacgaa cgctcattca tcgctcctgc tttgaacgaa gttgaaatcg
    32821 acaaagctgt tgaaatcttg aacaatgctg aacgcccagt tatctatgct ggatttggtg
    32881 gtgttaaagc tggtgaagtg attactgaat tgtcacgtaa aatcaaagca ccaatcatca
    32941 caactggtaa aaactttgaa gctttcgaat ggaactatga aggtttgaca ggttctgctt
    33001 accgtgttgg ttggaaacca gccaacgaag tggtctttga agcagacaca gttcttttcc
    33061 ttggttcaaa cttcccattt gctgaagttt acgaagcatt caagaacact gaaaaattca
    33121 tccaagtcga tatcgaccct tacaaacttg gtaaacgtca tgcccttgac gcttcaatcc
    33181 ttggtgatgc tggtcaagca gctaaagcta tccttgacaa agtaaaccca gttgaatcaa
    33241 ctccatggtg gcgtgcaaac gttaagaaca accaaaactg gcgtgattac atgaacaaac
    33301 tcgaaggtaa aactgagggt gaattgcaat tgtatcaagt ttacaatgca atcaacaaac
    33361 atgctgatca agacgctatc tactcaatcg acgtaggtaa cactactcaa acatctactc
    33421 gtcaccttca catgacacct aagaacatgt ggcgtacatc tccactcttt gcgacaatgg
    33481 gtattgccct tcctggtggt atcgctgcta agaaagacaa tccagatcgc caagtatgga
    33541 acatcatggg tgatggagca ttcaacatgt gctacccaga cgttatcaca aacgttcaat
    33601 acgaccttcc agttatcaac cttgtcttct caaatgctga gtacggcttc atcaagaaca
    33661 aatacgaaga tacaaacaaa cacttgtttg gtgtagactt cacaaacgct gactacgcta
    33721 aaattgcgga agctcaagga gctgttggat tcacagttga ccgtatcgaa gacatcgatg
    33781 cagttgttgc agaagctgtt aaattgaaca aagaaggtaa aactgttgtc atcgatgctc
    33841 gcatcactca acaccgtcca cttccagtag aagtacttga attggatcca aaacttcact
    33901 cagaagaagc tatcaaagcc ttcaaggaaa aatacgaagc agaagaactc gtaccattcc
    33961 gtctcttctt ggaagaagaa ggattgcaat cacgcgcaat taaataattc ctctcgccga
    34021 aaatcaaata tgaaacttgt agactttatt ctttgatttt catagagcag aactttaaaa
    34081 gatcggagca atccggtctt tttatggagg agagtaaatg aatttaaatc aattagatat
    34141 tatcgtttca aatgttcccc aagtctgtgc tgacttggag catattttgg ataaaaaggc
    34201 agattatgct gatgatggtt ttgctcagtt tacgattggt agtcactgcc ttatgttgtc
    34261 acaaaatcat ttggttcctt tggaaaactt tcagtcagga atcattattc atatcgaggt
    34321 tgaggatgta gaccagaact acaaacggtt gaacgagctt ggtataaagg ttttacacgg
    34381 accaactgta accgattggg gaacagagtc cttattagtt caaggacctg ctggtctagt
    34441 gcttgatttt tatcgtatga aataggtcgc gtagtcattt gacgttaacc ttagctattt
    34501 ttaaaataga aattgagaat atgaactatt gaaagatcgg agcaatccgg tctttttata
    34561 taaagaaatt gcatat
//
LOCUS       DAVKWS010000020        31887 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKWS010000020 DAVKWS010000000
VERSION     DAVKWS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.020"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            136..1125
                     /gene="manX"
                     /locus_tag="VW852_000995"
     CDS             136..1125
                     /gene="manX"
                     /locus_tag="VW852_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV0272159.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1153..1956
                     /locus_tag="VW852_000996"
     CDS             1153..1956
                     /locus_tag="VW852_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV0272160.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1980..2891
                     /locus_tag="VW852_000997"
     CDS             1980..2891
                     /locus_tag="VW852_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0272161.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2947..4281)
                     /gene="pepC"
                     /locus_tag="VW852_000998"
     CDS             complement(2947..4281)
                     /gene="pepC"
                     /locus_tag="VW852_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV0272162.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4498..5220)
                     /locus_tag="VW852_000999"
     CDS             complement(4498..5220)
                     /locus_tag="VW852_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0272163.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(5533..5763)
                     /locus_tag="VW852_001000"
     CDS             complement(5533..5763)
                     /locus_tag="VW852_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0272164.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(5773..7014)
                     /locus_tag="VW852_001001"
     CDS             complement(5773..7014)
                     /locus_tag="VW852_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV0272165.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(7036..7188)
                     /locus_tag="VW852_001002"
     CDS             complement(7036..7188)
                     /locus_tag="VW852_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272166.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(7209..7487)
                     /locus_tag="VW852_001003"
     CDS             complement(7209..7487)
                     /locus_tag="VW852_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV0272167.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(7480..7743)
                     /locus_tag="VW852_001004"
     CDS             complement(7480..7743)
                     /locus_tag="VW852_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV0272168.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(7845..12236)
                     /locus_tag="VW852_001005"
     CDS             complement(7845..12236)
                     /locus_tag="VW852_001005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV0272169.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(12340..14421)
                     /gene="fusA"
                     /locus_tag="VW852_001006"
     CDS             complement(12340..14421)
                     /gene="fusA"
                     /locus_tag="VW852_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV0272170.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(14846..15316)
                     /gene="rpsG"
                     /locus_tag="VW852_001007"
     CDS             complement(14846..15316)
                     /gene="rpsG"
                     /locus_tag="VW852_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV0272171.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(15336..15749)
                     /gene="rpsL"
                     /locus_tag="VW852_001008"
     CDS             complement(15336..15749)
                     /gene="rpsL"
                     /locus_tag="VW852_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV0272172.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16166..20008
                     /gene="spuA"
                     /locus_tag="VW852_001009"
     CDS             16166..20008
                     /gene="spuA"
                     /locus_tag="VW852_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV0272173.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGTSLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDATSIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(20108..21157)
                     /locus_tag="VW852_001010"
     CDS             complement(20108..21157)
                     /locus_tag="VW852_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV0272174.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(21276..23084)
                     /gene="glmS"
                     /locus_tag="VW852_001011"
     CDS             complement(21276..23084)
                     /gene="glmS"
                     /locus_tag="VW852_001011"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV0272175.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERTSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(23277..24656)
                     /gene="bglA"
                     /locus_tag="VW852_001012"
     CDS             complement(23277..24656)
                     /gene="bglA"
                     /locus_tag="VW852_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV0272176.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(24755..26608)
                     /locus_tag="VW852_001013"
     CDS             complement(24755..26608)
                     /locus_tag="VW852_001013"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV0272177.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(26621..27880)
                     /gene="rseP"
                     /locus_tag="VW852_001014"
     CDS             complement(26621..27880)
                     /gene="rseP"
                     /locus_tag="VW852_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV0272178.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(27902..28705)
                     /locus_tag="VW852_001015"
     CDS             complement(27902..28705)
                     /locus_tag="VW852_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV0272179.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(28714..29472)
                     /locus_tag="VW852_001016"
     CDS             complement(28714..29472)
                     /locus_tag="VW852_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV0272180.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(29703..30275)
                     /locus_tag="VW852_001017"
     CDS             complement(29703..30275)
                     /locus_tag="VW852_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV0272181.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLCLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(30256..31227)
                     /gene="ruvB"
                     /locus_tag="VW852_001018"
     CDS             complement(30256..31227)
                     /gene="ruvB"
                     /locus_tag="VW852_001018"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV0272182.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            31388..31480
                     /locus_tag="VW852_001019"
     CDS             31388..31480
                     /locus_tag="VW852_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV0272183.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         9201 a         7464 c         6140 g         9082 t
ORIGIN      
        1 tatcaacata caaaaataaa aattttttaa ttttttatga taaatagttg ctttttttat
       61 gaaaacggtt tatacttaaa cagtcttaaa gttttcttaa atgaaaacta aaacaaaaaa
      121 ggaggaatga caataatgag tatcggaatc attattgcga gccacggcga atttgctgcg
      181 ggtattcatc agtcaggatc tatgatcttt ggtgaacaag aaaaggttca agttgtgacc
      241 tttatgccaa atgaaggtcc tgatgatcta tacgctaagt ttaataacgc tgttgctgca
      301 tttgacgcag aagatgaggt tctagttttg gctgaccttt ggagtggttc tccatttaac
      361 caagctagtc gcgtgatggg agaaaatcct gagcgtaagt ttgccatcat cacaggactt
      421 aacttaccga tgttgattca agcctacaca gagcgcctca tggacgctgc tgcaggtgta
      481 gaaaaagtcg ctgctaatat cattaaagaa gccaaagatg gcatcaaagc tcttccagaa
      541 gagctaaatc cagttgaaga agttgcaagc gctgcagctg ctccagttgc ccaaactgct
      601 atcccagaag gaactgttat cggagacggt aaattgaaaa tcaatcttgc ccgtcttgac
      661 acacgtctac ttcacggtca ggttgcaact gcttggactc cagattcaaa agcaaatcgt
      721 atcattgttg cttcagataa cgtggctaaa gacgaccttc gtaaagaatt gattaaacaa
      781 gcagctccag gtaatgtcaa ggctaacgtg gttccaattc aaaaactgat tgagatttca
      841 aaagacccac gttttggaga aacacatgcc cttatcttgt ttgaaacacc tcaagatgcc
      901 cttcgtgcca tcgaaggcgg cgtgccaatc aagactctta acgttggttc tatggctcac
      961 tcaacaggta aaacattggt caataccgtt ttgtctatgg acaaagaaga tgttgctaca
     1021 tttgaaaaaa tgcgtgactt gggtgttgaa tttgatgtcc gtaaagtacc aaatgattct
     1081 aaaaaagatt tgtttgactt gattaacaaa gccaatgtca aataagccat tatttatgaa
     1141 aggattttaa acatgtctat tatttctatg gttttagtag tcgttgtagc cttctttgca
     1201 ggtcttgaag gcatcctcga ccagttccaa tttcaccaac cacttgtagc ctgtaccctt
     1261 attgggcttg taacaggtca cttggaagca gggattatcc tcggtggatc gcttcaaatg
     1321 attgcccttg gttggtcaaa tatcggtgct gctatcgctc ctgatgctgc acttgcttct
     1381 gtcgctgctg ccattatcat ggttcttggt ggtgacttta ccaagactgg tatcggtgtt
     1441 gcccaagcgg ttgctatccc tcttgccgta gctggtctat tcttaactat gattgttcgt
     1501 acaatttcag ttggattggt tcacactgct gatgctgccg ctaaaaaagg tgacttcggc
     1561 gctgtggagc gtgctcattt cattgcgcta cttttccaag gacttcgtat cgcgcttcct
     1621 gcagctcttc tccttatggt accaactgaa actgtacaaa gtatccttag tgccatgcca
     1681 gactggctca aagatggtat ggctatcggt ggtggtatgg tcgttgccgt tggttacgcc
     1741 atggttatca acatgatggc aactcgtgaa gtatggccat tcttcgctct tggtttcgtt
     1801 ctcgctgctg tgtcagatat tactctaatc ggattcggtg ctatcggtgt tgctatcgct
     1861 cttatctacc ttcacctttc taaaactggt ggaaatggtg gcggaggagc cgcaacttct
     1921 aacgacccaa tcggcgatat cctagaagac tattaagata agaaaggact gaaaacatca
     1981 tgactgaaaa acttcaatta actaaatcag atcgtaaaaa agtttggtgg cgttcaacct
     2041 tcttacaagg gtcttggaac tttgaacgga tgcaaaactt gggctgggct tatacactca
     2101 ttccagctat caaaaaactc tatactaaaa aagaagatca aatcgctgct cttgagcgtc
     2161 accttgagtt cttcaacact catccatacg tagctgctcc agtcatgggg gttactcttg
     2221 cgcttgaaga agaacgtgct aacggtgtgg aaatcgatga cgctgctatc caaggggtta
     2281 aaatcggtat gatgggacct cttgctggta tcggtgaccc agtattctgg tttacagtac
     2341 gcccaatcct tggatctctc ggtgcttcac ttgcccttac tggcaatatc ttgggtccac
     2401 tcctcttctt tgttgcatgg aacttgattc gtatgtcatt cttgtggtat gttcaagaga
     2461 ttggatacaa ggctggatca gaaatcacta aagatatgtc tggtggtatc cttcaagata
     2521 tcactaaagg agcttctatc cttgggatgt tcattcttgc tgtccttgtt caacgctggg
     2581 taaatattaa atttgctttc gatgtttcta aagttcaact agatgaaaag gcttatatcc
     2641 attgggataa attgccagaa gggtctaaag gtatccaaga agcattcgca caagtaggac
     2701 aaggattgtc tcaaactcct gaaaaagtta ctactttcca acaaaacttg gatatgttga
     2761 ttcctggatt atcaggacta ctccttactt tactttgcat gtacttactt aagaaaaaag
     2821 tatctccaat cactattatc cttgccctct tcgcagtggg tattgtggca catgttcttc
     2881 acatcatgta atcaagcaac taaaaaggaa ccaggttcta aaatctgatt ccttttttct
     2941 atgcttttat tcagccaagg ctcccattgg atcccatggt gcaagtacga ttggttctgc
     3001 tccataggca gcttgttctt ctgctgtcag caattcctta cgaacaacga tttggtatgt
     3061 gtattcgtcc atccaagcgt ctgaggcaac aaagtaacca tctgtaccga ccttgtctcc
     3121 ccatgagttt tcaaccttcc acttggttga tttaccattt tcgtccaagt caacacctgt
     3181 caagaccatg gcgtgggtca tcaagctttc gctatagtcc aaacgtccag ccttgtcttg
     3241 agtaagttta atgtccatgc ttgattcaaa gtcataaaca tctgtcgcaa ggatgccagc
     3301 cttacggttg ctgagctggc cgacatcaga accaaaccaa acagtctcac ctgcttgcat
     3361 ttgggcaatc gccaattctt tcaagcgctc cattggaacg ttgatgtagc gaactgcacg
     3421 gctaccaacc acattcccca acatctcaac tgtgtaagat tttccgtaag gtttatcagc
     3481 agttggagca ttgataacag aaacgtagtc ttctaaagga agattgacat atttcttgta
     3541 aaactcttgt ggtgtgattc ctttttcact tttatagttg ttatctttat cgcgataagc
     3601 aaagtcaaac ttgcgtggtg gaagtcctaa tgacatagca agaaagttaa agatttcttg
     3661 caagaggtct tctttcttag cttgaacagt cgcttgatct gcaccagaaa caagcaaatc
     3721 acgcaagatt tgagcatctt gacgaagcaa tttattaagg atcgcattta gctcacgact
     3781 gctgctagat gaaacagact caggataaac tgacttaggc acgacaccgt atttttcaaa
     3841 gagggaaacg accatatccc attgaccgcc atcttgttga ggtgtttgga gtaagaagct
     3901 aaccttgcgg ctagtcaatt cttggtctga agtcgcaatg acttgctcca agaaccagtt
     3961 tgatttctca tacttatccc agaagaaagt gtgggcttgt gacaactcaa agttctccaa
     4021 tttgtattgc gagatgagtt tgtggcggaa ggtgttgaga gccgcaaaca tccagcaacg
     4081 accagacgct ttctggttag tgaccttgtc cttggttaaa tccaatgaga aaacaggtgt
     4141 gttgtctaca tggctttggc gacattccag agctgcaaaa attccgttgt ggctggcagc
     4201 attttcaatc gcttggtatt ttacatttgc ttcatagttg gcaaatagtt tatcagtaaa
     4261 tgattcttga atcgcgttca tagattcctc cttttattct acagtctatt ataactcttt
     4321 tcacaaagga agcaactaaa aaacataaaa ggcgaggaca ttgtccctca cctttttcga
     4381 atttatactc ttcgaaaatc tcttcaaacc acgtcaacgt cgccttgccg tactcaagta
     4441 cagcctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatta aactaaatta
     4501 aataatatta gcgcggagaa tttctaattc ttccttggtc aagcgacgcc attcccctcg
     4561 ttccaggttc tcatctaata ctaaagttcc catagtcaat cgttgcaagt ccaccacttc
     4621 cttgccacag tagcccacca tacgcttgat ctgatgaaac ttcccttctg caatggtcac
     4681 acggatttgg ctctgattct tttctgcatc tgtggaaaga atctccagtc tagcgggctg
     4741 acaggtaaag tctttgagcg gaatgccctt gataaatgtc tccacatctt cttgggtcat
     4801 aattcccttg acctgagcct gataagtctt gtccacatga cgcttgggcg aaagaagaac
     4861 atgagccagc tgaccattat tggtcaagag caaaagacca tgcgtgtcaa tatccaagcg
     4921 tcctactgga aaaacttcct tactccgcgc caagtcatcc aacaagtcca gaacggttct
     4981 gtgcttggga tcctcagtcg ctgagataac tcctttgggc ttgttcatca tgtagtagac
     5041 aaactcttca tactccaaca cttgcccatc aaagcgaatc tcatctattt tttcatcaat
     5101 ctgcaattta gctgattttt cttttttacc atttacagtc acgcgcccag ccttgagcaa
     5161 gtttttgacc tcagtccgac ttcccaccgc acaggcaact aaaaatttat ctaatctcat
     5221 agaactatta tatcatatca aaaggaggct agtacaatga ccaacctcct tttcgtttca
     5281 tactcttcaa aaatctcttc aaaccgcgtc aacgtcgcct tgccgtatat atgttactga
     5341 cttcgtcagt tctatctgca acctcaaaac agtgttttta gctgacttcg tcagttctat
     5401 ctgcaacctc aaaacagtgt ttttagctga cttcgtcagt tctatctgca acctcaaagc
     5461 agtgctttga gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatc
     5521 agatttagga aattaacttc ctcgtctcca aaaaatagct aaaacaatca tggctcctaa
     5581 aatcgctggg ataatagctg ttcctgataa aactggcccc caagttccaa agagcaagtg
     5641 acctagaaag gctccgatcc aaccgagaaa catttttcca aaacatccca ttcgctctcc
     5701 acgattggtc atagcacctg ctaaaaatcc cactaggaga ccaacgaaca tacttcctaa
     5761 catattatct ccttaatttg cccaattccc attacggaaa agaggtaccc gcgttccatc
     5821 ctcacgaata ccatcgatat ccatttggtt agaaccaatc ataaagtcta cgtgaacatc
     5881 tgaacggtta agccctgcag cttcaagctc ctcttcgctc atctccgctc catcaacaac
     5941 gctagtcgca taggctgcac cgatagccaa gtggtttgac gcattttcat cgaaaagggt
     6001 gttaaaaaag gtaatgcctg actgagaaat tggacttgga tctggtacca aggcacattc
     6061 acccaaggca cgcgcacccg cattttcaaa gacaaggtct ttcataacct gatcaccctt
     6121 ctcagcagtg atatctacga tttgtccatc cttaaaggtc accttaatgc cttcaatgat
     6181 atttccgttg tagctaagcg gttttgtaga agtgacataa ccatctgcac gacggaagtc
     6241 aggcgctgtg aagacctctt ctgtcggcat atttggcaag aattcttcgc cctgtgcatt
     6301 gacagcacca gctgattccc aaacgtggtt ctttggcaaa ccaagtgtta aatctgttcc
     6361 tggcgctgtg tagtgaaggg ctgaaaattg ctccttatta agcatatcgg ccttgctctt
     6421 gagaatggct gcatgttcct cccaagcctt aacaggatct gcttcgtaga cacgacaagt
     6481 tttgaagatt tgatcccaaa ggaaatcaac tgcttcttcg tcgctcgcag catttgggaa
     6541 gactttctta gcccactcaa gtccagcagc ggctgctaca gtccagctaa ccttgttaga
     6601 ttgagttgcg atacgcatag gcttcatggc aagtcccata gctttagcag aagctgaaag
     6661 cttgtcagcg tccactccgt tcaaggcacc tggatcagat gaacgaaccc caagacggct
     6721 agccttgttc tccaaaagat agttcatctc agcaatcttg tattctggca cattgtccaa
     6781 acgctccatc ggggcatgga ggaatttctc acggttaatc acatcatctg tccactgaac
     6841 gatgacctca tgcgcaccca aggcataagc ttctttcacg attagatgtg ccaattcacg
     6901 ttgctccaca tcaatagaga gagccaaagt gtgaccaggt tgcacgttaa ttccgttcgc
     6961 aaccaacaat ttcgcatatt ttcctagatt ttctttaaaa tttggtaaaa ccattctgtt
     7021 ttctcttttc tatttttatt tttctttcaa agcagaaaag attcctgcta aagcaatagc
     7081 acctgacaag agtgctgtcg atagaagaat tgtacgatca gcaagttcta attgatattc
     7141 aaataccttc ttagccggct tatcttccgc aaatattctt agtttcatga gaaccccctt
     7201 aaaatagatt aaaataaatc actgtgactg cctgtcctca gcaaatttaa aatcaattcc
     7261 gacttgtcta ctttataaac caaaagccaa tctggctggg tatggcattc acgaactcct
     7321 tgaaaatgct tggatgccgt caatgaatga tcacgatatc tggcaggatg ttctttttct
     7381 tgaaccagaa aattcaaaac ttcttctaat aattctgcct tcaaaccacg cttcatagcc
     7441 aacttaaaat cttttttaaa ctgtttatga taacgaatct taagcacgta agtcctccat
     7501 caaatctgag actgattcaa aagactggct catattacga ttttggtcta aatccgttaa
     7561 cgcttggagc aacttccgat tttcgtctag tctaacatca aaaggtaatc cctgatattg
     7621 aattgcctga cgaaggaaaa tattaatagc tgttgtcata tccattccca gattactaaa
     7681 cacctgttgg gcctgctcct taacctcact atccagacgg atgcttatac tcatctttga
     7741 catactctca ccctctttcc atagactatt ttaacaaaaa agaaagctaa tgtaaatcta
     7801 ttggatatac gttagcctct tttaatagat tattaagcaa ttttttaaaa caactcatca
     7861 aacaaactca actggttatc ctctggcata tttccaagaa tacccatctc atccatcttt
     7921 tcaaccaagg ttgatgagag tccaccacgc ttgcgtagtt ctgttttaga gaggaattct
     7981 ccctcttcac gcgcccgcac cagttgcttg gcaacgttct ctcccagacc atccattgct
     8041 acaaatggtg ggataagggt atccccgtcg atgaggaact ctgtcgcctg actacggtag
     8101 agatctaatt taccaaactt gaaacctcgt tcccacatct cattgacaat ctcaagagtt
     8161 gtatagagat cgatttccac attagaggct tcattgttct tccgtttttc agagatttct
     8221 tccattctgc gcttgatgac ctccaagccc gcacccatgg tcttgatatc aaaagcctta
     8281 gcacgaatgg agaagtaagc acagtagtaa taaataggat ggtgaacctt gaagtaagct
     8341 acacgcaagg ccatcataac gtaggctgcc gcatgggcct tagggaacat gtacttaatt
     8401 ttcccacagg attcgatata ccactctggc accttattag ccttcatggc ttcgatatag
     8461 ccatttctct cctcttctga aatctttagc cacaaaccct tacgtacccg ttccataatg
     8521 gtaaaggcca tcttaggttc cagacccgca tgcatgaggt aaaccatgat gtcgtcccga
     8581 caaccgataa cagtcgatag gtccgctatt ccttgcttaa tcagatcctg agcattcccc
     8641 aaccaaacgt cagtaccgtg ggacagacca gacagctgaa gcaattccgc aaaggttgtc
     8701 ggatgggttt cgtctaccat tccacgtacg aaatttgttc caaactctgg aatccccaac
     8761 atacccgtag gcgttccaat ttgttcaggt gttaccccta gtacatcagt cccagaaaag
     8821 agtgccatca cgccttcgtc atccatagga attttattag ggtcaatacc agacaaatcc
     8881 tgaagttttc gaatcatggt cggatcatca tgtcccagta catcgagttt gaggacgttc
     8941 tcatcgatat cgtggaagtt aaagtgagtg gtctgccatt cagccgtgac atcatctgct
     9001 ggatactgga caggcgtaaa atcgtagaca tccatgtagt tcggaataac aacgattccc
     9061 cccgggtgtt ggcctgttgt ccgcttgaca cccgccgctc cttgagcgag gcgttctact
     9121 tctgcatcac gataaaactt gccataatct cgctcgtaac ccttgacaaa tccataggca
     9181 gtcttggcag ctaccgtacc aaccgttccc gcacggaagg catattcttc accaaagata
     9241 tcacgcacat ccaagtgggc gctaggctga tcttctcccg agaagttcaa gtcaatatca
     9301 ggaaccttat ccccatcaaa accaaggaag gtctcaaacg gaatatcctg tccgttttta
     9361 ctgagtttgt gaccacagtt tggacagtcc ttatggggca tatcaaatcc tgaaccgtac
     9421 gaaccatctg tgataaactc actgtactga cactgaccac agacatagtg aggagagaga
     9481 ggattgacct ccgtaatccc aatcatggtc gcaacgaaac tagatccgac agacccacga
     9541 gaaccaacca aataaccccg ttcattagaa cgttgcacca gcatctgcga tgccagataa
     9601 atcacagcaa atccattccc cagtatggat gttaattctt tttcaatccg caaatcaaca
     9661 atatctggca gcggatttcc ataaatctca aaagctttct tataggtcaa ctcagcaact
     9721 gtttcttcag ccttgtcgat gaaaggcgta tacaagtcac ccttaacgac ttcaacggat
     9781 tcaaatattt ctgccaaggc attggtgttt tcaataacca gtttacgagc cagttcctct
     9841 cccaaaaagg caaattcatc caacatctca ttagtcgttc gaaaatgagc ctttggaagt
     9901 ggtgctggtt gggcatgttc accatgaccg atagttcgat taatcatcgc accctgtccc
     9961 aaactacgga cgataatttc acgataaatc tcttcttccg gttcgatata gtgaacattt
    10021 cccgtagcca gaacaggctt gccaaggcgg tctccaacct ctatcaaact cttgataatg
    10081 gtctggagtt cctccatatc cttgacctgc tctttggcaa tcaagggtgc atagatagcc
    10141 ggtggcatga cctcgataaa atcataatac ttggccacct caaccgccgc atccacacct
    10201 tgagaaacga ccacgtcaaa aacttcaccc tctgaacagg ctgaacctaa aatcaagccc
    10261 tctcgatggg catctagaac cgttctcgga atccgtggca ctccttcaaa atacttggta
    10321 ttagacaagg aaaccagctt aaagatattt tttagaccta cctgattctt gacatagatg
    10381 gtcgcatgct tgatccgagc ttttttgtaa gaatctggac tgattagatc aatgttgagt
    10441 ctagctaaat cggtcacacc atgtttttct gctacctctt tgataaagat gaaaagcaga
    10501 cgaccagtcg cttccgcatc gtagttggcc atgtggtgat gttccaaggc cacaccaaaa
    10561 cgcttggtca aaggccccaa accatggcgt ttatactcag gatagaggtt tctagcaaac
    10621 tccagcgtat caataactgg ctgactaatc tttggaagat catgccgctc ataattagca
    10681 ttcataaagc caacgtcaaa ggtagcattg tgggcaacta ggaccgtatc cttgcaaaat
    10741 tcttggaatt cttgcaaaac ttgttctagt ggtttggcat ttttgacatg atcatctgta
    10801 attccagtta actctgtagt aaaggctgac aagggatgcc caggattgat aaattcatca
    10861 aattcagcaa taacattccc cttgtacatc ttagaagccg caacctgaat caagtcatta
    10921 tagatagctg aaagtcccgt cgtttccacg tcaaagacca cgtaggttgc ttctgacaag
    10981 tccatctcca cttcgttata gacgataggg acacggtcct ccacgatatt ggcttccatc
    11041 ccatagatca gctggattcc cgctttctta gccgccttat agccatgtgg aaaggactgg
    11101 acattcccat ggtccgtgat agcaaccgcc ttgtgtcccc acttagcagc tgttgcaaca
    11161 atctcttcga cctctggcaa agcatccata gtcgacatgt tagtatgagc atgaaactca
    11221 acccgacgct caccttctgg catcaaatcc ttccgctcat agtgaacaac ttcctgcaga
    11281 tcctgtacgt tcatagtcaa atcgcgtgtg aagttattca tctccacatt ccctcgaact
    11341 cggagccaag aattcttctt gatgaggtca aacttctggg cctcttcctc gtttttaacc
    11401 cacttttgca tagaaaaact tgaagtatag tccgtcattt taaagttgat taaaacacga
    11461 cctgttctag tcactttttg ctccacatca aaaacaaccc cttcaaatac cagacgattt
    11521 tcctctgtcg tcacttcgat cataggagta atctccgcct tatccagctt gggtttagct
    11581 gcagcttttt tcgcttgaaa atcaaagact ggtttctctt ccgctggagg aggtgccatc
    11641 tgctccagtt gttccatagc acggagcgct tcctcattgg cagcttgaac aatctgctca
    11701 ttttcagcat gaaaggcctc ttcctgctct tgggtcagga catcattctt ctcgacttga
    11761 cagttaaaag ttggaaaacc aaacttttca agttgtttgg ctaaattagg aagatgattc
    11821 ttcttaaaat gttccttatc aatcgcttca gatccttcaa taaatagctg attaccctca
    11881 gcacgaactt gcaaattttg ataaagggac ttaaaacctt gactagcaca tggaccttca
    11941 gagaaagcct ccctatagta ggactgcaag agctgatttg aaaattcttg agaccgagcc
    12001 ttaatttcaa aaacagcttt attgcctgtc ttagaaaatt cttcgctcaa acctttcttt
    12061 aattctaaaa agatttcaat cggtaaaata ttagaaaata cgaaatgaaa ctcccatacc
    12121 ttactaattt tatgaaccac aactcgctca atattggcct gtgctaaagc aggagcctgt
    12181 ctcatttcag caggcatccc caattgattc atcaaaattt caaaactatt tgacattcat
    12241 tttcctcaca ttattcttct actattttac catatttaga ggtattttct aaagacaaaa
    12301 ggaagccact aagtgacttc cttctagagt gaggacggat tagtcttcac ctttattttt
    12361 cttaataatt tcttcttgta ctgacttagg tacatcttcg tagtggtcaa ataccatcat
    12421 gaatgtacca cgtccttgag atgcagaacg aagaactgtt gcgtaaccga acatttcagc
    12481 aagtggaacg taagcacgaa cgatttggct gttaccgtgt gcttccatac catctacacg
    12541 tccacgacga gcagttacgt gacccataac atcaccaagg ttttcttctg gaacagtgat
    12601 tgttacaagc atcattggtt caaggatagc tggttgtgct gatttagcag cttctttaag
    12661 ggaaagtgaa gccgcaatct tgaaggcagt ttcagatgag tcgacatcgt gatatgaacc
    12721 atcataaagc ttagctttaa cgtcaaccat tgggtaacct gcaagaacac cgttagccat
    12781 agattctacc aaaccttttt caaccgctgg gataaattca cgaggaacca caccaccgac
    12841 gattgcgttt tcgaattcga atcctttacc ttcttcgttt ggagtaaatt caatccatac
    12901 atcaccgaat tgacctttac caccagactg acgtttgaag aatccacgtg cttgagtaga
    12961 agcgcggaat gtttcacggt aagatacttg aggagcacct acgttcgctt caactttgaa
    13021 ctcacgacgc atacgatcaa caaggacgtc aaggtgaagt tcacccatac ctgagataac
    13081 tgtttcacca gtttcaacgt ttgtttcaac gcggaatgtt ggatcttctt cagccaattt
    13141 ttgaagggcg atacccatct tgtcttggtc agctttagat tttggctcaa ccatcaattg
    13201 gataactggt tctggaacgt tgattgactc aaggatgatt ttagcttttt catctgtcaa
    13261 tgagtcacca gttgtagtat ctttcaaacc aacggcagca gcgatatcac ctgagtaaac
    13321 agtgtcgatt tcttgacggc tgttagcgtg catttgaagg atacgtccga tacgttcacg
    13381 tttaccttta gaagtattca atacgtatga acctgattga agaacacctg agtaaacacg
    13441 gaagaatgtc aaacgaccta cgaatgggtc agtcatgatc ttgaaggcaa gagctgcaaa
    13501 tggctcttcg tcagatgctg gacgaatttc ttcagcgtct gtatctgggt taataccttt
    13561 gattgctggg atgtcaagtg ggcttggaag gtagtcgata accgcatcaa gcatcaattg
    13621 aacacctttg tttttgaagg ctgaaccaca caatactggg aagaattcaa cgttgatagt
    13681 cgctttacgg ataccagctt tcaattcttc gttagtgatt tcttcacctt cgaggtattt
    13741 catcatcaat tcttcgtcag tttcagcaac tgcttcaatc aatttttcac ggtattcttg
    13801 agcttggtca aggtattcag ctgggatgtc ttcttcaagg atatccgtac caaggtcgtt
    13861 agtatagatt tcagctttca tcttgatcaa gtcaatgata ccacggaagt catcttcaga
    13921 accgattggc aattggattg ggtgtgcatt tgcttgaaga cgatcgtgaa gtgtgcttac
    13981 agagtaaagg aagtcagcac cgattttgtc cattttgttg gcaaatacga tacgtggaac
    14041 tccgtactca gttgcttgac gccaaactgt ttcagtttga ggctcaacac ctgattgtga
    14101 gtcaagaacg gtaaccgcac catccaatac acgaagagaa cgttgtactt cgattgtgaa
    14161 gtccacgtgt cctggtgtgt cgatgatgtt tacgcggtgg ttgttccatt gagctgtcgt
    14221 cgcagcagat gtgatcgtga taccacgttc ttgctcttgc tccatccagt ccatttgtga
    14281 cgcaccttcg tgagtttcac cgattttgtg gattttacca gtgtagtaaa gaatacgctc
    14341 agtagttgtt gttttaccgg catcgacgtg agccatgata ccgatattac gagttttttc
    14401 aagtgaaaat tcgcgtgcca tgaggtttgt ttctcctatt tatttttgat ttctattcta
    14461 ttataacacg attttaataa aaacggatag gcaggaccta cccgttctca atgttttcat
    14521 gctattgttg gtttcaactt acgagatggt aagctgagtt atagctaata ctaatcgatt
    14581 tagctaattt gaacccgggc taaagttagt tagccgatgt gagctggaac gggatgctgc
    14641 gtgaaaaaga taaaactcct tgtattcgtc gaatactgcg tcagtttcct atttttacct
    14701 tgcatcctta ccgttcctag catcatgatt tagttgagct caagctaaaa gtctggagaa
    14761 aaagatgaat ctcattggtt gcaaccgcaa cctgcttcga ttccctattt tctctgggac
    14821 tttcttaacg ctttcgcatc ctatcttacc aacggaagtg tgcgaatgca cggttagctt
    14881 cagccatacg gtgagtatct tcacgtttct taactgctgc accagtgttg ttagcagcat
    14941 ccaagatttc ttttgcaaga cggtcttgca ttgtgtgttc accacgaaga cgagcgattg
    15001 ttaccaacca acgaagtcca agtgttgtac gacgttctgg acgaacttca actgggactt
    15061 ggtagttaga accaccaaca cgacgtgcac gtacttcaag tacaggcatg atgttttcca
    15121 tagctgtttc aaatacttca agtgcatcgt tgccagtagc ttctttgatt tgctcaaaag
    15181 caccgtaaac gattgaagca gcagtaccac gtttaccatc aagcataacg cggttgataa
    15241 gacgagtaac tagttgtgaa ttgtatagcg gatctggcaa tacgtcacgt tttggagctc
    15301 tatttttacg actcatttct ctttatcccc tttccttatg cttttggacg tttagtaccg
    15361 tatttagaac ggccttgttt acgatcgtta acacctgcag tatcaagtgc accacggacg
    15421 atatggtaac gtacccctgg aaggtctttt acacgtccac cgcgaagaag caccacgctg
    15481 tgctcttgca agttgtgtcc gatacctggg atgtaggcag taacttcgat aaggttgctc
    15541 aaacgtacac gagcgaattt acgaagggct gagttaggtt ttttaggtgt cattgttcca
    15601 acacgagttg caacaccacg tttttgtggt gaagaaacgt ttgtttgaac ttttttatga
    15661 ctattgtaac caacgttcaa agctggtgat ttagattttt ctacttttga tttacgcggt
    15721 ttgcgaacca attggttaat tgtaggcatc tacattctcc tgtgtttttt tatttttggt
    15781 gatgatacac ttggtgacag atatcatctg tgtgtacttt tgcaacattt gtcagcacgt
    15841 ccctgtacac tcttgagaga ccaaaagtaa aaagtaccgt ctattattgt aacaaaattt
    15901 tcccttgctt gtcaagatat ttttcttttt tattggtaat gttagagatc aatttctgaa
    15961 taaaaatagc tgagaacaca tcatcttcct aataacacgg cttggcttac gtgacatttt
    16021 tatctccgtc atttatactg atatataaaa tagtacggaa aacttctcaa aagtaagaat
    16081 ttctttcaca aatcaaagaa aacgcttgca tattgtttta ctacatgcta tactgatata
    16141 gaaatttatt gattggagaa tcattatgag aaaaacacca tctcacactg agaaaaaaat
    16201 ggtctacagc atccgttccc tcaaaaatgg aactggttct gtccttatcg gcacaagcct
    16261 tgtcttgctt gccatggcta caccaactat ctcatccgac gaaagtacac caaccactaa
    16321 cgaacccaac aacagaaata caaccaccct tgcccaacct cttactgata cagcagctgg
    16381 ctctggtaag aacgaaagtg atatttcttc acctggaaat gcaaacgctt ccctagagaa
    16441 aacagaagaa aaacctgctg caagcccagc cgatccagca ccacaaactg gacaagatcg
    16501 ttcaagtgag ccaactactt ctactagtcc agtaacaact gaaactaagg cagaagagcc
    16561 catcgaagat aactacttcc gtatccatgt caaaaaactt cctgaagaaa acaaggatgc
    16621 tcaaggacta tggacttggg acgatgttga aaaaccatct gaaaactggc caaacggagc
    16681 tttgtccttc aaggatgcca agaaagatga ctacggctat tacctagatg tcaaattaaa
    16741 gggagaacaa gccaagaaaa ttagcttcct catcaacaat acagctggaa aaaatctaac
    16801 cggcgataaa tctgtagaaa aactagttcc aaaaatgaac gaagcttggt tagaccaaga
    16861 ttacaaggtt ttctcttacg agccacagcc tgcaggaact gttcgcgtca actactaccg
    16921 cacagatggc aactatgaca agaaatctct ctggtactgg ggagatgtga aaaatccaag
    16981 tagcgctcaa tggcctgacg gaacagactt tacggctaca ggcaaatatg gccgctatat
    17041 cgacattcct cttaatgaag ccgcaagaga atttggattt ttattactag atgagagcaa
    17101 acaaggagac gacgtgaaaa tccgtaaaga aaattataag ttcacagatt tgaaaaatca
    17161 tagccaaatt ttcctaaaag acgatgatga atcgatttac acaaatccat actatgtcca
    17221 tgatatccgt atgacaggag cccaacacgt aggcacttct agcattgaaa gtagcttttc
    17281 aacacttgtc ggtgctaaaa aagaagatat cctcaaacac tccaacatca ctaatcacct
    17341 aggaaacaag gtaactatta ccgatgttgc aatcgatgaa gctggtaaga aagtgaccta
    17401 cagcggagat ttctctgaca caaaacatcc ttatactgtt agctacaatt ccgaccaatt
    17461 cactaccaaa acaagctggc gcctgaaaga tgagacatac agctatgatg gcaaactggg
    17521 agctgaccta aaagaagaag gaaaacaagt tgatttgacc ctttggtcac caagtgctga
    17581 taaggtttct gttgttgtct acgacaagaa tgaccctgac aaagtagttg gaactgtcgc
    17641 tcttgaaaaa ggggaaagag gaacttggaa acaaactcta gacagcacaa acaaactcgg
    17701 aatcacagat ttcactggct actattatca ataccaaatc gagcgtcaag gtaaaactgt
    17761 tcttgcactc gatccttacg ctaaatctct tgctgcttgg aatagcgacg attccaagat
    17821 tgacgatgcc cataaagtgg ctaaagccgc ctttgtagat ccagctaaac tcggacctca
    17881 agacttgact tatggtaaga ttcacaattt caagactcgt gaagacgccg ttatctacga
    17941 agctcatgtg cgtgatttca cttcagatcc tgccattgca aaagacttga ccaaaccatt
    18001 tgggactttt gaagccttca ttgaaaaact agactatctc aaagacttgg gtgtaaccca
    18061 tatccagctc cttccagtct tgtcttacta ctttgtcaat gaattgaaaa accatgaacg
    18121 cttgtctgac tacgcttcaa gcaacagcaa ctacaactgg ggatatgacc ctcaaaacta
    18181 cttctccttg actggtatgt actcaagcga tcctaagaat ccagaaaaac gaatcgcaga
    18241 atttaaaaac ctcatcaacg aaatccacaa acgtggtatg ggagctatcc tagatgtcgt
    18301 ttataaccac acagccaaag tcgatctctt tgaagatttg gaaccaaact actaccactt
    18361 tatggatgcc gatggcacac ctcgaactag ctttggtggt ggacgcttgg ggacaaccca
    18421 ccatatgacc aaacggctcc taattgactc tatcaaatac ctagttgata cctacaaagt
    18481 ggatggcttc cgtttcgata tgatgggaga ccatgacgcc acttctatcg aagaagctta
    18541 caaggctgca cgcgccctca atccaaacct catcatgctt ggtgaaggtt ggagaaccta
    18601 tgccggtgat gaaaacatgc ctactaaagc tgctgaccaa gattggatga aacataccga
    18661 tactgtcgct gtcttttcag atgacatccg taacaacctc aaatctggtt atccaaacga
    18721 aggtcaacct gcctttatca caggtggcaa gcgtgatgtc aacaccatct ttaaaaatct
    18781 cattgctcaa ccaactaact ttgaagctga cagccctgga gatgtcatcc aatacatcgc
    18841 agcccatgat aacttgaccc tctttgacat cattgcccag tctatcaaaa aagacccaag
    18901 caaggctgag aactatgctg aaatccaccg tcgtttacga cttggaaatc tcatggtctt
    18961 gacagctcaa ggaactccat ttatccactc cggtcaggaa tatggacgta ctaaacaatt
    19021 ccgtgaccca gcctacaaga ctccagtagc agaggataag gttccaaaca aatctcactt
    19081 gttgcgtgat aaggacggca acccatttga ctatccttac ttcatccatg actcttacga
    19141 ttctagtgat gcagtcaaca agtttgactg gactaaggct acagatggta aagcttatcc
    19201 tgaaaatgtc aagagccgtg actatatgaa aggtttgatt gcccttcgtc aatctacaga
    19261 tgccttccga cttaagagtc ttcaagatat caaagaccgt gtccacctca tcactgtccc
    19321 aggccaaaat ggtgtggaaa aagaggatgt agtgattggc tatcaaatca ctgctccaaa
    19381 cggcgatatc tacgcagtct ttgtcaatgc ggatgaaaaa gctcgcgaat ttaatttggg
    19441 aactgccttt gcacatctaa gaaatgcgga agttttggca gatgaaaacc aagcaggatc
    19501 agtcggaatt gccaacccga aaggacttga atggactgaa aaaggcttga aattgaatgc
    19561 ccttacagct actgttcttc gagtctctca aaatggaact agccatgagt caactgcaga
    19621 agagaaacca gactcaaccc cttccaagcc tgaacatcaa aatgaagctt ctcaccctga
    19681 acatcaagat ccagctccag aagctagacc tgattctact aaaccagata ccaaagtagc
    19741 tgatgcggaa aataaaccta gccaagctac agctgattca caagctgaac aaccagcaca
    19801 agaagcacaa gcatcatctg taaaagaagc ggttcgaaac gaatcggtag aaaactctag
    19861 caagaaaaat atacctgcaa ccccagatag acaagctgaa cttccaaata caggaattaa
    19921 aaacgaaaac aaactcctat ttgcaggaat cagcctcctt gcgctccttg gtctcggttt
    19981 cttactaaaa aataaaaaag agaactaaac tagccctcct atagaaaaat cccccaagca
    20041 ttatagctcg ggggattaat tttcgtacaa tatttgttgt cctaataaac ttgattagga
    20101 ttttttatta agcctctttc atagcaaaat aagctcgtac tttgggtgca acttgtgttc
    20161 cgaagagttc aatagctctc agaacctggt catgaggcat agaaccaagc ggtagatgaa
    20221 gcatgaagcg gtccaatcct aaatcctcta tcatgcgaat caatttttcg gccacctgat
    20281 ctggattgcc aacaaacatg gcgccatttg gccctacctg ctccaaatat tgctcataac
    20341 gcaattcctg ccagtgcgga cggtctttgg aaatagcatc caccacttgc ttagtcggat
    20401 ggaaataatc tttcaccgcc tgctcaccat cttccgcaat ccacccccaa gaatgggctc
    20461 ccactttcaa gtctttgtca gcatggcccg cttcgcttcc aatctcacga taagcctgaa
    20521 tcaacttttt aaaataacgt ggattaccac caataatagc atatacaatc ggtagaccag
    20581 cctgagcaat cttcactgtt gattcgacat gaccacctgt agctatccac aagggcaatt
    20641 tgtcctgaac tggacgagga taaacttctt taccagcaat cgtttgagtc aatcgacctt
    20701 gccagtctaa cttggtcttt tcattgacta actgaagcaa gtctaatttc tcatcaaaaa
    20761 gagagtcgta gtctttcaag tcataaccaa acagagggaa agattccgtg aaagagcccc
    20821 ttccagccat aatctccgat cgtccatttg acaaagcatc gatagtggca tactgttgga
    20881 acaaacgaat cggatccatg cttgacagaa tgctgactgc actggtcaaa cggattttct
    20941 tggtattgac tgccccagcg gccaggacaa tctctggggc tgatactgca aaatccgccc
    21001 gatggtgctc gccaatccca tatacgtcca aaccaacctt gtcagccagc tcaatctctg
    21061 ccaccaactg gcgaatgcgt tcagcatgac tgtaagtttg tccagtccct tcaagctccg
    21121 ttatttcccc aaatgttgaa attcccaatt ctaccattgt gattctcctt gtctatctct
    21181 gtccttcaat ttgaaaaatt attctaacac gaatcttaag tacaagcaac caatttgctc
    21241 attagaaaaa gcctagataa ctagactttt ttagcttatt ctaccgttac tgatttagca
    21301 aggttacgtg gtttatccac atcgaggcca cggtggaggg ttgcaaagta agcaaccaat
    21361 tgcgtcggta cgaccattga aattggtgag aggtaagggt gtacagtcgt aaggacgata
    21421 tcgtcggtat ctttagcaac attttcttct gcgatagtga ggaccttagc accacgggct
    21481 gccacctctt ggatatttcc acgagtgtgg ttggcaagga ctggatctga caagagagcc
    21541 aagacaggtg ttccttcttc aatcaaggca atggttccgt gcttgagttc tcctgcagca
    21601 aagccttcac actggatata agaaatctct ttgagtttga gacttgcttc catggctacg
    21661 tagtaatctt gaccacgtcc gatgtaaaag gcgttacgag ttgtttcaag aagttcacga
    21721 accttgactt caatggtttc tttctctgaa agagttgatt cgatagactg agctacgatt
    21781 gacaattcat gaaccagatc aaagacttgc gctttagcat taccatttgc ttctccgact
    21841 gcttttgcaa ggaaggcaag ggctgcgatt tgcgctgtat aggctttagt tgatgccaca
    21901 gcaatttcag gacctgcgtg aaggagcatg gtatagttgg cttcacgtga gagggttgaa
    21961 cctggaacat ttgtcactgt taagcttgga attcccattt cattagcctt gaccaaaact
    22021 tgacgactat ccgctgtttc accagattgg ctgataaaga tgaagagtgg tttcttgctg
    22081 agaagtggca taccgtagcc ccactcagat gagattccaa gttcaactgg tgtatctgtc
    22141 aattcttcca acattttctt agaagcaaat cctgcatggt aagatgttcc agctgcaagg
    22201 atgtagatgc ggtctgcgtc ttgaacagcc ttaatgatat ctgggtctac gacaacttga
    22261 ccagcctcat ctgtgtaggc ttggatgagt ttccgcataa cagttggttg ctcgtcaatt
    22321 tccttgagca tgtagtaagg gtaagttccc ttaccgatat ctgacaagtc aagttcagca
    22381 gtgtagctag tacgctcacg acgatttcca tcatagtctt gaacttccac actatcagcc
    22441 ttgacgatta ccaactcttg gtcatggatt tccatgtatt ggttagtttc acgaatcata
    22501 gccatggcgt ctgagcagac catgttatag ccttctccaa gaccaatcaa aagtggtgat
    22561 ttatttttag ctacgtagat gacttcagga tcttgtgagt caaccaaggc aaaggcataa
    22621 gaaccacgga tgatgtgaag ggcttttttg aaggcttcaa gaactgagag cccttcttct
    22681 tccgcaaatt ttccaatcaa atgaacggct atttcagtat ctgtctgccc cttgaagtgg
    22741 tgacctgcaa ggtattcttc cttgatttca aggtagttct caatcacccc attatgcacc
    22801 aagacaaaac gttctgtctc agagcggtgt gggtgagcat tgtcctcagt tggttttccg
    22861 tgagtagccc aacgagtatg tccgatacca gttgttccct caacaccagc tgtcttggca
    22921 gacaattctg caatacgacc aaccgccttc accaaatggt tatcagcacc atctaggaca
    22981 aaaattcccg cagaatcata gccacggtat tcaagctttt caagcccttg aatcaaaata
    23041 tcagttgcat ttgtgtttcc aacaacacca acaattccac acatagtata tacgacacag
    23101 gcaagctgtg ctttctcctt aaaattggta tagtctaatt catcttttat agaatcagca
    23161 aaaacagtat atacttgttt ctttcacttg tcaagagtaa aaattggtat agttcaaatt
    23221 aagctcctgt aagcataaaa actctgaccg attgggataa tcagtcagag tcctttttaa
    23281 aatccattat tatcgcttaa ttctttgaac cagtggcctg atttcttcag acgacgttct
    23341 tgcgtttcca agtctaattc gaccaaacca tagcgatttt tatagctgtt gagccatgac
    23401 cagcagtcaa taaaggtcca aatcaagtag cccttacagt tggcaccatc ttcaatggca
    23461 cggtgaagtt cacgaagatg accttttaca aagtcaatac ggtaatcatc ttgaatcatt
    23521 ccatcttgac ggaatttttc ttccccttca acacccatac cattctcagt caacatccac
    23581 tcaatattgc cataattttc cttgatattt tgggcgatgt cataaatccc ttgctcataa
    23641 atctcccaac cacggtgaga attgatttta cgtccaggca tcacataagg ctcgtaaaaa
    23701 tgttctggta agagtggact ctctggatgc ttagcaaatc gaggagccat aacacgcaaa
    23761 ggttgatagt agttcacacc aaggaagtcc accgtattat cacgaatgag ttccaactct
    23821 tcctctgtag catcaggtaa aagaccgtgt tcatgcaaga tttctaccaa ctcctgtgga
    23881 taagtcccca agacagatgg atctaagaaa gattgggcct gaaaaagggc cgcaatacga
    23941 gctgccttga catcagcagg atgctggcta cgtggataag ccggtgtcaa gttgaggaca
    24001 atcccaatct tggaatcagg caaaagttca tggcaagcct taacagcccg gctgctggcc
    24061 aattgtgtat gataggctac cttaacagct gcctctgcat ccaccttatg tggataatgg
    24121 gcatcataaa aataaccaaa ttctacagga acgatgggct cgttaaaggt aatccattga
    24181 tccactaaat ctccataagt ctcaaaacaa aaacgagcat agtcttcata ggctgagact
    24241 gtcgccttat tttcccaacc atcaccatcc tcttgaaggg caaaaggtaa atcaaaatga
    24301 tagagattga ctaacagacg aattccttta gccttaatag cctcaaagac cttacgataa
    24361 aaatccacac cttgagggtt gacttttcca cagccttgtg gaaaaatccg tgaccactga
    24421 atagaagtcc gaaaggctgt gtgaccagtc tctaacaaaa gctcaatatc ccgctcccaa
    24481 ttttcataaa aagtcgatgt cttatctgaa ccaatcccat tatagtaacg atttggctcc
    24541 acttggaacc agtaatccca gagattgtct cccttaccgt caccagctac acgtccttct
    24601 gtctgcggtc cagaagtaga ggatccccag acaaaatcct ttggaaatct tagcatacat
    24661 ttacctcttt atctactcat ttctcccatt atacagaaaa aacaaggtaa aaactagtta
    24721 cattttttcc ttgtttttct tctgattata gtttttattt cttgcttagg atttcaagcg
    24781 tttcaagcac gttatctgca tgaacctcaa tggtgtcacc agttgccttg atcttaactt
    24841 ctacaatgcc atcggccgct tttttaccaa cagtgatacg gattggaaga ccaatcaagt
    24901 cactatcgct aaatttaaca ccgacacgtt cgttacggtc atctgtcaag acttcataac
    24961 cagctcccat caagcttgct tcaagttttt ctgtcaaggc ttgcgcttct tcatccttga
    25021 cattgacagt aatcaaatgc acatcaaatg gtgccaattc tttagggaaa ttgattcccc
    25081 aagcgtaacg gtattcacct tttggcgttt tgttaacaaa gaggcgagcg tgttgctcca
    25141 tcactgctga aagaagacgg ctgacaccga taccgtaaca tcccatgatg attggcacag
    25201 cacgaccatt ttcatccaag acatctgctc ccatgcttgc tgaatagcga gttccgagtt
    25261 tgaaaatatg accgatctca ataccacgcg caaaattaag gacaccttgt ccatctgggg
    25321 aaatttcacc ctcacgaact tcacggatat ccacatattc tgaagtaaaa tcacggcctg
    25381 ggttcacacc agtcaagtgg tagccatctt cgttagcacc gacaactgca ttgcgaacat
    25441 cttgcacctt acgatctgca ataattttaa tattctctgg caaaccaact ggtccaagtg
    25501 aaccaaatcc tgcttgaaca acattcgcca cttcttcttc gctagcaacg tcaaagaaat
    25561 ctgctcctaa gtggtttttc aacttgactt cgttgagttg gtcatttcca actagaaggg
    25621 ctgcaacaag ctcaccatct gcaatgtaga agagggtttt aatcgtttgt tcttctggaa
    25681 cattgaggaa agctgcaact tcatcaattg atttaacatc tggcgttgca acacgagtaa
    25741 cttcttcttc agcgacaaca cggttgcttg gtttgtactc gtttgttgcc atttctaagt
    25801 tagctgcata gctagactca cttgagtaag caatggtatc ttcaccagag actatccatt
    25861 tgagcaattc tgccttgatt tcttcttgca cttctgcagg aatttcgtca aatgaggcaa
    25921 ctgacttgtc caagacaacc cagcggtcaa ggtctgtacg agcagatgta atggccataa
    25981 attcttggct atccttacca cccatggctc caccgtcacc aataatagcc ttgaagtcta
    26041 aaccactacg agtgaaaata cgctcatagg ctgctttgta ctcatcataa acactatcca
    26101 aactatcata gttagcgtgg aaactataag catctttcat gataaactca cgtgtacgaa
    26161 gaagtccatt acgcgggcgt ttttcatcac gatacttggg ctgaatttga taaaggttga
    26221 gtggcaattg cttgtaagat ttaacagaat cacggacaat agctgtaaag gtttcttcgt
    26281 gagttggacc taagataaag tctgattttt cacggttttt tagtttgtaa aggtcttcac
    26341 cgtaagtttc ataacgacct gattcacgcc acaattctgc actaagaagg gctggagcca
    26401 acatctcaac agcaccaatc ttttcgaatt cttggcgcat gatgttttta gctttttcaa
    26461 tcacacggtt ggcaagtggt agataagaat aaacacctgc tgaaacttgg cgaacataac
    26521 cagcacgcaa cataagagca tggctgataa cttgagcatc gcttggcatt tcgcgaagcg
    26581 ttgggatagg cattttactt tgtttcataa tattcctcga ttatctaaaa aagagtcgca
    26641 taatgtcatt ccaagtcaca gcaatcatca agacaaccat gatgaccact ccggccaagg
    26701 tgacataggt ttcaatttct tgtttcaatg gtttgcggcg gatggcttct aggatattga
    26761 gcacaatctt accaccatcc aaggctggaa tcggaataag attaaaaatc ccaatattga
    26821 tggaaatcat tgccaagaag tacaagatat tttcaattcc atttttagca gcatcactac
    26881 ttgccttaaa gatagcaaca ggtccaccca acttgttcaa atccggttgg aaaatcagat
    26941 ttttcagagc tgagagaatt cggagagctg agtcagcagc agttgtaaaa ccacctacaa
    27001 acatggatag aaaatctgac ttaacccccg gttgaacacc tagaaggtaa cgaccttgac
    27061 tatcttcggg tgtaacagtg acttgtttgt cactcccctt ttcagaaata gtcacatcca
    27121 aagtcggtgc cgtcttatct ttggtttctg tttccacagc ttggatcaag ctttcccagt
    27181 tgctaacctc atgtgagcca atcttggtaa tttgtgccgt ttctggtact cctaccttgg
    27241 ccaaggcacc ttggggcatg atatggaact gattggtatc aacatctctg acaccaccct
    27301 gcataaagat taaaacccaa aaaacaacga cacctaagat aaaattgttc ataggacctg
    27361 caaaattggt aatcagtttg ccccagatag tcgcattttg atattgaaca tctaaaggtg
    27421 caatccgaac ctcagtacca tctgcttcca caaccgttgc atcgtgatcc actgcaaatg
    27481 ttttttcttc ttccagaacc aatcctttga taaagagctt gtcttcaaaa tcaaactggg
    27541 tcacctgcat agggagggct gtttgatcca attttttacc tgagagattg atgcgtttaa
    27601 ccttaccatc atcagcaagt gtcaaactaa caggcgttcc tgtcttgatt tcagttgtat
    27661 catcacccca accggccatg cggacatagc cacccagagg caagattcga atggtatagg
    27721 ccgttccatc cttgccaatg tgagcaaaga ttttaggtcc cataccgatg gcaaattcac
    27781 gtactaaaat ccctgatttc ttggcaaagt agaagtgccc gaactcgtgc accactacaa
    27841 taatcccgaa aaccagaata aaggttaaaa ttccgagcat agcgtttcct ccgtcttttg
    27901 attaaaagag tccaaataag tgcatgattg gaaatacaag caacatacta tcgaaacgat
    27961 ccaaaacacc accatgtcca gggataaatt tcccagaatc cttaacacca aaatgacgtt
    28021 tgatcgaact ttctagtaaa tcaccaaatt gtccagcaat gctaaagaaa atagcaaaga
    28081 ctgacatctt gtaaattcca tatggaagag caactgtact gtcaactatc ataaagataa
    28141 tggttactaa aattgctcct aaaataccac ccaaggcacc ctcaagggtt ttattaggcg
    28201 atacccttgg tgctaacttt cgtttcccat agttcatccc aacaagatag gcaccactgt
    28261 ctgtcgccca gacgatacac aaggctaaga gagccttgtc caaacctgca acacgagcat
    28321 ctagtaaagc attaaatcca aagcccacgt agaagctcat agcaagaggg aaaaccgcat
    28381 cctcaatcgt ataagacttg ctaaaaacgg tcgttcctaa catgattgaa atcaaaacac
    28441 tataggcaac cacattccca tcaactggca aaaaagtcag gtaattctcc aagggaatgg
    28501 ttaatgcaaa ggttgcaaag agggtcaaga ggccctccat cgtcatggtc gctagacctc
    28561 ttatcttcaa aagttcatgc atggctagca tggctatgat tccgattgct atctgaagca
    28621 agaggccccc aatcattaaa attggtagga aaatagccag ggcaatccct gcaaacaagg
    28681 ttcttttctg taaatcctgg gtcatatttc ctcctaaact cctccaaatc ggcgatgacg
    28741 acgattatag gcaagaatag cttcctgcaa ggccgcttcg tcaaaatcag gccataaggt
    28801 gtccgtaaaa taaagctcac tataggctcc ctgccatgga aggaaattgc tcaaacgcaa
    28861 ttctccacta gtacggataa tcaagtctgg gtctcgtaag tccttaggca aatgctgggt
    28921 aaagagatag ttaccaatca attcctctgt gatgtcacct ggattgattt tggcatctaa
    28981 aacatcctgg gaaatcaact taagcgcctg tgtaatctca gcacgtccac catagttaag
    29041 agcaaaatta agaatcaatc ctgtgttgtt cttagtcaat tcctcagcct tggttaaagc
    29101 ttcaaaggtt tgcttaggca ggcggtctgt ctccccaatc atttgaatct taacattatt
    29161 cgcatgtagt tccgggacat aattatcata aaactctact ggcaagttca tgataaactt
    29221 gacttcctga tctggacggg tccagttttc cgtagaaaaa gcatagaccg taataacctt
    29281 gacgcccagt ttgttggctg ccttggtcac ggtttgcaat gcttccatgc ccgccttatg
    29341 tccaaaaact cgcggttgca tacgtttttt agcccaacgg ccattgccat ccatgatgat
    29401 gccgatatga gcaggaacct gtgtcggaac ctctacttcc acagccttat ctttcttaaa
    29461 aaatccaaac atgatcttat tcctattcaa aaatctatcg ttttattata ccatatttcc
    29521 ccattttctt ctatcactaa gctatttatt ctcaggcacc aagcccattt ttcaaaaaaa
    29581 taagccgcct gattgggcga ctttattttt atagggagat tattatgaaa aagttttagg
    29641 agtttaagtt aaggtcttct taacttatga acttagtgta cactccctag cttaaagttt
    29701 ccttaagtat ttttaaaaat caaatttttc catttctcct gccaattttt cttggataaa
    29761 cgtgtttgat agagttccat tcggtcttca ttttctaaga aatgaggagt tggacgaact
    29821 tgaaaattca aaatatcctc caaaccataa ggtgcataga gttcaaaatc tgattcttca
    29881 ttcaagcaca gtccaactgt cgtacaccgt tctggatact tactcatagc atcacgagaa
    29941 ctggtatagg aagcagtgtg aggactgtgc tgatgcatat agacctgatt tttcaattcc
    30001 cactggtact gaggaaaatc ctctcgcagc tttttctcca gtaataaggt ttcctcataa
    30061 gaaaaatctg gatcaaagaa aatcacatct atatctgttt catgatcaaa aggggatttg
    30121 tctgacaaaa gattccaaat gaaatttctg acagaacctg ctgccaacca cgagtctttc
    30181 agaccaaggt ctcggatgat cgtcagaatg gccatcatat ctggattttc tctaaaagcc
    30241 tctaagattt cttgcttatt tttcactgta ttcataacct aagtgctcat atgccttagc
    30301 agtcgccacc cgtccagacc gtgtccgcat gataaaacct ttttgaatca agtaaggctc
    30361 atacatatct tcaactgtct cacgctcttc ggcgatattc acagaaagag ttcctagacc
    30421 aacaggtcct ccactgtaca tctcaatcat ggtacgaagg attttttgat ccacatagtc
    30481 caaaccttca tggtcaacat ccagcatagt caaagcctta tcggtaataa tatcatcaat
    30541 taccccattc cccattatct gggcaaaatc gcgcacgcgc ttgaggagac gattggcaat
    30601 acgaggggtc ccacgactac gtagggccaa ctcagatgct gcctcatgag tgatttccat
    30661 ctcaaaaata tctgccgtcc gctcgacaat ttctgtcaag tcagcatggg cataatactc
    30721 catatggcct gtaatcccaa aacgtgcccg tagcggattg gagagcatac cagcccgagt
    30781 cgtcgcacca atcaaggtaa aaggtggtaa ctccaaatga acactacgac taccctcacc
    30841 agccccaatc ataatatcta tgtagaagtc ctccatagca ctataaagca cctcttccac
    30901 tgacattggc aaacgatgga tctcgtcaat aaagaggaca tctccaggct ctaactcatt
    30961 caaaatcgct accaaatcac ccgctttttc gataacagga ccagacgttt gcttgagatt
    31021 gactcccagt tcattggcaa tgacaaaagc catggttgtt ttcccaagcc ctggagggcc
    31081 aaataagagc acatgatcca gcgcttcatc ccgcatttta gcggcttcga taaagatctg
    31141 aagctgatcc ttaaccttat cctgaccaat atattcacgt aaatactgag gacggagcgt
    31201 gcgttctact aactcctcat cacccatcat ctcattatct aaaattctac tcatggctct
    31261 attatatcaa aaaaacaagc cacaaacaaa aaagccacct gattgggtga ctcctaagtt
    31321 tagcacttat gtggtataat attatacgac actgctacac cgcctacgaa aggaggtgag
    31381 atagcccatg atggaattag tactcaaaac tatcatcgga ccaattgtgg tcggtgtcgt
    31441 tcttcgtata gtcgataaat ggctaaacaa ggacaaatag tgtcaaaaaa gacctcaagc
    31501 ttatttggtc gtgagcttgg ggtcttttct agcctatgat atagaactag tactcaattc
    31561 ctttttatta tcacatagtt cacgaatttt gtcaaaggtt tacattttct tcaaccgctg
    31621 tacgacaaga cggttaagat taagagaacg ttagggattc tatcaatttc atagaaattt
    31681 tgatttcgta aacgaagaga caatcttaca tgtcacttct catttaatac gccactacta
    31741 gacaagcaaa atcattatta cagtagttcc agttcttcaa ttaacagtca cttacaatca
    31801 aattgagttt gaactagctg aagcgaccac agacctattt cttagtcata ttcgctaaaa
    31861 aagtccccgc caattccccg accaaaa
//
LOCUS       DAVKWS010000021        30304 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKWS010000021 DAVKWS010000000
VERSION     DAVKWS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.021"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            737..1264
                     /locus_tag="VW852_001020"
     CDS             737..1264
                     /locus_tag="VW852_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272184.1"
                     /translation="MLIVSAIPSVSAVYASEQVSQIETNMELQPVTSLTEEQINTLAN
                     EIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGITVNQMGALLDTAIALA
                     LGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALEFAMNLLSPGTYLAQL
                     WDKNDAIPNNGRINF"
     gene            complement(2309..4567)
                     /gene="glgP"
                     /locus_tag="VW852_001021"
     CDS             complement(2309..4567)
                     /gene="glgP"
                     /locus_tag="VW852_001021"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV0272185.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(4593..6110)
                     /gene="malQ"
                     /locus_tag="VW852_001022"
     CDS             complement(4593..6110)
                     /gene="malQ"
                     /locus_tag="VW852_001022"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV0272186.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            6660..7931
                     /locus_tag="VW852_001023"
     CDS             6660..7931
                     /locus_tag="VW852_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0272187.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            8042..9334
                     /locus_tag="VW852_001024"
     CDS             8042..9334
                     /locus_tag="VW852_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0272188.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            9336..10178
                     /locus_tag="VW852_001025"
     CDS             9336..10178
                     /locus_tag="VW852_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0272189.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            10431..11231
                     /locus_tag="VW852_001026"
     CDS             10431..11231
                     /locus_tag="VW852_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV0272190.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            11241..12227
                     /locus_tag="VW852_001027"
     CDS             11241..12227
                     /locus_tag="VW852_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0272191.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            12363..12443
                     /locus_tag="VW852_001028"
                     /pseudo
     CDS             12363..12443
                     /locus_tag="VW852_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(12743..13684)
                     /locus_tag="VW852_001029"
     CDS             complement(12743..13684)
                     /locus_tag="VW852_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0272192.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(13662..15425)
                     /gene="aspS"
                     /locus_tag="VW852_001030"
     CDS             complement(13662..15425)
                     /gene="aspS"
                     /locus_tag="VW852_001030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV0272193.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(15661..15741)
                     /locus_tag="VW852_001031"
                     /pseudo
     CDS             complement(15661..15741)
                     /locus_tag="VW852_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(15777..16007)
                     /locus_tag="VW852_001032"
     CDS             complement(15777..16007)
                     /locus_tag="VW852_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272194.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(16025..16666)
                     /locus_tag="VW852_001033"
     CDS             complement(16025..16666)
                     /locus_tag="VW852_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0272195.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(16678..17412)
                     /locus_tag="VW852_001034"
     CDS             complement(16678..17412)
                     /locus_tag="VW852_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV0272196.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(17415..17612)
                     /locus_tag="VW852_001035"
     CDS             complement(17415..17612)
                     /locus_tag="VW852_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272197.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(17612..17818)
                     /locus_tag="VW852_001036"
     CDS             complement(17612..17818)
                     /locus_tag="VW852_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272198.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(17828..18142)
                     /locus_tag="VW852_001037"
     CDS             complement(17828..18142)
                     /locus_tag="VW852_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272199.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(18413..18493)
                     /locus_tag="VW852_001038"
                     /pseudo
     CDS             complement(18413..18493)
                     /locus_tag="VW852_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(18484..19773)
                     /locus_tag="VW852_001039"
     CDS             complement(18484..19773)
                     /locus_tag="VW852_001039"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV0272200.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFATKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(19851..21098)
                     /locus_tag="VW852_001040"
     CDS             complement(19851..21098)
                     /locus_tag="VW852_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV0272201.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNDKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLAHTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(21289..22140)
                     /gene="rgg"
                     /locus_tag="VW852_001041"
     CDS             complement(21289..22140)
                     /gene="rgg"
                     /locus_tag="VW852_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV0272202.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKFDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            22264..22605
                     /locus_tag="VW852_001042"
     CDS             22264..22605
                     /locus_tag="VW852_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0272203.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            22663..22743
                     /locus_tag="VW852_001043"
                     /pseudo
     CDS             22663..22743
                     /locus_tag="VW852_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(22859..24562)
                     /gene="ilvD"
                     /locus_tag="VW852_001044"
     CDS             complement(22859..24562)
                     /gene="ilvD"
                     /locus_tag="VW852_001044"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV0272204.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(24795..25727)
                     /locus_tag="VW852_001045"
     CDS             complement(24795..25727)
                     /locus_tag="VW852_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV0272205.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(25724..26581)
                     /locus_tag="VW852_001046"
     CDS             complement(25724..26581)
                     /locus_tag="VW852_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0272206.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDENKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(26585..27931)
                     /locus_tag="VW852_001047"
     CDS             complement(26585..27931)
                     /locus_tag="VW852_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0272207.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(27944..28228)
                     /locus_tag="VW852_001048"
     CDS             complement(27944..28228)
                     /locus_tag="VW852_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0272208.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(28232..30262)
                     /locus_tag="VW852_001049"
     CDS             complement(28232..30262)
                     /locus_tag="VW852_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV0272209.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
BASE COUNT         9446 a         6452 c         5411 g         8995 t
ORIGIN      
        1 atctgcaacc tcaaagctgt actttgagca acctacggct agtttactct ttgattttca
       61 ttgagtataa actagacttc gcaaaagtag aaaacttacc gaaaatcttc aatatgacaa
      121 aaacgtataa gaacaggtac acaaacctta atcttatgcg ttttgttata gtttccatac
      181 gcattaaggg gaaatagttg aaatcagttc ttttaataac cattgaatac catatactac
      241 atttacccca aatatgatat aggccgtata aatcaagtat ttcgattttt cccgcctcaa
      301 agaatagatc ttatacaaaa taaataaatt taaaataata aataatagag acaaaatctt
      361 cctccaaaat caatcctaaa agtacacagt caagcatagc tctaatcttt attttcgatt
      421 cgaaattaca aacttatcaa tgaacaataa gtgatataag gtcagtacca aaccccaaaa
      481 gctaccacga aaagagaatt cttgatgatt gagagtaaac aaatcatggt taccaatata
      541 gaggcaggtc aaaataacaa ataaaatcca aaataaatcc gtccatttat attttgaaaa
      601 atattttttt aacataatag catatttttc ttgactataa taacagtata taataaaatg
      661 taagcagata caaataaaaa ggagaaaagc tcatgaacaa attaatgaaa tttatttcgg
      721 ttttttgacg tcaattgtgt taattgtatc agcgattcca agtgtttcag ctgtatacgc
      781 ttctgaacaa gtatcgcaaa ttgaaacaaa tatggaactt caacctgtca cttctctaac
      841 agaagaacaa atcaatacac ttgcaaacga aatccaatct tttcatccag acgtctcaca
      901 acaatggatc aaagaagtaa ttaaccgaca attacaaggc gattatacaa tcccacctac
      961 atactctcca tttagagcag cttggcaagg tattacagtt aatcaaatgg gtgctctatt
     1021 agatactgca atagctttag ccttaggagg aactactgca ggccttgcaa atctaattaa
     1081 agtaaaagga aaacatgcag caaaaagtgc tattcgttca gcaatttcta gatatctagg
     1141 tagttggttt gtaaatgatg ttgctttaga attcgctatg aatttattat caccggggac
     1201 ttatttagca caattatggg ataaaaatga tgccattcct aacaacggaa ggattaactt
     1261 ttaatcatga gatatacaat aaaattaatt gggtataacg tattttcgtt gtttatattc
     1321 tttatcttat caatgttgtt taaagttccc tttaggtggt ggatcagcat tgcaatgacg
     1381 acttcgtcgt acattgtata taattactta aagaaaaaga gagaaaagga atgatcttat
     1441 accagatcat tccttttctg ctttctgtaa atccctcgga gtgtgtttgg cgctttgata
     1501 cgaagtgtca aagctgtata aacgttacta tttccaaaaa agtaacagaa aattcctgct
     1561 ttgtaggact gctttgggtg agtaatagac atgtcgactc accctatctc ctgatggcga
     1621 aaagaattta gcaatgatgc aagttgttct gaactcgaga aaaaacatgc cgaaatcgaa
     1681 gaaaagcgtg cagaacttga agcgaagaag caagaaatgt tgaagcgctt ggataaatag
     1741 aatttagaaa acagaaaaaa ctaaaaagaa tgtagggttt atttccctac attcttaaaa
     1801 taacttagta cataaatgac aatgctgcca ctgccgtcaa tattgcaaaa actattttcc
     1861 aaattttttt attttttcca aaaatcttat caggtaacaa gcttattaca agaaaggtaa
     1921 taaacaacac caatttaagt gtgtatatca taaaaacacc ttctttccta caattatatt
     1981 ataatacttt atgtaagcga atacaagaga tatgaggtaa ccaactacga aaaaatataa
     2041 ggctgaagac agtaaaaacc agcatttaaa gccttcactg tatctaggat ttccctattt
     2101 ttagacaaga ttgacccaaa tccacagcat ttaaaaaagc gaatttcaat cgaaactcgc
     2161 ttttttacaa gatgtattag tataaatttg tatcttatac tcaatgaaaa tcaaagagca
     2221 aactaggaag ctagccgcag gttgctcaaa gcaccgcttt gaggttgcag ataaagctga
     2281 cgtggtttga agagattttc gaagagtatt agttcaagtg ccagatgtct tcgttatact
     2341 gagcgattgt acggtcagat gagaagaatc ctgctttaga aatgttaacg atgactttat
     2401 ccaaccatgc gtcacggtct tcgtagtcag caagcatttg ctctttgact ttgatgtagt
     2461 cttccaaatc aagaagagtc atgaaccagt ctttgttgat caattcattg taaagacgtt
     2521 ccaagcgctc tttgtttcca gctgcaagaa ctgcatcact aacgatgaag tcaaccaatg
     2581 gtttgatagc ttcacgagcg tagaattcgc ttgatttgta agctgctttt gcgtaaaggt
     2641 cgataacagt ttctgaatct tcaccgaaga tgtagatgtt ttcttctcca accaactcag
     2701 cgatttccac gttagcaccg tccatagtac caagtgtcaa agctccgttc aacatgaatt
     2761 tcatgttacc agtacctgaa gcttctttag aagcaagtga gatttgttct gagatatcac
     2821 atgctgggat aaggaaactt gctgcagtaa cgttgtagtt ttcaaccata actacttgca
     2881 agtgtggagc tactgctgga tcgttagcaa taacttctga catgcaaagg attaaatgga
     2941 taatgtcttg agcgattgtg taggctggag ctgctttacc accaaagaag attgtgattg
     3001 gacgagcagg gatgttacca gctttgatgt caaggtattt gtggatcacg tacaaagcgt
     3061 tcatttgttg gcgtttgtac tcatgaagac gtttgatttg gatatcaaag atagaatttg
     3121 aattgatttc cacaccttgg tgttctttca agtgacgagc caatttacgt ttgttgtgag
     3181 ccttgatgct ttccaatttt tctttgacag ctgctttgtc ttcataagac aaaagttttt
     3241 caagctcatc tgcttcatgg tgccaaccat ctccaagaat ctcatctaag tagtgagaca
     3301 atcttgggtt agcatgcata agccaacgac ggaaagtgat accgtttgtt ttgttgttga
     3361 acttttctgg gtaaaggtcg tagaaggctt tcaactcaga atttttcaag atttcagtat
     3421 ggagtgctgc aaccccgtta acactgtatc cgtagtggat atccatgtga gccatgtgaa
     3481 cacgtccgct ctcatcgatg atttgaacag ctggatcttt gtactctgcc ttcacacgac
     3541 ggtccaattc ttcgatgatt ggtaccaagt gaggaaccac ttcttgcaag aattcaagag
     3601 gccatttttc aagcgcttca gcaaggattg tgtggttagt gtaggcagtc atgctacgaa
     3661 caattgagat tgcttcgtca agctcgatac cacgtgcagt taaaagacga atcaattcag
     3721 gaatcaccat tgatgggtga gtatcgttga tttggacaac tgcgtagtca gcaaggtcat
     3781 gcaagttgct tcctttttcg attgcttcgt cgatgatcaa ttgcgcaccg tttgaaacca
     3841 tgaagtattg ttggaagata cggagcaatt caccttgacg gtcactatca tctgggtaaa
     3901 ggaagagagt caagttgcga gcgatatctg tcttgtcaaa gttgatacca tctttaataa
     3961 tagaagaatc aactgaatcc aagtcaaaca aacgcaagcg gtttttagtc gctgtttcat
     4021 aaccagtaac atcaatatcg taaagagttg atgtcaaagt aaagtctgca aatggtactt
     4081 ggtagctacg gcttgagcga accaaccagt tttgctctgt caaccatgca tttggaattg
     4141 tttcttgttg gttgttttta agaacttgtt ggaaaagacc aaagtggtag ttaagaccaa
     4201 caccgtcacc attcaaacca agagtagcaa ttgagtcgat aaagcaggca gccaaacgtc
     4261 ccaaaccacc attaccaaga gatggttcca attcaacttc ttcaacttcg atcaagtctt
     4321 tacctgcagc tgcaagttct tttttaacat cgtcgtaaag accaaggtta atcaagttgt
     4381 ttgacaagag tttaccaatc aagaactcag ctgagatgta gtaaactttt ttcttaccag
     4441 tgttgactgg tttttggctg cttgcaagct tgctgtagtt aagaagagca aggtaaagct
     4501 cttcattgct acattctgca atggttttat tgtaacgatt ttgtacaaat tcttgtagtg
     4561 ataacatgtt tagttgtctc ctgattattg tcttatttct ttaaatctac caaattttca
     4621 ttaattcggc gataaattgt tgtcaagtca agcaaacctt cctcgacagc tggtgtcaat
     4681 tgatcttcag tcatacgcca agaccagttt ccaccaaggg tagatgggaa gttcatacga
     4741 gctgcctcat ccaattctag taaatcttgc atagttgcaa ttgccataaa gctaactgat
     4801 gaaaatactg tacgaagcat agcgtgtacc actgtttcgt attctttacg gttcgtgtaa
     4861 cgagccatgt actcacgagt cgcatcatca atctcattac ggtaccaacc aagaaccgta
     4921 ttgttatcgt gtgttcctgt gtacataact gagttagcag gtgccaagtg tgggctatca
     4981 atgctttcgt cttctgggtt gaaggcaaat tgaagaatct tcattcctgg gaagccagta
     5041 cgttcacgca attcgatcac ttcatctgtc atgaagccaa ggtcttctgc gatgatgttt
     5101 agctcaccaa gttcttcctt aacggctgca aaaagcttgt agcctggacc tttcacccac
     5161 tcaccaggtg ctgctgtatc ggaaccagca gggatttccc agtaagattc gaagccacgg
     5221 aagtggtcga tacgaacgat atcgtagatt ttgaagcttt cacgcaagcg ttcaatccac
     5281 catttgtaac cgtctttgtc cattgcttcc cagtcataga ttgggttacc ccaaagctga
     5341 ccagttacag aaaactcatc tggtgggcat cctgcgatac aagtagcctt accattgaca
     5401 tctgttttga agagatgtgg atttgcccac atatcacttg aatcttccgc tacgtagatt
     5461 ggcatgtccc caacgatttc gatgtggttg tcgttagcgt aagctttcaa tttcaaccat
     5521 tgttggaaga agaagtattg agtcacacgg tggtaaacca acttgtctgc caattgctca
     5581 cgatagcttt caagtgctga agctttacga gcacgagcat ctgcatctgg ccattcagtc
     5641 caagcaagat tgtcaaaata ctctttgata gccatatact cagcaaagag ctcaagccat
     5701 gattggttgt cttgagcaaa tttctcaaaa tctttaacat ctccgacttc aaagaaacgt
     5761 ttcaccgctt tttctaaaag aggacgacgt gcatagtaga ttttagcata gtcaacttca
     5821 gacgcatcgc taccaaagtc aactccttca aggtcacttg cttccaacaa accttgctcc
     5881 accaagatat ctaaatcgat aaaatgagtg tttcctgcga aggctgagaa agattggtaa
     5941 ggagaatccc cgtaactagt tgctcctaat ggaaggattt gccagtaacg ttgttttgta
     6001 cggaccaaga aatcaacgaa gtcgtaagca ctttgaccaa atgatccgat tccgtaagct
     6061 cctggaagag aagagatgtg catcaacaca ccactttgac gttttttcat aatagcacct
     6121 cgtgtgttaa aataatggaa cgttgcgtat ttttcagacg caaacgtttg cgttacttat
     6181 aagtatactc cttttcaagc ataattgcaa gcgtttttaa aaaacttttt tgattatttt
     6241 taaggagact aactcatttt agcaaagata acttaaaact atctaatcct aaagtttgac
     6301 tctcacgaga cagtctacaa agtacaaaac ctccttaggg agcttgaatt tatatgcaat
     6361 aaagcacaaa cgtctataga aaatagatat agattagaaa cagggaggta gcccctcctg
     6421 atttccctct ttagacagat tccatatatt tttattaaaa atactgtatt cttatctatt
     6481 tcatactttt cttctattaa aatgcaaaat tatgctatca aatcaagctt aaaaattttt
     6541 taaaattttt acaaaaaata cttgcaaccg ttttctattt gtgctatact aagctcataa
     6601 aggaaaacgt ttgcgtttcc ttatcaataa aacttgctat tctttaggag gaatacacta
     6661 tgtcatctaa atttatgaag agcgctgcgg tgcttggaac tgctacactt gctagcttgc
     6721 tcttagtagc ttgcggaagc aaaactgctg ataagcctgc tgattctggt tcatctgaag
     6781 tcaaagaact cactgtatat gtagacgagg gatataagag ctatattgaa gaggttgcta
     6841 aagcttatga aaaagaagct ggagtaaaag tcactcttaa aactggtgat gctctaggag
     6901 gtcttgataa actttctctt gacaaccaat ctggtaatgt ccctgatgtt atgatggctc
     6961 catacgaccg tgtaggtagc cttggttctg acggacaact ttcagaagtg aaattgagcg
     7021 atggtgctaa aacagacgac acaactaaat ctcttgtaac agctgctaat ggtaaagttt
     7081 acggtgctcc tgccgttatc gagtcacttg ttatgtacta caacaaagac ttggtgaaag
     7141 atgctccaaa aacatttgct gacttggaaa accttgctaa agatagcaaa tacgcattcg
     7201 ctggtgaaga tggtaaaact actgccttcc tagctgactg gacaaacttc tactatacat
     7261 atggacttct tgccggtaac ggtgcttacg tctttggcca aaacggtaaa gacgctaaag
     7321 acatcggtct tgcaaacgac ggttctatcg taggtatcaa ctacgctaaa tcttggtacg
     7381 aaaaatggcc taaaggtatg caagatacag aaggtgctgg aaacttaatc caaactcaat
     7441 tccaagaagg taaaacagct gctatcatcg acggaccttg gaaagctcaa gcctttaaag
     7501 atgctaaagt aaactacgga gttgcaacta tcccaactct tccaaatgga aaagaatatg
     7561 ctgcattcgg tggtggtaaa gcttgggtca ttcctcaagc cgttaagaac cttgaagctt
     7621 ctcaaaaatt tgtagacttc cttgttgcaa ctgaacaaca aaaagtatta tatgataaga
     7681 ctaacgaaat cccagctaat actgaggctc gttcatacgc tgaaggtaaa aacgatgagt
     7741 tgacaacagc tgttatcaaa cagttcaaga acactcaacc actgccaaac atctctcaaa
     7801 tgtctgcagt ttgggatcca gcgaaaaata tgctctttga tgctgtaagt ggtcaaaaag
     7861 atgctaaaac agctgctaac gatgctgtaa cattgatcaa agaaacaatc aaacaaaaat
     7921 ttggtgaata aaaaatttgt tcaagggggg tggaaatcaa atcccccttt gaatttatca
     7981 atagagacac aaataattta gctttcttat aaaaaagtag tatcctatga aaggagttaa
     8041 tatggaaaag caacaaccta gtaaagcagc cctgctgtct atcattcctg ggttaggaca
     8101 gatttacaat aaacaaaaag ccaaaggttt tatcttcctt ggtgtaacca tcgtatttgt
     8161 cctttacttc ctagcacttg caacccctga attgagcaac ctcatcactc ttggtgacaa
     8221 accaggtcgt gataattccc tctttatgct gattcgtggt gccttccatc taatctttgt
     8281 aatcgtttat gtactctttt atttctcaaa tatcaaagat gcacatacga ttgcaaaacg
     8341 cattaacaat ggaattccag ttccacgcac actcaaagac atgatcaaag ggatttatga
     8401 aaatggcttc ccttacctct tgatcattcc atcttatgtt gccatgacct tcgcgattat
     8461 cttcccagtt atcgtaacct tgatgatcgc ctttaccaac tacgacttcc aacacttgcc
     8521 accaaacaag ttgttggact gggttggttt gaccaacttt acaaacattt ggagcttgag
     8581 taccttccgt tctgcctttg gttctgttct ttcttggact atcatttggg ctttggcagc
     8641 ttctacttta caaatcgtaa ttggtatctt cacagctatc attgccaacc aaccatttat
     8701 caaaggaaaa cgtatctttg gtgttatttt ccttcttcct tgggctgtcc cagccttcat
     8761 cactatcttg acattctcaa acatgtttaa cgatagtgtc ggtgctatca acactcaagt
     8821 attgccaatc ttggctaaat tccttccttt ccttgatgga gctcttattc cttggaaaac
     8881 agacccaact tggactaaga ttgccttgat tatgatgcaa ggttggctcg gattcccata
     8941 catctacgtt ttgactttgg gtatcttgca atctattcct aacgaccttt acgaagcagc
     9001 ttatattgac ggtgccaacg cttggcaaaa attccgcaac atcactttcc caatgatttt
     9061 ggctgttgcg gcacctactt tgattagcca atacaccttc aactttaaca acttctctat
     9121 catgtacctc ttcaatggtg gaggacctgg tagtgtcgga ggtggagctg gttcaaccga
     9181 tatcttgatc tcatggatct accgtttgac aacaggtaca tctcctcaat actcaatggc
     9241 ggcagctgtt accttgatta tctctatcat tgtcatctca atctctatga tcgcattcaa
     9301 gaaactacac gcatttgata tggaggacgt ctaagatgaa taactcaatt aaactcaaac
     9361 gtagactgac tcaaagcctt acttaccttt acctgattgg tctatcaatt gtaattatct
     9421 atccactgtt gattaccatt atgtcagcct ttaaagcagg taacgtctca gcctttaaac
     9481 tagatactaa tatcgacctc aattttgata actttaaagg cctcttcact gaaaccttgt
     9541 acggtacttg gtacctcaac actttgatta tcgccttaat taccatggct gttcaaacaa
     9601 gtatcatcgt acttgctggt tatgcttaca gccgttacaa cttcttggct cgtaaacaaa
     9661 gtttggtctt cttcttgatc atccaaatgg tgccaactat ggccgctttg acagccttct
     9721 tcgttatggc gcttatgttg aacgccctta accacaactg gttcctcatc ttcctctacg
     9781 ttggtggtgg tatcccgatg aatgcttggc tcatgaaagg ctacttcgat acagtgccaa
     9841 tgtctttaga cgaatctgca aaactagacg gtgcaggaca cttccgccgc ttctggcaaa
     9901 ttgttctacc acttgttcgc ccaatggttg ccgtacaagc tctctgggcc ttcatgggac
     9961 ctttcgggga ctacatcctc tctagtttct tgcttcgtga gaaagaatac tttactgttg
    10021 ccgtaggtct ccaaaccttc gttaacaatg cgaaaaactt gaagattgcc tacttctcag
    10081 caggtgctat cctcatcgcc cttccaatct gtattctctt cttcttccta caaaagaact
    10141 ttgtttcagg acttacaagt ggtggcgaca agggataatt tatccccgcc accctttttc
    10201 attttatact cttcgaaaat ctcttcaaac cacgtcagct ttatctccaa cctcaaagtt
    10261 gtgctttgag caacctgtgg ctagtttgca ctttgatttt cattgattat tagcaattgt
    10321 cactgtaaat aatatccttg tagcaagcaa tttttctcct agacttgaaa taaagcgcat
    10381 ttctctatat aataatactt atatagaaaa caccttttag aaagatacct atgcttccat
    10441 atccattttc ctttttttca agtattttgg ggtttcgtaa gcccctgtcc aaacgtttcg
    10501 ggctcaactg gtttcaactt ctctttacca gtatcttcct tatcagcttg tctatggtac
    10561 ccattgctat ccaaaacagc tcccaggaga cctatccgct agaaactttt atcgataatg
    10621 tctatgaacc tctgacaaat gaggttgtcc aggatctctc tgaacatgct acaattgtcg
    10681 atggcacatt aacttatact ggaacagtta gtcaagcctc ttctgttgtg attggtccaa
    10741 gtcaaatcaa ggaattacct aagaacttgc aactgcattt cgatacaaat gagctagtca
    10801 tcagcaagga aagcaaggaa ctgacccgca tctcttaccg agccattcag actgagagtt
    10861 tcaaaagcaa agacagcttg acccaagcaa tttctaaaga ctggtaccaa caaaatcgtg
    10921 tctatatcag cctcttccta gttctcggtg cgagcttcct ctttggtttg aatttcttta
    10981 tcgtctctct aggagctagt cttctccttt atatcactaa gaaatcacgc ctcttttcat
    11041 ttagaacctt taaagagtgc tatcacttta tcttgaactg tttaggattg ccgactctaa
    11101 ttacacttat tttgggatta tttggccaaa atatgacaac cttgattact gtacaaaata
    11161 ttctttttgt tctgtatctg gtcactatct tttataaaac acatttccgt gatccaaatt
    11221 accataaata ggagattttt atgcccgtta cgattaaaga cgtggccaag gctgctggtg
    11281 tttcgccttc aaccgtaacc cgtgttattc aaaataaatc aaccattagc gacgaaacaa
    11341 aaaaacgcgt tcgcaaggct atgaaggaac tcaactacca cccaaacctc aacgctcgta
    11401 gcttggtaag cagctatact caggttatcg gattagttct tcctgatgac tcagacgcct
    11461 tctaccagaa tcctttcttt ccatcggttc tacgtggcat ctctcaagtc gcatctgaaa
    11521 accactatgc cattcagata gcaacaggga aagatgagaa ggagcgtctc aacgctattt
    11581 cacaaatggt ctacggcaag cgtgtagatg ggctaatctt tctctatgcc caagaagaag
    11641 accctctcgt aaaactcgtc gcagaagaac agttcccctt ccttatctta ggtaaatctc
    11701 tatctccttt catcccactt gtcgacaacg acaatgttca agctggtttt gatgcgactg
    11761 aatatttcat caaaaaaggc tgcaaacgca ttgcctttat cggaggaagt aaaaagctct
    11821 tcgtgaccaa agaccgttta acaggctatg aacaggcgct taaacattac aaacttacca
    11881 ctgacaacaa tcgcatctac tttgccgacg agtttctgga agaaaagggc tataaattta
    11941 gcaagcgatt attcaagcac gatccacaaa ttgacgctat catcacaacc gatagcctcc
    12001 tagctgaagg tgtttgtaac tatattgcca aacaccagct ggatgtccct gttctcagct
    12061 ttgactcggt taatcccaag ctcaacttgg cagcctatgt cgatatcaat agtttagagc
    12121 ttggtcgtgt ttcccttgaa actattctcc agattattaa tgataataaa aacaataaac
    12181 aaatttgtta ccgtcaattg atcgcccaca aaattatcga aaaataagag actgggcaaa
    12241 gagtcgttaa aagcaaaaac gcatactatc aggtattgaa aaaacttgat actatgcgtt
    12301 ttattgtggg aagatttact tccttttcta ctgaaattga gtcttttccc aagatctttt
    12361 tatactcaat gaaaatcaaa gtgcaaacta ggaagctagc cgcaggttgc tcaaaacact
    12421 gttttgaggt tgtggataga actgacgaag tcagctcaaa acatggtttt gaggttgtag
    12481 atgaaactga cgaagtcagc tcaaaacatg gttttgaggt tgcagatgaa actgacgaag
    12541 tcagctcaaa acatggtttt gaggttgcag atagaactga cgaagtcagc tcaaaacatg
    12601 gttttgaggt tgcagataga actgacgaag tcagctcaaa acatggtttt gaggttgcag
    12661 atagaactga cgaagtcagt aacatatata cggtaaggcg acgctgacgt ggtttgaaga
    12721 gattttcgaa gactattaat cactaattat ctatctcaac aaatcttcct agaatatgaa
    12781 cattttccga gacagagaca aaggagcttg gatccacttg tgtcataatc tgtttaaatt
    12841 cattaaactc tgcacgtgta atgacagtga ttaaaactgc ctttctctcg tgattatagg
    12901 ttccttctgc atcgtggatc atggttgctc cgcggtgcaa ttttttatgg attttttcaa
    12961 ttaccttctc tggatgattt gtcacaatca tggcctgcat acgcttttgc ttagtaaaga
    13021 ctgcgtctgt cacacggcta gagacaaaga tggtaatcat agaataaaga gcgtatttcc
    13081 aaccaaaggt taaacctgct atcagcatga tagttccatt taccaagaaa gaaatactac
    13141 cgacattctt acccgttttc ttgcgaatag tcaggctgac gatatccgtc ccaccactgg
    13201 agatattgtt tcgaagagca aaaccaatcc ccaaacccat aacaacaccc ccaaaaaggg
    13261 aattgataat gggatcctcc gtcaaggttg ccacagggac aaactggata aagaaggaac
    13321 tcatagatac cgtgataaag gtaaagacgg tgaacttatg gccaatctga taccaagcta
    13381 agaccatcaa agggaagtta atggcgtaga agcttagcga aatcggaata tgaaaaccaa
    13441 accagtgatt actcaaggca gagataatct gtgccagacc tgttgcacca ctcgaataca
    13501 catgccctgg ttggaaaaag aaattaactg ctactgctga taaaaaacca tagaccagag
    13561 aggccgaaat cttctcatca tacttttctc gagagatact ttgtaagaca cgtaaaattt
    13621 ttatctgata agcaaagcgg cgcagataat agcgccaccg cttaattcgt tttgtttgtt
    13681 tcatcttctt ctacttgtaa gctgagttcc tctagttgtt tgagagcgac tgttgatgga
    13741 gcttgtgtca ttgggtcagt tgccttgttg ttcttaggaa aggcaatgac ttcacggata
    13801 ttttcttctc cagcaagcaa catgacaaaa cggtcaagcc cgatagccaa accaccgtgt
    13861 ggtgggaaac catagtccat ggcttcaaga aggaaaccaa actggtcatt agcttcttca
    13921 gctgagaaac caagagcctt gaacatgcgt tcttgaaggt ctttttggtt gatacgaagg
    13981 ctaccaccac caagctcata accgttcaag acgatatcgt aagcaatggc acgaacctta
    14041 gccaaatcac cttctaattc gtgagcagtc tcttcctgtg gaagtgtgaa tggatggtgg
    14101 gcgctcatgt agcggccttc ttcttcagac cattcaaaca tcggccagtc aaccacccaa
    14161 aggaagttga acttatcatt atcaatcaag ccaagctctt tagcaatacg tccacgaagg
    14221 gcacccagtg ttgcattagc cacttcaagc gtatccgcca caaagagaac caagtcctta
    14281 tcttcaagag caagcgctgt tgtcaattct tcttggatac cagtcaagaa cttggcaact
    14341 ggtccgttta attctccatc aaccaccttg acccaagcaa gacctttggc accatactgt
    14401 ttggctactt ccgtcatctt gtcgatgtct ttacgtgaat agttgtccgc agctcctttg
    14461 accacaatcg cttttacagc aagtgcttct gaaaagactt taaagtctac acctttgacc
    14521 acttctgtca agtcctgaag caacatgtca aaacgagtat ctggcttgtc agaaccgtaa
    14581 agagccatag catcatcgta tttcatacga gggaacggta gcgttacttc gatgcctttt
    14641 gtttccttca tcacgcgcgc gatcaagcct tctgtaatat cttggatttc ttgctcagta
    14701 aggaaggacg tttccaagtc gacctgagta aattcaggct ggcggtctcc acgcaagtcc
    14761 tcgtcacgga aacatttaac gatttggtag taacggtcaa aaccagcatt catcaagagc
    14821 tgtttcgtga tttgtggact ttgaggaaga gcgtaaaaat gccccttatt aacacgagac
    14881 ggcactaaat aatcacgcgc cccttcaggc gttgacttag aaaggaatgg tgtctccacg
    14941 tcgataaact ccaactcatc caagtagttg cggatagagt gggtcacctt ggcacgaagt
    15001 ttaagatttt ccaacatttc tggacgacga aggtcaaggt aacggtaacg caaacgtgta
    15061 tcgtcatttg cctcaatgcc atccttaatc tcaaatggtg ttgtcttagc tgtgttaagc
    15121 acaataagag ctgtcacgtt taactcaacc gcaccagttg gcaacttatc attggcttgc
    15181 tcacgcgcag cgacctgacc agtcacctca ataacaaatt cgctacgaag gctttcagct
    15241 gttgccataa cctctgcaga tactttttca gggttgataa ccaactgcat gattccttca
    15301 cggtcacgaa gatcgataaa gatcaaacca ccaaggtcac gacgacggcc aacccatcct
    15361 ttcaaggtta tttcttgtcc gatgtgttcc tcacgaacac gaccagcata catactacgt
    15421 ttcattattt ctctcctctt ttattctgtt actattttac cataaaagcg caggctcttc
    15481 atgaaaatca tcagaaaagt ttgccagtct ttaaaagtca ggtgaaagcc ctaaaaatta
    15541 gcgctaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac cgtaggtatg
    15601 gttactgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc tgacttcgtc
    15661 agtttcatcc acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg
    15721 ctctttgatt ttcattgagt ataatacaaa aatccgatga acttcaccgg actcttttat
    15781 tttgaatttt tgcctgcttt acgcttttca gcgatttcgg ctgcctttcg aggcaagaca
    15841 atttccgtta tgtaagccgt cccaaaacgc agtacacctg caataggagc aaagacaact
    15901 gctagatagt tatagaagaa atcgcctttg aaggcataag ctagcgctcc aatgatgaaa
    15961 aatagaacta ctgcctgaat cactgctaat aaaattactc gtttcatgtg acctcctgac
    16021 tctattatag catgagaatc atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa
    16081 tactgtagac cagacctttt ctgctaacgt aagccaaacc caaactaaaa ccaaggctaa
    16141 aatagacaaa aaattgttgc acatcacctg gaaaatgaat caaggcaaat agaagactag
    16201 ataccagaag aaaaatcagg gttcgtttac tattgtcctg cttaggaaag agatagcgtg
    16261 ctaacatccc tctaaaaaca atctcttccg tcaaaggagc aaaaataacc acagcaaaga
    16321 atgagaaaag tggttgagac aaggtcaagt ctgtcgctat ttgctgattt actgaaggat
    16381 catctggcaa gaagaattga acgaccagag ataagaacca aaccaaggca ggaagccaaa
    16441 taaatcggtt aaagccgctc ttctcaatat aaacaggagc tttctgatac catttgtaaa
    16501 tgccgtacac atatactcca gccaagacca catagagtag agtaacagca tagggtgaag
    16561 cgcctaaagc aagcgacgca gtcgcgagcc cctgaataaa gccatagata aagaaaaagg
    16621 atagaagggc tagaagaatc cagccaaggt ttttaagtaa tttcatagat aactccttta
    16681 tttgaaataa cgttttacca taggtaactg catcacattg atataaacat ggatggctcc
    16741 tacaagcaag aaagctagta actgaatctc tcctgtcaag aaagaaatga taataagaaa
    16801 aatatataag gctggtaaga catattggtg taattggaat aaaattcgaa aactctgttc
    16861 caaattagcc tgacgctccc cttcatcata agaatttata tagttcaaga catcctttgg
    16921 tgtagcgaaa aattccaaat caaactgacg aacaatcgca atggttttaa aaagagattt
    16981 ttgagcgact aagaatacca caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt
    17041 atgcaatata atcacctcac ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt
    17101 aatattaaaa gcaatggttc caaactcaag attccgatac atttgcacat aataggtttc
    17161 attcagatcg tcatccattt cctcttgata caaggaatga aattttctgc ttttctttaa
    17221 gaaattgaaa gtcaaaaaca tactaatgaa acctatcagt aaacaaatag ctgatatcca
    17281 tggcatcaag gcttttacat ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat
    17341 ccctacaaac atgcccaaga acccccccaa gacaatagac atcaaaaata acaatctacg
    17401 tttctttttc atattcattc tcctttttca cttgctagat ttttggattt cttttcaatc
    17461 cattcagtta ctgggatgag agcaaagtag acccaaacaa attggtcgct ttgataggga
    17521 ttaaaccagc ttaggtccat cccaatcagt agaaatacgc tgactaataa agctatgacc
    17581 actacataat aaatcacttt atacttgttc atcactcgtc ctcctccaaa cgaaataccg
    17641 attcgactgt ttcgttgaaa atttgagata ttttcagggc aatgataatg gatggggtgt
    17701 actcatcccg ttctagtagg ctaatggtct gtctggaaac ccctgccagt ttggctaggt
    17761 cggtttgatt gagaccatcg cgagctcgaa gctcttttag acgatttttt agttgcacgt
    17821 tacacaccta ctctccgtca aattcaacgg tttggatatc ttcaatacgt tgcaacttga
    17881 atttttcttt tcccgtatta tcaacactac gtagtttgac ccattcctca tcaacatcca
    17941 caacttccca gttatctggc ccaatataca ctcccgttat aattggttcc tttccaatca
    18001 tttcttgtaa taatctcgac atttctgcgt ttcctttctc ttttcgctca agttttttga
    18061 ttttattctc tagtttcttg atttttttag aattattaga ataaaagaaa atcataaata
    18121 gtataaatcc tagtacccac attataactc ctttctgctt cctatttctt aacttgaatt
    18181 cattgtaaca tatctttttc ttttttgaca attatagttt tcaaaaagtt tatgattttt
    18241 gtcattttgc aaaagaaaaa ggtcaggagt aagttcctga ccactttatc tatcattaat
    18301 actcttctaa aatctcttca aaccacgtca gcttcacctt gccgtaggta tggttactga
    18361 cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat
    18421 ccataacttc aaaacagtgt tttgagcaat ctgcggctag cttcctagtt tgctctttga
    18481 tttttattga gtataaaatc ctagtttttc aaagatttct gaaaagtttt ggctgattgt
    18541 ctcaagtgac acttgcactt cttctcgggt ttggttgttc ttgaccgtca cttgtccgct
    18601 ttcgacttcg ctctctccca gggtgatgag ggtcttagtc gcaaagacat cggctgactt
    18661 gaactgagct ttgagcttac ggttgaggta atcacgctct gctttgaaac cttgttggcg
    18721 aagggcttgg actagttcta gggctttgac atttgctcca tcacccaaga ctgcgatata
    18781 gacatctagg gcattttcga tagggagggc cacgccttgt ttttcaagga tgagaagcag
    18841 acgctctaca ccaagcccaa aaccaaatcc agcagtttca gggcctccaa agtaagcaac
    18901 caaaccatcg tagcgaccac ccgcacagac tgtcaagtca ttgccctcaa tctctgtgat
    18961 aaactcgaaa atggtgtggt tgtagtagtc cagaccacgc accatattgg tatcgatgat
    19021 gtaatctact ccaagatttt ccaacatctg acgcacagca tcaaaatgag cttggctttc
    19081 ttcatcaaga aagtccaaga tagacggcgc attctctact gccaccttgt cttctttttc
    19141 cttagagtcc aagacacgaa gaggattttc ctccaagcga cgttggctat ccttagacaa
    19201 ggtctccttg agcggtgtca aatagtcaat caaggcttgg cggtaggctg cacggctctc
    19261 aggatttcca agagtgttga ggtgcaattt gacaccttga ataccgattt ccttcaaaaa
    19321 atgggctgcc ataacgattg tttccacatc ggtagctgga ttgctagagc caaaacactc
    19381 aacaccaatc tggtggaatt ggcgcaagcg ccctgcctgt ggacgctcat aacggaacat
    19441 aggtcccatg tagtagaact tgcttggctt ttgcacttct ggggcgaaga gtttattttc
    19501 cacataggaa cggacaacgg gcgcagttcc ttctggacgg agggtaatat ggcggtcacc
    19561 cttgtcatag aaatcgtaca tttccttggt tacgatatcg gtcgtatctc cgacagagcg
    19621 gctgataacc tcgtaatgct caaaaatggg cgtgcgcact tctgcgtagt tgtagcgctt
    19681 gaaaatttca cgggcaaagc cctcaacgta ctgccatttg gcagattcag caggtaaaat
    19741 atcctgcgtt ccttttggtt tttgtaattt catagggaat cctctttaaa cttaatagtc
    19801 ttattttacc acaaataaag ggattaaaac agtgagaaaa aaattaggat ttagatatca
    19861 tttttgagat taagaattgt caaaaaaata gctagcaagg aaagaccaac aaatagcatc
    19921 caagtcaact gtatattcca tacggctact agtgaaaaac aagctgttcc cacaggtatg
    19981 gataaggtaa acaatagacc taaaaaatta ctagtatgag ctagaacctc tggagctaga
    20041 tttttcatga gcatggcact aatctttggt tgaactttac cagacacata cagagtaaag
    20101 aagagaaata gcaaaccaag cacgacttga ttgaataaat tagccaaacc aactagacta
    20161 agtcctacgg tcgcccacat catcaatcta ggcaaggact gcttcccaaa ataatcattg
    20221 cccgtaaggc tactgatgat gattgctact aaaacacaga attgattgat aaatagtgcc
    20281 tctgtataag aaaaattcaa gagagaatgg ctcaaaaaga agatattata aattccaccc
    20341 aaagcgccac ccaaggaatt aataagcaag acagcaaaga gcataaaacc aaagtttttc
    20401 tgtccacttt taagaaaaac gagacgtaaa tttcggtaaa ttgttaggaa ctggtctttg
    20461 atagaaagct tatcattttt taagttttca ccatcagcag atgacattga caggctcaat
    20521 ttgctttttc ctaaaaagag gatagtggct gatactagga aaaagcaggc attgattccc
    20581 gcaacgagag aaaaattgtt gaccgataga gctaagagcc agactccgaa agcttgacca
    20641 ccaatagctg aaatataggt gatgaactgt gaaaaagaat aagcctccat caaatcatct
    20701 tcagctactt tttccttaat aagaggcata cgcaggccac ctgcaaaatc actgatgata
    20761 tcactaatga cattgatcaa acacaggcta gaaaaggcaa agagactagc ttgctgaaca
    20821 actagggctg ctagaaaaaa tagaaccgcc tgaaacaaac cgctatagac catccatttg
    20881 accttgtccc tcgtgtaatc tgcccgaatc cctacaaaaa ctgtaaagag ggtcggaaga
    20941 atcatgacaa tattcgccat agcaacagca aaagatgctt gtgacaaggt cgatgcatag
    21001 acgataaaga ccaggttgaa aatcgaagca ccaaaagcat tgaagaagcg tgataaagtc
    21061 aaaattctat aagctggatt tctaaacaat agtttcatag ataatctccc tccttgttct
    21121 aagttctaca tcaagaatag ctttagtata gcacttatga atttgcgaaa cgattctttt
    21181 gaatatatgc aacaaacaaa atgagcaaaa aaacgataga acttttgtaa tgacctgaga
    21241 gagggcaaca gttctatcgc ttcaaatttt ttcttggttt acagatattc aacattcggt
    21301 cgtatttcta tagtattcgg cagatttatt acagccaagc atctcaaaaa tacggacagc
    21361 atcctccatc tttttctgac cttccttgac tctaccttgc ttgctatcaa ggagaccttc
    21421 tgcccacaga taaacaattc ggaaataggt ctcattttcc ttgtagaaat gctcttcgat
    21481 aacacgttta aaataatagg cattggtaaa ttcttcacac tcaatactag ctaaaaagcc
    21541 attcaatagt atagtatgaa aaaggtttcg attgccagac atttccatta gaaaatcgaa
    21601 tttacgtacc atttctcgta catatctagt aaaaagagaa acggataaaa atggagaact
    21661 gactgaaaat aaattgagtt catagattcc ccagatctcg gtagaaaaca aataatcatg
    21721 aaggactttt ccttcctctg ctgttaagtc taccctttca tctatgctct tcatataaga
    21781 cttgatcata atggcattta gaatatgttt ctgtttgttg tgagaatggg catgcttttg
    21841 atactccctg cgatataagt cctcaagagg tgctatattc tttggttcca agacatctgt
    21901 aatttctttt ctcaactcag aatctgtatc atactggaaa cctctcgcca gaaagaggat
    21961 ctcctccaca ctggcagata tattttccag agcaaataga aacttttcca ccgaaagctc
    22021 actctgacct gtttcaaaac gggacaacat agacggcgaa aattgtcctc cggttgcttg
    22081 tctcagtgag atatttcttg attctcgtaa ttgtctaaag acttttccaa tctgctccat
    22141 agacttcccc ttgattccgt attttcttca ttttatcata tttttcagaa aattcatcaa
    22201 aaacttgcca aattgtcaga attatgagaa aatagaggat atttatcacg tggagggact
    22261 gctatgagag acgatatcaa aatcaatgac cgtgctttgg ccttgcaaga ccaaattatc
    22321 gaaaaactag agaaagtttt tgatacagat gtggaattgg atgtttacaa tctaggtctg
    22381 atttatgaaa tcaatctgga tgaaacgggg ctctgcaaga ttgtcatgac cttcaccgat
    22441 actgcctgtg attgcgccga aagcctacct attgaaatcg tggcaggtct gaaacaaatc
    22501 gagggtatca aagatatcaa ggttgaagtt acctggtcgc ctgcttggaa aatcacacga
    22561 atcagtcgct atggccgtat tgcccttgga ttaccacctc gttaagcagg ccaatcactt
    22621 ttaaagatga aaaaagcaag ccgaaaatgg cttgcttttt gatactcaat gaaaatcaaa
    22681 gggcaaacta gaaaactagc cgcaggttgc tcaaaacact gttttgaggt tgtggataga
    22741 actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt
    22801 aaccatacct acggcaaggc gacgttgacg tgatttgaag agattttcga gtatgagttt
    22861 attttttacc tgacttgtcc atattccaga agtctgtcac ggctccgcgt gaagcagatg
    22921 atacgatgtg ggcatattta ccgaggacac cacggctgta aagtggtggc aaggttgttt
    22981 ctgccttgcg tttttcaagt tcttcttcgg atacagccat ggaaatttct ttggtatctt
    23041 ggtcaaccgt aacgatatcg ccggtacgga gataggcaat tggtccacca tcctgagctt
    23101 caggagcgat atgtccaaca accagaccat aagtaccacc agagaaacgt ccgtccgtca
    23161 agagggccac cttatctccc tgacctttac caacaatcat tgaagaaagt gatagcatct
    23221 caggcatacc aggaccacct ttaggtccaa caaaacgaac aacgactaca tcgccatcaa
    23281 cgatttcatc tgtcagaacg gcctgaatcg catcttcttc tgagtcaaag accttagctg
    23341 gtccaacgtg acgacgcact ttaacacctg ataccttggc aactgcaccg tcaggagcaa
    23401 ggttcccgtt caagatgata agcggaccat ccgcacgttt tggattttca agtggcatga
    23461 taactttttg gcctggagtc aagtctgcaa agtcagccaa gttttcagct acagtcttac
    23521 cagtacatgt gatgcgatct ccgtgaagga aaccatttgc caacaagtac ttcataaccg
    23581 cagggacacc accgacttcg tagaggtctt ggaagacata ctgaccagat ggtttcaagt
    23641 cggccaagtg aggcacacgt tcttgaatcg tattgaagtc ctcaagtgac aagtcaacat
    23701 ttgcggcatg ggcaatggcg agcaagtgaa gagtggcgtt tgtagaacca ccgagagcca
    23761 tcgttacagt gatagcatct tcaaaggctt cacgagtcaa gatatctgat ggtttgagac
    23821 caagttccaa catcttaaca acagcacgtc ctgctgcttc gatatcttct ttcttatcag
    23881 ctgattcagc tgggtgagag gatgaccctg gcaaactcat ccctagaact tcgatagcag
    23941 ttgccatggt attagcagta tacataccac cacagccacc agggccaggg caggcattac
    24001 attcaagacg tttcacgtcc tcagctgtca tgtcaccgtg gttccatttt ccgatacctt
    24061 caaagacaga aaccaagtcg atatctttac catcaagatt tcccggtgca atagttccac
    24121 cataggcgaa aatagctggg atatccatat tagcaatagc aatcatagat ccaggcatgt
    24181 tcttgtcaca gccaccgata gcgacgaagg catccacgtt gtgaccactc atagccgcct
    24241 cgatggagtc cgcgatgatg tcacgagatg ttagagagaa acgcatacca ggcgttccca
    24301 tagcgatccc gtccgctacg gtaatggttc caaactgtac aggccaagcg cctgcagatt
    24361 tgacaccttc tttagccagt ttcccgaaat catgcaagtg aatgttacat ggtgtatttt
    24421 ccgcccaagt cgaaatcact cccacaatcg atgtttcaaa gtccttatct gtcataccag
    24481 tcgcacgaag catagcacgg ttaggtgatt taaccatgct gtcatagatg ctactgcggt
    24541 gacgtttatc taattcagtc atcttatccc tcccatttca gtttttacta ttatagcaca
    24601 atttaaaggc aatgaacaga ataaaattct tgaattttca gaaaattcta tacgcatgtg
    24661 aaatatttaa aattaaaaac aacaaagcgg attagtgcac tttctgatga ccagaatatg
    24721 ctttttaatc cgctttcttt aaataacgta ctgtaatttt tacagaaatt ctttcagata
    24781 agtgtattta acatctatct tgcattataa atttctagaa ccttctcttt tatattcgat
    24841 tcactcaaac catactcatt aagaagataa tccattttcc ctacttgacc gaatctttct
    24901 tgaacaccca tccgatgaat ttttgttatt ccatcatcag agaataattc acataaagca
    24961 ctgccaattc cacctatctg attgtggttt tctacagtaa atatagtttt tccacttaac
    25021 attgttttta tctgttctgg tatcggtttg attctaaata aatctatcac acctactgaa
    25081 taacctaatt tagacagttc atctgcaact cgaatacttg gagcaaccat tatgccagaa
    25141 gcaacgatta caagatcttc accatgtctt aactcaatgt agcccttaga aaaatcttct
    25201 ccaccttgat atacagcctc tggagctttt ctaattgttc gaatatattt tagtcctttt
    25261 aagtctaatg tctgattcaa tatttcacga aattggatat catcagttgc ttcaaaaatg
    25321 attgatttag gaattaaacg taacaatcca atttcttcaa atggcatatg tgtcccacca
    25381 ttcatttctg cagttactcc tgcatctgat ccaatcacag tggcatccaa ttgtgcgtat
    25441 ccaagagaaa taaataattg atcaaatact cttcgtgaag caaaaggacc aaatgtatga
    25501 agataaggtc taaacccctg aatagacaag cctgctgcaa ggccgaccat ttctgcttcc
    25561 ataatcccaa cattcacata acggtctccg aagtcctttt caagattatg agtagccatc
    25621 gaacttgaca aatcggcttc taagactact atatcagaat aactttgatt agcctctaaa
    25681 aggaagtctc tatatacatg ccgtaattct ttcgtacttc tcatcattct gtttcctcca
    25741 attcctgact taatctttct acaactgaag ttaacatttg tttctcctct acagtagggc
    25801 gaagatgatg attggatttc atttcttcca gctcttgaac cccttgacct ttaatagtat
    25861 ctaatacaat gcacttaggt gatgaattat ttgactgttt taattggaca atcccttcat
    25921 aaatttctct aatatctgaa cccttgaccc taatggattc aaatccaaat gctgaaaatt
    25981 tttctacgaa atcacctgga ttacaaatat cctttgtaaa accatctaat tgttttttgt
    26041 tctcatcaac aaatacaatt aagttggata actgttgatg agaagcaaac tgtatagcct
    26101 cccaacattg tccttcattt aactcaccat ctccaacaat agcataagta taaaagggac
    26161 tctttcttat tctctgacca tatgcaagtc cagttgcaac actaattcct tgtcctaaag
    26221 agcccgttgt catatctatg cctggcgtta gatttctatc aggatgagac ggtaatttgg
    26281 ttccatttgt atttaaagaa tataagaatt ctttgtcaaa gaaaccattc aaatagagtg
    26341 tactgtatag agctggtcct ccatgacctt ttgataatat gaaataatct ctatctcgtg
    26401 ctgcaaatat ttctggagtc attggcatta tttcaccata aagcactgct aaaacttcta
    26461 cgatagacag acttcctccg taatgtccga atccaagatg attcaatgtt ctaagagtat
    26521 ttaatcggat gttagtcgca aattttctta actcatcttc tctattttta cttaaaatca
    26581 tcccttattc ctcctttgca gatggctttt taataaagga tactccaaac ataactgcta
    26641 aaataagaac aagaccaatc acaatgcctg cttgtgagcc aaattgattt aacattccta
    26701 aaataattcc tgatagacca aaatctgcat ctgagaaagt tgatccttgg aaaccgagtc
    26761 ctcccaaaac tggcattaaa aagactggga gaaaactgat taaaatacct tgtaaaaatg
    26821 ctccaatagt ggctccacga acaccaccag atgcattccc aataacacct gcagttgctc
    26881 cacagaagaa atgaggcaca acacctggta agataacaac cgttcctgaa gcaatcataa
    26941 ttgccatact tactaaacca ccaacaaaac tagagataaa tccaattaga actgcattgg
    27001 gtgcataagt ataaacaatc ggacaatcca aagcaggttt tgaattaggt acaagacgct
    27061 ctgaaatacc tttaaaggct ggaacaattt cgcccaaaat aaggcgaaca cctgctaaaa
    27121 taacaaatac ccctgctgca aattgacctg ctaattgtaa agcataaact agaccacttg
    27181 taccactact gatttctttt tctatatatt ctgaccctgc aaagatagct acaataatgt
    27241 aaataactgc catagataaa gtgatactaa cagtactatc acgtaaaaaa gctaaactct
    27301 ttggaaattt aatgtcctct gttgattttg atttgtcacc gataaggcta ccagtaaaac
    27361 cactcaacca atatcccaaa gaactgaaat gacctaaagc caccttgtca tttccagtta
    27421 attgaaccat atatttttgc acaaatgctg gggaaatact cataataata ccgagtgcta
    27481 atcctcctag taagataaga ggcaagctag taaagccagc aactgataaa atgaccgcaa
    27541 tcatacatgc catatataga gtgtggtgcc ctgttaaaaa aatatattta aatcgagtaa
    27601 aacgagcgat taagatattg aacaccatgc ctgcaaacat aatcattgca gtagctgagc
    27661 catatgttgt taaagctact gctacaattg cttcattatt cggcacaacg ccagataaat
    27721 gaaaagcatg ctcaaacatg gtaccaaatg gatttaaaga attttgtaca attcctgcac
    27781 caccagatac aactaagaaa ccaacaaagg tcttaattcc acctttaata atatcaggta
    27841 atttcttctt ctgaagaact aatcctaaaa ttgcaattaa agctactaaa atagctggtg
    27901 tactaacaat atccaatatg aacttcatca tgacgctagc ctcctatata agtccttttt
    27961 cttcacaaag tttagtaatt aattctcgta gttcatccat atcaataata ctatttaaga
    28021 tacgaacatc tccaagatga ctagctgaat cagctagatc acgaccaaca atccaaatat
    28081 cagctgcatt tggatctgct ccacctaaat cataatgttc aacttctaca tccgaaacat
    28141 tcaaatcact caatacagat tcaatattca tctgtaccat aaaacttgaa cctaatcctg
    28201 aaccacaagc tgtaccaatt tttaacatta tctaatcctc ctgtttaatt atcattttaa
    28261 tatcattata gttttttgat gatattaaag tttgaacatg atttttatct cttaaaattg
    28321 ttgttaaatg tgacaaagcc tttaaatgac tctcattatc aatggctgca atacaaatca
    28381 acaatcttac ctcttgttct ggattatcca ataaataaat cggttcttcc aaaactaaca
    28441 ttgacattcc tatttcattc acaccttcat ctggccgagc gtgaggaatt gctactccct
    28501 tccctaaatt aataaaaggt ccaaactctt ctactttttg aatcattgcc tcagggtagt
    28561 tctcagttat cttatcttga tccaaaagcg gtttagctgc taaacgaatc gcctccttcc
    28621 atcctaattt ttgcgaacta acctgatagg tttctttggt aataagttgt tctagcactg
    28681 gtacaatttc ctttctatca tttttttggt aaagataatt ctttaacgcc aatcttaatt
    28741 ccaattcttg tgtaataatt ccatatcttt tgacaatatt caggatttgt tcaatctcaa
    28801 aatctccata ctctaaattc ggaaaatctt ttaacactag ttctactagt tgtattgctt
    28861 gctcttcagt catcataacc gaaactagat aatttggctt ttctgtctcc acctttatgg
    28921 tagaaaaaac catatcatag tcactactag ctttcacctg taaatcatca atctttgagg
    28981 ttcctataaa ctcaatttga ggaaataatg ctaatagatt ctcttttaac atcaatgaag
    29041 aactaacacc attaggacaa atgattgctg ctttatagca tttttgaggc aaagtatctg
    29101 ctttctttaa ataacctccg aaatggataa caaaatatgc tgtttcacta tcaggtatgg
    29161 gattgtcaat agcgtccatc aagggcatca aagaatcttt gactaattca aataaatcag
    29221 gataatgttc tttaacatgc aatacatatt catttgaact aggtaggccg aactttaatc
    29281 tatagtaagc cggtataagg tggcggcgaa gattttctct caatccttcc ctttgtttaa
    29341 aatgtaacaa agaaatatct tccattctac ttataatagc ctctgttaat tgattaaagt
    29401 aaaccggagc aacatctact tcaccttcaa agcaacttga taataaaacg gtgatatagc
    29461 gataatcatc ctcagaaaac accgtatcta taattcccaa atcaaccact gtatccaata
    29521 aaatagtggt tatatcttga ataacaggag atactaatgt ctctgaaaga catactcttt
    29581 caacatccct ttgataccta cacagaatga atactaaacc gaaaaggtaa acttttaatt
    29641 gattaacaat aggtactagc tgtagcttct cataataatc tttaactacc tgatcaatca
    29701 aatcataagt taatgaatac ccccaactgg ataaaacata atccaaaccc caaatcccta
    29761 tggaggattc cagcaactca ctaaccattt gaaaagctaa gcggtgctta ttccactctg
    29821 aaccgtgtaa agtataacct tttgctctac tgtaccctag ctccaaatca ttatctaaca
    29881 taatctttct taatgattga atatcagata aggttgtatt cttacttact ttcaaaaagt
    29941 cttggtaatg attattcgat ataaaatcta atcggcaaaa agtgtaaaga tagattaaag
    30001 ctaagcgagt cgactttggt aaaaccaatt catccgactt aataatatct gtcaaagact
    30061 gcttcgtacg atttgataaa ctatagcgac cttgcttttt atccagcact atccctttat
    30121 tagctagata aggcactaaa taatctattc cttctttgac ttcctttata ggtaagctca
    30181 ccttaacaga taattcatat aatgatagct cacaatgatc catcaaagtc atcaaaataa
    30241 ctagtgctct ataatcaaac atttctcttc ctttctaact acaattatag tgtaaaaatt
    30301 ataa
//
LOCUS       DAVKWS010000022        29108 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKWS010000022 DAVKWS010000000
VERSION     DAVKWS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.022"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..595
                     /locus_tag="VW852_001050"
     CDS             <1..595
                     /locus_tag="VW852_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV0272210.1"
                     /translation="KYILSDLPFVSIDRHFSENVCYVTSANYKGGQTAAEELVRRDNK
                     HLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKEPLINPEDQIRIFLQSHKE
                     IDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKTSVDQSYLVSTIVQDIGMM
                     AKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            723..2033
                     /locus_tag="VW852_001051"
     CDS             723..2033
                     /locus_tag="VW852_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV0272211.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            2129..3031
                     /locus_tag="VW852_001052"
     CDS             2129..3031
                     /locus_tag="VW852_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0272212.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            3033..3878
                     /locus_tag="VW852_001053"
     CDS             3033..3878
                     /locus_tag="VW852_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0272213.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            3888..5207
                     /locus_tag="VW852_001054"
     CDS             3888..5207
                     /locus_tag="VW852_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0272214.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVATKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(5419..5491)
                     /locus_tag="VW852_001055"
     tRNA            complement(5419..5491)
                     /locus_tag="VW852_001055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5456..5458),aa:Lys,seq:ctt)
     gene            complement(5756..7027)
                     /locus_tag="VW852_001056"
     CDS             complement(5756..7027)
                     /locus_tag="VW852_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV0272215.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            7297..7497
                     /locus_tag="VW852_001057"
     CDS             7297..7497
                     /locus_tag="VW852_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272216.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            7701..7880
                     /locus_tag="VW852_001058"
     CDS             7701..7880
                     /locus_tag="VW852_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272217.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            8017..8196
                     /locus_tag="VW852_001059"
     CDS             8017..8196
                     /locus_tag="VW852_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV0272218.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            8233..8685
                     /locus_tag="VW852_001060"
     CDS             8233..8685
                     /locus_tag="VW852_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV0272219.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8976..9446
                     /locus_tag="VW852_001061"
     CDS             8976..9446
                     /locus_tag="VW852_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV0272220.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     AIFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            9465..10553
                     /locus_tag="VW852_001062"
     CDS             9465..10553
                     /locus_tag="VW852_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV0272221.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            10534..10962
                     /locus_tag="VW852_001063"
     CDS             10534..10962
                     /locus_tag="VW852_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0272222.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            11097..12050
                     /locus_tag="VW852_001064"
     CDS             11097..12050
                     /locus_tag="VW852_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV0272223.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNT
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRESAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            12052..12795
                     /locus_tag="VW852_001065"
     CDS             12052..12795
                     /locus_tag="VW852_001065"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV0272224.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            12806..14602
                     /gene="pepF"
                     /locus_tag="VW852_001066"
     CDS             12806..14602
                     /gene="pepF"
                     /locus_tag="VW852_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0272225.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEATFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            14848..15639
                     /locus_tag="VW852_001067"
     CDS             14848..15639
                     /locus_tag="VW852_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV0272226.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEVVLADVGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQELNAGFRNDLSNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPINRVHEVFNILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            15785..16417
                     /locus_tag="VW852_001068"
     CDS             15785..16417
                     /locus_tag="VW852_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV0272227.1"
                     /translation="MLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAIVVAAYSIGTVSG
                     AHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSNSGMSTASLGENA
                     LANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLSLMAMILVGLKIT
                     GLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFLGTEE"
     gene            complement(16442..16699)
                     /locus_tag="VW852_001069"
     CDS             complement(16442..16699)
                     /locus_tag="VW852_001069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272228.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSSKHCFEVADRTDEVSSK
                     HCFEVADRTDEVSNHTYGKVKLTWFEDIFEEYHTKKPCSSR"
     gene            complement(16761..17294)
                     /gene="queF"
                     /locus_tag="VW852_001070"
     CDS             complement(16761..17294)
                     /gene="queF"
                     /locus_tag="VW852_001070"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV0272229.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(17599..17913)
                     /gene="trxA"
                     /locus_tag="VW852_001071"
     CDS             complement(17599..17913)
                     /gene="trxA"
                     /locus_tag="VW852_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0272230.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(17930..18151)
                     /locus_tag="VW852_001072"
     CDS             complement(17930..18151)
                     /locus_tag="VW852_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0272231.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(18250..18630)
                     /locus_tag="VW852_001073"
     CDS             complement(18250..18630)
                     /locus_tag="VW852_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0272232.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(18775..19728)
                     /locus_tag="VW852_001074"
     CDS             complement(18775..19728)
                     /locus_tag="VW852_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0272233.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RRTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(19796..21004)
                     /gene="sstT"
                     /locus_tag="VW852_001075"
                     /pseudo
     CDS             complement(19796..21004)
                     /gene="sstT"
                     /locus_tag="VW852_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21143..21703
                     /locus_tag="VW852_001076"
     CDS             21143..21703
                     /locus_tag="VW852_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV0272234.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            21760..22668
                     /locus_tag="VW852_001077"
     CDS             21760..22668
                     /locus_tag="VW852_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0272235.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            22620..23210
                     /locus_tag="VW852_001078"
     CDS             22620..23210
                     /locus_tag="VW852_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV0272236.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            23213..24319
                     /gene="yqeH"
                     /locus_tag="VW852_001079"
     CDS             23213..24319
                     /gene="yqeH"
                     /locus_tag="VW852_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV0272237.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            24533..24844
                     /gene="yhbY"
                     /locus_tag="VW852_001080"
     CDS             24533..24844
                     /gene="yhbY"
                     /locus_tag="VW852_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV0272238.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            24896..25525
                     /locus_tag="VW852_001081"
     CDS             24896..25525
                     /locus_tag="VW852_001081"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV0272239.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            25525..26118
                     /gene="yqeK"
                     /locus_tag="VW852_001082"
     CDS             25525..26118
                     /gene="yqeK"
                     /locus_tag="VW852_001082"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV0272240.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVGKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            26119..26619
                     /locus_tag="VW852_001083"
     CDS             26119..26619
                     /locus_tag="VW852_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0272241.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            26632..26985
                     /gene="rsfS"
                     /locus_tag="VW852_001084"
     CDS             26632..26985
                     /gene="rsfS"
                     /locus_tag="VW852_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV0272242.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            27088..27834
                     /locus_tag="VW852_001085"
     CDS             27088..27834
                     /locus_tag="VW852_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0272243.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFSMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            27844..28941
                     /locus_tag="VW852_001086"
     CDS             27844..28941
                     /locus_tag="VW852_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV0272244.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARESDLPEGIHVLGFTEKGRQHLKYLKGQVSLVSRIGKEPWDV
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
BASE COUNT         8722 a         5316 c         6569 g         8501 t
ORIGIN      
        1 caaatatatt ttatctgatt taccgtttgt aagtatagat agacattttt cggaaaatgt
       61 atgctatgtt acttcagcaa attacaaagg aggacaaact gcagcagagg aactggttag
      121 aagggataat aagcaccttg cttttattgg ttcaattaat atgtatgaga atgagactat
      181 actcagaagt aaaggattca gagaaaaagc taaggaatta ggagtagatg tctataattt
      241 tgaaatgaaa gaacctttaa taaatcctga agatcagata agaatttttt tgcaatctca
      301 taaagaaatt gatggtatat ttacaataaa tgatatgatg gctctgagag ttattagtgt
      361 tatgaaagaa ctggggaaaa tagcacctga tgattaccag ataattgggt ttgatggttt
      421 aaaaacttct gtcgatcaat cttatctagt ctctacaata gttcaagata ttggtatgat
      481 ggcaaaagaa tctgttgcaa tgatactgaa aatgattaag cgagaagaga ttctagaaaa
      541 acgaatcgta attcccacat atttttgggg agataaaaca acaaaaaaaa tttaaaaagc
      601 gcttgacaat taatgaaagc gctgttataa tataatcaac aaatgaaacg tttcaaaaat
      661 aggctagaag cttgtttaaa aaataaataa aaaatgaaac gtttcaaaaa aggaggaaaa
      721 tgatgcgtaa aggtacaatg ttatctgttg ttgcggggct atcgttagct atacttgcag
      781 gttgtagtgg aggaactaac tcaaaacaag cgagttcttc aaatgatatt aaagtctggg
      841 tacaattctc tgacgaaaca aatgaaggaa aagcttggca aaaagttgtt gataacttta
      901 atcaatcagg aaaaagtaag tacaaggttg taactgagta tattcctcgt agtggtagtg
      961 gtggaggata tgaagacaag gttaatgctg ctattacgac taatagtctt ccagatgtta
     1021 ttacccttga tggaccaaat acggcagctt atgcaaaatc taaagtaatt actccgctag
     1081 atgattatct aaaagataat aatatggatg atgttttgga cagtattaaa caacagggaa
     1141 cttataatgg gaaattctat gcatttggat tttctgaatc gaatgttggt gtttattaca
     1201 ataaaaaaat gttcaaagag gccggaattg cggaatcaga gcttccaacg ctagaaaagc
     1261 catggacatg ggacgaattt aatacgattg ctaaaaaatt gaaagatcat tataacaaac
     1321 cagcaattga ttttcgtatt aattcaaacg atgaaatgtt gccatatgct tatatgccgt
     1381 tgatttggtc aaataatggt tctgtcgtaa acgaagatgg aacaaaggca gaaggatact
     1441 tcaactccaa agagagtgta gaagcagttc aattcattca aaatcttgta aaagaaggct
     1501 atacaactgt tagtccagtc gaaaaaggtt ttgaaacagg agagtatcca atgcttttga
     1561 gtggttcttg gacgattgct gacatgaata caaactacaa agatattgat tttggtatcc
     1621 ttccttaccc agtatctagt aagacgaaaa aattagtatc cccttcaggt agttggcaat
     1681 tagctgtgac gacaaaatct gataaaaaag aggccgcatc tgaatttgtt aaatttgcta
     1741 caaatacaga atcaagtgaa attattagct tggggaattc ggtccttcca attcgtaaat
     1801 ctacaattga aaatatcaaa gataaagtat ccgaaccaat gcgtttcttg atggaacaaa
     1861 attcaaaaac agctcatgct cgcccagttg ttgtagcata tcctcaggtt tctcgagctt
     1921 ttcaacaagc aatgcaagat attagctatt atgaagaaaa tccaaatgtg caaaaagtct
     1981 tggatactcg tacaaaagaa atgcaaactg ctattgatca gtcactcaaa taatctgtaa
     2041 aaaagaaggt ggaaggaaaa tgtttggaac taccttcccc tttcttgtta atagcctagt
     2101 ttgatatata tgattaaagg agatggatat gagtaaagaa actgttgtgg atacaaaaag
     2161 acgtgaaaaa ataaaggata acatattagg ttatagtttt ttagctccag ctttaatttt
     2221 attaggtatc tttttagtaa ttccagttgg aatggtcatt tattacgctt ttacagatta
     2281 ttatttgttg acaccagatg aaagaaaatt tgttggactt gaaaatttta ttcgattaac
     2341 aaaagatccg attttcttaa agagcttctt gaatactttg aaatttgttg tttggattat
     2401 tccagttcaa ttgggagcgg ccttaggaat ggctttgatt gtcaataaaa agcgaaaagg
     2461 aaatatgttt tttaaagtgg ctttttttgc tccagttgtc atgtccttgg tagttatttc
     2521 tattctttgg ctttatcttt taaatccaaa tagtggctta ttgaatgcta ttttaaataa
     2581 ggtaggtatt gcttctcaac ctttcttgac tagtcctaaa caagctatgt atgcaattgt
     2641 ttttgtttct gcttggcagg gtgcaggtta tcagatgctt ttatttttag gaggaatgca
     2701 gaatattcca caagatgtct atgaagctgc tgaattagat ggattctcaa aatgggctca
     2761 gtttcgttat atcacgatgc ccctactaaa accaacagcc ctgtttgtat tgttaacaac
     2821 tttaatttct gcttttaagc tgattgtaca accaatggtt atgacgcaag gtgggccatt
     2881 aaactcaaca attactatgg tgtactatat ttatcaacaa ggttttactg atcgtcttgt
     2941 aggatattct agctcaatcg ctttagtatt tacaacgtta attggtatga ttagcttagt
     3001 acaacgtcgc gttctgaagg aggatgacta agatgaaacg atttaaacca acaacaatct
     3061 tagaatatat tctgttaact ttattggcag gactcttcct ttttcctatg gtttggatgg
     3121 tagtttcagc tatgaagcca gaagcggatg tttataaaaa tttgactagt tggcaagcct
     3181 tcttaccaag tttaaatccc gctgattggt ttaaacctta tcaggaagta tttgagcgct
     3241 ttagtatttt tacatatcta gggaacagca ttttttatgc tggaacgttt gctattggtt
     3301 ctattattgt caatgcactg gccggtttcg cttttgcaaa agtcaatttt tcaggtaaga
     3361 aaattttatt tggtttctta cttgctttac ttattattcc agtggaaact gtgttaattc
     3421 cacaatttac cattatcaat tccttaggtt tggtcaataa tcgtcttgca gttattattc
     3481 caggattggc gagtgtattt aatatttact tatttaggaa tttctttatt gcaattccag
     3541 aagagattct tgagtctgca aagatggacg gagcaagtat cgttagaatt ttctttagaa
     3601 ttatgcttcc aatgtctaaa cctgcagttg ctacagttgg tgttttatca tttatttcaa
     3661 gctggaatga ttatatctgg ccattgatgg ttttgacaga ttcttctaaa ttttcaatgc
     3721 aggttgcaat tacaactatt aatacaactc aaccagttta tatcaatcag gtaatggctg
     3781 tattaactat ttcaacgatt ccgttgattt tggtctatgt agtcgctcag aaatatattg
     3841 ttcaaggtct gggtggttcc ggaactggaa taaaatagga tatacttatg ataaataaaa
     3901 catttacagt agaaaaagcc aatcgtttta tagcagaaaa taaacatctc gttaatactc
     3961 aatataagcc tgaagaacat ttttcagctg agattggttg gatcaatgat ccaaatggat
     4021 ttgtctattt tcgtggagaa taccatctct tttatcaatt ctatccatat gatagtgttt
     4081 gggggcctat gcactgggga catgctaaaa gtaaggactt ggtgacttgg gagcacttgc
     4141 cagtggcact tgctcctgac caagattatg accgaaatgg ttgtttctca ggctctgcca
     4201 ttgtcaagga tgatcgcctc tggctcatgt acactggaca tatcgaagaa gaaaccggtg
     4261 tccgccaagt gcaaaatatg gcattttcag atgacgggat tcactttgaa aagatttccc
     4321 aaaatccagt tgcaactgga gcagacttgc cagatgagtt gattgctgct gatttccgtg
     4381 atccaaaact ctttgaaaaa gatggacgct attactccgt agtagctacc aaacacaagg
     4441 ataatgtggg ctgtatcgtt ctactagggt ccgataacct agtagaatgg cagttcgaat
     4501 ccatcttttt aaaaggggta gaacaccaag gctttatgtg ggaatgccca gattacttcg
     4561 agttagatgg gaaagattgc cttattatgt cacccatgcg ttatcagcgt gagggagact
     4621 catatcataa catcaactca tcgcttttgt tcacgggtaa ggtagagtgg ggagaaaaat
     4681 gttttatccc agaatcagtt caagaaattg atcatggcca agacttctat gcgcctcaaa
     4741 cattgttgga cgatcaaaat cgtcgtatcc tgattgcttg gatgcagaca tgggggcgta
     4801 cccttccaac ccatgaccaa gaacacaaat gggcatgtgc catgacttta cctagaattc
     4861 taagattgga agatggcaaa ctaagacaat tccctgttaa aaaaggccaa tatcaaatcc
     4921 aaatagataa agattgtcat taccacttag gaaatgatat agattatctt gaatttggtt
     4981 atgacagtaa tgcgcagcaa gtttacattg atcgtagcca tcttattcaa aaaattctag
     5041 gtgaagaaga acaggacact agtcgacggt atgtagatat tgaagctaaa gaattggaag
     5101 ttgttctaga taaaaattcc atcgagattt ttgtcaatca aggtgaagca agcttgactg
     5161 caacttatta cttaacggtg ccagctgagc tatcacgaat tgattaaaaa ttaagttatt
     5221 tctcctaaag aaaaagttct ctttctaaaa tagtggaaag aggacttttt gtgttttggg
     5281 tatataagct tagtttatgg tatttgtaaa attggtgttg gattatgatt taagctagtt
     5341 ttctaaagaa tttgaaaaaa attttattta agcaaaaaaa aaaaccttgg ttccaagact
     5401 tttcctgttg tatttagatg ccccctacag ggatcgaacc tgtgacccac ggattaagag
     5461 tccgctgctc tgccagctga gctaaggagg caaagaaaaa agctgtattg gtgccgaaac
     5521 ttcacggttt gtattgaacc cgcgcaatta agcaggtggg caactcgctc taactgaagc
     5581 tgtttccgtg tgagacggcc tacatgctgt tagaagactt ttgtttccct aataatacaa
     5641 aaaatagtcg gtcaacactt aagtgtgaag tcgtacacca cagcgtttct atatttatat
     5701 gataccactt ttttaaaaaa aatcaagagg aaagtgcaat tttttgaaaa ggatttcaga
     5761 tttttcgcaa atggtcgata gcttcctttc tttgagccaa agcccgttca tatttaccag
     5821 tatcttctgc ttggaaatag tgatgattac gaattttttc tggcagatag tcttgcttta
     5881 cccaatttcc aggatagttg tgtggataga gatagtcttg ggcattcccc agttccttgc
     5941 ttccactgta gtgcccatca cgtaggtgtc gcggaatagg caagtgccct gatgttttga
     6001 ggtcagcaag tgccttatcc atagctacat aggctgagtt ggattttgga gaaagggcca
     6061 aatcaatcac gacattggca atgagaatgc gggcttcggg gaaaccaatc ctttgtgcag
     6121 catccagagc agtcacggta tgaatctggg cctcaggatt ggctaagccg atatcttcat
     6181 aggcgataac agtcaagcga cgagcgagac taggcagatc cccagcctca atcaagcggg
     6241 cagtatagtg gagactggca tccacatctg agccacgaat agacttttgc agggcagata
     6301 gaacatcata gtgaccgtct ccatccttgt ccatagtgat gtagcttctc tgaagactat
     6361 tttccatgat gtctaaggtg atgtggcgaa tgccctcgtc attctcaggg gtagatagaa
     6421 cagccaagtc cagtgagtta aaggcagagc gaaggtctcc gtttgtagag gttgcgatga
     6481 aatccagcgc atcctcatct agttctattg gaaaatcaaa accacgttca gggttactta
     6541 gagctatctg cagggcctct ttgacgtctt ggttagacag aggttccaac tcgaaaattt
     6601 gaacacggct acgaatggca ggagtgacag agaagaaagg attttcagtc gttgctccaa
     6661 tcatgatgac cagtccactt tccaagagag gcaagaggaa gtcttgcttg gtcttatcta
     6721 gtcgatgaat ttcgtctagc aataggacga gaccaccaga aaatttagct tcttccgaga
     6781 tttcttgcag tcgcttttta ctatcaactg tcgcattgaa agttcgaaag gcatacttgg
     6841 tcgttccagc gatggcagag gcaatactgg ttttgccgat tcccggaggg ccatatagaa
     6901 tcatggagga caggcggttg gcttccacca tgcggcggat gatttttcca ggtccgacca
     6961 gatgctcctg accgatgacc tggtcgatgg ttttagggcg catgcgaagc gcgagattgt
     7021 ctggcatagc agtcctttct aacgtggatt tgagtagaat gactcatcta ctatatctta
     7081 ctatgtccat tctagcctat tagttgtgat atttcaatgg ttttgtaata atcagggagt
     7141 gagatagaat cttttttact atttaattta atgaggagag gtatcagcat gagagatttg
     7201 attttcctta aagctcaaaa taggatagtt gagcctaaag aagtggaagt gctgacttga
     7261 gaaataatat tgctaaatgt cattgtatga actataatga aaagagaggt gataagcaat
     7321 ggaaatgatg gaccaagtca ggaaattttg atttttacaa aacaaattcg tcattggatt
     7381 cttagtgatc aagttatttc agggaaaaga aaacttttct tccgggaaga tactccaaaa
     7441 gagattttag atttgtacga aaacattaaa tctaaacttg attttgctta ccaataagtt
     7501 tattctaata atgggttgaa aaaatatgaa aaatagaata attgatgttt ttgaagtagt
     7561 aaatcgcatc cttgtaataa ctgttgaaaa tcctgatttc gaagacttga gagttaatca
     7621 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg
     7681 taaggcgacg ttgacgtggt ttgaatttga ttttcgaaga gtatcagttt gtaaaaattg
     7741 gagataaaaa atatagagta cgcagtgttc ctatgattca ttcaactccg cctcaatctg
     7801 tcttagatcg agatactttt acaattgact atacagatga tgaattgctc gataaagaag
     7861 cagtttttac tactcattag aaaaataggt ctgaataaat tggcttccct tagaaccaca
     7921 tctaggggct tttatgtata atagaataat aagtatcact cctttacaat acgtgtaaca
     7981 cgtgttataa tggtgttgtc aggaggtaaa agcgctatgc ctatgacaca aaaagagatg
     8041 gttaaactcc ttacggccca tggttggata aagactagag gcggtaaagg ctcccatatt
     8101 aaaatggaga agcaagggga aagacttatc acaatccctc atggtgagct gaataagtac
     8161 actgaaagag ggataagaaa gcaagctggg ttgtaaaatt cagcccctgt tttttgatat
     8221 ggaggtagtg aaatgttagt tacgtatcca gctttatttt attatgacga cacagacgga
     8281 acagaagcga cttattttgt ccatttccca gattttgaat actcagctac acaaggagag
     8341 gggatttctg aggctttggc tatggggtcg gagtggctag ggataactgt tgcagatttg
     8401 atagagagtg atggagagtt acctcagcca tcagatatca atagcttgtc tttaattgat
     8461 aatgatcctt ttaaagatga tgaagatttc gtgtcaacct atgaccttga taaatctttt
     8521 atttctatgg tatctgttga tgtatcagaa tacctaggaa gtcaggaacc cattaaaaag
     8581 actttgacca taccaaaatg ggcagataag ttgggacgag aaatgggact taacttttct
     8641 cagactttga cagacgctat tgcagataag aaagttcaag cctaagatta agcgtggtat
     8701 cgtggtaata gtcaataaaa aagcacgtcc acttgtgcta gttactcgcc tattgatttt
     8761 atagatttat tagccctttc gagggctttt tatattgatt tttatgagaa atgaagaaat
     8821 cagactttta agaaaatcac tgatatcaag ggtttaaatg aggtaatatg gtataattag
     8881 gacataaaat aattttgtgg taagatggta gtatctattt tagcatattt ccgagcaatc
     8941 ggggcgatta aagagtcgca tagaaagagg acaaaatggc aacatacgga tttttagatg
     9001 ttttagagga agagttggac aagaactttc cctttgactt tgagattagc tgggacaaac
     9061 gcaaccatgc ggtagaagtg agttttctat tggaagcgca aaatgctgca ggtgtggaga
     9121 tggtggatga agacggagag gtttcatcag atgacattct ttttgaggaa gcggtccttt
     9181 tctacaatcc tgccaagtca accgtcaatg cagaagacta tttaacggtt atcccttatc
     9241 tacctaaaaa aggattttct cgcgagtttt tagcttattt tgccattttc ctcaaagata
     9301 ctgctgaggt tgggctagat accctcatgg actttttgga agatccagaa gcagaggaat
     9361 ttgtcatgga atggaaccaa gaagtctttg aagaaggaaa agttggtttg gaagagggag
     9421 aattttatcc ttatccaaga tactaggagt tggttggagg tgttatgaag aaaattggga
     9481 cttatttggt ttatgtgcta gcttttgtct ttattatgct agcttttgct tgtggaacaa
     9541 tcgcatttgc agagttgggg tattccgcag ttttagcctt tacttttggt tatgccttcg
     9601 ctcttctaag catgtattta atctttattc ttcatgagct gggtcatgct ttttgtggct
     9661 acttgacagg ctatcggctg gtggcttttg gattaggaca ttttattttg accaaaaagt
     9721 caggcaggtt tcatcttagt agaacagcca ttctgaaaaa tgttggtgct caatacattg
     9781 gtttaaaaga ggatgaaagc gatcaaagaa tcatcctgat gctttcagga ggcttgatgg
     9841 ttcatctcgg cttgatatta ttggcgatag tgtttggatt tttgacaaga agctggtatt
     9901 ttgcagggac ttggattttt cttaatttat ctttcttcct aaataacatt ttgccagtcg
     9961 acatcaccga tggagcaaaa atttgggaat tactacaaca ccctgaaaat accaaatacg
    10021 cctacctggt attgagacat tctgctcaaa ctttgctggc tcctcaagaa tatgatttga
    10081 aagactttgt tcaagcagtt cctgaggatg cacgagggag ttttgcggat ggtgtattag
    10141 gtatgcaagg tgaagtttcc attttggaag ggaaggaaga agtagctaaa cagcagtttc
    10201 aagcattact tgagaggaca actactccga tgatgcagac agttgcccaa ctatccctgc
    10261 ttcatatcgc cttgttggaa ggagattttg agcaagcgga gcaatatgct agtattcgac
    10321 aggtcaaatc gtttctgtct ctcaaactgt ccaatcttca agtgatacaa gcttggtatc
    10381 aatttaaggt aaagaaagat gtagatcgaa ctcgtaaggc tctcaagatt gctagacaga
    10441 aaatggatag tagtcgaatg ttgagggacg agaaacgcta ttatgaaaac ttgttagcag
    10501 agctggaaaa ggaaatgtta gaaggaactg aagatggaac tagaaatttc tgatttccca
    10561 ggctgtaaga ttgctttgtt ttgtggggat aagctcttga ctatcttacg tgatgataag
    10621 gcaagtattc cctgggccaa tatgtgggaa ctgccaggtg gtggacgtga aggtgatgaa
    10681 agtccttttg agtgtgtagc gcgtgaagtt tatgaagaac tgggcattca tctgactgaa
    10741 gattgtctgc tttggagtaa ggtttaccca agtatgcttt ttgcagacaa acagtccgtt
    10801 tttctagttg gtcagttgac acagaaccag tttgacagta tcgtatttgg agatgaagga
    10861 cagggctatc agctgatgaa tgttgaggaa tttcttagtt ccagtcaagt tgtgcctcag
    10921 ttgcaagaga gattaaaaga ttatttaaaa gtaagtgatt agaaaggatg gttcagttaa
    10981 atttctaaac tgaacccgcc ctaaacactg tgccaaaaag ataaacttct cttagacaca
    11041 aacgtcttca gagaatttcc tattttggtt ttgtgtttta cgggcttggt atcttaatga
    11101 tggaaacatg gcaagagtta aaagttacag tgaagcgtga gggagaggag ttggtttcca
    11161 atctcttgat tgagctggga gcgcaaggtg ttgcgattga agacagtctg gactatgtgg
    11221 gaaatgttga ccgcttcggc gagattttcc cagaggtcga gcagcaagaa gaaatcgtag
    11281 tgacagccta ctaccctgac acggtggatg tgacagtggt tgaggcggac ttgcaggctc
    11341 gcatatcaga attgacggat tttatggatt tgggagagtt aaaaataggc acgactgcct
    11401 tggctgagga agactgggct gacaactgga agaagtatta tgaaccggca cgcatcaccc
    11461 atgacctgac catcgtaccg tcatggacgg actatgaggc aacagcagga gaaaagatta
    11521 tcaagctgga tcctggtatg gcctttggga cagggaccca cccaacaacc aagatgagcc
    11581 tctttgcctt ggaacaggtt cttcgtggtg gcgaaacagt gctagatgta gggactggtt
    11641 caggggttct ttctattgca agctcactcc taggtgccaa ggagattttc gcctatgacc
    11701 tggatgatgt ggcggttcgt gtcgctcagg aaaatattga gctcaacact ggtatggaaa
    11761 atatccatgt agcagcagga gacttgctta agggagttga gattgaagca gatgtcatcg
    11821 tggctaatat cttggcggat attctcattc atctgacgga ggatgcttat cgcttggtaa
    11881 aggatgaagg ctacctcatc atgagtggca ttatcaagga caagtgggac atggtgcgcg
    11941 agtcggctga gtcagctgga tttttccttg aaacccacat ggttcaaggg gaatggaatg
    12001 cctgtgtctt taagaaaacc aaggatattt ctggtgtgat tggaggctag gatgcaacag
    12061 tattttgtca aaggcagtgc tatctctcct gtcaccatcg aggacaagga aaccagtaag
    12121 catatgtttc aggttatgcg cttgaaagaa gaggatgagg ttaccttagt ctttgatgat
    12181 ggcatcaagc gcttggcgcg cgtgctggat atggaaaatc gtcagtttga gttggtccaa
    12241 gaattaaatg acaatgtgga actaccagtc caagtgacca tcgcatctgg atttcccaag
    12301 ggagacaagc tggagttcat cgctcaaaaa gtaaccgaac tgggtgctag ccaaatctgg
    12361 gcctttcctg cagactggtc agttgccaag tgggatggca agaaattggg taaaaaagtt
    12421 gaaaaactag aaaaaattgc ccttggagca gccgagcaaa gcaagcgtaa tattgtacca
    12481 agtattaaac ttttcgagaa aaaagcagat tttctagctc agctggacca gtttgactct
    12541 atcatagtag cctatgaaga atcagctaaa gaaggagaag ccgctgcgct cttacaagcg
    12601 gtctctggtc ttgaaacagg agccaaactg ctctttatct ttggaccaga aggcggtctc
    12661 tcacctgcag aaatcgaaag ttttgaagct aagggagcag ttttggtagg cctaggtcct
    12721 cgtattttgc gagcagaaac agcaccgctt tacgccttat cagcccttag tgttttagta
    12781 gaattagaga aataagagga agaaaatgga acaaaaacac cgttcagaat ttccagagaa
    12841 ggaactctgg gacttaacag ccctatacca agaccgtgag gatttcttgc gtgcaatcga
    12901 gaaagctcgc gaagacatca accaatttag ccgtgactat aagggcaatc ttcacacttt
    12961 tgaggatttc gagaaggcct ttgcggaatt ggaacagatc tacattcaga tgagccatat
    13021 tggcaactat gcttttatgc ctcagacgac ggactatagc aatgacgaat ttgccaatat
    13081 tgcccaagct gggatggaat ttgaaacaga tgccagcgta gccttgacct tctttgacga
    13141 tgccttggtg gtagcagacg aggaagtctt ggaccgtttg ggtgaattgc ctcacttgac
    13201 ggcagctatt cgtcaggcca aaatcaaaaa agcccactat ctaggggctg atgtggagaa
    13261 ggccttgacc aatctcggtg aagttttcta tagtccgcag gacatttata ctaagatgcg
    13321 agctggggat tttgaaatgg ctgactttga agcccatggc aagacctata aaaacagctt
    13381 tgtcacctat gagaatttct accaaaatca cgaggatgct gaggttcgtg agaaatcctt
    13441 ccgttccttc tcagagggac ttcgtaagca ccaaaatacg gctgcagcag cctatctggc
    13501 tcaggtcaag tctgaaaaac tcttggctga tatgaaggga tacgactctg tctttgacta
    13561 tcttctagct gaacaagaag tggaccgtgt catgtttgac cgccagattg acctcatcat
    13621 gaaggacttt gcaccagtcg ctcagagata cctcaagcat gttgccaagg taaatggtct
    13681 tgaaaagatg acctttgcag actggaaatt ggacttggac agcgccctga atcctgaagt
    13741 gactattgac gatgcctatg atttggtcat gaagtcggta gaacctttgg ggcaagaata
    13801 ttgtcaggaa gttgctcgtt accaagaaga gcgctgggtg gactttgctg ctaacagtgg
    13861 caaggattcc ggtggttatg cggcggaccc atatcgcgta cacccttatg tactcatgag
    13921 ctggacaggc cgtttgagcg atgtctatac cttgattcat gaaatcgggc attctggtca
    13981 attcatcttt tcagacaatc atcaaagcta tttcaacgcc cacatgtcga cctattatgt
    14041 tgaagcaccg tcaaccttca atgaattgct actcagtgat tacttagaga accagtctaa
    14101 tgacccacgt caaaaacgct tcgctctggc tcatcgcttg acagacacct acttccataa
    14161 tttcatcacc cacctcttgg aagccacctt ccagcgtaag gtgtatacat tgattgaaga
    14221 aggggagacc tttggagcaa gcaagctcaa cagcattatg aaggaagttt tgacagattt
    14281 ttggggcgat gccattgaaa tcgacgacga tgcagctttg acttggatgc gccaagctca
    14341 ctactatatg ggcttgtata gttacactta ctcagcagga ctagttatct cgactgctgg
    14401 ttaccttcat ctaaaacatt ctgaaactgg agctgaagac tggctcaatc tccttaaatc
    14461 aggtggtagc aagacaccac ttgagtcagc catgattatc ggtgctgata tttcaacaga
    14521 caaaccactc cgtgatacca tccaattctt gtctgacaca gttgaccaga ttatctccta
    14581 tagtgctgag ttgggagagt aggggaatag aaaaattttt tagaatgatg tttatgggct
    14641 tgttaagcgt cattctattt gtcttagttg tagctatttt caaaaatgtc gttgaaggtc
    14701 gtgatgttaa atttgaactt actgtagaac tggtattgat agttatttac gtcattttat
    14761 atcagattgt atttaattga gaattcaata ttgagtcatt taagactgcc tttttgaaca
    14821 gttgctagaa atgtagaggt attgaccatg tatcaagatt tactaagaaa aatagcagaa
    14881 gaaaaaccaa attataacca ggaggaaatt cagtggttgc ttgatcattt gggagatcct
    14941 tctccagaaa ttcgtgatga tcttgttttt acaagctttg ctaaggagat tcaggaagag
    15001 ctatttacac aggaacaatt tcatttcatt gctgaggtgg tcttagctga tgtagggtta
    15061 gataaagaga ttgataaggt aggcctgtca acacttgaac gttcttttag ggcacttatt
    15121 tatgcaaatc tcttgtctgc agatgccaac cagcaatcgg ttttttatca ggaattaaat
    15181 gcaggatttc gtaatgacct ttcaaatcaa ggcttgcact atctttcaaa agaaaaggat
    15241 acaacaggtt tctcaagtca gtatggttgg gttcattctt ttgcacatgg agccgattta
    15301 ctgacagagg tggtttgtca tccagacttt cctataaaca gagttcatga agtatttaat
    15361 atacttggcc aactatttaa aagaatgtcg attcgcttta cagatgatga ggattggcgt
    15421 ttagcaagag tgatctacga acctatttta caagggaagt tagcgcaaga acaagtagct
    15481 tcttggataa aaactgttga ttttccgata gaagaaaggg aggattttta caaattttcc
    15541 aactttagat cctgtctggt ggaagtctat gtccaacttg accagagaaa tagtttacaa
    15601 gatgagttga aagaagctat ccagtctttt caatactaga gattagctaa tagttctatg
    15661 attttcaaaa aatttccaat caattttctt gaaacccttt cctacttttg atagactagt
    15721 atctagtttt tgaaaaaagg agaaagaaaa tgaaaaattt gttgctgagt taatcggtac
    15781 gttcatgctt gtgttcgttg gaacaggagc tgttgttttt ggaaatggtc ttgatggcct
    15841 tggtcacctt ggaatcgcct ttgcctttgg tttggcaatc gtggtggcag cctactcaat
    15901 cggaactgtt tcaggtgctc acttgaaccc ggctgtttcg attgctatgt ttgtaaacaa
    15961 acgtttgtca tcttcagaac ttgtaaacta catccttggt caggttgttg gagctttcat
    16021 cgcttctggc gcagtcttct tcctcttgag taattcaggt atgtcaactg ctagccttgg
    16081 tgaaaatgcc ttggcaaacg gtgtcactgt ctttggtggt ttcttgtttg aagttatcgc
    16141 aactttcttg tttgtcttag ttatcatgac tgtgacttca gaaagcaagg gcaatggcgc
    16201 gattgctggt ttggtaatcg gtttgtcatt gatggcgatg attcttgtcg gattgaagat
    16261 tactggactt tcagtaaacc cagctcgtag cttggcacca gctgtcttgg taggcggcgc
    16321 agcccttcaa caagtttgga tttttatcct tgcaccaatc gctggtggag ttcttgcagc
    16381 ccttgttgca aaaaatttcc ttggaacaga agaataattg aaactaaaaa agccttgctc
    16441 ctcatcttga ggaacagggc tttttcgtat gatactcttc gaaaatatct tcaaaccacg
    16501 tcagcttcac cttgccgtag gtatggttac tgacttcgtc agttctatct gcaacctcaa
    16561 aacagtgttt tgagctgact tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc
    16621 taacttcgtc agttctatct gcaacctcaa aacagtgttt tgagctgact tcgtcagttt
    16681 tatctacaac ctcaaacact gctttgagta acctgcggct aacttcctag tttgctcttt
    16741 gattttcatt gagtatgact ttagcggttg tcaattttct ctggataaag gtcgtgttgg
    16801 aagaggcgtt gttctgccaa gccctcatac ttagttcctt gcttaccgta gttgtagtag
    16861 gggtcgattg aaatgccacc gcgcggagtg aattttcccc agacttctaa atagcgaggg
    16921 tctagcaagt tgaccaagtc tttcccgatg gtgttgatac agttttcgtg gaaatctccg
    16981 tggtttcggt agctaaatag atatagtttg agggattttg actcgacaca gagcttgtca
    17041 ggaatgtagg aaatatgaat cgtcgcaaag tctggctgag cagtgatttg tcccagcaga
    17101 gacatatcga ggatatggtg acgaatgccc tgttccttag cgatttctct agtaatttga
    17161 atttcgaggt gatgacgttg gccgtaggca aaggtgacag cttcgactgt ttcatagtgt
    17221 tgcatggccc agaaaaggca ggttgttgaa tcttgaccac cactaaagac gaccaaggct
    17281 gattgatgtt tcatagtact ccttccaaaa tgggaaatgt tcagagcacg caaaaagctc
    17341 ccattaggga gctaaaaaat accaaatcga gtttttttag cgatggcata tcccaaacat
    17401 cgtaatattc tacttatata gtaaaatgaa acaaaaacag gacaaatcga tcaggacaat
    17461 caaatcgatt tctaacaatg ttttagaagt agagatgtac tattctagtt tcaatctact
    17521 atagtctagc atattttttg aaaaatggca aagggcaaga aaaaagagac caaagaaagt
    17581 acttggtctc tcgtttgatt agctcaattc agcaatgatg gccttgattt gttctgctgt
    17641 gtgaacacct gcaacttgtt tgacaacttg gccgtctttt ttgaagagaa gagttggaat
    17701 agacatgatt ccaaaagcac gagctgtgtt tggattttca tcaacgtcca ttttaacgat
    17761 tttcaagaca tcttctgaaa gttcttcaga caatttgtcc aagattggac cttgcatacg
    17821 acatggacca caccaagttg cccagaagtc tactaagacc aaaccgtctt ttgtttcttg
    17881 ttcgaatgtt gcatctgtaa ttgcttttgc cattgtattt ctcctttttt tagttatatt
    17941 ggcttaaatc ttgtttcatg agatagaaga agatatctcc ataagtccca tggtagtcca
    18001 aattatgacc cttgtaagtt aatttttgga cagggtagta gtctgcgacg ccgataaggc
    18061 aagcttgttg cgaacgttca aagtcttcat aagactcgaa agtcacagtt ctttcgttct
    18121 tgctggcatc tatataggta atttcaatca tgtttaaaac tcctttgttt aatgctaact
    18181 ttattttact ccttataaaa gagaatgtca agaaaaatga ttgcgcacgc aacttttttt
    18241 aaaatcatct taaatcaaga aatccaaacc tgcttccaag ctttcttcga cagtcttttg
    18301 tagcgaggcc agtgtctttt gcccatcatt tgtcaggcag ataaaactag agcgtctatc
    18361 ttgatggcaa cacatgcgac tgagtagacc gcaattttta gcttccaagc gagccaccat
    18421 cctagaaact gcgctcgggc tcagatgaag cttatctggc aggtcaatct ggcgtagaga
    18481 tttttcttca gccaagtcca gatagtagag caggtagaac tctttcaagg tcagactttg
    18541 ctcgctctgt tgggcaatgg tctcttccaa gagactttca atttctttct gacgccgatt
    18601 gaagtcaaac catttttcca aataggtcat agtgtctcct ttctttttag agtcataatt
    18661 atagaagaaa gtccattaac gggcagtctt tgcgtcataa gatgattgcg catgcaataa
    18721 ttatactact tttcaagaat gctggcaagc tctgtttttt agtggttttc ttttttactg
    18781 tctatatttt tggtaaaaat caacttttac ttggatgaag gttttggctt cacgtaggag
    18841 ttgaagaaga gtggcgcggg tttcaaactc ttctcttgtc ttgggcagac tgcggttccg
    18901 gaagacttcc agataacgtt caatttcatc tagcaaatca gaagcaggat tggtctggct
    18961 cagttgacct gcaatttttg aaaagagttg cgctaagatc aggctttcac tggcggcaag
    19021 gtgacaagtg ttaatctgtt gggccatgtt tctcaggata cgactttgtc gttgtctcat
    19081 ctcaaagtag tggatatggt agtcggtctg gtgaaagagg tggtcagagt gatccaaata
    19141 gaccagtctg agggcttctt tcaaaagcgt gtctaattct gctaccagct gtgctcggtt
    19201 gcgtccgtct cctctggata aatagtattt gaagcgctgg aggatatctt ttaacttttc
    19261 ttccaccagc gtgtggtagt gctggatttc ctcttctcgt gaaggcatat agagattaac
    19321 aagcaaggca aatcctgtac caatagcaaa gagaaggaat tcgttgacta ggaggtctgg
    19381 agaggttgac tcttgaacca agagatggct aaccaaaacc gtgcttggtg tgatgccaat
    19441 ttcccagccc atcttgtagg ctaaaggaac gtagaaggcc agatagaggc cgagactcca
    19501 gatatgaaat ccgctcaagt gaaaagctag aacgccgata gccagagcta gaagcataga
    19561 aaaaagacga ttgcgagcca gttttaaagt acgtctacgc gtatcagata ggctcaagag
    19621 agcgataatt ccagccgaaa ctgctgacga aagattgaga aaataagcaa gcaggcaggc
    19681 aagacaggta gctaagatga gcttggtcgt acgttggcta atagacataa gaatttccta
    19741 ataagttaga ataaaagcgt aaaagacaag acatgagcag gcttgccttg atgagttatt
    19801 ttttacgggt tgctgcgtat tcggcaacgg cggtaaagag gacatctgta gaagagttaa
    19861 gggctgtttc acatgagtct tggatgacac caatcacaaa accaacccca acaatttgta
    19921 tggcaatatc gttagaaata ccgaaaaggc tacaagcaac tgggataaga aggagggaac
    19981 ctccggcaat acctgaagca tcacaggctg agatagctgc taccacactg aggacaaagg
    20041 ctgtggcaaa gtcaacagga attccaagag tgttaactgt aacaagggtc aaaacgttaa
    20101 tggtaatcgc tactccagcc atattgatag tagaaccgag tgggatagaa acagaatagg
    20161 tatctgggtt gagtccaagg tcatggcaga gtttcatgtt gacaggaatg ttagtcgcag
    20221 aactacgagt gaaaaaggct gtcacaccgc tgacacggag gcagttccaa actagagggt
    20281 aaggattgcg tctcataaag aagaaggcaa tcaaagggtt gaccacaggg gcaacaaaaa
    20341 gcatagtcgt tactaataga accaataaaa taccgtagtt ggcaaggctt ccgactccct
    20401 tgtcagaaat ggttttaaaa acaagaccaa ggattccaaa tggagccaga ttgatgatcc
    20461 attcgacaat tttagaagtc acgtcagcga tagtttttag caattcttta ctatttttac
    20521 tggcttctct catagcgatt ccaaaaatga ctgcccaaga taagattcta atatagttag
    20581 cagtaagcag ggcgttgact gggttgtcaa ccagtttgag caagaggttg ctgaggacct
    20641 gcccaatccc atctggtggt gcaatttcag tattggcact atttagggta atttcaatag
    20701 ggacgatgaa acttgctagt acagctacaa gagcagcggc gaaagtccct atcataggat
    20761 atacaagaaa acaacagttt tcatattgct atcttgtccc ttttgatgtt ggaaagggca
    20821 ttggcaacga gggcaaagac taggatagga gcaacagctt ttagacctcc aacgaataaa
    20881 tccccgagta gcccaatccc tgagagatta ggaagggtca gtcctaggat tccccacaaa
    20941 gcataccaat caagatacgc ttgacaaggc ttgccttatt ccaagcatga atgattcttt
    21001 tcataataat ctcctttttg tgtagtgatt atgattatag tataaatgat agacaaaatc
    21061 aagaattttc tgtctatttt ttgaatattt atggagaatg agactgatga aaatatggta
    21121 taatgaaata aaggagtttt atatgcaaaa atttattcag gcttatattg aaaagctaga
    21181 tgtgacaacc attatcgaga atattctaac caaggtcatt tctcttttac tgcttttgat
    21241 tgtattttat attgctaaaa aaatgcttca taccatggtg cagagaattg tcaaaccttc
    21301 tctaaaaatg tctcgtcatg atgtcggacg ccaaaaaacc atctcacgtt tactagaaaa
    21361 tgtgtttaat tatacgctat atttcttttt actctactgc attttgtcga ttttaggttt
    21421 gccagtttct agtttgctgg ctggagctgg tattgctggg gtagcgattg gtatgggagc
    21481 ccaaggcttt ctgtctgatg tcatcaatgg ctttttcatc ctctttgaac gtcaactgga
    21541 tgtgggagat gaggtcgttc tgacaaatgg accgattact gtatcgggta aggttgtcag
    21601 tgtgggaatt cgtacgacac agcttcgtag cgaggagcaa gcccttcact ttgtccctaa
    21661 ccgaaatatc acagttgtta gcaatttctc acgcacagac tagacctgtt attttaagta
    21721 atttgtggta caatagaggg agtttaataa ggagaaaaga tggttttaga aaagcagttg
    21781 ggcaatggtt gtacctggat agacctagac ctaggaaagt tgaataaact agaagacctt
    21841 tctgaaattt acggtttgga caaggaaacc attgaatacg cactggatag aaacgagcgc
    21901 gcccacatgg attaccaccg tgaaagtggg acggttacct ttatctataa tgtcctagac
    21961 gtaaaaaagg acaaggccta ctatgagact tttcccatga cctttattgt cgagcatcgt
    22021 cgcctgatta ccattagtaa taccaaaaac gcctatgtca ttgaacagat gactcgttat
    22081 ctggagagcc atgacacgct ttcgatttat aagtttctct ttgccagtct ggaaattatc
    22141 agcaatgcct actatcctgt cattgagcag atggacaaga gtagggatga ggtcaatgac
    22201 ctcttgcgcc agcgaactac caagaaaaac ctctttgccc tgtctgattt ggagactggt
    22261 atggtttatc tgacggcagc tgccaaacaa aatcggattt tgttagagca tattcaaggt
    22321 catgccttgt atcgtagttt tgatgagatt gagagagaac agtttgatga tgccatgatt
    22381 gaggctcatc agctggtatc catgacagac ctaatctctc agattttaca gcagctttca
    22441 gcctcttaca acaatattct aaacaataat ctgaatgaca atttgacaac cttgactatc
    22501 atttcagtct tgctagctgt tttggcagtc gtgacaggct ttttcggaat gaatgttccc
    22561 ttacctttaa cagatgagcc ccatgcttgg ctctatatca gtttggctag tgcaggtttg
    22621 tggattgttt tatccttgtt actaaggaaa attgcgaaaa aaagttaaga aaaggagcca
    22681 gaatggcgat tgaaaattat ataccagatt ttgctgtgga agcagtctat gatctgacag
    22741 tcccaagcct gcaggcgcag ggaatcaagg ctgttttggt cgatttggat aataccctca
    22801 ttgcttggaa caaccctgat ggaacgccag agatgaagca atggctacat gaccttcggg
    22861 acgcgggtat tggcattatc gtagtgtcaa ataacaccaa aaaacgcgtt caacgagcag
    22921 ttgagaaatt tgggattgat tacgtttact gggccttgaa gcccttcaca tttggtattg
    22981 accgtgctat gaaggaattc cactatgaca aaaaggaagt ggtcatggtt ggcgaccagc
    23041 tcatgacaga tatacgagca gcccaccgtg cagggattcg gtcaatttta gtcaaaccct
    23101 tggtccaaca tgactcaatc aaaacgcaga ttaaccgaac tcgtgagcgt cgtgttatga
    23161 gaaaaatcac tgaaaagtac ggaccgatta catataaaaa aggaatttaa ctatggaaga
    23221 aattctctgt attggttgtg gagcaaccat tcagacgaca gataaagctg gtcttggttt
    23281 taccccccag tcggcacttg aaaaaggttt ggagactggc gaagtctatt gccaacgctg
    23341 tttccgtctc cgccactaca atgaaatcac agatgtccag ttgacgaacg atgatttcct
    23401 caagctcttg cacgaggtgg gagacagtga tgccttagtg gtcaatgtca ttgatatctt
    23461 tgattttaat ggatctgtca tcccaggttt accacgtttc gtctcgggca atgatgtcct
    23521 cttggtagga aataaaaaag atatccttcc taagtcagtt aagtctggta agattagcca
    23581 gtggctcatg aaacgtgccc atgaagaagg tcttcgtcca gtcgatgtgg tcctaacttc
    23641 agcacaaaat aaacatgcca ttaaggaagt cattgacaag attgaacact accgtaaggg
    23701 ccgcgatgtc tatgtggtcg gtgtgaccaa cgttggaaaa tcaactctaa tcaatgctat
    23761 tatccaagaa atcacgggtg atcagaatgt catcactact tcacgcttcc caggtacaac
    23821 cttggacaaa atagagattc cgcttgacga cggatcttat atttacgata cgccgggaat
    23881 tatccaccgc caccagatgg ctcactactt gacggccaaa aacctcaagt atgtcagtcc
    23941 taaaaaggaa atcaagccta agacctatca gcttaatcct gagcaaaccc tatttttagg
    24001 tggtttggga cgctttgact ttatagcagg agaaaagcaa ggatttactg ctttctttga
    24061 taatgaactc aaactccatc gtagcaagct tgaaggagct agtgctttct acgataagca
    24121 cctgggaact cttctgacac caccaaatag caaggaaaaa gaagatttcc caaggctagt
    24181 ccagcatgtc tttaccatta aagataagac agacctagtc atctcaggcc taggctggat
    24241 tcgtgtaaca ggcatagcaa aagtcgccgt ctgggcacca gaaggcgtcg ccgtcgtcac
    24301 acgaaaagca attatttaaa acaaaatcgc tgagcgaaca aagtgagctc atatagcgat
    24361 agttattgcc gtggtgtgat tgccaatctg tagattggca aggggcacaa ttgagaccaa
    24421 ggctcaattg tgaggtcagg aaatccattt ttcaaagatg aaatctttgg aaaattagtt
    24481 ccgaccgtcc ctcaccacct aaaataacta tccaaaaaca aggaagaaaa ttatgtcatt
    24541 aacatcaaaa caacgtgcct tcctcaacag ccaggcacac accctcaaac ctatcatcca
    24601 aatcgggaaa aatggactca acgaccaaat caaaaccagc gtccgtcaag ctcttgatgc
    24661 gcgtgaatta atcaaggtta ctctcttaca aaacacagat gaaaatatcc acgaagtagc
    24721 tgaaattttg gaagaagaaa tcggtgtgga tacagtccaa aaaataggac gcatcttgat
    24781 tttgtttaaa caatctagca agaaagaaaa tcgcaagatt tctaagaaag tcaaagaaat
    24841 ctaagaccga aactccaaat aactgttttt atagagaaat aaaggggact agcctatgac
    24901 aatcgaacta ttgactccct ttaccaaggt agagttggag ccagaaatca aggagaaaaa
    24961 acgcaaacaa gttgggattt taggggggaa ttttaaccct gttcacaatg cccatctcat
    25021 tgttgcggat caagtacggc aacagttggg actggatcaa gttcttctca tgcctgaata
    25081 ccaacctcct cacgttgata aaaaggaaac catccctgaa caccatcgtc tcaagatgct
    25141 tgagttggca attgagggga ttgacggcct agtcattgaa accattgagt tggagcgcaa
    25201 gggtatttcc tacacctacg acaccatgaa aatcttgaca gagcaacatc cagatacgga
    25261 ttattacttt atcatcggtg ccgacatggt tgactatctg cctaagtggt accgaattga
    25321 tgaactggtt gacatggttc agtttgtggg ggttcagcgt ccacgctaca aggcagggac
    25381 ttcctatcca gttatctggg tggacgtacc gctcatggat atctcgtcca gcatggtgcg
    25441 ggacttcatt gcccaaggtc ggaaacccaa ctttctccta cctcagccag tgctagacta
    25501 catcgagaag gaggggctct actgatgtcc tatcaagact atatcaactg ctcccgtgag
    25561 gccttgttgg aaaaaatggc agagcttcta cctgagaaac gtctaaccca ttgtctaggt
    25621 gtggagcgtg ccgctatgga gttagctcag cgctttggtg tagatgttgg gaaagcaagt
    25681 ctagcaggtc ttcttcatga ctatgctaaa aagttgtcag atcaggaatt tctagacttg
    25741 attgaccgtt atcagctaga tttcgacctc aaaaactggg gtaataatgt ctggcatggt
    25801 atggtcggaa tttacaaaat tcaggaagat ttggatttgc atgattcaga aatcctgcga
    25861 gccattgaag tccatacagt cggtgctggt caaatgacag acctagataa ggtcatctac
    25921 gtcgcagact atatcgagca caatcgagcc tttcctggag tagatgtggc gcgtgagatt
    25981 gcaagtctat cgctcaataa ggcggtggcc tacgaaacag ctcgtaccgt ggagtatcta
    26041 gctcatcagg gattccccat ctatccccaa acccttgaaa cctataacgc ctttgtgcac
    26101 tatttgaaag aggactaaat gaagacggct tttataatca ttgatgttca gaatgtttta
    26161 gtagaaactg ggttccagac aaaaagtcta ttggaaaaaa tttcttattt acaaaaccag
    26221 gctagaagca agaatattga aattatctat gttcaacata ttgagaattc tgaagctcaa
    26281 acatcagaag attggcagtt atctgcgctt ttaaatcgaa aacctgctga aaaggtcttt
    26341 cagaagaagt ataacagtat tttcaaagaa actggcttaa aagaatactt ggataaacag
    26401 gggattgaaa aattagtttt atgtggtatg cagacagaat attgtgtgga tacctctgtc
    26461 aaggttgcct ttgaatatgg ctatcagctt attgttccag aaggtgctgt cacaaccttt
    26521 gatggggatg acattccagc agaaacgatt aatgaatttt atgaggacat ttgggaggag
    26581 cactttgcag atgtcctaga ttacaaacat attttctaaa aagaggatta aatgaacgaa
    26641 aaagaattac tagaactagt cgtgaaagcg gctgatgaga aacgtgcgga ggatatcctc
    26701 gcacttgatg tacaagattt gactagtgtg acggactact ttgtcatcac tagctcaatg
    26761 aatagccgtc agttggacgc tatcgcagct aatatccgtg aaaaagtagc tcaagcgggc
    26821 tttaaaggta gccatgtcga aggcgatgca gctggaggct gggtcttact ggacctcggt
    26881 gctgttgtcg tgcatatctt ttcagaagaa atgcgtgccc attataatct agagaagcta
    26941 tggcatgagg cgaattcagt agatatttca gaagctcttg cttagaagat gaactcagtt
    27001 gtagtcaact gggtttcttt gcttatttta gaaaaattga tagaaaggta aagggctagt
    27061 ggtgtttacc atggagctct tagtatcatg attatggcga cttatgaaac ctttgcggcg
    27121 gtctatgatg cggtcatgga cgatagttta tacgacaaat ggacaaattt ttctctgcgt
    27181 catttgccta agaccaagga gaaaaagaaa ctcttggaac tggcttgtgg gacaggaatt
    27241 caatcagtgc gcttctctca ggctggtttt gatgtgactg gacttgactt gagtgcggat
    27301 atgttgaaga ttgcggagaa gagagcaact tcagccaagc aaaagattgc ttttatagaa
    27361 ggtaatatgc taaatttgtc caaggcaggg aaatacgatt ttgtcacgtg ttattcggac
    27421 tctatctgct atatgcagga tgaggtggaa gtaggggacg tctttaagga cgtgtacaat
    27481 gcgctgaatg aagagggagt tttcatcttt gacgtgcact cgacctacca gacagatgaa
    27541 gtattccctg gctattccta ccatgaaaat gcggaagatt tttccatgct ttgggataca
    27601 tatgaggacg aagttcctca ctccatcgtg catgagctga ctttctttat caaggaggct
    27661 gacggttcct ttagtcgcca cgatgaagta catgaggaga ggacctatga gatcttgacc
    27721 tatgatatct tgctggaaca ggctggattc aagtccttca aactctatgc agactttgag
    27781 gataaggagc caacagaaac tagcacccgt tggttttttg tggcgcagaa gtaggagatt
    27841 agcatgacca tcacaggtat tatcgcggag ttcaatcctt ttcataatgg ccataaatac
    27901 ctgctggatc aggcagaggg actgaaaatt gttgccatgt cagggaattt catgcagcgt
    27961 ggagagcccg ctattgtgga taagtggaca agggcccaga tggcgctgga aaatggcgca
    28021 gacttggtag tggaattgcc ctttttagtc agtgttcagg cagctgattt ctttggtcaa
    28081 ggagctgtgg atatcttgga tcgcttgggc attgatagtc tagtttttgg gacagaggaa
    28141 gttcgagatt atcagaaaat cgctgactta tacacagaga aaggtgctga gatggagaaa
    28201 tttgtggaaa atctgcctga ttccctttcc tatccccaga aaacccaagc tatgtggaag
    28261 gaatttgctg gtcttgattt ttcaggtaat acccccaatc atgttcttgc tctggcctat
    28321 gcaaaggcgg ttgcaggacg aaatatcaag ctgcatccga ttcagcgtca gggggcaggt
    28381 taccattctg tgaataaaga tgtggacttt gcctcggcga cagccctccg tcaacatcag
    28441 aaggaccaag atttcttaga acgttttatg ccttctgttg ccctatttga gcaggccagt
    28501 aaggtgatct gggaagacta ttttcccttg ctccgctatc aaatcttgtc aaatcccgac
    28561 ctaaccacca tctatcaggt caatcaagaa atggctgtgc gcattaaaga agctattaaa
    28621 acagcccagt ctgtggaaga attggtcgag ttagtgacca ccaaacgtta caccaaggcg
    28681 cgtgtcagac gcctcttgac ctatatcttg gtgcaggcta gagaaagtga cttgccagaa
    28741 gggattcatg tccttggctt taccgaaaaa ggcaggcaac atcttaaata tctgaaaggg
    28801 caggtcagtc tagtcagcag aattggcaag gaaccttggg atgtcatgac tcaaaaggca
    28861 gaccagattt accaattggg aaatccaagt atagcagaac agaattttgg cagagtgccg
    28921 attagaatag aaacaaacta agtctactga aaggtaggct tttttattgt agaaatttta
    28981 gctaacaaat tgaagataaa gtttttggaa aatgataaac tccctaatac agaagacaaa
    29041 aagtcaataa agaaaattag tatatgaacc ttaatcttta tctttgaatc cgctttatgc
    29101 ttgttatt
//
LOCUS       DAVKWS010000023        26078 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKWS010000023 DAVKWS010000000
VERSION     DAVKWS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.023"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            369..1532
                     /locus_tag="VW852_001087"
     CDS             369..1532
                     /locus_tag="VW852_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV0272245.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGNYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLTMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1529..2206
                     /locus_tag="VW852_001088"
     CDS             1529..2206
                     /locus_tag="VW852_001088"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV0272246.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2196..3050
                     /locus_tag="VW852_001089"
     CDS             2196..3050
                     /locus_tag="VW852_001089"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV0272247.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3069..4136
                     /locus_tag="VW852_001090"
     CDS             3069..4136
                     /locus_tag="VW852_001090"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV0272248.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4146..5312
                     /gene="aroC"
                     /locus_tag="VW852_001091"
     CDS             4146..5312
                     /gene="aroC"
                     /locus_tag="VW852_001091"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV0272249.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5322..6425
                     /locus_tag="VW852_001092"
     CDS             5322..6425
                     /locus_tag="VW852_001092"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV0272250.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6436..6774
                     /locus_tag="VW852_001093"
     CDS             6436..6774
                     /locus_tag="VW852_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV0272251.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6866..8149
                     /gene="aroA"
                     /locus_tag="VW852_001094"
     CDS             6866..8149
                     /gene="aroA"
                     /locus_tag="VW852_001094"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV0272252.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8142..8618
                     /locus_tag="VW852_001095"
     CDS             8142..8618
                     /locus_tag="VW852_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV0272253.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQRDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8615..9463
                     /gene="pheA"
                     /locus_tag="VW852_001096"
     CDS             8615..9463
                     /gene="pheA"
                     /locus_tag="VW852_001096"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0272254.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9460..10734
                     /gene="psr"
                     /locus_tag="VW852_001097"
     CDS             9460..10734
                     /gene="psr"
                     /locus_tag="VW852_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV0272255.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11013..11858
                     /locus_tag="VW852_001098"
     CDS             11013..11858
                     /locus_tag="VW852_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0272256.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFFPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11848..12951
                     /locus_tag="VW852_001099"
     CDS             11848..12951
                     /locus_tag="VW852_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0272257.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12953..13939
                     /locus_tag="VW852_001100"
     CDS             12953..13939
                     /locus_tag="VW852_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0272258.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDARVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13955..15388
                     /locus_tag="VW852_001101"
     CDS             13955..15388
                     /locus_tag="VW852_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV0272259.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15417..16451
                     /locus_tag="VW852_001102"
     CDS             15417..16451
                     /locus_tag="VW852_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0272260.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16651..17388
                     /gene="mecA"
                     /locus_tag="VW852_001103"
     CDS             16651..17388
                     /gene="mecA"
                     /locus_tag="VW852_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV0272261.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17539..18825
                     /locus_tag="VW852_001104"
     CDS             17539..18825
                     /locus_tag="VW852_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV0272262.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18827..19696
                     /locus_tag="VW852_001105"
     CDS             18827..19696
                     /locus_tag="VW852_001105"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV0272263.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19946..20884
                     /gene="msrB"
                     /locus_tag="VW852_001106"
     CDS             19946..20884
                     /gene="msrB"
                     /locus_tag="VW852_001106"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0272264.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20942..22666
                     /locus_tag="VW852_001107"
     CDS             20942..22666
                     /locus_tag="VW852_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272265.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGTRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLITSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22668..24416
                     /locus_tag="VW852_001108"
     CDS             22668..24416
                     /locus_tag="VW852_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272266.1"
                     /translation="MKRQTVNQTLKRLVVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24808..26067
                     /locus_tag="VW852_001109"
     CDS             24808..26067
                     /locus_tag="VW852_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV0272267.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
BASE COUNT         7680 a         4904 c         5893 g         7601 t
ORIGIN      
        1 atattgagtt tgtaacaaaa ggcttctcta taaaatcaaa ttttccagat atctactgaa
       61 gctgatttat ttcttcatct ttcttctact acctttgtca ctaaatttga cagacacata
      121 ttgctgatac aacctctcta cctataatct cacagttaac ctgaggctag tttcatagtt
      181 tgctatttga ttttcgttga taaaaaaaat gctcctccaa gacggaagag cattttttat
      241 tgtgattctt acacatttac ttcctagttt tgcaggaagt ctagtatacc attttttaaa
      301 agaagagtaa aaaagcaagg gtagtttctt ttttgagaat tgtctaaaag tttgatataa
      361 tggtagttat gaatagaata agagtcagca aaagggttga aaagaagctt gctaaggggc
      421 tagttttact agaagccagt gatcttgaga atgtcaatct taaggatcag gaagtagagg
      481 tgcagggtca ggaaggaaac tttcttggga ctgcctacct ttctcagcaa aacaagggct
      541 tgggctggtt tgtcagcaaa gacaaggtgg ccttcaatca agctttcttt gaaacgttgt
      601 ttagaaaagc caaagaaaag agaagcgcct actatcaaga tgatttaaca actacctatc
      661 gtctctttaa tcaagaggga gatggctttg ggggtctgac agtggacctt tatggcaact
      721 acgccgtctt ttcttggtat aactcttatg tttatcagat tcgtcagact atatcagaag
      781 cctttagaca ggttttccct gaggttttag gggcttatga gaaaatccgc tttaaaggtt
      841 tggactatga atctgcccat gtttatggtc aagaagcacc tgactttttc actgttttag
      901 aaaatggtgt cctgtatcaa gtctttatga atgatggctt gatgacagga attttcctag
      961 accagcatga ggttcgcggt agtttagttg acggcttgac tatgggtaaa tccttgctca
     1021 atatgttttc ctacacagcg gctttttcag tagctgcggc catgggagga gctagccaga
     1081 caacttctgt tgatctagcc aaacgttcac gagaattgtc tcaagcgcat tttcaggcaa
     1141 atgggctcag cacagacgag catcgtttta tagtcatgga tgtctttgag tatttcaaat
     1201 atgccaaacg caaagacttg acctacgatg tgattgtcct agatccgcct agctttgctc
     1261 ggaataaaaa acaaactttc tctgtggcca aggattatca caagttgatt tcccagagtc
     1321 ttgagatttt aaatccggga gggattatca ttgccagtac caatgctgcc aatgtttccc
     1381 gtcagaaatt tacagaacaa attgataaag gctttgcagg aagaagttac cagattttaa
     1441 acaaatatgg tcttccagca gattttgcct ataataaaaa agatgaaagt agtaattacc
     1501 tcaaggtgat tagtatgaag gttagtaaat gaaattaatc gtttcagtaa tgccaagaag
     1561 tttagaggag gctcaggctc tggatgccac gaggtacctg gatgccgaca tcattgaatg
     1621 gcgtgccgac tatctgccta aagaagcgat tttgcaggta gctccagcca tttttgaaaa
     1681 attcgcaggc cgtgagttgg ttttcacgct acgaactcgc tccgaaggtg gagaaatcga
     1741 cctttctcca gaagagtata tccatctaat caaggaagtt gcgcaattct atcaaccaga
     1801 ctatattgat tttgagtact atagctacaa ggatgttttt gaggaaatgc tggacttccc
     1861 aaatcttgtt ttgagttacc ataatttcca agaaacacct gagaacatga tggaaatctt
     1921 gtcagagttg acgatcctaa atccaaaact tgttaaggtt gcggtgatgg ctcacacgga
     1981 gcaggatgtc ttagacttga tgaactatac acgaggcttt aaaaccctca atcctgaaca
     2041 ggaatatgtg accatttcta tgggtaaggt gggcaaggtc tctcgtatca ctgcggatgt
     2101 gaccggttcg agttggtctt ttgccagtct ggatgaggtt agtgcccctg ggcagatttc
     2161 tctagctagc atgaaaaaaa tcagggagat tttggatgaa gcttgatggc tatacacgtt
     2221 tagctgcagt tgttgccaat cctattaagc attctatttc tcccttcatc cacaatagag
     2281 cctttgaggc gacagctacc aacggtgctt atgtggcttg ggagattgaa gcgagtgact
     2341 tggcagaaac agtggccaat attcgtcgct accagatgtt tggcatcaat ctgtccatgc
     2401 cctataagga gcaggtgatt ccttatttgg ataagctgag cgatgaagcg cgcttgattg
     2461 gtgcggttaa tacggttgtc aatgagaatg gcaatttaat tggatataat acagatggca
     2521 agggattttt taagtgcttg ccttctttta caatttcagg taaaaagatg accctgctgg
     2581 gtgcaggtgg tgcggctaaa tcaatcttgg cacaggctat tttggatggc gtcagtcaga
     2641 tttcggtctt tgttcgttcc gtttctatgg aaaaaacaag accttaccta gacaagttac
     2701 aggagcagac aggttttaaa gtggatttgt gtgctttaga gtatgtttct gaactgcaag
     2761 caaggattgc cgagtcggat ttgctagtta atgccaccag tgtgggcatg gatggccaat
     2821 cctctcctgt tcctgaaaac atagtcttac cagaaactct cttagttgca gatatcatat
     2881 accaaccctt tgaaacaccg tttttgaaat gggctagaag tcagggcaat ccagcagtca
     2941 atggtctggg aatgttgctc tatcaagctg cagaagcttt tcaactgtgg acaggcaagg
     3001 aaatgccgac agaagagatt tggcagtctt taacagaaaa ataccaataa agaaagggag
     3061 actctcctat gaaaatcaga atcgatattc ctcatcatcc ttatgatatt cagattgaaa
     3121 aaggttgtat ggcccaggct ggtcagtggt tgcgagaact ctggcaaccg caaaaggtag
     3181 tcattgtgac agataaccat gtagcctctc tctatgcaga gaaggtcaag ctcagcctag
     3241 aagatgctgg ttttcaggta gctgtttttg atttcttaga aggtgaagaa agaaagaatt
     3301 taactactgt tcagaaagtc tatgaatttc tagtcaagca aggtctgact cgtagcgatg
     3361 gaatcgttgc tcttggtggt ggcgttgttg gggacctggc tggatttgta gcctctacct
     3421 atatgcgggg tattcacttt gttcagattc cgactagttt gacagctcag gttgattctt
     3481 ctatcggtgg aaagacaggc gttaacacac catttgctaa aaatatggtg gggacctttg
     3541 cccaaccaga tggggttctg attgatccac ttgttcttga aaccctcgga aaaagagagt
     3601 tgattgaagg gatgggtgaa gttatcaagt atggcttgat tgaggatcca gaactgtggg
     3661 ctctcttgac ggggctgaat ggttctgttg agagcatttt ggaacatgca gagaccttga
     3721 ttgaacattc ttgtcaggtg aagcgcaaga tggtggttga agatgagttg gacaatggta
     3781 ttcgtcttta cctcaatttt ggccacacta ttggccatgc catcgaagcg actgccggtt
     3841 atggcaaggt catgcatgga gaggctgttg ccatgggaat ggtacagatt tccaagattg
     3901 ctgaggaaaa aggcctcatg ccagctggca ttacccaatc tatcacagag atgtgtcaga
     3961 aattcggctt gcctgttgac tatgaaaatt gggaagttga caagctttat caggctctta
     4021 ctcatgacaa gaaagcgcgt ggtaacacct tgaaattggt cttggtgcca gagcttggtt
     4081 cagcgaccat tcatccagtt tctctggaag agatgaaaga ctacttggta aaataaggag
     4141 agtgtatgag atatttaact gcaggagaat cacacggccc ccgtctaaca gctattattg
     4201 agggaattcc agctggactt ccattgacag ctgaggatat caatgaggac cttagacgcc
     4261 gtcagggtgg ctacggtcgt ggtggtcgta tgaagattga gagtgaccag gttgtcttta
     4321 cttcgggcgt tcgccatggg aagacgacag gggcgcctat tactatggat gtcatcaata
     4381 aggaccacca gaaatggctg gacatcatgt ctgcggagga cattgaagac cgccttaaaa
     4441 gcaagcggaa aattactcat cctcgcccag gtcatgccga tttggttggg gggattaagt
     4501 accgttttga tgatttgcga aattctttgg agcgttcatc agctcgtgaa accaccatgc
     4561 gggtggcagt tggtgcagta gccaaacgtc tcttggctga gctggatatg gagattgcca
     4621 accatgtcgt ggtctttggt ggcaaggaaa tcgatgttcc tgaaaatctg acagtcgctg
     4681 aaattaagca aagagctgcc cagtctgaag tttctattgt caaccaagaa cgagaacagg
     4741 aaatcaagga ctatattgac caaatcaaac gtgatggtga taccatcggt ggggttgtgg
     4801 agacagtcgt cggaggcgtt ccagttggtc ttggttccta tgtccaatgg gatagaaaat
     4861 tggatgcaag attggctcaa gctgttgtct ctatcaatgc ctttaaaggg gtggaatttg
     4921 gtcttggctt tgaggctggt tatcgtaaag gcagccaagt tatggatgaa attctctggt
     4981 ctaaagaaga cggttatact cgccgtacca ataatctagg tggttttgaa ggtggtatga
     5041 ctaatgggca acccatcgtt gttcgtgggg ttatgaaacc cattcctact ctttataaac
     5101 ctcttatgag tgtggatatc gaaacccacg aaccttacaa ggcaaccgtg gagagaagtg
     5161 atccgactgc tcttccagct gcaggaatgg tcatggaagc agttgtagca acggttctgg
     5221 cgcaagaaat cctcgaaaaa ttctcatcag ataatcttga ggaactaaaa gaagcggtag
     5281 ccaaacaccg agactataca aagaactatt aaggagttcc tatggcaaaa acaatctata
     5341 tcgcaggtct tgggttgatt ggagcctcta tggcacttgg tatcaaacgc gatcatccag
     5401 attatgaaat tttaggttat aatcgtagtc aagcttcgag agacatcgcc ttgaaagaag
     5461 gcatgattga ccgtgcaacg gatgattttg ctagttttgc tcctttggca gatgtcatta
     5521 tcctcagctt gccaatcaaa caaactattg ctttcattaa ggagttggcc aatttggatt
     5581 tgcgagaagg cgttattatt tcagatgctg gttcgaccaa gtcaaccatt gtggatgcgg
     5641 cggagcagta tttggctggc aagtctgttc gctttgtcgg ggcccatccc atggctggta
     5701 gtcacaagac aggggctgct tcggcagatg tcaatctttt tgaaaatgcc tattatatct
     5761 ttacaccttc aagcctgaca agtcaggaca cgcttaagga aatgaaggat ctgctttcag
     5821 gtcttcatgc tcgttttatc gagattgatg ccaaggagca tgatcgtgtc acttctcaga
     5881 ttagccattt tcctcatatt ttggcttcta gtctcatgga gcagactgcg gtctatgctc
     5941 aagagcatga gatggcaagg cgctttgcgg caggtggttt tcgagatatg acccgaattg
     6001 cggaaagcga gccaggaatg tggacctcca ttctcttgtc caatagcgag accattctgg
     6061 atagaattga ggatttcaag gaacgtttgg aagcgattgg tcaggccatt agtaagggag
     6121 atgaagagca aatttggaac ttttttaacc aagcgcgtga acaacgccag accatggaaa
     6181 tccataagcg tggtggtgtg gatagctctt acgacctcta tgttgacgtt cctgatgaag
     6241 aagatgtcat cctgcggatt ttggaactgc tacgtgggac ttccttggtt aacatccaca
     6301 tcaacgagga aaatcgtgag gatattcacg gaatcctaca aatttcattt aaaaatgctc
     6361 aagacttgga aagagccgag catctcataa caaaaaatac cgactataca gtcgtcatta
     6421 aataaggaga agaacatgtc aaatatttac gatagtgcaa acgaactcag tcgcggtcta
     6481 cgcggattac cagaatacaa ggctgttaaa gcagctaaag atgcgattgc agcagatgct
     6541 gaggcaagca aaatctttac agaatatctt gcctttcaag aggaaattca aaaactagca
     6601 cagacaggtc aaatgccaga tgcgtccttc caagctaaga tggaaggctt tggtaaacag
     6661 attcaaggaa atagcctctt gtcagaattc tttaccaagc aacaacaatt ggccatttac
     6721 ctttctgaca ttgaaaaaat tgttttcgaa ccagtttcag aattgctgaa ataagaaaag
     6781 aactatccta aagtcaggaa tttttaagga atttctggct ttttatgata aaataggtaa
     6841 aagtattgca agtatgaggt ccagtatgaa actaaaaaca aacattcgcc atttacatgg
     6901 tagtatccgc gtcccaggtg acaagtctat cagccaccgt tccattatct ttggaagttt
     6961 ggctgagggt gagaccaagg tttatgatat tctgcgaggt gaagacgttc tttcgaccat
     7021 gcaggttttt cgtgaccttg gtgttgaaat tgaggataaa gatggggtta ttaccgttca
     7081 aggtgtaggc atggctggct taaaagcgcc gcaaaatgcc cttaatatgg gaaattctgg
     7141 cacctcgatt cgcctgattt caggtgtcct tgctggtgca gatttcgaag tagagatgtt
     7201 tggagatgat agtctttcca aacgtcctat ggaccgtgtg acccttccac tgaaaaaaat
     7261 gggcgtcagc atctcagggc aaactgaacg agacttgcct ccccttcgct taaaagggac
     7321 gaaaaaccta agacctattc attatgagtt gccaattgcc tctgcccaag tcaagtcagc
     7381 cttgatgttt gcagccttac aagctaaggg ggagtcagtt attatcgaaa aagagtacac
     7441 ccgtaatcat actgaagata tgttgcaaca atttggtggt catttaagtg tggatggtaa
     7501 gaaaatcaca gtccaagggc cacaaaaatt gacaggacag aaggtggtcg taccaggaga
     7561 tatttccagt gcagcctttt ggttagtcgc aggtttgatt gctccaaatt ctcgtctagt
     7621 gctgcagaat gtggggataa acgaaactcg caccggtatt attgatgtca ttcgtgccat
     7681 gggtggaaaa ttggaaataa ctgaaatcga tccagtcgct aaatctgcaa ccttgattgt
     7741 tgagtcttct gacttgaaag gaacagagat ttgtggcgct ttgattccac gtttgattga
     7801 tgaattgcct attattgccc tacttgcgac ccaagcccaa ggtgtaacag ttatcaagga
     7861 tgctgaggag ctcaaggtca aggaaacaga ccgtattcag gttgtggcag acgccttaaa
     7921 tagtatggga gcagatatta ctcctacggc agatgggatg attatcaaag gaaaatcagc
     7981 tcttcacggt gctagagtca atacgtttgg tgaccaccgt atcggcatga tgacagctat
     8041 cgcagcccta ttggttgcag atggagaggt ggagcttgac cgtgcagaag ccatcaatac
     8101 cagctatcct agtttctttg atgatttgga gagcttgatt catggctaag gtgttattag
     8161 ggtttatggg ggctggaaaa tcgactattg caagaggctt ggaccctaat taccttgata
     8221 tggatgctct gattgagaag cgcctaggta tgtccattgc gaattttttc gctgaaaagg
     8281 gagaagcagc ctttcgtcag gtagaatcag aagtcctagc tgatttatta caaagagacc
     8341 aagtcgtgtc aactggagga ggagtggtta tttctcagag aaatcgtgac ttactcaaga
     8401 ctaatacaga taacatctac ctgaaagcag attttgaaac cctctaccta cgtatcgcag
     8461 ctgataagga caatcagcga ccgctttttc taaataatag caaggaagaa ctagtagcta
     8521 tttttcaaga aagacaggct tggtatgagg aagtggctag tcgggttttg gatgtgacca
     8581 agctaagccc agaggaaatt atagaggaac taagatgaaa attgcttatc taggtcccaa
     8641 aggatcattt tcacaccacg ttgtgcagac agcttttcct catgaggaat tgcaggcttt
     8701 tgccaacatt acagatgtca tcaaggctta tgagcaagga ttagtggact attctgtggt
     8761 gccagttgaa aattctattg agggtagtgt tcatgaaacc ttggactatc tttttcatca
     8821 ggctcacatc caagcagtag cagaaatcgt tcagcctatt catcagcagt tgatggtggt
     8881 tccaggccat actaagattg aaaagatttt ttcacatcca caggccttgg ctcaaggaaa
     8941 gaaattcatc gatgaacaat atccagaggc tcaaatcgag gtaacagcta gtacagctta
     9001 tgcggcccgt tttatttccg aacatccaga tcagcccttt gcagcaattg cacctagaag
     9061 ttctgctgaa gaatatggat tggaactgat tgccgaggat attcaggaaa tggaagccaa
     9121 tttcacacgt ttctggcttc taggagctga aaagcctagt attcccttgc aagcacaaac
     9181 tgaaaagatg agtttggcct tgacattacc tgacaacctt ccaggtgcac tttataaggc
     9241 cctgtcgacc tttgcttggc gaggaattga cttgacaaaa attgaaagtc gtccactcaa
     9301 gacagcactg ggtgaatact ttttcattat cgatgtggat tataccgata aggacttggt
     9361 ccactttgcc caaaaagaat tagaagcgat tggaatccag tataaaattc tgggtgccta
     9421 tcctatttat ccaatatcag accatggaaa ggagagaaga tgagtaaaga aaatccctta
     9481 agtcatcatg agcagttgcg ttatgattat ttgctaaaaa atattcacta tctcaatgag
     9541 agagaaaaaa atgagtttgt ctatttgcaa gaaaagctaa ctcttgctag gggaaatagt
     9601 agctctagct tggaacaaga aagagaagag caggttgact taccaagcta tgcgaaccgg
     9661 agtcgctcac aatccaaatc acaagcactc tctttccctc caaaaaagaa aagacggaag
     9721 ctccgtctta aacgaatttt tatggtgatt ttttcacttt tggtctgtgt ggctttggcc
     9781 atggtattca tgtttttgcg tggttaccaa gatgctagcg caaagaaaac tgctgatgcc
     9841 cgggcagctc aagtagaagt ctttaatggt caggacacta gagatggagt taatatttta
     9901 atcatgggta ctgatggtcg aatcggccag aacagtgttg agacacgaac tgactctatt
     9961 atggtattaa atgtcggggg ctcagataag aaaatgaagc tagtcagttt catgcgtgat
    10021 aatttggtct atatagatgg ttatagtcaa gtgattaacg gtagaaaaca gacagataac
    10081 aagttaaacg tagcctacga gttaggagaa caagaagggc aaaaaggggc agaaatggtt
    10141 cgccaagtct tgaaagataa ttttgacttg gacattaagt actacgcctt ggttgatttc
    10201 caggcctttg ccacagcgat tgacacgctt ttccctgatg gggtgacgat tgatgctcaa
    10261 ttttcaacat tgaatgggcg tcccctaaca gaagctacag tcggagatga tttatacgct
    10321 accgagactg agtctccaac ccaaaccatt aaagtcggaa aacagcagat gaatggctcg
    10381 accctgctca attatgctcg tttccgtgat gatgatgagg cggattacgg ccgtacaaaa
    10441 agacagcaac aagttttaac agcaatttta gagcaaatta aagatcccac taaacttttc
    10501 actggttctg aagctcttgg aaaggttttt gctatgacct caaccaacgt accctatact
    10561 ttcctcctaa caaacggttt atctgttttg gatggagcaa aaaatggtat tgaaaaattg
    10621 acgattccag aactgggtga ctgggtagat gcctatgatg tttatggagg cttgggcttg
    10681 ctggttgatc aaaacaaata tcaaaccaag ctcgctcaaa tgggcttgag ataagataat
    10741 actcaatgaa aatcaaagtg caaattagga agctagccgc aggctgctaa aaacacagtt
    10801 ttgaggttgt agataagact gacgaagtca gttacctata cctacggcaa ggtgaagctg
    10861 acgtggtttg aagagatttt cgaagagtat aagacagaaa aatcaaagtt tgatagctat
    10921 ttggatagca gtcaggcttt gattttttac agtctgcaat agattttcac cccttgtttg
    10981 tggtataatg aaacgatacg atagaaagaa taatgaacaa tatgactgat ttaaaagcaa
    11041 ttcaggctcg tagtctggag atggctgaat attttgtggc cttttgtaaa gaacatgatt
    11101 tgctctgtta tctctgtggc ggaggcgcta ttggtaccct tcgaaacaag ggatttattc
    11161 cttgggacga cgacctagac ttttttatgc ctcgtaaaga ttatgagaaa ttagcagaat
    11221 tatggcctcg ttatgcagat gaacgttatt tcttgtcaaa gagtcacaag gattttgttg
    11281 atcgtaatct ttttattacc attcgtgaca agaaaaccac ctgtatcaag ccttatcagc
    11341 aggatttgga tttgccacat ggtctggcct tggatgtttt gcctttggat tattatccga
    11401 aaaatccagc tgagcggaaa aaacaggttc gttgggcctt gatttattca ctcttttgtg
    11461 cgcaaactat tccagaaaag catggtgctc tcatgaaatg gggaagtcgc attttactgg
    11521 gtttgactcc aaaatctctc cgttatcgca tctggaaaaa agctgagaaa gaaatgacta
    11581 agtatgattt ggctgattgt gatggcatta cagaattatg ctcaggtcct ggctacatga
    11641 gaaacaagta cccaatcaca tcttttgaag acaatctttt ctttccattt gaaggaacag
    11701 agatgcctat tccaatcggc tatgatgtct atctcagaac tgcttttggg gattatatga
    11761 cgcctccacc agcagacaag caggtaccgc atcatgatgc tgtcatcgct gatatggata
    11821 agtcttatac agaatacaag ggagaatatg gtggctaaga aaaaaatctt attttttatg
    11881 tggtcttttt ctcttggagg tggtgcagag aagattctat caaccattgt ttcaaatctg
    11941 gatccagaaa agtatgatat tgatattctt gaaatggagc actttgacaa gggatatgaa
    12001 tctgttccaa agcatgtacg cattttaaaa tcccttcaag attatcgcca aaccagatgg
    12061 ttacgagctt ttttgtggag aatgagaatt tattttccaa gactggctcg tcgtttgctt
    12121 gtaaaagatg attatgatgt tgaagtttct tttaccatta tgaatccacc actgttgttc
    12181 tctaaaagaa gagaagtcaa gaagatatct tggattcatg gaagtattga agaacttctt
    12241 aaggatagct ctaaaagaga atcacataga agccagttgg atgctgcgaa tacaattgta
    12301 gggatttcaa aaaagaccag caattctatc aaggaagttt atccagatta tgcttctaaa
    12361 ttacagacaa tctacaatgg atatgatttt cagactattc tagaaaaatc tcaagagaag
    12421 atcgatatcg agattgctcc tcaaagtatc tgtactatcg gacggatcga ggaaaataag
    12481 ggttctgacc gtgtagtgga agtgatacga ttattacacc aagagggaaa aaactatcat
    12541 ctctatttta tcggggctgg tgatatggaa gaggaactga aaaaacgagt caaagagtat
    12601 gggattgagg actatgtaca tttccttggt tatcaaaaaa atccttatca gtatttatct
    12661 cagacgaaag ttctcttgtc tatgtctaaa caagaaggtt ttcctggagt gtatgtggag
    12721 gccttgagtc tgggactccc ttttatctct acggacgttg gaggggctga ggaattatcc
    12781 caagaaggac gatttggaca aatcattgag agcaatcaag aggcagctca ggcgattact
    12841 aattacatga cttctgcctc aaactttgat gtcgatgagg ctagccaatt cattcaacaa
    12901 tttacaatta caaaacaaat cgaacaagta gaaaaactat tagaggagta gcatggaaac
    12961 tgcattaatt agtgtgattg tgccagtcta taatgtggcg cagtacctag aaaaatcgat
    13021 agcttccatt cagaagcaga cctatcaaaa tctggaaatt attcttgttg atgatggtgc
    13081 aacagatgaa agtggtcgct tgtgtgattc aatcgctgaa caagatgcca gggtgtcagt
    13141 gcttcataaa aagaacgaag gattgtcgca agcacgaaat gatgggatga agcaggctca
    13201 cggggattat ctgattttta ttgactcaga tgattatatc catccagaaa tgattcagag
    13261 cttatatgag caattagttc aagaagatgc ggatgtttcg agctgtggtg tcatgaatgt
    13321 ctatgctaat gatgaaagcc cacagtcagc caatcaggat gactattttg tctgtgattc
    13381 tcaaacattt ctaaaggaat acctcatagg tgaaaaaata cctgggacga tttgcaataa
    13441 gctaatcaag agacagattg caactgacct atcctttcct aaggggttga tttacgaaga
    13501 tgcctattac cattttgatt taatcaagtt ggccaagaag tatgtggtta atactaaacc
    13561 ctattattac tatttccata gaggggatag tattacgacc aaaccctatg cagagaagga
    13621 tttagcctat attgatatct accaaaagtt ttataatgaa gttgtgaaaa actatcctga
    13681 cttgaaagag gtcgcttttt tcagattggc ctatgcccac ttctttattc tggataagat
    13741 gttgctagat gatcagtata aacagtttga agcctattct cagattcatc gttttttaaa
    13801 aggccatgcc tttgctattt ctaggaatcc aattttccgt aaggggagaa gaattagtgc
    13861 tttggctcta ttcataaata tttccttata tcgattctta ttactgaaaa atattgaaaa
    13921 atctaaaaaa ttacattaga acgggggtga ccaagtgatt gatgggaaac gattattatt
    13981 tagtttgacc atagtcagtt atgccttgac gctagtaagt ggaattgtgt atctgtttaa
    14041 taataataat gttagcttac tttctacttt attgttcttg ttggttagta gcttaattgc
    14101 ttgttggaat gatatcaagt attacttaat ccattttatt ttctatttaa ccatttttgt
    14161 atttctggta tcaagaccga ccattgatta ttttagggat ggtgctttgg atacctatca
    14221 tccaatagcc tatcgttttg cctttatagt tgtcatggtt tcgattctgg gcttgaccac
    14281 aggaggcatt ctggctcgtt acttcatagc taggaagaaa ataaaagtag caaatatagg
    14341 aaattctcta aaagaggttt atatcaagcg gttacgcttt gtatcactag gagtttttct
    14401 tctaacctat cctttctatt tcattcggtt atttgaacgg ctcttgtatc gtttgcagac
    14461 ttcctactat gcctactatg caaattttga aagtaaactg ccttatttta cctacatttt
    14521 gtctaccttt acggtctatg caatgtgtat gtatctggca accaagccaa agaaattgca
    14581 ggccacagca gtgcttgtct cctttattgc agctaatact attcatttgg caattgggac
    14641 acgaaatccc tttattttaa gtattttatt tgcttttgtt tattacttta tgcgggagca
    14701 aactgaaaaa ggaaaatgga ttgggtttaa agaaaagtta gcgatttttg taggttctcc
    14761 tattctcatg ttagcgatgg gagtactcaa ttatgtacgg gataatgtcc aagtttccca
    14821 tacaggtttc tgggatatct tacttgactt tatctataaa caagggacta gttttggtgt
    14881 tctggctcga ggttttctat ttaacagtag cctcccttac cgagatttcc gtaattttac
    14941 ttttggtcct gttcttgatt attttgcaag ggggagtttg ggagccattt tcggaggaaa
    15001 agcctttgaa catacaacca atagtgtgga actagctatt gatagtaata gttatgccca
    15061 caatctatcc tatcttgtct tgaacaagga atacctgaaa gggcatggta tcggaagtag
    15121 ttatatcatg gagttgtata ccgactatgg tatgattgga gtctttctgc ttagtttctt
    15181 actcggcgta ttatttatag ccatgctgca agtagcctat cgctcaagga caatcctatt
    15241 tgctttatcc ctactcatct tgaataatct attctttatg ccaagaagca gcttttcaga
    15301 aagtttcttc aatttattta caatgcaatt ctggggaatt gttcttgtga ttatatttgt
    15361 agcaaaaatg cttacaaagg aaaactagta tctactaaac aaaggagaaa aaaatcatgt
    15421 ttgaacatta ttcagtagct gatttgtttg caaatcttta taaaaaacga aaagcaaata
    15481 ttttagctct catcgcttta tttgctctca ttgctgtacc atttacaatt aaagcagtta
    15541 ggaataaaaa cactgtcaaa gatacaacaa gttattcaac ttatcttatc tataaaatca
    15601 ctcctccaga agattcggcc aaaacgattt tgaatcatca aattggtggt tatagtgatt
    15661 tttatgggaa attgattgat ggtaatttga atggagctta tcttttcaat gatgtagaac
    15721 ccagtgagtt gaaaaaaatt gccagtgaat tagacacgac ggaaacaacc ttgaaaaatt
    15781 caacgaatga ctattggtgg aaaaaattga ccgtctacta tatgattgac gatgcagggg
    15841 ttggtgtgaa aattttgaca tcaagtaaag atgccaatga cttgttagag aaaaaaattg
    15901 atgggttgat tgagaaattt aaacatgctt atgcaaatgt gaaaattgaa aaactggaaa
    15961 ccatcaactc taaagaattg aacgcaaatg gtgaaacagc gcttggctta aatgtgaaaa
    16021 atctgattct tcgtttagtt gttattggag tggtttgtgt gattttggtt gtgatgggaa
    16081 atgtgttagt ttatctcttt aatccaacaa tcaatagagt aggtgatttt tctcagtatc
    16141 aaattgattt tgtaacagag attacaacaa ttgctaacct agcagatgtt ttgtcataca
    16201 aaaatactgg acaggaattg accatcgtta gctcaaataa agctatccta gataaattga
    16261 aacagagtca agaagcttta aaaggaatgc attttgtaga tttgcaggat gtatcatctc
    16321 ttttggaaag agatacagtc cttcttgttg aagagtacgg agtgactcgt tataagaaat
    16381 ttgagcaaag tcttcaaatt ctcagaaact taaatcgttc tatcctcggt gtagcaacct
    16441 ttaaattata agtattctga tgtatgaggt cttcaaaatc ggctaagagc tgattttgaa
    16501 ggctttttct atctgtctgc agaaattttc ttttggagtc tcctatagaa agaccctagg
    16561 gaaaatctat ccgtatagag gagccttctt ttgataaaat cgtaaaaatc tagtataata
    16621 gatagaactg aaagtatgag gttactagca atgaaaatga aacaaattag tgatacaact
    16681 ttaaaaatca ctatgtcttt agaggatttg atggatcgtg gaatggagat tgctgacttt
    16741 ctcgttcctc aagaaaaaac agaagagttc ttttatgcta tcttggatga gctagagatg
    16801 cctgatagct ttctggatac aggcatgttg agcttccgtg tgactccaaa acctgataag
    16861 gtagatgtct ttgtgaccaa gtcaaagatt gaccaaaatc tagattttga agacttatcg
    16921 gatttgccag atatggaaga attggctcaa atgtctccag atgaatttat caaaaccctg
    16981 gaaaaaagca tcgcagacaa aaccaaggat gatatcgaag ccattcaatc tcttgagcaa
    17041 gttgaagcca aggaagaaga gcaagagcag gctgaacaag aagctgagag taagaaagaa
    17101 ccttacatct actacatcct ttcttttgct aagttggctg acttggtagt ttttgccaag
    17161 acagtgactt ttgaaatgga aacttctgaa ctctacaaaa tgaacgagcg ctattatttg
    17221 accattttag tggatattga aaatcatcca agcccatatc cagcttggct gttggcccgt
    17281 atgcgcgagt ttgcagacga tagtgatatc agtcgctcag tcttacaaga gtatggtcaa
    17341 gtcttgatga gtcacgatgc agtgctcaat ctgcaaaaaa tcggctaatc ctttctggaa
    17401 agctattttc agattttaag aagagcgaat cgtctgattc gttttttctt tttatactga
    17461 aatagtgatt tactataata ggaattttca caaaattctg ttataatggc tatattagaa
    17521 aatttcgagg agataaagat gacagttaaa attgctttac taggatttgg taccgttgca
    17581 agtggtgtgc ctttcctcct aaaggaaaat ggaggaaaaa tcaatcaatc agcacattca
    17641 gatatcaaag ttgctaaggt attggtcaag gatgaagatg aaaaaaatcg cttgcttgca
    17701 gcagggaatg actttaactt tgtaaccaat gtggatgata ttttatcaga ccaggatatt
    17761 actatcgtag tggaattgat ggggcgtatt gagcctgcta aaacctttat cactcgtgcc
    17821 ttggaagctg gaaaacacgt tgttactgct aacaaggacc ttttagctgt ccatggcgca
    17881 gaattgctag aaatcgctca agctaacaag gtagcacttt actacgaagc agcagttgct
    17941 ggtgggattc caattcttcg tactttagca aattccttgg cttctgataa aattacgcgc
    18001 gtgcttggag tagtcaacgg aacttccaac ttcatggtga ccaagatggt ggaagaaggc
    18061 tggtcttacg atgatgctct tgcggaagca caacgtctag gatttgcaga aagcgatccg
    18121 acgaatgacg tagatgggat tgatgcagcc tacaagatgg ttattttgag ccaatttgcc
    18181 tttggcatga agattgcctt tgatgatgta gcccacaagg gaatccgcaa tatcacacca
    18241 gaagacgtag ctgtagctca agagtttggt tacgtagtga aattggttgg ttctattgag
    18301 gaaacttctt caggtattgc tgcagaagtg actccaacct tcctacctaa agcgcaccca
    18361 cttgctagtg tgaatggcgt aatgaacgct gtctttgtag aatctatcgg tattggtgag
    18421 tctatgtact acggaccagg tgcgggtcaa aaaccaactg caacaagtgt tgtagctgat
    18481 attgtccgta tcgttcgtcg tttgaatgat ggtaccattg gcaaagactt caacgaatat
    18541 agccgtgact tggtcttggc aaatcctgaa gatgtcaaag caaactacta tttctcaatc
    18601 ttggctccag actcaaaagg tcaggtcttg aagttggctg aaatcttcaa tgctcaagat
    18661 atttccttta agcaaatcct tcaagatggc aaagagggtg acaaggcgcg tgtcgttatc
    18721 atcacacaca agattaataa agcccagctt gaaaatgtct cagctgaatt gaagaaggtt
    18781 tcagaattcg acctcttgaa taccttcaag gtgctaggag aataagatga agattattgt
    18841 acctgcaacc agtgccaata tcgggccagg ttttgactcg gtcggtgtag ctgtaaccaa
    18901 gtatcttcaa attgaggtct gcgaagaacg agatgagtgg ctgattgaac accagattgg
    18961 caaatggatt ccacatgatg agcgtaatct cttgctcaaa atcgctttac aaattgtacc
    19021 agacttgcaa ccaagacgct tgaaaatgac cagtgatgtc cctttggcgc gcggtttggg
    19081 ttcttccagc tcggttattg ttgctgggat tgaactagcc aaccaactgg gtcaactcaa
    19141 cttatcagac catgaaaaat tgcagttagc gaccaagatt gaagggcatc ctgacaatgt
    19201 ggctccagcc atttatggta atctcgttat tgcaagttct gttgaagggc aagtctctgc
    19261 tatcgtagca gactttccag agtgtgattt tctagcttac attccaaact atgaattacg
    19321 tactcgcgac agccgtagtg tcttgcctaa aaaattatct tataaggaag ctgttgctgc
    19381 aagttctatc gccaatgtag cggttgctgc cttgttggca ggagacatgg tgaccgctgg
    19441 gcaagcaatc gagggagacc tcttccatga gcgctatcgt caggacttgg taagagaatt
    19501 tgcgatgatt aagcaagtga ccaaagaaaa tggggcctat gcaacctacc tttctggtgc
    19561 tgggccgaca gttatggttc tggcttctca tgacaagatg ccaacaatta aggcagaatt
    19621 ggaaaagcaa cctttcaaag gaaaactgca tgacttgaga gttgataccc aaggtgtccg
    19681 tgtagaagca aaataaagaa tagaagatag gatggggaaa ctcttaacca gagggtttca
    19741 tatccttttt gtgaaaagaa gtttatactc aatgaaaatc aaagagcaaa ctaggaagct
    19801 agccgcaggc tgctcaaaac agtgttttga ggttgcagat agaactgacg acgtcagtaa
    19861 catatatacg gtaaggtgac gctaacgtgg tttgaagaga ttttcgaaga gtattagtta
    19921 aaaacgtgat aaaggagaaa taaagatggc agaaatttat ctagcaggtg gttgtttttg
    19981 gggcctagag gaatattttt cacgcatttc tggagtgcta gaaaccagtg ttggctacgc
    20041 taatggtcaa gtcgaaacga ccaattacca gttgatcaag gaaacagacc atgcagaaac
    20101 ggtccaagtg atttacgatg agaaggaagt gtcactcaga gagattttac tttattattt
    20161 ccgagttatc gatcctctat ctatcaatca acaagggaat gaccgtggcc gccaatatcg
    20221 aactgggatt tattatcagg atgaagcaga tttgccagct atctacacag tggtgcagga
    20281 gcaggaacgc atgctgggtc gaaagattgc agtagaagtg gagcaattac gccactacat
    20341 tctggctgaa gactaccacc aagactatct caggaagaat ccttcaggtt actgtcatat
    20401 cgatgtgacc gatgctgata agccattgat tgatgcagca aactatgaaa agcctagtca
    20461 agaggtgttg aaggccagtc tatctgaaga gtcttatcgt gtcacccaag aagctgctac
    20521 agaggctcca tttaccaatg cctatgacca aacctttgaa gaggggattt atgtagatat
    20581 tacgacaggt gagccactct tttttgccaa ggataagttt gcttcaggtt gtggttggcc
    20641 aagttttagc cgtccgattt ccaaagagtt gattcattat tacaaggatc tgagccatgg
    20701 aatggagcga attgaagttc gttctcgttc aggcagtgct cacttgggtc atgttttcac
    20761 agatggaccg cgggagttag gcggcctccg ttactgtatc aattctgctt ctttacgctt
    20821 tgtggccaag gatgagatgg aaaaagcagg atatggctat ctattgcctt acttaaacaa
    20881 ataaaacaga gagtggggct tcccactttc ttcatttcta gaatatgaat agaagggatt
    20941 tatgaaacac ctattatctt acttcaaacc ctacatcaag gaatcaattt tagccccctt
    21001 gttcaagctg ttagaagctg tttttgagct cttggttccc atggtgattg ctgggattgt
    21061 tgaccaatct ttacctcagg gagatcaagg tcatctctgg atgcagattg gtctgctcct
    21121 tatctttgca gtaattggcg ttttagtggc cttgatagct caattttact cagcaaaggc
    21181 agcagtaggt tttgctaagg aattgacaaa cgatctttat cgtcatattc tttccttgcc
    21241 caaggacagc agagaccgtc tgacaacttc tagtttggtc actcgcttga cttcggatac
    21301 ctaccagatt cagactggta tcaatcaatt cctgcgtctc tttttacgag cgcccattat
    21361 cgtttttggt gccattttta tggcttatcg aatctcagct gagttgactt tctggttctt
    21421 agtcatggtt gccattttga ccattgtcat tgtagggtta tctcgattgg tcaatcctct
    21481 ctacagtagt ctcagaaaga aaacggacca actggttcag gaaacgcgcc agcaattgca
    21541 agggacgcgg gttattcgtg cttttggtca agaaaaacga gagttacaga tttttcaaac
    21601 ccttaaccaa gtttatgcta gattacaaga aaagacaggt ttctggtcta gtttattaac
    21661 acctctgacc tatctgattg tcaatggaac tcttctcgtt attatctggc aaggctatat
    21721 ttcaattcaa ggaggagtgc tcagtcaagg tgctctcatt gctcttatca attacctctt
    21781 acagattttg gtggaattgg tcaagcttgc catgctgatc acttccctca accagtccta
    21841 catctcagcc aagagaatcg aggaggtctt tgcggaatct cccgaaaaca tccattcaga
    21901 attagaacaa aagcaagtta ccagtgatcg ggttttacaa gtccaagaat tgacctttac
    21961 ctatcctgat gcggcccagc cttctctgag agacatttcc tttgatatga ctcaaggaca
    22021 aatccttggt atcattgggg ggactggttc tggtaaatca agcttggtgc aactcttact
    22081 tggactttat ccagtagaca aggggaacat tgacctttat caaaatggac gtagtcctct
    22141 taatttggag cagtggcggt cttggattgc ctatgtacct caaaaggtcg aactctttaa
    22201 aggaaccatt cgttccaact tgactctagg tttcaatcaa gaagtatctg accaggaact
    22261 ctggcaggcc ttggagattg cgcaagctaa ggattttgtc agtgaaaagg aaggactctt
    22321 ggatgctcta gttgaggcag gggggcgaaa tttctcaggt ggacaaaaac aaagattgtc
    22381 tatcgcccga gcagtcttgc gccaagctcc gtttctcatc ctagatgatg caacctcggc
    22441 actggatacc attacagagt ccaagctctt gaaagctatt agagaaaatt ttccaaacac
    22501 gagcttaatt ttgatctctc aacgaacctc aactttacag atggcggacc agattctcct
    22561 cttggaaaaa ggtgagttgc tagctgttgg caagcacgat gacttgatga aatccagcca
    22621 agtctattgt gaaatcaatg catcccaaca tggaaaggag gactagaatg aaacgacaaa
    22681 ctgtaaacca gacgctcaaa cgtttagtcg tagatttagc aagccatcct ttcctccttt
    22741 tcctagcctt tctaggaact attgctcaag ttggcttatc aatttaccta cctattctga
    22801 ttgggcaggt cattgaccaa gtcctagtgg ctggttcatc accagttttt tggcagattt
    22861 ttctccagat gctcttggtg gtaataggaa atactctggt acaatgggcc aatcctctcc
    22921 tctataatcg tctaatcttc tcttatacca gagatttacg ggagcgaatc atccataagc
    22981 tccatcgttt accgattgcc tttgtagata ggcaaggtag tggagagatg gttagtcgtg
    23041 taaccacgga catcgaacag ttggcagctg gcttgaccat gatttttaac caatttttca
    23101 ttggtgtttt gatgattttg gtcagtattc tagccatgct ccaaattcat ctcctcatga
    23161 ctctcttagt cttgctgttg acgccactgt ccatggtgat ttcacgcttt attgccaaga
    23221 aatcctatca tctcttccag aagcaaacag agacgagggg aattcagact cagttgattg
    23281 aagaatcgct tagtcagcag actataatcc agtccttcaa tgctcaaaca gaatttatcc
    23341 aaagattgcg tgaggctcat gacaactact caggctattc tcagtcagcc atcttttatt
    23401 cttcaacggt caatccttcg actcgctttg taaatgcact catttatgcc cttttagctg
    23461 gagtaggagc ttatcgtatc atgatgggtt cagccttgac cgtcggtcgt ttagtgactt
    23521 ttttgaacta tgttcagcaa tacaccaagc cctttaacga tatttcttca gtgctagctg
    23581 agttgcaaag tgctctggct tgcgtagagc gtatctatgg agtcttagat agccctgaag
    23641 tggctgaaac aggtaaggaa gtcttgacca gtgaccaagt taagggagct atttccttta
    23701 aacatgtctc ttttggctac catcctgaaa aaattttgat taaggacttg tctatcgata
    23761 ttccagctgg tagtaagata gccatcgttg gtccgacagg tgctggaaaa tcaactctta
    23821 tcaatctcct tatgcgtttt tatcccatta gctcgggaga tatcttgctg gatgggcaat
    23881 ccatttatga ttatacacga gcatcattga gacagcagtt tggcatggtg cttcaagaaa
    23941 cctggctcac acaagggacc attcatgata atattgcctt tggcaatcct gaagccagtc
    24001 gagagcaagt aattgctgct gccaaagcag ctaatgcaga ctttttcatc caacagtttc
    24061 cacagggata cgataccaag ttggaaaatg ctggagaatc tctctctgtc ggccaagctc
    24121 agctcttgac catagcccga gtctttctgg ctattccaaa gattcttatc ttagacgagg
    24181 caacttcttc cattgataca cggacagaag tgctggtaca ggatgccttt gcaaaactca
    24241 tgaagggccg cacaagcttc atcattgctc accgtttgtc aaccattcag gatgcggatt
    24301 taattcttgt cttagtagat ggtgatattg ttgaatatgg taaccatcaa gaactcatgg
    24361 atagaaaggg taagtattac caaatgcaaa aagctgcggc ttttagttct gaataagcca
    24421 ttctcttttg aaagtttatg gacgaaaaaa gttgccttcg agtgactttt ttgttacaat
    24481 agctagaaaa attgttcact gtaatactca atgaaaatca aagagcaaac taggaagcta
    24541 gccgtaggtt gctcaaagca cagctttgag gttgtagata agactgacga agtcagctca
    24601 aaacactgtt ttgaggttgc agatagaact gacgaagtca gctcaaaaca ctgttttgag
    24661 gttgcagata gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact
    24721 gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga agagagtaca
    24781 acttgtcttt taaaaaagga gtctataatg aaagtctttc agcatgtaaa tatcgtgact
    24841 tgtgatcaaa atttccatgt ttatcttgat ggaatcttag cagtcaagga ttctcaaatc
    24901 gtctatgttg gtcaagataa gccagcattt ttagaacaag ctgagcagat tatagactat
    24961 cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc aatgacaggt
    25021 ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta tatctggcca
    25081 gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc cctgacagag
    25141 atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg tgtggatatc
    25201 cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc tccgactctc
    25261 ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc catcatagac
    25321 gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca ttctccgtat
    25381 agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct aaatattccg
    25441 ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa acgatacggc
    25501 aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc cgtatttgct
    25561 cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca agtggctatc
    25621 gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat tatccagctc
    25681 caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa taacaatcta
    25741 gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag tggggatgcc
    25801 agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc taaggccctt
    25861 ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt tctggtcatt
    25921 caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca cctggtttat
    25981 gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt tgttaagcaa
    26041 ggtcaagtcc tgacagtaga actttaaaag aaaaatca
//
LOCUS       DAVKWS010000024        24678 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKWS010000024 DAVKWS010000000
VERSION     DAVKWS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.024"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            196..477
                     /locus_tag="VW852_001110"
     CDS             196..477
                     /locus_tag="VW852_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV0272268.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            609..1553
                     /gene="manA"
                     /locus_tag="VW852_001111"
     CDS             609..1553
                     /gene="manA"
                     /locus_tag="VW852_001111"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV0272269.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1574..3052)
                     /locus_tag="VW852_001112"
     CDS             complement(1574..3052)
                     /locus_tag="VW852_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV0272270.1"
                     /translation="MIFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGPLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFRLDIEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKIFFDAMDFLASNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGLE"
     gene            3157..3906
                     /locus_tag="VW852_001113"
     CDS             3157..3906
                     /locus_tag="VW852_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV0272271.1"
                     /translation="MYHIKETAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKDYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(3926..4381)
                     /locus_tag="VW852_001114"
     CDS             complement(3926..4381)
                     /locus_tag="VW852_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0272272.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4401..5576)
                     /locus_tag="VW852_001115"
     CDS             complement(4401..5576)
                     /locus_tag="VW852_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0272273.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5635..6480)
                     /locus_tag="VW852_001116"
     CDS             complement(5635..6480)
                     /locus_tag="VW852_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0272274.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6605..7162
                     /locus_tag="VW852_001117"
     CDS             6605..7162
                     /locus_tag="VW852_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0272275.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFRETRKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7181..7648
                     /locus_tag="VW852_001118"
     CDS             7181..7648
                     /locus_tag="VW852_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV0272276.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7737..8366
                     /gene="upp"
                     /locus_tag="VW852_001119"
     CDS             7737..8366
                     /gene="upp"
                     /locus_tag="VW852_001119"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV0272277.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKVLQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8540..9130
                     /gene="clpP"
                     /locus_tag="VW852_001120"
     CDS             8540..9130
                     /gene="clpP"
                     /locus_tag="VW852_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV0272278.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9209..9457
                     /locus_tag="VW852_001121"
     CDS             9209..9457
                     /locus_tag="VW852_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV0272279.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9558..10718
                     /locus_tag="VW852_001122"
     CDS             9558..10718
                     /locus_tag="VW852_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0272280.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLLSPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10986..11855
                     /locus_tag="VW852_001123"
     CDS             10986..11855
                     /locus_tag="VW852_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0272281.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11859..12815
                     /locus_tag="VW852_001124"
     CDS             11859..12815
                     /locus_tag="VW852_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0272282.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSTSPTYGAFFGAMLVGALLSG
                     VVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12815..13579
                     /locus_tag="VW852_001125"
     CDS             12815..13579
                     /locus_tag="VW852_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272283.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13579..14289
                     /locus_tag="VW852_001126"
     CDS             13579..14289
                     /locus_tag="VW852_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272284.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNKGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14597..15253
                     /locus_tag="VW852_001127"
     CDS             14597..15253
                     /locus_tag="VW852_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0272285.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15361..16456
                     /gene="prfB"
                     /locus_tag="VW852_001128"
     CDS             join(15361..15432,15434..16456)
                     /gene="prfB"
                     /locus_tag="VW852_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV0272286.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16474..17166
                     /gene="ftsE"
                     /locus_tag="VW852_001129"
     CDS             16474..17166
                     /gene="ftsE"
                     /locus_tag="VW852_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV0272287.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKKRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17159..18085
                     /locus_tag="VW852_001130"
     CDS             17159..18085
                     /locus_tag="VW852_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272288.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANAPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18371..20551
                     /locus_tag="VW852_001131"
     CDS             18371..20551
                     /locus_tag="VW852_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0272289.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20603..21418
                     /locus_tag="VW852_001132"
     CDS             20603..21418
                     /locus_tag="VW852_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0272290.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKENTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21558..22901
                     /locus_tag="VW852_001133"
     CDS             21558..22901
                     /locus_tag="VW852_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0272291.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23278..24468
                     /locus_tag="VW852_001134"
     CDS             23278..24468
                     /locus_tag="VW852_001134"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV0272292.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         7519 a         4608 c         5380 g         7171 t
ORIGIN      
        1 tcctgatcga tttgccctgt tcttgtttcg ttttactata gaacgatttg aaggcgttta
       61 tgatatttag ctgtacgaga gtctttaaaa agtgttttga tggtttggat ttcttcttta
      121 gttgatttca tatcactatt atataatgct ttttgatttt agtatagtat tatcgtagaa
      181 aatggactcg gtgctatgga tacaccggat gagaatggtt atgtagcaga tgactatcgg
      241 attacttact tagaggccca catcaaggcc atgcgagatg ccatttacaa agacggggtt
      301 gacttgcttg gttatacgac atggggctgt atcgattcgg tttcagctgg cacaggggaa
      361 atgaataagc gctatggctt tatctatgta gatcgagata atgtaggaaa tgggactctc
      421 aaacgtagca agaagaagtc cttctattgg tacatgtcat ttatagcaat ggtgtaagca
      481 tcgagtagag cacatttgtt tactattttt ataaaatctt ctccctgctt tataagatgt
      541 aaaaaagagt tcaattcgaa ctcttttttt gctataatga ggggagaaaa atcagacagg
      601 agatcgacat gtcagaacca ttatttttac aatcagttat gcaagaaaaa atctggggtg
      661 gagccaagct acgtgatgag tttggctacg acatcccaag tgaaaaaatc ggagaatatt
      721 gggccatctc agcccatcca aatggagtct ctaaagttgc caatggtcgt tacgagggaa
      781 cagatcttgc tactttgtat gcggaacacc gtgaattatt tggcaatcgt ccagaacctg
      841 tatttccact tttgaccaag atcctcgatg ccaacgactg gctcagtgtc caagttcacc
      901 cagacgatgc ttatggactc gagcatgaag gcgaactcgg aaaaacagaa tgctggtaca
      961 ttatcgcagc ggatgaaggt tcagagatta tctacggtca caatgccaag tcaaaagaag
     1021 aactccgcca gcaaatcgag gacaagaact gggatgactt gttgaccaaa gtccctgtta
     1081 aggctggaga tttcttctat gtaccaagcg ggactatgca cgctatcggg gcgggtatct
     1141 taatccttga aacccagcag tctagcgata ccacctatcg cgtctatgac tttgaccgca
     1201 aggatgacaa aggcaacttg cgtgaacttc atcttgaaaa atccatcgat gtcttgaaca
     1261 ttggtgagcc agcaaatagc cgtcctgtaa ctgttaaagc agatgatttg cgttccactc
     1321 tccttgtatc taatgatttc ttcgcagttt acaagtggga aattactgga aaagttgact
     1381 ttgaaaagac agctgactac agcttattga gtgtcttagc tggtcaaggt cagctaactg
     1441 ttgacgggaa aaattatcca attcaaaaag ggagacactt tatcctacca agtgatgttg
     1501 aagcttggac tctggaaggg caaggtttgg aattgattgt tagccatcca taaaaagaaa
     1561 gggcctgagt tcactactca agtccttttt gattacataa attgggcaat gaagaccaca
     1621 atgatgatga ttggaatgac gaaacgaaga aacgaagaag gaagagccag acttggaaca
     1681 gtccttgttt ccatgctctt tcatcgagat ggagttcctc cattgcaaga gcctttttaa
     1741 agatatagcc tgtaaaaagt gaaaggtaga gagctccaaa tggcatgagg agattggaag
     1801 ccaagaagtc catagcgtca aagaagatct taccaaaaat gtgaacatcc gccatgacac
     1861 cgtaagatag ggctgaagga atgccaaaga caaaggtcaa aattcctaaa ataacactcc
     1921 atttggcacg tttgctgtta tcctggttgg tgatattgcc cacattgatc tccagcatca
     1981 cgacagaaga agtgacagtc gcaaagagga aaaggacgta gaaaatggtt ccaaaaggca
     2041 tcttgtcaaa gagttgaggc aagacgataa agagcaagct cggtccccct tcaatatcca
     2101 gtctgaaggg ggaccgagct tggaaaatgg ctagacctgc catgatggat atcgagatat
     2161 tcatggctac gatggagatt cctgactgga ctagattggt tttcttgtcc aagtaagaag
     2221 cataggtcag catgactgta acccctagtg agagggcaaa gaaagattgt cccagagcat
     2281 agaggggacc agcgctagtc agttttgaaa agtctggttt gaggaagtaa agaacccctt
     2341 ccatggcatt tggcaaactg agagagcgac cgatgataac aacaaagatg ataaagagca
     2401 ggggcatcat gactttcgaa gctctttcaa tccctttttg aaccccacgt gatacaataa
     2461 agatatttaa taagataaag gctgcttgag ctactagggc aatggctgga tttgaaatga
     2521 ttgaagtaaa taactgagca taatcacccg ttccaccaag ttggaacaat ttcccaaact
     2581 caatacctag atagactaga atccatcctc cgataacact gtaaaaagat aagaggataa
     2641 agagggcaaa ggcgccaatc caaccgataa agttgtactt gttattcttg cccagttttc
     2701 caaaggtttt gatagcggaa acaccagcac tacggccaag ggcaaactca gccagcagga
     2761 gagggaaacc gattaaaata gtggaaatga gaaagattag taaaaagcct ccaccgccat
     2821 tagcagcagt catgtaggga aacttccaaa cggaaccaag cccgatgacc gagccagcag
     2881 atgctagaat aaaaccaagt ttcgaccccc attgtgattt ttctgacata gttaaactct
     2941 gatctccttt ttcaaaataa gaaaaagcta cttgagttgt caaatagcct tctgtttgat
     3001 tgatagactt gactatccta tcatgttttc taaggtctgt caagaaaatc attttcaagt
     3061 tttcacacct ttctcaaaaa agttaaaaaa ttttctcaaa aacgcttgac tctgacctaa
     3121 ggggaggggt tatactatca ttgtaaggag gaaatcatgt accatataaa agaaactgcg
     3181 cagctttcag gtgtctctgt caagaccctg catcactatg acaagatagg actcttggtc
     3241 cccttaaagt cggaaaacgg ctatcgaacc tacagtcaag aggatttgga acgccttcag
     3301 gtcattcttt actacaaata tctaggcttt tctttagaga aaatagcaga gctgttaaag
     3361 gaagaaagga cagatttatt gccccatttg actaggcagt tggactatct aactcgcgaa
     3421 aggcaacatc tggatacctt gatttccacc ttgcaaaaaa ctattcaaga acaaaaagga
     3481 gaaagaaaaa tgaccattga ggaaaaattc acgggattta gctatcaaga caatcaaaaa
     3541 taccaccaag aagcggtaga gaaatatggt caagaagtca tgggacaagc gctcgaacgc
     3601 caaaaaggtc acgaagacga ggctacggcc gcctttaacc aagtctttca aactttggca
     3661 caaaatcttc aagttggttt acctgcaaca gcaaccgaaa accaagagca agcagccaag
     3721 ctcttgcaag ccattcgcac ttatggattt gactgctcta ttgaggtatt cggtcatatc
     3781 ggtaaagatt acgtctacaa cccagagttt aaggaaaaca ttgacaagtt tggttctgga
     3841 acagcccagt acacgtcaga tgccattgca gcttacgttc agacaaatgc agaaaaaata
     3901 ggctaggaat ttcctagcct attttttact tcaaatcata aagccagtcg tcaccgtttt
     3961 tgtagtaaaa gaattcactg agatcttctt ctagaaacac acgaagcata tcagacatat
     4021 catcggttgc aagttttaga tgagaaagat tttcaaagtc ctcccaccaa actttccctt
     4081 cgtctgaaga ctggagttca ccagtaaagt gttctgtctt gtaaaaaagg acgacataac
     4141 gataatcctt gtcgtcatac cagtttttga taccacagag ttggggtttg gaaatgatca
     4201 gaccagtttc ttctttcact tcacgaatga cagcatcgac aaaggattcg ccacgttcaa
     4261 catgaccacc aggaaaagta atgccaggcc agtcgggact aactcggtct tggaccagga
     4321 ccttatctcc gtttttaatc atacacatgt taacaaattc gactgcctct cttctgttca
     4381 ttcttcacaa cctttaatct ttaatcataa tgcagacttc ccgccaccca gccggtacag
     4441 agggcagaag tgatgttaaa gccacccgtg tgggcattga tatccataac ttcgcctgca
     4501 aagtggaggc caggtaccag cttactttca agggttttag gattgatttc cttgagactg
     4561 actccaccct tggtaacaaa ggactttgca agggacattt ttccagttac aggaatttta
     4621 agttctttaa tggactggac aagctgttct cgttcctttt cagtcagctg tttgactttt
     4681 tcaggatatc cttgtacaaa aaattcggcc aagcgttctg gtaacaaggt ttttaaagcg
     4741 tttttcaagg atttttcccg attttcttct agaaatgtaa ccaagtcctt ctcagaaagt
     4801 tgaggcaaaa catcgagtga gagaacctcc ccacctttga caaagctaga catgcgtagg
     4861 gcagcaggac ctgacaaacc aaagtgggta aagagtaaat catgagtgat gacatgctta
     4921 ccataactta gggtcacatc gtccaaagaa atcccttgta aggctttatg tggaaaatct
     4981 gttaataaag gactttcagc agcctcaaga tcggtgatgg tatgcttaaa atggcgagca
     5041 atctcgtgac caaaaccagt cgaaccagtc gaaggataag acttaccacc tgttgtgaca
     5101 atgagtttct cacaagtgaa ggtttgatcc gctgacttaa ggacaaactg atcatctact
     5161 tttttaacag aaacgatttc tatttgagta gcaacttgac cacctagttc ggtgattttc
     5221 ttttccaaag cttcgataat agtccgagac ttgtcactgg ctggaaagac gcgtccgtgg
     5281 tcttcgacct taagtttaac accattttct gtaaaaaagt tgatgatgtc atgattatcg
     5341 aattgggaga aaacactgta aagaaagcgt ccgtttccag gaattccagc tagcaggttg
     5401 tctaagctac cattgttggt cacattgcaa cgtcccccac cagtcccagc taattttttt
     5461 ccaagtttcc gatttttttc gatgaggagg gttttctgtc cataaaagct actggaaata
     5521 gtagccatca taccagcagg tcccccaccg atgacaatag tatcaaaatg tttcatagct
     5581 ctattgtacc acaaaaaaac aagagatgat ggtcacctct tgtcaagaat gcaattaatc
     5641 aatttcatag cccatcagca aaccgccctc ttctgcatag aaactgcaga gaccagaggt
     5701 tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg attcgctctg agagctgttg
     5761 acaacatttt tcgttattgc gttgggccat gacaatacgg ccaccagcat atccagcttt
     5821 tactaactca tcataggcag cttgaactga tttctttgat ccccttgctt tttgtagcaa
     5881 ttcgagagtc ccagtttcac tagcttttcc gaccatacga atgttgagaa ggccaacgac
     5941 cgtaccgata agcttgctca aacggccgtt cttcaccaag ttatcgactt tggctaggac
     6001 aaagagcaac ttagtttttt cttgataggc ggtgatagct tcaaccactt cttcaaaaga
     6061 caagccctgg tcaatcaagt cattcaattt ttctacgagt aggtcaactt caccaccagc
     6121 agataaacta tcaatcacat gaatcttagt gtcaggatgg tcttccagat aaatattctt
     6181 tgctagttga gcactattgt gactgccaga aagggtacct gtgatggtta ctaggaaaat
     6241 gtttttggca ccttcaaatg ctcgcaaata gtcatctggg cttggacaag ctgattttga
     6301 agcttctgca gttgcataca tggtttccat catttggtca atatcgagac tggcgtcatc
     6361 aacaaagacc tgatcagcta cttgaatggt taaggggaca cttacaaagg ttgtgttaat
     6421 agctggtgtt ggcagttgac gataatcaca accagagtca gcaataatct tccaagtcat
     6481 agaaattctc catctttgtc agttatacat tgacaaaggt tctgtctttt tttacaatta
     6541 tatcatgaaa actcttgaaa caaaagcatc atccgtctgt tgtgacaagt agaaagaaaa
     6601 tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa aatctcagaa aatcaaacca
     6661 agaatccaat ttattaacca gagaagccat tgaaacagcc ctcttgcaac tcttggaaaa
     6721 aaaggagctg accaagatta gtatttctga attggtcaaa cgtgcaggtg tttcgcgtgc
     6781 ggccttttat cgcaattatg actccaaaga ggaaatttta gagagcgtct ttaaacgaac
     6841 tgtccacaat attatggaac agctgcatca ttacgattta aagacagacc tttatttggt
     6901 ttgggttcac cttttccggg agaccagaaa ggaagctaga gtaattcaat tggccttgga
     6961 ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa tttctagaaa aataccatgg
     7021 gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg agctcagcca tcgtctctgt
     7081 ccttctaaaa tggatcaagg atggcatgaa ggtaccagct gaaaagattg cagatttagg
     7141 tttaccattt tttaaaaaat agagaaaaag gagaaaagag atgactgaaa aaagactggc
     7201 ctgggatgag tattttgcag cccaagccct actaattgcg aatcgttcca cttgtaaacg
     7261 tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt atttccactg gttacaatgg
     7321 ttcggtgtca gggactgagc attgtattga tcacgaatgt ttggtcattg aaggccactg
     7381 tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa ggtgctgagc gtggtgttcc
     7441 caaaggcttt acagcctatg taacccattt tccttgtctc aactgtacaa aacaattgct
     7501 tcaggtcggc tgcaagcgcg tggtttatat caaccagtac cgaatggacg actacgccca
     7561 atacctttat caagaaaaag ggacagaatt gacccattta ccacttgaga cagtacagac
     7621 agctcttaaa gaggctgatc taatgtaaaa attatcaaaa ataaatggtt tagaaagatt
     7681 tttaaaccgt tttttggtat aataagaaga ataaattgaa agaaggaatt ccaaaaatgg
     7741 gaaaaattga agttattaat cacccactga ttcaacacaa attgtcaatc ttgcgtcgta
     7801 cagatacttc tacaaaagct tttcgtgagc tagtagatga gattgcaatg ttgatggggt
     7861 atgaagtact tcgtgatctt ccactagaag atgtggaaat cgaaacacca attacaaaaa
     7921 cagttcaaaa acaattggca ggtaagaaat tggccatcgt cccaatcttg cgtgcaggta
     7981 tcgggatggt tgatggtctc ttgagcttgg ttccagctgc taaagttggc cacatcggta
     8041 tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt ggtgaaattg cctgaggaca
     8101 ttgaccaacg tcaaattttt gtagtagatc caatgttggc aacaggtggc tcagcaatct
     8161 tggctgttga ttctcttaaa aaacgtggcg catcaaatat caaatttgtc tgccttgtat
     8221 ctgctccaga gggtgtaaaa gtccttcaag aagctcatcc agatgtagaa atctttacag
     8281 cagccttgga tgaacgtttg aacgaacacg gttatatcgt tccaggtctt ggagatgctg
     8341 gagaccgctt gttcggtaca aaataagatc gaaaagaaga atgacttgga aaagtatttc
     8401 cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg aaagcagagc aaaaatttga
     8461 ccttttttga ccaagatatt ataatagtct tatcttcagt catttgacca acaaaattaa
     8521 aactcaaaag gagaaatgaa tgattcctgt agttattgaa caaacaagcc gtggagaacg
     8581 ttcttacgat atttactcac gtcttctcaa agaccgcatc attatgctga caggtccggt
     8641 tgaagacaat atggctaact ctgttattgc ccaattgctt ttcttggatg cccaagatag
     8701 tacaaaagat atttaccttt atgtcaatac accaggtggt tctgtttcag ctggtttggc
     8761 aatcgtagat accatgaact ttatcaaggc agatgtccaa accattgtta tgggaatggc
     8821 tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa ggcaaacgtt tcatgcttcc
     8881 aaatgctgaa tacatgattc atcaaccaat gggcggtaca ggtggtggta cccaacaaac
     8941 tgatatggct atcgctgcag aacacttgct caaaactcgt aataccttgg aaaaaatctt
     9001 ggctgaaaat tcaggtcagt caatggaaaa agtccatgca gatgcagaac gtgataactg
     9061 gatgagcgcc caggaaacac ttgaatatgg ctttattgat gaaattatgg ccaacaattc
     9121 attgaactaa tgatgataga aggcaaactc gactgggttt gctttttttg gtataatagg
     9181 gagagatttc ttagaaagag gatttatcat gtttgaaaaa gtcaatcgct ctggcttgat
     9241 tatctatctt tactataatc gtgatgccaa aaaactgcag gattatggag atattaccta
     9301 tcattccaag aaacatcgtt acttacaact ctatgttcca actcaagaag tggagcaatt
     9361 ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt agggtttgtc atatccaaga
     9421 gttggaaaca ccctttgtgg gcaatcttta tcgataggaa aacgttatca tcgaaaaagt
     9481 tcaaggaaaa cattgacaat tttctgataa ttcggtatat tcttaacaga ctatttaaga
     9541 aataaggaga caaaaagatg aagaaaaaat ttgccctatc gtttgtggcg cttgcaagtg
     9601 tagcacttct tgcagcctgt ggagaagtga agtctggagc agtcaacact gctggtaact
     9661 cagtagagga aaagacaatt aaaatcgggt ttaactttga agaatcaggt tctttagctg
     9721 catacggaac agctgaacaa aaaggtgccc aattggctgt tgatgaaatc aatgccgcag
     9781 gtggtatcga tggaaaacaa atcgaagtag tcgataaaga taataagtct gaaacagctg
     9841 aggctgcttc agttacaact aaccttgtaa cccaatctaa agtatcagca gtcgtaggac
     9901 ctgcgacatc tggtgcgact gcagctgcgg tagcgaacgc tacaaaagca ggtgttccat
     9961 tgctctcacc aagtgcgact caagatggat tgactaaagg tcaagattac ctctttattg
    10021 gaactttcca agatagcttc caaggaaaaa ttatctcaaa ctatgtttct gaaaaattaa
    10081 atgctaagaa agttgttctt tacactgaca atgccagtga ctatgctaaa gggattgcca
    10141 aatctttccg cgagtcatac aagggtgaaa tcgttgcaga tgaaactttc gtagcaggtg
    10201 acacagactt tcaagcagcc cttacaaaaa tgaaagggaa agactttgat gctatcgttg
    10261 ttcctggtta ctataatgag gctggtaaga ttgtaaacca agcgcgtggc atgggaattg
    10321 acaaaccaat cgttggtggt gatggattca acggtgagga gtttgtacaa caagcaactg
    10381 ctgaaaaagc atcaaacatc tactttatct caggcttctc aactactgta gaagtttcag
    10441 ctaaagctaa agccttcctt gacgcttacc gtgctaagta caatgaagag ccttcaacat
    10501 ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa cgcagcaaaa ggtgctaaaa
    10561 attcaggtga aatcaaggat aaccttgcta aaacaaaaga ttttgaaggt gtaactggtc
    10621 aaacaagctt cgatgcagac cacaacacag tcaaaactgc ttacatgatg accatgaaca
    10681 atggtaaagt tgaagcagca gaagttgtaa aaccataata gaaaaatgtt gaaataggga
    10741 atgagccttt gactcactcc ctgtttcgat atttaatact cttcgaaaat ctcttcaaac
    10801 tgcgtcaacg tcgccttgga ttatatatgt gactgacttc gtcagtctta tctacaacct
    10861 caaagcagtg ctttgagcaa cctgtggcta gtttcctagt ttgctctttg attttcattg
    10921 agtataagaa cctatcaaaa agtgagggaa acccctcgga attataaata gaaagagtga
    10981 atcttatgct ccaacaactc gtaaatggtt tgattctagg tagtgtttac gcgctgttag
    11041 ccctaggata taccatggtt tacggaatta tcaagctcat caacttcgcc catggtgata
    11101 tttatatgat gggagccttt atcggttatt tcttgatcaa ttctttccaa atgaatttct
    11161 ttgtagcgct tattgtagct atgctagcga cagctattct tggtgtcgtg attgagtttc
    11221 ttgcttaccg acctttgcgc cactctactc gtattgctgt tttgattacg gctattgggg
    11281 tttctttcct attggagtat ggaatggtct atctggttgg tgccaatacc cgtgccttcc
    11341 ctcaagcgat tcaaacagtt cgatatgatt tgggaccaat tagcttaaca aatgtgcagt
    11401 taatgatttt ggctatttcc ttgattttga tgattttgtt acaagtcatt gtccaaaaga
    11461 ctaagatggg gaaagccatg cgtgcagtat cagtagatag cgacgcggcg caattgatgg
    11521 ggatcaatgt aaaccgtacg attagcttta ccttcgcttt gggttctgct cttgcgggtg
    11581 cggctggtgt tctgattgct ctttattata actctcttga gcctttgatg ggggttactc
    11641 caggtcttaa atctttcgtt gccgcagtac ttggtggtat cggaattatt cctggtgcgg
    11701 ctcttggtgg ctttgtgatt ggtctattgg aaacctttgc gactgccttt gggatgtcag
    11761 atttccgtga tgccattgtt tatggaatct tgttgttgat cttgattgtc cgcccagctg
    11821 gtatccttgg taagaatgtg aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta
    11881 atattctatg gttactcctt ttgttagctg gctatagctt gattagtgta ctggtttcag
    11941 tcggagtact taatctattc tatgtacaga ttttacaaca aattggaatt aatattattt
    12001 tggctgttgg tctcaactta atcgttggtt tttcaggaca attttcactt ggtcatgctg
    12061 gtttcatggc gattggtgcc tatgcagcag ctattattgg ttctacatca ccaacctacg
    12121 gtgccttctt tggagctatg cttgtagggg ctttgctttc aggagtagtt gccttacttg
    12181 tcggcattcc aaccttgcgc ttgaaggggg actatcttgc ggtagcaact ctgggtgttt
    12241 ctgaaattat ccgtatcttt atcatcaatg gtggaagtct tacaaatggt gcggcaggta
    12301 tcttagggat tcctaacttt acaacttggc aaatggttta cttctttgtc gtgattacaa
    12361 ccattgcaac cttgaacttc ttgcgtagcc caattggccg ttcaaccctc tctgttcgtg
    12421 aagatgaaat cgctgctgag tcagttgggg ttaatacgac taaaattaaa atcatcgctt
    12481 ttgtctttgg tgccattact gcaagtattg ctgggtcact tcaggcagga tttatcgggt
    12541 ctgttgtacc gaaagattac accttcatca actcaatcaa cgttttgatt attgttgtat
    12601 ttggtggact cggttccatt acaggtgcga ttgtttcggc tattgttctg ggaattttga
    12661 atatgcttct ccaagatgtt gctagtgtgc gtatgattat ttacgctttg gccttggtat
    12721 tggtaatgat tttcagacca ggtggactcc ttggaacatg ggaactgagc ctatcacgtt
    12781 tctttaaaaa atctaagaag gaggaacaaa actaatggca ttacttgaag taaaacagtt
    12841 aaccaaacat tttggtggtc taacagctgt tggagatgtg actcttgaat tgaacgaagg
    12901 ggaactggtt ggattaatcg gtccaaacgg agctgggaaa accacccttt tcaacctttt
    12961 gaccggtgtt tatgaaccaa gcgagggaac agtaacccta gatggtcacc ttttgaatgg
    13021 gaaatcacct tataagattg cctctttggg acttggacgt actttccaaa atatccgtct
    13081 ctttaaagat ttaacagttt tagacaatgt tttgattgct tttggaaacc atcacaaaca
    13141 gcatgttttt actagtttct tacgcttacc agctttttac aagagtgaaa aagaattaaa
    13201 ggctaaagct ttggaattgt tgaaaatctt tgatttagat ggtgatgcag agactcttgc
    13261 taaaaatctt tcctacggac aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga
    13321 acctaaaatt ctcttcttag atgaaccagc agcaggtatg aacccacagg aaacagccga
    13381 attgactgag ttaattcgtc gtatcaaaga tgaatttaag attacaatca tgttgattga
    13441 acacgatatg aatctggtca tggaagtaac agaacgtatc tacgtacttg aatatggccg
    13501 tttaattgct caaggaactc cagacgaaat taagaccaat aaacgcgtta tcgaagctta
    13561 tctaggaggt gaagcctaat gtctatgtta aaagttgaaa atctttctgt gcattacggt
    13621 atgatccaag cagtccgtga tgtaagcttt gaagttaata aaggagaagt tgtttccctt
    13681 atcggtgcca atggtgcagg taagacaact attcttcgca ccttgtcagg tttggttcga
    13741 ccaagttcag gaaagattga atttttaggt caagaaatcc aaaaaatgcc agctcagaaa
    13801 atcgtggcag gtggtctttc acaagttcca gaaggacgcc acgtctttcc tggcttgact
    13861 gttatggaaa atcttgaaat gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct
    13921 aacttgaaga aggttttctc acgctttcct cgtcttgaag aacggaagaa ccaagatgca
    13981 gctactcttt caggagggga acaacaaatg cttgccatgg gacgcgctct tatgtcaaca
    14041 ccaaaacttc ttcttttaga tgaaccatca atgggacttg ccccaatctt catccaagag
    14101 atttttgata tcattcaaga tattcagaag cagggaacaa ccgtcctctt gattgaacaa
    14161 aatgccaata aagcacttgc aatctctgac cgaggatatg tattggaaac aggaaaaatc
    14221 gtcctatcag ggacaggaaa agaactcgct tcatcagaag aagtcagaaa agcatatcta
    14281 ggtggctaaa acaatccaga ggattgtttt agccggcaga tggagattac gaagtaatca
    14341 tcaatatagt ccgggggacc tttttagtcg gtagattgag attgcaaaca aatatgcatc
    14401 tacattgaaa gcttaatttc taataattga aaaaatcgaa tgaaaaattt cttaccttca
    14461 ttcacagagc tctatttcag agctcttttt gctagcttat tcatactttt ctgaatttcg
    14521 aaaaagaaat gtaagcgttt gatagatttg caaaaagatt gtataatagg gataagaata
    14581 gaaaaggaga agtctcatgg cagttaaaga ttttatgacc cgcaaggtag tttatattag
    14641 tccagataca acagtatctc atgcagcaga tttgatgaga gagcaaggtt tgcaccgtct
    14701 gcctgttatc gaaaatgatc aattagttgg tttggtgact gagggaacca ttgcacaagc
    14761 aagtccatct aaagcaacaa gtctttctat ctatgagatg aattatcttc tgaataagac
    14821 aaaagtaaaa gatgtcatga ttcgcgatgt tgtcactgtc tcaggctatg ctagtctaga
    14881 agatgcaact tatctgatgt tgaaaaataa gattagtatt ctccctgtcg tagataacca
    14941 tcaagtatac ggagttatta ctgaccgtga cgttttccaa gcctttcttg aaattgcagg
    15001 ttatggcgaa gaagggattc gtgtacgctt tgttacagaa gatgaagttg gtgttcttgg
    15061 aaaaattgtt tctttgattg tagaagaaaa tttgaatatc tcccatacag tcaatattcc
    15121 gcgtaaggat ggtaaggtga ttatcgaagt gcaaatcgat ggatcaattg atttaccagc
    15181 cttgaaagaa aaatttgaag caaatggtat tcaagtggaa gaaatcgctc gcacttcagc
    15241 aaaagtcttg taagaaggga agcccaaagg cttctttttt catgaaaagg ggattagaga
    15301 aaaagatgga aagaaatgat aaaatatgct ataatgaaat agtgtaaaaa aggagtattt
    15361 atggacattt cagtaattcg tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc
    15421 agggggtctc tttgacctcg aagggctaga ggaagagatt gccatcttgg aaaacaagat
    15481 gacagaacct gatttttgga acgataatat tgcggcccaa aaaacgtcgc aagaattaaa
    15541 tgaattaaaa aacacttaca ataccttcca taagatggaa gagttgcagg atgaagtcga
    15601 aattttattg gattttttgg ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt
    15661 agccgaactt gataagataa tgaccagcta cgagatgact ctactcttgt cagaacctta
    15721 tgaccacaac aatgccatct tggaaatcca tccaggttct ggtggtactg aggcgcagga
    15781 ctggggtgat atgttgcttc gtatgtatac tcgttatggt aatgctaaag gctttaaagt
    15841 ggaagtgttg gattaccaag caggtgatga ggctggtatt aagtcggtaa ctttatcatt
    15901 tgaagggcct aatgcctatg gtctcctcaa gtcagaaatg ggtgttcatc gcttagtgcg
    15961 aatctcacca tttgactctg ccaaacgtcg ccatacctct ttcacatctg tagaagtgat
    16021 gccagaattg gatgatacta ttgaagtgga aatccgtgaa gatgatatca agatggatac
    16081 cttccgttca ggtggtgccg gtggacaaaa cgtcaataaa gtttcaacag gtgtacgttt
    16141 aacccacatt ccaactggaa ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa
    16201 tagagatcgt gccatgaaga tgttgcaggc taagctctat caaatggagc aagataagaa
    16261 ggctgcggag gtagattctc tcaaaggtga gaaaaaggag atcacttggg gaagccaaat
    16321 ccgttcttat gtcttcacgc cttatactat ggtaaaagat caccgaacta gctttgaggt
    16381 tgctcaggta gataaggtta tggatgggga cctagatggt tttatcgatg cttatctcaa
    16441 gtggcgaatt agctaagata gaaaggaact cacatgtcaa ttattgaaat gagagatgtc
    16501 gttaaaaaat acgacaacgg aacgactgct ctacgcggtg tttcggtcag cgttcaaccg
    16561 ggggaatttg cttacatcgt aggaccttca ggagcaggga agtcaacttt tattcgttct
    16621 ctgtatcgtg aagtaaaaat cgataaagga agcctatcag ttgctggttt taatctggtt
    16681 aagatcaaaa agaaagatgt cccgcttcta cgtcgtagtg ttggggttgt cttccaggat
    16741 tataaattgt taccaaagaa aactgtctat gaaaatattg cttacgctat ggaagtaatc
    16801 ggggaaaatc gccgtaatat caaaaaacga gtgatggaag ttttggactt ggttggattg
    16861 aagcataagg ttcgttcttt cccaaatgaa ctctcaggtg gggagcaaca gcggattgcg
    16921 attgcgcgtg caattgtaaa taatcccaaa gtattgatag ctgatgagcc aacaggaaat
    16981 ctggatccgg ataattcatg ggaaattatg aatctcttgg aacggattaa cctacaaggg
    17041 acaactattt tgatggcgac tcataatagc cagattgtaa ataccttgcg ccaccgtgtc
    17101 attgccattg aaaatggccg tgtcgttcgt gacgaatcaa aaggagagta tggatacgat
    17161 gattagtaga ttttttcgcc atttatttga agccttaaaa agtttgaaac gaaatggttg
    17221 gatgacagta gctgctgtca gttcagtcat gattactttg accttggtgg caatatttgc
    17281 atctgttatt ttcaatacag cgaaactagc tacagatatt gaaaataatg tccgtgtagt
    17341 agtttatatc cgaaaggatg tggaagataa tagtcagaca attgaaaaag aaggtcaaac
    17401 tgttacaaat aatgactacc acaaggtata tgattctttg aagaacatgt ctacggttaa
    17461 aagtgttacc ttttcaagta aagaagaaca atatgaaaaa ttaaccgaga taatgggaga
    17521 taactggaaa atctttgaag gagatgccaa tcctctctat gatgcctata ttgtagaggc
    17581 aaacgctcca aatgatgtaa aaactatagc cgaagatgct aaaaaaattg aaggtgtctc
    17641 tgaggttcaa gatggcggtg ccaatacaga aagactcttc aagttagctt catttatccg
    17701 tgtttgggga ctagggattg ctgctttgtt aatttttatc gcagttttct tgatttcaaa
    17761 taccattcgt attaccatta tttcccgcag tcgcgaaatt caaatcatgc gcttggtcgg
    17821 agctaaaaac agttatatcc gtggaccgtt cttgttagaa ggagccttta tcggtttatt
    17881 gggagctatc gcaccatctg ttttggtctt tattgtttat caaattgttt accaatctgt
    17941 caacaaatcg ttggtagggc aaaatctatc catgattagt ccagatttat ttagtccgtt
    18001 gatgattgcc ctactatttg tgattggggt tttcattggt tcattgggat caggaatatc
    18061 catgcgccga ttcttgaaga tttaggtaaa atagctgctt ttatgaggag attgtaaaat
    18121 ctcctttttt gctacaagag tttttgaaaa gagatgcgta gaagaaaaga gcttccaaag
    18181 aagtccccca gagaagactt cttgatggct aatttgaact tgaaatttgg cgcactagaa
    18241 attttttaga aaaaagtgta atttttttat aaaagccttt acaaaaaaaa ttaaagtggt
    18301 ataattaaat catagaaggt gcaaacgttt tcgtaaagcg tttgcctaaa taaaaataaa
    18361 ggagatttga atgatgaaag atacattcaa aaatgtcttg tctttcgaat tttggcaaaa
    18421 attcggtaag gctttgatgg tagttatcgc ggttatgccg gctgctggtt tgatgatttc
    18481 aatcggtaag tctatcgtga tgattaaccc aacctttaca ccacttgtca tcacaggtgg
    18541 aattcttgag caaatcggtt ggggggttat cggtaacctt cacattttgt ttgccctagc
    18601 cattggagga agctgggcta aagaacgtgc tggtggtgct ttcgccgctg gtcttgcctt
    18661 tatcttgatt aaccgtatca ctggtacaat ctttggtgtg tcaggcgata tgttgaaaaa
    18721 tccagatgct atggtaacta ctttctttgg tggttcaatc aaagttgctg attactttat
    18781 cagtgttctt gaagctccag ccttgaacat gggggtattc gtagggatta tctcaggttt
    18841 tgtaggggca actgcttaca acaaatacta caacttccgt aaacttcctg atgcactttc
    18901 attcttcaac gggaaacgtt tcgtaccatt tgtagttatt cttcgttcag caatcgctgc
    18961 aattctactt gctgctttct ggccagtagt tcaaacaggt atcaataact tcggtatctg
    19021 gattgccaac tcacaagaaa ctgctccaat tcttgcacca ttcttgtatg gtactttgga
    19081 acgtttgctc ttgccatttg gtcttcacca catgttgact atcccaatga actacacagc
    19141 tcttggtggt acttatgaca ttttaactgg tgcagctaaa ggtactcaag tattcggtca
    19201 agacccacta tggcttgcat gggtaacaga ccttgtaaac cttaaaggta ctgatgctag
    19261 tcaataccaa cacttgttag atacagtaca tccagctcgt ttcaaagttg gacaaatgat
    19321 cggttcattc ggtatcttga tgggtgtgat tgttgctatc taccgtaatg ttgatgctga
    19381 caagaaacat aaatacaaag gtatgatgat tgcaacagct cttgcaacat tcttgacagg
    19441 ggttactgaa ccaatcgaat acatgttcat gttcatcgca acacctatgt atcttgttta
    19501 ctcacttgtt caaggtgctg ccttcgctat ggctgacgtc gtaaacctac gtatgcactc
    19561 attcggttca atcgagttct tgactcgtac acctattgca atcagtgctg gtattggtat
    19621 ggatatcgtt aacttcgttt gggtaactgt tctctttgct gtaatcatgt actttatcgc
    19681 aaacttcatg attcaaaaat tcaactacgc aactccaggg cgcaacggaa actacgaaac
    19741 tgctgaaggt tcagaagaaa ccagcagcga agtgaaagtt gcagcaggct ctcaagctgt
    19801 aaacattatc aaccttcttg gtggacgtgt aaacatcgtt gatgttgatg catgtatgac
    19861 tcgtcttcgt gtaactgtta aagatgcaga taaagtagga aatgcagagc aatggaaagc
    19921 agaaggagct atgggtcttg tgatgaaagg acaaggggtt caagctatct acggtccaaa
    19981 agctgacatt ttgaaatctg atatccaaga tatccttgat tcaggtgaaa tcattcctga
    20041 aactcttcca agccaaatga ctgaagcaca acaaaacact gttcacttca aagatcttac
    20101 tgaggaagtt tactcagtag cagacggtca agttgttgct ttggaacaag taaaggatcc
    20161 agtatttgct caaaaaatga tgggtgatgg atttgcagta gaacctgcaa atggaaacat
    20221 tgtatctcca gtttcaggta ctgtatcaag catcttccca acaaaacatg cttttggtat
    20281 tgtgacggaa gcaggtcttg aagtattggt tcacattggt ttggacacag taagtcttga
    20341 aggtaaacca tttacagttc atgttgctga aggacaaaaa gttgcagcag gagatctcct
    20401 tgtcacagct gacttggatg ctatccgtgc agcaggacgt gaaacttcaa cagtagttgt
    20461 cttcacaaat ggtgatgcaa ttaaatcagt taaattagaa aaaacaggtt ctcttgcagc
    20521 taaaacagca gttgctaaag tagaattgta atatacttga ggttggaagc tgtattccaa
    20581 cctcttattt tgggagaaaa gaatgaaatt tttaacactc aatactcaca gttggatgga
    20641 gaaagaagca gaggaaaaat tccagatttt gcttgaagat attcttgaaa aggactatga
    20701 tttgatttgt ttccaagaaa tcaatcagga gatgacctcg tcagaggtgg aggttaatga
    20761 cctttatcaa gctttgccag cagctgagcc tattcaccaa gaccattatg ttagactctt
    20821 ggttgaaaag ttgttagagc aagggaaaaa ttactactgg acctgggctt ataaccatat
    20881 cggctatgac cgctaccatg aaggtgtggc tatcttgtct aaaacaccta ttgaagccag
    20941 agaaattttg gtttcggatg tggatgatcc aacagactat catactcgcc gtgttgccct
    21001 agctgaaact gtagtcgatg gcaaggagct agcagttgcc agtgttcatc tctcttggtg
    21061 ggataaaggt ttccaagaag aatgggcacg atttgaggct gtcttgaaaa aattgaacaa
    21121 gccactttta ctagctggag attttaacaa cccagcaggt caggaaggtt accaagctat
    21181 tttagctagt ccattaggct tacaagacgc atttgaagtt gctcaagaga aaagtggtag
    21241 ctatactgtt ccgcctgaaa ttgatggctg gaaagagaac actgaacccc ttcgaatcga
    21301 ttatgtcttt actaccaaag agttagcggt ggaaaattta catgtcgtat ttgatggtaa
    21361 caagagtcca caagtgagtg atcactatgg cttgaatgct atattaaact ggaaataata
    21421 actgaaaaga ggttggaact ataaaattct agccttttct tactagagaa gctactggaa
    21481 atagcctaaa taagtgagac tactgtaatg gaataaaata tggtataatt gataagatag
    21541 atagaatcga ggatgttatg tcatttacga aatttcaatt taaaaactat attagagaag
    21601 ccttgaagga gttaaaattt acaactccaa cagaggtgca agataagttg attcctattg
    21661 ttttggcagg tcgtgaccta gtaggagaat caaaaacagg ttcaggtaag actcatactt
    21721 tcttgttacc gattttccag caattagatg aagctagcga tagtgtacaa gcagtgatta
    21781 ctgcaccgag tcgtgagttg gctactcaaa tttaccaagt agcgcgtcag atttcagctc
    21841 actcagatgt cgaagttcgt gtggttaatt atgtgggtgg tacggataag gctcgccaga
    21901 ttgagaaatt ggcaagcaat cagcctcata ttgttattgg aacaccaggc cgtatctacg
    21961 acttggttaa atctggtgat ttagctattc ataaagccaa gacatttgtt gttgatgaag
    22021 cagatatgac cttggatatg ggattcttgg aaattgttga taagattgct ggcagtcttc
    22081 caaaagactt gcaattcatg gtcttctcag cgactatccc acaaaaactg caaccattct
    22141 tgaaaaaata cttatcaaat cctgttatgg agaaaattaa gactaaaacg gttatttctg
    22201 acaccattga taattggttg atttcgacca agggacatga taagaatgct caaatttacc
    22261 aattgactca gttgatgcag ccgtatttgg caatgatttt tgttaacact aaaacgcgtg
    22321 ctgatgaatt gcattcatat ctgactgctc aaggcttgaa ggttgcaaaa atccatggag
    22381 atattgcccc tcgtgaacgc aagcgaatca tgaatcaggt gcaaaatctg gattttgagt
    22441 atattgtcgc aacagattta gcagcgcgtg ggattgacat tgaaggtgtc agccatgtca
    22501 tcaatgatgc cattccgcaa gacttatctt tttttgttca ccgtgttggt cgtactggac
    22561 gaaatggcct accaggtaca gctattaccc tttatcagcc aagtgatgac tcggatatcc
    22621 gtgagttgga gaaattggga atcaagttta gtcctaagat ggtcaaagat ggggaatttc
    22681 aagataccta tgaccgtgat cgtcgtgcca accgtgagaa aaaacaagat aaacttgata
    22741 tcgaaatgat tggtttggtt aaaaagaaaa agaaaaaagt caaaccgggt tataagaaga
    22801 aaattcaatg ggcggttgat gaaaagcgcc gtaaaactaa gcgtgctgaa aatcgcgctc
    22861 gcggtcgtgc agagcgtaaa gctaaacgcc aaacatttta atagaaattg ttggagtact
    22921 gagctccaac ttttttattt atgagaacga actatctaaa ccgaaacact acattaaaga
    22981 ctgcaaattg cgattaaaaa tggtataatg ataaagttat atagtcccga taagatggta
    23041 ggtatttatt acgaagagtt ttcctatcag tagtttgcaa ttcgcaaaga atgacggctt
    23101 cgaggtcgct cgtctttcta cggttgccgc catactgcgg tcaccctatg cgccttacta
    23161 gcttcagaaa taaaaaagta tcgtttttat ttctgaagct agtaactctt agataatgga
    23221 aattcctatc agtactttgt aactctataa caatattttt taagggggga catttttatg
    23281 tcagaacgta aattattcac gtctgaatct gtatctgagg ggcatccgga taagattgca
    23341 gaccaaattt cagatgcgat tttggatgct attttagcaa aggatccaga ggcgcacgtt
    23401 gctgctgaaa cagctgtata tactggttct gtccacgttt ttggtgaaat ttctacaaat
    23461 gcctatgtgg atattaaccg tgtggttcgt gataccattg cagagattgg ttataccaat
    23521 acagaatatg gattttctgc tgagacggtg ggagtacacc catctttggt ggaacaatct
    23581 cctgacatcg ctcaaggtgt taacgaagcc ttggaggttc gtggaaatgc tgatcaagat
    23641 ccactggact tgattggagc aggtgaccaa gggctcatgt ttggatttgc agtagatgaa
    23701 acagaagagc ttatgccatt gccaattgca ctcagtcata aattggttcg tcgtctggca
    23761 gaacttcgta agtctggaga aattagctat ctccgtccag atgcaaaatc acaagttaca
    23821 gttgagtacg atgaaaatga ccgtccggta cgtgtagata cagtcgttat ttctactcag
    23881 catgatccag aggccactaa tgaacaaatc catcaagatg tgattgacaa ggtcatcaaa
    23941 gaagttattc catcttctta tcttgatgat aagacaaaat tctttatcaa tccgacaggt
    24001 cgttttgtaa tcggtggtcc tcaaggggac tcgggtttga ctggtcgtaa gattattgta
    24061 gatacttatg gtggctactc tcgtcatggt ggtggtgcct tctctggtaa agatgcgact
    24121 aaggtggatc gttcagcctc ttatgcggct cgctatattg ccaagaatat cgttgcagca
    24181 ggccttgcta agaaggcaga agtgcagttg gcctatgcta tcggtgttgc gcaacctgtt
    24241 tctgttcgta tcgatacttt cggtacagga acagtagctg aaagtcaact tgaaaaagcg
    24301 gctcgtcaaa tctttgacct tcgccctgca gggattatcc aaatgctgga ccttaagcgt
    24361 ccaatttacc gtcaaacatc ggcttacggt cacatgggac gtacagatat tgatcttcca
    24421 tgggaacgtt tggataaggt agatgctttg aaagaagcag taaaataaga ttttaagagg
    24481 ggaacgtcct ctctttttta tagtttttaa ctatattggg atactgttct gaaaatccat
    24541 tttgcgaaag tagagattta catgtatagt agattgaaac tagaatagta cacttcaact
    24601 tctaaaacat tgttagaaat cgatttgact gtcctgaacg atttctcctg ttcttgtttc
    24661 attttactat atttcttt
//
LOCUS       DAVKWS010000025        23775 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKWS010000025 DAVKWS010000000
VERSION     DAVKWS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.025"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(98..2356)
                     /locus_tag="VW852_001135"
     CDS             complement(98..2356)
                     /locus_tag="VW852_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0272293.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VW852_001136"
     CDS             complement(2585..2815)
                     /locus_tag="VW852_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV0272294.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VW852_001137"
     CDS             3005..3688
                     /locus_tag="VW852_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0272295.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VW852_001138"
     CDS             3688..4422
                     /locus_tag="VW852_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0272296.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VW852_001139"
     CDS             4576..5433
                     /locus_tag="VW852_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV0272297.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VW852_001140"
     CDS             5575..6447
                     /locus_tag="VW852_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV0272298.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPESMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VW852_001141"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VW852_001141"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV0272299.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VW852_001142"
     CDS             7426..8637
                     /locus_tag="VW852_001142"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV0272300.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEADIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VW852_001143"
     CDS             8639..9181
                     /locus_tag="VW852_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV0272301.1"
                     /translation="MTRYALLVRGINVGSKNKVVMAELHQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="VW852_001144"
     CDS             9197..10006
                     /locus_tag="VW852_001144"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0272302.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKIDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10482..10877
                     /locus_tag="VW852_001145"
     CDS             10482..10877
                     /locus_tag="VW852_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV0272303.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10887..11462
                     /locus_tag="VW852_001146"
     CDS             10887..11462
                     /locus_tag="VW852_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV0272304.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKARFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDGSGKEIE"
     gene            11487..11999
                     /locus_tag="VW852_001147"
     CDS             11487..11999
                     /locus_tag="VW852_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV0272305.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12653..13363
                     /gene="deoD"
                     /locus_tag="VW852_001148"
     CDS             12653..13363
                     /gene="deoD"
                     /locus_tag="VW852_001148"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0272306.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13386..13910)
                     /locus_tag="VW852_001149"
     CDS             complement(13386..13910)
                     /locus_tag="VW852_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV0272307.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13963..14199)
                     /locus_tag="VW852_001150"
     CDS             complement(13963..14199)
                     /locus_tag="VW852_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV0272308.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(14267..15187)
                     /locus_tag="VW852_001151"
     CDS             complement(14267..15187)
                     /locus_tag="VW852_001151"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV0272309.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTVTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15377..15780
                     /locus_tag="VW852_001152"
                     /pseudo
     CDS             15377..15780
                     /locus_tag="VW852_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15996..16577
                     /locus_tag="VW852_001153"
     CDS             15996..16577
                     /locus_tag="VW852_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0272310.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16574..17851
                     /locus_tag="VW852_001154"
     CDS             16574..17851
                     /locus_tag="VW852_001154"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV0272311.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRSLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17869..18531
                     /gene="deoC"
                     /locus_tag="VW852_001155"
     CDS             17869..18531
                     /gene="deoC"
                     /locus_tag="VW852_001155"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV0272312.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18518..18907
                     /locus_tag="VW852_001156"
     CDS             18518..18907
                     /locus_tag="VW852_001156"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0272313.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            18998..20050
                     /locus_tag="VW852_001157"
     CDS             18998..20050
                     /locus_tag="VW852_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV0272314.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20194..21729
                     /locus_tag="VW852_001158"
     CDS             20194..21729
                     /locus_tag="VW852_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272315.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRCG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            21722..22780
                     /locus_tag="VW852_001159"
     CDS             21722..22780
                     /locus_tag="VW852_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272316.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22783..23739
                     /locus_tag="VW852_001160"
     CDS             22783..23739
                     /locus_tag="VW852_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272317.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6906 a         4305 c         5421 g         7143 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttatca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata tggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggggcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggcccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatctgtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt cgtccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag cttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc aacaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaggaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac tattactcaa
     4801 gaggaattgt tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg
     4861 gtccagttgc cattaccaaa acatatcgat gaagaggcgg ttttattagc cattgaccca
     4921 gaaaaggatg tggatggttt ccatccccta aacatgggac gtctttggtc tggacatcca
     4981 gtcatgattc cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac
     5041 ttggaaggta aaaatgcggt cgtaatcggt cgttcaaata tcgttggaaa acctatggct
     5101 cagcttcttt tggctaaaaa tgcgacagta accttggccc actcacgaac tcataatctt
     5161 gccaaggtgg ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa tcgagatgaa
     5281 aatgggaagc tctgtggaga tgttgattat gaggcagttg caccacttgc tagccatatc
     5341 acgccagtcc ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaac attcgattaa tagagtgtat
     5461 tttctatagc tatatctaaa atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa
     5581 gtgattaatc aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggctctgca cagccattta cctgagtcta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagttg tcttgctggg gcctggttta
     5881 cgagacgatg cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagag
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaattgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgca gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaatcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aattgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctgatat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt agtaagaata aggtcgtcat
     8701 ggcggagctt catcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 cagtggcaat attttctttg cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca gaagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt gggtcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caagggcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcaga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatggggg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcctac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagattgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggctggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aagggggagt gcctctgtct tttcaggatt gactgcctat ccgtattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtgtgt
    10141 ctgatctgat agccagcatt gtgaaagaca agattctagg atactagcat tagcttccta
    10201 gccaagcaga ctagtatgat aaggagagat gagaatgaat tgactttctg aatttctcag
    10261 tcttatcata tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga
    10321 aagataagaa ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt
    10381 atcggagttg tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca
    10441 atgaagtaaa gcaagatgta acatctgaag tggtaatagg tgtgctagat tctaaggagg
    10501 aattgaaaga gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa
    10561 gactagctcc tcaaacgctt ccggaagcaa gtgaagttct tgaaaacaaa agggaagagt
    10621 caaaagtaga gataacagag ccagctcaag cggatgatat ccgcaaggtt gttggggaat
    10681 tagccaagga tataagtatt actaagttgt atatgacagg tcattctctt ggaggctacc
    10741 tagctcagat tgcagcggtt gaagattacc aaaaatatcc tgatttttat aaccatgtat
    10801 tgaggaaagt gacaactttc agtgctccta aagtgattac ttccagaact gtttgggatg
    10861 ctaagaatgg tttctgagat gttggtttgg aaagtcgtaa attagctgtt agtggaaaaa
    10921 ttaagcatta tgtggttgat aatgacaatg ttgtgactcc cttgattcat aataatcgtg
    10981 atattgttac atttacaggt aattcacgct ttaaacaccg ttctcgtggc tattttgaaa
    11041 gtccaatgaa tgatattcct aactttaata ttggtaaaca agctaccttg gataaacatg
    11101 gttatcgtga tccgaaattg gataaagcgc gattctttaa gaaacaggct ctacctcaat
    11161 cttctagtca accaagcgct gaaccaatgg aaaatattgc cttaggaaaa caggttactc
    11221 aaagttcgac agctttcgga ggagatgcta gaagagctgt ggatggcaaa gtcgatggta
    11281 actatggtca caattctgtc actcatacaa acttccaatc taagccttgg tggcaagtag
    11341 atttggctaa agaagaaacc attcgccaaa tcaatattta caaccgaaca gacactgccc
    11401 aggatagatt ggcaaacttt gatgtcattc ttttagacgg ttctggtaaa gaaattgagt
    11461 gaaaacgtat aatatctcct aaagatgtgt cagcacaaat tacgattaac cataaaaaag
    11521 cgcgctatgt tcggattgag ctagaaggct ataatgccct cagtcttgca gaagttgaag
    11581 ttttccgctt tatagctacg aatggtgaaa cggcgataca agtttctaag tcagttcaac
    11641 caatcagtca gactcctgtg aaagataaaa cattgacaat tcaacacagt ggagcttaca
    11701 ttgcccgcta ctccataact tgggaagaag ttccagtaga taaagatgga aaccaagttg
    11761 ttcgtagtca ttcttgggaa ggaaacggtc gcaaccagac tgcaggtttt gtcctcaacc
    11821 tcccaatcaa agaaaatatg agaaatctgc gagttaagat tgagaaaaag acgggcctac
    11881 tatggaatag atggcaaaca atctatgaaa acagaccaat tttagctcaa ccccaccgta
    11941 aaattaccca ttggggtacg acattgaatt ccaaggtgag tgacgatgat gtcttgtaat
    12001 ctgatggtac aatgacagtt agtttgtcta gtttataaga aagtactacc tgagcttgaa
    12061 tagaactcag gtagctctct gtgaaagaac aaaattaata ctcaatgaaa atcaaagagc
    12121 aaactaagaa actagccgca ggttgctcaa agcactgctt tgaggttgta gataagactg
    12181 acgaagtcag tcacatatat aatccaaggc gacgttgacg tggtttgaag agattttcga
    12241 agagtataaa cagaaaggta gagcgcgtgt tctaatttga acacgagtag aaaacttttc
    12301 taaaaacaaa aacgaaagga tgggtaaact gtattcgctg aactgaatac gggcgactct
    12361 cctctaaatc aaaattaaga aaggaattga ccccacccta aaagtagtgg gaaaaagata
    12421 gttggtctag cgagcatcgc tcactgcgcc caactcctat tttcccttcg ctttttgatg
    12481 ggtttggtat ctttctcaat ataaaatata aaataaagaa aggtagagcg tgtgttttga
    12541 tttgaacacg agcggaaaac tcggaaaata gataatctga ctgaaaaatc aggatttctc
    12601 gtcaggttcc taattttcag tcgttttctt ctcgctcttt gtatcataaa ttatgtctat
    12661 ccatattgct gctcagcagg gtgaaattgc tgataaaatt cttcttcctg gggatcctct
    12721 tcgtgctaag tttattgcgg agaatttcct tggtgatgct gtttgtttta acgaagtgcg
    12781 taacatgttt ggttacactg gtacttacaa gggtcaccgt gtatctgtca tgggaactgg
    12841 gatgggaatg ccatctattt cgatttatgc gcgtgagtta atcgtagact acggtgtgaa
    12901 gaaattgatt cgtgtgggaa ctgcaggttc tttgaatgaa gaggttcatg ttcgtgaatt
    12961 agttttggcg caggcggctg caaccaactc aaacatcgtt cgtaatgact ggccacagta
    13021 cgattttcca caaattgcta gctttgattt gcttgataaa gcctaccata tcgccaaaga
    13081 acttggtatg actactcacg ttgggaacgt tttgtcatct gatgtctttt actcaaatta
    13141 ctttgaaaag aatatcgagc ttggtaaatg gggagtcaag gctgtggaaa tggaagcagc
    13201 agctctttac tatcttgctg cccaatacca tgttgatgcg ctagctatca tgaccatctc
    13261 tgatagcttg gtcaatccag acgaagacac aactgcagaa gaacgtcaaa ataccttcac
    13321 tgatatgatg aaggttggtt tggaaacctt gattgcagaa taattatagc caaaaagaaa
    13381 gccctttatg aattgtagga cttccttttc ttatccagaa attgatctag ctctctctga
    13441 tttcgaagaa tagtgacttt atgtgaatat tcttggcaaa gtttttggta attttctttt
    13501 tgagttttgc tacgcccatc ccaaagaatc catctgataa actcccagtc aaagcgttca
    13561 gggcaatctg ccgccatact ttctctgact tttccacggt atttaagata acgcttaaag
    13621 gctctaaaga gacaggtcaa tggcaaaaaa ttgagaaaga tgatttggtc agcttcttgc
    13681 attcgttctt ggtagtagca ccaagaataa ttaccatcga tgacccaagc tttatgcttg
    13741 gtgagaaagt tttttatctc ggttaacatc cattcgcagt cactgtcttg ccaaccaggt
    13801 tgaaattgga gtgtgtccat gtgcagtttt ggaatggagt agtagttaga taacttttct
    13861 gctagagttg acttaccaga accagaatat ccgataattg tgattttcat tttctacctt
    13921 ttcctatttg gagacaaaaa aacagcctct atggactgtt tcttatttag caagtttagt
    13981 tgaaagacga gctttatcgc ggcttgcttt gtttttgtga atcaaacctt tagtttctgc
    14041 tttatcgata gctgagctag cagcacggaa aagttcttca gatgggtttg cttcgaaagc
    14101 ttttatagca gtacgcatag ctgatttttg agctgagttc ttttcgtttt gtttaacgtt
    14161 caattcagcg cgtttgatag ctgatttaat gtttgccaat gttcttacct ccatatttac
    14221 taactatacc attatatatg aaaacttacg ttttgacaag ggaaaattac ttttttaagt
    14281 aaatttcatc gatttcatgg ttcttgcttt tatgaagaat cacttctgca cgatttctgg
    14341 ttggttcaat ataattttgc agatttgtga gattgatact gatccagacc tgatgggcaa
    14401 aggactccac ttccccaatc ggcatctgag taaaacgata atagtagcta tcagggtcgt
    14461 tttgggctag gctcagcatc ttcaagaaac ggtccagata ccaactttca atatcatcca
    14521 ctccagcatc tacatagatg gaaaagtcaa agaagtcagt gatatagaga cgatcgtttt
    14581 gtggattttg aaagacatta attccctcaa caattacaaa atcagcagct ttgacacttt
    14641 gtttcttttc gggtacgatg tcgtaaactt catgagaata gacaggaata tctacatctt
    14701 gtccattttt gatgcggtcc aagaagttga gaagagcttc catatcatag ctttcaggaa
    14761 atcctttacg atttaaaatc ccctgctcaa tcaaggtttg attgggatag agaaaaccat
    14821 cagttgtaac caactcaacc gtagcatctg taaccgtacg ggacagtagg atttgaagta
    14881 ggcgactggt tgtggatttt ccaacggcaa cactcccaga aaccccaata ataaaaggtt
    14941 gagatttact ttcacgttgg aggaaaattc cttttgaaaa ggctaaatct tccttagttc
    15001 gcttgtaaat ctgaatcaga tgagccaagg ggagatagat atctgtaacg tcttggagac
    15061 tgatttggtc attaaaactc ttgatagatt ccaattcttc ttctgtcaaa ggaggtgttg
    15121 tctttcgatg taaagattgc caagtctggc ggctgatttt ttcaaaatgt aaaaattcgt
    15181 tggtcatttg ttttccccta gatattttgt ctatcatacc ataaaaagct aaaaaaataa
    15241 agcggtttct tgaatgtggt aagtcaatgg tatatagtat aagtgatcaa tttattgatg
    15301 aaataggaga tagattctac tgatttataa gcttataaag ggggcttttt agttcacaac
    15361 ttataggagg tgtactatga aaatcttaaa acgttacata ttggaactct gttttatttt
    15421 aagttttgct ttacctttta taaaaggaac caatgcagat aatggtagat gctttgtgga
    15481 aacctattac ggttttactt ttttgatgga acatgctatt gtaacagctg tctttatctg
    15541 ttcgttctta attgctttct tactaaaaaa cgatggacga aatggattgc tgcgggtagt
    15601 tattgctttt tagtcctacg gattgcgaca gagggttatt tcttccacat gtcactagaa
    15661 gatttgatac gactttggac aagtttggaa atcctgacac aaacgtatca gttgggcttt
    15721 tatctaaaca ttttgttagg aattttgttg ataataaagt attttaaggt taagcaatag
    15781 aaataataat ctgcctgatt atagatgata aactttttgt ttttatctgg actgtgatat
    15841 ttatagttct aaatggttta ttatttataa gtgttgaaag agattgaaga aaatggcttc
    15901 gtgacttgct ttcttcggtc tttttcgttt tattatcaat ttgtaaacga tttacaattt
    15961 gagtcaaatt atggtaaaat agtgtcatga gtaaaatgta ctatgcagaa aatcctgacg
    16021 ctgctcacga cattcatgag ttgagagtgg acttgttggg agaaaaaatg acttttttga
    16081 cagatgcggg tgtttttagc aagaaaatgg ttgactttgg aagtcaactc ttgctcaagt
    16141 gtctggatgt caaccaagga gagacagtcc tagatgtagg ctgtggttat ggtccattag
    16201 gtttgtcctt ggtcaaggtt tacggagttc aggcgaccat ggttgatatt aacactcgtg
    16261 ccctggattt agctcagaga aatgctgaaa aaaataatgc aaaagcgacg atatttcaat
    16321 ctaatattta tgcaagagtt caagggcgat ttgaccatgt catttccaat cctcctatcc
    16381 gtgcgggcaa gcaagtcgtt catgaaatca tcgagaaaag taaagatttc ttggaaattg
    16441 gtggagattt gaccatcgtc atccagaaaa aacaaggagc tccaagtgcc aaatctaaga
    16501 tggaagatgt gtttggcaat tgtgaaatcc ttaaaaaaga caagggatac tatatcctta
    16561 gaagtgtgaa agaatgagag cagttgattt aatccaaaaa aaacgagacg gtcaagaact
    16621 gacttcaagt gaaattgaat ggctgataga aggctatgta tcaggaactg ttcctgatta
    16681 tcagatgtct gcctttgcta tggctgttta ttttaaagga atgacgactc gagaaatctc
    16741 tgatttaacg atgaatatgg ttaagactgg gcaagagttt gacctgtcag ccattgatgg
    16801 tgtgaaagtt gacaagcatt ctactggtgg tgtcggcgat aaggtaacct tgattttagc
    16861 tcctcttgtt gctagcttcg gtgtgcctgt agcaaaaatg agtggtcgta gtcttggcca
    16921 tacaggcgga acaattgata aattggagtc cattaggggc tatcaagtag aacgtagtca
    16981 agaggatttt attcgtcagg ttcaggatat tggagtatct gtcattgggc aatcagacca
    17041 gctggtcaaa gcagataagc ttctctatgc ccttcgtgat gtgaccgcaa ctgtcgacac
    17101 gattcctttg attgcgagtt cggtgatgag caagaaaatt gcggcagggg cggatgctat
    17161 tttgctagac gtgactgtcg gtgagggtgc cttcatgaag acggttgatg aggcgcgtga
    17221 gttggctcaa actatggtag atcttggtaa ggttgttgga cggaagacag tagcagtgat
    17281 tacggatatg agccagcctt tgggacgagc gattggaaat cgtctggaaa tccttgaagc
    17341 attggagatt ttgcaaggtc aaggacgtca ggatattacc cactttattt gtgaattggc
    17401 tcaaattatg cttggtttgg caaatgttaa caaaactgtt gaagaagttc gccaacatct
    17461 tgaaaatggt caagcactgg ctaagtttga ggaaatggtg caagcccaag gtggagactt
    17521 ggaagacctc tatcgtcctg taaatgtagc tcatgtggtg gaaatccctg ctcaggaaac
    17581 gggtgtcatt tcagctcttc cagctatgga ctttggtctt tatgccatga ggctgggtgc
    17641 tggtcgtgca gttaagtctg atgtcttgga ctatgaaaca ggaattgttt ttgaaaagaa
    17701 gattggagac tccgttcaaa agggtgaaat tgttgcaaaa gtatacacaa atgaaaaaat
    17761 tccacctcaa ctagttacag atttccaaaa atgtgttaaa ataagtgatg aagtgaaaaa
    17821 aatccgtgaa attgtagaaa tcatctccta aatggaggga aaaccgaaat gaaattaaat
    17881 aaatatatag atcatacgct tttaaaacaa gatgcaaaga aaaaacaaat tgatagtttg
    17941 ttgtctgagg ctagagagta tggctttgcc agtgtttgcg ttaatccgac ctgggttgaa
    18001 catgctaaaa aaggacttga aggtacagat gttaaggttt gcacagtagt aggtttcccc
    18061 ttgggagcaa caacttcaac tgtgaaagct tttgaaacaa aagaagctat ccaaaatggg
    18121 gctgatgaga ttgacatggt aatcaatgtt ggggctctca agtcaggtga tttggccttg
    18181 gttgagtcgg atattcgtgc agtagtggaa gcaagtgggg acaagttagt gaaagttatt
    18241 attgaagctt gccttctgac agaccaagaa aaagttttgg cttgccaatt agcccaaaaa
    18301 gcaggagctg actttgtcaa aacatctact ggcttttcaa ctggtggtgc tacgatagca
    18361 gatgttagat taatgcgtga aacagttgga cctgatatgg gtgtcaaggc cgccggtgga
    18421 gctcgttctt atgcagatgc tcttaccttt gtcgaagcag gtgcgacccg tatcggaacg
    18481 tcagctgggg tagctatttt aaaaggagaa ttggcagatg gcgactactg agttgattga
    18541 actggcaatt gaaaccagca aacatgccta tgtcccctat tctcactttc ctatcggagc
    18601 agttttagta gccaaagacg gaagtgttta cacgggagtg aatatcgaaa atgctagcta
    18661 tcctttgacc aattgcggtg agagaacagc tatttttaag gctatatctg aaggccaaag
    18721 agaattttca gaattgattg tctatggtca aactgaaaaa ccgatttcac catgtggtgc
    18781 ttgtcgccaa gtaatggtcg aattttttga acaagattta aaagtgaccc tagtcgcaaa
    18841 agataaatcg acggtcgaga tgacggtcgg ggagttactt ccatactctt ttacagactt
    18901 aaattagtct gagtcgctct ttgagtggca agggtctttg tgaccaatca atccatactt
    18961 gcaacatcgt tgcacatctt atttaggagg ttcagtaatg aacaagaaac aatggctagg
    19021 tcttggccta gttgcagtgg cagcagttgg acttgctgca tgtggtaacc gctcttctcg
    19081 taacgcagct tcatcttctg atgtgaagac aaaagcagca atcgtcactg atactggtgg
    19141 tgttgatgac aaatcattca accaatcagc ttgggaaggt ttgcaagact ggggtaaaga
    19201 acacaatctt tcaaaagata acggtttcac ttacttccaa tcaacaagtg aagctgacta
    19261 cgctaacaac ttgcaacaag cggctggaag ttacaaccta atctttggtg ttggttttgc
    19321 ccttcacaat gcagttgaag aagcagctaa agaacacact gacttgaact atgtcttgat
    19381 tgatgatgtg attaaagacc aaaaaaatgt tgcgagcgta actttcgctg ataatgagtc
    19441 aggttacctt gcaggtgtgg ctgcagcaaa aacaactaag acaaaacaag ttggttttgt
    19501 aggtggtatc gaatctgaag ttatctctcg ttttgaggca ggattcaagg ctggtgttgc
    19561 gtcagtagac ccatctatca aagtacaagt tgactacgct ggttcatttg gtgatgcggc
    19621 taaaggtaaa acaattgcag ccgcacaata cgcagccggt gcagatattg tttaccaagt
    19681 agctggtggt acaggtgcag gtgtctttgc agaggcaaaa tctctcaacg aaagccgtcc
    19741 tgaaaatgaa aaagtttggg ttatcggtgt tgaccgtgac caagaagcag aaggtaaata
    19801 cacttctaaa gatggcaaag aatcaaactt tgttcttgta tctactttga aacaagttgg
    19861 tacaactgta aaagatattt ctaacaaggc agaaaaagga gaattccctg gcggtcaagt
    19921 gatcgtttac tcattgaagg ataaaggggt tgacttggca gtaacaaacc tttcagaaga
    19981 aggtaaaaaa gctgtcgaag atgcaaaagc taaaatcctt gatggaagcg taaaagttcc
    20041 tgaaaaataa tggatggaag tcatttctta ggaagcggcc aagggccgct tttttaagag
    20101 ttgagacaaa gtcattttgt ctcagtaaag cttgctacta gacaaactag caagttttat
    20161 tgaaataaaa taagactctg aaaggaagag cacatggcac acgaaaatgt cattgagatg
    20221 cgtgatatta ccaaggtgtt tggtggattt gttgccaacg acaaaatcaa cttgcaccta
    20281 cgaaaaggtg aaattcatgc acttttagga gaaaatgggg ctggtaagtc cacgctaatg
    20341 aacatgttag caggccttct tgaaccaacc agtggtgaaa tcgcggtcaa cggtcaagtt
    20401 gtcaatctcg actccccatc taaagcagct agcttgggaa tcgggatggt tcaccagcac
    20461 tttatgttgg ttgaagcctt cacagtggct gaaaacatca ttttaggtag tgaattgact
    20521 aaaaatggtg tgctagatat cgctggagct agcaaagaaa tcaaggctct ttctgaacgt
    20581 tatggcttag ctgttgaccc ttctgccaag gtagcagata tctcagttgg agcccaacaa
    20641 cgtgtagaaa ttttaaaaac actttatcgg ggggctgata tccttatctt tgacgaacca
    20701 acggctgttt tgactccatc agaaattgat gagttgatgg ctattatgaa aaatcttgtc
    20761 aaagaaggaa aatcaattat cttgattacc cacaaattgg atgaaattcg cgcagtttct
    20821 gaccgtgtta cagttatccg ttgtgggaaa tcaattgaaa ccgttgaaat tgcaggggct
    20881 accaatgctg atttggcgga aatgatggta ggacgttctg tttcctttaa aacagagaag
    20941 caagcctcta aaccaaaaga agtggttttg tctatcaaag atttggtggt caatgaaaac
    21001 cgtggtgttc cagctgttaa aaatctgtcc ttggatgttc gtgctggaga gattgttggt
    21061 attgcgggga ttgatggaaa tggtcagtct gaactgattc aagccattac aggtcttcgt
    21121 aaggttgaat ctggtagcat tgagctaaaa ggagattcaa ttgtaggctt gcacccacgt
    21181 cagattacag aactaagtgt tgggcacgtt ccagaagacc gtcaccgtga tggcttgatt
    21241 ttggaaatga tgatatctga aaatattgcc cttcaaacct actataaaga accacatagt
    21301 aaaaatggaa ttttgaatta ttcaaatatt acttcttatg ctaaaaagct gatggaagag
    21361 tttgatgttc gcgctgccag tgaattagtt cctgcagctg cactctcagg aggaaatcaa
    21421 caaaaagcaa ttattgctcg tgaaattgat cgagatcctg atctccttat cgttagccag
    21481 ccaactcgtg ggttggatgt cggtgccatt gagtatatcc acaaacgctt gattgaagag
    21541 cgtgataatg gcaaggctgt ccttgttgtc agctttgaat tggatgagat tttaaacgtc
    21601 tcagaccgta ttgccgttat ccacgatggt aagattcaag gtattgtatc accagaaaca
    21661 accaataaac aaggacttgg tgtcttgatg gctggtggaa acttgggaaa ggagaagagt
    21721 gatgtctaaa aaattacaac aaatttcggt tcccttgatt tctgtattcc taggaatttt
    21781 actcggagcc attgtcatgt ggatcttcgg ttatgatgct atttggggct acgaagaatt
    21841 gttctataca gcctttggca gtctgcgtgg gattggagaa atcttccgtg ctatgggtcc
    21901 tctggtcttg attggtcttg gttttgcggt tgccagtcga gctggtttct ttaacgtcgg
    21961 acttcctggt caggctttgg caggttggat tctcagtggt tggtttgccc tgtcgcatcc
    22021 agatatgccc cgtcccttga tgattctagc aaccatcgtg attgccttga ttgctggtgg
    22081 gattgtcgga gcgattccag gtattcttag ggcctatcta gggacgtcag aggttattgt
    22141 aaccatcatg atgaactaca ttgtcttgta tgtagggaat gcctttatcc atgctttccc
    22201 taaagacttc atgcaaagta cagattcgac cattcgtgtt ggggctaatg caacctatca
    22261 gacaccttgg ttagctgagt tgactggtaa ctcacggatg aatattggta ttttctttgc
    22321 catcattgcc gttgcagtta tttggttcat gctcaagaaa acaactcttg gttttgaaat
    22381 ccgtgcagtt ggtcttaatc cacatgcttc agaatatgct ggtatttctg ccaagcggac
    22441 tattattcta tctatgatta tttcaggtgc cttggcaggt cttggtggag ctgttgaagg
    22501 tttgggaacc ttccagaacg tctatgttca aggttcgtca ttagctatcg gatttaacgg
    22561 aatggcggtt agtttgcttg cggccaactc accaattggt atactctttg cagccttcct
    22621 atttggcgtt ctccaagttg gggctcctgg tatgaatgcg gcgcaggtac catctgagct
    22681 tgtcagcatt gtaacagcgt ctattatctt ctttgtcagt gttcattacc ttatcgaacg
    22741 ctttgtcaaa ccgaaaaaac aagttaaagg aggtaagtaa agatgtctat tataaccttg
    22801 ctccccctct tggtgtcttc tatgctgatt tactcagcac ctctcatctt tacaagtatc
    22861 ggtggtgttt tctctgaacg tggtggtgtg gtaaacgttg gccttgaagg aattatggtt
    22921 atgggtgcct tttctggagt tgtctttaac cttgaatttg cagaacaatt tggagcagca
    22981 actccatggc tatccttgct tgtagcagga ttggttggta gtgttttctc tatcatccac
    23041 gcagcagcga cggttcattt ccgtgcagac catgttgtca gcggtacggt attgaacttg
    23101 atggcgcctg ccttggctgt tttcttagtt aaagttcttt ataacaaagg acaaaccgac
    23161 aacctaagtc aaacttttgg acgctttgat ttcccagtct tggcaaatat cccagtgatt
    23221 ggtgatatct tcttcaagtc aactagtcta cttggttatc tggcgattgc cttctcattc
    23281 cttgcttggt ttattctctt taagactcaa tttggtcttc gtctccgctc tgtcggtgaa
    23341 catcctcaag cagcggatac cttgggaatc aacgtctaca agatgagata tttaggggtt
    23401 attatttcag gttttctagg tggaattggc ggagcgattt atgcgcaatc aatctcagtt
    23461 aacttctcag tgacaactat tgttggacct ggatttatcg cccttgctgc gatgatcttt
    23521 gggaaatgga atccaatcgg tgctatgctt tctagtctct tctttggact ttcacaaagt
    23581 ttggctgtta tcggttctca attgccgttc ctacaaggag tgccagcggt ttatcttcaa
    23641 attgcacctt atgttttgac aattcttgtc ttagcagcct tctttggaaa agcagtcgca
    23701 ccaaaagcag atggtatcaa ctacatcaaa tcaaaataag catacaaaaa aacgtcagtc
    23761 caatgactga actgc
//
LOCUS       DAVKWS010000026        23612 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKWS010000026 DAVKWS010000000
VERSION     DAVKWS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.026"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            4..1224
                     /locus_tag="VW852_001161"
     CDS             4..1224
                     /locus_tag="VW852_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV0272318.1"
                     /translation="MLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILYEKDATQP
                     VEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVE
                     ELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTTGLNNETL
                     GDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFARMTITSTNYML
                     EGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATS
                     SLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERVGNQSSQS
                     VQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKIEKAFFLK
                     VWWNQFVRFVNEKL"
     gene            complement(1455..2867)
                     /gene="sufB"
                     /locus_tag="VW852_001162"
     CDS             complement(1455..2867)
                     /gene="sufB"
                     /locus_tag="VW852_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV0272319.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2921..3361)
                     /locus_tag="VW852_001163"
     CDS             complement(2921..3361)
                     /locus_tag="VW852_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV0272320.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAGQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDVVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3348..4574)
                     /locus_tag="VW852_001164"
     CDS             complement(3348..4574)
                     /locus_tag="VW852_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0272321.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPALARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4585..5847)
                     /gene="sufD"
                     /locus_tag="VW852_001165"
     CDS             complement(4585..5847)
                     /gene="sufD"
                     /locus_tag="VW852_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV0272322.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5875..6645)
                     /gene="sufC"
                     /locus_tag="VW852_001166"
     CDS             complement(5875..6645)
                     /gene="sufC"
                     /locus_tag="VW852_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV0272323.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6795..6989)
                     /locus_tag="VW852_001167"
     CDS             complement(6795..6989)
                     /locus_tag="VW852_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV0272324.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7017..8672)
                     /gene="dnaX"
                     /locus_tag="VW852_001168"
     CDS             complement(7017..8672)
                     /gene="dnaX"
                     /locus_tag="VW852_001168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV0272325.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PALSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLAGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8672..9169)
                     /locus_tag="VW852_001169"
     CDS             complement(8672..9169)
                     /locus_tag="VW852_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV0272326.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            9362..10679
                     /locus_tag="VW852_001170"
                     /pseudo
     CDS             9362..10679
                     /locus_tag="VW852_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10763..11965)
                     /gene="rpsA"
                     /locus_tag="VW852_001171"
     CDS             complement(10763..11965)
                     /gene="rpsA"
                     /locus_tag="VW852_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV0272327.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(12127..12198)
                     /locus_tag="VW852_001172"
     tRNA            complement(12127..12198)
                     /locus_tag="VW852_001172"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12164..12166),aa:Gln,seq:ttg)
     gene            complement(12208..12288)
                     /locus_tag="VW852_001173"
     tRNA            complement(12208..12288)
                     /locus_tag="VW852_001173"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12252..12254),aa:Tyr,seq:gta)
     gene            complement(12338..12568)
                     /locus_tag="VW852_001174"
     CDS             complement(12338..12568)
                     /locus_tag="VW852_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV0272328.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(12736..13380)
                     /gene="pcp"
                     /locus_tag="VW852_001175"
     CDS             complement(12736..13380)
                     /gene="pcp"
                     /locus_tag="VW852_001175"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV0272329.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(13395..14318)
                     /locus_tag="VW852_001176"
     CDS             complement(13395..14318)
                     /locus_tag="VW852_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV0272330.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(14315..15004)
                     /locus_tag="VW852_001177"
     CDS             complement(14315..15004)
                     /locus_tag="VW852_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV0272331.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(15069..15773)
                     /locus_tag="VW852_001178"
     CDS             complement(15069..15773)
                     /locus_tag="VW852_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0272332.1"
                     /translation="MVLSTLAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(15829..16851)
                     /locus_tag="VW852_001179"
     CDS             complement(15829..16851)
                     /locus_tag="VW852_001179"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV0272333.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(16983..19454)
                     /gene="parC"
                     /locus_tag="VW852_001180"
     CDS             complement(16983..19454)
                     /gene="parC"
                     /locus_tag="VW852_001180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV0272334.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(19874..21817)
                     /gene="parE"
                     /locus_tag="VW852_001181"
     CDS             complement(19874..21817)
                     /gene="parE"
                     /locus_tag="VW852_001181"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV0272335.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVMTLK
                     KVGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            21953..22594
                     /gene="plsY"
                     /locus_tag="VW852_001182"
     CDS             21953..22594
                     /gene="plsY"
                     /locus_tag="VW852_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV0272336.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(22637..23612)
                     /locus_tag="VW852_001183"
                     /pseudo
     CDS             complement(22637..23612)
                     /locus_tag="VW852_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         6836 a         5276 c         4264 g         7236 t
ORIGIN      
        1 actttgttaa ctgtctctct tttagggggt gcttctactg ctgttgctca agattttacc
       61 attgccgcta aacatgcgat tgctgttgag gcaaatactg gtaaaattct ctatgagaag
      121 gatgcaacgc aacctgtcga aattgcttcc ataacaaaat tgattactgt ttatctggtc
      181 tatgaagctt tggaaaacgg aagtattacc ctctccactc ctgtagatat ttctgattat
      241 ccttatcaat tgacgacaaa ttctgaagcc agtaatattc ctatggaggc ccgtaattat
      301 actgtcgaag agttgcttga agcaactctg gtatctagtg ccaacagcgc cgctattgcc
      361 ctagctgaga aaattgctgg ctcagaaaaa gatttcgtcg atatgatgcg ggcaaaactc
      421 ttggaatggg gaattcagga tgccactgtt gtcaatacga caggtcttaa caatgaaact
      481 ctaggggata acatttaccc aggttctaaa aaagatgagg aaaataagct tagtgcttat
      541 gatgtcgcta tcgttgctcg caacctcatc aaaaaatacc cacaagtctt agaaatcacc
      601 aaaaaacctt cttctacttt tgctaggatg acaatcactt caaccaacta catgttagaa
      661 ggtatgcctg cttaccgtgg tggttttgat gggctaaaaa caggaacaac agataaggct
      721 ggagagtctt ttgttggtac tactgtcgaa aaaggcatga gagtcatcac agttgtttta
      781 aatgcagatc atcaagacaa taatccttac gctcgattta cagctacatc ttccctaatg
      841 gattatattt cttctacatt tacacttcgc aaaatcgttc aacaaggcga tgcctatcaa
      901 gatagcaaag cccctgtaca agatggaaaa gaagatacag taactgcagt ggctccagag
      961 gatatctatc taatcgaacg tgttgggaat caatcttccc aatctgttca attcacacct
     1021 gattccaaag caatcccagc accacttgaa gctggaacag tggttggcca tttgacttat
     1081 gaagacaagg acttgattgg tcaaggttac atcaccacag agcgccctag tttcgaaatg
     1141 gtagcagaca agaaaattga aaaagccttc ttcttaaaag tttggtggaa tcagtttgtc
     1201 cgctttgtta acgagaaatt ataaaaaatc gcgattttaa accgcgattt tttgatacaa
     1261 gttcatcttc aaaatccttg attttgagta aatctaatac tcaaaatcaa agaacaaact
     1321 aggaagctag cctcaggttg ctcaaaacac tgttttgagg ttgtagatga aactgacgaa
     1381 gtcagtaacc atacatacgg taaggcgatg ctgacgtggt ttgaagagat tttcgaagag
     1441 tataaaatca aattttatcc aactgatccc tccatttcat agctaatcaa gcggttcagc
     1501 tcaactgcgt attccattgg aagttctttt gtaaagggtt ctacaaatcc catgacaatc
     1561 atttcagttg cctcagattc tgacaatcca cggctcatga gataatacaa ttgctcttct
     1621 gaaatcttag atactttggc ttcgtgttcc aaagccactt gcgagttgtg aatttcatta
     1681 aatggaatag tatctgatgc tgacaagtca tccatgataa tggtatcaca ttcaatgtgg
     1741 gaaacagatt tcttagagtt cttgttaaag gtgacttgtc cacggtagtc aacctttcct
     1801 ccacctttag cgatggattt agacacaata gacgagctgg tatgtggagc attgtgaatc
     1861 atcttagcac ccgtgtcttg gtgttgccct gcattagcaa aggcgataga gagcatggta
     1921 ccacgcgctc cttctccatc aaggtaaaca gatggatatt tcatagtcgt tttggcaccc
     1981 aagtttccat caatccactc aacagtggca tccttttgag ccttagcacg ctttgttacc
     2041 aagttataga cgttatcaga ccagttttgg atagttgtat aacgcatata agctccgtcc
     2101 aaagcaaaaa tttctacaat ggcagcgtgt aagctattgc ttgaatatgt tggtgctgta
     2161 catccttcta cgtagtggac gcttgctccc tcatcaacga taatcaaggt acgttcgaac
     2221 tgacctatat tttcgttatt gatacggaaa taagtttgaa gtggaatatc taccttgaca
     2281 ccttttggca cgtagataaa agttccaccc gaccatactg ctgagttgag ggctgccaac
     2341 ttgttatctg tcggcggtac caacttcgca aagtattgtt taaataagtc tgggtattcc
     2401 ttgagtgcgg aatctgtatc tgtaaagata atacctaatt tttggaactc ttccttcatg
     2461 ttgtggtaaa ccacttctga ctcgtactgg gcagaagccc ctgctaaata agcacgttca
     2521 gcttctggaa tcccgatacg ttcaaaggtt tctttaatct tttcaggtac atcatcccaa
     2581 gaacgggctg gtttgtcaga tggtttttgg tagtagatta agtcatcaaa gtcaatctct
     2641 gacaagtctg ctccccaagt ttgcatgggc atttttttga aggtttcata agacttcaaa
     2701 cggaactcca acatccactc aggctcaccc ttagcagcag ataattcacg aataacacct
     2761 tcgttgagtc cttttcctgt cgataagaca ggctctacat cgtcatggaa accaaattta
     2821 tattcaccaa ggtcaattgg ttttggttct actctttctt cagccatagt atcctttctt
     2881 tcttcatttc taataattca taagacaaaa gaaacttgtc ttactgtttt tcttgatttt
     2941 caattgtttt cttaagggca ttccaagcta gggttgcaca cttgattctt tgagggaatt
     3001 tggcaacacc tgacaagaat gccgcgtctc caagttggcc ttgacgctca tctttttgcc
     3061 cttgaaccat ttcagaaaaa atagtcgcca gttctaaaat ttcttgtttg gtttttccta
     3121 aaacgacatc tgtcatcata ctagcagaag cagttgaaat cgtgcatcct gaatttagaa
     3181 aagcaatatc ttccaaacgg tcctctgcat caaacttgac agagaggttg atgacatccc
     3241 cacaagtcgg attgttgaga ctgatttgcc cagcatcttc taacttccct tggtgatgtg
     3301 gatttttcga atggtctgct accactgcca tataaaggct atctagttta gaaagtgcca
     3361 ttgaaaaact cctttgtctt ttgtagggca tcgactagtt tgtcgcaatc tgccttggta
     3421 ttgtagatat aaaaacttgc acgagctaat gctgggactt ccaaatactg aagcaagggt
     3481 tgcgcacaat ggtgaccagc acgaacagcc actccttcat aatccagagc cgtcgcaaga
     3541 tcgtgaggat ggagatcacc taggttaaag gcaataacac ccgaacgttg agccaaatcc
     3601 tgagaaccgt aaatggtcaa tccctcaatt gcctgcagtt ttggatagac gtacgcaatc
     3661 aattcctgtt catgagcttc aacggcatcc ataccaatct tttccagata atcaactgca
     3721 gtcgcaagtc caatagctcc tgccatattt ggcgttccag cctcaaattt ccaaggcaat
     3781 tccttccaac tagcggattg ctcgtagaca aaatcaatca tctcgccgcc aaattctact
     3841 ggagacattt gctcaagata cttttctttg ccgtaaagga caccgatacc agtcggacca
     3901 gccatcttgt gacccgaaaa ggcgaaaaag tccagatcca agtcctggac atcaatcttc
     3961 atatgaggtg tagattgagc accatccact accatgattg ccccaacttg gtgggctaat
     4021 tgagtgattt ccttgatcgg attgaccaca ccaagaacat tggaggcatg agctagggaa
     4081 acaaatttaa ccttatcagt caatttagct cgcaaatcct ccatatccaa ggcaccgtct
     4141 ttaagataga catagacaag ctctgctcca gtctttcgac aagcttcctg ccatggaatg
     4201 atattagaat ggtgttccat tactgaaatc aagacctggt ctccctcagt gagaatttcc
     4261 tcagcaaagc gtgccaccca gttaaggctg gttgtcgttc ctctggtaaa gagaacttcc
     4321 tttgtagagc ctgcattaat aaacttacga atggtttcac gagcagcttc ataagaagct
     4381 gtcgctcgtt ccgctaaggt atggacacca cggtgaacat tggcattgtc ctgctcatag
     4441 taactgttaa tagctttcag aactactagt ggtttttgtg tcgtcgcagc attgtccaga
     4501 tagaccagag gttcatcatt gacaatctga tctaaaattg gaaaatcctt gcgaatcgct
     4561 tctacatcta acataggctt ccccttagcg ttttgacaat ttctcttcga tagttgcaat
     4621 catttcatca cgaacttcct tgactggaat ctccacgata acagatccaa ggaaaccacg
     4681 aacaaccaaa cgctctgcag ttgccttatc caatccacga ctcatgaggt aatacatgtc
     4741 ttctggatca acttgtccga tagacgctgc gtgtcctgca gtgacatcat tttcatcaat
     4801 caaaagaatt gggttagcat ctgaacgcgc ttggtctgaa agcatgagaa cacggctctc
     4861 ttgttgcgca tctgctccct tagcaccctt gatgatgtgg ccgataccat tgaaagtcaa
     4921 agttgctttt tcaaggataa ccccatgttg taggatattt ccgatagagt tgcagccata
     4981 gttagttaca cgagtatcaa tcccttgtac ctgacgacca cttgaaagag ctacaacctt
     5041 gaggtcagca tggctaccat taccaatcaa gtcactatca aaatcagcaa cgacatttcc
     5101 ttcgttcatg acaccgatag cccagtcaat acttgcatcg ttgcctaatt taccacgacg
     5161 gctaatgtag gcagtgacgt tttcacctag acggtcgata gcagcaaact tgacttgcgc
     5221 accagaacgt gcaatcactt ccactgtgat attggcagtt actttgtcac ttccttcacc
     5281 gcgtgactct aaacgctcca gataactaat cttagaattt ttaccagcga taatcataat
     5341 atgcttgtta aacggcacat tgctatcgct atcttggtag aaaattcctt caattggctc
     5401 tgtgatttct acgttatctg gaatatagag tacagcacca ctgttaaagt aagctgtgtg
     5461 gtaagccgcc aacttgtcat catcatactt aacagatgac atgaagaatt cttcgatcag
     5521 ctctggaatt tcttctaaag ctgagtgaaa gtctgtgaag acaacaccct gttcagctaa
     5581 ctcaactgga gtttgttcga aaacagtttg agttcctact tgcaccaact tcaagtgatg
     5641 atctaaagct gtgaaatctg gaacatttgc tgatggctca ttttctgtaa tcgttccatc
     5701 acccagattc caacggtgga atttgacacg ctcaataact ggtaattcca aagtctcaat
     5761 cttgtcaaaa gctttttgac ggagatcagc caaccagctt ggttcagcgt gcatttctga
     5821 aaaaagttta atattttctc tagtcattta cttctcctaa aagatacgag ggaattacaa
     5881 ttcttccttg tagtcgtagc caagttcttc agctaatttt gcgtatcctt cacgttccaa
     5941 acgcgcagcc aattctggac caccagaaag gacaacacga ccttccatca tcacgtgtac
     6001 cacatcaggt gtgatatagt tcaaaagacg ttggtagtga gtaatgatca tagcaccaaa
     6061 accttcgcca cgcatggcat tgacaccttt agacacaact ttaagagcgt caatatcaag
     6121 acctgagtca atctcgtcca aaagagcaaa tgttggctcc aacatcaaaa gttgaagaat
     6181 ttcattgcgt tttttctcac caccagagaa gccttcgttg aggtaacgct ctgccatttc
     6241 ttctttcatg ttgagcaatt ccattttttc atctagctta gtaataaact cacgaactga
     6301 aatcttctca tcatcttctt tacccgcatt catagcggca cgaagaaact cagcattggt
     6361 aattccaggg atttctgatg ggtattgcat agcaaggaaa agtcccatac gcgcacgctc
     6421 atccacttcc aactcaagga tgtttacgcc atcaaacaaa acttcacctt tagttacttc
     6481 atagtttgga tttcccatga tagcggcaga aagagtcgat ttacctgtac catttggtcc
     6541 catgatagcg gcaatttctc ctgttttcag ggtcaggtta acccctttta aaatttcttt
     6601 tccttcaatc tcaacgtgaa gatctttgat ctctattact gacatgatag ttcctttctt
     6661 tttcaaaact tttacagtac ttactagaaa aacgtctgat ttccccagaa atagagcaaa
     6721 aattctataa atatactttt ctagtataac aaaaaaaagc ctaaaaggct ttgattttgt
     6781 ctggaagctg agggctagtc tttcctatta aaatgctgat cgatgacatc aacgattttg
     6841 cccatcacat aactgactcg gaaatttcta aagagtaaaa ttgcccaaaa ggctgccata
     6901 atatagcggc cagtcatcca ggcttcattg aaaaaatagg tctcagcagc tgtaaatagc
     6961 gccataataa taaattgttg tttattcata aacttattgt atcatgaaat ctttctttag
     7021 tcttcctcta ccttcacttt atcagccaaa aattcaaatc cttctggaat caggctttct
     7081 tctacttctt tttcagtttg agaagatttg gctttggctg aaaaggctgc gcgaacttct
     7141 ttccattcct ccatggaaat agctaaaatc tcaggtgaaa aacctgccgc ctgactgagg
     7201 atattaccaa acatggtatt gagattgtct cgtttcatag tttgaccagc attgaagtta
     7261 gactcaaaag caagaatagc atggtgttca ttggcagcaa ccggttgaga accagctagc
     7321 agagccttgt ccggcccacc tagactttca attacctctc cccaggcatt ctgcaaacga
     7381 attaaatttt gacgtgctaa atcaggattt tcgacggcct cttgtaagat agattgcact
     7441 ttattgcgat cgacacgata gactgttttg cccgtagctg gtcgactaag agctggtgca
     7501 acttgtttag gaaccgcacc tacattagaa agctcttgtt tgagacgggc aacttcctgt
     7561 ctcagcgtag caatttcatt ttcaaccgct cctgatagag ctggttcggg cttgatttcc
     7621 gccaaacgga cggtcatcat ttcagcataa atcttgggct gcaaactaga cttaatatct
     7681 gctaaactca ctgttgctaa gcgaatcatt tcaaacagat ttttttgagg aagtgccaaa
     7741 ttttctacaa agactgaact atgatgagta ttttctcccc ctgtttgaac aattaacaag
     7801 tctcttaaat agtgcaaaag atcggtcaca aaacgagtca tgctcttacc attgtcaaaa
     7861 agaagattca agcaagacaa agctttggga acatcctgtt gagacaaggc cgccacataa
     7921 tcatccaagg ctgataggct aatggtgcca gtaatttctt cagagatagc agtcgtcagc
     7981 tcatttccct gtgtcaaact cagggcttga tccaaaatag acaaggcgtc ccgcattcca
     8041 ccttccgccc gtctggcaat gatttccaca gcctctggtt cagaactgat attttctttt
     8101 tctaagatat agtgaatatg ttccttaata tcctgtgtct taattgattt aaactcaaaa
     8161 cgttgcacac gggatagaat agtagcagga atcttgtgca attcagtagt ggccaaaata
     8221 aagactacat tctgtgttgg ttcttccagc gtctttagga gggcattaaa agcccctgta
     8281 gacagcatgt gaacctcatc tatgatataa accttataac gagcaaggct aggtgcatag
     8341 gtagatttat cacgaatttc gcgaatttca tctaccccat tattagaagc tgcatccatt
     8401 tcaatgacat cttctaaact accgtccgtc actgcttgac aaatatagca gttattgcaa
     8461 ggttcgccac ccacttgatt gggacagttc atagccttgg caaagatttt agcaacactg
     8521 gtttttcccg ttccacgagg accagaaaaa agataagcgt gacttatttt ctcttgctcc
     8581 accgcttgtt taagagtctt agccacaact tcttgaccaa ctaactggga gaagttttga
     8641 cttctatatt ttcgataaag tgcttgatac attaagattt ttcctcaaac atcgtaaagt
     8701 cccatgttgt cttttcaagc aaaatagcga caaattgttc caaataatct cgatccatag
     8761 catcgtaatc ctcaatctct gaagaatcca gatccagaac tccaagtaac tgaccattct
     8821 tcatcatcgg caccacaatt tcacttttag ctagactatc acaagaaata tagttgagat
     8881 aggtcgtcac atctccaaca ataacagttt cctgaaagtg agctgcctca ccacaaacac
     8941 ccttgcctag tgcaatacgg atgcaggaaa cacctccttg gaaggggcct aaaaccaatt
     9001 cctttccatc gaacaaataa aagcctgcaa atacggtatt aggaaaacgt gattttatga
     9061 gagcactggc gttggaaaga ttagccaaaa cattggtttc gccttccaat aggaaggaca
     9121 attcttcatt taacatttga taacgtgatt gtttttctga ttttaacata gaactattat
     9181 atcaaaaaag gagcagagac aaaagaaaaa tcccttgttt agtaaacaaa ggattttggg
     9241 ctctataata tttgtagtgg gtaaatcccc tatggatatt atggagccta ttttgtgtag
     9301 aaaaaaagtc ccatatgacc tataatgaaa agcgaccaaa caactcatta gaaagattca
     9361 tatggaacaa ttacatttta tcacaaaact gctcgatatt aaagacccaa acatcaagat
     9421 tctagatatc atcaatatgg atacccacaa agaaattatc gctaagctgg attatgaggc
     9481 tccatcttgc cctgattgtg gaagtctaat gaagaaatat gactttcaaa aaccgtctaa
     9541 gatcccttac ctcgaaacaa ctggtatgcc ttctagaatt ctccttagaa aacgccgttt
     9601 caagtgctat cactgttcaa aaatgatggt cgctgaaact tctatcgtca agaagaatca
     9661 tcaaattcct cgtattatca accaaaaaat tgcgcaaaag ttgattgaga agatttctat
     9721 gaccgatatt tctcatcagc tggccatttc aacttcaact gtcattcgca agctcaatga
     9781 ttctcacttt gagcatgatt tttcgcgtct tcctgagatt atgtcctggg acgttgaaac
     9841 agtccgggga gtgactgttt caatcgggag atggagatga gctttattgc gcaagatttt
     9901 aacaatctca atatcatcac tgttcttgag ggtagaacac aagctatcat tcgaaatcac
     9961 tttcttcgct acgatagagt tgtccgatgt cgcgtcaaaa ttattactat ggatatgttt
    10021 agtccttact atgatttggc taaacagctt cgctttcaaa tttctaggct caggctgaaa
    10081 cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tcttgatcgc tttcacattg
    10141 tacaacatct tagccgtgct atgagtcgtg tgcgtgtcca aatcatgaat caattggatc
    10201 gaaaatccca tgaatacaag gctatcaagc gctactggaa actcattcaa caggatagcc
    10261 gtaaactgag tgataagcga ttttatcgtc ctacttttcg tatgcactta accaataaag
    10321 agattttaga caagcttttg agctattcac aaaacttgaa acaccactat cagctcttgc
    10381 tttttcactt ccagaataag gaaccagaga aacttttcgg acttattgag gacaatctga
    10441 agcaagttca tcctattttt cagactgtct ttaaaacctt cttcaaagat aaggaaaaga
    10501 ttatcaacgc ccttcaacta cactattcta atgccaaact ggaagcgacc aataatctca
    10561 tcaaacttat caagcgcaat gcctttggtt ttcgaaactt tgaaaatttc aaaaaacgga
    10621 tttttatcgc tttgaacatc aaaaaagaaa ggacgaaatt tgtcctttct cgagcttagc
    10681 ttttttcaac ccactacagt tgacaaagag ccgaaaaatc ccttgtttag taaacaaagg
    10741 attttgtgaa ttttcaattt gattaaagtt cgatatcacc aaacaaatca gccattgaaa
    10801 atcctgtttg tgtttctgga agttcgaaat cacgcttttc ttgacgtctt ggacgacgtg
    10861 gacgagcagc acgtttttct tctttttgtc cttcttcttg ggctggacgt tcttcaagag
    10921 ctttaataga aagtgatacg cgttctgcat ctgcgttaac ttcaagaact ttaacttgaa
    10981 cttcttgacc aactttaaga gcttcttttg gattttcaat ccgtttgtgt gaaatttgtg
    11041 atacgtgaac aagtccatcg atacctggca atacttcaac aaatgcaccg aagtcagtca
    11101 aacgtttaac tgttccttct actacatcac ctttagccaa tttttgctca acgccatccc
    11161 atggtcctgg tactgttgct ttaagtgaaa gtgatacacg tccttcttct tcgttaagat
    11221 caaggatttt cacttcaatt tcttcaccaa cagttacaac tgattttggt gatacgttac
    11281 gttcatgtga caattcagtc aagtgaacca atccgtcaac accaccaagg tcgatgaaag
    11341 cgccgaagct tgtgatacga gcaactttac cagttacaac atcaccaaca gccaatttac
    11401 cgaatacttc agcgcgagct gctgcagtag ctgcttcaac aacttcacga cgtgaaagga
    11461 tgaagcggtt ttctttagcg ttaacttctt tgattttagt atcaaattct tgacctacaa
    11521 aacgctcagc gttacgtacg aaacgagtat ccaacattga agctgggata aatccacgaa
    11581 caccttcaaa ttctactgaa agtccacctt taacggcacg cgttccttta acagtaacaa
    11641 cttcttcttc gcgaccaaca agtttgtccc atgctttgcg agcttcaagg cgttttttag
    11701 atacaaggta tgtaactgta tcagtatctt taccaactac ttgacgaagt acaagaacat
    11761 ccaatacttc tcctacttta acaaagtcat tgatatctgc atcacgatcg tttgtcaatt
    11821 cgcgaagagt caagacacct tcaacaccag ttccagagat tgcaacgtta gcttgagtcg
    11881 catcaactgt caatacttca gcactaacaa catcaccagt ctcaacttga ctaacgctat
    11941 ttagcaaatc ttcaaattcg ttcatctaaa aaatcctcca acaatcaagt ttttcttaaa
    12001 cttgacatac ttataatttt tttcctaagc acccgcaagg acatgttcat atcattcacg
    12061 tctttcaatt ataaaaataa aataccctaa gatattttgc tttttatctt gattattacc
    12121 taagaactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta
    12181 ccgcttggct ataccccatt aatgaaatgg agagagaggg attcgaaccc ccgaacccga
    12241 aggagcggat ttacagtccg ccgcgtttag cctcttcgct atctctccta caatcaacat
    12301 ggactattat atcatgaaaa tttcaaaaaa acaagactta ttttgctaaa ttataatttt
    12361 caatcaaaat ttccacagct ttttccaatt ttttataaaa actatcgaca ttcccattct
    12421 ttataatggc aaattgtccg tctaattcgg caatttctcc gataactttt ttaccgatag
    12481 tcaatgtata accttcaaaa ctgtctttac caacattaac tttggcatcc gctacttgaa
    12541 tttcgatttt tttatctttc ttgctcattt catacctctc ttcacttttt ctattttaca
    12601 agaaaatcct aaaactagca agaaaaaact ctatatcagg cttctctgat atagagcttt
    12661 atgtaaacaa cctcaattga tttagcatag ttaatagtta gtaaacaagc ataattgact
    12721 atccttttta ggttatcagt gagtctcacc gcctactaac tgcaaatcct tatctttgta
    12781 gtctacaata gcttcaatag ctacctctat gcctcttaca atgtcacata gactcattcc
    12841 tgcagtattc ggcttattta ctacttgttc catcatatat ggaatatgca tgaaacctgc
    12901 tctcatatta gggaattttt tatccactaa ataaagagct tggtacatca aatgattgca
    12961 aacaaaggtt cctgcactat tggatacaac tgccggaagt ccctgttttt tgatagcttg
    13021 taccatcgct ttgataggta aactactaaa ataggccgat gctccatcaa tacgaatcgg
    13081 tgtatcaatt ggttgattgc cttcgttatc aggtatgcga gcatcatctt gattaatagc
    13141 cactcgttca ggtgttaagc cggtcctgcc gcctgcttgt ccaatacaaa gtacagcatc
    13201 tggttgatat cgtaatattt ctgcctctaa aacttctgac gacttataaa aaaccgttgg
    13261 aatttctacc cagcgaactt cagccccatt aatctcagat ggtaataatt ttacagcctc
    13321 caaagctgga ttaatctttt cacctccaaa aggattaaaa cctgtaacca atattttcat
    13381 tttattttcc tttactaaaa tgcgagaaag tacattaaga atatgtgaat aacaatcatt
    13441 actagagcaa cacctgcttg agcctttata acgccattct gatctttcat atccatcaat
    13501 gctgctggta gagcgttaaa attagcagcc attggggtca ataaggtccc acaataacct
    13561 gctgtcatgg caagagcacc agccacaatt ggattagctc ccagagcaaa tacaaaggga
    13621 actccaacac ctgctgtaat aacggtgaat gctgcaaaag catttcccat aatcattgtg
    13681 aatagaacca ttccaagaac ataggccaaa actcctataa agcgactatc tgaaggaaca
    13741 ataccgctaa tcagatgaga aataacatca ccaacacctg ctacagtaaa aatagccccc
    13801 aaagccccta ataattgagg aacaatccca cttgttgaaa cttgctgagt cattcgatta
    13861 ttttctgata acagactctt agggtgacta ttggtaatca caagaacaga aattgtagca
    13921 aacaaggcgg caaggctaat cgaaatcttg ctaaattctg gaatcatttg cgctaagacc
    13981 aacgcaagta ttgccatcag cataactgga ataaaaattt tatttttcaa cctgttagat
    14041 tcaatattgg ctttcatttc atctaaggat ggcaaggttc cgatacggac ttgcttaaac
    14101 aatgttaaca gcgataatag gattacaata ataccaatac tcatatttgg catataggaa
    14161 ccacctataa acgtaataga caatagagtc caaaatgcag atgtcccaag tcgaactggg
    14221 tttgttttat ctttataact acaataggct gtatggagaa attgacaacc aatcacaata
    14281 taggtcaact ctaatagttg ctttgccaac tctgtcattt ttgttctcct cccctagtct
    14341 tttttgatat caatttttta tcaaataaat aattgtaaat ccccactaca ataagtgtta
    14401 taacagcaac aataatagat gtagaagcaa tccctgcata attgctttca tagcctaact
    14461 gatctaatgt tccccctatc aagaggactc ccccagcacc tacaaacgta ttttgagcaa
    14521 agaaatttcc aaaattttca ttcgcagccg cacgcgcttt tattgtctca tcttcaacct
    14581 ctgttaactt tctacctaat tgagactctg cagctgcttc tcccataggt tgaaccaaag
    14641 gtctgacaaa ctgagggtgt cctcctagac gaattgaaaa gaaaccagct aactctcgaa
    14701 taaagaaata aactgtatag aagtttccaa ctgtcagacc tttaatcttt cgaatcaaat
    14761 cgattgatcg ttgcttgagt ccaaaggttt ctgacagccc cacaagaggc aaggtaacca
    14821 taaaaatcgt gagcactcgc tgattgctaa attcttttcc caaaatctcc aaaaattcaa
    14881 cgagagaaac acctgaaact aaagctgtaa ccaaaccagc taagactact gttgcaattg
    14941 tatcaaattt taaaataaaa cccacaacaa tgattgctat tcctattaat ctaatccact
    15001 ccatatcaaa ctcctttata ttcaaaatga cagtatttta aaaattttat caagatcaat
    15061 accattcctt atttaatgta tttttctagt tctttttggt atttgctatt ggattccaat
    15121 ttttcttttt gccatttttt aaaaacctcg ttatattctt ttgttgtaac aatatctttt
    15181 tgcaatttca ttcctttaaa gatatatggt tcccccttaa taccaacttg tgagtatggt
    15241 tttgagaatg gtactacgtt acttacaact ggagaaccac cagatgaagc tgttggcatc
    15301 aataatgaac tatctgtcga ccaagcttga gctttggcat atttttcata tctcttctct
    15361 aggtcagtgg tctcagaaac agcatcttct aacaatttct tatatttatc caaaccaggt
    15421 ttagctacaa catccttatc ttttcctttc gtaataccaa ggtgtttcat ggcagaacca
    15481 gattttggat ccataatatt caagtaagac gctggatctt gatagcttgg agcccatcct
    15541 gtactattca aatcatagtc tttttgagaa ggagcaacat tgccgtattt atcattttcc
    15601 atcaaaccat caataacatt tccaataacg tctgtcgtcg atgttcgagt cgctatactg
    15661 tagcccaatg atgctggatc tactgcatag acataagaaa atgttgtcgg tgcatctgct
    15721 tctttatcag tttttccaca agccactaaa atagctaacg tgctcaggac cactcctgct
    15781 gttaagagcc actttttcta tttcataaaa aatctccttt ggtttatttt aatctacttt
    15841 tacaatccaa ccttctggcg cttcaatatc gccaaactga atacccgtca attcattata
    15901 taatttacgc gtcacaggac ctacttctgt ttcactatag aatacatgga aatcatcacc
    15961 atgttgaata cctccaattg gagaaataac cgctgctgta ccacaggcac ctgcctctac
    16021 aaaacggtca agattatcaa ttggaacatc accctcaata ggagttaatc ccaagcgatg
    16081 ttctgccaaa taaagcaagg aatacttggt aatagatggc aagatagatg gactcaatgg
    16141 tgttacaaat tcattatcag ctgtaattcc aaagaagtta gctgatccga cttcttcaat
    16201 ctttgtatga gttgatgggt ccagatagat aacatctgag aaatggcgtg acttggccat
    16261 ttttcctggt aagagacttg cagcatagtt tccaccaacc ttagccgcac ctgtaccatt
    16321 tggtgctgca cggtcgtact catcctgaat caagaagttg gttgggacca aaccaccttt
    16381 aaagtaattt ccaactggca tagcaaagat ggtgaaaatg tactcttctg ccggttttac
    16441 cccgataata tctccgacac caatcaaaag agggcgaaga tataaggttc cacctgttcc
    16501 gtatggtggt acgtattctt cattcgcacg gacaactgct ttacaagctt ctacaaacat
    16561 gtctgtcgga acttgtggca tcaagagacg gtcacatgta cgttgcaggc gtttagcatt
    16621 ttcatcagga cggaacagtt gaacactgcc atccttagta cgataagctt tcaaaccttc
    16681 aaatgcttgt tgtccatagt gaagacttgg agaagactct gaaatatgca aagttgcatc
    16741 ctctgtaagc tctccttgat cccattgtcc atttttgaaa tgagcaagat agcgataagg
    16801 taatttcata taggaaaaac cgaggttttc ccaatcaatc gttactgtca tattccactc
    16861 cttattctaa aaacctattt cttatattct acactatttt tctaaaatag caagtatatt
    16921 ttgtaatttt cagaaaattt ctccaataaa aaccagctct tagaactgat tcttcatttc
    16981 acttatttat cttcagtaac tacttcctga agataagcgt caaaaacttc ttcatctgaa
    17041 atcgtgtcag aaatgaagct tccattgcta gtgcgttctg acaagttcaa gtcttgcaat
    17101 cggctttcat agattgttcc tttattggat tggacaagca gagtttggtc gttcacatcc
    17161 acttccgtac tgaagaaatc gccaacaaat ccttgctctg caactgctcc tgccaagaag
    17221 acacgatgcg gtttgttttt caactcacgc aagacttgta atcctcgttt ggcacggctg
    17281 gttgctagaa tttcctcaat ggaaacacgt ttcaagcttc cacgctgggt caagaggtag
    17341 aaggacgaag tattacagat aaagccagat tggaggacat catcttcttt caaattcata
    17401 gccttgacac ctgctgcctt agcaccgaca accggaacct cttcgatatt gaaacgcagg
    17461 gcataaccat tttgactaac caagacaaca tcatctagtt taatcggagc cactgctaca
    17521 atctgatctg tatcgtcttt gagcttagca tacttgacag acttagatct ataggtccgc
    17581 catggagtaa attcttttcg ctctacccgt ttgatttgac caaggcgagt cgctgcaaag
    17641 taggttgtcg catcgtcaaa ctgatccagt acttccacat aaaggattgc ttcattcgtt
    17701 tcaaagtttg tgatggtttg gctcagatgc tctccgatgt ccttccaacg aatatctgcc
    17761 aactcatgga ttggtctgta gatgacattt ccaagacttg tgaacatcaa gaggtgctgg
    17821 gttgtcttgg cagattgaac aaaaatcaaa cggtcatcat cacgcttgcc aatttcttcc
    17881 aaggtggaag ccgcaaagga acgtggactg gtacgcttga tgtaacctgc cttggtcacg
    17941 ctgacgtagg tatcttcctc agcgataaga ctagctgtat caatctcaat tgctttcgca
    18001 gtgtcttcta aagaactcaa acgaggagtt gcaaatttct tcttgacctc acgaagttct
    18061 ttcttcatga gattgtacat agtcctttca tcaccgataa tagccgccag catagcaatc
    18121 ttctcacgaa gctctgcttc ttcttcctgc aagacaacca catcggtatt ggtcaaacgg
    18181 tacagttgca aagttacgat agcctcagcc tgttcttccg taaaatcata gctaactttg
    18241 aggttttcct tggcgtccgc cttattctca gaagcacgga taagagcaat gacttcatcc
    18301 aaaatcgaaa tcacacgaat caaaccttcg acgatatgga gacgtttctc agccttttct
    18361 ttgtcaaagc gtgaacgcgc caaaatcact tctcgacggt gagcgatgta gctagacagg
    18421 attggaacaa tcccaacctg acgaggtgtg aaattgtcaa tcgccaccat attaaagttg
    18481 tagttgattt gtaggtcggt gtacttaaat aagtagttga gaacaagctc agtattagcg
    18541 tctttcttaa gttcgatagc gatacgaaga ccatcacggt cagactcatc acgaacctca
    18601 gcaatcccag ctaccttgtt attaacacga acatcatcga ttttcttgac tagattggcc
    18661 ttattgattt cataaggaat ctcagtaata acgatttgtt ccttaccacc ttttagcttt
    18721 tcaatttcag tcttggaacg aacaaccacg cgccctttcc cagtctcata agctttcttg
    18781 atttcatcac gaccctgaat aatagcccct gtagggaagt ctggtccagg taagaattcc
    18841 atgagtttat caatctttgc agttgggtgg tcaatcatgt aaactgcagc atctatgacc
    18901 tcagctaaat tatggggagg aatgtctgtg gcataaccag ccgaaatccc agtcgaacca
    18961 ttgaccaaga ggtttggaaa ggctgctggc aagaccgttg gttctttctc cgtatcgtca
    19021 aagttccatg caaaaggaac tgtctttttc tcgatatcct gaagaaggta gcctgcaatt
    19081 tcagacaaac gtgcctcagt ataacgcata gccgcaggag gatctccgtc catagaaccg
    19141 ttattaccgt gcatttcaac tagaatctca cgatttttcc agttctgtga catacgaacc
    19201 atggcatcat agatagaaga atctccgtgt gggtggaaat tccccatgat gttcccgact
    19261 gacttggccg acttacggta gctcttgtca aaagtattgc tatccttatt catagaataa
    19321 agaatacggc gctgaaccgg cttcaaccca tcacgaatat ctggcaaagc ccggtcttga
    19381 ataatgtact tggagtagcg accaaagcgc tctcccatga tgtcctccag ggacatgttt
    19441 tgaatgttag acataagata caaagcccat aaaataccaa gtgaaaatag aaaattcttg
    19501 aagtaagcaa actcacaaga gaatttatct ttttcacaca gtatctaggg cgtgttcaac
    19561 tcctttcaaa gaatgtagag taggttttta tgcagtaaaa gatattttac gggaattcct
    19621 cccgtgttca gttacgataa gtaaccaaac tatcctgttt gtatttttca atatgaaaat
    19681 ctggttttcc aaaattagtc ttagtttgtg tcttagccgc tcccttaagc gcctctttga
    19741 gataagcact catagcagat tcttcattaa taatcctgca attttttcaa accaagattt
    19801 tcaaactgct ttttcacata gtcattcaca tccgactcta atttccagtt tactaacata
    19861 ttattttctt tcattaaaac actgtcgctt cttctagcgt aaacttgaca ttatcttcaa
    19921 tccatttacg gcgtggttct accttatctc ccatgagaac attgacgcgg cgttcggcgc
    19981 gcgctaaatc ttcaattgtg acacggatga gggtacgtgt ttctgggttc atggttgttt
    20041 cccagagctg gtccgcattc atctcaccaa gtcctttgta tcgttggagg gtagcgcctt
    20101 taccgaactg tttacggagt tcttctagtt ctccgtccgt ccaagcgtag gccacttctt
    20161 ctttcttgcc tttacctttg gacatcttgt aaagaggtgg gagggcaata tagacatgac
    20221 ctgcctcgac tagcggacgc atgtaacggt agaaaaatgt caagagcaag gtctggatat
    20281 gggcaccatc ggtatccgca tcggtcatga taatgatctt atcatagttg gcatcttcaa
    20341 tagagaagtc tgctccaaca cccgcaccaa tggtataaat catggtattg atctcttcat
    20401 ttttgaggat atccgccatc ttggccttgg ctgtattgac aaccttacca cgaagaggta
    20461 gaatagcctg gaacttgcgg tcacgacctt gtttggcaga accaccggca gagtccccct
    20521 caactagata gagttcattc ttagcaggat tcttagattg ggctggggtc aatttcccag
    20581 acaacaagcc cttatctttc ttgtttttct tcccatttcg gctctcatca cgcgccttac
    20641 gtgctgcttc acgagcatca cgggccttga tagccttgcg gatgaggtta gaagctaatt
    20701 ccccattttc cataaggaaa aaggtcaact tatcagccac tattccatcc acaaccgggc
    20761 gagctagggg gcttcctagt ttatccttgg tctgtccttc aaactgcaag tgttcttcag
    20821 gaactaagat agaaagaacg gccgctagtc cctcacgata gtctgaacct tcaaggtttt
    20881 tatctttttc cttgagaaga cctgttttac gtgcatagtc attcatgacc ttggtaatgg
    20941 cagacttgag tcctgtctcg tgcgttccac cgtccttggt gcgaacgtta ttgacaaagg
    21001 atagaatgtt atctgagaat ccgtcattgt actggagggc tacttccact tgaaaaccat
    21061 tgtcttcccc ttcaaagtaa agaactggcg tcaagatttc cttatcttcg ttgagataag
    21121 aaacaaaatc ttgtactcca ttctcatagt ggaactcaat cgcttcatct gttcgcttgt
    21181 ccgttaaaga caaggtcaca tttttcaaga gaaaggctga ttcattaagg cgctctgaaa
    21241 tggtattgta cttgaaatct gtcgtagaaa agatagtcgc gtcaggcata aaagtaactt
    21301 tggtgcctgt tttagacttg ggtgctgtac cgactttctt caaagtcatg acaggttttc
    21361 caccattttc aaaacgttgc ttgtaaactg cgccatcacg ggtaatttca acttctaacc
    21421 agctagaaag ggcgttaaca acggaagaac ccactccgtg aagtccacct gatgtcttat
    21481 agccaccttg accgaatttc cctccggcat gaagaatggt aaagataacc tcaacagttg
    21541 gaattcccat agcgtgcata cctgtcggca tcccacgtcc atggtcttga accgttagac
    21601 taccgtcttt attgatagtt acatcaatac gatcaccaaa cccagacaag gcttcatcga
    21661 ctgcattatc aacgatttcc caaactaggt gatgaagacc agcgccatcg gtcgatccaa
    21721 tatacatccc tggacgtttt cggaccgcat ccaacccttc tagcacctga atagcatcat
    21781 cattataatt gttaatattg atttcctttt ttgacacaag gaacctccta ttcgttcatc
    21841 tttactattc tacaggtttt ccaaggattt tgcaaaattt ttctttctcc gatgtgacaa
    21901 ttttagcaga gattctctgc ttttctttcc caattcatga tataatagga gtatgattac
    21961 aatagtttta ttaatcctag cctatctgct gggttcgatt ccatctggtc tctggattgg
    22021 acaagtattc tttcaaatca atctacgcga gcatggttct ggtaacactg gaacgaccaa
    22081 taccttccgc attttaggta agaaagctgg tatggcaacc tttgtgattg actttttcaa
    22141 aggaacccta gcaacgctgc ttccgattat ttttcatcta caaggcgttt ctcctctcat
    22201 ctttggactt ttggctgtta tcggccatac cttccctata tttgcaggat ttaaaggtgg
    22261 taaggctgtc gcaaccagtg ctggagtgat tttcggattt gcgcctatct tctgtctcta
    22321 ccttgcgatt atcttctttg gagctctcta tcttggcagt atgatttcac tgtctagtgt
    22381 cacagcatcg atcgcggctg ttatcggggt tctgctcttt ccactttttg gttttatcct
    22441 gagtaactat gactctctct tcatcgctat tatcttagca cttgctagtt tgattatcat
    22501 tcgtcataag gacaatatag ctcgtatcaa aaataaaact gaaaatttgg tcccttgggg
    22561 attgaaccta acccatcaag atcctaaaaa ataaaacgcc agttctgtac tgccccccaa
    22621 aagttagaca attaatttat ccaaaggatt tagttctgta ctgcacagga ctaagtcctt
    22681 ttagttttac cttaattcgt ttattgttgt agtaatcaat atagtctaca atagcttgtt
    22741 ccaattgatt aagtgattta aatgttttct catagccata aatttcagaa tcccaaagaa
    22801 ggactccatc ataccgttgt cttggctgtt tcccttgcgt gacatggatg cttgaattcc
    22861 cttactctct aagaaccgat gataagaatc gtgttgatat tgccagcctt gatcactatg
    22921 gagaatcgta ttctcgtagt acttctctgt gaatgcctgt tccaacattg tttgtacttg
    22981 ttctaagttg ggtgaagttg aaagattata ggcgataatt tcgctgttaa agccatctaa
    23041 aactggtgat aagtaaagct tttgagtact tgctggaatg gcaaattctg tcatatctgt
    23101 gtagcacttt tccattgttt tagagccttc aaattggcct tgaatgagat tctctgcctt
    23161 cttaccaacg tctcctttat gagaagaata ttttcgtttc tgtcgcattt tagtttgtaa
    23221 attgagtact ttcatcaagc cttgaactct tttatgattt accagataac cacgatttct
    23281 tagttctaaa tgaacccggc gataagcata atttcccttg tgttcgataa aaatggattg
    23341 aatttcagct ttaagctctt ggtccttatc tggtttgtct agctgtttca agtgatagta
    23401 gtaggtcgaa cgagctagtt taatggcttt tagaagaaga tctaacgaaa actcagtcat
    23461 taattcttga acaatttctg tctttcttct ttctcttttt cctccttcaa tcggagttct
    23521 cttaactttt ttaggatggc attctccgct ctcaggtact cattttctgc ttgaagatgt
    23581 tctaattctg tcctctcttc aggtctcttt tt
//
LOCUS       DAVKWS010000027        23388 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKWS010000027 DAVKWS010000000
VERSION     DAVKWS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.027"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            94..165
                     /locus_tag="VW852_001184"
     tRNA            94..165
                     /locus_tag="VW852_001184"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:127..129,aa:Glu,seq:ttc)
     gene            171..244
                     /locus_tag="VW852_001185"
     tRNA            171..244
                     /locus_tag="VW852_001185"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:205..207,aa:Asn,seq:gtt)
     gene            complement(285..827)
                     /locus_tag="VW852_001186"
     CDS             complement(285..827)
                     /locus_tag="VW852_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV0272337.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            1006..1773
                     /locus_tag="VW852_001187"
     CDS             1006..1773
                     /locus_tag="VW852_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV0272338.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSKKTTTLSNI
                     QSTAAYQSLTSEQQTGISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGAVTSKLVPASQLIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            1858..2196
                     /locus_tag="VW852_001188"
     CDS             1858..2196
                     /locus_tag="VW852_001188"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272339.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFFAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            2224..2508
                     /locus_tag="VW852_001189"
     CDS             2224..2508
                     /locus_tag="VW852_001189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV0272340.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLR"
     gene            complement(2639..5191)
                     /locus_tag="VW852_001190"
     CDS             complement(2639..5191)
                     /locus_tag="VW852_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV0272341.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFCTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(5253..6875)
                     /locus_tag="VW852_001191"
     CDS             complement(5253..6875)
                     /locus_tag="VW852_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0272342.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            7081..8106
                     /gene="trpS"
                     /locus_tag="VW852_001192"
     CDS             7081..8106
                     /gene="trpS"
                     /locus_tag="VW852_001192"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV0272343.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            8258..9736
                     /gene="guaB"
                     /locus_tag="VW852_001193"
     CDS             8258..9736
                     /gene="guaB"
                     /locus_tag="VW852_001193"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV0272344.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(9787..10884)
                     /gene="recF"
                     /locus_tag="VW852_001194"
     CDS             complement(9787..10884)
                     /gene="recF"
                     /locus_tag="VW852_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV0272345.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(10887..11255)
                     /gene="yaaA"
                     /locus_tag="VW852_001195"
     CDS             complement(10887..11255)
                     /gene="yaaA"
                     /locus_tag="VW852_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV0272346.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            11414..12664
                     /locus_tag="VW852_001196"
     CDS             11414..12664
                     /locus_tag="VW852_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0272347.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            12661..13944
                     /locus_tag="VW852_001197"
     CDS             12661..13944
                     /locus_tag="VW852_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0272348.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13986..14807
                     /locus_tag="VW852_001198"
     CDS             13986..14807
                     /locus_tag="VW852_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0272349.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            14818..15363
                     /gene="pgsA"
                     /locus_tag="VW852_001199"
     CDS             14818..15363
                     /gene="pgsA"
                     /locus_tag="VW852_001199"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV0272350.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            15360..16187
                     /locus_tag="VW852_001200"
     CDS             15360..16187
                     /locus_tag="VW852_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0272351.1"
                     /translation="MKSIIGVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            16172..17011
                     /locus_tag="VW852_001201"
     CDS             16172..17011
                     /locus_tag="VW852_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0272352.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            17004..17798
                     /locus_tag="VW852_001202"
     CDS             17004..17798
                     /locus_tag="VW852_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0272353.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            17857..18675
                     /gene="mreC"
                     /locus_tag="VW852_001203"
     CDS             17857..18675
                     /gene="mreC"
                     /locus_tag="VW852_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV0272354.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            18675..19169
                     /gene="mreD"
                     /locus_tag="VW852_001204"
     CDS             18675..19169
                     /gene="mreD"
                     /locus_tag="VW852_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV0272355.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            19263..20441
                     /locus_tag="VW852_001205"
     CDS             19263..20441
                     /locus_tag="VW852_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV0272356.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            20665..21444
                     /gene="rpsB"
                     /locus_tag="VW852_001206"
     CDS             20665..21444
                     /gene="rpsB"
                     /locus_tag="VW852_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV0272357.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            21523..22563
                     /locus_tag="VW852_001207"
     CDS             21523..22563
                     /locus_tag="VW852_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV0272358.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            22809..23132
                     /gene="tnpB"
                     /locus_tag="VW852_001208"
     CDS             22809..23132
                     /gene="tnpB"
                     /locus_tag="VW852_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0272359.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(23273..>23388)
                     /locus_tag="VW852_001209"
     CDS             complement(23273..>23388)
                     /locus_tag="VW852_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV0272360.1"
                     /translation="ILEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLCENR"
BASE COUNT         7444 a         3747 c         4770 g         7427 t
ORIGIN      
        1 agaaaattaa aagatatttt agagaaaaaa tctcaaaagt gattgacaat tagcaagaaa
       61 ttgatataat ggttatatct gctacagata gaaggtccgt tggtcaaggg gttaagacac
      121 cgccttttca cggcggtaac acgggttcga atcccgtacg gactatttta tccgccatag
      181 ctcagttggt agtagcgcat gactgttaat catgatgtcg taggttcgag tcctactggc
      241 ggagtataga taaaagggaa cacagctgtg ttcctctttt tgtatcaatt tgtatcacca
      301 agcattttca taaggaagtc tgttatttct tgagaacttt ctttttttcc atgtgcaatc
      361 caagtttggc agacaccaaa aagtgcatga gttagataga tgctactata ttctaattca
      421 gtggtattta gattcagttg cataaatcgc ttttgtaaat ctgtactaag catgatatga
      481 agtttatttc gtaagaaatt ttggatttct ttagtcccat tttcagaaag aagggcagcc
      541 agaagtggtt ctgactctag atattcaaaa acttctaaaa tagcgtctct tttgtgatga
      601 gcatgttttt gaaaaatata ttcaaatgta tggaatagct tgctttgata gtgctcaatc
      661 atatcatact tatccttata gtgagtatag aagctggaac gactaattcc ggctttttct
      721 actaatttga cagtagaaat tttatcaaat ggctgttcca tcagtaattg taccatagca
      781 ttttcaatag ttcgctttgt ttttaagcgt ttgttacttt cttgcatatt tcctccttgt
      841 aaacaaatta gactatatgt ctaaaaatag attttttatc ttgtaattta gattttttaa
      901 tgtataatct attatatcaa aattttagac aatatgttta aaaaaggaga aactaagttt
      961 aaagaatgga aagcaattta aaaaaaacca acctttatta ttgtcatgat cgggatttct
     1021 cttattccag atctgtacaa tatcatattt ttgtcatcaa tgtgggatcc atatgggcaa
     1081 ttgtctgact tacctgtggc agttgtaaat aatgataaag aggcttccta taatggtaat
     1141 actatggcaa taggaaaaga catggtgtcc aatttaaaag aaaataaaac cttggatttt
     1201 cattttgtag atgaagagga aggaaagaag ggattggaag atggcgatta ctatatggta
     1261 gtgactttac caagtgattt atctaaaaaa acaactacat tatccaatat tcaatcgaca
     1321 gcagcttatc aatcattgac aagtgagcaa caaactggga taagtgattc tgtatctcaa
     1381 aattcaactg atagtattca atcggctcag tcaattgtag ctttagtaca aggtttacag
     1441 ggaagtttag aaaacttaca aaatcaatct tctaatcttt cgactttaaa aaatcaagct
     1501 aatcaagtat tacctattac ttctacttct ttgataggat tgtcaagtgg attaacagag
     1561 atacaaggag ctgttactag caaattagtt cctgccagtc agttgattgc atcaggtgta
     1621 aacgcatata ctacaggtgt tgataaagtt tctcagggcg caagtcaact aagtgaaaaa
     1681 aatgccacct tgacaggtag tttggataaa ctagtttcag gctcaaacac cttgacacaa
     1741 aaatcttcta gattgacagc aggagttggt taattacaat caggatctgg gcaattagca
     1801 gacaaatcca gtcagttact ttcaggtgct tctccattag agaatagagc taataaattg
     1861 gcagatggat ctgggaaact agcagaaggt ggaacaaagt taacttctgg attggaagat
     1921 ttacagacag gacttgcttc tttaggacaa ggactaggta atgctagtga tcaactcaaa
     1981 tcagtatcaa cagaatctaa aaatgcagag attttgtcaa atccactcaa tctttcaaaa
     2041 acagacaatg atcaagttcc tgtaaatgga atcgcaatag ctccttatat gatatcagtt
     2101 gctctttttt ttgcagcaat atcaacaaat atgatatttg cgaaattgcc ttcaggacgt
     2161 catccagaga gccgttgggc ttggttgaaa tcttgagctg aaataaatgg tattatagct
     2221 gttttggcag gaattttggt atatggagga gttcagctta ttggtttaac tgctaatcat
     2281 gagatgagaa tatttattct catcatccta acaagtttag tattcatgtc tatggtgacc
     2341 actttagcaa cgtggaatag ccgtatagga gcttttttct cacttatttt gcttttacta
     2401 cagttagcat caagtgcagg tacttatcca cttgctttga caaatgattt ctttagatct
     2461 attaatccct ggttaccaat gagctattca gtttcgggat tacgataaac aatctctatc
     2521 aacaagtcat tttcctagct gtcatactag ctctatttac tggtttaggt atgctagcct
     2581 atcaacataa gaaaatggaa gaagattaaa aaaatcgacc gattaactgg tcgatttttt
     2641 atgccttaga tgactttcgt ctgtgattat agattccaaa tagtaagaga gaagtaaagg
     2701 aacagattgc tccagtaata aaaccattgg gaatgaagga aagtgtaata gttcctttcc
     2761 ccttgggaat gtcaactttc ataaatccag tttgagcttg tttaatttct attttcttac
     2821 catcttggta ggcagaccaa cctttgtcat aaggaatggt gaagaaaata gatgtatctt
     2881 gttggacatc atatgtagca aaaaccttgt ttttagaagt tgatactgtg acaggttgtt
     2941 ctttaatttt ttgaattgcc tcggtgaaag ttttggtatc taaacgatag aaggtaggag
     3001 attcaaatga tacttgtgaa tttccaggga aactaacatt gatattgaaa gtttttttct
     3061 ctttagtata tcctagatta aagaaggaga agacattatc agttgtaaaa gtcttttttt
     3121 caccatttac aagtatgtca accttctttt gtttatcgtt agaaaagtga aggtttatga
     3181 aagagagata aacttggctg ttttctggaa cttcaatttg atactggatt gctgcatctt
     3241 catttgaaga acttgtgaca ctaatcaaat cattagtatt ttctattttt tctgtttttt
     3301 cataaggtat tggagaaaaa taatcaaaat tgacgttagc aagttgattt aaaaatgagg
     3361 cctgattatc caaggtatgt tcattgaact tgacatcatt gtaaacagat tgactagcaa
     3421 ctgcaatcgg aagagagtat tgattttcat atagggtaag attatctttt tgatagatat
     3481 ctttaaagcc atacttatca ataggactgt ctgagatatt gtactggata ccaaataaac
     3541 tatcagccaa aatactatta tttgcatatc ggagattgag attagtccca gaggatttaa
     3601 aaccaagttt atctaaagta gagcttgatg aacgatttcg aacagatgaa aattgagaga
     3661 ttccattgta gttgaatttc atactgtcat ttcctgtctg agtttgtagt ttttcagtac
     3721 gagtaaattg atttccaata tatgttgaga aagattccat agctgggata tctcgactat
     3781 aagcacttcg agaagcaaat ccccattcct tagcaattcc gtccatttga gatgaagcat
     3841 ttaaactcat ttcaaccagt ataaataaag agattagaat ggcaaataga ttcacagata
     3901 taaacttttt gataactgca aggagtaaaa gagaatagac aaccaaaaat tcaagagtaa
     3961 gcagaatatt caaatctgtt aaaaaagaat aatgcgattt tagatagatg gtagctaaaa
     4021 atcctgctac tacaagaaaa agcgaaacta aaaaattcca gactttaagt tctttcagac
     4081 gctttaagac ttctgctgct gtgtaaatta acaaggtaga gaaaatccaa gcatagcgat
     4141 gtaaaaacat gtttggagta tgcatgcctt gccaaaataa gtcaagagct tctatgtaaa
     4201 agcttgcaat tagaaatgca aagaatatta catatatgag tttcacgtga aacttaatag
     4261 atttcagcgt acaaaataaa atggtcaaaa taaagggaaa tagtccaaca aaaatcattg
     4321 ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa ttgcttagca aagagatcaa
     4381 gataccagct actttcagtt tgaaactttg taacttcagt caatttttcc ccatgtgtct
     4441 gtaaatcaaa tagagtggga agagtcataa tcaaactagc cataccagct aaaaaggaga
     4501 taactatgaa atcaagaaca gatgattttc gagtcttaaa gtcccacgaa atttgacaga
     4561 gataccagaa aataagaaac aatactgtca tatatccaaa ataataattt tgaataaata
     4621 agattgacag acttgtaaag tacaatagga gtttcttttc agttatcagt agatgtaaac
     4681 cagttataat taaaggaatc aagataaaaa catctagcca ggtttttatc tctaattgac
     4741 tgacagtgaa actcatcaga gcataggaag tagataaggc tagttttaaa gtctgaggga
     4801 tagattgaaa caatttattc aaactaaaaa aggttgacag accaatcaat ccaaatttta
     4861 agagagtggt cagatagaca gcatctggca tattcgttag atcaaaaaag taaactagag
     4921 gcgcgagaaa actacccaag taataactag atagggcata gaagtttagc cctagaccac
     4981 ttgtaaaggt gtaaaacaga ctactatttc catgtaggat atttcgtaag gctacatcaa
     5041 aaataacgta ttgatgaaag ccatctccta atagaggaga gttgtcgcta ttccagtaga
     5101 taccttgaga tagatatact ccagacataa tcactacagg aatgatgaaa gaaataaaat
     5161 aggttcgata tgtttttaaa aatgatttca tgttacctcg tagaatgata gaaaactcag
     5221 ttggttaacc caactgagtt ttgaagtttt atttagtctt tccaaagttc tttaactttt
     5281 gcttgtactt ctgcattttc taggaattca tcgtaggttt catcgatacg gtcaatgacg
     5341 ccatttttag ataagacaat gatatggtta gccaaagttt gaataaattc gtggtcatgg
     5401 ctagcaaaga tgattgattc tttaaagttt ttcaatccat cattcaagct tgagatagat
     5461 tccaagtcca agtgatttgt tggatcatca agtacaagga catttgattt taagagcatg
     5521 agttttgaaa gcatgacacg aactttttct ccccctgaca agacatttac aggtttgtta
     5581 acttcatctc cagagaagag catacggccg aggaaaccac gtaggaaagt attgtcatct
     5641 tcttctttac ttgcgaattg acgcaaccag tcaagaattg attctcctcc tgcaaaatca
     5701 gctgagttat cttttggtag gtaagattga ctagttgtaa ctccccactt gacagttcct
     5761 tcatagtcaa tatctcccat gattgcacga attaatgcag tcgtttgaat atcattttgt
     5821 ccaataagtg ctgtcttatc atctggacgc aagatgaaac taatattatc caagatagtt
     5881 tcaccatcaa tctttacagt tagattttct actgtcaaga gatcattacc aatctcacgt
     5941 tccgctttaa agttgataaa tggatattta cgactagatg gtacaatctc ttctagctca
     6001 atcttatcaa gcattttctt acgtgatgtt gcctgccttg acttagaagc attggcagag
     6061 aaacgagcaa cgaattcttg caattgttta attttttctt ctgctttagc attacggtct
     6121 gctagcaatt tagcagcaag ctcagaagat tccttccaga agtcgtagtt tccgacatag
     6181 agtttgattt ttccaaagtc aaggtcggcc atgtgagtac aaactttgtt taagaagtga
     6241 cggtcgtggg atactacgat aactgtgtta tcaaagtcaa tcaagaagtc ttctaaccaa
     6301 gtaatcgatt ggatatccaa accgttagta ggctcgtcca agagaagaac atctggttta
     6361 ccaaaaagtg ctttggcgag gagaaccttt actttttcac cgttggccaa ttcgctcatg
     6421 ttttggtagt gtaattcttc tggaatgttt aggttttgaa gtagttgaga ggcttcactc
     6481 tctgcttccc aacctccaag ttcggcaaac tctccttcga gttcggcagc acgaaccccg
     6541 tcctcgtctg agaaatcttc cttcatgtag atagcatctt tctctttcat gatgctataa
     6601 agtttttcat ttcccatgat aacgacatca atggcacgtt catcttcgta gtcaaagtga
     6661 ttttgacgaa gaacagagag acgttcatct ggaccaagag agatgtgacc agtagtaggt
     6721 tcgatatctc cagctaaaat ttttaaaaag gttgattttc cggcaccatt agcaccgatt
     6781 aatccgtaag tatttccttc tgtaaatttg atattgacat catcaaaaag tttgcgatca
     6841 ctaaaacgta gtgaaacatc agatactgta agcaatattt ttctcctata tgtgtaatat
     6901 atttattcta ctagaaaata cagaaatatt caaattttta tttgtcaatt ttgtgtaaat
     6961 tatatttaca gtatccttta cacaaatctg taaaaagcaa ggctgattta ttttgataaa
     7021 ttacggttat ttcattaaaa aaatgctata attgaaagga ctatatcgaa ggagaacaaa
     7081 atgactaaac ccattatttt aacaggagac cgtccaacag gaaaattgca tattggacat
     7141 tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag aggataagta tgatatgttt
     7201 gtgttcttgg ctgaccaaca agccttgaca gatcatgcca aagatcctca aaccattgta
     7261 gagtctatcg gaaatgtggc tttggattat cttgcagttg gattggatcc aaataagtca
     7321 actattttta ttcaaagcca gattccagag ttggctgagt tgtctatgta ttatatgaat
     7381 ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca agacagagat ttctcagaaa
     7441 ggatttggag aaagcattcc gacaggattc ttggtctatc caatcgctca agcagctgat
     7501 atcacagctt tcaaggctaa ttatgttcct gttgggacag atcagaaacc aatgattgag
     7561 caaactcgtg aaattgttcg ttcttttaac aatgcatata actgtgatgt cttggtagag
     7621 ccggaaggta tttatccaga aaatgagaga gcagggcgtt tgcctggttt agatggaaat
     7681 gctaaaatgt ctaaatcact aaataatggt atttatttag ctgatgatgc ggatactttg
     7741 cgtaaaaaag taatgagtat gtatacagat ccagatcata tccgcgttga ggatccaggt
     7801 aagattgagg gaaatatggt tttccattat ctagatgttt ttggtcgtcc agaagatgct
     7861 caagaaattg ctgatatgaa agaacgttat caacgaggtg gtcttggtga tgtgaagacc
     7921 aagcgttatc tacttgaaat attagaacgt gaactgggtc ctattcgtga gcgccgtatt
     7981 gaatttgcta aggatatggg agaagtttat aatatgattc aaaaaggtag tgaaagagcg
     8041 cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg caatgggact tcattacttt
     8101 aactaagttt attttgcaag aaactatctt ttctatctcg aagaaaagat agttttttat
     8161 ttacccctgt aacatttgac aaatttttat agaatggtat gatagataca atataaaaag
     8221 agtcaagctc aaaaagaaag aaaagaggaa acttcgaatg tctaattggg acactaaatt
     8281 tttgaaaaaa ggttttacct ttgatgatgt attgcttatt ccagctgaaa gtcatgtgtt
     8341 gcctaacgat gcagatttaa caactaaatt ggcagataat ttgactttaa atatcccaat
     8401 tattaccgct gccatggaca cagttacaga gagtcaaatg gccattgcta ttgctcgtgc
     8461 aggcggtctc ggagttatcc ataaaaacat gtcaattgct caacaagcag acgaggttcg
     8521 taaggtaaaa cgttctgaaa atggagttat tattgatccg ttcttcttga cgcctgaaca
     8581 tacaattgct gaagcagatg agcttatggg tcgttaccgc atcagtggtg ttccagttgt
     8641 tgaaacactt gaaaatcgta aattggttgg tattttgaca aaccgagatc ttcgttttat
     8701 ttcagattat aatcaaccaa tttcaaacca tatgactagt gaaaatcttg ttactgctcc
     8761 tgtgggtacg gatcttgcaa cggctgagag tattcttcaa gagcatcgta ttgaaaaact
     8821 tccgttggtc gatgaagaag gcagtctttc tggtttgatc actatcaaag atattgaaaa
     8881 agttattgag tttccaaatg cggctaaaga tgagtttggt cgtcttctag ttgcaggtgc
     8941 agtaggtgtt acttcagata catttgaacg tgcagaggct ctttttgagg caggagcgga
     9001 tgcgattgtt attgatactg cacatggtca ttctgcaggt gtcttgcgta aaattgccga
     9061 gattcgtgct catttcccag atcggacttt gattgctgga aatattgcta ctgctgaagg
     9121 tgcacgtgcc ctttatgaag cgggtgtaga cgttgttaag gttggtattg gaccaggttc
     9181 tatctgtact actcgtgtga ttgctggtgt tggtgttccg caagtaacag ctatctacga
     9241 tgctgcagct gttgcgcgcg aatatggtaa aacgattatt gctgacggtg ggatcaagta
     9301 ttctggagat attgtaaaag cacttgctgc aggtggaaat gctgttatgc ttggatctat
     9361 gtttgctgga actgatgaag ctccaggcga aactgaaatc ttccaaggac gtaaattcaa
     9421 gacttaccgt ggtatgggat caattgctgc tatgaagaaa ggttcaagcg accgttattt
     9481 ccaaggttct gtcaatgaag caaacaagct tgttccagaa ggaattgaag gtcgtgttgc
     9541 ttataaagga gcggcagctg atattgtttt ccaaatgatt ggtggtattc gctctggtat
     9601 gggttactgt ggtgcagcta accttaaaga actacacgat aatgctcaat ttattgaaat
     9661 gtctggtgct ggtttgaaag aaagccatcc tcatgatgtg caaattacta atgaggcacc
     9721 aaattattct atgtaaaaaa caatgaaaag aactccagtg aaaacaggag ttcttttaca
     9781 atgttgtcaa tttccattta cagcagcttt accatcctga atagtgaaga tacttagatt
     9841 ttctggcaga ttttgaagat ggtctaagct tgttgttgtg ataaaggttt ggattgattg
     9901 agaaatcgtt tctaataatt ttaactgtct agtgttgtca agttcactca tcacatcgtc
     9961 aagcagtaat ataggagatt ctgtggtaat gctttccatt aattcgattt ctgctaattt
    10021 tatcgagagg acgagactac gatgttgacc ttggcttccg aaactagcat ccatcccatt
    10081 tatataaaaa gaaatgtcat ctcgatgagg accgacacca gtattctttt taaataaatc
    10141 tctggatcta cttttttcta aagcaatttt gaaagattcg gataagtttt gtttgtcagt
    10201 tatattgaca gaagattgat aggatattga caactcttcg atctgattag agagttcaaa
    10261 atgtttctta cgcccaaatg attctagttt ttttatgaaa tctaagcggt gattcattac
    10321 acgacatcca taatcaacta gctgatcatc taacacagaa aggaatgttt catctatttt
    10381 ttgatctgat tttaggtaag tgtttctttg ctttaggatg tggttataat tggttaagtc
    10441 agataagtag attggcttaa tttgcccaag ttccatatca atgaattttc gtcgaatcga
    10501 aggtgctcct ttaattagtt gtaaatcttc aggagcaaat aagacaacat tcatgtgtcc
    10561 tacataatct gaaaggcgtg cctgttttaa gtgattaact tttgttacac ggcctttttg
    10621 tgttagttcg atttctaggg gaatggatcc agtttttttc tgaacgagac ctgaaagatg
    10681 aagttgttcc tcatcaaaat gaatgagatt tttatctgtt cgagttcgat gactacgcgt
    10741 taaggctaaa aaatagatag cctctaacat gtttgttttt ccttgtgcat tacgtcccaa
    10801 aaagacattc aatttaggat taaagtctat tttcgtttct ttgtaattac gaaaagtctt
    10861 gagagataga tgttgtagcc acatgattat ctaccaggga atcgtggagc ttgttggctt
    10921 ttaggtgatg aagcaggttt cgatttgtct tttttaactc ccttattcat ctttttgaca
    10981 agtttagcga tccgttcttt ttcaacttta tcagcttggt attcatcttg ttcttcagaa
    11041 gtaggttgtg tcaacaagat gtcaatgttc atgtcaggga tgtcaacttt atcgccaata
    11101 cgaagttttt ttccacgacg actctctaat tccctattaa agtaaacaga atgttcagag
    11161 agaaatgatt taatagctcc tccgctatgt gtaattccaa gttctttgag tagtgcttgg
    11221 agggtaataa attcttcaaa taatttgtat tccataattc acctcaatct ttggtattat
    11281 accatatttt cctaaaaata gtgagagaga cagcgagaaa tgtaagcgat ttttatttca
    11341 aaagtgttac agggaacaag aaaatttcag gttttcgtga tataatagaa gtctgtatat
    11401 aaggaggtaa atcatggagt tagtgcatgg aatttcaaca cattttatcc aatcaaaaaa
    11461 gtttaaaaca aacaaaatta ccgtgcgttt taccgctcca ttatcccttg atacgattgc
    11521 aggtcacatg ttgagtgcaa gtatgctaga gactgctaat cagatgtacc ccacttctca
    11581 agatttgagg agacacttgg ccagtctata cggtacagat atgtcaacca attgtttcag
    11641 aagagggcaa agccacatta tagaattgac atttacctat gttcgtgatg agtttttaag
    11701 taggaaaaac gtgctaacct ctcagatttt ggaacttgta aaagaaactc ttttttcacc
    11761 cgtagtagtt gataatgggt ttgatccggc cttatttgaa attgagaaaa aacaattgct
    11821 agcaagttta gcagctgata tggatgattc tttttatttt gcacataaag aattggataa
    11881 attgtttttt catgatgaac gtcttcaatt ggaatatagt gatttacgaa atcgtatttt
    11941 agctgaaact ccacaaagtt cttattcttg tttccaagaa tttttagcca atgatcgaat
    12001 agatttcttt ttcctaggtg attttaatga ggttgaaatt caaaatgtat tagaatcatt
    12061 tggctttaaa ggtcgaaaag gagatgtgaa ggttcagtat tgtcaacctt attctaatat
    12121 ccttcaggaa ggtatggttc ggaaaaatgt gggacaatct attttggaat taggttatca
    12181 ttaccgttct aaatatggtg atgagcaaca tttacccatg attgtaatga atggtttact
    12241 tggtggattt gctcactcta agctctttac aaatgtccgt gaaaatgctg gattagctta
    12301 taccatttca agtgagcttg atttatttag tggattcttg aggatgtatg ctggtatcaa
    12361 tcgagaaaat cgtaaccagg ctcgtaaaat gatgaataat caactgcttg atttaaaaaa
    12421 aggttatttt acagagtttg agttaaatca gaccaaggaa atgattcgtt ggtcgttgtt
    12481 actttctcaa gataatcaat cttcattgat tgaacgtgct tatcaaaatg ccttatttgg
    12541 aaaatcttca gcagacttta aaagttggat tgcaaagctt gaacagattg acaaagatgc
    12601 tatttgtaga gtagctaata atgtgaaact acaagcgatt tactttatgg aaggaataga
    12661 atgacaaagg ttgtttttga agaaaaatac tatccagctg taaaagaaaa ggtttatcga
    12721 actcgtttgg ccaacggatt gacagttgct cttttgccta aaaaggaatt taaagaggtt
    12781 tacgggagtg taactgtaca gtttggttcg atagatacgc ttgtcacaga agttgacgga
    12841 gatgtaaaac aatatcctgg aggaattgct cattttcttg aacataaatt atttgagaga
    12901 gaagattcta gtgatttgat gtcggctttt acgagtctag gtgcagatag taatgccttt
    12961 acaagcttta caaaaacaaa ctatcttttt tcagcaacgg attatttttt agaaaattta
    13021 tatttacttg atgaattggt aacatcagca cactttactg aagcttccat tctgacagag
    13081 caggatatta ttcagcaaga acgagaaatg taccaagatg atccagattc gtgtttattc
    13141 ttttcaactt tagcgaattt gtatcctggt acacctttag caactgatat agttggaagt
    13201 gaggagtcca tttcccaaat caatctaact aatttgcaag aaaattttac aaagttttac
    13261 aaacctgtaa acatgtctct gtttttagtt ggtaattttg atgtggagcg agtacaggac
    13321 tattttgaaa gcaaagaact gaaagattca gattttcagg aagtagcaag agaaaagttg
    13381 tttttacagc ctgtaaagcc aacagatagt atgagaatgg aagtatcttc tcccaaacta
    13441 gcgattggag ttagaggtaa gcgagaagtt tctgaagcgg attgctatcg acatcatatt
    13501 ttattaaaat tattgtttgc aatgatgttt ggttggactt cggatcgttt tcaaaaatgt
    13561 tatgaatcag gtaaaattga tgcgtcctta tctctggaag ttgaaataac aagtcgcttt
    13621 cattttgtca tgttgacaat ggatacgaaa gagccagttg ctttgtctca tcaatttagg
    13681 aaggctattc gtaattttac aaaggattta gatattacag aggaacattt agatattatc
    13741 aaaagagaga tgtttggcga atttttcagt agcatgaact ctcttgaatt tattgcaacg
    13801 caatatgatg cttttgaaaa tggtgagaca atttttgatt tgccgaaaat tttacaggaa
    13861 attactttag aggatgtcct tgatgctgga catcatttaa tagatgatgg tgacatagtt
    13921 gattttacaa tattcccatc gtagtaacct atcataatag acactagaaa gaagggatga
    13981 caagtatgag aaaaaaaaca attggagagg ttttacgatt agctagaatc aatcagggat
    14041 tgagtttaga tgaattgcag aaaaagacag aaatccagtt agatatgttg gaagcaatgg
    14101 aagcagacga tttcgatcaa cttccaagtc ctttttacac gcgttctttc ttgaaaaaat
    14161 atgcatgggc tgttgagtta gatgaccaaa ttgttttgga tgcttatgat tctgggagta
    14221 tgattactta tgaggaagta gatgttgatg aagatgagtt gacaggtcgt agacgttcaa
    14281 gtaagaaaaa gaagaaaaaa acatcatttt tacctttatt ttattttatc ctttttgctt
    14341 tatcgatttt aatttttgtg acttattatg tttggaacta tattcaaact caaccagagg
    14401 agccttctct ttctaattac agtgtggttc aatcaacaag ttcaactagc tctgttcccc
    14461 actcctcaag tagtagttct tctagtatag aatcagctat aagtgtatca ggcgaaggaa
    14521 atcatgtaga aatcgcttat aagacaagta aggaaacagt taaattgcaa ttggcagttt
    14581 cagatgttac aagttgggtc agtgtttcag aaagcgaact tgagggcggt gtaaccttat
    14641 cgccaaagaa gaaaagtgca gaagcaacag ttgcaactaa aagtcctgta acaattacgt
    14701 taggtgttgt aaaaggtgtt gatttgacag tagataatca gactgttgat ttatcgaaat
    14761 taacagctca gactggacaa atcactgtaa cctttactaa aaattaagga aaaacgaatg
    14821 aaaaaagaac aaattcccaa tctcttaaca ataggtcgaa ttctctttat acctattttt
    14881 atctttattt taacgatagg aaattcgata gagagtcata tagttgcagc tattatcttt
    14941 gctgttgcca gtattaccga ctatttagat ggatatttag ctcgtaaatg gaatgtggtc
    15001 agtaattttg gtaaatttgc agatcctatg gcggataagt tactagttat gtcggctttt
    15061 attatgttga ttgagttagg tatggctccg gcttggattg ttgcagtgat tatctgtcgt
    15121 gagttagctg tgacaggttt aaggctttta ttggttgaaa ctggtggaac aattttagca
    15181 gcagcaatgc ctggaaaaat taaaactttt agtcagatgt ttgctattat tttcttgcta
    15241 ttacattgga ctttacttgg tcaagttcta ctttatgtag ccttattttt cactatctac
    15301 tctggctatg actatttcaa gggtagtgcc tatgtattta aagggacatt tggttcgaaa
    15361 tgaaatcaat aattggtgta aaaaatcttt cttttcgcta taaagaaaat cagaactact
    15421 acgatgtgaa ggatattacg tttcacgtga aacgtggaga atggctttcg attgtagggc
    15481 ataatggtag tggtaaatca acgacggttc gattaattga tggcttactg gaagcagaat
    15541 ccggagagat tgtaattgat ggccaacgtt tgactgagga aaatgtttgg aatatacgtc
    15601 gtcaaatcgg tatggttttt caaaatccag acaatcaatt tgttggagcg actgttgaag
    15661 atgatgttgc ttttggtttg gaaaatcagg gactttctcg tcaagaaatg aaaaagagag
    15721 tggaagaagc tctggcttta gttggcatgt tggactttaa aaagagagag ccagcgcgtc
    15781 tatcaggtgg ccaaaagcaa cgtgtggcca ttgcaggtgt tgtagcccta agaccagcta
    15841 ttttaatctt agatgaagca acgagtatgt tggatcctga ggggcgtaga gaacttattg
    15901 ggacagtaaa aggaattcga aaagactatg atatgacggt catttctatt acccatgatt
    15961 tggaagaagt cgccatgagt gatcgcgtat tggtcatgaa aaaaggggaa attgaatcaa
    16021 ctagtagtcc aagggagctt ttctctcgaa atgatttaga tcaaattgga ttagacgatc
    16081 cttttgctaa tcaattaaaa aaatctttga gccagaatgg ctatgattta cctgaaaatt
    16141 atttgacaga aagtgagcta gaggataagc tatgggaatt gctctagaaa atgtgaattt
    16201 tatatatcaa gaaggtactc ccttagcttc agcagctttg tcggatgttt ctttgacgat
    16261 tgaagatggc tcttatacag ctttaattgg gcacacaggt agtggtaaat caactatttt
    16321 acaactctta aatggtttat tggtgccaag tcaagggagt gtgagggttt ttgatacctt
    16381 aatcacctcg acttctaaaa ataaagatat tcgtcaaatt agaaaacagg ttggcttggt
    16441 atttcagttt gctgaaaatc agatttttga agaaacggtt ttgaaggacg ttgcttttgg
    16501 accgcaaaat tttggagttt ctgaagaaga tgctgtgaag actgcgcgtg agaaactggc
    16561 tctggttgga attgatgaat cactttttga tcgtagtccg tttgagctgt cagggggaca
    16621 aatgagacgt gttgccattg caggcatact tgccatggag ccagctatat tagtcttaga
    16681 tgagccaaca gctggtctag atcctctagg gagaaaagag ttgatgaccc tgttcaaaaa
    16741 actccaccag tcagggatga ccatcgtctt ggtaacgcat ttgatggatg atgttgctga
    16801 atatgcgaat caagtctatg taatggaaaa gggacgttta gtaaaggggg gcaaaccaag
    16861 tgatgtcttt caagacgttg tttttatgga agaagttcag ttgggagtac ctaaaattac
    16921 ggccttttgt aaacgattgg ctgatagagg cgtgtcattt aaacgattac cgattaagat
    16981 agaggagttc aaggagtcgc taaatggata gtatgatttt agggcgttat atcccagggg
    17041 attcgattgt tcaccgattg gatccacgta gcaaattgct tgctatgatg ctactgattt
    17101 tgattgtgtt ttgggctaat aaccccttga cgaatctgat tctttttata gcgacaggga
    17161 tatttattgc cttgtcagga gtatctcttt cattttttat tcagggcttg aagtctatgt
    17221 ttttcttgat tgccttcaca actatttttc aactattttt catttctaat gggaatgttt
    17281 tatttgagtt ttcgtttgtg agaatcacgg attatgcttt gcaacaagct gggattattt
    17341 tttgtcgctt tgtattgatt attttctttt caactttgtt aaccttaaca accatgcctt
    17401 taagtttggc atcagctgtc gaagctttat tagcaccttt aaagcgtgtg aaagttccag
    17461 ttcatgaaat tggattgatg ctgtctatga gtttgcgttt tgtcccaacc ttgatggatg
    17521 atacgacgcg gattatgaat gcacagaaag ctcgtggagt ggattttgga gaaggaagca
    17581 tcgttcaaaa agtaaaggcg atgattccca ttttgattcc tctttttgcg acaagtttaa
    17641 aacgtgcaga ttccttggct atcgctatgg aagcgcgtgg ctatcagggt ggaaaaggca
    17701 gaagtcaata ccgacaattg aaatggactc taaaggatac gttgaccatt cttgttattc
    17761 tcgtacttgg ttgttgttta tttttcttaa aatcttagta gctttcagga attgataaaa
    17821 agttactgta acagtttttg atataaaggt aggaatatga accgttttaa aaaatcaaaa
    17881 tatgtcatta ttgtttttgt cactgttctg cttgtgtcag ctctcttagc gacgacttat
    17941 tcaagtacaa ttgtgacaaa attaggagat ggaatctcat tggttgatag agttgtacaa
    18001 aaaccttttc agtggtttga ttctgtcaaa tcagatttgg ctcatttgac acgaacatat
    18061 aatgaaaatg aaagtttgaa gaaacagctt taccaattag aagttaaatc aaatgaggtg
    18121 gaaagtttaa agacagaaaa tgaacaactg cgccaattgc ttgatatgaa gtctaaattg
    18181 caagccacaa agactttagc agcagatgtt attatgcgtt ctccggtatc ttggaagcag
    18241 gagttgacct tagatgcagg tagatcaaaa ggtgcttctg agaacatgtt agctattgca
    18301 aatggtggct tgattgggag tgtttcaaaa gtagaggaga actctactat agtcaacctt
    18361 ctgacaaata cggaaaatgc tgataagatt tctgttaaaa ttcaacatgg ctctactaca
    18421 atttatggaa ttattattgg ctatgacaag gaaaatgacg ttcttaaaat tagccaatta
    18481 aatagtaata gcgatattag tgcgggagat aaggtgacta ctggtggatt aggaaacttt
    18541 aacgttgctg atattcctgt tggtgaagtg gttgccacaa cgcatagtac agactatttg
    18601 acacgagaag taactgttaa attgagtgca gatactcata atgtagatgt gatagaatta
    18661 gtggggaatt cataatgaga cagttgaagc gagttggagt atttttattg cttcctttct
    18721 ttgttctaat tgacgcccat attagccagc ttctgggctc atttttcccc catgtacatt
    18781 tggctagtca ttttcttttt ctatttctct tatttgagac gatagaagta tcagagtatc
    18841 tctacctagt ctattgtttt gttataggct tggtttatga tgtttacttt ttccatctaa
    18901 tagggattac aactctctta tttatcttat tgggagcctt ccttcataaa ttgaatagtg
    18961 ttattttgtt gaatcgttgg acaagaatgc tagctatgat tgtgctgaca ttcctgtttg
    19021 aaatgggtag ttatcttttg gcttttatgg tagggttgac agtagatagc atgtcgattt
    19081 ttatagtcta tagcttggta ccgacgatga ttttaaattt tttatggatt actgtttttc
    19141 aatttatttt tgaaaaatat tatctataag aacgacatat aaatgtaaca aaggcgtaat
    19201 atttattagg cctttttttg gtatactagt attgtcttta aaagaaggag tatctacgta
    19261 atatgaagaa aaaaatctta gcgtcacttt tattaagtac agtaatggtt tctcaagtag
    19321 ctgttttaac aactgcgcat gcagaaacga ctgatgacaa aattgctgct caagataata
    19381 aaattagtaa cttaacagca caacaacaag aagcccaaaa acaagttgac caaattcagg
    19441 agcaagtatc agctattcaa gctgagcagt ctaacttgca agctgaaaat gatagattac
    19501 aagcagaatc taagaaactc gagggtgaga ttacagaact ttctaaaaac attgtttctc
    19561 gtaaccaatc gttggaaaaa caagctcgta gtgctcaaac aaatggagcc gtaactagct
    19621 atatcaatac cattgtaaac tcaaaatcaa ttacagaagc tatttcacgt gttgctgcaa
    19681 tgagtgaaat cgtatctgca aacaacaaaa tgttagaaca acaaaaggca gataaaaaag
    19741 ctatttctga aaaacaagta gcaaataatg atgctatcaa tactgtaatt gctaatcaac
    19801 aaaaattggc tgatgatgct caagcattga ctacgaaaca ggcagaacta aaagctgctg
    19861 aattaagtct tgctgctgag aaagcgacag ctgaagggga aaaagcaagt ctattagagc
    19921 aaaaagcagc agctgaggca gaggctcgtg cagctgcggt agcagaagca gcttataaag
    19981 aaaaacgagc tagccaacaa caatcagtac ttgcttcagc aaacactaac ttaacagctc
    20041 aagtgcaagc agtatctgaa tctgcagcag cacctgtccg tgcaaaagtt cgtccaacat
    20101 acagtacaaa cgcttcaagt tatccaattg gagaatgtac atggggagta aaaacattgg
    20161 caccttgggc tggagactac tggggtaatg gagcacagtg ggctacaagt gcagcagcag
    20221 caggtttccg tacaggttca acacctcaag ttggagcaat tgcatgttgg aatgatggtg
    20281 gatatggtca cgtagcggtt gttacagctg ttgaatcaac aacacgtatc caagtatcag
    20341 aatcaaatta tgcaggtaat cgtacaattg gaaatcaccg tggatggttc aatccaacaa
    20401 caacttctga aggttttgtt acatatattt atgcagatta atttacagag ggactcgaat
    20461 agagccctct tttcaggttt taccgtgaca atccctatta aaaattatat caaaatagct
    20521 tgaaaatatt ggaaaagtat ggtaaaatga aaattgtcgt gtgaacgata atactcattc
    20581 ttgatgaatt gtgaagcagt tgcccttggg tcgttttgcg agttgaagtc aagaagagga
    20641 aaaaaacaaa aaggagaaat actcatggca gtaatttcaa tgaaacaact tcttgaggct
    20701 ggtgtacact ttggtcacca aactcgtcgc tggaatccta agatggctaa gtacatcttt
    20761 actgaacgta acggaatcca cgttatcgac ttgcaacaaa ctgtaaaata cgctgaccaa
    20821 gcatacgact tcatgcgtga tgcagcagct aacgatgcag ttgtattgtt cgttggtact
    20881 aagaaacaag cagctgatgc agttgctgaa gaagcagtac gttcaggtca atacttcatc
    20941 aaccaccgtt ggttgggtgg aactcttaca aactggggaa caatccaaaa acgtatcgct
    21001 cgtttgaaag aaattaaacg tatggaagaa gatggaactt tcgaagttct tcctaagaaa
    21061 gaagttgcac ttcttaacaa acaacgtgcg cgtcttgaaa aattcttggg tggtatcgaa
    21121 gatatgcctc gtatcccaga tgtgatgtac gtagttgacc cacataaaga gcaaatcgct
    21181 gttaaagaag ctaaaaaatt gggaatccca gttgtagcga tggttgacac caatactgat
    21241 ccagatgata tcgatgtaat catcccagct aacgatgacg ctatccgtgc tgttaaattg
    21301 atcacagcta aattggctga cgctattatc gaaggacgtc aaggtgagga tgcagtagca
    21361 gttgaagcag aatttgcagc ttcagaaact caagcagatt caattgaaga aatcgttgaa
    21421 gttgtagaag gtgacaacgc ttaatttata caaatagtaa ttacctagga gggcggggct
    21481 tagcccggct ctcctatttt caaaaaatat aggagaatta aaatggcaga aattacagct
    21541 aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg ttatggacgc taaaaaagcg
    21601 cttgtagaaa cagacggtga catcgaaaaa gcgattgaat tgcttcgtga aaaaggtatg
    21661 gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag gtttgactgg tgtttatgtt
    21721 aacggtaatg ttgcagcagt tattgaagta aacgctgaaa ctgacttcgt tgcaaaaaac
    21781 gctcaattcg ttgaattggt aaatactaca gctaaagtca ttgctgaagg aaaacctgct
    21841 aataatgaag aagctcttgc tttgataatg ccttcaggtg aaactcttga agctgcatac
    21901 gtatctgcaa cagcaactat cggagagaaa atctcattcc gtcgctttgc attgattgaa
    21961 aaaacagacg cacaacactt tggagcatac caacataacg gtggacgtat cggtgttatt
    22021 tcagttgttg aaggtggaga cgaagcactt gctaaacaat tgtcaatgca catcgcagcg
    22081 atgaaaccaa cagttctttc ttacaaagaa ttggatgagc aattcgttaa agatgagttg
    22141 gcacaattga atcacgttat cgaccaagac aacgaaagcc gtgcaatggt taataaacca
    22201 gctcttccac acttgaagta tggatcaaaa gctcaattaa ctgatgatgt tattgctcaa
    22261 gctgaagctg acatcaaagc tgaattggct gcagaaggca aaccagaaaa aatctgggac
    22321 aaaattatcc caggtaaaat ggatcgcttc atgcttgata atactaaagt tgaccaagct
    22381 tacacacttc ttgcacaagt ttacatcatg gatgacagca agacagttga agcatacctt
    22441 gaatcagtta acgcttcggt agttgagttt gctcgctttg aagttggtga aggtatcgag
    22501 aaagctgcaa acgacttcga agctgaagtt gcagctacaa tggcagcagc cttgaataac
    22561 taataataga aaggaagcaa tttgcttcct tttttgtata gggcttatat ttttcctgaa
    22621 attgtttttc ccccagtctc actcgattag aataattaaa acaaaaagcc ctataataag
    22681 ggctaagtgc atttaggaga ctatacttca aattcatagt aagcgcgtca taacaaggta
    22741 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg gaggaaaggt
    22801 attgtactat gatgattcaa ctaagtgatt taggtcaagt tcacattgtt tgtggcaaga
    22861 cagatatgag gcagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
    22921 attctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc tttaaagtcc
    22981 tttactggga tggtcaagga ttttggctac tatataaacg ctttgagaac ggaaaactga
    23041 cttggctaag tacagaaaag gatgtcaaag ctctcacatc tgaacaagta gactggctta
    23101 tgaagggctt ttctatcact ccccaaatat agtggtttga aactagaata gtacacctct
    23161 gcttctaaaa cattgttaga aatcgatttg actgtcctgt tcttatttca ttttactata
    23221 aattcatcag aaagtcgtga tttctattga aatgaggact ttctttttat actcatctgt
    23281 tttcacaaag cattctagtc catctccgat taacgatgga ctttatcacc tccttctcca
    23341 gtccttgtat aacatcttgg agttgattca tgacatcttc caaaattt
//
LOCUS       DAVKWS010000028        22983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKWS010000028 DAVKWS010000000
VERSION     DAVKWS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.028"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(16..3192)
                     /gene="carB"
                     /locus_tag="VW852_001210"
     CDS             complement(16..3192)
                     /gene="carB"
                     /locus_tag="VW852_001210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV0272361.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTRLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAIAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKIAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3505..4584)
                     /gene="carA"
                     /locus_tag="VW852_001211"
     CDS             complement(3505..4584)
                     /gene="carA"
                     /locus_tag="VW852_001211"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV0272362.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4634..5557)
                     /locus_tag="VW852_001212"
     CDS             complement(4634..5557)
                     /locus_tag="VW852_001212"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV0272363.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEDLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5576..6097)
                     /gene="pyrR"
                     /locus_tag="VW852_001213"
     CDS             complement(5576..6097)
                     /gene="pyrR"
                     /locus_tag="VW852_001213"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV0272364.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6308..6937)
                     /gene="nth"
                     /locus_tag="VW852_001214"
     CDS             complement(6308..6937)
                     /gene="nth"
                     /locus_tag="VW852_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV0272365.1"
                     /translation="MVLSKKRARKVLEEIISLFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6937..7479)
                     /locus_tag="VW852_001215"
     CDS             complement(6937..7479)
                     /locus_tag="VW852_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV0272366.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7649..7965
                     /locus_tag="VW852_001216"
                     /pseudo
     CDS             7649..7965
                     /locus_tag="VW852_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
     gene            8204..9763
                     /locus_tag="VW852_001217"
     CDS             8204..9763
                     /locus_tag="VW852_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0272367.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQSVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9912..10811)
                     /gene="htpX"
                     /locus_tag="VW852_001218"
     CDS             complement(9912..10811)
                     /gene="htpX"
                     /locus_tag="VW852_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV0272368.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(10813..11373)
                     /locus_tag="VW852_001219"
     CDS             complement(10813..11373)
                     /locus_tag="VW852_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV0272369.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11467..12180
                     /gene="rsmG"
                     /locus_tag="VW852_001220"
     CDS             11467..12180
                     /gene="rsmG"
                     /locus_tag="VW852_001220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV0272370.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFECYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12434..13717
                     /locus_tag="VW852_001221"
     CDS             12434..13717
                     /locus_tag="VW852_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV0272371.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13912..15483)
                     /gene="ffh"
                     /locus_tag="VW852_001222"
     CDS             complement(13912..15483)
                     /gene="ffh"
                     /locus_tag="VW852_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV0272372.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15495..15827)
                     /locus_tag="VW852_001223"
     CDS             complement(15495..15827)
                     /locus_tag="VW852_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV0272373.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(15918..16295)
                     /locus_tag="VW852_001224"
     CDS             complement(15918..16295)
                     /locus_tag="VW852_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV0272374.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKRDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYILTPMGMQEFTIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16367..17671
                     /locus_tag="VW852_001225"
     CDS             16367..17671
                     /locus_tag="VW852_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV0272375.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            17684..18490
                     /gene="yidA"
                     /locus_tag="VW852_001226"
     CDS             17684..18490
                     /gene="yidA"
                     /locus_tag="VW852_001226"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV0272376.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(18546..18617)
                     /locus_tag="VW852_001227"
     tRNA            complement(18546..18617)
                     /locus_tag="VW852_001227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18582..18584),aa:Arg,seq:cct)
     gene            complement(18660..19007)
                     /gene="rplS"
                     /locus_tag="VW852_001228"
     CDS             complement(18660..19007)
                     /gene="rplS"
                     /locus_tag="VW852_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV0272377.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19126..19455)
                     /gene="crcB"
                     /locus_tag="VW852_001229"
     CDS             complement(19126..19455)
                     /gene="crcB"
                     /locus_tag="VW852_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0272378.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(19449..19823)
                     /gene="crcB"
                     /locus_tag="VW852_001230"
     CDS             complement(19449..19823)
                     /gene="crcB"
                     /locus_tag="VW852_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0272379.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19820..20086)
                     /locus_tag="VW852_001231"
     CDS             complement(19820..20086)
                     /locus_tag="VW852_001231"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV0272380.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20202..20645)
                     /locus_tag="VW852_001232"
     CDS             complement(20202..20645)
                     /locus_tag="VW852_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV0272381.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            20749..21684
                     /locus_tag="VW852_001233"
     CDS             20749..21684
                     /locus_tag="VW852_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV0272382.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21738..22022
                     /locus_tag="VW852_001234"
     CDS             21738..22022
                     /locus_tag="VW852_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV0272383.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(22079..22297)
                     /locus_tag="VW852_001235"
     CDS             complement(22079..22297)
                     /locus_tag="VW852_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272384.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(22294..22868)
                     /locus_tag="VW852_001236"
                     /pseudo
     CDS             complement(22294..22868)
                     /locus_tag="VW852_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6769 a         5138 c         4395 g         6681 t
ORIGIN      
        1 tcttattttt tctatttaaa tcgcttctgt aacaaagcta cgactttcaa gtaccttaag
       61 catggcatta gctgtatcta gcgctgtgaa gaggggcacc ccgtgttcaa tggctgaacg
      121 acgaatttgc tcaccatctt cgtcagcagt tcgttttgtt cctactgtgt taatgatagc
      181 ttgaattctt cctttgcgta caaaacttgg gatatcctta tcgtcatcac caatcttacc
      241 aacaggttgg gcttgcaagc catgactagc aaagaaggct gctgtcccct ctgtcgcaag
      301 gattccataa ccaatgtttt ggaaacgacg agccaagttc aaggcttctt ctttggcatc
      361 atcagcgatg gtaaagacga cattaccaaa agttggcaag tgtagataag aagcttcaaa
      421 ggctttatag agagcttttt ccaaagtagc atcagaaccc ataacttcac ctgttgactt
      481 catttcagga ccgagcaagc tgtctacctt agctagtttg gtaaaggaga agacaggtgc
      541 cttgatatga acacgggtgc tttcagggta aagtccattt tggtagccaa gttctgataa
      601 actttgacca agaatgagct tggtcgctac ttgagccata ggaatattgg ttaccttaga
      661 taggaatgga acggtacggc tggcacgtgg attgacctca ataacgtaga ctttttcatc
      721 cttgataaca aactggatgt tcatcattcc aaggcagtga agaccgattg ctaagcgttt
      781 ggtgtagtct gcgatggtct cctgaacctt ttgcgacaag gtttgtggtg ggtaaacagc
      841 cattgagtca cctgagtgga caccagcacg ttcgatatgc tccataatac caggaatgag
      901 tacattttta ccatctgaaa tggcatcaac ttcgcactct tgcccaacga tgtaagagtc
      961 gacaagaact gggtggtctg gactagcctt aacagcagtt cgcatgtaag aacgaaggtc
     1021 ttcttcgttt tcaacgattt ccatggcacg tccaccaagt acataagatg ggcggacaag
     1081 aactgggaag ccaatcttac gagctgcaag agctgcttct tcttcattgg tagccgtttg
     1141 tcctggtggc tgtggaatat ccaattcttt gagagcttgc tcgaagaggt cacggtcttc
     1201 ggcacggtct aggtcagcaa cctgtgtacc aaggatggtc acacctgctt ttgccaatgg
     1261 ctccgcaagg ttgatggctg tttgaccacc gaactgaacg ataactccct ttggttgttc
     1321 caagtcaatg acgttcataa catcttcgaa tgtcaatggc tcaaagtaaa gcttatctga
     1381 tacagagaag tctgttgaaa cggtctctgg gtttgagttc atgataatag cttcataacc
     1441 agctgcctgg atagccttaa cagagtgaac ggttgcgtag tcaaactcaa ccccttgacc
     1501 gatacggatt ggacctgaac ctagaacaag tacagattct ttatcagatc tgatagattc
     1561 attttcccaa ccataggttg aatagaaata tggcgtttca gagtcgaact ctgccgcaca
     1621 agtatctacc atcttataga ctgggacaat cttgttttcc aaacgaagtt ggcgaacttg
     1681 atcatcagtc gttccccaga gttcagcaat cttacggtct gagaaaccat taagtttggc
     1741 tattttcaaa acttctagat cttgtggatg agcacccaat tcttgctcaa tttcaaagat
     1801 atgcaagagt ttatcaagat agaagatatc aatttttgta agctctgcaa tttcttcagg
     1861 tgtgtagcca cgacgaattg cttctgatac gtagaagaga cggtcatctt gggctttgac
     1921 aaccttttca atcaaggtat catcagaaac tgctgcaagt tcaggtattt cattgtggtg
     1981 caccccaatt tcaagggatc ggcaggcctt gagaagagat tcctcgatgt tacgaccgat
     2041 tgccatgact tctccagtcg ccttcatttg tgtaccgaga cggcgttcac ccttttcaaa
     2101 cttgtcaaat gggaaacgtg gaatcttagc aactacgtag tcaagggctg gttcaaacat
     2161 ggcataggtt gaacctgtaa ctgggtttat aacctcatcc aaggtcaaac ctactgcaat
     2221 cttggcagcc aacttagcaa tcggatatcc tgtcgcttta gaagcaaggg ctgacgaacg
     2281 tgatacacga gggtttactt cgataacata atacttgaaa ctgttaggat caagagctag
     2341 ctgaacatta catccacctt caatcttgag ggcacgaata atgctcaagc tcgcatcacg
     2401 aagcatttgg ttttcatagt ctgacatggt ttgcgcaggg gcaaatacaa tggaatcccc
     2461 tgtgtgaatc ccaactgggt caaagttttc catgttacaa acaaccaagg cattgtcagc
     2521 tgagtcacgc atcacttcgt attcaatttc cttgaaaccg gcaatcgaac gttcaatcaa
     2581 acattgggta acaggtgaca atttcaaacc attttcagtg atttcacgca attctttctc
     2641 gttggcacac ataccaccac cagtaccacc aagggtaaag gctggacgaa cgatgactgg
     2701 gtagccaatt gtcgctgcaa aggcaattgc ttcttctact gtgttaacaa tttcagattc
     2761 tggaatgggt tgctcaagct cttccatcaa ttgtttaaag aggtcacggt cctccgcttg
     2821 gtcaatggca gacaatctag taccgagaag ctctacacca agctcatcta agatgccatt
     2881 tttagacaat tccatggcca tgttgagacc tgtctgacca ccgagtgttg gtagcaaggc
     2941 atctggacgt tccttacgaa gaatacgtgt cacaaactca agtgtaatcg gttcaatgta
     3001 aaccttatca gcaatttcct tgtccgtcat gatggttgca ggatttgagt taaccaaaac
     3061 aacctcataa ccttcctctt tcaacgacaa gcaagcctgg gtcccagcgt agtcaaactc
     3121 agcagcctga ccaataataa tcggaccaga accaatcacc ataatttttt gaatatcagt
     3181 acgtttaggc atatataaga tattaagggc gtcaagcgga caaagctaaa ataggagtta
     3241 tgacgaagaa ctgtcagttc taggaataac tatcttttta gcaccgtccg tagcccgtat
     3301 tcagttcagc aaatacggag cacccttctc ctttctattc gtcgcctctc agggcgacat
     3361 taaataagat acaaaggacg aatagaaagc gattgaattt tagaaaatca aggaaggagt
     3421 gacaatccaa gttggtttct ctacattctg agctttccgt ccgtgttcag ttacataaat
     3481 tctccgacga gcttttactc gttcttagtt tgattgttta aaaacttcca tcatctcgat
     3541 aaactcgtca aataggtagc tagcgtcgtg tggcccaggg gctgcatctg ggtggtattg
     3601 aacagagaaa gcaggttggt atctgtggcg cacaccttcc actgacttgt cattgatttc
     3661 ttcgtgggta ataatcaagt gctctggcaa atcctcgcgg ctgactgcat aaccatggtt
     3721 ctggctggtg aagtctactc gtcctgttgc gatttcacgt accgcatggt tgaatccacg
     3781 gtgaccaaac ttcatcttat aggtcttagc cccgtttgcc attgcaaaga gttggtgtcc
     3841 catacaaata ccaaagattg gaatttttcc ttgtacaccg cgaatcatgt cgagtgcttg
     3901 tggaacgtct tctgggttac ctggaccatt tgacaacata actccgtcag gattgagatg
     3961 gagaatttct tcagccgttg tcgaataagg aacaactgtc acgttacagt tgcgtttaga
     4021 aagttcacgt aggattgagt gcttgagacc aaagtccact agcaccacgc tcaaaccaac
     4081 tcctggagct ggataagacg ttttagtaga aacctgtttg atattgtctg tcggtaaaac
     4141 tgttgcttgg agctggtccg tcacatggtc catactgtcc ccaacatggg tcaaggttgc
     4201 acgcatagta ccatgcttac ggataatctt ggtaagagca cgcgtatcaa ttcctgaaat
     4261 ccctggaatt ttcttggctt tcaaaaattc atccaaggtc atttggttgc gccagttgct
     4321 agctctacgc gcttcttcaa aaacaacgac tcccttacaa gttggaataa tggattcata
     4381 atcatcacga ttaataccat aatttcctac caaaggataa gtaaaggtca agatttgtcc
     4441 attataagac tggtctgtaa tggattcttg gtagccggtc attcctgtat taaagacgat
     4501 ttcgcctgtt acatcaatat ctgctccgaa ggccttgcct tcaaaaactg tgccatcttc
     4561 taatactaga attctttttg tcatattttc acctctcgtg gacgctcact ggcgtctttt
     4621 aacgtcttgt gttttagttg gcgtttctac tcgctagtac ggattctaag attgccattc
     4681 gaacaaagac accattggtc atttgttgga caatccgtga ttttggtgct tcaaccaagt
     4741 ggtctgctat ttctacatca cgattgattg gagctgggtg catgaggatt gctgtttctt
     4801 tcaaacgatc gtaacgttct tgagtcaagc catgttgggc atggtagtct tcttttgaaa
     4861 atacagctcc actatcatgg cgttcgtgtt gcacacggag aaacatcatg acatcaacct
     4921 gatcaatgat ttcatcaatg gttacaaact ttccatagtc tgcaaactct tgacttctcc
     4981 attcctcagg tccagcgaaa aagagttcag ctcccaagcg tttcaaaatc tgcatattgg
     5041 atttggcaac gcgtgagtgg tccaagtcac ctgcaatagc aactttaaga tcctcaaagt
     5101 ggccaaattc ctcataaatg gtcatcaaat caagcaagct ctggctaggg tgttggcccg
     5161 aaccatctcc accattgatg atggaagtcg taatcgttgg actagcaatc aattctctat
     5221 agtagtcgac ctctggatgg cgaatcacac agacatccac tcctaaagca gacagagtca
     5281 aaatggtgtc ataaagtgtc tcacccttat taaccgaact agtcttcaca tcaaagtcaa
     5341 gtcgttccaa tccaagttta atctctgcga cttcaaagga cttatgtgtc cgtgtagaat
     5401 cctcaaagaa gagattggaa acaatcggat ggtcttcata gggaagctgg gctccatttt
     5461 taaactcaat tcctcgcttg atcaatttca ttacttgatc gacagtgagg tcttccatgg
     5521 acaccacatg gttcaatgct tgttgatttt ctgacatggc tactccttta actttctaag
     5581 cttcttcagt aatcagaact ctgtcttggt catcaagttc tgtcatctct acgatgattt
     5641 cttcagaacg actggttggg atattttttc caacgtaatc tggacggatt ggcaattctc
     5701 tatgtccacg atcgactaga actgctaaac tcacacgcgc aggacgacca tgaccgacaa
     5761 tattatcaat agcagcacgg atggtacgac ctgtatagag cacatcatcc accaagataa
     5821 cttcgcggtc tgtcacatcg acagaaacca aagaagtatc ttctccactt ttaacatcat
     5881 cacggaaagg tttagtatcc aattccacaa caggaactga aagattttct aactgcttca
     5941 aacgttcttg gattcggtgg gcaataaaga caccacgagt tttaatacca gccaagacga
     6001 tcttattcaa atctttgttg cgttcgataa tctcataagt aatacgcgta atcgctcgtt
     6061 tgacggtcaa ttcgtctaca acttcttttg ttttcatgac aaacctccaa aaagaaaagt
     6121 ctccttaaac aaggagactt gaaatttata gccaagcgag ccctactgca cacagtatag
     6181 acttcaccct tctactttat cgcgctcctt gcctgcctca cgggacaggt ttaaaggaat
     6241 atttagttat catttactat agcacaaagc atgcttaaaa tcaagcaaaa agtttcaatg
     6301 tagtatctta caaattgcta aaatcatata attgtgggta ctggtcacac tctggatttt
     6361 ttggatggca aatggctctt ccaaaataaa tcatggcctg atgggcagct aaccactgct
     6421 caggcggcaa gatatccatg acccgctttt ccacctcaag tggcgtcgct gattttttga
     6481 caatatcgtg gtgtttgcaa atacgctcca catgagtatc cactgcaaag gctggaatcc
     6541 caaatcctac actcatgaca acattagctg tcttgcgacc aacacctgcc aaactctcca
     6601 attcttcacg tgtctgaggg acttgaccat caaaatcgtc tagtaactgt tgggcacatt
     6661 ttttaaggaa tttagcttta ttccgataca atcccaagcg agaaatatgt gaagcaatct
     6721 cactctctgt cgctacagac atagcttggg gtgttggaaa ggcaacaaag agacctggtg
     6781 tggccttatt taccgctgca tctgtcgtct gggctgataa catgaccgca accaggagtt
     6841 caaaatgatt ggtaaaatca agactaggct tggcatctgg gaagagggaa atgatttctt
     6901 ctagcacctt tcgtgctcgt ttttttgaca agaccattat tcatctccgt caaatagtcc
     6961 ttgtaagcca gcaaaaggac tgttttcttc tttctttact gcttgttgag cttggtattc
     7021 ttcctctgtc atgatttgcc agtcatttcc tgagacaaat ccttgaccag cttcttcttc
     7081 agccgtcaag accttgatag gaatgtttag caggatattg tctgatacac tctcagcaag
     7141 gtcaagctcc ccattttcga tgggcaagac caagtcatca tctaaaactt cttgatctag
     7201 ctggttagtt gcgccttcca tgaaaacttc cgtgactgga taagattcaa ctaactcaac
     7261 tggctccata ctgcgactcg aagcaagaac aatggtataa gatagttgat aatctaagaa
     7321 atacatacgg tcttcatatt gtactttccc aactgcaagg atatctttta catctaaaat
     7381 ttcttgatta cgtgcacgca ggtcatcaac taaatctaac gtttgttcaa agttcaaacc
     7441 ttcagactgc ttacgaattt cttgaatatt taatttcata cttcctccat aaagatttac
     7501 tctcttgatt ataccatgaa aaggctacaa atcagcacac caaactttgt aattaaaatt
     7561 cgaaatttta acatattcac tatgacagtt ttatttttta gtgctatact atagggaaag
     7621 agtacatcag atcaaggagg atgctcacat ggaagacaag aaactcattc aactcctatc
     7681 caagttaaat aaaagctacc aaaactgtaa acagggtacg gcagatgata ttcgactaca
     7741 agagctgcta aacactacta tgcaagagct caaaaaacgg aaaagttgaa caacagtatc
     7801 ttaattgatc ttgagaaatt ttaccaaccc accagtcttc tgattggact gggtagccta
     7861 aaactaaacg atcaagcacg cactgcttgg cgaaactatg ataaattcca ttacgagcat
     7921 gtcaaacacg tactaagtct ctatggacct gtttttggat tttagagcat agaatttcca
     7981 gttttctgtt gacaaaattt ccttaaaggt ataatataaa gatactaata ctcggaggta
     8041 agggagacat gaacaactaa gtctatcaaa taaagaacct ttatttagta gatcttgttt
     8101 ttgtctcttt ttgtgtgctc ttttatgctc tttttctggc atgttaatag agtttttttg
     8161 acatagactt tgggctctac taggtaaagt agagcttttt gttatgcact atgaacattc
     8221 tagaaaggga aatcatatga taaaaatcaa tcatctaacc atcacacaaa acaaagattt
     8281 acgagatctt gtatctgacc taagcatgac catccaagac ggggaaaagg ttgctattat
     8341 tggtgaagaa ggaaatggca aatcaacctt acttaaaatt ttaatggggg aagctttgtc
     8401 tgatttcact atcaagggaa acatccaatc tgactatcag tcactggcct acattcctca
     8461 aaaacttccc gaggagctga aaaagaaaac tttacacgac tacttctttt tagattctat
     8521 tgatttagac tacagtatcc tctatcgttt ggcggaggaa ttgcattttg atagcaatcg
     8581 tttcgcaagt gaccaaaaga ttggcaatct atcagggggc gaagctttga aaattcagct
     8641 tatccatgag ttagccaaac cctttgagat tctattttta gatgaacctt caaatgacct
     8701 agaccttgag acagttgatt ggctaaaagg ccagattcaa aagaccaggc aaaccgttat
     8761 tttcatttcc catgatgaag actttctttc tgaaacggca gacactattg ttcacttgcg
     8821 actggtcaaa caccgtaaag aagcggaaac gctagtagag catttagact atgatagcta
     8881 tagtgagcag agaaaggcta attttgccaa acaaagtcag caagctgcta acaaccaaag
     8941 agcctacgat aaaaccatgg aaaaacatcg gagagtcaag caaagtgtag aaactgcgct
     9001 tcgagctacc aaagatagta ctgccggtcg cctattggct aaaaagatga aaactgtcct
     9061 ctcacaagaa aaacgctacg aaaaggcagc tcagtccatg actcaaaagc cacttgaaga
     9121 ggaacaaatc caacttttct tttcagacat ccaaccatta ccagcttcta aagtcttagt
     9181 ccaactggaa aaagaaaatt tgtccattga cgaccgagtt ttggttcaaa aactacaact
     9241 aactgtccgt ggccaagaaa aaatcggtat tatcgggcca aatggtgttg ggaaatcaac
     9301 tctgttagcc aagttacaga gacttctgaa tgataaaaga gagatttcac ttggttttat
     9361 gccacaagat taccacaaaa aactgcaatt ggatttatcc ccaatagcct atctcagtaa
     9421 aactggggaa aaagaggaac tacagaaaat ccaatctcac ctagctagtc tcaatttcag
     9481 ttatccagaa atgcagcatc aaattcgctc cttatctggc ggacaacagg gaaaactcct
     9541 gcttttggat ttagtcctgc gcaaaccaaa ctttctcctg ctggatgaac ccacacgaaa
     9601 cttttctccc acttctcaac cccaaatcag aaaactcttt gccacttatc ctggcggtct
     9661 catcactgtt tcgcatgacc gtcgtttctt aaaagaagtc tgctcgatca tctatcgcat
     9721 gacagaacac ggtttgaagc tagttaattt agaagattta taaatttgca acatagcaga
     9781 taggtggggc aaaaacacaa ttttaggaga aaaagaagta aatcttccca tagtaaaacg
     9841 cataatatca agttttcaca cctgatatta tgcgtttttc agattttaaa gacttttttc
     9901 cagccttcat tttacatatg ttttaaacgt tcaatccgtt ctgagatagg tgggtgggta
     9961 taaaagagtt tttggaaccc cccacctttc ttgggatcat tgatataaag ggcactgcta
    10021 gcatcatcga cgtggcgact cataggtttg ctattgtcca acttatctag ggcattaatc
    10081 attccctggg gattgcgagt cagctcgaca ctagatgcat ctgccagaaa ttccctctga
    10141 cgagaaatag cgagctgaac caaggttgca gcgagaggtg ccagtacaat agctagtagg
    10201 gaaaccacta gcataatgat ttcaagacca tttccatctc ggtcatcatc acttcgtctg
    10261 cgacctgctc caccccacca catcatacga cctgccatac tagaaagcat ggtgatagca
    10321 ctagcaaggg caactgcaat agtcgaaata cggatatcat aattacgaat atgactgact
    10381 tcatgtccca taacagcttc tagttcttca cgattcatga tagctagtag acctgaagtc
    10441 gcagcaacag ccgcattttg aggattagaa cctgtcgcaa aggcatttaa ggctggatca
    10501 tcaatgatga aaacacgggg cataggaatc tgagcgacca gagccatatc ttccactaca
    10561 tggtagaggt ctggtgccgt ttgctcatcc acctcacgcg ctccattcat ggacatgaca
    10621 atctctgtcg attgaaaaat catagacaaa gcgtagataa agccgataat cagtgcgata
    10681 accaaaccac caagtccaga tcttataaag agataaccaa ccgcataacc aacaagagct
    10741 aagagtagga aaaataccag caacaaaatc caggtttttc gtttattgct tgcaatttga
    10801 tcaaacaaca tcttagtcac ctaaaccgct aaaatcaact ttaggaaccg acttttcctc
    10861 ttcaggtgtt tgaaggaaat ctgccgcttt aaatccaaac attccagcga taatattgct
    10921 cgggaaagtt tctaatttta cattgtagtt gctgacaaca ctgttataga gttgacgaga
    10981 gtaagaaatt ttattttctg tgtttgtcaa ctcctcttgc aatttaacaa agttagcact
    11041 agctttcaaa tctggatagc tttctgcaac tgcaaaaata cctgaaacct gacgagtgag
    11101 ggcatcactg gctttcatag cttctgctgg tgaagtcgct gccgccactt ggttacgtag
    11161 ttctgccacc ttttcaaggg tagaaccttc atatttggca taacctttta cagtctcaat
    11221 caagtttggc aagaggtcat ttcgacgttt caactgaaca tcaatctgac tccaagcctc
    11281 cttggtttgc atacgatttt taaccaaacc gttatagcta acaatcacaa aaataacaat
    11341 aagagcgata actccaagaa taatccaagt catgatataa gtcctttcta cttttagatt
    11401 agtaccagta tatcaaattt ttaatgattg tggtaaaata agatgatact aaagaaggaa
    11461 ataactatga aaccaaaaac attttacaac ttgcttgccg agcagaatct tccactttcg
    11521 gaccagcaaa aagaacaatt tgaatgttat tttgagctct tggtcgagtg gaatgagaag
    11581 attaatttga cggcgattac ggacaaggaa gaagtttatc tcaaacattt ttacgattcg
    11641 attgcaccca ttcttcaagg tttgattccc aatgaaacta tcaaacttct tgatatcgga
    11701 gctggggcag gatttcctag tctaccaatg aaaattctct atccggagtt agatgtgacc
    11761 attattgatt cactcaataa gcgcatcaac ttcctacaac tcttggctca agaactggat
    11821 ttgaacggag ttcatttcta ccacggacgt gccgaagatt ttgcccaaga caagaacttc
    11881 cgtgctcaat atgattttgt aacagctcgt gcggttgccc gtatgcaggt cctatctgaa
    11941 ttgactattc cctaccttaa ggttggtgga aaactattgg cactcaaggc cagcaatgcg
    12001 cctgaggagt tgttagaagc taagaatgcc ctcaatcttc tttttagtaa agtcgaagac
    12061 aatctcagct acgccctacc aaatagagat ccgcgttaca tcacagtggt agaaaagaaa
    12121 aaagaaacac caaataaata tccacgtaag gctggtatgc caaataaacg cccgctttaa
    12181 atttttaagt aaaaaaatat ttacaaaatc agcctcgctc ttttatttct aggctcggga
    12241 aaaaatgatt tacaaaatca tttttttctg ctatactatc ctaagcaaag gtttttaatg
    12301 tcatcccgtg aggtgacgaa gacgcaaaaa tatttgaaac tctttaaagt ctagatttta
    12361 gagaagtctt actctgaggg cctattgctg taaaataatg ggctcttttc tgatgcccaa
    12421 aagtgaggtt tatatgaaac aagaatcaac tgttgatttg ttactagacg ttgaccaacg
    12481 tccttcggat ggaaaaggaa ttctccttag cttccaacac gttttcgcca tgtttggtgc
    12541 gaccatcttg gtaccattga ttttgggaat gcctgtatct gttgcccttt ttgcttcagg
    12601 tgttggaaca ctcatctaca tgattgctac tggttttaaa gttccagttt atctaggttc
    12661 ttcatttgcc tttatcacag ctatgtcact ggctatgaaa gaaatggggg gtgatgtatc
    12721 tgctgcccaa acaggggtta tcttgactgg tttggtctat gtccttgttg ctaccagcat
    12781 ccgatttgta ggaacaaaat ggattgataa actcttgcca ccaatcatta tcggtcctat
    12841 gatcatcgtt atcggtcttg gacttgcagg ttcagctgtt accaatgcag gtcttgtagc
    12901 agacggaaat tggaaaaatg ctctggtagc cgttgttact ttcctaattg ctgcctttat
    12961 caatacaaaa ggaaaaggct tcctacgaat cattccattc ctctttgcca ttatcggtgg
    13021 ttaccttttc gcactaactc ttggcttggt tgactttaca ccagttctta aagccaactg
    13081 gttcgaaatt cctggtttct acttgccatt tagcacaggt ggtgccttta aagagtacaa
    13141 tctttacttt ggtccagaag ccatcgctat cttgccaatc gctatcgtaa caatttctga
    13201 acatatcgga gaccatactg ttttgggtca aatctgtggc cgtcaattct taaaagaacc
    13261 aggtcttcac cgtactctta ttggtgacgg tatcgcaact tctgtttctg ccttccttgg
    13321 tggaccagcc aatacaactt acggagaaaa tacaggggtt atcggtatga ctcgtatcgc
    13381 ttctgtctca gttatccgta acgctgcctt catcgcgatt gccctcagct tccttggtaa
    13441 attcactgcc ttgatttcaa ctattccaaa cgctgtactt ggtggtatgt caatccttct
    13501 ctatggggtt atcgccagca atggtttgaa agtcttgatt aaagaacgtg ttgatttcgc
    13561 tcaaatgcga aacctcatca tcgcaagtgc tatgttggtt cttggacttg gaggagctat
    13621 ccttaaactt ggtccagtta cactttcagg tactgccctt tcagccatga caggaatcat
    13681 cttgaacttg atcttgccat acgaaaataa agactaagag tctaaataca cctaatccac
    13741 tcagacagct gagtggattt ttcgcatacc ataataaaag tgtcttaaca aaattattaa
    13801 aatcaaaaaa cgtataatat cagatattct aaaaccttga tactgtacgt tttatcatag
    13861 aaatttttac tttattttct catcaaatga gatttgcatc aatctcttgt cttacttgcg
    13921 tttcttcttc gctttcttca ttttgttagc catacgtttc atggactgtt tcatggcaaa
    13981 ttcaccaatt ttacctttca aaccgccacc aaacatctgg ctcatatctg gcattcctgc
    14041 tcctccgaga gctgataagt caggcatacc gccttgtccc atcattcctt caagggcaga
    14101 catatccatt cctcccatat ttggcatatt tttaggaagg ttatttggat taatccccat
    14161 ttgcttcatc attttattca tatccccaga cataacaccc tgcatgagct gtttagcctg
    14221 gttaaagtcc ttgatgaatt tattgacttc gacgaatgta tttccagaac cagcagcaat
    14281 acgacggcga cggcttggat ttaacaaatc tgggttttca cgctcttcag gtgtcatcga
    14341 agacacaatg gcacgtttac gagcaatctg gcgttcatcc accttcatgt tttgaagggc
    14401 tggattgttg gccatacctg gaatcatctt gagcaagtct tccatcggcc ccatattttg
    14461 cacctgatct aattgatcga tgaaatcatt aaaatcaaag gtgttttcgc gcatcttctc
    14521 agccatttca agggcttttt gttcatcgta ttcctgagaa gctttctcaa tcaaagtgag
    14581 catatccccc ataccaagga tacggctaga catgcggtct gggtggaagg tttcaatgtc
    14641 cgtaatcttt tcacctgtac cagtgaactt gattggtttt ccagtaatgt gacgaacaga
    14701 cagagcagca ccaccacgag tatcgccatc aatcttggta aggatgaccc cagtcacttc
    14761 caactgagca ttaaactcac gcgcaacatt ggctgcttcc tgaccaatca tagcatcaac
    14821 gacaagcaag atttcatttg gttgagccaa tgctttcaca tcacgaagct cattcatgag
    14881 gagctcatca atctgcaaac gacccgcagt atcaatcaag acatagtcgt tatgattagt
    14941 ttgggcttgc tccaaacctt gacgtacaat ctcaacagct ggtacttctg ttccaagtgc
    15001 aaagacaggc acatcaatct gttgtcccaa ggtcttaagc tggtcaatgg cagctggacg
    15061 ataaatatcc gccgcaatca tcaaaggacg agcattttct tctttcttga gtttgttggc
    15121 caatttacca gcaaaggttg ttttaccagc cccttgtaaa ccaaccatca tgatgatggt
    15181 tggaatctta ggtgacttga taatttctgc cgtatcagaa cctaaaacgg ctgtcagttc
    15241 ttcatcaacg attttaataa tctgttgcgc aggattaagc gtatcaatga cctcatgccc
    15301 gactgcacgc tcacgaactt tcttgataaa gtcctttaca acaggcaagg caacgtcggc
    15361 ctcgagcaag gccaagcgaa tttctttggt tgcctcttgg acatcagatt cagagatttt
    15421 tcctttttta cgtagatttt taaagacgtt ctgcaaacgt tctgttaaac tttcaaatgc
    15481 catttttctt cctcttattc tctattatca atgcttgtta aaatttctat ctgctcctgc
    15541 agaaagtcat ccttgggata gcgctccaaa atctgatcaa aaatctgact gcggacaata
    15601 tagtccgagt acatgtgcaa tttcatctca taatcttcca gaatcttttc tgttcgcttg
    15661 atattgtcat agacagcctg acgactgaca ccgaactcct cggcaatttc agcaaggctg
    15721 taatcatcag cgtagtagag ctcgatataa ttcatttgct tatctgtcaa aagcgccgca
    15781 taaaattcaa agagcgcatt catacgattg gttttttcga tttccataac ttttattata
    15841 ccaaaaatta gcctaatcta ccacactagg aagccgatcc aagaagatag atagctaaat
    15901 ttgaaaaaga catgagccta gccccaagta atttccaatt gatagctggc aaagggatgt
    15961 ccctcttgat tttgtagttg ataatctagt tcaatctttt gcctatcaac ttgataatgg
    16021 ctcgtttgga tggtaaactc ctgcatgccc ataggtgtaa gaatataggc taaactatcg
    16081 ctatccttta gaaagcgcat aatggtcttg ggattagaaa atcggctcat cacaagttct
    16141 tgaccatgaa atttaatcac tactttttcc ttttcctcat tatagaaaag caggtagcta
    16201 taatctcttt tttcatgcac ttccacatca taaagctggt caatcacttc caactgctca
    16261 tcaaactgaa tcgtatttcg catccgaatc ttcacatcag gccctctttc ttgtctcttg
    16321 tcctactatt ttaccaaaaa gagcaggatt ttgctataat ggtcatatga acgaaaaagt
    16381 attccgtgac ccagttcaca actacatcca tgtcaataat caaatcatct atgacttgat
    16441 taatacaaaa gaatttcagc gtttgcgccg gatcaaacaa ctgggaactt ccagttatac
    16501 cttccacggt ggagaacaca gtcgcttctc tcactgtcta ggagtctatg aaattgcacg
    16561 acgcatcaca gagattttcg aagaaaaata tcctgaggaa tggaatcctg ccgagtctct
    16621 cttgaccatg atcgctgctc tcctacatga ccttgggcat ggcgcctact cccatacttt
    16681 tgaacatctc tttgatacag accatgaagc tattactcag gagattattc aaagtcctga
    16741 gacagagatt caccaagtct tgctacaagt agcgcccgat tttccagaga aagttgccag
    16801 tgtcatagac catacctatc ctaacaagca ggtcgtacaa ctcatttcta gtcagattga
    16861 cgcagatcgc atggactatc tcttgcgtga ctcctatttt acaggagcat cctatgggga
    16921 atttgacctg actcgaatcc tccgagtcat tcgtcctatc gaaaatggta tcgcctttca
    16981 gcgcaatggc atgcacgcca tcgaagacta tgtcctcagt cgctaccaga tgtatatgca
    17041 ggtctatttc cacccagcaa cacgcgctat ggaagttctc ctacagaatc ttctcaagcg
    17101 cgctaaggaa ctctacccta aggacaaaga cttctttgca cgaacttccc cacacctcct
    17161 gcctttcttt gaaaaaaatg tgaccttgtc tgactatctg gctctagatg atggtgtgat
    17221 gaatacctac ttccagctct ggatgaccag tcctgacaaa attctcgcag atttatcgca
    17281 acgctttgtc aaccgcaagg tctttaaatc cattaccttt tcacaagaag atcaagacca
    17341 actcgctagc atgagaaaat tggttgagga cattggcttt gatcccgact actatactgc
    17401 cattcataag aactttgacc tcccttatga tatctatcgt cccgaatctg aaaatccacg
    17461 gacacagatt gagattttac aaaagaatgg agaactggcc gaactctcta gcctgtctcc
    17521 tatcgtccaa tcccttgctg gtagtcgtca cggagataat cgtttctatt ttccgaaaga
    17581 aatgttagat caaaacagta tctttgctag cattacccag caatttttat acttgattga
    17641 gaacgatcat tttaccccaa ataaaaacta gaagaggaaa tttatgagta ttaaactaat
    17701 tgccgttgat atcgacggaa cccttgtcaa cagccaaaag gaaatcactc ctgaagtttt
    17761 ttctgccatc caagatgcca aagaagctgg tgtcaaagtc gtgattgcaa ctggccgccc
    17821 tatcgcaggc gttgccaaac ttctagacga cttgcagttg agagacgagg gggactatgt
    17881 ggtaaccttc aacggtgccc ttgtccaaga aactgctaca ggacatgaga ttatcagcga
    17941 atccttgact tatgaggatt atctagatat ggaattcctc agtcgcaagc tcggtgtcca
    18001 catgcatgcc attaccaagg acggtatcta tactgcaaat cgcaatatcg gaaaatacac
    18061 tgtacacgaa tcaaccctcg tcagcatgcc tatcttctac cgtacccctg aagaaatggc
    18121 tggcaaagaa attgttaaat gtatgtttat cgatgaacca gaaattctcg atgctgcgat
    18181 tgaaaaaatt ccagcagaat tttacgagcg ctactccatc aacaaatctg ctcctttcta
    18241 cctcgaactc cttaaaaaga atgtagacaa gggttcagcc attactcact tggctgaaaa
    18301 actcggattg accaaagatg aaaccatggc aatcggtgac gaagaaaatg accgtgccat
    18361 gctggaagtc gttggaaacc ccgttgtcat ggaaaatgga aatccagaaa tcaaaaaaat
    18421 cgccaaatac atcactaaaa caaatgacga atccggcgtt gcccacgcca tccgaacatg
    18481 ggtactgtaa aaggaaagaa tagactaaaa ccgctcagct gagcggtttt tgatgtctaa
    18541 ttacatgccc cctgcaggaa tcgaacctgc aactactcct taggagggag ttgttatatc
    18601 cattgaacta agggagctag ataaaaactc tgctgaaaaa gcagagtttt ttagtcgaat
    18661 taacgacgga tttctttgat acgagctgcc ttaccttgaa gagcacgcaa gtagtacaat
    18721 ttcgcacgac gtactttacc gtaacgtaca acttcgattt tttcaacacg tggagtgtgg
    18781 attgggaaga tacgctcaac acctacaccg ttagagattt tacgaactgt gtagttttct
    18841 gagattccag cacctttacg tgcgataaca acaccttcaa aaatctggat acgttcacgg
    18901 ttaccttcga caactttcgc atgtacacga acagtgtcac caggacggaa tgatgggata
    18961 tctgtacgaa gttgaccttc agtcaagctt tggattaatg gattcatttt attctcctat
    19021 ctttgtcaat cttgaggaat cttcctcagc ggataaactg tatttttgtg cgtccattac
    19081 acacaagata tagtttacca aatttctaaa aaaaagtaaa gaaatttata gcaaaattcc
    19141 taaaaaaatc gcaaccaaac cacctatgta agtcaaagtc aggtaagaat aaaagacctt
    19201 cttatcactt agcagtcttt gaagttcgtc attcaaggtc gaaaaagttg ttaaaccgcc
    19261 acaaaatcct gttgctagaa tagcatatac ttccttagac tctacatgat tgtaaaatac
    19321 tccaattaaa aaacaaccta aaagattagc tatgagcgtt ccgagtggca atttagaagc
    19381 ttgattatag cgagaaaaga aataccgtac gagtgctccc aagccacagg cgattgcaag
    19441 atagacgatt accatttctt cctccctaga aaataagcta agagtaggcc tccaccgatg
    19501 ctcaaaagca aatacaggac taaactaaaa taacgccctg tatcaagcag cttcacagta
    19561 tcaagcatta ggctggaaaa agttgttaaa cctccgcaaa aacccgtccc cagtgctaaa
    19621 atcaagccct tactagtccc cttatagacc agatagccct tgacaagaaa aatcaagcag
    19681 aaaattccca gatagttgac aaagagagtg ccccaaggaa agtctggact ggctggtaac
    19741 caggtggaaa ctagatatcg gacaagtccg cccaacatag cagctagaaa aattcctagc
    19801 ggataaaatt gttctttttt catttgatat tttgatcctg ataatcacgc gaacgtttga
    19861 gtatgtccga aaaagtcgct acaatagtct cctgatagcg cttgtcctct acacgactcc
    19921 caaccttttc aaaaataact tcttctctct tagtatctaa aatcggttta cctgaagctt
    19981 tcttataagc aaccaccccc tcaactaaat gcattcgttc ttctaacagt ttgacaattt
    20041 ggtcgtcgat ttgatcgatt tcttgacgaa taatatctaa atccatacag tctcctcctt
    20101 tatttgaatt attgtatcaa aaagccatca attaggctag taaaatccca actctcgata
    20161 agaaaaatgc actgattgat tacatcagcg cacttttatg cttatcctac tttagcaacc
    20221 aattcttctg cgaattgttc caagcgttca atatcttctt cctcggcaga aagatcaact
    20281 ttgacacact cagaaccttt ttctgctcct gttgaaacaa agacacggtc aaagtcatca
    20341 acagccttac agaattcatc gtagaaggta tctcctgagc cgaccactcc gtagattttg
    20401 ccattcaagt tgagatctgc tagatcttcg tagaagtcca tcatctcatc tggcaattca
    20461 ccatcaccat aagtataggt cgcaacgata gcgatgtctg cttccaagaa gtctgaagcg
    20521 tcaacagttg tacattcatc aacatcaaca tccaagccca agtcacgtaa tttgtctgct
    20581 acaatatctg caatttcttc ggtattaccg gtcatactgg caaatacaat ttttgctaat
    20641 gccatatcgt cctcctcaat ttatctttct ccattatatc acatcttttt cattttaaaa
    20701 ataaaaattc ttgtgctaca atgaaatgag aaagaattga ggttatttat ggagatttgc
    20761 caacaaattt tagaaaaaat taaagaatac gacaccatta tcattcatcg tcatatgaaa
    20821 ccagaccctg atgccttggg aagtcaggtg ggattgaaag ccttgctgga acatcatttc
    20881 ccagaaaaaa ccatcaaagc cgtcggtttt gatgaaccaa ctcttacttg gatggctgag
    20941 atggatcttg ttgaagatag agcctaccaa ggcgcacttg tcatcgtctg tgatacagct
    21001 aatactgctc gtatcgatga taagcgctat agtcaaggtg attttctcat taagattgac
    21061 caccatccaa atgatgatgt atacggtgac ctgtcttggg tcgatactag ttcaagtagc
    21121 gctagcgaga tgattaccct atttgcccaa acaacccaac tagccttggc agatcgcgat
    21181 gctgagttgc tctttgcagg aattgtcggt gatacaggtc gcttcctcta cccttctacc
    21241 actgcacgga ctcttcgcct ggctgcttat ttgagagaac ataactttga ctttgcggct
    21301 ctcactcgca aaatggacac tatgagctac aaaattgcta aactgcaagg ctacatctac
    21361 gaccatctgg aagtggatga aaatggtgct gctcgcgtta tcctgagtca agaagtcttg
    21421 aaacaataca atgtaaccga tgctgaaact gcagccattg tcggtgcacc tggacgcatt
    21481 gacagagtga gtctctgggg aatttttgtc gaacaggctg atggccacta ccgagttcgc
    21541 ttacgcagta aaatccatcc tatcaatgaa attgccaagg agcatgatgg tggaggccac
    21601 cctctagcaa gtggtgctaa ttcctatagc ctagaagaaa acgaaatcat ctaccaaaag
    21661 ttaaaaaact tgcttaaaaa ctgataaaat acttgccaaa cttttcagaa tctgatagac
    21721 tagtatggta acaatctatg gctcgcaaag agaccatggc agaaaggaaa tattgcaaaa
    21781 tgaaaaaaga tatccatcca gaatatcgcc cagttgtctt catggacaca actactggtt
    21841 accaattcct tagcggttca acaaaacgct ctaacgaaac agttgagttc gaaggcgaaa
    21901 cttacccatt gatccgtgtg gaaatttcat cagactcaca cccattctac actggacgtc
    21961 aaaagttcac tcaagcagat ggacgcgtgg atcgtttcaa caaaaaatac ggtctcaaat
    22021 aatgataaaa gaacagtttc gactgttctt ttttgtttct tgaaatcaac tgctgttttc
    22081 atgttccaga ctcatctgta ggttcggttt ccatgctact aggcaggaag gaaatagctg
    22141 tttcaacacg tccataatga gctgtactat tgtcacgaac cacactttca ttgatggtcc
    22201 aagtggaatt cattttctta aaagcttctc ggactttttc caaatctttg gaggcaatgg
    22261 cctgctctaa ggtttcaaaa cgaggactta tactcatctg ctttcacaaa gcattctagt
    22321 ccatctccga ttaacgatgg actttatcac ctccttctcc agtccttgta taacatcttg
    22381 gagttgattc atgacatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg
    22441 aatctctttc cacacttgtt caatgggttc atctctggtg tgtatggagg aataaaggta
    22501 aaatcaatat tagtcggaat atttaaggta cttgatttat gccatatagc attgtccata
    22561 acgagtaaaa ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc acactccttt
    22621 ttataagcct gaaataaggc atcaattgta acaaattctc ctgcctctgc agccttcaaa
    22681 tggcgagcaa gaaaggcttt ctcttcctca actgtcatat atgcatgatt acgaccacca
    22741 cgtgtttctt gaaggagaga gtcgagtccg aactcctcat atttttttac atttcgccaa
    22801 atcgttgttt gattacagtc taaaagttct ataatctctt tataagattt gcccatcaga
    22861 cgaaatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca ttgttagaaa
    22921 tcgatttgac tgtcctgatc gatttgctct gttcttgttt cgttttacta tagaacgatt
    22981 tga
//
LOCUS       DAVKWS010000029        22834 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKWS010000029 DAVKWS010000000
VERSION     DAVKWS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.029"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            80..2302
                     /gene="recJ"
                     /locus_tag="VW852_001237"
     CDS             80..2302
                     /gene="recJ"
                     /locus_tag="VW852_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV0272385.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2477..4138
                     /locus_tag="VW852_001238"
     CDS             2477..4138
                     /locus_tag="VW852_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0272386.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4207..4986
                     /locus_tag="VW852_001239"
     CDS             4207..4986
                     /locus_tag="VW852_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV0272387.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5098..6318
                     /gene="murM"
                     /locus_tag="VW852_001240"
     CDS             5098..6318
                     /gene="murM"
                     /locus_tag="VW852_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV0272388.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFRDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6323..7555
                     /gene="murN"
                     /locus_tag="VW852_001241"
     CDS             6323..7555
                     /gene="murN"
                     /locus_tag="VW852_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV0272389.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7634..8335
                     /locus_tag="VW852_001242"
     CDS             7634..8335
                     /locus_tag="VW852_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV0272390.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8375..10222
                     /gene="uvrC"
                     /locus_tag="VW852_001243"
     CDS             8375..10222
                     /gene="uvrC"
                     /locus_tag="VW852_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV0272391.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10212..11045
                     /locus_tag="VW852_001244"
     CDS             10212..11045
                     /locus_tag="VW852_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0272392.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKTQGASEYWLMGDIFLPGP
                     GANDLVALLKELPITASVRGNWDDCVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11227..12027)
                     /locus_tag="VW852_001245"
     CDS             complement(11227..12027)
                     /locus_tag="VW852_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0272393.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINHWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12198..12803
                     /locus_tag="VW852_001246"
     CDS             12198..12803
                     /locus_tag="VW852_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV0272394.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12816..14216
                     /gene="pepV"
                     /locus_tag="VW852_001247"
     CDS             12816..14216
                     /gene="pepV"
                     /locus_tag="VW852_001247"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV0272395.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14270..14848
                     /locus_tag="VW852_001248"
     CDS             14270..14848
                     /locus_tag="VW852_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV0272396.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15008..15160
                     /locus_tag="VW852_001249"
     CDS             15008..15160
                     /locus_tag="VW852_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0272397.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15203..15295
                     /locus_tag="VW852_001250"
     CDS             15203..15295
                     /locus_tag="VW852_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0272398.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15466..16791
                     /gene="brnQ"
                     /locus_tag="VW852_001251"
     CDS             15466..16791
                     /gene="brnQ"
                     /locus_tag="VW852_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV0272399.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16889..17926
                     /locus_tag="VW852_001252"
     CDS             16889..17926
                     /locus_tag="VW852_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV0272400.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEEGIELPVTTHFAFSVFEE
                     VGHGANSNIPSQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVVLA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVMATERM
                     VDAYLKSALVD"
     gene            17928..18389
                     /locus_tag="VW852_001253"
     CDS             17928..18389
                     /locus_tag="VW852_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0272401.1"
                     /translation="MCLICQRIEWIKAGENPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KEHVTELHHMETSVKLRFLEEMSLVQEAVAKTFKAEKMNIELLGNGDAHAHWHLFPRR
                     SGDMRGHGLNGRGPVWWVPWEEMAAEDCQVKSHELEQMIKALSDELEKYTV"
     gene            18415..19131
                     /locus_tag="VW852_001254"
     CDS             18415..19131
                     /locus_tag="VW852_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV0272402.1"
                     /translation="MKKRYIVLSGLLAVTLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(19171..19308)
                     /locus_tag="VW852_001255"
     CDS             complement(19171..19308)
                     /locus_tag="VW852_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272403.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            19440..19865
                     /gene="rplK"
                     /locus_tag="VW852_001256"
     CDS             19440..19865
                     /gene="rplK"
                     /locus_tag="VW852_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV0272404.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            20073..20762
                     /gene="rplA"
                     /locus_tag="VW852_001257"
     CDS             20073..20762
                     /gene="rplA"
                     /locus_tag="VW852_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV0272405.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(20851..22157)
                     /locus_tag="VW852_001258"
                     /pseudo
     CDS             complement(20851..22157)
                     /locus_tag="VW852_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22507..22644
                     /locus_tag="VW852_001259"
                     /pseudo
     CDS             22507..22644
                     /locus_tag="VW852_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         6815 a         4208 c         5292 g         6519 t
ORIGIN      
        1 aaaagcaatc tggatagaga aaacgtctaa atcatgttat aatgaagcaa tagaattctt
       61 agaaagagtg gatgtctttt tgataacacc tacttatgaa tggcagtttg ccctgcaggt
      121 agaagatgcg gattttacaa agatagccaa gaaggctgga ctgggtcctg aggtggctcg
      181 gttattgttt gagagaggga ttcagaacca agaaagtctg aagaagtttt tagaaccttc
      241 cttggaggac ttacatgatg cttatctgct ccatgatatg gacaaggcag tggagcggat
      301 tcgtcaggct attgaagaag gggaaaatat tctcgtttat ggagactatg atgcggatgg
      361 catgacttcg gcttctattg tgaaggaaag tttggaacaa cttggtgctg agtgccgagt
      421 ttacctgcca aatcgtttta ccgatggcta tggccctaat gctagtgttt ataaatactt
      481 tatcgagcaa gaagggattt ccttgattgt gacggtggac aatggggttg ctggtcatga
      541 ggctattgca ttggctcagt ctatgggagt agatgtcatt gtgacagacc atcattccat
      601 gcctgaaacc ctgccagatg cttatgctat tgtccatcct gaacatccag atgcggatta
      661 tccttttaaa tatttggctg gttgtggagt tgctttcaag ttggcttgtg ccctgttaga
      721 agaagtgcaa gtggaattgc ttgatttggt cgctattgga actattgcag atatggtgag
      781 tctgacggat gaaaatcgta tcttagttca atatggtctg gaaatgttgg gtcataccca
      841 gcgcattggt ctgcaagaaa tgctggacat ggctgggatt gctgccaacg aagtaacaga
      901 agaaacggtt ggtttccaga ttgctcctcg tttgaatgcc ttgggccgct tggatgatcc
      961 caatcctgcc attgatttgt tgactggatt tgatgatgag gaagcgcatg agattgccct
     1021 tatgattcac cagaaaaacg aagagcgcaa ggaaatcgtt cagtctatct atgaagaagc
     1081 caagaccatg gtggatcctg agaagaaggt tcaggtcttg gccaaggaag gctggaatcc
     1141 tggggttcta ggaatcgtgg ctggtcgttt attggaagaa ttgggacaga cagtcattgt
     1201 tcttaatata gaagacggtc gtgccaaggg cagtgctcgt agtgtggaag cggtcgatat
     1261 ttttgaagct ctggatcccc atcgagacct cttcatcgcc tttggaggtc atgcaggtgc
     1321 agcgggtatg acgctggaag ttgagcaact ctcagattta tctcaggttt tggaagatta
     1381 tgttcgtgaa aaaggtgcag atgctggtgg caagaataag ttaaacctag atgaagagtt
     1441 ggatttggag gcacttagct tggaaacggt caaaagtttt gaacgtttag ctccttttgg
     1501 aatggataat cagaaaccta ttttttatat caagaatttt caggtcgaaa gtgctcgtac
     1561 tatgggggca ggtaatgccc atctaaagct gaaaatttcc aagggtgagg cgagttttga
     1621 agtggtagcc tttggtcaag gcagatgggc gacagagttt tctcaaacca agaatctaga
     1681 gttagcggtt aaattgtctg tcaaccaatg gaatggccaa actgccctcc agttgatgat
     1741 ggtggatgcg cgagtggaag gtgttcaact ttttaacatt cgtggaaaaa atgcagtctt
     1801 gccagaaggt gttccagtct tggattttcc tggagaactg ccaaatcttg cagctagtga
     1861 agctgttgtc gtaaaaaaca ttccagagga tattactcag ctgaagacca tttttcagga
     1921 acagcatttc tctgctgtct atttcaaaaa tgatattgac aagacctatt atctgacagg
     1981 ttatgggact agagatcagt ttgctaaatt gtacaagact atttaccagt tcccagagtt
     2041 tgatattcgc tacaagctga aagatttggc tgcatatctt aatattcaac aaatcttgct
     2101 ggtcaagatg attcaagtat ttgaagaact aggctttgtg acgataaaag atggtgtgat
     2161 gacagtcaat aaagaggcgc caaagcggga gataggagaa agtcaaattt accaaaatct
     2221 caaacaaacc gttaaagacc aagaaatgat ggcgctgggt acggtgcaag aaatttatga
     2281 ttttttgatg gaaaaagagt agaagttagg aaagagttgg gaaatcaact cttttttgaa
     2341 aacagacctt cattttgaaa atcatcaaaa aaatggtata atggtaggaa aagattcggc
     2401 tgaaagtatc agaactttta gaataagagg gtagagttgc cctataatca agataaacta
     2461 agattttgga ggaaaaatga gtaatatcag tttaacaaca cttggtggtg tgcgtgagaa
     2521 tggaaaaaat atgtacattg ctgaaattgg agagtccatt tttgttttga atgtagggtt
     2581 aaaatatcct gaaaatgaac aattaggggt cgatgtggtg attccaaaca tggattacct
     2641 ttttgaaaat agcgaccgta ttgctggggt tttcttgacc cacgggcatg cggatgctat
     2701 tggtgctcta ccttatctct tggcagaggc taaagttcct gtatttgggt ctgagttgac
     2761 cattgagttg gcaaagctct ttgtcaaagg aaatgatgcc gttaagaaat ttaatgattt
     2821 ccatgtcatt gatgagaata cggagattga ttttggtggg acagtggttt ccttcttccc
     2881 tacgacttac tccgttccag agagtctggg aattgtcttg aagacatcgg aaggaagtat
     2941 cgtttataca ggtgacttca aatttgacca aacggctagt gaatcttatg caactgattt
     3001 tgctcgtttg gcagagattg gtcgtgacgg cgtcctggct ctcctcagtg attcggccaa
     3061 tgcagacagc aatattcagg tggctagtga aagtgaagtt agggatgaaa ttacccaaac
     3121 tattgctgac tgggaaggtc gtatcatcgt tgcagctgtt tccagtaatc tttctcgtat
     3181 tcagcagatt tttgacgctg cggataaaac aggtcgacgt atcgtcttga caggatttga
     3241 tatcgaaaat atcgtccgca cagcgattcg tcttaagaag ttgtctttag ccaacgaaat
     3301 tctcttgatt aagcctaaag atatgtctcg ctttgaagac catgagttga ttattcttga
     3361 gacaggtcgt atgggtgaac ctatcaatgg acttcgtaag atgtcgattg gtcgccatcg
     3421 ttatgtagaa atcaaggatg gggacctggt ctatattgct acggctccgt ctattgctaa
     3481 agaagccttt gttgcgcgtg tggaaaatat gatttatcag gcaggtgggg ttgtcaaatt
     3541 gattacccaa agtttacatg tatcagggca cggaaatgtg cgtgatttgc agctgatgat
     3601 caatcttttg caacctaagt acctcttccc tgtccaaggg gagtatcgtg agttggatgc
     3661 tcacgctaag gctgccatgg cagttgggat gttgccagaa cgcattttca ttcctaaaaa
     3721 ggggacgacc atggcttacg agaatggaga ctttgttcca gctggatcgg tttcagcagg
     3781 agatatcttg attgatggga atgccattgg tgatgttgga aatgttgttc ttcgtgaccg
     3841 taaggtcttg tcagaggatg gaattttcat cgtggctatt acagttaacc gtcgtgagaa
     3901 gaaaattgtg gctagggctc gtgttcacac gcgtggattt gtttatctca agaagagtcg
     3961 cgatattctc cgtgaaagtt cagaattgat taaccaaacg gtagaagatt atcttcaagg
     4021 agatgacttt gactgggcag atcttaaagg gaaggttcga gataatttga ccaagtatct
     4081 ctttgaccaa accaagcgtc gtccagctat tttaccagta gtcatggaag caaaataatc
     4141 gttgaaataa acagagagaa agtcgagttt cggctttttc ttatagaaaa atagaaggag
     4201 aaaatcatgg cagtaatgaa aatcgagtat tactcacaag tattggatat ggagtggggg
     4261 gtgaatgtcc tctaccctga tgccaatcga gtggaagaac cagagtgtga agatattccc
     4321 gtcttgtacc ttttgcacgg gatgtctgga aatcataata gttggcttaa gcggaccaat
     4381 gtagaacgct tgcttcgagg aactaatctc atcgttgtta tgcccaatac cagcaatggt
     4441 tggtacaccg atacccagta tggttttgac tactacacgg ctctagcaga ggaattgcca
     4501 caggttctga aacgcttctt ccctaatatg actagcaagc gtgaaaagac ctttatcgct
     4561 ggtctttcta tgggaggcta cggctgcttc aaactggctc ttacgacaaa tcgtttttct
     4621 catgcagcta gtttttcagg tgccctcagc tttcaaaact tttctcctga aagtcaaaat
     4681 ctgggaagtc cagcctactg gagaggtgtt tttggagaga ttagagactg gacaactagt
     4741 ccctattctc ttgaaagtct ggctaaaaaa tcggataaaa agaccaaact ttgggcgtgg
     4801 tgtggcgaac aggatttctt gtacgaagcc aataatctcg cagtgaaaaa tctcaaaaaa
     4861 ctaggttttg atgtgaccta tagccatagt gctggaactc acgagtggta ctactgggaa
     4921 aaacaattgg aagttttttt aacaacccta ccaattgatt tcaaattaga agagagactg
     4981 acttagtttg aacttcagca tagggggagt agaactaaaa taaaatatgt tttcactaga
     5041 cttttcaaac gaaagtagta gaatagtaat aaaatactgg aggaaagaga ttaggaaatg
     5101 taccgttatc aaattggcat tcccacatta gaatatgatc agtttgtcaa agaacatgaa
     5161 ttagccaatg tattacaaag tagtgcttgg gaggaagtta agtctaattg gcaacatgag
     5221 aagtttggtg tttacaggga agaaaaatta ctggcgacag ctagtatttt gattagaact
     5281 cttccgctag gctataaaat gttttacatc ccaagaggac ctatattgga ttatggggat
     5341 aaagaactct tgaattttgt cattcagtct attaagtcct atgctcgcag taagagagcg
     5401 gtttttgtga cttttgaccc aagtatttgc ctatctcaaa gtttaatcaa tcaggaaaag
     5461 acagaatttc ctgaaaatct ggctattatt gatagtttgc aacaaatggg agtaaggtgg
     5521 tcaggaaaaa cggaggaaat gggagacacc attcaacctc gtattcaggc gaaaatatac
     5581 aaggaaaatt ttgaagaaga taaactttcc aagtcaacaa aacaggctat tcgaacagca
     5641 cgaaacaaag ggcttgagat tcaatatggt ggactggaac tattagattc attttcggag
     5701 ttgatgaaaa aaactgagaa gcgaaaagag attcatttga ggaatgaagc ctattataaa
     5761 aaattgttag ataattttag ggacaaggcc tatatcacct tggccacctt ggatgtttct
     5821 aaacgttcgc aagaattaga agaacagtta gcgaaaaata gagccttgga agagaccttt
     5881 actgagtcga ctcgaatttc aaaagtagaa gcgcagaaga aggaaaaaga acgtttgtta
     5941 gaggaattga ccttcttgca ggaatatata gatgtaggtc aagcgagagt tcctttagcg
     6001 gctactttga gtttggaatt tggtactacc tctgtcaata tatatgctgg tatggatgat
     6061 gattttaaac gttacaatgc accaatttta acatggtatg aaacggctcg ctatgccttt
     6121 gagcgaggta tggtttggca aaatttaggt ggtgttgaaa actctctcaa tggtggactt
     6181 tatcatttta aggaaaaatt taatccaacg attgaagaat acttgggtga atttacaatg
     6241 cccactcatc ctctctatcc tctgttaaga cttgctcttg atttccgtaa aacattaaga
     6301 aaaaaacata gaaagtaagt atatggcact aacaacactc acgaaagaag agtttcagac
     6361 ttattctgat caggtttctt ctcgttcctt tatgcaatct gtccagatgg gggatttgct
     6421 agaaaaaaga ggggctcgaa ttgtttatct tgctttgaaa caagaaggag aaattcaagt
     6481 tgcagctctg gtttatagct tgcccatgct gggtggtctg catatggaac tcaattcggg
     6541 gccgatttat acccaacaag atgctcttcc agttttttat gcagagttaa aagaatatgc
     6601 caagcaaaat ggtgtattag agttgcttgt aaaaccttat gaaacttatc aaacttttga
     6661 tagccaaggt aattcaatag atgctgagaa aaaaagtatt attcaaggtt tgactgattt
     6721 aggttatcaa tttgatggct taacaacagg ttacccaggt ggagaaccag attggttata
     6781 ctataaagat ttaactgaat taactgaaaa gagtttgctt aaaagtttta gcaaaaaggg
     6841 taaacccttg gtgaaaaagg ctgaaacctt tggcattcgg ttgaaaaagt taaaacgtga
     6901 agaactatcg atttttaaga atataacaaa agaaacctct gaacgtagag aatatagtga
     6961 taaaagttta gaatattatg agcattttta tgatactttt ggagaacaag cggagtttct
     7021 catagcaagc ttaaattttt cggactatat gagcaaattg caaggtgaac aaagtaaact
     7081 agaagaaaac ttggacaagt tgcgacttga tttgagtaaa aatcctcatt ctgagaaaaa
     7141 acaaaatcaa ctgagagaat attctagtca atttgaaacg tttgaagttc gaaaagcaga
     7201 agcgcgagac ttgattgaaa aatatggaga agaagatatt gttttagctg ggagtttatt
     7261 tgtttatatg cctcaggaaa cgacttatct ctttagtggt tcctacactg agtttaataa
     7321 gttctatgcc cctgccctgc ttcaaaaata tgttatgttg gaaagcataa aacgtggaat
     7381 acctaaatac aacttcctag gcattcaagg gatttttgat ggaagtgatg gtgttttgcg
     7441 ttttaaacag aattttaatg gctatattgt acgcaaagcg ggtactttcc gttaccatcc
     7501 atcgccttta aaatacaaag ctatccagtt actcaaaaaa atagtaggac gttaagatga
     7561 aaaagtcagt atttagattt cttttagctt cttttagtaa aataattctt atttgctaga
     7621 aaggtggaga gacatgcgct ggctttttcg tttgataggg gctttctttt cttttgtgtg
     7681 gcgtttgttt tggcgtctgg tttggatagt tgtgctctta tgtgtgcttg ctttcggact
     7741 tctctggtat ctgaacgggg attttcaagg agcgctaaag caagcagaac ggtcagtaaa
     7801 aattggtcaa caaagtattg accaatggga gaaaacaggg caactgccta agttaagcca
     7861 gacagatagt caccagcatt ctgaaggaag gtgggcacag gcctctgctc gtatttacct
     7921 ggatccgcag atggattcac gctttcaaga ggcttattta gaagcgatcc agaactggaa
     7981 tcaaactggt gcttttaact ttgaactcgt gactgagtct agtaaggcgg atattacggc
     8041 tacggagatg aacgacggag gcactcctgt ggcaggagag gcggaaagtc aaactaatct
     8101 cttaacaggg caattcttgt ccgtaacggt gcggttgaat cattattatt tgtccaatcc
     8161 atactatggc tactcctatg aacgccttgt ccatacggca gaacatgagt taggtcatgc
     8221 gattggcttg gaccatacag atgagaagtc tgtcatgcaa ccagcaggtt ccttttatgg
     8281 tatccaggaa gaggatgttg caaacctccg aaaaatatat gagactagtg agtagggtac
     8341 tatctttccc tacttttttt gctataatgg aactatgaac aacttgatta aatcaaaact
     8401 agagctcttg ccgaccagcc ctggttgcta cattcataag gataaaaatg gcaccattat
     8461 ctatgtagga aaggctaaaa atctgcgtaa tcgagtacgg tcctattttc gtggaagtca
     8521 tgataccaag acagaggctc tggtgtctga aattgtggat tttgaattta tcgtcactga
     8581 gtccaatatt gaggcgcttc tgctggaaat caacctgatc aaggaaaata agcccaagta
     8641 caatatcatg ctcaaggatg ataagtctta tccttttatc aaaatcacca atgagcgcta
     8701 tccacggctt atcattactc gtcaggtcaa aaaggacggt ggtctttatt ttggccctta
     8761 tccagatgtg ggggcagcca atgaaatcaa gcgacttcta gataggattt tcccttttcg
     8821 aaaatgtacc aacccgccct ctaaggtctg tttttattac catatcggcc agtgtatggc
     8881 ccacaccatc tgtaagaagg atgaggctta tttcaagtct atggcccagg aggtttctga
     8941 ttttctgaaa gggcaggata acaaaatcat tgatgagcta aagggcaaaa tggcagcggc
     9001 agcccagact atggagtttg aacgtgcggc ggaataccgt gacctgattc aggctattgg
     9061 aacgcttcga accaagcaac gggtcatggc taaggatctc caaaatcgcg atgtctttgg
     9121 ctactatgtg gataagggct ggatgtgtgt gcaggttttc tttgttcgtc agggcaagct
     9181 catcgagcgc gatgtcaatc tcttccccta cttcaatgat ccagatgagg atttcttgac
     9241 ctatgtggga caattctatc aagaaaaatc tcacctagtt cccaatgagg tactgattcc
     9301 gcaggatatt gacgaagagg ctgtcaaggc cttggtggat tccaagattc ttaagcctca
     9361 acgtggagag aaaaagcagc tggtcaattt agctataaaa aatgcccgtg ttagcctaga
     9421 gcagaagttc aacctgctag agaaatctgt cgagaagacc caaggagcta ttgaaaatct
     9481 aggacgcttg cttcaaatcc caaccccagt tcgcatcgag tccttcgata actccaacat
     9541 catgggaact agtcctgttt cagccatggt ggtctttgtc aacggcaagc ctagtaagaa
     9601 ggattaccgt aagtataaaa tcaagaccgt agtcgggcca gacgactatg ccagtatgcg
     9661 agaggtcatt cgcagacgct atggtcgagt acagcgtgag gctttgactc ctccagattt
     9721 gattgtgatt gatggggggc aaggtcaagt caatattgct aagcaagtca tccaagaaga
     9781 actgggcttg gatattccaa ttgcaggtct gcaaaagaat gataagcacc aaacccatga
     9841 attgctcttt ggagatccgc tggaagtggt ggacttgtct cgcaattctc aggaattttt
     9901 cctcctccaa cgcatccaag atgaggtgca ccgctttgcc atcaccttcc accgccaact
     9961 gcgctccaaa aattctttct catctcaatt ggatgggatt gatggtctgg gacctaaacg
    10021 caagcaaaat ctcatgaggc acttcaagtc tctgaccaaa atcaaggaag ccagtgtgga
    10081 tgagattgtc gaagttggag taccaagagc agtagcagag gctgtgcaaa ccaagttgaa
    10141 cccgcaggaa acagaaatct tgcttcaagt ggcggaagaa agagtagatt accaaacgga
    10201 aggaaaccac aataaaccat aaaatcgcaa ttttatcaga tgttcatggc aatgcgacgg
    10261 cgctagaagc agtgattgca gatgctaaaa ctcaaggagc cagtgaatat tggctcatgg
    10321 gagacatttt cctccctggt ccaggtgcaa atgacttggt cgctctgtta aaggaacttc
    10381 ctatcacggc aagtgttcga ggcaattggg atgattgtgt ccttgaggct ttagatgggc
    10441 aatatggctt agaagaccca caggaagttc agctcttgcg tatgacacag tatttgatgg
    10501 agcgaatgga tcctgcaacg attgtctggc tacgaagctt gcctttgctg gaaaagaaag
    10561 aaattgacgg attgcgcttt tctatctctc ataatttacc tgacaaaaac tatggtggtg
    10621 acttgctagt tgagaatgat acagagaaat ttgaccaact gctagatgcg gaaacggacg
    10681 tggcagttta tggtcatgtt cacaagcagt tgcttcgtta tggaagtcaa gggcaacaaa
    10741 tcatcaatcc agggtcgatt ggcatgccct attttaattg ggaggcgtta aaaaatcacc
    10801 gtgcccagta tgccgtgata gaagttgaag atggggaatt actcaatatc caatttcgta
    10861 aagttgctta tgattacgaa gctgagttag aattggccaa gtccaagggg cttcccttta
    10921 tcgaaatgta tgaagaactg cgtcgtgacg ataactatca ggggcacaat ctggaactat
    10981 tagccagctt aatagaaaag catgggtatg tagaggatgt gaagaatttt tttgattttt
    11041 tgtaagagtt tcctaaaata gccaatgcaa actaaaaaag cgattggctg atcccatcgc
    11101 ttttagtata tcttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgtaggt
    11161 tgctcaaagc acagctttga ggttgcagat aaagctgacg tggtttgaag agattttcga
    11221 agagtattat tgtaactgag attgatctgg gaggtaagaa ccacctagat aggtattgct
    11281 gagtttttcg agggttccgt cttgatagag ttctttgagc gctttatcaa atttctcttt
    11341 aaactctttt tggtcgcttg agaaaatgat ataattgctg gggctatctg cagaaggtaa
    11401 atcaacgact gagaggtcta aaccacggtc cttgataatc ttttgaacgg ataccttgtc
    11461 aaaaactagg aaatcaaact ctccgttagc aaggtctagg attcgtttac caatatcctc
    11521 accagaaaaa tcaattgtag cgggattatc agtgtgtttc tgattccagt gattgatgaa
    11581 ttgagcgtta gaagttccgg tatcctcttg tgttgtttta ccagcgatct ggtcaagaga
    11641 agtcaaagga tttttcttgt tgctgacaag gacgagggga ttgttggaaa ttggaagcga
    11701 gtaaaggtat ttttcagcac gctcttttgt gtaactcaag ttattggccg cagcctgata
    11761 gtgaccagaa tcaagtcctg ggaagatgct ctcccaagca gttctttgga attgaatctc
    11821 gtagtcgctg agtttttcat ctactgcctt taaaacttcg atatcgaagc ctgtcagatt
    11881 gcccttgtct tcgtagtcaa atggtggcac gtcgccagct gtagcaagga cgattgtctt
    11941 ttgagagctg gactctttgg gtgtagcttg attcccacag gcaaccaaaa atggtaggat
    12001 agctagtaat aggctaaatt ttttcatact gtctccattc aaatgtaaag tacttgctag
    12061 tttatcagaa actttcctga taaaccaata tatatctttt atgtctgtga tagaaaaact
    12121 taatcatgaa aaatccctgc aagctctttc aaattatggt agaatggaag cagtagaatt
    12181 agaaaaggaa atcgattatg aaatttcttg aattaaataa aaaacgtcat gcgaccaagc
    12241 atttcactga taagcctgtt gatccaaaag atgtgcgtac ggctatcgaa attgcaacct
    12301 tggcgccaag cgcccacaac agccagcctt ggaaatttgt agttgttcgt gagaaaaatg
    12361 ctgaactggc aaagttggct tatggttcaa actttgagca ggtatcatca gcacctgtaa
    12421 ccattgccct atttacagat acagaccttg ccaaacgcgc tcgtaagatt gcccgagttg
    12481 gtggtgctaa caatttctca gaagaacaac ttcaatactt catgaaaaat ttgccagctg
    12541 aatttgcacg ctacaatgaa caacaagtca gcgactacct agcactcaat gcaggtttgg
    12601 ttgctatgaa cttggttctc gctcttacag accaaggaat tggttccaat attatccttg
    12661 gttttgacaa atcaaaagtt aatgaagttt tggaaatcga agaccgtttc cgcccagaac
    12721 tcttgatcac agtgggttac acagacgaca aattggaacc aagctaccgc ttgccagtag
    12781 atgaaatcat tgaaaaaaga taggaagaag aaaaaatgat agcaattgat tttacagcag
    12841 aagtagaaaa acgcaaagaa gacctcttgg ctgacttgtt tagccttttg gaaatcaact
    12901 cagaacgtga tgatagcaag gctgatgccc aacatccatt tggacctggt ccagtaaaag
    12961 ccttggaaaa attccttgaa atcgcagacc gtgatggcta cccaactaag aatgtcgata
    13021 actacgcagg acattttgag tttggtgatg gagaagaagt tctcggaatc tttgcccata
    13081 tggacgtggt tccagctggt agcggttggg atacagatcc ttacacacca actatcaaag
    13141 atggtcgcct ttatgcgcgc ggggcttcgg acgataaggg tcctacaaca gcttgttact
    13201 atggtttgaa aatcatcaaa gaattgggtc ttccaacttc taagaaagtt cgcttcatcg
    13261 ttggaacaga cgaagaatca ggctgggcag acatggacta ctactttgag cacgtaggac
    13321 ttgcgaagcc agactttggt ttctctccag atgctgaatt tccaatcatc aatggtgaaa
    13381 aaggaaatat cacggaatac ctccactttg caggagaaaa tacaggtgct gcccgtcttc
    13441 acagctttac aggtggttta cgtgaaaata tggtaccaga atcagcaaca gcagtcgttt
    13501 caggtgattt ggctgacttg caagctaaac tagatgcctt tgttgcagaa cacaaactta
    13561 gaggagaact ccaagaagaa gctggcaaat acaaggtgac gatcattggt aaatcagccc
    13621 acggtgctat gcctgcttca ggtgtcaatg gcgcaactta ccttgccctc ttcctcagcc
    13681 agtttggctt tgctggtcca gccaaagact accttgacat cgcaggtaaa attctcttga
    13741 acgatcatga gggtgaaaat cttaagattg ctcatgtgga tgaaaagatg ggtgctcttt
    13801 ctatgaatgc cggcgtcttc cgcttcgatg aaacaagtgc tgataatacc attgccctta
    13861 acatccgcta tccaaaagga acaagtccag aacaaatcaa gtcaatccta gaaaacttgc
    13921 cagttgcttc tgtcagtcta tcagaacatg gtcatacccc tcactatgtg ccaatggaag
    13981 atccacttgt gcaaaccttg ttgaatgtct atgaaaaaca aacaggcctt aaaggtcatg
    14041 aacaagtcat cggtggtgga acctttggtc gcttgttaga gcgcggagtt gcctatggtg
    14101 ctatgttccc agactcaatt gataccatgc accaagccaa tgaatttatt gccttggacg
    14161 atctcttccg agcagcagca atttatgccg aagctattta cgaattgatc aaataaaacg
    14221 atagaagtct gagatcttat gcttggactt ctttttggag ggaaagtaga tgtctcaaat
    14281 cgaaagaatc aaacaggcta tcatggcgga ttcgcagaat gccagctata cagagcgtgg
    14341 cattgagcct ctctttgcag cgccaaaaac tgctcgcatc aatatcatcg gtcaggctcc
    14401 gggacttaaa actcaagaag caggccttta ctggaaagat aaaagtggtg accgtttgcg
    14461 ggactggcta ggtgtggatg aagatacctt ttacaattca ggttattttg ctgttttgcc
    14521 tatggatttc tactttccag gacatggcaa gtcgggtgat cttccgcctc gtacaggttt
    14581 tgcagaaaaa tggcatccgc agctcttaca ggaattgcct gatattcagt taaccctctt
    14641 gattgggcaa tatgcccaag cctactattt acaggagaaa atcagtggga aggtaacgga
    14701 gagggtgaaa cactataaag actatctgcc agcctatttt ccgctagttc acccatcacc
    14761 acgaaatcaa atctggatgg ccaaaaatcc ttggtttgag gcagaagtag tgccagattt
    14821 gaaaaaaaga attaaaacca ttttatagtc aatgaaaatc aaagagcaaa ctaggaagct
    14881 agtcgtaggc tgctcaaagt acagctttga agttgcagat aaaactgacg aagtcggtaa
    14941 catacgcacg gtaaggcgac gctgacgtgg tttgaagaga ttttcgaaga gtattagaag
    15001 aaaaagaatg aaagaaatag cctttgacgc attttaccag ctttaccaaa acgaccagct
    15061 ttctctagtg gatgtgagag aagtggatga gtttgcagct cttcatttag aaggtgccca
    15121 caacctaccg cttagtcaat tggctgatag ttatgattaa ttggacaaag atcgcttgca
    15181 ttatattatt tgcaaatctg gaatgagatc ggcgcgtgct tgccaattcc tattagaaca
    15241 aggttataat gttatcaatg tccagggtgg catgttagcc tttgaagaac tttaaaattt
    15301 tgcatttctc ctacttggtg tggactgggt aggagagttt tatttttaga taattcttat
    15361 ttttaagaaa attgaaaaca tttaatattt gcctcgtgat gcttttttca gactcctaat
    15421 cgtggtatac taggtcagta ttttataaat atgaaggaga tttttatggc taaaaaaggt
    15481 accctaacag gtttgctcct gtttggaata ttttttggtg cggggaactt gatttttccg
    15541 ccttctctag gtgctctatc tggagaacat tttcttcctg ccatcgcagg ttttgtcttt
    15601 tcaggcgttg gtatcgccgt cttgaccctt attattggaa cgctaaatcc taaaggatat
    15661 atctacgaga tttcaacgaa gatagcgcct tggtttgcga ctctttacct ctcagttctt
    15721 tacttgtcaa tcggtccatt ctttgctacc ccacgtactg ctacaacagc ttacgaagta
    15781 gggattagcc cccttttgtc ggatgcaaat aaaggacttg gcttgattgt atttacggtt
    15841 ctgtattttg cggcagccta tttgatttcg cttaatccat caaaaatctt agaccgcatt
    15901 ggacgtattt taacgccagt ctttgcaatt ttgattgtta tcttggtcgt tctgggagct
    15961 atcaaatatg gtggaacaag tcctcaagct gcttcagctg cttatcaagc ttctgccttt
    16021 ggtacaggtt tcctagaagg ttacaatact ttggacgccc ttgcctcagt ggcctttagc
    16081 gtaatcgcag ttcaaacctt gaaacaactt ggattttcaa gtaagaaaga atacatttca
    16141 actatttggg ttgttggtat cgttgttgcc cttgccttca gcgctcttta catcggttta
    16201 ggttttcttg gaaatcattt cccagtacca gctgaagcga tgaagggtgg aacaccaggt
    16261 gtttacatct tgtcacaagc cactcaagaa atctttggct caacagctca actcttcctt
    16321 gcagctatgg ttaccgtaac ctgcttcaca acgactgttg gtttgattgt gtcaacagct
    16381 gaattcttta atgagcgctt cccacaaatc agctacaagg tttatgcgac agcctttacc
    16441 ttgattggat ttgctattgc caatttgggt cttgatgcga ttatcaagta ctcaattcca
    16501 gtactggtta tcttgtaccc aatcacgatt gctatcgtta tgattgtcat tgtcaacaaa
    16561 tttgttgccc tttcaaaacc aggtatgcag ttgacaattg ctgtggttac agttattgcc
    16621 attgcaagcg tactaggaag ctcgtttaag gttgagtttc ttgcaaatct tgttagcgtt
    16681 cttccttttg ccaaggcatc tctcccatgg ttggtgccag ccattgttgg aatcttgctc
    16741 tcattggttc taccaaacaa gcaagaaagc gatgtttttg aaatggaata atcacttaaa
    16801 tcacttttgt agccaagtct acaggagtga ttttcttttt ttatccgatg ataaatgtgt
    16861 tataataggt agcgaaagag gtgaagaaat gaatcaaaca gtagaatata tcaaagaact
    16921 gacagccatc gcatcgccaa cgggcttcac tcgtgaaatt tcgaattatt tagtcaagac
    16981 tctagaaggt tttggttacc agccagttcg cacagctaag ggtggtgtca atgtgaccat
    17041 caaaggtcaa aatgatgagc aacatcgcta tgtgactgcc catgtggata cgctgggtgc
    17101 tattgttcgt gctgtcaaac cagatggtcg tcttaaattg gaccgtatcg gtggttttcc
    17161 ttggaacatg attgaaggtg aaaactgtac cgttcatgtg gctagcacag gtcaaaaggt
    17221 atcaggaacc atcctcatcc accaaacttc ttgccatgtc tacaaggatg caggaactgc
    17281 agaacgcacg caggacaata tggaagtgcg tttggatgta aaagtaagca atgaaaaaga
    17341 aactcgtgct cttgggattg aggtcggtga ttttatcagc ttcgatccac gaactgtcgt
    17401 gacagagact ggttttatca agtctcgcca cttggatgac aaggtcagcg cagcaatttt
    17461 gctcaatctt cttcgtgttt ataaggaaga ggggattgaa ttgccagtaa caactcattt
    17521 tgctttttca gtcttcgaag aggtgggcca cggtgcaaac tccaatattc caagtcaggt
    17581 agtagaatat ctggctgtag atatgggagc tatgggagat gaccagcaga cggatgagta
    17641 tacagtgtct atctgtgtca aggacgcttc aggtccctat cactatgact tccgtcaaca
    17701 cttggtagtt ttggcgaagg agcaagatat tccatttaag ctcgacattt atccatttta
    17761 cggttcggat gcttcggcag ctatgtctgc aggagcagaa gtaaagcatg ctctccttgg
    17821 agctggtatt gagtcaagtc actcttacga acgaacacac attgattcag taatggcgac
    17881 tgagcgtatg gtcgatgctt atcttaagag tgcattggta gactaatatg tgcttgattt
    17941 gtcaaagaat tgaatggatc aaggcagggg aaaatcccta ctttgtaaaa gaactagaaa
    18001 caggctatgt tgttattgga gaccaccaat actttaaggg ctatacctta tttctagcaa
    18061 aagagcatgt cacagaactc catcatatgg agacttctgt aaaacttcgt tttctagagg
    18121 aaatgagttt ggtccaagaa gctgttgcca aaacgtttaa agctgaaaag atgaacattg
    18181 aacttctagg aaatggagat gcccacgctc actggcatct ctttccaaga cggtcaggtg
    18241 atatgagggg tcatggattg aatggccgtg gtccagtctg gtgggtgccc tgggaagaaa
    18301 tggcggcaga agattgccaa gtgaaatccc atgagttgga acaaatgatt aaagccttat
    18361 ccgatgaatt agagaagtac acggtctaag agaggaagaa tagaggaaga aaaaatgaaa
    18421 aaaagataca tcgttttatc tggtttgctg gcagtaaccc tagcagcttg ttcacaagaa
    18481 aaagcaaaaa atgaagatgg cacagctaag acagaacaga cagccaaagc tgatgggaca
    18541 gtcggcagta agtctcaagg ggctgcccag aaaaaagcag aagtggtcaa taaaggtgat
    18601 tactacagca tccaaggaaa atacgacgaa atcatcgtag ccaataaaca ttatcctttg
    18661 tcgaaagact ataatcccgg ggaaaatcca acagccaagg ctgagttggt caaactcatc
    18721 aaagcgatgc aagaggcagg tttccctatt agtgatcatt acagtggttt tagaagttat
    18781 gaaactcaga ccaagctcta tcaagattat gtcaaccaag atggaaaggc agcagctgac
    18841 cgttactctg cccgtcctgg ctatagcgaa caccagacag gcttggcctt tgatgtgatt
    18901 gggactgatg gtgatttggt gacagaagaa aaagcagccc aatggctctt ggatcatgca
    18961 gctgattatg gctttgttgt ccgttatctc aaaggcaagg aaaaggaaac aggctatatg
    19021 gctgaggaat ggcacctgcg ttatgtagga aaagaagcta aagaaattgc tgcaagtggt
    19081 ctcagtttgg aagaatacta tggctttgaa ggcggagact acgtcgatta atactcttcg
    19141 aaaatctctt caaaccacgt cagcgtcgtc ttacctactg actgcgtcgg ttctattcac
    19201 aacctcaaaa cagtgttttg agctgacttc gtcagtttta tctgcaacct caaagctgta
    19261 ctttgagcag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaaaa
    19321 ataaactttt ctcttgcaat tccagataaa tagtgtataa tgaatgggta tgcgaaaaac
    19381 atacttgtgg gaggtaaaaa tctctaatta ccgccaaaac cacaaaggag gatttaaaaa
    19441 tggctaaaaa agtcgaaaaa cttgtaaaat tgcaaatccc tgctggtaaa gctacaccag
    19501 ctccaccggt tggacctgct cttggtcaag ctggtatcaa catcatggga ttcacaaaag
    19561 agttcaacgc tcgtacagct gaccaagctg gtatgatcat tccagttgtt atctcagttt
    19621 acgaagataa atcatttact ttcgttacga aaacaccacc agctgctgtt cttttgaaaa
    19681 aagctgcagg tgttgaaaaa ggatcaggta cacctaataa aactaaagtt gctacagtta
    19741 ctcgtgcaca agtacaagaa attgcagaaa ctaagatgcc agatttgaac gcagcaaacg
    19801 tagagtctgc aatgcgtatg atcgaaggta ctgctcgttc tatgggattc actgttgttg
    19861 actaatcaat aacacagtag aaaatctcac agcagtctat tagttgcttt tcatactagg
    19921 caagtgactg aggcttgtac ttgggtacag caagggggct taaggccgta ggaagagaaa
    19981 aatagtagac tgaaaacccg caagacttca tcatttcgag aagtgacgtg ggagatgaaa
    20041 atcgattgaa ccacttacaa ggagaataga aaatggctaa aaaaagcaaa caacttcgtg
    20101 ctgctcttga gaaaatcgac agcacaaaag catacagcgt agaagaagct gtagcacttg
    20161 caaaagaaac taactttgca aaatttgacg caactgtaga agttgcttac aacttgaaca
    20221 tcgacgttaa aaaagctgac caacaaatcc gtggagcaat ggtattgcca aacggtactg
    20281 gtaaaacttc acgtgttctt gttttcgcac gtggtgcaaa agctgaagaa gcaaaagctg
    20341 ctggtgcaga ctttgttggt gaagatgacc ttgttgctaa aatcaacgac ggttggttgg
    20401 acttcgacgt agttatcgct acacctgata tgatggctct tgttggacgt cttggacgtg
    20461 tccttggacc acgtaacttg atgccaaacc ctaaaactgg tactgtaaca atggatgttg
    20521 ctaaagcagt tgaagagtct aaaggtggta aaatcactta ccgtgctgac cgtgcaggta
    20581 acgttcaagc aatcatcggt aaagtatcat ttgaagctga aaaattggtt gaaaacttta
    20641 aagctttcaa cgaaacaatc caaaaagcaa aaccagctac agctaaagga acttacgtaa
    20701 caaacttgac tatcacaact actcaaggtg ttggtatcaa agttgatgta aactcacttt
    20761 aatcagtagt taaagtaatg taaaaaagtt gaagacgcta tgtctcaact ttttttggct
    20821 ctttgtcaac tgtagtgggt tgaaaaaaag ctaagctcga gaaaggacaa attttgtcct
    20881 ttcttttttg atattcagag cgataaaaat ccgttttttg aagttttcaa agtttcgaaa
    20941 accaaaggca ttgcgcttga taagtttgat gagattattg gtcgcttcca gtttggcatt
    21001 agaatagtgt agttgaaggg cgttgacaat cttttcttta tctttgagga aggttttaaa
    21061 gacagtctga aaaataggat gagcctgctt aagattgtcc tcaataagtc cgaaaaattt
    21121 ctctggttcc ttattctgga agtgaaaaag caagagctga tagagctgat agtggtgttt
    21181 caagtcttcc gaatagctca aaagcttgtt taaaatctct ttattggtta agtgcatacg
    21241 aaaagtagga cgataaaatc gcttatcact cagtttacgg ctatcctgtt gaatgagttt
    21301 ccagtagcgc ttaatagcct tgtattcatg ggattttcga tgaaactgat tcatgatttg
    21361 gacacgcaca cgactcatag cacggctaag atgttgtaca atgtgaaagc gttcaagaac
    21421 gattttagca ttcgggagtg aaacagtctg ggagactgtt tcagcctgag cctagaaatt
    21481 tgaaagcgaa gctgtttagc caagtcatag taaaggctaa acatatccat agtaatgatt
    21541 ttgacgcgac atcggacagc tctatcgtag cgaagaaagt gatttcggat gacagcttgt
    21601 gttctgcctt taagaatagt gataatatta agattatcaa aatcttgcgc aatgaagctc
    21661 atctttccct ttgtaaaagc atactcatcc caagacataa tctcaggaag acaagaaaaa
    21721 tcatgtttaa agtgaaaatc attgagctta cgaataacag ttgaagttga gatggaaagc
    21781 tgatgggcaa tatcagtcat agaaatcttt tcaattaact tttgagcaat cttttggttg
    21841 atgatacgag ggatttggtg atttttcttt accaggggag tctcagcaac catcattttt
    21901 gaacagtgat agcacttgaa acggcgtttt ctaaggagaa ttctagtagg cataccagtc
    21961 gtttcaagat aaggaatttt agaaggtctt tgaaagtcat atttcttcaa ttggtttccg
    22021 cactcagggc aagatggggc gtcgtagtcc agtttggcga tgatttcctt gtgtgtatcc
    22081 ttattgatga tgtctaaaat ctggatatta gggtctttaa tgtctagtaa ttttgtgata
    22141 aaatgtaatt gttccatatg aatctttcta atgagttgtt ttgtcgcttt tcattatagg
    22201 tcatatggga ctttttttct acacaaaata ggctccataa tatctatagg ggggttaccc
    22261 actacaaata ttatagagcc ctttttttga tgtacgacgg gcatgtcgta tatctgaggt
    22321 gtaagtcctc ggtgggcacc tactaccggt gaacccaata gcgattccca agtctgacta
    22381 tcgtgaggta gcggattaaa acggtctggg gatagaccgt tttaagtctg acgctggaaa
    22441 taagaatcgt cagaggaagg gatagcgaaa tcgtggctct acgaacagga acgtgataat
    22501 aaggcctata tagcggataa ggtatctggc tggggcgacc actatcaatt agtatctcag
    22561 aagtcggtac ttaaacgtgc tatatcaaaa ccagtccttg aaaaacgtgg actggtttcg
    22621 tgtttggatt attaccttga atgacatgcg ttaacagcta gttgaaccgc cgtatgccga
    22681 acggcacgta cggtggtgtg agaggggcta gagattatcc cctactcgat ttcgaaatct
    22741 agtggaatga atctggaata gtccatcgag ctttctaata ctcttcgaaa atctcttcaa
    22801 accacgtcaa cgtcgccttg ccgtgcgtat ggtt
//
LOCUS       DAVKWS010000030        21681 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKWS010000030 DAVKWS010000000
VERSION     DAVKWS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.030"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            192..356
                     /locus_tag="VW852_001260"
     CDS             192..356
                     /locus_tag="VW852_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0272406.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            440..1006
                     /gene="amaP"
                     /locus_tag="VW852_001261"
     CDS             440..1006
                     /gene="amaP"
                     /locus_tag="VW852_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV0272407.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            1018..1188
                     /locus_tag="VW852_001262"
     CDS             1018..1188
                     /locus_tag="VW852_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV0272408.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1227..1835
                     /locus_tag="VW852_001263"
     CDS             1227..1835
                     /locus_tag="VW852_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0272409.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1866..2069
                     /locus_tag="VW852_001264"
     CDS             1866..2069
                     /locus_tag="VW852_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0272410.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2179..2451)
                     /locus_tag="VW852_001265"
                     /pseudo
     CDS             complement(2179..2451)
                     /locus_tag="VW852_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2655..2942
                     /locus_tag="VW852_001266"
     CDS             2655..2942
                     /locus_tag="VW852_001266"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV0272411.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3220..3990)
                     /locus_tag="VW852_001267"
     CDS             complement(3220..3990)
                     /locus_tag="VW852_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV0272412.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(4005..4631)
                     /locus_tag="VW852_001268"
                     /pseudo
     CDS             complement(4005..4631)
                     /locus_tag="VW852_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(4537..5139)
                     /locus_tag="VW852_001269"
     CDS             complement(4537..5139)
                     /locus_tag="VW852_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0272413.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            complement(5149..5466)
                     /locus_tag="VW852_001270"
     CDS             complement(5149..5466)
                     /locus_tag="VW852_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0272414.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5483..5800)
                     /locus_tag="VW852_001271"
     CDS             complement(5483..5800)
                     /locus_tag="VW852_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0272415.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(5815..6711)
                     /locus_tag="VW852_001272"
     CDS             complement(5815..6711)
                     /locus_tag="VW852_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV0272416.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(6714..7976)
                     /locus_tag="VW852_001273"
     CDS             complement(6714..7976)
                     /locus_tag="VW852_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0272417.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            8256..9434
                     /locus_tag="VW852_001274"
     CDS             8256..9434
                     /locus_tag="VW852_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0272418.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(9662..10108)
                     /locus_tag="VW852_001275"
     CDS             complement(9662..10108)
                     /locus_tag="VW852_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272419.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            10178..10837
                     /locus_tag="VW852_001276"
     CDS             10178..10837
                     /locus_tag="VW852_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV0272420.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSKVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            complement(10889..12040)
                     /locus_tag="VW852_001277"
     CDS             complement(10889..12040)
                     /locus_tag="VW852_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV0272421.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(12055..12786)
                     /locus_tag="VW852_001278"
     CDS             complement(12055..12786)
                     /locus_tag="VW852_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272422.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            13015..13116
                     /locus_tag="VW852_001279"
                     /pseudo
     CDS             13015..13116
                     /locus_tag="VW852_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(13088..13864)
                     /locus_tag="VW852_001280"
     CDS             complement(13088..13864)
                     /locus_tag="VW852_001280"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV0272423.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(13857..15080)
                     /gene="trpB"
                     /locus_tag="VW852_001281"
     CDS             complement(13857..15080)
                     /gene="trpB"
                     /locus_tag="VW852_001281"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV0272424.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(15058..15657)
                     /locus_tag="VW852_001282"
     CDS             complement(15058..15657)
                     /locus_tag="VW852_001282"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV0272425.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAV
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(15644..16411)
                     /gene="trpC"
                     /locus_tag="VW852_001283"
     CDS             complement(15644..16411)
                     /gene="trpC"
                     /locus_tag="VW852_001283"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV0272426.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(16408..17412)
                     /gene="trpD"
                     /locus_tag="VW852_001284"
     CDS             complement(16408..17412)
                     /gene="trpD"
                     /locus_tag="VW852_001284"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV0272427.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIARVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(17423..17989)
                     /locus_tag="VW852_001285"
     CDS             complement(17423..17989)
                     /locus_tag="VW852_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV0272428.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDDRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(17986..19347)
                     /gene="trpE"
                     /locus_tag="VW852_001286"
     CDS             complement(17986..19347)
                     /gene="trpE"
                     /locus_tag="VW852_001286"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV0272429.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTIILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(19986..20066)
                     /locus_tag="VW852_001287"
                     /pseudo
     CDS             complement(19986..20066)
                     /locus_tag="VW852_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20668..21669)
                     /locus_tag="VW852_001288"
     CDS             complement(20668..21669)
                     /locus_tag="VW852_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0272430.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDREKGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         6710 a         4636 c         3613 g         6722 t
ORIGIN      
        1 tttttaaaat tatataataa taacaatcga aataattgac ttttctatat taaagttata
       61 taatagtatt aatcaaagaa attgattttt tgatattaaa ataaaaaagg agggtaggca
      121 gtgttgtgat caattattgc tggaggtctt attggtctct tggcaggtaa aatcactaaa
      181 aaagtagttc tatgggaatc atcgcaaatg tattcgctgg tttagtcggg gcatatgcag
      241 gacaatctct tttaggtagt tggggtccat caatcgctgg aatggctttg ctcccatcta
      301 ttgtaggtgc agcgattgtg attactgtag tgtcattctt tacaggtaga aagtaaactt
      361 ttcgccagta aagttagcaa actattttta aatcaatgac gggaaaaata gtttaaatgt
      421 taaatcgaaa ggattgtata tgtcaaaagc aaagaaaata tgtttcatta ttttctgtat
      481 tttaatcttg acaattttcc ttcctgtttt gatagattat catcaagtta gtgatctagg
      541 tattcatcta cttagctgga gacagaactc cgtagttgaa ttctatcttg ctagatatgt
      601 cttttggggg acagtggttc tatcaacttt agttttatta tccattttag ttgtgatgtt
      661 ttatcctaaa cgttacttgg aaatccaact tgaaactaaa aacgatacat taaaattaaa
      721 gaattcggca atcgaaggtt ttgttagaag tttggtgagt gatcatagat tgatcaagaa
      781 cccaactgtt catgtaaatt tacgaaaaaa taaatgtttc gttcatgtag aaggtaaaat
      841 tcttccttca gacaacatcg ctgacagatg ccaaataatt caaaatgaaa taactaatgg
      901 attgaagcag ttttttggta ttgagcgtca agtaaaactt gaagttgcag taaaaaatta
      961 ccaaccaaaa cctcaaaaca aaaagactgt tagtcgtgtg aagtaaggaa gtaaaaaatg
     1021 gaatggctta aacaatatcg atatccaatt atcgctggtc tcataggcgt atttctggct
     1081 tgtttgattg tctcctttgg cttcttcaaa acaatatttg tattgatttt aggagcactg
     1141 ggagttgcag ctggattata tatcgaaaaa aactatatag ataaataaaa aaataaaaat
     1201 tactaattta attaaaggag tttcatatgt caaacgaaaa aaacacaaac actaacgtag
     1261 aaaagaaaga tgctactgtt gtagctcacg aaatcaaagg ggaacttact tacgaagata
     1321 aagttatcca aaaaatcatt ggtctttcac tagaaaacgt ttcaggtctt ttgggaatcg
     1381 atggtggttt cttctcaaat cttaaagaaa aaatcgttaa cagcgatgac gtaacaagtg
     1441 gtgttaacgt agaagttggt aaaacacaag ttgcagttga cttaaacgtt attgttgagt
     1501 accaaaaaaa tgttccagct ttatattcag aaatcagaga aatcgtatct tcagaagttg
     1561 ctaaaatgac tgacttggaa attgttgaaa tcaacgtaaa cgttgtcgac atcaaaacta
     1621 aagaacagca tgaagcagac tcagtaagcc ttcaagatcg cgtatctgac gttgctgaat
     1681 caacaggaga attcacttca gaacaattcg aaaaagctaa atctggtctt ggatctggtt
     1741 tctcaactgt tcaagaaaaa gttagcgaag gtgtagaagc tgttaaaggt gcagcaaatg
     1801 gtgtagtatc tcacgaaaac actcgtgtaa actaagataa aataaatata acaggagaaa
     1861 ttatcatgtc agtagaagaa aaattaaatc aagctaaagg ttctattaaa gaaggtgttg
     1921 ggaaagccat cggtgatgaa aaaatggaaa aagaaggagc agctgaaaaa gttgtttcta
     1981 aagtaaaaga agtcgccgaa gatgccaaag acgctgtaga aggtgctgta gaaggtgtta
     2041 aaaacatgtt gagtggcgac gataaataag gttaaaagtt actttatctt tttagtaaca
     2101 ttagtcaaaa gagtctgagt caagatgatt ctcagaaaac aaaaagctag ggattcccaa
     2161 ttgcggaact ctagcttttt aattttgcct ctttctctta ttatatttca gcaggttgtt
     2221 ggccatgagt acgaatccca tgtcaattct cacttgacgc ttccctctca gatgacatct
     2281 cttataaccc aaacaaacct ttatctgccc aaagacagat ttcatatcaa tcttacgttt
     2341 agcgaaaatt tgtctaccct tggaagataa aagtgcctga tattctttag tttttaaaca
     2401 ctggtaacgt tcattcatat acagtccctt ttgaggggct gattcaggtt cataatcgca
     2461 gtcaacattg atttcaaggc tgtttgcttt ctatctaccc tttttcatga tatattttcg
     2521 tacaggttct tcaatagttt ctatagtaaa atgaaccaaa aatagtacac gttgcggtat
     2581 aatgttcttg tggcatattc attagatttt cgtaaacagt tctctcctat ttgtgagcga
     2641 acaggtatag tcaattgatt gtcagtttga agaccaagag tcgtaaggca aaggatatgg
     2701 cagaatccat ccaagggtgg ctggcacaat ttctagtgaa cctctttaaa tctattacgt
     2761 ttgactgtgg aaaagagttt tcaaaatgga aagatatttc caatcatcac gatagtgaga
     2821 gcttttttgc gaatctagga tgttcaagac agcggtgtct caacgaacat tcaaatagac
     2881 ttttaaggtg tcatgacttg ccaaagcaga cagatttcaa cgaagtcagt caagaatttt
     2941 aggctggaca aaaactcaat ttcaggagaa aatgaaataa atattcccac aataaaacgc
     3001 atagtatcaa agtttttcca acacctgata ttatgcattt tttaatttta aagacttttt
     3061 cccagcctca agaaatcaca tcccacctaa atcctttgga taccaaatac ctcatcagat
     3121 attttctgag ttggtaagac tgtctagctt aatttgacaa atggagggcg ttaaatccag
     3181 tttttacagg aagtctattt aaatattttc acactgacct tacaaaatat gaaataaatt
     3241 atataaagtc attcccatga caaagaataa catcccttta aaacagtatc ttacccaatt
     3301 ttcaggtaag acttttgata caaccgtccc caatacccct ccgcatatag aagcaagaaa
     3361 aataactgga acaggtgcta aatgatactg atttaaacct gtaaccacaa caattgtagc
     3421 caaatgagac agctgggaaa agaaaataat tgcaagagaa tacatcgttg cttcttttat
     3481 cgaaatagaa aaaaacacca tcaataaaga tacattgagc ggaccaccac caatccctaa
     3541 aaaactagaa attaagccca atagaagacc acaaatcata tagaatccta tcttatcaaa
     3601 ttgataagtt tttttaaaat ctgttaaaac aaatgataca agtagcatca caaatagtaa
     3661 aatcatttga acaatggata ctagccgtac tgataattga gataacaaaa cttgaaaaat
     3721 aagatgacct agaaatcctc ctaaaacaga agaaaaggat accgtaaaaa tcaatctcca
     3781 ttttatctct ttactttgtg aaaatctttt gattgttgaa ataatcgcca ttataaaaac
     3841 agagaaacta gaataaaaag caatatcact gaccgaatgg tacccaaaac tatccataag
     3901 tggcttaatt ataattccac cacccatgcc tgatatagaa cccaatccac tagctattat
     3961 aatgattaga aaataaataa tctcaaccac tttttaagtc catcctattc tttcaaacat
     4021 ctaaaaccta gatggctact agtggaagta gaaatacatc catttctagc agctactcgg
     4081 tatcgattac aataagaaca atgacataaa aaggaacctc ctctgatagc atactcctca
     4141 tcttgtatcc catattttgg caactcatag tcatcttcat taaaactagc taataaggta
     4201 tatctcggat tgcgacacca ttcccaaaca ttcccaatca tttgatatag accaaaatca
     4261 ttaggtaaaa attcatagac aggagccgtt cctataaatc catctgaggc agtattttca
     4321 taaggaaatc ttccttgcca agtattggca tgataatatc caccttctaa aagagtatct
     4381 ccccaaggat attcagattg tcttcctcct ctagcagcat attcccactg tgcttcagtc
     4441 ggtaaagaca ttccgctcca attacagaaa gccaatgcat cttctaaagc aacatgaact
     4501 actggatggt cttctaaacc cactagatta ctattctcac cgtagggatg tttccagcaa
     4561 gcgttcggaa cttgtaacca ccacggagcc ccagcaggat gaggatatcc ctccctttct
     4621 gcctctggca ttaatatggt ttcctggaat cacgttccca atagttacca tcagattttt
     4681 accatcaaat gctagattat caattccttg taagatttga tctaatttca catctcctac
     4741 atttgccaaa gggaaactct ttgtaactgt acttccaatc gaaatgacaa acgaagcaag
     4801 tgataccaaa ccagcagaaa ctgtatcaac cttgtaaatc ttagcgatat tcactcccaa
     4861 gcaatagatg aacaacaagg aaacaattgg tatacttccc ttgaatacca aattattgat
     4921 gtcaacaagc cactgaaagg ttttcgtaat actttctagg tgaaattgtt gtggtaaatc
     4981 cactagaaaa gcatttaata acaaagcaat ggaacctgtc ataataacag gcatagtccc
     5041 cacaaatgaa tcacgtaaag caactaaaaa cttctgattc ccaactttca ttgcaattgg
     5101 agaaagtttt tcttgcattt tatccaacat tgtattcata ccaaactcct aatttttttc
     5161 aaatcgttcg tatacttcta ccacttcttt cgccaagtca acaaaagtca aactcgtcat
     5221 taaatgatct tgcgcgtgta ccatcaagag gctaattgat gttttttccc cctgtgcctc
     5281 tcttgtcaac atttctgttt gagccttatg cgcctgtaat aaggcagcat tcgcatcagc
     5341 taatcttcga ttggctttag agaaatcccc tttttttgct gcctgaatag cctccatagc
     5401 attacttttg gcttccccac catacataat tagccccatt acagattcta aattactttc
     5461 atccataaca ttctcctcaa tcttagtcta ataaactttc agctaaatct aaaactttag
     5521 aaccattcat cattccgtaa tctgtcatcg gaataaccgc cacaggaatc tgtctatctt
     5581 ttagtttttc ttgaaagtcc cctagtaaat agcgaacttg aggacctaaa agcaacacat
     5641 ttacttcttt tgttgctaca atttcttctg cttcaggagc tggaactgca aaaatttctg
     5701 catccaaccc cttatcttct gctgcctttt gcatctttgt cactagcaga cttgtactca
     5761 tacctgctgc acatgctaac ataattgtta ctttagccat agcttactcc tttatcacct
     5821 atttactgac ctagccaagc gagatactgt atgtctcact cctctaattg ccttgctcaa
     5881 tgcataaata ttatcgactg ttgccagtcc cccatatcct gcgtcaccta tatgttgaat
     5941 atcaactcca caaattttat ttctaagtgc aatttccttg atagtatctg tatcagatgt
     6001 ttcttggcta gtaccaatag cactcaatac aagaccaccc ttactatgaa cgagatcaac
     6061 gacttcacgc aactcttggt catgaaaggc tggaacggtt ccaactgctg gaacaagtat
     6121 cacatccgca cctgcttcca acaattgctc tgcgacagaa agctctgcca caggctcatt
     6181 cactcctgcc ccgtgcattt taccagctat aatcaaacct gagaaattct ctttagcaga
     6241 ttgaactgct ttgataatct cttgattgct aactcctgtt ccaggatttc cagtcaaaca
     6301 gacaaagtca aaacctaact cctctattcg cttcaatgtt tcaacactag caacacgacc
     6361 tgcaacaatt tcctgtgttt cctctagcat ctttgcagat gggtcaatcg gttccaaatt
     6421 tacaccaatt ggacaagcaa caagctggtg taagcgatga ataacatcat cacctgaact
     6481 atccaaagca taaattttag gctcaaaaac atcaacacaa ttcaataaaa tcatatcagc
     6541 tccaaaagct ctagcaattt cagaattagt tatatcccca acaaatgtct cacgagtaac
     6601 tacattttca gatagaacaa ctctaccttc acttgctaaa atactttgtt ttaattcttc
     6661 agcattcatc tgtaatatct cagatggatt tgcactaatt aaacgtttca ttctcaatcc
     6721 tccaataact taaaccgttt ttcaatagct attacaaatg gtaaataaag taagacatcc
     6781 aaaacaatca atacaatttg taataatgaa ccagaaatac ttcctgtagc aagtaaacca
     6841 cttataacag gtggcatagt ccaacctgga tctgtataag ttagcggaac caaacctgat
     6901 gccattgctc cccacgctac tagtaagtta aacattggag ctaagataaa tggaactagt
     6961 aacaaaatgt tcaaaacaat aggaacgcca aacattgctg gttcatttat attaaataat
     7021 ccaggtataa cagttatagg tgccaaagtt tttagttgtt tactagcttt cttcttatgt
     7081 gctaaatatc ctaacgcaag aactaaacct atggttgcac caccgccacc tatgaataca
     7141 aaattatcca taaatacgct agtaaaaata tttggaagag tttctccagt ttgataagct
     7201 actttatttg catctagatt agctaaccat aacggtttca taactgcatt aacaacattt
     7261 cctccgtgta atcctacaaa ccaaaaacta ctattaagga aaattaagat aagaagacca
     7321 ataacattat ttcctaaaag tccaagtgga cctcccaaag ctacttgagc aatttcatga
     7381 agattaggta aacttaattt atctaaagtt gcataaacaa tcaaccaacc aacaatagtc
     7441 actgcaccag gaataatggc actaaagctt ttagatactg ctggtggaac accatccggc
     7501 atttttatct gtatattatg atttataaac cattggtaga tatagccatt aatcaatcct
     7561 aaaatcatag caacaaataa accttttgat gccatgaaag cggtaggcat tcctgctcca
     7621 gcctctcctc taatgaaagg cgtaactgta ataaaagatg ataatgacac aataccagca
     7681 ggaataccat caactttgta ttgtctagcc ataaagtaag cgataccaaa actagctaca
     7741 agaccaagta atccaaaact actatcaaca cccttccata aatagtcagc aacaccaata
     7801 tcacccagat atttttccca tcctggaata ggaaaacttg caataaccat tagtaatgaa
     7861 cctatcaaca ataaaggtat agttagagta ataccgtctc gaatagaaac caacgcttta
     7921 ttattaccca atctagccgc tactggaagt aacttttctt gtaataattt ttccataacc
     7981 ttttcctctt aaatatattt tattatcaaa agttatcaat tatctttatt ttttgacgtc
     8041 aagttgttat ttgattatct tttgttattt atattttaca actataagaa agccttgtca
     8101 aggatttgtg taaacatttg attatttttt attatcaata tataataaaa agagactagt
     8161 aagtaatgaa aagcttaaaa agattaacat ctacatttat ccatagcaaa aaatcatagg
     8221 attttaagaa tctgacaact aggaagaaaa ctaatatgac cttatctaag aaacaattac
     8281 aactaagagc taaaatactg gaaacagttt acacattagg tcccatttca cgaattgaaa
     8341 tcgctactaa aacaggaata acacctgcaa caactagcag tatcacaaat gatctgatta
     8401 aagaaaatat tctcttagaa cttggtgaag acgaacacga tacaagcgta gggagaaaaa
     8461 aaatcctgct agacattcaa gctaaacgat tttactacat tggttgtgaa ctatctgaaa
     8521 aacatttcac ttttgcttta ggagataatc taggaaatat cttaaaggaa gagaaagaaa
     8581 ttgttactaa acagttaatc caagaaaagg gaaatcaact cattaaccag acactaaagc
     8641 aattcttaaa taactgctct gattatgaaa tagaagcaat tggaatcgcg ctacctggtc
     8701 gctatttaga taattataaa ataacaacaa acaatccttt atggcaacat atcgacttag
     8761 aaatgattca atcccacttt gataagcctt tgtttttctc taacaatgtt aactgtatgg
     8821 ctataggtaa acgcctattt agtcgccaac agaatgaccc taattttgct tattttcatt
     8881 ttgctagagg tatgcactgt tcttatatct acgacggaaa catttatggg aaaggaaatc
     8941 taatgattgg agaaataggg catacagttg tttcatctga gggagaagaa tgtagctgcg
     9001 ggcgcaaggg atgcttacaa acttttgctg gagaatcttg gttaatcaaa aaatcaaaaa
     9061 ttctatatca tcaatctccc tattctctac ttcctagttt agtcaaaaat gccgatgata
     9121 ttgatatcca agttatttta accgcttatc aattaggtga cactggaata atcacactta
     9181 tccatcaagc cttactctat cttagtcaaa ccattttaaa tatcagtatg atgattgact
     9241 ctcaaaaaat ctacctacat agtcctttac taacaaatca acacatcatc caaaaactat
     9301 atagtgaaat gaactacaaa cccaaactgc tctataatcg cctacctgaa gtcattattg
     9361 aaccttacaa tgattttaca gccgcacact cagctattgc actttgtttg tatcatacaa
     9421 ttctacattc ttaattttct attacattta ataaataggc taaaaagagg gagtatatca
     9481 taaaaagcga ataaacctgt tttctgaaaa tcagaattct gttttgttcg ctttctttat
     9541 atttggaatt atactcctat ctcccctttt tcataatgta taataaaatg aaataataac
     9601 aggacgaatc gatcgggaca gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt
     9661 actattctag tttcaatcta ctatattttc gtacaggtgc ttcaaccatt tgaacgattt
     9721 caaatccttc tttttggtaa agattctgaa ctctttgatt tgcctcgaag acatttagag
     9781 aaatactatc tatatcttca ttttcaaagg ccaaactaac aaatttcctt aaagcctggc
     9841 tacctaagtc ttgctcctgt ttctgggggt tgataaaaaa tctcccgata tgaagattgc
     9901 tgtcttctag cctgattttc tggataaatc ccacaaactc ttgttcatca aagattgaaa
     9961 agactccttc caaggcttga agggtcagta gaaaaggaat ccttggtccc atccattgtt
    10021 cttgaaagga tttgcctagg gagttggacc actggcatac aaattgagcg ttttctgtgc
    10081 tcaccttttc ttcaaaacga attgtcatct tttcctcacc accttatcta tgtttctcca
    10141 ttatactatt tctcccattt tttacgaata gataagtatg attgattttt atttttttct
    10201 cgtcgggagc attctagctt cctttcttgg tttggtcatt gaccgttttc cagagcaatc
    10261 cattatcagt tcagccagtc actgcgattc ctgtcagact cgcttgcgtc ccttagattt
    10321 gattccgatt ctctcacagg tcttcaatcg cttttgctgt cgctactgca aggtccgcta
    10381 tcctgtctgg tatgccctct ttgaactagg cttaggactc ctctttctgc tttactcttg
    10441 ggaattactt tccttgagta aagtcatcct aatcactgct ggtttgacct tgggcatcta
    10501 cgactttcgc catcaggaat atcccttact ggtctggatg actttccacc taatcctaat
    10561 agcttcctct ggctggaatc tggtcatggt ctccttcctc atacttggaa ttttggctca
    10621 ttttatcgat atccgcatgg gtgcagggga tttccttttt ttagcttctt gcgctctcgt
    10681 ctttagcgta acggagttac tgatcttgat tcagttcgct tctgcgacgg gtatcctggc
    10741 ctttctcctg caaaagaaaa aggaaagact tcctttcgtg cctttcctct tacttgctgc
    10801 ttgtgtgatt atttttggta agctactgct tgtttgataa agtccaattt ctgccatatg
    10861 tccttcatga aattatttca cagttaaatt ataaattatt tcttttgtac aaagggatga
    10921 tgttatcaaa tcgatctgtt cttctatctt cttgatactg atcaaaaaat ttcatttcga
    10981 ctgaaaatat ttcgcttata aactgtaaac gaatactttg tttagacatt atagtcgcta
    11041 gactgactag atgattactc aaaacgacgt ccagaatact ctttactttg cttggttttt
    11101 taacaaaaat ttgatcatcc aaaggttcaa tcattttgta acctttttgc gcaatttgac
    11161 gataaaagta agaatgttgc tttggagtca ataatcctaa cttaaaagct cgatactcta
    11221 aagcctgtat cgaaacattc aaatccgact tcaataaaat ataactatca ggattgctga
    11281 cacgcttgcc aaccctctct tcaaatttga ctaaaaactc ttcttttggc aataaaaaac
    11341 atgatgcaaa ataatttgct tcttgctcca aacgatcgcc atcttcattc atatctttat
    11401 atttatgtaa aagaatatgt cctagctcat gagctaagtc aaaatttcga cgtacagatg
    11461 atttattcgt tcccaacaca atataaggtc ttcccaattt tgaccatgcg ctataagcat
    11521 cagcttggcc attaattaat cgttccacga tatagatgcc tgaacgttct aatttataaa
    11581 gcaaatcatg attatctttt gaaataccta atttttccct ggcataaaga gccaattcct
    11641 caatggattc tcccttatga taagattcac tcactacatt acttaggtca tgaattataa
    11701 tattaggtat aattacaaaa ctttcaaaat aatcaatcaa actatctacc ttatgtaaat
    11761 acatagtttg aatatctatt gttttccgtg ttgctaggtc tgcatttcta aaggcaatta
    11821 cagaagaatc aaatcgaatg ctctcttctt cctgttcaaa ataagttaaa tcaacatgaa
    11881 attggttggc caaatgcatt ttggttgata atttaggttt cgtttcgttg gactcaaact
    11941 gccaaatggc ttgttccgtt aaattaattc tctgagctaa ttctgctcta cttaaaccat
    12001 ttaacagccg taattctttc aatacccgac cattaaacat ttacatactc cttactactt
    12061 ttgaccttct tgtttttcta ttcttggaat aatttcaaaa tcttctgttt ccgataattc
    12121 tgaaaaatta ggaatatctt gatatttagc ttcttcgaaa tggtacgaac ttgattgaat
    12181 atactcagac aaatcttgaa tcaagtgtaa ctgaccagtt cgtgcatcag gcataacaac
    12241 atctacagat ataatattgt tttctgagtc cgcctcataa gttaaaatca taaatttttc
    12301 gatattcgaa tttttagtag cttgttcaat ttcttgaatc atttcatcag aaactaactc
    12361 catctgaatt ggaaaggaat gactatcttc atcatttttg taggaagaat gttgattaag
    12421 ataaagtgta ttcatctgag cgtattcaaa taagtagcca ctcttatttt tttgtaccaa
    12481 aggaaattgg ttcgtaagtc gcttcttacc ctttataatt aacaatactt tcccatattt
    12541 ttctgtattt gtttcaaatt ctaaatatcc ccaagtctgt cctgctaatt gtaatttata
    12601 ctcaaacaaa tctgctgatg caaatgcagt atcaatatga ttaggtcgcg tccatgcata
    12661 accattcgac actatcattg tctctctttt ttctagacgt tcatctacat aatctttttg
    12721 ccctttcatc aaagtatcta caattttttg tgcctcaagc gaatcaaaga gatcctgatt
    12781 caacataatt cttcctcctc caaatacttt ttaatgaatt atatcatttt cttaaagaaa
    12841 ttactacaat aattagcttt ttcttaaagt tctgtgtcag agtaatttag aaaattatat
    12901 cttctatagt aaaatcaatt aaaaactgaa caaatttatt gggaaattca aatcactttc
    12961 ttaaaatatt ttaggaaccg tagtgtaata ttccagattc aattcactat aaaactagcc
    13021 tttctcctgc aaaagaaaaa ggaaagactt cctttcgtgc ctttcctctt acttgctact
    13081 tgtttgatta tttttggtaa gctactgctt gtctgataaa atcctgaatc ggctctcctt
    13141 ggtggagagc ttttactatt ttcgaaccga cgataacacc atctgacacc gcattgaagc
    13201 gttccagatc ggcttgacta gatacaccaa aacctgtcaa gactgggatg tcggccactt
    13261 gatgaagttg cgccaagtgc ttgtccaaat ctgcacggta attgcctgat ttccctgtca
    13321 ccccattgat ggcaacggca tagacgaatc cctccgcgcc ttcaatcaac tctttctggc
    13381 gctcaattcc tgtggtcaag cttactaaag gaatcaaggc gatatctgta tctgccaaaa
    13441 atggttctac aaagttggca tgttcatgag gcaagtctgg gataatcaag cccttcacag
    13501 ctgtatcagc cagatctttg acaaagttct ccacaccgta ctgaaagagg gggttgaagt
    13561 aggtcatgat gaccagtgga atctctgttt caatggtttt caaggtttca actaaagcct
    13621 gggtagaggt cccgtgggct aaactgcgca agccagcttc ttcgataaca ggtccatctg
    13681 caacagggtc tgaaaaggga atacccactt caatagcaga gacacccaaa tcttctaaaa
    13741 agtgaattgt ttcagcaaga ccgtccaaac ctttctcgtg gtcaccagcc atgatatagg
    13801 gaacaaaaat tccttttcca gttgctttta tagcattcaa tttttctgtt agtgtcttag
    13861 gcatgagctt ctcccttctt tgctgcatct gcttccaagc ggtctttgac ttgaaccaca
    13921 tccttgtccc cacgacctga taggcagaca atcatagact tttctggtcc aagttctttg
    13981 gccaatttca ccgcaaaggc gatagcatgg ctagattcca aggctgggat aatcccttcc
    14041 acacgagaca agagttggaa tccttccaag gcttcttcgt ctgtcacagg gacatagctg
    14101 gcacgtttaa tatcgtggta gtgagaatgc tctggaccga taccaggata gtccaaacct
    14161 gctgagatag agaaggcttc aagaatttga ccatgggcat cttggagcac atccatgagg
    14221 gaaccgtgaa ggacacctgg acgacccttg gtcaaggtag ctgcgtggtg ctccgtatcc
    14281 acaccaagtc cagccgcttc agctccatac atggctactg actcatcttc tacaaaggga
    14341 tggaagagac cgatggcatt cgacccacca ccaacacagg ctactagggc atctggcaga
    14401 ttttgtcctg tcaagtcacg gtactgttgt ttagcctctc gaccaatgac actttggaag
    14461 tcacgaacaa tttctggaaa tggatgaggc cccaaggcag aaccaaggat atagtgggta
    14521 tcgtcgatat tagccaccca tgaacgaagg gctgcattga ccgcatcctt gagcacgcgc
    14581 gaaccatctg tcacggcctc gaccttagct cccaaaagct ccatacggaa gacattgagg
    14641 gcttggcgtt tgacatcttc ctcacccatg tagatggtac attccatgtt aaagagggcc
    14701 gcagcagttg cagttgccac accgtgctga ccagcacccg tttctgcgat aattttcttt
    14761 ttacccatgc gtttggcaag ccaaacttgt cctaaggcat tgttaatctt gtgggctcct
    14821 gtatggttaa ggtcttcccg tttgagataa atcttggctc cgccgatatg ctgggtcaag
    14881 ttttttgcgt aataaagagg agtttcacgt cctacgtact ggcgcaaaag ctggtttaat
    14941 tcctcttgga aacttgggtc tgcctgactt tcacggtagg ccttctccaa ctccaaaact
    15001 gctgtcatca atgtttctgg gacaaaacgt ccgccgaatt ttccgtaaaa tccatcttta
    15061 tttggttcct gatatgccat gctttaccct ctctataaat cttctaatct tttcatgatc
    15121 tttttgtcca tctgtctcca ctccgctcga tacatctact gcatagggag taaaatgttg
    15181 aattgctttt actacattat cttcattaag gccacctgcg ataaagaagg gctgtgctag
    15241 tccagtcgta tccagttgac cccaatcaaa gggctggcca cttcctgcca caggggcatc
    15301 aaagagtaga taatctgcct gagaattggg gacatgccca tttccatcta cctgcacagc
    15361 ctgaatactg gcacaaggca aattctcaaa taaatcatct gccacctgac cgtgaacttg
    15421 aaccaagtcc aagccaactt tgtcaatcgc ttccagcagt tctacccgac ttggtgaaac
    15481 aaatactcca acctttttca catctgcagg aataagcttt gccaacacag ctgcctcttc
    15541 taaagtcacc tgtcttttac taggtgcaaa gacaaaaccg atatagtcgg ctcctgctga
    15601 aacggctgtt tccaccgctt ctttggtcga tagtccacaa attttaacct ttgtcaatct
    15661 gcaactcctt gattctctgg gccacatttt ctgcctgcat aagagctgtc cctaccaaaa
    15721 ttccgttaaa gtatggggct agtcgttccg catcctgccc tgtgaaaatg gcagattcag
    15781 aaatgtaata gcgaccttcc tcaaagtaag gggctaaatc tacactggtc tgcaagtcga
    15841 cctcaaaggt agtcaagttg cggttgttga ccccgataat ctcagcacca agtctgtggg
    15901 ctacctctag ttcagctaga ttgtgagtct ccactaagac ttccagacca agctctgtcg
    15961 cgtagtcata cagttccttg aggcgttctt cggacaaggc tgccacaata agcaagataa
    16021 ctgtcgcacc tgcattgcga gcgcggatga tttgcttttc atctatgata aagtctttgt
    16081 tgagcgtcgg aatctctacc tgactggaaa tttcccgtag ataatccaaa tgccctttaa
    16141 agaaaacctc atctgtcaac accgaaatca tcactgctcc gttttcttca taagtctggg
    16201 cctgttgcac aatatccaca tcgagattga tatctcccaa actagggcta gctttcttga
    16261 cctcagcgat tacctgcaag cggtcctgat gattcttcaa aaattctgcc aagcgatagg
    16321 tctggcgcag aggctggatt tgctccagct tcatctgctc cacctcacgc gccttctgct
    16381 ccaagattcg tgctaaaaat tcctgactca tttttggtac tcctgtaaca gtctgagttt
    16441 ttcaagggcc ttgcctctag caatcacttg acgggccaag gcaactcctt ccttgatgct
    16501 atcaatctta ccattagcat agaaaccaag accagcattc aagactgtcg tttccaagaa
    16561 tggacttgct tcgtttttca gaacgctaag caaaatttct gcattttcct gagcattccc
    16621 accacgaata tcttccatag catagccttc cattcccaaa tcctctggag taaagcttga
    16681 caagctgatt tcgccatttt caagaagtgc aatcttggtt gttccgttca agccagcttc
    16741 atccaaccct tctggtccag caaccacgat ggcacgtttg cgacccatat ttttcaaaac
    16801 ctgagctgta ctttctagga gttctggacg actaattcca agaagctgtg tttctaaagc
    16861 cattggatga atcagtggac cagtcaagtt cataatcgtt ggaattccca attccaaacg
    16921 agctggcatg atgtatttca tagctgggtg catattttta gcgaagagaa agacgatgcc
    16981 agttttatca aagaccttac ctagttcagc tggtttgagg tcaagattga ttcccaaggc
    17041 ttcgaggaca tctgcggaac cagatttaga agaaatcgag cggttaccat gcttggccat
    17101 gtgaataccg ccaccagcca agacaaaggc tgcagttgtg gaaatattaa aactgaaaga
    17161 cttgtcccca cctgtaccac agttgtccat agcatcatga atctcagttg gaatatgctg
    17221 ggcatgtcct ctcatgactc gggcaatggc tgtgcgttct tcaggtgttt cccccttcat
    17281 cttaagagct aagaggagag aagcaatctg cgcttcagtt acacgcccag ttacgatacg
    17341 ctcaatgaca tccgtcattt ccacacctga taaattttca aattttgcta gtttttcaat
    17401 aatctctttc atcctagttt cctcacttta caaccttctc gataaaattc cgaatagaag
    17461 acaagccgtc tggcgttcca atgctctctg gatggtactg gaagccataa atcggtaggt
    17521 ttttatgttg aatccccatg atggcttggt catcagtcga acgagctgtc acttcaaagt
    17581 cttctggcat ttcctcaatc aaaatactgt gataacgcat gaccgcacgg tcatcctcaa
    17641 taccttgata caaaacagat ggcgcttcaa agttgatatt gctctgtttc ccatgcatga
    17701 cttttggagc caaacctagc ttaccaccaa agacttctgc aatggcttgg tggcctaaac
    17761 aaatccctag aatcggcttc ttgcctgcaa agtcacgaat catgtcttcc atctttccag
    17821 catcaactgg ccaaccagga ccaggagaaa agaccagacc atctgctttt tcagcttctt
    17881 catacagctt ggaatcatca tttctcagaa cctgaacttc tgcaaaattc ccaatgtatt
    17941 gggccaagtt ataggtaaaa gaatcatagt tgtcaatcaa taaaatcatg gtcttagttc
    18001 tccaattcta gtcatagatt ttgctttgtt aatggtttct tggtattcgt tttgggcgat
    18061 agagtcgtag acaatccctg ccccagcctg cacataggct ctttgatttt tgagaattat
    18121 ggttcggatg gcaatggcca aatccatatc acccgtcgca gacaagtagc cgattgctcc
    18181 tgcgtatacg ccccgttttt ctgtttccag ttcatagata cgtctcatag cacgaatctt
    18241 tggtgctcca gaaacggttc cagcaggaag tgtagctttc aaggcatcca tggcagtgag
    18301 ttctgcaagc aaacgcccct tgaccacgct ggtcagatgc ataacataac ggaagagctc
    18361 cacttccata tacttagtga cttggacact ggtcgtttca gagatgcgac caatatcatt
    18421 acgacccagg tctactaaca ttcgatgctc tgctgtttcc ttctcatcag agaggaggtc
    18481 agtcgccaag gccttgtctt cttcatccgt agcccctctt ggtcgcgtcc ctgcaatcgg
    18541 attggttgtc acgatgccat ttttgacaga aaccaaactt tctggactgg ctccgatgat
    18601 ttgataatcc ccaaaatcat agaaataaag gtaattagaa ggattagtca cgcggagatt
    18661 tctgtagaag tcaaatggat ttccagtaac ctctgctgag aagcgctggc tgagtacgca
    18721 ttggaacata tcaccgttac gaatcaagtc acgagctgtt tctaccattc cctcaaactt
    18781 atgtggagcg atatgcggtt tgaagtcaag tggtgataaa tccaagtctt caaattcatt
    18841 tggagcagga atgcgtaatt cctcaagcac ttggttcaag gatttttcca aggcctcttg
    18901 actgcgctca ctataaagtg catcctctat gacatgtatc ttctccttct tgtggtcaaa
    18961 gaccatatag ctctcataga caaagaaatg catgtctggc gtcccaattg tatcctcagg
    19021 gatttgacca atttcttcat aaagcgaaat catatcgtaa cccacaaaac caatggctcc
    19081 accaccaaaa gggagctctg aatggtgctg actcttatga atcacttcat aaaggaaatc
    19141 caagggatcc cgatcaatca cttgaccatt ttgatagaga actccatttt caaacttaat
    19201 ctcaaaaact ggattatagg ctaggataga aaaacgagcc gtttccttgt ctctcggaat
    19261 actctctaaa ataaccttat gttgcccctt taagcgcata taagccaaga ttggtgataa
    19321 gacatctcca tgaatgattc gttccattgt aatttccctt tcagttctaa ttctagttcg
    19381 tggcgactgt atgaaaaatc cccacgcaaa ataacttgcg tgaggacgaa attcgcggtg
    19441 ccacctcaat tataggattt ctcctatctc tcattcctgt ctcagatacc tcctgtaaca
    19501 ggctgtgcga taaagggcac tcccttgaga atgatgtttt cttctctcgt ttcagatgaa
    19561 cccaacttta cagctttctc tgcttgtttt cagcaaccac aagctctctg tgagagaaag
    19621 aactgtaatt tttccatcta ttatttttta gcttctagta gtctgcaatc gcagctaggt
    19681 ccttgcctcc acgaccagag acattgatga agagatgttc atctcggtac acctttatac
    19741 tcttcgaaaa actcttcaaa ccgcgtcaac gtcgccttgc cgtaggtatg gttactgact
    19801 tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agttctatct
    19861 gcaacctcaa aacagtgttt tgagctgact tcgtcagttc tatccacaac ctcaaaacag
    19921 tattttgagc tgacttcgtc agttctatcc acaacctcaa aacagtattt tgagctgact
    19981 tcgtcagttc tatctgaaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta
    20041 gtttgctctt tgattttcat tgagtattac tagctttttt cgtattagtc cagccttttt
    20101 gtttactttt agtaggaggc aaggagctgt agatagaact caagttcatc aaagcgactt
    20161 aaggccctaa taaaagataa accaaacgac ggatagaaaa aagcccacac acagaatata
    20221 cttccgtgtg agggcgttgg taacgcggtg ccacctcaat tataaaggga ctatcccttt
    20281 acatctctgc cttgtttaac aacaagctgc actgtaaggt gtgcgcaccg aattttcatt
    20341 gtttcaaatt cattttcaaa atcagcccac tttcactact tccaaccacc tattcacaat
    20401 caccacaggc tccctgaaga tcaaaaatag ttacttttct gatttgttga acttatttta
    20461 atactttgtt ttttctttgt caagactttt ttacgatttt tttgaaaata tcattcgaat
    20521 gtgaccatgt cttccttaga tcgaacatga acatgtccca cttcttagaa attggatcca
    20581 actcaataga aactgaatgg aggctaaaca gaacttattt tagaacactc catcttttcc
    20641 actaggattt tcaagaatta aacaatacta gaaactctgt ctcctaacaa atttaggaga
    20701 aacttcaaca gatgtgacac tttccccttt aataattgct aaaacacctt ctatcatttc
    20761 tttagccaat ttaacataat tgggagcaat tgtagacaaa gctggagtat aatactgaga
    20821 aataggaata ttatcaaatc caataataga aatatcatct ggaataagaa ttcctttctc
    20881 atagcacgca cgaatcaagc cctgaacctt ttcatcgcct gaaacaaaaa taatgtccgg
    20941 ataattttgg gtagtcaagt gctgcattgc ataagaataa actgaatcaa ttgtagataa
    21001 gccataaatg acttttaaat ccataaagta atttttatca ttcagaaaag aacgcacacc
    21061 tttttcacga tccttattaa catgggattc tcctcccata agcaaccaca tatttttaaa
    21121 tttttcttca gttacagctt tcatcatatc ataagtagct tgaaaattat tattagatac
    21181 atagactact ccagacgttt gagattcacc gaaaacaaga aaaggcatat ggttcttctt
    21241 taaatactga attctgatat catctacact ttcataaaaa acaataacac catctactag
    21301 gctacctgtg cttgatataa ttgaattact aattgtatcc tcctctccaa agtactcaac
    21361 tatagcatta acaccaaatt ctttacacgt ccgtaacact ttatctaaca gcgtatgaaa
    21421 ccaaattaaa ggaaaagagt cgattttttt tacagaaatc aatatattta tagcttcttt
    21481 tttagttaaa ttttttgcat acgcatttgg aatatacgac aattcctcta taactttttg
    21541 aatcgcttga taagtttctt ctttaacatt tactccacca ttaataactc gtgaaactgt
    21601 ttttggagaa aaacctgata aacgtgcaat atcataaata gttacctttt tcccatttat
    21661 atttttcatt tcagtcctcc a
//
LOCUS       DAVKWS010000031        21663 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKWS010000031 DAVKWS010000000
VERSION     DAVKWS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.031"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..220)
                     /locus_tag="VW852_001289"
     CDS             complement(<1..220)
                     /locus_tag="VW852_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV0272431.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAA"
     gene            complement(601..1461)
                     /locus_tag="VW852_001290"
     CDS             complement(601..1461)
                     /locus_tag="VW852_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV0272432.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1477..2754)
                     /locus_tag="VW852_001291"
     CDS             complement(1477..2754)
                     /locus_tag="VW852_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0272433.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(2747..3298)
                     /locus_tag="VW852_001292"
     CDS             complement(2747..3298)
                     /locus_tag="VW852_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272434.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3314..4573)
                     /locus_tag="VW852_001293"
     CDS             complement(3314..4573)
                     /locus_tag="VW852_001293"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0272435.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            complement(5716..5874)
                     /locus_tag="VW852_001294"
     CDS             complement(5716..5874)
                     /locus_tag="VW852_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272436.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(5884..7194)
                     /gene="obgE"
                     /locus_tag="VW852_001295"
     CDS             complement(5884..7194)
                     /gene="obgE"
                     /locus_tag="VW852_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV0272437.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(7258..7392)
                     /locus_tag="VW852_001296"
     CDS             complement(7258..7392)
                     /locus_tag="VW852_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV0272438.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(7451..7627)
                     /locus_tag="VW852_001297"
     CDS             complement(7451..7627)
                     /locus_tag="VW852_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0272439.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(7658..8983)
                     /locus_tag="VW852_001298"
     CDS             complement(7658..8983)
                     /locus_tag="VW852_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0272440.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRSSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(8999..10042)
                     /gene="cpoA"
                     /locus_tag="VW852_001299"
     CDS             complement(8999..10042)
                     /gene="cpoA"
                     /locus_tag="VW852_001299"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV0272441.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            complement(10157..10486)
                     /locus_tag="VW852_001300"
     CDS             complement(10157..10486)
                     /locus_tag="VW852_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0272442.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(10500..11609)
                     /gene="rpoD"
                     /locus_tag="VW852_001301"
     CDS             complement(10500..11609)
                     /gene="rpoD"
                     /locus_tag="VW852_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV0272443.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(11612..13372)
                     /gene="dnaG"
                     /locus_tag="VW852_001302"
     CDS             complement(11612..13372)
                     /gene="dnaG"
                     /locus_tag="VW852_001302"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV0272444.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(13462..14220)
                     /locus_tag="VW852_001303"
     CDS             complement(13462..14220)
                     /locus_tag="VW852_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272445.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(14217..15083)
                     /locus_tag="VW852_001304"
     CDS             complement(14217..15083)
                     /locus_tag="VW852_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272446.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(15152..16156)
                     /locus_tag="VW852_001305"
     CDS             complement(15152..16156)
                     /locus_tag="VW852_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0272447.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(16269..16486)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16569..19265)
                     /gene="ppc"
                     /locus_tag="VW852_001306"
     CDS             complement(16569..19265)
                     /gene="ppc"
                     /locus_tag="VW852_001306"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV0272448.1"
                     /translation="MSLQKLENYSNKSVVKEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWHNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(19285..20514)
                     /locus_tag="VW852_001307"
     CDS             complement(19285..20514)
                     /locus_tag="VW852_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0272449.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(20670..20972)
                     /locus_tag="VW852_001308"
                     /pseudo
     CDS             complement(20670..20972)
                     /locus_tag="VW852_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(21420..21641)
                     /locus_tag="VW852_001309"
     CDS             complement(21420..21641)
                     /locus_tag="VW852_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272450.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTDSAYAKIERGEHTLTADI
                     LVTLSNFYDVSTDYLLGLTDFPDKIRFRK"
BASE COUNT         6441 a         4861 c         3723 g         6638 t
ORIGIN      
        1 cagccgcaat ttgttcataa gttcgatatt ctcgcacata ttgaagagtg gccataagaa
       61 ggtcttctag gcttaattta ggttttcgtc caccttttgc gtgtttaagt tgataagctg
      121 tttttaatac agctaatatc tcttcaaaag tcgtgcgctg aacaccaaca agacgcttaa
      181 atcgtgcatc agttagttgt ttacttgctt catcattcat agaactacta taccatattt
      241 tatttcgcag gaagtctaat ctaaaaaagg taaatttaag attggcaata cgaacgttaa
      301 acagaattcc atttctcttt ttagaatcaa aacgacaata ttgtggaatc tctacaggaa
      361 caatagactg cgacactaaa atatcctctc ttagttttac tttactaata gtatatagga
      421 ggagagccct gaatcctaga ccctttgtta tgatgcgctt acaaacagag ttggaaaaaa
      481 tcttaaaagc agaaaaacgc atgatatcag cccctaacac ttgatacaat gcgttttatt
      541 atgaaaagat ctactggatt ttttatcaaa ataaacttcc agtagtcttt tcacttttta
      601 ttaataagtt ccttcttcac cttggctagt caagataaca ggtccatctt tcgtaatgac
      661 aaattggtgt tcatactgac atgacaatcc accgtcaatg gtcttatgcg cccaaccagt
      721 tttcatatct gtatcaattt cccaatcgcc tgtattgatc attggttcaa tggttaagac
      781 cattccttca cgaagacgga gtccacgacc tgcaatacca tagttaggaa ccattggttc
      841 ttcgtgcata gttgggccaa caccatgacc aaccaaatca cgcactacac cgtaaccacg
      901 actttcagcg tattcttgaa tagccgcacc gatatcaccg atacgatttc caacaacagc
      961 ttgctcaata cccttgtaca tagcttcttt ggttacatcc atcaagtttt tgacttcttc
     1021 ggacggtgta ccaacagcat aagcccaaca tgagtctgct aaaccaccag aatagctctg
     1081 agtgtatttt ttcatttgtt caacattgtt gaagtttaat tttgagacat ttaggtcaga
     1141 tttagcaatg ggacctccca aaaccatatc aactttgagc aaatcaccat ctttcaagat
     1201 ataatgacga gggaaagcgt gagccacttc atcgttaaga gagcaacagg tagcataagg
     1261 atagtccatc atggcaccgt caaccccaat ctgaagtgga aggaaatttt cttctttaca
     1321 acgacggcgg acatattctt caacttccca catatctacg cctggcttaa tcaaatcacg
     1381 taagcctata tgaatacttg ctagaaaatc accagccttg tccatagctt cgatttcacg
     1441 agctgatttt aatgttatca tcttttctcc tagtttctaa ttaattttaa cagtcacatt
     1501 tgcttttgaa acaatctggt gaccatgata aatatcgtaa tcaattatag ctgaccgtct
     1561 cgtatgatga ataatccgtg cctgaatgcg caatatatca tctatctgaa cagcctgcaa
     1621 aaagtagatc agcatctgct cgataatgag attgcgacca ctattaacaa ctaaatcttg
     1681 ggtcatgtgg gtcagaattt ctgccaatac accattagcc aaaactccat ttttttctag
     1741 cataaagggt tccactgtaa tgactacttc atcatggtga taagagagct tttgtccaat
     1801 ctgctcagaa aaagttggta gagccgaaac ttgggaacgg ctcatcttct ccatgacatc
     1861 tcgtcgcgtc acaacgccaa gcaaagtttg attgcttcga acaactggta ccatttcaaa
     1921 gtcttctgcg atcatccgtt gactcacatt ggcaatattt gtcgataatc caaccaaaaa
     1981 tagactacga gacataacct tatcaattgt cgtgcttggt gatttatcac cagcgtctct
     2041 catggttaca acaccaacaa cgacctgatg ttgattgata acagggaaac ggctgctacg
     2101 attcttacga accaagtcca aataatcttt aactgtatct gtctctctca gaaaaccata
     2161 ctcatgacta gggcgataaa gtttctcaac tgtcagaata tcagtcttga tttggacatt
     2221 tgacaaggct ttattgatca tggtcgcgac ggtaaaggta tcatgcttac ttcttagaac
     2281 aggaatccct ttttgattgg ccagtttaag cacatcatca tgaacctgaa atccccctgt
     2341 aaccagaact gcattttcat tttccaaggc tagcaactga atacgggttc ggtctccgac
     2401 aatcaagagc cccccatcat gaaggtaaga caagatattt tgttcagtca tggcaccaat
     2461 tgaaaactta ctaaactctc tctctaaacc ttcttgccca gccagaacct cagaagaagt
     2521 cacttctgca atttcagcaa acgttaatct ctctatagca actttctggg atttaacacg
     2581 aattgttcca cttctaggac gggtctccac aattccacgg ttttcagctt ctttaatagc
     2641 ccgataggct gttccatcac taactcctag atgattcgaa atgctacgaa cactgaccct
     2701 tttacctact ggtaattcct ccaaatagct tagaatttcc tgatgcttac tcattgaact
     2761 ctccttttac ataccgaaat tcaagataat cccgcatttt tcttgtgtaa cgatcacttc
     2821 ccttaaactg acggaacaaa ctaaatccag acttaagagc aactctttgg ctagctttat
     2881 tttcaaggtg ggtaatgata aataattgtt ttaagccaaa ttcctcaaaa gaaagctgac
     2941 aaatttttct aacaacctct gtcataaatc cttgcgacca agcatctttt ctcaaaaaat
     3001 agccaagctc agcttctttt ttgatttcat ctaacttctc aaatttaata gaaccaatca
     3061 tttgttgatt tttctggtca caaattgccc acactcccaa aggggacttc ataaagtaat
     3121 tggccagtgc atattgactt tcttccagac ttgcctgcgt tgggaaaata aattgaagat
     3181 tttctggatt tgaagctatc tcgcggaagt cctgactatc actaaaaaag aaaggacgca
     3241 aatacaagcg atccgtttca aaaaaagaaa acattgctaa tttggtccaa atattcataa
     3301 acacctctac ggtttaatcc tcaacaagtc taatatccgc tcctaaatta cgtaattttt
     3361 cgataatatc agaataacca cgtaagataa actcgatatt ggtaatttca gttttacctt
     3421 cagccataag cccagcaatg actagtgcag ccccagctct taagtcggtc gctttaacac
     3481 tggccccacg taaatcacgt ccacccgtgt acaaaatatg accatttgtt gtcgaaatat
     3541 ccgcatccat ctttgctagt tcaaaaacat gatttacacg tttttcgtaa atcgtatcga
     3601 caattgtacc acgaccattc gctcttagta aaagaggggt aagcggttgt tgcaaatcag
     3661 ttgcaaagcc tgggtaagga gctgtcttaa tattgattgc tttcaaatta gactgttcct
     3721 cgacaaaaat gctgtcttca gatacagtca ttctcactcc catttcttcc aacttagcaa
     3781 taaacccttc caggtgttcg taaagaacat tatttatacg aattccttta ccaactgcag
     3841 cagctaaaga tatatatgtt ccagcttcaa tgcggtctgg aatcacctga tgacgtgtcc
     3901 catgtaatct ttcaacacca tcaataatga tgatattagt tcctgcccca cggatatggg
     3961 cacccatatt attcaagaga gtagctacat caataatctc aggttcacgg gctgcatttt
     4021 caataatagt acgaccattt gctttaaccg cagcaatcat cgtattaatc gttgctccca
     4081 cactaaccgt atccatgtaa atacttgcac catgaagtcc tgtatcttta gcagataact
     4141 tcatgttatc tccctcgtag ctagcagtgg cacccatagc ttcaaacgcc ttaaggtgta
     4201 agtcaatcgg acgaggacca agatcacatc ctcccggtag accaactgtc gcttcaccaa
     4261 aacggcctaa gaggctccca taaaaatagt aagatgcacg aagactgtta attttaccat
     4321 aaggcattgg aatattttga acacctcttg ggtcaatctc caatacatcg tcataacgct
     4381 taacagtagc tcccatcaat tccatgattt cgacaagact ggctacatcc gaaatatctg
     4441 gaacgcaatc caaagtcacc acatcatcag ccaagataat agctggaatt aaggcaacga
     4501 cactattttt agcaccacta atagtgattt caccttgcag tggtaatcca ccattgataa
     4561 caatttttct cattctaagt tttcaatact ctttcaagat ttctaataag gtcttcaaaa
     4621 taaagtatat cataaattta tccttttttc catccttttc aattttattg gataaatcag
     4681 agttgacgag ttagtaactg aattactaat actcaatgaa aatcaaagat caaactagga
     4741 aactagccgc aggttgctca aaacactgtt ttgaggttgt agatggaact gacgtagtca
     4801 gtatcctaca catacgacaa ggcgatattg acgtgatttg aagagatttt cgaagagtat
     4861 aatctttctc gaatcgctat tgcccctttt ttatccccta aaactgagta ggagctaaga
     4921 ctagatctct tatctaagta tcggaatacg gctgttcaac taaattttag caagaacttc
     4981 tcccccttct ctcagttcac agaagcgttt tatacatacc gtcctatcag acagataacc
     5041 gtgactcagg atcacttcta tctgtcgttt tctctttata gtgatagacc tcacgggata
     5101 agtcgtacat ctttcctcgt gtatacaaga tataaggacg ttaaaaggtt acagtgaaaa
     5161 tagaaaatct tagtacgaat tcaattactc tcgtgattta cgcatatagg ttacgactac
     5221 ctttttggac tttagagttt gagaccccct tatccgctat atacgcctta ttatcacgtt
     5281 cctgttcgta gagccacgat ttcgctatcc cttcttctga caattcttat ttccagcgtc
     5341 agacttaaaa cggtctatcc ccagaccatt ttaatctgct acctcacgat agtcaggctt
     5401 gggaatcgct atttgactcg ccgagaactt tgacatcaga tatactataa caaattgaaa
     5461 taagaatcaa acaatttaat tttgaaattc aaatcaattt ataacaatat agttgattga
     5521 aactagaata gtacatctct gcttctaaaa cattgttaga aatcgatttt actgtcctga
     5581 tcgatttgtc ctgttctcat ttcattttac tatattttag aagatatgct gtgctattcc
     5641 agattcagtt tgctatacat gcctgtcgta ccacaaaatc ccgtgtctga tgaggcacgg
     5701 gattttttag cttctttact gagatggatt ttccttttta gttcgtgcca atctcaaggc
     5761 acgatttgga ttgagacgat taaatagttc ttccaatttc ttttcattcc aaacgtctgc
     5821 ggcggaaaca aaattaccat ccgcatctcg gaaagatatc ggtttatctc ccataccagt
     5881 ctcctagtct acaaattcaa actcaaattt accaatgcgg accaaatccc catctttagc
     5941 tccacgcgca cgaagggctt catcaacccc cataccacga agctgacggg caaatttcat
     6001 gacagattca tcacgatcaa agttggtcat attaaagagt ttcatgagtt tttcaccaga
     6061 aagtacccat gtcgcatcgt catcacgact aatttcaaag gctttttctt cttcgtcaaa
     6121 tccatagtaa gcttcttctt ccatatcgga ctcgtcgtag agcaaaaatt ctggtgtctt
     6181 gtctaacaat tcagctgtag catctaaaag tgttgccaga ccttgcttgg tcaatccaga
     6241 aattgggaag atagctggta actcttcaaa ttcatcataa ttttcagcca attttttctt
     6301 aaagtcttca agattttcct gactctcagg catgtccatc ttattagcta caataatctg
     6361 tggacgctcc atgaggcgaa gattgtaaga ctccagctct ttattgatag ctaggtagtc
     6421 ctcatatgga tcacggccct cgctagctga catatcaatg atgtgaagga taacacgtgt
     6481 acgctcgatg tgacggagga actgagttcc caaaccaaca ccttgactag ccccttcaat
     6541 caaacctggc aagtcggcta ctgcaaagga ttcacctgat tgggtgcgaa ccatacctaa
     6601 atttggtaca atagtggtaa agtggtaggc accaatttta ggcttagctg aggtaataac
     6661 acttaaaagt gttgacttcc ctacagatgg gaatcctact aaaccgacat ctgccaagat
     6721 ttttagttcc aattgtaact cacgttcctg acctggttct ccattttcag agatttccgg
     6781 tgcaggattt tttggtgtcg cgaaacgaat atttccacgt ccaccacgac caccgtgggc
     6841 aacgataaat tcttgcccat gttcaatcaa atctgttaaa accttgccag tctccgcatc
     6901 acgaacagtc gtaccttgtg gtactcgaac tctaaggtcc tcagcaccac gaccatgcat
     6961 ccctttggtc atcccttttt caccagaatc agccttgaaa tgacgattgt agcggaaatc
     7021 catcaaggta cgtagtcctt cgtctacaac gaagaccaca ttgcctccac gaccaccatc
     7081 accaccccaa gggcctccat tagggacata tttttcacga cgaaaggcaa ccataccatc
     7141 gccaccatta ccagccttga ccttaatctt agctgtatct aaaaacatac tcattatttc
     7201 ttctcacttt aaaaaagggc tgggaaatcc cagtcactaa attttcttga atctatttta
     7261 tagattactg agggcaccaa ttgcagttgc aaaaattccc aataaacttg ctactagcat
     7321 gataatcacg ataaacaagg ttattttctc aaacatagtt tttttacgat ttccattatc
     7381 tccaaatgcc atttttctct ccttcgttac attctatttt ctattatctt agcatgaatt
     7441 gagctagttt tcaatagtca gttgctactg gtgcgataat ccataaaata atgtaagcta
     7501 caattccagc tccgtaacag caagtaagaa caccccaaat gacttgaacg atagtcggat
     7561 ccatatccag ataatgggcc accccagcac aaacacccgc aatcttttta tcacgaccgc
     7621 ttctcattaa ttgttttttt catagctgtt cctctttcta ttcttcatgg tctttccaat
     7681 agtctctcat actgatcaac tgtgtttttg aagccttcaa catgcgtcta gatcctttta
     7741 caggtacagc aaccacctgt tgctgaagat acaaaactgt cttaaagata cgctgactga
     7801 ccgtatcatc tttagccaaa tctttctgga agttatttga aataatggca tccagataaa
     7861 actcatgcac tcgtttccca aagttctcag ctgaaatctc atacaatttc tctgataagg
     7921 tatgctcgtt catgtctggt gttgcaatca gggcttccaa aatagcacca gccaaatcat
     7981 gttctccata gtacaaggtt ccaaacattt tatcactgat gaggttgttc aaataaggat
     8041 ttccgtgagc aatgacaggt gttccactgg ctaagctttc caagtaggtc aaaccttgcg
     8101 tttcgcttgt cgatgccgaa atgaagaaat ccgccgcttt atagtaaaga gccgtctcac
     8161 taggagcaat catccctgta aagatgactg agtcttgaat ctctaggttc tgggcttgct
     8221 ctttgaggtc attcagataa gggccatccc cagctactac cagtttaacc ttgtcttcct
     8281 ctttcagaac atcagcaaag gctgctaaaa ctgcttgaat atttttttca taggagattc
     8341 tcgaaagact aagcaacgtc ttttcaccat cttgaatccc tagtttacta cgcagttctt
     8401 tcaaattttc ctgcttgatt tccggacgct caaacttggc taattcaatc ccagtaggaa
     8461 tgacccgttt ttcaaccttg accttataat cagatagcaa gtcacggaca atctcactag
     8521 ggcaaataac cccatccaca tcatgcagga aacctctaac cagatacttg accatactcg
     8581 accggatcaa catcccctta gcaatataat ggacatagtc ttcatactgg gtgtgatagg
     8641 tatggatgac tggaattttc aattcacgcg caatccaaat ccccaacagg ccaagagaaa
     8701 attctgtctg agtatggata atatctagct gatactgttt agcaatttca agtgccttgc
     8761 taaaacctcg gtaggcaaag cgacgatcct taaaagcaaa gaaaggaaca cttggaatgc
     8821 ggataatttg ccaatcttcg tagcgattga catccttatc tgtcgtcgta aagataaaaa
     8881 cagcatgtcc ctgcttttca agttctgttt tcaaggttcg aatactggtc gcaacaccag
     8941 aaacctgagg aaaataggta tctgtaaata aaccaattcg catagattac ctcacttttt
     9001 actttctccc taaagcggct tgtttctcat aaaagtccaa ccagatttgt aacagatgct
     9061 cttcagaata ctctctggaa atattcttgg ccttttcttt gagatctttt aagacagcag
     9121 gatttgcttg atattccaaa atagcctctt tcatctcttc tctacccgct gtcgcccgat
     9181 aatttccctc caaaatcacc ttatagagat ctaaatcacg caacataata ggagcctcac
     9241 aactcgcagc ttctaaaata gtcataggaa agagctcatt gtaactaggc aacaagaaaa
     9301 gatccgctag agcatacaat tcgcgcatcc gctctggcga tacaatgcct ggaaaaatca
     9361 aatttttagg gggattttcc ataattttct tatagtgttc ataaccatct gtcataccac
     9421 caaaagagaa gccaccagcc cagataaagg taatctgagg caattcctca gccagacgga
     9481 taaagtcatc aatcccttta cgtttctgaa cttgcccagc acctactacg ataaactgat
     9541 tgtcactaag accaagatct gtgcgcagtc tgactacctc ttcttgtggt agaggatgcc
     9601 atttttcctt gttgacaaag ttaggaatat aggtcacttt ttcacgtgga ataccagctg
     9661 ctaccaaatc ctcaataaac ataggattga ccacaaccaa gtgctccatc cggttgtaaa
     9721 aagaaaatac atagcgtttc acaattccct ttaagaaaaa tggaattttc aaacttcctt
     9781 caagtgtagc tggcaagaaa tgcacatagc caatctttct ccctgagcgt ttcttttgga
     9841 aggttgataa ataataggga aaatcaatcg tatgaaagtg agtcacatct gcctcgattg
     9901 gaagattttc tgtaacaatc aattggtcct tggcagcacg gtgaagaaga cgaactaatt
     9961 cacggtaagc acctgaaact ccctgtcctg ctactttctc acttgaactc aacatattga
    10021 tgcgtaattt ctttttctcc ataactacta ttatatcatt ttctttgaat aaaatcagca
    10081 aaagaaagga gagactagag gaaaagaggg gaaatttcct cgcttttcca acagtctctc
    10141 acatgctttt atatgtttac ttaatgccta gggcaatgcg ggcatagcga ctcatttttt
    10201 caactgtcca ggctggatac cagactaatt taacctcagt atccgttact tctggaacct
    10261 ctatcatagc atcataaatc tggtctgtca aaaggtctgc taagggacaa cccatagttg
    10321 tcaaagtcat gtcaatctct gtttgccctg tgtcaccgtc aaaacgaatc tcatagatca
    10381 aaccaagatt gacaatatcg attcccaact cagggtcgat gacttcttcc aaggctgtta
    10441 aaatccgtgt tttgatgttt tcaatttgct cttctgtata agccatattt tcctcactct
    10501 tagtcttcaa taaaatcacg aagcggtttg ctgcggcttg gttggcgtag ttttctcaaa
    10561 gcctttgctt caatctgacg gatacgctca cgagttacgt taaagacttt ccccacatct
    10621 tcaagtgtgc gcatttttcc atcatctagt ccaaaacgta gacgcagaac attttcttca
    10681 cggtctgtaa gagtatctaa gatttcatcc aattgctcac gcaagacgat acgagtcgta
    10741 taatccactg gattttcaat tacttcatct tcgataaagt ctccaaggtg gctatcgtcc
    10801 tcttcaccga taggagtttc aagagatact ggttcttggg caatcttcaa gatttcacga
    10861 accttatcag gtgtcatatc cattcgttca gcaatctgtt ctggtgtcgg atcttgcccc
    10921 aattcttgaa ggagattccg ctgttcacga accaatttat tgatagtttc aaccatgtga
    10981 actgggatac ggatggtacg agcttgatcc gcaatagcac gagtgatagc ctgacgaatc
    11041 caccaagttg cataagttga aaacttgaac cctttagaat agtcaaactt gtcaaccgcc
    11101 ttcatcaagc ccatatttcc ttcttgaatc aagtcaagga actgcatgcc acgaccgaca
    11161 tagcgtttgg caatagaaac aaccaaacga agattggctt ccgcaagacg ttgtttggct
    11221 tcgatatcgc cagcttcaac agccagtgcc aactctttct cctcttcatt ggtcaagaga
    11281 ggaacgaccc ctatttcttt caagtacata cggacagggt cattgacctt agcagaagtt
    11341 gacccaatca agtcctcatc gctgagttct ggttcttctt cattgctaag aacacgcgca
    11401 cttggatttc cttcgttatc tgtgatagaa atgcctgcat cctgaatccg ttgcaagaga
    11461 tcttcaatcc catcagcgtc caaggtaaaa ggaataacca gacttgcatt gatttcatca
    11521 tctgttgctg tccctttttg cttatgatta cggataaatt ctgctacctg tacgtcaaat
    11581 gttgttactt ctttttgttt tgttgccatt attactccat tcttctcttt tgggaaatta
    11641 aacgttccaa ttcttctagg gctgtatctg tatctcctac atggctagct tcctgcacct
    11701 tctttttgat tctcatattg tcctgattca agagagcctt gtttcgagtc atctctactt
    11761 cactaagttc ctgcggcgat atctcagcag gcaaatcctg agctaaaact tggtaccaag
    11821 ctctttcaac ttcctctgtc tgctctgcta aaacttctgg aggaagattt ccatactggc
    11881 caagcaagtc atataagacc tgaaattcag gtgtagcaaa tgcaaagtct tctcgcaaac
    11941 ggtaatcgtt caaaacaaga ggggattcca tcatccgata gagtagatgg gcttctgccc
    12001 tcataatagc cgataactgc ttggtgatag gcatggtgat tggcgtcggt ctggaaattc
    12061 cttccatgcg attctgcctt tgcacctgac gactctcatt aacaatctgc tcaatctggg
    12121 tataatcaaa ggacgccaga ctgtcagcta aaatatgaat atagctgttt tgagcagcga
    12181 tggacttttc ttgaacaatc aagggagcta ttttttcaat aaactcaatc tgagcctgca
    12241 gattttcact gttttcaggt ttgtactgat gaatgtagaa ctcaatcgga ctaatacgag
    12301 tttttgttaa tagataggcc aagtcttctg gaccattttt ttgtagatac tcatcaggat
    12361 ccaagttatc aggcatgcta actatttgca caggcatatc accaatttca tccaatgctt
    12421 tcaatatcgc ggcttgccca gccttatctc catcgtaaac aagaaccaat ttcttggtta
    12481 accttttcag atgctcaaca tgctctcgac tcaaggccgt tcccatcgac gccacagcat
    12541 tttcgattcc agcccgatag gctgcaataa catccatgaa tccttccatc aggtaaatct
    12601 cactagcttt tccagaagat ctttttgccc tatccatatg atataattcg taacttttgt
    12661 taaaaattgc agtcgatcgg ctgtttttat acttagaagt ttgtgaatcc gttttttgcc
    12721 agatacgacc tgagaaggca atgacctttc cttggtcatt tgtcagggga aacataatgc
    12781 gattgtgaaa ggtgtctaca aattgattgg catccgagag ataaaacagg cctgaatcca
    12841 gtaaatcctc ttcacgatac tgatcagaca aacgttgata gagatagttt cgttctggag
    12901 gtgctaaacc aatccaaaaa tgtttaagca cttcatctgt caaaccccgc tgataaaggt
    12961 aatttctggc ctcttctccc atagtcgttg tcatgagaat agcatggtaa aatttggctg
    13021 catcttcgtg catatcataa agagcttggt gaggcgaggc tggcttctgt tcactataaa
    13081 gcggtttttc aacctcaatc ccgacacgct gacctaagat ttggacagcc tccataaagg
    13141 taaccccttg gtactcctcg atgaatttaa agacatcacc tgagcgacca caaccaaaac
    13201 agtggtaaaa ctgcttgtcc tctacaacgt tgaaagaagg tgttttttca ccatgaaaag
    13261 gacagagccc tagatagttc cgtcctgcct tttgtaaaga aatcacatct cctatgactt
    13321 ccacaatgtt ggcattgttt ttgatttctt caatgacttg tttgtcaacc atacacaata
    13381 cctccatgtt atcatagttt actttatata gtatacttta tttcagaaaa aaagtaaacc
    13441 atttcactca ttttccctac tttattcaaa gagttgataa taatcagaga ttttcatttt
    13501 tgctttttct tcttggttta aatcttggat aatccgtcct tctttcatga caatcaagcg
    13561 attgccgtat ttgagagcat cttccatatg atgagtaatc ataagggctg ttagctgatc
    13621 tttcttaaca aattcatctg tcaattccat caaagcaaca ctagtctttg gatccagggc
    13681 agcagtatgc tcatctaaca ggagtaattc aggtcgcttc aaggttgcca tcaagagact
    13741 caaagcctgt ctttgtccac ctgataagaa ctcaatcggt gtattcaagt gtttctcaag
    13801 accatttcct actttttcaa tggttgcctg aaattcatcc ttatagctag tcaagcgtct
    13861 tggtaacaat ccacgctttt caccacgaaa cttggcgatt aaaagatttt cagcgaccgt
    13921 catacgggga gctgtcccca tctttggatc ttggaagaca cgagacaggt acttggcacg
    13981 cttctcgggt gaaaacttag tgagatcttc acctaaaata cggatagttc cactagttag
    14041 tgataaggtc cctgctatag tgttaaagag agttgatttt ccagcaccat ttccgcccaa
    14101 aatcgtgata aagtcccgtt caaaaatttc taaggaaaca tcatttaaaa taatcttttc
    14161 ttcatcaaag ccatttttaa cgattttggt tgcatttttt aattctacaa ttgctgtcat
    14221 ttgcttaact tggctccttt caagattgtt tgcttaaatg ttggaatcat gaggcagact
    14281 gctaaaatca aagcactgta taaacgaagg taacttgtat taaagccaag tgcgataact
    14341 gcccacacta aaaattgata agcgatagaa cctacaacga tagtaaccaa acgctctgcc
    14401 aagctcaaac tcttgaaaat aacttctcca ataatcaaac ttgcaagccc cacaacgata
    14461 accccgatcc ctcgagacac atcggcatag ccttcttgct gagcaatgag ggcacctgca
    14521 agggcaatca caccatttga taagaccaag cccatgagct ccatgcgtcc agtatgaatc
    14581 ccgaaacttc tagccatatc aggattatcc cctgtagcaa tataggcttg tccgagttta
    14641 gtgtccaaga aaaagagcat gagagcaata acaatgctca caaagatgag acctgtcaag
    14701 agttgattca aatccgaatc aaaaggcaaa acatcctgaa tttgcttggt tccaagcagg
    14761 cctaaatttg cacgtcccat aatcaagagc atgattgagt gacaagaagt catcaccaaa
    14821 atccctgaga gcaaggttgg gatcttccct tttgtataaa gaaggcctgc tgccattcca
    14881 gccaaacaac ctgctcctac agcaacaagt gtcgctaaaa atggattcac gcctttggtt
    14941 atcaaagtga cagcaacagc tcccccaaga gggaaggaac cttctgtcgt catatctgga
    15001 aagtttaaaa tcctaaatgt cataaagatt cccagaccta gaatagccca gacaaatcct
    15061 tgagaaataa tggaaacaat catattttat ttaatccttt ctatattcat ctttttaaaa
    15121 aatgggaaga gtctcctcct ccctacctta tttattcgat gacttgtcct gcttctttga
    15181 gaacagactc aggaatagta atacctagtt cttgtgctat ttttttattg atgactgact
    15241 taccagttga aaagacattg actggggtat cggctggttt tgcacctttc aagacttgca
    15301 caatcatttt acctgttgcc acaccaaggt catgttggtc aattacaact gatgccaaac
    15361 cacctacttc taccatagct gtcgcactgg gataaattgg tttcttagaa ctttgattgc
    15421 tagagacaac cgttggaaat cctgatgcaa tggtgttatc aattggaacc caaatagcat
    15481 ctaccttgct agtcataaca gtgacagttg aggcaatttc atttgttgaa ggaactgcaa
    15541 atgtttccac tgtcagacct gctttttcag cataagcctt aaattcttcg acctgtgttt
    15601 ttgaattgtc ttcgctactt gagtaaagag ctccgattgt tttcacattc ggtgtcagag
    15661 ccttgatgag ttcaacttgt tgttgagctg gattgtggtc agatacccct gtaacgttgc
    15721 cacctggttt tttcaaatct ttaaccaagt tagcaccaat tgggtctgta atagcggcca
    15781 tgataaccgg taggtctttt gttgcactag ccaacccttg ggctgctggt gttgcgatac
    15841 caaccacaag gtcattccca tttgcaacca attgtttact cattgtcgca accttacttt
    15901 ggtcaccttc tgagttcata aaatcaattt taacttgatc atctttatat ccttcttctg
    15961 caagtccatc ttggatccct ttataaatca aatcaaggga tggatggctc acaaattgaa
    16021 gcacaccaat cttggttatt ttcttctcat ccttattatt ctgaacttct gatttattca
    16081 ttgaagaata aatcaagctt cctgctacta agactgctaa tgcagcaata attccaatta
    16141 aacgtttatt tttcattctg tttctccttt ttatttcctt taacatattt cacttatcta
    16201 aacaagcgac gccattgttt tctttccata ctaacctcca tcaaaaaaat cctcacacaa
    16261 aaaacttgtg tgaggacgtc gatgcgcggt accacctcaa ttatagggaa tttccctatc
    16321 gctcagtctc gcaataacga gatgcactgt aaggtgtgct taccgaattt ttataatttc
    16381 aaattctaaa tagcattcag cccaattttc atcattgcca cttatctgtt ttcagctacc
    16441 acagactctc taaaaagttt gtatgattac ttttctgaat ggttaaatta tatcattttt
    16501 aaactacttg tcaatagtct attagaattt ttttgatatt cacttttagc attcactctt
    16561 gaaaattatc aacctgaatt acgcaatcct gtcgcaattc cgttgatggt gatatgaatc
    16621 aatcgttctt gatcactgga caattctcca cgacgttggc gtttaatcaa ctccaactga
    16681 atatagttga gaatattaaa gtaaggcata cggtaatcca gactagcttt tagatatgga
    16741 ttgtcagcta agagttcgtc atgtccttca atagccaaga taacgttctt agtaacttgc
    16801 cattcattta aaatagtctc atagatggcc ttaacttgct cgtcttcaca aagtttagca
    16861 tattcaaaag caatattcat atttgatttt gacaaaacca tatcaacatt tgaaagaagc
    16921 gattggaaga aaggccaatt ttggtacata tctcgtaaga tagcaatatt ctctggattt
    16981 ttattgataa attccttgaa gcttgaacca accccgtacc atccagggaa cataacacga
    17041 ctctgtgacc atgagaatac ccaagggatg gcacgcaatc caccgatttc agtaatagtc
    17101 ttacgagcgg ctggacgaga accaatatta aaacttgaaa tagccttgat tggacttgac
    17161 tcgaagaaat aatcatagaa atgctcatta ccaaagacca aatcacggta gatatcgtaa
    17221 ctacggtcca ctacttgatc cataatggct tcataacgat ttggggtatt ggtatcgctc
    17281 ttcttctgag taatcatacg gttaatagct gccgatacta gcatttcaag gttatagtaa
    17341 gcggcgtctt tgttaccgta tttattccca attacttcac cctgctccgt caagcggata
    17401 cgatccttga tagacttgag cggttgagat gtaatggctt cataggttgg cccaccacca
    17461 cgaccgacag taccaccacg accatggaag aaggtaacct taacgccaaa ttcatctcca
    17521 atagcagtca attgttgttg agccttgtag agggtccaac atgatgacaa gtaaccgcca
    17581 tctttattac tgtcagagta gccaagcatg atttcttggt agttatttcg tgagtcaatc
    17641 cattttttgg caaggctaag agaaagatat tttctcattg tttcctctga atgatccaag
    17701 tcttcaattg tttcaaaaag gggaacaatc tgaacacgcg ccctttccgt atccaccagt
    17761 cctacttctt ttaacagaat agctaattct agcatatcag aaagactggt tgcatgtgaa
    17821 atgatggtct gacggatgac atcatctccc aacttatctt tcaaaacacg agccgtctta
    17881 aaaatagcta attcttttgc taataattct gatttttctg cgtgagtcgc agaaagaatt
    17941 cggggatctt cttctaattc tttcaagaga aggtcacact tttcttcttc gctcaactcg
    18001 ctataacgag aatgaattcc tgctgatttc aagagttctg ccacacaggc ttcatagacg
    18061 ctagagtctt gtcgcatatc aattgacgct aagtaaaaac caaatatctc tactgcctgc
    18121 aataattcca caaaatcacc tgaaatcaag gactcaccct tattttctag tagagaatct
    18181 cgaatggcaa tcaaatcctt gtagaaatca ttggcggttt cataacgagt cccaacttct
    18241 tcatcctcaa tcagataggt tttagttgcc tgaatttttg attgaatatc aaacaaggca
    18301 cgacggtaaa gctctttttc gcggtaaata gagttatcct tggattgacg agccatttct
    18361 ctgacttgct tgctgacgtt gacaatgcta gttgaaagag aaaattcacg ataaagttgg
    18421 taaatctttt tatcatagta gttcatgatg acttcacact gagtgagtgc agactgcttc
    18481 aaggtctttg ctgtaacaaa tggatttcca tcacggtcac cacctatcca catacccatg
    18541 gtgattggtt tagcctgttt taaattcaga ccatgcgctt gcgctaagcg cttatactcc
    18601 gtcgtcaaat gagggacagc tttcaaaaag gagctgttgt aatattccat agcattcgtg
    18661 atttcgttag tcacttttaa ttttttctca cgaatcatgt ctgtctgcat gataatttcg
    18721 atgtaacgac gcaaatcatt gtgccattta tctttattga ttaaccccaa cttaacatca
    18781 cggtatttac gcaaaagact atgaatatga tttgttaaat ccaacatact tttgcgttgc
    18841 acttgtgttg gatgggctgt caaaacaggg acaacattca agtgttctag gatctcaacg
    18901 gcattttcct tttctgctac caatttaatc gttgtagata atttacctaa atagtcctga
    18961 tcaatattat tttgatgatt gatttcataa gctaaatcca catcctctga aatattaatc
    19021 aaaagaggta agatagagaa atagcgtgaa atatagacca tttcatcatt tgataagcta
    19081 gtcactagac ggtttagacc ttgataatct tcctgcgttg ataattcttt caactgtatt
    19141 attttttcaa aggtctctgg ggcaagcata tttttagtaa tatcttccag taattctgtt
    19201 agaatcaaga cttcttcttt cacaacactt ttattactat aattttctaa tttttgaaga
    19261 gacataaact atcctttctt tatcctactt caacagaagg ttcattggtt gattttccaa
    19321 ttctcggtac aacttagcgc gtttttcact ggcatcaata tttaagacaa aggctactgc
    19381 cactgataag actagaagac tatttccacc ctgggataag aaggggaaag tcactcctgt
    19441 agatggaatc aagcccgaaa tccctccgat attgacaaat acctgaacca acatcatccc
    19501 tccgacaccg agtgcaacca tggcattgaa aggattctcc gctcggatac cgaccaagat
    19561 aatccgcaaa atcatgaaaa acaagagagc taaaataaga ctggcaccaa caaagccaaa
    19621 ttcttcaatc acgatagaaa agacaaagtc tgtatgagct tctggcaaat aacctcgttt
    19681 ttcaatcgag tttccaagac ctagaccaaa ccaaccgcca ttgaccatgg caaaataaga
    19741 attagctaac tggtgacctg catcagcacg atcggcaaaa ggattaaaaa aggcactaaa
    19801 gcgcttggct acatagccaa atactggaat ttttgaaaag gtctcaacac cgattaggct
    19861 gatagtggtc aagacaaaga cagaagtggc agatacgagc gccagaatgg ttgaaaacca
    19921 gcgataagcg attccactaa ctgtatacat aatcaaggaa accaagacta aaatagtcgc
    19981 atttcctaaa tcagggaaaa ttcccaaact tccaatcaga actaggagaa cgaatcgcca
    20041 atcattaaaa gcacggggaa gccattgatt ttgagtcaaa acttgaaaat cataagtagc
    20101 tatttcttct tgctgtttgg agaatcggtg agctaaatac caaataataa tgatttttaa
    20161 gtactcagct ggctgaatag ttactcctgc aaccgaaatc caaccgtatg ccccgtttac
    20221 ggaaatacca ataaaacgag ccaagaacaa taaaagcatt tctattaata taactaaaat
    20281 gattagtcgc tcatttctca aaaaatctag tctcaattta taaattaagg caatcagtat
    20341 caaactaaca atccaaaaga ttccttggtt tcgaaccaac tgcaaggcac tcttgccttc
    20401 ttcaattaaa atagcactgg tggtcgaata gaccacaatc aagcccaaaa tagataaaag
    20461 caagtaggga atcaagatgg aataatttaa taagtgcctc ttactaatct tcatagcatc
    20521 accactctac taggatacag ataaatctgt tccaaattcc gttttatttt ctagttttga
    20581 ttgctattat accatttttt cagaaagaaa aaaactctta ccttacaatc cttcgaattt
    20641 tactgttttt acagaattaa ggcagaattt gaaatcgtaa aaccattttg aataagagtt
    20701 gcttcaaccc attgactccg acggattaag ttgctttcgt gaatgccaaa atcagccgca
    20761 atttgttcat aagtgctcta ttctcgcatg tattgaatag ttaccataag gagatcgtct
    20821 aggcttaatt tgctttttcg tccacctttt gcgcgtttac gttgataagc tgtttttaac
    20881 acagctaaca tctcttcaaa agtcgtgcgc tgaacaccta caaggatctt gaaacggcta
    20941 tcgcttaatt gtttacttgc ttcatcatcc atagttttat tgtattgtat ttttgtttcg
    21001 caggaagtct attattcctt tcgttccaag gtttttacgc tttttcaagc aatcctacac
    21061 actccacgtg atgcgtctgt ggaactcaaa aggttcgggg aaccttttga ggattaattg
    21121 cgtttctcta acaaactcac acattcaacg tggcatgact gatgaagtca tcataagaat
    21181 ttcacttgct gctctttctc ggtggcagcc tgaacggtca gaagtatcat tgtatatcat
    21241 ttgtatcatg gcatataaag tatcgctcta ttttattgat ggttttaata tcgctcttat
    21301 catggcgaac cattcaaatc tgctccacaa atgaggaaag taccattttg gactattcaa
    21361 ttgtcaaaga acaaataaaa aaaatcttcg cattttcaca ctctatgaaa ttgaggagat
    21421 tattttctaa agcgaatttt atcaggaaaa tccgtcaatc ccaataggta gtctgtactg
    21481 acatcgtaaa agtttgagag agttaccaat atatctgctg ttaacgtatg ctcaccccgt
    21541 tcaattttag cataagctga gtctgtaaac gaaagtattt tagctatttg tttttgggtc
    21601 aaatcgtgat cttccctcaa atctctcaaa cgtctgtaca tctagctttc tcctgagaaa
    21661 agt
//
LOCUS       DAVKWS010000032        21595 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKWS010000032 DAVKWS010000000
VERSION     DAVKWS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.032"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..233
                     /locus_tag="VW852_001310"
     CDS             <1..233
                     /locus_tag="VW852_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0272451.1"
                     /translation="HIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTY
                     YWTFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(430..1350)
                     /gene="cysK"
                     /locus_tag="VW852_001311"
     CDS             complement(430..1350)
                     /gene="cysK"
                     /locus_tag="VW852_001311"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV0272452.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1635..2270)
                     /locus_tag="VW852_001312"
     CDS             complement(1635..2270)
                     /locus_tag="VW852_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV0272453.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPILGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2326..3624
                     /locus_tag="VW852_001313"
     CDS             2326..3624
                     /locus_tag="VW852_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0272454.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3621..4283
                     /locus_tag="VW852_001314"
     CDS             3621..4283
                     /locus_tag="VW852_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV0272455.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNNDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4363..4911
                     /gene="raiA"
                     /locus_tag="VW852_001315"
     CDS             4363..4911
                     /gene="raiA"
                     /locus_tag="VW852_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV0272456.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            5047..7020
                     /locus_tag="VW852_001316"
     CDS             5047..7020
                     /locus_tag="VW852_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV0272457.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNIQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            7017..7469
                     /gene="rplI"
                     /locus_tag="VW852_001317"
     CDS             7017..7469
                     /gene="rplI"
                     /locus_tag="VW852_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV0272458.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7512..8864
                     /gene="dnaB"
                     /locus_tag="VW852_001318"
     CDS             7512..8864
                     /gene="dnaB"
                     /locus_tag="VW852_001318"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV0272459.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8866..9132
                     /locus_tag="VW852_001319"
     CDS             8866..9132
                     /locus_tag="VW852_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272460.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9221..10567
                     /gene="cbpD"
                     /locus_tag="VW852_001320"
     CDS             9221..10567
                     /gene="cbpD"
                     /locus_tag="VW852_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV0272461.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDDYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10650..10752
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10822..11112
                     /locus_tag="VW852_001321"
     CDS             10822..11112
                     /locus_tag="VW852_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV0272462.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11075..11833
                     /locus_tag="VW852_001322"
     CDS             11075..11833
                     /locus_tag="VW852_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272463.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWFNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11871..12878
                     /locus_tag="VW852_001323"
     CDS             11871..12878
                     /locus_tag="VW852_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0272464.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12878..13606
                     /locus_tag="VW852_001324"
     CDS             12878..13606
                     /locus_tag="VW852_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272465.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13725..14183
                     /locus_tag="VW852_001325"
     CDS             13725..14183
                     /locus_tag="VW852_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV0272466.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQKLMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            14185..16617
                     /locus_tag="VW852_001326"
     CDS             14185..16617
                     /locus_tag="VW852_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0272467.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVSAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17259..17912
                     /gene="rr06"
                     /locus_tag="VW852_001327"
     CDS             17259..17912
                     /gene="rr06"
                     /locus_tag="VW852_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV0272468.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17909..19249
                     /gene="hk06"
                     /locus_tag="VW852_001328"
     CDS             17909..19249
                     /gene="hk06"
                     /locus_tag="VW852_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV0272469.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            19344..19871
                     /locus_tag="VW852_001329"
     CDS             19344..19871
                     /locus_tag="VW852_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV0272470.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGRMKG"
     gene            19951..>21595
                     /gene="pspC"
                     /locus_tag="VW852_001330"
     CDS             19951..>21595
                     /gene="pspC"
                     /locus_tag="VW852_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEV0272471.1"
                     /translation="MFKSNYERKMCYSIRKFSIGVASVAVASLVMGSVVHATENEGTT
                     QAPTSSNRGNESQAEQRRELDLERDKVKKEVREYKEKKVKELYSKSTKSRHKKTVDIV
                     NKLQNINNEYLNKIIQSTSTYEELQKLMMESQSEVDKAVSEFEKDLSSSSSSGSSTEP
                     EASDTAKPNKPTELEKKVAEAQQKVEEAEKKAKDQKEEDYRNYPTITYKTLELEIAEF
                     DVKVKEAELELLKVKAKESRDEKKIKQAEAEVESKQAEATRLKKIKTDRKKAEEEAKL
                     KEAVEKNAATSEQGKPKRRVKRGALGEQATPDKKDYFEKDFRPAFNKNQQMVAIQESL
                     NKLDDETKTVPDGAKLTGEAGNAYNEVRDYAIKVVSENKKLLSQTAVTMDELAMQLTK
                     LNDAMSKLKEAKAKLVPEVKPQPENPEHQKPTTPAPDTKPIPQPEGKKPSVPDINQEK
                     EKAKLAVATYMSKILDDIQKHHLQKEKHRQIVALIKELDEFKKQALSEIDNVNTKVEI
                     ENTVHKIFADMDAVVTKFKKGLTQDTPKEPDNKKPSAPKP"
BASE COUNT         6825 a         3563 c         4865 g         6342 t
ORIGIN      
        1 ggcatatcaa gacgagtcaa tactatttgg agccccttta taacctcttg cgagagagac
       61 tattgactca gcccttactt catgcggatg aaacttctta tagggtgcta gagagtgata
      121 gtcagctgac ttactattgg acttttttgt ctgggaaagc tgagaaacaa gggattacgc
      181 tttaccatca tgtcctaatc gatttgttca tatcttattt caatccatta taaatagtga
      241 gaaatatcta tcctatcttc tagaatgtct tccaaacgtg gaaactctcg taaacaaaga
      301 ggttttagag gcctatttac catggactaa agttgtacaa gaaaagtgca aataagaaat
      361 ctccagatta ggaactatcc gtgagttctc tagtctggag atttttcaat agacttcgtt
      421 attggacggt tacaattcat aaagtgctgt agagagataa cgttcaccgt tatctggtgc
      481 tagggcaagg acttttttac ctgtacctaa ttttttggca acctcgatgg ctccgtagat
      541 agctgcagct gaggaaatcc ctacaaggaa gccttctttt ccaccaattt cacgtccgag
      601 tgcaagagcg tcatctgatg ttacacgaac gataccatca taggctttag tatcaagtgt
      661 atcaggaata aatccagctg agataccttg aattttgtga ggaccaggtt tttcaccaga
      721 tagaatagca gattcatctg cttctactgc aaaaacttga atgttagaat tttctgattt
      781 gagtgcatga gaaacaccag aaatcgttcc accagtacct actccagcaa caaaggcatc
      841 taatccatct ttaccgaaag cagctagtat ctcagctcct gttgttcttt cgtgtacttc
      901 tggattagct ggattgtcaa attgaagagg aaggaaacca tcacgttcag cagcgatttc
      961 ttgagcctta gcaatagcac ctttcattcc ctcgctacca ggagttagga cgagttcagc
     1021 accataagct tggataattt tacgtcgttc tacactcata gtttcaggca taacgatgac
     1081 gactttatac cctttagcag cacctaccca tgaaagtcca ataccggtgt ttccacttgt
     1141 tgcttcaaca atagtagaac caagtttcag aataccatct tgttcagctt tttcaatcat
     1201 gctaagggca atacggtctt ttacagatga accaggatta aatgcttcaa gctttatata
     1261 gacgtctgca gcaccttctg gcacgatgtt gttaagttta acaatcggtg tttgaccgat
     1321 taattcagta atgttgttat aaatagacat atgaatacct ctttgtataa gatatcttta
     1381 gtataacgct atccatacca tttgtaaaat atagaaatcc tatcgaagcg ataggatttc
     1441 tatatatcat agatcaaagt cattaatttt caagtgattg cgttcagtta attctagtaa
     1501 ataggtgtga aggaaaacga tatcaggttt cgtttgaatt gcttcatttt ctagtatatt
     1561 ttggtacgca tctttttatt ttgctcgcta aagtagttgt gagaatgtca acgaaacgct
     1621 gtattattca ttgtttacac taagtttaca ggaacttcaa cctctcgtaa accttggtca
     1681 gttaaagtga cttttccatt aaaaaactcc acaagtgcag ctttaatagt ttctttttct
     1741 tctttatcaa cataaatcat cgtatcgact tgatctgtaa agtttgtatc cagctccatg
     1801 agatcatgtt ctttaaggaa gttactgtac tcttggtact gagcataaga catttgaata
     1861 gcaatgccag cctgttcttt tatttcaata ataccaattt ctttgacagc taaggcgaca
     1921 ctgccggcgt aagcacgaat tagtcctcca gcgcctagtt taataccacc aaagtagcgt
     1981 gtcacgacca cacagacatt ggtgagattg tgattttcta gtaccccaag tatgggaaca
     2041 ccagcagtac cactaggctc accatcatca cttgtacgtt taatttcact acgttctcca
     2101 ataataaagg cagagcagtt atgtgtcgct ttgtagtgtt cttttttgat ggcagtaatg
     2161 aagtcacgag cctcttcttc gctataaaca cgcttggcat ggcagataaa gcgagatttt
     2221 ttgatttctt cttggacttg gccgtcctct ttaattgtcc taaattccat gatttaattg
     2281 tactaaaaaa tcatctaaag tgctagtttt tacgaataaa gaagtatgaa agtaaattta
     2341 gattatctcg gtcgtttatt tactgagaat gaattaacag aagaagaacg tcagttggcg
     2401 gagaaacttc cagcaatgag aaaggagaag gggaaacttt tctgtcaacg ttgtaatagc
     2461 actattctag cagaatggta tttgcccatc ggtgcttact attgtcgaga gtgcttgctg
     2521 atgaagcgag tcagaagtga tcaaacttta tactattttc cgcaggagga ttttccgaag
     2581 caagatgttc tcaaatggcg cggccaatta actccttttc aagagaaggt gtcagaggga
     2641 ctgcttcaag cagtagacaa gcaaaagcca accttagttc atgcggtaac aggagctgga
     2701 aagacagaaa tgatttatca agtagtggct aaagtgatca atgcgggtgg tgcagtgtgt
     2761 ttggctagtc ctcgcataga tgtttgtttg gagctgtaca agcgcctgca acaggatttt
     2821 tcttgcggga tagctttgct acatggagaa tcggaacctt attttcgaac accactagtt
     2881 gttgcaacaa cccatcagtt attgaagttt tatcaagctt ttgatttgct gatagtggat
     2941 gaagtagatg cttttcctta tgttgataat cccacgcttt accatgctgt caagaatagt
     3001 gtaaaggaga atggattgag aatcttttta acagcgactt cgaccaatga gttagataaa
     3061 aaggtccgtt taggagaact aaaaagactg aatttaccga gacggtttca tggaaatccg
     3121 ttgattattc caaaaccaat ttggttatcg gattttaatc gctacttaga caagaatcgt
     3181 ttgtcaccaa agttaaagtc ctatattgag aagcagagaa agacagctta tccgttactc
     3241 atttttgctt cagaaattaa gaaaggggag cagttagaag aaatcttaca ggagcaattt
     3301 ccaaatgaga aaattggctt tgtatcttct gtaacagagg atcgattaga gcaagtacaa
     3361 gcttttcgag atggagaact gacaatactt atcagtacga caatcttgga gcgtggagtt
     3421 accttccctt gtgtggatgt tttcgtagta gaggccaatc atcgtttgtt taccaagtct
     3481 agtttgattc agattggtgg acgagttgga cgaagcatgg atagaccgac aggagatttg
     3541 cttttcttcc atgatgggtt aaatgcttca atcaagaagg cgattaagga aattcagatg
     3601 atgaataagg aggctggtct atgaagtgct tgttatgtgg gcagactatg aagactgttt
     3661 taacttttag tagtctctta cttctgagga ataatgactc ttgtctttgt tcagactgtg
     3721 attctacttt tgaaagaatt ggggaagaga actgtccaaa ttgtatgaaa acagagttgt
     3781 caacaaagtg tcaagattgt caactttggt gtaaagaagg agttgaagtc agtcatagag
     3841 cgatttttac ttacaatcaa gctatgaagg attttttcag tcggtataag tttgatggag
     3901 acttcctgtt aagaaaagtt ttcgcttcat ttttaagtga ggagttgaaa aagtacaaag
     3961 agtatcaatt tgttgtaatt cccctaagtc ctgatagata tgctaataga ggatttaatc
     4021 aggttgaggg cttggtagag gcagcaggct ttgagtatct ggatttatta gagaaaagag
     4081 aagagagagc cagttcttct aaaaatcgtt cagagcgctt ggggacagaa cttcctttct
     4141 ttattaaaag tggagtcact attcctaaaa aaatcctact tatagatgat atctatacta
     4201 caggagcaac tataaatcgt gttaagaaac tgttggaaga agctggtgct aaggatgtaa
     4261 aaacattttc ccttgtaaga tgaggaaaaa atttgcaaaa atagaatgaa agcgttataa
     4321 taaaatcata aaaacaaaaa tgtttagaaa gaaggtactc atatgattaa atatagtatc
     4381 cgtggtgaaa acctagaagt aacagaagca attcgtgatt atgtagtttc taaactcgaa
     4441 aagatcgaaa agtacttcca accagaacaa gagttggatg cccgaattaa cttaaaagtt
     4501 tatcgtgaaa aaacggctaa agtggaagta acgattccgc ttggatctat tactctccgc
     4561 gcagaagatg tatctcaaga tatgtatggt tcaattgacc ttgtaactga taaaattgaa
     4621 cgtcagattc gtaaaaataa aacaaaaatc gagcgtaaaa ataaaaataa ggtagcaact
     4681 ggtcaattat ttacagatgc tttggtggaa gattcaaata ttgtccagtc taaagttgtt
     4741 cgttcaaaac aaattgattt aaaaccaatg gatttggaag aagcaattct acaaatggat
     4801 ttattggggc atgatttctt tatctatgtg gatgttgaag atcagacaac caatgtgatt
     4861 tatcgtcgtg aggatggcga aattggtttg ttagaggtta aagaatctta atttcaatat
     4921 gtgtaaaggt aggtttactg aattgtaaac tacctttttc ttataattga tagaattact
     4981 gattacactt ttaaaaagtc gctttttgag gggtattatg gtataatggg tgccaagagg
     5041 ttttgaatga aaaaatttta tgtaagtcca atttttccta ttctagtagg attgattgcg
     5101 tttggagtct tatccacttt cattattttt gttaataata atctgttgac ggttttaatt
     5161 ttgtttcttt ttgtaggagg ctatgttttt ttatttaaga aactgagagt gcattataca
     5221 aggagtgatg tagaacagat acagtatgta aaccaccaag cggaagaaag tttgacagct
     5281 ctattggaac agatgcctgt aggtgttatg aaattgaatt tatcttctgg agaggttgag
     5341 tggtttaatc cctatgctga attgattttg accaaggaag atggtgattt tgatttagaa
     5401 gctgttcaaa cgattatcaa ggcttcagta ggaaatccgt ctacttatgc caagcttggt
     5461 gagaagcgtt atgctgttca tatggatgct tcttccggtg ttttgtattt tgtagatgta
     5521 tccagggaac aagccataac agatgaattg gtaacaagtc gaccagtgat tgggattgtc
     5581 tctgtggata attatgatga tttggaggat gcaacttctg agtcagatat tagtcaaatc
     5641 aatagttttg tagctaattt tatatcagag ttttcagaaa aatacatgat gttttctcgt
     5701 cgggtaagta tggatcgatt ttatctattt actgactaca cggtgcttga gggcttgatg
     5761 aatgataaat tttctgttat tgatgctttc agagaagagt cgaaacagag acagttgccc
     5821 ttgaccttaa gtatgggatt ttcttatggc gatggaaatc atgatgagat agggaaagtt
     5881 gctttgctca atttgaactt ggctgaagta cgtggtggcg accaggtggt tgttaaggaa
     5941 aacgacgaaa cgaaaaatcc agtttatttt ggtggtgggt ctgctgcttc aatcaagcgt
     6001 acacggactc gtacgcgcgc tatgatgaca gctatttcag ataagattcg gagtgtagat
     6061 caggtttttg tagtcggtca caaaaattta gacatggatg ctttgggctc tgctgtaggt
     6121 atgcagttgt tcgccagcaa tgtgattgaa aatagctatg ctctttatga tgaagaacaa
     6181 atgtctccag atattgaacg agctgtttca ttcatagaaa aagaaggagt tacgaagttg
     6241 ttgtctgtta aggatgcaat ggggatggtg accaatcgtt ctttgttgat tcttgtagac
     6301 cattcaaaga cagccttaac attatcaaaa gaattttatg atttatttac ccaaaccatt
     6361 gttattgacc accatagaag ggatcaggat tttccagata atgcagtcat tacttatatc
     6421 gaaagtggtg caagtagtgc cagtgagttg gtaacggaat tgattcagtt ccagaattct
     6481 aagaaaaatc gtttgagtcg tatgcaagca agtgttttga tggctggtat gatgttggat
     6541 actaaaaatt tcacctcgcg agtaactagt cggacatttg atgttgctag ctatctcaga
     6601 acgcgcggaa gtgatagtat tgctatccag gaaatcgctg cgacagattt tgaagaatat
     6661 cgtgaggtca atgaacttat tttacagggg cgtaaattag gttcagatgt actaatagca
     6721 gaggctaagg acacgaaatg ctatgataca gttgttatta gtaaggcagc agatgccatg
     6781 ttagccatgt caggtattga agcgagtttt gttcttgcga agaatataca aggatttatc
     6841 tctatctcag ctcgaagtcg tagtaaactg aatgtacaac ggattatgga agagttaggc
     6901 ggtggaggcc actttaattt ggcagcagct caaattaaag atgtaacctt gtcagaagca
     6961 ggtgaaaaac tgacagaaat tgtattaaat gaaataaagg aaaaggagaa agaagaatga
     7021 aagtaatctt tttagcagat gttaaaggaa aaggtaaaaa aggcgaaatt aaggaagtac
     7081 caacagggta tgcgcaaaat tttcttatca aaaagaatct agccaaagaa gcgactgctc
     7141 aagctgtagg tgaacttcgt ggtaaacaaa aatcggaaga aaaagctcac gctgagatga
     7201 ttgcagaagg aaaagcaatt aaagcacaac ttgaagcaga agaaactgtt gtagaatttg
     7261 ttgaaaaagt tggtccagat ggtcgtacct ttggttctat taccaataag aagattgcag
     7321 aagaattgca aaagcaattt ggaattaaga ttgataaacg tcatattcaa gtacaagctc
     7381 cgattcgagc ggttggtttg attgatgtgc cagtgaaaat ctatcaagat atcacaagtg
     7441 taatcaatct tcgtgtgaaa gaaggataag tttacacttt cttgacaaga ttgtaaaaga
     7501 agggaagtct gatggcagaa gtagaagagt tacgagtaca acctcaagat atcttagctg
     7561 agcaatccgt tttaggggct atctttattg atgagagtaa acttgttttt gtgcgagaat
     7621 acattgagtc tcgggacttt tttaagtatg cccatcgttt gattttccaa gccatggtcg
     7681 atttatccga tcgtggtgat gccatagatg caacaacggt tcgtactatc cttgataatc
     7741 aaggtgattt acagaatatt ggtggcttgt cttacttggt tgagattgtt aattctgtgc
     7801 caacttctgc taatgcggag tattatgcta agattgttgc agaaaaagca atgctacgtc
     7861 gtttaattgc caagttgaca gagtctgtca accaagctta cgaagcgtca caaccagctg
     7921 atgaaattat tgctcaggca gaaaaagggt tgattgatgt cagtgaaaat gcaaatcgaa
     7981 gcgggtttaa gaacattcga gatgtgttga atctcaactt tggaaatctg gaagctcgct
     8041 cgcaacaaac gaccgatatt acaggtattg cgacaggtta tcgtgatttg gatcatatga
     8101 caacaggact tcatgaggag gagttgatta tcttagcagc tcgtccagca gttggtaaga
     8161 cagcatttgc cttgaatatc gctcagaaca ttgggactaa gttggacaaa acggttgcta
     8221 ttttttcact cgaaatgggt gcggaaagct tggtagatcg tatgttagct gcagaaggct
     8281 tagtggagtc acattctatc cgtacagggc aattgacaga tgaggagtgg caaaaatata
     8341 ctattgctca gggtaatcta gctaacgcca gtatctatat cgatgatacg ccaggtattc
     8401 ggattacaga gattcgttct cgttctcgta aattggctca agaaactgga aatcttggtt
     8461 tgattttgat agactatttg caacttatca cgggaactgg tcgagaaaat cgtcaacaag
     8521 aagtttctga aatttctcgt cagttgaaaa tactagccaa ggaattgaag gttccagtaa
     8581 tcgctctgag tcagctttct cgtggtgtag aacaacgtca ggacaagaga ccggtcttgt
     8641 ctgatattcg tgaatctggg tctattgagc aggacgctga tatcgtagct tttctctatc
     8701 gcgatgacta ctatgaacgt ggtggtgaag aagaggaggg tatcccaaat aataaggtgg
     8761 aagttattat cgagaaaaac cgtagtggag ctcgtggaac agtggaattg attttccaaa
     8821 aagaatacaa taaattttca agtatctcaa agagggaggc ataagatgtc agatgcattt
     8881 acagatgtag ccaagatgaa aaaaatcaaa gaagaaatca aggcacatga gggacaagtc
     8941 gtagaaatga ctttggagaa tggtcgtaag cgccaaaaaa atagattggg taagctaatt
     9001 gaagtttatc catctctatt tattgtggag tttggggatg tggaaggaga taaacaagtt
     9061 aatgtttacg ttgaatcctt tacttactca gatattctta cagaaaagaa tttgattcat
     9121 tatcttgact aaagtgagaa attttctcac tttttctttt ttctccgaat aatttaggtg
     9181 aaggcaatca tcgctttata ttatttttca aggaggaaga atgaaaattt taccgtttat
     9241 agcaagagga acaagttatt acttgaagat gtcagttaaa aagcttgttc cttttttagt
     9301 agtaggattg atgctagcag ctggtgatag tgtctatgcc tattccagag gaaatggatc
     9361 gattgcgcgt ggggatgatt atcctgctta ttataaaaat gggagccagg agattgatca
     9421 gtggcgcatg tattctcgtc agtgtacttc ttttgtagcc tttcgtttga gtaatgtcaa
     9481 tggttttgaa attccggcag cttatggaaa tgcgaatgaa tggggacatc gtgctcgtcg
     9541 ggaaggttat cgtgtagata atacaccgac gattggttcc attacttggt ctactgcagg
     9601 aacttatggt catgttgcct gggtgtcaaa tgtaatggga gatcagattg agattgagga
     9661 atataactat ggttatacag aatcctataa taaacgaatt ataaaagcaa acacgatgac
     9721 aggatttatt cattttaaag atttggatgg tggcagtgtt gggaatagtc aatcctcaac
     9781 ttcaacaggc ggaactcatt attttaagac caagtctgct atcaaaactg aacccctagt
     9841 tagtgcaact gtgattgatt actattatcc tggagagaag gttcattatg atcaaattct
     9901 cgaaaaagac ggctacaagt ggttgagtta tactgcctat aatggaagct atcgttatgt
     9961 tcaattggag gctgtgaata aaaatcctct aggtcattct gttctttctt caacaggtgg
    10021 aactcattat tttaagacca agtctgctat caaaactgaa cccctagtta gcggaactgt
    10081 gattgattac tattatcctg gggagaaggt tcattatgat cagatacttg aaaaagacga
    10141 ctataagtgg ttgagttata cggcttataa cggaagtcgt cgctatatac agctagaggg
    10201 agtgacttct tcacaaaatt atcagaatca atcaggaaat atctctagct atggatccaa
    10261 taatagttca actgtcggtt ggaagaaaat aaatggtagt tggtatcatt tcaaatcaaa
    10321 tggttctaaa tcaacaggat ggctgaaaga cggttctagc tggtattatt tgaaattatc
    10381 tggtgaaatg cagacaggat ggttaaagga gaatggctcg tggtattatc tgggtagttc
    10441 aggggcaatg aaaacaggct ggtaccaggt ctctggtaag tggtattatt cttactcttc
    10501 aggcgcctta gctgttaata cgacggtgga tggctacaga gtaaacagtg atggagaacg
    10561 agtatagaaa attggagtag gagaaatttc ctgctctttt ctgtaagcag tttccttgac
    10621 atttttctta ttttgcgcta tcatatatac atataatata tgggagtctg tgtacagtct
    10681 gagaggaagt gttaaacttc gaccgcacct gatctgggta atgccagcgg agggaacgat
    10741 acttagtcta attttgcacc ttttccatgt atggtaaagg tttttctttt tttaaaagga
    10801 aaacgagaag aggaggttct tatgaaagca agcattgcct tgcaagtttt acccctagca
    10861 caggggattg atcggatagc tgttattgat caggtcattg cttatctgca aactcaagaa
    10921 gtgacgatgg tagtgacacc atttgaaacg gtcttggaag gggagtttga tgagcttatg
    10981 cgcattctaa aagaagcgct ggaagtggca gggcaggagg cagacaatgt ctttgccaat
    11041 gtcaaaataa atgtaggaga gattttaagt attgatgaga aacttgaaaa gtatactgag
    11101 acgacacatt agtctattgg gctttctcgg agtattgtca atctggcagt tagcaggttt
    11161 tcttaaactt ctccccaagt ttatcctgcc gacacctctt gaaattctcc agccctttgt
    11221 tcgtgacaga gaatttctct ggcaccatag ctgggcgacc ttgagagtgg ctttactggg
    11281 gctgattttg ggagttttga ttgcctgtct tatggctgtg ctcatggata gtttgacttg
    11341 gttcaatgac ctgatttacc ctatgatggt ggtcattcag accattccga ccattgccat
    11401 agctcctatc ctggtcttgt ggctaggtta tgggattttg cccaagattg tcttgattat
    11461 cttaacaaca acctttccca tcatcgttag tattttggac ggttttaggc attgtgacaa
    11521 ggatatgctg accttgttta gtctgatgcg agcaaaccct tggcaaatcc tgtggcattt
    11581 taaaatcccg gttagcctgc cttactttta tgcaggtctg agggtcagtg tctcctacgc
    11641 ctttatcaca actgtggtat ctgagtggtt gggaggtttt gaaggtcttg gtgtttatat
    11701 gattcagtct aaaaaactgt ttcagtatga taccatgttt gccattatta ttctggtgtc
    11761 gattatcagt cttttgggta tgaagctggt cgatatcagt gaaaaatatg tgattaaatg
    11821 gaaacgtact taaaatagag cgtttttgaa aaagaaaaga ggaaatcaaa atgaagaaaa
    11881 catggaaagt gtttttaacg cttgtaacag ctcttgtagc tgttgtgctt gtggcctgtg
    11941 gtcaaggaac tgcttctaaa gacaacaaag aggcagaact taagaaggtt gactttatcc
    12001 tagactggac accaaatacc aaccacacag ggctttatgt tgccaaggaa aaaggttatt
    12061 tcaaagaagc tggagtggat gttgatttga aattgccacc agaagaaagt tcttctgact
    12121 tggttatcaa cggaaaggca ccatttgcag tgtatttcca agactacatg gctaagaaat
    12181 tggaaaaagg agcaggaatc actgccgttg cagctattgt tgaacacaat acatcaggaa
    12241 tcatctctcg taaatctgat aatgtaagca gtccaaaaga cttggttggt aagaaatatg
    12301 ggacatggaa tgacccaact gaacttgcta tgttgaaaac cttggtagaa tctcaaggtg
    12361 gagactttga gaaggttgaa aaagtaccaa ataacgactc aaactcaatc acaccgattg
    12421 ccaatggcgt ctttgatact gcttggattt actacggttg ggatggtatc cttgctaaat
    12481 ctcaaggtgt agatgctaac ttcatgtact tgaaagacta tgtcaaggag tttgactact
    12541 attcaccagt tatcatcgca aacaacgact atctgaaaga taacaaagaa gaagctcgca
    12601 aagtcatcca agccatcaaa aaaggctacc aatatgccat ggaacatcca gaagaagctg
    12661 cagatattct catcaagaat gcacctgaac tcaaggaaaa acgtgacttt gtcatcgaat
    12721 ctcaaaaata cttgtcaaaa gaatacgcaa gcgacaagga aaaatggggt caatttgacg
    12781 cagctcgctg gaatgctttc tacaaatggg ataaagaaaa tggtatcctt aaagaagact
    12841 tgacagacaa aggcttcacc aacgaatttg tgaaataatg acagaaatta gactagagca
    12901 cgtcagttat gcctatggtc aggagaggat tttagaggat atcaacctac aggtgacttc
    12961 aggcgaagtg gtttccatcc taggcccaag tggtgttgga aagaccaccc tctttaatct
    13021 aatcgctggg attttagaag ttcagtcagg gagaattgtc cttgatggtg aagaaaatcc
    13081 caaggggcgc gtgagttata tgttgcaaaa ggatctgctc ttggagcaca agacggtgct
    13141 tggaaatatc attctgcccc tcttgattca aaaggtggat aaggcagaag ctatttcccg
    13201 agcggataaa attcttgcga ccttccagct gacagctgta agagacaagt atcctcatga
    13261 acttagcggt gggatgcgcc agcgtgtagc cttactccgg acctaccttt ttgggcacaa
    13321 gctctttctc ttagatgagg cctttagcgc cttggatgag atgacaaaga tggaactcca
    13381 cgcttggtat cttgagattc acaaacagtt gcagctaaca accctgatca tcacgcatag
    13441 tattgaggag gccctcaatc tcagcgaccg catctatatc ttgaaaaatc gccctgggca
    13501 gattgtttca gaaattaaac tagattggtc tgaagatgag gacaaggaag tccaaaagat
    13561 tgcctacaaa cgtcaaatct tggcagaatt aggcttagat aagtagaaaa atagggagtt
    13621 ggtgaagatt atcctttacc agcgcccttt ttcttttaaa aatgagaaaa tttcggtata
    13681 atagtcaaac aaggtcaagg tttaaagaga gaggtgggtt tgttatgaga tttaaaaata
    13741 catcggatca tattgaggcc tacatcaagg cgattttaga tcaatctggt atcgtggagt
    13801 tgcaacggag tcagttggca gatacctttc aggttgttcc tagtcagatt aactacgtga
    13861 tcaagacacg ctttacggaa agtagaggct acttggttga aagtaagcgt ggtggcggag
    13921 gctacattcg tataggacgg attgagtttt ctagtcatca tgaaatgctc cgggagctgc
    13981 tttactcgat tggtgagcga gtcagtcaag aaatttatga ggatattctc cagcttttgg
    14041 ttgagcagaa attgatgacc aagcaggaga tgaatttgct agaatcagta gctttggatc
    14101 gcgttttagg agaagaagct ccagttgttc gagcaaacat gctacgtcag gtcatacaag
    14161 aggtagatag aaaagggaag taagatgaac tattcaaaag cattgaatga atgtatcgaa
    14221 agtgcctaca tggttgctgg acattttgga gctcgttatc tagagtcttg gcacttgttg
    14281 attgccatgt ctaatcacag ttatagtgta gcaggggcaa ctttaaatga ttatccgtat
    14341 gagatggacc gtttagaaga ggtggctttg gaactgactg aaacggacta tagccaggat
    14401 gaaaccttta cggaattgcc gttctcccgt cgtttgcagg ttctttttga tgaagcagag
    14461 tatgtagcgt cagtggtcca tgctaaggta ctagggacag agcacgtcct ctatgcgatt
    14521 ttgcatgatg gcaatgcctt ggcgactcgt atcttggaga gggctggttt ttcttatgaa
    14581 gacaagaaag atcaggtcaa gattgctgcc cttcgtcgaa atttagaaga acgtgcaggc
    14641 tggactcgtg aagatctcaa ggctttacgc caacgccatc gtacagtagc tgacaagcaa
    14701 aattctatgg ccaatatgat gggcatgccg cagactccta gtggtggtct cgaggactat
    14761 acgcatgatt tgacagagca agcgcgttct ggcaagttag aaccagtcat cggtcgggac
    14821 aaggaaatct cacgtatgat tcaaatcttg agccgtaaga ctaagaacaa ccctgtcttg
    14881 gttggggatg ctggtgtcgg gaaaacagct ctggcgcttg gtcttgctca gcgtattgct
    14941 agtggtgacg tgtctgcgga aatggctaag atgcgcgtgt tagaacttga tttgatgaat
    15001 gtcgttgcag ggacacgctt ccgtggtgac tttgaagaac gcatgaataa tatcatcaag
    15061 gatattgaag aagatggcca agtcatcctc tttatcgatg aactccacac catcatgggt
    15121 tctggtagcg gaattgattc gactctggat gcggccaata tcttgaaacc agccttggcg
    15181 cgtggaactt tgagaacggt tggtgccacc actcaggaag aatatcaaaa acatatcgaa
    15241 aaagatgcgg ctctttctcg tcgtttcgct aaagtgacga ttgaagaacc aagtgtggca
    15301 gatagtatga ctattttaca aggtttgaag gcgacttatg agaaacatca ccgtgtacaa
    15361 atcacagatg aagcggttga aacagcggtt aagatggctc atcgttattt aaccagtcgt
    15421 cacttgccag actctgctat cgacctcttg gacgaagcag cagcaacagt gcaaaataag
    15481 gcaaagtatg taaaagcaga cgattcagat ttgagtccag ctgacaaggc cctgatggat
    15541 ggcaagtgga aacaggcagc ccagctaatc gcaaaagaag aggaagtacc tgtctacaaa
    15601 gacttggtga cagagtctga tattttgacc accttgagtc gcttgtcagg aatcccagtt
    15661 caaaaactga ctcagactga cgctaagaaa tatctaaatc ttgaagcaga actccataaa
    15721 cgggttatcg gtcaagatca agctgtttca agtattagtc gtgcgattcg ccgtaatcag
    15781 tcaggaattc gcagtcacaa gcgtccgatc ggttccttta tgttcctagg acccacaggt
    15841 gtcggtaaga ccgagttggc caaggctttg gcagaagttc tctttgatga cgaatcagcc
    15901 cttatccgct ttgatatgag tgagtatatg gagaaattcg cagccagccg tctaaatgga
    15961 gctcctccag gctatgtggg ttacgaagaa ggtggggagt tgacagagaa ggttcgcaat
    16021 aaaccctatt ccgttctcct ctttgatgag gtagagaagg ctcacccaga tatctttaat
    16081 gttctcttgc aggttctgga tgacggtgtc ttgacagata gcaagggacg caaggtcgat
    16141 ttttcaaata ccattatcat tatgacatcg aacctaggtg cgactgccct tcgtgatgat
    16201 aagactgttg gttttggggc taaggatatt cgttttgacc aggaaaatat ggaaaaacgc
    16261 atgtttgaag aactgaaaaa agcttataga ccggaattca tcaaccgtat tgatgagaag
    16321 gtggtcttcc atagcctatc tagtgaccat atgcaggaag tggtgaagat tatggtcaag
    16381 cctttagtgg caagtttggc tgaaaaaggc attgacttga aattacaagc ttcagctctg
    16441 aaattgttag caaatcaagg atatgaccca gagatgggag ctcgcccact tcgcagaacc
    16501 ctgcaaacag aagtggagga caagttggca gaacttcttc tcaagggaga tttagtggca
    16561 ggcagcacac ttaagattgg tgtcaaagca ggccagttaa aatttgatat tgcataaaag
    16621 aataaaagta ccagcatctg accataagtc acagtggagt gaaactcaat gaaaatcaaa
    16681 gagcaaacta ggcagctagc cgcaggttgc tcaaaacact ggtttgaggt tgcagataga
    16741 gctgacgtgg tttgaagaga ttttcgaaga gtatgaaact aaaacctata gcttctaaac
    16801 gattcgtggt tttcaccatt caacacaaaa ttcatatgtt tattaccctc cgtcgtattt
    16861 gtcttagagc gtgtgtagta gaaaaagagc agtcttatct gaaattttta ttctttcaaa
    16921 agagacctgt ttcttttttg catgtcaaat ccgttctagc tggtatttga aaaatcaaac
    16981 taatattcaa tgaaaatcaa agagcaaact aggtagctag ccgcaggttg ctcaaaacac
    17041 tgttttgagg ttgtagatag agctaacgtg gtttgaagag attttcgaag agtataagct
    17101 gcaagatgaa tgattttctt gtattgacgt tgttgttgac aaaaagtagc ggataaatga
    17161 aatccattcc attatcatag atgataggct ggtaggaaat tttcaaatag catacaggaa
    17221 atagatgtat ggagttctgg tagtagaaag ggagagagat gaacatttta gttgcagatg
    17281 acgaggaaat gattagagaa ggaattgcag catttctgac agaagagggt tatcatgtca
    17341 ttatggctaa ggatggacaa gaggtcttgg aaaaatttca agatctccct atccatctca
    17401 tggtactgga tttaatgatg cctaggaaga gtggttttga agtgttaaaa gaaatcaatc
    17461 aaaagcacga tattcctgtc atcgtcttga gtgctctggg agatgaaact actcagtcac
    17521 aggtatttga tctctatgct gatgatcatg tgacaaaacc tttttctttg gtactgcttg
    17581 tcaagcgtat taaggcgctt atcagacgtt actacgtcat agaggatatt tggcgatatc
    17641 aggatgtaac agtggatttt acctcttaca aagcacatta taaaaatgaa gaaattgatc
    17701 tcaaaccaaa ggaattactg gtactaaagt gtttgattca gcataaaaat caagttttaa
    17761 gtagagagca gatattggaa gaaatttcaa aagatgtagc tgatttacct tgtgataggg
    17821 tcgttgatgt ctatattcgt actcttcgca aaaaattagc tttagattgt atcgtgactg
    17881 tgaaaaatgt tgggtataag attagcttat gataaaaaat cctaaattat taaccaagtc
    17941 ttttttaaga agttttgcaa ttctaggtgg tgttggtcta gtcattcata tagctattta
    18001 tttgaccttt cctttttatt atattcaact ggagggggaa aagtttaatg agagcgcaag
    18061 agtgtttacg gagtatttaa agactaagac atctgatgaa attccaagct tactccagtc
    18121 ttattcaaag tccttgacca tatctgctca ccttaaaaga gatattgtag ataagcggct
    18181 ccctcttgtg catgacttgg atattaaaga tggaaagcta tcaaattata tcgtgatgtt
    18241 agatatgtct gttagtacag cagatggtaa acaggtaacc gtgcaatttg ttcacggggt
    18301 agatgtctac aaagaagcaa agaatatttt gcttttgtat ctcccatata catttttggt
    18361 tacaattgct ttttcctttg ttttttctta tttttatact aaacgcttgc tcaatcctct
    18421 tttttacatt tcagaagtga ctagtaaaat gcaagatttg gatgacaata ttcgttttga
    18481 tgaaagtagg aaagatgaag ttggtgaagt tggtgaagtt ggaaaacaga ttaatggtat
    18541 gtatgagcac ttgttgaagg ttattcatga gttggaaagt cgtaatgagc aaattgtaaa
    18601 attgcaaaat caaaaggttt cctttgtccg cggagcatca catgagttga aaaccccttt
    18661 agccagtctt agaattatcc tagagaatat gcagcataat attggagatt acaaagatca
    18721 tccaaaatat attgcaaaga gtataaataa gattgaccag atgagccact tattggaaga
    18781 agtactggag tcttctaaat tccaagagtg gacagagtgt cgtgagacct tgactgttaa
    18841 gccagtttta gtagatattt tatcacgtta tcaagaatta gctcattcaa taggtgttac
    18901 aattgaaaat caattgacag atgctactag ggtcgtcatg agtcttaggg cattggataa
    18961 ggttttgaca aacctgatta gtaatgcaat taaatattca gataaaaatg ggcgtgtaat
    19021 catatccgag caagatggct atctctctat caaaaataca tgtgcgcctc taagtgacca
    19081 agaactagaa catttatttg atatattcta tcattctcaa atcgtgacag ataaggatga
    19141 aagttccggt ttgggtcttt acattgtgag taatatttta gaaagctatc aaatggacta
    19201 tagttttctc ccttatgaac acggtatgga atttaagatt agcttgtaga cagattagtt
    19261 ttttattaaa gttcacatag ggttaacata agtgtattat tctttatata gataaaaata
    19321 tagataaaag aaaggatact aatatggtat tagcgattat tttagtaaca ttccttattc
    19381 gattgatttt tttaaagcgt tcgatagaga atgagaaacg aatccttagc aatggcggga
    19441 aagaatttgg agttgagaat acaaaacgat taacgttggc tcatattgtt ttttatctct
    19501 cttgcctcgt tgaagcaatg gtgcataaga caatgtttga tggtatgagc atggttggtt
    19561 tagtcttgct tattttttct atgctgatgt tgatgttggt gattcacttg ttgggagata
    19621 tttggacagt gaagcttatg cttgtcaata atcacaaata tgtagatcat atcttgttta
    19681 ggacagtaaa acatcctaat tactttttaa atattcttcc tgagttgatt ggcttgacct
    19741 tgttgagtca tgcttatgtg acttttgttt tagtttttcc agtttatgca gttattttgt
    19801 atcgacgaat agctgaagag gaaaagctat tacatgaagt tataatccca aatggaagaa
    19861 tgaaaggtta aaaataaaat acccaattta atatacagtt catattggaa gggtatactg
    19921 aagatgaata aaatattata ggatatcgat atgtttaaat caaattatga aagaaaaatg
    19981 tgctattcta ttcgaaaatt tagtatagga gtagctagtg tagctgttgc cagtcttgtt
    20041 atgggaagtg tggttcatgc gacagagaac gagggaacta cccaagcacc cacttcttct
    20101 aataggggaa atgaaagtca ggcagaacaa cgtagagaac tcgatttaga acgagataag
    20161 gtaaagaaag aggtcaggga atataaagaa aaaaaagtga aagagctcta ttcaaaatca
    20221 actaaaagtc gacataagaa gactgtagat atagttaaca agttgcaaaa cattaataac
    20281 gagtatttga ataaaataat tcaatcaacc tcaacatacg aagaactaca gaaactgatg
    20341 atggagagtc aatcagaagt agataaagct gtgtctgaat ttgaaaagga cttatcttct
    20401 tcgtcaagtt caggctcttc cacggaaccg gaagcttcag atacagcgaa gccaaacaag
    20461 ccgacagaac tagaaaaaaa ggtagcagaa gctcagcaga aggttgaaga agctgagaaa
    20521 aaagccaagg atcaaaaaga agaagattac cgtaactacc caaccattac ttacaaaacg
    20581 cttgaacttg aaattgctga gttcgatgtg aaagttaaag aagcggagct tgaactatta
    20641 aaagtgaaag ctaaggaatc tcgagacgag aaaaaaatta agcaagcaga agcggaagtt
    20701 gagagtaaac aagctgaggc tacaaggtta aaaaaaatca agacagatcg taaaaaagca
    20761 gaagaagaag ctaagttgaa ggaagctgtt gaaaagaatg cagcgacttc agagcaaggt
    20821 aaaccaaaga ggcgggtaaa acgaggagct cttggagagc aagcaacacc tgataaaaaa
    20881 gattattttg aaaaagactt ccgtccagct ttcaataaaa accagcagat ggtagccatt
    20941 caagaatcct tgaacaaact agatgatgaa acaaaaactg ttccagatgg ggctaaactc
    21001 acaggagaag ctggaaatgc ctataatgag gtcagagatt atgcaataaa agttgtttct
    21061 gaaaacaaga aacttctatc acagacagca gtgacaatgg atgaactggc aatgcaatta
    21121 accaaattga acgatgccat gtctaaattg aaagaagcta aagcgaaatt ggtaccagag
    21181 gttaaaccgc agccggaaaa tccagagcat caaaaaccaa caactccagc tccggatacc
    21241 aaaccaatcc ctcaaccaga gggtaagaaa ccaagcgtac cagatattaa tcaggaaaaa
    21301 gaaaaagcta agcttgctgt agcaacctac atgagcaaga ttttagatga tatacaaaaa
    21361 catcatctgc agaaagaaaa acatcgtcag attgttgctc ttattaagga gcttgatgag
    21421 tttaaaaagc aagctctttc tgaaattgat aatgtaaata ccaaagtaga aattgaaaat
    21481 acagtccaca agatatttgc agacatggat gcagttgtga ctaaattcaa aaaaggctta
    21541 actcaggaca caccaaaaga accagataac aaaaagccat ctgctccaaa accag
//
LOCUS       DAVKWS010000033        21315 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKWS010000033 DAVKWS010000000
VERSION     DAVKWS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.033"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            50..484
                     /locus_tag="VW852_001331"
     CDS             50..484
                     /locus_tag="VW852_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0272472.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLSMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            496..1686
                     /locus_tag="VW852_001332"
     CDS             496..1686
                     /locus_tag="VW852_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV0272473.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1697..2188
                     /locus_tag="VW852_001333"
     CDS             1697..2188
                     /locus_tag="VW852_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0272474.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2203..2982
                     /locus_tag="VW852_001334"
     CDS             2203..2982
                     /locus_tag="VW852_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0272475.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFVFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            2969..3787
                     /locus_tag="VW852_001335"
     CDS             2969..3787
                     /locus_tag="VW852_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0272476.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3787..4080
                     /locus_tag="VW852_001336"
     CDS             3787..4080
                     /locus_tag="VW852_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0272477.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4102..6003
                     /locus_tag="VW852_001337"
     CDS             4102..6003
                     /locus_tag="VW852_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV0272478.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKSYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6063..7064
                     /locus_tag="VW852_001338"
     CDS             6063..7064
                     /locus_tag="VW852_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0272479.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7174..7509)
                     /locus_tag="VW852_001339"
     CDS             complement(7174..7509)
                     /locus_tag="VW852_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272480.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7520..7762)
                     /locus_tag="VW852_001340"
     CDS             complement(7520..7762)
                     /locus_tag="VW852_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272481.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDKD"
     gene            complement(7773..8315)
                     /locus_tag="VW852_001341"
     CDS             complement(7773..8315)
                     /locus_tag="VW852_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV0272482.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKDPDED"
     gene            complement(8331..8525)
                     /locus_tag="VW852_001342"
     CDS             complement(8331..8525)
                     /locus_tag="VW852_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272483.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8691..9641
                     /gene="rsmH"
                     /locus_tag="VW852_001343"
     CDS             8691..9641
                     /gene="rsmH"
                     /locus_tag="VW852_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV0272484.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQARDLKPIETTTELAELIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMEMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9653..9970
                     /gene="ftsL"
                     /locus_tag="VW852_001344"
     CDS             9653..9970
                     /gene="ftsL"
                     /locus_tag="VW852_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV0272485.1"
                     /translation="MAERMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9974..12226
                     /gene="pbpX"
                     /locus_tag="VW852_001345"
     CDS             9974..12226
                     /gene="pbpX"
                     /locus_tag="VW852_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV0272486.1"
                     /translation="MKWTKKVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGSKGNGITY
                     ANMMAIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPSGEYFNSSELKIADVTIRDWDVNDGLTTGRMMTFLQGF
                     ALSSNVGMSLLEQKMGDTTWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMILVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     NYIFSAVSMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELAFEGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12228..13208
                     /locus_tag="VW852_001346"
     CDS             12228..13208
                     /locus_tag="VW852_001346"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV0272487.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFVFSLFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAVIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMTLHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13345..13506
                     /locus_tag="VW852_001347"
     CDS             13345..13506
                     /locus_tag="VW852_001347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272488.1"
                     /translation="MLNENQEQTRKLALGSSKHGFEVVDKAELMWFEEIFEEYKKYWS
                     VKVKTLDLF"
     gene            13763..15868
                     /locus_tag="VW852_001348"
     CDS             13763..15868
                     /locus_tag="VW852_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0272489.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPDEF
                     AHYRATGQLPGNAETDGQMQQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENIQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     EAEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLEHKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGPTGVGKTELAKQLALDMFGTKEAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGGLVIREKA"
     gene            16321..16542
                     /locus_tag="VW852_001349"
                     /pseudo
     CDS             16321..16542
                     /locus_tag="VW852_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(16828..17310)
                     /locus_tag="VW852_001350"
     CDS             complement(16828..17310)
                     /locus_tag="VW852_001350"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV0272490.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(17405..18889)
                     /locus_tag="VW852_001351"
     CDS             complement(17405..18889)
                     /locus_tag="VW852_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV0272491.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            19042..20649
                     /locus_tag="VW852_001352"
     CDS             19042..20649
                     /locus_tag="VW852_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV0272492.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            20808..20981
                     /locus_tag="VW852_001353"
     CDS             20808..20981
                     /locus_tag="VW852_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV0272493.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACDQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(20974..>21315)
                     /locus_tag="VW852_001354"
     CDS             complement(20974..>21315)
                     /locus_tag="VW852_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0272494.1"
                     /translation="SLVAGLTNGELIVPMTYEETMTSDFFEAWFQKFFLPTLTTPSVI
                     IMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTF
                     YEALLSFSCFN"
BASE COUNT         6447 a         3829 c         4860 g         6179 t
ORIGIN      
        1 atggggaata taaagtaaac cggtaaacct aaacaagaaa ggagaaaaga tgaaaattgt
       61 acttgtaggg catggacatt ttgctacagg gatttatagt tctttacaat tgattgcagg
      121 caatcaagaa aatgtggagg cgattgactt tgtggaagga atgtcagcag atgaactcaa
      181 gcaaaaaatc ttacttgcaa tttcaaatga agaagaagtt ttaatcctaa gtgatctctt
      241 gggaggatcg ccattcaagg tttcttctac cataatggga gaaaatccag ccaaaacaat
      301 gaatgttctc tcgggtttga acttatccat gttaatggaa gcagtctttg ctagaatggc
      361 tcatagcttt gatgaggttg ttaataaatc agtagtggcg gcccagggcg gagtcgtaaa
      421 tggtaaagaa ttgttttcaa cggatgcaga ggaagaggaa gaagatttcg aatcgggtat
      481 ttaaagggtg aaagaatgat aaaaaaggtt acgattgaaa aaataaagtc gcctgagcgc
      541 ttcttagaag taccacttct gacgaaagaa gaagtcggcc aggcaatcga taaggttatt
      601 cggcagttag aactcaacct tgactatttc aaggaagatt tcccgacgcc agctaccttt
      661 gataatgtct atccaatcat ggataacacg gaatggacca atggtttctg gacaggagaa
      721 ctgtggttgg cttatgaata cagtcaacag gatgcattta aaaacatcgc tcataaaaat
      781 gttctttctt tcctggatcg tgtcaataag agagtagaat tggatcatca tgatctcggc
      841 ttcttgtaca caccgtcttg tatggctgaa tataagataa atggagatgg agaggctaga
      901 gaagcaacct tgaaagctgc agataagttg attgaacgct atcaagaaaa aggtggtttt
      961 attcaagctt ggggagactt gggcaagaaa gagcattacc gtttgattat cgactgcttg
     1021 ctcaatatcc aactcttatt ctttgcttat caagaaacag gcgatcaaaa atactacgat
     1081 attgcagaaa gccatttcta tgcttcagct aataatgtaa tccgtgatga cgcttcgtcc
     1141 ttccacacct tctattttga tcctgagaca ggtcaaccct ttaaaggtgt aacgagacaa
     1201 gggtatagtg atgattcatg ctgggcacgt ggtcaatcat ggggagtcta tggtattcct
     1261 ttgacttatc gtcacttaaa agacgagtcc tgctttgact tgtttaaggg tgtgaccaat
     1321 tatttcttga atcgtctgcc aaaagatcat gtgtcttatt gggatttgat ttttaatgat
     1381 ggtagtgatc aatcacgaga ttcttcagca acagctatcg ccgtctgtgg gattcatgaa
     1441 atgctaaaac atctcccaga ggtggatgct gacaaagata tttataaaca tgctatgcat
     1501 gccatgcttc gttccttgat cgaacattat gcaaatgatc aatttacccc tggtgggaca
     1561 agtctcctcc acggtgtgta ctcatggcat tcaggtaaag gagtggatga aggcaatatc
     1621 tggggtgact actattacct agaagccctt atccgtttct acaaagactg gaacctatat
     1681 tggtaggagg agaaatatga caatgccaaa tattattatg acccgtatcg atgaacggtt
     1741 gattcatgga caaggacaac tttgggtaaa atacctaggt tgtaatacgg tcattgttgc
     1801 caatgatgaa gtaagcacgg acaagatgca acaaactctg atgaaaacag ttgtgccaga
     1861 ctcagttgcc atgcgtttct tccctttgca aaaggtgatt gatatcattc acaaggctaa
     1921 tcctgctcaa acgatcttta tcgttgtaaa gaatgtgaag gatgctttaa ccttggtaga
     1981 aggtggtgtc cctatcaaag aaatcaatat tgggaacatt cacaatgcta ctggtaaaga
     2041 gcaagtgaca cgctccatct tcctgggtga agaggacaag gtggccctca aggaattgag
     2101 ccaaactcat caagtaacat ttaatacgaa aacaactcca acaggaaatg atggagctgt
     2161 tcaagtcaac attatggact atatttaaaa gaggagatcg ttatgtcgat taatgtattt
     2221 caagcgattt taattggatt atggacagct ttctgtttta gtggaatgct gttaggaatt
     2281 tacaccaata gatgtattgt tctgtcattt ggtgtcggaa ttattctagg tgatctgcct
     2341 actgctcttg caatgggagc tattggtgaa ttggcttata tgggattcgg tgttggtgct
     2401 ggaggtactg ttccaccaaa cccaatcgga cctggtatct ttggtacctt gatggctatc
     2461 actagtgctg gtaaagtcag tccagaagcg gctcttgccc tctctactcc gattgctgtg
     2521 gcgattcaat tcttacaaac tttcgcctac actgtacgtg ctggtgcgcc tgaaacggcg
     2581 atgaagcact tgaaaaacca taatttgaag aaattcaagt tcactctaaa tgcaacaatt
     2641 tggttgtttg tctttattgg atttaccttg ggttgcttgg gcgccctttc aatggatacc
     2701 ttgttgaaac tcgtagacta cattccaccg gtattgctta caggtttgac agttgctggt
     2761 aaaatgctcc cagctatcgg ttttgcgatg atcttgtcag tgatggctaa gaaagagttg
     2821 attccctttg tcttgttggg atatgtttgt gcagcttatc taaacatccc aacaattggt
     2881 attgcaattg taggtactat ctttgctttg attgaatttt ataacaagcc aaaaacagcg
     2941 gatcatgtgg tagaggagga agcacacgat gactggatct aacaaattaa caaaacgtga
     3001 ttatcttaaa acgtctttgc gggcattctt ttgtcaaaat ggatttaact atagtaacta
     3061 tcaagggttg ggatatgcca atgtgatgta tcctgctttg aaaaaacact atggagagga
     3121 tcaggaaggt ttctaccaag ccttggaaga aaactgtgaa ttctataata ccaatccaca
     3181 cttcctgcct tttattacca gcttgcacct tgtaatgttg gaaaatggcc gaccaacaaa
     3241 agaaacacgt agcatcaaga tggccttgat gggaccatta gcaggtattg gggattctct
     3301 ctctcaattc tgtttagctc cattgttctc aaccatcgca gcttcgtttg ctcaagaagg
     3361 cttggttgtc ggtccaatct tgttcttcct tgcgatgaat acgattttaa cagcgattaa
     3421 attgtcaact ggtctgtatg gatacaaact aggaacaact gtgattgata aactaagcga
     3481 acagatggca acgatttctc gtattgccaa tattatcggt gtaaccgtaa ttgctggttt
     3541 ggcagcgaca tctgttaaga ttatggtgcc gattaccttt gctgcagggg aagttaaagc
     3601 agacgctaaa caaagtatcg taagtattca gggaatgctt gataaggttg ctccagctct
     3661 tctaccagcc ctatttacac ttttagttta ctacttgatc aaagaaaaga aatggacaac
     3721 atataaactc gttattttaa cagttatcat cggaattatc ggaagctggc ttaagattat
     3781 agcttaatgg atacaacatt gttttatgga atagtgattg tcttggcagt gagtcccctt
     3841 ttactgtcaa gctttcattc tattcgtcag caaaagttgc ttcgcaaaca gatggagcaa
     3901 cgacaagagt atttagcttc tttaacatct ggtgatgaag tgttgttatt gtctggaatt
     3961 catggaaaaa tcatttctat caaagatgac ttggtctcct tgcagattgc aaaaggtgtg
     4021 gtcatctatg tagaaaagga aagtgtaatg ggaaagacaa aagaactgct ttttaagtag
     4081 ttctttttct ggtaatagat aatgaaagaa gaaagaagac aattttttga aagaatcgat
     4141 ggaaaccaat gtcgtgatta tatcttgtcc cactgttcaa aagactatga gaaagtcaag
     4201 agttcccttg aacgcttgat ggacaatcgt tttatgtttg atagtccttg ggatatggag
     4261 ccttgttcaa aaatccatca aatccagccg atggtatggg atcaagtatt tgaagatgat
     4321 ccagaatggg cttatatgct caatcgacaa gaatatctct tgcagtttat gatagggtat
     4381 ctggtagaag gagataagag ctatattcaa aagtgcaagt tctttctatt tgattggatt
     4441 gagcaggtga gagaattttc tcctcaatcc ttgatgacta gaaccttgga tacgggtatt
     4501 cgttccttta cttggttgaa actactcttg ctcctcttga aatttgactt gctagaggag
     4561 aaagaactag agaaaatttt ggtcagtcta gaaaagcaga ttgactttat gaaaagccac
     4621 tatcgcgcca agtacaccct tagtaactgg gggattttac aaacaattcc gatgcttgct
     4681 atctattatt tcttttcaga taagatggat ctagaagaag cttaccattt tgcttcagag
     4741 gagttgaaac agcaaattga gacacagatt ttaggagatg gaagccagtt tgaacagtcg
     4801 attctctatc atgtagaggt ttataaagcc ttgctggatt tgtgtctctt gcttccagac
     4861 ttgcaagata gttaccgaga gttgctggaa aagatggcga cctatattca aatgatgaca
     4921 ggcttagatg gacgaacttt ggcttttggt gatagcgatt ctacagaaac gacagaaatg
     4981 ttgagcctgt ctgctgtggt tttgaacaag gaagaccttc ttaacggtct ggatgttaaa
     5041 gtcgatttgc ttagcctctt gttcctgggg cgagaaaagg tcaagcgact acaggaattt
     5101 gaaaggagag cttggcagcc taagtccatg atctttgaag actctggaca tgtctgcatt
     5161 aaggatgaac atcgttatct atttttcaaa aatggtccgc taggaagtgc ccatagccat
     5221 agtgacgaga atagtttttg cttacagtat caaggccaac ctattttcat agatgctggg
     5281 cgttattctt atcgggagat atatgaacgt tatctcttaa agagtgcttg gagtcattcg
     5341 acctgcattg tagatgggaa agctccggaa agaatcacgg gatcctggga atatgaatac
     5401 tatcctcact ccctgttttg tcaccataaa gaaagggagg gagtgcatta tattgagggg
     5461 gcttattggt cagcagaacc tgatttgcct tatcttcaca agagaaaaat cctcatgttg
     5521 gtagaggatg tctggctctt ggtagatgac atcaggtgtc aaggtcagca tgaggcgttg
     5581 actcagttta tccttgacaa ggatgtgacc tatcaagatg ggaaaatcaa tcagttgaga
     5641 ctatggagtg aagttgattt tgatttggaa gataccatca tttctcctaa atacaatgag
     5701 cttgaaagaa gtaacaaact caccaagcgc caattctttg agaatcagat gctggattat
     5761 accatcattg cgcatgagag ttttgaaatc atccgtcatt ctgtctacca gacagatgat
     5821 cgtgaagtgg aaaatgctct ggcttttgaa gtgaaaaatg atgaaacaga caagctgatt
     5881 ctgttattaa gcgaggatat tggtgtaggt gaaaaattgt gcctcgttga cggaacaaaa
     5941 atgcgtggaa aatgtctagt atatgataaa ataaatgaga gaatgattcg cttgcagtgc
     6001 tagaaatagg cattttgaat agtgaatatg ttataataat tattagtagg aggtgtttta
     6061 gattggagaa gaaactgacc ataaaagaca ttgcggaaat ggctcagacc tcgaaaacaa
     6121 ccgtgtcatt ttacctaaac gggaaatatg aaaaaatgtc ccaagagaca cgtgaaaaga
     6181 ttgaaaaagt tattcatgaa acaaattaca aaccgagcat tgttgcgcgt agcttaaact
     6241 ccaaacgaac aaaattaatc ggtgttttga tcggtgatat taccaacagt ttctcaaacc
     6301 aaattgttaa gggaattgag gatatcgcca gccagaatgg ctaccaggta atgataggaa
     6361 atagtaatta cagccaagag agtgaggacc ggtatattga aagcatgctt ctcttgggag
     6421 tagacggctt tattattcag ccgacctcta atttccgaaa atattctcgt atcatcgatg
     6481 agaaaaagaa gaaaatggtc ttttttgata gtcagctcta tgaacaccgg actagctggg
     6541 ttaaaaccaa taactatgat gccgtttatg acatgaccca gtcctgtatc gaaaaaggtt
     6601 atgagcattt tctcttgatt acagcggata cgagtcgttt gagtactcgg attgagcggg
     6661 caagtggttt tgtggatgct ttaacagatg ctaatatgcg tcacgccagt ctaaccattg
     6721 aagataagca tacgaatttg gaacaaatta aagaattttt acaaaaagaa atcgatcccg
     6781 atgaaaaaac tctggtattt atccctaact gttgggccct acctctagtc tttaccgtta
     6841 tcaaagagtt gaattataac ttgccgcaag ttgggttgat tggttttgac aatacggagt
     6901 ggacttgctt ttcttctcca agtgtttcga cgctggttca gccctctttt gaggaaggac
     6961 aacaggctac aaagattttg attgaccaga ttgaaggccg caatcaagaa gaaaggcaac
     7021 aagtcttgga ttgtagtgtg aattggaaag agtcgacttt ctaaaatgaa ggaaaatgac
     7081 ttgcaatctc tgttaagaaa taaaataatc ccacctagaa caagctaggt gggattattt
     7141 gcctatgaaa tgagaaatta tgggagcaag ctcctaaatc aactgttttt gatctacttc
     7201 tttaactact tgataaaagt tatagaagta ggccaaactt gaaatgatgg ttacgactag
     7261 gaatattgaa aatttccatt ggacagggtt ggttaaaagt tgtggaaagg atatgaggag
     7321 aaagaagagg gctgcgttga ggacaggtat ccgttttgat tgtattttct caagtccttt
     7381 attgagcgca ggaagaaaga ggagtaggag tagtaaaact gtatgagaaa cagctcctga
     7441 aataagggcg aagaaaagga aaatactgat aaaaacatga atgatcagta gtctagctag
     7501 tgatttcata aggcacctcc taatccttgt cttttttagc tcttgcaata cgaagtgagt
     7561 cgacaatatg tatcatcact ccgaaaaaga aagctcccag tatagtttta aaaatatgtt
     7621 ttgtatttag aagagaactg ataaaatttg gattttcact tgttagggta tcaatgagtg
     7681 gaattataaa aaatatcact gttccataaa tcgaacctgc tttcagacca ggataacgta
     7741 actgtctctt ttcttttttc atagggtacc tcctaatcct catccggatc tttcttagtt
     7801 tttgcaatgc gacgggagat gaggaactgt atgctcgctc cgaagaaaat agaaccgaga
     7861 atgcttgata caccatttct tatagtgaga agagaatgaa aatagtcctg accttcacct
     7921 atgagtatcc tgagaagagg agttataaaa aacatccata gaccaaagaa caaacctgct
     7981 ttcagacctg ggtaatgtag ttgcttactt tctttctcgc tcagcatgtc tggatcaata
     8041 gctgtaatcc ctgttttttt catttggtag gtgacatagc cagcagcgat gagggcaatc
     8101 actaaaatca gaggaggata gattagagcc acttcttgag ggtatttata ggccagaagg
     8161 agtggaataa gatttccgaa aatcatcaga taaaagagga cgataaaaac ttggttccca
     8221 atactatcgg cctcacgccg tttgtattcg tcaaggggac cagaaatacc gtatgtgcgt
     8281 ttgatcagtt tttcagtgaa ggtttctttt ttcatgagtt tgctcctttt ttaaaaatct
     8341 tcctcccaaa agagactgtt gaggtcagtt tggaggctgc gggcgagatt tagacagagt
     8401 tccaaggttg gattgtactt gtcgttttca atcatattga tagtctgtct cgagacaccg
     8461 atatccttgg cgagttcgag ctgggaaata cccaattcct tgcgaaattc tttcacacga
     8521 ttcatctgtt ctcctttctg atttatgtcg tatatatttg actatattat agtcttttaa
     8581 acataaagtg tcaagtattt ttgacatatt ttttgaagaa atagtagtct ccttgtccta
     8641 tttgtctgac aagtgcaagc tggtcggatt tgtggtaaaa tagataagat atgacaaaag
     8701 aatttcatca tgtaacggtc ttactccacg aaacgattga tatgcttgac gtaaaacctg
     8761 acggtatcta cgttgatgcg actttgggtg gagcaggcca tagcgagtat ttattaagta
     8821 aattaagtga aaaaggccat ctctatgcct ttgaccagga tcagaatgcc attgacaatg
     8881 cgcaaaaacg cttggcacct tacattgaga agggaatggt gacctttatc aaggataact
     8941 tccgtcattt acaggcacgt ttgcgcgaag ctggtgttca ggaaattgat ggaatttgtt
     9001 atgacttggg agtgtctagt cctcaattgg accagcgtga gcgtggtttt tcttataaaa
     9061 aggatgcgcc actggacatg cggatgaatc aggatgctag tctgacagcc tatgaagtgg
     9121 ttaatcacta tgactatcat gatttggttc gtattttctt caaatatggc gaggacaaat
     9181 tctctaaaca gattgcgcgt aagattgagc aagcgcgtga tctgaaacct atcgagacta
     9241 cgactgaatt ggcagaactt atcaagtcgg ccaaacctgc caaggaactc aagaagaagg
     9301 gccatcctgc taagcagatt ttccaggcta ttcgaattga agtcaatgat gaactgggag
     9361 cggcagatga gtccatccag caggctatgg agatgttggc tctggatggt agaatttcag
     9421 tgattacctt ccattcgcta gaagaccgct tgaccaagca attgttcaag gaagcttcaa
     9481 cagtggaagt tccaaaaggt ttgcccttca tcccagatga cctcaagccc aagatggaat
     9541 tggtgtcccg taagccaatc ttgccaagtg cagaagaatt agaagccaat aaccgctcgc
     9601 actcagccaa gttgcgcgtg gccagaaaaa ttcacaagta agagggaaaa agatggcaga
     9661 aagaatggaa aaaacaggtc aaatactaca gatgcaactt aaacggtttt cgcgtgtgga
     9721 aaaagctttt tacttttcca ttgctgtaac cactcttatt gtagccatta gtattatttt
     9781 tatgcagacc aagctgttgc aagtgcagaa tgatttgaca aaaatcaatg cgcagataga
     9841 agaaaagaag accgaattgg acgatgccaa gcaagaggtc aatgaactat tacgtgcaga
     9901 acgtttgaaa gaaattgcca attcacacga tttgcaatta aacaatgaaa atattcgaat
     9961 agcggagtaa gatatgaagt ggacaaaaaa agtaatccgt tatgcgacca aaaatcggaa
    10021 atcgccggct gaaaacagac gcagagttgg aaaaagtctg agtttattat ctgtctttgt
    10081 ttttgccgtt tttttagtca attttgcggt cattattggg acgggcactc gctttggaac
    10141 agatttagcg aaggaagcta agaaggttca tcaaaccacc cgtacagttc ctgccaaacg
    10201 tgggactatt tatgaccgaa atggagtccc gattgctgag gatgcaacct cctataatgt
    10261 ctatgcggtc attgatgaga actataagtc agcaacgggt aagattcttt acgtagaaaa
    10321 aacacaattt aacaaggttg cagaggtctt tcataagtat ctggacatgg aagaatctta
    10381 tgtaagagag caactctcgc aacctaatct caagcaagtt tcctttggtt cgaagggcaa
    10441 tgggattacc tatgccaata tgatggctat caaaaaagag ttggaaactg cagaggtcaa
    10501 ggggattgat tttacaacca gtcccaatcg tagttaccca aacggacaat ttgcttctag
    10561 ttttatcgga ctagctcagc tccatgaaaa tgaagatggc agcaagagct tgctgggaac
    10621 ctctggaatg gagagttcct tgaacagtat tcttgcaggg acagacggca ttattaccta
    10681 tgaaaaggat cgtctgggaa atattgtccc aggtactgag caagtttccc aacaaacggt
    10741 ggatggtaag gatgtttaca caaccatttc cagccccctc cagtccttta tggaaactca
    10801 gatggatgct tttcaagaga aggtaaaagg aaagtacatg acagcgactt tggtcagtgc
    10861 taaaacggga gagatactcg caacgacaca acgaccgacc tttgatgcag atacaaaaga
    10921 aggcattaca gaggactttg tttggcgtga tatcctttat caaagtaact atgagccagg
    10981 ttccactatg aaagtgatga tgttggctgc tgctattgat aataatacct tcccaagtgg
    11041 agaatacttc aatagcagtg aattaaaaat agcggatgtg acgattcgag attgggatgt
    11101 taatgatggt ttgactactg gtaggatgat gactttctta caaggtttcg ctctctccag
    11161 taatgttgga atgagtcttc ttgaacaaaa aatgggagat actacttggt tggattatct
    11221 aaaccgcttt aaatttggtg ttccgacccg tttcggtttg acggatgagt atgctgggca
    11281 acttcctgcg gataatatcg ttaacattgc gcaaagctca tttggacaag ggatttcagt
    11341 gacccagacg caaatgattc gtgcctttac agctattgct aatgatggtg ttatgctgga
    11401 gcctaaattt attagtgcca tttatgatcc aaatgatcaa actgctcgga aatctcaaaa
    11461 agaaattgtg ggaaatcctg tttctaaaga tgcagctagt ctaactcgga ctaacatgat
    11521 tttggtaggg acggatccgg tttatggaac catgtataac cacagcacag gcaagccaac
    11581 tgtaactgtt cctgggcaaa atgtagccct caagtctggt acggctcaga ttgctgacga
    11641 gaaaaatggt ggttatctag tcgggttaac caactatatt ttctcggctg tatcgatgaa
    11701 tccggctgaa aatcctgatt ttatcttgta tgtgacggtc caacaacctg aacattattc
    11761 aggtattcag ttgggagaat ttgccactcc tatcttggag cgggcttcag ctatgaaaga
    11821 ctctctcaat cttcaaacaa cagctaaagc tttggagcaa gtaagtcaac aaagtcctta
    11881 tcctatgcct agtgtcaagg atatttcacc tggtgattta gcagaagaat tgcgtcgcaa
    11941 tcttgtacaa cccatcgttg tgggaacagg gacgaagatt aaaaacagtt ctgctgaaga
    12001 agggaagaat cttgccccga accagcaagt cctgatctta tctgataaag cagaggaagt
    12061 tccagacatg tatggttgga caaaagcgac tgctgagacc tttgctaagt ggctcaatat
    12121 agaacttgca tttgaaggtt cgggctctac tgtgcagaag caggatgttc gtgctaatac
    12181 agctatcaag gacattaaaa aaattacatt aactttagga gactaatatg tttatttcca
    12241 tcagtgctgg aattgtgaca tttttactaa ctttagtagg aattccggcc tttatccaat
    12301 tttatagaaa ggcgcaaatt acaggccagc agatgcatga ggatgttaaa cagcaccagg
    12361 caaaagctgg gactcctaca atgggaggtt tggttttctt aattacttct gttttggttg
    12421 ctttcgtttt ttccctattt agtagccaat tcagcaataa tgtgggaatg attttgttta
    12481 tcttggtctt gtatggcttg gtcggatttt tagatgactt tctcaaggtc ttccgtaaga
    12541 tcaatgaggg gctaaatccc aagcagaaat tggctcttca gcttctaggt ggagttatct
    12601 tctatctttt ctatgagcgt gggggcgata tcctgtctgt atttggttat ccagttcatt
    12661 tgggattttt ctatattttc ttcgcccttt tctggctagt cggtttttca aatgcagtca
    12721 acttgacaga cggtattgac ggtttagcta gtatttcagt ggtgattagt ttgtcagctt
    12781 atggagttat tgcctatgtg caaggtcaga tggatatcct tctagtaatt cttgccgtga
    12841 ttggtggttt gctcggtttc ttcgtcttta accataagcc tgctaaagtc tttatgggag
    12901 atgtgggaag tttggcccta ggtggaatgt tggcagctat ttctatgacc ctccaccaag
    12961 aatggactct cttgattatc ggaattgttt atgtctttga aacaacttcg gtcatgatgc
    13021 aagtcagtta tttcaaactg acaggtggta aacgtatttt ccgtatgacg cctgtgcatc
    13081 accattttga gcttggggga ttgtctggta aaggaaatcc ttggagcgag tggaaggttg
    13141 acttcttctt ttggggagtt gggcttctag caagtctcct gaccctcgca attttgtatt
    13201 tgatgtaaga atggcacact gatgtttcag ggtgtttttg ctgtttaaaa aatattggtc
    13261 aattaaagtc aaaacccttg accttttctg actaatgtag tatattatga acgtaaggta
    13321 aatgaaagcc ttacgtgaac acttatgctt aatgaaaatc aagagcaaac taggaagcta
    13381 gctttaggca gctcaaaaca cggttttgag gttgtagata aggcggagct gatgtggttt
    13441 gaagagattt tcgaagagta taaaaaatat tggtcagtca aagtcaaaac ccttgacctt
    13501 ttctgactaa tgtagtatat tatgaacgta aggtaaatga aagccttact agaacgctta
    13561 tttaaagtaa aatagaaaga ggtggggtct atgtttaatc tagaaatctt cagaagtaaa
    13621 gatagtctac tcctgcttga aaaagaaaaa ccagaaatag tacctagagt agcgatttag
    13681 ctagtctaaa ttttttaaaa caaaggtcaa agatagtcaa tatcagtaat cataactaag
    13741 taaacaaaaa gaggtaaaga atatgaacaa caactttaat aattttaaca acatggatga
    13801 tttatttaac caattgatgg gtggtatgcg aggatacagt tctgaaaatc gtcgttactt
    13861 gattaatggc cgtgaagtga cacctgacga atttgctcac tatcgtgcaa ctggccaatt
    13921 gccaggaaat gcagaaactg atggacaaat gcaacaacag gcttcaggta tgaaacaaga
    13981 cggtgtcctt gctaaactgg gtcgtaattt gacagcagaa gcgcgtgagg gtaagttgga
    14041 tcctgttatc ggacgaaaca aggaaattca agaaacatct gaaattcttt cacgccgtac
    14101 caagaacaat cctgttttgg tcggagatgc aggcgttggt aagacagccg ttgtcgaagg
    14161 tttggcacaa gctattgtaa atggtgatgt tccagctgct attaagaata aggaaattat
    14221 ttccattgat atctcaggtc ttgaagctgg gactcaatac cgtggtagct ttgaagaaaa
    14281 cattcaaaac ctagtcaatg aagtgaaaga agcaggaaat attattctct tctttgatga
    14341 aattcaccaa attctcggtg ctggtagcac tggtggagat agtggttcta aagggcttgc
    14401 ggatattctc aagccagccc tctctcgtgg tgaattgaca gtgattgggg caacgactca
    14461 agatgaatac cgtaacacga ttttgaagaa tgctgctctt gctcgtcgtt tcaacgaagt
    14521 caaggtcaat gctccttcag cagaagatac ttttaaaatt cttcaaggaa ttcgtgacct
    14581 ctatcaacaa caccacaatg tcatcttgcc agacgaagtc ttgaaagcag cggtggatta
    14641 ttctgttcaa tacattcctc aacgtagctt gccagataaa gctattgacc ttgtcgatgt
    14701 aacggctgct catttggcgg ctcaacatcc agtaacagat gtgcatgctg ttgaacgtga
    14761 aatcgaagca gaaaaagaca agcaagaaaa agcagttgag gcagaagact ttgaagcagc
    14821 tctaaactat aaaacacgca ttgcagaatt ggaaaagaaa atcgaaaacc acacagaaga
    14881 catgaaagtg actgcaagtg tcaacgatgt ggctgaatct gtagaacgaa tgacgggtat
    14941 tccagtgtca caaatgggag cgtcagacat cgaacgtttg aaagatatgg ctcatcgatt
    15001 ggaacacaag gttattggcc aagataaggc agttgaagct gtagctcgtg ccatccgtcg
    15061 taaccgtgct ggttttgatg aaggcaatcg cccaatcggt agcttcctct ttgtagggcc
    15121 tactggggtt ggtaagacag aacttgctaa gcaattggcg ctcgatatgt tcggaaccaa
    15181 ggaagcgatt atccgtttgg atatgtctga atacagtgac cgaacagccg tttctaagct
    15241 aattggtacg acagcaggtt atgtgggtta tgatgacaat agcaatacct tgacagaacg
    15301 tgttcgtcgc aatccttact ctattattct tttggatgag attgaaaagg ctgatcctca
    15361 agtaatcact cttcttctcc aagtattgga tgacggtcgt ttgacagatg gtcaaggaaa
    15421 tacggtgaac ttcaagaaca ctgtcattat tgcgacatca aatgccggat ttggatatga
    15481 agctaatttg acggaagatg cagataaacc agaattgatg gatcgtttga aaccattctt
    15541 ccgcccagaa ttccttaacc gcttcaacgc agttatcgag ttctcacact tgacgaagga
    15601 agacctttct aagattgtag acttgatgtt ggctgaagtc aaccaaacct tggctaagaa
    15661 agacattgat ttggtagtca gtcaagcggc taaagactat atcacagaag aaggctatga
    15721 cgaagtcatg ggtgttcgtc ctctccgtcg cgtggttgaa caagaaattc gtgataaggt
    15781 gacagatttc cacttggatc atctagatgc caaacatctg gaagcagata tggaagatgg
    15841 tggtttagtt attcgtgaaa aagcctaaga tagaattttg aggataaaaa agaaggagcc
    15901 agctgaaaaa aaactggttc cttttgaggt acgacgggca tgtcgtacag taggttgaaa
    15961 ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgac tgtcctgatt
    16021 gatttgtcct gttcttattt cattttacta tatatctgag gtgtaagtcc tcggtgggta
    16081 cctgctaccg gtgctcccaa tagtgattcc caagcctgac tatcgtgagg tagcggatta
    16141 aaacggtctg gggatagacc gttttaagtc tgacgctgga aataagaatc gtcagaggaa
    16201 gggatagcga aatcgtggct ctacgaatag gaacgtgata ataaggcgta tatagcggat
    16261 aaggggcctc aaactctaaa gttcaaaaag gtagtcgtag cctatatgcg taaatcacga
    16321 gagcaactga atttgtctat tcgaggatgg ataaactatt tctcattggg aaatatgaaa
    16381 agtatagtca ccagcataga tgagcgtttg catactcgcc tacgagtgat tatctggaag
    16441 caatggaaga agaaatcgag acgattatgg ggattgctta agttaggggt tcctaaatgg
    16501 atagcagata aggtatctgg ctggggcgac cactatcaat tatagtaaaa tgaaataaga
    16561 atagaacaaa tcgatcagga tagtcaaatc gatttctaac aatgttttag aagcagaggt
    16621 gaactattct agtttcaatc cactatagta gctcagaagt cggtacttaa acgtgctata
    16681 tcaaaaccag tccttgaaaa acgtggattg gtttcgtgtt tggattatta ccttgaacga
    16741 catgcgttaa aagttagttg aaccgccgta tgccgaacgg cacgtacggt ggtgtgagag
    16801 gggctagaga ttatccccta ctcgatttta aatcacatga cgttcaaagg catcatctga
    16861 aatcccttgt tccaagatga gttttgccca ttctttagca gagaagaggc tgtggtcctt
    16921 gtagtttccg caagattcga tggttgtccc tgggacatct tcccaagtag tagtttcagc
    16981 gatttccttg agcgaatcct tgataacagc tgcgatttta gcactggtgt gacgtcccca
    17041 cataatcatg tggaagcctg tgcggcaacc aaatggtgaa cagtcaatca tgccgtcaat
    17101 gcgggtacgg atgagtttgg ctaagaggtg ctcgatagtg tgaaggccgg cagtagggat
    17161 agagtcttcg tttggttgca ccaagcgaat atcataattg gagatgatgt ctccttttgg
    17221 tcctgtttct tccccaatca agcgaacata gggtgctttg acaatggtgt ggtcaagttc
    17281 aaaactttcg acaataactt cttttgacat ggtaaatcct ttcagttttc ttctctcatt
    17341 atatcataaa gcttgctcct gagacagaga gaaaacctct ccgaggctgg agaggttgaa
    17401 atctttactt acgatataag cggtcgtatt ggtagtatgg gtcaaaggtt acgttgatac
    17461 ccagtttacg aaggacattc ttgtcttcat cagtcaagat gatggttgag tgggcttcgc
    17521 ttcctttgag gttgccgagt tcttccatag cgcgggcagc atcaggattt tctgtagctg
    17581 tgatagcaag tgcaatcagg atttcatttg aatgaaggcg tggattgcgg ctaccgagat
    17641 gatcgatttt aagaccttgg attggcttaa caacttcagg ctcgattagt tttacttctt
    17701 tagcgatgtc agctgatttt ttgatggcgt tgatcaaggc agcggctgta gggccaaaga
    17761 gttctgagtt tttaccagtg acaatgtcac catttggtaa ttcaagagct agggctggtc
    17821 cgccagtttc ttctgctttt tggcgcgcaa cgacagcaac cttacggtcg gcaggtgtga
    17881 tgccgaggtc gttcataagc aactcaattt tcttgacggc agcttcgcca actttttcag
    17941 ctttgaaatc aagaacggtt tgatagtaac ggcggatgat ttcttgttta gaagcttcga
    18001 cagcagcctc gtcatctgta atagcgaaac caaccatgtt gacacccata tctgtcggtg
    18061 aagcgtatgg tgattttccg agaatacgtt ccaacatgcg cttgagcact gggaagattt
    18121 cgatatcacg gttgtagttg acagtggttt ctccataggt ttgaagatgg aaggggtcaa
    18181 tcatgttgac atcatcaagg tcagctgtgg cagcttcata agccaagtta actggatgat
    18241 gaaggggaag attccaaaca gggaaggttt caaatttagc gtagccagac ttgatgccat
    18301 tgatttggtc gtggtacata ttggacatac acgttgccaa ttttccagaa ccgggtccag
    18361 gagcggttac gacaatcaag ttgcgactgg ttttgatgta gtcgtttttg cccatgcctt
    18421 ctggggaaat gatgtgatcc atatccgtcg gatatccttt gattggataa tgaagataag
    18481 aatcaattcc gtttttctca agttggttgc ggaaggcatc tgcagcgagt tggccagcgt
    18541 attgtgtaat gacaacggaa ccaacaaaaa tccctaattc attgaatttg tcaatcaaac
    18601 gaagaacttc ttggtcataa gaaatgccta agtcgccacg tgctttggaa tgttcaatgt
    18661 tgctagcatt aatggcaatc acaacctcaa cctgctcttt caattcttgc aagagcttga
    18721 ttttgttgtc aggttcataa ccaggaagga cacgagcagc gtggaaatct tctaacattt
    18781 taccgccaaa ctctaagtag agcttgccgt caaattggtt aatgcgctcc aaaatatggt
    18841 cgcgttgtaa attcaaatat tgttcagaac taaaagcttg ttttttcatt tttttacctc
    18901 tggactctat tataataaaa aattggaagt taggaaacta cggagctaaa aaagaaatta
    18961 aaaagattaa gcaaacgctt gcacaaaatt ttaaaaagtg ctatcataga ctatagatta
    19021 tgaaaataat gaggtaaaca gatgcaagaa aaatggtggc ataatgccgt agtctatcaa
    19081 gtctatccaa agagttttat ggatagtaat gaagatggaa ttggcgattt gccaggaatt
    19141 accagtaagt tggactatct agccaagcta ggaatcacag caatttggct ttctcccgtt
    19201 tatgacagcc ctatggatga taatggctat gatattgctg attatcaagc gattgcggct
    19261 atttttggaa ccatggagga catggatcag ctgattgcag aagctaagaa gcgtgatatc
    19321 cgtatcatca tggacttggt ggtcaatcat acctcggatg agcatgcctg gtttgtagag
    19381 gcctgtgaaa atcctaatag ccctgagcga gactactata tctggcggga tgagcctaac
    19441 gatttagatt ctatctttag tgggtctgct tgggaatacg atgaaaagtc aggtcaatac
    19501 tatctccact ttttcagcaa gaaacagccg gatctcaact gggaaaatga aaaacttcgc
    19561 cagaaaattt atgagatgat gaacttctgg attgataagg gtattggtgg tttccgtatg
    19621 gatgttattg acatgattgg caaaattcct gacgagaagg tagtcaataa tggtcctatg
    19681 ctccatccct atctcaagga aatgaatcag gcgacctttg gagataagga tctcttgaca
    19741 gtaggggaga cttggggagc aacgccagag attgccaaac tctactctga tccaaagggg
    19801 caagaattgt ctatggtctt ccagtttgaa catatcggtc ttcagtatca ggaaggtcag
    19861 cctaaatggc actatcaaaa agagctgaat atcgctaagt taaaagaaat cttcaacaaa
    19921 tggcagacag agttaggagt tgaggacggc tggaattccc tcttctggaa caaccatgac
    19981 ctccctcgta ttgtctcaat ctggggaaat gaccaagaat accgcgaaaa atctgccaaa
    20041 gcctttgcaa tcttacttca tctcatgaga ggaactcctt atatctacca aggtgaggag
    20101 attgggatga ccaactatcc gtttgaaaca ctggatcaag tagaagatat tgaatctctc
    20161 aactatgcgc gtgaggctct tgaaaaaggt gttccgattg aagaaatcat ggacagtatc
    20221 cgtgttattg gacgtgacaa tgcccgtacc cctatgcaat gggacgagag caaaaacgct
    20281 ggtttctcaa caggtcaacc ttggttggcg gttaatccaa attacgagat gatcaatgtc
    20341 caagaagcgc tggcaaatcc agattctatt ttctatacct atcagaaact ggtccaaatt
    20401 cgcaaggaga atagttggct aattcgagct gactttgaat tgcttgatac ggctgataag
    20461 gtctttgctt atatacgtaa ggatggcgac cgtcgcttcc tagttgtggc taacttgtcc
    20521 aatgaagagc aagacttgac agtagaagga aaagtcaaat ctgtcttgat tgaaaacacc
    20581 ctagctcaag aagtctttga aaaacaaatc ttagttccat gggatgcttt ctgtgtggaa
    20641 ttactataaa tattttttgc agaaaaattt aaaattgaaa tcatataaaa acaagggagg
    20701 actgtataaa aggcggaaat cctttgtttt ttataaccaa ggtttataaa ctttcattct
    20761 cgaaattcaa ttaactttac aaattcccac tattaaggag aaagaaaatg aacataaaga
    20821 agcgtgtcct tagtgcaggt ctgacttttg catctgcttt gcttttagct gcttgcgacc
    20881 aatcaggttc agatacaaaa acttactcat caacctttag tggaaatcca actacattta
    20941 actatctatt agactattac gctgataata tagtcaattg aaacaagaga aagacaaaag
    21001 agcctcgtaa aaggtattgc aacttggtaa tacctttttg aggtgctttt tgatatgagc
    21061 ccatgttttc tcaataggat tgtactcagg tgagtaggga ggaagaggta aaagtttatg
    21121 cccaaactct tcgcataaaa gttctagctt ccccattcta tggaatcttg cattatccat
    21181 aataataacc gatggtgtgg ttaatgttgg taagaaaaac ttctgaaacc aagcttcaaa
    21241 aaagtcgctc gtcatcgtct cttcgtaagt cattggaacg attaactcac catttgttag
    21301 acctgcaacc aaaga
//
LOCUS       DAVKWS010000034        20233 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKWS010000034 DAVKWS010000000
VERSION     DAVKWS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.034"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(144..293)
                     /locus_tag="VW852_001355"
                     /pseudo
     CDS             complement(144..293)
                     /locus_tag="VW852_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(447..2078)
                     /gene="rlmD"
                     /locus_tag="VW852_001356"
     CDS             complement(447..2078)
                     /gene="rlmD"
                     /locus_tag="VW852_001356"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0272495.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2152..3126)
                     /locus_tag="VW852_001357"
     CDS             complement(2152..3126)
                     /locus_tag="VW852_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV0272496.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3200..3814)
                     /gene="pmp23"
                     /locus_tag="VW852_001358"
     CDS             complement(3200..3814)
                     /gene="pmp23"
                     /locus_tag="VW852_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV0272497.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3814..4788)
                     /locus_tag="VW852_001359"
     CDS             complement(3814..4788)
                     /locus_tag="VW852_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV0272498.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4796..6052)
                     /locus_tag="VW852_001360"
     CDS             complement(4796..6052)
                     /locus_tag="VW852_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV0272499.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6114..6545)
                     /locus_tag="VW852_001361"
     CDS             complement(6114..6545)
                     /locus_tag="VW852_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272500.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGEGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6547..7149)
                     /locus_tag="VW852_001362"
     CDS             complement(6547..7149)
                     /locus_tag="VW852_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV0272501.1"
                     /translation="MMDRIRQELEKGGAVILPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7133..7972)
                     /gene="prmC"
                     /locus_tag="VW852_001363"
     CDS             complement(7133..7972)
                     /gene="prmC"
                     /locus_tag="VW852_001363"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV0272502.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNFN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(7972..9051)
                     /gene="prfA"
                     /locus_tag="VW852_001364"
     CDS             complement(7972..9051)
                     /gene="prfA"
                     /locus_tag="VW852_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV0272503.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9048..9638)
                     /locus_tag="VW852_001365"
     CDS             complement(9048..9638)
                     /locus_tag="VW852_001365"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV0272504.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9762..9944
                     /locus_tag="VW852_001366"
     CDS             9762..9944
                     /locus_tag="VW852_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV0272505.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAIVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10082..11455
                     /gene="mnmE"
                     /locus_tag="VW852_001367"
     CDS             10082..11455
                     /gene="mnmE"
                     /locus_tag="VW852_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV0272506.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTNSFAIAQKIFKGKDL
                     SQVASHTLNYGHIVDPLTGKVMDEVMIGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11502..12437)
                     /gene="dapA"
                     /locus_tag="VW852_001368"
     CDS             complement(11502..12437)
                     /gene="dapA"
                     /locus_tag="VW852_001368"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV0272507.1"
                     /translation="MSYQDLKKCKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12493..13569)
                     /locus_tag="VW852_001369"
     CDS             complement(12493..13569)
                     /locus_tag="VW852_001369"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV0272508.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13911..13983)
                     /locus_tag="VW852_001370"
     tRNA            complement(13911..13983)
                     /locus_tag="VW852_001370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13948..13950),aa:Thr,seq:ggt)
     gene            complement(14083..14592)
                     /locus_tag="VW852_001371"
     CDS             complement(14083..14592)
                     /locus_tag="VW852_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV0272509.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14590..14686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14715..15146
                     /locus_tag="VW852_001372"
     CDS             14715..15146
                     /locus_tag="VW852_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV0272510.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15228..15605
                     /gene="mscL"
                     /locus_tag="VW852_001373"
     CDS             15228..15605
                     /gene="mscL"
                     /locus_tag="VW852_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV0272511.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTDEKKPTELEVLQEIKALLEKK"
     gene            complement(15647..16741)
                     /gene="hemH"
                     /locus_tag="VW852_001374"
     CDS             complement(15647..16741)
                     /gene="hemH"
                     /locus_tag="VW852_001374"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV0272512.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYREEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            16896..18119
                     /gene="pepT"
                     /locus_tag="VW852_001375"
     CDS             16896..18119
                     /gene="pepT"
                     /locus_tag="VW852_001375"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV0272513.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKTVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18155..18784)
                     /locus_tag="VW852_001376"
     CDS             complement(18155..18784)
                     /locus_tag="VW852_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272514.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            18749..18970
                     /locus_tag="VW852_001377"
     CDS             18749..18970
                     /locus_tag="VW852_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272515.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19079..19648)
                     /locus_tag="VW852_001378"
     CDS             complement(19079..19648)
                     /locus_tag="VW852_001378"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0272516.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGDHYHYIPKSSLNNPPSHSNTEEVGSSSSSVLSNTSLH
                     VHHEEEDGHGFDANRIISEDSEGFVIPHGDHNHYIKVQTKGYEAALKNKIPSLQSNYQ
                     PGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(19692..20233)
                     /locus_tag="VW852_001379"
                     /pseudo
     CDS             complement(19692..20233)
                     /locus_tag="VW852_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5850 a         4248 c         3813 g         6322 t
ORIGIN      
        1 tgcttacaat atccattatt aaaatggata ctataagcag cacaccaact atttattttt
       61 tgagttttaa actttggtag aagtcttgaa ctccttatat ttacataact atttgcctct
      121 ataactatta tgacacgaaa ccaacaagca agttcaagca cctccatatc tttattcaaa
      181 tgaggactaa gatattcaca aacattatca taaacctctt tatctttttt cataaaagag
      241 gcatacaact tagcaaattt atcctaaaat tttttgttgc ctttatcaat catttcaacg
      301 ctcctttaaa tataaaaatg atcctatcaa tctttatatt gagcctattt tctaattgat
      361 accttgcaag aaaggaataa tagctgttcc tataccaagt aggaatgtaa taaatgcaga
      421 aagtcatata ctgtcattct tttctattaa atcattttga agtgtctcaa agcatccatg
      481 atggcttctt ctttttcagg tgtacactgt ggaataattt gtttatcctt tttagacttg
      541 ttgtaatgtt ctcgtaattc tattccacat ttctttttta tctgtgcaat atataatgtc
      601 gaaactttta attcaaattt attccaaaca tattctttga tttgagcata tgttgctttg
      661 ctctccgcac ttgtcaaatc catctcatcc agctcaattt caacacttat atgcttatcg
      721 acatcgagtt tggacaaaag tgctaccgtc tcgacgtgat gcgtttgagg aaatagatcc
      781 accggctgga ctttcttcaa ttcatatccc aactcttggt atagtttaat atcacgcgcc
      841 atggttgcga cattgcagga gatataggcg atgcgatcgg ctcctgtttg ggcgcttgct
      901 ttgataaagc tttctgtcaa gcccttgcgt ggaggatcaa ccaagataac ggttggttga
      961 atcccatctt taagccaatt cttcatagca ttttcagctg tgtcacagac atagtgggcg
     1021 tttgaaatat tgttcagctg agcatttttt ttactattct caaccgcttc tggaatcagt
     1081 tcaacaccgt agacttcttt gacatgcttg gcgactgata aaccaatggt tccaatacca
     1141 gaataggcat caataatcac gtcatctttt tttaactctg caaagtcaat ggctgtttga
     1201 tagagtttct ccgccatttc agtattgact tggtaaaagg ctgggccagc gatttggaag
     1261 tcatttccca acatctggtc cgtaatatag tcttgaccat aaagagtgcg ccactcctta
     1321 ccaaaaatcg cattggtatt ctggtcgttg atattttgca tgacagacac aatctctggg
     1381 aactgcttga taacttgttc aatcaattgg tcaacacgaa aaacttttgg acgagttgtc
     1441 accaaaacga ccatgatttg tcctgaatag tgaccacgac gcaccacaag attccgaatc
     1501 aatccagact gttccttttc gtcataaggt tttaaatcaa aacgacggag caggtctcga
     1561 agagctacta cgacttggtc aatgacagga tcctggataa agaaatcttc aaggggcatg
     1621 aggttgtgcg aattcttacg gaaaaatcct gtttccaaga caccattcac tcgacgaacg
     1681 ggcacctgcg ccttattgcg atacttgact ggatgttcca taccaagcgt ttcagcaact
     1741 tctacatctg caattccagc aatcttgtag agactgtcct tgacttgctt ggttttaaac
     1801 ttgagctgtt ctggataaga aaggtgtcct aaatccgcga ttcctgaacg caggtaagcc
     1861 aaatctagat cttgattacg gtgtggtgac tggacaaggt atttttcaac ttttccaaag
     1921 ccaatctttt tattgacctt gaggacacgc atgagaattt tttcactcgg taaagcattc
     1981 tctacaaaaa agaccaaacc atctaccttg gcaactcctg ccccttcatg ggtcaaatca
     2041 acaatttcaa cttctacaat atcatttttc tttaacattc ttttctcttt ctattggccg
     2101 acaaaaaaga cggcaacgtt acggttacca tccattatac catgaaattt cttaagaacc
     2161 aaaactcttg aagaagttga caagggcttg ccagagggag cttaagaagt taccaccgtc
     2221 ctctagcgcc ttatccgcat caaagttaag gttgatattt ttaaaactgt cgccagcttg
     2281 tgatacgatg ctttgtttaa ggtcatttag ggttttagtg aaatctgcat tgctgaggat
     2341 atcactcttt gagagattca aggcaaaatt gatgatgata ttgatctggt ttcctgttat
     2401 gacctgatca agtttgtaat tttttaaggt atcttcaaca atcttgcgga tatcttcttc
     2461 tgtcagattt cccttgcttt ctttagcttt ggcgagtcct gacttgatat cagctagggc
     2521 aacgtttaat ttattagcat catagcctga tttgtccttg ttttcagcat tgatatctga
     2581 caaagccttt agctcttctt gagccaaatc tttattggct tgcggcacct tggctccatt
     2641 agcttctagc gaatagtaga tcccagccaa agcactctca cctgtaactg gaatgggtgc
     2701 tgctacagtg attttggcgt gttccacacc caaagttacg gctgcatttc ggtacatatc
     2761 ctgagttacc ttagtgatgt tttcaggtgt ttcaatcttg acctcaagtg gcgatttgtc
     2821 acctagcttt tgaatcttgg ctgatgaata caactgtaag ctagagtcat tggccacatt
     2881 catgatttta gaataaacat caggtgtcat ggtcttgagt tctttggtat ctgttgaggc
     2941 attgtagccc agttttttaa gagtttgatt tttttggtct tcagataggg aagaacctag
     3001 gacatattca ggttggacat aggtttcatc gataactttt tgaacatctg ttgctgcatg
     3061 gacgctattc atagctgtta ctgcccacaa gatcgcagcg ctagtcagaa agagtttctt
     3121 tctcataggg aatttcctcc tttacttctt tagagtaata tatctatctt aaagaaaact
     3181 tataacaaaa acacctggtc tagccagatg ttgaaaagag agtgaaacat ttgatgatgt
     3241 aaaggttaag tcgtacctgt ctagaataat aatagtttcc tccatttaca tagagttcag
     3301 caccgtgaaa aatggaaatg gggtgaatat aactataagt ctttccagtc gtattaccaa
     3361 gcaagggggc aacagtctca cgagagtact gtttggctag agccaaggta ttttccttgc
     3421 cattttgggc gataaaatcg atataggcag gtccaaaatt ataggcttga acagctgtcc
     3481 agatatctac acccttcttc tgcgccagat agagattgcc tgtcagagtt tgaatgcctt
     3541 gccgaatgct agaggcatta tcattgatgg tgttggtgga accacttgca gactcactag
     3601 actgcataac atcgccttct tttccttttg tttcagtata aatcatagca agcacaagct
     3661 cttcgtttgc tggggtgtct ttttcactca atatttctcg caccatgggt tgataggtca
     3721 tgacttgttt gacatcttga tgaacgcggt aagctttata gccagcaaaa aggaagactg
     3781 ctagtacaag cactcttcga attcgtttaa acattattta ctttggatat cctcgatatt
     3841 tttgattaag atagagtagg ttccattttc gttttggata aactcaacag actcggcgtc
     3901 ttgatagacg ttattgggaa cgatgagctc aattccattt gataaggaga gtttttggtt
     3961 ttcaaatttc tttaattggc gactgacatc aatttcatca aattgaacag gttctggtac
     4021 ggcttctttg acttggtcaa taaagctcaa acgagccgtc agattgttgt caaaaaggtc
     4081 attagccaat ttctcaggtg acaattcatt gctttcttct aggttgttga aaatagctga
     4141 tttgaccttg gattgaaatt gaaaatcatc tgtgttaaaa gattcagcaa ttctctgggc
     4201 tgttttttcc agttccttga tagatttttt aggagaaatc ttaggagcga cagcaagaag
     4261 attatctgaa aaatagttca aaaaagtccc gttgtacttg attcgttttt caatcaggtg
     4321 atacttgcta ctctgaagat tgaccaccaa ggcctcatca gctcctgttc caaatccagg
     4381 caggttattc tgagttagct tgattggatt atcaacttct cctccgaggt gggtcaaggt
     4441 ctcccgcagg gcaattcgca agaaagcgaa atgttctaca ccttctttag aaaattgaac
     4501 aaaaatcaag tcattggtct tgagattttc agaaatgcta aactcctctt tccagagatt
     4561 agccagcgtt actgatgtct ccaacaaatc gtctgtaata tgattgaaga agggattttc
     4621 ttcttcgaaa atcccagtct tggcttcatc tgaatacaca tgttcaattt ttttacgcag
     4681 gtattcttcg atttttggag taatattgag aaacttatct gctaagaaca gttcggtatc
     4741 atccggactg aactggtgaa taatggcttt cttaatataa atgtccataa aagttttagt
     4801 cctcgtatag taggaaggca tctgtcaatt ctttgactgc acttctcact tcttctaata
     4861 cagcctcatt ttctgaattc ttaagggttt taatgatgag ttcagccact ttgcgacttt
     4921 cttcttcacc aaatccacgt gcagtaatgg ctgctgctcc gatacgaatc ccacttgtct
     4981 tgaatggtga caagctttcg taagggattg agtttttatt taaggtaata ttgacttcat
     5041 ccaacaagtt ttgagcaact ttgccgtttt ctacaacttt agtcacatca acaaggaaga
     5101 gatggttttc agttccacct gaaataatac ggaaatcagg gtcttgcaag aagacatctg
     5161 ccatagcctt gctgttctta attacattgg cagcatattc cttgaaggct ggatccaaaa
     5221 cttctttgaa ggaaactgcc ttagccgcca caacatgctc taaaggaccg ccctgaatac
     5281 ctgggaaaat agctgaattg atttttttag caagttcttc gtcattggtc aaaatcaaac
     5341 caccacgagg accacgaagg gttttgtggg tcgttgttgt tgtgatatga gcgtatggaa
     5401 ctgggcttgg atgaaggcca gccgcaacca aaccagcgat atgggccatg tccaccatga
     5461 gcttcgcacc gacagcatct gcgatttcac ggaattttga aaaatcgata atttgagaat
     5521 atgctgaagc accagctaca atcagttttg gttttacttc ttgggcttgt ttcaagatag
     5581 catcaaagtc taagagttcc gtttcaggat caacactata agaaacaaag ttgtaggttt
     5641 gaccagagaa gctaacagga gccccatgag tcaaatgacc acctgatgcc aaatccattc
     5701 ccataaccgt atcacctggc tcaatcaagg acatgtaagc cgcacagtta gcttggcttc
     5761 ctgaatgtgg ttggacattg gcaaatttag caccgaaaat ttcttttgcg cgttcaatag
     5821 caagagtctc tacaacgtct actacatcag ttccaccata ataacggcgt cctgggtaac
     5881 cctcggcata tttatttgtc aagatagacc cttgagctgc cataacagcc ttggaaacta
     5941 cgttttccga agcaattaac tcgatattat tttgttggcg ttcttcttct ttggcaatag
     6001 cattccagag atcagcatca tatgctttaa aatcatcttt gtcaaaaatc ataggtcttc
     6061 tcctttattg tgtgactagt ccattagttt gattttacaa taagaaaatc aaactaaaag
     6121 atgcgaataa accgtttctg cattttatca caagtatagc caactttttc ataaaatgca
     6181 tgagcaccca gacgatgatt ggcagaattt aagcggataa acccataacc acatcttttg
     6241 gcttcttgtt ccaacccttg tagtaaactt ttaccgatac cttcaccttg cgcttgaggt
     6301 gaaactgcta aagctaagat attaaatcct gctttggaat agagtgattc gtaaacttca
     6361 gcgtggacat atccaagtaa gacatgatta gctgcatcct catagccaag taggaaatga
     6421 tgggaatcct gagacagtct agctagttgg ctagccgttt cctctggact aaaagtataa
     6481 cccaaagcct cttggttgat gtcacatatc gctttcacat ctgtttcttg caaatctctt
     6541 agcatttcaa gcctcctcaa aagaaatctc tggcaaccga gcaagaatat cttctcgttt
     6601 aattgcgcct tggcgtaaga ttttcacctt gtctccagac aaatccacaa tagttgaatc
     6661 ctgtccagtt agaaaagcat cgtcttccag acccagaacc tcttggtcaa aatccttcag
     6721 aatttgttca aaggttacac cacttgcctg acctgagata ttggcagacg gcccaatcaa
     6781 gggacctgtc tctcgaatta aatccagtgt gatagggtga ctgggcatcc gaaatccaat
     6841 agttgcaagg tcagaattta cccaataggg aactcggtca ttggcttcga gaataatggt
     6901 caagggacct ggcaaaaagg tctctacaag tttttgtaga taagctggct gattcttaga
     6961 aaagtgcaag atatcctcta aagaggcgat attgagattg agtgccttat ctctaggacg
     7021 acgtttgagc tggtaaacat ggtcaactgc tttttcgtct aaggccttgg caaaaagacc
     7081 ataaaccgtc tctgtaggca gaatgacagc tccacccttt tccaactctt gtctaatcct
     7141 gtccatcatc aaccacgacc atcctatctt gaccaaattg gtccttgagt gttcgaactc
     7201 ttttttcagg aagatgtttc ctaaaaagtt cgggaacact ttgaccttgc ttgtatccaa
     7261 tttcaaggta aatcttacca ccatccttga gatagtcttt tgcatcttcc gcaattctac
     7321 agtaaatagc taggccatcc tcgtctgcaa agagagctag atgaggctcc gaatgcaaaa
     7381 cattcaagcc tacctctgac tcatcttcac gagagatata gggtggattg gatacaatta
     7441 tatcatattt ttcagaaatt tctgcaaaac aatcagattt tttaaaaaat atattaaaat
     7501 tttgatcgct agcattctct gatgcaagct ctaaggcctc ttgagaaaca tctgctgctg
     7561 tcactgacca atctggtcta ttttttgcta gagcgagagc aatagctcca ctacctgttc
     7621 caatatccag aactgagaga ttcgtctcaa gattttcagt tagaataagc tccactaact
     7681 cctctgtttc tggacgagga atcaaaaccc gttcatccac ctttaaatgc attccataaa
     7741 aatctgcctg tccaatgatg tactgagctg gcttgtgagc tgctagttgc tggtaaatat
     7801 cttctacaaa ttgtttttct tcctctgttg tcacctcctg ctggagggca aaaataaagt
     7861 ctgtaaaaga tagatttttc agactacgat agacaaaaga gaggctttcc gcttcctctc
     7921 cttgtcttat caactcttct tcaaaatttg aaaacaattg agctaatttc attatttgtt
     7981 taattcttct agtttttgtg tttggtcata aagcaccaag gcatccacaa cttcgtccaa
     8041 tttaccagac aaaatcgtat ctagtttttg gagagtcaag ccgatacggt ggtctgtgac
     8101 acggttttgt gggaagttat aagttcggat ccgttctgaa cggtcaccag taccgattgt
     8161 cgacttacgc tcagcgtctt gttcatcctg agcaatctga gcaaagtggt cagcgacgcg
     8221 tgcacggata atcttcatag ccttctcgcg gttcttctgc tgggtacgtt cttcctgcat
     8281 ctcaaccttg atattggttg gcaagtgaac gatacgcacg gcagtcgcaa ccttattgac
     8341 gttctgtcca ccagcaccag aggcgtgata gatgtcgaca cgaaggtctt ttggatcaat
     8401 gtcgtattca acctcttcaa cttctggcat aacaagaact gtcgctgtcg aagtatgaac
     8461 acggccttgg ctttctgtca caggaacacg ttgcacacgg tgggcacctg attcatactt
     8521 aagcttagag tatacagact gacctgaaac catagcaacc acttctttga aaccaccgac
     8581 accattcata gaagcctcca tgacttcaaa gcgccagcct tgggcttcag catacttttg
     8641 gtacatagtt agcaaatctc cagcgaaaag tgccgcttcg tctccaccag ctgctccacg
     8701 gatttcaagg atgatattct tgtcatcgtt tggatccttt ggaaggagca aaattttcag
     8761 tttttcttca tattcttctt tttcagcctt ggcatctttg agttcttgct tggccatttc
     8821 ttccaagtcc gcatctccac ctgattcctt aatcatctct tcggcatcga cgatattttg
     8881 aaggacttgt ttatactcac ggtaggctat tacggtgtca cgattggagg cttcttcttt
     8941 tgaaagctcc ataaaacgct tggtgtctga aacgacatca gggtcactca gcaattctcc
     9001 tagttcttca taacggtctt ctacagcttg tagttgatca tagatgttca tttttcttca
     9061 ttctccttat tgatttcagg ggcaaaataa tgtttacggc aaaccgagat ataggtttca
     9121 ttaccaccaa tctggatctg ttctccatcg taaacgggca gtccatcctg tgttcgcaac
     9181 accatggtcg cctttttctt gcaatactga cagatggtct tgatttcgtc aatcttgtcc
     9241 gctaaaagca agagatattt ggaaccttcg aacaattcat tgcgaaagtc atttttcaag
     9301 ccaaaagcca tgacgggtat gtctaactcg tctacaacac gagctaggtc gtaaacatgg
     9361 tggcgtttga gaaactgggc ttcatcgacc aaaacacagt aaggtttttc tggtaggtct
     9421 cggatatagc caaagatatc cgttgtttcc tcaatcgcaa gggcagggcg tttcatgcca
     9481 attcgactcg acacatagcc aacaccgtca cgcgtatcca gagccgaagt cataatgaca
     9541 acaccttttc cttgctcctc gtagttatag gccactttga gaatctcaat cgtcttacca
     9601 gagttcatgg tcccataacg ataatacaac tgtgccatgt ttcttgcttc acgtccattt
     9661 ctaaattttt gctacattct agtatatcat aattttctga agctttaaac ggcaaaatgt
     9721 ggtaaaatag aagaaatcaa aaactagtgg aggaagctat tatgccattt gtacgcatcg
     9781 atttatttga aggacgcacg ctcgagcaaa agaaagctct tgctaaggaa gtaacggaag
     9841 ctattgtccg taatactgga gccccccaat ctgctgtcca tgtcatcatc aacgacatgc
     9901 cagaaggaac ttacttccca caaggggaaa tgcgtaccaa ataagctagc ttaagcagaa
     9961 ttgcttaggc tttttcaatc tccaagtaac atccattgaa gaaatagcct aaatttgtta
    10021 caatttgaaa ggatacttgg aaaaatttcc aagaaaagag ctatttatta aaggaaacat
    10081 tatgattaca cgtgaatttg ataccatcgc tgctatctct actccactag gtgaaggggc
    10141 tattggtatt gtccgcctga gcggaacaaa cagttttgct attgcgcaaa agattttcaa
    10201 aggaaaagac ttgagtcagg ttgctagcca tacacttaac tacggtcaca tcgttgaccc
    10261 tctgactggt aaagtcatgg acgaggtcat gattggggct atgaaatctc caaaaacctt
    10321 cactcgtgaa gatattatcg agattaacac ccacggtggg attgcggtga ctaatgaaat
    10381 tctccagcta gctatccgtg aaggagctcg attggctgaa cctggtgaat ttactaaacg
    10441 tgccttccta aatggacgtg ttgacttgac tcaggctgag gcagtaatgg atatcatccg
    10501 tgctaagaca gataaagcca tgaacattgc ggtcaaacaa ctagatggtt ctctttctga
    10561 cctcattaac aatacccgtc aagaaatcct caatacactt gcccaagtcg aagttaatat
    10621 cgactatcct gagtatgatg atgttgagga agcgactact gctgttgtcc gcgagaaaac
    10681 tatggaattt gaacaattgc taaccaatct ccttaggaca gcccgtcgcg gtaaaatcct
    10741 tcgtgaagga atttccactg ccatcatcgg acgtcccaac gttgggaaat ctagcctcct
    10801 caacaatctc ttgcgtgagg acaaggctat cgttacagat attgctggta ccacacgtga
    10861 tgtcatcgaa gagtacgtca acatcaatgg tgtacctctc aaattgattg atacagccgg
    10921 tattcgtgaa acagatgaca tagtggaaca aatcggtgtt gagcgttcga aaaaagccct
    10981 caaggaagcc gacttggttc tactagtgct aaatgccagt gaaccactga ctgcgcaaga
    11041 cagacaactt cttgaaatta gccaagatac caatcgcatt attctactta ataaaaccga
    11101 cctgccagaa acgattgaaa cttcgaaact acctgaagac gttatccgta tttcagtcct
    11161 taaaaaccaa aacatcgaca agattgaaga gcgaatcaac aacctcttct ttgaaaatgc
    11221 tggcttggtc gagcaagatg ctacctactt gtcaaacgcc cgtcacattt ccctgattga
    11281 aaaagcagtt gaaagcctac aagccgttaa tcaaggtctt gagctgggga tgccagttga
    11341 tttgcttcaa gttgacttga ctcgtacttg ggaaatcctc ggagaaatca ctggggatgc
    11401 tgctccagat gaacttatta cccaactctt tagccaattc tgtttaggaa aataagaaaa
    11461 atccatgatc cttcattcgg tcatggattt tattgtcttt attagtaatc tggtcttaag
    11521 acccctgtta cagttgcctt agttgcttcg tagtcgccat ctacgacaac cttgataatg
    11581 cgtttggcat cttcttctgg tgctggaaca agaggtagac gagtgggtcc agcttcaaat
    11641 cccatatagt taagaattgc cttaactgga gcaggacttg gataagagaa gagagcatta
    11701 accttaggaa tgaatttacg ctgaattgct gcggctttct tcatatcgct ttctgcaatc
    11761 gcagtaaaca tctcgtgcat ttcatcccca tttgtatgag aggcaacaga aataacccca
    11821 tccgccccaa ggttcatggc atggaaagca tctccatcct cacctgtata aatcaagaac
    11881 tcttcaggct tgtgctcaat caagtaagcc atattagcca agctagtaca ttctttgaca
    11941 ccgataatat ttggatggtc agccaagcga agcatggttt ctggagtcaa ttcgacaact
    12001 acacgccctg gaatgttata gataataatt ggtaggtcag aagcatctgc aatagtctta
    12061 aagtgctgat acatcccttc ttgagaaggt ttgttgtagt aaggaacaat agcaagccca
    12121 gctgcgaaac caccaaattc cgctacttct ttgacaaact caatagagtc acgcgtatca
    12181 ttagtaccta cacccgcaat caaaggaacg cgtccattga caaccttttg tacagccgca
    12241 aacaactcca actcctcatc gtgggtcaaa gttggactct cagcagtcgt tcctgcgaga
    12301 agaattccat ccgtatgatg ggccaataaa tgctcaatca aggctggaat agcatcaaag
    12361 ttaatggaac catcctcatg gaagggggta ataaaggctg taatgatttt acattttttt
    12421 aaatcttgat aagacacgaa aacacctttc tatttcaaag aaggagttca tctgaactcc
    12481 taaacgatat gactatttta attcaaattt caattcggct gttggacgaa ccaatccacg
    12541 ttcatgaaga gtttcagcaa tctgaactga gttccaagca gcacctttga gaaggttatc
    12601 tgaaacaacc cacatgtgaa ttcctttttc tgcatccaag tctttacgga tacgaccaac
    12661 aaaggtatca cgcgaaccaa ctgcattgat agcttgagga tagatttgat gagctacatc
    12721 atcttcaaga acagcacctg ggaaggctgc gatagctgct tttacttctt cgattggagc
    12781 cacttctttt gtttcgatat aaacagactc agagtgagct gacaagactg gaatacgcac
    12841 acatgttgca gatactgcaa tgctatcatc ttccataatt ttcttagttt ccttggtcat
    12901 cttcatctct tcgtacgtgt aatcattatc agtgaaaaca tcaatttgtg gaagagcgtt
    12961 aaaggcgata ggataatgtt tcttgtcacc acctgaaggc aagatttccg catgcaaatc
    13021 acgtggtttc acaccatcat tcaagacttc acgaagttca cgttgtgtct caagaattgc
    13081 tcccatacca gcacctgaaa cggcttgata agttgaaaca atgatacggt ccaagcccca
    13141 tttttggcga accggctcaa gagccaccat catttgaatt gttgaacaat tagggcaggc
    13201 aatgattccg ttgtgagcat caagtgcatg agcattgacc tctggaacaa ccaaaggaac
    13261 atctggattt tgacggaaat aagatgtatt atctactact accacgccag cttttactgc
    13321 gtatggtgca tacttagctg atgtagaact acctgctgaa aagagagcaa tatcaactcc
    13381 ttcaaaagct gtttcagtcg tttcttcaat tgtaatatct tgatctttaa atttcaatga
    13441 cttacctgct gaacgtgcag aagcaaggta acgaatttta tcgattggaa gtgttgattc
    13501 ttccaacatt tttatcatct gagcaccgac agcacctgtc gcgccgacta cagcaactgt
    13561 atatcccata aataacctct ttcggaattt tctaaaaatt tctataatag aagtattata
    13621 ctactttttc catgaattgc aaagcttttt catcattttt tggaaaataa aaatcccagt
    13681 cgctagacta gggactaaga ggcatcttca tgtgatgagc aggttcacac aactcatcaa
    13741 ggtccgctcc tgcgttatga cctcctcatg ctcaatagta gtccgaagac tacctatcga
    13801 ctcatcgcat attctatatt actagagata cttgattttg tcaataagaa atttttcaga
    13861 tcgagtataa caaaaaactc tcgtattcaa gagtttccct atacatatca tgctaattgc
    13921 cgggattgaa ccggcgacct catccttacc atggatgcgc tctgccaact gagctaaatc
    13981 agcttactta agaagtatac tatattcaga aaaacttgtc aagtttcctt agaaattttc
    14041 cataggaaaa agccaggtaa aaactaccta gcttatcttc ttctatttac ttaaatcaat
    14101 tcgacgacca attttaccaa taataatcgc tccaataatc aatgaagcaa attcaccgat
    14161 tcctgttgta aaccaagtaa ggaagaatgg taattctgct acaatattta actctgcagc
    14221 gatagtaaac atggaaattg aaaagaggat tgaaaagaag aaatgatctt ttcgaatcaa
    14281 tccgttaaag aggtaatcct tgctatattt tgcaaaaagc caaacaccta aactgaggaa
    14341 tactaaggtt gaaccaccac caacaaagac atctagcagt ccgaagctaa agaaattagc
    14401 aatcatacaa ccgatggtaa ctccgatgat gtacttagga ttgtaaaaag ccataaagtt
    14461 catcatttct gaaatacgaa actgataagc accatagctg atggcattta ggggcggtgt
    14521 gacagtcaaa acgacgtaga tagcagcgac gattgcaata tctgcaacat cacgaatagt
    14581 taattttttc atcttttctc ctttggcggt tatccgcgtg taatatgctt ggtgaaagaa
    14641 gctaagcacc aagggttgat tcattcaacc ttactagtat agcacaatag cctgctttat
    14701 gctatactta aagcatgaaa aaacctattc aaaaattttt taacaatgaa atcttagcct
    14761 atctcttttt tggtatagtg actactcttg ttgctatact atcacgatta gtcatttatc
    14821 agctaagtca tcaggaactc cttgctacgg ccttggcaaa tattatcggt atcctatttg
    14881 cctttatcac aaatgatacg attgtattta agcaaaaaaa gaaaaataga cttatccgtt
    14941 tagtgaaatt ttctcttgcc cgcctttcta cttttctgtt agacttgttt ttaacttttc
    15001 tctttgtaac ccaatttcct cacatcatag ggcaattcgt aactgaaaac attgataaga
    15061 taaatgctat tgaaacaata attgcacaaa tattcattat tatcatcaat tatatcttga
    15121 gcaaggtcta tatttttaga aaataatgct ccatttatga gccttttaat tgccttctat
    15181 aatgatttag gatacaatga aacacgaaac attgaaagga aaacagtatg ttaaaaaatc
    15241 taaaatcgtt cttgcttcga ggaaatgtta ttgaccttgc tgtcggtgtt gtaattgcct
    15301 ctgcttttgg tgctatcgtt acttcacttg taaacgacat tatcactcct cttattttaa
    15361 atcccgcttt gaaagctgct aaagttgaac gtatcgctca actttcttgg catggagtcg
    15421 gctatggtaa cttcttaagt gctattatca attttatctt tgtgggtacc gccctcttct
    15481 ttattatcaa gggcattgaa aaagcacaga agctgactgg cataaaggaa gaaaaaactg
    15541 acgaaaaaaa accaaccgaa ttggaagtcc ttcaagaaat aaaagctctc cttgagaaaa
    15601 aataatcgat taaaaggatc tagcgcaaag ctaaatcctt ttttctttac tctttgggta
    15661 acttgcctgc tttttcaagc atttttttga taagataagg catcttaaca ttctcccgcc
    15721 cttttttctc aatcagtttt ttcacaaatt ctggcatttg taaatcttca gattcagcta
    15781 aaatgttttt cgtctcgtct gaaggaacta actcatcaaa ggtctcttct tctgggagaa
    15841 attccttaaa ctcttgattt tcattgactc taactgtatt gaccaaagca tcaatcaaac
    15901 gagaatctgt atttggcatt ggtggacgat ggtagtttac cccaaattcc tggcataagt
    15961 cataacattc tacatcattg tcaaacaaga cctcaatatg ctcactgatg aaactaatag
    16021 gtacaaaaat ataatggtct ggatgttctg tctgttctct gagatactcc aaaacatctg
    16081 gtttaatcca tggaatacca atatcactct cactctgcca agtgttggta tattgttctg
    16141 aactcaagcc cagtttttca gcgactagct tgctattttc gaaaatttgg tctatataag
    16201 gatcaccaaa gtccaaggca aaaatgggca cactatgggc tgaaaagatg actttaaagc
    16261 tgtcttgctt tacttcttct tttaaaatct tagcaatttc atctgcccaa tagtttaaga
    16321 gcgcttcttc ccgataccag tccttaatga ccaaaaattg aatttgtttg ctttctaaaa
    16381 atttttcata gcccatgaca gagtagaaag aataatgggg ctccaaaatc aagcaaatac
    16441 actgttcaat tccgtctgct tccatttgac cgatcacatc tggaataaag ggactggaaa
    16501 acttattagc aaagtagaca ctgtattcat ttcccagcct agcttctacc aaggcaacct
    16561 cttgacgggt aattttttgc agaggcgtgc ctccaattcg tacataatta tcatagagag
    16621 tctgaatctc gtggtcttgg ggtctcaccc cacgacgaat atttgtgaaa aaatcagcta
    16681 caccttcaaa ggtaatctct tctggtgaac cgaaggtcat cattaaaatt gcttttttca
    16741 tgactgcttc cttgtgatgt ttttatcaag tttattttat cattaattca tcgataagta
    16801 aacgctaaca tagggacatt ctcaatcttc tgtcttttgt ttttctcttc tttctatgat
    16861 acaatggaaa aaataaattc aaaaggagtt tttttatgac ttatccaaat ctcttggacc
    16921 gcttcttaac ctacgttaag gtcaacacgc gctctgatga acactctact actactccaa
    16981 gtacacagag tcaggttgac ttcgcaacaa atgtcctaat tcctgaaatg aaacgtgttg
    17041 gactgcaaaa tgtttactat ctaccgaatg gttttgctat tggaaccttg ccagccaacg
    17101 atccgtcttt aacacgtaag attggtttta tatcgcacat ggatactgct gattttaatg
    17161 ctgaaggagt caatccacag gtaattgaaa actacgatgg tggtgtgatt gaactaggga
    17221 attctggttt caaactcgat ccagccgact tcaagagtct tgaaaaatat ccaggacaaa
    17281 cgctcatcac aacagatgga acaagcttac taggtgctga tgacaagtca ggaattgctg
    17341 aaattatgac agccattgaa tatctaactg ctcatcctga aattaagcac tgtgagattc
    17401 gtgttggttt tggtccagat gaagaaatcg gtgttggtgc caataaattt gatgcagaag
    17461 attttgatgt ggattttgcc tacactgttg atggtggtcc actaggtgaa cttcagtacg
    17521 agactttctc agccgctggt gctgaattgc atttccaagg ccgtaatgtc caccctggta
    17581 ctgccaaagg gcagatggtc aatgcccttc agctagcaat tgattttcat aatcaacttc
    17641 cagaaaatga ccgacctgag ttaactgaag gttaccaagg tttttaccat ctaatggatg
    17701 tgacaggtag tgttgaggag gtgcgtgcaa gctacatcat tcgtgatttt gaaaaagatg
    17761 cctttgaagc gcgtaaagca tccatgcaat ctatcgctga taagatgaat gaagaacttg
    17821 ggagcgaccg tgtcactctc aacttgacag accagtacta caatatgaaa gaagtcattg
    17881 aaaaagatat gactccaatt accattgcta aaaccgttat ggaagatcta ggtatcacgc
    17941 ctattatcga accaatccgg ggtggaacag acggctctaa gatttccttt atgggaatcc
    18001 caactccgaa tatctttgca ggtggcgaaa atatgcacgg acgttttgaa tacgttagcc
    18061 ttcagactat ggaacgtgca gttgatacca tcattggcat cgtagcttat aaaggctaaa
    18121 aagacgaggt ggctcagcta cttcgccttt ctttttattc tactggtttt tcttgatttc
    18181 cagtagttgt agaagattct gttgttttat tttctgaagt tgattcagca ggtttagaat
    18241 ctcttgtatt gcttggtttg ttttcgtcgc tagcagtttc aatgttagat tctgcagttg
    18301 cgtttggttg gttctcagca ctggtgttat caccatttgc ttcagcattt cttgctggac
    18361 ttgtttcttc acttgcgcta gcttttgact ggatttgatg attcaaaact agaatagctt
    18421 ttgtcgattc aagtaaagct gttttgtctt tactcttagc agaaagttga tctaataatg
    18481 catccacctt atcaaagtcc gcatcagatc cattattact ttctaaataa gagtgaagcg
    18541 acatgagaat atcgtagagt ttttgataga gtacaagtgt ctgaggatct tgctcagcat
    18601 tttccttttc ttgttgaagg gcgctagcga tacgagtcaa aacatctttt acctgactgt
    18661 ttacttcatc caagtctgca tcagccttgt ttgtggcagc ttttagattt tctacttctt
    18721 ctgccaagga ttgtctgatt ccttcttcat ggatttgttc caagagttga tttgccttgt
    18781 tcaaaagact ttctacttct tccttgctat ctgtcgcaga ttattggttg ctatctacca
    18841 tgtactccta aaacaggaga gttataatcc aagattacaa ggccttacag aaataagaaa
    18901 tccagataag acaatgttcg tccaagacgc tattcgcttc gcacagcagc acggattcaa
    18961 tatgctttaa ttttaaagtt taggtgtcaa gacctctttt tagtgtgccc aaaatttaga
    19021 gaagtaatca atcaactaac ttttattttt ttcaaacttt cagtaaactg acctaaagct
    19081 aactcaatct gtctttgtag atgcttctgc tatcagctag aagttgatct acttttgcca
    19141 agactgcctt ctcatcaaaa gttccaggtt gatagttgga ttgcagggat ggaatcttgt
    19201 ttttcaaagc cgcttcatat cccttagttt gaaccttgat gtagtgattg tggtcgccat
    19261 gaggaattac aaaaccttct gaatcttcac ttataattcg attggcatca aaaccatgac
    19321 catcttcttc ctcatggtgg acatgtagtg acgtattact taatacagaa ctagaagaac
    19381 ttcctacctc ttccgtgtta gagtgtgatg ggggattgtt aagagatgac ttaggaatat
    19441 agtgatagtg atccccatgt cttactatat aagcatcacc tgtatctctg acaatatcat
    19501 tagggttaaa gacatatgtg gctgctaatt cacctgccga caagtcactc tcaggaatga
    19561 aatgatagtg accaccatgt ggtactatag taggttgaaa tagaatatga gcaaattgat
    19621 aaggggattt taaagtaatt tctaacaatg atttagaaac tatgatgtgc tattctaaat
    19681 tcaactcact atatataacc atcatcggta gtataacgtc cctgtaattt tgctacagat
    19741 acttctgcac tagctccttt atcgtcttta ccatgttctt gtttttggcg attgatttca
    19801 tcttttgttc gtacattttc tgcatgagct tgatctttaa ggtaaacata atactttcca
    19861 tctaccttaa taatatatcc tcccttaacc taactgacga tatcttgatc tttcggctga
    19921 tagttggggg ctttcattaa tagctcttca ctaaagagcg catcaaaagg aactttacca
    19981 ttatagtagt gataatgatc gccatgagaa gttacataac cttgacctgt aatcttaata
    20041 acaatttgtt ttgcttgaat tccttctttt tgactaacct agtctggagt caaattttca
    20101 gtcttcttag tgtctttatt actgtttaca tatgaaacac gatttttatc tgtattggcc
    20161 tgttagctat gttggttcag agcataaaca cacagactta aggaaaggat aacaacagat
    20221 ccagctgcta tat
//
LOCUS       DAVKWS010000035        18424 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKWS010000035 DAVKWS010000000
VERSION     DAVKWS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.035"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(82..891)
                     /locus_tag="VW852_001380"
     CDS             complement(82..891)
                     /locus_tag="VW852_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0272517.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHHRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(893..1696)
                     /locus_tag="VW852_001381"
     CDS             complement(893..1696)
                     /locus_tag="VW852_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0272518.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1706..3193)
                     /gene="tacF"
                     /locus_tag="VW852_001382"
     CDS             complement(1706..3193)
                     /gene="tacF"
                     /locus_tag="VW852_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV0272519.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVGIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3529..4236
                     /locus_tag="VW852_001383"
     CDS             3529..4236
                     /locus_tag="VW852_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV0272520.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4241..5263
                     /locus_tag="VW852_001384"
     CDS             4241..5263
                     /locus_tag="VW852_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0272521.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5280..6149
                     /gene="licA"
                     /locus_tag="VW852_001385"
     CDS             5280..6149
                     /gene="licA"
                     /locus_tag="VW852_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV0272522.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNVVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            6139..7017
                     /locus_tag="VW852_001386"
     CDS             6139..7017
                     /locus_tag="VW852_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV0272523.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7029..7718
                     /locus_tag="VW852_001387"
     CDS             7029..7718
                     /locus_tag="VW852_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV0272524.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7804..8652
                     /gene="dprA"
                     /locus_tag="VW852_001388"
     CDS             7804..8652
                     /gene="dprA"
                     /locus_tag="VW852_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV0272525.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9536..10579
                     /locus_tag="VW852_001389"
     CDS             9536..10579
                     /locus_tag="VW852_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV0272526.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            10746..12833
                     /gene="topA"
                     /locus_tag="VW852_001390"
     CDS             10746..12833
                     /gene="topA"
                     /locus_tag="VW852_001390"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV0272527.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12939..13298
                     /locus_tag="VW852_001391"
     CDS             12939..13298
                     /locus_tag="VW852_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV0272528.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13391..14023
                     /locus_tag="VW852_001392"
     CDS             13391..14023
                     /locus_tag="VW852_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV0272529.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGYSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            14067..14723
                     /locus_tag="VW852_001393"
     CDS             14067..14723
                     /locus_tag="VW852_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV0272530.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14742..15836
                     /locus_tag="VW852_001394"
     CDS             14742..15836
                     /locus_tag="VW852_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV0272531.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NVIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15848..16885
                     /gene="leuB"
                     /locus_tag="VW852_001395"
                     /pseudo
     CDS             15848..16885
                     /gene="leuB"
                     /locus_tag="VW852_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            16882..17151
                     /locus_tag="VW852_001396"
     CDS             16882..17151
                     /locus_tag="VW852_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV0272532.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17155..17514
                     /gene="leuD"
                     /locus_tag="VW852_001397"
     CDS             17155..17514
                     /gene="leuD"
                     /locus_tag="VW852_001397"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV0272533.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNDLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17733..17876
                     /locus_tag="VW852_001398"
     CDS             17733..17876
                     /locus_tag="VW852_001398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272534.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            17976..18149
                     /locus_tag="VW852_001399"
     CDS             17976..18149
                     /locus_tag="VW852_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272535.1"
                     /translation="MTDVRGTSCFVIKFGKAGEQLAAKLWEEGKMVYASSASMIKRLK
                     LAMRARCNGVYGR"
     gene            18139..18300
                     /locus_tag="VW852_001400"
     CDS             18139..18300
                     /locus_tag="VW852_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272536.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
BASE COUNT         5756 a         3167 c         3903 g         5598 t
ORIGIN      
        1 gtgggaatat gtatttcatt ttctactaaa attatgtttt tgaataacct ctatcttagt
       61 agtctgtata atccccctca atcagttttt gcgataagct ttaatgctat gactatacca
      121 ctcttgcatt tcttttgatg gtggtgtcat ataatcgcca tacatctggg ttaaaaattg
      181 gtcatatttt ttgggaacag gcaacatacg gccctcaaac tcagttaaaa tcagttcttt
      241 aaaggtatca actgggaaga tttctttcat cccttcctta ccgatcccaa ctcctccttc
      301 atattgagga gtgttggtta cagcattttt gactagttga tcaattttct tgtaaaagta
      361 gcgaggattg acaaatcgga gagcgtacca gctacataat ctaagaaaat cttttagttt
      421 gctatcaccg tgaactgctc gtgatttttt gatataagct agttgacgaa gggccacata
      481 cttatagctc ttgtcgacaa tgctcaagtc tgtaaatcga tcaattggga agacatcgat
      541 gaaaaggctg gtatcatgac gcttgtactt aacatggtct tctataacag tagaagtgtc
      601 caaaatcgat gcgaaattat ggaagtacca agaagatgta tcgtaggaaa gaactttgta
      661 gcgatggtga ttttcttctt caataatctt cagtaaacgc tcataatcct cacgataaag
      721 ggaaatatca atatcatcat cccaaggaat catacctttg tggcggatgg ctccaagcat
      781 ggttccataa ctgagaaaat aaggaatatc atgtttctta caagtctcat caatatagtc
      841 cagcagggct agttgaattt ctttaatttc ttttttttct aaatattgca tcctaatcct
      901 ccaatttata agcgtgaaat tcatgactgt agaagcgttt ttcttctggt ggtagggtca
      961 tataatctcc ataaaattgt gtcaaaatag tatcaaattt ttcaggtgca ggaaggctta
     1021 aattctcaaa gggtaaatcg attgttttat caaaggtacc acttgggaag acttcctttt
     1081 ccttaaattt tgaaggaata aaagccatat actgcccatt ttcacgacta tatttttgaa
     1141 tttctttctc gattttattt gcaaaataac gaggagaaac cggtcgaagg agcaaccaaa
     1201 aggctgttcg tatccaatct tttaaaaggc tatccttata gacaatattt ttatgtttac
     1261 tgaaagacag cagtttgaag ctttccagtt tataacaagt atcaatgacc ttaggatcat
     1321 caaagcgatc catagggaaa atatcgataa agataccaga ctcataggtt tttgtatttc
     1381 gagtatcaat aattttagtc gtactgtcgg ttatcttgat aaagttgtta aagtagttct
     1441 tatcagtttc taaggatagg agcttatact tgcttttttc cttttgaaaa atgttaataa
     1501 atcgttggta gtcttctcta ggcatggaca gatcaatatc gtcgtcccaa gggataaagc
     1561 cctcatgtcg aaccgcccca atcagagtac cgtagttaat aatatagttg atattgtgct
     1621 ttttacagag agtatcaata taatccaaaa tttctaattc aatttgtttg gcatcttcaa
     1681 cggttagttg tttcatttta aactcctatg attttttgaa tttatttttt aaggctagga
     1741 catggtttaa aaattcatag aaaatgctat cttttgtgaa gacaagtaga ctaatataag
     1801 agatagctga taacaagaca atcaaaccag tattaatcaa aaatggcaaa ttaatgacca
     1861 tatccacagg atacacgaaa ttaatcagga aataaattcc cacaaaggaa agtgaaaaga
     1921 gcgagtatcg aacagtatag ctaaagatat gtcccaagtg gatgagttgt tttctatgga
     1981 tgaaaatgat atagaaaaca agtagagaag tctctgataa catagttgtc agtaagtagt
     2041 attcaggagc cacgatatgg ttgaaaaaga ggagactatt caagcccaaa ttgagtagcc
     2101 cagcaaagac tgtatagact gtgatacgtt tttcatagcc attggtaaag agaatttggg
     2161 aaccaagaat ggtatctaag gccagaataa tcgtacgaaa agcgaagaga gaggtcaaga
     2221 tgccgcctcc gatatatttt tcactaccgt aaagtaggat ggcatttggt cctaaaacca
     2281 tgagtccaaa actcagtgga atgataaaga agttaaagat tcgactacct ctattaacca
     2341 gagaaacata ggcttctttg tctcctttcc ccagatagta actgagacga ggcacactca
     2401 ctccaattgc acctgttaca accccagcta taacggtcac aattcgctga gctatggtat
     2461 agtaactaac gttgacatca atccctgttt taacgaggaa gaggcgatct aaaaaagtga
     2521 agagcatatt ggcattggca aagactaaca tggctgtcag agggagaaag agtggtttaa
     2581 aatcacttag gtgaatctta acaagtttga tgtctctttt aatccaaaaa taactaatca
     2641 ggtagttaat cagcgtcgat aaactcatca caagtgtata gacaacaata tcgtgttcat
     2701 ttttaacaaa taagaaaata gagaccagca tcaggatacg gatgaaggca gttttgtaaa
     2761 agagaaaact gtaattttcc agagcttcat tgacccattc gattgaaaaa atctgggcaa
     2821 tgagttgaat ccccataaca aggtagacct ttttgacgat tggattatca gtaaagaaga
     2881 gaggataggc taggatatag acagcagtgg tcaaaatcgt acaagcgatg cacaaataaa
     2941 aaagactaga aaaggtcctg ttaagatctt ttttgttatc cttgacatta ctgatagccc
     3001 ttaaaccgta gttatagaca ccataagttg caaagggcaa gaaaaatgac aaaatagtgt
     3061 cgactgagtt gaagtaacca tagtcagttc ggtccaagac acgcgcgaca taggttccag
     3121 ttaggatggg aaaaataata ttcaagacac gaattcccat gtaagataga gcatttaatt
     3181 ttatactttt cattcaattt acctcgtttt tcattatatc ataaagttag ctaataagaa
     3241 atgaagggca gtaagtcaag taatcacttt gaagtttcaa atcttaagtt ttaagttttc
     3301 tttaaggaaa gtatattatt ctgaaggact ctaaaatttc gcagccattt attagtaatt
     3361 gctacagaat tcctagtcat tcctagaaat ggactagttt ctttgaataa tagaactgca
     3421 taattctcct attctagaag gggaggacca gtatttcttt tatgatagga ctagattgtg
     3481 gtataataga gagaataagt ttttttagta agacaaagga gaaaatagat gatttatgca
     3541 ggaattcttg ccggtggaac tggcacacgc atggggatca gtaacttgcc aaaacaattt
     3601 ttagagctag gtgatcgacc tattttgatt catacaattg aaaaatttgt cttggagcca
     3661 agtattgaaa aaattgtagt tggtgttcat ggagactggg tttctcatgc agaagatctt
     3721 gtagataaat atcttcctct ttataaggaa cgtatcatca ttacaaaggg tggtgctgac
     3781 cgcaatacaa gtattaagaa aatcattgaa gccattgatg cttatcgtcc gcttactcca
     3841 gaggatatcg ttgttaccca cgattctgtt cgtccattta ttacacttcg catgattcag
     3901 gacaatatcc aacttgccca aaatcatgac gcagtggaca cagtggtaga agcggttgat
     3961 actatcgttg aaagtaccaa tggtcaattt attacagata ttccaaatcg tgctcacctt
     4021 tatcaaggac aaacacctca aacattccgt tgcaaggact tcatggacct ttatggatct
     4081 ctttctgatg aagagaagga aatcttgaca gatgcatgta aaatctttgt gatcaaagga
     4141 aaagatgtgg ccttggccaa aggtgaatac tcaaatctga agattacaac cgtaacagat
     4201 ttgaagattg caaaaagtat gattgagaaa gactagtaaa atgattaatc aaatttatca
     4261 actaactaag cctaagttta tcaatgtcaa atatcaggaa gaggctattg accaagagaa
     4321 tcatatcctt atccgtccca actacatggc tgtctgtcat gcggatcagc gttactatca
     4381 gggaaaacgt gatcccaaga ttttgaataa aaagcttcca atggcaatga ttcacgagtc
     4441 atgtggaacc gtcatttctg acccgaccgg aacctacgag gttggtcaaa aagttgtcat
     4501 gattcccaat cagtctccta tgcagagtga tgaagaattc tatgaaaact acatgacagg
     4561 gacccatttc ttgtctagtg gatttgatgg ctttatgaga gagtttgttt ctctccctaa
     4621 agatcgtgtg gtggcttatg atgctattga agatacggtt gcagccatta cagagtttgt
     4681 cagtgtgggc atgcacgcta tgaatcgtct attgactctt gctcatagca agcgggagcg
     4741 gatcgccgtt attggagatg gaagtttagc ttttgtggtt gccaatatta tcaactatac
     4801 tttgccagaa tcagagattg tggttattgg tcgtcattgg gaaaagttgg aactcttctc
     4861 atttgccaaa gaatgctata ttacggataa tattcctgaa gatttggcct ttgaccatgc
     4921 ttttgaatgt tgtggtggtg atggtactgg accagctatt aatgacttga ttcgctacat
     4981 tcgtcctcag ggaacaattc tcatgatggg agttagcgaa tataaagtca atctcaatac
     5041 tcgcgatgcc ttagaaaagg gcttgatttt ggttgggtca tctcgttctg gtcgcattga
     5101 ttttgaaaat gctatccaaa tgatggaagt caagaaattt gccaatcgtc ttaaaaatat
     5161 cctttatcta gaagaacctg taagagaaat taaagatatt catcgtgtct ttgcaaccga
     5221 tttaaacaca gcctttaaaa cagtgtttaa gtgggaagta taagtactgg aggttaattg
     5281 tggagaaaat cattaaagaa aaaatttctt ccttacttag tcaagaagag gaagtcctca
     5341 gtgttgaaca actgggtgga atgaccaatc aaaactattt ggccaaaaca acaaataagc
     5401 aatacattgt taaattcttt ggtaaaggga cagaaaagct tatcaatcga caagatgaaa
     5461 agtacaatct tgaactacta aaggatttag gcttagatgt aaaaaattat ctttttgata
     5521 ttgaagctgg tatcaaagta aatgagtata tcgaatctgc gattacgctt gattcaacgt
     5581 caatcaagac caagttcgac aaaattgctc caatattaca aactattcat acatctgcta
     5641 aggaattaag aggagaattt gctccttttg aagaaatcaa aaaatacgaa tccttgattg
     5701 aagaacaaat tccttatgcc aactatgaat ctgttagaaa tgtagtcttc tccttagaga
     5761 aaagactggc tgacttaggt gttgacagaa aatcttgtca tatcgatttg gtgcctgaaa
     5821 actttatcga atcacctcaa ggacgacttt atttgattga ctgggaatat tcatcaatga
     5881 atgatccaat gtgggatttg gctgccctct ttttagagtc tgaattcact tcccaagagg
     5941 aagaaacttt cttatctcac tatgagagtg accaaacacc ggtttctcat gaaaagattg
     6001 ctatttataa aattttacaa gatactattt ggagtctatg gactgtatat aaggaagagc
     6061 aaggtgaaga ttttggtgac tatggtgtga atcgttacca aagagctatt aaaggtttgg
     6121 cttcttatgg aggttcagat gaaaagtaaa aacggagttc cttttggcct tctctcaggt
     6181 attttctggg gcttgggtct aacggttagt gcttatatct tttcgatttt tacagatttg
     6241 tcaccctttg tggtggctgc agctcatgat tttttgagca tctttatctt actagctttt
     6301 ctcttggtaa aagaagggaa agttcgcctc tcaattttct taaatattcg caatgtcagt
     6361 gttatcatcg gagccttgct agcaggccct atcggtatgc aggccaatct ttatgcagtt
     6421 aagtatatcg gaagttcttt agcttcatct gtatcggcta tttaccctgc gatttcagtt
     6481 ctattggctt tcttcttttt gaagcacaag atttcgaaaa atactgtatt tggaattgtc
     6541 ttgattattg gagggattat tgcccagacc tataaagttg aacaggttaa ttccttctac
     6601 attgggattc tctgtgcttt ggtttgtgct attgcatggg gaagtgagag tgttcttagc
     6661 tcctttgcta tggaaagtga actgagtgaa atcgaagccc tcttaatccg tcaagtgact
     6721 tcgttcttgt cctatcttgt gattgtgctc ttctctcatc agtcatttac tgcagtagcc
     6781 aatggacaat tgctaggtct catgattgtt tttgcagcct ttgatatgat ttcctacttg
     6841 gcttattata tcgctatcaa tcgcttgcaa ccagccaagg ctacaggctt gaacgtgagc
     6901 tatgtagtat ggacggtctt gtttgcagtt gttttcttgg gtgcaccgct agatatgctg
     6961 acaattatga cgtcacttgt cgtcattgct ggagtttata ttattattaa agaataaagg
     7021 agattcgtgt gaaagccatc atcttagcag cgggattggg aactcgcttg cgtcctatga
     7081 ctgaaaatac ccctaaagcc ttggttcagg ttaatcaaaa acctttgatt gagtaccaaa
     7141 ttgagtttct caaagaaaaa ggaatcaatg acatcatcat cattgttggt tatcttaaag
     7201 aacaattcga ttacttgaaa gagaaatacg gtgttcgtct cgttttcaat gataaatacg
     7261 ctgactacaa taacttttac tctctctatc ttgtaaaaga agaattggcc aacagctatg
     7321 ttattgatgc tgacaattat ctctttaaaa atatgttccg caatgatttg acacgttcga
     7381 cttattttag tgtttatcgt gaagattgta ccaacgaatg gttcttggtc tatggagatg
     7441 actacaaggt tcaagacatt attgttgata gcaaggcagg tcgcatcctt agtggtgtat
     7501 ccttctggga tgctccaact gcagaaaaga ttgtcagctt tatcgacaag gcttatgcaa
     7561 gtggtgaatt tgttgatctc tactgggaca atatggttaa ggataatatc aaagagctag
     7621 atgtctatgt tgaagaatta gaaggcaata gcatttatga gatcgatagt gtccaagact
     7681 atcgtaaatt agaagaaatt cttaaaaacg aaaattaaag attccaacat ctgacaaaat
     7741 agtcggatgt tttttgattt tttacgaata gatagatgag tagaaaaaga aatggagtta
     7801 tttatgaaaa tcacaaacta tgaaatctat aagttaaaaa aatcaggttt gaccaatcaa
     7861 cagattttga aagtgctaga atacggtgaa aatgttgatc aggagctttt gttgggtgat
     7921 attgcagata tctcaggttg ccgtaatcca gccgttttta tggaacgtta ttttcagata
     7981 gacgatgcgc atttgtcgaa agagtttcaa aaatttccat ctttctctat tttagatgac
     8041 tgttatcctt gggatttgag tgaaatatat gatgcgcctg tacttttatt ttacaaggga
     8101 aatcttgacc tcctgaaatt cccgaaggta gcggtcgtgg gcagtcgtgc ttgtagcaaa
     8161 cagggagcta aatcagttga aaaagtcatt caaggcttgg aaaatgaact ggttattgtc
     8221 agtggtctgg ccaagggcat tgacacagca gctcatatgg cagctcttca gaatggcgga
     8281 aaaaccattg cagtgattgg aacaggactg gatgtgtttt atcctaaagc caataaacgc
     8341 ttgcaagact acatcggcaa tgaccatctg gttctaagtg aatatggacc tggcgaacaa
     8401 cctctgaaat ttcattttcc tgcccgtaat cgcatcattg ctggactttg tcgtggtgtg
     8461 attgtagcag aggctaagat gcgttcaggt agtctcatta cgtgtgagcg agcaatggaa
     8521 gaaggacgcg atgtctttgc tattcctggt agcattttag atggactatc agacggttgc
     8581 catcatttga ttcaagaagg agcaaaattg gtcaccagtg ggcaagatgt tcttgcggaa
     8641 tttgaatttt aaaaatgacc taagctagaa ttctaagaaa aaatcaattt taagagaaaa
     8701 tgaacccaac atttccataa taaaacgcat attagcaagt ttttaacact tgataatatg
     8761 cgttttttct aagtggatta gtagagtaga ggatttttct catataatac tcttcgaaaa
     8821 tctcttcaaa ctacgtcagc ttccatctgc aacctcaaaa cagtattttg agctgacttc
     8881 gtcagtctta tctacaacct caaagcagtg ctttgagcaa cctgtggcta gcttcctagt
     8941 ttgcgctttg attttcattg agtataaggg aaagtatagt gaattgaaat aagatgtgaa
     9001 caactctatc aggaaagtca aattaattta tagaaatatt ttagcagcca aggtgcactg
     9061 ttatagattc aatacactat aatttagtgt aattgagaaa ggagaaatga ttgtgattga
     9121 tgttggctag gttatgttca atgattccta ccgtctcaaa tcttgtcagt aaggaaaaat
     9181 aaattcttca aaagtagaga ttacaaggct tgtttaagaa agaattcaaa gaccttgaca
     9241 aataaaaata aaatggttat tataagaaat ggtctgaaat agatgatgat acttttcgaa
     9301 aatcttttta cgtcagctca gctttgcctt gctgtgtttt gagcaagcta cggttagctt
     9361 ccgagtttga ttttcattta ctagaaatga aactgatgag agatatcagt agacatttga
     9421 gtcaggatat tatggaaaat gataaaaaga gctcgtgaga ttggcatatc agactactaa
     9481 agtattgagt ttgttaggat tttagcgact agttagctgg gaaaggaaga tatttgtgac
     9541 aaataataaa ctgtattcgt tgatagaatt tagaaataaa atatatgaag aattagaact
     9601 ttccagaagt gatttagcga ttttactatg tgccatgctt atcgcctcta tcggattaaa
     9661 tatggattcg actcccgtga ttattggagc catgttaatc tctcctttga tgacacctat
     9721 tctgggagtg gggctctctc tagctatatt tgattttaaa ttgttaagaa aatcttttaa
     9781 aatattagct attcaaattc ttgccagttt aatagcttca acactttatt tttatctttc
     9841 tcccatttcg tatgctagtt cggaaattgt tgctagaacc tctccgacta tttgggatgt
     9901 tctcattgct tttgtaggag ggatagcagg tattattggt gctaggaaaa aagagaccaa
     9961 taatattgtt cctggtgttg ctattgcaac cgccttgatg cctcctcttt gtacagtagg
    10021 ttatgctatt gcttctgcta atctaaaatt tatcataggc tcctcttacc tattcctcat
    10081 caattgtagc tttattgtca ttgcgactta tataggtgtt aggttgatga tggttaagaa
    10141 acattatttt aaagataatg aagaagactc taaaatgcgt aggattttgc ttctagttgc
    10201 tgttttgctg atgattccga gtttcatctc tgcaacgact ttagtgagag aaacgttgaa
    10261 aaaagagtcc cttaagaaat ttatatcaga gcagtttcag gggcataata ttttgaaaaa
    10321 aacctattct aaaaagactc ataccctaaa gctaaccatt tcaggaaatt atttgacaga
    10381 agaagaactc gatatgattt caagtaagag aggtgactat ggtttaagtg atgtttctgt
    10441 tcaagtttca caattgtctg attcagaaca acttagtaag gaagaactgg tggagtattt
    10501 cttccagtat atcaaggata aggaagcaaa agaaaaggaa aaagctaata agttttatac
    10561 agagtctgag gagcaataat ttcttgagaa tagctggttt ctctcgtgag tcttctatgt
    10621 atatcaaagg aagactgagg ttttaagtat aaaacttttc ttctattata gtagctaagt
    10681 cttgacaggg ttcaaaaaat ggtttacact ttataaagtt tattactttg aaaaggtgtg
    10741 atactgtggc tacggcaaca aaaaagaaaa aatcaacagt taaaaaaaat ctagtcatcg
    10801 tggagtcgcc tgctaaggcc aagacgattg aaaaatatct aggcagaaac tacaaggttt
    10861 tagccagtgt cgggcatatc cgtgatttga agaaatccag tatgtccgtc gatattgaaa
    10921 ataattatga accgcaatat attaatatcc gaggaaaagg ccctcttatc aatgacttga
    10981 aaaaagaagc taaaaaagct aataaagttt ttctcgcgag tgacccggac cgtgaaggag
    11041 aagcgatttc ttggcatttg gcccatattc tcaacttgga tgaaaatgat gccaaccgtg
    11101 tggtcttcaa tgaaatcacc aaggatgcag tcaaaaatgc ttttaaagaa cctcgtaaga
    11161 tcgatatgga cttggtcgat gcccaacaag ctcgtcggat cttggaccgc ttggtagggt
    11221 attcgatttc gcctattttg tggaagaagg tcaagaaggg cttgtcagca ggtcgcgttc
    11281 agtccattgc ccttaaactc atcattgacc gtgaaaatga aatcaatgcc ttccagccag
    11341 aagaatactg gacagttgat gctgtcttta aaaagggaac caaacaattt catgcttcct
    11401 tctatggagt agatggtaaa aagatgaaac tgaccagcaa tgacgaagtc aaggaagtct
    11461 tgtctcgtct gacgagtaaa gacttttcag tagatcaggt ggataagaaa gagcgcaagc
    11521 gcaatgctcc tttaccctat accacttcat ctatgcagat ggatgctgcc aataaaatca
    11581 atttccgtac tcgaaaaacc atgatggttg cccaacagct ctatgaagga attaatatcg
    11641 gttctggtgt tcaaggtttg attacctata tgcgtaccga ttcgactcgt atcagtcctg
    11701 tagcgcaaaa tgaggcggca agcttcatta cggatcgttt tggtagtaag tattctaagc
    11761 acggtagcaa ggtcaaaaac gcatcaggtg ctcaggatgc ccatgaggct attcgtccgt
    11821 caagtgtctt taatacacca gaaagcatcg ctaagtatct ggacaaggat cagctcaagc
    11881 tatataccct tatctggaat cgttttgtgg ctagccagat gacagcggcc gtttttgata
    11941 ccatggctgt taaattgtct caaaaagggg ttcaatttgc tgccaatggt agtcaggtta
    12001 agtttgatgg ttatcttgcc atttataatg attctgacaa gaataagatg ttaccggaca
    12061 tggttgttgg agatgtggtc aaacaggtca atagcaaacc agagcaacat ttcacccaac
    12121 cgcctgcccg ttattctgaa gcaacactga ttaaaacctt agaggaaaat ggggttggac
    12181 gtccatcaac ctacgcgcca accattgaaa ccattcagaa acgttattat gttcgcctgg
    12241 cagccaaacg ttttgaaccg acagagttgg gagaaattgt caataagctc atcgttgaat
    12301 atttcccaga tatcgtaaac gtgaccttca cagctgaaat ggaaggtaaa ctggatgatg
    12361 tcgaagttgg aaaagagcag tggcgacggg tcattgatgc cttttacaaa ccattctcta
    12421 aagaagttgc caaggctgaa gaagaaatgg aaaaaatcca gattaaggat gaaccagctg
    12481 gatttgactg tgaagtgtgt ggcagtccaa tggtcattaa acttggtcgt tttggtaaat
    12541 tctacgcttg tagcaatttc ccagattgcc gtcataccca agcaatcgtg aaagagattg
    12601 gtgttgagtg tccaagctgt catcagggac aaattattga gcgaaaaacc aagcgtaatc
    12661 gcctattcta tggttgcaat cgctatccag aatgtgaatt tacctcttgg gacaagcctg
    12721 ttggtcgtga ctgtccaaaa tgtggcaact tcctcatgga gaaaaaagtc cgtggtggtg
    12781 gcaagcaggt tgtttgtagc aaaggcgact acgaggaaga aaagattaaa taagaggagg
    12841 ggcccgaaag taaatttcag gctccttttg gttagaactt gacaaaatac atcattttat
    12901 gcgaaactag aagaagatta ttttaactag gagaagttat gcgtcttatc tatctgataa
    12961 ttggtttttt atcactggcc ttggctattg ttggggttgt tttacccttg ttgccgacaa
    13021 cgcctttcct tttgttgtct attgcttgtt tctccagaag ttccaagcgt ttcgaagact
    13081 ggctttatca taccaagctc tatcaaacat atgtagctga ttttcgcgag actaagtcta
    13141 ttacgcgtga acgaaagaaa aaaatcatcg tctctatcta cgtcttgatg ggaatttcta
    13201 tttattttgc acctctttta ccagtcaaaa tcggtctggg tgctttgacc atctttatca
    13261 cttattatct tttcaaggtc attccagaca aagaatagtt aaaacagtag ttatttgcct
    13321 tgataaaatt gaaagcatat tcataataat atgatataat aaatttgaag taatattcaa
    13381 ggagaatcaa atgatttacg aattttgtgc tgaaaatgtg actttacttg aaaaagcgat
    13441 gcaggctgga gctcgtcgga ttgaactctg tgataatcta gcagttggtg ggacaacacc
    13501 cagctatgga gtgactaagg cagcggttga actggcagct aactacgata caaccatcat
    13561 gaccatgatt cggccacgtg gtggtgactt tgtctataat gacctagaaa ttgctatcat
    13621 gctagaagac attcgtttga ctgctcaggc tggaagtcaa ggggttgtat ttggagcttt
    13681 aactgctgat aaaaagttgg ataagcctaa tctggaaaag ttaattgctg catcaaaagg
    13741 aatggaaatt gtctttcaca tggcctttga tgaactaagt gatgaagatc aaccggaagc
    13801 tattgactgg ctcagtcaag ccggtgtcac tcgtatccta actcgtgctg gtgtgtctgg
    13861 ctactcctta gaaaaacgtt ttgttcacta tcacagaatt ttggagtacg ctaaaggtaa
    13921 aattgaaatt ctaccaggtg gggggattga ccttgaaaac cgtcaaacct ttatcgacca
    13981 ggtgggggta acacaattgc atggtactaa ggttgttttt taaaaaatag aaaggaactg
    14041 ctagctttgg gtagtagttt tcacttatgt ttgaaatttt taaatcctat caatttaatc
    14101 aagaaaaggc tcatgattat ggttttatag aaaatagcga agtctggaca tatagttgcc
    14161 agattttgca aggtgacttt gtcatgactg tgtccatcac tgctgataat gtgaactttc
    14221 aagtctttga ccaagagact ggtgacctct atcctcacgt ttatatggaa agcatgaggg
    14281 gaagttttgt cggaaatgtc cgtgaggctt gtctggagat tctttaccag attcggaagg
    14341 cttgttttga tgtgcaagat tttatctgtc atcagactaa gcgtatcatg actcaagttc
    14401 aggaaaagta tgggaaccag ttggagtatc tgtgggaaaa atcgcctgat acagctgtat
    14461 tgcgccatga aggcaatcaa aagtggtatg ccgtcttgat gaaaatctct tggaataagc
    14521 tggaaaaggg cagagaagga caagtggaag cagtcaacct caagcatgac caagtagcta
    14581 atttgctttc acaaaagggg atttatccag ccttccatat gagcaagcgc tactggatta
    14641 gtgtgtccct tgatgatact ttatcagatg aagaagtact ggaattgata gaaaaaagtt
    14701 ggaacttaac ctctaaaaaa tgaaatattt taataatttt catgaacttt caattagcta
    14761 aatattcttt actgaagaaa ttttcagaaa atataggatt taccacacca gaggaatatg
    14821 gtgccatctt caaatacctg attgagaatg tcaagacgga tcgtcagatc atctattcgc
    14881 ctcactgtca tgatgacctc ggaatggcag tggcaaatag ccttgctgct gtcaagaatg
    14941 gtgcaggacg tgttgaaggg actatcaatg gtattaggga gcgagctgaa aatgctgctt
    15001 tggaagaaat tgcagtggct ctcaatattc gccaagatta ctaccaagta gaaaccagta
    15061 ttgtcctaaa tgagaccatc aatacgtcag aaatggtttc tcgcttctct ggtattccag
    15121 ttcctaaaaa caaagccgtc gttggtggca ataccttctc ccacgaatct ggtattcacc
    15181 aagatggagt ccttaaaaat cctctcactt atgagatcat cacacctgaa ttggttggtg
    15241 ttaagattct gcttggaaaa ttatctggtc gccatgcttt tgttgagaaa ctgagagaat
    15301 tggccctaga ttttacagaa gaggatatca aaccactctt tgctaagttc aaggcactgg
    15361 tcgataagaa gcaagaaatc acagatgcag atattcgagc tttggtagct ggaaccatgg
    15421 ttgaaaatcc agaaggcttc cactttgatg atttacaact tcaaactcat gcagataatg
    15481 acattgaagc gctcgttagc ctagccaata tggatggtga gaaagtcgaa tttaatgcga
    15541 cagggcaagg ttccgttgaa gcaatcttta atgttatcga taagttcttt aaccaatctg
    15601 ttcgtttggt gtcctacact atcgatgcgg taacagatgg aatcgatacc caggatcggg
    15661 ttttggtcac tgttgaaaac agagatacag aaaccatctt taatgcagca gggcttgatt
    15721 ttgatgtgtt gaaggcttct gctattgtct atataaacgc taataccttt gttcaaaaag
    15781 agaatgcagg tgagatggga cgcagtgttt cttaccacga tatgcctagt gtgtaaagga
    15841 gaaggctatg gcaaagaaaa tagtagctct agcaggagac ggaattggcc cagaaatcat
    15901 ggaggttggt ttagaagttc tggaggctct agctgaaaaa acaggttttg actatgagat
    15961 tgacagacga ccgttcggag gtgcagatat tgatgcagca tgacctccct tacctgatga
    16021 aacccttaag gcaagtaggg aagcagatgc tatcctacta gtagctatcg gtagtcctca
    16081 gtatgatgga gcagtggttc gccctgaaca aggcctgatg gctctccgta aggaactcaa
    16141 tctttacgct aatattcgtc ctgtaaaaat ctttgacagt ctcaagcatt tgtcactact
    16201 caaactggaa cgaattgctg gtgtagactt tgtcgtggtg cgtgaattga caggcgggat
    16261 ttactttgga gatcatattc ttgaagagcg caatgcgcgt gatatcaacg actatagcta
    16321 tgaggaagtg gagcggatta ttcgcaaagc ctttgaaatt gcaagaaatc gcagaaaaat
    16381 cgttactagt atcgataagc aaaatgttct agcgacctca aaactctggc ggaaagtagc
    16441 tgaggaagtc gcacaggatt tcccagatgt aaccttggaa catcagctgg tagactcagc
    16501 tgctatgctt atgattacca atcctgctaa gtttgatgtt attgtaacgg agaatctttt
    16561 tggagatatt ttatctgatg aatcaagcgt cttatctggt acacttgggg ttatgccatc
    16621 agccagtcat tctgaaaatg gaccaagtct ctatgaacct attcacagtt cagcacctga
    16681 tattgcaggt caaggaattg ccaatcctat ttccatgatt ttatcagttt ccatgatgtt
    16741 gagagatagt ttcggacgtt atgaggatgc agagcgtatc aaacgtgctg ttgagacaag
    16801 tctggcggca ggaattttaa cgagagatat aggaggtcag gcttcaacaa aggaaatgac
    16861 ggaagctatt attgcaaggt tatgaagtta gacgaaaaaa ttactctagt ccttttgatt
    16921 tggaatgtca tcattttctt gatttatggt attgacaaat ctaaggcaag gagaagagtt
    16981 tggcgcatcc ctgagaaaat cttacttatt ttagccttta cttttggtgg ttttggggcc
    17041 tggttagctg gaatcacttt tcaccacaag actcgaaaat ggtactttaa aacagtttgg
    17101 tttcttggga tggtgaccac actagtagcc ttatatttta tttggaggta atggatggca
    17161 gggtcttcga gggaacacgc tgcttgggct ctagcggact atggttttaa ggtcgtgatt
    17221 gcaggatctt tcggtgacat tcattacaat aatgaactca ataatgacct gttgccaatc
    17281 gttcagccta gagaggttag agagaaacta gcccagctaa aaccaaccga ccaggtaact
    17341 gtggacttgg aacaacaaaa aatcatctca ccagttgaag aattcacctt cgagatagat
    17401 agcgagtgga aacataaact cctaaatagt ttggatgata tcggtattac cttgcagtat
    17461 gaagagttga ttgctgctta tgaaaaacaa cgaccagcct actggcagga ttagaaaaaa
    17521 tagaaaagga aatatagtaa actgaaataa gatgtaaaca aatgaattag agcttaacat
    17581 ccatttccag caatttttta gaaactacag tggactattc tggattcaac acattataaa
    17641 attatgacaa aacacattca caagaaggct acgacatttt aaaaggtgag ggcggatgta
    17701 tcgtttgccc tactaaagtt ggttacatta tcatgaccag tgataaggca ggacttgagc
    17761 gtaagttcgc agccaaagaa cgtaagcgta acaaaccagg tgttgttctc tgcggtagca
    17821 tggatgaact ttgcgcttta gcgcaactca acccagaaat tgaagcattc tactaaaaac
    17881 attgggatga agatattctt cttggttgta tccttccttg gaaaccagaa gcctttgaaa
    17941 aactcaaagc atacggggat ggccgtgaag aacttatgac tgatgtacgt ggtactagct
    18001 gttttgttat caagtttgga aaagcaggtg aacaattggc tgccaagctt tgggaagaag
    18061 gtaaaatggt ctacgcctca tctgcttcaa tgataaaacg attgaaactc gctatgagag
    18121 caaggtgtaa tggtgtctat ggtcgataag gacggcaaac tcatcccaga acaaggagga
    18181 gcacgttcaa cttcaccagc accagttgtg atccgtaaag ggcttgacat tgataaaatc
    18241 atgatgcacc tgtcagatac ttttaactca tgggactacc gtcaggttga gtattattaa
    18301 gataaaaaaa gaagtctagt gttatgagat attaaagctc ctaacactgg actttttgtt
    18361 tagaatttct tttctttttc tataggatat ggtattctat gtagaaaata tatgttaata
    18421 agta
//
LOCUS       DAVKWS010000036        18242 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKWS010000036 DAVKWS010000000
VERSION     DAVKWS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.036"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..383
                     /locus_tag="VW852_001401"
     CDS             <1..383
                     /locus_tag="VW852_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0272537.1"
                     /translation="EREIVTPTYREALITREKSFPTGLDMEFLGKDLPNVAIPHTDIV
                     HNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGN
                     GHLEDLSKISEPEKLYAYIAEATA"
     gene            421..723
                     /locus_tag="VW852_001402"
     CDS             421..723
                     /locus_tag="VW852_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0272538.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            731..1533
                     /locus_tag="VW852_001403"
                     /pseudo
     CDS             731..1533
                     /locus_tag="VW852_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1582..2046
                     /locus_tag="VW852_001404"
     CDS             1582..2046
                     /locus_tag="VW852_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV0272539.1"
                     /translation="MGGHPGFNCPLFDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYALFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2203..2628
                     /gene="lacA"
                     /locus_tag="VW852_001405"
     CDS             2203..2628
                     /gene="lacA"
                     /locus_tag="VW852_001405"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV0272540.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2659..3174
                     /gene="lacB"
                     /locus_tag="VW852_001406"
     CDS             2659..3174
                     /gene="lacB"
                     /locus_tag="VW852_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV0272541.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3185..4114
                     /locus_tag="VW852_001407"
     CDS             3185..4114
                     /locus_tag="VW852_001407"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV0272542.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIKGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            4116..5096
                     /gene="lacD"
                     /locus_tag="VW852_001408"
     CDS             4116..5096
                     /gene="lacD"
                     /locus_tag="VW852_001408"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV0272543.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            5330..6166
                     /locus_tag="VW852_001409"
     CDS             5330..6166
                     /locus_tag="VW852_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0272544.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6202..6519
                     /locus_tag="VW852_001410"
     CDS             6202..6519
                     /locus_tag="VW852_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0272545.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6519..8210
                     /locus_tag="VW852_001411"
     CDS             6519..8210
                     /locus_tag="VW852_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0272546.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKIGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            8425..9831
                     /gene="lacG"
                     /locus_tag="VW852_001412"
     CDS             8425..9831
                     /gene="lacG"
                     /locus_tag="VW852_001412"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0272547.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTTLEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9921..10133
                     /locus_tag="VW852_001413"
     CDS             9921..10133
                     /locus_tag="VW852_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV0272548.1"
                     /translation="MNFEKYSQIPCLTSEELKNLGKWYVSTGKEWICHSDDDLEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(10412..11173)
                     /locus_tag="VW852_001414"
     CDS             complement(10412..11173)
                     /locus_tag="VW852_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0272549.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(11355..12317)
                     /gene="nrdF"
                     /locus_tag="VW852_001415"
     CDS             complement(11355..12317)
                     /gene="nrdF"
                     /locus_tag="VW852_001415"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV0272550.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(12505..14664)
                     /gene="nrdE"
                     /locus_tag="VW852_001416"
     CDS             complement(12505..14664)
                     /gene="nrdE"
                     /locus_tag="VW852_001416"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV0272551.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(14745..14963)
                     /gene="nrdH"
                     /locus_tag="VW852_001417"
     CDS             complement(14745..14963)
                     /gene="nrdH"
                     /locus_tag="VW852_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV0272552.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            15314..15577
                     /locus_tag="VW852_001418"
     CDS             15314..15577
                     /locus_tag="VW852_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV0272553.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            15583..17316
                     /gene="ptsP"
                     /locus_tag="VW852_001419"
     CDS             15583..17316
                     /gene="ptsP"
                     /locus_tag="VW852_001419"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV0272554.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFNEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHSEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            17569..>18242
                     /gene="phtA"
                     /locus_tag="VW852_001420"
     CDS             17569..>18242
                     /gene="phtA"
                     /locus_tag="VW852_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV0272555.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASE"
BASE COUNT         5714 a         3297 c         3770 g         5461 t
ORIGIN      
        1 aagaacgaga aattgtcact ccaacttatc gtgaagcctt gatcacgcgt gaaaagtcat
       61 ttccaactgg tttagatatg gaatttctag gaaaggactt gccaaatgta gcgattcctc
      121 atacagatat tgttcataat ctagctgaga aagtggtggt tgttcgatta gaaaaaccag
      181 taacttttca caatatgata gctcctgata aggaagtaga agtatcccta ctcttcttta
      241 ttattaacaa ttcaagttca agtcaaacaa atattctggc tcagttgatg gactttttca
      301 caggaaatgg acatcttgaa gatttatcaa aaatttccga accagaaaaa ctttatgctt
      361 acattgctga agcaaccgct taatcttgtc tattaaaaaa taaaatcgga ggaaatccaa
      421 atgattaaaa ttcttgctgc ctgcggtgca ggtgttaact caagtcacca aattaaaagt
      481 gctctagaag aagaactttc aaaccgtggt tacgatgttc actgtgatgc agttatggtg
      541 aaagatgtaa acgaagacct tatgaaaggt tacgatatct ttacaccaat cgctgcaaca
      601 gaccttggtt ttgaaccagg tatcccagtt atcgaagctg ggccaatctt attccgtatc
      661 ccagcaatga gcgttccagt atttgacaat attagacttc ctgcgaaaca aaacatggta
      721 tagtagttct atgaatgatg aagcaagtaa acaactaact gatgcacgat ttaagcgtct
      781 tgttggtgtt cagcgcacga cttttgaaga gatgttagct gtattaaaaa cagcttatca
      841 acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc ttcttatggc
      901 cactcttcaa tatgtgcgag aatatcgaac ttatgaagaa attgcggctg attttggtat
      961 ccacgaaagc aacttactcc gtcggagcta atgggttgaa gtaactcttg ttcaaagtgg
     1021 tgttacgatt tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgcgacgga
     1081 agtacaaatc aatcgcccta aaaaagaatt agcgaatgat tctggtaaaa agaaatgcca
     1141 cgctatgaag tctcaagcga ttgtcacaag tcaagggaga ttgtttcttt ggatatcact
     1201 gtgaactatt gtcatgatat gaagttgttc aaaatgagtc gcagaaatat cggacaagct
     1261 ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc tcaagtacaa
     1321 actccacgta aatccagcaa actcaagccg ctaacagttg aagataaaac ctataaccat
     1381 gcgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa aacgtttaaa
     1441 atgatttcaa caacctatcg aaatcatcgt aaacgcttcg gattacgaat gaatttgatt
     1501 gctggcatta tcaatcatga actaggattc tagttttgca ggaagtctaa taaagaaact
     1561 agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc tctatttgat
     1621 gatgaagtct atgaagatta ttatctagag tttgagaaag aagagacttg ctctgttcca
     1681 cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg gctagaggga
     1741 caaaaagaaa tagatcttag ttatgctctt ttcagcaaag atgcagtgac tttggatgaa
     1801 ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataggggatt gaaagtgcat
     1861 tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc tttcattacc
     1921 tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt agaagataag
     1981 aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga gatagaagtt
     2041 ttataaaaaa gtaaaaaata aataacaaaa acaaacacaa actgttgact tcttcaaaca
     2101 atagtagtat attatgtttg tgaggactaa ttgactgttt tttaaagaca gccaatacac
     2161 tgctggaatc cagcatagaa aaaataaaaa ggagtataca atatgtctat tgttatcggt
     2221 gcagatgctg caggtttgag attgaaagaa gttgtgaaag acttcttaga aaaagaaaac
     2281 ttccaccttg tggatgttac agctgaaggt caagactttg ttgatgtgac tctcgctgtt
     2341 gctgcagaag taaacaaaga agaacaaaac cttggtatcg tgattgatgc ttatggagct
     2401 ggtccattta tggttgcaac taagatcaaa ggaatggttg ctgcagaagt ttctgatgaa
     2461 cgttcagctt atatgactcg tggccacaac aactcacgta tgatcactat gggtgcacaa
     2521 cttgttggtg atgaattggc taaaaacatc gctaaaggat ttgttaatgg taaatacgac
     2581 ggtggtcgtc accaaatccg ggttgacatg ttgaacaaaa tgggctaatt agattatagt
     2641 aagaaaggta agttaaaaat gagaattgca attggatgtg accacatcgt aacagatgaa
     2701 aaaatggcgg tttcagaatt tttgaaatca aaaggatatg aagtcattga ctttggtacc
     2761 tatgaccata cacgtactca ctacccaatc tttggtaaaa aagtagggga agctgtaact
     2821 agcggtcaag ctgatcttgg agtatgtatc tgtggtactg gtgttggtat caacaacgct
     2881 gtaaataaag ttccaggtgt tcgttctgcc ttggttcgtg atatgacaac agccctttat
     2941 gctaaagaac aattgaacgc taacgttatt ggttttggtg gtaaaattac tggtgaattg
     3001 cttatgtgtg atatcatcga agctttcatc catgctgaat acaaaccaac tgaagaaaac
     3061 aaaaaattga ttgcgaaaat tgaacatgtt gaaagtcaca atgctcaaca aacagacgca
     3121 aacttcttta cagaattcct tgagaaatgg gatcgtggag aataccacga ctaagaggtg
     3181 acctatgatt ttaacagtca caatgaaccc atccatcgat atttcctatc ccttggatga
     3241 gttgaagatt gatactgtca atcgtgtggt ggatgtaacc aaaacggctg gtggtaaggg
     3301 actcaatgtt acccgagtac tttcagaatt tggcgattct gttcttgcta ctggtttagt
     3361 gggtggcaaa cttggtgagt ttttggttga acatatcgat aatcaagtaa agaaagattt
     3421 cttctcaatt aagggagaaa ctcgtaactg tatcgctatt ctccacggag acaaccaaac
     3481 agaagttctt gaaaaaggtc ctgaagtatt ggaacaggaa ggtcaagagt ttttggaaca
     3541 tttcaaaaaa ctcttggagt cagttgaagt agtagccatc tcaggtagtc tgccagctgg
     3601 ccttccagtt gattactatg cgagcttggt agaacttgct aatcaagctg gcaagcctgt
     3661 agtcttggac tgctcaggtg cagcacttca ggctgttctt gaatcacccc ataaaccaac
     3721 agtcatcaaa ccaaataatg aagaattgtc tcagcttctt ggaagagaag tttctgagga
     3781 tttggatgaa ttaaaagaag tacttcaaga acctttgttt gcagggattg aatggattat
     3841 cgtttcactt ggtgccaacg gtacttttgc caaacatggt gacactttct acaaggtaga
     3901 tattcctaga attcaggtgg taaatcctgt tggatctgga gactctactg tggcaggaat
     3961 ttcttcagga cttcttcaca aagaatcgga tgcagaatta ctcatcaagg caaatgtcct
     4021 tggtatgctc aatgctcaag aaaaaatgac tggtcatgtc aacatggcca actatcaagc
     4081 tctatatgat caattaatag taaaagaggt ataaaatggc tttaacagaa caaaaacgtg
     4141 tacgcttaga aaaactttct gatgaaaatg gtatcatctc agctcttgca tttgaccaac
     4201 gtggtgcttt gaaacgcctc atggctcaac accaaacaga agaaccaact gtggcccaaa
     4261 tggaagaact taaagtcttg gtagcagatg aattgactaa atatgcttca tctatgcttc
     4321 ttgaccctga gtatggactt ccagcaacta aagctcttga tgaaaaagct ggtcttctcc
     4381 ttgcttatga aaaaacaggt tatgacacaa caagcacaaa acgcttgcca gactgcttgg
     4441 atgtttggtc tgcaaaacgt attaaagaag aaggtgcaga tgcagttaaa ttcttgcttt
     4501 actatgatgt agatagctca gacgaactca atcaagaaaa acaagcctac atcgaacgca
     4561 tcggttctga gtgtgtggct gaagatatcc cattcttcct tgaaatcctt gcttacgatg
     4621 aaaaaattgc ggatgcaggt tctgtagaat acgctaaagt aaaaccacac aaagttatcg
     4681 gcgctatgaa agtcttttca gacccacgct ttaacattga tgttttgaaa gttgaagttc
     4741 ctgttaacat taaatatgtt gaaggcttcg ctgaaggtga agtagtttat acacgtgaag
     4801 aagcagcagc cttcttcaaa gcgcaagatg aagcaacgaa cttgccatac atctacttga
     4861 gtgctggtgt atcagctaaa ctcttccaag atactcttgt atttgctcat gaatcaggtg
     4921 caaactttaa cggagttctt tgtggccgtg ctacatgggc aggatcagtt gaagcttaca
     4981 tcaaagatgg tgaagcagca gctcgcgaat ggcttcgcac aactggattt gaaaacattg
     5041 acgaactcaa taaagttctt caaagaacag caacttcatg gaaagaacgc gtgtaagaaa
     5101 gtcctcctag tttaggaaca tgaatctaaa aaaatttaaa aaaagttgta tgtaaaggtt
     5161 tacaaaacaa ccgacttgtg ctatacttaa atcacaagtt aatacaaggt gagtgttact
     5221 aagtaatatt aggcatgatc acaggtgaat tagaaatcag ctgattttct agttcatttg
     5281 tggtcatttt ttgtactcat atacctttaa gatataaaag gaggttgaca tgtatcgaat
     5341 tctaaatcca atgaatcaca atgtctcgct tgtcagaaat gataagggag aagaggtgat
     5401 tataattggt aaggggattg cattcggaaa aaggaagggg gatttgattg ctgaaaatca
     5461 ggttgagaaa atctttcgga tgaagaccga agagtccaga gaaaacttta tggctcttct
     5521 caaagatgtt ccgcttgatt ttatcacagt gacctatgaa atcattgata agctatcaaa
     5581 gaaatatcat tatccgattc aagagtatct ctatgtaacc ttgacagatc atatttactg
     5641 ttcttatcaa gctctaactc aaggaaggta caaggatagt aatctgccgg atatttccgc
     5701 taagtatcct gtcgcttttc aaatcgcaaa tgaagctttt gaaatttacc gtcagaagct
     5761 agcagatcat tttcctgagg acgagattat tcggattgct tatcatttca tcaatgccga
     5821 aggtgaaaat gaagtggaac ttgtggagtc gattgataag aggaaagaaa ttctcaggaa
     5881 tgttgaagaa gtgctaaagg gttatgcaat tcaacgaacc aaaaaaaata atcatttcta
     5941 tgatcgcttt atgattcatt tgaattattt cttggattat ttagacagat ctagagatga
     6001 taaccaatca cttctggata tggaagatca tattaaacaa tcctatccaa aagccttcga
     6061 gattggttcc aagatctatg atgtgattac gcaacatacg ggtcttgatt tgtataaaag
     6121 tgaacgagtt tatctagttc tacatatcca acgtttattg tcataaaaat ttatttaaaa
     6181 ctatataagg agaattctat catgaataga gaagaagtaa cattgttagg ttttgaaatc
     6241 gtagcctatg ctggcgatgc tcgttcaaaa ctattggaag ccttgaaggc tgctgaagct
     6301 ggtgattttg aaaaagcgga cgctctggta gaggaagctg gtagctgtat tgcagaggct
     6361 caccacgcgc aaacaagtct attgactaag gaagcttcag gtgaggactt agcgtatagt
     6421 gtgaccatga tgcatggcca agaccacttg atgacaacta tcttgttaaa ggatttgatg
     6481 caccatttga tcgaactcta caagagagga gttaaataat gaacaaacta attgcattta
     6541 tcgagaaagg aaagcctttc tttgagaaac tatctcgtaa tatctatctt cgtgctattc
     6601 gtgatggttt cattgcaggt atgcctgtta ttctcttctc aagtatcttt atcttgattg
     6661 cctttgtacc aaactcatgg ggctttaaat ggtctgatga agttgtagcc tttctgatga
     6721 aaccttatag ctattctatg ggtattctgg ctctcttggt agctggtaca acagctaagt
     6781 cattgaccga ctcagtaaac cgtagcatgg agaaaaccaa tcaaatcaac tatatgtcaa
     6841 cgctgttagc agcgattgtt ggtttgttga tgttggcagc tgatcctatc gaaaatggtc
     6901 tagctactgg attcttgggg acaaaaggtt tgctttcagc cttccttgct gcctttgtta
     6961 ctgtagccat ctataaggtt tgtgttaaga acaacgtcac tattcgtatg cctgacgaag
     7021 ttccaccaaa tatctcacaa gtctttaaag atgtgattcc attcactcta tctgttgttt
     7081 ctctttatgc tcttgattta ctagctcgtc attttgttgg ttctagtgtg gcagaatcaa
     7141 tcggtaaatt cttcgcacca ctcttctcag cagcagacgg ataccttggt attaccatta
     7201 tctttggtgc ctttgccttc ttctggtttg ttgggattca tggtccatct atcgttgaac
     7261 cagctatcgc agctattacc tatgccaatg ccgaagtcaa cttgaacctt ctccaacaag
     7321 gtatgcacgc tgacaagatt cttacttctg gtacacaaat gtttatcgtt accatgggtg
     7381 gtactggtgc gactcttgtt gttccattca tgttcatgtg gttgacaaaa tcgaaacgta
     7441 accgtgcaat cggtcgtgct tcagtagttc caaccttctt tggtgtaaat gaaccaatct
     7501 tgtttggtgc accgcttgta ttgaacccga ttttcttcat tccatttatt tttgctccta
     7561 tcgcaaacgt ttggatcttt aaattcttca ttgaaactct tggaatgaat tcatttactg
     7621 ctaatctacc atggacaact ccagctccac taggtctagt tcttggaact aacttccaag
     7681 tgctatcatt cattcttgct gcccttctaa tcgtggttga cgttgtcatt tactatccat
     7741 tccttaaggt ctatgatgaa caaattcttg aagaagaacg ttcaggtaag tctaatgatg
     7801 aattgaaaga aaaagttgct gcaaacttca acactgcaaa agcggatgct attcttgaaa
     7861 aagcaggtgt cgatgcagca caaaatacca tcactgaaga aacaaatgtc ctcgttctct
     7921 gtgctggtgg aggtacaagt ggtctccttg caaatgctct aaataaggca gcagcagaat
     7981 acaatgtccc tgtgaaagca gcagcaggcg gctatggtgc tcaccgtgaa atgttaccag
     8041 agtttgatct tgttatcctt gcccctcaag ttgcttcaaa ctttgaagat atgaaagcag
     8101 aaacagataa gatcggtatt aaactagcga aaacagaagg cgctcaatac atcaaattaa
     8161 ctcgtgatgg aaaaggtgct cttgcattcg tacaagcgca attcgattaa ggctagggac
     8221 tctgaaatag tctcctatcg ttacggaaat cgctatggcg aatttcctaa tcgccttatt
     8281 aattcgtcgg taaaaagata acgtttttac ctcctcatgt cacaattcag tgacttggta
     8341 caagaagtga gatggagaag catggctcac tgactcctct cctctcactt ttactttatt
     8401 taaatcaaga aataggtgaa aaaaatgaca aaaacacttc caaaagactt tattttcggt
     8461 ggcgcaacag ctgcttatca agcagaaggt gctacacata ctgatggaaa aggaccagtt
     8521 gcttgggata aatatcttga ggataactac tggtacactg ccgaaccagc tagtgatttt
     8581 tacaatcgat atccagttga cctcaagcta gcagaagagt atggtgtcaa tggtattcga
     8641 atttctattg cttggtcacg tattttcccg actggttacg gccaagtaaa tgctaaaggt
     8701 gttgagtttt atcataattt atttgcagag tgtcacaaac gtcatgttga gccttttgta
     8761 actcttcatc actttgacac gccagaagct ctccactcaa atggagactt cttaaaccgt
     8821 gaaaatatcg aacattttgt agactacgct gccttctgtt ttgaagaatt tccagaagta
     8881 aactattgga caacctttaa tgaaattgga ccaatcggtg atggtcaata tttggtgggg
     8941 aaattccctc caggtatcca gtacgacctt gccaaagtct tccaatcaca ccacaatatg
     9001 atggtgtctc atgcacgcgc ggtaaaattg tacaaagaga aaggctataa aggggaaatt
     9061 ggtgttgttc acgccctgcc tactaaatat cctctagatc ctgaaaatcc agcagatgtt
     9121 cgtgcagctg agttggaaga tattatccac aataaattca tcttagacgc aacttatcta
     9181 ggtcgctatt cagctgaaac catggaaggt gtcaaccata tcttatcagt caatggtggt
     9241 agtttggatc ttcgtgaaga agattttaca acattagaag ctgcaaaaga cttgaatgat
     9301 ttcctaggaa tcaactacta tatgagtgac tggatggaag cctttgatgg agaaactgaa
     9361 attatccata atggtaaagg tgaaaaagga agctctaagt atcaaatcaa aggtgttggt
     9421 cgtcgtgtag ctcctgacta tgtaccacgc acggattggg attggattat ctaccctcaa
     9481 ggtttgtatg accaaatcat gcgtgtgaag aaagattatc ctaactacaa gaagatttac
     9541 atcactgaaa atggtctcgg ctataaagat gagttcgttg ataacactgt ttacgatgat
     9601 ggtcgtattg attacgtgaa acaacacttg gagatcttgt ctgatgcgat tgcagatgga
     9661 gctaatgtaa aaggttactt catttggtca ttaatggatg tcttctcatg gtcaaacggt
     9721 tatgagaaac gttatggtct cttctacgta gattttgaaa ctcaagaacg ttatcctaag
     9781 aaatcagctc actggtacaa gaaagtagcg gaaactcaga ttatagacta gtagaattag
     9841 tcattagata tagaatttta gtgagtcaaa aagatgttca aagattttat ccaatctatt
     9901 tatgaaaaaa agtttatatt ataaatttcg aaaaatactc tcaaataccg tgtttgacga
     9961 gtgaagaatt gaaaaatctt ggaaaatggt atgtctcgac tggtaaagaa tggatttgtc
    10021 attcagatga tgatctggaa gaatttaaaa atctattttt aaattttatc aatcctgaag
    10081 aatgggatac tatctccttt gattcagatt ttatgccgtt tcaacaatcg taaccaattt
    10141 ctcaaaaaag ttaaatctta tatttagtac tctgtaaaac tcttatctaa tcacgttgct
    10201 tatactcaat gaaaatcaaa gagcaacttt aaactaggaa gcgagtcgca gatttctcaa
    10261 tgtatagctt tgaggaattg ggcaaaaagt ctttgatata gaaaaaagca tagtatcagg
    10321 tgtttcaaca cctgatacta tgctttttat tgtgggaaga tttacttttt ttcttctgaa
    10381 attgagttgt tacccaggct ctttcagttt attaaggctt gatgacttta atgtgtttag
    10441 atagcttaaa aaggattgaa tcacttagtt tagaatctga aacaatagta tcaagatttg
    10501 atatattata aaaagtataa aaatcaaact tattgaactt actatgatct gcgagtaaat
    10561 attttttatt agaattattt aaagcgatgc gttgagcctc tccctcttcc tcgctaaaag
    10621 tagctagagc tccgttttga ataccattac agctaacgaa agctttagaa aattggagat
    10681 tagagagatt ttgtagggtc aatgtgccaa caaaagcacc tgtaatatcg cgataatttc
    10741 cacctatcaa aatcaaatct gttaattttc gttcgcttaa aatcagaaaa acaggtagac
    10801 tgttggttac gacgcggata ttgtcaatag gcaactcacg cgcaaaaaac tctaatgttg
    10861 ttcctggtcc aatgaaaata gtttctcttt cttctactag actgcctgca aaatgggcta
    10921 tttcttgttt ttctgccgtt tggagggctt gtttttcaat atttgatcgc tcattagtca
    10981 aaagggagtt ggttcgaagt ttttcagctc caccatgcac acgaatcagc aaatctttat
    11041 cagctaattc ctgtaaatag cgccttgcag tcatatctga aacggctatt tcgtccataa
    11101 tctgtttaac tgttatggtt cctttactat ttactatctc taaaattttg gttaattttt
    11161 cttgtttgag catattatca cctcgtttcc tactactatc ttaccataaa caaactcatc
    11221 attcaaatac aaaaacaaca aaatgaaaca aaaacaaaaa tatcgaagtt tgttttcaaa
    11281 actttcgata tttttgttgg gttataactt tgatgtttct agtttacttt ttgatgattg
    11341 agagtgatgg agaattagtc taaaccgtag ttatagtcat cgtcttgcat ggcttcaact
    11401 tcgccaagaa ggtaaccatt tccgacttga gagaagaagt catggttgga agttcctgtt
    11461 gaaataccgt tcataacgat tgggttgaca tcttcagctg aatctgggaa aagtggatct
    11521 tgtcccatgt tcatgagagc tttattggca ttgtagcgaa ggaaggtttt aacctcttca
    11581 gtccaaccaa caccgtcata aagactctct gtgtagcctt cttcattttc ataaagagta
    11641 tagagtaggt cgtacatcca ttctttgagt ttttcttgct cttcttcagg caattcattg
    11701 aaaccaagtt ggaatttgta accaatgtag gttccgtgaa cagactcgtc acgaataatc
    11761 aatttaatga tttctgcaac gttggctagt ttgttgttac cgagatagta gaggggagtg
    11821 aagaaaccag agtagaagag gaaggtttcg aggaagacgc tggcaacttt cttttcaagt
    11881 gggctgccgt ttaggtagat ttcgttgaca atctcagcct tcttttgtag gtaaggattg
    11941 gtattggtcc attcgaaaat ttcttcaatc tcagccttag tattcaaggt agaaaagatt
    12001 gatgagtaag atttagcgtg gacagattcc ataaattgga tgttattgaa aacagcttcc
    12061 tcatgtggtg tacggatgtc tgcgcgaagg gcttgaaccc cagtttcaga ttgcatagtg
    12121 tcaagaaggg ttaaaccacc aaaaactttt ccgaccaagt ctttctcttt gttagatagc
    12181 tttctccagt catccaagtc gtttgataag ggaatacgtg tatcgagcca aaattgctcc
    12241 gtcagttttt cccaagttga tttgtcgatg acatcttcga tggcattcca gttaatggct
    12301 ttgtagtaag tttccattta aaatctcttt ctgtgtttag tattgcgaac tcacaattat
    12361 ttctacttta ccataattct ataggagtat cgcacaaaaa gtcggaagcc cgacttttaa
    12421 aatgttacat aaattatgtt atgacatagt agatttgatt ttatcagtgc tgcttaggga
    12481 aaaataatgt ttctatgcta gaaactaaat cacacagctt tcacattggt tggcgccgac
    12541 ttctccaccg tcatctgtaa aggtacggac gtagtagata gacttgattc ccttgttaaa
    12601 ggcatagtta cgaaggatgg acaaatcacg tgtcgtttgt ttattttccc tcttccattc
    12661 gtaaaggcct tttggaatgt cactgcgcat gaagagggtg agtgaaagtc cttgatccac
    12721 gtgttcagtc gcagcagcgt aaacatcgat gactttacgc atatccatat cgtaggcaga
    12781 agtgtagtaa ggaatggttt ctgtagacaa gccagcagca gggtagtaga ttttaccaat
    12841 tttcttctct tggcgttctt cgatacgttg cgtaatcggg tggatagaag cagaaacgtc
    12901 gttgatatag ctgatagaac catttggcgc tacagcaagg cgattttggt ggtaaagacc
    12961 atcttcttga accttgtcgc gaagttcagc ccaatcagca acaccaggga taaaaacatt
    13021 tttgaagagt tctttaacac ggtctgatgt tggaacaaat tcaccagtta catacttgtc
    13081 aaagtaactt ccgttagcat agtctgattt ttcaaagttg tggaaggtaa taccacgttc
    13141 acgcgcaata ttgtttgact ctaccaaggt ccagtagttc ataagcataa agtagatgct
    13201 tgtaaattca acagactcag gtgaaccata ttcaatgagt tgttgggcaa ggtagctgtg
    13261 cagtcccatg gcaccgagac caaaggtgtg ggcttggcta tttccatggt caatcgttgg
    13321 tacagctacg atatgtgaac tatctgtaac gaaagtaagg gcacgaacca tagcacggat
    13381 agaacgacca aaatcaggtg aagtcatcat gttaaccacg ttggttgaac ccagattaca
    13441 tgaaacatct gttcccattt gaaggaattc ttgagcatcg ttgatcaagc ttggttcttg
    13501 aacttgaaga atctcagaac acaagttact catgataatc tttccatcaa caggatttgc
    13561 acggttagcc gtatcgatgt tgactacata aggatagcca gactcttgtt gcaatttaga
    13621 gatttcagtt tccaaatccc gcgccttgat ttttgtcttg cgaatatttg gatttgcgac
    13681 caattcatcg tatttttcag taatgtcgat gtaattgaat ggcacaccgt attctttttc
    13741 tacagagtaa gggctgaaga ggtacatttc ttcattttta cgagccaatt cgtagaattt
    13801 atcaggtact acaacaccaa gtgatagagt cttgacacgt actttttcat cagcgttttc
    13861 tttcttagtt gaaaggaaag cgatgatatc tgggtgaaag acgttgaggt agacaacacc
    13921 agcaccttga cgttgcccca attggttgga gtaagagaag ctgtcttcaa aaagcttcat
    13981 aacaggaacg acacctgaag cagctccttc atagcctttg ataggtgcac cagcttcacg
    14041 aaggttgctg agggtaattc ccacaccacc accaatacgt gaaagttgaa gagctgagtt
    14101 gatagaacgt ccgatagagt tcatatcatc cgtcacttgg attaggaaac aagataccaa
    14161 ctccccacga cgagcacgtc cagcattcaa gaaggaagga gtagcaggtt ggtagcgttg
    14221 gtggatgatt tcattggcaa tatcgattgc aacagcttca ttcccatcag cgaaataaag
    14281 ggcgttgaag aagacacggt cttccatatt ttcaagatag tattcaccgt cattagtctt
    14341 taaggcatat tgattgtaaa atttataagc tgccatgaat gacttgaatt ggaagttttg
    14401 gtctttgata aattgagcca attcttccaa gaactctgga cggtatttct tgataaaggc
    14461 tgtttcgatg tagttgtgtt caatgaggta attgattttg tctttgattg aatcaaaaac
    14521 catagtgttt ggaactacat tttctttaaa gaaagcatcc aaggcttcct tatctttatg
    14581 aagcatgatt tgtccattaa caggacggtt aatttcgtta ttaagacgga agtaagtcac
    14641 gtcttcaaga tgttttaatc ccataaaatt tcccttatct aattacaaaa gaaaggcttc
    14701 taagttagcc ctaaaagcag tttcttctgg atgatgtact aagattatgc taattgtttc
    14761 agttttcctg gttggaaacc tgaaaagact tcagttggtg tttggataac aggagctgcg
    14821 ctaaaaccga gctctttaac ttgatcgacg tactcaggtt gctcatcaag attgatttca
    14881 cgataagaga cattattact gtccaagaaa cgcttggtca ttttacattg gacacaattg
    14941 tttttagaat aaacggttac cattgtgtaa ctcctcttca aaatttaata ctatcttagt
    15001 atatcagaaa ataaaatttt gtcaatgatt tgaactaaat atagtggtgt tcttttgttg
    15061 tttttatata taaacacaat atatagtgtt tgtattgtga aatagcaatg attatcctat
    15121 aaattgtttt taaagaaact atatcctgtg ttttgttatc taggatagaa gattttcagc
    15181 aagttatctg aaaggaaatt gaaggaaaat ccagaaaatg cttgaaaaaa atcctagaag
    15241 atggtataat actaaattgt aagggttatc acatataact caaaaaaaga aagaacaaaa
    15301 ggagagtcaa actatggctt ctaaagattt ccacgtagtg gcagaaacag gtattcacgc
    15361 acgtccagca acattgttgg tacaaactgc tagcaaattt gcttcagata tcactcttga
    15421 gtacaaaggt aaatcagtta accttaaatc aattatgggt gttatgagtc ttggtgttgg
    15481 tcaaggtgct gacgtaacta tctcagctga aggtgcagat gcagatgacg ctatcgctgc
    15541 aatctcagaa acaatggaaa aagaaggatt ggcataaggg aaatgacaga aatgcttaaa
    15601 ggaatcgcag catctgacgg tgttgcagtt gcaaaagcat atctactcgt tcagccggat
    15661 ttgtcatttg agactattac agtcgaagat acaaacgcag aagaagctcg ccttgatgcc
    15721 gctctacagg catcacaaga cgagctttct gttattcgcg agaaagcagt aggtacgctc
    15781 ggtgaagaag cagctcaagt ttttgatgct cacttaatgg ttcttgctga cccagaaatg
    15841 atcagccaaa tcaaggaaac tatccgtgcg aagaaagtga atgcagaagc aggtctgaaa
    15901 gaagttacag atatgtttat cactatcttt gaaggcatgg aagacaaccc atacatgcaa
    15961 gaacgcgcag cggatatccg cgacgtgaca aaacgtgtat tggcaaacct tcttggtaaa
    16021 aaattaccaa acccagcttc tatcaatgaa gaagtgattg tgattgcaca tgacttgaca
    16081 ccatctgata cagctcaatt ggacaaaaac tttgtaaaag cttttgtaac caacatcggt
    16141 ggacgtacaa gccactcagc tatcatggca cgtacacttg aaattgcagc tgtattaggt
    16201 acaaacaaca tcactgaaat cgttaaagac ggtgacatcc ttgctgttaa cgggatcact
    16261 ggtgaggtta ttatcaaccc aactgatgaa caagcggcag aatttaaagc agctggtgaa
    16321 gcttatgcga aacaaaaagc tgaatgggcg ctcttgaaag atgctcaaac agtgactgct
    16381 gacggtaaac acttcgagtt ggctgctaat atcggtactc caaaagacgt tgaaggtgtt
    16441 aacaacaacg gtgcagaagc tgttggactt taccgtacag agttcttgta catggattct
    16501 caagacttcc caactgaaga tgagcagtat gaagcataca aggctgttct tgaaggaatg
    16561 aacggtaaac ctgttgtcgt tcgtacaatg gatatcggtg gagataagga acttccttac
    16621 ttcgatatgc ctcacgaaat gaacccattc cttggattcc gtgctcttcg tatctctatc
    16681 tctgagactg gagacgctat gttccgcaca caaatccgtg ctcttcttcg tgcgtctgtt
    16741 cacggtcaat tgcgtatcat gttcccaatg gttgcgctct tgaaagaatt ccgtgcagcg
    16801 aaagcagtct ttaatgaaga aaaagcaaac cttcttgctg aaggtgttgc agttgcggat
    16861 aacatccaag ttggtatcat gatcgagatt cctgcagcgg ctatgcttgc agaccaattt
    16921 gctaaagaag ttgacttctt ctcaattggt acaaacgact tgatccaata tacaatggca
    16981 gcagaccgta tgaacgaaca agtttcatac ctttaccaac catacaaccc atcaatcctt
    17041 cgcttgatta acaacgttat taaggcagct cactctgaag gtaaatgggc tggtatgtgt
    17101 ggtgagatgg ctggtgacca aacagctgtt ccacttcttg tcggaatggg cttggatgag
    17161 ttctctatgt cagcaacatc tgtccttcgt acacgtagct tgatgaagaa attagatact
    17221 gctaagatgg aagagtacgc taaccgtgcc cttacagagt gttcaacaat ggaagaagtc
    17281 cttgaacttc aaaaagaata cgttaatttt gattaatcga aaagtccctg caactcagtt
    17341 acagggattt ttttgatatt ttaaaaagaa ttttcaagaa aatctttctt atagaaagat
    17401 tttcttgaaa aagtagtagt cagaacaaaa aatacttaaa tggttcataa aattcttgac
    17461 aagttggaga tttaggagta aactattaac cagttaagta atagagagga gtttctgcaa
    17521 tttaaaaatg aattgcaact agaaatatca aatagaaaga gagtttcgat gaaaattaat
    17581 aagaaatacc ttgttggttc tgcggcagct ttgattttaa gtgtttgttc ttacgagttg
    17641 ggactgtatc aagctagaac ggttaaggaa aataatcgtg tttcctatat agatggaaaa
    17701 caagcgacgc aaaaaacgga gaatttgact cctgatgagg ttagcaagcg tgaaggaatc
    17761 aatgctgagc aaatcgtcat caagataaca gaccaaggct atgtcacttc acatggcgac
    17821 cactatcatt attacaatgg taaggttcct tatgatgcga ttttcagtga agaattactc
    17881 atgaaagatc cgaactataa gctaaaagat gaggatattg ttaatgaggt caagggtgga
    17941 tatgttatca aggtagatgg aaaatactat gtttacctta aggatgctgc ccacgcggat
    18001 aacgtccgta caaaagagga aatcaatcga caaaaacaag aacatagtca acatcgtgaa
    18061 ggtgggactc caagaaacga tggtgctgtt gccttggcac gttcgcaagg acgctatact
    18121 acagatgatg gttatatctt taatgcttct gatatcatag aggatactgg tgatgcttat
    18181 atcgttcctc atggagatca ttaccattac attcctaaga atgagttatc agctagcgag
    18241 tt
//
LOCUS       DAVKWS010000037        18007 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKWS010000037 DAVKWS010000000
VERSION     DAVKWS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.037"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            321..1367
                     /gene="pheS"
                     /locus_tag="VW852_001421"
     CDS             321..1367
                     /gene="pheS"
                     /locus_tag="VW852_001421"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV0272556.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVASQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1367..1864
                     /locus_tag="VW852_001422"
     CDS             1367..1864
                     /locus_tag="VW852_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272557.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFAYDNTEEQLQEYFEEAYSLKTLS
                     TELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGFGLGK
                     QLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDTDWLL
                     RKKLR"
     gene            1941..4346
                     /locus_tag="VW852_001423"
     CDS             1941..4346
                     /locus_tag="VW852_001423"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV0272558.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETDQVAADALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4414..5421)
                     /locus_tag="VW852_001424"
     CDS             complement(4414..5421)
                     /locus_tag="VW852_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0272559.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSAPNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPD
                     IIALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRD
                     ALNALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEIT
                     F"
     gene            5577..5980
                     /locus_tag="VW852_001425"
                     /pseudo
     CDS             5577..5980
                     /locus_tag="VW852_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6105..6371
                     /locus_tag="VW852_001426"
     CDS             6105..6371
                     /locus_tag="VW852_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272560.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6632..8881
                     /gene="metE"
                     /locus_tag="VW852_001427"
     CDS             6632..8881
                     /gene="metE"
                     /locus_tag="VW852_001427"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV0272561.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8945..9811
                     /gene="metF"
                     /locus_tag="VW852_001428"
     CDS             8945..9811
                     /gene="metF"
                     /locus_tag="VW852_001428"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV0272562.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10496..12709
                     /gene="pnp"
                     /locus_tag="VW852_001429"
     CDS             10496..12709
                     /gene="pnp"
                     /locus_tag="VW852_001429"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV0272563.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12725..13342
                     /gene="cysE"
                     /locus_tag="VW852_001430"
     CDS             12725..13342
                     /gene="cysE"
                     /locus_tag="VW852_001430"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV0272564.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13354..14238
                     /locus_tag="VW852_001431"
     CDS             13354..14238
                     /locus_tag="VW852_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272565.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14320..15663
                     /locus_tag="VW852_001432"
     CDS             14320..15663
                     /locus_tag="VW852_001432"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV0272566.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIERGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15656..16042
                     /locus_tag="VW852_001433"
     CDS             15656..16042
                     /locus_tag="VW852_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV0272567.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16046..16930
                     /locus_tag="VW852_001434"
     CDS             16046..16930
                     /locus_tag="VW852_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV0272568.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQALQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17067..17666
                     /locus_tag="VW852_001435"
     CDS             17067..17666
                     /locus_tag="VW852_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272569.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEEIGYNNNLSLYATIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNTK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDTIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            17809..17979
                     /locus_tag="VW852_001436"
                     /pseudo
     CDS             17809..17979
                     /locus_tag="VW852_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5416 a         3471 c         4087 g         5033 t
ORIGIN      
        1 tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga taggtcttgc
       61 aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga aagactagta
      121 gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat atgaaagctg
      181 ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga tgaaaacgga
      241 tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct ttttatctac
      301 gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg cttcgcgaag
      361 aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg caagatttgc
      421 gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg atgaaagatg
      481 tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt gatgtcttga
      541 ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg tctcaactgg
      601 ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt caccgtcacg
      661 ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt tatcaagtcg
      721 tggatggttt tgaagtggag caagactact ataactttga acgtatgaac cttccaaaag
      781 accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc ttgctccgta
      841 cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct aaaggtcctt
      901 tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg acccacagtc
      961 accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg gctgatcttc
     1021 aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa attcgtttgc
     1081 gtccatctta ctttccattc acagagccat ctgttgaggt ggatgtttct tgcttcaaat
     1141 gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt atgggggccg
     1201 gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta tactctggct
     1261 ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc aacgatatcc
     1321 gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga ttagaaaagt
     1381 agaaatggca gatgttgagg tgttggctaa aattgccaaa caaacctttg cgtatgataa
     1441 tacggaagag cagttacagg aatactttga agaggcttat agtctgaaaa ctttgtcaac
     1501 tgagttggga aatcctgact ctgaaaccta tttcattatg catgaggagg agatagctgg
     1561 ttttctcaaa gtcaactggg gaagtgctca aactgagaga gaattagagg acgcttttga
     1621 aattcaacgc ctctatgtgc tacaaaaatt ccaaggattt ggactaggta agcaactgtt
     1681 tgaattcgca cttgaacttg ctacaaaaaa tagtttttct tgggcttggt taggtgtttg
     1741 ggagcataat acaaaagctc aagcgtttta taaccgatat ggttttgaaa aatttagcca
     1801 acatcatttt atggttggtc aaaaagtaga tacggattgg ttactgagaa agaaattaag
     1861 gtaagaagag gattcttcta ttgaaatgat aggaaaggaa aagaaccagt gtggcaatgg
     1921 tcattctgga gaactaaatt atgcttgtat cttataaatg gttaaaagaa ttggtggaca
     1981 ttgatgtgcc atcacaagag ttggctgaaa aaatgtcaac tacagggatc gaggtagagg
     2041 gtgtcgaatc accagctgct ggtctctcaa aaattgtcgt cggtgaggtc ttgtcttgcg
     2101 aagatgtgcc agagactcac ctccatgttt gtcagattaa cgttggcgaa gaagaagagc
     2161 gtcagatcgt ttgtggtgcc ccaaatgtgc gtgctgggat caaggtcatg gtggctcttc
     2221 caggagctcg tatcgctgat aactacaaaa tcaaaaaagg aaaaatccgt ggtttggagt
     2281 cacttggaat gatctgttca cttggtgaat tgggaatttc tgactcagtt gtgcctaagg
     2341 aattcgcaga tggcatccaa atcttgcctg aagatgccgt gccaggtgag gaagtctttt
     2401 cttacctaga cttggatgat gaaatcatcg aactgtctat cacaccaaac cgtgcagatg
     2461 ccctttctat gtgtggggtt gcgcacgaag tagcagccat atatgacaag gcagtcaact
     2521 tcaaagaatt tactttaaca gaaacagacc aagttgcagc cgatgccctt tctgtgggca
     2581 ttgagacaga caaggcacct tactatgcgg cccgtatctt ggacaatgtg accatcgcac
     2641 caagtccaca atggttgcaa aaccttctca tgaacgaagg catccgtccc atcaataacg
     2701 ttgtagacgt gacaaactac atcctgctct actttggtca acctatgcat gcttttgact
     2761 tggacacatt tgaagggact gacatccgtg tgcgtgaagc gcgtgctggt gaaaaattgg
     2821 ttaccttgga cggtgaagaa cgtgacttgg acgtgaatga cctagtcatc actgtcgcag
     2881 acaagccagt agcccttgca ggtgtcatgg gtggtcaagc aacagaaatc tctgaaaaat
     2941 ctagtcgtgt tgtccttgaa gctgctgttt tcaatggcaa atctatccgt aagacaagtg
     3001 gtcgcctgaa ccttcgttct gagtcatctt ctcgctttga aaaaggaatt aatgtggcaa
     3061 cagttaatga agcccttgat gcggcagcta gcctgattgc ggaacttgca ggtgcgacgg
     3121 tgcgtaaggg catcgtttca gcgggtgagc ttgatacttc agatgtagaa gtttcttcaa
     3181 cccttgctga tgttaaccgt gtcctcggaa ctgagctgtc ttatgctgat gtagaagacg
     3241 tcttccgtcg tcttggcttt ggtctttctg gaaatgcaga cagctttaca gtcagagtcc
     3301 cacgtcgtcg ttgggatatc acaatcgaag ctgatctctt tgaagaaatc gctcgtatct
     3361 atggatatga ccgcttgcca accagccttc caaaagacga tggtacagct ggtgaattga
     3421 ctgtgacaca aaaactccgc cgtcaagttc gtaccattgc tgaaggagca ggtttgacag
     3481 aaattatcac ctatgctctg acaactcctg aaaaagcagt tgagttcaca gctcaaccaa
     3541 gtaaccttac tgaactcatg tggccaatga ctgtggatcg ttcagtcctc cgtcaaaata
     3601 tgatttcagg gatccttgat accgttgcct acaacgtggc tcgtaagaat aaaaacttgg
     3661 ccctttatga gattggaaaa gtctttgaac aaacaggtaa tccaaaagaa gaacttccaa
     3721 atgagatcaa cagctttgcc tttgccttga caggcttggt tgctgaaaaa gatttccaaa
     3781 cagcagcagt tccagttgat ttcttctatg ctaagggaat ccttgaagcc ctatttactc
     3841 gtttgggact ccaagtaacc tatacagcaa catctgaaat cgctagcctt catccaggtc
     3901 gtacagccgt gatttcactc ggtgaccaag ttcttggttt ccttggccaa gtgcatccag
     3961 tcactgccaa ggcttacgat attccagaaa cgtatgtagc tgagcttaac ctttcagcta
     4021 tcgaagctgc gcttcagcca gcgactccat ttgtagaaat caccaaattc ccagcagtca
     4081 gccgtgacgt tgcccttctc ctcaaggcag aagtgactca tcaagaagtt gtagatgcta
     4141 tccaagctgc cggcgtgaaa cgtttgacag atatcaaact ctttgacgtc ttctcaggtg
     4201 agaaattggg acttggtatg aagtccatgg cttatagctt gaccttccaa aatccagaag
     4261 atagcttaac ggacgaagaa gtcgcacgct atatggaaaa aatccaagca tcgctcgaag
     4321 aaaaagtgaa tgcagaagtg cgttaaacga tagtaggata caaattcccc ctctagttta
     4381 tgaacactag aggggtgatt ttatataaaa taattaaaat gtgatttctg taaaaataca
     4441 tctatgatca gagttttgaa aacttacaat gtctaagtct ctaacatagt agtggttttt
     4501 aggcaataag atatgatcta tcgttgcatt aaaaagtttt ggactttggc tattccaagt
     4561 tcctctttca aaaggtggaa gtgcattcaa tgcgtcccta tgagagctta tttttgcaag
     4621 tgctccatga cgcatagttg cattaaaatc acctgcaata atagcctttg gatattttga
     4681 agccaattga ttatgaatga tgtttaagtc ttgcttccag atttccatta aacctggcag
     4741 aggaggagca gtatgcaagg caatgatatc tggtatattt tgttttctcg aatgtaatac
     4801 aattgtcccg aaccgtgttg tatgaaaagt tttagcttct gtatagaaac cataactttt
     4861 cttgacaatc acaatcacag tcacaggagc tattccacta ttggtaggtg gagaagtgaa
     4921 aatatcatag ttggccatag aaagtccaac ttgatgaaac aatagtttga ttctctggtt
     4981 ttcttgctca cctctgatat tggtagctag ttcagggaat atagccatat cggcatcaaa
     5041 atggctaaaa attcgctcaa tatgttgtgc ttctatttga ttagcgacgt tccaagttac
     5101 taattttaag gttttagttt tatttggtgc agaagaaagg gttttatctg ttccaaataa
     5161 tagtaatatg agcgatagta agagcaaaat gccagagtaa aaccgtattt gtttattttt
     5221 cttgtaaaaa gatagggaaa aaaatacgaa acttaaaata gaagccatag ttgcagtagc
     5281 taaggggaag gcataaatct gtgccaagat aggtctggta gatagccaaa tattaagatt
     5341 agaagatgga aaaaaagaat aagctatgat agggataaca aagagaatga ccgaaagggc
     5401 agagagaaat gttttaatca ttgtgtgagt acctcaattt gcaatggttg ctcggagcta
     5461 atgtgacttt gtgactttac ttgattgata ttctttttct tgtcttcctt atagattaag
     5521 tacaacccaa gtaaaaaaag tccaatttaa gattgaaatg attaaaaggc acagaaatgc
     5581 aagacaatta tactacaaaa ggcaaacatt tgacaatcga tagccgtcgc ttaatcgaac
     5641 gatggaaaaa agaaggaaaa tcaaatagag aaattgcctc tctacttgga aaagctcctc
     5701 aaactatcca cactgaaatc aagcgtggga cagtccgaca atgtcttgga aaagggcgct
     5761 tcaaagagat ttattctgcc gactacgctc aacagtctta agaaactaaa ggaaaagatt
     5821 ctccactata cataaccaaa aattttcgcc tgaaatgatg gtgatggcta aaggggttaa
     5881 cgtgggaatt tcaaccattt actattggat tcatcgtgga aaattggggt taagcaagca
     5941 ggatctgctt tatcctagaa aaggaaaatc gcttaagaaa taggctagca ccaactttaa
     6001 acccactggt caatctatcg aatcataatt cgctcatttt tttaaaaata catagacgta
     6061 aaaaaagagg gttaagtgat gaagaatagt gctattggga gtaattggaa ggatgtccat
     6121 ctgagctctt tagcaaggag gaaatccttg aaagtgatat gcgagtggct atcatgagcg
     6181 agttgattga ggctaggtat gaacaaggaa tcagtcagaa aaagctggaa gaagtcagtg
     6241 gaataagcca gcctgtcata gctaggatgg agacaggaaa gactagtcct cagttggata
     6301 cagtcttaaa agtcctagcc agtttaggaa agacactagc aatcgtccca cttgaacagg
     6361 gaaaaagttg actagaatca gttatagtct tttctaataa caggtcatag tcacttataa
     6421 gaattactaa taacatgttt tagtattcta attggaatat agctgtaaag taagaattac
     6481 aaaggatttg agacgtttgc ttcaagtctt tttcttttgt ccgaaacaga tatatagttt
     6541 caaactatat caaaccctaa ttttttacaa ttatacagac gctttctttt ctgttaagat
     6601 agtttcaaca acaaattttg gaggacacat catgtcaact acaatcatcg gtttccctcg
     6661 tttgggcgaa ttccgtgaat taaaatttac aactgaaaaa tactttagaa aagaaatctc
     6721 agaagaagaa cttttagcag cagcaaaaga cttgcgtgct aaacactgga acattgtcaa
     6781 agaaaaaggc atcactgaaa ttccatcaaa tgacttttct cactatgata acttcctaga
     6841 tgcggctttc cttttcaacg tggtgcctgc atccgttcaa aacttggact tgtctgacct
     6901 tgagcgctac ttcgctttgg gacgtggtta ccagggagaa aaaggggacg ttcgcgccct
     6961 tccaatgaag aaatggttca acactaacta ccactacatc gttcctaaat ttgaaaaaga
     7021 cactcaagta aaattggctg gtcacaagat tttcgatgag ttccaagaag ccaaagaact
     7081 tggattgaac actcgtcctg ttcttgtagg tcccttcact ttccttcaat tgtcagactt
     7141 tgaagaaggc gtgaaagcag acgacttcgt agatagctta gtggctgctt accaagaagt
     7201 ttttgctaaa ttggctgaac ttggtgcgac tcgcatccaa ttggatgaag cggctcttgt
     7261 aaaagacttg acagctgaag aaaaagctct cttcttgaac ctttacaaca aactcttggc
     7321 tgacaaaaaa ggtcttgaag tcttgcttca aacttatttc ggtgatgttc gtgacgtata
     7381 cgctgacctt gtaaacttac cagtagatgc tatcggtctt gactttgttg aaggtaagaa
     7441 aactcttgaa ctagttaaag gtggcttccc agctgacaag actctctatg taggtattgt
     7501 caatggtaaa aacatatggc gcaacaacta cgaaaagagc ttggctgttc ttgagcaaat
     7561 cccagctgaa aacatcgttt tgacaagctc ttgctcactt cttcatgtgc catttacaac
     7621 tgctaatgaa gaatttgaac cagcactctt gaaccacttt gcctttgcag ttgaaaaatt
     7681 ggatgaaatc cgtgacttgg atgctatccg caatggtcaa ggttcagaag ctcttgcagc
     7741 caacaaagaa ctctttgcga ctgagcgtgt tggtgaaaat gctgaacttc gtgcgcgtat
     7801 cgctggcttg acagacgcag actacactcg tttgccagcc tttgcagaac gtgaagctat
     7861 ccaagaagaa gctttcaaac ttccagctct tccaacaaca acgattggtt cattccctca
     7921 aacaaaagaa gttcgtgcca aacgtttggc ttaccgtaaa ggtgaattgt ctcaaaaaga
     7981 gtacgatgct ttccttgctg aaacgattga cgaatggatc aagtggcaag aagatattga
     8041 ctttgatgtc cttgttcacg gtgaatttga gcgtaatgac atggttgagt acttcggtca
     8101 aaacttgtca ggatacctct tctctaaaaa tggatgggta caatcatacg gtatgcgtgg
     8161 ggtaaaacca ccaatcatct ggggtgatgt cactcgtctt aaccctatca ctgttaaatg
     8221 gtctagctat gcacaaagtc gtacaaacaa acctgttaaa ggtatgttga ctggacctgt
     8281 taccatcctt aactggtcat tcccacgtga agacatctct atcaaggatt ctactcttca
     8341 aatcgccctt gctatcaagg atgaagtgct tgaccttgaa gctgctggtg tgaaaatcat
     8401 ccaaattgat gaggctgctc ttcgtgaaaa attgccactc cgtcgtagtg actggtacga
     8461 agattacctt gactgggcaa ttcctgcctt ccgcttggta cactcaacag tagcgtcaga
     8521 cacacaaatc cacactcaca tgtgttactc agaatttaca gatatcatcc cagctatcga
     8581 taacatggat gcagacgtta tttcctttga agccagccgt tcaaaccttg aaatcttgga
     8641 cgaactcaaa gcgaaaaact tccaaacaga agtgggacct ggggtttacg atatccactc
     8701 acctcgtgta ccaaatgaag gcgaaatcga caacacaatc gaagccatcc ttgctaaagt
     8761 gccaagcaag aaagtttgga tcaaccctga ctgtggtttg aaaacacgtg gtatcccaga
     8821 aacaaaagaa agcttgatcc gccttgttga agcagctaaa gctgcgcgtg agaaattgta
     8881 agattggatt tctctccata cagtggttgt tcaaaaagaa atcaactaga aaaggttatt
     8941 acatatgtca cgccaaacac cgtcactctc atttgaagtg ttccctccaa acccagccgt
     9001 gggtaatggt aacattattt cagccttgca ggatatgcag gagttggctc cccattttat
     9061 cagtgtaact gccagcaata ataaatttaa tatcaaggaa acgacggttc gtttggctga
     9121 ctttatccaa aatgatttgg cgattccgac cattgcccac ttgccagcta tttatctaac
     9181 taaggacaag gttgctgaaa ctattgctga cttggacaaa gttggggtgc agaaaatctt
     9241 ggctcttcgt ggggatatta ttccagacgt ggaaccacaa aaggatttcc gctatgcaac
     9301 cgacttgatt gagtttatca aggaacaaac ccctcacttt gatattattg gagcttgcta
     9361 tccagaaggg catccagatt cgccaaatca gatttcagat attcaaaatc ttaagaagaa
     9421 agtggatgca ggctgttcga gcctcgtaac tcagctcttc tttgacaatg agcgcttcta
     9481 tgattttcag gacaagtgca tcttggctgg gattgatgtt cccattcatg caggaattat
     9541 gccaattctg aatcgaaatc aggctctccg actcttgaag acttgtgaga atatccatct
     9601 tccacgcaaa tttaaagcca tcttagacaa gtatgagcat gaccctgagt cgctcagagc
     9661 agcaggactt gcctatgcag tggaccaaat cgtggacttg gtaactcagg atgttgccgg
     9721 tgtgcatctc tatacgatga ataatgctga tacagcaaaa tacatccatc aagcaaccca
     9781 tgccttgttt aatcaccagt ctctaggata ataaaagcaa accattcttc tcaggtgagg
     9841 ggaatggttc ctttttaatg gtaaagacct cactttttaa gaaaaatatg ataaaataaa
     9901 ttctgtacgt acttgataca aagatgaagg tattaaataa tacaatgcat ttaaactgta
     9961 agatttaacg acacgagtga gtcaaatcga tgttatttga cgaaagagtt agtaaagcat
    10021 ttagctaatc gcccgatagc gattggcgtg aaaggtttag atactttaat attaaatctt
    10081 tctaatgatg tatcttgaaa actgaacacg cctacaactc tgtggaaaaa gataaatctg
    10141 cctaggagca gtcgctccgt cgttcgattt cctattttcc tttgagttgc ttgacggctt
    10201 agcatcttga gtaattgaat tttggtctca tttcttagga aaaaagataa gcttcctagc
    10261 actcgtcgag tgcgacgtca ccttcctatt tttctacaga aatgttcggc aagccgaacc
    10321 gtccaaaata tcttgtgtaa ttgaacacgg ccgaaaagct gtgtaaaaaa gataaactgt
    10381 tttgtcttca tcgaagactt cgtcagtttc ctatttttac tttgcttttg acgtccttgg
    10441 tatcttgatc tttgtaggca aggcgtataa tttcatcaat ccaaagggga ttaaaatggc
    10501 aaaacaagtg tttcaaacga cttttgcggg tcgtgagtta attgtagaga ctggtcaggt
    10561 tgctaagcaa gcaaatggct ctgttgtcgt acgttacggt gagtcaactg tcttgactgc
    10621 tgccgttatg tctaagaaaa tggcaactgg ggatttcttc cccctccaag tcaactacga
    10681 agaaaaaatg tatgcggctg ggaagtttcc tggtggcttt atgaaacgtg aaggacgtcc
    10741 ttcaacagat gcgaccttga cagcgcgttt gattgaccgt ccgattcgtc ctatgtttgc
    10801 ggaaggtttc cgtaatgaag tccaagtcat caatacagtg ctttcttatg atgaaaatgc
    10861 atctgcacca atggctgcta tgtttggttc atctttggca ctgtctattt cagatattcc
    10921 atttgacgga ccaattgctg gggtacaagt gggatatgta gatggccaaa tcatcatcaa
    10981 cccaagtcaa gaacaagcag agcaatctct tcttgaattg acagtagctg gaaccaagca
    11041 cgctatcaac atggtagagt ctggtgccaa agaattgtca gaagaaatca tgttggaagc
    11101 gctccttaaa gggcacgaag ctgtcaaaga attgattgcc ttccaagaag aaatcgttgc
    11161 tgctgtcggt aaagaaaaag cagaagtgga attgcttcac gtggatgctg aattgcaagc
    11221 tgaaatcatt gcagcctaca acagtgacct ccaaaaggca gttcaagtag aagagaaatt
    11281 ggcccgtgaa gctgcgactc aagtagtgaa agaccaagtg actgccgttt acgaagaaaa
    11341 atatgcggac cacgaagaat ttgaccgtat tatgcgtgat gtggctgaaa tcttggaaca
    11401 aatggaacac gcagaagtgc gacgtttaat tacagaagac aaggtgcgtc ctgatggtcg
    11461 taaggtcgat gaaatccgtc ctttggatgc ggttgtcgac ttccttcctc gtgtacatgg
    11521 ttcaggtctc tttactcgtg ggcaaactca agctctttca gtcttgacct tggctccgat
    11581 gggagaaact caaatcattg atggtttgga tccagagtac aagaaacgct ttatgcacca
    11641 ctataacttc cctcaatatt ctgtagggga aacaggtcgt tacggtgcgc caggtcgtcg
    11701 tgaaatcggt cacggtgccc ttggtgagcg tgctcttgct caagtcttgc caagcttgga
    11761 agaattccca tacgctatcc gtctagtagc agaagttttg gaatcaaacg gttcttcatc
    11821 tcaagcttct atctgtgcgg gaactcttgc ccttatggct ggtggtgtgc caatcaaggc
    11881 gccagtagct ggtattgcta tgggacttat ctcagatgga aataactaca cagtattgac
    11941 agatatccaa ggtttggaag atcactttgg agatatggac ttcaaggttg caggtactcg
    12001 tgatgggatt acagcccttc aaatggatat caagattcaa gggattactg cagaaatctt
    12061 gacggaggct cttgctcaag ccaagaaagc gcgttttgaa atccttgatg tcattgaagc
    12121 aaccattcca gaagttcgtc tagaattggc tccaactgct ccgaaaattg atacgatcaa
    12181 gattgatgta gacaagatta agattgtcat cggtaagggt ggagaaacca tcgacaagat
    12241 tatcgctgaa acaggtgtta agattgatat agacgaagaa ggaaatgtgt ctatctactc
    12301 tagtgaccaa gatgctatta accgtgccaa agaaattatt gctggtttgg ttcgtgaagc
    12361 caaagtggat gaagtttacc gtgctaaagt cgttcgtatc gagaaatttg gtgcctttgt
    12421 taacctcttt gataagacag atgcccttgt tcatatctct gagatggctt ggactcgtac
    12481 caatcgtgta gaggatttgg tagaaatcgg ggatgaagtt gatgttaagg ttatcaagat
    12541 tgatgaaaaa ggccgtatcg atgcctctat gaaggctctt ctacctcgtc cgccaaaacc
    12601 tgagcatgat gaaaaaggtg aaaagtctga gcgccctcac cgcccacgtc atcacaagga
    12661 tcacaaacct aagaaagaat ttacagaaac accaaaagat tcagagtaag aaaaggagaa
    12721 atgtatgggg tggtggcgcg aaaccattga tatcgtaaaa gaaaatgatc cagcggcccg
    12781 caccactttg gaggttttgc tgacttatcc aggtgtcaag gccttggcgg cccaccgtct
    12841 ctcgcatttt ctctggaagt acgacttcaa actcttagct cgtatgcaca gtcagttttg
    12901 gcgcttttgg actcagattg agattcatcc aggagcccag attgattcag gtgtctttat
    12961 tgaccatggt tctggtctgg tgattggaga gacagcgatt gttgaaaaag gcgttcttct
    13021 ctatcacgga gtgactctcg ggggaacagg gaaagactgt ggcaaacgcc atccgactgt
    13081 acgaaaggga gccctcatat cagcccatgc ccaagttatc gggcctgtgg aaattggtga
    13141 aaatgccaag gtcggtgctg cagcagttgt cgtagcagat gtacctagtg atgtgacggt
    13201 tgtcggtatt ccggccaaga ttgtccgtct tcatggtaag aaagatgagc ctgttattca
    13261 cgaagtcgaa gaaaaacggg agtattatgt caataaactc gagcaggcta aagatgccag
    13321 tcacagatcg tctggtttgt agaggatacc tatatgattc aagcaagaaa caagttaagc
    13381 caagaggagc tatctgaggc gaaaaaagta attaactgtt gccaaaacta tgacggtacc
    13441 tatcgcgatc cctatctctc taacatgctt aattttgacc caaacatgcc cgcctttttc
    13501 ctttattatg aaaaaggcga acttgttggt ttattaactg tctatgcaga tgaccaagat
    13561 gtagaagtga cgatactggt tcatccaggt catcgccgtc aggggattgc gcgtgcattg
    13621 tttactagtt ttgagagaga aacagcttct ttccccattc gatcagtcac ttttcagaca
    13681 gaacgtattt ttttagagaa tcatcctgac tttgccagta actggggact aatcgaggat
    13741 gaagagacag aaacttggtt aggtaaggat agaagacctt atccgttagc aaatgtttcc
    13801 catcttgaaa ttttgttagc agatagttcg tatcaggatc aaattagtca attaaaattt
    13861 caggcatttt cagaggaaca tgaatcgaga gaagttgtgg atagatatgt cgctgaagct
    13921 ctgaaagatc cagaaagccg actatatatt ttattaaaag ctggtcaggt tattggaact
    13981 tgtacggttg atttatcgac taatacgaat tacctctatg gtttagcaat attggaacct
    14041 gaacgtggaa aaggctatgg aagctactta gcaaaatccc tcgtcaacca actaattgag
    14101 caaaatgaca aggaatttca gattgcagtg gaagatagca atgtaggtgc caaacgtttg
    14161 tatgaaaaaa ttggctttgt caaacagact caggtggttt atctgaatga gaaaggagca
    14221 agggattccg aagtgtagag atattcggac tgaaatttac ttgaactttt agtgatgaaa
    14281 ctaattgttc ttggatttca gctttcctga ttatgattta tgattaaaat ctatgacacc
    14341 atgtctcgtg atttgcgaga atttgtcccg attgaggacg gcaagatcaa gatgtatgtt
    14401 tgtgggccaa cggtgtacaa ctatatccac gtgggaaacg cccgttcgac ggtagctttt
    14461 gatacgattc gtcgctattt tgagtaccgt gggtataagg ttgcctatat ttccaatttt
    14521 acagatgtgg atgataagat tatcaaccgt gccagggaag aaggtatcac gcctcaggag
    14581 gttgcggata agtacatcgc tgcctttcgt gaggatgtga cggccttagg cgtgaaacct
    14641 gcgactcgcc atccgcgtgt agtggagttt atggcagaca tcatccgttt tgtggaagac
    14701 ttgatcgaga gaggctttgc ctatgagagt caaggggatg tctatttccg tgtagaaaaa
    14761 tcccacaact atgctaaatt ggctaataaa accttggaag atttggatct aggtgcttca
    14821 ggtcgtaccg atgaagaaac ggctcgtaag gaaaatcctg tagattttgc tttatggaaa
    14881 tcttccaaac caggtgagat ttcttgggat agtccttggg gacctggacg tccgggttgg
    14941 catattgagt gttcagtcat gtcgacagag attttaggtg ataccattga tatccacggt
    15001 ggtggagctg acctagagtt tccacaccac accaatgaaa ttgcccaatc tgaagctaaa
    15061 acaggcaagg cttttgctaa ctactggatg cacaatggct ttgtcaatat cgacaatgtc
    15121 aaaatgtcta agtccttggg taacttcatt acggtacacg atgcccttaa aactcttgat
    15181 gggcaagtac ttcgtttctt ctttgcgacc cagcactacc gtaagcctat caacttcacg
    15241 gaaaaggcag ttcgcgatgc cgagaccaat ctcaagtatc tgaagaatac ttacgaacaa
    15301 ccatttacag gaaacgtgga tgcccaagag ttacaaaact ttaaagataa gtttgtagca
    15361 gctatggatg aagattttaa tgctgccaac ggtatcacag ttgtctttga aatggccaaa
    15421 tggatcaact cagggaacta tgatgcaagt gtcaagcaag ctcttgcaga tatgttagaa
    15481 atttttggaa ttgtctttgt tgaggaagtt ttggatgcag agattgaaga cttgattcaa
    15541 aaacgccaag aggcgcgtgc caatcgagac tttgagacag cagaccaaat ccgtgaccaa
    15601 ttggttactc aaggaattaa gctccttgat accaaggatg gagtgaggtg gacacgtgat
    15661 tgatgtcaat ctcattaacg ggattgcgct agcctttgag ggggatgcgg tgtattctat
    15721 gtatattcgc cgtcacctca tcctcaaagg tatgaccaaa cccaataaac tccatcaaga
    15781 agcaactaag tacgtgtcag ccaaggctca ggctcgcctg attgctctca tgttggagga
    15841 gcaggtccta acggaaaaag aagaagaaat ctacaaacgt ggccgcaata ccaatagcca
    15901 cacaaaggct aaaaatgcag atgtcgtgac ttatcgtatg tccacgggat ttgaagcggt
    15961 tatgggctat ctccatatga ctgagaatct ggaacgtctt gagagtttgg tttcatggtg
    16021 catccaaaaa gtggagggct agaacatgag ggcaaaagaa ctacaagact ggtttcctga
    16081 ggctcggatt tcagaccaac cagtagagaa agagggctat ctcacgctcc ctttagcttc
    16141 tcagcagtgg attttgctgg aggaagctgg gctcagcgag cgtgaaaagc agttggttgc
    16201 ccttttgacc cagcaggagc aggctcgttc gctaaaccct tggtattcct atctggttga
    16261 gggcaaggga caggcactgc aagtttttaa aaagattcag ttggtttatt gccatctttc
    16321 ttattttcag caggaaaatc tggcttcttg gctagatatg atgcggactc tttttccgaa
    16381 ttgtcagaca gtgctacagg tcggagctca ggattatgtt ttcgtgcttc aacaagacaa
    16441 atacacttct gtaagagata ttttaagtga tacgattgaa gcggttgagt atgactttgg
    16501 acttcgtctt tctatcatgt tgggtcaggt ttggtctcag acgggacatc aagccctatc
    16561 agacttaatc aaagctgagc gggatttgtt caagacatgg tggcgtcagg gtcaccaagg
    16621 tgttcatact ttttctcaac tctatctttg gagtatggga gaaagactcg tggacttgaa
    16681 gccaatcaag gaatgtctac accagatgat tttggatcaa gatcagattc aggaaatcat
    16741 tctctctctt tgggaaaata gtgctgttct cactaaaaca gcccagcaac tctatctgca
    16801 ccgcaattct ctccaataca agattgataa atgggaagag ttgacagggc ttcagttgaa
    16861 agagttgacc gacctgacct tgtgttatca attgatttta ggttctttgt caactatagt
    16921 tggtttgtaa agaagttaat atttggagaa gaggatggcc atcttctcca tttttatgtg
    16981 cagagttata gtggcttgat gctgggaaag tacactgtga ctgctaaaac attcctagaa
    17041 gttgctttga tttccctaat ctatttatgc aaattttatg ttattttact ataacagttg
    17101 ttgctaagcc aaataatagt ggggaagttc atttagacgt aagcattgaa gataatcagg
    17161 gaggtagtgg gcataatttc agttctgttt caagtagctc acaaacagct aaatatgaag
    17221 aaattggtta taataacaat ttatcattat atgcaacgat tgataaaacg tctgatgcaa
    17281 cagctctttt gaaattaaag ttgaataatg ttgataatca acctgctact gaagttccta
    17341 gttcaggaat tactgtaaaa ttaaatacta aagataatgc tggaaactgg acaagtgctt
    17401 cgaataaaaa agaaataaca gtaaaaattg tttctgctaa accgacatat ccagacaaaa
    17461 tcttagtgaa aaatcctgat actataaaag atacagaaaa aaatgccatt attgaaaaat
    17521 tgaaagaggc aaataaaaat catccagcag gagctccaac ctttgctaaa ggtgaaggag
    17581 agcatgcaaa tgatattgta gcaacttatt cagatggtac aacttattat gtaccgttaa
    17641 atgatgtgac aaaatatgcg aggtagtggc tgtaccactc acttattcac ctccccgtga
    17701 tttgtagtag tgataggctt tctcactatt attataaaac aaaataaaga tcacaacact
    17761 ttttcattct gtgttgtgcc ttgagtgaaa cgaaaggaat gaattataaa tatgaaaagt
    17821 atagtcacta gcatagatga gcgcttgcgt actcgcctac gagtgattat ctggaagcaa
    17881 tggaagaaga aatcgagacg attatgggga ttgcttaagt taggggttcc taaatggata
    17941 gcagataagg tatctggctg gggcgaccac tatcaattat agtaaaatga aataagaata
    18001 ggacgaa
//
LOCUS       DAVKWS010000038        17387 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKWS010000038 DAVKWS010000000
VERSION     DAVKWS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.038"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(506..1642)
                     /locus_tag="VW852_001437"
     CDS             complement(506..1642)
                     /locus_tag="VW852_001437"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV0272570.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(1704..2141)
                     /locus_tag="VW852_001438"
     CDS             complement(1704..2141)
                     /locus_tag="VW852_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0272571.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(2143..4098)
                     /locus_tag="VW852_001439"
     CDS             complement(2143..4098)
                     /locus_tag="VW852_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0272572.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIGVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(4122..5879)
                     /locus_tag="VW852_001440"
     CDS             complement(4122..5879)
                     /locus_tag="VW852_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV0272573.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKGQLVSTSVDAVVSTDSVEKIIFACDAGMGSSAM
                     GASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHVSVDN
                     FLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQGTAT
                     MGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNSQFLI
                     VDSLVTTPEYDKMAARMYK"
     gene            6088..7300
                     /locus_tag="VW852_001441"
                     /pseudo
     CDS             6088..7300
                     /locus_tag="VW852_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7428..8450)
                     /gene="cbpF"
                     /locus_tag="VW852_001442"
                     /pseudo
     CDS             complement(7428..8450)
                     /gene="cbpF"
                     /locus_tag="VW852_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(8469..9290)
                     /gene="cbpG"
                     /locus_tag="VW852_001443"
     CDS             complement(8469..9290)
                     /gene="cbpG"
                     /locus_tag="VW852_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV0272574.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(9371..9610)
                     /locus_tag="VW852_001444"
     CDS             complement(9371..9610)
                     /locus_tag="VW852_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272575.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(9760..10026)
                     /locus_tag="VW852_001445"
                     /pseudo
     CDS             complement(9760..10026)
                     /locus_tag="VW852_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10117..10362)
                     /locus_tag="VW852_001446"
                     /pseudo
     CDS             complement(10117..10362)
                     /locus_tag="VW852_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10287..10518)
                     /locus_tag="VW852_001447"
                     /pseudo
     CDS             complement(10287..10518)
                     /locus_tag="VW852_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10519..11151)
                     /locus_tag="VW852_001448"
     CDS             complement(10519..11151)
                     /locus_tag="VW852_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272576.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(11165..12160)
                     /locus_tag="VW852_001449"
     CDS             complement(11165..12160)
                     /locus_tag="VW852_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0272577.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKFPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERIEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(12157..12855)
                     /locus_tag="VW852_001450"
     CDS             complement(12157..12855)
                     /locus_tag="VW852_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV0272578.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(12932..13942)
                     /locus_tag="VW852_001451"
     CDS             complement(12932..13942)
                     /locus_tag="VW852_001451"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV0272579.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(13926..14933)
                     /locus_tag="VW852_001452"
     CDS             complement(13926..14933)
                     /locus_tag="VW852_001452"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV0272580.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(14920..15873)
                     /gene="mvaD"
                     /locus_tag="VW852_001453"
     CDS             complement(14920..15873)
                     /gene="mvaD"
                     /locus_tag="VW852_001453"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV0272581.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(15855..16733)
                     /gene="mvk"
                     /locus_tag="VW852_001454"
     CDS             complement(15855..16733)
                     /gene="mvk"
                     /locus_tag="VW852_001454"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV0272582.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(16855..17184)
                     /locus_tag="VW852_001455"
     CDS             complement(16855..17184)
                     /locus_tag="VW852_001455"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0272583.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFCKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFHRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVR"
BASE COUNT         5365 a         3800 c         3034 g         5188 t
ORIGIN      
        1 tgtcctgttc ttgtttcatt ttactatata gatcgcattc gaaagaattc gttccaaaat
       61 tgcttggttc gttcttctct tggacgacta aagaattgat gggcattatt tttttcaaca
      121 attttcccac agtccataaa aataacatga tgggctacat ctctagcaaa acctatctca
      181 taaagctgat caaaaactca atctgaggag aaaatccaat aaggctttcc ataataaagg
      241 acatcctatc aagatttttg aacacccgat aggctgcctt ttcccgattt tgaagacttt
      301 ttgtccagct tcttttacct tataaataac aacaaaaatc aaagacaagc tagaaagatt
      361 accgcagata actcaaacag tgttttgagg ttgcagataa gactgacgga gtcagctcaa
      421 agcactgctt tgaggttgta gatagaacaa catatgccta agacaaagcg atgctaacct
      481 gatttgaaga gattttcgaa aaagattaaa tatactctac aatttgctca atcaattctt
      541 ggtcgtctaa acctgtaact tgtataacaa catctttgac tgattgttta gccaacaatt
      601 cacctaatcg aatactttct tcatcattta catcgcgata gtcaaaggca tagccaactg
      661 ttttaagtag gtttttatat gacaaactga gttctttcaa ttcacgtatc ggccggatga
      721 atcgttcatt atagcctaat tttcggattg gagtacgagc tacgcgacta acctcgtcca
      781 ctatgaaagg attttcaaac cgttctataa tgactttgtg ataattctcc aattcttttt
      841 tatcaaagtt ccatttggca atcaagagac tccgaatttc agctaataca gattcaatcc
      901 gagatttgat attaggattt tgaagagctt ccaaaattgt cttggcacca taatgcgcac
      961 caatgtaagc tgaagttgca tgtccagaat tgactgaaaa aagttttcgc tcaataaagg
     1021 gttctaaatc ttcttcataa tgcacatctt ttagacgtaa atctggattt ttaagacgct
     1081 tggtttccac gacccattca ttaaagggct cgaccacaac aaaaagggaa tcttcgtgac
     1141 tttgtgctgg aacaatcctg tctactgcag catttggaaa acctatgtag ttatcagcaa
     1201 atgtcaaacc ttccggactt aaatatttct tgacttcttg ataaagaaat tgagacccgc
     1261 caatcatatt ttcacaggcc ataacatcca atacctgtgt attccctgca actcggcgag
     1321 cttcgattcc tttggctaga agttcagcga taaaagggag tatattaggt ccgattgcag
     1381 tagtaataat atccgtcttt tgaatcgctt caatgacttg ctcaggatgt tctttgctat
     1441 taatgccagc cacattagtt acttctatac gagactgtcc tttctgtgca atttcaattt
     1501 catacttgcc cttttcattt agagcatgaa ttatctgatt attgacatcc acaaaatcaa
     1561 tatggaaacc atttttaaat agaatttcac ctataaaacc acgaccgata ttaccggcac
     1621 caaaatgaac agaatgtttc attcaatttc tccttttcct atttgaggga catatatact
     1681 agatgacaaa tgacgaaatt caattattca acagcatcaa ataagcgcaa tacttcctct
     1741 tttgactgag catctgctag tttaagaaca ttatccacat ctgcacagaa gatagaaatt
     1801 ttctgaataa tttctaagtg ttcattacca ataccagcaa taccaaaaag aaccgttgcc
     1861 acttgagggt tagatacatt cccaaaatca acccctctag ggacttgaac gactgtaata
     1921 ccagacttga ggacatcatt ttttgctgca tctgttccat gcggtatggc gataaagtta
     1981 cccatgtaaa cagatagctc tttatctcgc tcaatcatgg cttcaatata gtcttcatta
     2041 acatatccac cctcataaag aacttgccca caataacaaa tagcttcctc cttgttagaa
     2101 aattgtttat taagcttaat caaatgtttt tcaagtttca tattagttct ccaatttctt
     2161 aattttttcg ttaaaaatag tatttagcat ctgataaata atggattgat ttcccgtctt
     2221 gtaaatctct gtataaagat gattttcaat aatcgactga ctaatagctg tcattaaatc
     2281 tctagcttcc tcgctagctt ctttagacat tagcattagc agacaccttt tgacctcttc
     2341 ttcctctcgt ttcattgaca atgcggagat aggtagtttg agttcaaaca tagtaaaact
     2401 atttgttgtt actttgctag actgactatg taaaagcacc agaccagtat taggaatagc
     2461 cataggacta ttctggaagt gagataacaa ttttcttgtt aggtaatctc tgtcactcac
     2521 attcttctga tattggatga tttgttctac cgtgtgctca aatgattgat tattctccaa
     2581 ttgaaccaac tcgaattgac tcaaaagctg actactagat attaaataat cttgcaagtc
     2641 ataacttttc tctggagcga tagcatcacg cgcgacaatt gtacgatgtg cctgtacata
     2701 ctgaagtcgt tcctgtaatt caagcaattc tttgacggtt ggaaaactag aaattacacc
     2761 gatttcctga ttagtcagcg gcttggtaga taaaatataa tcatactgac tgagatcaat
     2821 actgtggtaa tctactagag actgaatatc tatcaactct acaaaaggag caacactctt
     2881 aattttactg actaataaat ctctagtgac ccgacgttca tccgttaaaa gcaaaacacg
     2941 gactggataa atctctggac tacggcctaa gctagagata aaatgtaggg caatcatgcc
     3001 atactcatac tctgtatgaa gatatttcgg aaaaatatca ttcactaaaa gactgatgac
     3061 tgtataaaga aatttattcc tttcaactaa aatctggaca gattctggca aattttcacc
     3121 ctgaaaaagg atagggacgc ctaaactgag ccgaatatga tggaaaagaa aattgaataa
     3181 aaccttgtcc ttaaaaaagt caatcttggt atacatggaa accgtatcaa tcagatttga
     3241 aatattgtag aaaaattcac catcaaattt ctccgtaaag agcggattgt cctgacgttt
     3301 aatgattaat tcatccaaga tgctcgcaaa atagataatt tcctgaatac tataaaattg
     3361 tgcagtctgc ttagagtaag tttgaaaaat tttttgagaa atttccaaag cctgcttact
     3421 agtattaggt gaattttcaa tgttttgctc ctgacctata agagataaca agatcgcaaa
     3481 aaacatcttc atcctagcat ccatatctgg aaaacctgac agttgcttat taacaatctg
     3541 actggccagc ctagttctat ctgcttctaa aatatcaaag ctcccaaaat taccggttga
     3601 aaaatctgct actgagatac agtttgtcag taaaatagcc aaaaatcttc tcttaccaac
     3661 tgaatcacca gaaatccgat aacctttttg tcgttcaatt ttcagatcaa agtctaaaag
     3721 acgcttatca atatctgaaa tatcctgaat aatagttaca ttactgactt tcgtgcactc
     3781 ttgcaatgct tcattggtga caaaaccgct ctcagtcaga aggcgatagg taatcaactc
     3841 ttgtctttct tggggactat actcaactaa cacttcaagt tctgtcggca aatcatccaa
     3901 ctctccagtc aaaatatagt atttcccatg cttcgttatt tcgattccat attgctccat
     3961 gctatctgtc aaatctgata aagttcgata aattgttcta gatgaaacct gtaagatttc
     4021 agtcatatct tgcattgaaa gcttccctac atgtaggaaa gccttcaata attgttcttc
     4081 tcgtttggtt aatagcattc gtaatttaga aacctctagt tctatttgta cattctagca
     4141 gccattttgt catattctgg tgttgttact aaactatcca caataagaaa ttgagaattc
     4201 ggtgctgctt gctgcacttc tgcctgtaaa gaaatagttg ttacaatcat tatgttttta
     4261 gaattaaatt cacctaattc tgaaattttg gcagtagaaa ctggaatacg aatattcttg
     4321 tttctaaaga tagcccgaat cgtttcacag cccatagttg cagttccctg tgctttacca
     4381 taagcaacta ctacagcatc aatatggtta aggtcaattt cctgactatc tactggtgct
     4441 gaagttggta tatctccttc aatttctgct attggaaaag ctcctgttaa tgaagctaca
     4501 atttcatcat aacgagggga cgctaagaaa ttatcaacag aaacatgaat agcacttgga
     4561 ctcttgtctt tagctcttgg tgtcagttct tcctgagtaa caattaatgt ttttggtgta
     4621 tcaagcaaat ttgagattgc ctgattagat actggaatct ctagacctgc ttttttaacc
     4681 ttatctcgaa gaatactagc tcccatagca gagcttccca taccagcatc gcaggcgaaa
     4741 atgatttttt ccactgagtc tgtcgaaaca actgcatcaa cagaagttga tactaactga
     4801 cctttagctg cttgggtagc cgcctgagca gcttcgagcg aatcctcagt tgacttgtct
     4861 gcatgaagaa taagggctgc tacaaggaaa gaaacaactg ctgccactaa aacacctaaa
     4921 agaacattta gatgaggcca aacacctttt ggcgccgtag ccataatcgc aataatagaa
     4981 cctggtgaag ctggagattt aagaccagcg tctaagagtt gaaaagtaaa agttccagag
     5041 atacctcctg ccatagcagc taaaaataga gtaggcttca tcataacata aggaaagtaa
     5101 atttcatgaa tccctccgaa gaaatgaata accattgccc cccaagaaga agatttagca
     5161 gaacctttac cgaatacagc ataagctaat agaattccca ggcctggtcc aggattagct
     5221 tccaatagga agagaattga cttaccagct tgagctacct gttctactcc cagaggagta
     5281 aaaatgccat gattgagggc attattgagg aaaaggactt tagccggttc gatgataata
     5341 ttagccatag gaaggaggcg agcattgaca atagcctcaa caccattccc aacagctcca
     5401 gtaagagtcg atacgactgg accgattgcg tagaaagcca aaagcaataa tgcaaaacca
     5461 acgagaccag ctgagaagtt attaactaac atttcaaatc cgggacgaat tttttcttgg
     5521 aacttctcat caaatttctt gatagtccat cctcccagtg ggcccattac catagctccg
     5581 ataaacatag gaacactaga acctgtgatt gcaccaacag tagcaatagc tcctacaacg
     5641 gcaccacgtt ggccatggat catatatcca cctgtgtaac caatcaggat tggcaataaa
     5701 tacgttaaca taggaccaac aacagtagct aactgttcat ttggcagata gccatcagcg
     5761 ataaagaggg cagtcaatac tccccaagca ataaatgctc caatattggg cataaccata
     5821 tttgaaagcg atgtccctag tttttgaacc ctgactttca atgatacttt ttcttccatt
     5881 agatcatcct ccttttctta aaactagtat atacaaagtt aaaaaccttt aaaactaaag
     5941 ttttgtcaca gattttgtgt caaaacatta cttttgacga ggctgggcaa aaaggcttaa
     6001 aaattataaa acgcatcata tcaggtgtac atgaactaca ccccaaaagt tagacagaaa
     6061 aaatctaact tttggggtgt ttttattatg aaattaactt atgatgataa agttcagatc
     6121 tatgaactta gaaaacaagg atatagctta gagaagcttt caaataaatt tgggataaat
     6181 aattctaatc ttaggtatat gattaaattg attgatcgtt acggaataga gttcgtcaaa
     6241 aaagggaaaa atcgttacta ttctcctgat ttaaaacaag aaatgattaa taaagtctta
     6301 tatgaaggct ggactaaaga tagagtttct cttgaatacg gcctcccaag tcgtacgata
     6361 cttcttaact ggctagcaca atacaggaaa aacgggtata ctattgttga gaaaacaaga
     6421 gggagagtac ctaaaatgag acgtaagcca aaaaagagac ctgaagagag gacagaatta
     6481 gaacgtcttc aagcagaaaa tgagtacctg agagcggaga aggccatcct aaaaaagtta
     6541 agagaactcc gattgaagga ggaaaaagag aaagaagaaa gatagaaatt gttcaagaat
     6601 taatgactga gttttcgtta gatcttcttc taaaagccat taaactagct cgttggacct
     6661 actactatca cttgaaacag ctagacaaac cagataagga ccaagagctt aaagctgaaa
     6721 ttcaatccat ttttatcgaa cacaagggaa attatactta tcgccgggtt catttagaac
     6781 taagaaatcg tggttatctg gtaaatcata aaagagttca aggcttgatg aaagtactca
     6841 atttacaagc taaaatgcga cagaaacgaa aataaaggag acgttggtaa gaaggcagag
     6901 aatctcattc aaggccaatt tgaaggctct aaaacaatgg aaaagtgcta cacagatgtg
     6961 acagaatttg ccattccagc aagtactcaa aagctttact tatcaccagt tttagatggc
     7021 tttaacagtg aaattatcgc ctataatctt tcaacttcac ccaacttaga acaagtacaa
     7081 acaatgttgg aacaggcatt cacagagaag cactatggtg gagtccttct ttgggattct
     7141 gaaatcggaa atgttttatg gctatgagaa aacatttaaa tcacttaatc aattggaaca
     7201 agctattata gactatattg attattacaa caacaagaga attaaggtaa aactaaaagg
     7261 acttagtcct gtgcagtaca gaactaaatc ctttggataa attatttgtc taacttttgg
     7321 ggggcagtac agaaaaacct tgatactatg cgttttttga ttgtgaagac ttttatccac
     7381 ctattataca aagttaagta tccatcacag tttacaatta gccttcatta cttaacccat
     7441 tcaccattat agtttaagta gtaaccacct actgtggtat taacagctaa agcacctgaa
     7501 tcataggcat agtaccagtt accattgact tggaaccagc ctgttttcat atctccatta
     7561 cttgcattta gatagtacca agtcgaacct tcctgatacc aaccagttgc catagctcct
     7621 gatgaacgga gatagtacca cttgttacca aggtattgcc agccagttct catatcacca
     7681 ttttcagcat ctagatagta ccaagttgag ccttccttat accagccagt tgccatagct
     7741 cctgacgaat ggaggtagta ccatctatta cctagaaatt gccaaccagt ttgcataatg
     7801 ccagttgttg gatctagata gtaccatgga gctgctttta gcttctcttc atcataggta
     7861 agaggggaat ccttaaccca accacgtgct agctctccaa ccgccaatct gttgatgcgc
     7921 gaatcaaagc caccatcctt ctgtaaataa taccaatgac cctcttcgta aatccaacca
     7981 gtttttaaag tatgataact acgctgatct tcaaaataat agactcattt ctctgctggg
     8041 ctatcatatt cttccccatg atgtttgttg gtattcgtag cagtttttgc ttctaaaact
     8101 tgcttgccaa caaattcttg taatacacca tcttgaccaa aataaaacca atctggtcta
     8161 agagaaatct ctattcttgg agaaggacca atcgtaaccc ccttgtgtgg agcaggtata
     8221 tattgccagc cgacaaccat ctctccggat aaaggatcaa aataatagta cttcccatct
     8281 atcactctcc agtaggtttc ttttagatcc ccctttttgt agtaggttct accattttct
     8341 tggacaaact gccatccttc agaatcatct gcaaatactg tactggtccc tagcaaaccg
     8401 aagaaaaatg tggctagtgc gacttgcatc atttttttca aaagcttcat tttatatatc
     8461 ctccaacatt aaatccactc accagatgaa gcgaagttat aaactttacc atcgatagtt
     8521 tggctacctg taaccattgc tccatttgaa ttgagataat accattttcc atttactttt
     8581 tgccaatctg taagcatctt accggaactg tctaaataat accaagtatc atttatctcc
     8641 tgccaacccg tttgtttatc atgttgtcta taatggtacc aactaccatt tattttcttc
     8701 catccttcaa gagagtaacc tttaagaacc gaataaataa atggcaaatt tcgttcattt
     8761 aatttaactg cactgttaat ttgattagct ccatctccta aagtatgcac tcctactaca
     8821 cggtgactag catcataaac tgtagatcca ctagacccct ctaaagtatc aacttggtaa
     8881 tccaagaaca tgccatcatc acttaaaact tgttttttat ctgtatacat ttgatgaatt
     8941 ttaaaattat atgatggata gcctgtgata gtcacagtta ttcctgtcaa atttttttga
     9001 ctagtaggaa gacccaaagt ccctaatttt gcaccaatgg gctcttctag aattaataaa
     9061 gccaagtcat attcccttgc atccttagaa tttaaatttc taaattcctt caaataacga
     9121 acttccttta ctttgatctt tccaaatggt tcttgacttg gactaacagc cggaagaaca
     9181 taaatatcat ccgcttcttt cccatagtca tgtctgtagt agttgtgagc cgctgtaatt
     9241 aacacattat ctttaataaa attcgcactt cccctccctt ctacattcat tccatcggct
     9301 actccataat acttagataa aaccatagct gaagtcgaat acggatactg taaagtatta
     9361 tcaattttaa tcaaatcatc attaccgata atacttctga ttgcttttgg tagtatgaac
     9421 catatgttgg tgaaatctca gataatgaag aatcattaga tcctggacct ttttctagtg
     9481 tctcacttac ctcatattct tcacccgtac tagaaataac actcaaagca gatactgtcg
     9541 ataactggct agccaataaa gtactcgcaa taattgaaat acccaatttt ttataaacag
     9601 ttttcttcat tattgtagcc tcctaatgta attatagcgt actattctaa atttcttaat
     9661 ctactataga atcaagaaat ctaccacctt ctttaaatac cctccattat cacataaaca
     9721 ggtaaacttt tcaattaatg actgcgcttt tcaatcacgc tagaggtact tgcttgcttc
     9781 tttgatacta agttcagcca ttctttcctt atttttctca ataaagcatg ttacccaagt
     9841 gggattcgtt ttggagtagt ctcgcagagt ccagccaatg gctttattga taaaaaattc
     9901 tgtttggttc aagttatgaa ggagaatctt ttccattaat tgagtattgg tcttctcttt
     9961 tcttaacaac tggtggttaa tagcgacacg tctcagccag atattatctg ataggctcca
    10021 ttttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcagat tgctcaaaac
    10081 actgttttaa ggttgcagat agagctgacg tggtttgaag agattttcga agagtattaa
    10141 gattatttct tctagctcag ggtgttcata caccaaactc cctactactc gatctaggat
    10201 atctaccgtg tcccaccagg attttgtcac gactaactgc tctagcttag gcaaatcggt
    10261 ttcctttaga taagactgca ttgctttcaa atagttagca gccacatatt ggtattttct
    10321 aggctccttt tcccagcaag tgtctgcaaa atcccaatcg ataatctttg tttttttcgc
    10381 ttctggaaaa tatttttata gagtttattt ctttcaacct agaaaagaaa attgatggca
    10441 catataggct tccatgggcc ttgctttttt agagtctttt gctgcttcta gctcctcaag
    10501 taaatctgct aaactcatct aaaactcctc atgcccgacc aaatggtgct gaaaggcata
    10561 gacagccgcc tgggtacgat cgctaacttc aagtttggca agaatgttgg acacgtgggt
    10621 cttgaccgtc ttgagagaga taaagaggtc atctgcgatg cgctgatttt cgtagccctt
    10681 ggcgatgagt tggagaacat ctcgctcacg cgcagtcaat tcttcatgaa gttccatatg
    10741 attgcggtgg tattcaacct tcttgctaac ctcttgctca atggccagct cgccagcagc
    10801 taccttactg acggcatgaa gcaattcatc tgcactagaa gtcttgagca tatagccttt
    10861 ggcaccagca tctaagactg gcatgatttt ttcattgtcc aaataagagg tcacaatcaa
    10921 aatcttggct tcaggccatt ctttaaggat tgctaaggtt gcgtcaatcc cattcatctc
    10981 aggcatgaca atatccatga caatgacatc tggacgcagt tccaaggcca agtcaatccc
    11041 ttgagacccg ttggacgcct cacccacaac ttctacatcg tcttggaggt caaagtagct
    11101 tttcaagccc aatcggacca tttcatggtc atctactagt aaaattttca tctttactcc
    11161 tttatcattc cttatctaac aggggaatac ggatatcaac cgccagccct tgcttgggag
    11221 ctgtcaagag ttgaactgtt ccagccatat cttcaatccg ctccttgata tttcgcagtc
    11281 cataactcaa gtcgtctaag ctccctaact ggaaaccaat cccattgtcc accaccttca
    11341 gttgcaattc aacatctgtc tgatagaggt agacatctag gcaagatgcc tgggcatggc
    11401 ggagcgtatt gctaatcaac tcttgcagga tacggaagat atgctcctcg attttcttag
    11461 gaaatttcgt catattctgc ttgagactaa ccctaagatc actcttgtcc tcaagctctt
    11521 ttaagagaat ttgaatccct tctatcaagc tcttctgctc cagttcaact ggtcgcaaat
    11581 gcaagagcaa aacccgcaaa tccttctggg ctgtttctaa aatagctgtg acactctgca
    11641 actgggtctg catcttttct ctatccaatt tcaaagcctg ctgactgata cccgataaaa
    11701 tcatgtgggc cgcaaacaac tcctgactga ctgtatcgtg caaatcccga gcaattcgct
    11761 tccgttcctt ctcgatgatt tcctcttcct gagcaaggct atgattttca gctttttgaa
    11821 gagcctctgt caaaaggtta agtttacctg ataaggactt gaaactggca tccaaatctg
    11881 gatctgcaac ctgaaccact tcttgccctg ccaataaacg cttgagatta gcctgcattt
    11941 ttcttagaga aagctcttcg atccctcgcc aaaacagggc taagagacag gttatggaca
    12001 tgctgaaaac caacaataaa aagacaaatt tttctgtttt ttcgacatcg tgcaaaaaga
    12061 tagaccagtc aaaatcaagt atttccagca agctgtggga gaaaaaaaag acaaacagga
    12121 aggaggtgag agcaataatg acataggctt gttttttcat cctctaacca cctccacatc
    12181 accaatcata gtggtcaaga aaatcttgac actcttgtta ctcttgagat agtcttttgt
    12241 ttcttgatga tagtgttcat tgcggagggc tcgcttgggc tggttgaaaa aagtcaaatc
    12301 cccatagaga cagttaacgc tgagactgac ttccacatct acaggtacga tgattttggt
    12361 cgttcctacc atctttctga ggataatgac attgtcatga ttggttaaga tgaccctctc
    12421 cagatgaata gtgtccttgc ccatgaagcg aaagagattg atatcatcga attggcaagt
    12481 ctggtagctt gaaaaatgat gaagatttcc aaaccaacga tttttctcct tcttaaccgt
    12541 cacgacctct tcaaaaacca aattggtctg ctctttttcc tggttcatca tcgggtaaag
    12601 aagaaagagg ctatagataa ccgcaacaaa aatagctaga atcacaaaag gattgagcat
    12661 aacgatgaaa aagaagagaa tggttgccgc tactaaaaga agattatttc cctctttacc
    12721 agtgtagtag cgaatcaaaa gcaaaaagag gaatagtatc agcagaaaac gcgaaaaatg
    12781 ctctgatacc atcaaaatca gagctcctgt cagaagacag gcttcgataa ataaaaagat
    12841 tttaaatttt ctcataggtt catcctctcc cttctatttt atcacaattc aaaaaagtca
    12901 cctcagtctg aggatggaaa aaaggcgctg gttacgcctt tttcatctga tcctttgctt
    12961 cttttaattt tccataaaga agatagtcta ctttttgtag atctgctatg gtggcacagt
    13021 taagggaaca cataatcaag cgtaggtctg ctttccagcc ttggacaatg ccaatcactt
    13081 cttcaactgt gtaggtttca accaattcca gaacggttcg tgacaatccc acagacttag
    13141 caccaaaaac caagcactta atcatatcca gcggattccg aacccctcca ctaaccaaga
    13201 gttcgacctt atctttccat tcttgggcat tgagaagggc ctgcatggta gactgacccc
    13261 attgattgag gtaatcacgc tggccactac gacggttttc gatataggca aagctggtgc
    13321 caccacgacc cgataggtcc actgtacgaa caccgaattc ataggctctt tcgattgtct
    13381 tggcatccat tccaaagccc acttccttga ggacaatagg aacgggaatt tgctcgctat
    13441 aatctgctag atgcgattgc cagcttctaa acttcctttc tccctcgggc atgagtaatt
    13501 cctgcatgac attgacatgc acttgcaata gaacaggatt catctcttct acagtctgaa
    13561 gtcctaactc gacaggcttg tccaatccaa tattggttcc aaggaggaga ttgggatgac
    13621 tagacttgac agaaaaagaa tcatccgttg gatttttgag ggctgcgcta taagaacccg
    13681 ttacaaataa aataccacag gattccgcca cctgagccag cttttgattg atttctcttc
    13741 ccttattact tccaccagtc atggcattga tataaaaagg aaagtcccac tttcgaccag
    13801 caaactctgt cgaaagatcg atttcatcca gattgtaaag aggcaaggaa gaatgaatca
    13861 gctccacctc atcaaagcta ttataggaac ttttctgctc aagggcatag aggatatgct
    13921 cgtccttacg atttgtcgtc atgtcctatc ctttcttgat ataagagctc aatccccaga
    13981 tcggcccaac gattttttaa ggttttggtt gattgcgcat caaaactcaa ggcgatgcca
    14041 cagtcaccac caccagcacc actactcttg gcaacggcct gcaaatcttg actggcttct
    14101 ttcaactgtc taagcaaagg cgtgtaaata tctgtactca agccttctaa aagcttgctg
    14161 gctacttcta cttgctcgat aactttttcg gctttcccct gctccaaggc ttcgaccaaa
    14221 gaaaccaccg tttcttttga ggaacttaaa aaattttgat tgatattttg cttgatttgc
    14281 tggaccatgt gactcgatac agccacttcc ttggtccatc ccactaagaa atcacattct
    14341 aaagttggtt tcacttgtga gataaaaaat ccccaatcac gctccagaac tgtcgccaag
    14401 ttttcttctt ctaaccaagc agccaccttc tggcgatcaa atgactggta gagaaccaaa
    14461 tcctctgcca caatacaggc aaggtcgccc atggaaccat tgtctcctcg cttgagcaag
    14521 acagcgctag tcagcttgaa caagagattc tgatcaaccg aaagattata gagagccagt
    14581 aaagccttga caaccaagac aacgacgctg ccactagaac ctagaccaaa ctttttccct
    14641 tctcgttcca ttttgccaca gatttttagg gaaaaaggtc ttaaattctg accgcgaaca
    14701 gcgaggaagt ctcccatcaa agcaatcgtt tcttgaatca agctgtagtc aggattaggc
    14761 cttaagtcca ctgcgaaatc aaacatatct gaatagatac ggtagctgtc agaaaaagca
    14821 atttcagccc tcatatagat gggaatatcc tttatcaaag ctaactgccc tggctctaaa
    14881 atagcatatt cacctgccca atagagtttt ccgcaagttt taacagcaat catcttgact
    14941 caaatccttt gtttttgaca caatcaagcg ataacgctga ccgaaaattt ctgataaatg
    15001 ctccaagtct ttctcctgac agaagacctt aacattggga ccagcatcca tggtaaagta
    15061 gcaggcctct cctttctcac gaagctggcg aacaaagtcc atagcctcat aagaggcatc
    15121 cgtcagataa gaaaaggctg gactagcagt ctttgtcgta gcatgcatag ccagggcatt
    15181 tttctccgtt aattctccaa tcttggcaaa atcattttcc ttgagataaa tcagcatatc
    15241 ctgatagtcc ttctcagact gacgaaccca gtcgtcgaaa gtcgtcgagg tttccacaca
    15301 aagtttcatc ccgtcacggc tagagattgg ttttttcttg tcctctagca ccaacataat
    15361 catagctagt ttcaagtctg tctctacagg gtaaatttct ccactatcct tatcccaggc
    15421 tcctagtggt ccataaaaac tccgagaaga agagcctgag gcaaacttgg cttcctgcgc
    15481 taactgactt ctatccaatc caagcttgaa ataagcatta caagccttga ccagggcgga
    15541 caaaccacta gaacttgagg acaggcccgc tgccgtaggc atattgtttt gagtatcgat
    15601 acggacaaag ccctcaccag ctggacgata acggtcaata atcttactca tcttggcatg
    15661 ctcgacctca ttttgtagct gaccattgat gtaaaattcg tcagctgtta cattggctgg
    15721 taaaggcgac aaggtcgtct ctgtatacat attttccaaa gttagagaaa tactgctagt
    15781 agcaggcacc atctcttttt cttttttctt tccccaatat ttgataatag caatatttgc
    15841 gtaggaacgt actgttacag gctctctatc catgtctgaa cagctccttt ctcttctaat
    15901 ctttctgcta gttcttgtgc gtgtgtcaaa ttggttacca aggctatgat acaacctcct
    15961 agcccaccac cgctcatctt ggcacccaga gcaccatggc taagagtcgt ttcaaccaaa
    16021 aagtctgcct cagggctact gactccaatt tcttttaaat gtaaatgcgc ttgactgagg
    16081 atttgtccca gtccttcagc atctttttgt gaaatcgcaa cttctgcttg ctgggttaat
    16141 tctcccaagg catgcaaaaa cggtagggca tccttgccct tattttgaac cacttggatg
    16201 gcttcacgag tatgaccata aacacccgta tcggcaatca ccaaataggc ggataaatcc
    16261 atctcaagtt ctgtaaatcc tacgttcttg ataaagcgaa taggttggtc actgagacag
    16321 gtcttagcat ccaaaccact aggattcata tgggcaatca tttcagctcg attgaccaag
    16381 atttctagta catcatgagg cagatcagcc tgatagtagt caaataccgc acgaatggcc
    16441 gctatgctga tagccgctga cgaacccatc ccccgtttct cagggatagc cgagtcaatc
    16501 tcacaacgaa tgcaggcttc tgtgatatcc aaatactcca gtgaggcata aaccgccatg
    16561 gacaaggtat cctcctcata aaggcgccaa ggactctctg caggaactac cttacaggtc
    16621 acctccacct ccaaaagagg cagggaaatg gcaggataac cgtaaacgac cgcatgttcc
    16681 cctattaaaa ttatcttact atgtgcctga ccgacaccaa ctttttttgt cattttttcc
    16741 ttttactaga cgaaaaaacg tcttattttt catacaagta ttaattcttt cctatctatt
    16801 ttattatatt ttcacaaaaa aagcgattgt ttccattcac aatcgcttct ttcattaccg
    16861 aacccattca ccattataat tgacagaata gccatctaca gtcgtattca ctgccaaggc
    16921 acctgagcta taagcgtagt accatctgcc attgacctgg aaccaacctg tcgtcataga
    16981 acgatgaaag aaactccata ccattaagta aagaggaaag tcatgaggga gcatgcgcca
    17041 ttgacaacct gttttagtga cgtacaaagt ttcattaaca agtactcgtt tcggccattt
    17101 ataggtgcgg tgtttgcaga aatagggttc aatcttcgcc cattcttgat cgtttaaatc
    17161 agtatcatat gctttgcgtg tcataactct agtttaacat ttttttgtga atacaggttc
    17221 taaataatcg accacgaaaa tttcttaagt ggaaaacgcc ttatgaagta tgctacggga
    17281 aacttatgca cttaatttga caactcaata tgtaaaaata tatactatat tagattgaaa
    17341 ctagaatagt acacctctac ttctaaaaca ttgttagaaa tcgattt
//
LOCUS       DAVKWS010000039        17323 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKWS010000039 DAVKWS010000000
VERSION     DAVKWS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.039"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(1..249)
                     /locus_tag="VW852_001456"
                     /pseudo
     CDS             complement(1..249)
                     /locus_tag="VW852_001456"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            complement(282..620)
                     /locus_tag="VW852_001457"
     CDS             complement(282..620)
                     /locus_tag="VW852_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272584.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEYYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKKLIK"
     gene            complement(633..1406)
                     /locus_tag="VW852_001458"
     CDS             complement(633..1406)
                     /locus_tag="VW852_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0272585.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(1852..2607)
                     /locus_tag="VW852_001459"
                     /pseudo
     CDS             complement(1852..2607)
                     /locus_tag="VW852_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2745..4367)
                     /gene="groL"
                     /locus_tag="VW852_001460"
     CDS             complement(2745..4367)
                     /gene="groL"
                     /locus_tag="VW852_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV0272586.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(4383..4667)
                     /gene="groES"
                     /locus_tag="VW852_001461"
     CDS             complement(4383..4667)
                     /gene="groES"
                     /locus_tag="VW852_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV0272587.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(4937..5332)
                     /locus_tag="VW852_001462"
     CDS             complement(4937..5332)
                     /locus_tag="VW852_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV0272588.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(5410..6171)
                     /locus_tag="VW852_001463"
     CDS             complement(5410..6171)
                     /locus_tag="VW852_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV0272589.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(6204..6830)
                     /locus_tag="VW852_001464"
     CDS             complement(6204..6830)
                     /locus_tag="VW852_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV0272590.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(6846..7163)
                     /locus_tag="VW852_001465"
     CDS             complement(6846..7163)
                     /locus_tag="VW852_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV0272591.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(7160..7459)
                     /locus_tag="VW852_001466"
     CDS             complement(7160..7459)
                     /locus_tag="VW852_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV0272592.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7744..7935)
                     /locus_tag="VW852_001467"
     CDS             complement(7744..7935)
                     /locus_tag="VW852_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV0272593.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(8096..8770)
                     /locus_tag="VW852_001468"
     CDS             complement(8096..8770)
                     /locus_tag="VW852_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272594.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIHGDVAFGKL
                     EIVYAK"
     gene            complement(8776..9222)
                     /locus_tag="VW852_001469"
     CDS             complement(8776..9222)
                     /locus_tag="VW852_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272595.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(9336..9839)
                     /locus_tag="VW852_001470"
     CDS             complement(9336..9839)
                     /locus_tag="VW852_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0272596.1"
                     /translation="MKNYQEWYRNIRSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(9836..10198)
                     /locus_tag="VW852_001471"
     CDS             complement(9836..10198)
                     /locus_tag="VW852_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV0272597.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(10264..12123)
                     /locus_tag="VW852_001472"
                     /pseudo
     CDS             complement(10264..12123)
                     /locus_tag="VW852_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(12162..12611)
                     /locus_tag="VW852_001473"
                     /pseudo
     CDS             complement(12162..12611)
                     /locus_tag="VW852_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(12735..13451)
                     /locus_tag="VW852_001474"
     CDS             complement(12735..13451)
                     /locus_tag="VW852_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0272598.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(14332..15747)
                     /gene="ply"
                     /locus_tag="VW852_001475"
     CDS             complement(14332..15747)
                     /gene="ply"
                     /locus_tag="VW852_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV0272599.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(15758..16090)
                     /locus_tag="VW852_001476"
     CDS             complement(15758..16090)
                     /locus_tag="VW852_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272600.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(16161..16769)
                     /locus_tag="VW852_001477"
     CDS             complement(16161..16769)
                     /locus_tag="VW852_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272601.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(16782..17240)
                     /locus_tag="VW852_001478"
     CDS             complement(16782..17240)
                     /locus_tag="VW852_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV0272602.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         5097 a         3916 c         3023 g         5287 t
ORIGIN      
        1 attttcaagg caacagaacc aaaaatttaa acaagctgac acaactaaat ttctattctt
       61 gccgattttt tcgtaataat gtgtacgctc tagtatttct tttccgacca tgtaaatata
      121 atctatttca aaaagatgat ataaattacc aatcagcgtt atctcatagg actcccatcg
      181 ttccttctgg aataaatagt catatactaa atttagatca tctctactaa ctacatgatt
      241 acaatctagc tgatttcata atccctgaac cataattgta tttattttat gagttttttc
      301 ccactcttct caaaaagttt ttgttgctta ttttttatta cctccaatcc tttaatattc
      361 aaactataaa aatttggaga taaattaacc aaaaaatcat cacgctccga tcttttataa
      421 tattctaagt gattacaaaa attctctatc gaaacattga tatttgataa taattcaaaa
      481 aatgttgaaa gagaaatttc attttctccc ctttcaaatc tagaaagttg agattttgat
      541 ataaaatcag ctgcagcata ctctaaagaa tatccgttta attttctaaa atatcgaaaa
      601 gcttctccgt aatttttcac actttcattc ccttaacttt cttttcgagc gataaacata
      661 ccatattctt taggaatgat caacatacca ttattaaact cactttccaa tcttttacgt
      721 attatttcct cttctagtga acctatttct gaaattcctt taaacgattg gatgtattta
      781 actaggtcat ctatactagt tacctgtaac tcatcatcat agagtaatgt atcgacagag
      841 ttaaaatacc tactcaggta ccttttacca ttttgtaaag taaacgttct attttctaaa
      901 tcttgattaa cctcatcttt aaataaactt gccaaataat ttacaactcc attttcacca
      961 aatgtagcac agtaaaaaat gcctccggtt tttaagaccc tattcacctc agagagagct
     1021 ttaggaatat cattcacatg atgtagaagc atattagcaa taacaatatc aaaagtttca
     1081 ttctcaaaag aaatcttttg aatatccatt atctcataat tgacattgtt tctatttcca
     1141 atcactgact tcgttgattt taccatatct ttagaaaaat ctgtaacaat caactgcttc
     1201 attttatcta tagaatcact attacttttc cacaattctc ctgtaccaca tcccaattct
     1261 agaaccttta cttcatcagt gatttggtag ttagaaaata tccaattatt aaatcccaat
     1321 ttattttttg aatacttctt atgcaattct actcgaatat caagattatc atgactttta
     1381 tattgttcaa ttactttatc aatcatttta aatgacctcc actctataat ttggcattat
     1441 atcacaagaa taaagatagt gttaaataag ttatataaat gcaaaaaagg aatattccgt
     1501 tatagcagag ttgtaacaca tttctataaa gaaatttatt cctacgactc agtttggcat
     1561 gcttaacttc ctaactcaaa aacaaagcat cgattaattt atccgttctt cttttgaaac
     1621 agatttgaac tgataattgc tcgttttaat ttaaaaagct cttttgagca aaacttcatt
     1681 tttttaagag tccaacaatt tctgtctatc gcttttctac tagcaaaaaa cctaccttga
     1741 ggcaagtttt catttatata tctaatttcg taacagtata gtaaaatgaa ataaggacag
     1801 tcaaatcgat ttctaacaat gttttagaag taaaggtgta ctattctagt ttcaatctac
     1861 tataaatttg atatgagaat agtctttctc aacatcctta tccaacaagc atgctgtggc
     1921 gtccttctta acttaaacca ggtcaatatt ctgggcttgg gctgcataga cgcagccaca
     1981 ataacattga cgatagatat catactcctc acacatctct actgaacgtt tgtagccttg
     2041 atttttcttg aaatcgttgg gaagatagtg gggcgtgtaa attttttgca catcgattcc
     2101 gatgctattg atagtttgag aattcttatg aggactgatg gtcaaggctg aaccaaagta
     2161 gtcaaagccc aagtccatag ccacttgcgc tgttttatcc agtcggtagt caaaacaaac
     2221 cttgcaacgg tcgccacctt cgggctcctc ctctagcccc ctaactagtt ttcggtatta
     2281 attgggttcg tagggagctt ctaggtactg aaccgtattt cctgtccgct cattaaaatc
     2341 actaacaaat ttcttggtga catagacccg cttatggtat tctgccttgg gatggatatt
     2401 agaattggca aaatagatgg tcacatctgc atacttggtc aaatattcta gtgtataggt
     2461 actacaaggg gcacagcaaa catgcacgag aatggttggc cgctactcat ttttttccca
     2521 tgcttgtacc attttctgca tgacacggtc ataattaatc ttctgattgg gggtcatctt
     2581 gcacagaatt tcttctacat cgatcatgtt cttctccttt ttctagtctt attttatcat
     2641 ataagttagg agagctcaaa tcaatttaga agtgaatatt ataaaaagaa gggttagtca
     2701 ttccctcctt ttgtgttttt tataagttgt tttctataga aagcttacat catcccgccc
     2761 atcatacttg gatccattgc tggagctggg gctactggtt ctggtttatt ggctacgact
     2821 gcttctgttg tcaaaatcaa gctggctaca gatgctgcat tttgtagggc tgaacgactc
     2881 actttaactg gatcaatgat accttgatca atcatgttaa cccactcgcc agttgctgcg
     2941 ttgaatccta taccaagctc agcatttttc aaacgatcga taacgataga tccttcaaat
     3001 cctgcattgt gagcaatttg acgaacaggt tcttccaaag cacggagaac aatattacgt
     3061 cctgttgctt catctcctgt caattccaag gtagcaacag ctggaatcac attggcaaga
     3121 gctgttccac cacctgcaac aataccttct tcaacagctg cacgagtagc gttgagggca
     3181 tcttcaatgc ggagtttcat ttctttcaac tcagtttcag ttgcggctcc gaccttaata
     3241 accgctacac cacctgacaa tttggccaag cgttcttgca atttttcacg gtcaaattca
     3301 gaagttgtag tttcgatttg agacttgata accgcaacac ggtgagaaat cgcttcagga
     3361 tttcctgcac cttctacaat aaccgtgcta tctttgtcca cggtcactct cgctgcttga
     3421 ccaagagctt caattgtcgc atctttcaac tcaagaccaa ggtcttctgt gataactgtt
     3481 ccgcctgtta agatggcgat atcttcaagc atggctttgc gacggtcacc aaaaccaggt
     3541 gccttgactg ctactacgtt gaaggttcca cgaatcttgt tcaaaacaag agttggaaga
     3601 gcctcgccat ccacatcatc cgcaataatc aagagtggac gattgctttg gagaatgctt
     3661 tccaaaagtg gcaagatttc ttggatattg gaaattttct tgtctgtaat caaaatgtac
     3721 ggattttcaa ggtcagccac cattttttcg ctatcagtca ccatgtactg tgaaaggtaa
     3781 ccacggtcaa actgcattcc ttctacgact tcaagctctg tttccatacc acgtgactct
     3841 tcgatggtga tgacaccgtc tttgccaact ttttccattg cttcagagat gtactcacca
     3901 actttttcag aacgagaaga tacggctgca acttgagcga tagcttcttt attggcaaca
     3961 gggatggcgt tgtttttcaa agcttcaact gctgcggcaa ctgctgtttc aatcccacga
     4021 cgaataccga ttggatttgc acctgctgtg acatttttga ttccttcacg gacgatagct
     4081 tgggtcaaga ctgttgcagt cgtagtcccg tcacctgcga tatcattggt tttagaagct
     4141 acttctgata ctaacttagc acccatattt tcaaaatggt cttccaattc gatttctttg
     4201 gcaatggtca caccgtcatt ggtaatcaag ggtgaaccga atgacttttc aagaacgaca
     4261 ttgcgacctt ttggtcccaa ggttacttta acagtgtctg caagaatatc gacaccacga
     4321 accatggctg aacgggcatc tgatgaaaat ttaatttctt ttgacatact tactttctcc
     4381 ttctattcct caatgattgc caaaatgtta gcttcgccta cgataatgta cttttcatcg
     4441 ccatctttga catcaagacc tgcgtgggct tcaactaaga cacgatctcc agttttaaca
     4501 cttggagcaa ccaagtcacc gttcaaggta cgaacacctt gtccagtagc cacaacttga
     4561 gctgttttgg ttttttcttg ggctgagcct gcaaggacaa agcccccaac ggtttgttct
     4621 ttttcttcta tttttaagac cacacggtcc cctaatggtt tcaacatctg atttcctcca
     4681 taatgagata gatattatta gcactctttg aactggagtg ctaattcata attctattgt
     4741 atcacttggt cagaaatagt caagaaaaaa gtctgacttt ctcaagataa aaagcctgag
     4801 accaactcag actttttaat gtttatactc aatgaaaatc aaagagcaaa ctaggaagct
     4861 agccgcaggc tgctcaaagc attgctttga tgttgtggat aaagctaacg tggtttgaag
     4921 agattttcga agagtattaa aatggcaatt cttcctcttc caagactaaa tctgccaaat
     4981 cttggcctgc attattttca cgcatggcac gttgagcacg actttccaag agttggaatc
     5041 ctgttacaag tacttcggtc acgtagttca tttggccatt tttctcaaag cgacgggtac
     5101 gcaattctcc atcaacggaa atgagactac ctttggttgc gtagcttgcc aaagtttctg
     5161 ctagtctgcc ccataggacc atattgacaa aatcagcttc acgttcaccg ttttggtctt
     5221 tgtaacgacg gttgacagcg atagttgctc gcgctaccga cttgtcattg ttggttttgt
     5281 gcaattctgg tgtagacgtt aaacgcccaa tcatgataac tttattatac atattttctt
     5341 cctcctactt atctattcgt aggaaatcaa aaaaagttac agaaatttgt aacttttcga
     5401 ggaaatttct tattttttat gaatcatgaa acctgtcgcc tgttgattgg ccataatggt
     5461 catatctgta atctgaacac ggcgaggctg actggtcaca tagactactg tgtctgcaat
     5521 atcctgagct tgcaaggctt ctattccttg gtaaacggac gcagctcgct ctttatcacc
     5581 atgaaaacga acagttgaga aatctgtttc gacaatccca ggctgaatgg ttgtcacctt
     5641 gatatccgtt gcgatggtat caattcgcag tccatccgaa aaggtcttaa ccgcagcctt
     5701 ggtagctgag taaacagcgg caccagcata ggcgtagatt cctgcggttg accccatatt
     5761 gataatatga ccttgattga cttttatcat tgctggcaag aaacagcgag taaccgccat
     5821 cagaccttta acattggtat ccaacatggt caacatatcc aactcctcat agtcttgata
     5881 gggagccaag tcaagagcca gtccggcgtt gttgaccaag atatcaatcc tccctatcgt
     5941 ttctagaata tcagagcaaa cagtcttcac catgaccata tccgtcacat ctagtggaaa
     6001 agtccagacg gtttgatttg gaaagcttac tgcaaactcc gacttgagga tttctaatct
     6061 gtctatccgt cgtcctgtta gaacgacatc ctcaccctgc tccagataag cacgcgcaat
     6121 cgcttcaccg attcctgagg ttgctcccgt aatcactaca ttttttgcca tcttatttcc
     6181 ttctatcaga tgctgacaag ttcctaggca gtccagtgtt tcgctgggtc gaacggtgtt
     6241 ccgacaactt ggtcttctga taattcaagc accccacgtt tttgcggagc atttggcaaa
     6301 tgcaattcac gaggactaca catcatccca aaactctttt caccacgaag ttcgcctggg
     6361 aaaatgaaat tcccttttgg catcatggca ccaggaagag ccacaatggt tttcaaccca
     6421 acacgcgcat taggtgctcc tgcaacgatt tgcactatct tgtcacttgc gactgcaact
     6481 tggcagatgt tgaggtggtc actgtctgga tgagctacca tctcgacaat ttcaccaaca
     6541 acaaacttag gttccttatc attaacaatt tcttctgtaa aaccttccgc ctgtaattct
     6601 tggttcaaac gagcgacttg ctcatctgat aaaaagactt gaccgcgctc tgcaatttca
     6661 aacaagctcg aaacctcaaa aatgttccaa gctactgttt ccccattatc tttgagaaaa
     6721 acacgggcta ccttgccttt gcgttccaca ttcagtttgg catctccgct atttttcacg
     6781 atgaccataa ggacatcacc gacatgttct ttgttatacg taaaaatcat tgtttccttt
     6841 ttctcctatt tcaatcctgc taaaaaatcg ttaatttgct ccttactttt acggtcgcga
     6901 ttgacaaaac gaccgatttc cttgtccttt tctagaacaa caaggctagg aattccgtaa
     6961 acatcccaga gtttggccaa atccatatac tgatctcggt ccattcgaat aaaggtgaac
     7021 tctggattgg tctcttcaat ctctggtaag gcaggataaa tataacgaca atcgccacac
     7081 cagtctgcca caaaaaggaa gaccttcttg cccgcttttt ccactaaaga tgctaattct
     7141 tctaaacttg ctggctgtat cataagactt cctcctcata gactaggtct tcattttcat
     7201 agacaaaggt ataatgacgg ccatcctcaa aaatgacgcc accaaccaag ctctccagac
     7261 tgctttcgta aacttgaaca taaagggtcg caatttcccc catgtcggaa aaatggtctc
     7321 gcacaatctc tgtcaactct tcctgagtct tcatgagctt acggtcatct gcaacttttt
     7381 tcgtagcaag agcaaggctt ccgataccta gcagagccag acctgccatc cacatttttt
     7441 tagctttcat accattcatt ttaacacaaa aaaggcttca ggacaaatga ggaagcagca
     7501 gaaaagcaag taaaaagcct cttcctttaa ggaaaaggac ttcttatact aagagcaaac
     7561 tagaaagcta gccgcaggct gctcaaaaca cggttttgag gttgtagata aggcgacgct
     7621 gacgtggttt gaagagattt tcaaagagta ttattcttat tgccaggcac ctaagttgcc
     7681 aacgtagtaa ccatcaggtg tgtaggtatt gcgaagcatc ttacctgatg aagccagata
     7741 atactacttg ccattgtctt tgacccaatc attcgcaatc atggaaccag aagaacttac
     7801 ataataccat tctcccttgt cataaaccca agtactgact ttcatggttc ctgagcaatt
     7861 aaaggcaaaa aaactgtcca atatcattcg ttttttaaaa gcatttgaca ctacatcatc
     7921 tacgctgtag tacatagata catcaaacgt aaaagccaaa atatcgaaaa atatacaact
     7981 tttaaaggca gcatttcatt ttttaaagtc taacaaccat agaactgaaa tcagagcaag
     8041 gcatcaattt cctactctta gtacgcctat tttgatggtt gaagtgaaga tttttttatt
     8101 tagcgtagac aatttccaac ttcccaaaag ccacatctcc atggataatc aaggttttgc
     8161 tggttggggc tacaggagcg tcagccttag ctgcaccaaa ggaggtttct actttcaaat
     8221 ctacacgcca gtgttgtgga acatagacgg ttgcattccc gaaggccact tcaatattta
     8281 aagttgcaaa atcacctagc atctctgcat tatcatagta gattttagca tccccaaaag
     8341 cgacttccac ttggtcatct acgagatctt gatcttgctt atagaaggtc ccgctaccaa
     8401 aagcgacttc cttatccgtg acgactgttt ttttcccatt gtaccaccat tttttttcat
     8461 tccagaactt acttgaatgc gtcagataac caacaccaag taccaccaag atgctagccc
     8521 aaataagaga atgattggta actggtaaca agtcataagc gtaatttgca atgatgagcg
     8581 ccagtaaacc tgtaaaaact gccgaagtga gatgacgtct aaggatggac tcaatggact
     8641 tataagcaaa aaaaacaata cctagtaaag gccatatttt accatccaaa gaaggaattc
     8701 caaaattccc ttgcagcaag atccaagctg ctaaaaccaa taaaacaata ccaaatgctt
     8761 tctttttcat gttttttacc tcatgtttct tagattttct tttaggaggg attggtaatg
     8821 ccgtgagaca tgaacctcct tgtgcgtctg ataaaaggaa atggtgcccg ttcctgaaaa
     8881 ggacttgtcc actgagtaaa tctgacggat gtttgcgatc gttgacttgg aaactcgatt
     8941 aaaatagcga ggcaagatag actccaactc atagagcttg aggcgaacct cataagcttc
     9001 cttctggtta tgagcgtaga tcttgctccc ttctgtttca aagaagagaa tttctgacag
     9061 gtctagataa tattcacctg tccccttgta aaaagtcaac ctcggagcct ttgactcttg
     9121 caagagccgt tgcaagtctg caatttcatc tgtcaaagtg ggggtcttga tgacaatttc
     9181 agtctcctct aaattgccgt caatctcaat tcgtaacttc atcacatctc ctttctgact
     9241 ctactatatc aaagctaagt taggaataca agggcaaaaa agcaagtggt caatttgaag
     9301 cagtaagtgg ttgtaggact agcttaactt acatgtcaga ataggaagaa aaggaatccc
     9361 cacaccaaac cacaagcata gccaaccaag acatccttgg gataatgaac accccctaaa
     9421 accctcacca accccagcaa agctgaaaag atcaagcata ctaagccaat aggtagacta
     9481 gcatgcagac aggccatgga gatgatagtt gccgaaaaga catggcgact gggcatcgac
     9541 cgtcccgaac tatccttatc aagaagggga cagatatccc aagtttcata aggtctcggg
     9601 tgattgattt tcttacgaaa cagggacaag gccacaaaac ctgatgcagg aataagcaca
     9661 taaaccccaa cttgctgacc aagtcctagt tgcaggtaag tagtgactag caagatcaga
     9721 tagaccaggg gcatggcgac tgtcatcaaa cgattgaaac tgcgtaatag gaataaaaga
     9781 attggacgac tagttagtct ggatctgata tttcgatacc attcttgata atttttcatt
     9841 tatgcttctc atacttttct agaacttgtt gagatgtttt ccccttggca agccaatcca
     9901 ctagcttgtc caagcgacgt atttcctgca tcagaggatc atcaatttcc tcaatacgga
     9961 caccgcaaat cttccctgta attgcaattc gttcagggac ataatatggt gcttgtcgaa
    10021 aaaaatctcc gtaagtaaca tccctatcca gacaagcaag aacgtcactc acttcatacc
    10081 ctgtcaacca actggtcact tggtgaactg actctgcctt tccacccttt cgctcaactt
    10141 ttgcaatcaa ggctaagtag acagcagcaa atttcatatt gtataatttc tgactcatac
    10201 ttctctcttt ctatgtgtac tagtataaat aagaaaaaga aggccgtcaa gccttctttt
    10261 gatttattct tctgcttcat cttctgtaaa ttgactattg tacaagtcag cgtagaagcc
    10321 accttgcgcc atcagttcct catagttgcc ttgctcgatg atatttccat ctttcatgac
    10381 caagatcaag tctgcatttc ggatggttga caagcggtgg gcaatgacaa aggatgtgcg
    10441 tccttccatc aaacggtcca tggctttttg gatcaattcc tctgtccgtg tgtcaacaga
    10501 agaagtcgcc tcatccaaaa tcaaaagcgg tgcatcctta agaagggcac gagcaatagt
    10561 caatagttgt ttttgtccta cagacaaggt cacggtgtca tccaagatgg tatcatagcc
    10621 atctggcaag gtcataataa agtggtgaat tcccacagcc ttactagctt ccatcattcg
    10681 ttcatcacta atccctattt gattatagat gagattgtct cgaatagttc cttcaaagag
    10741 ccaggtatcc tgcaagacca ttgaaaaggc atcatgcact tctgaacgcg tcatagcctt
    10801 ggtatccaca ccatcaatgt gaatacttcc cttatcaatc tcatagaatt tcatcaaaag
    10861 attgacaatg gttgtcttac cagtcccagt cggcccaaca atggcaacct tttgaccagc
    10921 atgagctgtc gcagagaagt catagtcttg aacattgaca ccgtccacca gaatttctcc
    10981 tgctgacacg tcgtagaaac gtggaatcag attgaccaga gttgatttac cagaacctgt
    11041 tgacccaata aaggccactg tttgaccagt ttctgcttta aagctaacat gttcaataac
    11101 tgcctccgaa tttgccgcat agcggaaggt cacatcctta aactcgacct gacctttgaa
    11161 gttttcatca gtcagctgca cttgaacagg gttttggata gaagaatgca aatctaaaac
    11221 ttgattaatc cgcttagcag agaccatagt tcggggaaga acgatgaaga gtgctcccat
    11281 gagaaggaag cccatgacaa cctacatggc ataagacatg aaaacaatca tgtcactaaa
    11341 gagaggcaga cgcgctatcg gagcagcgtc gttaatcaca taggccccaa tccagtaaat
    11401 cgctacactc aaaccacttg aaatccccat catgatagga ttcaaaatag ccataagacg
    11461 gttgacaaac aaattcaaac gggtcaattc atcatttact gctgcaaatt tttcattttg
    11521 ataatcctct gcattgtagg cacgaacgac acgaatacct gttaaactct cacgagtgat
    11581 actgttcagt ttatctgtca acccctgaat caaggactgt tttggaaagg ctagcgtcat
    11641 caaaacggtc gtcatcagga cgttgacaat cactaccaca agtacggccc agagtcagta
    11701 ttctgaatga cctaaaatct tcccaatagc ccagatagcc ataattggac cacgcgttac
    11761 cacttgcaag cccatagtaa tcaacatttg aacttgagta atgtcattgg tagtacgcgt
    11821 caagaggcta ggaattgaaa atttcttaat ctctgtctgc gagtaatcca aaactcggtt
    11881 aaaaatatca cttctcagcc tactagtata agaagccgcc actcgggatg caaaaaatcc
    11941 aactgcaact acggacaaga aggcaagaaa gagcattccc atcatcatgc ttgccggctg
    12001 ccacaactca tctaaattag tttcttgact acctagcaaa tccgtaattt tcgagatata
    12061 ggtcggcact tccaactcta gatagaccga aaagcaagta aagagaatgg ctagtaaaat
    12121 catcccccat tcttttctac taattctttt ggttaatttc tttattctct cctcctattc
    12181 ccttgatatt ttgcctgtag ttgaccgaga actttctcaa aaatcagtaa ttcatcttca
    12241 tcaatgtctt ccatcaactg cttgtctatg cgttcaaaaa aagccttaac ctgttgcatc
    12301 tgagaacgtg ctttgtccgt cagacgaaca aacttagccc gcttatcaac aggactcgcc
    12361 tccaattcca ccaaaccatt ttgcactata cgcttaacca gattactagc aacagacttg
    12421 gtaatattga gttcctgctc gatatcttta atcaagacca agtcttggtt tttctagcga
    12481 ttatccaaaa aacgcacaac ctgaccttgc ggcccaccca taaattcaat gccgcaacgt
    12541 ttggcttcct tttgcaccat caggtaaatt tgatgaccaa aacgcttaaa gactaacatc
    12601 ggtttatcca taatctcccc cttctaaata aaaatagttc tctggagaat aattaaattt
    12661 ctatgagaac tattttcttg attaaaaaaa tcccaagtga ttttctcact taggatcatg
    12721 ttctataggt taaattaaaa cccatctacg ttcgtataaa tcttttggac gtcttcgtcg
    12781 tcttcaagaa cgctgtaaag tttttcaaag gtttcaaggt cttcgcctga caattccact
    12841 tctgactgag gaatcatttc caattcagtc acttggaatt cttcaatacc agactcacgg
    12901 agagcaacga tagccttgtg aaggtcagtt ggcgctgtgt aaactgtgat tgtaccttct
    12961 tgtgcttcta cgtcatccac atccacatcc gcttcgagca attgctcaaa gactgcgtcc
    13021 gcatcttcac ctccaaatac aataacacct ttgttgtcaa aaaggtaaga aacagaacct
    13081 gaagcgccca tgtttccgcc gtttttacca aaggctgcac ggacattggc tgctgtacgg
    13141 ttaacgttag aagtcaaagt atccacaatt agcatagagc catttggccc aaaaccttcg
    13201 taacgtcctt ctgtaaaggt ttcgtctgtg tttcctttgg ctttatcaat cgctttatcg
    13261 ataatgtgtt ttggcacttg ggcttgttta gcacggtcga taacgaattt caaagctgag
    13321 tttgattctg gatctggatc acctttttta gctgctacat agatttctac accaaatttt
    13381 gcatatactt tagagttagc tccatcttta gccgttttct tggctacgat attggcccat
    13441 ttacgtccca ttaggaatct ccttttttca cattttaatc tttcttatta taacacaagt
    13501 ttttttgatt ttcactagag gaaatggatt ttattagcaa atcaagctag gatagcactt
    13561 tacctgctaa gatggtcttg cctttctatc tttatcaaca gacactcatc cacattcaaa
    13621 aaacaaacta gaccattatc tgcaaataga aagtttcagc caagtttgac aaagtcagct
    13681 caaattactg tttgaagttt gtagatataa gcgacaaaaa caatcatact gcaccttttg
    13741 ttgacagtct actccagaca tatcatagtt caagtaaata ctttgaaatt caacagttct
    13801 tataggcgct attgtattct aagaaatcaa tagaagagtt tctaagcaaa cctctaatac
    13861 tcaataaaaa tcaaagagca aactagaaag ctagcctcag gttgctcaaa acactgtttt
    13921 gaggttgcgg atggggctga catggtttga agagattttc gaagagtata atttacgtgt
    13981 tcccaagatg gagaagttag actagtacac tggcacttct aaaacattgc tagcaattga
    14041 tttgttcata tttaatttca ttttttccat aaatgggtat tagatataaa cagcaaaata
    14101 tttccgatac gtgtcgttct tgaatttcca atcatctaaa acaagtaaag gataatcaat
    14161 cccctgtata tcaaggaatt ggctaccctt tttacttttt tacacattct gtttgataga
    14221 ttcattttaa catcacgagc atactccaat ggaaatcgct aggcaagaga taaactttca
    14281 gatatccgca gagagatcat cgcctctttt tgtcgcaagc attctcctct cctagtcatt
    14341 ttctacctta tcttctacct gaggatagag agttgttccc caaatagaaa tcgtccgctt
    14401 acgcactagt ggcaaatcgg ttttttcata aaccgtacgc caccattccc aggcaagccc
    14461 ggtacactct ctaattttga cagagagatt acgaacattc ccttttaaag gaatactagt
    14521 ggtaaagtga gccgttaaat cctgcccatt tctgtcccaa gccttaggag tcaagacttc
    14581 cttaccttga tgatcatagg ataattcatt ccaagtaata taatattggg caacataggc
    14641 accactatga tccagcagta aatctccgtt tctgtaagct gtaaccttag tctcaacata
    14701 gtctgtacta ttttgaaagg tcgcaactac attgtcacgt aaaaaagaag ttgtatagga
    14761 aatcggcaag cctggatgat ctgctgtaaa gcgactgcct tcttgaatca agtcctctac
    14821 catatccacc ttgcctgtta caactcgggc acccgaactt gggtcgcccc ctaaaataac
    14881 cgccttcact tctgtattgt ccaaaatctg cttccactct gtctgaggag ctaccttgac
    14941 tccttttatc aaagcttcaa aagcagcctc tacttcatca ctcttactcg tggtttccaa
    15001 cttgagatag acttggcgcc cataagcaac actcgaaata tagaccaaag gacgctctgc
    15061 agaaattcct ctctgtttta aatcctctac cgttacagta tcttgaaaca catctcctgg
    15121 atttttaaca gcatctacgc tgactgtata ataaatctgc ttaaaattaa caatctgaat
    15181 ctgcttttcg cctgaatgga cagagttaaa atcaatatca agagaattcc ctgtcttttc
    15241 aaagtcagaa ccaaacttga ccttgagttg ttccatgctg tgagccgtga ttttttcata
    15301 ctgcattcta gctgggacat tattgacctg accataatct tgatgccact tagccaacaa
    15361 atcgtttacc gctccgcgaa cactcgaatt gctggggtct tccacttgga gaaagctatc
    15421 gctacttgcc aaaccaggca aatcaatact ataagtcatc ggagcacgat cgaccgcaag
    15481 aagagtggga ttattctcta acaaggtctc atccactacg agaagtgctc caggatagag
    15541 gcgactgtcg ttggtagctg ttacagaaat atcacttgta tttgtcgaca agctccgctt
    15601 ctttctttcg ataacaacaa actcatcggg tagctgatta ccctctttga tgaaacgatt
    15661 ttcaatactt tctccctgat gggtcaagag tttcttttta tcgtaattca tagctagtat
    15721 aaagtcattt actgctttat ttgccatctt ctacctccta ataagttcct ggattgagtt
    15781 gcataaactc agacttgttc agcgaaatca gccgtggttg gactaagtaa tccaaaattt
    15841 cctcgtacaa ttcttctgag acattgcgtc gccgtctggc taaataagaa gtcggaatga
    15901 ccgtattatc caacataaat accttatcta agtcaatcaa ggttggtctt gtaaaaggat
    15961 tacgagctag atccggctct tctatcataa agttcttgac caaacgtctg gtcaagagag
    16021 ctggtttgaa ggtctgattt ttaaccaact ctttgttttt agtcatgctg ttgtcaatac
    16081 agatatacat atgattcttc acagccaaat cgctactaat agtcggaaaa ggcaaataaa
    16141 gagctacaac atctcctctc ttaatcaagc aagagcaccc ccttttctcc taatgtaaca
    16201 tagacaggat tgaccaagtc ttctgattga ctcagaattt ccaaagtttg agtttggcgc
    16261 gctgtcaatt tagtagcatc ttgtctcttc aatacaaaat gcttgtcgcc aataaccttg
    16321 acaatataat ccttctccaa agctgactgg taaatccaca tcagatgttg tctgtcctga
    16381 gaactcaaga gagaaggatt ttcaagcctc ccgatagtct gataaaaatc aaaaacagga
    16441 gctaactcct gccaatctga ttggctagtt gtcaaggcta gaaaaagggc tttgcgagct
    16501 gatacttctt ggttagcctt gagagttact ttcccctcca agttttttag aaatcgggaa
    16561 actccagaaa gcaaattttt ctctaactgc gagaaataaa aacctttcgt tcccagacat
    16621 aagtctttca tgtcgctttc tctagcaaat aagagctcaa acatttgata gtaaaagaaa
    16681 aatatctggc actgggtcgc gctcatcttt tccttatcgg cttctttttt taaccagagc
    16741 aagggcgaca ggtagctgga ttgagacatt tcctctacct cctactcttt tttaactgga
    16801 gcatctgcac tagctgccac ttcttttgac tggatacttt cccactggtt aatctcctct
    16861 gagataagac cttcgcatgt cttgacaaat agggcaaaag ccttggtctt tcctgcatat
    16921 ttctccgttt ggcattgata gaggaatttt tctttctcca ggagttgcgc agttttttgg
    16981 taagaaatcc aattttcctt tgcattatac aaattgataa tcccctcaca cagcaagccg
    17041 agactggata aggcaaccga aatcaaacgg tagcgatcac ctggcatagg aatagcacaa
    17101 aagacagcta tgaggaaacc tgccacgatt tctgttattt ttaatacctt atagcgccta
    17161 cgatgttgaa cgcttttctt taaaaaatga gctatctgta cgtctaatcg ctctgtcagg
    17221 tacatttctt ctggcgtcat attcgtaact cctttcattt actttgataa tcatagtata
    17281 acatatttga atatcaaagt atagtaaaaa ctggactata act
//
LOCUS       DAVKWS010000040        17242 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKWS010000040 DAVKWS010000000
VERSION     DAVKWS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.040"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            702..1016
                     /locus_tag="VW852_001479"
     CDS             702..1016
                     /locus_tag="VW852_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV0272603.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1032..1418
                     /locus_tag="VW852_001480"
     CDS             1032..1418
                     /locus_tag="VW852_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV0272604.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1447..2832
                     /locus_tag="VW852_001481"
     CDS             1447..2832
                     /locus_tag="VW852_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV0272605.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2835..2987
                     /locus_tag="VW852_001482"
     CDS             2835..2987
                     /locus_tag="VW852_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV0272606.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3010..4428
                     /locus_tag="VW852_001483"
     CDS             3010..4428
                     /locus_tag="VW852_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV0272607.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMEI
                     RLRSKLKSVLIVHYLKILYNRN"
     gene            4477..4560
                     /locus_tag="VW852_001484"
     CDS             4477..4560
                     /locus_tag="VW852_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEV0272608.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            4685..5422
                     /gene="erm(B)"
                     /locus_tag="VW852_001485"
     CDS             4685..5422
                     /gene="erm(B)"
                     /locus_tag="VW852_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV0272609.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(5679..6926)
                     /locus_tag="VW852_001486"
     CDS             complement(5679..6926)
                     /locus_tag="VW852_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0272610.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            7106..7327
                     /locus_tag="VW852_001487"
     CDS             7106..7327
                     /locus_tag="VW852_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272611.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            7444..7941
                     /locus_tag="VW852_001488"
     CDS             7444..7941
                     /locus_tag="VW852_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV0272612.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            7916..8422
                     /locus_tag="VW852_001489"
     CDS             7916..8422
                     /locus_tag="VW852_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV0272613.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            8406..10853
                     /locus_tag="VW852_001490"
     CDS             8406..10853
                     /locus_tag="VW852_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0272614.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            10856..13033
                     /locus_tag="VW852_001491"
     CDS             10856..13033
                     /locus_tag="VW852_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV0272615.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            13030..14031
                     /locus_tag="VW852_001492"
     CDS             13030..14031
                     /locus_tag="VW852_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV0272616.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            14028..14963
                     /locus_tag="VW852_001493"
     CDS             14028..14963
                     /locus_tag="VW852_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV0272617.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            15208..15324
                     /locus_tag="VW852_001494"
     CDS             15208..15324
                     /locus_tag="VW852_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV0272618.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            15340..>17242
                     /gene="tet(M)"
                     /locus_tag="VW852_001495"
     CDS             15340..>17242
                     /gene="tet(M)"
                     /locus_tag="VW852_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV0272619.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYM"
BASE COUNT         5632 a         3070 c         3570 g         4970 t
ORIGIN      
        1 aagttaaaag aataaataat tctttatagt aataaaaaaa aaaaaagttc aaaatgcaat
       61 aaaaataatt gactgaataa actacatatg ttagaataaa aacaaggaaa aagaaagggg
      121 tttcattgca tgagaaaaaa acgtggaatt aaaaaattag ttacatttgc atgactaggt
      181 gtttttatgt ttagtaatac aattccttac caacagttta ttcagaagaa taaacaattg
      241 gagattcgag tgcaatcaca aaaggagtcc aatggtcttg atgttgggaa ggctgattag
      301 ttgattaaat gatgaaaata ctaattaact atatggtatt tctgttttat ctaattttta
      361 attagaaaaa tagcataaaa atctagttat ccgcataaaa actggactta tcacacttta
      421 tcaaggtcaa aaccactcaa tttactacta atttactact tatgaatgag ctttgatacg
      481 acgatttatc cttgaaaagt gaagatataa agatacttcc aataaaattt gaatatttaa
      541 taggtagaca cttcaaaaaa tgaggtgtct atttttttac ccgattttga aaggaagtga
      601 acttatgaaa acaaaaaatc aagaatcaaa aggtcgttcc ccactcttta agaccatcaa
      661 acattcattc agccaataaa aaagaaagga taggtaaaaa tatggaactt aaatttgtga
      721 ttcccaacat ggaaaaaaca ttcggcaatt tagaatttgc tggcgaggat aaagtcgttc
      781 agcgaagaat caacggacgg ctaactgtct tatcaagaag ctataatctc tattctgatg
      841 ttcaaagagc agatgatatt gtggtggtgc ttcctgctga agctggcgaa aaacatttcg
      901 gctttgagga acgtgtgaag ttagtcaatc cacgtattac cgcagagggc tacaaaatcg
      961 gcactcgtgg ttttacaaat taccttttac atgctgacga catgataaaa gaataaagaa
     1021 agagaggaaa aatgatgaga ttagcaaatg gcattgtatt agataaagac acgacttttg
     1081 gagaattgaa attctctgct ctacgtcgtg aagtgagaat ccaaaatgaa gacgggtcgg
     1141 tttcagatga aatcaaggaa cgtacctatg acttaaaatc caaaggacaa ggacgcatga
     1201 ttcaagtaag tattcctgcc agcgtgcctt tgaaagagtt tgattataac gcacgggtgg
     1261 aacttatcaa tcccattgcg gacaccgttg ctactgccac ctatcaagga gcagatgttg
     1321 actggtatat caaggcagac gatattgtgc tgacaaagga ttctagttca ttcaaagctc
     1381 aaccacaagc aaagaaagaa ccgacacaag acaaatagtc gctaggtaga aaggagactt
     1441 tttcgcatga aacagcgtgg taaaaggatt cgcccatctg gtaaagattt agtctttcat
     1501 tttacgatag cgtcactcct gcctgttttc ctgctggttg tcggactgtt tcatgtgaag
     1561 acaatccagc agatcaactg gcaggatttt aacctatcac aagcagataa gattgacatt
     1621 ccctatttaa ttatcagttt cagtgtcgca attcttatct gcttgctggt agcgtttgta
     1681 ttcaaacggg ttcgctatga tacggttaaa caactttacc accgtcaaaa actggcaaag
     1741 atgatacttg aaaacaagtg gtatgaatct gaacaggtca aaacagaggg tttctttaaa
     1801 gatagtgctg gtcgtacaaa ggaaaagata acctacttcc ctaaaatgta ttatcgactt
     1861 aaaaatggct tgatacagat acgggtggaa atcacgctgg gaaaatatca agaccaactc
     1921 ttacacttgg aaaagaaatt agagagtggc ttgtactgtg agctgacgga taaagagtta
     1981 aaggattcct atgtggaata tactttgctc tatgacacca tagccagtcg tatttctatt
     2041 gatgaagtag aagctaaaga tggtaaactt cgcttaatga aaaacgtatg gtgggaatat
     2101 gataagctcc ctcatatgtt gattgctggt ggtacaggtg gcggtaaaac ttactttata
     2161 ctgacactga ttgaagcctt gcttcataca gattcaaaac tgtatattct tgacccgaaa
     2221 aatgctgacc ttgcggactt aggttctgtg atggcaaatg tctactatag aaaagaagac
     2281 ttgctttctt gcattgaaac attctatgaa gaaatgatga aacgtagtga ggaaatgaag
     2341 cagatgaaga actataagac tggcaaaaat tatgcttact taggtctccc ggcacacttc
     2401 ttaatctttg atgaatacgt cgctttcatg gaaatgctgg gaacaaaaga aaacaccgca
     2461 gttatgaata agctgaaaca gattgtcatg ttaggtcgtc aagctggctt ctttctaata
     2521 ctggcttgtc aacgtccaga cgcaaaatat ttaggcgacg gaatccgtga tcagtttaat
     2581 ttcagagtgg ctttaggtcg tatgtctgaa atgggctatg gcatgatgtt tggcagtgac
     2641 gtacaaaagg atttcttctt aaagcgaatc aaaggtcgtg gctatgttga tgtaggaaca
     2701 agtgtcatat cagagtttta tactcccctt gtaccaaaag gatatgattt cttggaggaa
     2761 attaaaaagt tatccaacag cagacagtcc acgcaggcga cgtgcgaagc ggaagtcgca
     2821 ggtgtggact gatcttgctg gctggtgtgg caatagccac gccagcactt aaccccccgt
     2881 atctaacagg ggggtacaaa tcgacaggaa acagtcaaaa aaacattaga aaatcctttg
     2941 gttacaaggg atttacaaaa tttcagcgta tgtcaaatgg gctttaaaag ttgacatacg
     3001 cctttttgat tggagggatt tttactgaat gaacaaactt ggttacagca tttaaaagaa
     3061 aaacgcttgg cttatggact atctcaaaac cgtttagctg ttgcgactgg tattacaagg
     3121 cagtatctaa gcgatattga aacaggaaaa gtcaagccat cagaggattt acagcagtcc
     3181 ctttgggaag ctctggaacg cttcaatccc gacgctcccc ttgaaatgct gtttgattat
     3241 gtaaggattc gctttccgac aacagacgta cagcaggtgg tcgaaaacat cttacaactg
     3301 aaactgtcct attttcttca tgaggactat ggtttctatt cttattcaga gcattatgct
     3361 ttaggcgaca tattcgtcct ttgctcccat gaactggaca aaggagttct ggtggaattg
     3421 aaaggtcgtg ggtgcagaca atttgaaagc tatcttctgg cacaacaaag aagctggtat
     3481 gagttcttta tggacgtttt ggtggctggc ggtgtgatga aacgccttga ccttgccatt
     3541 aacgataaga cagggatttt gaatatccct gtactcactg aaaagtgcca acaggaagaa
     3601 tgtatctccg tcttccgcag ttttaaaagc tatcgcagtg gcgaactggt acgcaaagag
     3661 gaaaaggaat gtatgggaaa caccctctat atcggttcat tacaaagtga agtttatttc
     3721 tgtatctatg aaaaggacta cgagcagtac aagaaaaatg atattcccat tgaagacgca
     3781 gaagtaaaaa accgttttga gattcgattg aaaaatgagc gtgcctatta tgcagtccgt
     3841 gatttactcg tctatgacaa tccagagcat accgccttta aaattatcaa tcggtatatc
     3901 cgttttgtag ataaagacga ttccaaacct cgttctgatt ggaaactgaa tgaagaatgg
     3961 gcttggttta ttgggaacaa tcgtgaacga ttaaaactaa ccacaaaacc agagccttac
     4021 tccttccaaa ggacgctgaa ctggctatct catcaagttg ccccgacctt aaaggttgcg
     4081 attaaacttg atgaaatcaa ccagacgcag gttgtaaaag acattctcga ccatgcgaaa
     4141 ctgacagacc gacacaagca gattttgaag caacagtcag taaaagaaca ggacgtgata
     4201 acaacaaaaa aaggatatct gtcaaccata ccagttgaca gatatccaaa aaaagatata
     4261 atgggagata agacggttcg tgttcgtgct gacttgcacc atatcataaa aatcgaaaca
     4321 gcaaagaatg gcggaaacgt aaaagaagtt atggaaataa gacttagaag caaacttaag
     4381 agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag ttaaattaga
     4441 tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat
     4501 gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc agataaataa
     4561 atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca gataactaaa
     4621 attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact tcaggagaga
     4681 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
     4741 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
     4801 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
     4861 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
     4921 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
     4981 gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccgtgc
     5041 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
     5101 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
     5161 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
     5221 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
     5281 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     5341 agtaaacaat ttaagtacca ttacttatga gcaagtattg tctattttta atagttatct
     5401 attatttaac gggaggaaat aattctatga gtcgcttttt taaatttgga aagttacacg
     5461 ttactaaagg gaatggagat aaattattag atatactact gacagcttcc aagaagctaa
     5521 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     5581 gctcgggacc ccttgtagga aaatgtccta agtgggatat ctgtcaactg gtatggttga
     5641 ctaaaaatac ttcctacgaa aatgtagggg gtattttttt acgaaaaaat acaatcgatt
     5701 cttaaaaaga aaaatttttg attggcaaaa ccataacaag ttcgttttag ggttttgatt
     5761 ttgcgattga tgccttctaa aggaccatta gagtattcaa atttagcgct atttaagaca
     5821 tattttctgt tttgacgaag ggtttgaata gcagtatcca tttctgtatt ggttttttgg
     5881 tagtctaaga tggttgactc tagtaattca ctattgcgct cgtttagggc tttcgtgata
     5941 tcttggtaag tttggtatac ttcagcgaac ttggaaaatt tactagtaat gagatcaaca
     6001 gcattttggc gagtcatata ttgtttaacg ccgcgaagaa aaactacttc ttcagggtga
     6061 agatcttcag cttttttatg gaatagcttc caatgtgact tcataatttt atattcttgg
     6121 ctctgtttat caagttgctt taggatagag atacgacaat tgtccaaagc gcgaccagct
     6181 aattgtacaa ggtggaagcg atcaataatg atattggcat tagggaaaag gcgatagata
     6241 aaactttgat attgagcatt taaatcaatt acaactgatt gaacgcattc gcgttcggct
     6301 tttgaataac gactttcaaa ataatcaaca atggtaggtg atagacgatc ctgtaacttt
     6361 gtgacaattt ggtgggtttc agcgtcacaa cagataaagg acatcacaga cttaattgaa
     6421 cgaaactcgt caaaacatag atgcttaggc aacttagcca cacgatagtg tggttccatg
     6481 cgctctaaga ttgttcgacg aacactgcta ggagagcagt gacacatttc agcaataagc
     6541 tgaccagata agcctttacg agctaaaagc atgatttgat ttttgagatc actggataag
     6601 gtttgatttt ctttggttaa attagtaata gcaccaaaag tagtatggca tgatttacat
     6661 ttatagcgtt gtttacgaag ctctagttca tatcttctcc catttaaact tgccagtcgt
     6721 acatgagttt tgcgaaagcc atccttatta actgtgggaa agccacagtt acgacaacga
     6781 ttaatcggat aagaaagagt agctgttatt agcgttatat actctttaac agaatcgttg
     6841 ttgtgttcag cttcttcaac agaaataatt ttaatatttt tatctttaat tccaagaata
     6901 tttaggatag aatcattatg ggacatttgt ttaaccttct ttcatgattt ttgtggtgaa
     6961 ttgattgtat aacgagggga cagcaaatgt cctctttttt gtataaaaaa atctggcatg
     7021 gaatctctat ccataccaga aagtgtatac cccaaaaaaa taactcaaat acaaattcat
     7081 tgaatataga gaggagaaca tttttatgaa ttttggacaa aacctttata actggtttct
     7141 atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt atcttggctt
     7201 caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg cggttggctt
     7261 agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc gcattattgg
     7321 tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt ccgcaaagga
     7381 atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa aggacgtgaa
     7441 tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga gggcgaattg
     7501 gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa aatcggcttg
     7561 aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac ggttgacgaa
     7621 tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt acccgaagaa
     7681 ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga actaagcgaa
     7741 catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt ggcacgctac
     7801 tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa ctatattgat
     7861 tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac caatcatggg
     7921 atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt ttctatttta
     7981 cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca tgaagaaaat
     8041 acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca atgattttag
     8101 acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac tctttgcagt
     8161 gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc tcaaatactt
     8221 tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg gtaaaaagcc
     8281 ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga cctatgcagg
     8341 aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag ttaggagtga
     8401 attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat aaagacgggg
     8461 aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt ccagaacaga
     8521 aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat ggcaaaattc
     8581 atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt tccaaaaatg
     8641 aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag acagacgctt
     8701 taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc tttaagttgc
     8761 ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa aatgctttgt
     8821 ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt atgagtaatg
     8881 atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc tctcgtcgtt
     8941 tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac ctttacggac
     9001 agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag ctggataatg
     9061 aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg gaagaaaaac
     9121 agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac tttaccatta
     9181 acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac cagcaacagc
     9241 aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat cgtaaagccc
     9301 tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac gcttggcaaa
     9361 gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat gagctggaaa
     9421 ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta agggtatcag
     9481 caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt tatgacgatt
     9541 taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa gaatttttac
     9601 ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat ttcctcgctg
     9661 gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat gttggctaca
     9721 gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa ggggttaagg
     9781 gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt ggtaaatcct
     9841 ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca gtgattgtag
     9901 acccaaaagc agaacgtggc agatggaaag aaaccttgcc agagatttcc catgaaatca
     9961 atatcgtcac tctgacttct gatgagaaaa acaaaggctt acttgaccct tatgtgatta
    10021 tgaaaaatcc caaagattct gaatcactgg ctattgatat tctgacattc cttacgggga
    10081 tttcctctcg tgatggggaa cgcttcccaa tccttagaaa agccattcgt gcagtaacca
    10141 atagtgaagt acgagggttg atgaaagtga ttgaggaatt acgggttgag aatacgccac
    10201 taagtaccag tatagccgac catatcgaaa gttttacaga ctatgacttt gcacatttat
    10261 tattcagtaa tggttatgtg gagcagtcta tcagcttaga aaaacaactg aacattatac
    10321 aggttgcgga cttggtactt cccgacaagg aaacttcctt tgaggaatat accactatgg
    10381 agcttttatc cgttgctatg ctgattgtca ttagtacctt tgctttagac tttatccata
    10441 cagaccgaag cattttcaag attgtagatt tagacgaagc atggagcttt ttacaggtag
    10501 cacaaggaaa aacactatct atgaagctgg ttcgggctgg tcgtgctatg aacgctgggg
    10561 tatatttcgt gacccaaaat acagacgacc tcttagatga aaaactgaaa aataacctcg
    10621 gcttaaaatt tgcatttcgt tccactgacc ttaacgagat taaaaagacc ttagcctttt
    10681 ttggtgtaga cccagaggac gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc
    10741 aatgccttat cagtgattta tatggtcgtg tcggtgtgat acagttccac cctgtatttg
    10801 aagaactgct ccatgccttt gataccagac cacctgtgcg aaaagaggtg taaatgtgaa
    10861 accatcaata gtaaacagaa taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc
    10921 aatgacagtg gctttcacac ttgtgattgc catttttctt ttagccatgc tgggaacggt
    10981 ggttcaagct gcgggcttgg tagatgatac ggtcaatgtg gcaaatgaat acagccgata
    11041 cccacttgaa aactatcaac tggattttta tgtggataat agctggggct ggcttccgtg
    11101 gaactggtcg gacgggattg gaaaacaggt catgtatgga ctatatgcca ttaccaattt
    11161 tatttggaca atcagtttgt atgtttccaa tgcgacaggt tacttagtac aggaagccta
    11221 ttccttagac ttcatttccg ctacagcaga ttccattggt aagaatatgc agaccttagc
    11281 tggtgtgagt gcaaacggat tttcaacaga gggtttctat gttggattcc tcttactctt
    11341 gattttggtt cttggggttt atgttgccta tacgggactg ataaagagag aaaccacaaa
    11401 ggcaattcat gccattatga attttgtgct ggtgtttatc ctatcggctt cctttattgc
    11461 ctacgctccc gactacatta aaaaaatcaa tgacttttca tcagacatca gtaatgccag
    11521 tttatcactt ggcacgaaga ttgtcatgcc ccattccgat agtcaaggca aggacagcgt
    11581 ggacttaatc agagatagcc tgttttccat acaggttcag caaccgtggc tactgcttca
    11641 atacaacagt tcagacattg aaagtatcgg tattgaccgt gtggaaagcc tgctctccac
    11701 cagcccagat tccaacaatg gcgaagacag agaaaaaatt gttgcggaag aaattgaaga
    11761 cagaagcaat accaatctaa ccattacaaa gaccattaac cgtttaggta cagtcttctt
    11821 cctatttgtc ttcaatattg ggatttccat atttgtattc ctattaacag gaatcatgat
    11881 tttctcgcag gtacttttta tcatctatgc tatgtttctg cctgtgagct ttattttaag
    11941 catgattcca tcatttgatg gtatgtcaaa acgagccata acaaagctct ttaataccat
    12001 tttgacacga gctggaatca cattgattat tacgacagca tttagtattt caaccatgct
    12061 ctatacctta tcggctggtt atccgttctt tttgattgct tttctacaga ttgtgacctt
    12121 tgcaggaatc tacttcaagc tgggcgattt aatgagtatg ttttctctac agagtaacga
    12181 ttctcaaagt gtgggaagtc gtgtgatgag aaaacctcgt atgcttatgc acgctcacat
    12241 gcaccgtcta cagcggaaac ttggacgttc catgactact ctaggggctg ggtctgccat
    12301 tgttacaggt aaaaaaggac agtcgggttc ggggagttct gcaaggacac aagcagatca
    12361 ctcccgacca gacggaaagg aaaaatcaac acttggaaaa cgtatcggtc aaaccatcgg
    12421 tacagtagct gataccaaag acagaatggt agacactgct agtggtttga aagaacaggt
    12481 taaagatttg ccgaccaatg caagatatgc agtatatcaa ggaaaatcca aagtaaaaga
    12541 gaatgtccgt gatttaacca gtagtatttc tcaaaccaaa gcggacagag ccagtggacg
    12601 caaggaacag caggaacaaa ggcgaaaaac cattgcgaag cgtcgctctg aaatggaaca
    12661 ggtcaaacag aaaaaacagc ctgcttcttc tgttcatgaa agaccgacta caagacaaga
    12721 acaatatcat gatgaacaga cctcaaaaca gtctaatatt cagacttcat ataaggaatc
    12781 tcaacaagcc aaacaagagc gtccagcagt taagtccgat ttttcaagtc caaaagtgga
    12841 acgccaaggc aataccgttc aagaaaaaac cgttcaaaag ccagcaactt caaccactac
    12901 agcagataga acttcacaac gtccaatcac aaaagaacgt ccgtctactg ttcaaagagt
    12961 accactacaa aatacaagaa gtagaccacc aatcaaaacc gccaccatta agaaagtcgg
    13021 taagaaacca tgaagttgaa aactttagtg attggtggtt ctggattatt cttgatggtc
    13081 ttctcactgc ttctgtttgt tgccatttta ttttcagatg aacaggacag cggaatttcc
    13141 aatattcatt atggaggtgt gaatgtttcc gcagaagtgc tggctcataa gcctatggta
    13201 gaaaaatatg ccaaagaata tggcgttgaa gaatatgtca acatacttct tgcgattata
    13261 caggtggaat cgggcggtac tgcggaagat gttatgcagt cctcggaatc cctcggtctt
    13321 ccacctaatt cattgagtac agaagaatcc attaagcaag gtgtgaagta tttcagtgaa
    13381 ttattagcca gtagcgaaag gctcagtgta gatttagaat cggttatcca gtcctacaat
    13441 tatggtggtg gtttcttagg gtatgtggct aatcgtggaa ataaatatac ctttgaactg
    13501 gctcaaagtt tctcaaaaga gtattcaggt ggcgaaaaag tgtcttaccc caatcccata
    13561 gccataccta tcaatggggg ctggcgatac aactatggca atatgtttta tgtgcaactg
    13621 gtaacgcagt atcttgtcac aacagagttt gatgatgata cggtacaagc catcatggac
    13681 gaagcactga aatatgaggg ctggcgatac gtttacggtg gagcttcccc gactacttct
    13741 tttgattgta gcggactgac acaatggacg tatggaaaag ctggaattaa cttaccacga
    13801 accgcacaac agcaatatga tgtgacccag catatcccac tatcggaagc acaagctggc
    13861 gatttggttt tctttcattc tacctataac gctggctctt atattactca tgttgggata
    13921 taccttggca ataaccgtat gtttcatgca ggcgacccaa tcggttatgc cgacttaaca
    13981 agcccctact ggcaacagca tttagtggga gcaggacgaa tcaaacaatg agaaaggaag
    14041 atttaatgat gaaatttaga aaaaatcaga ataaagaaaa acagatacca aaggaaaaga
    14101 aacctcgtgt ctactataag gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac
    14161 ttttagggct tagtttcagc tttgcgatat tcaagcactt tacagctata gatactcata
    14221 ctattcacga aacaactatc atagaaaagg aatacgttga tactcatcat gtagaaaatt
    14281 ttgtagagaa ctttgcgaaa gtctactatt catgggagca atccgataag tccattgata
    14341 atcgaatgga aagtctaaaa ggctatctga cagatgaact tcaagctctc aatgttgata
    14401 cagtacgcaa agatattcct gtatcgtctt ctgtaagagg atttcagata tggacggtag
    14461 agccaactgg cgacaatgag tttaatgtaa cctacagtgt agaccagctc attacagagg
    14521 gagaaaatac aaagaccgtc cactctgctt atatagtgag tgtctatgta gatggttctg
    14581 gaaatatggt actggttaag aatccgacca ttaccaacat acctaagaaa tcaagttata
    14641 aaccaaaagc cattgaaagt gaggggacgg ttgattccat tacaaccaat gaaatcaatg
    14701 agtttttaac gacgttcttc aagctctatc ctacagcgac agccagtgaa ctttcctact
    14761 atgtgaatga cgggatatta aaaccaatcg gaaaagagta catctttcaa gaactggtaa
    14821 atcctattca caatcgtaag gataatcaag tcacggtatc gctgacagtg gagtatatcg
    14881 accagcagac caaagcaacg caggtatctc aatttgattt ggtacttgaa aagaacggga
    14941 gtaattggaa gattatagaa taacaaatat tggtacatta ttacagctat tttgtaatca
    15001 cgtactctct ttgataaaaa attggagatt ccttgacaaa tatgctctta cgtgctatta
    15061 tttaagtatc tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
    15121 tcaatttgat agtgggaaca aataattgga tgtccttttt taggagggct tagttttttg
    15181 tacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg
    15241 ctttgtatgc ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
    15301 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
    15361 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
    15421 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
    15481 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
    15541 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
    15601 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
    15661 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
    15721 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
    15781 ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg
    15841 aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag
    15901 aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga
    15961 tttcagaatt gttctctgtt ccctgtttat cacggaagtg caaaaaacaa tatagggatt
    16021 gataacctta tagaagtgat tacgaataaa ttttattcat caacacatcg aggtcagtct
    16081 gaactttgcg gaaaagtttt caaaattgag tattcggaaa aaagacagcg tcttgcatat
    16141 atacgtcttt atagtggcgt actgcatttg cgagattcgg ttagaatatc ggaaaaggaa
    16201 aaaataaaaa ttacagaaat gtatacttca ataaatggtg aattatgtaa aattgataag
    16261 gcttattccg gggaaattgt tattttgcag aatgagtttt tgaagttaaa tagtgttctt
    16321 ggagatacaa agctattgcc acagagagag agaattgaaa atcccctccc tctgctgcaa
    16381 acgactgttg aaccgagcaa acctcaacaa agggaaatgt tacttgatgc acttttagaa
    16441 atctccgaca gtgacccgct tctgcgatat tatgtggatt ctgcgacaca tgaaatcata
    16501 ctttctttct tagggaaagt acaaatggaa gtgacttgtg ctctgctgca agaaaagtat
    16561 catgtggaga tagaaataaa agagcctaca gtcatttata tggaaagacc gttaaaaaaa
    16621 gcagagtata ccattcacat cgaagttcca ccgaatcctt tctgggcttc cattggtcta
    16681 tctgtagcac agcttccatt agggagcgga gtacagtatg agagctcggt ttctcttgga
    16741 tacttaaatc aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa
    16801 ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat
    16861 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
    16921 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
    16981 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
    17041 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
    17101 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
    17161 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
    17221 atagataaag tacgatatat gt
//
LOCUS       DAVKWS010000041        16411 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKWS010000041 DAVKWS010000000
VERSION     DAVKWS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.041"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            87..1865
                     /locus_tag="VW852_001496"
     CDS             87..1865
                     /locus_tag="VW852_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV0272620.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENSIFDMKRSTNEFIG
                     RSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKEPQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSG
                     VPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNEQKDQAVADDNEGDENDDADY"
     gene            1942..3168
                     /locus_tag="VW852_001497"
     CDS             1942..3168
                     /locus_tag="VW852_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV0272621.1"
                     /translation="MESLSQDERIFIFPNDLTHTPDLDKDQKIFETVNQKIKTGNVIG
                     FLGYGQERLTISSRFSDESNDHFLHYLLNKVLHINLTSLDVALSREERLYQLLVHLFP
                     KYLQAAIRKGLYKEYHRFSHNDSHVKGVIDVRNHLKKNLPFTGNIAYATREFTYDNPL
                     MQLVRHTIEYIKNQKSIGQGVLDNLSTSRENVSEIVRVTPSYKLADRAKIIRGNQSKP
                     IRHAYFHEYRNLQELCLMILNQEKHGLGYQDQKIYGILFDVAWLWEEYVYTLLPKGFV
                     HPRNKDKTDGISVFSVGKRKVYPDFYDRERKIVLDAKYKKLELTEKGINREDLFQLIS
                     YSYILKAEKAGLVFPSKDKVIDNEIGNLAGYGALLKKWSIQIPQKSSSYSAFCKMMEN
                     SEENFKAIIDEEVGRK"
     gene            3343..4329
                     /locus_tag="VW852_001498"
     CDS             3343..4329
                     /locus_tag="VW852_001498"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV0272622.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4393..4464
                     /locus_tag="VW852_001499"
     tRNA            4393..4464
                     /locus_tag="VW852_001499"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4426..4428,aa:Arg,seq:ccg)
     gene            4647..5345
                     /locus_tag="VW852_001500"
     CDS             4647..5345
                     /locus_tag="VW852_001500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV0272623.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5336..8875
                     /gene="smc"
                     /locus_tag="VW852_001501"
     CDS             5336..8875
                     /gene="smc"
                     /locus_tag="VW852_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV0272624.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8872..9666
                     /locus_tag="VW852_001502"
     CDS             8872..9666
                     /locus_tag="VW852_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0272625.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLAITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGNHKERSVIAYMEGL"
     gene            9666..10484
                     /locus_tag="VW852_001503"
     CDS             9666..10484
                     /locus_tag="VW852_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0272626.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10488..11765
                     /gene="ftsY"
                     /locus_tag="VW852_001504"
     CDS             10488..11765
                     /gene="ftsY"
                     /locus_tag="VW852_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV0272627.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11811..13298)
                     /locus_tag="VW852_001505"
     CDS             complement(11811..13298)
                     /locus_tag="VW852_001505"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV0272628.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(13447..14187)
                     /locus_tag="VW852_001506"
     CDS             complement(13447..14187)
                     /locus_tag="VW852_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0272629.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14187..16352)
                     /locus_tag="VW852_001507"
     CDS             complement(14187..16352)
                     /locus_tag="VW852_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0272630.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5127 a         2921 c         3632 g         4731 t
ORIGIN      
        1 taaataaggc aactgttggt gaaaagttta atgataaatt aaaagaagag tttctccagg
       61 aatggccttt ggataggatt ttaacaatgt caatcgatga atatgtaata ggaaagggac
      121 agcaaaataa gtctttatgc tacgctcttg agaagggaaa atacaaaaat ctatttcttg
      181 gaatttctgg tggctcagct tcaaaatttg gtatttattg gaataaaaaa acaaacaaat
      241 ataaagatca agctaataat gagatttcag agttggatca gcgattttca aaattaaaat
      301 cagatttgta tgaaattatc aaagaaggta ttcgttttaa ctttgaaaat tctatttttg
      361 atatgaaaag atcaacaaat gaatttattg gtcgttctgc tatggtgaca aaattacttt
      421 gtatctattc tgagggagat cctttctttg gtgtaaatat taatagtcag aaagaatttt
      481 ggaaccactt tgtttctcag acaaatcaag gtggacctta tctgcaaaat cataaaataa
      541 ttgaactggt gtccaaaact tatcctgagt tggagccatc gaaattagga actatgcttt
      601 ttgagtattc taagcttttt atggaaaata aggaagacaa tagtacaatg gattcatcaa
      661 acaattttcg tcatcaatta actcaatctc tattaaagtc tccaaacctt atccttcgtg
      721 gtgctcctgg cacaggaaag acttatcttg ctaaagaaat tgccaaagaa ttaacggatg
      781 gcaacgaaga ccaaatcgga tttgtacaat ttcacccatc ctatgattat acggattttg
      841 tagaaggttt aagaccagta tcaaatgggg atggagctat tgagtttagg ctacaggacg
      901 gtatttttaa agatttttgt cagaaagcaa aagaacccca attgattgga ggacaagata
      961 attttgatga ggcttgggat tcttacttag aatatataaa tgttgctgaa gaaaaagaat
     1021 atataacaaa aacatcttac ttatctgtta atagtagaca aaatttgtca gtaaattatg
     1081 atagtggtgt tccaggatgg tcactaccta gcaaatatgt ttacgagttg tataaagata
     1141 aaaattataa taagcaagaa tactacaaaa gtggtggaaa aactgtccta gaaacattga
     1201 gaaagagatt tggtttgaaa gactatgttt ccccaacaga aattgatact gataagaaat
     1261 tcgtcttcat catcgatgaa atcaatcgtg gggagatttc taagattttt ggcgaactct
     1321 ttttctctat cgaccctggc tatcgtggtg aaaaaggaag tgtttctacc caatatgcaa
     1381 atctacacga aactgatgaa aagttctata tccccgaaaa tgtttacatc atcggaacta
     1441 tgaatgatat tgatcgttca gtggatacct ttgattttgc tatgcgtcgt cgttttcgtt
     1501 ttgttgaagt tactgccgag ggtcaagttg gcatgttgga taaagagctg aatatccatg
     1561 cagaagaagc aaaaattcgt ctaagaaact tgaacgctgc tatcgaaaat gttcaggaat
     1621 taaacagtca ttatcatatt ggaccaagtt attttcttaa gttgaaggat gtagattttg
     1681 actatgaatt actctggtct gattatatta agcctctcct agaagactac ttgcgaggtt
     1741 cttatgatga ggttgaaact ttggaaactt tgaaaaaagc atttgaactg acaaataatg
     1801 agcaaaaaga tcaggcagta gctgatgaca atgaaggtga tgaaaacgat gatgcggatt
     1861 actgataatt aacacaagat tattaaagaa aaatttgttg aagaatatcc taaactaagc
     1921 aatctccttt tagacagaac cttggaaagt ctatcccaag atgaacgtat tttcattttt
     1981 ccaaatgatt tgactcatac tcctgatttg gataaggacc aaaagatttt tgaaacagtc
     2041 aatcagaaaa tcaagacagg gaacgtgatt ggttttcttg gatatggtca ggaaagatta
     2101 acaatttcct cacgattttc tgatgagagt aatgaccact ttttgcatta tctcttaaac
     2161 aaggttcttc atatcaatct cactagttta gatgttgctt tgtctcgtga agagaggctt
     2221 tatcaacttt tggtgcatct cttccccaag tatctacaag ctgctattcg aaaaggtctt
     2281 tataaggaat atcatcgatt ttctcataac gacagtcatg ttaagggagt gattgatgta
     2341 agaaaccatc tcaagaaaaa tcttcctttc acgggaaata ttgcctacgc aacgagagag
     2401 ttcacctatg ataatcccct catgcagttg gtccgtcaca ctattgaata cattaagaat
     2461 cagaaaagca ttggtcaagg ggtactagat aatctctcaa ctagtcgtga aaacgtatct
     2521 gaaatcgtgc gtgtaacgcc ctcttataaa ctagctgatc gtgctaagat tattcgggga
     2581 aatcaatcta aacctatacg tcatgcatac tttcacgagt acagaaactt acaagaactt
     2641 tgtctgatga tcctaaacca agaaaagcac ggtttagggt atcaagatca aaaaatctat
     2701 ggtattctct ttgatgttgc ctggctttgg gaagagtatg tttacacctt gttgccaaaa
     2761 ggttttgtac atcccagaaa taaggataag acggatggaa tttcagtatt ttctgttggg
     2821 aaacgaaaag tatatccaga tttttatgac agagaacgaa agattgttct agatgcaaaa
     2881 tataaaaaac tggaattgac tgaaaaagga atcaaccgtg aggacttatt ccagctgatt
     2941 tcctattctt atattttaaa agctgagaag gctggacttg tttttccgag caaggacaag
     3001 gttattgata atgagatagg aaacctagca ggttatggag ctttgttgaa gaaatggtct
     3061 atccaaattc ctcagaagtc ttcatcttat agtgcatttt gtaaaatgat ggaaaattca
     3121 gaagaaaatt ttaaagcgat tattgatgaa gaagtgggga gaaaataatt tcctctcttt
     3181 cttttatttt taacacaagt aaaacccgaa caatatattt caattcataa aataatttga
     3241 caaaaactgt actttggttt ataataaatt aaggaaacgt cggaaaagac gtagtgatgc
     3301 gagagcatag gtaggtcatt acaaaagaaa cgagacatcg atatgttaaa tgaatttcca
     3361 atttttgatt acgaagatat tcaattgatt ccaaataaat gtgtcattaa aagccgtgca
     3421 gaagcggata caagtgtcac tttaggaaat cacaccttta aactacctgt tgtgccagcg
     3481 aatatgcaga cgattttgga tgaaaatgta gcagagcaac tggctaaagg tggttacttc
     3541 tacattatgc accgttttga tgaggcagga cgcattcctt ttattaaacg catgcacgat
     3601 caagggctca ttgcttctat ctctgtcggt gttaaggatt atgagtatga ttttgttagc
     3661 cagctcaagg ctgatgctcc tgagtatatc actattgata ttgctcatgg tcatgcggat
     3721 agcgtgattt ctatgattca acacatcaag aaagaattgc cagatacctt tgtcattgct
     3781 ggaaatgtgg gaacaccaga agctgtgcgt gaattggaaa atgctggtgc ggatgctact
     3841 aaggtcggaa ttggtcctgg taaggtttgt atcaccaagg ttaagactgg ttttggtaca
     3901 ggtggttggc agttggctgc tctacgctgg tgtgccaagg ctgcgcgtaa accgattatc
     3961 gctgatggag gaattcgtac tcacggtgat attgccaagt ctatccgctt cggtgctagc
     4021 atgatcatga ttggttccct ctttgcagga cacattgaaa gtccagggaa aacgattgaa
     4081 gtcgatggtg aacagttcaa agaatattat ggttcagcct cacaatatca gaaaggtgct
     4141 tacaaaaatg tggaaggcaa acgtatcttg cttcctgcta aaggtcatct gcaagacact
     4201 ttaactgaga tggaacaaga ccttcaaagt gctatttctt atgcaggtgg acgtcaggtt
     4261 gctgacctta aacacgttga ttatgtgatc gtgaaaaact ccatctggaa tggggatgct
     4321 tcccactaag acggggtctt tgaccaaaaa acttgttatt agagcaaatt tctgttataa
     4381 taaaacaagt ttccaccctt agtgtaatgg atatcacgta agattccggt tcttgagatg
     4441 ggggttcgat tccctcaggg tggatgtaaa catcctaaaa agcctttaat agggcttttt
     4501 tctttatcct gacccaaatt tgccgctaaa actgagaatc tctgtctaaa gaattcccaa
     4561 atgctttcca gtcccgtttt agagtgactc agggggcttt ttttgatata ataaaaagga
     4621 ctattatcag ttagaaagaa gttggtatga aagaattaca aactgtacta aagaatcatt
     4681 ttgaaatcga atttgcagac aaaaagttac tggaaactgc ctttactcat acgagttatg
     4741 ccaatgagca ccgcctctta aaaatttcac acaatgaacg cttggaattt ttaggagacg
     4801 ctgttctaca gttattgatt tcagaatatc tatataaaaa atatcctaaa aagcctgaag
     4861 gtgacctatc aaaactccgt gctatgattg tccgtgagga gagtttagct ggttttgcgc
     4921 gtgattgcca gtttgaccag tttatcaagt tgggtaaagg ggaagaaaaa tctggtggtc
     4981 gcaatcgtga caccattctt ggtgatgcct ttgaagcctt tcttggtgcc cttcttttgg
     5041 ataaggatgt ggcaaaggtc aaggaattta tctaccaagt tatgattcct aaggtagaag
     5101 caggcgaatt tgagatgatt acagactata aaacccatct ccaagagttg cttcaggtca
     5161 atggtgatgt ggctattcgt tatcaagtga tttctgaaac agggcctgct cacgataagg
     5221 tctttgatgt agaagttctt gttgaaggta agagcatcgg tcaaggccaa ggtcgttcta
     5281 agaaattagc agagcaggaa gctgccaaaa atgccgttga gaaagggctg gattcatgta
     5341 tttaaaggaa atcgaaattc aggggttcaa gtcttttgct gataagacca aggtcgtttt
     5401 tgaccaaggt gtgacggcag ttgttggacc caatggatct ggaaagtcca atattacaga
     5461 aagtctgcgt tgggctttgg gggagtctag tgtcaagagt ctccgtgggg gcaagatgcc
     5521 ggatgtcatc tttgctggaa cagaaagtcg caaaccgctc aattatgctt ctgtagttgt
     5581 gactctggat aatcatgacg gatttatcaa ggatgcaggt caagaaatca gggtggaacg
     5641 ccatatctat cgtagtggag atagcgaata caagattgac ggcaagaaag tccgtctgcg
     5701 tgatattcat gacctcttct tggatactgg attgggacga gattccttct ctattatttc
     5761 ccaagggaag gttgaggaga tttttaattc caagcctgag gaacgacgag ctatttttga
     5821 agaagctgct ggagttttaa aatacaagac tcgcagaaaa gaaaccgaga gtaaactgca
     5881 gcaaactcag gataatctgg accgcttaga ggacattatc tacgagttgg ataatcaaat
     5941 caagcctctt gagaagcaag ctgagaatgc ccgtaagttt ttagacttgg aaggacaacg
     6001 taaggctatt tatttagacg ttctggttgc tcaaatcaag gaaaataagg cagaactaga
     6061 gtcgacagaa gaagagttgg ctcaggttca agaactcttg atgagttatt accaaaagcg
     6121 tgaaaaatta gaagaagaaa atcaaactct taaaaagcaa cgccaagatt tacaggctga
     6181 aatggccaaa gaccaaggca gtttgatgga cttgactagt ctgattagtg atttagaaag
     6241 aaaattagcc ctatcgaaac tggagtccga gcaagtggcc ctgaatcaac aggaggcaca
     6301 agctcgtttg gctgctttgg aggataagag aaattcactc agcaaagaaa agtatgataa
     6361 agaaagctct ttagctctgt tagagggaaa tctagtccaa aataatcaaa aactcaatcg
     6421 tttagaagct gaattgctgg ctttctcaga cgatcctgat cagatgattg agctcttacg
     6481 tgaacgcttt gtagctcttt tacaagaaga agcggatgtc tcaaaccagt tgacccgtat
     6541 tgagaatgag ttggaaaata gtcgtcagct ttctcaaaaa caagcagatc aactagaaaa
     6601 gctgaaagag caattagcta cagctaaaga gaaggctagt cagcaaaaag acgagcttga
     6661 aactgccaag gtgcaggttc agaaattatt ggctgactat caagctattg ccaaggagca
     6721 agaggagcag aaaacttcct atcaagctca acaaagtcaa ctctttgacc gtctggatag
     6781 tctcaaaaac aagcaggcca gagctcaaag tttggaaaat atcctgagaa atcatagtaa
     6841 cttttatgca ggtgttaaga gtgttctcca agaaaaagat cgcctaggtg ggattattgg
     6901 tgcagtcagt gagcatctga cctttgatgt ttattatcaa actgccctag agattgcctt
     6961 aggggcaagt agccagcata tcatcgtaga agatgaagag tcggcaacca aagctattga
     7021 tttcctcaaa cgaaacagag tcggtcgtgc aacctttctt cctttgacca ctattaaggc
     7081 gcgtacgatt tctagtcaga accaagatgc tatcgctgta agcccaggtt tccttgggat
     7141 ggcagatgag ttggtgactt ttgatactag actggaagcc attttcaaga acttgctagc
     7201 tacgacggct atctttgata ccgtagaaca tgcgcgtgaa gctgctcgac aagttcgtta
     7261 tcaggttcgt atggtgacat tggatgggac agaattacgc acgggtggtt cctatgcggg
     7321 tggtgccaat cgccaaaata acagtatttt catcaagcca gaactggagc aattacaaaa
     7381 agaaattgct gcagatgaag caagcttggg ttcagaagaa gcggctttga agaccttgca
     7441 agaccagatg gctgcattga cagaaagatt agaagccatc aaatctcaag gagagcaggc
     7501 acgtattcag gagcaaggct tgtccctcgc ttatcagcaa actagtcagc aagttgaaga
     7561 actggaaact ctttggaaac tccaagaaga ggaaatagat cgtctttctg agggagattg
     7621 gcaagcggat aaggaaaaat gtcaagagag ccttgctact atcgccagtg aaaagcaaaa
     7681 tctggaagct gagattgaag aaattaagtc taataaaaac gccatccaag aacgctatca
     7741 aaatttgcag gaagaggtgg cgcaagctcg cttgcttaag acagaactgc aagggcaaaa
     7801 acgttatgaa gtagctgata ttgagcgttt aggcaaggaa ttggacaatc ttaatatcga
     7861 acaagaagaa attcagcgca tgctccaaga aaaagttgac aatcttgaga aggttgatac
     7921 agaattgctc agtcaacagg cggaagaatc caaaactcag aaaacaaatc tccaacaagg
     7981 tttgattcgc aagcagtttg agttggatga tatagaaggt caactggatg atattgccag
     8041 tcacttggat caagctcgcc agcagaatga ggagtggatt cgcaagcaaa cacgtgctga
     8101 agccaagaaa gaaaaggtca gcgagcgctt gcgccatcta caaaatcaat taacagacca
     8161 gtaccagatt agctatactg aagcactaga aaaggcacat gaattggaaa acctcaatct
     8221 ggcagagcaa gaggtgcagg atttagagaa ggctattcgc tcattgggac ctgtcaactt
     8281 ggaagctatt gaccagtacg aagaagttca caaccgtctg gactttctaa atagtcagcg
     8341 agatgatatt ttgtcagcga aaaatctgct ccttgaaacc attacagaga tgaatgatga
     8401 ggtcaaggaa cgctttaaat caacctttga agctattcgt gagtccttta aagtgacctt
     8461 caagcagatg tttggcggag gtcaggcaga cttgatattg actgagggcg accttttaac
     8521 agctggtgtg gagatttctg ttcaacctcc aggtaagaaa atccagtcgc ttaacctcat
     8581 gagtggtggt gaaaaagctc tatcggctct tgccttgctt ttctccatta ttcgtgtcaa
     8641 gaccattcct tttgtcatct tggatgaggt ggaagctgcg ctggatgaag ccaatgttaa
     8701 acgttttggg gattacctca accgctttga caaggacagc cagtttatcg ttgtaaccca
     8761 ccgtaaggga accatggcag cggctgattc catctatgga gtgaccatgc aagaatcggg
     8821 tgtttcaaag attgtttcag ttaagttaaa agatttagaa agtattgaag gatgacaatt
     8881 aaactagtag caacggatat ggacggaacc ttcctagatg ggaatggacg ctttgatatg
     8941 gatcgtctca agtctctctt ggcttcctac aaggaaaaag ggatttactt tgcggtggct
     9001 tcgggtcggg gatttctgtc tctggaaaaa ttatttgctg atgttcgtga tgacattatt
     9061 ttcatcgcgg aaaatggcag tttggtagag tatcaaggtc aggacttgta tgaagcgact
     9121 atgtctcgtg acttttatct ggcaactttt gaaaagctga aaacgtcacc ttatgtagat
     9181 atcaataaac tgctcttgac gggtaagaag ggttcatatg ttctagatac ggttgatgag
     9241 acctatttga aagtgagtca gcattataat gaaaatatcc aaaaagtagc gagtttggaa
     9301 gatatcacag atgacatttt caaatttaca accaacttca cagaagaaac gctagaagct
     9361 ggtgaagctt gggttaatga acatgtccct ggtgttaagg ccatgacaac tggctttgaa
     9421 tctattgata ttgttctgga ctatgtcgat aagggtgtag ctattgttga attagctaaa
     9481 aaacttgcta tcacaatgga tcaggtcatg gcttttggag acaatcttaa tgacttacat
     9541 atgatgcagg ttgtgggaca tcctgtagct cctgaaaatg cacgacctga aattttagaa
     9601 ttagcaaaga ctgtgattgg taaccataag gaacggtcgg ttatagctta tatggagggc
     9661 ttataatggc agatataaaa ttgattgcat tggacttgga cgggaccttg ctgactactg
     9721 ataaaaggct gacggatcgt accaaggaaa ccttgaaagc tgcgcgtgat cgtggtatca
     9781 aggtcgtatt gacaactggt cgtcccttaa aagccatgga tttctttctc catgagttag
     9841 ggactgacgg tcaggaagat gagtatacca ttacttttaa tggtggatta gttcagaaaa
     9901 atacaggaga aatccttgat aaaacagtct tttcatatga tgatgtggca cgtttgtatg
     9961 aagaaacaga gaaattatca ctgcctcttg atgccatctc agaaggaaca gtttatcaaa
    10021 tccaatcgga ccaagaaagt ctttatgcca aattcaatcc agctttgacc tttgttccag
    10081 tggactttga agacttatct agtcaaatga cctacaacaa atgcgtgact gcctttgctc
    10141 aagaaccctt ggatgcagcc attcagaaga tttctccaga attgtttgac caatatgaaa
    10201 tctttaaatc acgtgaaatg ttgctagaat ggtcaccaaa gaatgttcat aaagcaacag
    10261 gtttggcaaa actaatcagc catcttggaa tcgaccaaag tcaagtgatg gcttgtggtg
    10321 acgaggccaa tgacctctct atgattgaat gggcaggtct tggtgttgct atgcaaaacg
    10381 ctgttcctga agtaaaggca gccgcaaatg tagtgacgcc gatgaccaac gatgaggaag
    10441 ctgtcgcctg ggctatcgaa gaatatgtgc taaaggagaa ctaagatatg ggattgtttg
    10501 accgtctatt cggaaaaaaa gaagaaccta aaatcgaaga agttgtaaaa gaagctctgg
    10561 aaaatcttga tttgtctgaa gatgttgatc ctaccttcac agaagttgag gaagtttctc
    10621 aggaagaagc agaggttgaa attgttgaac aagctgtgtt ccaagaagag gaaatccaag
    10681 acacagttga agaaagtctg gatttagagc cagttgtaga agtttctcaa aaagaagtcg
    10741 aagaatttcc acactcagaa gaagggaata ctgagtttct agagactata gaagaaaata
    10801 attctgaagt tcttgaacca gaaaggcctc aagcagaaga aaccgttcag gaaaaatatg
    10861 accgcagtct taagaaaact cgtacaggtt tcggtgcccg cttgaatgcc ttctttgcta
    10921 acttccgctc tgttgacgaa gaatttttcg aggaactgga agaactgctg attatgagtg
    10981 atgttggtgt ccaagtcgct tctaacttaa cggaggaact acgttacgaa gccaagcttg
    11041 aaaatgccaa gaaacctgat gcacttcgtc gtgtcatcat tgagaaattg gttgagcttt
    11101 atgaaaagga tggtagctac gatgaaagca tccacttcca agataacttg acagttatgc
    11161 tctttgttgg tgtgaatggt gttgggaaaa caacttctat cggaaaacta gcccaccgct
    11221 acaaacaagc tggtaagaag gtcatgctgg ttgcagcaga taccttccgt gcgggtgcag
    11281 tagctcagct agctgaatgg ggccgacgag tagatgttcc agtagtaact ggacctgaaa
    11341 aagctgatcc agccagcgtg gtctttgatg gtatggaacg tgccgtggct gaaggtatcg
    11401 atattctcat gattgatact gctggtcgtc tgcaaaataa ggataacctt atggctgagt
    11461 tggaaaagat tggtcgtatt atcaaacgtg ttgtgccaga agcaccacat gaaaccttct
    11521 tggcacttga tgcatcaaca ggtcaaaatg ccctagtaca ggccaaagaa ttttcgaaaa
    11581 tcacaccttt aacgggaatt gttttgacta agattgatgg aactgctcga ggaggtgtgg
    11641 ttctagccat tcgtgaagaa ctcaatattc ctgtaaaatt gattggtttt ggtgaaaaaa
    11701 tcgatgatat tggagagttt aactcagaaa actttatgaa aggtctcttg gaaggtttaa
    11761 tctaatcaga agcaaaaatc ctgcaaggcg taaacttgca ggaaattttt ttattctaag
    11821 cgaccatctt gacgataggt gatatctggt tgccaagtcc atttggcacc gaatttttca
    11881 agtaggtcaa agctggcttg aggtcccatg cttccagctt tatagtcatg aagtggggca
    11941 ccattttcag cccagagctt ttcaatacgg tcaatcaact tccatgacgc acaaacttca
    12001 tcccagtggc taaagttagt tgagttgtta tttaggacat cataaatcaa tttttcgtat
    12061 ggttctggag aagcaccagt tgcagtcgca tctgtacggt aatcaagtga gttaggagcc
    12121 aagttaaatt cttctcctac ttgcttccca tttaggctaa gagagaagcc ttctgttggt
    12181 tgaatataga tggtcaaaat atttggagca agtggttctc caaagataga atccatttgt
    12241 ttaaagacga tgttgacatg agttcctttt tcagtcagtc gtttacctgt acggaaaaag
    12301 aaaggaacac cacggaatcg atcgctgtct acaaagaagg caccagatgt aaaggtttca
    12361 gttgttgatt ctggattcac atttggctcg ctacgataag agatgtattt catgccatca
    12421 atcttaccag agcggtattg cccacggata aagtgttctt tgagttcttc atcagttgga
    12481 tgatagaggt ttttaaagac cttaatcttt tcagcacgaa tctcgtcttt tgtgaagctt
    12541 gctggtttgt ccatggcgag gagcgaaaga agttgtagag tgtggttttg gaccatgtca
    12601 cggagggcac cggattggtc atagtagcca ccacgttctt ctacacccaa gcgctccgca
    12661 aaggtaattt gaacattgtc gataaaatcc ttgttccaaa cgttttcaaa aatcaagttt
    12721 gcaaagcgaa ctgcaaagat gctttggatc atttccttac caagataatg gtcgatacgg
    12781 aaaatttgtt cttcgtcaaa tgttgctagg agttcgtcat tcaacttgtt tgcagttgcg
    12841 taatctgtac caaatggttt ttcaacgatc aagcgctcaa aacctttgcc atcgacaatg
    12901 ttttcagatt tgaggtgttt ggcaatggtt ccaaagaact gaggtgccat agacaagaag
    12961 aagagcttat tgtgttcagc ttggtatttt tcattaagct cagcttgtaa ttgacgcaaa
    13021 gcaatataat gttccgaatc attgacatca tggctttgat agtagaagtg gctagcaaat
    13081 tcttgggctt gctcggtact atctgccaaa tcaaggatgg actcgacaac tacagattca
    13141 aaatattcct tactccaagg tctacgggca gttccaataa cggcaaagtg cttggaaaga
    13201 ttgccggatt tatatagtct aaaaagggaa gggtagagct tgcgtttagc caggtctcca
    13261 ctcgcaccga aaattgtaac aataacctta gatgacatct agctacctaa tttctatttt
    13321 tattcctagt cttgctaggt atgaggaaac ctcatttcat aaacttaatt ctaacataat
    13381 tttccgaaaa atcaaaaatt ccaatacgag gtagaaaaac tgcaaggaaa tccttacagt
    13441 ttgagtttag acgtttaaga ccttatctaa gaactctttc agacgagggt gttgtgggtt
    13501 atcaaagatt tggtcaggtg ttccgtcttc aaggaactcg ccatctgcag taaagataac
    13561 gcggttggca acctgacgag caaatcccat ctcatgggtt acgataatca tggtcatgcc
    13621 ttgctcagcc aattccttca taacgttaag tacgtctcca accatctcag ggtcaagggc
    13681 agaagttggt tcatcgaaga gcatgatgtc tggattcatt gctaggccac gagctatggc
    13741 tacacgttgt ttttgaccac ctgataggct atctggattg gcattagctt tatctgctag
    13801 tccaaccttt tcaagcaact ccattcccaa tttctcagct tcttccttag tcatcaactt
    13861 gtgctcaata ggagcaaagg tgatgttgtc caatacagac atatgaggga agaggttgaa
    13921 gtgttggaat accatgccga tattttcacg gacgtggtca acattggttg ttttttcagt
    13981 taaatcatag ccgttcacag tgatgtgacc gctagtgact tcttctaaaa gattgaggct
    14041 acggaggaaa gttgacttac cagaacctga aggaccgatg atacaaacaa catctccttc
    14101 atagaactta gtcgtaattc cttttaggac ttcatttttt ccatagtgct tgtgtaaatc
    14161 atttacatca atttttaatt ttgccattaa cgaatcctct tttctaagcg tttcgctagt
    14221 ctagtcaaaa gtgtgataat tacaagatag aagatagcaa ggattgcata catcttgaaa
    14281 ctttggtagt tacgagcaat gataatctta ccagtttgga agagttcaac caaaccgata
    14341 gcagatacga tagttgtatc tttaagagcg ataacgaatt ggttgacaaa gtttggcaac
    14401 atcaatttag ttgcttgtgg caagataatc ttacgcatgg tttttccata agagataccc
    14461 aagcttcggc tggcttccat ttggccaact ggaacggcct gaataccacc acgaacgatt
    14521 tcagcgatat aagcagccgc attgagtgag agggcaatgg ttccagctac aaagtcgtta
    14581 attgggcttt gttggcctgt gatagactcg atgaagtttg gaattcccca gaagatgaag
    14641 gctgcaagaa tcatcaatgg aataccacga ataacgtcaa cgaaaatctc agagatgacg
    14701 cgaagagatt tgtatgggct aacgctaaac ataccgaaga taattccgat gacaatggca
    14761 atagcaaatg agataagagc tagagcaaga gtgataccaa gaccgctaag gagttgtttg
    14821 tagttgtttt gaagcaagcc ccagagcgtt gtttcgtcaa cagtacttgt tgaagcagtt
    14881 gaagattcgc tagctaggta tttgtcaaga atcttttgga attcaccgtt tgctttaagg
    14941 tttgcaagtc cgttgttgaa catttcaatc agttctggat ttgctccttt tttaacggca
    15001 aaggctgttt caccgattgg agttccagag attggagttt tcaatttttg accttggctg
    15061 atagaatatt tgagaacagg ttcatcatcc ataacggcat caatggcacc agtgtttaaa
    15121 ctgtcataca ttgaagaacc atcagcaaag gttttgattt tgtagccgta tttgctttga
    15181 ttttctgtta ggaaggtttg agaagcagtt ccgtttttaa caccgactgt ctttcctttt
    15241 agatcttcat aagaagcaat attacttgat tctttgacac caagaatggt attagcagtg
    15301 tagtatgatt ctgagaagtc aaaagttgcc ttacgagcat ctgtgacaga cataccagcg
    15361 atgataccat cggcttgacc agcttggaca gcactgatag cagcatcaaa accagggttg
    15421 gtgatttcaa tttcaaaacc ttggtcttta gcgattgcct taatcaattc catatcaata
    15481 ccagtgtatt ggttgcttga attttggaaa acaaaagggg caaaagaaga atcgctggca
    15541 atgatatatt tagccttaac aggaatagct tttaatcctg cgagagtagt tgtagttggc
    15601 actgctgaag atgatgaagc agtccatttc ttgataattt tatcaagact accatctttt
    15661 ttcatttcag acaaggcttg gttaaattca gtaaccaggt gctcgtattt acttcctttt
    15721 ttcacaccga aagcaaaact tcctacagct tcaccatcca tttcaatatg gaggtcttga
    15781 ccttggttaa tggcatattc gataacaggt ttgtcatcca tcatggcatc gatggcacca
    15841 gcactcaagc tgttgttcat taaatcacca gtgtcaaatg ttttaatagt aaagccatat
    15901 ttatctttga ttgtttcaag gaaacgttga gcggcagttc cgtttttaac accaacggtt
    15961 ttgccagtta attggtcgta cttgctaatt ttgtgtgact ttgtagtagc aatgacaact
    16021 tttgtatcat agtaagtatc agacatggtg aagacttttt cacgttcttt agtctttgtc
    16081 atccctgcca tgatagcgtc ggcttgccca gcttgaaccg cattgactgc tgcgtcaaat
    16141 ccaggatagg acatctgaat gttccagcct ttaatctcag cgactttgtt aataatgtca
    16201 acatcaattc ctttataagt ttgatctgaa tctttaaact caaaaggtgc ataggcggta
    16261 tcagaaacaa tcttaatcgt ttctgctttg gcaataccta atgagaaaat tgggaataaa
    16321 attagcaaaa atgctagaaa ttttttcttc atttttagtc tccttttccg aatattctct
    16381 cattatatca gaaaaagtaa aaaaaggcaa a
//
LOCUS       DAVKWS010000042        16166 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKWS010000042 DAVKWS010000000
VERSION     DAVKWS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.042"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            108..2090
                     /locus_tag="VW852_001508"
     CDS             108..2090
                     /locus_tag="VW852_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0272631.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKAEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAVVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGGITDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2391..7694
                     /locus_tag="VW852_001509"
     CDS             2391..7694
                     /locus_tag="VW852_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0272632.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKSKDIDFGKIIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPIVEKPEFKLNSLASEQGKTPDYKQEIA
                     RPETPAQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7861..10020)
                     /locus_tag="VW852_001510"
     CDS             complement(7861..10020)
                     /locus_tag="VW852_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV0272633.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAINTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTEMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIEKHIKNTGYVAPDETFVGYTRKYSMAVWTGYTNRLTPIMGDGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10017..10613)
                     /gene="recU"
                     /locus_tag="VW852_001511"
     CDS             complement(10017..10613)
                     /gene="recU"
                     /locus_tag="VW852_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV0272634.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10679..11206
                     /locus_tag="VW852_001512"
     CDS             10679..11206
                     /locus_tag="VW852_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV0272635.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11276..11605
                     /gene="gpsB"
                     /locus_tag="VW852_001513"
     CDS             11276..11605
                     /gene="gpsB"
                     /locus_tag="VW852_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV0272636.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11626..12006
                     /gene="rnpB"
                     /locus_tag="VW852_001514"
     ncRNA           11626..12006
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW852_001514"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12091..13248
                     /locus_tag="VW852_001515"
     CDS             12091..13248
                     /locus_tag="VW852_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV0272637.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13261..14631
                     /gene="mapZ"
                     /locus_tag="VW852_001516"
     CDS             13261..14631
                     /gene="mapZ"
                     /locus_tag="VW852_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV0272638.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEELVETQSLDDLIQEMREAVEESEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     EPKPSSSNETRSSRSEVNMGLSSEGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            14707..16131
                     /gene="gndA"
                     /locus_tag="VW852_001517"
     CDS             14707..16131
                     /gene="gndA"
                     /locus_tag="VW852_001517"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV0272639.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
BASE COUNT         4991 a         3008 c         3657 g         4510 t
ORIGIN      
        1 aaagtatttt tcagaattta ccaaattaat taaaaaattc agaaaattat attgacatct
       61 ctctgaaaag agtctataat agagagaaag ttttaaagga gaagatgatg aaaagttcaa
      121 aactatttgc ccttgcgggt gtaaccttat tggcggcgac tactttagct gcatgctctg
      181 gatcaggttc aagcactaaa gctgagaaga cattctcata catttatgag acagaccctg
      241 ataacctcaa ctatttgaca actgctaagg ctgcgacagc aaatattacc agtaacgtgg
      301 ttgatggttt gctagaaaat gatcgctacg ggaactttgt gccgtctatg gctgaggatt
      361 ggtctgtatc caaggatgga ttgacttaca cttatactat ccgtaaggat gcaaaatggt
      421 atacttctga aggtgaagaa tacgcggtag tcaaagctca agactttgta acaggactaa
      481 aatatgctgc tgataaaaaa tcagatgctc tttaccttgt tcaagaatca atcaaagggt
      541 tggatgccta tgtaaaaggg ggaatcacag atttctcaca agtaggaatt aaggctctgg
      601 atgatcagac agttcagtac actttgaaca aaccagaaag cttctggaat tctaagacaa
      661 ccatgggtgt gcttgcgcca gttaatgaag agtttttgaa ttcaaaaggg gatgattttg
      721 ccaaagctac ggatccaagt agtctcttgt ataacggtcc ttatttgttg aaatccattg
      781 tgactaaatc ttctgttgaa tttgcgaaaa atccgaacta ctgggataag gacaatgtgc
      841 atgttgacaa agttaaattg tcattctggg atggtcaaga taccagcaaa cctgcagaaa
      901 actttaaaga tggtagcctt acagcagctc gtctctatcc aacaagtgca agtttcgcag
      961 agcttgagaa gagtatgaag gacaatattg tctatactca acaagactct attacgtatc
     1021 tagttggtac aaatattgac cgtcagtcct ataaatacac atctaagacc agcgacgaac
     1081 aaaaggcatc gactaaaaag gctctcttaa acaaggattt ccgtcaggct attgcctttg
     1141 gttttgatcg tacagcctat gcctctcagt taaatggaca aactggagca agcaaaatct
     1201 tacgtaatat ctttgttcca ccaacatttg ttcaagcaga tggtaaaaac tttggcgata
     1261 tggtcaaaga gaaattggtc acttatgggg atgaatggaa ggatgttaat cttgcagatt
     1321 ctcaggatgg tctttacaat ccagaaaaag ccaaggctga atttgctaaa gctaaatcag
     1381 ccttacaagc agaaggtgtg actttcccaa ttcatttgga tatgccagtt gaccagacag
     1441 caactacaaa agttcagcgc gtccaatcta tgaaacaatc cttggaagca actttaggag
     1501 ctgataatgt cattattgat atccaacaac tacaaaaaga cgaagtaaac aatattacat
     1561 attttgctga aaatgctgct ggcgaagact gggatttatc agataatgtc ggttggggtc
     1621 cagactttgc cgatccatca acctaccttg atatcatcaa accatctgta ggagaaagta
     1681 ctaaaacata tttagggttt gactcagggg aagataatgt agctgctaaa aaagtaggtc
     1741 tatatgacta cgaaaaattg gttactgagg ctggtgatga ggctacagat gttgctaaac
     1801 gctatgataa atacgctgca gcccaagctt ggttgacaga tagtgctttg attattccaa
     1861 ctacatctcg tacagggcgt ccaatcttgt ctaagatggt accatttaca ataccatttg
     1921 cattgtcagg aaataaaggc acaagtgaac cagtcttgta taaatacttg gaacttcaag
     1981 acaaggcagt cactgtagat gaataccaaa aagctcagga aaaatggatg aaagaaaaag
     2041 aagagtctaa taaaaaggct caagaagatc tcgcaaaaca tgtgaaataa ctgttgcaaa
     2101 atataagaaa ggatttagta tttctcttga atgctgaatc cttttttaca tttgtaaaga
     2161 aagattctaa atgtactgcc ccccaaaagt tggagcctct ttttgtcaga atagagaaaa
     2221 tttttgttaa ttttacttgt ttcctattgc tttctcagct attatttgtt atattaaaag
     2281 tataattatt ttttatttat cagggttaag cattgcactt tcagaggaag gagtattttt
     2341 taaaaaggaa atgtaaacgc ttgctaaaaa atgaaaggat ttagaagttt atgaataaag
     2401 gattatttga aaaacgttgt aaatatagta ttcggaaatt ttcattaggt gttgcttctg
     2461 ttatgattgg agctgcattc tttgggacaa gtccggttct tgcagatagc gtgcagtctg
     2521 gttccacggc gaacttacca gctgatttag ctactgctct tgcaacagca aaagagaatg
     2581 atggacgtga ttttgaagcg cctaaggtgg gagaagacca aggttctcca gaagttacag
     2641 atggacctaa gacagaagaa gaactattag cacttgaaaa agaaaaaccg gctgaagaaa
     2701 aaccaaaaga ggataaacct gcagctgcta aacctgaaac acctaagacg gtaacccctg
     2761 aatggcaaac ggtagagaaa aaagaacaaa agggaacagt cactatccga gaagaaaaag
     2821 gtgtccgcta caaccaacta tcctcaactg ctcaaaatga taacgcagga aaaccagccc
     2881 tgtttgaaaa gaagggcttg accgttgatg ccaatggaaa tgcaactgtt gatttaacct
     2941 tcaaagatga ttctgaaaag ggcaaatcac gctttggtgt cttcctgaaa tttaaagata
     3001 ccaataataa tgtttttgtc ggttatgaca aggatggctg gttctgggag tataaatctc
     3061 caacaactag cacttggtat agaggtagtc gtgttgctgc tcctgaaaca ggatcaacaa
     3121 accgtctctc tatcactctc aagtcagacg gtcagctaaa tgccagcaat aacgatgtca
     3181 atctctttga cacagtgact ctaccagctg cggtcaatga ccatcttaaa aatgagaaga
     3241 agatccttct caaggcgggc tcttatggca atgaccgtac ggttgtcagc gttaaaacag
     3301 acaatcaaga aggcgtaaaa gcggatgata ctcctgccca gaaagaaaca ggtccggttg
     3361 ttgacgatag caaggtgact tatgatacga tccagtctaa ggtacttaag gcagtaattg
     3421 accaagcctt ccctcgtgtc aaggaataca gcttgaacgg gcatactttg ccaggacagg
     3481 tgcaacagtt caaccaagtc tttatcaata accaccgaat cacccctgaa gtcacttata
     3541 agaaaatcaa tgagacaaca gcagagtact tgatgaagct tcgcgatgat gctcacttaa
     3601 tcaatgcgga aatgacagta cgcctgcaag ttgtggacaa tcaattgcac tttgatgtga
     3661 ccaagattgt caaccacaat caagtcactc caggtcaaaa gattgatgac gaaagaaaac
     3721 tactttcttc tattagtttc ctcggcaatg ctttagtctc tgtttctagt gatcaaactg
     3781 gtgctaagtt tgatggggca accatgtcaa acaatacgca tgtcagcgga gatgatcata
     3841 tcgatgtaac caatccaatg aaagacctag ccaagggtta catgtatgga tttgtttcta
     3901 cagataagct tgctgctggt gtttggagta actctcaaaa cagctatggt ggtggttcga
     3961 atgactggac tcgtttgaca gcttataaag aaacagtcgg aaatgccaac tatgtaggaa
     4021 tccacagctc tgaatggcaa tgggaaaaag cttataaggg cattgttttc ccagaataca
     4081 cgaaggaact tccaagggct aaggttgtta tcactgaaga tgccaatgca gacaagaaag
     4141 tcgattggca ggatggtgcc attgcttatc gtagcattat gaacaaccct caaggttggg
     4201 aaaaagttaa ggatatcaca gcttaccgta tcgcgatgaa ctttggttct caagcacaaa
     4261 acccattcct tatgaccttg gatggtatca agaaaatcaa tctccacaca gatggtcttg
     4321 ggcaaggtgt tctccttaaa ggatatggta gcgaaggcca tgactctggt cacttgaact
     4381 atgctgatat tggtaagcgt atcggtggtg tcgaagactt caagacccta attgagaagg
     4441 ctaagaaata tggagctcat ctaggtatcc acgttaacgc ttcagaaact tatcctgagt
     4501 ctaaatactt caatgaaaaa attctccgta agaatccaga tggaagttat agctatggtt
     4561 ggaactggct agatcaaggt atcaacattg atgctgccta tgacctagct catggtcgtt
     4621 tggcacgttg ggaagatttg aagaaaaaac ttggtgacgg tctcgacttt atctatgtgg
     4681 acgtttgggg taatggtcaa tcaggtgata acggtgcctg ggctacccac gttcttgcta
     4741 aagaaattaa caaacaaggc tggcgctttg cgatcgagtg gggccatggt ggtgagtacg
     4801 actctacctt ccatcactgg gcagctgact tgacctacgg tggctacacc aataaaggta
     4861 tcaacagtgc catcactcgc tttatccgta accaccaaaa agatgcttgg gttggggact
     4921 acagaagtta tggtggtgca gccaactatc cactgctagg tggctacagc atgaaagact
     4981 ttgaaggctg gcaaggaaga agtgactaca atggctacgt gactaacctc tttgctcatg
     5041 acgtcatgac taagtacttc caacacttca ctgtaagtaa atgggaaaat ggtacaccgg
     5101 tgactatgac cgataacggt agcacctata aatggactcc agaaatgcga gtggagttgg
     5161 tagatgctga caataataaa gtagttgtaa ctcgtaagtc aaatgatgtc aatagcccac
     5221 aatatcgcga acgtacagta acgctcaacg gacgtgtcat ccaagatggt tcagcttact
     5281 tgactccttg gaactgggat gcaaatggta agaaactttc tactgagaag gaaaagatgt
     5341 actacttcaa tacgcaggcc ggtacaacaa cttggaccct tccaagcgat tgggcaaaga
     5401 gcaaggttta cctttacaag ctaactgacc aaggtaagac agaagagcaa gaactaactg
     5461 taaaagatgg taaaattacc ctagatcttc tagcaaatca accatacgtt ctctatcgtt
     5521 cgaaacaaac caatcctgaa atgtcatgga gtgaaggcat gcacatctat gaccaaggat
     5581 ttaatagcgg taccttgaaa cattggacca tttcaggcga tgcttctaag gcagaaattg
     5641 tcaagtctca aggggcaaac gatatgcttc gtattcaagg aaacaaagaa aaagttagtc
     5701 tcactcagaa attaactggc ttgaaaccaa ataccaagta tgccgtttat gtcggtgtcg
     5761 ataaccgtag taatgccaag gcgagcatca ctgtaaacac tggtgaaaaa gaagtgacta
     5821 cttataccaa taagtctctc gccctcaact atgtaaaagc ctacgcccac aatacacgtc
     5881 gtaacaatgc tacagttgac gatacaagtt acttccaaaa catgtacgcc ttctttacaa
     5941 ctggatcgga cgtatcaaat gttactctga cattgagtcg tgaagctggt gatcaagcaa
     6001 cttactttga tgaaattcgt acctttgaaa acaattcaag catgtacgga gacaagcatg
     6061 atacaggtaa aggcaccttc aagcaagact ttgaaaatgt tgctcagggt atcttcccat
     6121 ttgtagtggg tggtgtcgaa ggtgtcgaag acaaccgcac tcacttgtct gaaaaacatg
     6181 atccatatac acaacgtggt tggaacggta agaaagttga tgatgttatc gaaggaaatt
     6241 ggtcactcaa gacaaatgga ctagtgagcc gtcgtaactt ggtttaccaa actattccgc
     6301 aaaacttccg ttttgaagca ggtaagacct accgtgtaac ctttgaatac gaagcaggtt
     6361 cagacaatac ctatgctttt gtagtcggta agggagaatt ccagtcaggt cgtcgtggta
     6421 ctcaagcaag caacttggaa atgcatgaat tgccaaatac ttggacagat tctaagaaag
     6481 ccaagaaggc aaccttcctc gtgacaggtg cagaaacagg ggatacttgg gtaggtatct
     6541 actcaactgg aaatgcaagt aatactcgtg gtgattctgg tggaaatgcc aacttccgtg
     6601 gttataacga cttcatgatg gataatcttc aaatcgaaga aattacccta acaggtaaga
     6661 tgttgacaga aaatgctctg aagaactact tgccaacggt tgccatgact aactacacca
     6721 aagagtctat ggatgctttg aaagaggcgg tctttaacct cagtcaggcc gatgatgata
     6781 tcagtgtgga agaagcgcgt gcagagattg ctaagattga agccttgaag aatgctttgg
     6841 ttcagaagaa aacggctttg gtagcagatg actttgcaag tcttacagct cctgctcagg
     6901 ctcaagaagg tcttgcaaat gcctttgatg gaaacttatc tagtttatgg catacatcat
     6961 ggggcggagg agatgtaggc aagcctgcaa ccatggtctt gaaagaagca actgaaatca
     7021 ctggacttcg ttatgttcca cgtggatcag gttcaaatgg taacttgcgt gatgtgaaac
     7081 ttgttgtgac agatgagtct ggcaaggagc atacctttac tgcaactgat tggccagata
     7141 acaataagtc aaaagatatt gactttggta agataattaa ggctaagaaa attgtcctta
     7201 caggtactaa gacttacgga gatggtggcg ataaatacca atctgcagcg gaactcatct
     7261 ttactcgtcc acaggtagca gaaacacctc ttgacttgtc aggctatgaa gcggctttgg
     7321 ctaaggctca gaaattaaca gacaaagaca atcaagagga agtagctagc gttcaggcaa
     7381 gcatgaaata tgcgacggat aaccatctct tgacggaaag aatggtggaa tactttgcag
     7441 attatctcaa ccaattaaaa gattctgcta cgaaaccaga tgctccaatt gtagagaaac
     7501 ctgagtttaa acttaactct ttagcttccg agcaaggtaa gacgccagat tataagcaag
     7561 aaatagctag accagaaaca cctgcacaaa tcttgccagc aacaggtgag agtcaatctg
     7621 acacagccct cttcctagca ggtgttagcc tagccctatc tgctctcttt gtagtaaaaa
     7681 cgaagaaaga ctagtattta gtaaaacctc ttaacaagat tacggaagca gtctctatct
     7741 tttccaatga ggtttatagt acagaaaaag cctgagaaga tgtcttctca ggcttttgtt
     7801 aagcacataa atacaatagt gctatgacaa aatcacccag aaaaatctgg gtgataaatg
     7861 ttatggttgt gctggttgag gattctgatt ttgttgatca ggggttgtat ttgattgttg
     7921 cgtattattg ttaggattgg tagtcgtact attatttgtg cttggagtgg ttgagctaga
     7981 ctgtgaagtt gaactatctg atgatgagct tgaactttca gttgatgggg gttgttgtgg
     8041 agcaggtgag ttccacgtag aacgagcacc atttttaaat acgaattctc catttctgta
     8101 gagcccctct ggtatattcc aatcctctgg attgcttcct tcagacagat aggtcatcat
     8161 agagcggtaa actttggcag caactgtgag accatccccc ataattggtg tgagacggtt
     8221 tgtgtaacct gtccatacag ccatagaata cttacgagta taaccaacaa acgtttcatc
     8281 tggagctaca tagccagtgt ttttgatgtg tttttcaatt tcttcatctg tatagttaga
     8341 ggtacctgtt ttaccagctt gaggaagcca agcaagatag gcatttcgtc cagttccata
     8401 actcaagact gttttcatca tttcggtcat catataggct gtcgtttcct tcatggcacg
     8461 agttccgaca ttagagaact ctttttcact cccatcacta aagacgactt tatggatata
     8521 cattggttta tagtaagttc caccatttgc aaaggcagcg taagcagcag ccatcttttc
     8581 actacttgct ccatattttt tgtctgattc ggttgtgtta cttgaaatgg catttgagta
     8641 gtgaatactt gggtagtcga ttccgagacc atttaggaaa gtcttggcgc ggttgagtcc
     8701 gaccttgttt agagtttcca cggctgggac gtttcgcgat tgttgcaggg cgtattgcaa
     8761 ggtgatgttg ccaaagtagc ccctatccca gttataaaca ggagtatttg tcccagggta
     8821 gttatagggc tcatcgtgaa cgatagtagc agttgaatcg tagacaccgt actccaaggc
     8881 aggagcatag tctgtgatcg gtttcatagt tgatccccag tcgcggtttg tttctactgc
     8941 ttggttaatt ccgaaggaaa cattacttga ctgatggcgt gctcctagct gggcaatgac
     9001 tttaccgtta gaaacatcaa caatggtaga agcgacttgc aattcatcgt ctggataggc
     9061 aacgtattcg tctgtattgt aaatatccca cagatgtttt tgagcttctt ggtctacatt
     9121 tgtgtagaca tccatcccag ttgtgagcag gttatagcct gtttcttctt caacttgatt
     9181 gatgacttcc ttgaggtaat tatccatgta agcagggtaa ttacttgctg atttgagact
     9241 ttgtagtcca tcagtaattg gtgtattgat tgctttctca tactgttcag cagagatgta
     9301 gccttgattt ttcatttcag ataagaccaa gtttcggcgg tcttgggctg cttctggatg
     9361 tgaatagggg tcatattggt ttggtgcctg aggcattcca gccagcaagg ctaactgagg
     9421 taaacttaaa ttattgaggt ctttaccata gtagttttga gctgctgtct gcattccata
     9481 gttcccatta gacatgtaga ccttatttat atagtaggtc aagatttctt gcttggttgc
     9541 tttttgttct aactgaatcg ctaaccaagc ttcctgagcc ttacgagaaa tagtctggtc
     9601 ggaagtcgaa gttgaaaagt aagtcaactt aatcaactgt tgggtgagag ttgatccacc
     9661 ttggagggaa ttgctttgca gattgcgcaa gaaagctccc aggatacgga tggtatcaat
     9721 ccccctgtgg tcgaagaagc gatggtcttc gatagaaacg attgccttaa ccaaatctgt
     9781 gggaatatca ttagcttggg cattgacgcg gcgttcagaa cccaagtcag caatgagttg
     9841 atttttattg tcgtagattt tactagaagt tgttgcaact agtttactct cggataggct
     9901 aggagccttg ctaacgtagt agaaaaaaac tcctccgcct aagacaatgg ctgcgataac
     9961 caagcttaag aagctaatgc tcagatactt gattaggcgc agaatcgttg gtttgttcat
    10021 cttgttttac cacctaataa atgttctttg ataacattga gataaggaat ttgagggaag
    10081 gcaccagcct tgatttcata tccatattct cgaatatatt caagtggcat tgatttttgt
    10141 cccttatctt gatgatagaa gcgaatcaaa tcgaatgccg gcaataagta ggtttcttgc
    10201 tgagaagaaa agtgaagaag gacaaagcag attccttgtt gggcaaggac ttgttccata
    10261 tgctgaatct gatgtggatg aaaatttttc atcggaatcg cacgtttttg ttttgtttcc
    10321 ttgacttcaa agtcgatgta atatccatta taaacgccag aatagtccgt cgttgaagct
    10381 tgtcgaaaat aggcttcaac aatcttggca cgacttcgtt gtggatagtc cacttgtacg
    10441 atttgaatag gagttggttt cttatgtata acagccaagc cctgagacaa atagtagtcg
    10501 ttggtagcat tgatcatctt ttcaaaagac attcctcgat ttgcgaaatt tttgggttga
    10561 gaaagagatg tttgtctttt ttgtgatgaa actttatgtg gatagttgac cataattctc
    10621 cttattggta caataacatc actttattat accataattt gcacagaaag ggttaaaaat
    10681 ggctacagct ttggttttag gctattctgc ttttgatttg ggtttatttt ctgataagga
    10741 tcctcgtctt aaattgatta aaaaagcgat acgtaaagat ttagaggcta tggcagcaga
    10801 tggggtgtct tggcttgtgt ttacagggag tttgggtttt gaatattggg ttttagaggt
    10861 tgctcaggag atgaaaaccg aatacggttt ccagttggcc accatttttg cttttgaaac
    10921 ccatggggaa aattggaatg aaggcaatca gatgaaactg agtcgcttta agcaggtaga
    10981 ctttgtcaaa tatgcctatc ctcgctatga acacaagggg cagttaagag attaccagca
    11041 gtttttgctg gaaaacacga ctagttccta tcttttttat gatgaagaaa atgaaacgaa
    11101 actagcttat ttttaccaaa agatgaaaaa tcaagaggac tattttataa agagattaac
    11161 atttgatcaa ttaaatgaac ttgctgaaaa tttttccgaa aattgaagct ttgaccttga
    11221 tttttgtttg ccttttttta tataataata ctagcaagcg agaatggaga gagacatggc
    11281 aagtattatt ttttcagcga aagatatttt tgaacaagag tttggacgtg aagtccgtgg
    11341 ctataataaa gtagaagttg acgagttttt agacgatgtc atcaaggact atgaaaccta
    11401 tgctgccttg gtcaagtcac ttcgtcagga aattgctgat ttgaaggaag aattaactcg
    11461 taaaccgaaa ccttcaccag ttcaagcaga accccttgaa gcggcaatta caagttctat
    11521 gacgaatttt gatattttga aacgcctgaa tagattggaa aaagaagttt ttggtaaaca
    11581 aattttagat aactcagatt tttaagtagt tatttgagat gtgcaatttt tggataatcg
    11641 cgtgaggaga attgcttctc atgaggaaag tccatgctag cacaggctgt gatgcctgta
    11701 gtgtttgtgc taggcgaaac cataagccta gggacgagaa atcgttacgg cagttgaaat
    11761 ggctaagtcc ttggataggc cagagtaggc ttgaaagtgc cacagtgacg gagtctttct
    11821 ggaaacagag agagtggaac gcggtaaacc cctcaagcta gcaacccaaa ttttggtcgg
    11881 ggcatggagt acgcggaaac gaacgtagta ttctgactgc tatcagctag agctgttagt
    11941 ggtagacaga tgattatcga aggaagtggt cctagtcact tctggaacaa aacatggctt
    12001 atagaaaatt gcatataggt tggggctgag aaattttctc aacctcattt tttaaagtgg
    12061 acatatagaa aggtcttgca agactgtaac atgaaaaaag aatttaattt aattgcaact
    12121 gtggcagcag ggcttgaggc tgtcgttggt cgtgaagtgc gagagttggg ctacgattgt
    12181 caggttgaaa atggacgtgt tcgttttcaa ggagacgtga gagctattat cgaaaccaac
    12241 ctttggcttc gggcagcaga tcgtatcaaa attatcgtag gaacgttccc agctaagact
    12301 tttgaagagc tatttcaggg agttttcgct ttagattggg aaaattattt accacttgga
    12361 gctcggttcc cgatttcaaa agctaaatgt gttaagtcca aacttcacaa tgagcccagt
    12421 gttcaggcta tttctaagaa agctgttgtc aagaaattgc agaaacacta tgctcgccca
    12481 gaaggggttc ctctgatgga gaatggtcca gagtttaaga ttgaggtctc tattctcaaa
    12541 gatgtggcaa ctgtcatgat tgatacgacc gggtctagcc tctttaaacg tggttatcgt
    12601 accgaaaaag gtggcgctcc tatcaaggaa aatatggcag cagccatttt acaactttct
    12661 aactggtatc cagacaagcc tttgattgat ccgacctgtg gttcggggac tttctgtatt
    12721 gaggcagtta tgattgctag aaagatggcg ccaggtcttc gtcgctcttt tgcatttgag
    12781 gaatggaact ggatcagcga tcgcttgatt caagaagtgc gcacagaagc ggctaaaaaa
    12841 gtagaccgtg agcttgagct ggatatcatg ggctgtgata ttgatgctcg catggtggaa
    12901 attgctaagg ccaatgctca ggcagctggt gttgcaggag acattacttt taagcagatg
    12961 cgcgtgcagg atttacgttc cgataaaatc aatggagtaa tcatttccaa tccgccttat
    13021 ggtgaacgtt tgtcagatga tgcaggggtg accaagctct atgctgagat ggggcaagta
    13081 tttgcaccgc tgaaaacttg gagcaaattt atcctgacta gtgatgaagc ttttgaaagc
    13141 aagtatggta gccaagcaga taagaagcgt aagttataca acggaacctt gaaagtggat
    13201 ctgtatcaat attttggtca gcgtgtcaaa cggcaagagg taaaatagaa agggatactc
    13261 atgagtaaaa aaagacgaaa tcgtcataaa aaagaaggtc aagaaccgca atttgatttt
    13321 gatgaagcaa aagagctaac agttggtcaa gctattcgta aaaatgaaga agtggaagca
    13381 ggagtcttgc ctgaggattc cattttggac aagtatgtta agcaacacag agatgaaatt
    13441 gaggcggata agtttgcgac tcgtcaatac aaaaaagagg agttagttga aactcagagt
    13501 ctggatgatt taattcaaga gatgcgtgag gctgtagagg agtcagaagc ttcttcggag
    13561 gaagttccat cttctgaaga catcttacta cccttgcctc tggacgatga ggagcagggc
    13621 ttggatcctc tattgttaga tgatgaaaat ccaacagaaa tgactgaaga agtggaagag
    13681 gagcaaaacc tttctcgtct ggatcaagag gactcagaaa agaaaagtaa aaaaggcttt
    13741 attttgaccg ttttggcgct tgtatcagta attatttgtg tcagtgctta ttatgtctac
    13801 cgtcaagtgg ctcgttcgac caaggaaatt gaaacttctc aatcaactac agccaatcaa
    13861 tcggatgtgg atgattttaa tacactttat gacgcctttt acacagatag caataaaacg
    13921 gctttgaaaa atagccagtt tgataaactg agtcaactca aaaccttgct tgataaactg
    13981 gaaggtagtc gtgaacatac gcttgccaaa tctaaatatg atagtctagc aacgcaaatc
    14041 aaggctattc aagatgtcaa tgcacaattt gaaaaatcag ctattgtgga tggtgtgttg
    14101 gataccaatg ccaaagccaa atcggatgct aaatttacgg atattaaaac tggaaatacg
    14161 gagcttgata aagtgctaga taaggctatt agccttggta agagccagca aacaagtact
    14221 tctagctcaa gttcaagtca agcaagctca aatacgacaa gtgagccaaa accaagtagt
    14281 tcaaatgaga ctagaagtag tcgcagtgaa gtcaatatgg gtctctcgag tgaaggggtt
    14341 gctgttcaaa gaagtgccag tcgtgttgcc tataatcagt ctgctattga tgatagtaat
    14401 aactctgcct gggattttgc ggatggtgtc ttggaacaaa ttctagcgac ttcacgttca
    14461 cgtggctata tcactggaga ccaatatatc cttgaacgtg tcaatatcgt taacggcaat
    14521 ggttattaca acctctataa gccagatgga acctatctct ttacccttaa ctgtaagaca
    14581 ggctactttg tcggaaatgg cgctggtcat gcagatgact tggactacta agcagtcgtt
    14641 acaaaattct ttcttttcaa aagtaaaaat gataaaataa aacaaatgaa acaagaggag
    14701 tgtcaaatga caaaagctaa ctttggtgtc gtaggtatgg ccgtaatggg tcgtaacctt
    14761 gcccttaata ttgaatcacg tggttacaca gttgctatct acaaccgtag taaagaaaaa
    14821 acggaagatg tgattgcttg ccatcctgaa aagaattttg taccaagcta tgacgttgaa
    14881 agttttgtaa actcaatcga aaaacctcgt cgtatcatgc tgatggttca agctggacct
    14941 ggtacagatg ctactatcca agcccttctt ccacaccttg acaagggtga tatcttgatt
    15001 gacggaggaa acactttcta caaagatacc atccgtcgta atgaagaatt ggcaaactca
    15061 ggtatcaact ttatcggtac tggagtttct ggtggtgaaa aaggtgccct tgaaggtcct
    15121 tctatcatgc ctggtggaca aaaagaagcc tacgaattgg ttgcggatgt tcttgaagaa
    15181 atctcagcta aagcaccaga agatggcaaa ccatgtgtga cttacatcgg tcctgatgga
    15241 gctggtcact atgtgaaaat ggttcacaat ggtatcgagt acggtgatat gcaattgatc
    15301 gcagaaagct atgacttgat gcaacacttg ctaggccttt ctgcagagga tatggctgaa
    15361 atctttactg agtggaacaa gggtgaattg gatagctact tgattgaaat cacagctgat
    15421 atcttgagcc gtaaagacga tgaaggccaa gatggaccaa tcgtagacta catccttgat
    15481 gctgcaggta acaagggaac tggtaaatgg actagccaat catctcttga ccttggtgta
    15541 ccattgtcac tgattactga gtcagtgttt gcacgctaca tttcaactta caaagaagaa
    15601 cgtgtacatg ctagcaaggt gcttccaaaa ccagccgcct tcaactttga aggagacaag
    15661 gctgaattga ttgaaaaaat ccgtcaagcc ctttacttct caaaaatcat ttcatacgca
    15721 caaggatttg ctcaattgcg tgtagcctct aaagaaaaca actggaactt gccatttgca
    15781 gatatcgcat ctatctggcg tgatggctgt atcatccgtt ctcgtttctt gcaaaagatt
    15841 acagatgctt acaaccgtga tgcagacctt gccaaccttc ttttggatga atacttcttg
    15901 gatgttactg ctaagtatca acaagcagtg cgtgatatcg tagctcttgc ggttcaagct
    15961 ggtgtgccag tgccaacttt ctcagcagct attacttact ttgatagcta ccgttcagct
    16021 gaccttccag ctaacttgat ccaagcacaa cgtgactact ttggtgccca cacttaccaa
    16081 cgtaaagaca aagaaggaac cttccactac tcttggtatg acgaaaaata agtaggtcag
    16141 ccatggggaa acggatttta ttactt
//
LOCUS       DAVKWS010000043        15462 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKWS010000043 DAVKWS010000000
VERSION     DAVKWS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.043"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(134..253)
                     /locus_tag="VW852_001518"
     CDS             complement(134..253)
                     /locus_tag="VW852_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV0272640.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(357..506)
                     /gene="blpO"
                     /locus_tag="VW852_001519"
     CDS             complement(357..506)
                     /gene="blpO"
                     /locus_tag="VW852_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV0272641.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(750..953)
                     /gene="blpN"
                     /locus_tag="VW852_001520"
     CDS             complement(750..953)
                     /gene="blpN"
                     /locus_tag="VW852_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV0272642.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(969..1223)
                     /gene="blpM"
                     /locus_tag="VW852_001521"
     CDS             complement(969..1223)
                     /gene="blpM"
                     /locus_tag="VW852_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV0272643.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1373..2077)
                     /locus_tag="VW852_001522"
                     /pseudo
     CDS             complement(1373..2077)
                     /locus_tag="VW852_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(3165..3368)
                     /locus_tag="VW852_001523"
     CDS             complement(3165..3368)
                     /locus_tag="VW852_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pncT"
                     /protein_id="HEV0272644.1"
                     /translation="MEKIDYITLNEVELETISGGDDCFIGDIGCIGWGLLKSIGGMIK
                     PAPYVPPVCIPKSSWNPAPPVPC"
     gene            complement(3686..3889)
                     /locus_tag="VW852_001524"
     CDS             complement(3686..3889)
                     /locus_tag="VW852_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV0272645.1"
                     /translation="MNTKMMSQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIV
                     DTAIDDFTNQCRKNPHQWFCVRV"
     gene            4170..6323
                     /gene="blpA"
                     /locus_tag="VW852_001525"
     CDS             4170..6323
                     /gene="blpA"
                     /locus_tag="VW852_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEV0272646.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            6334..7695
                     /locus_tag="VW852_001526"
     CDS             6334..7695
                     /locus_tag="VW852_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV0272647.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            7752..7880
                     /gene="blpC"
                     /locus_tag="VW852_001527"
     CDS             7752..7880
                     /gene="blpC"
                     /locus_tag="VW852_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV0272648.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(7951..9291)
                     /locus_tag="VW852_001528"
     CDS             complement(7951..9291)
                     /locus_tag="VW852_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0272649.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLAFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(9305..10042)
                     /locus_tag="VW852_001529"
     CDS             complement(9305..10042)
                     /locus_tag="VW852_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272650.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(10047..10385)
                     /locus_tag="VW852_001530"
     CDS             complement(10047..10385)
                     /locus_tag="VW852_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272651.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            10662..10991
                     /locus_tag="VW852_001531"
     CDS             10662..10991
                     /locus_tag="VW852_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV0272652.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(11159..12208)
                     /locus_tag="VW852_001532"
     CDS             complement(11159..12208)
                     /locus_tag="VW852_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272653.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(12205..12936)
                     /locus_tag="VW852_001533"
     CDS             complement(12205..12936)
                     /locus_tag="VW852_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272654.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLTPYSGSININGLTLQEDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVTFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            13004..13414
                     /locus_tag="VW852_001534"
     CDS             13004..13414
                     /locus_tag="VW852_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0272655.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            13424..13711
                     /locus_tag="VW852_001535"
     CDS             13424..13711
                     /locus_tag="VW852_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272656.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(14078..15214)
                     /gene="dnaJ"
                     /locus_tag="VW852_001536"
     CDS             complement(14078..15214)
                     /gene="dnaJ"
                     /locus_tag="VW852_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV0272657.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         5026 a         3288 c         2579 g         4569 t
ORIGIN      
        1 tttatattgt attgatacca acttgtttgt agacttttca tcctgctatc acatatcatt
       61 ttgacaggcg aaacaatatt aaagaaactc ccctgtaaat taagctagca aatacagggg
      121 agaaatttat tttttagaga gtactatccg tatccttttt ggaagatttt gaaaatattt
      181 ttctaattaa gtcatccata taaggaccaa atataccaac tactaaacca ataataaaac
      241 ttttaaaatc cataattacc accaacatat tgctgcatag gctacacctc caagtatagc
      301 tccacctgca gcaccagtta cacctattcc tatagcaaat ggtcccaata gaaatgtcaa
      361 accgttgttg cacacccatc aattgcgcca tatgcaaccc ctgctgcaca actaattttt
      421 cttccccaat caatatctcc accttcaacg caagcaagca tttcattatc cataactgca
      481 aattgtgaca tcatttttgt atccatatag tatatcactt ttcagttacg gaacaagttt
      541 aatataaaaa ttatcaaaaa aacataggca ataaagagaa aaattaattt atcatagatt
      601 agaaataata tgacaaaaca attcaatgat gttaattcaa tagtcttttg ttttttatcg
      661 gagatactta tggatagata aataagatag gtttgaaaag cgaagagaat aataaagaat
      721 atagccttca taaaatttag ctttcatttt tatgatgtag cggtataggc taaatatcca
      781 caaaccactg ctcctccaat tcctcctatt gcagcgcccc atggtcctag aagtctccca
      841 tatttcactc cacccgctgc acaacctaaa gcagcaacta cagctgctcc tccggaatta
      901 cctccataaa cctcactcag cattgtttca tttatattac aataagtatt catacaagtc
      961 tccttttatt aaaatccacc cgttgcccct gttactcctg cccaaagatc cacaccaaat
     1021 ttagctccta tgtatccaca tgctcccata aatggtgctc caacaccact cgcagcacaa
     1081 atagctgtcc ctagccccca gccaccaaaa gcagcaccac caccttctaa gacattagtt
     1141 tgccaattat tcttgcctcc ttcaatacta gataacatag ttatatccat ttcatgaaat
     1201 tgttccataa tttttgtatc catgacaaat actctttttt atttttaatt tttgtcttgt
     1261 tgtaactttg acaagtttag tatatcatcg ttttttaaaa tttttcatcc agatcttgaa
     1321 tagtcatcga aacgtcttga attgcaaaaa ttacattaga cttcctgcaa aactagaatc
     1381 ctagttcatg attgataata ccagcactca aattcattcg tagtccgaag cgtttacgat
     1441 gatttcgata ggttgttgaa aacattttaa acgtttttac tttggcaaag atgttctcaa
     1501 ccttgcttct ctccttagat agcgcatggt tacaggcttt atcttcaact gttagcggct
     1561 tgagtttgct ggatttacgt gaagtttgtg cttgaggata tatcttcatg agcccttgat
     1621 aaccactgtc agccaagatt ttaccagctt gtccgatatt tctgcgactc attttgaaca
     1681 acttcatatc atgacaatag aaacaattct cccttgactt gtgacaatcg cttgagcctt
     1741 catagcgtga aatttctttt taccagaatg attcgctaat tgttttttta gggcgattga
     1801 tttttacttc cgtcgcatca atcattaccg tgtcctcaga gctgagagga gttcttgaaa
     1861 tcgtaacacc actttgaaca agagttactt caacccattg gctccgacgg attaagttgc
     1921 tttcgtggat accaaaatca gccgcaattt cttcataagt gcggtattct cgcacatatt
     1981 gaagagtggc cataagaagg tcttctaggc ttaatttagg ttttcgtcca ccttttgcgt
     2041 gtttaagttg ataagctgtt tttaatacag ctaacatctc ttcaaaagtg gtacgctgaa
     2101 caccaaccaa caagacgctt aaatcgtgca tcagttagtt gtttacttgc ttcatcattc
     2161 atagaactat agtaaaatga aataagaaca ggataaatcg atcaggacag tcaaatcgat
     2221 ttctaacaat gttttagaag tagagacgta ctattctagt ttcaatctac tatactatac
     2281 catattttgt ttcgcaggga atctattata aaagggtaag tatttcaaaa acacttaccc
     2341 ctttatactt tattaagctc tactttttat aatacttcaa tccccaaatg aaaggaagat
     2401 gttccgtttt ttagcaagaa aagttttcat ttctttgctc ctttcttttt agttaaagca
     2461 aaatagatca taactgcaat cacataggct atggtataaa atagctgata ccaaatactt
     2521 tccctaagcg gataaagaaa gatggacatg attagataca agatgaaaat gataagtatt
     2581 tttttcttca taaaatttcc tcctaaatgt gtgatttatc ttagttgaac aagaatcttt
     2641 acactgctag tatagcactc attttgacct tggattactc aaatcatgaa tggtcatcaa
     2701 aacatcttga acaataacaa gattttcatc tatacaaaaa tagttatact caacaacaat
     2761 caaactaaag aaccaaataa atatagctta acatactttt tttggatttc aaacaatact
     2821 gataaaatta ataataaatc tgtggtagta cttcccatcc ctactgcttt ccaattttat
     2881 aatattatta attaaataat atatgagaaa aaacgaacaa gactgtgtta ataatctatc
     2941 ttattcgttt tatttattag acttgttcag aaattatttg aagcagtatt agctggttag
     3001 aaagtgaatt tttaacgttc caaccagcca ttagtttcta gttttcgact cttgcaataa
     3061 gctaaaataa tcctgaacaa cacctgtata ttataattgc gaagattcaa caaaatagcg
     3121 aacaagtcga ttattcattt tttatataag tttacaataa ctttttaaca tggaactgga
     3181 ggtgcaggat tccacgaact ttttggaatg catactggag gaacatatgg agcaggtttt
     3241 atcatccctc cgatactctt caaaagcccc cagccgatac atccgatatc tccaataaaa
     3301 cagtcatctc caccactaat cgtctctaat tctacttcat tcaaagtaat ataatcaatt
     3361 ttttccattg ttataattca ttcctttcgt tttactcaag gcacaacaca gaatgaaaaa
     3421 gtgttgtact ctttatttta taatgtttat aataatagtg agaaaaccta tcactactac
     3481 aaatcacggg gaggtgaata agtgagtgat atagccaata tctcgcattt ttataggtgt
     3541 cattctttct atcaagcaca agaactgtga ctaggagcac gtaaacttat aacagaataa
     3601 gcgactcgtt tgcaaaagaa tagtttctat cactgcatag gtcattaagt tatataaatt
     3661 cttatacaat acaacactac tatatctata cacgaacaca gaaccactga tgaggatttt
     3721 tccggcattg attcgtaaaa tcatcaattg ctgtatcaac aatgctacct gcaacatagc
     3781 ctaaccctgc ccaaaacaaa gcatcgtcaa ctccaaatat tcctccctct atcctatcaa
     3841 gcatttcatt atccataaca gaaaattgtg acatcatttt tgtattcatg acaaatactc
     3901 cttttttatt tttaattttt gtcttgttgc aactttgaca agtttagtat atcattattt
     3961 tttaattttt ttcatccaaa tcttgaattg ccatcgaaac gtcttgaatt agctttttta
     4021 tttcaagcca cctctaaatg tttaaaaaaa ataatttcta atcacttttt taccattcag
     4081 gaagttttaa tgactattca agatttcata aaatatgaac ttagttttat ggcataatat
     4141 acctatccac tatatgaaag gaattgccaa tgacttctta taaacgtaca tttgttcctc
     4201 aaatagatgc gagagactgt ggtgtcgctg ccttagcctc gattgctaaa ttctatggtt
     4261 cagatttttc tctagctcac ttgagagaac ttgcaaagac caataaagaa gggacgactg
     4321 ctcttggcat tgtaaaagcc gctgatgaaa tgggctttga aacaaggcct gttcaagcag
     4381 ataaaactct ctttgacatg agtgatgtcc cctatccatt tatcgttcac gttaacaaag
     4441 aaggaaaact ccaacattac tatgttgtct atcaaacaaa gaaagactat ctggttattg
     4501 gtgatcctga cccttctgta aaaatcacta aaatgtcaaa agaacgcttt ttctctgaat
     4561 ggactggagt agctattttt ctagctccca aacccagcta tcaaccccat aaagataaaa
     4621 agaatggtct actaagcttc cttcctctga ttttcaaaca aaaatctctc attgcttaca
     4681 ttgttctctc aagcttattg gtcactatta tcaatatagg tggttcttat tatctccaag
     4741 gaatcttaga tgaatacatc ccaaatcaga tgaaatcaac tttaggaatc atctcagttg
     4801 gtctggttat cacctatatc ctccaacaag tcatgagctt ctccagagat tatctcctaa
     4861 ccgttctgag ccagagatta agcattgatg tgattttatc ctatattcgc catatttttg
     4921 aacttcccat gtctttcttt gcgacacgtc gtacaggaga aatcatttca cggttcacag
     4981 atgctaactc tattatagat gccttggctt ctaccattct ttctcttttc ctggatgttt
     5041 ctattctgat tcttgtaggg ggcgtcttac tggcacaaaa ccctaatctc ttccttcttt
     5101 ctcttatttc tattcctata tacatgttca tcatcttttc ttttatgaag cctttcgaaa
     5161 aaatgaacca tgatgtcatg caaagtaatt ctatggttag ctctgccatt atcgaagata
     5221 tcaacgggat tgaaactata aagtcgctca cgagtgaaga aaatcgctat caaaatatag
     5281 acagcgaatt tgtagattat ttggaaaaat cctttaagct cagtaaatat tctattttac
     5341 aaacaagttt aaagcaggga acaaaattag ttctgaatat ccttatccta tggtttggcg
     5401 ctcaattagt catgtcgagt aaaatttcta tcggtcagct gattaccttt aacacacttt
     5461 tttcttactt tacaactcct atggaaaata ttatcaacct ccaaaccaaa ctccaatctg
     5521 cgaaggttgc taataaccgt ttgaacgaag tctatctagt cgaatctgaa tttcaagttc
     5581 aagaaaaccc tgttcattca cattttttga tgggcgatat tgaatttgat gacctttctt
     5641 ataagtatgg ttttggacga gataccttaa cagatattaa tctcacgatt aaacaaggag
     5701 ataaggttag cctagttgga gttagtggtt ctggtaaaac aactttagcc aaaatgattg
     5761 tcaatttctt tgaaccctac aaaggacata tttccatcaa tcatcaggat attaaaaaca
     5821 ttgataaaaa agtcttgcgc cgtcatatta attacctacc acaacaagcc tatatcttta
     5881 atggctctat cttggaaaac ttaactttgg gcggtaatca tatgattagc caagaagata
     5941 ttctaagagc ttgtgaatta gctgaaattc gtcaagacat tgaaagaatg cctatgggct
     6001 atcaaactca gctctctgat ggagctggtc tatcaggagg acaaaagcaa cgaatagccc
     6061 tcgctcgtgc tcttttaact aaatctcctg ttttaatact agacgaagct actagcggtc
     6121 ttgatgtctt gactgagaaa aaggttatag ataatcttat gtctctaact gataaaacca
     6181 ttctctttgt agcccatcgt ctcagtatag ccgaacgaac caaccgtgtc attgttcttg
     6241 accaggggaa aatcattgaa gttggtagtc accaagagtt aatgcaggcg caaggtttct
     6301 accatcatct attcaataaa taaggagaat gtcatgaatc ctaatctttt tagaagcgtc
     6361 gagttttatc agagacgtta ccataactat gcgacagtgt taattatacc tctttcatta
     6421 ctatttactt tcatcttgat tttctccctt gttgccacaa aagaaattac tgttacttcc
     6481 caaggagaaa tcgcccctac aagtgtcatt gcctccattc agtcaaccag tgataatcct
     6541 atcctagcta atcatttagt ggcaaatcaa gtagttgaaa aaggggactt actcatcaaa
     6601 tactctgaaa caatggaaga aagtcagaaa actgccttag caactcaatt acaaagactt
     6661 gagaagcaaa aagaaggact tggaattttg aaacaaagct tagaaaaagc gactgatctt
     6721 ttttctggcg aggatgaatt tggctaccat aataccttta tgaattttac taaacaatcc
     6781 catgatattg aactgggtat cacaaagact aacaccgaag tttcaaatca agctaatctt
     6841 tccaatagca gttcatcagc tattgaacaa gaaattacaa aagttcaaca acaaattgga
     6901 gaatatcaag agttgagaga tgctatcata aataacagag cacgcttacc aactggcaat
     6961 ccgcaccagt caattttgaa tcgttatctt gtagcctcac aaggacaaac acaaggaact
     7021 gcagaggagc catttttatc tcaaattaat caaagtattg caggtcttga atcatctatc
     7081 gcaagcctca aaattcagca agctggtatc ggaagtgtag caacttatga taacagttta
     7141 gcaaccaaaa ttgaagtact ccgcactcag tttttacaga cagcctcaca gcaacaacta
     7201 actgtggaga atcaattaac agaattaaaa gtacaactag atcaagccac acagcgtttg
     7261 gaaaacaata ccttaacctc cccaagtaaa ggtatcgttc atctgaacag cgaatttgaa
     7321 ggtaaaaata gaattccaac tggtacagaa attgctcaaa tattccctgt catcacagat
     7381 acaagagaag tactaatcac ttactacgta tcttctgact atctacctct actagataaa
     7441 ggacaaactg taagattaaa actggagaag attggaaatc acggcaccac catcatcggc
     7501 caacttcaga caattgatca aactcctacc agaacagagc aaggaaatct ctttaaatta
     7561 accgctcttg caaaactatc taacgaggat agtaaactca tccaatatgg cttacaaggt
     7621 cgcgtcacta gtgtaactac aaagaaaaca tattttgatt atttcaaaga taaaatttta
     7681 acacattctg attaattttc agataacact ctataactat ttattatctt atcaaaaagg
     7741 agaatcataa catggataag aaacaaaacc taacttcatt tcaagaacta acaactaccg
     7801 aactcaacca aattacaggt ggaggattgt gggaagattt attatataac attaatagat
     7861 atgctcatta catcacataa gaacttcatc atccaataca actataaaaa aataagaccg
     7921 agaaacaagt actctcggtc ttatttttca tcattctgta tgtatcacag taagtacctg
     7981 acgaaagact tgattttgac aggtggtatt tagactggta ttaggatggc tttccacaat
     8041 cttcatgacg gtatagagac caactcctct ctcctcccct ttagaactgg ctccaaagga
     8101 gaagatttca gaaatatcga tgccctcttc tttgatggag ttttcgatga taaaggtctc
     8161 ctgtgctcca ttttttaaaa aggcgattga aacatgaggt tgactagctt ccacactggc
     8221 ttcaatagca ttgtcacaaa ggatagacac aatggttaga aaatcaagta gactcatccc
     8281 ctcgacctga atctcctcag gaacttcgac attaaagaca atgttcttat ctctggcttt
     8341 taaaaatttc cctgctagaa gacttttgag ggctttatca cgaatattta ccaatctgcc
     8401 caggtcatat ttattgttct gcaatttctg actggaatcc tttaagacgg agccatagac
     8461 ctcttttatc tgctccatat cctcctcttc aatgcccaga cgtaagctag tcaagaggtt
     8521 ggtataatca tggcgaaaac tccgtacttc cttgtaaagc tcctctatat gccgactata
     8581 gcgttccata tctctatagc gcagggcctg ctcttgttcc aatctctcat agagtttttc
     8641 cttcaaatag gtatccaatt tcttgataac ccccataaaa aagagtaggt aaaagactag
     8701 gatgagatgg cgaacagtct ttgattgaat actttgttca tattcaaaaa aagacagact
     8761 ttccatgact agatagtagc cacccattat ccagttaatc tgagtcaggg acttttgaaa
     8821 ggctttatcg agaatctcct ttctcaagct agtaaaatcg tagtccaacc atttcaaaaa
     8881 agctagagaa atgaagaaat tgaaaattat tatacataac ccagtaaatg agtagccatc
     8941 atatacttgc ccttgtccca aaaatggaag cacaaaatag gagactcctc tataaaagag
     9001 attcaccaat atcattggaa agagaccata aaagaaaagg agttttttag gaagccctct
     9061 caataataag aaagataagc ctatgccgta caagggttcc ataaaataag ataggtaaac
     9121 atttcctact atatagctaa tcatcacaaa aacaaaggcc aacagtatct taaaaagaaa
     9181 ggccttaaaa atcctctcga aagcaagatc aattccatcc accttaaaga agatgacaat
     9241 ttctagtcca ttagtaacaa gtgtatacaa caatatccaa gcaatgttca taaattctcc
     9301 tagctcagtg taatttattg atggcctcag acacttccct gaccttataa cgggcgatta
     9361 gacaacttcc accattggga aagaaaagca gtttttcttt cttatccaaa tgcaccacat
     9421 ttgcaggatt gatgagaaaa gagcggtggc actgcaagag acggggctcc tgcttgaaaa
     9481 cctcctctaa actcgctgta aattccagcc tgtctgtctt ggtatagaga ataacacgat
     9541 gggctctggg cgacgtttcg agatagtaaa cctctttaaa aggatactga aattgggcaa
     9601 attttgattt aaagtaaaag caatcttccg ccagactttt actatcttga ctattggcat
     9661 agaggagggc tgtctcgatc cgagattcaa actcctctgc tgacaaggcc ttatcaatgt
     9721 agtccaaagc agacacttgg tagcgaaaag acaggggcat aaactccgag tgagtcgtca
     9781 caaagacaat cagggcataa ggatcccgat cccgaatctt tctagccact tccagtccct
     9841 tcatctcttc atttcgaatc tcaatatcca aaaagaatag ctgatgggcc cccttctcat
     9901 gcacttcagc cagcagttgg tccggcttgc caaatacctc aaaagagcta ggaatgatat
     9961 gatgtgcttt caaaagtttc tcaatcgtcg tttcaattct agtctgttgg gaaaaatcat
    10021 cctctaaaac aaatattctc atcttcttac tctccctctt tcaaccattt ttgacgaatt
    10081 tctctatagc gacgtctggc atacttgaca gcgtattgac cttcttctcc gagaaaaagg
    10141 attctttctt gaaaatcaat cgattttaat ttatcaagat taaccaaaca atttcgatga
    10201 catctcatca aggtttcccc acattggttc tcaagattac ttaaattttg aagcatatta
    10261 aaactagctt cttctgtaac aatctgtacg gtatgggctt tagttggatg tgtttggata
    10321 tagtagatat cgtcgatgtt tactttatag actgttttct gcgtctgaat aatcatatat
    10381 ttcattggat tctcctccta agaaaagaat aacagaaaag gtctagccca agcacaccca
    10441 atcctaaaca gtctttgaaa actcctaaaa ggattatttt tctacaacat catgatgagc
    10501 ttacaactat tttatcataa aattcactca gcgcaattca agacatttca atgacaatta
    10561 aagatttgaa taaaaaaaaa gaattaaaaa tgataaacta acagtatcaa aacacagttg
    10621 ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat acagacccca
    10681 tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca tgcaaacagg
    10741 caagcgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt tttctagcca
    10801 aggctgaaac agtggataat cgattcttga ttgaactaga aaaattttac cagacttcca
    10861 gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg
    10921 cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat gaaaatcaaa
    10981 gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaaggtga cgctgacgtg
    11041 gtttgaagag attttcgaag agtataagtc tctacggacc aaccattatc ctgtagtaac
    11101 aaaaaagaag ctgagacaga ctgaactcaa cttctttttt agtgtcgtat cagcaatgtt
    11161 agtcctgcat ctgacgtttt acctgataag gcaaatacaa gactagtaaa accaaaccag
    11221 ctcctagtaa ggctagaagg gccagttttt cttggaaggt aatcaaccca acaactaatt
    11281 ccacaagtaa aacaaaactg gtcaatcctc gaactacctc ctgtaagcct ttttcctttt
    11341 gccccttgtc cagcggaaag agttgggtca aatactggta gtcaaaggca tgatagaggg
    11401 ccagcaactg gaagagcaag aggtagttaa agagaactac cactgctgtc gcaatccaag
    11461 cttgctcgat aaaaacctgc gccagtacgg aaagtaatag aagacgaaga ctgagagcaa
    11521 agaggtcgcc atttcgcaga taagaacgca gatagagatt ttgccaaatc ttcccaggca
    11581 ccttctgaac agcctttaag ataaagtcca gataggcacg acgcttaacg ctgtttgaaa
    11641 ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa gacttgctta cgcttgcttt
    11701 cttgagaaat aacatagtcc cagtccagtc cagtttcagt gaaaaatttg ctggcctttt
    11761 gacgaaagtg gaaatatttt cctaccccca ataaaagcac atagagcaga aaaactggca
    11821 agccataacc cattgctaaa aataaaggcg caaataacag caagaaaagg gtctgtacaa
    11881 agagccaaaa gactagggaa atgccagttt gacgcttgag atggagcttg atttcctctt
    11941 ctccaactaa gagaaagagc ttgtctggag cctccatata ggtggcaatt cctccccaaa
    12001 gtaaaagtaa aacagacgta attcctacaa acaaaaggat aggccaatga ttttcaggaa
    12061 aatgttgtaa gagttgactg tactggtagg ctagaaagcc caacaggaca agcaggaaca
    12121 agacaaagtg gtcattgagc acatagcgca gataaccaag acactcctta cgaaaggcct
    12181 gctttctctt taaaaacaag tctttcatag atcctcctct ttggtcagag ccaagtaaat
    12241 atcattcaaa ctagcctcag gcatatcaaa ggcttcacgt agttgcagga gattgccttt
    12301 ggcacgcacc tctcccttgt gaagaatgac aaaggcatca cacatcttct ccgccgaatc
    12361 cagcacgtgg gtactcatga gaatagactt gcccttttgc ttctccactt ccaaaagctg
    12421 aatcaaatca gaaatagcca gcggatcaag accaaggaaa ggctcatcca cgatgaaaag
    12481 acttggatcc accacaaaag cacagataat catgaccttc tgcttcatcc cttttgaaaa
    12541 atgaacaggg aaccagtcta atttctgttc caaacggaac atttttaaca agggctctac
    12601 tcgttcgaaa gtcacttttt gctcaatacc gtaagccata gcaaccgttt cgatatgctc
    12661 tctgagagtc aattcctcat acagactagg cgtctcagga atgtagccaa tttgcttgcg
    12721 gtagctcgtt gcatcttctt gcagggtcaa gccattaata ttgatggaac cactataagg
    12781 tgtcaacaga ccgataatct cattaatggt cgtcgattta ccagcaccat tgagaccaat
    12841 caaaccaacc agctgcccac tttcaacggt aaaggacaca tctttcaaaa caggaacatg
    12901 aacatagcca cctgtcaggt ttttaatttc taacatattt tctccaaatc tggtataatg
    12961 tagctatatt atatcaaaat tcagtacagt agaggtagat tttatgtcag attgcatttt
    13021 ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta tatgaagatg agcaggtcct
    13081 tgcctttctt gatatctctc aagtaacact aggacacacc ttggtcgtgc caaaagaaca
    13141 ctatcgcaat cttttggaga tggatgctac gagcgccagc caactctttg cccaagtacc
    13201 aaaagtagct caaaaagtca tgaaagtcac taaggctgct ggtatgaata tcatttccaa
    13261 ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac gttcaccttg tgcctcgcta
    13321 cagtgctgac gatgacctca agattgattt tatcgcccac gaaccagact ttgacaaact
    13381 tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt gctatgaaat tatccaacct
    13441 actgctattt gcaggagctg cagccggaag ttatctggtt acaaaaaatc gccaaaccat
    13501 cacagatgaa gtcttgaata ccactgaccg cgttcaagct atcaaagacg atgtggatat
    13561 tatccaaaat agcctgcaaa tcatcgacca gcaaaaagaa cttatcaagg aataccaaga
    13621 agacttgact tacaagttta aggtcttgga aaaggatatc cagactagac tagctgtgat
    13681 aaaagaaatg cagggaactg aagataagta aaaagagccc cccggctctt ttttaaaacg
    13741 taaacaagtt atagtgaatt gaatttagaa tagcacatca tagtttctaa agcattgtta
    13801 gagattactt taaaatcctc ttatcaattt gttcatattc tatttcaatc tactatatat
    13861 atcccatcaa ctatgcatca taatttaggt aactcatact caataaaaat caaaaagcaa
    13921 actagaaagc taggcacaga ctgctcaaaa caccgtttta aggttgtgga tagaactgac
    13981 gaagtcagct caaaacactg ttttgaggtt gtagatacga ctcacatata tacggcaagg
    14041 caacgctgac gtggtttgaa gagattttcg aagagtatta ttctccatca aaggcatctt
    14101 ttatatggtc aaagaagcct tttttctttg gatttacttt caagtcacca gcagccgcga
    14161 attctttcaa ggctactttt tggcggtcgt tcaagcctgt cggtgttacg acattaacag
    14221 taacgtattg gtcaccaact gcaccgccac gaaggctcgg tgccccctta ctacgtaggc
    14281 ggaatttctt accagtctga gttccctctg gaataaccaa ttcaacatca ccgtgaacag
    14341 ttggaatatc tactgtatca ccaagagccg cttggacaaa gttgaggttg agattgtaga
    14401 agatagtcgt tccttcacgt tcaaacttgt cgctagcttc tacagaaact actacataca
    14461 agtccccata aggtccaccg ttaaagcctg cttcgccttg accagcgagg cgaatttgtt
    14521 gacctgtttc cacaccagca gggattttca catgtacgct atgagcttgt ttctcatgac
    14581 ctgttccatg acaggttgta catggatatt tgatttcttt tcctcgaccg tgacagacat
    14641 cacaggttac ttggcgacgc atcataccaa gaggagtctg cgtatcgacg ttaatgacac
    14701 cagcgccatg acagcgtcca caagtgactg gacttgtccc tggcttagca ccagagccat
    14761 tacatgtacg acagccagct tcacgatgat acttaacttc cttctcagtt ccgaagatag
    14821 cttcttcaaa ggtcaaattg acacgatact ggagatcatc tccttggcga ggagcgtttg
    14881 gattgcgcga agaaccgcct ccgccgaaga aacttgagaa aatatcctca aaaccaccga
    14941 agccacctgc cccattgaaa ccaccgaaac caccagctcc accaaaacca ccattggcgc
    15001 ctgcagcacc atactggtca taggcagcac gtttttggtc gtcactcaaa gtctcatagg
    15061 cttcttgaac ttccttgtac ttgtcctcag caccaggctc cttgttgata tctgggtgat
    15121 attttttgga aagcttacga taagcctttt tgatttcgtc tgccgaagcg tttttggata
    15181 cccccagacg atcataaaat tcagtattgt tcatacaaga taccaagacc gtaaaattcg
    15241 actgaaaaat aggaaatctg acgctggagc gacgctccta gacagatttc tcttttttcc
    15301 gagaatttag gtcgggttca attcctttct tttatattga tgagataata ctccagccac
    15361 tcggccctat ccctttttat cgaaaaacag aggctgggtt gcaacctctg caaataagta
    15421 aattaacttt attttgattt taaatatcct ataagtacag aa
//
LOCUS       DAVKWS010000044        15176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKWS010000044 DAVKWS010000000
VERSION     DAVKWS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.044"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            73..1743
                     /locus_tag="VW852_001537"
     CDS             73..1743
                     /locus_tag="VW852_001537"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV0272658.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCALIDVPVELASVWADGVEGGVELAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQVVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            complement(2234..3400)
                     /locus_tag="VW852_001538"
     CDS             complement(2234..3400)
                     /locus_tag="VW852_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0272659.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(3413..3820)
                     /locus_tag="VW852_001539"
     CDS             complement(3413..3820)
                     /locus_tag="VW852_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV0272660.1"
                     /translation="MMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYY
                     NDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKLKEAFDQK
                     QIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            complement(3817..4152)
                     /locus_tag="VW852_001540"
     CDS             complement(3817..4152)
                     /locus_tag="VW852_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV0272661.1"
                     /translation="MHIEHLSHWSGHPNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGRVQFFTLSSVDSESWLATWKNAHDQAEMHRAYERYVIEEAILLS
                     STRQVSWMA"
     gene            complement(4174..4987)
                     /locus_tag="VW852_001541"
                     /pseudo
     CDS             complement(4174..4987)
                     /locus_tag="VW852_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(5122..6336)
                     /gene="thiI"
                     /locus_tag="VW852_001542"
     CDS             complement(5122..6336)
                     /gene="thiI"
                     /locus_tag="VW852_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV0272662.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(6345..7487)
                     /locus_tag="VW852_001543"
     CDS             complement(6345..7487)
                     /locus_tag="VW852_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0272663.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHSLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDGVQALTKIPTEKYLTERVDFATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NHPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            7625..8053
                     /locus_tag="VW852_001544"
     CDS             7625..8053
                     /locus_tag="VW852_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272664.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            complement(8098..10323)
                     /locus_tag="VW852_001545"
     CDS             complement(8098..10323)
                     /locus_tag="VW852_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV0272665.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(10487..12439)
                     /locus_tag="VW852_001546"
     CDS             complement(10487..12439)
                     /locus_tag="VW852_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0272666.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(12436..13347)
                     /gene="pfkB"
                     /locus_tag="VW852_001547"
     CDS             complement(12436..13347)
                     /gene="pfkB"
                     /locus_tag="VW852_001547"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV0272667.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(13344..14084)
                     /locus_tag="VW852_001548"
     CDS             complement(13344..14084)
                     /locus_tag="VW852_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0272668.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            14247..14334
                     /locus_tag="VW852_001549"
     tRNA            14247..14334
                     /locus_tag="VW852_001549"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14281..14283,aa:Ser,seq:gga)
     gene            complement(14504..14851)
                     /gene="ssrA"
                     /locus_tag="VW852_001550"
     tmRNA           complement(14504..14851)
                     /gene="ssrA"
                     /locus_tag="VW852_001550"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         4426 a         3365 c         2973 g         4412 t
ORIGIN      
        1 gagttttagg atgttaaaaa tcttgttttg tgatataatg aattatcata taagaggtta
       61 gaggaatttt gaatgaaaac agatattgaa atcgcacaga gtattgagtt gaagccaatt
      121 gttgatgttg tagagaaact tggtatttct tacgacgatt tggagttgta cggaaagtac
      181 aaggctaaac tcagctttga taaaattcgt gcagttgaga gcaatccagt cggtaaattg
      241 atcttggtta ctgccatcaa cccaacacct gcaggtgaag gaaagtcgac gcttaccatt
      301 ggtcttgcgg atgccttgaa caagattggc aaaaaaacca tgattgctat ccgcgaaccg
      361 tctcttggtc cagtcatggg gatcaagggt ggtgctgctg gtggtgggta tgcacaagtt
      421 ctgccaatgg aagacatcaa cctccacttt actggagata tgcatgctat tacaactgcc
      481 aacaatgccc tttctgcctt gattgacaac cacttgcacc aagggaatga gctgggaatt
      541 gatcaacgtc gtatcctctg gaaacgcgtt gtggacttga acgaccgtgc gctccgccat
      601 gtgactgttg gtcttggtgg tcctctaaac ggtattccac gtgaggatgg ttttgatatt
      661 acagttgctt cagaaatcat ggcaattctt tgcttggcaa cggacatcga ggacttgaaa
      721 cgtcgtttgg cgaatatcgt tattggttat cgctatgacc gtacgcctgt ttctgtaggt
      781 gatttgcagg ttgagggtgc cttggctttg attttgaagg atgctattaa gccaaacttg
      841 gttcagacaa tttacggtac acctgccttt gtacacggtg gtccatttgc caatatcgct
      901 catggctgta actctgtttt ggcgacgaca acagcccttc acttggctga ttacactgtt
      961 actgaagctg gttttggtgc ggaccttggt gctgagaaat tccttgatat caagacacca
     1021 aacttgccaa catctccaga tgcagttgtt attgtcgcaa cccttcgtgc ccttaagatg
     1081 aatggtggtg tggctaaaga tgctctgact gaagaaaatg tagaggcagt tcgtgcaggt
     1141 tttgctaact tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc agcagttgta
     1201 gctattaacg aatttgtatc tgatacagaa gctgaaattg cagccttaaa agaactctgt
     1261 gccttaatcg atgtaccagt tgaattggct agtgtctggg ctgatggagt agaaggtgga
     1321 gtagaacttg ccgaaacagt tgttaagaca attgctgaaa atccagctaa ctataaacgt
     1381 ttgtatgata atgacctttc tgtccaagaa aagattgaaa aaattgtcac tgaaatctat
     1441 cgtggtagca aagtgaactt tgagaagaaa gctcaaacac aaattgctca agtcgttcaa
     1501 aacggttggg acaaattgcc aatctgtatg gctaaaactc aatacagttt ctcagacaat
     1561 ccaaatgcgc ttggagcacc agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa
     1621 ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc aggtcttcca
     1681 aaacgtccag cagctctcaa catggatgtt gaaagcgatg gaactgtact aggcttgttc
     1741 tagtggattg aaattgactt taagtgagtt tggaaataat atccaagctc attttttctc
     1801 agactgcaag cagttcatca ataagattta tactcttcga aaatctcttc aaactacgtc
     1861 agcttcacct tgccgtaggt atatgatact gactctgtca gttctatcca caacctcaaa
     1921 acagtgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
     1981 ttaatagact ggtttggtat cctgaaaact atatcgtata ggtgtcgtca tggatccttt
     2041 tagctgattt aaggatacga aaacacaata ctatgactaa attgaaatgt taaaattcaa
     2101 tttctagcca cctaatcctg cttcaaatag ttatcgaaca agttttatga tagctacctt
     2161 gttaagatgc tcttattttt taccagacaa aacaaaagga ttaagggagt gaatttcctt
     2221 aatccgatag ttcttattct tgacgttgtc cgaaatcagc aatcatctga tccatttctt
     2281 gtcgactcgg agttgtcagc atatacaggt aatctccttg aagttccgcg aaggcagctg
     2341 gcatgacttt tttaaccttg aactgctggc tatatttggc aagcaaatcc tcttctgaat
     2401 aaacatagtg agcatttgca cggcgagaag tagccaaaca atactgagtt tcaaagtctg
     2461 acgccgggaa ctcctctcct gcgacaatag ctgcatagcc acgataaagg tccaaggaat
     2521 gagcaaagtt ataaacatca atggtaaaac cacctgcagg gcggttattg tactcgatgg
     2581 taatataatc gtccccctca cggaagaact caatatggaa aaaccgttct ttcataccaa
     2641 attctttgac aattgcttcc ccatacttgc gcagtttagg atccatatcc ttgagcacat
     2701 aataagaatt gtccatctta taaatcatga ggtcaagcgg tgtataggcg tagtcaaagg
     2761 ttgttgagaa gacaatcttt ccatccttgt ccacgagccc gtcaaaggta cagatttcgc
     2821 tggaagtgac aaatttttca aagaaataaa gggttgaatg gtcccattct tgcttgaagt
     2881 gattgatatc gtcttctgtc tcaagtttaa aggttgcggc tgctcccact ccattatcag
     2941 gtttggcaat cattggaaga ccgatttctt tcactgcttg atcaacatct gcttccgtct
     3001 tgataacagc tccaggtacc acaggaacac ctgctttttt gaaaagtttc ttcatttcag
     3061 acttatattt cgtctttttg agatcctctg gtttggcacc aaaaacattg aattgttctc
     3121 tgagtgttgc gtctagctca agccagtatt cattgtgaga ctcgatgcgg tcaattggac
     3181 catgtttata aaagagaaaa gcaactgcac gtttgacttc atctatgttc tcaagattat
     3241 caacacgaaa atactcggtc aagctattgc gcaagggctc atccaattgc tcgtaagact
     3301 cttgaccaat tcccaagact gtgatgcctt tattagctag ttcgatggta aactgttgaa
     3361 agttttgtgg atagtaggga gaaataacaa ggtaattcat aggtaactcc ttttataaat
     3421 agagattacc gaggaaataa ggcatttgtt tacgccacca ttcccagtcg tgggcgacat
     3481 catgtcccca ttcagcaaac caggctggaa tttgtttctg gtcaaaggct tctttgagct
     3541 tgtaaaagga tggtaaacca tcttgttccc aggctccaag ccccgtacac agcacaatct
     3601 ctgcctgacg gtaacggtca ataaaccagc catcgttttg gttccaaata taatctactg
     3661 gcgagttttg gtaaatagca tcatcgttgt agtaatcacc gacaaagaaa cgtgcgtcgt
     3721 aaacaccact gagagcaatc actttggtaa agacatctgg atgctggagg aagaaattga
     3781 gtgcatgata ggctcccata gagcaacctg tcgtcatcat gccatccaac taacctgtct
     3841 tgtgcttgat aaaagaatgg cctcctcaat cacataacgt tcgtaggcac ggtgcatttc
     3901 cgcttggtca tgagcatttt tccaagtagc caaccagctc tcactatcca cactagatag
     3961 ggtaaagaac tggacacggc cttcctcgat aaaggaagca caggcatcaa tcatgccaaa
     4021 atcatagtat tcgttgtgac taccacctga tgaagcaaag accacaactg gaatcccacc
     4081 atgtccataa cggttaaggt acatttcacg gttaggatga ccactccagt ggctaagatg
     4141 ttcaatatgc atttcgtttt ttcctttctt aacttaccat ttttctgcaa aaaatctcag
     4201 acaatctggt agattttctg accaagggat ttcactatgg atggcaccag actgaacttt
     4261 tagcacaaga ttatccagat gtactccccc tgctatcaaa tcatggtaat agcaaagcga
     4321 cgagtcgata taggcttgtt tgatattgcc atccatcaag gtcttgtctg tatcatctgc
     4381 ttcttctgtt cctacataga tgaagatgcg ttggtcaggc gatagtttct ggcactcgat
     4441 atagcggttg gtggagccag tttgcagatg aaaaaacgcc caagcaacca attttgtctt
     4501 ggtattccaa accgataaac tgggtaatat tgcctcctag tgaggaacca atcatagccg
     4561 tatgctggca gtctgctttt gtacgatagg tctcatcgat aaaaggcttg accacctcca
     4621 tgacaaactc agcatactcc acacccttac caccaaactg ctgccctggg ataggagatt
     4681 cttggaactt ccaagccgca tactcattca tccgccccat accatcattg tcaatagcaa
     4741 cgacaatcat gcgactgata tccggatttc gtttgatagc tgggataatc ttccatgaat
     4801 gtccaatgaa agactctttg ctattaaaaa cattttgccc gtcatgaaag tatacaacag
     4861 gataggaacg gtctgtatct ttctcataat ctttaggaag aagaatacgt acacggcgct
     4921 ccttacctgt ataaggaacc ttgagtttgt gttctttcat ttttagataa aagtaggatt
     4981 gattcattgt cagaaaactc tctatattca aaattttata tcattatatc ataaaaataa
     5041 aaaaagttag ttttttacct gtttttagag aaaaaactaa ctttttttac tgattttcgc
     5101 tattcttttg gattttctga attagagcag attgtcaatc aagtcatcaa cttcatcttt
     5161 ttcggcttga ggtgtgattt cagtaatcat gattccagcc actgctcgct caaccaagcc
     5221 ttcaacatcc atacgcgctt cgtactgctc cgcattctta attttaggat ttgtttttgg
     5281 acgatctggt gcaaaaatgg tacaacagtc ttcaaacggt tggattgaaa tgtcaaaggt
     5341 atcgatttcc tgggcgatgt caatgatttc caacttgtcc atggtaacca caggacgaat
     5401 gatgggagtg ttggtaacag cattgatagc cttcatactt tcaagggttt ggctggctac
     5461 ttgacctaga ctttccccat tgatgataac caaaccattt cgtacctcac gaatacggtc
     5521 agtaatccgc atcataaagc gacgagttag agtcatcaaa taagcttctg gcgctttggc
     5581 tttgatttcc tcttgaatct ctgtgaaagg cacctcgata aactggatat ttccgccaaa
     5641 cttggtcaat ttacgggtca agtcctgcgc tttcttgagg gcaccaggac tagtatatgg
     5701 tggactagca aagtgaactg cctcgatatc caccccacgc ttaagagcaa gataacctgc
     5761 tacaggtgag tcaatccctc ctgacaacat gagcatccct ttacctgaag ttccaactgg
     5821 caaaccacca gccccacgaa tggtttcata agaaagatag gctgcttctt cacgaatctc
     5881 cacctgaaga ttgatgtcag gacttttcat ttgaacttgc acatttggaa tggcttcgaa
     5941 tacagcccct ccaagtgttt ggttgagttc acgactatca agttcaaagt tgtggtcgct
     6001 acgcttgcta gaaatcttaa aggtcatacc ttccttgtag atgtcccgca taatctcttg
     6061 gacagaagac ttcaaaactt ctacagattt ttcaacctta taaacaggag aaaagttttg
     6121 aattccaaaa acttgtttga gagattctgc aactgctgtg taatcagctc cattgaggta
     6181 agcgtgggca cggtcgcgat ctgctgttac cttaacttgg gtatagatag acaaaacgtc
     6241 cgaaatatta ttacgaagtt tattgatgaa acgcatacgg tttttaccct tggttgacaa
     6301 ctctccgtag cgaatcataa tttctgaata ctgcatgaat gctcctatct tacttttcta
     6361 gtttgattgt aaatcaattt taacttggtc aaaaactgct cgacctgact catatcattt
     6421 tccaagtcta ggctaagacg cacagctgac ttggccttat ctttgtccac tcccatggca
     6481 atcaaggtac cggctggttt tcctgcctta gatgaacaag ctgaggttgt tgagatgaaa
     6541 atatcatagt cttcaaaggc gtgaacgatg acttcacctc gaacaccttt gattccaaaa
     6601 gtcagaatat gaggtgcaaa gttttcctca tctgaaaaga caaaaatatc cggatggttc
     6661 agaagagctt ggcgaatcac tgccttcatc tgcccagtct tgctcctaaa gatatctagc
     6721 ttttccatag acaaacggag ggccttggct gtcgctgcaa tccctgccac attttcagtt
     6781 gtcgaacgat aatctcgctc ctggccacca cctgtaagaa gaggtgtaat cttcttgcca
     6841 gatttgatat agacaaagcc aacacctcga accccgtgga acttgtgact agagaaagtc
     6901 gcaaaatcca ctcgttctgt cagatacttt tcagtcggaa tcttggtaag cgcctgaacc
     6961 ccatcaacgt ggaaggaaat agtcggcttg tctgccaaga attctgaaat agcctcaata
     7021 ggttggatag agccgatttc attgttcaca gccatgatag aaacgaggat tgtatcaggc
     7081 cgtatcaaac ctgctaacgc ctcaacatcg accaagcctt tcttatcaac tggagcaaaa
     7141 tccacttcaa atccttgact tttcaaccag agggctgact ctttgactgc tggatgttca
     7201 atggctgaaa caatgatgtg cttgccaaac tgagcttttt caaaggccac acccttgata
     7261 acccagttat ccccttctgt tccaccagag gtaaagaaga tttcatcgct tttcttaccg
     7321 attaaatctg caatctgttg gcgagaagca tctaagattc gtgttgcttg gtctcccaaa
     7381 ctatggagac tagatgggtt tcctaaaatt tttgaagcga cctgcatata ggtttcaagg
     7441 gcttcaggat aaggcttggt cgtcgctgaa ttatcaaagt aaatcatgtt tctcacgctt
     7501 tctaaaatca cttcctctat tgtatcatga aacggagctt gcgacaagaa agggcaattt
     7561 ctcttttttt aagatttttt taaagaaaca gagtataata aatcataatc aaaggagaga
     7621 atgtatgtct aactatcgta gaacttcaaa accaaaaaca gaacacatca aaaaaggctt
     7681 tacagtcttt caaaaaaccg ttgctacaat cgctagtatc cttggcttga ttaccgcaag
     7741 tatcacgatt atgaacgcct tggataataa caaaaatatt aaaaaagaac ctacgacaag
     7801 ccagacgaca accattgtca aagaaatcca aaaggaatcc cctaaggaaa acaccagtcc
     7861 aactaaagag actaacacta gtcaagaaaa aacacagcaa gaggaaacac caaaatctag
     7921 cgtcaaggaa gagaaaaaag aagatcagaa aacagcaact caggactctt ctactcctgc
     7981 ttcaagtaaa cctgctactg aaaacgaaaa acagtccaat gcgccaactt cagaaaataa
     8041 aagcaatcaa tagccactaa aatagcttcc ctccaaactc ggaaagaagc tattttttta
     8101 ttgttgtaac acttttcgag gtttggtacc ttcagctgga ccgatgacac ctgctatctc
     8161 cagttcttcc atgagacggg tcgcacggtt aaatccaact gataaacgac gctgaatcat
     8221 agacgcactg gctttctgtg tttcgataac caaagactta gcttcttcaa aaagtggatc
     8281 accaccagca tctccatccg aaaattctcc ttcattttca gaaacctcac ctggatcaaa
     8341 actctcatcg tagtctgcat ctgcctgagc cttgatgaag ttcacaatgc gctcaacatc
     8401 gtcatccgag ataaaggagc cttggagacg aactggatga ttttcatcaa tcggtttaaa
     8461 gagcatgtct cctcgaccaa gaagtttttc tgctccattt tcatccaaaa tcgtacggga
     8521 gtctgttcct gatgaaaccg caaatgctac acgagatgga acattggcct taatcaaacc
     8581 agagatgaca tcaacagatg gacgctgagt tgcaagaatc atgtggatac ctgcagcacg
     8641 cgccttctgc ccaagacgga tgatagcatc ttccacttcc ttgctggcca ccatcatgag
     8701 gtcagccaac tcatccacaa tcacgacaat gaatggtagc ggaatttgct tgtactcaga
     8761 ctgggaattg aactcttcta ccttggcatt aaaacctgca atattccgaa ctcccacctt
     8821 ggcaaagagt tcataacggt tttccatttc atccacaacc ttttgcagag ccttgctggc
     8881 tttacgtgga ttggtcacga ctggaatcaa gaggtgggga atatcattgt aaacagataa
     8941 ctcaaccatc ttgggatcga ccatcataaa tttaacttga tctggtctcg ccttcatgag
     9001 aatgctagca ataatgccgt taactgctac tgacttccct gaacccgttg aacctgcaac
     9061 tagcaagtgg ggcattttag aaaggtcaaa agctcttgcg gttccattaa cagccttccc
     9121 taaaggaatt tccaagaaat tttctgcttt cgtttgcgat tgttcccata gttctcggaa
     9181 agatacagtg gcaatatcgg agttgggcac ttcaattccg attagggatt tcccagggat
     9241 tggtgcttca atccggacat ctttggcagc caaggctaga gcgaggtcat ctgatagatt
     9301 ggaaatgcgg ttgaccctta caccaacagc cggcttgact tcatacttgg tcactgatgg
     9361 cccaatttcg gcccgttcaa ctgttacctt aataccaaag ctagcaaagg ttgcttctaa
     9421 gattttgata ttttctctga caattttctt ctctttagac tgatcttttg gtttatctgg
     9481 tgcaaagagt tgtaagcttg gaagtttgta ttcaagggct tctttggctg aaaaatcgac
     9541 ctgaacatct tcgtcatctg agtcatcttc ctgttcaggg aatttaagtt cagcttgagg
     9601 caggatgatt tctggttcca cccacttttc ttctggaata ggtggaagat tttgaacagc
     9661 ttcctctgtc agaatttcac ccgtttccat atcaacagga ggcaaatcga gtaaggcttt
     9721 ttcagtctct tcttgttcta atctagcctc tttctcagcc ttttggcgag ctttttcttc
     9781 ttgtttgaca aagcgttcct cttttcgacg ctcgtgccct tcccaccatt tggcaaagcc
     9841 tctactgaaa aattcagcaa tatcgtaaac agaccaaggg ctgactagga gagaacccac
     9901 taaaatcaag atagaaccaa taaagtaagt tccgatattt gaaaagagaa aggctgttgg
     9961 aatataaaga gcgaccccga tcaagccccc tccagcaaag ctagtcgttc gaaaaccagt
    10021 cagatctgtc acaacctgag ccatggtccc ttttagaacg gacttgtcca aaccatattt
    10081 ccaaaccaag taggcctcaa aaatcaagag taagccagca aatatggtga aaaaaccaga
    10141 taagagtcct tcctgttttc gtatccactt gaaaaagaag agatagatta ataggccgaa
    10201 tatcgccaga taagctaggc tacccactag caagcgaatt aaattataaa gggttatacc
    10261 tgcagcccct aatttgaagg ctgcgaaaat caataaaatc gcaattccta acgaaatcaa
    10321 cattcgttga atcgcttctt ttctttccag ttctgcttta gacggtctcc gtcttgttgt
    10381 acttgtattc ttgtttgcca ttcttctatt ataccatatt tcacaggcat ttcgggaaga
    10441 gaaaaagact gcaccaaacg gtccaatctt ttcatttttc tattttttat gcttgtggtt
    10501 tgcgtaggta accataaacc acaccactta cgattgctcc taccaagaca gaaacgaggt
    10561 aaaggagagc atttgaagta agggcgataa cgaagattcc tccgtgtggc gccatgagtt
    10621 tgataccagt aagaccaacg agtccacctg ctactgctga accaaggatg aagcttggaa
    10681 tcgcacgagc tgggtcagcg gcaccaaatg gaatcgctcc ctcagtgata aatgacaagc
    10741 ccatgatgat gtttgtcaaa ccagagttac gttcttcctt agtaaattta tctttgaaaa
    10801 gaagagttgc gacaaagatt gcaagtggtg gcaccattcc tccagccata actgctgcca
    10861 tggctacaga accacctgaa gaaacagttg ctgcaagcgt acctgtacca aagacataag
    10921 ctgctttatt aactggtcca cccatgtcaa cagccatcat tccaccaagg acgataccaa
    10981 gaaggacagc tgaacctcct ccaagaccac ctaggaagtc attcatagca gtgttgattg
    11041 cagccattgg gatattcaca gctagcataa caaatcctgt caagattgtt ccaagaagtg
    11101 gcaatagaag gattgattta gcaccttcga gtgaacgagg aactttaacg tatttcttga
    11161 tggcaagaac caaggcacct gcgataaatc caccaacaag ggcacctagg aaaccagatg
    11221 agacacctgc aagagttgaa gttgcttcac cacctgcggc ataaggaatt ttaccaaagg
    11281 caaaaccttc tttggcaata gcaccagcca cgaaacctgc taccaaacct ggtttttcag
    11341 caatagagta ggcaacataa cccgcaaaga ctggaagcat caaaccaaag gcagctccac
    11401 caattttcat gaacatagaa gctaactcat ggtaagaacc aagattgcca aggttttcat
    11461 ttggaacact caaagcaccg tcaatcaaga aggcaagggc aatcatgata ccaccaccga
    11521 taacgaatgg taacatttga gatacaccac tcatcaagtg tttgtagaag gcaccaccaa
    11581 ggctttgttt ttcgttagag gctgttgcag cttttgcacc attagcggca cggtagactt
    11641 cagtatctcc tgaaagagcc aagttaatta gctcttctgt cttacggata ccgtcagcaa
    11701 ctggacgatt gatcaatggt tttccatcaa atcgatccat ttcaacggcc ttgtctgctg
    11761 caatgataat agctttagcc ttacggatat cttctgcagt tagttgattt ccaacaccgc
    11821 tagcaccgtt ggtttcgacc ttgataccaa cccccatttc agcagctact ttttgaaggg
    11881 cttcttgggc catgtaagtg tgggcaatac ctgttgtaca agctgtaaca gctacgataa
    11941 agtcaccaga gtcattagca ggtgcttgaa caagttcctc agttttttct gaagcttggt
    12001 caaaaagttc gataacttgg tctgcagatg ttgcttgacg aagtttgtct gcaaaaccgt
    12061 ctttcatcaa gtattgagac aattctgcca aggctgccaa gtgagtatca ttggcacctt
    12121 ctggagctgc aatcatgaag aagaggtcag ttgcttgtcc atccaagctc tcgtagtcaa
    12181 cacccttatt tgacttagca aatagaactg tcgcttcttt gacagcagcg tttttgctgt
    12241 gaggcattgc gattccatca cccaaaccag tagaagtcaa agcttcacgc gccaaaattc
    12301 cttctttaaa tgtttcaaaa tctgttacat aaccgtggtc tgtcaaattt ttaatcatct
    12361 cgtcgatgac agctgttttt tcagttgcct gcaaatctag caacatgaca tcttttctca
    12421 ataggtcttg aattttcatc gtttttctac ctcaactttt ccatatgttt ctttaataaa
    12481 ttccgccgtt gccaagtcat ctgagaaggt agttgccgtt ccgcaagcca ctccccattt
    12541 gaaggcttct actgcgtctt ttgatttgac aaattcacct gtgaatccag caaccataga
    12601 atcaccagct ccaactgaat ttttgactgt tcctttgatt ggtttagcga agtaagctcc
    12661 ctcagatgtg acaagaaggg caccatcacc agccatagag ataataacat tttgagcacc
    12721 cttagccagt aactcacgag cgtatttctc aatttcatct aaactttcga gtttaacccc
    12781 aaaaatcgct ccaagttcat gattgtttgg ttttacaaga agaggctggt agtccaaact
    12841 atcaattaag gtctgtcctt caaagtcaca gaccacttgc gcaccagtct ggcgcgtcaa
    12901 ggaaatcaaa tccttataga taacattgcc tagattttta gcacttgaac ctgcaaagac
    12961 aactgtatct tctgctgtca gactagataa aatagctttc aattcttcta gctgaaccga
    13021 ttcaacagtt ggacccgttc cgttgatttc tgtttcttgg tctgctttga ttttaacatt
    13081 gatacgagta tcttctgcca cctggacaaa acgtgtctcg atttcttcct ctgctaaagt
    13141 atctgtgata aatttaccag taaagccacc gataaatccc gtcgctgtat ttggtatatt
    13201 caaacgtttc aagacacggc tgacattgat tcctttccca ccagcaaact tatcatcact
    13261 gtccatacga tttacactac caactttgac ttggtccaaa cgaacgatat agtcaatgga
    13321 tggattgagt gtgactgtat aaatcatact tctattacct ccgttttctc cttaataacc
    13381 tgcaagagct catgcccttg actagtgata acgatagcgc gtttgagtgg tgctaccttg
    13441 gcaaagcaag tttgtccaat ttttgacgaa tccaccaaga cgtaggtctg cttggcattc
    13501 tccaaaatag ctcttttcac agctccctcc tccatatcag gagtcgtata atagccatcg
    13561 tcaacaccat tcattccgat aaaggcacgg tcaaagtgca attggttaat ctggttaaga
    13621 gcaacgcccc cgatactagc atctgtcgcc gtcttgacgt ttcctccaac catgacagtt
    13681 ggaatctgct tttcaaccaa ctgagcggca tggtgaatgg agttggtcac aactgtaaca
    13741 ttcttattga ccaattcatg aatcaaaaaa gcagttgttg ttccagcatc gataaagatg
    13801 acatcttttt ctttaatgag agaggctgct ttctgagcca gcaacttctt ttcttgaagg
    13861 tttttgacag atttttcttg aatggtttct tcttcctgta aggagtgggg gagttctgct
    13921 ccaccatgca cacgacgaag cttgttttcc gcttccaact catccaagtc tcttcgaacc
    13981 gttgattctg acgtttctag caaactaact aatttttcta gagaaactac atgatgttga
    14041 tttaactcct ctaaaatcag ttgtttacgt tctgttttta acactgaatc acctcctgtt
    14101 atcgtttaca ctattcattc taccatactt tatttcgaag tcaagcattt tttatcattt
    14161 tctttcaaaa tctatcattt tttcttattt ctagaatttt tattgcaaga taagagcctt
    14221 tatggtagac tatttgagta agtattggaa gattactcaa gaggcttaag aggccgtgtt
    14281 ggaaacgcgg taggcgtgta atagcgtgcg tgggttcgaa tcccatgtct tccgtggaaa
    14341 aaagattatt tttaggatgc aacaaatgtt gtatcctttt ttaaaatagg gtctgtttgt
    14401 gactcccttt ttagcaaaag aatgcaaagg aataccaaag ataaaaataa aaaacgttga
    14461 tttaacaacg ttttaccaag gaatgcaaaa gaatgcaaag gaataatgga gccggtggga
    14521 gtcgaaccca cgtccaaaca cctgccaaca tatttgtcta caaccatagg ttatgtatta
    14581 ttttaacagc ccctcgacac ataactcaag tctagaaact gcgagtctat caatctctta
    14641 tcaagccgct agacaaggct taatcgtatc tcgctaataa taagacctgt cattgaacac
    14701 gagcaatcca aatcgggtca cgcctgctgg tttttaggca gctagagcgt aagaagtgtt
    14761 attttttgca gttatattta actgagcgtt tacgtcgcca cacgagtcgc aaaatatgcc
    14821 tcataatgcc tgtcgaatcc gtaacgaccc caaaagacaa taaaaccatt ataccttatc
    14881 taactcaaaa atgcaaaaag aaatcacata cctaaaaaag atactattcg agaaatttag
    14941 caaactggaa gactaactac aactttctca gtgcataact ttacgattat aaatagagct
    15001 tacgaaatcc aattaacaca tacctaagat gaagctatct gttttgaaga aattcacgaa
    15061 gagaattatt ctaaaatact ttcagatgag aattttcgta ctctctttat tttaattcat
    15121 ttgagtttaa aaactataaa atcaatcctt atattattcg gactactttt cactta
//
LOCUS       DAVKWS010000045        15170 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKWS010000045 DAVKWS010000000
VERSION     DAVKWS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.045"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            221..1666
                     /locus_tag="VW852_001551"
     CDS             221..1666
                     /locus_tag="VW852_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV0272669.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
     gene            1668..2399
                     /gene="cps4B"
                     /locus_tag="VW852_001552"
     CDS             1668..2399
                     /gene="cps4B"
                     /locus_tag="VW852_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV0272670.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2408..3100
                     /gene="cpsC"
                     /locus_tag="VW852_001553"
     CDS             2408..3100
                     /gene="cpsC"
                     /locus_tag="VW852_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV0272671.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            3110..3799
                     /locus_tag="VW852_001554"
     CDS             3110..3799
                     /locus_tag="VW852_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV0272672.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTAPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            3814..5175
                     /locus_tag="VW852_001555"
     CDS             3814..5175
                     /locus_tag="VW852_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0272673.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            5181..5924
                     /locus_tag="VW852_001556"
     CDS             5181..5924
                     /locus_tag="VW852_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV0272674.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            5921..6745
                     /locus_tag="VW852_001557"
     CDS             5921..6745
                     /locus_tag="VW852_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV0272675.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            6747..7625
                     /locus_tag="VW852_001558"
     CDS             6747..7625
                     /locus_tag="VW852_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0272676.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            7626..8960
                     /locus_tag="VW852_001559"
     CDS             7626..8960
                     /locus_tag="VW852_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV0272677.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPILNTKIKVTDRLIDIQFWKIAL
                     TIIVDLIILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            8988..10412
                     /locus_tag="VW852_001560"
     CDS             8988..10412
                     /locus_tag="VW852_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272678.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            10477..11565
                     /gene="wecB"
                     /locus_tag="VW852_001561"
     CDS             10477..11565
                     /gene="wecB"
                     /locus_tag="VW852_001561"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV0272679.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            11728..12597
                     /gene="rfbA"
                     /locus_tag="VW852_001562"
     CDS             11728..12597
                     /gene="rfbA"
                     /locus_tag="VW852_001562"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV0272680.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            12598..13191
                     /locus_tag="VW852_001563"
     CDS             12598..13191
                     /locus_tag="VW852_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV0272681.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            13203..14249
                     /gene="rfbB"
                     /locus_tag="VW852_001564"
     CDS             13203..14249
                     /gene="rfbB"
                     /locus_tag="VW852_001564"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV0272682.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(14311..15165)
                     /gene="rfbD"
                     /locus_tag="VW852_001565"
     CDS             complement(14311..15165)
                     /gene="rfbD"
                     /locus_tag="VW852_001565"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV0272683.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
BASE COUNT         4628 a         2570 c         3194 g         4778 t
ORIGIN      
        1 ttagattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgat cgatttgtca tgttcttatt tcattttact atatttttgt ttcgcgggaa
      121 gtctactaag atacttaaag atgcagatag taaaaaaaat gtagacatta ccgtaaaaaa
      181 gtgatataat cgtatgatgt tcaaggtata ggtgttaatc atgagtagac gttttaaaaa
      241 atcacgttca cagaaagtga agcgaagtgt taatatcgtt ttgctgacta tttatttatt
      301 gttagtttgt tttttattgt tcttaatctt taagtacaat atccttgctt ttagatatct
      361 taacctagtg gtaactgcgt tagtcctact agttgccttg gtagggctac tcttgattat
      421 ctataaaaaa gctgaaaagt ttactatttt tctgttggtg ttctctatcc ttgtcagctc
      481 tgtgtcgctc tttgcagtac agcagtttgt gggactgacc aatcgtttaa atgcgacttc
      541 taattactca gaatattcga tcagtgttgc tgttttagca gatagtgaga ttgagaatgt
      601 tacgcaactg atgagtgtga cagcaccgac tgggactgat aatgaaaaca ttcaaaaact
      661 actagctgat atcaaatcaa gtcagaatac cgatttgacg gtcaatcaga gttcgtctta
      721 cttggcagct tataagagtt tgattgcagg agagactaag gccattgtct taaatagtgt
      781 ctttgaaaat atcatcgaat cggagcatcc agattacgca tcgaagataa aaaagattta
      841 taccaaggga ttcactaaaa aagtagaagc tcctaagacg tctaaggatc agtctttcaa
      901 tatctatgtt agtggaattg atacttatgg tccgattagt tcggtgtcgc gttcagatgt
      961 caatatcctg atgactgtca atcgagatac caagaaaatt ctcttgacca caacgccacg
     1021 tgatgcttat gtaccaatcg cagatggtgg gaataatcaa aaagataaat tgacccatgc
     1081 gggcatctat ggagttgatt cgtccatcca cactttagaa aatctctatg gagtggatat
     1141 caattactat gtgcgattga acttcacttc tttcttgaaa ttgatcgatt tgttgggtgg
     1201 ggtagatgtt tataatgacc aagattttac atctctacac gggaagttcc atttcccagt
     1261 tggaaatgtc cacttagatt ctgagcaggc tctgggattt gttcgtgagc gctattccct
     1321 agcggatggt gatcatgacc gcgggcgcaa tcaacaaaag gtgattgcgg ccatccttca
     1381 aaaattaact tcgagcgaag cgctgaaaaa ttatagtagg atcattgata gcttgcaaga
     1441 ttctatccaa acaaacatgc cacttgagac catgataaac ttggtcaatg ctcagttaga
     1501 aagtggtgga acgtacaaag tgaattcgca agacttgaag ggtatgggac ggacggatct
     1561 tccttcctat gcgatgccag atagtaacct ctatatgatg gaaattaacg acagtagcct
     1621 tgcatctgtc aaaactgcta ttcaggatgt gttggagggc agatgaaatg attgatattc
     1681 attcgcacat cgtctttgat gtagatgatg gtcccaagtc aagagaggaa agcaaggctc
     1741 tcttggcaga atcctacagg cagggggtgc gaaccattgt ctctacctct caccgtcgca
     1801 agggcatgtt tgaaactccg gaagagaaga tagcagaaaa ctttcttcag gttcgggaaa
     1861 tagctaagga agtggcgagt gacttggtca ttgcttacgg ggctgaaatt tactacacat
     1921 cagatgtttt ggataagctg gaaaaaaatc ggattccgac cctcaataat agtcgttatg
     1981 ccttgataga gtttagtatg aacacccctt atcgcgatat tcatagtgcc ttgagtaaaa
     2041 tattgatgtt gggaattact ccagtcattg cccacattga gcgctatgat gcacttgaaa
     2101 ataatgaaaa acgcgttcga gagctgatca atatgggctg ttacacgcaa gtaaatagtt
     2161 cacatgtcct caaatccaaa ctttttggag aacgttataa attcatgaaa aaaagagctc
     2221 agtatttttt ggagcgtgac ttggttcata tcattgcaag tgatatgcat aatgtggaca
     2281 gcagacctcc ccatatggca gaagcatatg accttgtttc ccaaaaatac ggagaagcga
     2341 aggctcagga actttttata gataatcctc gaaaaattgt aatggatcaa ctaatttagg
     2401 agaaatgatg aaagaacaaa acacgataga aatcgatgta tttcaattac ttaaaacctt
     2461 gtggaaacac aagctaataa ttttattagt ggcacttgtg acaggggcgg gagcttttgc
     2521 atatagcact tttattgtta agccagaata tacaagcacc acgcgtattt acgtagtcaa
     2581 ccgtaatcaa ggagataagc cgggactgac gaatcaggac ttgcaggcag gaacttatct
     2641 ggtaaaagac taccgcgaaa ttatcctttc gcaagatgta ttggaaaagg tagcgacaaa
     2701 tttgaaattg gatattccag caaaaacgtt aactagcaaa gtgcaagtga ctgtaccagc
     2761 cgacactcgt atcgtctcaa tctctgtcaa ggataaacaa ccagaggaag ccagtcgcat
     2821 tgctaattct ctacgagaag ttgctgcaga aaagatcatc gctgtaacgc gagtatctga
     2881 tgtaacgacg cttgaagaag cgcgaccagc tatgactccc tcttctccaa atgttcgacg
     2941 caacacctta gttggttttc ttggaggagc cgccgtaaca gtaattactg ttcttttgat
     3001 tgagttgctc gatacccgtg taaaacgtcc tgaagatgtt gaagatgtac tgcaaatgcc
     3061 acttctagga gtcgttccag attttgataa aatgaaatag gaggaagtta tgccaacgtt
     3121 agaaatctca caggcgaaat tagaacttgt gaaaaaggca gaggaatatt ataatgcttt
     3181 gtgcacgaac ctacagttaa gtggagatga tttgaaagta ttttctatca cttctgtgaa
     3241 agcaggagaa ggaaaaacaa cgacttccac caatatcgct tgggcttttg cgcgtgcagg
     3301 ttacaaaacg cttctgattg atgcagacat gcgtaactcc gttatgtcag gtgtctttaa
     3361 atcaagggaa aggattactg gactaacaga atttctatca gggacaacag acctgtcaca
     3421 ggggctttgc gataccaata ttgagaatct ctttgtgatt caggctggct ctgtgtcacc
     3481 aaatccgaca gcccttcttc aaagtaagaa tttcagtaca atgcttgaaa ccttgcgtaa
     3541 atattttgac tatatcgtcg tagacactgc ccctgtcggt gtcgtgattg atgcggctat
     3601 cattatgcag aaatgtgatg cttctatttt agtgacgaag gcaggtgaaa caaaacgacg
     3661 ggatcttcaa aaagcgaaag aacagttgga acaaactggg aagtcgtgtt taggagttgt
     3721 gttgaataaa ctcgatactt cagtagagaa atacggttct tatgggagtt atgggaatta
     3781 caggaaacag aaaaaatagg ttgagggata gaaatgagtg aaaaactagc aaagccttca
     3841 gtagccatag tccagagttt tcttgttatt ttattgactt atctacttag cgctgtgaga
     3901 gaaacagaga ttgtttcaac aacagctatt gtactttata tcctccacta ttttgtcttt
     3961 tatatcagtg attatggacg gaatttcttt aaaaggggat atttgattga acttgtccag
     4021 acattgaaat atatcctatt ctttgcgcta gcgattagta tttctaattt tttcttagaa
     4081 gatcgattta gtatttccag aagaggcatg atttacttcc tcttattaca tgttttctta
     4141 gtctatgtgc taaacccatt tatcaagtgg tattggaagc gggcttatcc caactttaaa
     4201 ggaagtaaga agattctcct acttacagca acttctcgtg ttgaaaaggt actggataga
     4261 ctaatagaat cagatgatgt tgttggggag ttggtagccg tcagtgtttt agataaacca
     4321 gattttcagc atgatgattt aaaggttgta gcagaggggg agatagtaaa ttttgcgact
     4381 catgaggtgg tcgatgaagt ctttatcaat cttccaagtg aaaaatacaa tattggagag
     4441 cttgtctctc agtttgaaac gatgggaatt gatgtaacag tcaatctaaa tgctttcgat
     4501 tgggcacgta acaagcaaat ttgtgagatg gcaggactaa atgttgtgac tttttctaca
     4561 acattttata agactagcca tgtgattgct aagcgggtta ttgatattgt cggttccctg
     4621 gtaggtttga tattatgtgg tctagtcagt attgtgctgg ttcctttgat tcgaaaggat
     4681 gggggttctg ctttttttgc tcagacgcgt ataggaaaaa atggccgcca ttttactttt
     4741 tataagtttc gctctatgtg tgtggatgct gaggagaaaa aaagagaact catggaacaa
     4801 aataccatgc agggtggaat gtttaaggtg gacgatgatc ctcgtatcac gaaaattggt
     4861 cattttatac ggaagacgag cttggacgag ctaccacagt tttataatgt tctaaaggga
     4921 gatatgagtt tggtaggtac acgaccacca acagtggacg agtatgagca ctatacccca
     4981 gaacaaaaac gtcgcctaag ttttaaacct ggcataacag gtttatggca ggtcagcgga
     5041 cgaagtgaaa tcaaaaattt cgatgaagtt gtcaaattag atgtggccta tattgatggt
     5101 tggacaatct ggaaagatat tgaaatttta ttgaagacag ttaaggttgt attgatgaag
     5161 gatggagcga aatagttcat atgaatgaga gaattcaaat attaggaatt actatagatc
     5221 cattaacgat gaaagaaaca gtggatgctg ttgaacagta tgttttaaag aagcaccctt
     5281 tgcacttgat gggagttaac gcggataaaa ttaatcagtg ctatgaagat gagaaaatca
     5341 aaaaaatcgt taatgaatca ggaataatta atgccgacgg tgcttcagta gttttagcga
     5401 gtaagttttt aggaacacca gttcctgagc gagttgctgg aatagacttg atgaaacatt
     5461 tactagagtt gtctaatgaa aaaggatact cggtctactt ttttggagca aaagaagacg
     5521 ttttacaaga tatgttaaaa atttttgaag aaagatatcc agctctaaat gttgttggct
     5581 atagaaatgg atatttttcg cctgaagatg aaaaacatat tcaggaagat attaaagaga
     5641 aaaaaccaga ttttgtgttt gtaggaatta cttctcctaa gaaagaatat attatccaat
     5701 cattcatgga taatggaatt aatgctgtgt ttatgggggt tggtggaagt tttgatgtac
     5761 tatcagggca tatcaaacga gctccattat ggatgcaaaa tgctcaccta gagtggttgt
     5821 ttcgtgtggc aaatgagcct aaacgtctct ttaagcgtta ttttgtaggg aatgtttcat
     5881 ttataagaaa agttttaaga gcgaaaaggg gatataaact ttgaaccaaa cagaaatagt
     5941 taaattgatt cagaaagttg aattggatgc tataaaagag tttcaaaaaa tttgtaaaga
     6001 gaacaatata gatttcttcc ttcgtggggg gagtgtactt ggggcagtca aatatgatgg
     6061 ctttattcca tgggatgatg acatggatat tgctgtgcct cgtgaaggct acgataaact
     6121 tccaggtatc tttaaggata gaattatcgc aggcaaatat caggttcttg cttatcaata
     6181 ttgcgatact ttacattgct actttcctcg cttatttctt ttagaagatg aaagaaaacg
     6241 tttgggattg ccacgaaata ccaatttagg tttgcatttg attgatatca ttcctttgga
     6301 tggtgcccca aatcattcat ttttaagaaa gctttacttc ggtaaagtat actggtatcg
     6361 ttttttagca agtttaggaa ctacctatgt tggagaccat gtggatatgc attccgctaa
     6421 gcaaaaacta atcattggtt tctttaaaaa gctaggattt gcaaaactat tccctcaaaa
     6481 ttatgtgtac agacgcttgg ataatctcta taaaaagtat gattggaaaa agcagaagta
     6541 tgctgggaca attaatgcat ctctgtttgc caaagaagtc atgccagtag agatttgggg
     6601 agaaggagta gagaagcctt ttgaggatgc cttctttaaa gttcccacgg agtatgaccg
     6661 ctatctgaaa agactttacg gagaaaacta tcttcacgaa gagcctagtg atgatgaaaa
     6721 gaaatcgcat ttaggaggac aataatttgt tttgttatat tattttgcat tacaaagtct
     6781 tagaggaaac tatttcttgt gttaaatcaa taaaagaagg caattccaat acaaagcaga
     6841 tcgttattat tgataatttt tctaataacg gtacgggtga aaaactacaa gagctgtatg
     6901 agtcagattc agagattgat gtcttgatta accatgaaaa tgctggtttc gctcgaggta
     6961 ataatgtagc atatcagttt gctaaggaaa agtacaaacc tgattttatg gttatcatga
     7021 ataatgatat tgagatagaa acagaagagt ttgaaaaaat cgtgacagat atctatcgga
     7081 aggaaaaatt ccatttgtta ggaccagata tcttttcgac gacgtatcag cttcaccaaa
     7141 acccaaaacg gttgacgcat tatacttatg aagaggttaa ggctctcaat gaaaaattta
     7201 agaaagggag ccaagttagt ctagcattaa aaattaaatg ttggttgaag tctagtaaag
     7261 ttcttcggac agcaatctat caaaataggc gtaaaaagaa atcagtagac tatagaaaac
     7321 aggtagaaaa cccaattctt catggttcgt ttattgtata ttctagagat tttattgaga
     7381 aagaggagta tgcttttaat cccaatacct tcttctatta tgaaacagag atattagatt
     7441 atgaagctga gttaaaagga tataagagaa tttatacacc gaagattaag gtcttgcacc
     7501 atcaaaatgt tgcaaccaat caagtctata caaacttagt agaaaaaacc ttgttttcaa
     7561 acaaatgcaa ctttgaatcc actagttatt ttttgaagtt gatgaaagaa aacgagggtg
     7621 tgtaaatgac ttatttattt ttactctgcc tgaccttatt cttattaact ttcttctatt
     7681 tttttgcttt caatcaagat ttgatagctc cgccagttgt catgtctgtt atgtttctca
     7741 ttagttctgt atttgccctt attaatgtgc aaaactggaa tattgagtat agtggtttag
     7801 cttatctttt gattattagt ggcattattg ttttttcaat gcctctcttg gctcttaatt
     7861 cgcctattct aaataccaag attaaagtga cggatcggct cattgatatc caattctgga
     7921 aaatagctct tactattata gttgacctca ttattctata tctttatagg agagagattc
     7981 ataatcttgc acttagccat ggttatacgg gttcaaattt tcagtggttc tttagaaatg
     8041 ctaccagtta tgaaggtgag ctaacagtgc gaacttcgat tcgggtcctc attcgtatca
     8101 ttgacgtatc tgcttatatt tttggatata cttttattaa taatttcttc atttatagtc
     8161 ataaacgctc taaagattta ctgctcttag ttccattctt gatttttatt tctaaaacct
     8221 tattatctgg gggtagattg gatattataa aaattttaat tgcgtatgtt gtaatggcct
     8281 atattcagca aaaacgaaaa gttggctggg ataaggtcat ctcccataaa tatatgagac
     8341 ttggttttgt aggcttgata gctgggattc ctacctttta ctattcttta ttcttatctg
     8401 gacgttctac gactagaact gtatttgaaa gtatttcaac ctatttagga ggttcgattc
     8461 agcattttaa tcagtatatt caaaatccta ttggagtagc tgaggttttt ggcgatgagt
     8521 catttgtagc tattatgaat attttgggta atcttggctt tgtcaattac aatagtaccg
     8581 ttcacttaga atttcggcag ttagggatta ctatgggtaa cgtttatact ttctttagaa
     8641 gaccctggca tgactttggg ttagtcggta tgtatatctt ttcctttgtt gtcggtgtgt
     8701 tttttgctat tttttatttg aaattaagaa agagccgtgc tggttttaaa ttggatattc
     8761 atacaattat ttactcttat ttcttttatt ggattttttt atcgtccatt gagcaatact
     8821 cgtttacaac aatcagtttg tttacacttg tatttatagt cttggtttac ctaatggctt
     8881 tcttctattg gaatttggat ttccacagag gaaaactagt aattaaactc tctgacacaa
     8941 gtattaaatc ggaaaactga aaacaataaa aatagagagg atagttgatg aatagcaaaa
     9001 ttaaaaatat actaactaat ttctcttatg tcatttcttc aaatcttttg acagtcttga
     9061 cctcttcctt ggttgtctta attttcccca aattaatggg ggtaacagag tacagttact
     9121 ggcaacttta tattttttat ctgacctata tcggtttttt ccacttgggt tggattgatg
     9181 ggatttatct taaatatggt ggcttagaat ataataattt agatagaaaa cagttttatt
     9241 ctcagatgat tctattttct agcttcttaa tgattatctc cttgctattg tttggtttaa
     9301 atgtattagc tgtaacagat tcgaatgcac gatatattta taatatgact attataagta
     9361 tgataatcac aaatttaaga acgctctacg tgtatgtttt gcagatgaca aaccgcttga
     9421 aggatagttc catcattcta ctcagtgatc gcgttttata tgtgctcctt ttattcctgt
     9481 ttattgtatt tggttggcat gaatacaagg ttatgatttt ggcagatatt ctaggtcgat
     9541 cattttctct catactttcc ttctggattt gtaaagatat tgtgtttcag cctttgtcaa
     9601 aattcatctt taatataaaa gagtcttttg ataatatccg agttggtatc aatttaatgt
     9661 tatccaacat cgcaagtagt ttgattatcg gtattgttcg tctaggaatc caatggaact
     9721 ggaatatcga aacattcggg aaagtatctc tgaccttgag tgtttccaat ctattgatga
     9781 ctttcattaa tgccattgga ctagtcgtct ttccgctatt aagacgaaca aaagcagaaa
     9841 acttacctaa gatttattct aatttaagaa acgttttgat gcttatcatg ttcgcgattt
     9901 tgctcattta ctatccttta aaaattgtat tagacctctg gttgccagcc tatcaagatg
     9961 ccttgatttt catgaccctt attttcccta tgtcagtcta tgaagggaaa atggcattgg
    10021 tcattaatac ttacttaaag gcattaagaa tggaaagaga tatccttaga gtcaatgcct
    10081 tagtaatgtt gatcagtatg ggagtgaccc tggttacaac atacctgtta aatagtttgg
    10141 agctgactgt tgtatcgata gttgttttgc tagctttgag aagtatcata gccgaattaa
    10201 ttctgtctaa aaaactggat gtttcggtta agaaggatat tgtattagaa tttcttttga
    10261 cgcttgtctt tatttcttca agttggtatt tgccgattgg gctggcagta attgtctaca
    10321 caatagccta cggtttatat ctctatttga aacacgaaga tatcaaaacc tatttagctt
    10381 actttaaagc tagtaaaaaa acatcaaatt aaaaaattat ataatgatta agtggtagat
    10441 tctatttcta ccgttttaga tattcgggag gtaatgatga aaaaaattat gttagtattc
    10501 ggtacacgtc cggaagccat caaaatgtgt ccattagtca atgagttgaa aaaacacgaa
    10561 gatatggaaa caattgtgtg tgttactgga caacacaaag agatggttag tcctgtttta
    10621 gatttatttg gtgttgtacc agattatgat ttagaaatta tgaaggctaa ccaaaccttg
    10681 ttctctatca caactagtat cttggaaaag ataaaaccag ttttagagaa ggaacaacca
    10741 gatattgtcc tagttcacgg tgacactacg acaacttatg cagcagcctt ggcagcattc
    10801 tatttgggaa ttaaagtagg acatgttgaa gctggtttgc gaacgtacaa tttacaaagt
    10861 ccatttcctg aagaatttaa caggcaatcg acatcaatca ttgcaactta ccattttgct
    10921 ccaactgagt tggctaaaga aaatctctta aaagaaggta gagagaatgt ttatgtgact
    10981 ggaaatactg tcattgatgc tcttacaact actgttcaag aggattatac acacactcat
    11041 ttagatttaa acgctaacaa tcgtctcatc ttattgactg ctcatagacg cgaaaatctc
    11101 ggcgaaccga tgagacatat gtttagagca gttaaacgag tattgaatga atacgaagat
    11161 gttaaagtca tttatccaat tcataagaat cctttggtac gtgaaacagc tgcagagatc
    11221 tttggagata cagaacggat tcaaattatt gaacctttgg atgttcttga tttccacaac
    11281 ttcatgaacc aaagttatat gattttaaca gattctggcg gagttcagga agaagcacct
    11341 tctttaggaa aacctgtatt ggttatgcgc gatactactg aaagacctga aggagtagcg
    11401 gcaggaacct tgaaattagt aggaactgac gaagaaacaa tttatcaaaa cttcaaacta
    11461 cttctagatg attctggaga atacaaaaaa atgagtcagg ctagcaatcc atacggtaat
    11521 ggtgatgcta gtcaacagat tgttcagatt ttgcgtggga tttaagaaga ttctctaaaa
    11581 gagtctaaga ggaatccact ccacaaacat aaaactctta tgctatcatt tatgattgag
    11641 atagactcat aacttaatat ttttatccta agaaaattcg ttcatattca atatgaatga
    11701 aaaacaacga agaaaggtta tttcattatg aaaggtatta ttcttgcagg cggctcaggt
    11761 acccgcctgt acccacttac tcgggctgcg tcaaaacagc tgatgccggt ttatgataaa
    11821 cctatgattt attatccgtt gtcgacatta atgttggctg gaattaaaga tattttgatt
    11881 atctcaactc ctcaagattt gccccgtttt aaggacttgc tcttggatgg ttccgaattt
    11941 gggatcaagc tttcctatgc ggaacaacct agtcccgatg gacttgctca ggcttttctt
    12001 atcggtgaag aatttatcgg tgacgatagc gttgccttga ttttaggcga caatatctac
    12061 catggtcctg gtctgagcac aatgcttcaa aaagcagcca agaaagagaa aggtgcgact
    12121 gtttttggct accaagtgaa ggatccagag cgttttggtg tggttgagtt tgatacagac
    12181 atgaatgcta tttctataga agaaaaaccg gagtatcctc gctccaacta tgcagtgaca
    12241 ggactgtatt tctatgataa tgatgttgtg gagattgcta aacagatcaa acctagtgct
    12301 cgtggcgagt tagaaattac agacgttaac aaggcttacc taaatcgtgg tgacctttct
    12361 gttgagctga tggggcgtgg ttttgcctgg ttggatacgg gaacccatga aagcttgcta
    12421 gaagcttctc agtatattga aacggttcag cgtatgcaga acgttcaagt ggcaaatcta
    12481 gaagaaattt cctatcgtat gggctacatt agtcgtgaag acgtgctgga attggctcag
    12541 cctcttaaga aaaatgaata cggacaatat ttgctccgtt tgattggaga agcatagatg
    12601 tcagataatt tttttggaaa gacacttgtg gtacgcaaga ttgatgctat accaggactg
    12661 ctagagtttg acattcccgt tcatggagac aatcgtggtt ggtttaagga aaatttccag
    12721 aaggaaaaga tgctaccgct tggttttcct gaaagcttct ttgctgcagg gaaactgcaa
    12781 aataacgtca gcttttctcg caaaaatgtt cttcgaggac tccatgctga accttgggac
    12841 aagtatatct ctgttgcaga cgatgggaag gttttaggat cttgggtaga tttgcgtgaa
    12901 ggcgagacct ttggaaatgt ttaccagaca gagattgatg ctagcaaggg aatttttgtc
    12961 cctcgtggcg tagccaacgg ttttcaagtc ctctcagata cagtttccta tagctatctg
    13021 gtcaatgact actgggctct tgaactcaaa cccaaatatg cctttgttaa ctatgctgac
    13081 ccagcattgg gaattgagtg ggaaaaccta gcggaagctg aggtttcaga ggcagacaaa
    13141 caccatcctc tattaaggga tgtcaaacca cttacgaaag acgagttgta aaaggaaaca
    13201 ttatgactga atacaaaaaa attatcgtga caggtggagc tggttttatc ggttctaact
    13261 ttgtccacta tgtttacaat aactttccag atgtccatgt gacagtgctg gacaagctga
    13321 cttatgcagg taatcgtgcc aatatcgagg aaattttagg cgaccgtgtt gagttggttg
    13381 ttggagatat tgctgatgca gccttggtag acaagttggc ggctgaagcg gatgctatcg
    13441 ttcactatgc ggcagaaagc cacaatgata actcgctcaa tgacccgagt ccatttatcc
    13501 acaccaactt tatcggaact tacacacttt tagaagcggc tcgtaaatac gacattcgtt
    13561 tccaccatgt atcgactgat gaagtctatg gtgacctgcc tctgcgtgaa gatttgccag
    13621 gtcatgggga aggcctaggt gagaaattta ccgctgaaac caagtacaat ccaagctcgc
    13681 cttactcatc aaccaaggct gcttcagact tgatcgttaa agcttgggtg cgctcatttg
    13741 gtgttaaagc aacgatttct aactgttcaa acaactatgg tccataccag catatcgaga
    13801 agttcattcc gcgccagatt acgaatatct tgagcggtat caagccaaaa ctctacggag
    13861 aaggtaagaa tgtgcgtgac tggattcaca ccaatgacca ttcatcaggc gtttggacga
    13921 ttctgaccaa gggtcaaatc ggtgaaactt acttgatcgg tgctgacggt gagaagaaca
    13981 acaaggaagt gttagagctc attctcaagg agatggggca acccgctgac gcttatgacc
    14041 atgtgaccga tcgagctggt cacgacttgc gttatgcgat tgatgctagc aagctccgtg
    14101 atgagttggg gtggaagcca gagtttacca actttgaagc aggcctcaaa gagaccatca
    14161 agtggtacac agataaccaa gactggtgga aatctgaaaa agaagcagta gaggctaact
    14221 atgctaaaac gcaacaagtg attaaataat aagatactaa aaaagcaaga gacctcgagg
    14281 tctcttgctt ttctagtatg tagtaagctt ttattttctt acttcttgtt tataaaactc
    14341 tttaagggca tcttgccagg ttgggatgac gaaacctgtt gcctttgcct ttgccaaact
    14401 catagttgag ttgagaggtc gtttagcctt agcggggaac ttgctagagt ctactggcac
    14461 tacttcaaca tcactgtcct taagaatctc gctagcaaag tcataccaag tggtgtcttc
    14521 agctgcgtca tttgataagt gatagtagcc atactctttt tgattttcag tcacataggt
    14581 catgaattcg gccaaggtcc gtgtccaagt tgggcgaccg tattggtcgt tgaccactgt
    14641 gagcgtttta tgggttttgg ctaggctttg catggtaaag acaaagtttt ttccataatt
    14701 tccaaaaacc caagcagtac gaatgatgta atgctgtgac gtaaggtttt caacaagttc
    14761 ttctcccatt cgcttggtac gtccgtactc tgtttgcgga tcaggtatgt catcgacttc
    14821 ccactcttgt cctactggtt tctttccatc aaagacgtag tctgttgaga tatagaccag
    14881 aatagctccg tatttctcag aggccttggc tacattttca gttccagtta cgttgatggc
    14941 aaaatccagt tctttccctt catcttcggc tgcatcaaca gcagtgtagg ctgcacaatg
    15001 atagactaga gttggcttaa cctcagcaaa gactttttca accatttcag aattagtgat
    15061 atccatttcg gtcacatcca ctgcaacata gtccacattt cgctcatcta gtaaataacg
    15121 tagttcggta ccaagttgac catttgcacc tgtaattaag atcatatttt
//
LOCUS       DAVKWS010000046        14440 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKWS010000046 DAVKWS010000000
VERSION     DAVKWS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.046"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            19..514
                     /locus_tag="VW852_001566"
                     /pseudo
     CDS             19..514
                     /locus_tag="VW852_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227923.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
     gene            768..1064
                     /locus_tag="VW852_001567"
     CDS             768..1064
                     /locus_tag="VW852_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0272684.1"
                     /translation="MKTSKIILSVLTVIISCSLACPVFADWVSGGNWSYGGYHNPGNW
                     GAFSNYFHDYRWHWSSVTRASDSKANVGYASAHYTSRSFINTSFGETAYFNYGF"
     gene            1126..3135
                     /locus_tag="VW852_001568"
     CDS             1126..3135
                     /locus_tag="VW852_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEV0272685.1"
                     /translation="MKRLFIHFSNLFILIFLLWIAFISPNTVIHRSLPVIGILQQERE
                     VVYEELSSSLDQLAKENNSLIARQIQKTDSKGQVKFSYDIYGEGALPNGIKKEEKEFV
                     AKESLLTNYYILSGNLTLEKLDQKLHDLGFSKSFMNKPNPLQNFMVFFSSGSQSLALV
                     IFIISFAALTIIQKTLEMRSAGIRYISGIRRYQLFGHSLMEDGKELFLGCIGGSVLGA
                     ILIYYLQLTPFAYSLIISASIIYNTLLFILSAFLSFFFAFSIQTVHLVSLLKGKIPLK
                     RVLFFLFTCQFLAITVIGLSIHRVSIYGSIWQTYQEGSLAWSKETNWIQISLNRENVL
                     QTTNKEMQIEQIAKWSKLIESGIEKGGLLVHHQLAAFNSKGFMNDPRTGRELSITDYD
                     PLANTLYVTPNYLDIQRILVSPEEKERLNHLQAGEFGLLLPEKLKGQEEELKKRYEDY
                     LTPSDEQGKSQLPMKARVTYLPNNQKRFIYNNTPMIYQQFLTDPILVVVLPRSFGGYD
                     NPYFSHLNSYLYFDGLEKSKKLVVENGLEKNVSQYDYAASVYQQMIQSIQLENLITIA
                     GGIFAIATSILLFNTMNFLYFEEFRRPIFLKKIAGLGFVKIHQMILLSESILLLLGSF
                     LTFLLTQEWWIALVTLLLFITNAWFILLYRSHKEDHLLATVLKGA"
     gene            3137..3769
                     /locus_tag="VW852_001569"
     CDS             3137..3769
                     /locus_tag="VW852_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bacteriocin export ABC transporter"
                     /protein_id="HEV0272686.1"
                     /translation="MISIEHLTKSFGQRTVFQDLSLQFTEGKVYALIGNSGCGKTTLL
                     NILAKLEPYEKGSISYQGQELKQIKQHHFFKHELGYLFQNFGLLENETIATNLDLGLI
                     GQKLTKKEKKQQEEEVLKKVGLAYLALDQKIFELSGGEAQRVALAKVILKDPPLILAD
                     ELTAALDPETSQEVMDLLLMLKNQERIIIIATHNPDIWNQADEVINLNQL"
     gene            4363..4662
                     /locus_tag="VW852_001570"
     CDS             4363..4662
                     /locus_tag="VW852_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272687.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKRDYIEFKR
                     VGIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            4652..5140
                     /locus_tag="VW852_001571"
     CDS             4652..5140
                     /locus_tag="VW852_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272688.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNDPEKLADFIMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            5140..7017
                     /locus_tag="VW852_001572"
     CDS             5140..7017
                     /locus_tag="VW852_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEV0272689.1"
                     /translation="MYSGKKFILFSLLGILLGYLFHRLTLLYDSYTGNTLDKWIHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFLHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYKNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVISDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWRYLANQESDERWWNYQINPLNQNQENHLEGLRIRDLTFESSL
                     K"
     gene            7038..7280
                     /locus_tag="VW852_001573"
     CDS             7038..7280
                     /locus_tag="VW852_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272690.1"
                     /translation="MFTHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            7297..8151
                     /locus_tag="VW852_001574"
     CDS             7297..8151
                     /locus_tag="VW852_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEV0272691.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSNYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTLSGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGLGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            8205..8564
                     /locus_tag="VW852_001575"
     CDS             8205..8564
                     /locus_tag="VW852_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEV0272692.1"
                     /translation="MNTRVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLLLFGVYKPNDLYFEHYLKYRLHFELTIPLRTISGKKGL
                     EHEKKIKYIKETKDFND"
     gene            8515..10872
                     /locus_tag="VW852_001576"
     CDS             8515..10872
                     /locus_tag="VW852_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0272693.1"
                     /translation="MKRKSNTLKKQKTSTTNKKEEVKDKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDQTNFQLTIFNKR
                     LNLEKFRHSVLYEEKEDGYDSYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFGSLTGEERVNLLADMLRGEHHLPFSYRDLTRSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGVFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNIAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRAFGGEMIDIAP
                     DSQTYLNVLDLSEENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQPEEEAQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            10884..13697
                     /locus_tag="VW852_001577"
     CDS             10884..13697
                     /locus_tag="VW852_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV0272694.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKQGKKEVRKQKKDSAVLDE
                     KAWKNEIKQKLENMREASKARVKQANEDYNHILQNSPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFKEAKVEAKEERKESRKERKTNQKFFYGQETKVKSNFFLQGKNLEELKAKKE
                     VKAAKENLKSTKQAYKFKKISRKAKTFLYVLGREGGELASENEDLESYRTLHETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLTKDNPSSFKNR
                     YRKLKKQGLLSVRNIYQKLKGAFSFFTFVAGNPVIWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELQKPV
                     HLFSSETYKDYLSALWHDLNDGEDLKSMQDLYETPKYKLSKDDQEEIKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDGVVSL
                     DGDDVILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDVIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYDFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDETWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYARTQNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYEHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPAIYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISHADSAISFAYTTK"
     gene            complement(13728..13916)
                     /locus_tag="VW852_001578"
     CDS             complement(13728..13916)
                     /locus_tag="VW852_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272695.1"
                     /translation="MVYRVCKPPSQSVYSGVCHRLALTSKIAIGFRSGVRHRLALTSK
                     ITIRLTMSRPLIYTIYFT"
     gene            13936..>14440
                     /locus_tag="VW852_001579"
     CDS             13936..>14440
                     /locus_tag="VW852_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272696.1"
                     /translation="MANSRDYRNPNYTETIKLQRFFTQLQIAASFFKEHFVGKIMYYE
                     TEIESVELHFSPTNFMHLCGVDYRKGAGSFFDDCLNRHVIIDELKIKKDGTTMQKLQV
                     LGSIGELLGKHVHLTGSGRYLYLEFDYALRTRKQIIALTLKETSRKIVPQSLLDLKRK
                     TVFPKGQK"
BASE COUNT         4762 a         2394 c         2926 g         4358 t
ORIGIN      
        1 ctaagaaagg agaatgaaat ggttgataaa agagaaaaac tgatgaactc tttcaatcag
       61 tatggttttt taacttttaa acaagtaata gatgaaaatt tacactacaa aaccttattg
      121 aaaatgcttg cagaaggaaa aatcgatgct gaagaaaaag ggttatatcg cttacctgat
      181 atttatttag atgagtggtt tgtccttcag tatcgatttc caaagggaat cttttctttg
      241 gagacagcac tttggttaca tggtttatct ttgactatcc cttttaacat gacgatgagt
      301 tttccttatg gtacgaatac caaaaacatt aaggaagcag atatatgtcc tattatttta
      361 cgctctcact atagtgaagg aattattgaa atagagcgcc ttcctggtca atttattaaa
      421 gtttatgaag ttgaacgagt tttggttgaa tgtctatgcc cagctattta aagtaactga
      481 taagttacaa tcttatacgg aggtgctatc ttaatgtttt ttaatacaaa aactttaata
      541 gttttagaat ttggttgata gcttatgaat aaacagctat attgttttga ttattaaata
      601 ttcaaaaatc tattatataa ttagttgaaa ttatatgttg taagtgttat aatgcaggcg
      661 aaaagggttg ctctggaaag gagaaaaaac ggaaactata ttttgagcaa ttcataattt
      721 tattgttaaa taaagctttt aattctaatt atggaaggtg aacagtcatg aaaacgagta
      781 aaattatcct ttctgtctta actgtaatta ttagttgtag cttagcgtgt cctgtatttg
      841 ctgattgggt atctggtggt aactggagtt acggaggtta tcataatcca ggcaattggg
      901 gagcattctc aaattatttc cacgactatc gatggcattg gtcaagtgtg actcgagcaa
      961 gtgacagcaa agctaatgta ggttatgctt ctgctcatta cacatcaaga tcgttcataa
     1021 atacaagttt tggtgaaact gcatacttta attatggatt ttaattgcta aagtttatcc
     1081 tagagagaag aaatctctct agggtaaact agaaagagta gaattatgaa acgtttattt
     1141 atacatttct cgaacctttt tattctaatc tttctgcttt ggattgcctt tatttcacct
     1201 aatacggtca tccatcgaag cctacctgta atagggattc ttcaacaaga aagggaagtt
     1261 gtttatgaag agctttcatc tagcttagat cagttagcaa aagaaaacaa tagtttgatt
     1321 gctcgtcaga ttcaaaaaac ggattctaaa gggcaagtca agttttcgta tgatatctac
     1381 ggagaaggag cacttcctaa tggcatcaaa aaagaagaga aagaattcgt tgcaaaggaa
     1441 agtttgctta ccaattacta tatactatct gggaatttaa cccttgaaaa attagatcaa
     1501 aaactccacg atcttggttt ttcaaaaagt ttcatgaata agcccaatcc cctgcaaaat
     1561 tttatggtat tttttagttc gggttcccaa tctctagcct tggttatttt tatcatcagc
     1621 tttgctgcat tgaccattat tcaaaaaaca cttgaaatga gaagtgcagg gattcgttac
     1681 atttctggaa taagacgata ccagctcttt ggacattctc tcatggaaga cggtaaagaa
     1741 ttgtttttag ggtgtattgg tggaagtgtc ctaggagcta ttctgattta ttatttacaa
     1801 ttgactcctt ttgcctattc tctcatcatt tcagctagta ttatttacaa tacactttta
     1861 tttattttat ctgctttcct atcctttttc tttgcattca gtattcagac agtacacttg
     1921 gttagccttt taaaggggaa aattccatta aaaagagtct tgttttttct ctttacatgt
     1981 caatttcttg caatcactgt cattggtctc tcaattcatc gtgtcagcat ctatggctct
     2041 atctggcaga cttatcaaga gggaagtttg gcttggtcta aagagacaaa ttggatacaa
     2101 attagtttga atcgggaaaa tgttttacaa acgacaaaca aagaaatgca aattgaacaa
     2161 atagctaaat ggtctaaact gatcgagtct ggcattgaaa aaggtggact tttggtccat
     2221 catcagcttg ccgcatttaa ttcaaaaggc ttcatgaacg atcctagaac agggagagag
     2281 ctttcgatca cagattacga tcctcttgcc aacaccctgt atgtaacacc aaattacctt
     2341 gatatccaaa ggatcttagt ttcccctgag gaaaaagagc gcttgaatca cttgcaagct
     2401 ggagaatttg gactcttgct tcctgaaaag ttgaaagggc aagaagagga gttgaaaaaa
     2461 cgctatgaag attatctgac tcctagtgat gaacaaggaa agagtcagtt gcctatgaaa
     2521 gcacgggtga cctatcttcc taacaaccaa aaacgattta tctataataa tacaccgatg
     2581 atctatcagc aattcttgac agacccgatt ttggttgttg ttctacctag aagctttggt
     2641 ggctacgata atccttattt ctctcatctt aattcttatc tttactttga tggtctggaa
     2701 aaaagcaaga aactagtagt tgagaatggg cttgaaaaaa atgttagtca atatgactat
     2761 gcggcgtctg tataccagca gatgatccaa tccattcaat tagagaatct cattaccatt
     2821 gcaggaggaa tctttgcaat agctacttcc atactactct ttaacaccat gaatttcctc
     2881 tattttgaag agtttagaag accaatcttc ttgaagaaga ttgcaggtct gggatttgta
     2941 aagattcacc aaatgatttt gctctcagaa agtatcctct tactattagg aagcttcctg
     3001 acattccttc tcactcaaga atggtggatt gctcttgtca ctctcttgct atttattact
     3061 aatgcttggt ttattctcct gtaccggtct cataaagaag accacttgtt agccactgta
     3121 ctgaaaggag cctaatatga ttagcataga acatttaacc aaatcatttg gccaacgaac
     3181 ggtctttcaa gatttgagct tgcaatttac agaaggtaag gtctatgctc tgatcggaaa
     3241 tagcgggtgt ggtaaaacaa ccttgcttaa tatcttggca aagttagagc cttatgaaaa
     3301 gggaagtatc agctatcaag ggcaagaatt gaaacaaatc aaacagcatc acttctttaa
     3361 gcatgaatta ggctatctct ttcaaaactt tggcctactt gaaaatgaga ctatcgctac
     3421 aaatttagat ttgggattga ttgggcaaaa attaacgaaa aaagagaaga agcagcaaga
     3481 agaagaagtg ctcaaaaaag taggattggc ttaccttgct ctggatcaaa agatttttga
     3541 attatctggg ggagaggcgc aacgtgtcgc attagctaaa gttattttaa aagatccacc
     3601 tttaattttg gcagatgaac taactgcagc actggatcca gaaacctcac aagaggtgat
     3661 ggacttgctc ttaatgttaa agaatcaaga gcgtataatc attattgcaa cccataatcc
     3721 cgatatttgg aatcaagcag atgaagttat aaatttaaat caattatagt tacagtcaat
     3781 agatatcttc tatattttaa agttatttta tctgaatccg taaaagcagt tcaattgaac
     3841 tgcttttttc ttctacgact tacactgttt ggtttcatga gcaagcccct caaagtgaat
     3901 taataacaaa tcattagaag tcatagtgat gaaatttctg taacggaagt agagcgaatt
     3961 gctgaaccat taaatgagta tctctcgaag tagaagcgtc tattttgact tttagtttga
     4021 tagaatggta gtttgaaaat gtaaatgata gctactgttc ataaaacata taaggtacgt
     4081 acggtgttgt gagaggagct agaaattagc acttacctta ttattccaac aaccccaaaa
     4141 atgtgataaa aatttcaact actcttatct ataataaagc tacctgttct ttgaaaattg
     4201 aatccactcc gtcttgatta gcgtgcctgt gtaagtaggg actgagagta tttccctgtg
     4261 aagggcgact ggcacaagac gtgtgtgaga attttctaac agacacggag tttatcgtta
     4321 ccagacttaa acgatgcgac aaactagata aaggagttaa tcatgaaggt agtaaacttg
     4381 tatgatttga aacaaatggg aaataaaggt ggttgtacca tccaattgat tcatcacttt
     4441 ccttttggta tgggcttagg acatctcaaa agagactaca ttgaatttaa acgtgttggt
     4501 atctttgatg ggaaagcagt agaagttact cttcgagaac cttattcgag agatctactg
     4561 caggttgtca agtcgattaa gcaacgtcag aaattgatag cttatcgtta taaagaagga
     4621 aagctgctat ttgttaagaa ggaggcatca gatgtcctct gaacaacagg aacgaatggc
     4681 agttcaatat gctgagcgta gtcttttatt tactgtaaaa agtcttttaa agattctaga
     4741 atggtctaga cgtcaggctt tagcacaaga ttccgcctat aagatagggg tgcagaaatt
     4801 agaagaattg ctacaatctc cctattcgat tgatacgatt aatctgaaaa aagatttttt
     4861 agacaaacca attgatatag agaaatttaa agctttttta gaaaaagaag agattccttt
     4921 agccatcgct tggcaagggg attctctgca tttctacacg aaagaccgtt cgattctaga
     4981 caatcattta gaccatctgt tagaaaaaat ggttaatgat ccggagaaat tagctgattt
     5041 tatcatggat aagtcattag acgatgcaat tgatgaggct aaatcccaaa ttacctttag
     5101 acaagaaggt gccgtcaaac agaaagagat ggtgagatga tgtacagtgg aaagaaattc
     5161 atactattct cactgttagg tatcttacta ggctatcttt ttcatcgttt gacgcttttg
     5221 tatgattcct atactggaaa tacattagat aaatggattc atcttctgat ggaaggtcaa
     5281 gatgaagttc ttcagtcgcc atggaatgtt tcctttactg gaaaatcaag tgcttttttt
     5341 ctactaggct ttgtgatgat gttgctggtt tatctctatc tagagactgg taaaaaacaa
     5401 taccgagaag gggtagaata cgggagcgcc cgttttggaa ctctaaaaga aaagaagctc
     5461 ttttacggta aggaattttt acatgatacg atcttagcac aagatgttcg tttgacatta
     5521 ttagataaaa aaccacccca atatgataga aataagaata ttgcagtgat cggaggttca
     5581 ggaagtggga agacatttcg ctttgtgaaa cccaatctaa ttcagatgaa tagttctaat
     5641 attgtagtgg atcctaaaga tcacttggcc gagaaaacag gaaaactctt tttagaacat
     5701 ggctaccaag taaaggtgtt agatttagtc aatatgaaga actcagatgg cttcaatcct
     5761 tttcgctata tagagacaga aaatgatttg aatcgcatgc tgacggttta tttcaataac
     5821 accaaaggct ctggctctcg tagtgatcca ttttgggatg aagcttctat gactttggtc
     5881 cgagctttag cctcctactt ggtcgatttc tataatccac ccaaaacaag agaacagctc
     5941 atagaagaaa gtcgtttaag tcaaaaagaa tacaaaaact tgttgaaacg tcaaaaaaaa
     6001 gaagtggagg agcgaaaaaa acgagggcgt tatccaagtt ttgctgaaat ctcaaaactc
     6061 attaaacact tatccaaggg tgaaaaccaa gaaaaaagtg tcttagaaat tctatttgaa
     6121 aattatgcta aaaagtatgg gactgaaaat tttaccatgc gaaattgggc agattttcaa
     6181 aattataagg ataagactct ggattctgtt atagctgtaa ccactgctaa atttgccctc
     6241 ttcaatattc agagtgtcat ggatttgacc aaaagagata ctcttgatat gaagacatgg
     6301 ggcaaggaaa aatcaatggt ttacttagtt atctcagata acgatagtac ctttcgcttt
     6361 ctttcagccc tctttttttc aacagtattt caaactctaa caagacaagc agatattgat
     6421 tttaaggggc aattacctct tcatgtgaga gtctacttag atgaattcgc aaatatcgga
     6481 gaaatcccag attttgctga acaaacctca acagtccgtt ctcgaaatat gagtctcgtt
     6541 cctattctac aaaatattgc ccaacttcaa gggctctata aagaaaaaga agcttggaaa
     6601 accattcttg ggaactgtga tagcttagta tacttaggtg gtaatgatga agataccttt
     6661 aaatttatga gtggtttact cggtaaacaa accattgatg ttcgaaatac tagtcgttcc
     6721 tttggccaga caggttcagg atccctttct catcaaaaga ttgctcgtga tctaatgaca
     6781 cccgatgagg tcggaaatat gaaacggcat gaatgcttgg ttcgaattgc caatatgcct
     6841 gtctttaaaa gcaaaaaata caattcaacc aagcatccaa actggaggta cctagccaat
     6901 caagaaagcg atgaacggtg gtggaactat caaatcaatc ctttgaatca aaatcaagaa
     6961 aatcatcttg aaggccttag aattcgtgat ttaacttttg aatctagttt aaaataaaaa
     7021 tagaaaagag gaaatacatg tttacgcatt ttaaaggttt tgtttatgga gtagacgcaa
     7081 gtgctatgtt tgcacaagct atgtctttgt tacagaaggg attgattgcc gttggtgcct
     7141 ttctcgttgt tgtggggatt gtcaatcttg caaccaacat taaagatgga ggaccaggtg
     7201 ttcggaatgc cattcttgaa attgtcggtg gagttatggt cggggctgct ggagcctttg
     7261 taacccagat ttcaatttag gaggataaac aatgacatga atcttagttt agtctcaccc
     7321 tttgtttacc ttgcatctga aaaaatatca gctgaaaatt tatttgaagg atttaatgta
     7381 gatttacaat ctacggtaga tctgattaaa tcactatcta actacaatcc aaccgtttgg
     7441 acttatatgt ctagtattac taaaagtgtc atgcagcccc ttggagttgc gattttatcg
     7501 gttgttctca tcctagaatt ttcgaagatg gctaagaaaa ttgctaactc tggtggagcg
     7561 atgacctttg aagccttagc gccaatgctg attagttata ttatggtcgc agttgtaatt
     7621 accaacacta ccgtcattgt agaagccatc atcgggattg cgagtcatgc cattgaacaa
     7681 gtggcctcga ttgtggctca cggtggggca aagtatgata cactctctgg attaaaaggg
     7741 tcaggattta ttggccggat gattgtgggc tttttcgccc tcctcatttg gcttgttcgg
     7801 atagtaagtg cagccatggt taatcttttg gtatctattc gatttattca actctacctt
     7861 atgatcccat ttgcccctct tacgattcca acatttttaa gtgatgaatg gaagtctatt
     7921 ggtttaggct atttaaaaaa tattatggtc tatgcggtac aaggggttct tatttttctg
     7981 attgtttctc ttgttccttt gtttgaatct gctggtaaaa tagctgtttc aaatggtgca
     8041 ggagtcctgc aatcacttgc gattatgttt ggtagtttgg tacaagctat cttactgatt
     8101 attgccctcg ttggttctca acgtacggct cgctcaatct taggtatgta attagataaa
     8161 ggctaggaag tggttgcttc ttagcctttt tagaaaggaa agtcatgaat acacgtgtct
     8221 ttaaagacat ctcaaaatac caacacaggg cttggttagg tttcaccaca agacaaatca
     8281 tctttgtttt accagccttt attgtcacaa ttattgtttt gggcttgaat ctctttttct
     8341 ggcaatttgg agattggttt gtttacggtt ttgtgtttgc ttttaccatc cccctcttgc
     8401 tttttggagt ctataaaccc aatgatttat attttgaaca ttatttgaaa taccgtcttc
     8461 attttgaact aacgataccc ctacgcacaa tttcaggaaa gaaaggactt gaacatgaaa
     8521 agaaaatcaa atacattaaa gaaacaaaag acttcaacga ctaataaaaa ggaagaagtt
     8581 aaagataaaa aagaggaagt gttaccatca acggctaata ctctttccta tcaagccttg
     8641 tatcaaaatg gtctgatgca ggtaaaagaa gattattttt cacaaagcta tttacttggt
     8701 gatgtcaatt accagaccgt tggtttagaa gataagggag caatcattga gaagtattct
     8761 gatttgatta actctttaga tgaccaaacc aacttccaat tgaccatttt taataaaaga
     8821 ttgaatttag aaaaattcag acacagtgtt ttgtatgagg aaaaagaaga tgggtacgat
     8881 agctatcgta aagaattgaa tcggatgatg aatcaaaatt tagatagtgg tgaaaataac
     8941 ttttcggctg tgaaactgat tagttttggt agaaaggatt ctaatcccaa acaagcttat
     9001 cgttccttgt ctcaaatagg cgaatatttc aagagtggtt tctcagaaat tgatgctcga
     9061 tttggatcct tgactggaga agaacgggtg aacttgttgg cagatatgct tagaggagaa
     9121 caccatcttc ctttttctta tcgtgattta acgagatctg gccagacaac tcgtcacttc
     9181 atagcaccta atctcttgga ttttaaaaac aagaattacc tacaaatcaa tgaccgctta
     9241 ttacagattg tctatgtgag agactacggt atggaattag gggatcagtt tatccgagac
     9301 ctcatgcaag gagatctgga attgattgta agccttcatg ctcaaagttc gaccaaggca
     9361 gatgccatga agaaactacg aacaaagaaa accttaatgg aatcccaaaa gattggggaa
     9421 caacaaaaac tagctcgtac aggtatctat ttggaaaaag taggtcatgt attagaaagc
     9481 aatatcgatg aagctgagga actcttaaaa accatgaccg agacaggaga taaactattt
     9541 caaacggtct tcttgattgg ggtctttggt caggatgaag aagaactcaa acaagcccta
     9601 gacactatcc aacaagtggc cggctcaaat gacctaatga ttgataaact tccatatatg
     9661 caagaagcag cctttaatag tttgctgcca tttggttgtg attttttaga gggagtatca
     9721 cggagtttat taacgtccaa tatagcagtg aactcacctt ggacttcagt agacttacaa
     9781 gaccgtagtg ggaaatatta tggtatcaat caaatctcaa gcaatattat taccattgat
     9841 cgcagcctat taaatacacc gtctggtctg attttaggaa catctggagc tgggaaaggg
     9901 atggcaacta agcatgaaat tatcacgacc aaaatcaagg aatctggtga aaatactgaa
     9961 attatcatcg tggatccaga agcagaatac agtgtcattg gacgagcttt tgggggagaa
    10021 atgattgata ttgcgcccga ttcccaaact tatctcaatg tccttgactt gtctgaggaa
    10081 aatatggatg aggatcctgt aaaggtaaaa tcagaatttc ttttatcttt tattggcaag
    10141 ttattggata gaaaaatgga tgggagagaa aaatcgatta tagaccgagt caccagactc
    10201 acctatcagt catttaaaga gccttctttg gaagaatggg tctttgtctt gagccaacaa
    10261 ccagaagaag aagcgcagaa tttggcactt gatatggaac tgtatgttga aggttctctt
    10321 gatatttttt ctcataagac caatattcag acaggatcta acttcttaat ctataacgtt
    10381 aaaaagttag gagatgagct gaaacaaatc gctcttatgg ttgtttttga tcagatatgg
    10441 aatcgtgtcg ttcggaacca aaaattaggg aagaagactt ggatttattt tgatgaaatg
    10501 cagcttcttt tattagataa atatgccagt gatttcttct ttaaattgtg gagtcgtgtc
    10561 agaaaatatg gagccagtcc gactgggata acccaaaatg tcgaaacctt attgttagat
    10621 ccaaatggta gacggattat tgccaatagt gaatttatga ttctcctcaa gcaagcaaaa
    10681 aatgaccgag aagaactagt tcaactctta ggcttgtcaa aagaactcga aaaataccta
    10741 gtcaatccag aaaaaggggc aggactaata aaagctggtt cagttgtcgt tccctttaaa
    10801 aataagattc cccaaggtac acaattgttt gatatcatga gtacagatcc tgataaaatg
    10861 gcttctaatt aaggggaagg taaatgaagg ataagagaga aatcatacgt gcccgaaagg
    10921 catttagaag aagtctaaaa gatgagaaga aattcttaaa acaaggaaag aaggaggtga
    10981 gaaaacagaa aaaagattcc gctgtactgg atgaaaaagc atggaaaaat gagataaagc
    11041 aaaagctgga gaatatgaga gaagcttcaa aggctagagt aaaacaagca aatgaagact
    11101 acaatcatat tcttcaaaat agtcctccat cacttttgaa tcggaaagaa ttaagagata
    11161 gacgattgcc tcatgctagg aaacgattga aaatagccaa gaaacaattt aaggaagcta
    11221 aggttgaagc aaaagaagaa agaaaagaga gtcgtaaaga aagaaaaacc aatcaaaaat
    11281 ttttctacgg tcaggaaacg aaagtaaaat ccaatttttt ccttcaaggg aagaatctag
    11341 aagaattaaa agctaagaaa gaagtcaaag ccgcaaaaga gaatctaaaa tctactaagc
    11401 aagcctataa gttcaaaaaa atcagtagga aagccaaaac ctttctttat gtccttggac
    11461 gtgaaggtgg agagttagct tcagaaaatg aagatttaga aagttatcgc acacttcatg
    11521 agaccattag aaaagggaaa cgctacagtc ggctttctta taatcttgga aaagctagtg
    11581 tcaaaacagg acaagcaaca ggtcgtttta ccaagaaaag actgaccaac acaaaagagc
    11641 gataccatca ttttaaggat ggaaaaggat ggaaactaac taaagataat ccaagttcct
    11701 ttaaaaatcg gtatcgaaaa ttaaagaaac aaggtctttt aagtgtccga aatatctatc
    11761 aaaaattaaa aggagccttt tccttcttta catttgtggc tggaaatcct gtaatctgga
    11821 tagttggagg aatagtcttt cttcttttac ttatgatgag cttcttttta ggattttcat
    11881 ctgctagttt gattcaacaa gatgaatttg aattaacaaa agcttatacc cacctaactt
    11941 gggaagatgc agaacatact cgtacaaatg acaaaggaat tacctattac acaaaagttg
    12001 atgatgtgat gggctatatg aactttaaat tccatgacta tgagttacag aaaccagttc
    12061 atttgtttag ttcagaaact tacaaggatt atctatctgc tttgtggcat gatttaaacg
    12121 atggggaaga tttgaaatcc atgcaagacc tttatgaaac tcctaagtat aaactctcga
    12181 aagacgatca agaggaaata aaggaactaa aagaagaagg tgtgtatgct tccatgcagg
    12241 aattggacaa tccatttgag gggaaaagta acgaagatag tttgaccatg acttatcgtt
    12301 atggatacta tgatttagac ggaaaaccta ctcttcagga gtacattcta ttagaagcga
    12361 aggctcacca aacgattgtc gcaccaatgg atggagttgt atctctagat ggagatgatg
    12421 ttattctcac taacggaaaa ggtgagaatg agagtcgttt aaccttatat tccattcata
    12481 atggccgtgc gattgagggg acaagggtct taacgggtga tgttattggt gaaacaccag
    12541 atgatacagg tttgaaagtt tcctatcaaa agtataagaa caagaaagaa aaattggtat
    12601 atgtcaatcc gcaattttat tttccaaaag tcattcaact tcagacaact atcttacctg
    12661 ccattggtca gtttggtgga gatgagtttg aacgagcaaa acatatttat gattttttga
    12721 aatctcaagg ggcaagtccc caagccattg cggctatttt aggaaattgg tcggtagagt
    12781 cttctatcaa tcctaaacga gctgaagggg attatttatc tcctccagtt ggcgctaccg
    12841 attcctcatg ggatgatgaa acctggttag cgataggagg gccagccatt tatagtggtg
    12901 cttatcctaa tatccttcat agaggtttgg ggttaggtca atggacggat accgcagatg
    12961 gttcaacacg tcatacagct ttgttaaatt atgcacgcac ccaaaataag aaatggtatg
    13021 atttagacct acaacttgat tttatgcttc atggggatag tccttactat caaagttggt
    13081 taaaggattt ctttaaaaat acgggcagtg cagccaatct cgcccaactc ttcctcacct
    13141 attgggaggg aaattctggt gacaaactac tggaaagaca aaccagagca acggaatggt
    13201 attaccaaat tgaaaaaggc ttcagccaaa caaatggagg acaggcaaaa agtgacccgc
    13261 aatcccttga aggtgttcgt ggagacttgt atgaacattc tgttcctggt ggtggagatg
    13321 gtatggccta tgcctatgga caatgtacat ggggtgttgc ggctcgtatg aatcaattag
    13381 gcttaaaatt aaaaggaagc aatggagaga agatttcaat cattaatacc atgggaaatg
    13441 gtcaggactg ggttgcgaca gcttcaagtc ttggtggaga aacgggttcc acaccaagag
    13501 caggtgctat tgtttctttt gtaggaggta cacatggtac accagctatc tacggtcatg
    13561 tggcttttgt agagaaggtc tatgatgatg gttctttcct tgtgtctgaa actaactatg
    13621 gtggcaaccc taactatacc tttagaaaaa tctctcatgc agatagtgcc attagttttg
    13681 cctatactac gaaataaaga agtttacact tgcaatttta gctattttca ggtaaaataa
    13741 atagtgtaga tgagtggtcg gctcatggtc aatctgatag taatcttgga cgtaagggcc
    13801 aagcggtggc ggacaccaga acgaaatccg atagcaatct tggacgtaag ggccaagcgg
    13861 tggcagacac cagaataaac cgactgacta ggtggcttac agactctgta aaccaccttt
    13921 ttttgtgagg aaaatatggc aaattctaga gattatcgta atccaaatta caccgaaaca
    13981 ataaaacttc agcgtttttt tacacagtta caaattgcgg cttctttttt taaagaacac
    14041 tttgttggca aaattatgta ttatgaaacg gaaattgaaa gtgttgaact tcatttttca
    14101 cctactaatt tcatgcacct atgtggtgtt gattaccgaa aaggtgcagg cagtttcttt
    14161 gacgattgtt taaatagaca tgtcataatt gatgagttga aaataaaaaa agatggaacc
    14221 acgatgcaaa agttacaagt tttaggatcc attggggagt tattgggaaa gcatgttcat
    14281 ctaactggtt caggacgtta tttgtatttg gagtttgatt atgctctacg tacaagaaaa
    14341 cagataatag cattaacatt gaaagagacc tctaggaaaa ttgttcctca atctttatta
    14401 gatttgaaga gaaagacagt atttccaaaa ggtcaaaaag
//
LOCUS       DAVKWS010000047        13686 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKWS010000047 DAVKWS010000000
VERSION     DAVKWS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.047"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            2..1138
                     /gene="dnaN"
                     /locus_tag="VW852_001580"
     CDS             2..1138
                     /gene="dnaN"
                     /locus_tag="VW852_001580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV0272697.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1203..1397
                     /locus_tag="VW852_001581"
     CDS             1203..1397
                     /locus_tag="VW852_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV0272698.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1481..2596
                     /gene="ychF"
                     /locus_tag="VW852_001582"
     CDS             1481..2596
                     /gene="ychF"
                     /locus_tag="VW852_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV0272699.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKATQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2667..3236
                     /locus_tag="VW852_001583"
     CDS             2667..3236
                     /locus_tag="VW852_001583"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV0272700.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3237..6746
                     /gene="mfd"
                     /locus_tag="VW852_001584"
     CDS             3237..6746
                     /gene="mfd"
                     /locus_tag="VW852_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV0272701.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEMSQLEPCRIEFFGDEIDGIRSFEVETQLSKENKI
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELVLQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6804..7070
                     /locus_tag="VW852_001585"
     CDS             6804..7070
                     /locus_tag="VW852_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV0272702.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7063..7431
                     /locus_tag="VW852_001586"
     CDS             7063..7431
                     /locus_tag="VW852_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV0272703.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7551..8819
                     /locus_tag="VW852_001587"
     CDS             7551..8819
                     /locus_tag="VW852_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV0272704.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKAQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8816..10093
                     /gene="tilS"
                     /locus_tag="VW852_001588"
     CDS             8816..10093
                     /gene="tilS"
                     /locus_tag="VW852_001588"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV0272705.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10097..10639
                     /gene="hpt"
                     /locus_tag="VW852_001589"
     CDS             10097..10639
                     /gene="hpt"
                     /locus_tag="VW852_001589"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV0272706.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10655..12613
                     /gene="ftsH"
                     /locus_tag="VW852_001590"
     CDS             10655..12613
                     /gene="ftsH"
                     /locus_tag="VW852_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV0272707.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12735..13214
                     /locus_tag="VW852_001591"
     CDS             12735..13214
                     /locus_tag="VW852_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV0272708.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLADDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            13308..13379
                     /locus_tag="VW852_001592"
     tRNA            13308..13379
                     /locus_tag="VW852_001592"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13341..13343,aa:Glu,seq:ttc)
BASE COUNT         4734 a         2056 c         2822 g         4074 t
ORIGIN      
        1 catgattcat ttttcaatta ataaaaattt atttctacaa gcattaaata ctactaagag
       61 agctattagt tctaaaaatg ccattcctat tttatcaaca gtaaaaattg acgtgaccaa
      121 tgaaggtatt actttaattg gttcaaatgg tcaaatttca attgaaaatt ttatttctca
      181 aaaaaatgaa gatgctggtt tgttaattac ttctttaggt tcgatccttc ttgaagcttc
      241 tttctttatc aatgtagtat ctagtttacc tgatgtaact cttgatttta aagaaattga
      301 acaaaatcaa attgttttaa ccagtggcaa atcagaaatt accctaaaag gaaaagatag
      361 cgaacaatat ccacgaatcc aagaaatttc agcaagcact cctttaatac ttgaaacaaa
      421 attactcaag aaaattatta atgaaacagc ctttgctgca agtacacaag agagtcgtcc
      481 gattttaaca ggtgtccact tcgtattgag tcaacacaaa gagttaaaaa cagttgcaac
      541 agactctcat cgcctaagcc agaaaaaatt gactcttgaa aaaaatagtg atgattttga
      601 tgtcgtaatt cctagccgtt ctctacgcga attttcagcg gtatttacag atgatatcga
      661 aactgtagag attttctttg ccaataacca aatcctcttt agaagcgaaa atattagctt
      721 ctatactcgt ctcctagaag gaaactatcc tgatacagat cgcttgattc caacagactt
      781 taacactact attactttta atgtggtaaa cttacgccag tcaatggagc gtgcccgtct
      841 tttatcaagt gcgactcaaa atggtactgt gaaacttgaa attaaggatg gggttgttag
      901 cgcccatgtt cactctccag aagttggtaa agtaaacgaa gaaatcgata ctgatcaggt
      961 tactggtgaa gatttgacca ttagtttcaa cccaacttac ttgattgatt ctcttaaagc
     1021 tttaaatagc gaaaaggtga ccattagctt tatctcagct gttcgtccat ttactcttgt
     1081 gccagcagat actgacgaag acttcatgca gctcattaca ccagttcgta caaattaagt
     1141 gaaagaggtt gagcctggct cgcctctttt atgatataat cgaaaaagaa aaggagagta
     1201 gtatgtatca agttggaaat tttgttgaga tgaaaaaatc acacgcttgt acaatcaagt
     1261 caactggtaa aaaggctaat cgttgggaaa ttacacgtgt aggagcagat atcaaaataa
     1321 aatgcagtaa ttgtgagcat gttgtcatga tggggcgata tgattttgag cgaaaaatga
     1381 ataaaattat tgactgagaa cccttagtta gagggttagc actttatccc tttttgtgtt
     1441 ataatattag ggattgaaat gaaaacggag aatgagaaat atggctttga cagcaggtat
     1501 cgttggtttg ccaaacgttg gtaaatcaac actatttaat gcaattacaa aagcaggagc
     1561 agaggcagca aactacccat ttgcgactat tgatccaaat gttggaatgg tggaagttcc
     1621 agatgaacgc ctacaaaaac taactgaaat gataactcct aaaaagacag ttcccacaac
     1681 atttgaattt acggatattg cagggattgt aaaaggagct tcaaaaggag aagggctagg
     1741 gaataaattc ttggccaata ttcgtgaagt agatgcgatt gttcacgtag ttcgtgcttt
     1801 tgatgatgaa aatgtgatgc gcgagcaagg acgtgaagac gcctttgtag atccacttgc
     1861 agatattgat acaattaatc tggaattaat tcttgctgac ttagaatcag tgaacaaacg
     1921 atatgcgcgt gtagaaaaga tggcacgtac gcaaaaagat aaagaatcag tagcagaatt
     1981 caatgttctt caaaagatta aaccagtcct agaagacggg aaatcagctc gtaccattga
     2041 atttacagat gaggaacaaa aggttgtcaa aggtcttttc cttttgacga ctaaaccagt
     2101 tctttatgta gctaatgtgg acgaggatgt ggtttcagaa cctgactcta tcgactatgt
     2161 caaacaaatt cgtgaatttg cagcgacaga aaatgctgaa gtagtcgtta tttctgcgcg
     2221 tgctgaggaa gaaatttctg aattggatga tgaagataaa aaagagtttc ttgaagccat
     2281 tggtttgaca gaatcaggtg tagataagtt gacgcgtgca gcttaccact tgcttggatt
     2341 gggaacttac ttcacagctg gtgaaaaaga agttcgcgct tggactttca aacgtggtat
     2401 gaaggctact caagcagctg gtattatcca ctcagacttt gaaaaaggct ttattcgtgc
     2461 agtaaccatg tcatatgaag atctagtgaa atacggatct gaaaaggccg taaaagaagc
     2521 tggacgcttg cgtgaagaag gaaaagaata tatcgttcaa gatggcgata tcatggaatt
     2581 ccgctttaat gtctaaaaat taataaatgg tgtcaattag gttggaaaaa aattccaacc
     2641 cttttggctt ttgaaaggaa aaataaatga ccaaattact tgtaggcttg ggaaatccag
     2701 gggataaata ttttgaaaca aaacacaatg ttggttttat gttgattgat caactagcga
     2761 agaaacagaa tgtcactttt acacacgata agatatttca agctgaccta gcatcctttt
     2821 tcctaaatgg agaaaaaatt tatctggtta aaccaacgac ctttatgaat gaaagtggaa
     2881 aagcagttca tgctttatta acttactatg gtttggatat tgacgattta cttatcattt
     2941 acgatgatct tgacatggaa gttgggaaaa ttcgtttaag agcaaaaggc tcagcaggtg
     3001 gtcataatgg tatcaagtct attattcaac atataggaac tcaggtcttt aaccgtgtta
     3061 agattggaat tggaagacct aaaaatggta tgtcagttgt tcatcatgtt ttgagtaagt
     3121 ttgacaggga tgattatatc ggtattttac agtctgttga caaagttgac gattctgtaa
     3181 actactattt acaagagaaa aattttgaga aaacaatgca gaggtataac ggataaatgg
     3241 tgaccttatt agatttattc tcagaaaatg atcagattaa aaaatggcat caaaatttaa
     3301 cagataagaa aagacaacta atacttggtt tatcaacatc tactaaggct cttgcaattg
     3361 caagcagttt agaaaaagaa gataggattg tgttattgac gtcaacttat ggagaagcag
     3421 aaggacttgt tagtgatctt atctctatct tgggtgagga actcgtctat ccatttttgg
     3481 tagatgatgc tcctatggtg gagtttttga tgtcttcaca ggaaaaaatt atttcacggg
     3541 ttgaagcctt gcgttttttg actgattcat ctaagaaagg gattttagtt tgtaatatcg
     3601 cagcaagtcg attgatttta ccgtctccca atgcattcaa agatagtatt gtaaaaatct
     3661 cagttggtga agaatatgat caacacgcgt ttatccatca gttaaaggaa aatggctatc
     3721 gaaaagttac tcaagtacaa actcagggcg aatttagtct tcgaggagat attttagata
     3781 tttttgaaat gtcccagtta gaaccttgtc gaattgagtt ttttggtgat gaaattgatg
     3841 gtatcaggtc atttgaagta gaaacacaat tatcgaaaga aaataagata gaactcacta
     3901 tctttccagc tagtgatatg cttttgagag aaaaggatta tcaacgagga cagtcagctt
     3961 tagaaaaaca aatttcaaaa actttatcac ctattttgaa atcataccta gaagaaattc
     4021 tttcaagttt tcatcaaaag caaagtcatg cagattctcg gaagttttta tctttgtgct
     4081 atgataagac atggactgtc tttgattata ttgaaaaaga tactccaata ttctttgatg
     4141 attatcaaaa attgatgaat cagtatgaag tctttgaaag agaattagcg cagtacttta
     4201 cagaagaatt acagaatagt aaagcatttt ctgatatgca gtatttttct gatattgaac
     4261 aaatctataa aaaacaaagt ccagtgacct ttttctctaa tcttcaaaag ggtttaggaa
     4321 atctcaaatt tgacaaaatt tatcaattca atcaatatcc tatgcaggaa tttttcaatc
     4381 agttttcttt tctaaaagaa gaaattgaac gatataaaaa aatggattac accattattc
     4441 tgcagtctag caattcaatg ggaagtaaaa cattggagga tatgttagag gaatatcaga
     4501 ttaaattgga ttctagagat aagacaagta tctgtaaaga atctgtaaac ttaatagagg
     4561 gtaatctcag acatggtttt cattttgtag atgaaaagat tttattgata actgaacatg
     4621 agatttttca aaagaaatta aagcgtcgtt ttcgaagaca acatgtttca aatgcagaga
     4681 gattaaaaga ttacaatgag cttgaaaaag gggactatgt tgtccatcat atccatggga
     4741 ttggtcaata tctaggaatt gaaaccattg aaatcaaggg aattcatcgc gattatgtca
     4801 gtgtccaata ccaaaatggt gatcaaattt ctatccccgt ggaacagatt catctactgt
     4861 ccaaatatat ttcaagtgat ggtaaagctc caaaactcaa taaattaaat gacggtcatt
     4921 ttaaaaaggc caagcaaaag gttaagaacc aggtagagga tatagctgat gatttaatca
     4981 aactctactc tgaacgtagt cagttgaagg gttttgcttt ctcagctgat gatgatgatc
     5041 aagatgcctt tgatgatgct ttcccttatg ttgaaacgga tgatcaactt cgtagtattg
     5101 aggaaatcaa gagggatatg caggcttctc agccaatgga ccgactttta gttggggatg
     5161 ttggttttgg aaagactgaa gttgctatgc gtgcagcctt taaagcagtc aatgatcaca
     5221 aacaggttgt cattctagtt ccgacgacgg ttttagcgca acagcactat acgaatttta
     5281 aggaacgatt ccaaaatttt gcagttaatg ttgatgtgtt gagtcgcttt agaagtaaaa
     5341 aagagcagac tgcaacactt gaaaaattga aaaacggtca agtcgatatt ttgattggaa
     5401 cacatcgtgt tttgtcaaaa gatgttgtgt ttgctgattt gggcttgatg attattgatg
     5461 aggaacagcg atttggtgtc aagcataagg aaactttgaa agaactgaag aaacaagtgg
     5521 atgtcctaac cttgaccgct acgccaatcc ctcgtaccct ccatatgtct atgctgggaa
     5581 tcagagattt gtctgttatt gaaactccgc cgactaatcg ctatcctgtt cagacctatg
     5641 ttttggaaaa gaatgatagt gtcattcgtg atgctgtctt gcgtgaaatg gagcgtggag
     5701 gtcaagttta ctatctttac aacaaagttg acacaattgt tcagaaggtt tcagaattac
     5761 aggagttgat tccggaggct tcgattggat atgttcatgg tcgaatgagt gaagtccagt
     5821 tggaaaatac tctattagac tttattgagg gacaatacga tatcttggtg acgactacta
     5881 ttattgagac aggggtggac attccaaatg ctaatacttt atttattgaa aatgcggacc
     5941 atatgggctt gtcaacctta tatcagttaa gaggaagagt cggtcgtagt aatcgtattg
     6001 cttatgctta tctcatgtat cgtccagaaa aatcaatcag tgaagtctct gaaaagagat
     6061 tagaagcgat taaaggattt acagaattgg gctctggctt taagattgca atgcgagatc
     6121 tttcgattcg tggagcagga aatcttttag gaaaatccca gtctggtttc attgattctg
     6181 ttggttttga attgtattcg cagttattag aggaagctat tgctaaacga aacggtaatg
     6241 ctaacgctaa cacaagaacc aaagggaatg ctgagttggt tttgcaaatt gatgcctatc
     6301 ttcctgatac ttatatttct gatcaacgac ataagattga aatttacaag aaaattcgtc
     6361 aaattgacaa ccgtgtcaat tatgaagagt tacaagagga gttgatagac cgttttggag
     6421 aatacccaga tgtagtagcc tatcttttag agattggttt ggttaaatca tacttggaca
     6481 aggtctttgt tcaacgtgtg gaaagaaaag ataataaaat tacaattcaa tttgaaaaag
     6541 tcactcaacg actgttttta gctcaagatt attttaaagc tttatccgta acgaacttaa
     6601 aagcaggcat cgctgagaat aagggattaa tggagcttgt atttgatgtc caaaataaga
     6661 aagattatga aattttagaa ggtctgctga tttttggaga aagtttatta gagataaaag
     6721 agtctaagga agaaaattcc atttgatatt tttcttctat aaaatagata aaaatggtac
     6781 aataataaat tgaggtaata aggatgagat tagataaata tttaaaagta tcgcgaatta
     6841 tcaagcgtcg tacagtcgca aaggaagtag cagataaagg tagaatcaag gttaatggaa
     6901 tcttggccaa aagttcaacg gacttgaaag ttaatgacca agttgaaatt cgctttggca
     6961 ataagttgct gcttgtaaaa gtactagaga tgaaagatag tacaaaaaaa gaagatgcag
     7021 caggcatgta tgaaattatc agtgaaacac gggtagaaga aaatgtctaa aaatattgta
     7081 caattgaata attcttttat tcaaaatgaa taccaacgtc gtcgctacct gatgaaagaa
     7141 cgacaaaaac ggaatcgttt tatggggggg gtattgattt tgattatgct attatttatc
     7201 ttgccaactt ttaatttagc gcagagttat cagcaattac tccaaagacg tcagcaatta
     7261 gcagacttgc aaactcagta tcaaactttg agtgatgaaa aggataagga aacagcattt
     7321 gctaccaagt tgaaagatga agattatgct gctaaatata cacgagcgaa gtactattat
     7381 tctaagtcga gggaaaaagt ttatacgatt cctgacttgc ttcaaaggtg ataaaatgga
     7441 aaatttatta gacgtaatag agcaattttt gagtttgtca gatgaaaagc tggaagaatt
     7501 ggctgataaa aatcaattat tgcgtttaca agaagaaaag gaaaggaaga atgcgtaaat
     7561 tcttaattat tttgttgcta ccaagttttt tgaccatttc aaaagtcgtt agcacagaaa
     7621 aagaagtcgt ctatacttcg aaagaaattt attacctttc acaatctgac tttggtattt
     7681 attttagaga aaaattaagt tctcccatgg tttatggaga ggttcctgtt tatgcgaatg
     7741 aagatttagt agtggaatct gggaaattga ctcccaaaac aagttttcaa ataaccgagt
     7801 ggcgcttaaa taaacaagga attccagtat ttaagctatc aaatcatcaa tttatagctg
     7861 cggacaaacg atttttatat gatcaatcag aggtaactcc aacaataaaa aaagtatggt
     7921 tagaatctga ctttaaactg tacaatagtc cttatgattt aaaagaagtg aaatcatcct
     7981 tatcagctta ttcgcaagta tcaatcgata agaccatgtt tgtagaagga agagaatttc
     8041 tacatattga tcaggctgga tgggtagcta aagaatcaac ttctgaagaa gataatcgga
     8101 tgagtaaagc tcaagaaatg ttatctgaaa aatatcagaa agattctttc tctatttatg
     8161 ttaagcaact gactactgga aaagaagctg gtatcaatca agatgaaaag atgtatgcag
     8221 ccagcgtttt gaaactctcc tatctctatt atacgcaaga aaaaataaat gagggtcttt
     8281 atcagttaga tacgactgta aaatacgtat ctgcagtcaa tgattttcca ggttcttata
     8341 aaccagaggg aagtggtagt cttcctaaaa aagaagataa taaagaatat tctttaaagg
     8401 atttaattac gaaagtatca aaagaatctg ataatgtagc tcataatcta ttgggatatt
     8461 acatttcaaa ccaatctgat gccacattca aatccaaaat gtctgccatt atgggagatg
     8521 attgggatcc aaaagaaaaa ttgatttctt ctaagatggc cgggaagttt atggaagcta
     8581 tttataatca aaatggattt gtgctagagt ctttgactaa aacagatttt gatagtcagc
     8641 gaattgccaa aggtgtttct gttaaagtag ctcataaaat tggagatgcg gatgaattta
     8701 agcatgatac gggtgttgtc tatgcagatt ctccatttat tctttctatt ttcactaaga
     8761 attctgatta tgatacgatt tctaagatag ccaaggatgt ttatgaggtt ctaaaatgag
     8821 ggaacaagat tttttaaatc attttctcaa gaagggatat ttcaaaaagc atgctaaggc
     8881 ggttctagct ctttctggtg gattagattc tatgtttcta tttaaggtat tgtctactta
     8941 tcaaaaagag ttagagattg aattgattct ggctcatgtg aatcataagc agagaattga
     9001 atcagattgg gaagaaaagg aattaaggaa gttggctgct gaagcagaac ttcctattta
     9061 tatcagcaat ttttcaggag aattttcaga agcgcgtgca cgaaattttc gttatgattt
     9121 ttttcaagag gtcatgaaaa agacaggtgc gacagcttta gtcactgccc accatgctga
     9181 tgatcaggtg gaaacgattt tgatgcgctt gattcgagga actcgcttgc gctatctatc
     9241 aggaattaag gagaagcaag tagtcggaga gatagaaatc attcgtccct tcttgcattt
     9301 tcagaaaaaa gactttccat caatttttca ctttgaagat acatcaaatc aggagaatca
     9361 ttattttcga aatcgtattc gaaactctta cttaccagaa ttggaaaaag aaaatcctcg
     9421 atttagggat gcaatcttag gcatcggcaa tgaaatttta gattatgatt tggcaatagc
     9481 tgaattatct aacaatattg atgtggaaaa tttacagcag ttattttctt actctgagtc
     9541 tacacaaaga gttttacttc aaacttatct gaatcgtttt ccagatttga atcttacaaa
     9601 agctcagttt gctgaagttc agcagatttt aaaatctaaa agccagtatc gtcatccgat
     9661 taaaaatggc tatgaattga taaaagagta ccaacagttt cagatttgta aaatcagtcc
     9721 gcaggctgat gaaaaggaag atgaacttgt gttacactat caaaatcagg tagcttatca
     9781 aggatattta ttttcttttg gacttccatt agaaggtgaa ttaattcaac aaatacctgt
     9841 ttcacgtgaa acatccatac acattcgtca tcgaaaaaca ggagatgttt tgattcaaaa
     9901 tgggcataga aaaaaactca gacgtttatt tattgatttg aaaatcccta tggaaaagag
     9961 aaactctgct cttattattg agcaatttgg tgaaattgtc tcaattttgg gaattgcgac
    10021 caataatttg agtaaaaaaa cgaaaaatga tataatgaac actgtacttt atatagaaaa
    10081 aatagatagg taaaaaatgt tagaaaacga tattaaaaaa gtcctcgttt cacacgatga
    10141 aattacagaa gcagctaaaa aactaggtgc tcaattaact aaagactatg caggaaaaaa
    10201 tccaatctta gttgggattt taaaaggatc tattcctttt atggctgaat tggtcaaaca
    10261 tattgataca catattgaaa tggacttcat gatggtttct agctaccatg gtggaacagc
    10321 aagtagtggt gttatcaata ttaaacaaga tgtgactcaa gatatcaaag gaagacatgt
    10381 tctatttgta gaagatatca ttgatacagg tcaaactttg aagaatttgc gagatatgtt
    10441 taaagaaaga gaagcagctt ctgttaaaat tgcaaccttg ttggataaac cagaaggacg
    10501 tgttgtagaa attgaggcag actatacctg ctttactatc ccaaatgagt ttgtagtagg
    10561 ttatggttta gactacaaag aaaattatcg taatcttcct tatattggag tattgaaaga
    10621 ggaagtgtat tcaaattaga aagaataatc tttaatgaaa aaacaaaata atggtttaat
    10681 taaaaatcct tttctatggt tattatttat ctttttcctt gtgacaggat tccagtattt
    10741 ctattctggg aataactcag gaggaagtca gcaaatcaac tatactgagt tggtacaaga
    10801 aattaccgat ggtaatgtaa aagaattaac ttaccaacca aatggtagtg ttatcgaagt
    10861 ttctggtgtc tataaaaatc ctaaaacaag taaagaagga acaggtattc agtttttcac
    10921 gccatctgtt actaaggtag agaaatttac cagcactatt cttcctgcag atactaccgt
    10981 atcagaattg caaaaacttg ctactgacca taaagcagaa gtaactgtta agcatgaaag
    11041 ttcaagtggt atatggatta atctactcgt atccattgtg ccatttggaa ttctattctt
    11101 cttcctattc tctatgatgg gaaatatggg aggaggcaat ggccgtaatc caatgagttt
    11161 tggacgtagt aaggctaaag cagcaaataa agaagatatt aaagtaagat tttcagatgt
    11221 tgctggagct gaggaagaaa aacaagaact agttgaagtt gttgagttct taaaagatcc
    11281 aaaacgattc acaaaacttg gagcccgtat tccagcaggt gttcttttgg agggacctcc
    11341 ggggacaggt aagactttgc ttgctaaggc agtcgctgga gaagcaggtg ttccattctt
    11401 tagtatctca ggttctgact ttgtagaaat gtttgtcgga gttggagcta gtcgtgttcg
    11461 ctctcttttt gaggatgcca aaaaagcagc accagctatc atctttatcg atgaaattga
    11521 tgctgttgga cgtcaacgtg gagtcggtct cggcggaggt aatgacgaac gtgaacaaac
    11581 cttgaaccaa cttttgattg agatggatgg ttttgaggga aatgaaggga ttatcgtcat
    11641 cgctgcgaca aaccgttcag atgtacttga tcctgccctt ttgcgtccag gacgttttga
    11701 tagaaaagta ttggttggcc gtcctgatgt taaaggtcgt gaagcaatct tgaaagttca
    11761 cgctaagaac aagcctttag cagaagatgt tgatttgaaa ttagtggctc aacaaactcc
    11821 aggctttgtt ggtgctgatt tagagaatgt cttgaatgaa gcagctttag ttgctgctcg
    11881 tcgcaataaa tcgataattg atgcttcaga tattgatgaa gcagaagata gagttattgc
    11941 tggaccttct aagaaagata agacagtttc acaaaaagaa cgagaattgg ttgcttacca
    12001 tgaggcagga cataccattg ttggtctagt cttgtcgaat gctcgcgttg tccataaggt
    12061 tacaattgta ccacgcggcc gtgcaggcgg atacatgatt gcacttccta aagaggatca
    12121 aatgcttcta tctaaagaag atatgaaaga gcaattggct ggcttaatgg gtggacgtgt
    12181 agctgaagaa attatcttta atgtccaaac tacaggagct tcaaacgact ttgaacaagc
    12241 gacacaaatg gcacgtgcaa tggttacaga gtacggtatg agtgaaaaac ttggcccagt
    12301 acaatatgaa ggaaaccatg ctatgcttgg tgcacagagt cctcaaaaat caatttcaga
    12361 acaaacagct tatgaaattg atgaagaggt tcgttcatta ttaaatgagg cacgaaataa
    12421 agctgctgaa attattcagt caaatcgtga aactcacaag ttaattgcag aagcattatt
    12481 gaaatacgaa acattggata gtacacaaat taaagctctt tacgaaacag gaaagatgcc
    12541 tgaagcagta gaagaggaat ctcatgcact atcctatgat gaagtaaagt caaaaatgaa
    12601 tgacgaaaaa taaccctgag agaggctgga gtctctcttt tttgtgcagt ttaggagcta
    12661 aagggaacag aatggagaaa atggaacaaa tgtgttttct aatctgttag actgtatcta
    12721 gaaaggggaa aattatgatt aaagaattgt atgaagaagt ccaagggact gtgtataagt
    12781 gtagaaatga atattacctt catttatggg aattgtcgga ttgggaccaa gaaggcatgc
    12841 tctgcttaca tgaattgatt agtagagaag aaggactggc agacgatatt ccacgtttaa
    12901 ggaaatattt caaaaccaag tttcgaaatc gaattttaga ctatatccgt aagcaggaaa
    12961 gtcagaagcg tagatacgat aaagaaccct atgaagaagt gggagagatc agtcatcgta
    13021 taagtgaggg aggtctctgg ctagatgatt attatctctt tcatgaaaca ctaagagatt
    13081 atagaaacaa acaaagtaaa gagaaacaag aagaactaga acgcgtctta agcaatgaac
    13141 gatttcgagg gcgtcaaaga gtattaagag acttacgcat tgtgtttaag gagtttacta
    13201 tccgtaccca ctagtaagtc atgcaaaaaa aatgaaaaaa attagaaaaa gtagttgaca
    13261 aagtttgaaa agactgtata atagtaagag ttgaaaataa caactcaggt ccgttggtca
    13321 aggggttaag acaccgcctt ttcacggcgg taacacgggt tcgaatcccg tacggactat
    13381 ggtatgttgc ggttggaaca cttgatgaaa aaaagtttaa aaaagcttaa aaatcttcaa
    13441 aaaagtgttg acaagtgaaa gtggctgtga tatactaata tagttgtcgc ttgagagaag
    13501 caagtgacaa agacctttga aaactgaaca agacgaacca atgtgcaggg cgctacaaca
    13561 taagttgtag tactgaacaa tgaaaaaaca ataaatctgt cagtgacaga aatgagtgag
    13621 aactcaaact tttaatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta
    13681 atacat
//
LOCUS       DAVKWS010000048        13619 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKWS010000048 DAVKWS010000000
VERSION     DAVKWS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.048"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..273
                     /locus_tag="VW852_001593"
     CDS             <1..273
                     /locus_tag="VW852_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0272709.1"
                     /translation="RIAILLNLSTLYLYNQDKNMCKQICYTLLEDAKNKKSYDRLAIC
                     YVRIGICRDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQPKKL"
     gene            complement(563..1033)
                     /locus_tag="VW852_001594"
     CDS             complement(563..1033)
                     /locus_tag="VW852_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV0272710.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(999..1805)
                     /locus_tag="VW852_001595"
     CDS             complement(999..1805)
                     /locus_tag="VW852_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0272711.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(2012..2653)
                     /locus_tag="VW852_001596"
     CDS             complement(2012..2653)
                     /locus_tag="VW852_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272712.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGALVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(2650..4758)
                     /locus_tag="VW852_001597"
     CDS             complement(2650..4758)
                     /locus_tag="VW852_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0272713.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(4810..5097)
                     /locus_tag="VW852_001598"
     CDS             complement(4810..5097)
                     /locus_tag="VW852_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0272714.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWAIASLTKIPPTGMEYFYGFE"
     gene            5522..5842
                     /locus_tag="VW852_001599"
     CDS             5522..5842
                     /locus_tag="VW852_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0272715.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(5894..6481)
                     /locus_tag="VW852_001600"
     CDS             complement(5894..6481)
                     /locus_tag="VW852_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0272716.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            6722..7393
                     /gene="sdaAB"
                     /locus_tag="VW852_001601"
     CDS             6722..7393
                     /gene="sdaAB"
                     /locus_tag="VW852_001601"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV0272717.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7402..8274
                     /gene="sdaAA"
                     /locus_tag="VW852_001602"
     CDS             7402..8274
                     /gene="sdaAA"
                     /locus_tag="VW852_001602"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV0272718.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            8296..8928
                     /locus_tag="VW852_001603"
     CDS             8296..8928
                     /locus_tag="VW852_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0272719.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            8978..9055
                     /locus_tag="VW852_001604"
                     /pseudo
     CDS             8978..9055
                     /locus_tag="VW852_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9323..11173)
                     /locus_tag="VW852_001605"
     CDS             complement(9323..11173)
                     /locus_tag="VW852_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0272720.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(11215..12372)
                     /locus_tag="VW852_001606"
     CDS             complement(11215..12372)
                     /locus_tag="VW852_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0272721.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            12430..13596
                     /locus_tag="VW852_001607"
     CDS             12430..13596
                     /locus_tag="VW852_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV0272722.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
BASE COUNT         4087 a         3013 c         2405 g         4114 t
ORIGIN      
        1 cgaatagcaa tcctgctcaa cctttccaca ctctatctat acaatcaaga taaaaacatg
       61 tgtaagcaaa tctgctacac tttactggag gacgccaaga ataagaaaag ctacgatagg
      121 cttgctatct gctatgttcg tattgggatt tgtagggata atgctaaact tatccaaaaa
      181 ggtttctcac tcctagagct gaccgaggaa acttctatgc tgtctcatct caaaaaagaa
      241 gtagagaccc attatcaacc aaagaaatta taaaaaaagt cgagggattt cctcgactct
      301 ttacatatta tttgttcgtt agttcttaat atcagccatt gttaaaccag aagtttttag
      361 ctctgccaag ccacatagtg tgtttacttc tatgtgtcca tgtcaccatg aatcgccgaa
      421 cgatttaacg agaaagtatg acttttacgt ttatcccaac tcaattatga catttttttc
      481 aaaagtcaat atatctcact ttttcaacga caagaaagag gctgataatc taccaacctc
      541 ttattctgaa cccatcactc catcactttt tagcttcatt cgctttctta gcgactgcaa
      601 tctggtattc gacttggtca ttccccttac ctgtacaacc atgagcaatt gtagtcgctc
      661 ctgtctgatg cgctatttca accaattttt tagaaataag agggcggctc aaggcagata
      721 ccaaggggta cttttgttca tagtaggcat gtgactggag agctactagc acataatctg
      781 tagcaaattc gtccttaaca tcaatgacat aagattcgac tgccccaacc ttaagagcct
      841 tatcatggat gaaatctaag tctttccctt cacccacatc catacaaact gcaacaacat
      901 catagtcttt cttcagccag gtaatagcaa ctgatgtgtc taaaccgcca gaataggcca
      961 aaattacttt ttctttactc atgttctttt ccttctttct atttttcaat ggacgcttta
     1021 aaggcatcct caatgagttt gtctaattcc ccagactcct tcaacttttg aatggtttta
     1081 tcgactgcct ctttcaattc cttgctatct tttttcatgg ctaccgcata ggaatcatct
     1141 tgctcttttt caaaattgag gtctgcgatt gctaaatcag gattattttc cacaaatccc
     1201 ttggcaacgg gttcttcaaa aataacggca tccacttgcc ctgattttaa atctgtgatt
     1261 aaattcccat ttttaggcag agatacgagg gaagaatttt gtagcaaatc tttcgccatc
     1321 gtctcttgaa tcgaaccttt ctgcgctccg acctttttct gcgccaaatc gttgacagac
     1381 tgataagtgg ctaaatcaga ttttttgaca atgagtttat tttttgcagt atagtaggga
     1441 gttgaaaaat caaacacctt gctccgttca tctgtcttag aaacacctga tatggcaagg
     1501 tcagcttttc ctgattgaac gctagccagt acattgtcaa aactcatagg agatagttcc
     1561 aattcgacac ctagttctgt tgcgatagcc ttggctaatt cgatatctga acccacaatc
     1621 tgatttttcc catcaaccac tttttgatat tcaaatggag caaaatctgg attgagggct
     1681 acaaccaatt ttcctttgga cttgatggct tcaattccag ccgattgatt gttactacaa
     1741 gcaaatagta gggggagcat cactaaacca aacatcgtca tcaacacctt cttcatctta
     1801 ttcataacaa agcctccttt atgtttattc ttttttattg tataaataat actcctatac
     1861 tcatcctttg tcaagcctaa attgaaaaaa ttttcaattt gaaacaaaaa aaacctagaa
     1921 atactacata aaatgtcatt cctaggcgta tttatgctat ttttctctgg aaaacatgag
     1981 tattcagcca gtcaaaagga gctgaatgaa ctcattttcc ctcgcccaat tcaatgacac
     2041 gatgacattg ttgggctaca taagcatcgt gggtcacaat aatgactgtt ttcccttctc
     2101 gattcatctc taagagaaac ttcaagacca aatctctatt ttcaggatcc agcgaacctg
     2161 tcggttcatc ggctaaaatc agctggctgg gttttaagat ggctctagca actgcaattc
     2221 gttgttgttc gcccccagac aactcggaga ccctttgatg caaagtagct gacaaaccta
     2281 ctctctctaa aatctcttcc acctttttga gcttgtcttt cttaggcaat ttcacatatt
     2341 tcagcgccag catgagattg tactcgaccg tttcatcatc aatcagggca aaattttgaa
     2401 acagataaga gatatgttca cggattattg tttgcgactt agcagaatta accgctagat
     2461 ttgtctgacc aaaaatctca taccgtccgc tataatcacc atctatcaaa cccaataaat
     2521 ttaacaaggt cgacttgcca ctgccacttt tgccaacaat ggctaccaaa gccccctgat
     2581 caatcctgag agataagtta tccaaaatca cttttccccc aatggttttg gtaatatttt
     2641 tcaactcaat cataagatgc cccctttcaa taactctact agacttcttt tctccatcct
     2701 agaagctaag cctagcacaa atagtatatc cagacatgta aaacctgcaa acagtagaag
     2761 tggtaagaac gcatgagcaa agaaaatcaa gactagaaga gggaaactat agcccagcaa
     2821 gagcagaacg aggagaggac ggtagcgatc gaccagtttc caccccataa acttcttggt
     2881 aatgatatcc ctgcgcttca ataagaaagt tgttactagt aagaagtagg aaatcatcat
     2941 gctaaggaga ccaaacaaag caaagagtag gttaaaattc cgaacagcat ctctataaga
     3001 atccactttc tcttgttgaa tggcttgaat agatgaaaat tttaaataat ttccatctga
     3061 caatttctca actaactctg taatctcttt ttgatgttga accgtatttt caattttaat
     3121 cggattattt aagccagttg ttgacaggga ggctttctca tcccacatca tatcagaatc
     3181 attgaccaag ctaataattg gattggagag attttccttt cgcttatcac tatatgggaa
     3241 aaatgaccaa tctccttcat aataggcaat ctcgacatcc atctcctcta tcgttcgttt
     3301 ttgctgctct tcatacttca tcgaatgaaa ggcaattaac ttccccaaga gctgattttt
     3361 atcttcttca cctttcgtac ttgctggcat caaaataact tttttaatac cggtatctgg
     3421 tagcttgaat cccttgctct ttagaaaatt gcgattggca tagtaaacat ccaccgtatc
     3481 tgttaactga tattgctgaa tctgttctga ttggacaaaa ttttttacag gaagactgct
     3541 actctgcaca tagcccgcct gcgttttttc taccaaatcc tgataaaatc gatagaaata
     3601 atctgtagat ttccctgacc ctgctagctc ttcttgccac agattatcat tgagtttgaa
     3661 ggtttctaag gtcaggtaat taccttgact tacccactgt tgctgataag caagttcttt
     3721 gttttcttgt tctaaacttc tgcccacccc aatcagtaag gccgtcagta aaatagttgt
     3781 ccctattttc atcacataat tgaagataag accaaatttg aaagatgaaa aacctttcag
     3841 cagagagctg attgtcattt tttggattaa gaggtaagtc aaccaactga taaagagata
     3901 aagctgcaac agcaaaaaat gagacaacca cagcatagga aacaaatctt ttggcttata
     3961 atcaagcaag aaaaacacgc ctagattgat cacaagagcc ccacctagga ggaggtaaag
     4021 gttgcctttt acaacatcag ctaaaacagc cctatcttga aaaccaagta atttttgtac
     4081 cccaactctt ttcatctcca tcatcggttg atacactgtc actaacacaa gaagcaaaat
     4141 agccaagaca aaaacaatgg cagataaaag caaatctcga tttatgactt ccactgcact
     4201 tttgtaggtc ggctctagca aggtagcctg gtctatcttg aaaaaatcgc tccatttctg
     4261 tacaatccta tccttgtcca tctcttgtgt agaagttatc gtatagcgac catttaaact
     4321 acgagatgta tccttgatat aggtttgaaa agtcataagc tgaataggtt tggcttttag
     4381 aaaggtcgga atcgtaccaa gtttattgga aatttcttta ttactataga ctccttcacc
     4441 atctgtggta aaatcaagag aagaaatccc aaactcttgg taggggaagg tatctttatc
     4501 aaaaacacca gacttgacca cctcatcacc actgtctgtt ttgatgatgg agactttata
     4561 ctcctttgat acatcctcaa aaaatcgaag aacagacgct gcaggttcgt taatatcttt
     4621 caaatacaaa tccaaagaat ctacagtctt tcccatttct tgaatccgaa ccacttctcg
     4681 tgtatagttt acattaaaaa caaaaaaagt ggtacacaga agcaggagta acatacagaa
     4741 attactgatt tttttcataa aagtgcctcc actacagttt tccctctcca aataggagag
     4801 ggaagaattt tactcaaatc catagaaata ttccattcct gttggcggaa tcttggtgag
     4861 ggaagctata gcccatgcct caggttttgc ataatcctta gaggttctac ccccatgtct
     4921 aacagttgct gtatgatatc gagtagaatg taagtaatca gaatatccaa aagttcctgt
     4981 ccaacctact ccatagttcc attgaccacc ctcaacccat actgctaaag catttgtagt
     5041 agcaagtata ccacatacca ttaaagatgc aacaccaatt ttaactaatt ttctcatgat
     5101 ttatttctcc tattatcgag agctgttccg taaaaacagc tatagtaatt tatcaacaag
     5161 tatcgtagtc cattggactc accttccttc tatctcattt cattttttaa caacgaactt
     5221 actcttaacc gggagaatgg tgcgcttctg tatcaggtcc ttgaacaacc agagtagtat
     5281 taagcacact ctaactatta ctcaagaatc agtccaataa acaatatgga tagcgctttc
     5341 aaatttttaa actcctatcc catcctttta tctatataat aagtgaaaat ataataactg
     5401 tcaagtaact gaagtgaatt ttataaaaaa attacaagcc aaatttgtaa agtttacact
     5461 aagccgctag ggcaatcgtc tatcagaatg tccgtttatt tgtcaataat ccgagaaaat
     5521 cttgcaacgc ttagaagtct ataaaaacta tcaacattta tatgacttgc gaatagcaat
     5581 cctgctcaac ctttccacac tctatctata caatcaagat aaaaacatgt gtaagcaaat
     5641 ctgctacact ttactggagg acgccaagaa taagaaaagc tacgataggc tggctatctg
     5701 ctatgtccct attgggattt gtagggatga tgcgaaactt atccaaaaag gtttctcact
     5761 cctagagcta accgaggaaa cttctatgct gtctcatctc aaaaaagaag tagagatcta
     5821 ttaccaagcg aaggaaagat aaaaaagccg agggatttcc tcgactcttt acatattatt
     5881 tgttcgttag ttcttaatac cagccattgt taagccagaa gtttttagca gctgtccatg
     5941 aaccgtaacg tcctgcaacg taggcatctg ctacacgttc ttggttttca gctgagtagt
     6001 caccgttcag gtatgaatct gttaattggt aacgtccgat ataacgtcca tttgtagctg
     6061 tatagctacc acctgattct ttttgagcga tccattcttt ggcttctgct tcagatccgc
     6121 ttacagttga aacaactgtt tcacttacta ctggagtttc tgccactgga gctgaaacag
     6181 tttcatcttg agcggctggt gccgcataag tagctggcgc tggtgttgca acaggcgcta
     6241 cagggccatc gataaccaac tcttgatcaa cataaatcaa atgaatgtta tcaatgtggt
     6301 tgttttctgc caatttttcc actgttgtgt tgtgagtttc agcgatttct gaaagtgtat
     6361 caccttcttt aacagtgtaa gttgatgatt cttgagcaga tacaaatgat ggagcaaata
     6421 ctgcaaacaa ggcagctact cctgcaagag ttgctttaat ctttttagtt attgatttca
     6481 tattcgaaaa ttctccttct ttctatagtt ctatcatacc ctttaaatat taccgtttct
     6541 tgacactttt atgtagaaat attacaaaat tattttttat ttccctaaat aaggtgtttt
     6601 tgtcataaaa gatactattt ttatgctatt tgaggtatta gaaaggtttt catccacaat
     6661 taaagccaag cccctcattc tttgatataa tagagataag aatttttata aggggaaact
     6721 gatgaaatca cttcgttttc aatctgtctt tgatatcatc ggaccagtta tgattggccc
     6781 atctagtagc cataccgctg gtgctgttcg tattgggaag attgtctctt ccatttttga
     6841 tgatactccg acagaagttg aattccaact ttttaactca tttgccaaga cctatcgtgg
     6901 tcacggaaca gacctagccc ttgttgcagg tattttagga atggatacag atgatcctga
     6961 aattccaaat agtctggaaa ttgcccacaa gcgtggtatc aagattgtct ggaccattca
     7021 gaaagacagc aatgcccctc accctaacac cactaaaatt actgttaaaa atgcccacaa
     7081 gaccatcagc gtgactggta tttctatcgg tggaggaaat attcaggtaa ccgaactcaa
     7141 tggttttgcc gtctctctca atatgaatac accgactatc atcatcgttc atcaagatat
     7201 tccaggtatg attgcccttg ttacagaggc gctttcccgc tatggtatca atatcgccca
     7261 gatgaatgtc actcgtgaaa aagctggtga aaaagccatt atgattatcg aagttgacag
     7321 tcgcaactgt gatgaggcta tcgaagaaat tcgaaaaatc cctcatctcc acaatgtcaa
     7381 tttctttaaa taggaggaag catgttttat tctatcaaag aattggtcga gcaagcagat
     7441 ctggactttc aaggaaatgt cgcagaactc atgattacaa cagagtttga attgaccggt
     7501 cgcgaacgtg aagaagtctt ccttctcatg gaacgcaatc tggaagttat gaaagcctct
     7561 gtccaacttg gcctcaatga aaataaatct cgtagtggcc tgacaggtgg agatgctgcc
     7621 aaattggatc actacatcga aaacggaaaa actctgtcag attatacgat tctctcggct
     7681 gcccgaaatg ccatcgcagt caatgaacac aatgccaaaa tgggcttggt ctgtgccact
     7741 ccaaccgctg gaagtgctgg ctgtctgcca tccgttctca ctgctgctat tgaaaaatta
     7801 gacctcagcc acgagcaaca attggatttc ctctttgctg ctggtgcctt tggactagtc
     7861 atcgcaaaca atgcctccat ctcaggtgct gagggtgggt gtcaagctga agttggttca
     7921 gcctctgcta tgagtgctgc cgccttgact ctggctgcag gtggaacacc ttatcaggcc
     7981 agtcaagcta ttgcctttgt cattaaaaat atgctaggcc tcatctgtga ccctgttgca
     8041 ggtttggtcg aagttccctg tgtcaaacgt aatgccatgg gagctagctt tgctttcatc
     8101 gcagcagaca tggccttggc aggtatcgaa tctaaaatcc ctgtggatga agtgatcgat
     8161 gccatgtacc aagtaggagc aagcatgcca actgcctttc gtgaaacagc tgaaggtgga
     8221 ctcgctacca cccctactgg tcgtcgcctc caaaaagaaa ttttcggaga ataagcttat
     8281 caaataggag aaaccatgac ctctatcaca gcgatttttt tcgatttgga tggaaccctc
     8341 gttgatagtt ctatcgggat tcacaatgcc tttacctata cctttaaaga gctgggggtg
     8401 cctagccctg atgccaaaac tattcgtggt tttatgggac cacctctcga aagtagtttt
     8461 gcgacctgcc tgtccaaaga ccaaatttct gaagccgtgc agatatatcg ttcttactat
     8521 aaggcaaaag gcatctatga agctcaactc tttcctcaga ttatagactt gcttgaggag
     8581 ttatcgagca gttatccact ctacatcacc acgacaaagg atacatctac tgctcaagac
     8641 atggcaaaaa acttggaaat ccatcatttc tttgatggca tttatggttc tagccctgaa
     8701 gcaccccata aggcagatgt cattcaccaa gccttgcaga cacatcaact agcaccagaa
     8761 caagccatca tcatcggaga taccaagttt gatatgctgg gagctcgaga aacaggtatt
     8821 cagaaattgg ccatcacttg gggatttgga gagcaagcag atctactaaa ctatcaacct
     8881 gattatatcg ctcacaaacc cttagaagtt ttggcgtatt ttcaatagca tagactgggt
     8941 aacaattcca tttcagaaga aaatgaagta atactcaatg aaaatcaaag agcaaactag
     9001 gaaactagct gcgggctgct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt
     9061 cagctcaaaa cattgtttta aggttgtgga tggaactgac gaagtcagct caaaacactg
     9121 ttttaaggtt gtggatggaa ctgacgaagt cagctctaaa cactgttttg aggttgtgga
     9181 tagaactgac gaagtcagta accataccta cggcaaggcg acgttgacgt ggtttgaaga
     9241 gattttcgaa gagtataagt ctctacataa taaaacgcat attaccaagg ttgtgtaact
     9301 tgatactatg cgtttttctt ttttattcaa tgtgggttgt ttgattagca aaggcgataa
     9361 tagcttgctt caactcatct ccactgagag tgcggaactc tccaatcttt tgattgatgg
     9421 attctaaagg catgacatta accttaccaa cgaaaatctt atccataact tgattgtcaa
     9481 cgagttcggt tgataccaag agttcttcgt agagtttttc acctgggcgg attccaactt
     9541 caacgattgg aatttcactt tcagtgtggc cacttagaag caccatcttc ttggccaagt
     9601 cataaatctt gactggtttg cccatatcaa ggataaagac ttccccatct ttggcataag
     9661 caccagcatg gataaccaga cggctagctt ctggaatggt cataaagtaa cgggtcatgc
     9721 ggaagtctgt caccgttaca ggcccacctt cagcaatctg acgttcaaag actggaatga
     9781 cactaccacg gctaccaaga acattcccaa aacgaactgc acagtaggtt gattggctac
     9841 gttggttaaa gccagtgaca atcaactccg ccacgcgctt ggttgctccc ataacatttg
     9901 gtggattaac tgccttatct gtcgaaatca taaccatctt aggcaccttg gcttcatcaa
     9961 cggctctagc aacattgtaa gttccacgga tattgttttt gaaggcttct tttggattgc
    10021 gctccatcat aggaacatgc ttgtgggctg ccgcatgata aacaatagca ggtttgtact
    10081 gctcaaagac ttgcaacaaa cgatcatagt cttgaatgtc cgcaatcaca ggtacataat
    10141 caatcccttg gaacttacga atcaattcat gataaacaag gtagattgag ttttccccat
    10201 gaccgagcaa gacaatgcgt tcaggattga agcgactaac ttgacgacag atttcagaac
    10261 cgattgaacc tccagctcct gtgactaaga tggtcttacc tgtcagttct gcacccagac
    10321 gcgattcgtc aagacggatt tcctgacgac ccaaaaggtc cgtaatatca attttttgga
    10381 agccagtacc tgcttggtga aggccctgaa caacagtttc aaccttaggc atcttgtaac
    10441 atttgacacc cagcttatta cacatctgca agatacgctc atattctgac ggatccagcg
    10501 acggaatcgc aacgatgaca cgctcgattt gatggcgttt ggctaattca ggcagattgt
    10561 cataagagcc caaaacagga ataccaccaa gtttttgacc ctttttctta gaatccttat
    10621 ccaaaatacc gaccagttct aattcactgg ttggatgttg gtaactatcc ataaagagag
    10681 ccccaccatc accggcacca atcaagaagg tccgacggtg ttctccatca ccactacctt
    10741 ttttgcgtct ggagtagatt aactgccaag taatccgtgg caataaaatc aagaaggtac
    10801 tcaacaagat aaagagaatg atgaaacgga tggagaagag tggcaagaag gcataacaga
    10861 tactatatga caagacactg ctagcagtca caccaaaaaa gattttcatg aaatccgtaa
    10921 tcttgctgta acgactaatg ctcgcgttca acccccaaaa accaatcatc aattgataga
    10981 acaggaaggc caaactcgta tagataatgt agtcaacagg tgctggatta atcagcccat
    11041 aaaataaaat ataagataca atgatggaaa ccaccatact gaaaatatca aatattcccc
    11101 agaaaacctg cttttgttgt ttatttaaaa tttccaccag atcaatcaca taatctgtta
    11161 gttttttatt catagaaatc tactcctcct taaaagagtt ttactatatt gttattatag
    11221 catatttttg tttatttggc ttattttatc caaataaatt attgttctct tgtactcctt
    11281 cttccattta aatcctctaa ttaaacctac aatcgtccca aagccataag caaagtgaat
    11341 ggaaaataaa agaaagggca tcacaattag aaatccattt ttatgtttta ataaagtcag
    11401 caaagtgagt agtgacaaaa gtagaaaata ggcacctaat agtaaagtta tgaatacgaa
    11461 tgtgatcggt aacaatgcta gactaaacac aagactcaaa acaaataaac aaggaacata
    11521 gtgaaataat gataaacact taaactgaac atgacttgtc aagccaatcc acaaaccatt
    11581 taaatacttt tgatgcagca ttttcttgaa tgttggtcga atatactgat aagatagaat
    11641 acttgggcta tagcggattt tataaccata ttctcgaatt ctataatgaa tatcattatc
    11701 ttcagttcgg ccaagttgct catttactaa accaaccttc tggaaaacct ctcgtttata
    11761 cattccatga aaaatagaag aaacatatct atcctcagaa ctatttcgat aattggcaat
    11821 gctactgcca aacatatttt cctcaacaag atgcaaggtc tctgcccatt ttccttttcc
    11881 ttcgacaatc gtcggtctag gccccccaca gacaaattca ccttgttgaa taatagccac
    11941 attgttcatt acaaaagtct cagtaacttt tgaatgagca tcaattttta aaataaggtc
    12001 ccctacagaa tgtttaactc ccaggttaaa accactagct tgatttttct taggattgtt
    12061 atacaatcta attgagttaa actctgtatc ttcctttata aattgctgaa tgatagctgt
    12121 ggtcccatct gtggacatag catttataaa tagaatttca atattctctt taggataggt
    12181 ttgatttttt aagtcttcaa ttagaccagg cagatatttt tcttcattgt atgcactgat
    12241 tacaatggat acgagaagac tttcaacatt ttgagtcact actactctcc tgaatttttc
    12301 cttccaccat tctatcataa ttttcaagac tttcccattt ttatcttgaa agccagaaac
    12361 cttacttgac attatagtga aaaagcctta aaataagctg ttattaaaat cagctagaag
    12421 aggtattata tgaaaaagca atcactcttt tttgttccag gtattatcct gattggtgtt
    12481 tccttgcgaa ctccttttac tgttttaccc attattttgg gaaatatttc gcaaggtctg
    12541 gaggtagaag ttagttcgct tggtgtcttg accagcctgc ctctccttat gtttaccctc
    12601 ttttcaccat tttctaccca actggctcag aaaatcggct tggagcatct cttcacctac
    12661 agcctcttct tcttgaccat cggctcactt attcgactaa tcaatctgcc cctgctctat
    12721 ctaggaacct tgatggttgg ggcaagtgtc gcagtcatca atgtcctgct tcctagtctt
    12781 atccaagcca atcaaccaaa gaaaattggt tttctgacca ccttatatgt aacgtctatg
    12841 gggattgcaa cggctctggc ttcctatcta gctgtgccca ttacacaagc cagttcttgg
    12901 aaaggactta tccttctcct cacgttactc tgtctagcaa cttttttggt ctggctccca
    12961 aatcaccgct ataatcatag actagctcca caaaccaaac aaaaaagtca aataaaggtc
    13021 atgcgtaata aacaggtttg ggcaattatt atcttttcag gttttcaatc cttgatcttt
    13081 tacaccgtca tgacctggtt acctaccatg tctatccatg caggtctatc cagtcacgaa
    13141 gctggcttgc tgacttctat cttatctctg attagcattc ctttttcaat gaccatccca
    13201 agcctgacaa ccagtttatc tactcgcaac cgtcagctca tgctcactct ggtttcacta
    13261 gctggtgtgg tcggcatttc catgctcttt ttcccaatca ataatttcat ttactggctt
    13321 gccatccatc tcctcatcgg aaccgcaacc agtgccctct tcccttatct catggtcaac
    13381 ttttcactca agacaagcgc ccctgaaaag acagcccaat tgtccggcct atctcaaaca
    13441 ggaggctata tcctagcagc ctttgggcca accctctttg gttacagttt tgacctgttc
    13501 cactcttggg taccatctgt agctgccctc ttgctcatcg atatcctgat gactgtggcc
    13561 ctctttacag tggacagagc ggataagatc ctttaaactt ctagttttag ctagaagtt
//
LOCUS       DAVKWS010000049        13409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKWS010000049 DAVKWS010000000
VERSION     DAVKWS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.049"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            63..389
                     /locus_tag="VW852_001608"
     CDS             63..389
                     /locus_tag="VW852_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0272723.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            376..969
                     /locus_tag="VW852_001609"
     CDS             376..969
                     /locus_tag="VW852_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV0272724.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKAAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            962..1873
                     /locus_tag="VW852_001610"
     CDS             962..1873
                     /locus_tag="VW852_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV0272725.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            1936..3000
                     /locus_tag="VW852_001611"
     CDS             1936..3000
                     /locus_tag="VW852_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272726.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(3072..3932)
                     /locus_tag="VW852_001612"
     CDS             complement(3072..3932)
                     /locus_tag="VW852_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV0272727.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWRIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLESLPSAFYHQLDANQLEVQPILG
                     QDFKTLAQEK"
     gene            complement(4054..5040)
                     /locus_tag="VW852_001613"
     CDS             complement(4054..5040)
                     /locus_tag="VW852_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV0272728.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPAIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(5312..6787)
                     /locus_tag="VW852_001614"
     CDS             complement(5312..6787)
                     /locus_tag="VW852_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV0272729.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(6997..7920)
                     /locus_tag="VW852_001615"
     CDS             complement(6997..7920)
                     /locus_tag="VW852_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0272730.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(7934..8863)
                     /locus_tag="VW852_001616"
     CDS             complement(7934..8863)
                     /locus_tag="VW852_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0272731.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(9182..9403)
                     /locus_tag="VW852_001617"
     CDS             complement(9182..9403)
                     /locus_tag="VW852_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272732.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(9357..9617)
                     /locus_tag="VW852_001618"
     CDS             complement(9357..9617)
                     /locus_tag="VW852_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272733.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            9697..9921
                     /locus_tag="VW852_001619"
                     /pseudo
     CDS             9697..9921
                     /locus_tag="VW852_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10025..10180)
                     /locus_tag="VW852_001620"
                     /pseudo
     CDS             complement(10025..10180)
                     /locus_tag="VW852_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(10357..10968)
                     /gene="rpsD"
                     /locus_tag="VW852_001621"
     CDS             complement(10357..10968)
                     /gene="rpsD"
                     /locus_tag="VW852_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV0272734.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(11163..12215)
                     /locus_tag="VW852_001622"
     CDS             complement(11163..12215)
                     /locus_tag="VW852_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0272735.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(12212..12910)
                     /locus_tag="VW852_001623"
     CDS             complement(12212..12910)
                     /locus_tag="VW852_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272736.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(13077..>13409)
                     /locus_tag="VW852_001624"
     CDS             complement(13077..>13409)
                     /locus_tag="VW852_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0272737.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT         3966 a         2840 c         2557 g         4046 t
ORIGIN      
        1 tacaatgtat gtgtagaaaa attatatata aaaatcttac acattagaaa aggaggtttc
       61 ccatgtactt tccaacatcc tctgccttga ttgaatttct catcttggcc gtactggagc
      121 agggtgattc ttatggttat gagattagcc aaaccattaa gctaatcgct aatatcaaag
      181 aatccacact ctatcccatt ctcaaaaaat tggaaggcaa tagctttctg acaacctatt
      241 ctagagagtt ccaaggtcgc atgcgcaaat actactcctt gacaaacggt ggtatagagc
      301 agctcttgac cctaaaagat gaatgggcac tctatacaga caccatcaat ggcatcatag
      361 aagggagtat ccgccatgac aagaactgaa tacctgactc agctagaact ctatctcaag
      421 aaactacctg aagctgaccg tatcgaagcc atggactatt tcagagagct ctttgacgat
      481 gctggagtcg aaggagaaga agaactcatc gctagtttgg gaactcccaa agcagcggcc
      541 cacgaagttc tatccaatct tctcgataaa aaaatcaatg aagcacccgc tcaaaaaaat
      601 aaccgacaaa ttttacatat cgccttgtta gccctccttg cagcacctat cggcattcct
      661 ctgggaatcg ccatcctcgt gaccctgttc gcaatccttg tagccgcttt gactgtcatt
      721 ctggctttct ttgcagtttc catactgggt atcatcggcg gattcctatt tttagttgaa
      781 agtttcacta tcctcgccca agccaaatca gcctttatct tgatttttgg ttctggttta
      841 ctggctatcg gtgcttcttc gctagtttta cttggcattt cctatgtagc tcgcttcttc
      901 ggtctactca ttgttcgtct ggtacaattt gttcttaaaa aaggaaagag aggtaatcag
      961 catgcgtaaa tggacaaaag gatttctcat ctttggtgtg gtgactaccg ttatcggctt
     1021 tatcctgctt tttgtaggta tccaatctga cgggattaag agcctacttt ccatgtccaa
     1081 agaacctgtc tatgatagcc gtacggaaaa gctaaccttt ggcaaggaag tcgaaaacct
     1141 agaaattact ctccaccaac acgcgctcac catcacagac tctttcgatg atcaaatcca
     1201 catttcttac catccatctc tttctgctca ccatgatctt atcaccaatc agaacgatag
     1261 aactctgagt ctcactgata agaaactgtc tgaaactccg tttctctctt ctggaattgg
     1321 tgggattctt catatcgcaa gtagctactc tagtcgtttt gaagaagtta ttctccagct
     1381 accaaaaggg agaactctaa aagggatcaa catctcagcc aatcgcggac aaaccaccat
     1441 cataaatgct agccttgaaa atgcaaccct caatacaaac agctatatcc tccgaattga
     1501 aggaagtcgt atcaaaaaca gtaaactcac aacgcccaat atcgttaata tctttgatac
     1561 agttcttaca gatagtcagc tagagtcaac agagaatcac ttccacgctg aaaatatcca
     1621 agtccatggc aaggttgaac tgactgccaa agattatctc agaatcatcc tagaccagaa
     1681 agaaagccaa cgaattaact gggacatctc aagcaactat ggttctatct tccaattcac
     1741 aagagaaaag cctgaatcaa gaggtacgga attaagcaac ccttacaaaa ctgaaaaaac
     1801 cgatgtcaag gatcaactca ttgcgagatc tgatgataat attgatctaa tatccacacc
     1861 aagcagacgt tgacaaaaac gcttacatct gctactatgg aagcatattt atctaaccaa
     1921 aaaggaggtt ctaccatgaa acaagaatgg tttgaaagta atgattttgt aaaaacaaca
     1981 agcaagaaca agcctgaaga gcaagctcaa gaggttgcag acaaggctga agaaacgata
     2041 gccgatttcg atacaccaat tggaaaaaat actcaagtag aggaggaagt ctctcaagct
     2101 gaagtcgaac tggaaagcca gcaagaagag aaaattgaaa ctcctgaagt cggtgaagcg
     2161 agaacagaaa tagaagaaaa gaaggcatct aattctactg aagaagagtc agacctttct
     2221 aaagaaacag aaaaagtcac tatagctgaa gagagccaag aagctcttcc tcagcaaaaa
     2281 gcaaccacga aagagccact tcttatcagt aaatctttag aaagtcctta tatccccgac
     2341 caagctccaa aatctaggga taaatggaaa gagcaagtgc ttgatttttg gtcttggcta
     2401 gtggaagcga tcaaatctcc tacaagtaag tttgaaacaa gtatcacaca cagttacaca
     2461 gcctttctct tgctcattct gttttctgca tcttcctttt tctttagtat ctatcacatc
     2521 aaacatgctt actatggaca tatagcaagc attaacagtc gcttccctga gcagctagct
     2581 cctttaactc ttttttctat catctctatc ctagtagcga caacactctt cttcttttca
     2641 ttcctcttgg gtagtttcgt tgtgagacga tttatccacc aggaaaagga ctggacgcta
     2701 gacaaggttc tccaacaata tagtcaactc ttggcaattc caatcttcct cactgctatt
     2761 gctagtttct ttgctttctt tgatagccta cgatttacag ccctcttgtg tgtgattagc
     2821 attggaatca ttctgcttgc cagtctccat atcattacaa gaccaagtca agcaagtgaa
     2881 accgactcct tctatcaatt attcttgtct gtccttgtga acggagttat tatcctcctc
     2941 ttctttgtag ctgaagtggc actgattgga gattatcttc gtatcttggc ctttctttaa
     3001 aattcaatcc tgtcatcctt ggcaggattt tttctatgtc aaaaaagcaa gtcgattgac
     3061 ctgcttctgc tttacttttc ttgtgctaat gttttaaaat cttgtcctag gatgggttgt
     3121 acttctaatt gattggcgtc tagttggtgg taaaatgctg atggtagtga ctctaggaat
     3181 tttgcatagt ccaagcgggc gatggcttca tagtcttctg gtttagagcc atctcctgtg
     3241 atttgaacca agtcgatctc ccactggact tccttatcca ccaattgctc aatataggct
     3301 gcaggaacaa acggttctct cggtaaaact gtcttgactc cttgagctag atggtaaacg
     3361 ccatgcactt ttccttctcc atcctgatag aatgaaactc tcgcaaagta ggcatctgtc
     3421 tcttgaaccg cacgcacacg cgcttcaaac tgagccaagc catctgcctc taaaaagctt
     3481 ttcaagttct cacgactaaa gaaaatagga ttaaaaattc cttggcaaat cgtaaaacga
     3541 gctacagggt ctgctagata ggcttgaata cttgcttgga aataagcttc aaaatcaaaa
     3601 ccatctagcc cttcaatatc agtccctgta taagcaagca aagcatctaa aaatgacttg
     3661 ataatccgcc agtctgtctc tgttagtagg tcaggaagat cgacacggta agccttttga
     3721 ccatcagcat aactgacctt aaagagaaat tgtgattgcc ccactatcgc acactcaata
     3781 tactcgagac gattaagagg ctgacgaaga tagactacat catagctatg tgactccaaa
     3841 ccgtctagca aggccaagat cgacttggca ttcaaaatct cttgttctcc taaaatgctc
     3901 tgtttatttg gaataaaaaa tgtttttgcc ataactggac tcctttgaaa tcgtttacat
     3961 acagtatacc ttattttcct gaaagataca aaaacaaact ttagattttt gagtacaaag
     4021 catagaaaaa cagcccctga ggactgtttg atcttataca gtagctgcct gatccaagct
     4081 ttcaccgata gcggctaggc gctcaatcac ttcagcttgt gtcaattcat tttttgaaac
     4141 atagcggtta cgtgggtgaa cacggcactc gtgtgagcat ccacgaaggt acttgtcttc
     4201 attttcttct gatgtcaaga tacgacggtt acagaaggga tttccacagt tgacataacg
     4261 ttcacatggt gttccatcaa accagtcttt ccctacgatg gttgggttga catggttgac
     4321 atcaacggca atacgctcgt caaagacgta cattttccca tcccaaagct caccttgaac
     4381 ttctgggtct ttaccgtaag ttgcgattcc tccgtgcaat tggccgacat ctttgtagcc
     4441 ttcacggacc atccagcctg agaatttctc acagcgaacg ccacctgtac agtaaaccac
     4501 gacacgcttg tccatgaatt tttccttgtt atcacggacc cattgtggta actcacggaa
     4561 gttgcgaata gctgggcgaa tagctccacg gaaatgtcct aggtcgtact cataatcgtt
     4621 acgtgtgtca aggacaacgg tatctttatc aagaagcgct tctttgaact cttttggaga
     4681 caagtaagca cctgttgttt caagtgggtt gatgtcattg tcaaagtcgt tgtcttccaa
     4741 accaaggtgg acaatttctt tcttgtagcg aacaaacatc ttcttgaagg cttgttcact
     4801 ttcttcgtca atcttgaacc aaagttcttc cattcctgga aggctgtgaa cgtagtccat
     4861 gtatttttga gttgtttcat agtcacctga aactgttccg ttaattccct cgtcagcgac
     4921 taggatacgg cctttaaggc cgattgattt acagaaagcc aagtggtctg cagcaaattg
     4981 ctctgcattt tcaattggag tataaaggta gtaaagtaag acacgaatat cttttgccat
     5041 aagatttgtt ctcttttcta ttcttaaatt ttctaaattt tttatttatc catactagta
     5101 tacctgcttg agaaaacgaa tgcaatttat ttgactggaa attgtagagg gttctcgaaa
     5161 tttcctgaat ggttaaaata aggacaagag aaaacatgga tatctatatc cttgtgccaa
     5221 aaaaaccact gccctcccca gaccaacctg aggaaagcag tgattcttat tttaggagtt
     5281 aggaatgaat acacgaaatc aatttagctg attatttttt gtttttcaag aattcatcgt
     5341 attgtttttg catttcgttc aatacttttt cgtaggcacc ttcagatttc aatttttcca
     5401 tcaattctgg aatagcttta tctgggtcta cagtaccagt gttgatagct gtatcaaatt
     5461 gttgcattgt gttagcaata gctgagattt cagatttcac attgtcagta ttgaagataa
     5521 atccaagcgc tggagattct ttagcttctg ccaattcttt cttagaattt tcgatttgtt
     5581 ggtctgtaac gttttcgttg atgtaaagga tccagttgtt accagtgttc catccaccca
     5641 tgtgagtgtt tcctttgtag ccatcaagaa cgcgaacacg gttttcttta ccttcaattt
     5701 tttcccagtt cttgccttct ggaccgtaaa caagaccgtt caagagttct ggattcgtat
     5761 tcaagaggtt caagatttcc attgattttt ctttgttctt agagttgttt gagatgacaa
     5821 agttagcaac ttgtgttgtt tggtttttct tgatgaagtt agtaattggt ttgatttgga
     5881 tatctttgtt ggcaacacgt gaaagcaagc tgttaccgta gtcagctggt cctactgttt
     5941 cttcacgaac gaaccaagta tcttgttgaa ggtcaaagga agtatcgctt gttgcgacgt
     6001 cttttggaat gtagccagct tcatagaatt tgtgaagagt cttcaagtgt tctttgaaac
     6061 gaggcacttc gtaacggttt acaactttag tagtatcgcc ttcaaggtcg ataacgaatg
     6121 gaagaccgtt tgctactggg tagtcaaagt tatcagatgg gatgaaaact ttaccaatag
     6181 caaatggtac tacgtctgga gctttttctt taatttgttt caagactggc tcaagagttt
     6241 cgtaagaagt aacacctgaa atatcgatac catatttagc aaggagagtt ccgttgaagg
     6301 caaagttttg agatgacgca acgttggctg caactggaac agcgtaaatc ttaccattta
     6361 cagtattacc cttgatgtaa gctgggtcaa gtgctttgta aaggtcttta ccttcttttt
     6421 tgtacaattc tgtcaagtca gcgtaagcac ctttttgagc atttacaata tagttatctg
     6481 caaaggcaat atcatagttt tcaccagatg atgtgataac tgacattttc ttaccatagt
     6541 caccccagcc aaggtattgg atatccaatt tggcaccaac tttttcttca atgattttgt
     6601 tggcatttgc taacaattca tccaagttgt ctggtttgtc accgatttgg tacattttga
     6661 taacaggttt gtcacctgaa tcagcagctt ttttactgtt acctgtcaag ttgccacaag
     6721 cagcaagacc tgcagccaaa gcgactacac tagcagatgc aaaagcatat tttttccagt
     6781 ttttcatgat aaaaactcct ttttttattt ttaaacttat aaacaatgta atgatcttat
     6841 actcaatgaa aatcaaagag caaactagaa aactagccgc aggctgtact tgagtacggc
     6901 aaggcgacgt tgacgcggtt tgaatttgat tttcgaagag tattaacttc acacaaggga
     6961 agttgggaac tgagaaatgt tctttttcaa taagcactat tctttcacac caccgatagt
     7021 caaacctttt acaaagtagc gttggaagaa tggatataaa atcgcgattg gaagggttgc
     7081 aaccacaacc atggccatac gacctgtttc tttcggtaga gcaactccca gttgaccagt
     7141 caagccgacc gctttggcaa tgtagtccat attttgttgg atttgcatga gcaaatattg
     7201 caatggatac aagttatcac tcttgatgta aagaagggcg ttgaaccagt cattccagaa
     7261 accaagagct gtcaaaagcg tgatggttgc gatacctggt agtgacaatg gcaaacagat
     7321 ttggaagaaa atccgggcct cactggcacc atcgatacga gccgattcga gaatggcttc
     7381 tggaatggtc ttcttgaaga aggaacgcat caagatgatg ttaaaggggg agagaagcat
     7441 tggcacaatc aaggcccaaa cagtgtcacc aagctgaagt acacgggtca ccatgatata
     7501 acctggtacc aaaccagcgt tgaacaacat actgagaagg acgaagatgg taaaaaatct
     7561 gcgatactta aaggttgtcc gtgaaatagc gtaggcatag gttgttgtga taaagacatt
     7621 tgtcaatgtc ccaactacgg ttacaaagac agagatgaag agggcttgta ggattttatc
     7681 cttaaactgt gccaaaaact caaaaccgtc taagccaaat tgggatggga agaagctata
     7741 gccgtattgg aggaggcttt tctcgtctgt cactgaaata atgataacga atacaaaagg
     7801 taggatacaa gagagggcaa tcaaacccga aatgatactg aagaagatat ctgctttctt
     7861 actgaaggag tgaatgccga cattatcaat tttttctttt ttaattttct tttttgccat
     7921 attctcctcc tttctagaac aaagctgagt ttggatcgac tcgtcttgca agcaagtttg
     7981 ataggataac cagaatcaaa ccaacaacgg attggtaaag accggctgct gcagccatac
     8041 caatatctgc tgtctgagtc aaaccattaa agacatatac gtccaaaacg ttggttacat
     8101 tgtaaagctg accagcattg tgtgggattt gatagaagag accgaagtct gcgcggaaga
     8161 tatttccgac tgcaaggatg gtcaatacag ttacaagcgg agtcaactga ggaatggtta
     8221 cgttgcgaat acgttgccac ttgctagctc cgtccactgt cgctgcttcg tagtaggttg
     8281 gatcaattcc catgatcgtc gcatagtaca tgacactgct atatccaaag cctttccaaa
     8341 tacctaggaa aagtaggaga tagggccaga tgcccaggtc agcgtagaaa ttgacttctt
     8401 tgagaccaag actttccaat agatgattga acaccccttt atcaatattt aggaaggcat
     8461 ctgtaaagaa actgatgata acccaagaca agaagtaagg gaacaacata gaagtttgga
     8521 aaatcttcac cattctctta gaacggagct cgctgaggat aatggcaatc cctacagata
     8581 caactaaacc tagaaagata aagccaagat tgtagaggac agtatttcgt gtgataataa
     8641 aggcgtctct tgaactaaat aagaatctga aattatcgag tccgacccat ttactattta
     8701 tgatactatc tatgaaacca ttactggtca tgtggtagtc tttgaaggca accacgttcc
     8761 caaatactgg aatgtaaaag aatagaatca accagagtgc ccctggcaaa accatcaaga
     8821 gaaagatcca gttgtctctc aatgtttttg aaaacttttt cataatttcc tcccttttta
     8881 ttttgatatc catctaaaaa ttctttttta gacttttgat aacgattaca ttattagtat
     8941 actcctattt gcaggttagg ttaaactcct aattatagaa aaaactccac aaattatgta
     9001 gcagatttaa aactttatca ccactatcaa acaaatgtcc taaatcaatt gtttatttta
     9061 tctctattag cccagtgatg gcgtcactct gttataagca tccaacaacg gggtatactg
     9121 aaaaatctcc agactaggga actcagcgat agttcctaat ctggagattt ttaatatgtt
     9181 attaggcgtt tgctttcaac ttagcaataa cctctttaag attatcaatc acctctgctt
     9241 cagtatgctc agagcctttt tcatctgcca agaacaaaac tgctttttga agttcttttt
     9301 gagagttttc aaggacatcc ttatctactg tttcaaggtt tgagtcttta agaagtttac
     9361 ttaattcctt ggctaatttc ttgagtttga tttgcagact catcttctcc tgctgtttct
     9421 ttgcccgctg tttgtcctcc atccttagtt gctgactggc tttccttaat ggactctagg
     9481 gaagcaatgg catctttgac tgtttgcaag atatcacgta aaccttgctc tgtcaaacta
     9541 tcatctgcaa aagctttctt agcctctgcc aaaaccagac gtgctgaatc tgtggtagga
     9601 ttcgatacac ctgtcaatga tctcaaaaga ttttctaagg tttgagtctg cttactaata
     9661 ctagactaaa atcaaaaagc attatataac agtgatatga aatcaactaa agaagaaatc
     9721 caaaccatca aaacactttt aaaagactct cgtacagcta aatatcataa acgcctttaa
     9781 atcgttctat agtaaaatga aacaagaaca ggacaaatcg atttctaaca atgttttaga
     9841 agtagaggtg tactattcta gtttcaatct attatatttc gtctgatggg caaatcttat
     9901 aaagagatta tagagctttt atagtgattt gaaataagat gtgaacaact ctatcaggaa
     9961 agtcaaatta atttatagaa atattttagc agccaaggtg tactgttata gattcaatac
    10021 actatacttg gtggtttagc tctcctgttt tctcttttag ctttaaccag ccataaatgg
    10081 tattacgtga gatttggaaa acgtgtgatg cttctgttat actatctgtt cactcacaat
    10141 aagagagaac ttttttacga aaatctattg aatattccat aaaagattat accacattgt
    10201 gtactatttt tggttcattt tactatatca ctttggacta tatcggtcta gttatgtcta
    10261 aacgattata gctggatact aagagaaatc aaaaaagcac taagggagaa cgtcccaaag
    10321 tgcttaatat caaagcctat cttattcaat aaaatattac aacattttgt tgtagaattc
    10381 aacgacaagt gcttcgttga tttctgggtt gatttcgtcg cgttctggca agcgagtcaa
    10441 tgaaccttcc aatttttcag cgtcgaatga tacgaatgct ggacgtccaa gagtagcttc
    10501 tactgcttca aggattgctg gaactttcaa tgatttttca cgaactgaga tcacttgacc
    10561 tggagttacg cggtatgatg ggatatcaac gcgtttcccg tcaacaagga tgtgaccgtg
    10621 gtttacgaat tgacgagctt gacgacgagt agtcgcgaga ccaagacggt aaacaacgtt
    10681 atccaaacga cgttccaaaa gaagcataaa gttgaaacct aggattccgc ctttgatttt
    10741 tgtagcttgt acgaacaagt tacggaattg tttttcacct acaccgtaag tgaaacgaag
    10801 tttttgtttt tcagccaatt gcaaaccgta ttctgacaat ttagaacggt tgtttggtcc
    10861 gtgttgtcct ggtacgtagt tacgacgtgc caattcttta cctgtacctg taagtgaaag
    10921 gccaaggcga cgagcttgtt tccaagatgg tcctgtataa cgtgacatat gaatgtcctc
    10981 ctgatataaa taatatttcg gtggaaatag tcacttagaa agccctgatt cgtgcagatg
    11041 cccttcgcct aaacagccaa ggttacttgt cataagacac ctgttgacga gcttcatgct
    11101 ttcctgctga tatttcacac aaaatctatt ctaccatgaa tagctggatt tgtaaagggg
    11161 ttttaggctt tattttcact accagagagg ttgagaacga gggtaaaggt acttcctaga
    11221 ccgtactggc tgctgactgt gatttcccca cccaattgat gggccaattc acgcgcaatc
    11281 gcaagtccta atccatgacc acctgtcttc atgttacgcg aagtttcgac acgataaagg
    11341 cgtttgaaaa tattttccaa atcctctggg gcaataccct gcccttcatc ggtcacactg
    11401 attgaaagct ggtccttctc cagcttagcc accacttcca gcttggttcc tggagcagaa
    11461 tatttaaaag cgttatcgac cagattcacc aagatacgag aaagcttagc ataatctccc
    11521 tcaatccggg cagactctgg gattacctgc aagtggacat ctcttctctc ctgctcaatc
    11581 aaaaactgaa attcactcat gcactcaatt aagagcttgt ccagaaaaat actgtcttta
    11641 ctggtagttt ccacctgatt tctagctgtg tttagggtca aaaaattcaa ctcctcaacc
    11701 agtttattga gcctctccgt ctggcgtcca atggttgcta gataatgagc ttgctccgac
    11761 tccttgataa tcccatccaa aatcccttct accgtcgctt ggatcgaagt gataggagtc
    11821 ttaatatcat gcgacaactg agcaatcatc aagccctttt ctcgttcgct ttcttccaag
    11881 gaatcaaagc ttacctgcaa atcatgggac atctcattaa aagtttgccc taattgctga
    11941 aattctacag gaccttgaac ctccaaattt gaaggaaaat ccttggccgc tacccgcttg
    12001 gcatgctcct tgagtttgcc caacgacgta aagactggca ataggagaaa gagactaatc
    12061 ccagcaccga caaggctggc aacgatggtc atcccaagca aaaagtaaat ctcgcctttc
    12121 gcaatcagca ttttttgaac agcccaaaaa acaaccaaaa tggttaagag ggttgaaata
    12181 atatatccaa ccaaaatata actttttagt ttcatgtttg tcctctcggt ttctctatct
    12241 tatatcccaa cccccaaact gtcttaatag tgggcgtttg gtcactacta tattttgcca
    12301 gctcctgtcg aagagcatgg atatgcacat tcaaggtatt ggtgtcatcc acgtagtctt
    12361 ctttccagat cttttcatag aggtctgtct tggagaaaac tcgctctgga ttgctagcta
    12421 aaatccacag caattcaaat gatttaacag ttaaatccag catttcttct cctatttgaa
    12481 cttcatgact actatgattc atttttagat tgccaaggga aatcagctct gtttcgcccc
    12541 cacgatgaag gcggcgcaaa atattgtgga cacgcaaaac cagctcacgt gggctaaaag
    12601 gcttagcaat aaaatcatct gctcccaagc tcaggccgta aatcttgtcc tgttcactgg
    12661 tcttagcagt aataaatagg aaaggctgct ctggtgataa gtattgaacc tcactgatta
    12721 aatcataacc atccatccga ggcatcatga catctgtgat aatcaaatca atcggttttt
    12781 tcttgaacag ctctagcgct tccagtccat catgggctac caagacctga taacctgcct
    12841 gaattaagta tctctgatga atatctgtga tttctacctc gtcgtcaacg agtaaaattg
    12901 tctttcccat ctgtctctcc tttgataaaa acagtgctat actgctttaa gtataacact
    12961 atttttattg ttttacgatg attttaatct gctattgaaa gaaagtcctg attgctgatt
    13021 gagttgttaa actaatctta gtcagcccac tatccatcat ttatttcctt agctgattag
    13081 ttttcttctt ttttgttttt caaacctaga ccaccgagta aacctgcaag cgcaagccca
    13141 aggaaaccaa tacttgccat tgatgtttga gtctcaccag tatttggtag catagcttta
    13201 tcctctgaca tcatcgtatc agacatcttg ttagcagaag cagccatgtt ttcacctgcc
    13261 atcgtgttgg tagaacttgt catggtgtca gcaggcatgc tatctgtagt acctgtagca
    13321 tgattgtgat tcatcggagt cacgccagaa ccagagttag aaggtgataa tgaaccattt
    13381 gctgtgtctg atgtttcttt aacatttat
//
LOCUS       DAVKWS010000050        13203 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKWS010000050 DAVKWS010000000
VERSION     DAVKWS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.050"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            188..634
                     /gene="rplM"
                     /locus_tag="VW852_001625"
     CDS             188..634
                     /gene="rplM"
                     /locus_tag="VW852_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV0272738.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            653..1045
                     /gene="rpsI"
                     /locus_tag="VW852_001626"
     CDS             653..1045
                     /gene="rpsI"
                     /locus_tag="VW852_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV0272739.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(1306..1728)
                     /locus_tag="VW852_001627"
     CDS             complement(1306..1728)
                     /locus_tag="VW852_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0272740.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     TISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            1875..2051
                     /locus_tag="VW852_001628"
     CDS             1875..2051
                     /locus_tag="VW852_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272741.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            2452..4665
                     /locus_tag="VW852_001629"
     CDS             2452..4665
                     /locus_tag="VW852_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV0272742.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSKTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAIPLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNKNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPMYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTI
                     PVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4887..5363)
                     /locus_tag="VW852_001630"
     CDS             complement(4887..5363)
                     /locus_tag="VW852_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV0272743.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5586..8789
                     /locus_tag="VW852_001631"
     CDS             5586..8789
                     /locus_tag="VW852_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV0272744.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WLHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPEIQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKGINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9142..9527
                     /gene="tnpA"
                     /locus_tag="VW852_001632"
                     /pseudo
     CDS             9142..9527
                     /gene="tnpA"
                     /locus_tag="VW852_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9906..10535)
                     /locus_tag="VW852_001633"
     CDS             complement(9906..10535)
                     /locus_tag="VW852_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV0272745.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(10545..11546)
                     /locus_tag="VW852_001634"
     CDS             complement(10545..11546)
                     /locus_tag="VW852_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV0272746.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISALEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKETYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11577..12218)
                     /locus_tag="VW852_001635"
     CDS             complement(11577..12218)
                     /locus_tag="VW852_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV0272747.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12237..13052)
                     /locus_tag="VW852_001636"
     CDS             complement(12237..13052)
                     /locus_tag="VW852_001636"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV0272748.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4162 a         2313 c         2800 g         3928 t
ORIGIN      
        1 tcagttactt tgagaagtta ccgaaaaata tttttcatat ctattgactt ttaggggtaa
       61 aatttggtat gatagtaggc ggtattgttt accccatttg aaaggccccg gaaccttcca
      121 aatacttttc gatgggaagg aacacccatc accgtaaaca aaaatcgaac tatatatagg
      181 agaaatcatg aacaaaacaa catttatggc taaaccaggc caagttgaac gtaaatggta
      241 cgtagttgac gcaactgatg taccacttgg acgtctttct gcagtagttg ctagcgtact
      301 tcgcggaaaa aacaaaccaa catttacacc acacactgat acaggtgact tcgtgattgt
      361 tatcaatgct gaaaaagtta aattgactgg taaaaaagca actgataaaa tctactacac
      421 tcactcaaac cacccaggtg gattgaaaca aatctctgca ggtgaacttc gttctaaaaa
      481 tgcagtacgt ttgattgaga aatcagttaa aggtatgctt ccacacaata cacttggacg
      541 cgctcaaggt atgaagttga aagtatttgt tggagctgag cacactcacg ctgcacaaca
      601 accagaagtt cttgacattt caggacttat ctaaggaaag gaacaataaa gtatgtcaca
      661 agcacaatat gcaggtactg gacgtcgtaa aaacgctgtt gcacgcgttc gccttgttcc
      721 aggaactggt aaaatcactg ttaacaaaaa agatgttgaa gagtacatcc cacacgctga
      781 ccttcgtctt gtcatcaacc aaccattcgc agttacttca actgtaggtt catacgacgt
      841 tttcgttaac gttgtaggtg gtggatacgc tggtcaatca ggagctatcc gtcacggtat
      901 cgctcgtgcc cttcttcaag tagacccaga cttccgcgat tcattgaaac gcgcaggact
      961 tcttacacgt gactcacgta aagttgaacg taagaaacca ggtcttaaga aagctcgtaa
     1021 agcatcacaa tttagtaaac gttaattcga aagaattact atacttatac agagcacctt
     1081 tcggggtgtt cttttttata ctttcttact aaattggtgc aattgacaca gttgttgcga
     1141 ctttagtcgc ttacaaatgt ggctgcaacc tgacaaggtc agttgcctca aaacgttaat
     1201 caatacaatt atatcaacgt ttcaaagcac tcaagagttt acctcatggg tgcttttttt
     1261 atgttttttg aaaaagttca cctcaaagtt taccttattt tcaccttatt gttttagaga
     1321 ctgtaaggca aattcaccga cggccatagg gactacatta gaagtattga cataaatctg
     1381 tgttgtacct gtgtcgctat gtattagagc ttcagaaatg gtttctaagc tcattcctgc
     1441 ttgtttagcg agcgttgctc cggtatgacg tagtttgtgt ggcgtagcgt gtgtaagctc
     1501 tgggtgtcgt tttctgatag ttttcatctt attattgaga tagtcagaat gtaaaggttt
     1561 attgatattg cctttggtat ctatgtaagt aaagatgaat tgttctggat tactgatgat
     1621 accaaacttt gccagttcat atttttgttg ttttttccat gaagtcagaa gttgaagtaa
     1681 gtcattagaa atagagaaaa tgggaaatgt ttccacaata aaacgcatat tatcagatgg
     1741 ttaatgcgtt ttatatttgg gtttgaggtt tataaaatag gataagcgtg ctgaaaactt
     1801 agatatgaca aacaaaataa aatttttaaa agctacagag aaacatggta taatgtatct
     1861 aaaggaggta gcatatgtcc ttagatatag ataaagaaaa gatgacaatc atgggaatag
     1921 cttttgaaaa ccgatccgtt tttaagagtg tttggtatgc tttaagtaca aatatgattg
     1981 aaggatggcg acctacggtt agtgatgttg aaaaattacg agatgaagct cttgctctgg
     2041 ggatgactta atgaagcgca actgttatga atcttatgac tatgttgatt cggataattt
     2101 atataccgat ccgggttctt cagttctaag aaataagcag gaaggaaaaa agcacgcgag
     2161 cttgaatatc gtatgattgc tagtaagagt ttgaaattat ttatcaatcc tatcttagtt
     2221 aatagtttaa aagtagtttg tttcacaact gcataagctt agaaaagttg aaaagtgata
     2281 actctttaat aaactcttgg tttgttaaaa gatattttgt ccactacatt ttttcaattg
     2341 actattattt gtcatagata taacagatag aacattgcat tcagaataat gaaaaacgca
     2401 tcaaaaatgg tgcgttctgt ttttatgcta ccataaaagg agggaaacaa attgaaaatt
     2461 tttaagggag agttttatcg catctctgta ttaacagaca agctagtaag gttagaatac
     2521 tctaaaactg gcagttttga ggatagaact acacaactta tctataatag agattttggc
     2581 caagtttcgt tagattatat cgagacatca aacgtactag atattatgac ggactatttt
     2641 catctgcact ttaataaagg agaatttaac gccgaaaatt tatttataga attaaaagga
     2701 aattttgccg tatatggtag tcgctggtat tttggtgaat cgattgaaac gttaaaagga
     2761 acagctcgga ctctggataa ggcagatgga gcaatcccat tagaagatgg aattattagt
     2821 cgaaatggta tagccttatt ggatgattct caaggattta tttgggatga acagtctggt
     2881 tatattgaga gagaaaatca aattgacctc tatttctttg cctatgggca tgattataga
     2941 ggagcaatca gagactttta ccatttgact ggttcaacac ccttgttacc aagatatgct
     3001 ttagggaatt ggtggagtag atattggcct tatacgtcgg atgaatactt ggatttaata
     3061 gacagatttg aaacagagaa aattccatta tctatcggtg tgttagatat ggattggcat
     3121 ataactgaca ttccagctcg ttttggaagt ggctggacag gatatagttg gaataaaaac
     3181 ttaataccta atccagaaca gttattgcaa caacttcatg atagaaagtt aaaactctct
     3241 ttaaatgtcc atcctgctga tgggatacgg gcttatgaag aagcttatcc tcaagtcgcc
     3301 aaacgattag ggttaaatgt agaactagaa gaacctgcca tttttgattt ttttaatccc
     3361 tcttttaggg aagcctactt taaagatgtt cattatgagc tagaaaagca gggagtagat
     3421 ttttggtgga ttgactggca acaagggact caaggcatat tggatccact atggctttta
     3481 aaccattatc attatcagga tagttgtaaa aatgcagaag gtggtttgat tttatcaaga
     3541 tatgcaggtc ctggtagcca ccgctaccct gttggttttt caggggacac tattattagt
     3601 tggaattcct taagatttca accttatttt acagcgacag catctaatat cggttatagt
     3661 tggtggagcc atgatatcgg tgggcacatg ctgggggatt atgacgaaga gctgcaaact
     3721 agatggctac agtttggtgt ttttagtccg atcactcgat tacatagttc tagaagtcct
     3781 tttaatagta aagaaccttg gtttttttca gaaacaacat ctaagattat gaagaaatac
     3841 cttcgtttga ggcatcagat gattccatat ctatacacta tgaatgtaaa gacacacgag
     3901 gaaggtgccc cattaatcag cccaatgtat tatttctacc cagagaatga tgagagctat
     3961 aatgttccaa accaatactt ttttggaaca gaactaatgg tggctcccat tgtagaaaag
     4021 atggacttgg cattccaatc tgcaaaagtg gatgtatggt tccctgaagg tgaatggtat
     4081 gacttctttt cagagaaaaa atatacaggt ggtgtgaagt tgagtgttta tagagacatt
     4141 tcgactatac ctgtgtttgc aaaaagtggt gcgatcattc ccttggttgg ttctgagata
     4201 gatatgggtg ttgatttacc tgaagttgta gattggtatg tattcccagg caaacaacat
     4261 tcttttgaaa tgcttgaaga tcaaaatggt caaagatata aaacaagatt atcaatcgat
     4321 tgggaaatgg ggatggtaga gttaacatta caaggagatt ctagtatcgt tccaagcaat
     4381 agaaggcata gaattcattt taaaggaacg aatgtgtcta taattgaatt accaaataag
     4441 aatgatacag ctagatttga atgtaaagat aataaaacaa tatctttaaa tgatgaagtt
     4501 tttagactac taaagacagc ttctcttcca tatgaattaa aagatagatt gttaaatcaa
     4561 ttcatcaatg cgaaaaactc tcatgactta atgaatatct tgcatcatca ggataaggaa
     4621 ttgagagggc gtttgttgga aatgatattt actagccaaa actaattgat ataattagaa
     4681 agtcatttgt ttagtgtata tgaagattct gtatggtatt cgttatcact ggtcacattg
     4741 aaaatgtgaa ataagcacct acaacataga ctttacaaca cttcatgttt catatcatgg
     4801 ggtgtttttc tatttaagtc aaagccaatc aaaaaatctc actattcatc aggttaaagg
     4861 aaacctaatc atagtgagat tgtgaattat agtagagttt gtatcgcctc ttcaatttgt
     4921 tttgggtctg tttttgaaga aaagcgttca aagacttgcc catctcgacc gatgagaaac
     4981 ttagcgaaat tccattcgac tcgttttcct agtgggccgg atttctggtc ttttaaccag
     5041 acatagagag ggtctgcttc cttaccgttg accttaatct tggcaaaacg tgggaaggtg
     5101 gtttgaaaat gtaggctaca gaaggcgttg atttcctctg cgctgccggg tgcttgtccc
     5161 ataaactgat tgcaagggaa atccaatatt tcaaagccct gatcttgata gcgttcatag
     5221 agttcttgaa gtccctggta ctggggcgtt aaaccacatc cagtagcagt gttgacaatc
     5281 aagagaactt tgccacgata gctatccaag ggagttgctt ggttgttttg gttcaaaacg
     5341 gaaaaatcat aaagtgaagt catgagggcc tcttttcttt ttctacattg taacagtttt
     5401 tacttttttc gtcgagtcgg atggaagata ttgttgaaga ataagccctc aatatgatag
     5461 aaaccgttta caaataactt taaaaatggt ataatttaat taaaccggtt tacttagttg
     5521 ctataaatct ctaatttgag atttagttcc tattgatttt acaatatgtt tattggagtg
     5581 tatacatgca aacaaaaaca aagaagctca ttgtgagttt gtcttcactt gttttatcag
     5641 gatttttatt aaaccattat atgacagttg gagcggaaga aacgactacg aataccattc
     5701 agcaaagcca gaaggaagtt cagtatcagc aaagggatac aaaaaattta gttgaaaatg
     5761 gtgattttgg tcagacggag gacggaagca gtccgtggac aggaagcaaa gctcaggggt
     5821 ggtcagcttg ggtagaccag aagaatagtt ctgcagatgc ctcaactcga gtcattgagg
     5881 ctaaggatgg ggctatcact atctcaagcc ctgagaaatt aagggcagcg gttcaccgta
     5941 tggttcctat tgaagctaag aaaaagtata aactgcgttt caagattaaa acagatcata
     6001 aagtcgggat tgccaaagtt cgtatcattg aggaaagtgg taaggacaag cgattgtgga
     6061 attctgcaac gacgtcagga acaaaggact ggcagaccat tgaagcagac tatagcccga
     6121 ctttagatgt tgataaaatc aagctggagt tattctatga aacaggaact gggactgttt
     6181 cctttaagga tattgagctg gtagaggtag cagaccagct ttctgaggat tctcaaacag
     6241 ataaacagct tgaggaaaag attgatttac caattggaaa aaaacatgtt ttttctcttg
     6301 cggactatac ttataaggta gaaaatcctg acgttgcttc agtcaaaaat ggaattttag
     6361 aacctcttaa ggaagggaca accaatgtca ttgtcagtaa agatggcaag gaagtgaaaa
     6421 agattccttt gaagattcta gcctctgtta aggatgcata cacagcccgt ttggatgact
     6481 ggaatggcat catcgctggg aatcaatact atgattctaa aaatgaacag atggccaaat
     6541 taaaccagga attggaagga aaggtagctg atagcctatc tagtatttca agtcaggcgg
     6601 accgcaccta tttgtgggaa aaattttcaa attataaaat gtctgcaaat ctgactgcca
     6661 cttatcggaa attggaggag atggccaagc aagtgaccaa tccttcttct cgttattatc
     6721 aagatgaaac tgtcgttcga acagtcaggg attccatgga atggctgcat aaacatgtct
     6781 acaatagtga aaagagcatt gttgggaact ggtgggatta tgagatcggt acacctcgtg
     6841 ccatcaacaa taccttgtct ctgatgaaag aatacttctc tgatgaggaa attaaaaaat
     6901 atacagatgt aattgaaaaa tttgtaccag accccgaaca tttccgaaag acgactgata
     6961 acccatttaa ggctctaggt ggaaacttag ttgatatggg aagagtaaaa gtaatagctg
     7021 gtttactgcg taaagatgat caagaaattt cttctaccat tcgctcgatt gagcaagtgt
     7081 tcaagttggt agaccaaggt gaaggttttt atcaagatgg atcctatatc gaccacacca
     7141 atgttgccta tacgggtgct tatgggaatg ttttgattga tggcctgtct caactgttgc
     7201 cagtcattca aaagaccaag agtccaatcg ataaagataa aatgcaaacc atgtaccact
     7261 ggattgataa atcgtttgct cctttgctgg tgaatggaga actgatggat atgagtcgtg
     7321 gacgctcgat cagtcgtgca aatagcgagg ggcacgtggc cgcagtagaa gtactaagag
     7381 ggattcaccg aatagcggat atgtctgaag gagaaaccaa acaacgtttg cagagtcttg
     7441 tgaagaccat tgttcaatcg gatagttatt atgatgtctt taagaatttg aagacttata
     7501 aggatatcag tttgatgcaa tccttgttaa gtgatgcagg agtcgcaagt gttccaagac
     7561 caagttacct atctgccttt aacaagatgg ataaaacagc catgtacaat gcagagaaag
     7621 ggtttggatt tggcttgtca ctcttttcca gtcgtacctt gaattacgaa cacatgaaca
     7681 aggaaaataa acgtggttgg tatacgagtg atgggatgtt ctatctttac aatggcgatt
     7741 tgagtcacta tagcgatggc tactggccaa cagttaatcc atataagatg cctggtacaa
     7801 cagagacgga tgctaagaga gcggatagcg atacaggtaa agttttaccg tctgctttcg
     7861 ttggaacgag caaactagat gatgccaatg cgacagcaac catggatttc accaactgga
     7921 atcaaacatt gactgctcat aagagctggt ttatgctaaa ggataagatt gcctttttag
     7981 gaagcaatat ccaaaacact tcaacagata ctgctgcaac tacaatcgac cagagaaaac
     8041 tggaatcaag taatccatat aaagtctatg tcaatgataa agaagcctcc cttacagaac
     8101 aagaaaagga ttatcctgaa atccaaagtg tctttttaga atcgtccgat tcgaaaaaga
     8161 atattggtta ctttttcttt aagaagagtt caatcagcat gagtaaggct ttgcaaaagg
     8221 gagcctggaa gggtatcaat gaaggacagt cagacaagga agttgaaaat gaatttctta
     8281 cgattagtca ggctcataag caaaatggag attcttatgg ctatatgctc attcctaacg
     8341 tggatcgtgc caccttcaat caaatgataa aagagttaga aagtagcctc atcgaaaata
     8401 acgaaaccct tcagtctgtt tatgatgcta aacaaggagt ttggggcatt gtgaaatatg
     8461 atgattctgt ctctactatt tccaaccaat tccaagtttt gaaacgtgga gtctatacca
     8521 ttcgaaaaga aggggatgaa tataagattg cctactataa tcctgaaacc caggaatcag
     8581 ctccagatca ggaagtcttt aaaaagctag agcaagcagc tcagccacaa gtacagaatt
     8641 caaaagaaaa ggaaaaatct gaagaggaaa agaaccattc ggatcaaaag aatctccctc
     8701 agacaggaga aggtcagtca atcttggcaa gtctagggtt cttgctactt ggggcatttt
     8761 atctattccg tagaggaaag aacaactaat ttgttcatca tctagatgaa ttacatgaaa
     8821 ctgtggaaaa tagaaaaaat ccgaagaagg ccaaatatct tcggattttt cttttaaaaa
     8881 aggataagtt gatacttatc cttttttact tcattattat gtgactatag cgttttgaaa
     8941 ctagaataga acgttataac tgctaaaaga tttctataaa ttcatttgat tttcctaatc
     9001 aatttgttcg tatcctattt cactccacta tataagtccg tctcgctccg tgaggtgcgc
     9061 gtcgaaggtt ataccaaaaa aactccaaac gcgatacaat aaaggtattc aatccaattg
     9121 taaagcgaaa ggagaaaaaa tatggcacaa aagactcata gtttatcata cacaaagtgg
     9181 atgtgttcta tcatattgtg ttcaccccta agtatagacg aaaagtcatc tataatcaat
     9241 atagaagtag tttaggcgaa atatttcatc gcttgtgtag ttataaaggt gttgaaatga
     9301 tcgagggtca cttaatgcca gaccatgtac acatgttagt cagtattccg tcgaggctaa
     9361 gtgtttcaag ttttatgggg tatttaaaag gcaaaagtgc actcatgatg tttgacaaac
     9421 acgccaatct caagtacaag tttgggaatc ggaatttctg ggcggaaggt tattatgtaa
     9481 gtccagtagg gcttaatgaa gccacaatta agaaatatag tcaagattaa agaaattatc
     9541 gagtggatga tttcttcacg agtatgaaaa tttgagaact agtaaagtat gatatatagt
     9601 aaaatgaaat aagaacagga cgaaatcgat caggacagtc aaatcgattt ttaacaatgt
     9661 tttagaagta gaggtatact attccagttt caatctacta tagcactaga taaattgagt
     9721 gtaaaagaat atgaggatcc ctttagggat agtggtaagt aataccaaag tctcttaaag
     9781 aggcaagtga cgagtcaaga gcaataaggc ttgaacaacg tgaaagccag cgtctttagg
     9841 cgctggctga tgatttgggc ttatagctct gagataaacc acccgttaga cgggtagtta
     9901 tgattttatc tgagtgtaat atactgttgg gcaatctcgc tgatgcggtc aaagttgcct
     9961 tgggaagcga gtttattgag ttcgccacca attccaacgg catctgcacc agcagcgaac
    10021 cattgaggga tgttgtttag gccgactcct ccggttacca ttacggaaac ttgtgggatc
    10081 ggtgccttga ctgcagagat atatgctgga ctgagagtac tacctgggaa gagtttgatg
    10141 atttcactac cggcttcaag tgcagtcgtg atctctgtga gggtaataca gcctggaatg
    10201 tacggtgtgc tgtagagatt gcacattttc gcagtttcag catggaaaga tggagaaaca
    10261 acgtaatttg ctccagctag aatggcatct ctagcagtta cggcatcaag cacagtacct
    10321 gcaccgatac aaacactctg atcgtcctga tacaagtcta caagttcctt gatgatttgt
    10381 cctgcatact gattggtata ggcgatttca atagctttga taccgccctt gatacaagca
    10441 atcgaggctt gtagtccttc ttcctttgta tttcctcgaa tgacagcgac aattttctgt
    10501 ttttttagtt caataatcgt atctgatttg gtcatgtaat tctcctaacg aatgatatct
    10561 tgtgcatttg ccagtaaatt ttcaatacta gttgcggaag tggagagatg gtctcctgga
    10621 agagtgcatt tgagagttgc gctcgcaact gcaaagtcaa tggtagtttt tagggaggaa
    10681 tgatggagta gttggtaaag ggcaccagat ataaaggcat ccccgctacc aattcgttga
    10741 tagactgcag tttttagttg gacagactct tcaaatcgtt cttccagagc ataggcttga
    10801 tagacatttt tgtcttgctc atcactagat cggagggtat ggaaaatagc cttgaaacca
    10861 taggtttctt ttaaaagtcg catgcgattt tccacctctt ctaggctagc actgtctctt
    10921 ggaaacatct ctagattttg gtcatcaatc atgagaggat caataccgaa gcaatagtca
    10981 gtaaaacgtg caaacttaga aaattcatac ttggcttcta gggctgaaat catctttgtt
    11041 ctcagattga gatccattga aacgacaatt cctcggcgct tggcttcttc caagagtagg
    11101 agaaggatcg cacggacctc ttgaccgata gctacggtga ttccactaaa atgaaaatgg
    11161 ctaatcccct gaaagagaga atccatatct agcatgtttg gccgaatctg gctgatactc
    11221 gtatgcttac gatcgtagaa aacttcactt tgacgacaac caaagccgtt ctccaaatag
    11281 tagaggccga ttcgatcgcc aagccgacag attgaactgg tatcgatttg gtgctgtttc
    11341 aagaatgtga gaaaacgatc tccaatctcg ttggcaggga gtgcggtaaa gactttcgtt
    11401 gagataccca gggcttgcaa attacaagcg atgttaattt ctgatccgcc aaaataagtc
    11461 gaactggcaa catgatcgcc gatactggtg gcatctaatg gtgagattcg aattaatggt
    11521 tcgccaaaca gtaatatttt cgtcatccat tagttccttt ctggtttaga atagctttac
    11581 ttggctaata cttctttcaa ataagcagcg acttcatcat cttggcagtt ttcaaagaag
    11641 tattcttgga aatatttgcc agagatggtg tctttgagga agtcttggtc gattgtttta
    11701 aggatggtca tcaaatcatt gtgggtgatt tgtttcacct cgtttaagat acgagcgttg
    11761 cgttgttcag ggattacacg ttctcttggg tagccaccgc ccatttcttc agcaaataag
    11821 cgttcaaaca tcaatttgag ggtcagttct gccccccagc caaatccttt ggcataaggg
    11881 atagacaagg cgttaccacc attgatttga gaataaaggt aagcgtcagt tgggtccact
    11941 gctagaccac agacaacacc agggaagctg tttaaagcaa gcatagcccc tacacccgta
    12001 ccacagccgg taacaacaaa gtcaactgcc tttgtattta aaaggatggc agccattagt
    12061 ccgttctgca cataagttaa ttgactttct ccttcttctc cacgcatacc atagttaaat
    12121 aattggtagt cttttttatc tgtcgcttct tttagactat cgtaaataat gtgattcttg
    12181 ctagcttgac tattttcatt gattaatgcg attttcataa gatcttcttt ctatttttac
    12241 tcaggttgtt ttccgatgta ggctaagata ccgccatcta catataggat gtggccattg
    12301 acaaaattgc tggcatcact agcgagaaag acagcagggc ccatcaaatc ttcaggattt
    12361 ccccaacgtg cagcaggtgt ttttgcaatg atgaactggt caaatgggtg gcgagaacca
    12421 tcttcttgca actcacgaag aggtgctgtt tgaggagtgg caatataacc tggtccaatt
    12481 ccgttacatt ggatattggc tccaccgtat tcagacgcaa tgttgcgggt caacattttc
    12541 aagccccctt tagcagcagc ataagcgcta actgtttcac gtcccagttc gctcatcatc
    12601 gaacaaatat tgataatctt tccatgccct ttctttatca tagaaggaat aactgccttt
    12661 gaaacgataa atggtgcgtt taagtcaata tcgatgacct tacggaaatc agcggcgctc
    12721 atttcgcaca ttggaactcg gcggataata ccagcgttat taacgaggat gtcaatgaca
    12781 ccaacctctt gttcgatttg cttgaccatg gcttggatac cgtcctcgtc tgtcacgtca
    12841 cagacatatc catgggcttg gatgccaact tcacgataag cttcaatccc tttattgacc
    12901 agatcttgat tgatatcgtt aaagacaata gtagcgccgg cttcagcgta ggatttggca
    12961 atagcaaatc caattccata agaagcgccg gtgatgagag caatttttcc ttttaaagaa
    13021 aactgctcaa ttgagaatga tgtatttgtc atttgatact tcctttcaaa tttgttaaga
    13081 cttctctata accaaattat attctagtaa accggttttg tcaataaata aatattttta
    13141 tttataaaaa gatttatttt cctaaaaaaa acggaagatt ttgaaatttc tgtctattaa
    13201 aat
//
LOCUS       DAVKWS010000051        12955 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKWS010000051 DAVKWS010000000
VERSION     DAVKWS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.051"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..370
                     /locus_tag="VW852_001637"
     CDS             <1..370
                     /locus_tag="VW852_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0272749.1"
                     /translation="GAMATGWAKVNGSWYYLNANGSMATGWLQYNGSWYYLNANGAMA
                     TGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLG
                     ALAVNTTVDGYKVNANGEWV"
     gene            771..1892
                     /gene="mnmA"
                     /locus_tag="VW852_001638"
     CDS             771..1892
                     /gene="mnmA"
                     /locus_tag="VW852_001638"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV0272750.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTNLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            2033..2488
                     /locus_tag="VW852_001639"
     CDS             2033..2488
                     /locus_tag="VW852_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0272751.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2498..4411
                     /gene="mnmG"
                     /locus_tag="VW852_001640"
     CDS             2498..4411
                     /gene="mnmG"
                     /locus_tag="VW852_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV0272752.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNIKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4618..4710)
                     /locus_tag="VW852_001641"
                     /pseudo
     CDS             complement(4618..4710)
                     /locus_tag="VW852_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4764..6443)
                     /locus_tag="VW852_001642"
     CDS             complement(4764..6443)
                     /locus_tag="VW852_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0272753.1"
                     /translation="MTYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTTDSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6445..6678)
                     /locus_tag="VW852_001643"
     CDS             complement(6445..6678)
                     /locus_tag="VW852_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV0272754.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            7060..7281
                     /locus_tag="VW852_001644"
     CDS             7060..7281
                     /locus_tag="VW852_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272755.1"
                     /translation="MTNQVLEENYFCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7494..7646)
                     /gene="cibB"
                     /locus_tag="VW852_001645"
     CDS             complement(7494..7646)
                     /gene="cibB"
                     /locus_tag="VW852_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV0272756.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7648..7833)
                     /gene="cibA"
                     /locus_tag="VW852_001646"
     CDS             complement(7648..7833)
                     /gene="cibA"
                     /locus_tag="VW852_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV0272757.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            8011..8694
                     /gene="tsaB"
                     /locus_tag="VW852_001647"
     CDS             8011..8694
                     /gene="tsaB"
                     /locus_tag="VW852_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV0272758.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETMINIKKNHSINLMPAID
                     FLMASLDLTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPR
                     QKEGLVVPIMDARRNNVYAGFYENAKPVLPEAHLSFAEVLEQVKDAEQVTFVGEVGAF
                     VEQIQEQLPQASYQETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8691..9128
                     /gene="rimI"
                     /locus_tag="VW852_001648"
     CDS             8691..9128
                     /gene="rimI"
                     /locus_tag="VW852_001648"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV0272759.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            9118..10128
                     /gene="tsaD"
                     /locus_tag="VW852_001649"
     CDS             9118..10128
                     /gene="tsaD"
                     /locus_tag="VW852_001649"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV0272760.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10168..11474)
                     /locus_tag="VW852_001650"
                     /pseudo
     CDS             complement(10168..11474)
                     /locus_tag="VW852_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11750..12101)
                     /locus_tag="VW852_001651"
                     /pseudo
     CDS             complement(11750..12101)
                     /locus_tag="VW852_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12208..12501)
                     /locus_tag="VW852_001652"
                     /pseudo
     CDS             complement(12208..12501)
                     /locus_tag="VW852_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3677 a         2576 c         3020 g         3682 t
ORIGIN      
        1 cggcgctatg gcaacaggtt gggctaaagt caacggttca tggtactacc tcaacgctaa
       61 cggttcaatg gcaacaggat ggctccaata caacggttca tggtactacc tcaacgctaa
      121 cggtgctatg gcaacaggtt gggctaaagt caacggttca tggtactacc taaacgctaa
      181 cggttcaatg gcaacaggtt gggtgaaaga tggagatacc tggtactatc ttgaagcatc
      241 aggtgctatg aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg
      301 cttaggtgcc cttgcagtca acacaactgt agatggctat aaagtcaatg ccaatggtga
      361 atgggtttaa gccgattaaa ttaaatcatg ttaagaacat ttgacatttt aattttgaaa
      421 caaagataag gttcgattga atagatttat gttcgtattc tttaggtacc tatcttatga
      481 tttcaggaaa tgtcattaaa aaaacgactc cttttctcta acttgaaaaa tagattagag
      541 aaaatgggtt gttttattta ttatagttat ttgaatgaag ataagaagaa ggtatactca
      601 cgtcattcac ataatctgta tattgactat aagttttaaa aaacaatttt taagctcttc
      661 cttgtcttct ctaaccaagc gtgttataat gaatactgct caagcgacct tcaatcgtga
      721 agcacacacg accttcaatc gtgaataaac gaatagatgg gagacttacc atgagtgata
      781 actctaaaac acgtgttgtc gtggggatga gtggtggtgt tgattcgtcg gtgacggctc
      841 ttcttctcaa ggagcagggc tacgatgtga tcggtatctt catgaagaac tgggatgaca
      901 cagatgaaaa cggcgtctgt acggcgaccg aagattacaa ggatgtggtt gcggtggcag
      961 accagattgg cattccctac tactctgtca attttgaaaa agagtactgg gaccgcgttt
     1021 ttgagtattt cctagcggaa taccgtgcag ggcgcacgcc aaatccggac gttatgtgca
     1081 acaaggaaat caagttcaag gcctttttgg actatgccat gaccttgggg gcagactatg
     1141 tagcgactgg gcactatgct cgagtggcgc gtgatgagga tggcactgtt cacatgcttc
     1201 gtggcgtgga caatggcaag gatcagacct atttcctcag ccaactttcg caagaacaac
     1261 ttcaaaaaac catgttccca ctaggacatt tggaaaagcc tgaagttcga aaactagcag
     1321 aagaagcagg tctttcgact gctaagaaga aagactcgac agggatttgc tttatcggag
     1381 aaaagaactt taaaaacttt ctcagcaact acctgccagc tcagcctggt cgtatgatga
     1441 ctgtggatgg tcgtgatatg ggcgagcatg ctggtcttat gtactataca atcggtcagc
     1501 gtggcggact cggtatcggt gggcaacacg gtggtgacaa tgccccttgg ttcgttgtcg
     1561 gaaaagacct aagcaagaat attctctatg taggccaagg tttctaccat gattcgctca
     1621 tgtcaactaa cctagaagcc agtcaagtcc actttactcg tgaaatgcca gaagagttta
     1681 cgctagaatg tacggctaaa ttccgttacc gtcagcctga ctctaaggtg accgtccatg
     1741 tcaaaggaga caaggcagaa gttatctttg cggaaccaca acgcgcgatt acaccaggac
     1801 aggcagttgt cttttacgat ggcgaagagt gtctaggtgg cggtttgatt gacaatgctt
     1861 accgcgatgg acaagtttgt cagtacattt agattgacaa attttctcaa tttgctacaa
     1921 taataacagc aatagaaatg atggtcaaag ctcatggatg ttgcaggctt ttttgtcctg
     1981 cacttctttg tagttttgac tgtttttgtg tcgtttaagg gaaaggacaa gaatgactca
     2041 acaagacttt cggacaaaag tagacaatac ggtttttggt gttcgggcga cagccttgat
     2101 tgtccaaaat cacaagcttc tagtcaccaa agacaagggc aagtattaca ctatcggcgg
     2161 tgcgattcaa gttaacgaaa aaacggaaga cgcggtagtc cgtgaagtga aggaagaact
     2221 aggtgttaaa gctcaagctg ggcagctagc ttttgtggtt gaaaatcgtt ttgaagtgga
     2281 cggcgtttcc tatcacaata tcgagtttca ttatctggtg gatttgcttg aagatgcccc
     2341 gttgaccatg caggaagatg agaaaaggca gccctgtgag tggattgact tggataagct
     2401 tgaggatatc cagctagttc cagccttttt aaaaacagcc ctaccagatt gggaaggcca
     2461 actaagacac attcatcttg aggaatagga gagaaacatg acttatcatt ttactgaaga
     2521 atacgatatt attgtaattg gtgcgggaca cgctggggtt gaggcttcct tggccgctag
     2581 ccgtatgggc tgtaaggtcc tgcttgcgac catcaatatt gaaatgctgg ctttcatgcc
     2641 ttgtaatccc tctatcggtg gttctgccaa ggggattgtc gtgcgtgaag tcgatgccct
     2701 cggtggcgag atggccaaaa ccattgacaa gacttacatc cagatgaaga tgctaaacac
     2761 agggaagggg ccagctgtcc gtgcccttcg tgcgcaggct gataaggaac tttactctaa
     2821 ggaaatgcgc aagacagttg aaaatcaaga aaatctgacc cttcgtcaaa ccatgattga
     2881 tgagattttg gtggaagatg gcaaggttgt cggtgtgcgt acagccaccc atcaagaata
     2941 tgctgctaag gctgttattg tgacgacagg gactgctctc cgtggggaaa ttatcatcgg
     3001 agacctcaag tactcatcag gtcctaacca cagcttggct tctattaacc tagctgacaa
     3061 tatcaaggaa ctgggcctcg aaatcggtcg tttcaagaca ggaacccctc cacgtgtcaa
     3121 ggcctcttct atcaactacg acgtgacaga aatccagcca ggagatgaag cacctaatca
     3181 tttctcatac acttcacgtg atgaggatta tgtcaaagat caagtgccat gctggttgac
     3241 ctataccaat ggtaccagtc atgagattat tcaaaacaac ctccaccgtg cgcctatgtt
     3301 tacaggtgtg gtcaagggag tggggcctcg ttactgtccg tcgattgaag acaagattgt
     3361 gcgctttgcg gacaaggaac gtcaccaact cttccttgag ccagaagggc gcaatactga
     3421 ggaagtctat gtgcaaggac tttcaaccag tctgcctgag gatgtccagc gtgacttggt
     3481 tcattccatc aaaggtttgg aaaatgcaga gatgatgcgg acaggttatg ctattgagta
     3541 tgatatggtc ttgcctcatc agttgcgtgc gactttggaa accaagaaaa tctcaggtct
     3601 cttcactgct ggtcagacaa atggaacatc aggttatgaa gaagctgctg gccaagggat
     3661 tatcgcgggt atcaatgcgg ctctgaaaat ccaaggtaaa cctgagttga ttttgaagcg
     3721 cagtgatggt tatatagggg tgatgattga tgacttagtg accaagggaa ccattgaacc
     3781 ttaccgtctc ttgaccagtc gtgctgaata ccgtctcatt cttcgccatg acaatgccga
     3841 tatgcgtttg acagagatgg gccgtgagat tggccttgtg gatgatgaac gctgggctcg
     3901 ttttgaaatc aagaaaaatc aatttgataa tgagatgaaa cgcctagaca gtatcaaact
     3961 caagccagtc aaggaaacca atgctaaggt tgaggaaatg ggcttcaagc cgttgacaga
     4021 tgcggtgaca gccaaggaat tccttcgccg tccagaagtt tcttaccaag atgtggtggc
     4081 cttcatcgga ccagctgcag aagacttgga tgacaagatt atcgaattga ttgaaacaga
     4141 aatcaagtac gaaggctata tttccaaagc catggatcag gttgccaaga tgaaacgtat
     4201 ggaagaaaaa cgcattccag ccaatattga ctgggatgac attgactcta tcgcaacaga
     4261 agctcgtcag aagttcaaac tcatcaatcc agaaaccatc ggccaagcca gccgtatttc
     4321 gggagtaaac ccagcagata tttctatttt gatggtgtat ctggaaggta aaaatcgtag
     4381 tatttctaaa actcttcaaa aatcaaaatg atacgtcgtc ggcttcttac gaatgagttc
     4441 aaagcttggc tttgattcat ctccaacctc ccatagttcc ccgaactatg ggagctaact
     4501 caagttatgc tttcgcttag ccttctagtc tgatttcgat ttttattgat actcttcgaa
     4561 aatctcttca aaccgcgtca acgtcgcctt gccgtaggta tggctactga cttcgtcagt
     4621 tctatctaca acctcaaagc agtgctttga gcaacctacg gctagcttcc tagtttgctc
     4681 tttgattttc attgagtatg agttttaatc tcaaaagaaa gcatagagaa aaacagctcc
     4741 gaggacgggg ctgttttctt gctttattct tcatctggtg tgaggatcat cgggatgatg
     4801 atcggttcac gttcggtatt ttcatagagg aaggggcgaa tagcgttgac aatggcacca
     4861 ttgacagatt gcacgctagc atccttattt ttcagtgcga tacgaatggc attgaagagg
     4921 atacgctggc tttggcgaat caagtcgcca gactctctca tgtagacaaa gcctcggctg
     4981 aggatgtctg gaccagatag aatcatctgc gatttgaagt caacagttgc aactgccaga
     5041 acgacaccgt cttcagatag atcgcgacga tctttgagga cagctgcgcc aatttcaccg
     5101 atacgatttc catcgacata gatatcttgg gcgttgaaat gacctgcgat acgagctgag
     5161 tcagtagtaa gggcaagcac atcgccattg ctcatgataa agatattgtc cttctcaaca
     5221 ccagtatcca ctgctagtcc agcgtggact ttttgcatgc ggtattcacc gtggacaggc
     5281 atgaagtatt ttggcttaat caagcagagc atgagttttt gctcttgctg accaccgtgt
     5341 ccagatgtat ggatattgtt cactttaccg tggataactt cgacaccagc ttcagaaatg
     5401 atgttaatca gcttgttgac gctagtagtg tttccaggga tgggactaga agagaagata
     5461 acggtatcac ctggttgtaa ttgtacttga cggtgggttc cgttggcgat acgagagagg
     5521 gctgccatag gctcaccctg actacctgta cagaggataa gaacttctcc tgcaggataa
     5581 tctttgattt catttggctc gataaaggtt cccttaggag ctttgatgta gccaagatcg
     5641 attccgttga caatggcctt ttccatagaa cgaccaaaga ccgcaatctt gcgtccagtc
     5701 ttaacagcag cttctgttgc ctgctggaga cggaagatat ttgaggcaaa ggatgcaaag
     5761 atgatacgtc cttcaatacc ttggataatc ttcataatgg actgaccaac gactttttca
     5821 gagttggtaa aggttggtac ttccgcattt gtcgagtcag acaggagaca gagcacgcct
     5881 tcttcaccaa gcgcagccat acgatgcaag tccgcaggtt ctccaactgg agtaaagtcg
     5941 aacttaaagt cacccgtaca gacgattttc ccttgaggag tatgaatgac aatccccaaa
     6001 ggctctggaa tagagtgagt cgttctaaag aaagttgcct tgagattttt aaaggtcaac
     6061 tcggtgttgt ggttgatttc gtaaagtttg gcgttgcgca agaggccgtg ttcttcgagt
     6121 ttcccacgga tcaaagccaa ggcaagcggt ccagcgtaga tagggacatt tgcttgcttg
     6181 agtaggaacg gaatcccacc aatgtggtcc tcgtgtccgt gtgtgattaa aacagccttg
     6241 acgcggtcga tattgtccac gatgtaagag tagtcaggaa tgacatagtc gataccaagc
     6301 aagtcatctt ctgggaattt aatcccagca tcgacgatga taatctcgcc ttggtattca
     6361 attccgtaag tgtttttccc gatttctcct agaccaccga tggcaaaaac gccgacttct
     6421 tcaggtttaa gagtgtaggt catattagaa ctccgtaatt tcgaaggcgc cagtttcttt
     6481 ttcgtaatcg agcaatttgt cagacaagag ttcgatatat tcgatattgt actctgggcg
     6541 attttcttcg acaagttggc gagcagtgat acggccctca agttctgagc tggcatcgat
     6601 gtctaggtaa agcgcgcgtg ttgtttcacg gcgtgggcta cgttcttttg tttcttgata
     6661 aaaaactttg taaatcatat agttcctttc tatttacagg tcagcttatt ccaaactttt
     6721 agcagatatt tgcttgattt cattccattt tgtgtctaga ttgaccttga ttcgttacaa
     6781 ttatttcaat tataccacaa aatgaaaaat tagtaaaaga cggaaacgag aaagttaggg
     6841 gacgatttct tcggtaagcg tttgcgaaaa taaagaaaat gtattatgtt gataacggaa
     6901 gtgaaattta ttgcttgtca taattccata tagattttaa gtcaaggagg ttgccctatg
     6961 tataacttat ttgcttttgc tatcggagtt cgtttgctag cttttatcgt ttttgttttg
     7021 cgtttatgga ggaaatcttc tatttgccaa actggaacga tgacaaacca agttcttgag
     7081 gaaaattact tttgtgactc tctactcgct ttttctcctc ctcgtgacct atgtcgtttg
     7141 gtttgtattt tactttggat taaatgctta gatgccctgc ttatgctggg gttttttgtt
     7201 tggggatgta gagatagtag atttacgaca cgaaaattcc accccatcta tcaagatgag
     7261 gtggtctttg catttttcta aaatgatatg acaatggaat tttcaaagct tattattttt
     7321 atgggagtca ttgttctgct caaaaataga gttgaagcat aaaaataaaa caatacaaac
     7381 agataaatcg aatggtaaag ggatggtagt aatgttcaaa tcattgagaa tgctgagcaa
     7441 actgtaagag atgaagacaa taaaatttat ccttctaaaa atgtgtttct tatctagcat
     7501 gacttaccaa actttttacg aaggtctttt ccaatggtat aacctgccat aaataaagcg
     7561 ataccaacac ctacaccaaa gccacccttg atttcttgca attccttatt agaaatttca
     7621 cgatagttgt tcaaatcttt catcatatta atctcctcct aaaaaataac cagctgccaa
     7681 accagaacct ataagtgctg ttccacctgc aatagccttc caagatactg ctactccaaa
     7741 gataaccaag ggagcgagac cgccatcaat atctgataat tcattttcag ataaggataa
     7801 aaattgattg tcaagaatgt caaaatttgt cattgaactt tcctcctttt ttcttgttca
     7861 cttttatatt cggaaaagaa aaggaaaagt gaacattttt ttaaattatt tttagttaag
     7921 tgagggcact cttgtctggg ggcaaagaaa attcccatgt gccagctttt gtagtataat
     7981 agtaagcaga caaaaagata ggaatgtgtt atgaaagtat tagcttttga tacgtccagc
     8041 aaggctcttt ctctcgctat tttagaggac aagcaggttc ttgccgagac tatgattaat
     8101 atcaagaaaa accacagtat caacctcatg cctgccatcg attttttgat ggcgagtctg
     8161 gacctgacac ccaaggattt ggatcgcatc gtggtggcag agggaccggg tagctataca
     8221 ggtttgcgaa ttgcggtagc aactgccaag accttggctc ataccctgaa cattgagtta
     8281 gttgggatgt ctagtctatt ggctctggtg ccacgccaaa aagaaggctt ggtggttcct
     8341 atcatggatg cgcgtcgcaa caatgtctat gcaggctttt atgaaaatgc caagccagtc
     8401 ttgccagaag cgcatctgtc ttttgcggaa gtgctagagc aggtcaagga tgctgagcag
     8461 gtgacctttg tcggagaagt tggagccttt gtggaacaga tccaagaaca actaccacaa
     8521 gccagctacc aagaaacctt gccaaatgca gctaatctag ctctttgggc ctgggacaaa
     8581 gaagcagact ccttgcacga ctttgtgcca aattacctaa agcgtgtcga ggctgaggaa
     8641 aactggctca agaaccatac cgagtctggc gagtcttaca ttaaacgcct atgatagaaa
     8701 tcaagcgaat tcaacaacag cctgacctag ctcaagccat ctacgctgtt atggcagctg
     8761 tttacctagt cagtccttgg actctggagc aaatccaagc agatctgtcc caagaccaga
     8821 cttggtatgc attggcttat gatggggcag aagtgattgg atttctagct gtgcaggaga
     8881 atctttttga agcagaagtc ctgcaaatcg ctgtcaaagg agcctatcag ggtcagggaa
     8941 ttgcgtcagc cttgtttgct caattgccga cagataagga gattttcctc gaagtcagac
     9001 agtcaaatca acgagcgcaa gcattttaca agaaagaaaa aatggcggtc atcgctgagc
     9061 gaaaggccta ctaccatgac ccagtcgagg acgccattat catgaagaga gaaatagatg
     9121 aaggatagat atattttagc atttgagaca tcctgtgatg agaccagtgt cgccgtcttg
     9181 aaaaacgacg atgagctctt gtccaatgtc attgctagtc aaattgagag tcacaaacgt
     9241 tttggtggcg tagtgcccga agtagccagt cgtcaccatg tcgaggtcat tacagcctgt
     9301 atcgaggagg cattggcaga agcagggatt accgaagagg acgtgacagc tgttgcggtt
     9361 acctacggac caggcttggt cggagccttg ctagttggtt tgtcagctgc caaggccttt
     9421 gcttgggctc acggacttcc actgattcct gttaatcaca tggctgggca cctcatggca
     9481 gctcagagtg tggagccttt ggagtttccc ttgctagccc tcttggtcag cggcggacac
     9541 acagagttgg tttatgtttc ggaggcagga gattataaga ttgttgggga aacccgtgat
     9601 gatgcggttg gtgaggctta tgataaggtc ggccgtgtca tgggcttgac ctatcctgca
     9661 ggtcgtgaga ttgacgagct ggctcatcag gggcaggata tttatgattt cccccgtgcc
     9721 atgattaagg aagataatct ggagttctcc ttctcaggtt tgaaatctgc ctttatcaat
     9781 ctttatcaca atgccgagca aaagggagaa agcctgtcta cagaagattt gtgtgcttcc
     9841 ttccaagcag cagttatgga cattctcatg gcaaaaacca agaaggcttt ggagaaatat
     9901 cctgttaaaa ccctggttgt ggcaggtggt gtggcagcca ataaaggtct cagagaacgc
     9961 ctagcagccg aaatcacaga tgtcaaggtt atcatccccc ctctgcgact ctgcggagac
    10021 aatgcaggta tgattgccta tgccagcgtc agcgagtgga acaaagaaaa cttcgcaggc
    10081 tgggacctca atgccaaacc aagtcttgcc tttgatacca tggaataaag agtggctctt
    10141 tgtcaagtgt agtgggtagg cgaaaagcta caatctggag agtacgaaat tcgtactctc
    10201 ttttttgatg ttcaaagtca tcaaaatccg ttttttaaaa ttgttaaagt tccggaaacc
    10261 aaaggctttg cgcttgataa ccttaatcaa attattggtc gcttcaagtt tagcgttgga
    10321 ataaggcagc ttaagtgcgt tgataatctt atctctatcc cttaaaaaag tccaaaagac
    10381 cgtttgaaaa agcggatgaa ccgttggcag ttcttgctca atcaatccaa agaaatggtc
    10441 ggcattcttc tcttgaaaat gaaagagcag gagttgatag agttcgtagt gaacctgtaa
    10501 tccctcagag taagaaagca gcttatctcg tacttcccga tgggttaagt gcattcgaaa
    10561 cgtggactga tagaatcgtt tctgacagag cttgaagcta tcctggtgta gtaaatcagt
    10621 ccagtggact gatttagccc gagcctagaa acgaaaaagc gagggttcca aaagcgcttg
    10681 agcgcccgat actccaagga ttttcggtca aattggttca tgatttggat tcgtactcgg
    10741 ttcatagctc ggctcagatg ttggacaatg tggaagcggt caagaacaat cttcgccttt
    10801 ggaaagagtt gcttagcgag ccgataataa gggctgtaca tgtccatggt gatgacctcg
    10861 accagctccc gaacctctct ctgatagcgt tggaaatggt ttcgaatcac cgcatgagtt
    10921 cgcccgtcta aaattgcgag gatggatttg gactcaaaat cttgggcaat gaagctcatt
    10981 ttgctctttt tgaagctata ctcatcccaa ctcataactt ttggcaactt ggtccaatcg
    11041 gtttcaaact taaattccct cagttttcgg atgacggtgg aagttgagac cgccaatctg
    11101 tgggcaatct cagtcataga ctgcttttca aggaggagct gagcgatttt ttgtctcacc
    11161 atgttggaaa tctggcaatt tttcttgaca atggatgttt gagaaacgac caccttaagg
    11221 caattcttgc actgaaagcg gcgctttttg agatgtagta ccgtgggtaa accctgacag
    11281 tcgagaagcg gaattttaga ggctttctgg aagtcgtatt tgatcatctt cccttgacaa
    11341 tgaggacaag gaggagcggg ggaatccaac tttgcctgaa cgactagatg ggtttggtat
    11401 ttcagaacag acaagatttt gatatttttg tctttcaatc cgagcaaatc tgtggtattc
    11461 ttaagttgtt ccatatgacc cctttctaat gagttggtta gacgcttttc attataggtc
    11521 atatggggct ttttttctac aagaaacgac cctataattc ctggggtggg attacccact
    11581 acagaaatta tagagccaaa acattccaaa gtcttgtctg actctggaat gttttctgtt
    11641 cacagttgtt ttgtaactgc ctaataagta accgcgctat aacgaggtat ctaccgagat
    11701 tgtcaattct ccagtatact gttataaaaa acaaaactgg aggatttttt tatgtccccc
    11761 catcgtccca tcgactgttc cgtagctgcc caataacgga gtatagtcgg ctgaaacaag
    11821 aataggacaa aagtgcctca agaaaagcat cgtaatttga caatattatt ctgagatgtt
    11881 tttgatgtaa gcccatattt tctcaatggg attatactcg ggtgaatagg taggaagtgg
    11941 taacagtcta tgccctgctc cttacacatg ttcattctgt gaaaccttgc attgtccata
    12001 atgataaggg atggtgtatc taaagtaggt agtaagaatt tttggaagca agcttcgaaa
    12061 aagtcactcg tcatcgtatc tttataggtc atcggagcca tagtatattg aaacaaaaaa
    12121 gggcattagt acttctataa cagtatcgca atctctggtg tgcgttgtct cgatcttaat
    12181 tcaaataatt atatcttctt ccagagagac taactgcctg ctcaaggctc gatcatattc
    12241 gagataaaaa taggtctcaa cccctgtctc ataaatataa ataggagtca ggtagcttaa
    12301 gtgattcaat tctttaagga accgatttac tttttctggg tcttgttcgc agtaggtaca
    12361 gctctttttt agactatatc ccatagcctt tagagcgtaa tgaatagttg ttggaggaca
    12421 gttgaattca gcagctattt cagtcaaata agcatcggga tgagtttcaa tatagatctt
    12481 taatttatct ctatcaactt tttcaattaa tagtggaagt tttcttaaag ttagtgtgtt
    12541 gaaacgtttt tatacagtgc taaaatttga agtgtagtgc agatgtattt gtaatagtat
    12601 tatagcaatt ctttggagaa ggtcgagaca agagaaaata ggtatctttg agatataagc
    12661 atgatttact ataaaattat tgagttattc ataaaaattt ctctatcaaa tatcataatg
    12721 cgtcgtttaa ggagatggtt acaatgcaat atagttgtgg aaaaataaat attaatatcc
    12781 ctgatggata tggagacatt aaggatatag tgttttcggc tcatattata gttaggtata
    12841 ataatggaca ttgtggaggt atagatccgc atataattgg actttgtaaa aagcagatca
    12901 gaaggatgtc tctatatcct attttgataa tcgtatctag agattcaaag gttat
//
LOCUS       DAVKWS010000052        12906 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKWS010000052 DAVKWS010000000
VERSION     DAVKWS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.052"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..423
                     /locus_tag="VW852_001653"
     CDS             <1..423
                     /locus_tag="VW852_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0272761.1"
                     /translation="ANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNYSHDMKLFKMSC
                     RNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLIAEDKAYNHALSKERSKVENI
                     FAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            615..2906
                     /gene="pcrA"
                     /locus_tag="VW852_001654"
     CDS             615..2906
                     /gene="pcrA"
                     /locus_tag="VW852_001654"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV0272762.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNTQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2949..3629
                     /gene="radC"
                     /locus_tag="VW852_001655"
     CDS             2949..3629
                     /gene="radC"
                     /locus_tag="VW852_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV0272763.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3626..4315)
                     /locus_tag="VW852_001656"
     CDS             complement(3626..4315)
                     /locus_tag="VW852_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0272764.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4333..4974)
                     /locus_tag="VW852_001657"
     CDS             complement(4333..4974)
                     /locus_tag="VW852_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV0272765.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(5057..5272)
                     /locus_tag="VW852_001658"
     CDS             complement(5057..5272)
                     /locus_tag="VW852_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0272766.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5280..5627)
                     /locus_tag="VW852_001659"
     CDS             complement(5280..5627)
                     /locus_tag="VW852_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV0272767.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5632..6747)
                     /locus_tag="VW852_001660"
     CDS             complement(5632..6747)
                     /locus_tag="VW852_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0272768.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMH
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6757..7716)
                     /locus_tag="VW852_001661"
     CDS             complement(6757..7716)
                     /locus_tag="VW852_001661"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0272769.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7829..8398)
                     /locus_tag="VW852_001662"
     CDS             complement(7829..8398)
                     /locus_tag="VW852_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV0272770.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTLDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8526..9197
                     /locus_tag="VW852_001663"
     CDS             8526..9197
                     /locus_tag="VW852_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV0272771.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRSITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9181..9999
                     /locus_tag="VW852_001664"
     CDS             9181..9999
                     /locus_tag="VW852_001664"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV0272772.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9996..10892
                     /locus_tag="VW852_001665"
     CDS             9996..10892
                     /locus_tag="VW852_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0272773.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSYIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10936..11910
                     /gene="pta"
                     /locus_tag="VW852_001666"
     CDS             10936..11910
                     /gene="pta"
                     /locus_tag="VW852_001666"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV0272774.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(12310..12609)
                     /locus_tag="VW852_001667"
     CDS             complement(12310..12609)
                     /locus_tag="VW852_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV0272775.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         3770 a         2453 c         2750 g         3933 t
ORIGIN      
        1 gcgaatcatt ctggtaaaaa gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt
       61 caagggagaa ttgtttcttt ggatatcgct gtgaactata gtcatgatat gaagttgttc
      121 aaaatgagtt gcagaaatat cggacaagct ggtaaaatct tggctgacag tggttatcaa
      181 gggctcatga agatatatcc tcaagcacaa actccacgta aatccagcaa actcaagccg
      241 ctaatagctg aagataaagc ttataaccat gcgctatcca aggagagaag caaggttgag
      301 aacatctttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt
      361 aaacgcttcg gattacgaat gaatttgatt gctggtatta tcaatcatga actaggattc
      421 tagttttgca ggaagtctat tgaggtattg agctagttta tgaaaaaatt gggtgaaaaa
      481 gtcgagtgtt ttagaaaccc acagtgtagt attctagttt caatccacta tattttgcta
      541 ctccccgtaa agtttctatt ttccctgatt tctgatataa tagaaatatt gacttcaaga
      601 gtaaggaaga gaagatgaac gcattattaa atggaatgaa tgaccgtcag gctgaggcgg
      661 tgcaaacgac agaaggtccc ttgctaatca tggcaggggc tggttctgga aagactcgtg
      721 ttttgaccca ccgtatcgct tatttgattg atgaaaagct ggtcaatcct tggaatatct
      781 tggccattac ctttaccaac aaggctgcgc gtgagatgaa agagcgtgct tatagcctca
      841 atccagcgac tcaggactgt ctgattgcga ccttccactc catgtgtgtg cgtattttgc
      901 gtcgcgatgc ggaccatatt ggctacaatc gtaattttac aattgtggat cctggtgaac
      961 agcgaacgct catgaaacgt attctcaaac agttgaactt ggatcctaaa aaatggaatg
     1021 aacgaactat tttggggacc atttccaatg ctaagaatga tttgattgat gatgttgctt
     1081 atgctgccca agctggcgat atgtatacgc aaattgtggc ccagtgttat acagcctatc
     1141 aaaaagaact tcgtcagtct gaatccgttg actttgatga tttgattatg ctgaccttgc
     1201 gtctctttga tcaaaatcct gatgttttga cctactacca gcaaaaattc caatacatcc
     1261 acgttgatga gtaccaagat accaaccacg ctcagtacca attagtcaaa ctcttggctt
     1321 cccgttttaa aaatatctgt gtggttgggg atgcggacca gtctatctac ggttggcgtg
     1381 gtgctgatat gcagaatatc ttggactttg aaaaggatta ccccaaagcc aaggttgttt
     1441 tgttggagga aaattaccgc tcaaccaaaa ccattctcca agcggccaac gaggttatta
     1501 aaaataataa aaatcgccgt cctaaaaatc tctggactca aaacgctgat ggggagcaaa
     1561 tcgtttacta tcgtgccgat gatgagctgg atgaggctgt atttgtagcc agaaccatcg
     1621 atgaacttag tcgcagtcaa aacttccttc ataaggattt tgcagttctc tatcggacta
     1681 atgcccagtc ccgtacaatt gaggaagccc tgctcaagtc taacattcct tataccatgg
     1741 ttggcggaac caaattctac agccgtaagg aaattcgcga tattattgct tatctcaacc
     1801 ttattgctaa tttgagtgac aatattagtt ttgagcgtat tatcaacgag cctaaacgtg
     1861 gaattggtct aggtacagtt gagaaaatcc gtgattttgc aaatttgcaa aatatgtcta
     1921 tgctggatgc ttctgctaat attatgttgt ctggtatcaa gggtaaggca gcccaatcta
     1981 tctgggattt tgccaatatg atgcttgatt tgcgggagca gctagaccac ttaagcatta
     2041 cagagttggt tgagtccgtc ctagaaaaaa caggttatgt cgatattctt aacacccaag
     2101 cgactctaga aagcaaggca agggttgaaa atatcgaaga gtttctttct gttacgaaga
     2161 actttgatga caccactgat gtgacagaag aggaaactgg tctggacaaa ctgagtcgtt
     2221 tcttaaatga cttggctttg attgccgaca cagattcagg tagtcaggag acatcagaag
     2281 tgaccttgat gaccctgcat gctgccaaag gtctcgaatt tccagttgtc tttttgattg
     2341 ggatggaaga aaatgtcttt ccacttagtc gtgcgactga agatccagat gaattagaag
     2401 aagagcgccg tctagcctat gtaggtatca cgcgtgcaga gaaaattctc tatctgacca
     2461 atgccaactc acgcttgctt tttggtcgta ccaattataa ccgtccgact cgttttatta
     2521 acgaaatcag ttcagacttg cttgagtatc aaggtctggc tcgtcctgca aatacaagct
     2581 ttaaggcatc atatagcagt ggtagtattt cctttggtca aggtatgagt ttggctcagg
     2641 ctcttcaaga ccgtaaacgc ggtgctgccc caaaatcaat ccagtcaagc ggtcttccat
     2701 ttggtcaatt tacagctggc gcaaaaccag catctagcga ggcaaattgg tccattggtg
     2761 acattgctct ccacaagaaa tggggagagg gaactgttct ggaagtttca ggtagcggtg
     2821 ctaggcagga attgaaaatc aatttcccag aagtaggttt gaaaaaactt ttagccagtg
     2881 tggctccaat tgagaaaaaa atctaatttt ccatccttct cacgaataat aaagtgagga
     2941 ggatttttat gtacagtatt tcattccaag aagattcact attaccaaga gaaaggctgg
     3001 ccaaggaagg agttgaagcg cttagtaacc aagagttgct agctatttta ctcaggacag
     3061 gaacacgtca agctagcgtt tttgaaattg cccaaaaagt cttgaacaat ctttcaagcc
     3121 taacggattt gaaaaaaatg accctgcagg aattgcagag tttgtctggt attgggcgtg
     3181 ttaaggccat agaattacaa gctatgattg aactggggca tcgtattcac aaacacgaga
     3241 ctcttgaaat ggaaagtatt ctcagcagtc aaaagttggc caagaagatg cagcaggaat
     3301 taggggataa aaaacaagag cacctggtgg cactctatct caatactcaa aatcaaatca
     3361 tccatcagca gaccattttt atcggttctg taactcgtag tatcgctgaa ccgcgagaga
     3421 ttcttcacta tgcaatcaag catatggcga cttctcttat cttggtccac aatcatcctt
     3481 caggagcggt agcgcctagc caaaatgatg atcatgtcac taaacttgtt aaagaagcct
     3541 gcgaactgat ggggattgtt ctcttggacc atttgattgt ctctcattct aattacttta
     3601 gttatcgtga aaagacagat ttaatctaaa gttcattaac gacatagtca aagagttttt
     3661 tatctttggg acgattttca aaaagaagtt ctggatgcca ttggacaccg agaaaggcga
     3721 catcatccgt actcatgaca gcctcaatga taccatcttt aggatcatga gccacaactt
     3781 ttaaatttgg tgctaagtcc ttgatgctct ggtggtggaa ggagttgata tgagagattt
     3841 ctccatagat ttcttggaga acggtatctg gttctgttac caagcgttga gttgtgtact
     3901 caacagaaga atcctgccaa tggtcttcga tatcttggta caaagttcca cccatggcaa
     3961 cgttaaagag ttgggtacca cggcagacag agaaaatggg ctttttctgt ttaatagctt
     4021 ccttgatgag ggccagttcg aagatatctc tttgaaggtg atagtcatcg ctatcaatgg
     4081 ttttgggttc gccatagaat tttggatcga cattttgccc acctgtcaag atgagcttgt
     4141 caatcaaact gatatagtgg caggccattt cttgatcacc aatcggtagg atgatgggaa
     4201 tccctccagc atctttaacg ccttcaacaa agccttttgc tgcgtagctc atcatgatgt
     4261 catcatctgg atgagctttt tcgtttcctg taatcccaat aactggtttt ttcataaaat
     4321 gattttcgct ttctaatcct cttttcgcat gaagtagagg agggtttgga gttcacttgt
     4381 caaatcgaca tactgaacga ccacgtcttt tggtaaatgc agatggactg gtgaaaaact
     4441 gagaattcct ttcacaccag catcaaccaa gagattagca acctcttgtg acttgacgct
     4501 gggaacagtt aggatagcag tcttcacatc agcatccttg attttatcct tgatctgaga
     4561 aatcccgtaa atgggaatcc cgtcaggagt ttgggtaccg acttcaggat ggtcgtctag
     4621 gtcaaaggcc atgataatct tcatcttgtt acgttcgtgg aagcggtagt ggagaagggc
     4681 atggcccata tttccaatac caaccagcat gacattggta atagagttgt cattgagcaa
     4741 atcggcaaaa aatgtcatta attttttgac atcatagcca aaaccacgac gaccaagttc
     4801 accaaaatag gaaaaatcac gacgtacggt cgctgaatca ataccgatag cctctgcaat
     4861 ttgcttagag ttggcacgtt caatcttttc tgcatgaaat ctcttaaaaa ttcgatagta
     4921 gagagagagt ctttttgctg tagcttttgg aatagcaaac tgtttatctt tcacaaaatc
     4981 acaacctttc tattcttcta ttttatagaa acattgtgaa aaaatcaaca aaaataagaa
     5041 aaaactaaga aaaatcttag ttttgatgta aaaaatctgc atgagataga aaacggtaga
     5101 ggtctccgac cagcccctga taaacttttt tgcccctaaa agtcagagaa gtcacataaa
     5161 gtgtatctgg taaggttaca catcctgaca aagtcaacat gagagcctca tgatcctcat
     5221 acttgagagt acgctctaca tgatagcagt ccttataggt cagttcaaac attttggctc
     5281 tatctttccg attttgtaaa gacaccacgt tctaccaagc tatccatgag gaagtagaat
     5341 ttttcctgat gaatatggtg gtcttctgat ttgaaaatat caactagacg aaggccaaac
     5401 ttgtcagtga tattgatttt agcccctgta agttccttgt taatgatgat tttgagttgg
     5461 aagccttcac cgctgtttgg cactttttcc aaaaggcgag tcagttcata gttaccaacc
     5521 ttagtttcaa aaaaggtgtt atctttgagg gtgaattttt taacagaagg gctaagagtg
     5581 taatcgtaac gacaattttt taactgaatg attttttcaa atgccatatg gctaacctcc
     5641 gataatttct tttaaggttt ttgcgagggt ttgtaggtct tcaacggtat tttgtggcga
     5701 caaactgatg cgaagggatt ccttcaagcg ttctgaattt gcgccatgca tggctttaag
     5761 aacatggctg gattggacaa cgcctgcagt acaggctgag ccagtagaga ttgaaattcc
     5821 agctaaatct agccgaagga gtaagaggtc atttttctga ccaggaaatc caatattgag
     5881 aacataaggg agatgatgtt ttcctctatt caagtaatac tgaatgccct ccagctctgc
     5941 cagaaaggca gtttctagat tttgtacatg ttgaaaatgt tcttcttgtt tttctaggtc
     6001 ttcttttagg gctgcaacca tgcctacgat ggcaggcaga ttttcagttc ctgcacgttt
     6061 tttctgttcc tgatctccgc catgtagata ggaatcaaag tccatgctag atgcgtagag
     6121 aaaaccgatt cccttaggac catggaattt gtgggcagaa gcagtgagaa aatcaatgcc
     6181 caattcttct gaatgaattg ggattttacc aatagcctga actgcatcaa catgataggc
     6241 agcagggtgt tgcttgagta tttggccaat ttcagcgatg ggaagtaggt ttcctgtctc
     6301 attattggca aacatagtag aaaccaaaat cgtatcgtca cgtaaagcct tttgaatttg
     6361 ctgggctgtg atttcttgat tttctggctg gataatggtt gcttcaaacc caaagtgttg
     6421 aaccaagtaa tcaattgttt caaggacagc atggtgctcg atggcagttg tgatgatatg
     6481 ttttccttgt tcttggtgac gaagacagta gccaatgatg gtagtattat tgccttcagt
     6541 cccaccagaa gtgaaaaaga tatgttgagg ttttgtcctt agtaactggg ctagttcctg
     6601 acgggcttct cgcaagagtt tgccagcttg acgaccatga ccatgaatac tagaaggatt
     6661 tccgtgggtt tcttgcataa ccttggtcat agctgaaata gcaactgctg acataggagt
     6721 cgttgcagca ttgtccaaat aaatcaaaga atcaccttat ttctttttat tgtaggcaaa
     6781 gagtgggctg actggttttc tttcgtgaat acggacgata gcatcaccaa ttaactcact
     6841 agcagtgatg tagcatacat ttttaggagt tttttctttt gttgctactg aatcagtcac
     6901 aagaatttct ttaatattag tattgtcaag aagctcagca gctccctcga cgaagagacc
     6961 gtggctagaa acagcataaa tttctgtagc tccttcacgt tcaacgattt tagaagcttc
     7021 agagaaggta cgtcctgtat ttaaaatatc atcaatcaag atagctttct taccttcaac
     7081 atcaccaata atataacctt cgttacgagt tgcatcgtct tgagggtagt cgataatggc
     7141 gataggagca tcaagatatt cagccaggct acgcgcacgt ttgacacctg aatttttagg
     7201 gctaacgaca acaacatctg aaccaagcaa tcctttatcg cagtaatgtt ttgcgaatag
     7261 gggaacagtg aaaagattat ccactggaat atcaaagaaa ccttgaacct gaacggcatg
     7321 caaatcaaga gtcaggatac gatcaactcc agccttaacc agcatattgg caactagttt
     7381 tgctgtaagt ggctcacgag gacaagcaat gcggtcttga cgtgcatagc caaaatatgg
     7441 aaggacaacg ttgatactgt gggcacttgc acgcacacaa gcatcgacca tgattaacaa
     7501 ttccattagg tggttgttga cagggaaact tgttgattgg atgatgtaaa catcataacc
     7561 acggacactt tcttcgatat ttacttggat ttctccgtct gaaaattgac gtgatgatag
     7621 ttttccaagt gggacaccaa cagcttgggc aattttttgt gcaatctctt ggttagagtt
     7681 gagtgcgaaa agtttcatgt tttttctatc tgacattata gaccgtcctc tgtaaacttt
     7741 ataaatccta gttatattta ccttacatat atgaactggg atttgtgtat ttttatcttt
     7801 tctattttac caaaaaatgg agattatttc agctattttt catacttttg acaaatcgaa
     7861 ccaattttga aggagctttt tggtaggaaa tctgattttt ctctaaaaat tgtcggaaat
     7921 cctgtttgcc ttgctcatga ttttccactt caagctccaa ttcgtaatct gtcatatcaa
     7981 agtatcggct ctgatccagt gccatgagac caatagctgt tttcatttca tagcgaagcg
     8041 ttgttagaca accaagaacc tgccagttct tactttggat accatgtttc gccaattcat
     8101 ccagtactag cccttgagga agttcttcct tactcagata gttctcagca tcttttagtt
     8161 gcaatttttg gttgtattcc atgtttccaa cactctgcgg gactttgagt gtcaactcag
     8221 cccagtcttc aaaggttcga atgcgcatag cgactttctt ttctcgcagt tcaaaatcaa
     8281 gcgtgtcgat gtagtaattt gtttgaagaa caggagtgac acctgtgaac tggtctttta
     8341 gacgattgta ttcatctttt ttcaatagtg ttttcaattc aatttctaaa tgtttcattt
     8401 ttcttacctt tttttatcgt tgaaagcgga tttatggtat aataagcatt gtatttattg
     8461 tatatgaatc tggagaaaaa atcaaagata tttttgacgg ataatatgag aacaagggag
     8521 aatatatgac cttagaatgg gaagaatttc tagatcctta cattcaagct gttggtgagt
     8581 taaagattaa acttcgtggt attcgtaagc aatatcgtaa gcaaaataag cattctccaa
     8641 ttgagtttgt gaccggtcga gtcaagccaa ttgagagcat caaagaaaaa atggctcgtc
     8701 gtagcattac ttatgcgacc ttggaacacg atttgcagga tattgctggc ttacgtgtga
     8761 tggttcagtt tgtagatgac gtcaaggaag tagtggatat tttgcacaag cgtcaggata
     8821 tgcgaatcat acaggagcga gattacatta ctcatagaaa agcatcaggc tatcgttcct
     8881 atcatgtggt agtagaatat acggttgata ccatcaatgg agctaagact attttggcag
     8941 aaattcaaat tcgtactttg gccatgaatt tctgggcaac gatagaacat tctctcaact
     9001 acaagtacca aggggatttc ccagatgaga ttaagaagcg actggaaatt acagctagaa
     9061 tcgcccatca gttggatgaa gaaatgggtg aaattcgtga tgatatccaa gaagcccagg
     9121 cactttttga tcctttgagt agaaaattaa atgacggtgt aggaaacagt gacgatacag
     9181 atgaagaata caggtaaacg aattgatctg atagccaata gaaaaccgca gagtcaaagg
     9241 gttttgtatg aattgcgaga tcgtttgaag agaaatcagt ttatactcaa tgataccaat
     9301 ccggatattg tcatttccat tggcggggat ggtatgctct tgtcggcctt tcataagtac
     9361 gaaaatcagc ttgataaggt ccgctttatc ggtcttcata ctggacattt gggcttctat
     9421 acagattatc gtgattttga gttggacaag ctagtgacta atttgcagct agatactggg
     9481 gcaagggttt cttaccctgt tctgaatgtg aaggtctttc ttgaaaatgg tgaagttaag
     9541 attttcagag cactcaacga agccagcatc cgcaggtctg atcgaaccat ggtggcagat
     9601 attgtaataa atggtgttcc ctttgaacgt tttcgtggag acgggctaac agtttcgaca
     9661 ccgactggta gtactgccta taacaagtct cttggcggtg ctgttttaca ccctaccatt
     9721 gaagctttgc aattaacgga aattgccagc cttaataatc gtgtttatcg aacactgggc
     9781 tcttccatta ttgtgcctaa gaaggataag attgaactta ttccaacaag aaacgattat
     9841 catactattt cggttgacaa tagcgtttat tctttccgta atattgagcg tattgagtat
     9901 caaatcgacc atcataagat tcactttgtc gcgactccta gccataccag tttctggaac
     9961 cgtgttaagg atgcctttat cggtgaggtg gatgaatgag gtttgaattt atcgcagatg
    10021 aacatgtcaa ggttaagacc tttttaaaaa agcacgaggt ttctaaggga ttgctggcta
    10081 agattaagtt tcgaggtgga gcgattctgg tcaataatca accgcaaaat gcaacgtatc
    10141 tattggacgt tggagactac gttaccattt acattcccgc tgaggaaggc tttgaaacct
    10201 tggaggctat tgagcatcca ttagatattc tctatgagga tgaccacttt ctagtcttga
    10261 ataaacccta tggagtggct tctattccta gtgtcaatca ctctaatacc attgccaatt
    10321 ttatcaaggg ttactatgtc aagcaaaatt atgaaaatca gcaggttcac attgttacca
    10381 gactagatag ggacacttct ggcttgatgc tctttgccaa gcacggttat gcccatgcac
    10441 gattagacaa gcagttgcag aagaaatcta tcgagaaacg ctactttgct ttggttaagg
    10501 gagatggaca tttggagcca gaaggggaaa ttattgctcc gattgcgcgt gatgaagatt
    10561 ccattattac cagacgagtg gctaaaggcg gaaagtatgc ccatacttca tacaagattg
    10621 tagcttctta tggaaatatt cacttggtct atattcacct gcacactggt cgaacccatc
    10681 aaatccgagt ccatttttct tatatcggtt ttcctttgct gggagatgat ttgtatggtg
    10741 gtagtctgga agatggtatt caacgtcagg ctctgcattg ccattaccta tccttttatc
    10801 atccattttt agagcaagac ttgcagttag aaagtccctt gccggatgat ttcagtaacc
    10861 ttattaccca gttatcaact aatactctat aaaaactgtc tcagagtata attattatct
    10921 taaaggagaa aactcatgga agtttttgaa agtctcaaag ccaaccttgt tggtaaaaat
    10981 gctcgtatcg ttctccctga aggggaagag cctcgtattc ttcaagcaac aaaacgctta
    11041 gtaaaagaaa cagaagtgat tcctgttttg cttggaaatc ctgaaaaaat taaaatttat
    11101 cttgaaattg aaggaatcat ggatggttat gaggtcatcg accctcaaca ttatcctcaa
    11161 tttgaagaaa tggtttctgc cttggtggag cgtcgcaagg gcaaaatgac tgaagaagat
    11221 gtacgcaagg ttttggttga agatgtcaac tactttggtg tgatgttggt ttacttgggc
    11281 ttggttgatg gaatggtgtc aggagcgatt cactcaacag cttcaacagt tcgtccagct
    11341 ctacaaatca tcaaaactcg tccaaatgta actcgtactt caggagcctt cctcatggtt
    11401 cgtggtacgg aacgttacct atttggagac tgtgccatta acatcaatcc agatgcagaa
    11461 gccttggctg aaattgccat caactcagca atcacagcta agatgtttgg catcgaacct
    11521 aaaattgcca tgttgagcta ttctactaaa ggttcagggt ttggtgaaag cgttgataag
    11581 gtcgttgaag caactaaaat tgctcacgac ttgcgtcctg accttgaaat cgatggtgag
    11641 ttgcaatttg atgcagcctt tgttcctgaa actgcagctc tgaaagctcc tggaagtacg
    11701 gtagctggtc aagcaaatgt cttcatcttc ccaggtatcg aggcaggaaa tatcggttac
    11761 aagatggctg aacgcctggg tggctttgcg gctgtaggac ctgttttgca aggtttaaac
    11821 aagccagtta atgatctttc tcgtggatgt aatgcagatg atgtttacaa gttgaccctc
    11881 atcacagcag ctcaagcagt tcatcaatag tgaaaactat aaagcgatat actatgctat
    11941 actgtagtta tgaaactatg tacgaaaagc actgccatta attcctgaga actaaattac
    12001 tgattggtgt caaaaaggaa aacttccaag cgatgatatc ctgtctatac acgacctata
    12061 gaaatctgta atatgtatgt ccgtaaaacg ataatttccc tttttgattt taaatgagta
    12121 tgaaaagaga attttttcat gagaaaacgc ataatattaa gtgtcttgac acctgatatt
    12181 atgcgttttc tacaataaaa taggctccat aatatctata gtggatttac ccactacaaa
    12241 tattatagag tcatttttgc acctttctta gcctttgtta tcgacaagca ttgttctagc
    12301 tccttgtttt tagaaaggta atttcccagc gaaagcaccc agttttttct tggtagtttc
    12361 atcgatttgt tcaagagcag agttgatggc ttgaacggtc atatcagaaa gagtctcaag
    12421 gtcctctggg tcaacgacag ctggattgaa atcaatgctg acaactttct tatcgccagt
    12481 taaggtcgct tggacaagat cttgagcaga tttgccaaca aattgcatag cagcaagttc
    12541 agcttggctt tgttccattt gtttttgaag tttttgtgct tgacgcatca tgttttgcat
    12601 gttcatcata agatttctaa agttcctttc atctcaaggg gataatcctt gttgttaaca
    12661 agtgcctgat tttctctcct aattgttatt agtagcatag ccatcaagcc caagtattga
    12721 ttttattata ccattttttt aagacatagt cttttattgg tgagagttat ttatgtcaag
    12781 gagtgattag aaataaatta aaataatgat ttttgctaag aggggctcta ttctagtttc
    12841 atggaaatga ttttactaaa gattcgtatg ctttgtttgc aaacatgtta cctctatcta
    12901 tttttg
//
LOCUS       DAVKWS010000053        12902 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKWS010000053 DAVKWS010000000
VERSION     DAVKWS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.053"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            28..753
                     /locus_tag="VW852_001668"
     CDS             28..753
                     /locus_tag="VW852_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0272776.1"
                     /translation="MFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYED
                     LEYIYRIEIERSTFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLI
                     YLKALNTLSNFYSLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLV
                     SKEKYNEAIQEALETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEAREL
                     CKIYNNPLMLMKIENYLKELDTV"
     gene            1136..1756
                     /locus_tag="VW852_001669"
     CDS             1136..1756
                     /locus_tag="VW852_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272777.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1842..2285
                     /gene="tsaE"
                     /locus_tag="VW852_001670"
     CDS             1842..2285
                     /gene="tsaE"
                     /locus_tag="VW852_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV0272778.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2275..2793
                     /locus_tag="VW852_001671"
     CDS             2275..2793
                     /locus_tag="VW852_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272779.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2801..3817
                     /locus_tag="VW852_001672"
     CDS             2801..3817
                     /locus_tag="VW852_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV0272780.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3902..5158
                     /locus_tag="VW852_001673"
     CDS             3902..5158
                     /locus_tag="VW852_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV0272781.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGNKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5213..6379
                     /gene="recA"
                     /locus_tag="VW852_001674"
     CDS             5213..6379
                     /gene="recA"
                     /locus_tag="VW852_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV0272782.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6686..8056
                     /locus_tag="VW852_001675"
     CDS             6686..8056
                     /locus_tag="VW852_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0272783.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8432..9388
                     /gene="lytA"
                     /locus_tag="VW852_001676"
     CDS             8432..9388
                     /gene="lytA"
                     /locus_tag="VW852_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0272784.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            9734..9895
                     /locus_tag="VW852_001677"
     CDS             9734..9895
                     /locus_tag="VW852_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0272785.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            9882..10088
                     /locus_tag="VW852_001678"
     CDS             9882..10088
                     /locus_tag="VW852_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272786.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            10134..10493
                     /locus_tag="VW852_001679"
     CDS             10134..10493
                     /locus_tag="VW852_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272787.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMSLG"
     gene            10622..11047
                     /locus_tag="VW852_001680"
     CDS             10622..11047
                     /locus_tag="VW852_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272788.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKV"
     gene            11455..12450
                     /locus_tag="VW852_001681"
     CDS             11455..12450
                     /locus_tag="VW852_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272789.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            12440..>12902
                     /locus_tag="VW852_001682"
     CDS             12440..>12902
                     /locus_tag="VW852_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272790.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFQFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
BASE COUNT         4085 a         2059 c         2802 g         3956 t
ORIGIN      
        1 gggcatttca ttccctccgc agaccttttg ttcaaactct cacaacgact tgaagtacca
       61 ttagattatt tttttaatga acaaattgaa attaaatcta acctctctaa tttcaagcaa
      121 ttatctgctc gactattaga tgacagaaat tatgaagatt tggaatatat ttatagaata
      181 gagattgaac gaagtacttt tctaacacta gaagaccgaa cttaccttga atggattaaa
      241 gctattattg acttctatca atatgacagt aagtgtgagg caatttcttc attggaaaat
      301 atattattaa aagtctcttc aaatactctg atttatttaa aggcgttgaa tactctatct
      361 aatttctatt ccttagtggg tcgtgaacaa gaatatgagg caaactactc tcatttaata
      421 gagttgtatc agacaaaaaa tttggaccat caagagtttt tatttggcta catcagagtt
      481 cgttacaact acgctcacta cctagtatca aaggaaaaat ataatgaagc catccaagaa
      541 gctcttgaaa cgattgaact ctgtaaacaa agacagacaa gctaccaatt ggctcctcta
      601 cttattcttg taggaaatgc tggagccaaa tttctagaca gagaacaagt caaaaattat
      661 tatatagaag caagagagtt atgtaagatt tataacaatc ctttaatgtt gatgaagata
      721 gaaaattatt tgaaggaatt agatactgtt tagttaatct tgacattata gtttttctga
      781 aacgttaaat aacctgtaag gctgatagtt agattaatac tatcagcctt atcattatgt
      841 gactctgagt ccgtctcgct ccgtgaggtg cgagacaaaa caaccacccg tttgacggat
      901 tttcatgatt tcaaatttat attcttcgaa aatctcttca aaccacgtca gctctatctg
      961 caatctcaaa acactgtttt gagcaacctg tggctagcta cctagtttga tctttgattt
     1021 tcattgagta ttagtctcta gtgtttgttt agcgtcttaa agagacattt gagaagatta
     1081 gaaacagttc tctcttttct cctagtataa tgattggtag gaagggagag agaagatgaa
     1141 atcaatgaga atcttatttt tgttagcttt aattcaaatc agtttgagta gctgtttcct
     1201 atggaaggaa tgcatcttgt cctttaaaca aagtacagct tttttcatcg gaagcatggt
     1261 tttcgtttca ggaatctgtg ctggagtaaa ttatctttat acccgtaagc aagaagtcca
     1321 tagtgtccta gccagtaaga agtcggtgaa gcttttttac agtatgttac tcttaattaa
     1381 tttgttagga gctgttcttg ttttgtcaga taacttgttc atcaaaaata cgctgcagca
     1441 agaattagtt gactttttat tgccatcctt ctttttccta tttgggctag atttgctgat
     1501 ttttttaccc ttgaaaaaat acgtgcgcga ttttcttgct atgctagaca gaaaaaagac
     1561 agtgttggtg actattttag caacacttct tttcttaaga aatccaatga ccattgtctc
     1621 acttctgatt tatactggac tgggcttgtt ttttgcagcc tatcttgtcc caaattcggt
     1681 taagaaggaa gtttcctttt atggtcatat tttccgagat cttgtattgg tcattgttac
     1741 gctcattttc ttttagagaa agcctgtttt ttatggatgt tatgatgata agtttagtag
     1801 gaaaatagac acaaaagaaa agtttttggt ataataagag tatgtacaca aaaaatgaag
     1861 aagagttgca agccttaggg gagcgtttgg gccatctatt agcaaagaat gatgttttaa
     1921 tcttaattgg agaactgggt gcaggtaaaa cgacctttac taaaggactt gcaaaaggat
     1981 tacagatttc tcaaatgatt aaaagtccta cctatactat cgtgagagag tatgaaggtc
     2041 gacttccact ttatcaccta gatgtttatc gtattgaagg agatgctgat tctatcgact
     2101 tggatgagtt tatctttggt ggcggcgtga ctgttattga gtggggaaat ctcttaggag
     2161 atgccttgcc agatgcttat ttagaattgg aaattctaaa agaagcagat ggacgccgtt
     2221 taaattttca ggcaaagggt ttgcgtgctg agaaattgtt agaggagctt caatatggag
     2281 tatgaattgc tcattaggga agcagagccc aaagatgcag ctgaattagt ggccttttta
     2341 aatcgtgtga gtttggagac agactttacc agcctagacg gagatggtat tctcttgact
     2401 agtgaggaga tggaaatatt cctcaacaag caagctagtt cggacaatca gataacctta
     2461 cttgcattct taaatggcaa aattgctggt attgtaaata ttacagctga ccagcgtaag
     2521 agagtccgtc atattggaga tctcttcatt gtgattggaa aaagatattg gaataatggc
     2581 ttgggaagtt tgttgctaga agaagcgata gagtgggcac aagcaagtgg cattctgcgt
     2641 cgtctccaac tgactgtcca aactcgtaat caagcagcag tccatcttta tcaaaagcat
     2701 ggctttgtca ttgaaggtag ccaagagcgt ggggcatata tagaagaagg gaaatttatc
     2761 gatgtttact tgatgggtaa actgatagat tagtagaaat atggttaaaa aaattattgg
     2821 aatggtgcta gctttacttt ctgtaactgt agtaggagta ggtgtttttg cttatactat
     2881 ttatcaacaa gggacagaaa ccttagctaa aacctataaa aaaatcggcg aagaaaccaa
     2941 ggttattgaa gcgactgaac ctctaaccat tctgttaatg ggagtggaca ccggaaatgc
     3001 tgaacgaact gaaacttggg tcggtagaag tgatagcatg atcttgatga cagtgaatcc
     3061 taaaacgaaa aaaacaacaa tgatgagttt agagcgggat attctgacgc gcattgaatc
     3121 agggaatggt caggctcatg aagcgaaact gaactcagca tatgcagatg gtggagcaga
     3181 gcttgctata gaaaccattc aaaaaatgat gaatatccat attgatcgct atgtgatggt
     3241 caatatgaga gggttgcaaa aactagtgga tgcagtagga ggtattacag tcaataatat
     3301 cctaggtttc ccaatttcta tcagtgacca agaagaattt aatactattt ctatcggtgt
     3361 tggggagcaa catattgggg gagaagaagc cctagtctat gcacgaatgc gttaccaaga
     3421 tcctgagggg gattatggtc gtcaaaaacg tcaacgtgaa gttattcaaa aagtcatgga
     3481 aaaagctctc agtttaaata gcgttggtca ttatcaagag attctaaaag ctttgagtga
     3541 caatatgcag accaatattg atttgtctgc aaaaagtatc cctaacttgc taggctataa
     3601 agattcattt aaaaccattg aaactcagca gttgcagggt gaaggagaga tacttcaagg
     3661 tgtttcttac cagattgttt cgagagcaca tatgttggaa atgcaaaata tactccgacg
     3721 ttctttggga caagaagaag ttactcagct tgaaaccaat gcggttttat ttgaagattt
     3781 atttggcaga gcacctgttg gtgatgaaga taattaactt ttgatacaaa taaaaaaatc
     3841 aatcgtagga aaattcctgc gattttttca aaaaaatacg aatagatagg taggaggaaa
     3901 catgaaagca gaaatcattg ctgttggaac agagattttg acaggacaga ttgtcaacac
     3961 caatgcccag tttttgtcgg aaaaactagc tgagattggg gtagatgtat attttcagac
     4021 ggctgtagga gacaatgaag ttcgtctctt gtctttgctt gagattgcca gtcaacgtag
     4081 cagtctggtg attttgacag gcggtttggg gccaactgag gacgacctaa ccaaacaaac
     4141 cctagctaaa tttttaggga aagcattagt ctttgatcct caggctcagg agaagttgga
     4201 tatctttttt gccctgcgac cagactatgc ccgaacaccg aataacgaaa gacaagctca
     4261 aattgtagaa ggagcgattc cactgccaaa cgaaacagga ctggctgttg gaggaaaatt
     4321 agaagtagac ggagtgacct atgtcgtcct tccaggtccg ccaagtgaat tgaaacccat
     4381 ggtcttaaac caacttctac ccaagttgat gacagggaac aagctgtatt cccgagttct
     4441 tcgtttcttt gggattggcg agagccagtt ggttacgatt ttggctgatt taattgataa
     4501 tcagatcgat cctaccttgg ccccttatgc caagacagga gaagtcactc tacgtctgtc
     4561 aacaaaggct agcagtcaag aagaggcgaa tcaagcgctg gatatcttgg aaaatcaaat
     4621 cttggactgc cagactttcg aaggaatttc tttacgagac ttttgctatg gttatgggga
     4681 agaaactagt ttagccagca ttgtggtaga agaactgaaa aggcaaggga aaaccatcgc
     4741 ggctgcagag agtttgacgg caggtctttt ccaagctacc gtggcgaatt tttctggagt
     4801 ttcaagtata tttgagggtg gttttgtgac ctatagcttg gaggaaaaat caaggatgtt
     4861 ggatattcct gccaagaatt tggaagaaca tggtgtggtg tctgaattta cagctcagaa
     4921 gatggctgag caggcacgaa gcaagaccca gtctgatttt ggaattagtt tgactggagt
     4981 ggcaggacca gatagcctag aaggacaccc agttgggaca gtcttcatag gcttggcgca
     5041 agatcaagga actgaggtta tcaaggttaa tattggaggc agaagccgag cagatgtacg
     5101 tcacattgcg gttatgcatg cctttaacct agttcgcaag gctttattaa gtgactaact
     5161 tttgatataa tagtagatag gtctgaggat cattagaatg taggagaata gaatggcgaa
     5221 aaaaccaaaa aaattagaag aaatttcaaa aaaatttggg gcagaacgtg aaaaggcctt
     5281 gaatgacgct cttaaattga ttgagaaaga ctttggtaaa ggatcaatca tgcgtttggg
     5341 tgaacgtgcg gagcaaaagg tgcaagtgat gagctcaggt tctttagctc ttgacattgc
     5401 ccttggctca ggtggttatc ctaagggacg tatcatcgaa atctatggcc cagagtcatc
     5461 tggtaagaca acggttgccc ttcatgcagt tgcacaagcg caaaaagaag gtgggattgc
     5521 tgcctttatc gatgcggaac atgcccttga tccagcttat gctgcggccc ttggtgtcaa
     5581 tattgacgaa ttgctcttgt ctcaaccaga ctcaggagag caaggtcttg agattgcggg
     5641 aaaattgatt gactcaggtg cagttgatct tgtcgtagtc gactcagttg ctgcccttgt
     5701 tcctcgtgcg gaaattgatg gagatatcgg agatagccat gttggtttgc aggctcgtat
     5761 gatgagccag gccatgcgta aacttggcgc ctctatcaat aaaaccaaaa caattgccat
     5821 ttttatcaac caattgcgtg aaaaagttgg agtgatgttt ggaaatccag aaacaacacc
     5881 gggcggacgt gctttgaaat tctatgcttc agtccgcttg gatgttcgtg gtaatacaca
     5941 aattaaggga actggtgatc aaaaagaaac caatgtcggt aaagaaacta agattaaggt
     6001 tgtaaaaaat aaggtagctc caccgtttaa ggaagccgta gttgaaatta tgtacggaga
     6061 aggaatttct aagactggtg agcttttgaa gattgcaagc gatttggata ttatcaaaaa
     6121 agcaggggct tggtattctt acaaagatga aaaaattggg caaggttctg agaatgctaa
     6181 gaaatacttg gcagagcacc cagaaatctt tgatgaaatt gataagcaag tccgttctaa
     6241 atttggcttg attgatggag aagaagtttc agaacaagat actgaaaaca aaaaagatga
     6301 gccaaagaaa gaagaagcag tgaatgaaga agttccgctt gacttaggcg atgaacttga
     6361 aatcgaaatt gaagaataag ctgttaaagc agtggagaaa tccgctactt tttcgatttt
     6421 tgattcaagt ttttagatta tatatagtag cttgaaataa gatatgaaca actttattag
     6481 gaaagtcaaa ttaatttcta gaaatgtttt agcagctaca gcgtactatt ccaaactcaa
     6541 ccaactataa tagatcgaaa ctagaatagt acacatctac ttctaaaaca ttgttaaaaa
     6601 tcgatttgac tgtccttatt tcattccgct atatataagt ttgctgtttc ttgtcgctcc
     6661 tctggaaagc tgatataata gctttatgaa taaaaaacga acagtggacc tgatacatgg
     6721 tccgattctt ccctcgctct taagcttcac ctttccaatc ttgctatcaa atatttttca
     6781 acagctctat aacactgctg atgtcttgat tgttggacga tttcttggtc aagaatcctt
     6841 ggctgcagta ggagcgacga cagcgatttt tgacctgatt gtaggcttta cacttggtgt
     6901 tggcaatggc atggggattg tcattgctcg ttattatggg gctcggaatt tcactaaaat
     6961 caaggaagca gtagcagcca cctggatttt aggtgctctt ttgagcattc tagttatgtt
     7021 gctgggcttt cttggcttgt atcctctctt gcaatactta gatactcctg cagaaattct
     7081 tcctcaatct tatcaatata tttctatgat tgtgacctgt gtaggtgtca gctttgctta
     7141 taatcttttt gcaggcttgt tgcggtctat tggtgacagt ctagcagccc tgggatttct
     7201 gattttctct gccttggtta atgtggttct ggatctctat tttattacgc aattgcatct
     7261 gggagttcaa tccgcaggac ttgctaccat tatttcgcaa ggtttatcag cggttctctg
     7321 cttttattat attcgtaaaa gtgtgccaga actcttgcca cagtttaaac atttcaaatg
     7381 ggacaaaagc ttgtacgcgg atctcttgga gcaaggtttg gctatgggct tgatgagttc
     7441 aattgtatct atcggcagtg tgattttaca gttttctgtt aatacatttg gtgcagtgat
     7501 tattagtgcc cagacggcag ctcgacgcat tatgaccttt gcccttcttc ctatgaccgc
     7561 tatttctgca tcaatgacga cctttgcttc tcagaatcta ggagctaagc gacctgaccg
     7621 tattgttcaa ggtcttcgaa tcggcagtcg tttaagtata tcctgggcag tttttgtttg
     7681 tattttcctc ttttttgcca gtccagcttt ggtttccttc ttggctagtt cgacagatgg
     7741 ttacttgata gaaaatggaa gtctctatct gcaaatcagt tcaacctttt atcccatttt
     7801 gagcctcttg ttgatttatc gcaattgctt gcagggcttg gggcaaaaga tccttcctct
     7861 agtttctagc tttattgaac taatcggaaa aatcgttttt gtggttttga ttattccttg
     7921 ggcaggatat aagggtgtta tcctttgtga acctcttatc tgggttgcca tgacagttca
     7981 actgtacttc tcattattcc gtcatccctt gataaaagaa ggcaaggcaa tcttggcaac
     8041 caaagtgcaa tcctagttgg atttactgaa taaaatccat ttcctctagt gaaaatcgaa
     8101 aaaacttgtg ttctcgtctt tagtttggtg ttgaaaatag tttaacagac ttttgacttc
     8161 ttttatatga tataataaag tatagtattt atgaaaagga catatagaga ctgtaaaaat
     8221 atacttttga aaagcttttt agtctggggt gttattgtag atagaatgca gaccttgtca
     8281 gtcctattta cagtgtcaaa atagtgcgtt ttgaagttct atctacaagc ctaatcgtga
     8341 ctaagattgt cttctttgta aggtagaaat aaaggagttt ctggttctgg attgtaaaaa
     8401 atgagttgtt ttaattgata aggagtagaa tatggaaatt aatgtgagta aattaagaac
     8461 agatttgcct caagtcggcg tgcaaccata taggcaagta cacgcacact caactgggaa
     8521 tccgcattca accgtacaga atgaagcgga ttatcactgg cggaaagacc cagaattagg
     8581 ttttttctcg cacattgttg ggaacggttg catcatgcag gtaggacctg ttgataatgg
     8641 tgcctgggac gttgggggcg gttggaatgc tgagacctat gcagcggttg aactgattga
     8701 aagccattca actaaagaag agttcatgac ggactaccgc ctttatatcg aactcttacg
     8761 caatctagca gatgaagcag gtttgccgaa aacgcttgat acagggagtt tagctggaat
     8821 taaaacgcac gagtattgca cgaataacca accaaacaac cactcagacc atgtggatcc
     8881 atacccttac ttggcaaaat ggggcattag ccgtgagcag tttaagcatg atattgagaa
     8941 cggcttgacg attgaaacag gctggcagaa gaatgacact ggctactggt acgtacattc
     9001 agacggctct tatccaaaag acaagtttga gaaaatcaat ggcacttggt actactttga
     9061 cagttcaggc tatatgcttg cagaccgctg gaggaagcac acagacggca actggtactg
     9121 gttcgacaac tcaggcgaaa tggctacagg ctggaagaaa atcgctgata agtggtacta
     9181 tttcaacgaa gaaggtgcca tgaagacagg ctgggtcaag tacaaggaca cttggtacta
     9241 cttagacgct aaagaaggcg ccatggtatc aaatgccttt atccagtcag cggacggaac
     9301 aggctggtac tacctcaaac cagacggaac actggcagac aagccagaat tcacagtaga
     9361 gccagatggc ttgattacag taaaataata atggaatatc tttcaaatca gaacagcgca
     9421 tattattagg tcttgaaaaa gcttaatagt atgcgttttc ttgtggagat atttccttca
     9481 atttttgcta ctatattaaa caaaaatcaa aaagcaaact agaaagttat gctcaaataa
     9541 aatctaaatt tgacaatgta aaccgagtcg gatagcttta agtactgttt tgaggttgaa
     9601 gatacgattt ttgataggaa ctcatcaatt ttagattttt aagcagcatc aataaattgc
     9661 ttccttgttt tgtcataatt tttttattta aaaaattatg acaagagtgt gctattcttt
     9721 ttatgagagg tgtatgaata tgataaatgt atgtgatgtt ggaaaaagaa taaaagaact
     9781 tagaatatct tcaaatctta ctcaagataa gattgctgag tatttgtctt tgaatcaaag
     9841 catgattgcc aaaatggaaa aaggtgaaag gaatatcacg aatggattta agtaataaag
     9901 ctccaaatct tagaaaaaag ttgggagctg atggtgaatc gccgatagat atttttaaat
     9961 tggtacaaaa gatagaaaat ttgacgctgg tattttatgg actcggaaag aatattagcg
    10021 gagtctgtta taaaggaact cagttcagtc tcattgcagt caattcagat atgccattag
    10081 gaaggtaaag attttcttta gcacatggac tgtatcatct ttattatgat gaggtgaaga
    10141 agagttcagt cagtcttatc ttgattggtg aaggagatga aactgaaaga aaagcggatc
    10201 agtttgcttc ttatttttta attttcccat cttcactgta taggatggtt gaggaaatca
    10261 gagaaaatgc caatagaact catcttgaag tagaagatat tataaaattg ggtcagtttt
    10321 atggtatcag tcataaagtt atgttatata gattgaggaa tgatggatac cttgatgcag
    10381 aagaaattaa aaatatggat attagtgtta tagagacagc ttcaagatta ggctatgata
    10441 caagtttata tcgtcctttg tcagaaagta aaaaggaaat gtcattagga taatatatta
    10501 attcaactga acaactttta gaaaataaca gaatttcgca agggaagtat gaggaactgt
    10561 tactagatgc tttcagatat gatattgtat atgggctaga tgaagagggg ggagttgtcg
    10621 tttgactagt cgtgtattta ttgatgcaga ttgtatttca gtatttttat gggttggcac
    10681 tgaacatctt ttagaaaagc tctattcggg taaaattgtt attccacaag aggtgtatga
    10741 tgaaatcaat atacctacaa ttccccattt aaaatctagg atagaccagt tggtagctaa
    10801 gggttcagct gagattgtga gcatagacat tggaactgaa gaatacgcat tatatagaga
    10861 tttaacaaga aatcatgata gtaacaagat tattggtaag ggagaagcgg cttctatttc
    10921 cttagcgaaa aagcataatg ggatattagg aagtaataac ctaagagatg ttaaatcata
    10981 tgtagaagaa ttttctttag aacatatgac aacaggagat atactgattg aagcgtttaa
    11041 agtgtaattt attactgaac aagagggcaa tcatatctgg aataatatgc ttaaaaagag
    11101 aaggaaaatt ggtgcaaatt cattttcaga ctatcttcgt ggaagtattc atcaaaatag
    11161 acaaaaataa atttggataa atcgaactca ctattcagga ggcatatgag caattcgaaa
    11221 aagaaaagtg tcaaattgag cctataggag tagaagtgaa atagtaagtc ctgcatagtg
    11281 gatgagagaa aagttctcct tgaagttttc ctgaactatc agtcgcatgt caaacgatat
    11341 gtagggtaat gtgagagggg atagcgagta gtttttggtt attttatcaa aaaacttata
    11401 ttttattata ccgaatgata aaatataata aaaatgatag aataaggaaa aaacatgaat
    11461 gtcaaaaaga taatgtcaat ttttcaatcc ttttatgttg atgtcagtat tgaggaactg
    11521 actttgactt taccaatcag ttttgtaaaa aggtttgagt atactcaaat gacttttcat
    11581 aaggaatcat ttttattgat taaagaaaag agaaggggga gtttgagttc atttgttact
    11641 caggctcgca ctatgggtga aaaagccaat atggatgttg ttttggtgtt ttcgaagtta
    11701 tcagacagtg aaaaaaagca attacttcaa gctagagttc cgtttgtaga ctttaaggga
    11761 aacctcttct tccctccatt gggactagta ctgaatgcga atgatactga agtccctaag
    11821 gaattaacac ctagcgaaca attaacgtgg attgcctttt tattgacaaa aggtcaaaaa
    11881 gtagtagatg ttgatttgct ttcacaagtc actggacttc caaactcaac aatttatagg
    11941 tgtttgagga cttttaaagc tttatattgg ttaaacaagc aaaataagct ttacacatat
    12001 acggtgtcaa agaaagaatt attcttaaaa tccgtgtcat gtttatttaa tcccatcaaa
    12061 aaacggattt tattgccaga tggcgatata aagcagataa aatctgtttc taatcttcta
    12121 tatggtggtg cttatgcttt gtcgcattca acttttttag ctgaaacgga tgaaaatatt
    12181 agctatgtca tatggcagag aaaattcaat cagttatcct tgccactttc tcagcatgtt
    12241 ttaaaaggaa agatgctaga gatatggaaa tatcgtcctt ttgtatctga gttttggaat
    12301 gattttaaaa ataatcatga taaacaattt gtagatccga tttctcttta tttgacctta
    12361 aaagatgatg atgacccacg tatagaggaa gagagtgaag cactagaaaa tatgatatta
    12421 cagtatctgg gagaagatga tgccagctaa tacgaaagtt atttttcaag aaatgtttgc
    12481 ggattttcag aactattatg ttctgattgg gggaactgct acctctatcg tattggattc
    12541 gcaaggattt aaaagtcgca caacaaaaga ttatgatatg gtcatcattg atgaagtaaa
    12601 aaataaggaa ttttatacta ccttgaatca ttttttagaa ttgggagagt atcaaggaag
    12661 tcagaaagat gagaaagcgc agctttttca atttacaaca actaatcctg agtttccttc
    12721 tatgattgaa ctatttagta tcttaccaga atatccatta aagaaggacg gtcgagaaat
    12781 tcccttatat tttgatcaag atgctagttt atcagcctta ttattggatg aagattatta
    12841 taatatattg gtgcatgtaa aagaaaccat tcaggggtat tcggtattga gtaattgtgg
    12901 tc
//
LOCUS       DAVKWS010000054        12219 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKWS010000054 DAVKWS010000000
VERSION     DAVKWS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.055"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            10..819
                     /locus_tag="VW852_001683"
     CDS             10..819
                     /locus_tag="VW852_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV0272791.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            824..1525
                     /locus_tag="VW852_001684"
     CDS             824..1525
                     /locus_tag="VW852_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0272792.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1550..2467
                     /locus_tag="VW852_001685"
     CDS             1550..2467
                     /locus_tag="VW852_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV0272793.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGKISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSIEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2495..4036
                     /locus_tag="VW852_001686"
     CDS             2495..4036
                     /locus_tag="VW852_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV0272794.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4055..4513
                     /locus_tag="VW852_001687"
     CDS             4055..4513
                     /locus_tag="VW852_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0272795.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4515..6737
                     /locus_tag="VW852_001688"
     CDS             4515..6737
                     /locus_tag="VW852_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV0272796.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6777..7886
                     /locus_tag="VW852_001689"
     CDS             6777..7886
                     /locus_tag="VW852_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0272797.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8043..8933
                     /locus_tag="VW852_001690"
     CDS             8043..8933
                     /locus_tag="VW852_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0272798.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            8958..9281
                     /locus_tag="VW852_001691"
     CDS             8958..9281
                     /locus_tag="VW852_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272799.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            9271..11262
                     /locus_tag="VW852_001692"
     CDS             9271..11262
                     /locus_tag="VW852_001692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV0272800.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            11278..11754
                     /locus_tag="VW852_001693"
     CDS             11278..11754
                     /locus_tag="VW852_001693"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV0272801.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11788..>12219
                     /locus_tag="VW852_001694"
     CDS             11788..>12219
                     /locus_tag="VW852_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272802.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPN"
BASE COUNT         4137 a         1759 c         2422 g         3901 t
ORIGIN      
        1 ggagctcata tgttactgca aaaagaacta attccaatga tagaagctaa cttaccaaat
       61 atggcatatg ctgaaaaaga cattgctaaa ttcttcttaa aacagcaacc tctgaatgat
      121 tattcatcta aagcattgtg cgaatacctt aatgtatcca aagcaacatt gactcgattt
      181 gcgaaaaaat gtggttttaa aggttttaga caattcattt tcaaatacca agagatgatt
      241 catgagaaag aaaagttggc attatataca gaggcaacag aaaaagtttt atccgactat
      301 gaggaaatgt tgagaaaaac ttacacggtt cttgatgaag ttcaacttga gcgtattgct
      361 gagatgatag aaactgctga gcgtgtatat ctctacggta aaggaagttc tgttcttgct
      421 ttacaagaaa tgaagatgag atttatgcgt ctcggagtga ttggtgaagt attatcagac
      481 gaggatatga ttttgtggag tagcttacta cttaatgaaa attgccttgt cattggagca
      541 tccatttcag gtcaaactga tattgtacta gaaggtctac aaaaagctgc agataaaggc
      601 gctaaaacag ttttaatgac tacaagaaaa tttgacgaag aagattgttt ctttgatgaa
      661 ctattgttat tagcttcgac cgatcatctc tcgtatggca atcgcatatc acctcagttt
      721 ccaatacttt taattacaga ctgcttattc tctaattatc tggaaagtcc agagagacaa
      781 tattattaca atcaaactat tatccataag gaggaataat aaaatgagcc aaatttggac
      841 taaagaaaaa tttataagcc aagttcaagg tggagtcatt gtttcttgtc aagctttacc
      901 tggtgaagcc ctttataatg aagaatttag cttgatgcct tttatggcta aagcagcttt
      961 agaggcagga gcagtgggca ttcgcgcaaa ttctgtgcgt gatattaaag caattcagaa
     1021 agtagtagat ttaccaataa ttggaattat caaaagggat tatccacctc aagaaccata
     1081 tattactgct acgatgaaag aagtagatga acttgtagaa tgcggaacaa cagtcattgc
     1141 atttgatgca actttaagac caagatatga tggcttagtt gtcagtgaat ttatcaaaaa
     1201 aataaaagaa aaatatccga atcaattgct gatggcggat gtaagtaatt tagatgaagg
     1261 tctctatgca tttaaatcag gcgttgattt tgttggtaca acattatcag gttacacaag
     1321 tacaagtgta caatcagatg agcctgattt tgaactaatg aaaaaattgg ctgattttaa
     1381 tattccggta attgccgaag gaaaaattca ttatccagaa caattaaaaa aagcttatag
     1441 tttaggtgtt accagtgtag tcattggtgg agcgattaca cgtccaaaag aaattgctca
     1501 gcgatttatt aatgtcatca aataaaaaac aatataaagg agaaatctga tgaaagattt
     1561 aactaaatac aaaggcgtta tccctgcatt ttatgcttgc tatgatgaaa atggtaaaat
     1621 tagccaagat cgtgtaaaat ctctggtaca atatttcatt gacaaaggtg taaaaggtat
     1681 ctatgtaaat ggttcttcag gtgaatgtat ttaccaaagt atagaagatc gtaaacaaat
     1741 tattgaagct gttatggaag ttgctaaagg taaattaaca gttatcaacc atattgcatg
     1801 taataacacg aaagatagta tcgaattggc aaaacattca gaaagtgttg gagtcgatgc
     1861 tattgcagct atcccaccta tttatttcaa attgccagag tattcaatcg cagcatattg
     1921 gaatgcaatg agtgaagctg cgtcaaatac agattttatt atctataata ttccacaatt
     1981 ggcaggggtt gcgttgactg gtagtttgta tgcaacaatg cgtcaaaatc ctcgtgtgat
     2041 tggagttaaa aattcttcta tgcctgtaca agatattcaa atgtttgtag ctgcaggtgg
     2101 agaagattac attgtattca atggtccaga tgaacaatat cttggtggtc gcttgatggg
     2161 agcagaagct ggtattggtg gtacttatgg cgttatgcca gatttgttct tgaaattgga
     2221 aagtttgatt caagaacgag atttagatac agctaaaaaa cttcaatatg ctatcaatga
     2281 agttatctat aagatgatat caggtaaggc aaatatgtat gctgtagcaa aagaagtttt
     2341 gcgtctaaat gaaaaacttg atttaggttc tgttcgtcaa cctttagaag cattggcaga
     2401 aggtgacttg gaagttgcaa aacaagcagc agaacttatt caacaagcac gaaaagaatt
     2461 tttataataa ataaaatcaa tatggagata tgttatgggc acaacaggat ttacaataat
     2521 tgacttaatt atcttgattg tttatttact tgcggtgttg gttgcaggta tctatttctc
     2581 taaaaaagag atgaaaggaa aagagttctt taaaggagat ggttcggttc cttggtatgt
     2641 tacttcggta tccatttttg ccacaatgct cagtccgatt tccttcttgg gactcgctgg
     2701 tagctcttat gcaggtagct ggattttatg gtttgctcaa ttagggatgg tagtagctat
     2761 tccactgaca attcgtttta tcttacctat ctttgcacgg atagacatcg atacggcata
     2821 tgattacttg gataaacgtt ttaattctaa agcacttcgt attatttcag cactcttgtt
     2881 tattatttat caattgggac gtatgtctat cattatgtac ctcccatcag ctggtttatc
     2941 agtattgaca ggaattgaca tcaatatttt gattattttg atgggtgtag ttgcaattgt
     3001 ttattcttat actggtggtc taaaatccgt attatggaca gactttattc aaggtgtgat
     3061 tctgattagt ggtgtcgttt tagctttatt tgtactgatt gctaatatta aaggtggctt
     3121 tggtgcagta gcagaaacat tagcaaacgg gaaattcctt gctgcaaatg aaaaactttt
     3181 cgatcctaac ttgctttcaa actccatctt tttaattgtg atgggttcag gctttacaat
     3241 cttgtcttcc tatgcttcat ctcaagattt ggttcaacgt tttactacaa cacaaaatat
     3301 taagaaactt aataagatgt tgttcacaaa cggtgttttg tcacttgcaa ctgcaacagt
     3361 cttttacttg attggtacag gcttgtacgt attctatcaa gtacaaaatg cagatagtgc
     3421 agctagcaat atccctcaag accaaatctt tatgtacttt attgcatacc agttaccagt
     3481 aggtatcaca ggtttgatct tggcagcgat ttatgcagca tctcaatcaa ctatttcaac
     3541 aggtttgaac tctgttgcaa cttcatggac attggatatt caagatgtca tttctaaaaa
     3601 tatgtcagac aatcgtcgta cgaaaattgc acaattcgta tctctagcag taggtttatt
     3661 ctcaattggt gtttccattg tcatggctca ctcagatatt aaatctgcat acgaatggtt
     3721 caatagtttc atgggacttg tacttggtct acttggtggt gtatttattc ttggatttgt
     3781 ttctaaaaaa gcaaataaac aaggtgctta tgcagcgctg attgtatcaa ccatcgtcat
     3841 ggtatttatt aaatacttcc ttcctccaac agctgttagc tactgggcat attcattgat
     3901 ttcaatctct gtatcagtag tttcaggtta tattgtatct gttcttactg gaaataaagt
     3961 atctgcacct aaatatacaa cgattcatga tattacagaa attaaagcgg attcaagttg
     4021 ggaagttcgt cactaataga aaagagaaaa taatatgatt atttctgaac aatctgattt
     4081 taaacgatat gcttctgtca ataaatattt ttcaaaagtc tgtgattttt tagaaaatac
     4141 aaatttgaca gatttagttg atggaaaaat tgatattgac ggggaaaatg tttttgcaaa
     4201 ttgtatgact tatctagctg atggagttcc aggagatatt tttgaaactc ataagaaata
     4261 tttagatatt catattgttg ttgaaaatac tgaaaaaatg gcagtaactt ccccagttcg
     4321 tgcccagtca cgtgtacctt ttagcgaaga gaaggatatt gctttttacg atagcaaaga
     4381 ctatcaaatt gttgaattgc ttccagggaa tatgttggtg acttttgaag aagatcttca
     4441 ccaaccaaag attcattgca atgatgaaac tgttaaaaaa cttgttatca aagttttaaa
     4501 cgaggaaaaa taaaatgaaa aaaaatatta aacaatatgt aaccttaggt actgtagtgg
     4561 tattatcagc atttgttgct aactcagttg cagctcagga gactgaaact tctgaagtat
     4621 caacaccaaa gttggtgcaa cctgttgcac caacgactcc gatttcggaa gtacaaccta
     4681 catcggataa ctcttcggaa gttactgtac aacctcgaac agttgaaact actgttaagg
     4741 atccatcttc tacagcggaa gaaactcctg tcttagaaaa aaataatgtt actttaacag
     4801 ggggcggaga aaatgttact aaagagttaa aggataaatt tactagcggt gactttactg
     4861 tagtgattaa gtacaatcag tcaagtgaga aaggcttaca agctctgttt ggaatatcta
     4921 attccaaacc cggtcaacaa aatagttatg tagatgtgtt ccttagagac aatggtgagt
     4981 tggggatgga agcgcgtgat acttcttcca ataaaaataa cctagtatcc agacctgctt
     5041 cagtttgggg taagtacaaa caagaggctg tgactaacac tgttgcagta gtagcagatt
     5101 cagtcaaaaa aacatattct ttatacgcaa atggtacaaa agtagtagaa aagaaagtgg
     5161 ataatttcct aaacatcaag gatattaaag gtattgatta ctatatgctt gggggagtga
     5221 aacgtgcagg aaaaacggcg tttggtttta acggaacact agaaaatatc aaattcttta
     5281 atagtgcatt ggatgaagaa actgttaaaa agatgacaac aaacgctgtt actggacatt
     5341 taatttatac ggctaatgat acaacaggtt ctaactattt ccgtattcca gttctgtata
     5401 cttttagcaa tggtcgggta ttttcaagca ttgacgctcg ttacggtgga actcatgatt
     5461 tcttgaataa aattaatatt gctacaagtt atagtgatga taatggtaag acatggacta
     5521 aaccaaaatt aacattggca ttcgatgatt ttgcgccagt accattagaa tggcctcgtg
     5581 aagttggtgg acgtgactta caaatcagcg gtggtgcaac ctatattgac tctgttattg
     5641 ttgaaaaaaa gaacaaacaa gtactcatgt ttgctgatgt gatgcctgct ggagtaagtt
     5701 ttagagaagc aactagaaaa gattcaggtt ataaacaaat tgatggtaat tattacctta
     5761 aattaaggaa acaaggtgat actgattaca attatactat tcgtgagaat ggtactgtat
     5821 acgacgatcg taccaacaga ccaactgaat tttcagtaga taaaaatttc ggtattaaac
     5881 aaaatggtaa ttatttgacg gtagagcaat attcggtttc atttgaaaat aataaaaaga
     5941 ctgaatatcg taatgggact aaagtccata tgaatatctt ctacaaagat gcattgttta
     6001 aagtagtgcc aacgaactat attgcatata tttctagcaa tgatcatggc gaatcttggt
     6061 ctgcaccaac tttattacct cctataatgg gacttaatcg gaatgcgcca tatttaggtc
     6121 ctggacgtgg aatcattgaa agctcaactg gacgtattct tattccgtct tacactggta
     6181 aagagtctgc gttcatttat agtgacgata atggagcatc ttggaaagtt aaagtagtgc
     6241 cacttccttc tagttggtca gcagaagcac aatttgtaga attgagtcca ggagtaattc
     6301 aagcatatat gcgtacaaat aatggtaaaa ttgcatattt aacaagtaaa gacgcaggta
     6361 ctacttggag tgcaccggaa tatttgaaat ttgtttcaaa tccaagttat ggaacacaat
     6421 tatcaatcat caattatagc caattgattg atggtaaaaa ggctgtcatt ttaagtactc
     6481 caaactccac aaatggtcgt aaacacggac aaatttggat tggtctaatt aatgatgata
     6541 atacaattga ttggcgttat catcacgacg ttgattatag taactatgga tactcatatt
     6601 caacattgac agagttacca aatcatgaaa ttggattgat gtttgaaaaa tttgattcat
     6661 ggtctcgtaa tgaacttcat atgaaaaatg ttgtaccata tataacattt aagattgaag
     6721 atctgaaaaa gaattaaagc tgaaatttga aaatatataa aaagaggata aaaattatgg
     6781 taaattacgg tattgttgga gctggatatt ttggagctga tttagctcgc tcaatgaaca
     6841 aaattgaaga tgcaaaagtg gttgcggtat ttgacccaaa tcatggagaa gaagttgctc
     6901 aagagttggg atcagatgtt tgtgcaagtt tagatgaact tgtagcacgt gaagatattg
     6961 attgtgtgat cgtagcttca cctagctacc ttcaccgtga accagttgtg aaagctgctc
     7021 aacatggcaa acacgtattt tgtgaaaagc caattgcatt gtcttatgaa gattgtaaag
     7081 ccatggttga cgcatgtaaa gaaaataatg tcatctttat ggctggtcac atcatgaact
     7141 tctttaacgg tgtacaccat gctaaagaat tgattactca aggtaaaatc ggtaaagttc
     7201 tttattgcca tgctgctcgt acaggttggg aagaacaaca accaactgta tcatggaaga
     7261 aacttcgttc tcaatctgga ggacatttgt accaccatat tcatgaatta gattgcattc
     7321 agtttatcat gggaggactt cctgaaaaag cgacaatggt aggaggcaat gtatatcata
     7381 aaggtgaaaa ctttggtgat gaagatgata tgctcattgt aaacttagaa tactctgatg
     7441 atcgttatgc tgttttggaa tatggtaatg ctttccgttg gggtgaacac tacgtcttga
     7501 ttcaaggaac tgaaggagct atcaaacttg acttgttcaa tactggcggt actcttcgtg
     7561 ttaaaggtga aggagaatca cacttcttag ttcatgaaac tcaagaggaa gatgatgatc
     7621 gtacagctat ctataccggt cgtggtatgg atggagcaat tgcgtacggt aaaccaggag
     7681 tacgttgccc attatggttg caaacatgta ttgataaaga aatggaatat ctacatgaca
     7741 tcattaaagg tggagaaatt acagaagaat ttgaaaaact tctcaatggt gtagctgctt
     7801 tagaatcaat cgctaccgct gatgcatgta ctttatcagt taaagaagat cgaaaagtaa
     7861 gtctttcaga aatcacaaat gcttaacttt tgtaaaacag aatagtaaat tcttgtcatt
     7921 atataatttc taaagttctg tgatacaact cattgaataa agaaatagag atgggactgg
     7981 gataatgccc agtcccattt tttatcaaaa agtaatgaga tcaaaaatgt gggagtgttg
     8041 aaatgaagat tataggtatc gatattggcg gaacaacaat taaggcagat ttatacgatg
     8101 agtttggaac gagtttgaat catttcaaag agatagaaac aattattgac tatgatttgg
     8161 gaacgaatca gatattaaat caggtctgtg atttaattgg tgagtatact ttaaatcatt
     8221 caattgatgg tgttgggatt tccactgctg gagttgttaa tgctaatact ggagaaatca
     8281 tctatgcagg ctatacaata ccagggtata tcggagtaaa ctttactgcc gaaatagaaa
     8341 aacgttttgg gttgtatact tttgttgaaa atgatgttaa ttgtgctgca ttaggtgaat
     8401 tgtggaaggg acaagccaaa gataagaaaa atgtagtaat ggttactatt ggaacaggta
     8461 taggaggcag tattattgtc aacggacaaa ttgttaacgg atttaactat actgctggtg
     8521 aagtaggtta tattcctgta ggtaattcgg attggcaaag taaagcctca acaaccgcat
     8581 tgattcattt atatcaaaaa aagagcttga aaactaatca aactggacgt actttcttca
     8641 ctgatttaag atctggagat aaagttgctg aagaaacttt tgaaattttt gtagaaaatc
     8701 taacaaaagg tttattaacg atttcttatc tacttaatcc agaaattctc atattaggag
     8761 gtgggattct ggatagtaag gatattttgt tacctgaaat tcaaagttct ttagctaaaa
     8821 atgcaatgga taataggttt ttacctaaaa atcttgtggc agctacatta ggaaatgaag
     8881 ctggtcgtat aggagctgta aaaaatttct tagatagaat ttctaataaa tagtatgtaa
     8941 gataaggagg tgtcacaatg actaactctg tattttcgac aatgcaagat attgagaatg
     9001 ttgcaaccga tattataaaa tcatatgata atgagattta tacttataaa gctgtttccc
     9061 aagaagaatt ggaaaaacta gaaaaaagtt atgatgaaaa aagtcacgaa gaattagttt
     9121 caatagaaag caatttagaa atgaaacaac agaaccttat tgatgaggtt aataaaacaa
     9181 tcaaggaaaa tgatgcaaat attcagtata tttcatcaag taggagagga gaatttgtag
     9241 aaaaaattat tggtagggtg gtagaaaaat atggccatta gtcagatgaa aagaatctct
     9301 ctactatttt ctaaaagtag tcttgatgat gttttaaaaa ctattcaaga actagagtca
     9361 gtgcagttcc gtgatttaaa ggttcaggat aactggtcag aagctctaga aaaagatgaa
     9421 gttgtatttc caactattca aatttttcat acttctaatt ccaatcatgg ggttattgag
     9481 ggaaatgatg ccttgactta tttgatgaat caacaacaac atttagaagc aactgtagag
     9541 aaattacaag aatacctacc gaaagaaaac acgtttaaat tattgcagca acctccgata
     9601 actacctctt atgaagaatt agagaaattt ggtaaagcta atgttgctga gggtgttctt
     9661 aaaaaagtga atcatcaaat taacagagtt catgaattag aaagacacat tcaaagtaat
     9721 aatgaggaaa tagagcgatt aataaagtgg gaaaaattag aaattgttcc tgcgaattta
     9781 gaacaatttt ctttctgtaa aggaaaagtc ggaacaattc caaggactga agataatcgc
     9841 ttatacaata gtcttttaga aaacaatatt gaagttcaag aaatattttc taatgataga
     9901 gagtacggtg ttgttgtttt ctatcagtct agttactcta tagattttga tgaatactta
     9961 tttgaaccat ttgattattc tagaaaggaa ttaccgaagc agcgagtagt agatttagat
    10021 caagaaaaca tgcagttaat aactgaaaaa gagaatatta tcgcatcgtt gcaagattca
    10081 aagaaatatt tgatagattt acaatggcaa atagactata ttttatctat ctatgctcgt
    10141 caaatctcta agaataactt tttgtgcact ccgcatctag ttgcattaga aggatggata
    10201 gaagaaactc gtattttata ttttataaaa gttatggatg agcattttgg acattctatt
    10261 tatatttatg aatcggaaac attgacggat aatcaagatg aaatacctat caaattaacg
    10321 aatcattctt taattgaacc atttgaatta ttgacagaaa tgtatgctct gcccaaatat
    10381 tatgagaaag atcctacacc tgtattagca ccattttact ttacattttt tggaatgatg
    10441 gttgctgatt taggctatgg tttactattg tttttaggaa caatgttagc attaaaaatt
    10501 tttcatctac cttcagcaac taagagattt ttaaaattct ttaatatatt aggggtagcc
    10561 gttgcaattt ggggtggaat ctatggctca ttttttggat atgagttgcc atttcatctg
    10621 atatctacaa cctctgatgt catgactata ttagtagtgt cagttgtgtt tgggtttatt
    10681 acagtatttg caggtttgtt agcttcagga ctacaaaaag taagaatgaa gaaatatgca
    10741 gaagcatata attcaggatt tgcgtggtgt gttattctgc ttggcttgtt atttattgct
    10801 gttggaatgt tgatgcctga tatgagaccg ttatttgtat tagggaaatg ggtatctatt
    10861 tttaatgctg tggggatttt gattgtttct attattcaaa ccaaaagctt gtcaggtatt
    10921 ggagcaggat tgtttaatct atataacatt tcatcttata taggtgattt agttagtttc
    10981 actcgattga tggcattagg attatctgga gcaagtatag catcagcttt caatttaatt
    11041 gttggtttgt ttccgggaat attggctaaa ctgacaattg gattagtatt attcattctt
    11101 ttacatgcga tcaatatttt tctatcgtta ctatcaggat atgttcatgg agcacgtctg
    11161 atatttgttg aattttttgg taagttttat gagggtggag gaaaaccatt tcaacctttg
    11221 aaggcttctg agaaatatat taaggttatt acaaagaatt aatggaggat atatataatg
    11281 gaacatttag caacttattt ttcaacctat ggaggagctt tcttcgctgc attgggaatt
    11341 gtattggcgg ttggattaag cggtatgggg tctgcttatg gagttggtaa ggctgggcaa
    11401 tctgccgcag ctttactgaa agaacagcct gaaaagtttg cctcagcttt gatattgcaa
    11461 ttattgcccg gaacacaagg attatatggt tttgttattg gaattttaat ttggttgcaa
    11521 ttaactccag aacttccttt agaaaaaggc gttgcttatt tctttgtagc tcttccaatt
    11581 gctattgtag gatacttttc agctaagcat caaggaaatg tagcagtagc gggaatgcaa
    11641 atcttggcta aaagaccaaa agaattcatg aagggagcaa ttttagctgc catggtagaa
    11701 acctatgcaa ttcttgcttt tgtcgtatca ttcattttga cccttcgtgt ataagaaata
    11761 aatttgcaat tcaaaggagg tgtctaaatg agcaatttag aaaacttacg agagtctgtt
    11821 attgaacaag ctcatgaaaa agggcgtatg aaattattgg attccaaaaa gaagattgat
    11881 gatgaatttg aaatgcaaaa gtcgctcatt ataaagaaaa aagaagctga acatgaacga
    11941 aagttaaaag aattgcaaca gaaatatcaa ataatttttc aacaattaaa aaataaggaa
    12001 cgccaatcaa cgttagtatc aaaacagaaa atattaaaag aactttttca atctgcttta
    12061 ctagaaatgg aatcttggag tgcagataaa gaaatggagt tcatctatcg aattctggaa
    12121 cgatattcac aacaagaggt catagtaacc tttggggaac ggactttagc taaattcaat
    12181 ttggaacaat tagagaaatt gaaattctct tttccaaat
//
LOCUS       DAVKWS010000055        12201 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKWS010000055 DAVKWS010000000
VERSION     DAVKWS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.056"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(378..1571)
                     /locus_tag="VW852_001695"
     CDS             complement(378..1571)
                     /locus_tag="VW852_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV0272803.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1709..3151)
                     /gene="gtfA"
                     /locus_tag="VW852_001696"
     CDS             complement(1709..3151)
                     /gene="gtfA"
                     /locus_tag="VW852_001696"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV0272804.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(3428..3508)
                     /locus_tag="VW852_001697"
                     /pseudo
     CDS             complement(3428..3508)
                     /locus_tag="VW852_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3509..4342)
                     /locus_tag="VW852_001698"
     CDS             complement(3509..4342)
                     /locus_tag="VW852_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0272805.1"
                     /translation="MKQDERKALIGKYILLVLGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            complement(4356..5222)
                     /locus_tag="VW852_001699"
     CDS             complement(4356..5222)
                     /locus_tag="VW852_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0272806.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5236..6495)
                     /locus_tag="VW852_001700"
     CDS             complement(5236..6495)
                     /locus_tag="VW852_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0272807.1"
                     /translation="MEWYKKIGLLVTTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6587..8749)
                     /locus_tag="VW852_001701"
     CDS             complement(6587..8749)
                     /locus_tag="VW852_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV0272808.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8857..9717
                     /locus_tag="VW852_001702"
     CDS             8857..9717
                     /locus_tag="VW852_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0272809.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVNLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9714..10649
                     /locus_tag="VW852_001703"
     CDS             9714..10649
                     /locus_tag="VW852_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV0272810.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKA
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(10775..10862)
                     /locus_tag="VW852_001704"
     tRNA            complement(10775..10862)
                     /locus_tag="VW852_001704"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10826..10828),aa:Ser,seq:gct)
     gene            complement(10869..10942)
                     /locus_tag="VW852_001705"
     tRNA            complement(10869..10942)
                     /locus_tag="VW852_001705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10906..10908),aa:Ile,seq:gat)
     gene            complement(10978..11048)
                     /locus_tag="VW852_001706"
     tRNA            complement(10978..11048)
                     /locus_tag="VW852_001706"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11014..11016),aa:Gly,seq:tcc)
     gene            complement(11068..11140)
                     /locus_tag="VW852_001707"
     tRNA            complement(11068..11140)
                     /locus_tag="VW852_001707"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11105..11107),aa:Phe,seq:gaa)
     gene            complement(11144..11217)
                     /locus_tag="VW852_001708"
     tRNA            complement(11144..11217)
                     /locus_tag="VW852_001708"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11181..11183),aa:Met,seq:cat)
     gene            complement(11228..11317)
                     /locus_tag="VW852_001709"
     tRNA            complement(11228..11317)
                     /locus_tag="VW852_001709"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11279..11281),aa:Ser,seq:tga)
     gene            complement(11328..11401)
                     /locus_tag="VW852_001710"
     tRNA            complement(11328..11401)
                     /locus_tag="VW852_001710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11365..11367),aa:Ile,seq:cat)
     gene            complement(11415..11488)
                     /locus_tag="VW852_001711"
     tRNA            complement(11415..11488)
                     /locus_tag="VW852_001711"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11452..11454),aa:Met,seq:cat)
     gene            complement(11494..11567)
                     /locus_tag="VW852_001712"
     tRNA            complement(11494..11567)
                     /locus_tag="VW852_001712"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11531..11533),aa:Pro,seq:tgg)
     gene            complement(11583..11656)
                     /locus_tag="VW852_001713"
     tRNA            complement(11583..11656)
                     /locus_tag="VW852_001713"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11620..11622),aa:Arg,seq:acg)
     gene            complement(11666..11751)
                     /locus_tag="VW852_001714"
     tRNA            complement(11666..11751)
                     /locus_tag="VW852_001714"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11715..11717),aa:Leu,seq:taa)
     gene            complement(11759..11830)
                     /locus_tag="VW852_001715"
     tRNA            complement(11759..11830)
                     /locus_tag="VW852_001715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11796..11798),aa:Gly,seq:gcc)
     gene            complement(11853..11925)
                     /locus_tag="VW852_001716"
     tRNA            complement(11853..11925)
                     /locus_tag="VW852_001716"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11890..11892),aa:Thr,seq:tgt)
     gene            complement(11944..12025)
                     /locus_tag="VW852_001717"
     tRNA            complement(11944..12025)
                     /locus_tag="VW852_001717"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11989..11991),aa:Leu,seq:tag)
     gene            complement(12031..12103)
                     /locus_tag="VW852_001718"
     tRNA            complement(12031..12103)
                     /locus_tag="VW852_001718"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12068..12070),aa:Lys,seq:ttt)
     gene            complement(12128..12200)
                     /locus_tag="VW852_001719"
     tRNA            complement(12128..12200)
                     /locus_tag="VW852_001719"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12165..12167),aa:Asp,seq:gtc)
BASE COUNT         3858 a         2704 c         2179 g         3460 t
ORIGIN      
        1 tttactatgt cttttcacac caaccttatt ccgaattcaa ttacttttgt gatttacata
       61 tatagattaa gactatcttt tatactttaa aatttctcgc taccttatcc actatatgct
      121 cctcgctatc acgtttctat tcatagccta cgatttcact attgctttct ctgacaattc
      181 ttatttcctg cgtcagactt aaaacgatct atccccagac cattttaatc cgctacctca
      241 cgatagtcag gcttggggag cgctattgta ttcaccggta gtggatccct acagaggact
      301 tacacctcag atgcacgaca tgcccatcgt ataaaaaaat ctcctaccca aggtagaaga
      361 tttcaaactt ataaaactta atccgtcatg tccgatacca acattcgatg ctccaatgga
      421 atactgcaca taactagcaa gaaaataaag cctgactgaa tccagaagag agccaagtca
      481 aaaattccgt gcacagcaac cactgtaagg aaagatagat aaaggccgat aatcggacgt
      541 ttccccgact cctgactcat atccatcatc aagcgaacag gagcaacaga agacaaaact
      601 aataaaatgg tacccacaat tccgtaactc agaatcgtat caatataaag actgtgggca
      661 tgttcatgat aaggagcatg tatccgagga taagagtgca tataggtcaa tggcccttca
      721 ccccaaaaag gattttgctt aaacaaggcc atcccagcat cccagataga aatgcgttct
      781 tccatagaag agtctaaagt acccattcga actcccaaat cactagaaaa gaggaaactc
      841 aaaccaatcg cgaagacccc aatactaagc caaaaggcct tccagttttt aatagtcgta
      901 aagagataga taattgctcc agcgataata gcaggaaagg cagttcgatt ttgagtaaag
      961 ttcaaaccaa agagattaac aaagcctgca atcacacaga atactttcaa ccaattcaac
     1021 ttggtcgttg taaacagata gaaagcaatc ataatacaga aacaacaaat aattccataa
     1081 taattaggat taaagaaggt cacttctgcc cggttctgat gccacacctg catattgggt
     1141 gaaagaaaag catagttaaa tttcttcaca atttggaaat gttctaaact ggcaaaagca
     1201 gctgacaaga cactaccaaa caagacgaac tgcaaaatca atcgaaagaa tttatgggat
     1261 aaaatcgact gatagtgcaa aaagaaaata gtaaatagaa acattcctac tgaagccaca
     1321 agacccatcc aattttgtgc aagaatggat ataacagtac tatagctaag aaaaagaagc
     1381 agcatcggat gctcccccat tttctgaaga atacttttca tgtctcctgt aaaaatcaaa
     1441 ctgataatat ataaacagag tacaactaca aaaagataaa agggtaaaaa gatacttagg
     1501 ataattccca ataaaatcag ctctttacta gacaacccct tcagcttttc aataaagcct
     1561 attgatttca aaatgaatcc tttctctcca aatcagctga ttcagataat agtaagctat
     1621 cctatattgt accacttttt tagcaatttg aaaacaaagg aaacgttttc caaaataaaa
     1681 accctatttt atccaccata tcaaggcttc aaaatgatac ttcaactcca ttctcaatta
     1741 cccgataagt ctgattttgc aaatcaattt ctactactgc tgttacggac ttatctttat
     1801 tttgacgttt gattacaatg ctgtgagctg ttggtgtctc tatctcagta gtcccttcta
     1861 gatcaaaggc ttctgaacgg ttacggaaag aaaatagatt gagaagggcc ttcacaacag
     1921 gtcgttgcac ttcttttgct atttcctcgt tgctatagta atgacgatta atatttcgac
     1981 cttctttagt ttcttctaat aatttcaagt cattcttgcc tgctaataga cccacatagt
     2041 aaacctgagg aatacctggg gcaaaagctt gaattagacg agcgagaaaa tacttgacat
     2101 catcatctcc aagcgctgaa tagtaggttg aattgatttg gtagatatct aagttgttat
     2161 actcggcact agagtactta cgtttgacat tggctccaac cttatagagt tcatttgaag
     2221 catagtcaat ctcctcatcg gtcaggatat ccttgacatc tactactcca atcccatcat
     2281 gggtatctag cgtcgtaaat tgcttcatcg ggctcatctt taaccactta gccaaacgct
     2341 ctgttctgga actgtaaaga gtataaagtg tcaccattgg aagagcaaaa tcataaacat
     2401 agtaatcatg gtctgctatt ttaaactgaa tcgaatagtg ttcatgaatc tcaggtaaaa
     2461 gctctgtccc atactcggca gcgatatctc gaactttgtc caataaatcc caaatatctg
     2521 gttccacaaa gaaatcatta gtatccaatt tcttcactgc ataagcaaag gcatctagac
     2581 gaatcaaatc acacccatta cttgccaagt gctgaatggt cttacggata aattccatag
     2641 ttacttcttt ggtcacatca agatcaatct gctcctcacc aaaggtattc cacaaatgtt
     2701 ccactgaacc atcttcaaac acaatctctt gctttggtgc acgatcctta cgcttgtaaa
     2761 ttaaatctac atcagactgt gtcggacggt tttctggcca aaacttatcc cagtttaaaa
     2821 agagatcttt aaattcactg gcttcatgtt tttcttgata gtccttataa tacttggatt
     2881 gacgagaaat atgattaatc ataaaatcaa acataagata atatctctca cctaaacgct
     2941 taacatcctc ccaatcacca aaagctgagt ccacttcgtc gtagtcaact ggcgcaaatc
     3001 cacgatcacc tgttgatggg aaaaatggta aaaggtgaac tcctccaata gcatctccaa
     3061 aatgctcttc caaattatca tataagtctt taagattatt tccaaggcta tcagaatagg
     3121 taatcaacat ggttttattt tgaattggca tcattactct cctttttcta attgaagcca
     3181 agtctcatat gatctggctt cgtaaatcaa attcatttta aatctctatt tatcatcaaa
     3241 ctcgtactaa tatagactgt gataaacaaa gtactacttt cttgttttct acatagaatt
     3301 atcaacaagc tgaactcttc ctctgtgtca aagactatag attccatgag ctcttcttat
     3361 actattcgaa aatctcttca aaccacgtca gcgtcgcctt accgtaggta tggttactga
     3421 cttcgtcagt ttcatccaca acctcaaaac agtgttttga gcaacctgcg gctagcttcc
     3481 tagtttgctc tttgattttc attgagtatt acttcactgc cccgttgctt attcctgcga
     3541 tgatatggcg ttggaagaag agatagacaa tggtgatact gataatgccg accacgtaag
     3601 aggcaaagct tggtccgtag tcgttgaaat attggcctgc gtagttgtat tggaacaaag
     3661 gcagagtcca cattttggaa tcccggttca agacaaggag tggcaacatg aagtcattcc
     3721 agaaccaaag ggcattgatg atcatggttg tcgcatgcat cggtttcatc attgggaaga
     3781 tgatgcggaa ataggttgta aattgattag ccccatcgat ctctgctgct tcatccagac
     3841 tttctggaat cgagattttg atatagccaa catagagaaa gagggtctgt ggaatcgcat
     3901 aggtcaagta gagcaagatc aaaccaaagg tattagccaa accgagttta ctcatcataa
     3961 ccgtaatcgg aatcatgatg acttggaaag gtacgaatat tccgaggatt aagagggtat
     4021 acatgatggt aaaggctttt cttttactca tattgcgagc gatggagtag gctgccatag
     4081 ggataaagat cattactgca agtaaagaca agacagtgat gacgacagag ttccaataat
     4141 agcctccaat cccatcagct aagagacggc taaagttgtc ccatgtgaag ttggttggaa
     4201 agccaaagaa attatctaca atatccttag tgggtttgaa ggaactaaag agggtagcaa
     4261 ggagcggcac taaaatcaga accgatccta gaaccaatag aatgtatttg ccaatcaggg
     4321 cttttctttc atcttgtttc atcatgcttc tcctcttaaa tttcaaattt cttagatact
     4381 ctcaattgga tgatcgaaat cactacaatt aagaggaaca agattacggc aatggcattg
     4441 gcataaccga attggttgtt tttaaaggca tagttataaa ccaagagccc aagtgaggtt
     4501 gtggcattgt ttggaccacc accggtcatg gcaaagactt ggtcaaaggc agtcagccca
     4561 ccttttaggg ctaggataaa gaccatagag acacttggta gcaagtaagg caattcaatg
     4621 ttccagaaaa cttgcttgct agtcgcacca tcaatccttg ctgcctctgt aatctcagtt
     4681 ggaatagatt gcaaaccagc taggaagatg atgatgggca tagccacccc ttgccaaaga
     4741 aggacaaagg cagccgcaaa gattgctccc cacttagtcc ctaaaagact ggtttggaga
     4801 aattcaatat gaagggcatt cccaatcgct ggaagaccgt agttgaagac ttgcttgaag
     4861 atcaaagcca ctgtcaaacc agataaaaca gctgggaaga agaaccaagc acggaagaag
     4921 gtttggcctt tgattttaga attcaagaca cgcgcaatga agatcccgag tgcaatctca
     4981 ccaaccacca tggcaatcgt aatgattgcg gtaaagccaa tcgcattcat gaattttgga
     5041 tccatgaaga ggagcttaaa gttgtttaag ccaacaaatt tgtagttata agtcaatcct
     5101 gtccagttgg taaaactgta aaaggctcct tgaaacatcg gcacatagaa gaaaattgct
     5161 tgtaacaaga gggggatgac cacaaaagcc catgcccaat atttttgtaa tacttttttc
     5221 atagtctctc tactcctaat ccacatccgc tttcatcggg ttaaagaagg cattcaaatc
     5281 attgaccatg ccttgtttat caccggtcaa gacatagttc atggtcaagg tatggaagtc
     5341 tgcttcactg gtccagtatt gttgcaacca gaccaagtga cgatccgtaa aggcatattc
     5401 ggtcatacca gcaagcggtg aatcttctcc tgcttgtttg accccttcga tcgctgttgg
     5461 agatccgtcc acatcgtagt atttttgcat gacttctgga cgggtcatat attccacaaa
     5521 ggcattggct tcttttggat gtttggtggt ggctgagata gaccatgcca agtctcccgc
     5581 accaacggtt aagctttgtc ctttttcttt tcctggaatc atgaaggtcc caatcttaaa
     5641 gttcggtttt tgttcattaa tcgctgtgat cgcccaagac ccatttggtg tcatgaggac
     5701 atccccacgt gcgaaggctc cgataacatc ggtatagcca gcaccttccc agttcttttg
     5761 cttagagcca ttgatgcgaa ggatgtccat gaccttgata tcatctttca taatcggatc
     5821 cgacaattta atggcatttg gttgagaata acgaaggtat tgatttgctt cttttcctcc
     5881 acctgttgct gtcgcaaagg ctaattgatt gtaaccattg agtgtccaag catctgcacc
     5941 tgcaattcca aatggtgttt gtcctttagc aacgatatct ttgactaact gttcaaattc
     6001 atcccaggtt tcaggaacct tcaagcccag ttcttcgaat ttatctttgt tgtagtaaat
     6061 tccataagca ttagctgtaa aaggaacgtt gtaaactttt tcgtttacag catatttttc
     6121 agcgtagcca tttttcacgc gtttcaggta gtctttgttg ctcaaatctt caaagacacc
     6181 tgcttttgcc cattcttgca gttcgatgga ctgtgggtaa atattgacca catcaggcac
     6241 atctcctgcg agaacgcgtg tcttcaatac ttcaccagca tttggtacat tgacgacttt
     6301 gaccttgatc ttagggtttt ccttctcaaa atcacgagtg atttcttcca aggttttggt
     6361 catttctttt ttctggttga aatactcgat ggtcactgtg ccatccgcag atttaccata
     6421 gttggagcaa gcgccgagcc caaccaaagc taaacctgta gttacaagaa gtccgatttt
     6481 tttataccat tccattagaa agcctccttt ataaatttat acacccttat tgaactgcac
     6541 cccaaaagtt agacagaata aatctaactt ttggggtcag tacatatcat agttttctaa
     6601 aaatatactg tctactcaaa aaatctcctt gggataagat aacagttaag cccgcataca
     6661 ttagttctgc acctgagtaa acttcgccat tttcctgtaa tttatatagt ccctcttcat
     6721 ccaaatcttt taattttaaa gttgtttcca tggtctctac aacagataaa acgcgaacgt
     6781 aggttacaat cgtttgattt ccgtaattaa attgtacagc tgcttcattg gatgcagtat
     6841 caggattaat tagtctatac tgctgtccta actgaactac tggtcgtaat tctttataca
     6901 agttcacctg attagcaatc gtagctttct cttcatctga taaatttgtc aaatcaagct
     6961 catagcccaa atttcccatc attgctacat ggccacgtgt ttctaatggt gtcattcgtc
     7021 ccatctgatg attcggtact gctgacacat gagcccccat agaaatggtt ggatagagat
     7081 aggatgaacc gtattgaatt ggtaaacgtg caatggcatc agtattatca ctagcccaga
     7141 cttgtgggaa atagcgtatc ataccaagat catttcgtcc accaccacca gagcaggact
     7201 caaagagaat atggctgtgc ttctctgtca gataagaaac gagttcgtaa agccccagca
     7261 tgtactgatg agattgcatc tgtgtctcta gataagttaa tccattccct agcttagtga
     7321 tattgcggtt catatcccat ttaatgtaat caatctcatg ataagatagg agttgatcta
     7381 agacactttt caagtattct actacctgag gattggcaag attaagtact aattgattcc
     7441 gagaataagt atgctcataa tcaggaacct gaatagccca gtcaggatgt tgacgataca
     7501 aatcactatc tacagaaatc atttcgggtt ctaaccaaag tccaaactgc aaacctcttt
     7561 catggatagc tgaaatcaga ctttctagac ttccacccag tttttcctca ttaacaaccc
     7621 aatcacctaa agcacgatta tcatcaaaac gattgccaaa ccaaccatca tctaatacaa
     7681 aaagttcaat gccaactttc ttagcttcat ctgctaactc taacagtttt tctctctgaa
     7741 agtcaaagta agtagcttcc cagttattga ttagaattgg acgttctttt ttagaaaatt
     7801 cacttagaat aatgtgcttc agtacaaaat tctgactttc atgactaata ccagttaatc
     7861 cctgatctga atgagtcact aaagctaccg gtgtttcaaa gtattcctca ggggctaact
     7921 tccaagaaaa gttttctgga ttaatgccaa tagccacccg aacttcattc aattgatttt
     7981 tttgaacaaa agcttcaaag ttgccactat acattagttg aatagcaaac acattcccag
     8041 catcctctgt gactccttgt tcgcatagta gaagagctgg tgtttgagca tgaccagaag
     8101 cacctcggtt tgagctaatc gaaaagattc cttgttctac ctgttgacgt ctaacagtct
     8161 tttcacgagc ataagcaccc tgcagagtta ctatttcgta atctgcagct ggaaaatcag
     8221 ccataaaaga aaaatcttta tggatgacaa cttcctgatt actattatta tctaatttac
     8281 tgtagctagc aatagtcgca tcattattaa aagtagtata atacaaagtc agactaagtt
     8341 gagccttaga atcttctaac attaagacaa gagtctctgt atcgtccatg ctatgtggag
     8401 aaggtaagcc ctgtggacca ttctgacctt ttaaaatctt tgcttctaca aatcgaaagt
     8461 ctgttacttc agttacacta tgctgaacct gtatggttgg tttcctaaaa tctcctaagc
     8521 catgttgtcc aaaaatctgt cgctgagtat ctaaactaaa ggttcgatta gtagccgttg
     8581 gatttcctga aaaggcatgg tctcgttcat aaacactatt ggaaccttta tagttcttaa
     8641 tagtctttcc taaatgtttc aaaagtaagt agccatttcg attttcaata atcaaactta
     8701 gatttttact ctcaacataa aatagattat tctctatcct aactcccatt tacttcacct
     8761 catcacttta ttgattatat tttatcacct gaaatcgctt tctaaaatag aaaaatgtct
     8821 caagaatatg gtaaaatgtt aggtaggagg tagcacatgt tagttttttc agaataccag
     8881 actggaacaa tcgaccttgc cctaagcttt tatggatatg aggaatgcac acctaattac
     8941 tcttttggtc cagccattcg tgatacatac gtcctacatt acattactaa aggacaagga
     9001 aaatttcatt acaagggtaa aattgttaat ttaaaagaag gagatttctt tctattaaaa
     9061 ccagaggaac taacctttta tcaagcagat agtaaagaac cttgggccta ctactggtta
     9121 ggaatcactg gagggaaatc ccctgattat tttgctcttt ctcaaatttc tgatcaatcc
     9181 tatctcatcc aatctgaaac ttgtcatacc cagactactg caaaactcat ctcagacatt
     9241 gtccgcttcg ctcagattac aaaatcaagt gaattagctc aactccatat catgggacaa
     9301 cttcatgaac tgatgtttca tctgggaact attgctccca atcagaaaaa aaagaatatt
     9361 tcatcaaccc accaactcta tcttgaatgc aaacgattaa ttgatagcca ctatcctcaa
     9421 tcacttacaa ttcaagattt agcaaaagaa ctatccgttc acagaagcta cttatcaagc
     9481 gtattcaaag aatttaatac cttatcaccc aaagaatacc tactctacgt tcgaatgcac
     9541 cgagctagac aacttctcga aaatacccaa gagtccatca aggtaattgc atactcggta
     9601 ggtttttcag atccactcca tttttcgaaa gcttataaac aatactttaa tcagactcca
     9661 agtcatacaa gaaaagaata ctctcaatac caactagtga gaaaggcaac attatgaaat
     9721 cctaccaagc tgtctaccaa atcctatcta aagaaaccga ctatatcagc ggagaaaaaa
     9781 tcgcagaaaa actatcccta agccgaacat caatttggaa agctatcaag cgactagaac
     9841 aagaaggcat tgaaattgat agtatcaaaa atagaggata taaactaatg aatggtgacc
     9901 ttattcttcc agagattcta gaagaaaatc ttccaattaa agtcagcttt aaacccgaaa
     9961 caaaatcaac acaactagat gcaaaagaag caattgattt aggccatgaa gcaaataccc
    10021 tctatctagc ttcctatcaa acagcaggcc gaggccgttt tcaacgttcc ttctactcac
    10081 cacaaggtgg tatttatatg acactccatc ttaaaccaaa tctcccctat gacaaattac
    10141 catcctacac actacttgta gctggagctg tctacaaagc cattaagaac ctaactttaa
    10201 tagatgtcga cataaaatgg gtcaatgata tctatctaaa caatcataaa attggaggaa
    10261 tccttactga agcaatgacc tctgtagaaa ctggcttagt cacagatatc attattggag
    10321 taggtatcaa tttcactatt aaagacttcc ctcaggaatt aaaagcaaaa gctgccagct
    10381 tatttaaagc tacagctcct ataacaagga atgaattgat catagaaatc tggcgtgctt
    10441 tcttcgaaac accagcagaa gagctattat acctatacaa aaaacagtca ttcattctag
    10501 gaaaagaagt cactttcaca ctagagcaaa aagactacaa gggacttgct aaagacatct
    10561 cagaaaatgg aaaactttta attcaatgtg ataacggaaa agaaatctgg ctaaatagtg
    10621 gcgaaatttc tctcaatagt tggaagtaaa ataacacaat tataatataa acgatataaa
    10681 aataacttca gattagtaat tcaattaagt tttacggatc tgaagcttta ttgtctctaa
    10741 aaataaaaaa gagagttaca aactctcatt aaaacggaga ataagggatt cgaacccttg
    10801 cgccagttac ccgacctaac gatttagcaa accgtcctct tcagcctctt gagtaattct
    10861 ccaattaatg ggcacgagtg gactcgaacc accgacctca cgcttatcag gcgtgcgctc
    10921 taaccacctg agctacgcgc ccaagttaaa aaacttggta atttgaacaa agttcaaagc
    10981 gggtgacgag aatcgaactc gcgacaacag cttggaaggc tgtagtttta ccactaaact
    11041 acacccgcat aaatactatc aataaaatgg cgcgagacgg aatcgaaccg ccgacacatg
    11101 gagcttcaat ccattgctct accaactgag ctaccgagcc ttattgcggg agcaggattt
    11161 gaacctacga ccttcgggtt atgagcccga cgagctaccg agctgctcca tcccgcgtta
    11221 atataaaagg aggatgtggg attcgaaccc acgcacgctt ttacacgcct gacggttttc
    11281 aagaccgttc ccttcagccg gacttgggta atcctccaat attcaaatgg accttgtagg
    11341 acttgaacct acgaccactc ggttatgagc cgagagctct aaccagctga gctaaaggtc
    11401 cgacaagatc attatagcgg cgaaggggat cgaacccccg acctcccggg tatgaaccgg
    11461 acgctctagc cagctgagct acaccgccat gaatcgggaa gacaggattc gaacctgcga
    11521 caccttggtc ccaaaccaag tactctacca agctgagcta cttcccgagt taaatagaaa
    11581 aatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt actctatcca
    11641 gttgagctaa gggtgctcca tattatgccg aggaccggaa tcgaaccggt acgatcgtta
    11701 ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc gccaccccgg cctctctaag
    11761 cgaacgacgg gattcgaacc cgcgaccccc accttggcaa ggtggtgttc taccactgaa
    11821 ctacgttcgc actgttttct tctatctaaa aatgccggct acatgacttg aacacgcgac
    11881 cctctgatta caaatcagat gctctaccaa ctgagctaag ccggctcatt tgttatatct
    11941 taatgcgggt taagggactt gaacccccac gccgttaagc gccagatcct aaatctggtg
    12001 cgtctgccaa ttccgccaaa cccgcatata tgacccgtac tgggctcgaa ccagtgaccc
    12061 attgattaaa agtcaattgc tctaccaact gagctaacga gtctaaaata acttgcgtta
    12121 ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg ccgtgacagg gcgacgtgat
    12181 aaccgctaca ctacgggacc t
//
LOCUS       DAVKWS010000056        11837 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKWS010000056 DAVKWS010000000
VERSION     DAVKWS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.057"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            116..874
                     /locus_tag="VW852_001720"
     CDS             116..874
                     /locus_tag="VW852_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0272811.1"
                     /translation="MALVEFKNVEKYYGDYHALRDINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            885..1679
                     /locus_tag="VW852_001721"
     CDS             885..1679
                     /locus_tag="VW852_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0272812.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSD
                     YGIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1692..2369
                     /locus_tag="VW852_001722"
     CDS             1692..2369
                     /locus_tag="VW852_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0272813.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2379..3038
                     /locus_tag="VW852_001723"
     CDS             2379..3038
                     /locus_tag="VW852_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0272814.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3333..4523
                     /locus_tag="VW852_001724"
     CDS             3333..4523
                     /locus_tag="VW852_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV0272815.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4844..5725)
                     /locus_tag="VW852_001725"
     CDS             complement(4844..5725)
                     /locus_tag="VW852_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV0272816.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5866..7194)
                     /gene="vncS"
                     /locus_tag="VW852_001726"
     CDS             complement(5866..7194)
                     /gene="vncS"
                     /locus_tag="VW852_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV0272817.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYAWTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     NEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(7191..7847)
                     /gene="vncR"
                     /locus_tag="VW852_001727"
     CDS             complement(7191..7847)
                     /gene="vncR"
                     /locus_tag="VW852_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV0272818.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIQKLRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7896..7979)
                     /gene="pep27"
                     /locus_tag="VW852_001728"
     CDS             complement(7896..7979)
                     /gene="pep27"
                     /locus_tag="VW852_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV0272819.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(8159..9538)
                     /gene="vex3"
                     /locus_tag="VW852_001729"
     CDS             complement(8159..9538)
                     /gene="vex3"
                     /locus_tag="VW852_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV0272820.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9590..10237)
                     /gene="vex2"
                     /locus_tag="VW852_001730"
     CDS             complement(9590..10237)
                     /gene="vex2"
                     /locus_tag="VW852_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV0272821.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10250..11527)
                     /locus_tag="VW852_001731"
     CDS             complement(10250..11527)
                     /locus_tag="VW852_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0272822.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3333 a         2657 c         2237 g         3610 t
ORIGIN      
        1 gatcgatttt tcctgttctt atttcactat agtgaatttt ttcagatatg atttgacaag
       61 aacattcttt tattgtagaa tcatgtcata aaatctaaca caagggagag ctctcatggc
      121 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccatgcac tccgcgacat
      181 caatctccgt tttgaaaaag gacaagtcgt tgtcctactc ggaccatctg gttctgggaa
      241 atccactctt atccgtacca tcaatggttt ggaggctgtc gacaagggaa gccttctagt
      301 caatggccac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
      361 cggcatggtt ttccagcatt ttaacctcta ccctcacaaa acagtgttag aaaacgtaac
      421 actcgcaccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa ccgcccaaaa
      481 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
      541 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
      601 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
      661 gcagaaactg gcgcatgatg ggatgaacat gatcatcgtt acccacgaaa tgggctttgc
      721 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag tagatacgac
      781 agatgtcgat aacttttttg acaatccaag cgaacctcgt gcccaacaat tcctcagcaa
      841 aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa aaaaaattct
      901 ttttatcagc attattgatt agccttttcg gccttgctgc tgccaaacca gtccaggctg
      961 atactagtat cgcagacatt caaaaaagag gcgaactggt tgtcggtgtc aaacaagacg
     1021 ttcccaattt tggttacaaa gatcccaaga ccggtactta ttctggtatc gaaaccgact
     1081 tggccaagat ggtagctgat gaactcaagg tcaagattcg ctatgtgccg gttacagcac
     1141 aaacccgcgg cccccttcta gacaatgaac aggtcgatat ggatatcgcg acctttacca
     1201 tcacggacga acgcaaaaaa ctctacaact ttaccagtcc ctactacaca gacgcttctg
     1261 gatttttggt caataaatct gccaaaatca aaaagattga ggacctaaac ggcaaaacca
     1321 tcggagtcgc ccaaggttct atcacccaac gcctaattac tgaactgggt aaaaagaaag
     1381 gtctgaagtt taaattcgtc gaacttggtt cctacccaga attgattact tcccttcacg
     1441 ctcatcgtat cgatgccttt tccgttgacc gctctattct gtctggctac actagtaaac
     1501 gcacagaact actagatgat agtttcaagc catctgacta cggtattgtt accaagaaat
     1561 caaatacaga gctcaacgac tatcttgata acttggttac taaatggagc aaggatggta
     1621 gtttgcagaa actttatgac cgttacaagc tcaaaccatc tagccatact gcagattaag
     1681 gaggacaccc catgacagat ttatcatctt ggacagccta ttttcaggat tttggacaat
     1741 ttttcaatgg tttcctcttt actcttgccc tagcgattgg atcctttatc ctcgccatgg
     1801 tcttaggaat cttctttggt gccatgtcaa ccagtaaacg tccaatttta cgcattttag
     1861 ctcacatctt tgtggaattt taccaaaaca ctcccctctt ggtgcagttt gttattgttt
     1921 tttatggcct acctcttatc agtgaccata ctatcatgat tccaatttat tggacagctg
     1981 ttctctgcgt gggactctat cacggcgctt atatcgctga ggttattcgt tcagggattc
     2041 agtctattcc tagtggtcag atggaggccg ccttgtcgca aggttttacc tatatcagtg
     2101 ccatgcgctt gattatcttg cctcagacct tccgcatcat tctccctcca ttgaccaacc
     2161 aaattgttaa cctcatcaag aacacctcta ctgtagctat catctctgga gtagacttga
     2221 tgtttgtgac taagtcttgg tcggctctca acggaaacta tatcccagcc tttttaggtg
     2281 ctgctcttct ctactttgct ctatgcttcc ctgttgccca gtttggtcgc aagatggagc
     2341 aagccaacaa aaaagcctat tcactttagg aggttactat ggaatctatt ttagaagttt
     2401 taaccccaga taacctagtc tttatcttta aaggatttgg cttgaccctc tatatttctc
     2461 tgattgccat catcctctct actatcatcg gtacggtgct agctgtcatg agaaatggca
     2521 aaaatcctgt cttacgcatt atttccagta tttatatcga gtttgtgcgc aacgttccca
     2581 accttctctg gatttttact atctttttgg tgttcaaaat gaaatccaca ccagcaggta
     2641 ttacagcctt tactctcttt acatcagcag ccttggctga gattattcga ggcggtctca
     2701 atgccgtaga caagggacag tacgaagcag gaatgtcaca aggcttcacc tcagcccaaa
     2761 tcctctacta catcattctc ccacaagcca tccgcaaaat gctaccagcc atcatttctc
     2821 agtttgttac cgtgattaag gataccagtc tcctctactc tgttatcgcc ctacaagaac
     2881 tctttggagc cagccaaatt ctcatgggcc gttatttcga accagagcag gtcttcagtc
     2941 tttacatcct gattgccctc atctacttca gctttaacct agcaatttct agcctgtctc
     3001 atatgctagc caaacgttgg caacaagctg cagaataata ctcttcgaaa atctcttcaa
     3061 accacgtcag cttcaccttg ccgtatatat gttactgact tcgtcagttc tatccacaac
     3121 ctcaaaacgg tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat
     3181 tgagtataaa cagccactct ccagttcaat tggagagttt tttgatgaga gtagatattt
     3241 atagacctca atttgacacc atacattttc tatgataaaa tgtaacaaat tcatttacaa
     3301 caaatcgaaa tctaaatgaa atggaatttt ctatgaaatc agtcaaagga ctactcttta
     3361 tcatagctag ttttatcttg actcttttga cttggatgaa cacttctccc caattcatga
     3421 ttccaggact agctttaaca agcctatctc tgacttttat cctagccact cgtctcccac
     3481 tactagaaag ctggtttcac ggtttggaga aggtctacac cgtccacaaa ttcacagcct
     3541 ttctctccat catcctacta atctttcata actttagtat gggcggtttg tggggctctc
     3601 gcttagctgc tcagtttggt aatcttgcca tctatatctt tgccagcatc atccttgtcg
     3661 cctatttagg caaatacatc caatacgaag cttggcgatg gattcaccgt ctggtttacc
     3721 tagcctatat tttaggactc tttcacatct acatgataat gggcaatcgt ctccttacat
     3781 ttaatcttct aagttttctt gttggtagct atgccctttt aggcttacta gctggttttt
     3841 atatcatttt tctatatcaa aagatttcct tcccctatct aggaaaaatt acccatctca
     3901 aacgcttaaa tcacgatact agagaaattc aaatccatct tagcagacct ttcaactatc
     3961 aatcaggaca atttgccttt ctaaagattt tccaagaagg ctttgaaagt gctccgcatc
     4021 ccttttctat ctcaggaggt catggtcaaa ctctttactt tactgttaaa aattcaggcg
     4081 accataccaa gaatatctat gataatcttc aagccggcag caaagtaacc ctagacagag
     4141 cttacggaca catgatcata gaagaaggac gagaaaatca ggtttggatt gctggaggta
     4201 ttgggatcac ccccttcatc tcttacatcc gtgaacatcc tattttagat aaacaggttc
     4261 acttctacta tagcttccgt ggagatgaaa atgcagtcta cctagattta ctccgtaact
     4321 atgctcagaa aaatcctaat tttgaactcc atctaatcga cagtacgaaa gacggctatc
     4381 ttaattttga acaaaaagaa gtgcccgaac atgcaaccgt ctatatgtgt ggtcctattt
     4441 ctatgatgaa ggcacttgcc aaacagatta agaaacaaaa tccaaaaaca gagcttattt
     4501 acgaaggatt caagttcaaa taagtaatca tactctcctg ggatttccca ggagagtttt
     4561 ttatagccaa atgaaataag accggaacga tggaattttg gcattcaaat caattgataa
     4621 gaatatttta tagtggagtg aaataggata cgaacaaatt gattaggaaa atcaaatgaa
     4681 tttatagaaa tcttttagca gttatgttgt cctattctag tttcaaaacg ctatagaagc
     4741 agcattgtgc tagtctagat tcagtttact atactaaaac gagtagcttg aaatcaaaaa
     4801 acccaccctc acaggcaggt tttatctgta ttattcagct agattatgct ttaccttctg
     4861 aaccgaatac gtcgatacgt tcttcaaccg atgcttggat agcttttaca ccgtcagcca
     4921 agaatttacg tgggtcgaag agttttttct tgtcgtattc tgcttcgttt gcttcgtagt
     4981 cacgagcaaa tttacgagtt gcgttagcga atgcgatttg gcattctgtg ttaacgttaa
     5041 ctttggcaac accaagtttg atagctgctt ggatttgctc atcaggaata cctgatccac
     5101 cgtgcaatac gattgggaat cctggaagag cttctgtcaa tttttgcaag tggtcaaggt
     5161 caagaccttc ccagtttact gggtaaggac cgtggatgtt accgatacca gctgccaaga
     5221 agtcgatacc agtttcaacc attgctttag cgtcttcgat tggagccaat tcacctttac
     5281 cgatgattcc atcttcttca ccaccgatag taccaacttc agcttctact gagatacctt
     5341 tagcgtgtgc tttttcaaca acttctttag ccaatttaag attttcttca actggaaggt
     5401 gtgaaccgtc aaacatgatt gaagtataac caacttcgat acactcaagt gcatcttcgt
     5461 agtgaccgtg gtcaaggtgg atagctactg gtacagtgat acccattgat tcaacaaggt
     5521 tagcgatcaa gttgcgagca actttgtaac cacccatgta tttagcagca cccattgaag
     5581 tttggatcaa aactggagct tttttagctt ctgctgcgcg caagatagct tgagtccact
     5641 caaggttgtt tgtgttaaat ccaccaactg cataaccgtt gtcacgggct gcttggacaa
     5701 atttttctgc tgaaacgatt gccattttat caggcctcct gtatattttt atgggtcatc
     5761 ccatttacat tgttcatttt atcacttttt gccaaaaaaa tctagttttt cccgcagttt
     5821 cgattgattt tcttctaact ccatctatgt aaaccctttc tctccctagt cttggacgac
     5881 ttttggaaaa tctataaaga aggttaaacg attctcctcc atctcgaaac gataagctaa
     5941 tttttcatgt tctaatagac tcttaaccac aaagagcccc ataccagacc ccttgacctt
     6001 gcgactggca ttgtcagaaa aagactgggc tagtttttct tgttcctctg agctacagct
     6061 attttcgata aaaagttctc cttctctttc tccaattcga actaagccac ctggaacaga
     6121 gtgcttaatg gcattgctga tgagattaga aagaatcaac ttcataactg atgggtttag
     6181 ataagcctgc tgatgggtca aactattgtc tatctggagc tctctttcct tggctagcaa
     6241 ggcataatct ttgaccagat ttttcgtcat ctggaggagg tcaattgttt ccctatcatc
     6301 tcgcaattcc tgcacagaag agagggaaag tatctgcaga acatggtgat tgagttcatc
     6361 cacaatcccc aaggcaactc ccagatactg gtctctatcc ttataacgac cgatattctc
     6421 tctcatattt tcgattagga ttttcaaact agccagcggt gttttcaatt catgagaagc
     6481 tcctcgtagg aattcgacct tcatcttctc cagctggaga atggcttcat tcttttcatt
     6541 caagtccgca ataacagtca agagatgctg gtagaggcta ttgatttgtt ccttgagatt
     6601 acctatctca tccttagaat ccacgcgcaa tcgcacttgg gaatccaggt ccatcatccg
     6661 acgggtcacc cgcttgattt ccaaaatcgg tgcaacaata gtccaagcgt agatgtaggc
     6721 caccaagagg gaaatcagaa aggaggccag caaggtatag ggaagaaact ggagactgat
     6781 ttgctccgct tccttttgta aatccatgga agctagaaac tggagaatca tagtaccacc
     6841 gtcttgcgtt ttcacctcgc gctcctcaat aaagagagag gttgtctggc ggtctgtgtc
     6901 cagaggaaga ctgtccttga cttctaactt gtcctcggtc atctcacctt tgacggttcc
     6961 cttgatatca ctagtctggg aatacaagtc taacacttgc tcgatactct gcctatcttt
     7021 cccttctagg gactgggcaa tggctgttgc cttttgacca atggtttcct gacgatgact
     7081 cagataagtc gaaggaaaaa gaaaataaat agctaaatga aggcagataa ccagaacact
     7141 aaatatcgag aaggtataga taaatatctt tgcaaataaa cctgttcgtt tcattttcgc
     7201 tccaatttat aaccaacatt gcgcacagtg aggatacaat ccaagtctag ctttttccgc
     7261 aatttctgga tataaacatc aataacacgg tcaaagggaa cctcatctgt cgctttccag
     7321 acggcatcga taatctgaga tcgagtcaag gcccggcctt catttttcac tagatagtcc
     7381 agaatttcca actctttggc attgataggc acttcttgac ctgcgaggct tgcactgtag
     7441 ctttcaaagt ccaccttggt atccttgtaa gaaaagattc gtcctgtatc gtagtagcgc
     7501 ttgaaaatcg cgtccaccct cacttttaag agggagaggg agaaaggttt ttccagatag
     7561 ccatctgcca aagaagcaaa ggcactcatc ttgtattcct catcctgaaa agctgtcaac
     7621 atcaagacag gaacctgact ggttttacga atctcagcta ggacttctaa gccgttaagc
     7681 ttgggcatct ggatatccag taaaaccaag gccacctcat agctagaaaa ttgctccaga
     7741 gcttcctgac cgtccgctgc ctcaatagtt tcatagccac aatccgtcaa ataatcactg
     7801 accccctcac ggatcatctc ttcatcttct acaattaaaa ttttcatact ttaactgctc
     7861 tctatttttt atttttctta gaataaatac ctactctatt ttctattata gtctcttgct
     7921 ggccttttgt ctgtaagcaa ctgaccacta gataaaacgt tgtgaaattc ctttctcata
     7981 aattccataa ctttagtata ttatatttaa gcactaaagt acaaagaaag caactgaaag
     8041 caatgatttt caccactgct ttcggattta ttttgaattg ttaaatagcc attcctatcc
     8101 actattcttg aatagaaaca caagatgcaa tctttattcc agactcattt tttcaaattt
     8161 attcaccatc cagcaagagc tcttttggtt gttttctaag gagattgctt gaagcaagcg
     8221 ccataacgag aaccactaga accaaggcaa ggacaaaaat gatgataaag tctgatgtct
     8281 gaatggaaat gtctaggctc gacaaggtct tgctaaagcc atctacttct gcaccgccac
     8341 caaggttaga ggcttgagcc gccttactag cctgtttggc aacacctgaa gtcacattgg
     8401 caaggacagt gtttccaatt gcacgggcag tgtaattagc taggaagtaa gcagaaacta
     8461 gagcagggat agcaatcaag atagattcgg tgatgaattg acccaagata cttgcctgct
     8521 tgaggccgat agagaggaga attcccactt ccttgcgacg ggcgttgatc caaaggctga
     8581 gcaagagggc aaggaggaga actgagaagc tcaagctacc ccagaagagg aggttggcca
     8641 tcttgtacat accagagata gattgctcaa gagctgggta gttagaggag ctcttgacga
     8701 gtgtgtagct cttccagttg ataccactga tgccattcaa ctctttcata acatcatcca
     8761 agttcttgtc tgctgttaca aagaaggttg cgtccccata aatggctgtg tcttctgtgt
     8821 atccataaag ttttgcagca gtgtgaatgt ctgtaatagc tgtgttttcg taaagttctt
     8881 gtgagtaggt tactgctgac ttattatgac catcaaagag tcccttgatt gtcacttcaa
     8941 ctgtttcctt ggctcctttt tcattatctg catcgtagat attagagtcc agtttaacct
     9001 tgtcccctac tttccagccg tgtttggctg ccaagtcctt gtgcaagagg attttatcct
     9061 tgtcgtcgtt ggttaagtgc tctccttcga ctagtttata agaaccagag acaaacttgt
     9121 cttctttaga ggagtcattg acacctgtaa tcatcaagct acttccaaaa cgtttggcac
     9181 gatcagcagt gagattcttc ttggtttctg gcgtttcaat caggtcatat ccagtcaaat
     9241 ctccgatagc gttgatacgt ttgacataag actcaatggc cttgttttcg gtgatttttt
     9301 tgatgtcttc acccttgata ttcccagcac cacgaggcgt tccttggttg acgcgacgat
     9361 tgatttgcat ggagaagcta ttggtgatat ttttaaaggt ctcctgagaa gccttggcag
     9421 tagctccctt gattgacaag ccgaccaaac tcaagctcgc catgaggaga ataatcagga
     9481 agatgacaat cgatttgaaa aacttccttg taacataagc aaatgcgttg tgtaacatag
     9541 attccctttc tagattttgt tttaatcatt ctatcaaaat aagctcaaat tatttactag
     9601 tattgcgcgt ttcagtcagt ttcttatcct ttaattcaag tgtaatatct gacgcttgtg
     9661 ccacttcttt actgtgagtt acgacaatca cacatttacc tgttttctgg gcaagtgatt
     9721 tgagtagttc gacaatatct ccagcagttt taggatccag atttcctgtt ggctcatcag
     9781 ctagaataac tggagcttct gagaccaaac tgcgagcaat ggcaacacgt tgctgttgac
     9841 cacctgataa ctggagaaca ttccgcttga tctggctttc atccaaacca agctcaagaa
     9901 gtgtattctt gcttgccttt ttgttgacca atcggatatt ttccagcgga gaaagataat
     9961 ctatcaagtt ataattttga aagaccaggg aaatatggtg catgcgatgg taagaatagc
    10021 ccttcttacg aatatcctct ccttgaaaaa ggatagaacc ttcaacagga ctatctagac
    10081 cagcaagtag ggacaagagt gtggattttc ctgctcctga ctccccaata atactgtaaa
    10141 attttccggg ttcaaaatta taattgatct gatataggac tgcttcagca gtattcttat
    10201 aacggtaggt aacatcttgt aattgtaata aagtcatgat ttctccttct taactaatag
    10261 atgataaaat ttctttcggt gattttctaa ataagaatag gaaacaaagg gctacagata
    10321 agcaactaag cagaactaga aaaacatagg attctgcaaa agataagatg ctagttgata
    10381 aactgcttgc tttggctagt gtatcttgta agcttgcctg atctccactt gctagtagag
    10441 tttggagtag gtaagttgtg attgcgtttc ctgcaacaaa tgctggaagc aaagctccaa
    10501 gagataccaa aactacctct aaacagaatt gtaggaagat cgagctcttg ccttttccaa
    10561 gtgcaagtaa aatccccact tcatagaccc gttctctcaa ccagagagac aaaaccagaa
    10621 ttaaggctcc agctcctgct atcaacatcc cataaaggaa gatggtcagg aaggtttgga
    10681 aagttgcaac tgagtctttg atttgttcaa aagccttgtt ttccttttcg acttggtagc
    10741 cttgattttc caaggccaag ttttctacct gcttcatgag tccgtccatt tccttaggat
    10801 tttctacata gaagcgtgct gcactgactt gagcttcact attgcccaaa agggtttggc
    10861 tactttcata gtctgtaaag acttgatttt cactgaagtc agaagacaag cctgtgaatt
    10921 tctcttgttt tttaccagaa aagatgccga taatctcaaa ctctactgtt tgtccttttc
    10981 cagattcaga ctgaccagca tccaagccaa tcttgtcatg aagcgaaaga ccgttcttct
    11041 tagccaattc ttcgtggata aggattttct tggaatcccc tttttgaagg tgtcgccctt
    11101 cttttagatt gaaagccgaa ctggtaaagg ttacatcctt ggatgaatcc tcaagagccg
    11161 ttaagctaac caagttattg tctgcagctg ataaatcatc acgctccacg ctctgctcgc
    11221 cagtcactgc ttccttgtct tttagttttg cgaccgtctc aagttcagga gagacatttt
    11281 ccagcccctt aatcttgctt acagatgcta ggtctgacaa cttgaatgtc tgaccattct
    11341 ctatcttctt aatagaaaaa gatgtattga gtgatttata aagattgctt tctactgttt
    11401 tgttggactt catcagagtc aaacaggctg aaattccggc caataagacc aataaaatca
    11461 gaaataaaat aaaacttctc agtcgctttc tgctgacata agcccaagat ctttggattg
    11521 gattcatttg tcacctccat atttgtaaga ctattataaa acccaaatat gaaatgttta
    11581 tgaaatacga aaaaaaatat cgagtagggg ataatctcta gcccctctca caccaccata
    11641 cgtgccgttc ggcatacggc ggttcaacta acttttaacg catgtcgttc aaggtaataa
    11701 tccaaacacg aaaccagtcc acgtttttca aggactggtt ttgatatggc acgtttaagt
    11761 accgacttct gagctactat agtagattga aactagaata gtacatctct tcttctaaaa
    11821 tattgttaga aatcgat
//
LOCUS       DAVKWS010000057        11518 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKWS010000057 DAVKWS010000000
VERSION     DAVKWS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.058"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            111..764
                     /locus_tag="VW852_001732"
     CDS             111..764
                     /locus_tag="VW852_001732"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV0272823.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR"
     gene            774..1238
                     /locus_tag="VW852_001733"
     CDS             774..1238
                     /locus_tag="VW852_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0272824.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDIHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1251..2519
                     /locus_tag="VW852_001734"
     CDS             1251..2519
                     /locus_tag="VW852_001734"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV0272825.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     NATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2533..2793
                     /locus_tag="VW852_001735"
     CDS             2533..2793
                     /locus_tag="VW852_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272826.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2804..4165
                     /gene="pdrM"
                     /locus_tag="VW852_001736"
     CDS             2804..4165
                     /gene="pdrM"
                     /locus_tag="VW852_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV0272827.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4319..5287
                     /locus_tag="VW852_001737"
     CDS             4319..5287
                     /locus_tag="VW852_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV0272828.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5303..6295
                     /locus_tag="VW852_001738"
     CDS             5303..6295
                     /locus_tag="VW852_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV0272829.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6513..7556
                     /locus_tag="VW852_001739"
     CDS             6513..7556
                     /locus_tag="VW852_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV0272830.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7620..9305
                     /gene="lpdA"
                     /locus_tag="VW852_001740"
     CDS             7620..9305
                     /gene="lpdA"
                     /locus_tag="VW852_001740"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV0272831.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9369..10358
                     /locus_tag="VW852_001741"
     CDS             9369..10358
                     /locus_tag="VW852_001741"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV0272832.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10425..11495)
                     /gene="xerS"
                     /locus_tag="VW852_001742"
     CDS             complement(10425..11495)
                     /gene="xerS"
                     /locus_tag="VW852_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV0272833.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3390 a         2172 c         2610 g         3346 t
ORIGIN      
        1 tattcaaatc gtatatagtc gatttattaa atctttagat aaactgtaaa aagtggtttc
       61 catagccact ttttgctata atagaggcag taaaaatgaa ggagtcggct atggaacact
      121 cgtcttggca tgctttgatt aaggcgcaat tacctgaggg ttatttcggg aaaatcaatc
      181 agtttatgga gcaggtctat tctcagggga ttatttatcc acccaaggaa aaggtttttc
      241 aggctctctt gacaacactg cttgaagaag ttaaggtggt aattctaggg caagacccct
      301 atcacggacc aggtcaagcg cagggcttga gtttttccgt acctgactct atcccagctc
      361 caccatcctt gcaaaatatc ttgaaagaat tgtcagatga tatcggagtt aagaaatctc
      421 atgatttgac agcttgggct gagcaaggag tcttgcttct taatgcttgt ttgactgttc
      481 ctgctggaca ggccaatggt catgctggtc aaatctggga gccttttact gatgctgtga
      541 ttcaggtggt caatcatcta gatagaccag tcgtttttgt actctgggga gcttatgcac
      601 gtaagaagaa ggccttagtt accaatcctc atcacttgat tattgaatca gcccatccaa
      661 gtcctttgtc ggtttataga ggattttggg gttccaagcc tttttccaag gccaatacat
      721 tcttaaaaga gacaggacaa gagccaatcg attggcttag ataaggagaa aatatgcctc
      781 agttagcgac gatttgttac attgataatg ggaaagaact gctcatgctc catcgtaata
      841 agaaacccaa tgatatccat gaagggaaat ggattggtgt gggtggtaag ctagagagag
      901 gagagacgcc ccaggaatgc gcggtacgtg aaatccttga agaaacaggg ctcaaagcca
      961 agccagttct aaaaggtgtc atcacttttc ctgaatttac accagattta gactggtaca
     1021 cctatgtttt taaggtgacg gagtttgagg gcgacttgat tgactgcaat gaggggacgc
     1081 tagaatgggt tccctatgac gaggttttga gcaagccgac ttgggaaggt gaccacacct
     1141 ttgttgagtg gcttttagag gataaaccct tcttttcagc caagtttgtt tatgatgggg
     1201 ataaattgtt ggatacccaa gttgatttct atgaataaag gagaaagcag atgctactaa
     1261 tcaaaaatgg tcgtgtaatg gatcctaagt ctggtttgga tcaagtttgt gatgtcttag
     1321 ttcaagatgg gaaaattatc aaaattgcgc ctgagatcac ggaagaagga gcagaaacga
     1381 ttaatgctac tggtcttgtg gttgctcctg gtttggtcga tattcatgtg catttccgtg
     1441 aacctggtca aacacataaa gaagacattc atactggtgc cctagcagcc gctgcaggtg
     1501 gttttactac tgtcgtcatg atggctaata ccagtccaac catttcagac gtggagactt
     1561 tgcaagcagt tctccagtca gctgccaaag agaagattaa tgtcaagaca gttgcgacca
     1621 ttactaaaaa ctttaatggt aaaaacttga ctgactttaa ggcactctta gaagctggtg
     1681 cggttggttt ctctgatgac ggtattccgc ttgagagcag taagattgtc aaggaagcca
     1741 tggaggaagc caaaaaactc aataccttta tcagccttca tgaggaagat ccaggtttga
     1801 acggtgttct tggctttaat gaaaatattg ctagagaaca tttccatatc tgcggtgcta
     1861 ctggggtggc tgagtacgct atgatggcgc gtgatgtcat gattgcctat gcaactaaag
     1921 cccatgttca catccagcat ttgtctaagg aagaaagtgt taaagtagtg gagtttgctc
     1981 aggggttagg tgcagaagtc acagcagaag tagcgccaca gcatttctct aagacagaag
     2041 cacttctttt aacacaaggt agcaatgcta agatgaatcc accgcttcgt ttggaatcag
     2101 accgtcgtgc cgttatcgaa ggtctcaaat caggtgtcat cacagttatt gcgactgacc
     2161 acgcgcctca tcatgtagat gaaaaaaatg ttgaggatat taccaaagcg ccatctggta
     2221 tgactggctt agaaacatcc ctgtctctca gcttgaccta tttagtagaa gctggtgagt
     2281 tgagcttgat ggaattactt gaaaaaatga catacaaccc agccaagctt tacaactttg
     2341 aagcaggtta cttggctgag aatggtccag cagatatcac tatttttgat gccaaggctg
     2401 accgctttgt ggactcccat tttgcttcca aagcagctaa ttcaccattc atcggtgaaa
     2461 ccttaaaagg gcaggttaaa tataccatct gtaagggaca aatcgtctat caagcttgat
     2521 aggagttaga atatgaatta ttttcgaaaa cgtagggaga gacaagcgaa atctaatagt
     2581 ggaatttact gtcccgcagc taatcgttcc aaaattcaat atgaaactaa agaaaaagct
     2641 gaccatgcgg ttcaatatga taacggaggt ttagttagaa gctattattg tcgtacatgt
     2701 gcgtgttggc atactacttc aaaatctaat aaacctccgc taagtttgaa aaactatggt
     2761 ctgaaactct ttggtttaga taaatcagaa tagaaagaga agtatgtata agacaaagtg
     2821 tttacgagag aagttagtat tatttttaaa aattttcttc ccaatcctga tctaccaatt
     2881 tgccaattat tctgcctctt ttgttgatac tgcaatgaca ggtcaataca acactatgga
     2941 cttggctggt gtatctatgg caaccagtat ctggaatcct ttctttacat ttctaacagg
     3001 gattgtgtca gccttggtgc ctatcattgg tcaccatctt ggtcgaggca aaaaggaaga
     3061 agttgcgtct gatttttacc aatttattta tttggccttg ggcctatctg tggtcttgct
     3121 ggggatggta cttttcttgg caccaataat cttgaatcat attgggttag aagcagcagt
     3181 agcggcagta gcggttcgct atctttggtt tttatctatc gggattatcc ccttgttgct
     3241 ctttagcgtc attcgttcct tgctggattc gctgggcttg accaaactgt ccatgtacct
     3301 catgcttttg ttactccctc tcaatagcgg atttaactat ctcttgattt acggtgcctt
     3361 tggtgttcca gaactgggag gggctggtgc tggtttagga acatccttgg cctactgggt
     3421 cttgcttggg atttctgttc tggttttatt taaacaggag aagctcaaag ccttacacct
     3481 tgagaaacga attccactta atatggataa aattaaggaa ggagttcgtt taggtctgcc
     3541 tattggggga actgtcttcg cggaagtggc tatcttttca gtggttggct tgattatggc
     3601 taagttttcg cccttgatta tagctagtca ccagtcagct atgaactttt caagtcttat
     3661 gtacgccttt cctatgagta tctcatcggc tatggctatt gtcgtttcct atgaagtggg
     3721 agccaagcga tttgatgatg cgaaaaccta tattggtcta ggaagatgga ctgccctcat
     3781 ttttgcggcc ttcaccttaa ccttccttta catttttagg ggaaatgtgg ccagtcttta
     3841 tggtaacgac ccaaaattta tcgatttgac agtgcgtttt ttaacttata gtcttttctt
     3901 ccagttagca gatacctttg cggcgccgct tcagggaatt ttgcgggggt ataaggatac
     3961 agttattcct ttttaccttg gtttgcttgg ttattggggc gtagcaatcc ctgtggctac
     4021 gctatttgat tccctaacag attttggagc ttattcttac tggatcggct tgattattag
     4081 tttgattgtg agcggggcgc tctaccgttg gcgtttaact gtgattatga agagatttga
     4141 atctttagca aaatccaaat gctaaaaagt ttgtgaaaaa atattcttga caagaaaatc
     4201 ccttgttttc acggggtttt cttttttatt ttgtgaaatt tcttttagaa aaactttgac
     4261 agtatttgcc aaaagcggta aaatagtaag gtaagaagaa agttagaaag gcaagaagat
     4321 gtcaacttta gataaaaatc ttttgctaga aatgttccgt aagatggaag aaatccgtcg
     4381 catggactta aaaattgcac aattagtaaa gaaagggaaa gtgccaggaa tgacgcactt
     4441 ttctgttggt gaagaggcag ctaacgtggg ggctatgtta gctctcaatc cagatgatct
     4501 gattacctca aaccaccgtg gacacgggca agctattgct aaagggattg acctcaacgg
     4561 aatgatggct gaaatccttg gtaaatacac tggaacctgt aaagggaaag gtggatctat
     4621 gcatatcgct gaccttgatg ctgggaacct tggtgccaat ggtatcgtag gtggtggtat
     4681 ggggatcgct gtcggtgcag ccctcagtca gcaaatgcaa aataccggta aaatcgttgt
     4741 ctgcttcttt ggagatggtg cgaccaatga aggtgttttc cacgaagcag tgaacatggc
     4801 ttctatctgg aacctgccag tcattttcta ttgcattaac aacggttacg gtatctctgc
     4861 ggatatcaag aaaatgacca atatagaaca tatccatcaa cgtagcgccg cttatggaat
     4921 tcctggaatg ttcatcgaag acggtaacaa tgtcatcgat gtctatgaag gatttcagaa
     4981 agctgtggac catgttcgca gtggcaatgg tccagtcttg attgaaagtg taacttatcg
     5041 ctggctcggt cactcatcat ctgaccctgg taaatatcgt acgcgtgaag aagtggaatt
     5101 gtggaaacaa aaagatccaa tcgaaaacct ccgcaattac cttattgaaa ataacattgc
     5161 aagtgccgaa gaattggaag aaatccaagc gcaagtaaag gaagcagtag aagcttctgt
     5221 taaatttgca gaggaaagcc cattcccacc gcttgaatca gcctttgaag atatttacgc
     5281 agactaagga gaaagagata aaatggaaac aaaaacaatg tccttccgtg acaccattat
     5341 ccttgctatg tctgaggaaa tgcgtcgcga tgaaaatgtg ttcttgatgg gagaagacgt
     5401 cggtgtcttc ggaggagact tcggtacttc tgttggaatg cttgaagaat ttggtccaga
     5461 acgtgtccgt gactgtccga tttctgaagc tgccatctct ggagcagcag caggagcagc
     5521 catgacagga cttcgtccaa tcgtcgatat gaccttcatg gacttctctg ttattgccat
     5581 ggacaatatc gtcaaccaag ctgctaaaac acgttacatg tttggtggta aaggtcaggt
     5641 tccaatgact gttcgttgtg cagctggtaa cggagttggc tctgccgccc agcactcaca
     5701 atctctagag tcttggttta ctcacattcc aggacttaag gttgtggcac ctggaacacc
     5761 tgcggacatg aaaggcctgc ttaagtcttc tatccgtgat aacaacccag ttattatact
     5821 tgagtacaag tcagaattta accaaaaagg ggaagtgcca gttgatccag actacacaat
     5881 tccacttggg gttggtgaaa tcaaacgcca aggaacggat gtaacagttg ttacttatgg
     5941 aaaaatgctt cgccgtgtgg ttcaagctgc tgaagaatta gcagaagaag gaatttcagt
     6001 tgaaattgtt gacccacgta cccttgttcc gcttgataag gatatcatca taaactcagt
     6061 gaagaagact ggtaaggttg ttctggtcaa cgatgcccac aaaacaagtg gctatatcgg
     6121 tgaaatttca gctattattt cagaatcaga agcatttgac tatctagatg caccaattcg
     6181 ccgttgtgca ggagaagatg tgccgatgcc ttatgcacaa aacctagaaa atgcaatgat
     6241 tccaacagtt gaaagtatca aagatgcaat ccgaaaaact tataacaaag aatagggtta
     6301 cataagataa gttatgtaaa taaaataaag ctgacgagaa actttgtatc ttttaggtgc
     6361 agagctcttc gtcattaata tcttagatta gagcacgggc taaaagatcg gaaaaaagat
     6421 aaatctcctt ggcttcatcg aagccatcgt cgatttccta tttttcagtc gcttttgacg
     6481 ccctttgtat cataaataga attggagagg tcatggctga tgacaagcta agagcgactc
     6541 ctgcagctag aaagttagcg gatgatttag ggataaacct ctacgacgtt tctggctcag
     6601 gtgcaaacgg tcgtgtccac aaagaagacg tggaaactta taaagacaca aacgtggttc
     6661 gcatttcgcc acttgcaaaa cgaattgccc tcgaacataa cattgcttgg caggaaatcc
     6721 aaggaaccgg tcatcgtggt aaaatcatga agaaggatgt tttggccctg cttcctgaaa
     6781 atatcgaaaa cgatagcatc aaatctcctg ctcagattga aaaagtggaa gaagtccctg
     6841 ataacgtaac accatatggt aaaatcgagc gtattccaat gacaccaatg cgtaaggtta
     6901 ttgcccaacg catggttgaa tcttacttga ctgcgccaac cttcaccctc aactatgaag
     6961 ttgatatgac tgaaatgttg gctcttcgta agaaggttct tgagccaatc atggaagcaa
     7021 ctgggaagaa gactactgta acagaccttc tttcgcttgc agttgttaag actcttatga
     7081 aacacccata catcaacgct tcattgacag aagatggcaa gactattatc actcacaact
     7141 atgtcaatct tgccatggca gttgggatgg ataatggatt gatgacacct gttgtttaca
     7201 atgctgagaa gatgagtctt tcagaactgg ttgtagcctt taaggatgtt attggccgta
     7261 ccttggatgg taaattggct ccaagtgagt tacaaaattc aacattcaca atcagtaatt
     7321 tgggaatgtt tggtgttcag tcctttggtc cgattattaa ccaacccaac tcagctatcc
     7381 ttggtgtcag ttcgacaatc gagaagccag ttgtcgtcaa tggtgaaatt gtgattcgcc
     7441 caatcatgag tttaggatta accattgacc accgtgtcgt agatggtatg gctggtgcta
     7501 agtttatgaa ggacttgaaa gagttgattg aaacgccaat ctcaatgtta atttaagagc
     7561 aagagtattc atttttaaga tatagataaa ggaaggaaga catggcctta gaagtaatta
     7621 tgccaaaagc cggcgtggat atgacagaag gacaaatcgt ccaatggaat aaaaaagtcg
     7681 gagaatttgt aaaagaagga gaaatccttt tggaaatcat gactgataaa gtcagcatgg
     7741 aattggaagc cgaagaagat gggtacttga ttgccattct caaaggagat ggtgaaactg
     7801 tccctgtaac ggaagttatc ggttaccttg gtgaagaaag ggaaaacatc ccaacagctg
     7861 gagcagcatc gccagaagct agccctgtac ctgtagcaag tacctcaaac gatgatggta
     7921 agagcgatga tgcttttgat atcgttgtga ttggtggagg tcctgctggt tatgttgcag
     7981 ccattaaagc tgcccaactc ggcggtaagg ttgcccttgt tgagaaatct gaacttggtg
     8041 gaacctgctt aaaccgtgga tgtattccaa ccaagaccta ccttcataac gctgaaatta
     8101 ttgaaaatat cggtcatgct gcaaatcgtg gtatcgtgat tgaaaatcct aacttcactg
     8161 ttgatatgga aaaactttta gaaactaaat ctaaagttgt taatactctt gttggtggtg
     8221 ttgcaggact tcttcgtagc tacggagtta ctgttcataa aggaattggt acaatcacta
     8281 aagacaagaa cgtcttggta aatggctctg aattgcttga aaccaagaaa atcattcttg
     8341 ctggtggttc aaaagtcagc aagatcaatg tccctggtat ggaatctcca cttgtcatga
     8401 ctagtgatga cattcttgaa atgaacgaag tgccagaaag ccttgttatc atcggtggtg
     8461 gagttgtcgg tatcgaactc ggtcaggcct tcatgacatt tggttcaaaa gtgactgtta
     8521 tcgaaatgat ggaccgtata gttccagcta tggatgcgga agtttctaag aaccttcgct
     8581 tgatccttga gcgtaaagga atgaccatct tgactggtac taaactgcaa gaaatcattg
     8641 aggaaaatgg tcaacttcgt atcaaggttg aaggaaaaga cgatatcatc gcaagcaaag
     8701 ctcttctttc aattggtcgt atgccagacc ttgaaggtat tggagaggtt gagtttgaat
     8761 tggatcgtgg ttgtatcaag gtcaatgaat acatggaaac ttcagttcca ggcatttatg
     8821 caccaggtga catcaacggt actaagatgt tggctcacgc agctttccgc atgggtgaag
     8881 tttccgctga aaatgccctt aaaggaaatc atgcagttgc caaattgaat ttgactcctg
     8941 cagccatcta cactctccct gaagtagcag cagtaggttt gacagaagaa caagcccgtg
     9001 agaaatacga tgttgccatc ggtaagttta actttgctgc taacggtcgt gctattgcat
     9061 ctgacgcagc tcaaggtttc gtaaaagtta tcgctgataa gaaatacgga gaaatccttg
     9121 gtgtacacat tattggtcct gcagccgcag aattaataaa cgaagcatca agcatcatcg
     9181 aaatggaaat cactgttgag gaaatgctga agaccatcca cgggcaccca acttactctg
     9241 aagtgatgta cgaagcgttt gcagatgttc taggaatggc catccattca cctaagaaaa
     9301 aataaaacga agatagatta gactggaagg ttatttccag tctctatcat ataaagtaaa
     9361 gggatatcat gaaatacatt atcaatcatt caaatgacac tgcttttaat attgccttgg
     9421 aagaatacgc ctttaaacac cttttggatg aggatcaaat cttcctactt tggattaaca
     9481 agccatctat cattgttggt cgtcaccaga acactatcga agaaatcaac cgtgattatg
     9541 ttcgcgaaaa tggtattgag gtagttcgcc gtattagtgg tggtggagct gtttaccacg
     9601 atttaaataa cctcaactac acgatcatct ccaaagaaga tgaaaataag gcatttgact
     9661 tcaagagctt ctcaactccg gttatcaata ccctggctca acttggggtt aaagctgagt
     9721 tcacaggtcg taatgacctt gagattgatg gtaagaagtt ctgtggcaat gcccaagcct
     9781 atatcaacgg ccgtatcatg caccacggtt gcttgctctt tgacgttgat ttgtcagtcc
     9841 tcgcaaatgc ccttaaggtt tcaaaagata aatttgaatc aaaaggtgtg aaatccgtcc
     9901 gtgcccgtgt aaccaatatt atcaatgaat taccaaaaaa aatcacagtc gaaaaattcc
     9961 gtgatttact cctggaatac atgaaaaaag agtacccaga gatgactgaa tacgtctttt
    10021 cagaagaaga attggccgaa atcaatcaca tcaaggatac taagtttgga acttgggact
    10081 ggaactatgg taaatcacct gaatttaacg tccgtcgtgg aaccaaattc actagtggta
    10141 aggttgaagt ctttgctaac gttactgaat caaaaatcca agacatcaag atttatggtg
    10201 acttctttgg tattgaagac gttgctgcag tagaagatgt ccttcgtggg gtgaaatacg
    10261 aacgcgaaga tgttcttaag gcgctaaaaa ccattgatat cacacgctac ttcgctggta
    10321 ttagccgtga agaaatcgct gaagcggtag ttggataaat caaaaaaaga agttagccaa
    10381 cttctttttt tgatatttat ttacataatt taaaatacgt aaaatcataa actatctaga
    10441 gcattctttt gttcatcatt aacaatatgg gtatagaggt cagtgacttg tgtgctggca
    10501 tgtcctagtt ggtgactgac taaaacttgt gatttagtcg catcatagag cctagttgct
    10561 agcgtatggc gcagtttatg gggtgttaca cgcactttaa aatcctctga gtatttagca
    10621 accattttct caacgctaga agcatcgata cgattaggaa cacctctgta gagagttaaa
    10681 aaaagggctg tatctgtttt ttccgtttta tagcgttgat tccgaatggc cagataattc
    10741 tctaaataag gtttagcaaa agcagcgaca ttgactgagt cacgtttgcc accttttcga
    10801 gtaacatcaa taaccatcat ttttagattg agatctctta gatctagatt aacagcttca
    10861 gataagcgaa caccagatgc caagagaagg gcaataatgg ctaaatctcg ttctttattt
    10921 ttgttgaatg atgagagagc tcgatttgaa agctgttgtg ggtgctcttg atcgatataa
    10981 gttagaaaac cttctgtttc atcacctaga aagagttttt gcttgatatt ttcagctctg
    11041 gcagcaaggg tttctttctt tttcttggtt gaaacttttt tcattacatt acgatagaaa
    11101 taaggttccc cctgatcgtt ttcaacctcc tcggttagat acttgtaaag actagaaagt
    11161 gctgataagg ttcgattgat agttgtctgt gaaacacctt gttttgttgt attagcattc
    11221 agcaagggac gttcacgtag ataaaggata aaggattcca tgtctttctt agacatattt
    11281 tccaaaactg ataaaggaat atcggatatt ttatcagcgt ttgaaatacc agactccaaa
    11341 acccagctga aaaatcggtc atattcctta aggtattcgt acagggttgt aaaactgtag
    11401 ggcacagcca gcttagattg gtagtattcc agaacatacc agggcatgag ttgttttagt
    11461 ttgtcgattc gttccagtaa aatctcacgt ttcatgtatt ttctccataa gtaagtat
//
LOCUS       DAVKWS010000058        11118 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKWS010000058 DAVKWS010000000
VERSION     DAVKWS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.059"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            89..451
                     /locus_tag="VW852_001743"
     CDS             89..451
                     /locus_tag="VW852_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272834.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVVLLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            986..1678
                     /locus_tag="VW852_001744"
     CDS             986..1678
                     /locus_tag="VW852_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV0272835.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1731..4142
                     /locus_tag="VW852_001745"
     CDS             1731..4142
                     /locus_tag="VW852_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV0272836.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYGFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4392..5093
                     /locus_tag="VW852_001746"
     CDS             4392..5093
                     /locus_tag="VW852_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272837.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5095..7803
                     /locus_tag="VW852_001747"
     CDS             5095..7803
                     /locus_tag="VW852_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0272838.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7899..8390)
                     /gene="tpx"
                     /locus_tag="VW852_001748"
     CDS             complement(7899..8390)
                     /gene="tpx"
                     /locus_tag="VW852_001748"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV0272839.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8513..9442)
                     /gene="psaA"
                     /locus_tag="VW852_001749"
     CDS             complement(8513..9442)
                     /gene="psaA"
                     /locus_tag="VW852_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV0272840.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9474..10322)
                     /locus_tag="VW852_001750"
     CDS             complement(9474..10322)
                     /locus_tag="VW852_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0272841.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10319..11041)
                     /locus_tag="VW852_001751"
     CDS             complement(10319..11041)
                     /locus_tag="VW852_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0272842.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3261 a         2069 c         2534 g         3254 t
ORIGIN      
        1 tttattttat aatatcagaa aatacttttt ttaaaaagca aatatgatac aattttattt
       61 gaaaaaaaat aaaaaaggag attttattat gaaatcaagt ggtatcgtcg gtcttgtggg
      121 aggaatttta cttcttattt gtccttttgt cttgttggga acagcggtaa atacagctgc
      181 tacaactctt aatggaggag ctactgcagg ggctttttca ggtgttgtct tactcttgaa
      241 tgccttgaag attgcaaatc ttgttcttgg tatcattgct attgtttact ataaaggaga
      301 taagcgtgta ggtgcagctc cgtctgtact aatgattgtt tctggtgaag ttagtctcat
      361 tccattctta ggatgggttg gggggattct tgctattatc ggaggatctc tattccttgc
      421 aacattgaag aaattcaaat cagaagaata aaaggtattt tagcatgaaa agaacaaaaa
      481 agtttatcgg tataggagta gctctattat ctctttctct tctagttgca tgtggaacat
      541 aaagttcaaa gaatacttca acaagtaatg atgagaagac agtagcaaca tccaatagtt
      601 caaaagaaac aatcactttc gatacaccgg ttgtaacaga cgatgcgatt gaatcaatac
      661 gcacttatgc agattatata gatctttata aaaatatttt tgatgattat tttactaaag
      721 ctgaggaagg tttcaaaggc acagctatgg aaaataatga ctcgtttact aaactaaaag
      781 agtcaactca aaaattattc gatgcgcaga aaaaaaggtt aaataatgaa gatagaatag
      841 aaacaaccaa aaacaatgtg attgccaaac attgtcaaac agtcttttcc tttttggttt
      901 tgactagctt ttttgtgaaa aattgtgtaa aatagaatag ataaacgagg ggaaacctcg
      961 gaaaatttaa aggagaatcc atctaatggt aaaattggtt tttgctcgcc acggtgagtc
     1021 tgaatggaac aaagctaacc ttttcactgg ttgggctgat gttgatttgt ctgaaaaagg
     1081 tacacaacaa gcgattgatg ctggtaaatt gatcaaagaa gctggtatcg aatttgacca
     1141 agcttacact tcagtattga aacgtgctat caaaacaact aacttggctc ttgaagcttc
     1201 tgaccaattg tgggttccag ttgaaaaatc atggcgcttg aacgaacgtc actacggtgg
     1261 tttgactggt aaaaacaaag ctgaagctgc tgaacaattt ggtgatgagc aagttcacat
     1321 ctggcgtcgt tcatacgatg tattgcctcc aaacatggac cgtgatgatg agcactcagc
     1381 tcacacagac cgtcgttacg cttcacttga cgactcagtt atcccagatg ctgaaaactt
     1441 gaaagtgact ttggaacgtg ctcttccatt ctgggaagat aaaatcgctc cagctcttaa
     1501 agatggtaaa aacgtattcg taggagctca cggtaactca atccgtgccc ttgtaaaaca
     1561 catcaaaggt ttgtcagatg atgagatcat ggacgtggaa atccctaact tcccaccatt
     1621 ggtattcgaa ttcgacgaaa aattgaacgt tgtttctgaa tactaccttg gaaaataaaa
     1681 aattgtaagc ctaggattga tttctaggct ttttatgtta gtatggaagt atgataagga
     1741 ataaaaaaca agattatgta ctggcctaca agcaaccggc ttcaaccact tacatgggtt
     1801 gggaagaaga agctttaccg ataggcaatg gttctttagg agcaaaagta tttggcctta
     1861 taggggctga acggattcaa tttaatgaaa aaagtctctg gtctggaggt ccacttcctg
     1921 atagttcaga ttatcagggt ggaaatcttc aggatcagta tggtttttta gctgagattc
     1981 ggcaggcttt ggagaagaga gattacaatc tggctaagga actggctgag cagcacctaa
     2041 ttgggccaaa aacgagtcaa tatgggacct atctgtcttt tggggatatt cacattgagt
     2101 tcagccagca aggtacgact ttgtctcagg tgacggacta tcagagacag ctgaatatta
     2161 gtaaggcact tgcgacgact tcttatgtct ataagggaac gcgatttgaa cgtaaagctt
     2221 ttgcgagttt tccagatgat ctcttggttc aatgttttac taaggaaggg ttggaaactc
     2281 tagattttac tatagaacta tccttgacct gtgatttggc ttctgatgga aagtatgagc
     2341 aggaaaaatc tgattacaag gagtgtaagt tggatattac tgattctcat atcttgatga
     2401 agggaagagt taaggataat gatctgcggt ttgctagtta tctagcttgg gaaacggatg
     2461 gagatattag agtttggtca gatagggttc agatatcagg agccagttat gccaatctct
     2521 tcttggccgc taagacggat tttgcccaaa atcctgctag caattatcgc aagaaactag
     2581 atttagagca acaggtgata gacttggtgg acacagctaa agaaaagggc tatacccaat
     2641 tgaaatcaag gcatatcgag gactaccaag ccttattcca gcgtgttcaa ttggatttgg
     2701 aagctgatgt tgacgcatcc actacagatg atttgttaaa aaattataag ccacaagaag
     2761 ggcaggcttt ggaggagctg ttcttccagt atggacggta tttattgatt agttcgtcca
     2821 gagactgccc agatgctcta ccagctaacc tacagggagt ctggaatgcg gtcgacaatc
     2881 ctccttggaa ttcggactat cacttaaatg tcaatctgca gatgaattat tggccagcct
     2941 atgttaccaa tctcctagag acggtctttc cagtcatcaa ctatgtagat gatttgcgtg
     3001 tctatggtcg tctagcggct gtaaagtatg caggaatcgt ctctcagaaa ggtgaggaga
     3061 atggttggtt ggttcatact caagcgactc cctttggttg gacggcacct ggttgggatt
     3121 actattgggg ttggtcacca gctgccaatg cgtggatgat gcaaaccgtt tatgaagcct
     3181 attcatttta tagggaccaa gactatctca gggagaaaat ttatcccatg ttgagggaaa
     3241 cggttcgttt ttggaatgcc tttttacata aggatcagca ggcgcagcgt tgggtgtctt
     3301 ctccgtctta ttccccagaa catgggccga tttcgattgg caatacctat gaccaatctc
     3361 tgatttggca gttatttcat gattttattc aggctgctca ggaattggga ctggatgagg
     3421 acttgttgac tgaggttaag gagaagtctg atttactaaa tcctttgcaa atcactcaat
     3481 ctggtcgaat cagggagtgg tatgaggagg aagagcagta ttttcaaaat gagaaagtgg
     3541 aggcccagca tcggcacgct tcccatctag tgggactcta tcctggcaat ctctttagct
     3601 acaagggaca agagtatatt gaagcggcgc gtgctagcct caatgatcgt ggagatggcg
     3661 gcacaggctg gtccgaggct aataagatca atctctgggc gcgtttggga gatggcaatc
     3721 gagcccataa attattggca gagcagttaa agacatccac cttgcaaaat ctttggtgta
     3781 gccatcctcc ttttcagata gatggtaatt ttggtgctac tagtggcatg gcagaaatgt
     3841 tactccagtc tcatgcagct tatctggtac ctctagctgc cctacctgat gcttggtcaa
     3901 caggttctgt ttcaggctta atggcacgtg gacattttga agtgagcatg agctgggaag
     3961 ataaaaaact cttacagttg accattttat caaggagtgg aggagatttg cgagtttctt
     4021 atccagatat tgagaagagt gtgattaaaa tgaatcaaga aaaaataaaa gcgaaatgca
     4081 tggggaaaga ttgtatttcg gtggcaacag cagaaggtga tcttgttcaa ttttattttt
     4141 aagaagatgt tataaggcag taatttgaaa ctgcctttta ataaggattt aagaatataa
     4201 gcagttttca actagttgaa aaaacgttat aatgataata ggaagtaata ctcaatgaaa
     4261 atcaaagagc acaaactagg aagctagccg caggttgctc aaaacagtgt tttgaggttg
     4321 cagatggaag ctgacgtggt ttgaagagag attttcgagg agtataattt gtttgataga
     4381 gggtgggtct gatggcttat attgagatga aacactgtta caagcgttat caggttgggg
     4441 acacggagat tgtggccaat cgtgatgtga attttgagat tgaaaagggg gagctggtta
     4501 ttatccttgg tgcttcaggt gcaggcaagt caacagttct taaccttctt gggggaatgg
     4561 ataccaatga tgaaggggaa atctggattg atggtgttaa tattgcggat tatagttccc
     4621 accagcgcac caattaccgt agaaatgatg tggggtttgt ttttcagttt tataatctag
     4681 tttctaatct gacagctaag gaaaatgtgg aactggcttc tgaaattgtg acagatgcct
     4741 tgaattctga tcaggtcttg acagatgtag gtctggctca tcgtctcaat aactttccag
     4801 cccagctttc tggaggggag caacagcgag tctccattgc acgcgcggta gccaaaaatc
     4861 ctaaaattct tctttgtgat gaacctactg gagccttgga ttatcagacg ggcaagcagg
     4921 ttttgaaaat tctccaagac atgtctcgtc aaaagggagc gacggtgatc atcgtgactc
     4981 ataatggagc tttggcgccc attgctgatc gcgtgattca tatgcacgat gccagtgtca
     5041 aggatgtggt gctcaaccag catcctcagg atattgacag tttggagtac tagcatgatc
     5101 aagcgaaaaa cttattggaa ggacttaatt cagtccttca caggctccaa ggggcgtttt
     5161 ttatccatct tgatcctgat gatgttggga tctctagcct tagtaggcct caaagtaacc
     5221 agtcccaaca tggaggcgac agctaatgct tatttaacaa ctgctcaaac cttggatttg
     5281 gcagtcatgt ctaactatgg cttggatcaa gcagaccaag aagaactaaa acagacggag
     5341 ggcgcagagg tcgagtttgg ctatttgaca gatgtgacta tggataatgg gcaggatgcc
     5401 attcgactgt actccaaacc agagcgaatt tcaacctttc agctaaggaa gggacgactt
     5461 cctcagtcag acaaggaaat cgctttggcc actcatttgc aaggccaata cagcgtggga
     5521 caggagatta gttttaaaga aaaagaagag ggtcattcct ctttaaaaga ccatacttat
     5581 accattactg gttttgtgga ttcggctgaa atcctctccc agcgagatat gggctacgca
     5641 ggaagtggaa gtgggactct gacagcctat ggggtgattt tacctagtca gtttgatcag
     5701 aaagtctaca atatagctcg tttgaaatat caagatttag caggtttaaa tgccttttca
     5761 tcagcttatg aagaaaaatc caagcaacat caggaagagc ttgaacaaat tttatcagat
     5821 aatggcaagg tacgtctgca acttttgaaa aaagaaggac aagagtctct agacaagggg
     5881 caagagaccc ttgacaaggc tcagactaat ttgcaggaag gcaagcgtcg tttagcagct
     5941 gctcaagctc gtatacaggc tcaagaaagt caactagcct tgtttcctca agttcagaga
     6001 gagcaggcta gtgctcaact tacccaagcc aagcaggaat tgggcaagga agaggccaaa
     6061 ctaaagcaag ctgaacaaaa tctagcccaa gaaaaggaaa aattagaaaa acatcagcaa
     6121 gtcttggacg atttggcgga gccaaggtat caggtctata atcgtcagac catgccaggt
     6181 ggtcagggct atcttatgta tagcaatgct tcatccagta ttcgagcagt gggcaatatc
     6241 tttcctgtgg tactttatgc cgtagcagcc atggtgacct ttacgaccat gactcgcttt
     6301 gtagacgaag agcgaactca tgcagggatt tttaaggcct tgggttaccg tagtaaggat
     6361 attatcacca agtttctcct ttatggacta gtagctggga ctgtcggaac ggctctaggt
     6421 agtatacttg gtcattattt gctagccagt gtaatttcaa gtgtcattac aaaaggcatg
     6481 gtggtgggag aaacccagat tcagttctat tggacctata gcttactagc tcttgtcttg
     6541 agcttgttgg cgagtgtgtt accagcctat ctggtggctt ggagggaact tcatgccgaa
     6601 gcagcccagc ttctacttcc taaacctcct gtcaaaggag ctaaaatctt attggagcgt
     6661 atcggtttta tctggcgtcg tctcagtttt actcataagg taacagcccg caacatcttt
     6721 cgttataagc agagaatgtt gatgacaatc tttggtgtgg caggttctgt agctctgctc
     6781 tttgcaggtt tgggaatcca atcttctgta gcaggagttc cgtctaaaca gtttcaacaa
     6841 atccaacagt atcagatgct tgtctctgaa aatcctagtg cgaccaatca ggacaaggta
     6901 gagctagcag aagtattgaa agggcaggag atactagcct accagaaaat ctattctaaa
     6961 acgctagaca aggatttcaa aggcaaggct ggtcttcaaa acattactct tatgatgata
     7021 gagaaggaag atttgactcc ctttatccat cttcaacatc atcagcagga gctgacatta
     7081 aaagatggca tcgttattac agctaaactc gcccagctgg caggtgtcaa ggttgggcag
     7141 actttagaaa ttgaaggtaa ggaactaaag gtcgctgcta ttactgagaa ctacgttggt
     7201 cactttattt atatgagtca ggctagctat gagcaacttt acggacagct accccaagcc
     7261 aacacttatc tggtctcatt aagggatacc agtgcaacta gtatcgaaag tcaggcgggc
     7321 ttgcttatga atcaatctgc ggtgtccagc gttgtccaaa atgcttcagc cattcgactc
     7381 ttcgactcta tcgctagctc actcaatcag accatgacca tcttggtcat cgtatcggtt
     7441 ctattagcta ttgtcatcct ttacaatctg accaatatca acgtagctga gagaatccgt
     7501 gaactctcca ctatcaaggt tctcggtttt cataataatg aagtcaccct ctacatttac
     7561 cgtgagacga ttgtgctgtc ccttgtggga atcgtacttg gtctggtagc tggtttctat
     7621 ttacaccaat ttttgattca aatgatttcg cctgcgacta ttctctttta tccgcaggta
     7681 ggctgggaag tctatgtaat cccagtggca gcagtaagca tcattttgac cttgcttggt
     7741 ttcttcgtca attattatct gagaaaggtt gacatgttag aagccctgaa atctgtagag
     7801 taaggtagtt atttttagct gattgaactt ctatttacta atattcaaaa atcctccgtt
     7861 tcaaagagca gggaactctt tgtgacagag gattttttct atagggcttt agcagctgca
     7921 attgcggctt cgaagtttgg ctcagaattg atattatcca cgtattcaac gtagcgaatc
     7981 gtattgtcag tatcgaggac aaagactgcg cgtgctaata gatgccattc gttgatcaag
     8041 agggcataat cgcgcccgaa agaatggtca aagtagtctg aaagcataat ggcattgtca
     8101 aggccttcag caccgcacca acgtttttga gcaaaaggta ggtccattga aacagtcaat
     8161 acgaccgtgt tgtccagtcc agctaattct tcattaaaac gacgtgtttg agttgagcag
     8221 atgcctgtat cgatagaagg aacgacactc aagacttttt tcttgccatc aaaatcagcc
     8281 agagattttt tagaaagatc tgttgtagta agagaaaaat caagcgcctt gtcgccgact
     8341 tgtagttgtt tacctgtaaa gctcacagga tttccgagaa aagttaccat aggatactcc
     8401 aatctttttt cttccattgt atctgaaaca gtcagaattt tccaatgatt tgaccggaaa
     8461 tgtgggcata gaaaaaacgc cagctcacat gagaatgacg tttttcagag gcttattttg
     8521 ccaatccttc agcaatcttg tcaaggttgt atttcatcat gctgtagtag ctgtcgcctt
     8581 ctttaccttg ttctgcgata gagtcagtaa agatttgtgc gtagattggg atgtttgtgt
     8641 cttgagaaac agttttcatt ggacggtcat ccacacttga ttctacaaag agtgatggaa
     8701 cttttgtttg gcgaagtttt tcaaccaagg tcttgatttg ttcaggagtt ccttcttctt
     8761 cagtattgat ttcccagatg taggcacttg gaacaccata ggctttagag aagtatttga
     8821 atgctccttc gctggttaca atgagtttct tttcagcagg gatcttatta aatttatcct
     8881 tactttcttt atcaagtttg tctaacttat cagtatattc tttgagattt ttttcataga
     8941 attctttatt gttagggtct ttggcgctca attgtttggc gatattttta gcaaaaataa
     9001 taccgttttc aaggttaagc caagcgtgtg ggtcttcttt tcctttttca ttttgacctt
     9061 caaggtagat aacatcaacg ccgtcgctga ctgcgaagta gtctttgttt tcagttttct
     9121 tggcattttc taccaatttt gtaaaccaag cattgccacc tgtttcaagg ttgataccgt
     9181 tatagaaaat caaatcagcc tcagaagttt tcttaacgtc ttcaggaagt ggttcgtatt
     9241 cgtgtgggtc ttgcccaatc ggaacgatac tatgaaggtc aattttgtca ccagcaatat
     9301 ttttagtaat atcagcgatg attgagtttg tagcaacaac ttttagtttt tgaccagaag
     9361 ttgtatcttt ttttccgcta gcacatgcta caagaatgat tgcagaaaga aagagaacga
     9421 gtaatgtacc taattttttc attagatcct ccaatttatt agggctttgc cccttatttt
     9481 aacaaatgtt tatttttcag tttcaaatat cgttgtttgg gagcgataaa gaagctaatg
     9541 agaaagaaac tagcagctgt aagcacgata ctagaacctg ccgcaacatt aaaactatag
     9601 ccaataaaga gtcccaaaac tgaagcagta gctccgaagg ttgaggaaag gaaaatcatg
     9661 cttttcagac tattagcata cagataagca gttgcagctg gggtaatcag catggctaca
     9721 atcaggatag ttccgacact ttgcatggct gtcacagaca cgagagtcag gagtaccatg
     9781 agaaggtagt gatagaaatt gacaggcatt cccatggctt tagccaagag ttcatcaaag
     9841 gaagttatca agagttgctt gaagaaaatc cagattaaca agaggatggc tgcccccaca
     9901 cccatagtaa taaacatatc cgtatcttgg acggccagga tattaccaaa aaggatatgg
     9961 aaaaggtcag ttgaactttt agcgacacca atcaagatga taccgagggc taagaaagaa
    10021 gaaaaggtaa tgccgatggc ggtatcgctt ttgataatcg agtttccttt gatgtaggta
    10081 atgatgatgg cagctagcaa tccaaagaca atggctccga taaagaagtc aaggcccaag
    10141 atgaaggaga gggctacacc tggtaagaca gcatgtgaaa tggcatctcc catgagtgac
    10201 atcccgcgta gaataatgaa acatcccaca gctccagcta caatcccgac gacaatagct
    10261 gttatcaagg cattttgtag gaaatggaat ttttgcaatc catcgataaa ttctgcaatc
    10321 ataggtcacc tccattgaaa aagagttgat taccgtaagc ttcttttaga ttggtttcgg
    10381 taaaagtttc ttttgttgga ccaaaggcaa tcacttctcg attgacaagt aagacttgat
    10441 cgaagtagtg gggaatcttg ctgaggtcgt ggtgaacgat gagaaccgtc ttcccagctt
    10501 ttttcaaatc tctcagcgta ttcatgatga tttcctcact gacagagtca atcccagcaa
    10561 agggttcatc caagaggata tagtcggctt cctgcaccaa acatctggca atcaagaccc
    10621 gctggaattg acctccagac agttgactaa tttgacgttc agcgtagtca gctaggccga
    10681 cgatttcaag ggcctcttgc actttcttcc aatgtttagc ctttaaactt cgaaagagag
    10741 gaatagaggg aaatagtcct aacgagacgc attccttgac cttgatggga aagttgtagt
    10801 cgatattgat tttttgttcg acataggcaa ttcggtgtaa ggatttttta acttccttgt
    10861 catcgagaaa tgcctgacct tgatgtggga taattcccag catacctttt aatagtgttg
    10921 atttcccagc gccgtttgga ccaatgatgc cggtaattgt tggtccatgg agcactagtg
    10981 aaatatcctt aagtgccaac gtttctttgt aggagacact gaggttttcg atacgtatca
    11041 taaacttgta ttcctcctgt ctcttaatat acattaaaaa aaaaattaag tcaagttaat
    11101 ttttgaaaaa attaaaat
//
LOCUS       DAVKWS010000059        10787 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKWS010000059 DAVKWS010000000
VERSION     DAVKWS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.060"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            1..727
                     /locus_tag="VW852_001752"
                     /pseudo
     CDS             1..727
                     /locus_tag="VW852_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            627..1073
                     /locus_tag="VW852_001753"
     CDS             627..1073
                     /locus_tag="VW852_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0272843.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCPALVSIDYINCLSETTD
                     KNWHKSDRIFVTNIGKPVHSSILSKSLQRANERLKKPIPKHLPPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTQVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1211..2515)
                     /gene="eno"
                     /locus_tag="VW852_001754"
     CDS             complement(1211..2515)
                     /gene="eno"
                     /locus_tag="VW852_001754"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV0272844.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2679..3125
                     /locus_tag="VW852_001755"
     CDS             2679..3125
                     /locus_tag="VW852_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV0272845.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3122..4237)
                     /locus_tag="VW852_001756"
     CDS             complement(3122..4237)
                     /locus_tag="VW852_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV0272846.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENRYKIPEGVQIHILADVVSPLCGYQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4246..4575)
                     /locus_tag="VW852_001757"
     CDS             complement(4246..4575)
                     /locus_tag="VW852_001757"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV0272847.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4520..4891)
                     /locus_tag="VW852_001758"
     CDS             complement(4520..4891)
                     /locus_tag="VW852_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV0272848.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4950..6383)
                     /gene="glgA"
                     /locus_tag="VW852_001759"
     CDS             complement(4950..6383)
                     /gene="glgA"
                     /locus_tag="VW852_001759"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV0272849.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6380..7519)
                     /gene="glgD"
                     /locus_tag="VW852_001760"
     CDS             complement(6380..7519)
                     /gene="glgD"
                     /locus_tag="VW852_001760"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV0272850.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAEMFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GKGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7509..8651)
                     /locus_tag="VW852_001761"
     CDS             complement(7509..8651)
                     /locus_tag="VW852_001761"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV0272851.1"
                     /translation="MKNKMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGAVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8641..10569)
                     /gene="glgB"
                     /locus_tag="VW852_001762"
     CDS             complement(8641..10569)
                     /gene="glgB"
                     /locus_tag="VW852_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV0272852.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFVERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3303 a         2362 c         1912 g         3210 t
ORIGIN      
        1 ctaaaagaga aaacaggaga gctaaaccac caagtaaaag gaacaaaatc aagaaaagtt
       61 gatagagata gacttaaaaa ctatcttact gacaatcccg acgcttattt gactgaaata
      121 gcttctgaat ttggctgtca tccaactacc atccactatg cgctcaaagc tatgggctac
      181 actcgaaaaa aagaaccaca cctactatga acaagagcca gaaaaagtag ccttatttct
      241 taagaatttt aatagtttaa agcacctagc acctgtttag attgatgaaa caggattcga
      301 tacttatttt tatcgagaat atggtcgctc attaaaaggt cagttaataa gaggtaaagt
      361 atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg gtgagttaat
      421 cgttccaatg acttacgaag agacgatgac gagcgacttt tttgaagctt ggtttcagaa
      481 gtttctctta ccaacattaa ccacaccatc ggttattatt atggataatg caagattcca
      541 tagaatgggt aagttagaac ttttatgcga ggagtttggt cataaacttt tacctcttcc
      601 tccctactcg cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct
      661 caaaaaggta ttaccaagtt gcaatacctt ttacgaggct cttttgtcct gctcttgttt
      721 caattgacta tataaattgt ctaagcgaaa caaccgataa gaattggcac aaaagcgacc
      781 gtatttttgt taccaatata ggaaaaccag ttcatagttc tatcttgagc aagtctctcc
      841 agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca tctgccccct cacatcttca
      901 gacacaccac tattagcatc ttatcagaaa ataaaattcc tttaaaaaca atcacggaca
      961 gggttggtca ttccgactct gaagtcacta cttccatcta cacccaggtc acaaagaata
     1021 tgaaagatga agcaatcaat gtactggata aagttatgaa aaagattttt taaaaagttt
     1081 tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg gataaaatcc gaggggctag
     1141 aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg gttcgggata aaatagttca
     1201 ctgaactatt ttatttttta aggttgtaga atgatttcaa tccacgatat tcagctactt
     1261 caccaagttg gtcttcgata cgaagcaatt ggttgtattt agcgatgcgg tctgtacgtg
     1321 aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac tgcaatatca gcgattgttg
     1381 aatcttcagt ttcacctgaa cggtgtgata caacagcagt gtaaccagct tctttagcca
     1441 tttcgatagc ttcaaaagtt tcagtaagag taccgatttg gttaactttg ataaggattg
     1501 agttagcagc accttcttgg ataccacgtg caaggtagtc agtgtttgtt acgaagaagt
     1561 cgtcaccaac aagttgtact ttcttaccaa gacgttcagt aagagctttc caaccatccc
     1621 agtcgttttc atccatacca tcttcaatag tgatgattgg gtatttgtta accaattctt
     1681 caaggtagtc gatttgttct gcagatgtac gaacagcagc accttcacct tcaaatttag
     1741 tgtagtcgta aactttacgt tctttatcgt agaattctga tgaagcacag tcaaatccga
     1801 taaatacgtc tttacctggt acatatccag cagcttcaat cgcagcaagg atagtttcaa
     1861 caccatcttc agttccttcg aaacgaggag cgaatccacc ttcgtcacct acggcagttt
     1921 ccaaaccacg tgatttaagg attttcttaa gagcgtggaa gatttcagca ccgtaacgaa
     1981 gggcttcttt aaatgttggc gcaccaactg gcaagatcat gaactcttgg aaagcgattg
     2041 gagcgtcaga gtgagaacca ccgttgatga tgttcatcat tggagttgga agaactttag
     2101 tgttgaatcc accaagatag ctgtaaagtg ggatttcaag gtagtcagca gcagcacgag
     2161 ctacagcgat agacacaccg aggattgcat tcgcacccaa tttacctttg ttaggagtac
     2221 cgtcaagtgc gatcatagca cggtcaatag cttgttgatc acgtacatcg tagccaatga
     2281 tagcttcagc aatgatgttg tttacgttgt caacagcttt ttgtgtacca agaccaccgt
     2341 aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg ttcaccagta gaagctcctg
     2401 atggaaccat accacgtccg aaagcacctg attcagtgta aacttctact tcaagtgttg
     2461 ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc agtaataatt gacatttttt
     2521 actctcctta tgagttaaat tttttacacc tctataatac cttaaaaccc ctcctttttc
     2581 aagaaaaaac gttatctttg tgcaactttt ccttaacttt ataaagtaat cgctttcttt
     2641 tgtctgtttt attctaactt ttatgatata ctgttttcat gacagattta tcaaaacaat
     2701 tacttgaaaa agctcatggt gggttaaaaa taaatccgga tgagcaaaga cgctatcttg
     2761 gtacttttga ggaaagagtt cttggatatg tagatattga cacagcaaat agccctcagt
     2821 tagaaaaagg ctttttattt attttagaaa accttcagga aaaagcagag ccactatttg
     2881 tgaagatttc accaactatc gaatttgata agcaagtttt ctacttaaaa gaagcaaaag
     2941 aaactgatag tcaagccacc atagtatctg aagagcatat tacttctcct tttggcctgg
     3001 ttattcatag caatgcacca gttcaagtag aagaaaaaga ccttcgactt gcttttccaa
     3061 aactttggga agttaaaaag gaagaaccag ccaaaacatc cttatggaag aaatggttta
     3121 gctaaatctt gcacatattt aataagtgcc caatattggc agccgtgcgc tccagataga
     3181 gactggcatt tttcaaacta tcttctaaag gttcactttt ctccaaaata gaaaagacag
     3241 cttggatatt ttcaaatggt aggggaggta aatcttcagc aagactaccg caaatagcaa
     3301 taacaggaac tccaacaggg gttctttttg caacacctat aggcgctttc ccagcaaagc
     3361 tttgactgtc aagtcttcct tctccaacaa caaccaagtc agcatctgaa actttcttat
     3421 caaagttgat taagtccaag caggtatcaa ttccagacac gatacttgcc tgagcaaagg
     3481 cacacaaacc accagcaagg cctccacctg ctcctgctcc tttaatttct aatgttgcag
     3541 gtgagaattt ttcataaaaa tcttggatcg cctgatctac gactgcaaac atagtcggat
     3601 gtagaccttt ttgattgcca aaagtgtaag tcgcaccttg ataaccacat aagggactca
     3661 cgacatctgc taaaatatga atttgaacac cttcaggaat tttatagcga ttttctgttg
     3721 aaacagaagc taagtttaat aaggattgac cggaagcagg caagacattt ccatccctat
     3781 cataaaattg ataacctaaa ccagcagcaa tccccagtcc tccatcatta ctggccgtgc
     3841 caccaacacc gatataaata tctttaatcc ctttagaaat gagatggaga atcaactctc
     3901 caataccaca agtttggatt tgaagtggat ttcgtttctc tagcggaatt tttccaagac
     3961 caaccaagtc agctacttca aatagtgcca gttccccttt ttgaaaatag cgcatggctt
     4021 ctttttgtcc aaaagggtct gtcacttgga tccatttttc ttttaggtca agagaatgtc
     4081 ggatagcatc tacagtacct tctcccccat caccaacagg gcagaggaga cattctacat
     4141 ctgctatcga ttgttggaag cctcttttta ttgcttcagc tacctgttga gctgtcaagc
     4201 tttccttaaa cgaatccggt gcaattacaa tcttcatatt ttccctcatt ctaaacagtc
     4261 aatcaaagga agaacttcta aaaaatccct cttgtcaaca tgatgtggta tttctttttt
     4321 gagcacttct ttggcacaaa aggcgattcc taactccgcc gacttcaaca ttaatagatt
     4381 attgacccca tcaccgattg ccaccgttct ttctttagaa agttttagtt tctttctcca
     4441 tttttccaga gtttcttttt tgacctgggg acttataatt tgtccaacta attttcctgt
     4501 taaaagacct tctttgactt caagctggtt ggcagtgaaa taggcaatac caagggattt
     4561 tgctaatctc tccaactatt ggtgtaaatc caccagacac cagaccaact aggatgccat
     4621 tcttttggag aatagagatg aactctggga catttagcga tagatgaatt gagttgaaga
     4681 cgttatcaaa gaccaaaata ggaagacctt ccaacaagga cactcttttt cttaaactgc
     4741 tttcaaagac caactctcct cgcattgctc gacttgtaat ctgcgaaatt tccgcctcat
     4801 gacctgcctc tctccctaaa agatcaatca cttcttctag gattaaggtt ccatctacat
     4861 ccaaaacaca caagcctttt acttgagaca tcagttctcc tctctaaaca gcctaaaaat
     4921 cgtatgaagt catcatacga ttttatctat taattaacta aactatggta caagtcaagg
     4981 tatgacttgc aggctgtatc ccatgagaag tcactctcca tagcttgttt ttgtaggttt
     5041 ctccaaatgt ctggatggtt tctatacaag tccaatgctg tttggaaagt ccaatttaac
     5101 caataaggag atagattgtc aaagctaaag ccagtaccgc ttccttcgat tggattgaaa
     5161 gcgcgaactg tatctcgcaa gcctccaact tcatggacca atggcaaggt tccataacgc
     5221 atagccatca tttgagacaa gccacacggt tcaaaacgac ttggcatgag gaagaggtca
     5281 caagcagcgt agatttcctg agcaagtttg acatcaaaag tgatatttgt tgatagcttg
     5341 tctgggtaaa tctgagcaaa ccatgagaaa gctccttcaa aggctggatc gccagttccc
     5401 aaaagaacaa tctgaacatc ttcttgcaag atatggtgaa gactttcgac caccacatca
     5461 aaaccttttt gacgtgtcaa acgagaaaca attcccacca gtggaacgtc tgctctaaca
     5521 ggcaagccaa ctctttcttg caattttgcc ttatttttgg ctttcccaga caaatcttcc
     5581 tgattgaaat gatagtctaa aagagcatcc gtctgaggat tataaagatc agcatcaatc
     5641 ccattcacga taccagatac tttaccagac tccattttaa gaatctgatc caaattacat
     5701 ccaaactgac tagtcataat ttcatgagca tagctaggtg aaacggttga aacacggttc
     5761 gcatagagaa tacctgcctt catccagttc agacagttgt tccatcgaag ggtgccatca
     5821 gcgtaacgtt caaggccaac tccaaacaaa tcacccaaca ttccttctga aaattgtcct
     5881 tggaattcta aattatgaat ggttaaaact gtttcaatgt cctcataggc ttgaatccaa
     5941 cggtattttt ccttcaacaa gaaaggaatc atagctgtat ggtagtcatg aacatggaga
     6001 agatcaggaa taaagtcaat cctttccata gcctcaatgg cagccagttg gaaaaaggca
     6061 aagcgttctc cgtcatcaaa atcaccgtaa acatgaccac ggaagaaata atattgattg
     6121 tcaataaagt agaaggttac accatttaat actgttttct taattccaca atactgtctg
     6181 cgccaaccaa cgctcacctc aaaatgaagc acatcttcaa tctgatttcc aaatttagcc
     6241 tctaccatat catagtaggg taaaatcact gcaacttcgt gcccagcttt taccagtgat
     6301 tttggaagag cgccaatgac gtctcccaaa ccacctgttt ttgaaaaagg tgcaccctct
     6361 gctgctacaa ataaaatttt catgaatgaa tatcctctgt tactttagca cctttcttaa
     6421 ccacaactgg atgttctgca gttcctcgaa tcacaacacc aggctcaact tcaacccctt
     6481 tgtccaagat agcatattcg acctgagccc cttttccaat aacaacacga gggaagagca
     6541 ggctatcttt aaccaagcta tccttatgga catgaatatt acgtgataga acagaattag
     6601 ctacttgacc ttcaataata ctaccagagg caaactgaga agtgcttacc ttagatgtat
     6661 tagcatagta agttggctct tcgtttttga cctttgtata aatcttttgg tttggtgaga
     6721 aaagagaata gaatttttgt gattcaagca tatcgatatt cgcttgataa taagatttaa
     6781 cagagtgaat attggctaga tagcccgtgt actcgtaggc gaaagctccc tcttttacag
     6841 ccaaatcccg taaaacatag cgcaatttct ttggatgttc ttttttagct tcttcttcca
     6901 agtgttcaat caaccaaggt gtatcaacga caaagatatc tgtagacata ttgaacattt
     6961 cagctgttga cttgctatca aagagtttat gagaaagaac atggtctgtt tcatctacat
     7021 ccaagattgc atttacttct gaaatatctt tcttagctag ttttttataa actacagtga
     7081 taggctcttt tgttgtacta tgtaggtgga aaacttggtt caaatcaatg ttaataagaa
     7141 catcgcagtt gagggcaacc gtttggtttg agccagaacg tttcaaataa gtaagaagct
     7201 gttggtagta ttcttttcca actgtactac tttctacacg ggtattgtaa attcctagat
     7261 agtaatgact aagaagggtt gataagcccc actcgcgtcc tgaacgaata tggtcaaata
     7321 ctgagctgat attatcctgc tggaaaatac caaagacact acgaacacct gcattagcaa
     7381 ggcttgaaag tgggaagtca atcaaacgat atttcccacc aaatggcaaa cttgctactg
     7441 gacggtggtc cgtcaatgtc gacatattgt gaaaaccaac tgtatttcct aaaatggcag
     7501 aatatttatc aatcttcatc tgttgctacc cccactactt cattatatcc tacaacttgt
     7561 acttcatctg ttccatcaat ttcgacaccg tcagaaataa tcgcaccttc accaataatg
     7621 gcacgtttaa tcttagctcc ttgaccaatg atagctccac tcatgataac tgaatcaagg
     7681 acttccgctc cttcgcgaac ttgcgcgcct gttgaaagga tagaatgttt aacagctcca
     7741 tcaacgaaac atccgtctac aactaatgag tcttccacat gagcatttgc cccgaggaag
     7801 tttggtggtg aaatcaagtt tcttgagtaa atcttccatt gacggttacg actatccaag
     7861 gcattttctg gagaaatata ctccatgttc gcttcccaaa gtgactcaat agtaccaaca
     7921 tctttccaat aaccactaaa ttcgtaagca taaacacttt cacctgactc aaggtaattt
     7981 ggaatgacat ttttaccaaa gtctgacatg ccaaccttgc tcttttcagc agcgactaac
     8041 atattacgaa ggcgttgcca atcaaaaatg tagattccca tagaagcttt tgtagattta
     8101 ggttgagctg gtttttcttc aaattcaaca atacgattgt tagcatctgt gttcatgata
     8161 ccaaaacggc ttgcttcttt aagagggacg tccaaaactg ctactgtcaa gctggcatta
     8221 ttatccttat gagactggag catatcatca tagtccattt tgtagatgtg gtccccagac
     8281 aaaatcaaga catactcagg attgacactg tcgatatagt cgatattttg gtaaatagcg
     8341 tgactagttc cctcaaacca acgatttcct tcacttgcag aataaggttg aagaatagag
     8401 acgcctgaat taataccgtc tagtccccag cttgaaccat tcccaatatg gttgttgaga
     8461 gcaagtggtt gatactgtgt aacgacccca acattatgaa tccctgagtt ggcacagttt
     8521 gatagggcaa agtcaatgat acggtagcgc ccaccaaatt gcacagctgg ttttgcgatg
     8581 ctttgagtga gtttaccgag acgagttcct tgcccaccag caagaatcaa agctaacatt
     8641 ttatttttca ttttctactc ctttttggtt tttatttgtg acggttttag tagatttcaa
     8701 gcgacgtttg attttccata cacttgctcc catagccggt agagtaaagg ttaaggtctg
     8761 ctcataatct ttccataggc cttcttgcgt ttgaacagtt tgattatgtt ctttccaaac
     8821 gcctccccac tcttccaact cagtattcca tacttcttcg taaattcctg caacgggtag
     8881 tccgattgta aaatctttcc gctcaacagg taccatgtta aagatacaga ctaacatttc
     8941 tcccttttta cccttacgaa taaaggaaag aacactctgg tctcgattat ccgcatcaat
     9001 gatttcaata ccatcatagc tggtatcgat ttcccacaga cagcgatgat ctttgtaaaa
     9061 ctggtttagc tgagaagtga aatacttcat cttagcattc attgggtctt ctaggttaga
     9121 ccattccaac tgttcttcag atttccattc taggaattga ccgtattcgc tacccatgaa
     9181 gagcaatttc ttaccagggt gacaaatttg gtacgtatag agattgcgca agcctgcgaa
     9241 ttgattgtaa cgatctcccc acatcttatg catcatactc ttcttgccat gaaccacttc
     9301 atcgtgcgag aatggcaaga gataattctc cttgaaaaca tacataaagc tgaaagtcac
     9361 caggttaaag tcatatttac gataaatcgg atcttcttcg tagaaacgga ggatatcatt
     9421 catccagccc atgttccatt tgtagtcaaa tcctagacca ccaatctctt tcattcccgt
     9481 aatcttggtc gcagacgaac tttcttctgc aatcatcatc acatctggat attctaactt
     9541 aataacttca ttcaagcgtt gaagaaaata ataaccttca tagttaagat ttccgccatc
     9601 tttattaggt gtccatggag cattatcata gtccaaatag agcatgttgc taacagcatc
     9661 cacacgaata ccatccaaat gatagacatc aatccaatgc ttaatgcaag aaattaagaa
     9721 ggactggact tcattttttc caaggtcaaa attaagggca ccccaaccat ggttatgagc
     9781 cttattatgg tcttggtatt caaaagtcgg tgtcccatca taataggcta aggcatcatc
     9841 gttgatggta aagtgacctg gtacccagtc cacaataacc ccaatattat gggtatgaca
     9901 ctcctcgaca aaatcttgaa actcctctgg tctgccataa gcatgctcta aagcgaagta
     9961 acccataagc tgataccccc aactcaagcc caaaggatgg gacatcaagg gcataaactc
    10021 aatatgagta tagttcattt caacgagata aggaatgagt tcatccttga gctgggcaaa
    10081 actataagga ctgccatcag gatttctctt ccaagagcca gcatgaacct cgtaaatatt
    10141 gacaggacgt tcaacaaagc cccaacgttt tcttcgtgcc agccaaagtc catccctcca
    10201 tttcttatca ggaagctctg ttacgattgc ccctgttcct ggacgagcct catacctgac
    10261 agcaaaaggg tcaatcttca tcagttgatg accattttga cgtgtgacat gatatttgta
    10321 aatatgccct tcttgagcca tattggtaaa gacttcccag accccaaagt catttcttac
    10381 cattggaatc tgattttcaa tccagttggt aaaatcacca accaagtgaa cagcctgagc
    10441 attaggtgcc caaacacgga aggtatagcc atgctctcca tttagttctt ccctatgtgc
    10501 tcctagataa tgttggagat aaaaattttc acccgtcata aaggttttta atgcttctct
    10561 attatccata tactcccctt ctcctgtaag cgttttctat gtttttatta tactaccttt
    10621 ttagagaaga ttcaagtaaa ttactatact tctttaatta ttttgaaaat ctacaacaag
    10681 ttcacttact cgttcaattg taaatcaata ttttttcaaa aaattgcgaa aacgcctttc
    10741 tttttctact atagtgaaat gaaataaaac atgcgcaaat cgatcag
//
LOCUS       DAVKWS010000060        10736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKWS010000060 DAVKWS010000000
VERSION     DAVKWS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.061"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            364..1242
                     /gene="truB"
                     /locus_tag="VW852_001763"
     CDS             364..1242
                     /gene="truB"
                     /locus_tag="VW852_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV0272853.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            1483..1785
                     /locus_tag="VW852_001764"
     CDS             1483..1785
                     /locus_tag="VW852_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0272854.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            1893..2531
                     /gene="udk"
                     /locus_tag="VW852_001765"
     CDS             1893..2531
                     /gene="udk"
                     /locus_tag="VW852_001765"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV0272855.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            2659..3999
                     /locus_tag="VW852_001766"
     CDS             2659..3999
                     /locus_tag="VW852_001766"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV0272856.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEVKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            3977..4189
                     /locus_tag="VW852_001767"
     CDS             3977..4189
                     /locus_tag="VW852_001767"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV0272857.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            4186..5061
                     /locus_tag="VW852_001768"
     CDS             4186..5061
                     /locus_tag="VW852_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV0272858.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQIPFE
                     TESIVSVVESLRING"
     gene            5054..5869
                     /locus_tag="VW852_001769"
     CDS             5054..5869
                     /locus_tag="VW852_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV0272859.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            5862..6293
                     /locus_tag="VW852_001770"
     CDS             5862..6293
                     /locus_tag="VW852_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0272860.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            6300..7967
                     /gene="recN"
                     /locus_tag="VW852_001771"
     CDS             6300..7967
                     /gene="recN"
                     /locus_tag="VW852_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV0272861.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            7969..8697
                     /locus_tag="VW852_001772"
     CDS             7969..8697
                     /locus_tag="VW852_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV0272862.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDYPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            8776..10599
                     /gene="lepA"
                     /locus_tag="VW852_001773"
     CDS             8776..10599
                     /gene="lepA"
                     /locus_tag="VW852_001773"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV0272863.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3188 a         1924 c         2609 g         3015 t
ORIGIN      
        1 ataaccagaa attatcttta cgagggatga attataaatt gaatcatgaa gaacttaaca
       61 attttgattt gttgaaaaaa atgtttctga aattaatgga gaccgataaa atagagaatg
      121 atatttttta tttagatggc atcaaaagta tactcccatt attatcttta gatgacagac
      181 gaaatgtatt acaacatatt caaaaacaga acaattgtcc tcctccagag attgaggaat
      241 tacaaagaat tatagttaat taaattttgt aaaaaagaga ataaaaggta gacgacgcct
      301 cagtaaaaat tggcccggaa aaaagcgaag ttcgaagcac tgaaggggga gtagaaagca
      361 aaaatgaacg gtattatcaa cttaaaaaaa gaagcaggaa tgacctcgca tgatgcggtt
      421 tttaaactgc gtaagatttt gggaaccaag aaaattggtc atggtggaac cttggatccg
      481 gatgtggtgg gtgttttgcc gattgcggtt ggcaaggcga cacgcatggt cgagtttatg
      541 caggacgagg gtaagatcta tgagggggaa attactctgg gttattccac gacgactgag
      601 gatgctagtg gggaagtgat cgcagaaacc cctgttttgt ctcccttgga tgaaaagctt
      661 gttgatgaag cgattgctag cttgactggg cctattaccc agattccccc tatgtattcg
      721 gcagttaagg ttaatggtcg caagctctat gagtatgcgc gtgctggtca ggaagtggag
      781 cgtccagaac gtcaggtgac catttatcaa tttgagcgaa caagtccgat ttcttatgat
      841 ggccaacttg ctcgattcac ttttcgtgta aaatgcagta aagggactta catccgtact
      901 ttgtcagttg atttgggtga aaagcttggt tatgcggctc atatgtccca tttgactcgt
      961 actagtgctg ctggcttaca attagaagac gctcttgcct tggaggaaat tgctgaaaaa
     1021 gtagaggctg ggcaattaga ttttctccat cctttagaga ttgggacagg tgaccttgtc
     1081 aaagttttcc taactccaga agaggctaca gaagttcgct ttggtcgttt tattgagcta
     1141 gaccaaacgg acaaagaact ggctgccttt gaagatgata aattgttagc cattctagaa
     1201 aaacggggca atctctataa gccaaggaag gtttttagct agatcgttta ggaataaaaa
     1261 tcgggtgata gataacaatt gcttgataaa accccatact aatagtagaa tggttttggg
     1321 aattataata ttccaattgt tgcgagttgt aggtactcaa ataatctata tagaaattta
     1381 gaggtgtgaa atgaagcaat ttaaaattct ttcagataaa tatttagagt ccattacagg
     1441 ttctggtggg aacttaggcc caggatttgg tgtgataatt ccatgatgcg aaatgagttt
     1501 cgagaaaggg tggagcaact tcttcaacaa aaagaaataa atgaaaatag tgagttgagt
     1561 cacctgtttc gtcttgctat acaaaattta gacagaaatg aaaaatacca atcggtcatg
     1621 gccaatttga gtcaagggtt gtcactttac ctcatgacgc atcattacca ggcacctaag
     1681 tctgtcattg attttggttt atggattgcc aaagctccta gccaggaaag agggagactg
     1741 gctttcttgc agatgcttgc tcaaacccta cagggctttc gttaaaactg tcgaacatca
     1801 gagggaatac tctggtgttt tttgctgatg agactataaa ttttgtaaaa aatgtgatag
     1861 aatagacgac ggataaaata acggaggata gcatgcaaaa tagaccaatc attattggag
     1921 tgacaggtgg ttctggtggt ggtaaaacca gtgtttcaag agccatttta tcgcattttc
     1981 ctgatgaaaa gatttccatg attgagcatg attcatacta caaggatcag tctcatttga
     2041 cctttgaaga gcgtgtcaaa accaactatg accatccttt tgcctttgat acagacttga
     2101 tgatcgagca gattaaggaa ttgttggcag ggcgtccggt ggacatcccg acctacgact
     2161 atacagagca tacacggagt agcaagacct atcgtcagga gcctcaagat gtctttatcg
     2221 ttgagggcat tttggtcttg gaggacaagc gtctgcgcga tttgatggat atcaagattt
     2281 ttgtggatac ggatgacgat gtgcgcatta ttcgtcgtat caagcgtgat atggaggagc
     2341 gtggccgtag ccttgatagc gttattaacc agtacttagg tgtggtcaaa ccaatgtacc
     2401 accagtttat cgagtcaact aagcgttatg ctgatatcgt cattcctgaa ggggttagca
     2461 ataccgtggc tatcgacctg ttgacgacca agattgcaaa gattttggaa gaagctcgaa
     2521 acagcaaata atcacatgtg gaggccttgc ctcctttttc tatttttccc tcataatggt
     2581 acataaatgg agatttttag gcatattttt ggtataataa tacccatgaa agagcaagta
     2641 aatgaatagt aggtggagat ggaaaagtat ttatcggtaa caactttgac caagtatctg
     2701 aaaatgaaat tcgataaaga cccatacttg gaacgggtct atttaactgg tcaagtttcc
     2761 aactttcgta aacgacctac tcaccaatat ttctccctaa aggatgacca tgcagttatt
     2821 caagcgacca tctggtctgg gatttatcag aaattagggt ttgacctgga agaaggaatg
     2881 aagatcaatg tgattgggcg tgtacaggtt tatgaaccaa gtggtagcta ctccatcatc
     2941 attgaaaaag ctgagcctga tggggttggg gcgcttgcga ttcagtttga acaacttaag
     3001 aaaaaattga cagaagaagg cctgtttcaa gaacgcttca agcaagctct gccccaattt
     3061 tctaagagaa ttggggtagt aaccagccgt agtggagccg ttattcgaga tattatcacg
     3121 accgtcagca ggcgattccc aggtgttgac atccttcttt atccgaccaa ggttcaaggt
     3181 gaaggggctg cggaggaaat tgctagaaat attgcgcgtg ctaatcaacg ggatgatttg
     3241 gatttgctca ttattggtcg tggaggtggt tctattgagg atctctgggc ctttaacgaa
     3301 gaaattgtgg tacgagctat ttttgaatct cgtttgccag ttatctctag tgtggggcat
     3361 gagacggatg tgaccttggc agattttgtg gcagatcgac gcgctgcaac gccaacagcg
     3421 gcggctgaac tggcaacacc tgtgaccaag ttggatgtat tagctcattt gcaaaatcag
     3481 gaaaaacgga tggtaacggc agtccgaaat gttctatcta agaaacaaga ggctttgaaa
     3541 aaatgcagtc agtctgttat ctttagacaa cctgagcgct tgtatgacgg ttatttgcaa
     3601 cgcttggacc aactgcaact gcgtttgaaa caaagtttgc gaactcggat ttctgataac
     3661 aaacaattag ttcaagcaag aactcatcaa ttagtacaat tatcacctgt taccaaaatc
     3721 caacgctatc aagaccgttt aggacagttg gacaagctct taggtagcca aatggcgtta
     3781 gtttatgacg ccaaggttgc tgaggtcaag cgactttcgg aagctttgct catgttggat
     3841 actagccgaa tcgtggcgcg tggttatgct attgtcaaaa aagaagagtc cgttgtagat
     3901 tcggttgaga gtttgaagaa aaaagaccaa gtaacgcttt tgatgcgaga tggtcaagta
     3961 gaattagagg ttaaagatgt caaaacaaaa gaaatttgag gaaaatctag cagaactgga
     4021 aaccattgtc caaagtttgg aaaatggtga aattgctctg gaagatgcga ttactgcctt
     4081 tcaaaagggc atggtcttgt caaaagagct ccaagctacg ctggacaagg ctgaaaagac
     4141 cttggtcaag gtcatgcaag aagacggaac agaaagtgat tttgaatgaa aaagcaagaa
     4201 aaattagctc ttgtcgagtc ggctttggaa gatttttatg gagaccagca gtttgcctct
     4261 agtttacggg agtctgttct ctattctatt catgctggtg gcaagcgtat tcggcctttt
     4321 ctcttgttag aagttctgga agccttgcag gttaccatca aacctgctca cgcgcaggta
     4381 gctactgcct tggagatgat tcatacaggg agcttgattc acgatgacct tcctgctatg
     4441 gatgatgacg attatcgaag agggcggtta accaatcaca agaaattcgg tgaagctatg
     4501 gccattttgg ctggagatgc cttattctta gactcatatg ccttgattgc gcaggcagat
     4561 ttgccaagtc agattaaggt ggacttgatt gccaacttat cccttgcttc aggtagtctg
     4621 ggtatggtgg cagggcaagt tttggatatg gagggcgaac accagcactt gtctttggaa
     4681 gaacttcaga ctattcatgc caataagact gggaagttac tagcctatcc cttccaagcg
     4741 gcagctatta tagctgaatt gtcacctgaa atgcaggtga agctgaaaac tgtgggtgaa
     4801 ttgattggac ttgcttttca agttagagat gatgtactgg atgtgacagc tagttttgag
     4861 gaaatcggca agacacctca aaaggatctg caggcagaaa aatcaaccta tcctgccctg
     4921 ttgggcttgg aagagtccat tgccttttgt aaccaaaccc tggatcaagc taatgaaaaa
     4981 ttagaagaaa ttgcccagca gattcccttt gaaacagaat cgattgtaag tgtagtagaa
     5041 agtttgagaa tcaatggcta aggaaagagt ggatgtacta gcttataaac agggcttgtt
     5101 tgaaacgaga gagcaggcca agcgaggtgt gatggctggc ctagtcgtag cagtccttaa
     5161 tggagaacgg tttgacaagc caggagagaa aattccagat gacaccgaat taaaactcaa
     5221 gggggagaaa ctcaagtatg tcagccgtgg tggtttgaaa ctggaaaagg ccttgcaggt
     5281 ctttgatttg tcggtggatg gcgcgactac gattgatatc ggggcctcta ctggaggttt
     5341 taccgatgtc atgctacaga atagtgccaa gttggtcttt gcagtcgatg ttggtaccaa
     5401 tcagttggct tggaaattac gccaagaccc acgagttgtc agtatggagc agttcaattt
     5461 ccgctatgct gaaaagactg atttcgagca ggagccgagc tttgccagta ttgatgtgag
     5521 tttcatttcc cttagtctga ttttgccagc cttgcaccgt gtcttggctg atcaaggtca
     5581 ggtggtagca cttgtcaaac ctcagtttga ggcaggacgt gagcagattg ggaaaaatgg
     5641 aattattcga gatgctaagg ttcatcagaa tgtccttgaa tctgtaacag ctatggcagt
     5701 agaggtaggt ttttcagtcc ttggcttgga cttttctccc atccaaggtg gacatggaaa
     5761 tattgaattt ttagcgtatt tgaaaaaaga aaagtcagca agcaatcaga ttcttgctga
     5821 gattaaagaa gcagtagaga gggcgcatag tcaatttaaa aatgaataaa aaagagagac
     5881 ttgaaaaaat tagaagattg gtgacagatt atcaaatcgg cacgcaagaa gaaattgtag
     5941 aacatttgaa agaagcaggt atcactgcca ctcaggcgac ggtatcccga gatatcaaag
     6001 agttaggtat tgtcaaaatt cctttgagag acaacaccta tgtctacgaa ttgccaaaat
     6061 caatcgtaaa aagtctgcaa ctggctgaag acaatatcga atcggctgaa ttgatggata
     6121 agatgatcaa tctccaagtt attccaggaa atacggcttt tgtaaaagct cagttaatcg
     6181 atacttttgc agacaagatt tttagttgtt tgactgatga tagctcgatt ttagtcattg
     6241 ccagaagtgg aagtctagca gaggaaatct ttgaacaagt aaaaaattgg taggtctata
     6301 tgttacttga aatttcgata aaaaactttg ccattattga ggctatttcc ctcaattttg
     6361 aaaaggggat gactgtcctg actggtgaaa cgggtgcagg gaagtcgatt atcattgatg
     6421 ccatgaatat gatgttggga gctcgtgcga cgacagatgt tattcgtcat ggtgcaccaa
     6481 aggcagagat tgaggggctt ttctcagttg agaatagtcg tcttttacag gaaatttttg
     6541 atgagcaagg tttagagtta ggtgatgaaa ttatcatccg tcgggaaatc ttgcaaaatg
     6601 gtcgtagtat cagccgtgta aatggtcaga tggttaatct gtctgttttg cgagctattg
     6661 ggcaacatct tgtagatatt catggtcagc atgaccatga ggagttaatg cgtccccaac
     6721 tgcatatcca gatgttggat gaatttggtg atgccgcttt ttgggacttg aaagaaacct
     6781 atcaaacgag ttttgatgcc tatcggaaaa tgcgcaagca ggttctggaa gtcaagaaaa
     6841 accaacagga acacaaggca cgtatcgaaa tgttagaatt tcaaatggca gagattgagg
     6901 cagcaaactt gcaggctgga gaagacttgg atctcaatca agagcgagat aaactcctca
     6961 accacaaaaa tattgcggat acactgacca atgcctacag tatgttggac aatgaagatt
     7021 tttcaagtct ggccaatgtt cgttcagcca tgaatgacat ggaaagtgtc gaagagtatg
     7081 accctgaata ccgtgaaatt tcaagctctc tgtctgagac ctactatgtt ttagaagaca
     7141 ttagcaaacg tttggaagct atcattgagg accttgattt tgatggcaat cgcctcatgc
     7201 aggttgagaa tcgtttggac ctccttcata ctattactcg taagtatggt gggactgttg
     7261 atgatgtttt gctttatttt gccaagatta cggaagaata caatctcttg acaggcaata
     7321 atctttcgtc tgaggacatg gaagcagagc ttaagaagtt ggaagtcaat cttgtcaatt
     7381 tggcaggtca acttgcttct gctcgtcata atttggctca gcaactcgaa gctgagatta
     7441 aacaagaact gcaagatctt tatatggaaa aagcccagtt tcaggttcgt tttagtaagg
     7501 gaaaattcag tcgtgaggga aatgaaatgg ttgagtttta tatttcaacc aaccctggag
     7561 aagactttaa acccttggtt aaggttgctt ctggagggga attatctcgt ctcatgttag
     7621 ccattaagtc tgccttttca cgtaaagaag gcaagactag cattgtcttt gatgaggtgg
     7681 atacgggagt ttcaggtcgt gttgctcaag ctattgctca gaaaattcat aaaattggtc
     7741 agtatggtca ggttttggct atctcccatt tgccacaagt aattgcgatt gcagattatc
     7801 aattctttat tgagaagatt agtaatgacc attcaacggt ttcgactgtt cgtctcttga
     7861 cagtcgaaga gcgagtggag gaagttgcca agatgttggc aggtgatgat gtgacagaag
     7921 cagccctgac gcaagccaga gaattgttga gaaacaggga gaaataagat gacagattat
     7981 tatgtaattg gagatgttca cggaaaagct gggatgctgg aagaccttct taaaacctgg
     8041 gatggtcaga cccagttgct ctttctaggt gatttgattg acagaggtga ggatagtcac
     8101 cgtgttcttg aaatggtcaa ggacttggtg gacaatcaag gggctatctg tttgtctgga
     8161 aaccacgagt atatgttttt gacttggcta gattacccag aagaaagtta tgaccattat
     8221 cgtcgcaatg gtggagatac aaccattaac tctatcctag gtcgtccctt ggatgcacca
     8281 gttgatggag tagaagatgc caagcgggtt gccgctgaag cggcagactt ggtcgaattc
     8341 attcgtcaaa tgccatttgt agtagagaca gacaagtata tctttgttca cgcaggtatt
     8401 gatttgacct tggacgactg gcatgaaaca acagattata aaaaagtatg gcttagaaaa
     8461 ccattccacg gagctgaaaa tcatactgga aaaaccattg tcttcggtca tacaccggtc
     8521 tatggtttgt tacagcaaga acgcggtaca tctgagcttt ggactacaga agatggcaag
     8581 attggaatgg atggaggagc tgtttatggt ggtgtccttc acgggattgt ctttacagac
     8641 caaggaatga cagaacacca ctttatcgaa aatgacggct ttgtcgctga agattagtac
     8701 tcctagcagg gtatggtctt gtcaaaatat caaaaacaat ttataataaa tagataccct
     8761 gaaaggaaga gaatcatgaa cttagaagaa ttgaaaaaac gacaggagaa gatccgtaac
     8821 ttctctatta tcgcccatat tgaccacgga aaatcaactc tagcagaccg cattttggaa
     8881 aaaacagaga cggtttcaag tcgtgaaatg caggcccagc ttttggatag catggatcta
     8941 gagcgggaac gtggaattac tattaagttg aatgccatcg agttgaatta cactgcaaaa
     9001 gatggggaaa cttatatttt ccacttgatt gacacaccag ggcacgttga ctttacctat
     9061 gaagtttcac gttcgctagc tgcctgtgag ggtgctattt tggtggtcga tgcggctcaa
     9121 ggaattgagg ctcaaactct tgccaacgtt tatctagctt tggataatga tttggaaatc
     9181 atgcctatca ttaataaaat tgacctgccg gctgcagatc cggagcgcgt gcgtacagag
     9241 attgaagatg tgattggttt ggatgccagt gaagcagttt tggcttctgc caaggctggt
     9301 attgggattg aagaaatcct cgagcaaatt gtagaaaaag taccagcacc aacgggtgat
     9361 gtgacggcgc cacttaaggc cttgattttc gactctgttt acgatgctta ccgtggggtt
     9421 atcctacaag tgcgtgtcat ggacggagtg gtcaaacctg gtgataagat tcagctcatg
     9481 agcaatagta agacctttga tgtggccgaa gtcggtattt ttacaccaaa agcggttggt
     9541 cgtgatttcc ttgcgactgg tgatgttggt tacattgcgg cgtctatcaa gacggttcag
     9601 gatactcgtg tgggtgatac cgttaccttg gcaaccaatc ctgcggcaga accattacat
     9661 ggttataagc agatgaatcc tatggtcttt gcgggtctct acccaatcga atcaaacaag
     9721 tacaatgacc tgcgtgaagc gcttgaaaaa ttgcaactga atgatgctag tcttcagttt
     9781 gaaccagaaa catctcaggc acttggattt ggtttccgtt gtggatttct tggacttctc
     9841 catatggatg ttatccagga acgtttagag cgtgagttca atattgacct catcatgaca
     9901 gctccgtctg ttatttacaa agttaatttg accgacggtg agtctatgga tgtgtctaac
     9961 ccatctgagt ttccagaccc aactaagatt gcgaccattg aagagcctta tgtcaaggcg
    10021 caaatcatgg taccacagga gttcgtcgga gcagtaatgg agctagctca gcgtaagcgt
    10081 ggggactttg tgactatgga ctatattgat gataaccgtg tcaatgttat ctatcaaatt
    10141 cctcttgctg aaattgtctt tgacttcttt gataaactta agtcttcaac acgtggttat
    10201 gcaagctttg actacgaatt gtcagaatat cgcccatcta agctggtgaa aatggatatc
    10261 cttctcaatg gagataaggt ggatgctctc agctttatcg ttcacaagga ctttgcctac
    10321 gaacgtggga aactcatcgt tgataaactc aagaaaatca tccctcgcca acaatttgaa
    10381 gttccaatcc aagcggctat tggacacaag attgtcgctc gtactgatat caaggccctt
    10441 cgtaagaacg tacttgctaa atgttatggt ggtgacgttt ctcgtaagcg taaactcctt
    10501 gaaaaacaaa aagctggtaa gaaacgcatg aaatccatcg gatcagttga agttccgcaa
    10561 gaagccttcc tcagcgtctt gagtatggat gaagaataag ttatcagaaa tgcctttcct
    10621 tgttcacagg aaatttatat aagaatcaat agtaaagcag gcttagatgg tctgcttttt
    10681 tagtttttat actcttcgaa aatctcttca aaccacgtca tcgtcgcctt accgta
//
LOCUS       DAVKWS010000061        10701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKWS010000061 DAVKWS010000000
VERSION     DAVKWS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.062"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(117..1541)
                     /locus_tag="VW852_001774"
     CDS             complement(117..1541)
                     /locus_tag="VW852_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0272864.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW852_001775"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW852_001775"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV0272865.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW852_001776"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW852_001776"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV0272866.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="VW852_001777"
     CDS             complement(6528..7706)
                     /locus_tag="VW852_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0272867.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="VW852_001778"
     CDS             complement(7786..8649)
                     /locus_tag="VW852_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0272868.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="VW852_001779"
     CDS             complement(8652..10553)
                     /locus_tag="VW852_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0272869.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3211 a         2352 c         1917 g         3221 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag gagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaatagggg ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa ttttttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaagcttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacatcaagg cgagactaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagtcaaacc aatttctcac
     1801 aatgttttag aaacaagggt gtgctattcc aatttcaggc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag accccctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt accaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaactac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggcag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtataggt
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggtaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatgt gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagtc ttacctacca aatcttcttc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatgatca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgtcaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaac actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 atacagattt tcaattgaat ggaaatattg aagtaaaagc tgactagcct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 agcagggaac ttgtaggcta ctgcccactt tggagcctta acggtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcacttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgaaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 acggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtacactt ggcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctctcccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat ggaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgatc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat atggagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaggc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa tcatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgagaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttgg gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgtatggcc tcttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatacctt tgtcaataat
     7981 cttctctgta taagcctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaatcaaaac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagag
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taactcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa aggggagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttgtgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatc tttacacctg atctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgcttt cttctcaaca tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa aaagcaaggt accatcaaag tcaatcaaag cattttccaa cacttccttg
     9121 ctatcaatat ccaagtggtt ggtcggctcg tcaaggatca ggaagttgtt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggct ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc acctttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9481 acgatagcga cagcattcat cttacgaaga tctaggttga taggttgtga caagacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttagtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtaacta gcttttgctc cttcaattcg acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat gcttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggcg
     9961 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tggcaagtgc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cacctgcttg gcgaaaattc tcagataagc ggtcataatc tgacatcagt
    10201 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgtctc
    10261 tccgtccgac gcaaattatt aaagacatga agcatttcat cgtagatggt attttcagac
    10321 tcaaaacggc tatcttgggc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccgctagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat cataagagta
    10561 gtatagcatg tttccctaag gcattcaaga taatcgtaag tcttttagta caacttttat
    10621 aacataaaat aaactaaatt atgtatattt tatattagat tcttcactat cttgttggat
    10681 tttctaacca gctaatcttg t
//
LOCUS       DAVKWS010000062        10537 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKWS010000062 DAVKWS010000000
VERSION     DAVKWS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.063"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            158..937
                     /locus_tag="VW852_001780"
     CDS             158..937
                     /locus_tag="VW852_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272870.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYTF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            939..1121
                     /locus_tag="VW852_001781"
                     /pseudo
     CDS             939..1121
                     /locus_tag="VW852_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1478..1552)
                     /locus_tag="VW852_001782"
                     /pseudo
     CDS             complement(1478..1552)
                     /locus_tag="VW852_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1583..1816
                     /locus_tag="VW852_001783"
     CDS             1583..1816
                     /locus_tag="VW852_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV0272871.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPIMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            1816..3150
                     /locus_tag="VW852_001784"
     CDS             1816..3150
                     /locus_tag="VW852_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV0272872.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIADESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            3160..3414
                     /locus_tag="VW852_001785"
     CDS             3160..3414
                     /locus_tag="VW852_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV0272873.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            3510..3905
                     /locus_tag="VW852_001786"
     CDS             3510..3905
                     /locus_tag="VW852_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV0272874.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            4449..4766
                     /locus_tag="VW852_001787"
     CDS             4449..4766
                     /locus_tag="VW852_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272875.1"
                     /translation="MERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFA
                     KILERLVAPELLDEKGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKD
                     GKN"
     gene            4753..5313
                     /locus_tag="VW852_001788"
     CDS             4753..5313
                     /locus_tag="VW852_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272876.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            5313..5891
                     /locus_tag="VW852_001789"
     CDS             5313..5891
                     /locus_tag="VW852_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0272877.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            5884..8535
                     /locus_tag="VW852_001790"
     CDS             5884..8535
                     /locus_tag="VW852_001790"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV0272878.1"
                     /translation="MSKELSSKYNPADVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAINPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            8605..9359
                     /locus_tag="VW852_001791"
                     /pseudo
     CDS             8605..9359
                     /locus_tag="VW852_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
     gene            9638..10441
                     /locus_tag="VW852_001792"
     CDS             9638..10441
                     /locus_tag="VW852_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV0272879.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQARGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADMVKKIQHFISGIWQIHPFREGNARTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT         3166 a         1964 c         2357 g         3050 t
ORIGIN      
        1 aaatcgattt gactgtcctg atcgatttgt cctgttcttg tttcatttca ctataaaaaa
       61 ggggttctgt attttttaat gttatctaat tagaaaatgc tttttttgta ggaaatataa
      121 tatgataagg tgcaaaaaag aaataaggag tttgtatatg gctgaacaag acttagctat
      181 gcaagtattg caacaagtgg tgaaactacc tgttgttaag gttgatcgtt cgaaattttt
      241 agtggataag ttttccaaag aattgggtcc acaggacatt cctaccttat tggaacaagg
      301 tccaacgtct ctcttatctc aagaaatctt agatcgagtg gctaatgcct gtattaggga
      361 taatgtttta ttagcgagtg ggacttctgt tttggcagga ttacctggag ggcttgctat
      421 ggcaattacc attccagctg atgtggctca attttatact ttctctctaa aattggctca
      481 agaattaggt tatatttatg gttatgagga tctttgggct tcacgcgagg agttgagtga
      541 agatgctcaa aataccctct tgctttatct aggcgtcatg ttgggggtga atggaaccgc
      601 tgctttgcta cgtgctggtg gtataacaat tgccaaacag gtaatgaaaa cagtgcctaa
      661 taaagcttta acaaagacgc tttggtaccc tattttgaaa aaagtcttaa aaatatttgg
      721 tgtgaatctt accaagggag ggctggccaa aggaataggg aaatttattc ctatcttggg
      781 tggtatcatt tcaggtggtt taacctttgc aactatgaaa ccgatgggtg aaagcttgca
      841 gaaagaattg tccaagctag ttaactatag tgaagttcaa tatcaagaag atgttgaaac
      901 aatccgaaaa gaggctgaaa tcatcgaagg agagtaatat gggactcata aaaactctag
      961 ctaaaattta tggtaattac tttttgaccg tgtaaggtgt aaaagtaatg aaaacgataa
     1021 agaaagatga taatgccgtt gtcggtctgg ggaaactttt tattgccgac aagttaatgg
     1081 atacggctcg gtggctcatt aagccagagg ataaaaaatg aaatttttct ggggacttct
     1141 tgccattatt tttatcaaac cgattattgg gattgtgaaa ttcttttgga tgatcatctc
     1201 ttttgcagtc caattgctgt tttacaagat agtgtttaag atattggatt ggctctttaa
     1261 acttatctag atggtaatcc aagtcgcaga gaactagcag gaactccact gctagttttt
     1321 tattctcttt ccatatggta taatataagc agtaaaatca ttttatactc ttcgaaaatc
     1381 tcttcaaacc acgtcagctt caccttgctg tatatatgtt actgacttcg tcagttctat
     1441 ccacaacctc aaaacggtgt tttgagctga cttcgtcagt tctatctaca acctcaaagc
     1501 actgttttga gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatt
     1561 agaacataca atggaggtcg tcatggacaa tatcatcgat gtgtcaattc ctgttgcaga
     1621 agtggtggac aagcatccag aagtcttgga aattctagtg gagttgggtt ttaaacccct
     1681 tgccaatccc ataatgcgca atacagttgg tcgtaaagta tcacttaaac agggttctaa
     1741 gctagcagga actcctatgg acaagattgt acgcacactg gaagcgaatg gctacgaagt
     1801 gattggatta gactaatgac agatgaacgg attcatatcc tacgggatat tttgttagaa
     1861 ttgcacaatg gcgcctctcc tgagtcggtt caggagcgtt ttgatgcgac ctttgcgggt
     1921 gtctcagcca ttgagatttc ccttatggag cacgagctga tgaactcaga ctcaggtgtc
     1981 acctttgaag atgttatgga actctgtgat gtccatgcca atctttttaa aaatgctatc
     2041 aaaggtgtcg aagttttaga taccgaacac cctgggcacc cagttcgtgt ctttaaggaa
     2101 gaaaatttgg ccctccgtgc agctttgatt cgcattcgta gattgttaga tacctatgag
     2161 tctatggaag acgaggaaat gcttgcggag atgcgcaagg gcttggtgcg tcagatggga
     2221 cttgtggggc aatttgacat ccattaccaa cgtaaggaag aactcttctt tcctatcatg
     2281 gagcgctatg gacacgattc acctcccaaa gttatgtggg gagtggatga tcagattagg
     2341 gaactctttc aaacagctct aacgacagcc aagtcactac cagaagtgtc aattagcagt
     2401 gtaaaggaag cttttgaagc ttttgcgaca gagtttgaaa gtatgatttt caaggaagag
     2461 tccatcctcc tcatgattct ccttgagtct tttactcagg atgactggct tcagattgcg
     2521 gatgagagcg atgcctatgg ctatgccatc atccgtccgt cagagaaatg ggtgccagaa
     2581 cgacagagct ttattgagga aaagattgca gaggagcctg tacagctaga tacggcagaa
     2641 ggtcaagttc aacaagtcat agatacgcca gaaggccatt ttaccattac ctttacccct
     2701 aaggaaaagg aagctgtgct ggaccgccat agtcaacagg cttttggtaa tggctatctt
     2761 tcagtcgagc aggccaatct catcctcaat catctcccta tggagattac ctttgtcaat
     2821 aaagaagata ttttccagta ttacaatgac aatacgccag ctgatgagat gattttcaaa
     2881 cggacgccgt cccaagtcgg gcgcaatgtc gaactctgtc atccgcctaa gtatttggac
     2941 aaggtcaaaa ctatcatgaa ggggcttcgt gaggggacca aggacaagta tgaaatgtgg
     3001 ttcaagtctg agtcgcgagg taagtttgtc cacatcacct atgctgcagt acacgatgaa
     3061 gacggagaat tccaaggagt gttggagtat gttcaggata tccagcccta ccgtgagatt
     3121 gatacggact attttcgtgg attagaataa ggagaaaaaa tgagttacga acaagaattt
     3181 atgaaggaat ttgaagcttg ggtcaatacc caaatcatga ttaacgacat ggcgcacaag
     3241 gaaagtcaaa aagtttacga agaagaccag gacgagcgtg ccaaagatgc catgattcgc
     3301 tacgagagtc gcttggatgc ttatcagttc ttgcttggta agtttgaaaa cttcaaagca
     3361 ggcaagggat tccatgattt gccagaaggc ttgtttggtg agcgaaatta ttaaacgaga
     3421 aagattcttg atttttcact aaaatcttga tagaatgttt atgttaaatc cttgtcagag
     3481 cagggatttt ttattgaaag gattttatca tgtcaaagaa actcaatcgt aaaaaacaat
     3541 tacgaaatgg cctccgtcgc gcaggtgcct tttcaagtac tgtgactaag gttgtagatg
     3601 agacaaaaaa agtcgtgaag cgtgcagaac agtcagcaag cgcagctggt aaggctgttt
     3661 ctaaaaaagt tgaacaagca gtagaagcta ccaaagagca agctcaaaaa gtagctaatt
     3721 ctgtagaaga ttttgcagca aacttgggtg gacttccact tgatcgtgcc aaaactttct
     3781 atgatgaagg aatcaagtct gcttcagatt tcaaaaactg gactgaaaaa gaactccttg
     3841 ccttgaaagg aatcggccca gctaccatca agaaattgaa agaaaatggc atcaagttca
     3901 agtaattttt cttgagcctt gcatttccga gaaaaacttg ctacaataga gccattagag
     3961 gtgttttgaa tcccacattt tacagaaagt ggcggcgctg agaagtccac aaatgtgtca
     4021 aaactggttg ctaatgactg aaaaaatgaa atatttctgt ctttttattt taaagactaa
     4081 agatgcgggc ttggcccgaa attgggtggt accgcggata aacacattcg tccctgtcat
     4141 gtagatggca gggcttttct tttgtgtctt agtcaaagga ggttgttata aaacaatcat
     4201 ttaaaactaa taaactatat tatggttttc ctgtttttat tttaggatat caagattaga
     4261 attttggcta taacatcacg acctgtagtt cctcttatag tctaggagat tgggttgtga
     4321 ttggagtcgt tgcgagagag aatgccgcag agcagatcaa acagtatcaa aaatttactg
     4381 tgaatatttc tgatgaaact tctatgcttg cgatggagta ggctggtttt atcagtcatc
     4441 aggagaaatt ggaacgtttg ggagtgcatt atgaaatttc tgaacgaact cagattccta
     4501 ttttagacgc ctgtccactt gttttagatt gtcgggtaga taggattgtt gaggaagacg
     4561 gtatttgcca catctttgcc aagattcttg agcgacttgt tgccccagaa ctcctggatg
     4621 aaaagggaca ttttaaaaat caactgtttg ccccaaccta tttcatggga gatggatatc
     4681 agcgcgttta tcgctatctg gacaagcgtg tagatatgaa gggcagtttc atcaaaaaag
     4741 cgaggaagaa ggatggcaag aactgagctg ccagataaaa tcgaaacaga acgtctcgtt
     4801 ttacgagtcc gtactgtggc ggatgctgag gatatctttg actatgctag tttgccagag
     4861 gtcgcctatc cagcaggttt tcctccagtc aagaccttgg aagatgagat ttattatctg
     4921 gagcacattc ttccggagcg taatcaaaag gaaaatctcc cagcaggcta cgggattgtc
     4981 gtcaaaggaa cggataagat cgttggctct atctatttca accatcgcca tgaagatgat
     5041 gtgatggaaa tcggctatac cttacactca gactattggg ggcgaggtta tgtgccagaa
     5101 gctgcgcgtg ccttgattga cttagccttt aaagatttgg gtcttcacaa gattgaacta
     5161 acttgctttg gatataacct tcaaagtcaa cgagtcgcgg aaaagcttgg ctttaccctc
     5221 gaagctcgca taagagaccg taaagatgct caaggcaact gctgtgatga tttgagatat
     5281 gccttgctga agagtgagtg ggaggtgatt tgatgcatct tttcatcatt ggtgctccag
     5341 catcaggaaa aatgaccatt ggtcaagaat tatctcgact gacggatgct accctatttt
     5401 ataaccatca agccattgat tttgcactag aaatctatca ggactataca gaggaaatgt
     5461 gggaatttgt tcgtggaatc actttttcat tccttggagc aagtgcaaga aatcagcgat
     5521 cagtaatttt aacagatgta attgattttt caaatcagta tcagctgatg tatttaaagc
     5581 aaattcagaa tttgttggat gattatcatc aagagattct ttttgttgag ttggaaacaa
     5641 gtcttgaaga gtgcatacgt cgaaatcgaa cggagaatcg attaaagcac aaacccttaa
     5701 aacgacatat tgaggtatct gaaagagaaa ttttagagac cgctgaaaca cttcaattaa
     5761 attcccaata tcaaccgaat gagttgcacc actactttaa aataaataat acgaatttgt
     5821 ctgcagaaga agctgctaag caaattcaaa ataaaataaa caaaatagag aaaggacaca
     5881 cacatgtcta aagaactttc atctaaatac aatccagccg atgttgaggc tggtcgttac
     5941 caaaaatggc ttgatgcgga tgttttcaag ccttcaggcg atcaaaaggc taagccttat
     6001 tcaatcgtga ttccaccacc aaacgtaact gggaaacttc accttggtca cgcttgggat
     6061 acgactttgc aggatatcat catccgtcaa aaacgcatgc agggctttga taccctttgg
     6121 cttccaggta tggaccacgc tggtattgcg actcaggcta aggttgagga gcgcttacgt
     6181 ggtgagggta ttacgcgtta tgacctaggt cgtgagtctt tcttgacgaa ggtctgggaa
     6241 tggaaagacg aatatgccac taccatcaag gaacaatggg gcaagatggg gctttctgta
     6301 gactattctc gtgagcgttt cactcttgac gaaggtttgt caaaagctgt tcgtaaggtc
     6361 tttgtggacc tttacaagaa aggctggatc tatcgtggtg agtttatcat caactgggac
     6421 ccagcagctc gcacagccct ttctgatatt gaggtgattc acaaggatgt agaaggtgcc
     6481 ttctaccaca tgaattacat gctggaagat ggttcacgcg cccttgaagt tgctacaact
     6541 cgtcctgaga ctatgtttgg ggacgttgcg gttgcgatta atccagaaga cccgcgctac
     6601 aaggacttga ttggtaaaaa cgtcatcctt ccaatcgcta ataaactcat cccaatcgtt
     6661 ggagatgagc acgcagatcc tgagtttggt actggtgtcg tgaaaatcac acctgcccac
     6721 gatccaaatg acttcttggt tggccaacgt cataacttgc cacaagtcaa cgtcatgaac
     6781 gacgacggaa ctatgaatga gcttgccttt gaattttcag gcatggatcg ttttgaagct
     6841 cgtaaggcag tcgttgctaa gttggaagaa atcggtgccc ttgtcaaaat cgaaaaacgt
     6901 gtccattcag tcggtcactc agaacggaca ggtgtcgtag ttgagccacg cttgtctact
     6961 caatggttcg tcaagatgga ccaattggct aagaacgcca ttgccaacca agacacagag
     7021 gacaaggtcg aattctaccc acctcgtttc aacgatacct tccttcaatg gatggaaaat
     7081 gtccacgact gggttatctc tcgtcagctt tggtggggtc accaaatccc tgcctggtac
     7141 aatgctgatg gtgaaatgta tgtcggcgaa gaagctccag aaggtgacgg atggactcag
     7201 gacgaagacg tcttggatac ttggttcagt tctgccctct ggccattttc aaccatgggc
     7261 tggcctgaag tcgactcaga agactttaaa cgttatttcc caacttcaac cttggtaaca
     7321 ggttacgaca tcatcttctt ctgggtgtct cgtatgatct tccagtcatt ggaattcaca
     7381 ggccgtcaac cattccaaaa cgtccttatc cacggtctca ttcgtgacga gcaaggacgc
     7441 aagatgtcta agtctctcgg taacgggatt gacccaatgg atgttatcga gaaatacggt
     7501 gccgatgccc ttcgatggtt cctttcaaac ggttctgcgc caggacaaga cgtgcgtttc
     7561 tcttatgaga aaatggatgc ttcatggaac tttattaaca agatttggaa catttctcgc
     7621 tatatcctca tgaacaatgg aggtttgacg ctggatgtgg cgcatgacaa tgtcacaaaa
     7681 gttgcaacag gtgaggctgg taatgtgacg gaccgctgga ttctccacaa tctcaacgaa
     7741 accattgcaa aagttactga aaactttgat aagttcgagt ttggtgtggc tggtcatatc
     7801 ctttacaact tcatttggga agaatttgcc aactggtatg ttgagttgac caaggaagtc
     7861 ctttatagcg ataacgaaga agagaaagtc atcacacgtt ctgttctact atacactttg
     7921 gacaagatcc ttcgtctcct tcacccaatc atgccattcg tgacagagga aatctttgga
     7981 caaatctcag aaggttctat cgtgacagct gcatacccaa ctgttaatct agcctttgaa
     8041 gaccttgcgg ctcacactgg tgtggaaagt ctcaaagact tgattcgtgc tgttcggaat
     8101 gcgcgtgcgg aagtaaatgt agcaccaagc aagcctatca ccatccttgt taagacaagc
     8161 gatagcgact tggaagcctt ctttaacagc aatgtcaact atatcaaacg ctttacaaat
     8221 ccagaacact tggaaatcgc atcaaccatc cctgcacctg aactggctat gtcaagcgtt
     8281 attacaggag ccgaaatctt cttgccactg gccgatctcc tcaatgttga agaagaacta
     8341 gctcgtctcg acaaagaact cgctaaatgg caaaaagaac tggacatggt tggtaagaaa
     8401 ctctctaacg aacgcttcgt cgccaacgct aaaccagaag ttgtccaaaa agaaaaagac
     8461 aaacaagccg actaccaagc caagtatgat gtgaccgtag cacgtattga tgagatgaag
     8521 aagttggtga aataaacaca gaaacacggt gatgagccgt gttttttggt ataatgaaat
     8581 caatatctat tgatggagaa agtgatgtcc aaacaccctc attatgaatt gttaaattta
     8641 attggctacg gtcttgccaa gtttgacaag ctttttataa aagaatttca atgctctagc
     8701 aagtcggagt tttatcgcta tgtggtttct ctgggaattg ctgaaacaac tggagttgta
     8761 aaaaatagaa tggatttatt tgatccttat tttgacaata atcgaaaagg ttggtggcag
     8821 aaagctgaag tttatcgttt tcgtaaagat ttaattgata tgatgtttgg aaatgaagat
     8881 gttcatagtt atgctgaata gttaaaatgt tacttgccag tgaagggaag aaaacaggca
     8941 taaccatcgt tgaaaaacca atagttcgaa ctaaattcaa acgtctacag gaaacgggaa
     9001 tggaggctga gaattatttt atccttcatt ttgataaaga agaaaaattc caaggtggac
     9061 agttaaccga tgcccgtctt tatggtgatg gatatgactt tcaggtagat gtccaagagt
     9121 attcttacct tgccgaagta aaaggtatcc gaaaatctaa ggggcgtgtg cgtttaactg
     9181 caaaagaatt tgaaaaagtc aaggagtttc aatcggattt tattttatcc ttggtcacaa
     9241 atctagatga tattccaaag ttagtgttaa tcgataatcc tttaaaacat tttgagttta
     9301 aaaagaatat tatcaaaaat gaaatcattg aatatagaag cgtagaggat ttgtattagt
     9361 tcccccttaa cttttccagt tgaaggaacg agaagtcttg ataggtcaac gacttaatga
     9421 actggaaatc aacggtattc gtttgactaa gttttaagat ggcgaaatcg gtattgaatt
     9481 tatcagtagt gctgattata actcttttat tatttacatg aagtatatat atatatatca
     9541 tagaacctat tgtgaggact ttttatagtt taagccagtt tccaaccaat ttcgtgattt
     9601 actggtaatg aagaaaaaca attgaaagga tatttctatg caaccaactt acaacattga
     9661 caatccaaac ttgtcttatg aagctaagcg tgatttatgg cggataggtt ttggtctgca
     9721 gaaagttgac aatctagtgc catcagctta tatggaatct ttggctgaga aacaggctcg
     9781 gggtgaactg acttatgagc aggtttatga ggatgcaacg gcttatcacc atactattga
     9841 tgcaagtacg gaggaggcag acttggtttc tctacgcatt gtagaactat tgtctcgaag
     9901 aggctttagc ttcagtcctg cgaccttact tgctattcat aaggagttgt ttcaagatat
     9961 atttgaaccc tcgattccgg taggtcaatt tcgtcagact aatatcacaa agaatgaacc
    10021 tgttttgaat ggtgaaagtg ttgtgtactc tgattactcc atgattcaaa tgacattgga
    10081 ttatgatttt aatcaggaaa aacaagttgc atatgcgaca ctaacccagg cggatatggt
    10141 taaaaaaatc cagcatttta tttcaggaat ctggcagatt catccatttc gcgaaggaaa
    10201 cgctcggaca gtaacggtct ttttgattca gtatcttcgt gagtttggtt ttgatattga
    10261 taatatacca tttcagcaac attccaagta ttttcgtgat gccttggtat tagataatgc
    10321 aaagatttta cagcgacgtc ctgagttttt aacagctttt tttgaaaatc tcttgctcgg
    10381 tggtcaaaat gatttgtctt cagaaaaaat gtatctagat ttagacctcg atttttcata
    10441 atccaaatac tgagtaaaca ttgaatttta ggaaaaaatg aagtaaacat tcccacaata
    10501 aaacgcatag tatcaagtgt taaaatgtac tgccccc
//
LOCUS       DAVKWS010000063        10271 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKWS010000063 DAVKWS010000000
VERSION     DAVKWS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.064"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VW852_001793"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VW852_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV0272880.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1318..2022
                     /locus_tag="VW852_001794"
     CDS             1318..2022
                     /locus_tag="VW852_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272881.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2015..3364
                     /gene="vicK"
                     /locus_tag="VW852_001795"
     CDS             2015..3364
                     /gene="vicK"
                     /locus_tag="VW852_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV0272882.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     LLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3366..4175
                     /locus_tag="VW852_001796"
     CDS             3366..4175
                     /locus_tag="VW852_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0272883.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4266..4508
                     /locus_tag="VW852_001797"
     CDS             4266..4508
                     /locus_tag="VW852_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV0272884.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4510..4764
                     /locus_tag="VW852_001798"
     CDS             4510..4764
                     /locus_tag="VW852_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV0272885.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVRIVDDELVVATLEVGHRRDIY"
     gene            4811..5554
                     /locus_tag="VW852_001799"
     CDS             4811..5554
                     /locus_tag="VW852_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV0272886.1"
                     /translation="MNKKWLFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLTKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLWTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGVLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILIEKQSEKIEELH"
     gene            complement(5843..6829)
                     /locus_tag="VW852_001800"
     CDS             complement(5843..6829)
                     /locus_tag="VW852_001800"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV0272887.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7023..9491
                     /gene="gyrA"
                     /locus_tag="VW852_001801"
     CDS             7023..9491
                     /gene="gyrA"
                     /locus_tag="VW852_001801"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV0272888.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVIIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9491..10234
                     /gene="srtA"
                     /locus_tag="VW852_001802"
     CDS             9491..10234
                     /gene="srtA"
                     /locus_tag="VW852_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV0272889.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT         3172 a         1750 c         2291 g         3058 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgt ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccaggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagcctg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tctggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggtcccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtgcagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgc tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact atttttttgg tataatagtg gagaaaaagg tgaacatatg
     1321 aaaaaaatac taattgtaga tgatgagaaa ccaatctcgg atattatcaa gtttaatatg
     1381 accaaggaag gttacgaagt tgtaactgct tttaatggtc gtgaagcgct agagcaattt
     1441 gaagcagagc aaccagatat tattattctg gatttgatgc ttccagaaat tgatggttta
     1501 gaagttgcta agaccattcg taagacaagc agtgtgccca ttcttatgct ttcagccaaa
     1561 gatagtgaat ttgataaggt tatcggtttg gaacttgggg cagatgacta tgtaacgaaa
     1621 cccttctcca atcgtgagtt gcaggcgcgt gttaaagctc ttctgcgtcg ttctcaacct
     1681 atgccagtag atggtcagga agcagatagt aaacctcaac ctatccaaat tggggattta
     1741 gaaattgttc cagacgccta cgtggctaaa aaatatggcg aagaactaga cttaacccat
     1801 cgtgaatttg agcttttgta tcatttagca tcgcatacag gtcaagtcat cacgcgcgaa
     1861 cacttgcttg agactgtctg gggttatgac tattttggtg atgtccgtac agttgatgtg
     1921 actgtacgac gtctgcgtga gaagattgaa gatacgccca gccgaccaga gtatatcttg
     1981 acgcgccgtg gtgtagggta ttacatgaga aataatgctt gatttactga aacaaaccat
     2041 ttttaccaga gattttatct ttatcctgat tttgttaggt ttcatccttg ttgtgaccct
     2101 cttattactg gaaaatagac gtgataatat tcagttgaag caaatcaatc aaaaggttaa
     2161 agatttgatt gcaggagatt attccaaggt tcttgatatg caaggtgggt ctgaaatcac
     2221 caatattacc aataatttga atgacttgtc ggaggttatt cgtctcactc aggaaaatct
     2281 agaacaagag agtaagaggc taaatagtat tctgttttat atgacagatg gggttcttgc
     2341 gaccaaccgt cggggtcaga ttatcatgat taatgataca gccaagaagc aactggggtt
     2401 ggttaaggaa gatgttctga atagaagcat tttggaattg ctcaagatag aagaaaacta
     2461 tgaattgcgt gatttgatta cccaaagtcc agaattgttg ctagattccc aagatatcaa
     2521 tggcgaatat ttgaaccttc gagttcgctt tgccttgata cgtcgagagt ctggctttat
     2581 ttcaggtttg gtggctgttt tgcatgatac gacggagcag gagaaggaag aacgagaacg
     2641 aagactcttt gtttccaatg ttagccatga gttacggact cctctgacta gcgtaaaatc
     2701 ctatcttgaa gccttggatg agggggcttt gtgtgaaact gtagcaccag actttatcaa
     2761 ggtttctctt gatgagacca accgtatgat gcgcatggtg acggatctcc tccatctttc
     2821 acgtattgat aatgctacca gtctcctaga tgtggaactg attaacttca ctgcttttat
     2881 tacctttatc ctcaatcgtt ttgacaagat gaaaggacag gaaaaggaga aaaaatatga
     2941 gttggtgaga gattatccca tcaattctat ctggatggaa attgatacag ataagatgac
     3001 gcaggttgtc gacaatattt taaataatgc tattaagtat tcgccagatg ggggtaaaat
     3061 cactgtcaga atgaagacaa ctgaagacca gatgatttta tccatttctg accacggttt
     3121 ggggattcct aagcaggatt taccacgtat ctttgaccgt ttctatcgtg tggatcgtgc
     3181 tagaagtcgt gcacaaggtg gtacaggtct aggactgtct atcgctaaag aaattatcaa
     3241 acaacataag ggctttattt gggccaagag tgaatacggc aagggttcaa cctttaccat
     3301 tgtgctccct tatgataagg atgcagtgaa agaagaagta tgggaggatg aagtagaaga
     3361 ctagaatgag tgaaataggc tttaaataca gtattttagc gtcgggttcc agtggaaatt
     3421 ctttttatct ggaaacctca aaaaagaagc ttttagtaga tgcaggcttg tctggcaaga
     3481 aaattaccag tctgctagct gaaattaacc gtaagccaga agacctggat gccatcttga
     3541 ttacccatga gcattcagat catatccatg gagtaggcgt tttggctcgc aagtatggta
     3601 tggatcttta tgccaatgaa aagacctggc aagctatgga aaatagtaaa tatcttggca
     3661 aggtggattc ttcgcaaaag catatttttg aaatgggtaa aaccaaaacc tttggagata
     3721 tcgacatcga gagttttggt gtaagccatg atgcagtcgc accgcagttc tatcgcttta
     3781 tgaaggatga taagagtttt gtccttttga cagatacagg ttatgtcagt gaccgtatgg
     3841 cgggcattgt cgaaaatgcg gatggctatc ttatcgaggc caaccatgat gtagagattt
     3901 tgcgatcagg ttcttacgct tggcgactca aacaacgaat cctatctgac cttggtcacc
     3961 tttctaacga ggacggtgct gaagctatga ttcggacgct aggaaatcgt actaagaaga
     4021 tttaccttgg gcatttatct aaggaaaaca atatcaagga actggctcat atgaccatgg
     4081 tcaatcagct ggctcaagct gatctgggag tcggagtaga ctttaaggtt tatgatacct
     4141 caccagatac cgcaacacca ttgacagaga tatagaaaga acgctgagaa ggtgttcttt
     4201 ttatattgac tggacaccta aaaagtaata caatggtgtt accattaaaa aagggagcac
     4261 aaaagatgac tactattaca ttaaaagttt ctgaagctga taaaacattt atgaaagcaa
     4321 tggctaagtt tgaaggagtt tccctttcgg aacttattcg caccaaaact cttgaagccc
     4381 tagaagatga atacgatgct cgtgtggcag atttagccta tcaagagtat ttagaagact
     4441 tggaaaaagg agttgaaccc attacttggg aagaaatgat gcatgattta ggcttgaagg
     4501 atgaataatt tgtataaatt agttccaaca agacgtttta tcaagcaatt gaaaaaattg
     4561 gaccgttata cgcagaagct aattacaaac tatttacaaa ccaatgtttt ggaagaccca
     4621 agacgacacg gaaaggcttt ggttggtaat cgcgttggtc aatggcgcta tagaattggt
     4681 aattatcgag ttatcgtacg aattgtagat gatgaattag ttgttgctac tctagaagtt
     4741 ggtcatcgga gagatattta ttgaattaaa gctgagtaat aacagtatag aaagtaagga
     4801 tagtaaaaat ttgaataaaa aatggttatt tgcagattat tatgatacaa caattatttt
     4861 acttgcttta atctctgtta ttctggtatt acttggattt gctgagatga ttgatttgga
     4921 caatccgcct tatagtatta ttgatttagt aatctggggt gtttttgtaa ttgattatag
     4981 ttggcgcttt tttacgacta aaagaaaatg gcgctttatt cttgaaaata tttttgattt
     5041 attggctatt ctccctttaa atgctatttt cacagttttt cgattagggc gtatctttcg
     5101 tttaactaaa ctaacaaaat tactgaaact gactcgttta cttagaataa ttggacttac
     5161 aggtaaatta gaaagaaaaa ttagtagatt cttatggaca aatggcctga tttatatctt
     5221 gtatgttaat atctttattg ttctagtagg gagttcgatt ttatccgttg ttgaagaaaa
     5281 atccttctca gatagtcttt ggtgggctct tgtgacagta actactgttg gttatggtga
     5341 tattgttcca gtttcccttt ttgggaaatg gctagcagtt ttactgatgc ttgtcggtat
     5401 tggaacaata ggggtgctaa caagtgcttt gacgaacttt tttgtaaagg ataatccaga
     5461 tgaacagata aaacttgata aactcaaaga cgaattatcg agccaaagaa tattaataga
     5521 gaaacaatct gaaaaaatcg aagaactaca ttgaatgata aaagacttag ttgaaaaaat
     5581 atcataaagg tgagacaaaa caaaacttga ttcgaggaga aatcaagtaa gtcttgacac
     5641 aatagaacgc atattatcag gtataaaaac ttgatactat gcgttttata gagaaataaa
     5701 ataaaatctg aacaaatcaa ttaggaattt aaagcatttt ataacaacgt tttagagtga
     5761 tggggtgcta tttcaacttc aacctactat aatacagaaa aaaacaactc cctgatgatt
     5821 caaggagttg tctatagtta aattagtttt tagaagcttc ttggaattct ggatttttcc
     5881 atgcttcgtc aataatagct tgcaattctt tagcagatgc ttgcattttt tgagtttctg
     5941 catcgttcaa tggaatgttt actggacgaa cgataccatg tgcaccaaca acagctggtt
     6001 gaccgataaa gacattctca actccgtatt gaccttcttg gaatactgaa agtggaagta
     6061 ctgcgttttc atcgtcaagg attgctttag tgatacgagc aagggctact gcgataccgt
     6121 agtatgttgc accttttttg ttgatgattg tgtaggctgc atcacgaaca ccttcgaaca
     6181 attcaatcaa ttcagcttct tgaacatttt gagtgtcttt aaggaattct tcaaggttta
     6241 caccagcgat gttagcgtgt gaccaaacag cgaactcaga gtcaccgtgt tcacccatga
     6301 tgtaggcgtg cactgaacga gcatccacat ccaatttttc agcaagtgct tgacggaaac
     6361 gagctgagtc aagtgaagta cctgaaccga taacgcgttc tttagggaaa ccagagaatt
     6421 tccaagttga gtaagtcaaa acgtcaactg ggttagcagc aacaaggaag atacctttga
     6481 aaccagattc aacaacttga gttacgattg atttgttgat agcaaggttt ttacctacaa
     6541 ggtcaagacg agtttcacct ggtttttgag gtgcacctgc agtgatcaca acaaggtcag
     6601 cgtctgcaca gtcagagtat tgagctgcat agattttttt aggtgaagtg aaggcaaggg
     6661 cgtgactaag gtcaagcgca tcaccaacag ctttttcatg caattgtgga atttcgataa
     6721 ttccaagctc ttgtgcaatt ccttggttaa caagtgcaaa agcgtaagat gaacctacag
     6781 caccatcacc gacaaggata acttttttgt gttgtttagt tgaagtcatt gttttaaaca
     6841 tctccttaat tttattaggg gattttccct agacaacttc attctatcac ttttaaaaaa
     6901 ctttgtcacg aatatgcctt atagttctcg atgtaaacgt tttagtggtt tagaggctga
     6961 aatagatggg aatttatggt ataatgttgt tacttactaa ttgtgaaatg aggcatttat
     7021 taatgcagga taaaaattta gtgaatgtca atctgacaaa ggagatgaag gcaagtttta
     7081 tcgactacgc catgagtgtt atcgtagcgc gagctcttcc tgatgttcga gatggcttaa
     7141 aacctgttca ccgtcgcatt ctctacggaa tgaatgaatt gggtgtgacc ccagacaaac
     7201 cccataaaaa atctgctcgt attacagggg atgtcatggg taaatatcac ccacacgggg
     7261 attcctctat ttatgaagcc atggtccgta tggctcaatg gtggagctac cgttacatgc
     7321 ttgtagatgg tcatgggaat tttggttcca tggatggaga tagtgctgcc gctcaacgtt
     7381 ataccgaggc acgtatgagc aagattgctc tggaaatgct tcgtgatatc aacaaaaata
     7441 cagttgattt cgttgataac tatgatgcca atgaacggga acccttggtc ttgccagcgc
     7501 gttttccaaa ccttttggtt aatggagcaa ctggtatcgc ggttgggatg gcaaccaata
     7561 ttccacctca taatctgggt gaaaccattg atgcagtgaa gttggtcatg gataatcctg
     7621 aagtgactac caaggacttg atggaagtct tgcctggacc agattttcca actggtgctc
     7681 ttgtcatggg gaaatcaggt atccataagg cttatgaaac aggtaaaggt tcgattgtcc
     7741 tacgttctcg tacagagatt gaaacgacta agactggtcg tgagcgtatc gttgtaacag
     7801 aatttcctta tatggtcaat aaaaccaagg tgcatgagca tattgttcgc ttggttcagg
     7861 aaaaacgcat tgagggtatc acggcagtac gtgatgagtc aaaccgtgaa ggtgttcgat
     7921 ttgttattga agtcaagcgc gacgcctcag ccaatgttat tctcaataac ctcttcaaaa
     7981 tgacccaaat gcaaaccaat tttggtttca atatgctcgc tatccaaaat ggtataccga
     8041 aaattttgtc tcttcgtcag attttggatg cttatattga gcaccaaaaa gaagtggttg
     8101 ttcgtcgtac acgttttgat aaggaaaaag cggaagcgcg cgctcatatc ttagaaggtc
     8161 tcttgattgc gctagaccat atcgacgaag tgattcgtat catccgtgct agtgaaacgg
     8221 atgcggaagc tcaagctgag ttgatgagca agtttaagct ttctgaacgt caaagtcaag
     8281 ctatccttga tatgcgtctt cgtcgtttaa caggtttgga acgcgataag attcaatctg
     8341 agtatgatga cctcttggct ctgattgcgg atttagcaga tattcttgct aagcctgaac
     8401 gtgtttctca aattatcaaa gacgaattgg atgaagttaa acgtaaattt tctgataaac
     8461 gccgtacaga gttgatggtt ggacaggtct tgagtctcga ggatgaggac ttgattgaag
     8521 aatcggatgt cttgattacc ctttctaaca gaggctacat taagcgtttg gatcaggacg
     8581 agttcactgc tcaaaaacgt gggggtcgtg gtgtccaagg aacgggagtg aaagatgatg
     8641 actttgttcg tgagttagtg tcaactagca cccatgatca tctgctcttc ttcacaaaca
     8701 agggacgtgt ctatcgtctt aaaggttatg aaattcctga gtatggtcgg actgccaaag
     8761 ggctaccagt agtcaatctc ttgaaattgg atgaagacga aagtattcag acggttatca
     8821 atgttgagtc tgatcgcagt gatgatgctt atctcttctt tacaacccgt cacggtattg
     8881 tgaagagaac tagtgttaag gagtttgcca atattcgtca aaatggtctc aaagcgctga
     8941 atttaaagga tgaagatgag ttaatcaatg tcttgttggc agaaggagat atggatatta
     9001 tcattggtac caagtttggt tatgcagttc gctttaatca atcagccgtt cgtggtatga
     9061 gccgtatcgc cactggtgtg aaaggtgtta accttcgtga aggagacaca gttgttggtg
     9121 ccagcttgat tactgatcaa gatgaggttc ttattatcac agaaaaagga tatggtaagc
     9181 gtacagtcgc tactgaatac ccaacaaaag gtcgtggtgg taagggaatg cagacagcta
     9241 aaattaccga aaaaaatggc ttgctggccg gtcttatgac tgttcaaggg gatgaggatt
     9301 tgatgattat cactgataca ggtgtcataa ttcgaaccaa tcttgccaat atttcacaaa
     9361 caggacgtgc aactatggga gttaaagtaa tgcgcctgga tcaagatgct cagatagtga
     9421 ctttcacaac ggttgcggtg gcagaaaaag aagaagttgg gacagaaaac gaaacagaag
     9481 gtgaagcata atgtctcaaa aaaataataa aaagaaaaac aagcgaaaaa atctgctgac
     9541 aaatatccta gcaggatttc tgatattact gtcactggct ttgattttta atactcaaat
     9601 tcgaaatatt ttcatagtct ggaataccaa taagtatcaa gttagccagg tatcaaaaga
     9661 aaaattagaa gaaaatcagg atacagaagg caattttgac tttgattctg tcaaagctat
     9721 ctcttcggaa gctgttctaa cttctcaatg ggatgctcaa aaattaccag ttattggggg
     9781 aattgcaatt cctgaattgg aaatgaattt gccgattttt aaaggacttg ataatgttaa
     9841 tctcttctac ggagctggta caatgaaacg cgagcaagta atgggagaag gaaattatag
     9901 tctagctagt caccatatct ttggtgttga taatgctaat aaaatgttat tttctccttt
     9961 agataatgct aaaaatggca tgaagattta tctaaccgat aaaaataaag tttatactta
    10021 tgaaatacgt gaagtcaaac gtgtgacacc ggatcgtgtt gatgaagttg atgatagaga
    10081 tggggtcaat gaaatcacat tagtaacctg tgaagacctt gctgctacag aacgtattat
    10141 tgtcaaaggt gatttgaaag aaacaaaaga ttattcacaa acatctaatg aaatcctaac
    10201 agctttcaat caaccatata aacaatttta ttaatacaaa tcagtgaaat ccatgatttc
    10261 actgattttt t
//
LOCUS       DAVKWS010000064        10088 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKWS010000064 DAVKWS010000000
VERSION     DAVKWS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.065"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            524..907
                     /locus_tag="VW852_001803"
     CDS             524..907
                     /locus_tag="VW852_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV0272890.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            959..1648
                     /locus_tag="VW852_001804"
     CDS             959..1648
                     /locus_tag="VW852_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0272891.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1690..1938
                     /locus_tag="VW852_001805"
     CDS             1690..1938
                     /locus_tag="VW852_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272892.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKNDV"
     gene            1968..2579
                     /locus_tag="VW852_001806"
     CDS             1968..2579
                     /locus_tag="VW852_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0272893.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2740..3534
                     /locus_tag="VW852_001807"
     CDS             2740..3534
                     /locus_tag="VW852_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV0272894.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3531..4166
                     /gene="trmB"
                     /locus_tag="VW852_001808"
     CDS             3531..4166
                     /gene="trmB"
                     /locus_tag="VW852_001808"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV0272895.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4292..4771
                     /gene="rimP"
                     /locus_tag="VW852_001809"
     CDS             4292..4771
                     /gene="rimP"
                     /locus_tag="VW852_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV0272896.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4815..5951
                     /gene="nusA"
                     /locus_tag="VW852_001810"
     CDS             4815..5951
                     /gene="nusA"
                     /locus_tag="VW852_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV0272897.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5973..6266
                     /locus_tag="VW852_001811"
     CDS             5973..6266
                     /locus_tag="VW852_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV0272898.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6259..6558
                     /locus_tag="VW852_001812"
     CDS             6259..6558
                     /locus_tag="VW852_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV0272899.1"
                     /translation="MNKQKMSNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6575..9367
                     /gene="infB"
                     /locus_tag="VW852_001813"
     CDS             6575..9367
                     /gene="infB"
                     /locus_tag="VW852_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV0272900.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKEQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9618..9968
                     /gene="rbfA"
                     /locus_tag="VW852_001814"
     CDS             9618..9968
                     /gene="rbfA"
                     /locus_tag="VW852_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV0272901.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3068 a         1839 c         2299 g         2882 t
ORIGIN      
        1 ggtgctgcta cattatgtta aaaatcgctc ttgattgata agttggattt gttaggaaag
       61 gagtaaataa ttatggataa gaaaaagata gtttcgacta taatatgtat agtatttctt
      121 gtagtgtcag tagataattt ttttagagac ttaaccccgt tactgttcat tttaaatatt
      181 attggcctgt cttgcttttc agtgctaaca tacattaaca taaaagagat actcttaaat
      241 ataagtaaat gatgatttgg ttagtcctag aaccaatcaa cttatgttta tcggcttggc
      301 agattttatg tttgtcattt gtttgtatag aggtatttca gaaacagaat tctatcaaca
      361 actaatcgct tatataggtg tattctctgc ttgtttatca cgcttctgct catgtggggc
      421 ttgaagtatt ataaaaagta gctttctatc cttttctgac aggatatttg agtagaaggc
      481 tctattaaat tgttagtgaa ataaatgaat ggaggtattt aatatgaaat acagattatt
      541 ttttgttatt ttcttgagta gtgtgttgga tattctttta gggacatttt tacaaatctc
      601 tatcgtatct attgggtggc ttgttcttta cagtggtttg tttgaagcag gagttttcct
      661 tcttgctaat aaaggggtgg cggtaaaaat caaggaagta gatattcgaa atcgctttaa
      721 atttattttt ggaaaaacct tatggtttca aattcttttg ctcatctttt tgataatcaa
      781 actttatctt ggtttggatg cgaggttgat tttattctat ggacatattt tcattgtctt
      841 taatgcctta atgtatctat tatctagtag tcaggttagc cttaaaaaaa acaaactgtc
      901 ttcttaatct tacagcagta gagcctgaca aggtgaggtc atagtaggga ggatttctat
      961 gaaaaagtat caacttctat tcaaaataag tgcagtcttc tcttacttat ttttcgtatt
     1021 tggtctttct cagctgacgc ttatcgtcca aaactattgg caattttctt ctcagatagg
     1081 caatttattc tggattcaaa atatcttgag tttacttttt attggagtca tgattgtggt
     1141 tcttgttaag acaggccatg gttatctctt ccgcattcca agaaaaaaat ggctttggta
     1201 ttcgattttg acagtattag tggtagtgtt acagatctct tttaacgttc agacagctaa
     1261 acatgttcag tcaactgcgg aaggttgggc tgtattgatt ggttatagtg ggactaactt
     1321 tgcagagcta ggtatttata tagccctgtt ctttctggtt ccactgatgg aagaattgat
     1381 ttatagagga ttactgcaac atgctttctt taagcattcg cgatttggcc ttgatttgct
     1441 tcttccttcc attttgtttg ctcttcctca ttttttgagt ctgcctagtc tgttagatat
     1501 cttcgtcttt gcaacatttg gaatcatctt tgccggtttg acccgctata ccaagagtat
     1561 ttatccatcc tatgcggtgc atgtgatcaa taatattgta gcgaccttcc cgtttttgct
     1621 cacttttcta catagggtct tggggtaaaa aaacaaaagg cttgcttttc agccatagag
     1681 gaggtcatca tgtataaaca cttatttttc ctagattcca aaactttaga tcggttgaca
     1741 ccctatattc tagtcttggc ttctgacacc attgctttta atgtttttgt gctaaccttt
     1801 gtatctgcgg tggtttttaa tttcctaaat tccatgctag ctttaatggc tatattcata
     1861 ggggctggct atgtggtcgg attttggttg ctaaaatggt ttgttttgga aaggctagaa
     1921 ctaaagaatg acgtgtagga agtctgtttg ttgaggagga taattttatg gagttttttg
     1981 ataaatttca tgccttgtgt tttggatttt tagtactaat aattgtcatt acagttcctt
     2041 atacgattaa ccatggggat ttttttcaaa atgaatctgc attgattatt gtaagtcttc
     2101 ttgtaacctc gctgagtgtt gcttatgcta gaaagtttga aatgatttct tttgggatgt
     2161 taagcaagaa acaacttttg cttttcattg caatctttct tctaagtgta cttgagacgc
     2221 tggtttatat tcatttcttc gctgtttctt ctggctcagg ggtccaacac ttggcggaag
     2281 tcagcagagg aatttccctg tctttgattt taactacctc agtttttggc cccatccagg
     2341 aggaactcat tttcagagga cttcttcaag gtgcggtttt tgacaattct tggttagggc
     2401 ttgtgctaac ttcctctctc ttttctttca tgcatggacc ttctaatgtc ccttcgttta
     2461 ttttttatct acttgggggt ttgttgctgg gctttgctta taaaaagagt caaaacctat
     2521 gggtttctac tctagtccat atgctttaca acagttggcc actcttatat tatttataaa
     2581 aattatgaaa aggtaagcag aagacggtgc ttaccttttt caagctataa tgatacccaa
     2641 gccaatccag aggcttttct ttgagaatcg ggttagagtg gttgacgaat aggccaaaaa
     2701 ctagtagaat agtaaggaaa ctttatacgg aggaaagaaa tggatttggg tgataatgag
     2761 ctaacactga ctcccatacc tgggaaaagt ggcaaggctt atatgggtag ctatcctgat
     2821 gggaagcgca tctttgtaaa aatgaacacc tctccaatcc tacctggtct agctagagaa
     2881 caaattgctc cacaattatt atggagtcgt cgtttggcag atgggcgtga tatgtgtgct
     2941 caagaatggt tgacaggcaa gatattgacc ccctatgata tgaatcgtaa gcaaatcgtc
     3001 aatattttaa cccgtcttca tcgctcacgt ccgttgatga cacaattgag tcgtttgggc
     3061 tatgccatgg aaacacctgt agatttacta cagtcttggc aggaaacggc tccagatgct
     3121 ttgcgtaaaa atcattttat cagtgaagtg atggctgatt tacgtcagac tattccagga
     3181 tttagagagg accatgcgac cattgtccat ggagatgtac gacatagtaa ttggattgag
     3241 acagatagtg gcttgattta tttagtagat tgggattcgg ttcgcttgac cgatcgcatg
     3301 tttgatgtgg cccatatgct ctgccattat atttcagaac atcagtggaa ggaatggttg
     3361 acctactacg gttacaagta caatcaaacg gtattaagta aattgtattg gtatggtcaa
     3421 ttgtcttatt tgagtcagat ttccaagtat tatatgaacc aagatttaga aaatgtcaat
     3481 cgggagattc atggtttgcg tcatttccga gacaagtatg gaaagagaag atgagagtta
     3541 gaaatcgtaa aggggcaaca gaattactag aggcaaatcc ccagtatgtg gtcctcaatc
     3601 ccttggaagc caaggcaaaa tggcgggact tgtttggcaa tgataatccc attcatgtgg
     3661 aagttggaag tggaaagggt gcctttgttt caggtatggc caagcaaaac cctgacatca
     3721 actatatcgg gattgatatt caaaagtctg ttttgagcta cgctttggac aaggtgcttg
     3781 aagctggagt gtctaacatc aagctcttgt gggtagatgg ttctgacttg actgactact
     3841 ttgaagacgg tgagattgat cgcttgtatc tgaacttttc agatccatgg ccgaaaaaac
     3901 gccatgaaaa gcgtcgtttg acctacaaga cctttttgga taccttcaaa cgtatcttgc
     3961 ctgaaaatgg agaaattcat ttcaagacgg ataaccgtgg cttgtttgag tacagtttag
     4021 tgagcttttc tcaatatggc atgaaactca atggtgtctg gctagatttg catgccagtg
     4081 attttgaagg caatgtcatg acagaatacg agcaaaaatt ctcaaacaag gggcaagtta
     4141 tctaccgagt tgaggcagaa ttttaagaga tagcctaaaa ttaggctgta caagtgcttt
     4201 tgctttacat aagttggcaa acgtgctata ctgatagtaa gaatatgaaa agtgaggcgg
     4261 ggaaatatct tcgcctcttg cttatgagga ggtggacgca atcgcaacaa tcgtagaatt
     4321 agtcagagaa gttgtagaac ctgtcataga agctcctttt gaactcgtgg atatcgagta
     4381 tggaaagatt ggcagtgaca tgattctcag tatttttgta gataaacccg aaggaattac
     4441 cttgaacgac acggcagact tgacagaaat tatcagtcct gtcctagaca ccatcaagcc
     4501 agatcccttc ccagaacaat atttcctaga aattaccagt ccaggtttgg aacgtccttt
     4561 gaaaaccaag gatgccgtcg ctggagcggt tggaaaatac atccatgtcg ggctctacca
     4621 agccatcgat aagcaaaagg tctttgaagg aaccttgttg gccttcgaag aggacgagtt
     4681 gactatggaa tatatggaca agacgcgtaa gaaaaccgtc caaattccat acagtttagt
     4741 atcaaaagca cgtttagcag ttaaattata gaaaaagaaa ggatagcttt tgaggattca
     4801 aaagtgaaga aaacatgagt aaagaaatgc tagaggcctt ccgcattttg gaagaagaca
     4861 agggaatcaa aaaagaagat atcatcgacg cagtagtaga gtcgcttcgt tccgcttatc
     4921 gcagacgcta tggtcagtca gacagcgtag ctattgactt caacgaaaaa acaggtgact
     4981 ttacagttta tactgtccgt gaagttgttg atgaagtatt tgatagccgt ttggaaatca
     5041 gcttgaaaga tgctcttgcc attaattcag cttatgaact tggagacaaa atcaagtttg
     5101 aagaagcacc agctgagttt ggtcgtgtag cagcccaatc tgccaaacaa accatcatgg
     5161 aaaaaatgcg caagcaaaca cgtgccatca cttacaatac ttacaaagaa catgagcaag
     5221 aaatcatgtc tggtacagta gaacgctttg acaaccgctt tatctatgtc aaccttggta
     5281 gcatcgaagc ccaattgtca aaacaagacc aaattcctgg agaagttttt gcttctcatg
     5341 atcgtatcga agtttatgtt tacaaggttg aagacaaccc tcgtggtgtg aacgtctttg
     5401 ttagccgtag ccatccagaa atgatcaaac gtttaatgga gcaagaaatt ccagaagttt
     5461 atgatggaac tgttgaaatc atgagcgtgg ctcgtgaagc aggtgaccgt acgaaggttg
     5521 ctgttcgtag ccacaatcca aacgtggatg ctatcggtac aatcgttgga cgtggtggtg
     5581 ctaatatcaa gaagattact agcaaattcc acccagctcg ttacgatgct aaaaatgacc
     5641 gcatggtacc aatcgaagaa aatatcgatg ttatcgagtg ggtagcagat ccagctgaat
     5701 ttatctacaa tgccatcgct cctgctgagg ttgaccaagt tatctttgat gaaaacgaca
     5761 gcaaacgtgc cttggtggtt gttccagata acaagctttc tcttgccatt ggtcgtcgtg
     5821 gacaaaacgt gcgcttggcg gctcacttga ctggttaccg tatcgatatc aagtctgcta
     5881 gcgaatttga agccatggaa gacgctgctt cagtagagtt ggaagtagaa aacgatactg
     5941 tagaagaata aaagctgcta gaggagggaa agatgaaaac aagaaaaatc cctttgcgca
     6001 agtctgttgt gtctaacgaa gtgattgata agcgtgattt gctccgcatt gtcaagaaca
     6061 aggaaggaca agtctttatt gatcctacgg gcaaggccaa tggccgcggc gcttatatca
     6121 aactagacaa tgcagaagcc ctagaggcga aaaagaagaa ggtctttaac cgcagcttta
     6181 gcatggaagt ggaagaaagc ttttatgacg agttgatcgc ttatgtggat cacaaagtga
     6241 aaagaagaga gttgggactt gaataagcaa aagatgagta atctcttggg gcttgctcag
     6301 cgagcagggc gcatcatatc gggtgaagaa ttggtggtca aggccattca agacggcaag
     6361 gccaagttgg tctttctagc tcatgatgct ggacccaatc tgaccaagaa gattcaagat
     6421 aaaagtcatt attatcaagt agaaattgta accgtgtttt caacactgga attaagcata
     6481 gcagtcggga aatcgagaaa ggttttggct gtaacagatg ctggatttac aaagaaaatg
     6541 aggtctctta tggaatagaa gaggaggaca tgatttgtct aagaaaagat tgtacgaaat
     6601 cgcaaaagaa cttggaaaag aaagtaaaga agttgtagcg cgtgcaaaag agttgggctt
     6661 ggatgtgaaa agccactcat caagtgtgga agaagctgtc gctgcaaaaa ttgctgccag
     6721 ctttaagcct gcagctgctc cgaaagtaga agcaaaacct gcagcaccaa aagtaagtgc
     6781 agaaaagaaa gccgaaaaat ctgagccagc taaaccagct gtagctaagg aagaggcaaa
     6841 accggctgag ccagttgctc cgaaaacaga aaaagtagca gcgaaaccgc aaagccgtaa
     6901 tttcaaggct gagcgtgaag cccgtgctaa agagcaggca gaacgacgca agcaaaataa
     6961 gggcaataac cgtgaccaac aacaaaacgg aaaccgtcag aaaaacgacg gccgtaatgg
     7021 tggaaaacaa ggtcaaagca accgcgacaa tcgtcgcttt aatgaccaag ctaagaagga
     7081 gcaaggtcag caaaaacgta gaaatgagcg ccgtcagcaa gaggacaaac gttcaaatca
     7141 agtggctcca cgtattgact ttaaagcccg tgcagcagcc ctaaaagcag agcaaaatgc
     7201 agagtacgct cgttcaagtg aggaacgctt caagcagtat caggctgcta aagaagcctt
     7261 ggctcaagct aacaaacgca aggaaccaga ggaaatcttt gaagaagcgg ctaagttagc
     7321 tgaacaagca cagcaagttc aagcagtggt tgaagtcgtc cctgagaaaa aagaacctgc
     7381 agtggataca cgtcgtaaaa aacaagctcg accagacaaa aatcgtgacg attatgatca
     7441 tgaagaagat ggtcctagaa aacaacaaaa gaatcgaagt agtcaaaatc aagtgagaaa
     7501 tcaaaagaat agtaactgga ataacaacaa aaagaacaaa aaaggcaata acaagaacaa
     7561 ccgtaatcag actccaaaac ctgttacgga gcgtaaattc catgaattgc caacagaatt
     7621 tgaatataca gatggtatga ccgttgcgga aatcgcaaaa cgtatcaaac gtgaaccagc
     7681 tgaaatcgtt aagaaacttt tcatgatggg tgtcatggcc acacaaaacc aatccttgga
     7741 tggggaaaca attgaactcc tcatggtgga ttacggtatc gaagccaaac aaaaggttga
     7801 agtggataat gctgacatcg aacgtttctt tgtcgaagat ggttatctca atgaagatga
     7861 attggttgag cgtccaccag ttgttactat catgggacac gttgaccacg gtaaaacaac
     7921 ccttttggat actcttcgta actcacgtgt tgcgacaggt gaagcaggtg gtattactca
     7981 gcatatcggt gcctaccaaa tcgtggaaaa tggtaagaag attaccttcc ttgatacacc
     8041 aggacacgcg gcctttacat caatgcgtgc gcgtggtgct tctgttaccg atattacgat
     8101 cttggtcgta gcggcagatg acggggttat gcctcagact attgaagcca tcaaccactc
     8161 aaaagcagct aacgttccaa tcatcgtagc cattaacaag attgataaac caggtgctaa
     8221 cccagaacgc gttatcggtg aattggcaga gcatggtgtg atgtcaaccg cttggggtgg
     8281 agattctgaa tttgttgaaa tctcagctaa attcaaccaa aatatcgaag aattgttgga
     8341 aacagtcctt cttgtagctg aaatccaaga actcaaggca gacccaacag tgcgtgcaat
     8401 cggtacggtt atcgaagcgc gcttggataa aggaaaaggt gcggtcgcaa cccttcttgt
     8461 acaacaaggt accttgaatg ttcaagaccc aatcgttgtc ggaaatacct tcggtcgtgt
     8521 ccgtgctatg accaacgacc ttggtcgtcg tgttaaagtt gctggaccat caacaccagt
     8581 ctctatcaca ggtttgaacg aagcaccgat ggcgggtgac cactttgccg tttacgagga
     8641 tgaaaaatct gcgcgtgcag caggtgaaga gcgtgccaaa cgtgccctaa tgaaacaacg
     8701 tcaagctacc caacgtgtta gccttgaaaa cctctttgat acccttaaag ctggggaact
     8761 caaatctgtt aatgttatca tcaaggccga tgtacaaggt tctgttgaag ccctttctgc
     8821 ctcacttcaa aagattgacg tggaaggtgt caaagtgact atcgtccact cagcggtcgg
     8881 tgctatcaac gaatcagatg tgacccttgc cgaagcttca aatgccttta tcgttggttt
     8941 caacgtacgc cctacaccac aagctcgtca acaagcagaa gctgacgatg tggaaatccg
     9001 tcttcacagc attatctaca aggttatcga agagatggaa gaagctatga aagggatgct
     9061 tgatccagaa tttgaagaaa aagttattgg tgaagcggtt atccgtgaaa ccttcaaggt
     9121 gtctaaagtg ggaactatcg gtggatttat ggttatcaac ggtaaggttg cccgtgactc
     9181 taaagtccgt gttatccgtg atggtgtcgt tatctatgat ggtgaactcg caagcttgaa
     9241 acactataaa gacgacgtga aggaagttac aaacggccgt gaaggtggat tgatgatcga
     9301 cggctacaat gatattaaga tggatgatgt gattgaggcg tatgtcatgg aagaaatcaa
     9361 gagataagat tttttgctcc tttcttaggt ggtgagggac gcaagcaaac cgatggtttc
     9421 attgcttatt tttgagccta gggtctcaaa aatcccctgt gatgggactg ataaatcagt
     9481 tccatcactt tcaccacggc gaaagaagcg gatgacttca aattgaactt cgtttcaatt
     9541 taaactgaaa atcaagaagt ttaaaatagc taggtctgct ggcctagctt ttggttcaaa
     9601 gtagagaaag gaatatcatg gcaaatcatt tccgtacaga tcgtgtgggc atggaaatca
     9661 agcgtgaagt caatgagatt ttgcaaaaga aagttcgtga tccacgtgtc caaggtgtga
     9721 ccatcacaga tgttcagatg ttgggtgact tgtctgttgc caaggtttat tacaccattt
     9781 tgagtaacct tgcttcggat aaccaaaaag cccaaatcgg gcttgaaaaa gcaactggta
     9841 ccatcaaacg tgaacttggt cgcaatttga aattgtacaa aatcccagat ttgaccttcg
     9901 tcaaagacga gtccatcgag tatggaaaca agattgacga gatgctacgc aatctggata
     9961 agaactaaag aagaggggtt gcccctcttt tttggtggag gaaaataggt tgaatttgaa
    10021 atggaaaaat attcttttaa aatatattga aactagaata gtacgcctct acttctaaaa
    10081 cattgtta
//
LOCUS       DAVKWS010000065         9748 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKWS010000065 DAVKWS010000000
VERSION     DAVKWS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.066"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            308..403
                     /locus_tag="VW852_001815"
                     /pseudo
     CDS             308..403
                     /locus_tag="VW852_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(656..1276)
                     /locus_tag="VW852_001816"
     CDS             complement(656..1276)
                     /locus_tag="VW852_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0272902.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1276..1758)
                     /locus_tag="VW852_001817"
     CDS             complement(1276..1758)
                     /locus_tag="VW852_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV0272903.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1758..2600)
                     /locus_tag="VW852_001818"
     CDS             complement(1758..2600)
                     /locus_tag="VW852_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV0272904.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2695..3483)
                     /locus_tag="VW852_001819"
     CDS             complement(2695..3483)
                     /locus_tag="VW852_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV0272905.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3627..5006
                     /gene="glmU"
                     /locus_tag="VW852_001820"
     CDS             3627..5006
                     /gene="glmU"
                     /locus_tag="VW852_001820"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV0272906.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLTGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEHYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGSVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            5016..5561
                     /locus_tag="VW852_001821"
     CDS             5016..5561
                     /locus_tag="VW852_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0272907.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5573..5887
                     /locus_tag="VW852_001822"
     CDS             5573..5887
                     /locus_tag="VW852_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV0272908.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5904..6596
                     /locus_tag="VW852_001823"
     CDS             5904..6596
                     /locus_tag="VW852_001823"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV0272909.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQDQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHFGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6673..7164
                     /gene="rocS"
                     /locus_tag="VW852_001824"
     CDS             6673..7164
                     /gene="rocS"
                     /locus_tag="VW852_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV0272910.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7262..7852
                     /locus_tag="VW852_001825"
     CDS             7262..7852
                     /locus_tag="VW852_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV0272911.1"
                     /translation="MERLEDYIAFDLEFNQHEGQKHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     YGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7861..9624
                     /locus_tag="VW852_001826"
     CDS             7861..9624
                     /locus_tag="VW852_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV0272912.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3022 a         1687 c         2066 g         2973 t
ORIGIN      
        1 tgaactttat catcataagt taatttcata ataaaaacac cccaaaagtt agattttttc
       61 tgtctaactt ttggggtgta gttcatgtac acctgatatg atgcgtttta taattttaaa
      121 gactttttga ccagcctcat ttttttaact tgatactcag tgaaaagcaa agattaaact
      181 agaaagctag ccgcaggctg ctcaaagaac agctttgagg ttgcagataa aacttgtgag
      241 gtaaccaaca tatataatgt gaagctgacg tggtttgaat agattttaga agggtatgag
      301 tctggaagtt ttaatggata atgcaagatt ccatagaatg ggtaagctag agttcttatg
      361 tgaagagttt gggcataaac ttttaccttt tcctccctac tcatcttagt atagaaaagt
      421 gaatctaaaa tagtacataa ctgcttctaa aacattctta taaattgatt taaattctca
      481 aatcatatta ttcagttctt atttcatttt gctctacaat cctgttgaga agacacgtgt
      541 tcatatcaaa aaggtattgg caagttgcaa taccttttta caaggttctt ttgtcttatt
      601 tttgtttcaa ctgactatat ctcctatggt tctagttcag aaggctaggc tataattatg
      661 attgataaga agtatcattc caagtattga gagtgaatgt ttcaaaatca tgggtttcta
      721 taatggtcag gctggcattt gctagaccgc catctttacg aagaagtggt tctttatagc
      781 ctaggagagt acgaagactg gcagtaagat tggcgccgtg tccgacaatt agaatacgct
      841 cagctggact atcttttaat gatttgataa attggatggt ccgctgagtt gtactataga
      901 gggattcggc tccgaacatt cgagtgtcaa attgagcaag atttgaacga aaagcctgga
      961 tttgttgcgg gtaaatagct tccagggttg caattttcaa accttctaac ttcccaagtt
     1021 gccattcacg gagattagga acgatttcta aagaacaggg ggtatagagt tgactttgga
     1081 taatctcagc agatttgacc gctcgaggta aatcacttga ataaatctga tcaaaaggaa
     1141 tttccttgag atactgacca agtcgtttta gggtttcaat ggattcagga agaagaggag
     1201 aatcaccact agcaccttga aaacgacctt cttggttcca gagggtacga ccgtggcgga
     1261 caaagtagag tttcattact tgatgtcctc caaaatatct acaaagtctg cctttacaaa
     1321 gctagccaag tcttgtggcg cgacgataat gctgtgtccg acttcgcctg cagagacaat
     1381 catttgatcc aaatctagag caattttatc gataaaaatg ggataattgt gtttctgacg
     1441 aattccgaca ggattattgg ctccatgaat gtaaccagtt gttttttcta agtccttttg
     1501 tggaatcatg ctcacttttt tattgccaga aattttagct agtttctttt cagacaagtg
     1561 ctgagtgata gggacaattc cgataatcgg tccggtcttg tctcccaaaa gcgccaaggt
     1621 tttgaaaatc tgatctcgtt cataaccttg aggaagctct ccttctaggg cattgatttg
     1681 aatcccctga tgagggatag ctgctttaga taggatttgt tccaccaatg tttttttgat
     1741 tttaactttt tttgccatta tttatattta tcctccaatt gactcatcca aataccaagc
     1801 cagattccca gcgcaaagaa gaaggcgatg atgacataac cgacaagtga aagtcctgtg
     1861 tattggatac tttcagcgtt tcctgcattt ggaattaaga tcaaaagggt acttgatagg
     1921 acgataccga tgatgaaatg atagacgcgt gagtggtagt tgtttaaggc ataatccatc
     1981 aattttgaaa aaacgatgag agttgcacct gcaccaattc caatcggaaa gaaggttccc
     2041 aagaaatcaa aagttttaaa accagtcaac ataggagcat atagtcccaa aatcaaaagt
     2101 aaatttgatg ggctgaggcc aggaaccaag acgccaaggg ccaatagtgc gcctgctagg
     2161 acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa aatttaaggc atagagtcct
     2221 aatccagaaa tgataaaggt tgtccataac caagctaaat caatcttgtc tcggtcagat
     2281 tctcgagttg attctttgag gaggctagga actgtaccga taatagctcc cgcaaagctc
     2341 cataatacaa aaacctgata attttcaagc aggtattcaa tcgggtagga aaataagccg
     2401 attcccagaa gcataccgat ggcaactgga ataaagtaca aaacattttc tttaaagtct
     2461 ttaaagggat gggccagaaa gccaatcatt cgttcataga ttcctaagat tgctgctaga
     2521 acccctccgg aaattcccgg taggataaat ccaagagcaa ttacaatccc tttaataacg
     2581 cgtgctaacc atgagagcat atatttcctc caactatttc tatttcaaaa aatccttact
     2641 atattgtatc acaatcagac acaaaaagaa aaagcaaatg ataaacaaat gcttttaagg
     2701 ttttaaaaaa agttttcgaa aggtttcttc tttatttttt aagggagaga taacgttgat
     2761 atctaaatcg tggtcaaagc cggcaatttt tcctttagat gtgtattggt gaatatcata
     2821 atctaaatca gttttaggac tgctctccaa aaatcctgag tctgagccgt aggacggaat
     2881 ccaaacagag gtaaacttgc ctgtatcaat actgtgttct tccatgaagt agacaccaac
     2941 gtagatgccg atgtttttag cacctagtga tgctagtttt gctcgaaagt tttcgacacc
     3001 ttcgttcata ttagacatgg ttttgtcttc cacgtcaagc caatagtaac tagggctgta
     3061 aggagaggca gcattgtaga aaacttcggc agccttttcc atttcttgga cactttttcc
     3121 agctacataa gcgtagacag caactgggac attccgcttt tgaagttcag tgatatgact
     3181 cttataggcc ttgtctattc cattgataaa tgaagcatca ttttcttttg ttgtttgagc
     3241 accactgtga acacgaacaa tagcacctga aatattttgt gagagggcat cgtagttgat
     3301 ttcctcagga cgctgccagc cagagaggtc aataatcggt ttgtctaagt gtttcaaagc
     3361 ctgtgctata ttggattttg ttttaaacga ttggctgtca ttaagtgggc gattgatgat
     3421 taaaatgaac atcataatcc caaaaaaact aaataaaata agtggatgaa tttgttttct
     3481 catatcttat aagtctaccc taaaaatcaa aaaaaatcaa aaaaatgggt taaggaagag
     3541 actttagagc attttttcat tcaagagtgc ggaatgattt gaaatatggt ataataaaag
     3601 ggaatttcta cagaaaagag aagattatgt caaattttgc cattatttta gcagcgggta
     3661 aagggactcg catgaaatct gatttgccaa aagttttgca caaggttgcg ggtatttcta
     3721 tgttggaaca tgttttccgt agtgtgggag ctatccaacc tgaaaagaca gtaacagttg
     3781 taggacacaa ggcagaattg gttgaggagg tcttgactgg acagacagaa tttgtgactc
     3841 aatctgaaca gttgggaact ggtcatgcag ttatgatgac agaacctatc ttagaaggtt
     3901 tgtcaggaca caccttggtc attgcaggag atactccttt aatcactggt gaaagcttga
     3961 aaaacttgat tgatttccat atcaatcata aaaatgtggc cactatcttg actgctgaaa
     4021 cggataatcc ttttggctat ggacgaattg ttcgtaatga caatgctgag gttcttcgta
     4081 ttgttgagca gaaggatgct acagattttg aaaagcaaat caaggaaatc aacactggaa
     4141 catacgtctt tgacaacgag cgtttgtttg aggctttgaa aaatatcaat accaataacg
     4201 ctcaaggcga acactatatt acagacgtca ttggtatttt ccgtgaaact ggtgaaaaag
     4261 ttggcgctta tactttgaaa gattttgatg aaagtcttgg ggtaaatgac cgtgtggcgc
     4321 ttgcgacagc tgagtcagtt atgcgtcgtc gcatcaatca taaacacatg gtcaacggtg
     4381 ttagctttgt caatccagaa gcaacttata tcgatattga tgttgagatt gctccggaag
     4441 ttcaaatcga agccaatgtt accttgaaag ggcaaacgaa aattggtgct gagactgttt
     4501 tgacaaatgg tacttatgta gtggacagca ctatcggagc aggatcggtc attaccaatt
     4561 ctatgattga ggaaagtagt gttgcagacg gtgtgacagt cggtccttat gctcacattc
     4621 gtccaaattc aagtctgggt gcccaagttc atattggtaa ctttgttgag gtgaaaggat
     4681 cttcaatcgg tgagaatacc aaggctggtc atttgactta tatcggaaac tgtgaagtgg
     4741 gaagcaacgt taatttcggt gctggaacta ttacagtcaa ctatgacggc aaaaacaaat
     4801 acaagacagt cattggagac aatgtctttg ttggttcaaa ttcaaccatt attgcaccag
     4861 tagaacttgg tgacaattcc ctcgttggtg ctggttcaac tattactaaa gacgtgccag
     4921 cagatgctat tgctattggt cgcggtcgtc agatcaataa agacgaatat gcaacacgtc
     4981 ttcctcatca tcctaagaac cagtaggagc ctatcatgga gtttgaagaa aaaacgctta
     5041 gccgaaaaga aatctatcaa ggaccaatat ttaaactggt ccaagatcag gttgaattac
     5101 cagaaggcaa gggaactgcc caacgggatt tgattttcca caatggggct gtctgtgttt
     5161 tagcagtaac ggatgaacaa aaacttatct tggtcaagca gtaccgcaaa gctatcgagg
     5221 cagtctctta cgaaattcca gctggaaaat tggaagtagg agaaaacaca gcccctgtgg
     5281 cagctgccct tcgtgaatta gaggaagaaa cagcctatac agggaaatta gaactcttgt
     5341 acgattttta ttcagctatt ggcttttgta atgagaagtt aaaactatat ttagcaagcg
     5401 atttaacaaa agtggaaaat ccgcgtccgc aggatgagga tgaaaccttg gaagtccttg
     5461 aagtgagctt agaagaagcg aaagaattaa tccaatcagg tcatatttgt gatgccaaga
     5521 caattatggc tgttcagtat tgggagttgc agaaaaaata gaggaggtca gtatgggtaa
     5581 atctttatta acggatgaaa tgattgaaag agctaataga ggcgaaaaaa tttcaggtcc
     5641 tcctttgcta gatgataatg aggaaactaa gattttacca acctcttctt cccgttttgg
     5701 ttatgccaat cctaaggatc atggttttag ccaggaaacc ttgaagattc aggtcgaacc
     5761 atctattcat aaaagccgtc gtattgaaaa taccaagaga aatgtcttca attctaagtt
     5821 gaataaaatc ttatttgcgg tcatttttct cttgattttg cttgttttag caatgaaact
     5881 tttgtaatag aaaaggaatt gaaatgaaaa taggaattat tgctgctatg ccagaagaac
     5941 tggcttatct ggtccagcat ttagataatg cccaggatca agttgttttg gggaatacct
     6001 atcatacagg aaccattgct tctcatgaag tcgttcttgt agaaagtgga attggtaagg
     6061 tcatgtctgc tatgagtgtg gcgattttgg ctgatcattt ccaggtggat gcccttatta
     6121 atacgggttc agctggggca gtagcagaag gtatcgctgt tggggatgtc gtgattgctg
     6181 acaaattagc ctatcatgac gtggatgtca cagcttttgg ctatgcttat ggacaaatgg
     6241 cgcaacaacc gctttatttc gaatcagaca aaacctttgt tgctcaaatc caagagagtt
     6301 tatctcaatt ggaccaaaac tggcattttg gtttgattgc tacaggagat agttttgttg
     6361 caggaaatga caagatagaa gcgattaagt cccatttccc agaagtttta gccgtggaga
     6421 tggagggggc agctattgct caagcagcgc atgccctcaa tctcccagtc ttagtcatca
     6481 gagctatgag tgacaatgcc aaccatgaag caaacatctt ttttgatgag tttattatcg
     6541 aagctggacg tcgctctgcc caagtcttgt tgaccttttt gaaggcttta gattaagcgg
     6601 aaatttgaca gtttttctag cttatgataa gatttaagta aagaaaagct agaaaacgtt
     6661 tcagaggata ttatgagtat tgaaatgacc gtcagtgaga ttgcagaggt cttaggatta
     6721 tctcgccaag caatcaataa ccgtgtcaaa gaattaccag aagaagacac agataaaaat
     6781 gacaaagggg taacagttgt taccagaagt ggcttgatta agctagaaga aatctataaa
     6841 aaaacgattt ttgaagatga gcctgtcagt gaagatgtca aacaacgtga actgatggag
     6901 attctagtgg atgagaagaa tgcagaaatt cttcgtcttt atgaacaatt aaaagccaag
     6961 gatcgtcagt tatcagaaaa agacgagcag atgcgtatca aagaccgtca gattgctgag
     7021 aaagacaaac aattagatca gcaacaacaa ttgacccttc aagccatgaa ggatcaagaa
     7081 aatcttaagc tagagctgga ccaagcaaaa gaagaagtcc aatccactaa aaaaggcttt
     7141 tttgctcgtt tatttggagg ataatcaagg agctgtttag gttaaatgct aacctgatgg
     7201 cttcttttgt ttgtgaatag tatagttgat tagaacagag acagtagagg agaggtacag
     7261 aatggaacga ttagaagatt acattgcttt tgacttagaa tttaatcagc acgaaggaca
     7321 aaaacacttg attcaagtat cggcagtgcg ttttagagat ggtcgggaaa tcgatgccta
     7381 tgattcctat gttcatacca gcgtccctct aaaaagcttt atcaatggtt tgaccgggat
     7441 tacagctgag accttaaaag atgctcctcc agttgagaaa gtcttagaag aattccaaga
     7501 gtttgtgggt tctttgctga tgataggcta caatgctgct aaaagtgatt tacctatttt
     7561 agcagagtat ggtttagact atagccaaca gtacaaggtg gatttgtatg atgaagcttt
     7621 tgaacgtcgg agttcagatt tacatggtat tgccaatctc aaattgcaaa ctgttgcctc
     7681 atttcttgga tttcaaggtc agtctcataa tagtttagag gatgctcgga tgacggcgcg
     7741 tgtttacgaa tccttcttag agtcggatca agctagggaa ttgttagaga cagaaagtag
     7801 cttatcaaac aatccttttg gtggcttgga cttgtctcaa ttatttgact aggagtgcct
     7861 atgaaacctg aaaaacctaa aaaattgggt cttaaacaga tttacctcaa tcttgataaa
     7921 atcctctttc tatttttctt gatttttatg atagtggagt atttgtgggt accattcaat
     7981 tcatggttgg ctagcttctt attagatcag acaggttatc tttttatttc ctataacaat
     8041 gtcttagcca tccttactca ttctccattg attagtctag cttttttagc cttgattgta
     8101 attaaccttc tggttgctta ttttcaaata ggactcctct ttattggtgc tcgtcatctc
     8161 ctctataatg aaaagcgaag gctaattgag tatatccgca aggttttcca tgaaagtttt
     8221 ggatttatga aaaagctaac ttgcgctaaa gccttgttta tctttttcta tatagctatg
     8281 ctttttcctt ttatacggaa aatgctgaag atttattatc ttaataaaat cgtcattcca
     8341 gagtttatcc aagcttattt agaagataaa tattggatgt ggtggctgag catcctactt
     8401 ctatctctta tctttctcta tgtctctgtc agattgatgt ttgcccttcc gaagattgta
     8461 tatgatcagc tgactgttcg agaagcagtg atgtttagct tggagaaaac caagaagaga
     8521 gttacttttt atgcttggaa tttattttac atcttgctca aagcacattt attattttat
     8581 cttcctttga ttcctttatt attggctcaa actttggtag atgatatcac tcagagagag
     8641 tctctgattc ttggtatttt gaattttgtc gtgattaaaa atctctatta tatggctctg
     8701 acttattttt tggttaaatt tgtttcattt ttgacaggaa aggagctgga tatgcttcct
     8761 aggagagaaa aagatcacat cgtgcgatgg ggtgtcatga cttgtgccag tcttttcttt
     8821 gccttagaag gttatattta tctggaggct cccatggttc atctacctca acttatttct
     8881 caccgagggg tttccaatgc aaatgggatt caaaatacag tagagtcctt ggaaactaca
     8941 gcacaactca aaccagactt ggtggagacg gacgtgcagg aaacaaaaga tgggcagttt
     9001 gtcatgatgc atgatgctaa cttgaaaaat ctagcaggta tcaataaaag tcctcaagac
     9061 ttaaacttgg aggagcttaa agggattgat atttttgaaa atggctacca gactaaaatt
     9121 tcaagctttg aagattatct cagtcgagcc aacgaacttg gtcaaaaatt actaattgaa
     9181 attaaaacca gtaaaaaaga tagtccagac atgatgaacc gctttttagc ccgttatgct
     9241 gcaaagctca agatttatgg acatcaaatc cagtctttag actaccatgt tgtcgaaaaa
     9301 gtaagacagt atgatgccga actgccagtt tatttcatca tgccctacaa ttctgtcttt
     9361 cctaaaacaa gagcgacagg atatactatg gagtactcaa ccttggatga atattttgta
     9421 agcaaactat ggacaacgga tcagaaactt tatgtctgga ctgtcaatga ttcagaagct
     9481 atcagcaaat ctcttcactt aggagtagat ggagtgatta cagatgattt ggaaaaagtt
     9541 cagcgggaaa tagaagtagc ccaagaagat ccagaatata cggatttgct cttgaaaaaa
     9601 gcattggaat tctttgaatt ttagtaataa aaaagaggaa cggttgttcc tcccatgata
     9661 ggtaagtatt cttgtgaatg cttgcttttt tgtggttgca atccgtttac gagaattgtt
     9721 tgtattgttc ttgtttcaat ttacttta
//
LOCUS       DAVKWS010000066         9489 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKWS010000066 DAVKWS010000000
VERSION     DAVKWS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.067"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..312)
                     /locus_tag="VW852_001827"
     CDS             complement(<1..312)
                     /locus_tag="VW852_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV0272913.1"
                     /translation="MDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARYREI
                     ILAEHDSCIKNDEAVRNFVTSVLLSAFVSALVSAMISLEIQTYKFVIPFIIGMIWTVV
                     VF"
     gene            complement(323..2146)
                     /gene="dnaK"
                     /locus_tag="VW852_001828"
     CDS             complement(323..2146)
                     /gene="dnaK"
                     /locus_tag="VW852_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV0272914.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2626..3150)
                     /gene="grpE"
                     /locus_tag="VW852_001829"
     CDS             complement(2626..3150)
                     /gene="grpE"
                     /locus_tag="VW852_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV0272915.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(3177..4211)
                     /gene="hrcA"
                     /locus_tag="VW852_001830"
     CDS             complement(3177..4211)
                     /gene="hrcA"
                     /locus_tag="VW852_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV0272916.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4375..4812)
                     /locus_tag="VW852_001831"
     CDS             complement(4375..4812)
                     /locus_tag="VW852_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV0272917.1"
                     /translation="MHLKGWRYRTSRFGLFYFEQGQPDDVIYRIYDSRFLKKYKHELQ
                     DFRDRGWELIGAGSCSILRKSSSDLLPEVQVYMSKGLKWEVMRSRLRSCTATFLGGLV
                     VCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFRLKRKYRVDE"
     gene            5491..7824
                     /locus_tag="VW852_001832"
     CDS             5491..7824
                     /locus_tag="VW852_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0272918.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVGEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            7837..9300
                     /locus_tag="VW852_001833"
     CDS             7837..9300
                     /locus_tag="VW852_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0272919.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPKELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9300..>9489
                     /locus_tag="VW852_001834"
     CDS             9300..>9489
                     /locus_tag="VW852_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEV0272920.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         2942 a         1784 c         1851 g         2912 t
ORIGIN      
        1 aaatacaact actgtccaaa tcataccaat tatgaacggt attacaaatt tatatgtttg
       61 tatttctaat gatatcatag ctgataccag cgccgataca aatgcagaca acaatactga
      121 ggtaacaaaa ttccttaccg cttcatcatt tttgatacaa ctatcatgtt cagctaatat
      181 aatttctcta tatctagcta aatattctat tggaatatta tcaataaaat cttttgatgt
      241 tctatagtta tcagcttgat tacaaaaatc attataaact tttcgtgtat tttttagata
      301 ctcttcatcc aatacactcg tcttactttt ccgtaaactc tccgtctacg acgtcatcgc
      361 ctgcgtttcc tgttgcttgt gcgccttctg ctccttcttg agcttgttgc gctgctgcgg
      421 cttgttcgta gagtttaaca gcaagtcctt gagctttttc gttcaatgct tcaagtttcg
      481 ctttcatgtc gtccaagttg ttgtcttctt gagctttctt aaggtcatca agggcagctt
      541 gggcagcgtc acgttctgcg tcgaagcctt taccttcagt ttccttgatt gtcttttcag
      601 tcgcaaagat tgcttggtct acttcattac gaaggtctac ttcttcttta cgtttcttat
      661 cagcttcagc gtttgcttct gcatctttca tcatgcggtc gatttcttcg tcagtcaaac
      721 ctgagttcga ttggatgaca atagtttgtt ctttttgagt tccaaggtct ttggccttaa
      781 cagacacgat accgttcttg tcgatgtcaa atgttacttc gatttgagga attccacgag
      841 gtgcagctgg gatatcagtc aattggaagc gtccaagagt cttgttatct gctgccattg
      901 ggcgttcacc ttgaagaacg tggatatcaa cggctggttg gttgtctgct gctgttgaga
      961 agacttgtga tttagatgtt gggattgtag tgttgcgatc gataagtttt gtaaatactc
     1021 cacccattgt ttcgatacca agtgacaatg gcgttacatc aagaaggaca acgtccttga
     1081 catcaccagt aatcacacca ccttggatag ccgcacccat agcaactact tcatcagggt
     1141 ttactgattt gtttggttct ttaccagttt cagctttaac agcttcaaca acggcaggga
     1201 tacgagttga accaccaaca aggataactt cgtcgatttc tgacaagctc aaacctgcat
     1261 ctgaaagggc ttgacgaact ggaacttttg tacgttcaac aaggtcacga gtcaaatcgt
     1321 caaatttcgc acgagtcaaa gtcatttcca agtgaagagg tccagcctca cctgcagtga
     1381 taaatggcaa gctgatttgt gttgaagtta caccagaaag gtctttcttc gctttttcag
     1441 ccgcatcttt caaacgttgc attgccatct tgtcagtaga caagtcgata ccgttttctt
     1501 tcttgaattc tgctaccaag tggtcaatga ttttttggtc aaagtcgtca ccaccaagtt
     1561 tgttgtcccc tgcagttgac aatacgtcga agacaccgtc acccaattca aggatagaga
     1621 cgtcgaatgt accaccacca aggtcaaata ccaagatttt ttcttctttg tcagtcttgt
     1681 ccaaaccata agcaagagct gctgcagttg gttcgttaac aatacgttct acttcaagac
     1741 cagcaatttt accagcgtct tttgttgctt gacgttgagc gtcgttgaag taagccggaa
     1801 ctgtgataac agctttggtt actttctcac caaggtagtc ttcagcgtag cctttcaagt
     1861 attgaaggat catagctgag atttcttgtg gagtgtattc ttttccattt gcagaaactt
     1921 tttcagaagt tcccatctta gatttgatag agataactgt atctgggttt gtgactgctt
     1981 gacgttttgc agcatcgcca acgataattt ctccgttttt gaatgagact acagatggag
     2041 ttgtgcggtt tccttctggg tttgcgatga ttttgctttc agttccttca agaactgcaa
     2101 ctgctgagtt tgttgtacct aagtcaatac cgataatttt agacatgtgt ttttctcctt
     2161 gttagttaat tttctatttt tctttttgat actcttctta agtggcgacg ccgtcagagc
     2221 ttgacttcgt cagtctcttt gactaaactt tgagcctaag gtctcaaaat ttgcgcaaat
     2281 agcgccacgg cgaagagtat cgtctttggt tcgcttcgct cactattaca aagcaaaaca
     2341 tattttatct ttcttcatag tttattgtcg tttcggacaa gttttctatt atgtaaattg
     2401 cgacacgagg agtcgaaatc gattttattt cgacgacgag ttagtaagga agctaggcaa
     2461 acgccatagc aattgccgtt ttctgacgag ttgctttagc taccgtcaga attgccgaat
     2521 cgaacacgcc ctaagctctg agtaaaaaag atagattttc ttgtgttcat cgaacacttc
     2581 gttaatctcc tatttttact gcgagctttt tacgggcttt gtatcttagt tatacaccac
     2641 taccattgct gggcgtagga tgcggtcatg gagtttgtag cctttttgaa agacttgggc
     2701 gatggtatct actgggtgtt catcgtctgc tgggagagtt tggatggcca tatggtagtt
     2761 atggtcaaat tcgccatctg ctgcgatttc ttcaattcct tcttctttca aagcgtgaat
     2821 caagctttct tgcaccatcc ccaagccctt cttcacatca tctgtcaaac cttcaactgc
     2881 aagtgcacgc tcaaggttgt caagagatgg taagattgct tttgccaagt cctggctacg
     2941 ataacgttgc aagttttgac gttcttcatt ggcacggcgt tggatatttt gcatttctgc
     3001 atgagcgcga agatatttgt tttcgaactc atctgcacgt tcatttgcca agtccaactc
     3061 agacttttca ggagttgttt cttcagctgt ttccacaact tcctcttctt gaacttcttc
     3121 tacttcttca ttttttatat cttgggccat gttcgcctcc tttaatgatt tcaatcttaa
     3181 tgtacttcat aatgattact gctgaggtag cggtaaaaat ctgtcaactt catggtcaaa
     3241 acacggttga ccacattgac ttgattgatt agctgttggt aatccagatt aactggaccg
     3301 ataatggcta gaattccaac tccccgataa ggaatgagga acttactact aatcaccgct
     3361 aggtcagcta aacaggactc ttgaccgtct gcaacacgaa cattttgcat ctgatcctca
     3421 cgcaacccct cacgaatctc caaggccacc ttttgcggtt ggtcaaagaa ctgataggct
     3481 tctagattgg caaaattcaa gagattgacc ttgcccgcca tcacaatgtt ttcgttgaac
     3541 atttccttaa agatgtgttc aaagagatcg atgacattat ccgttgttgt aaagtaacgc
     3601 tggataatct gcggaatctc cgtccgaatc ttgtagtgaa tatctaaaac ggtgtgaccg
     3661 aggaaacgtt cctgaatgat gctcttcagt ttcagcaaat cctcctgcaa gaagttcctt
     3721 ggaatcagaa actgactagt aaccgttcgc gactcgtcta gggtaaatac cgccaaggct
     3781 gtatgttgcc ccaaaacaac gatatcaaag gctgtcaaac gttgcctgct cggctcaaca
     3841 tccagtgcca ctaccgtaca gccactcagg tctgttaaca agttagcagc ctcttgcaga
     3901 atatcctcca atttgaagaa ttcctgatca aaggctttga caatctcata tacctcattt
     3961 tcagccagtc ggtcaaaatc cagtgagtgt ttcacatagt actgaaaacc agcaacactt
     4021 ggcatccgac cgcttgaagt atgagccttc tcaagcaacc cttgtttttc tagttccgcc
     4081 atgtcattac gaatggttgc actgctagag ttaatagact cttgcaaggc ttttgatccg
     4141 acaggttcgt gcgttttggt aaagatgtca ataatcagat ttaaaatatc ctgctgacgc
     4201 tctgtaacca tctcatcact cctctctgtc tgatcaatta gcactcaatg catccaagtg
     4261 ctaacttatg attctattat acacccttag aagagaatgt caagacaaaa actcaaaaaa
     4321 ttagcactct tttataaaga gtgctaaaaa tcaatctgaa gacctagaac cttgctattc
     4381 atctactcgg tatttccttt ttagtctgaa ataaccatga attagataag aaatcagaaa
     4441 agcatataaa acaaaaataa gaaagaaaga catagaaaga tcctctctaa acaaactcat
     4501 acaaacaaca agaccaccta agaaagtagc tgtacaggaa cgaagtctag atcgcataac
     4561 ttcccatttg agccccttac tcatatagac ttgaacctct ggaagtaaat cagaagacga
     4621 tttacgaaga atcgaacaag aacctgctcc tatcaattcc caacctctat ctctaaaatc
     4681 ttgcagttca tgcttatact ttttaagaaa tctagaatca tagatacggt agatgacatc
     4741 gtctggttga ccttgctcaa aatagaacaa accaaaacga ctcgttctat acctccaacc
     4801 tttcaaatgc atctcatgta aatattcttc ttacttgtcc aaatcaggaa tagtgaaaat
     4861 ccgaaattct actctgctat tcattgtctt accccaaaat tagaaaacat gcctggcgtt
     4921 atttattaga taattctttc cacttttgac tcaatctcca aaaaatataa gaaatctgaa
     4981 tcgcaaaaac tattaataaa acccaatcta ttatgaaaat caaaaatact ccccaactga
     5041 aagaactacc tccagtgaca aactttgaga aaaacggtag tagagctaaa aagagaagca
     5101 aaataggaag catccgcatt gttaaaatcc gtttggcata aaaaatcttt atttaaacga
     5161 aaatattatg gcaaaattta cgccagtttt tgaacggctg atgtagatat tttatacttt
     5221 caaaatgttt aaatgtgatt atttattttt gaaaaatagc tcaccagccc gactgaaagt
     5281 gcttatagaa tgataataag tcgcctgccg aaaacagcgg aaaatagcgg tgttatgcgg
     5341 agataatctg acgcgatgcg aaagtatatt gcatacttat tttcaacaat ttagcagagt
     5401 atttttataa gtgtgatata atagaagtat aatttgttct gatagtttat tttatggaga
     5461 agtagatttt tagaatgcgg agggttcaat atggttgagt ttataaagtc taagaaagaa
     5521 atgagtgagg aggatattaa agcaaatttc atcactcctg ctattgtatc caaaggatgg
     5581 aaaaatggtg agcatatcgc ttacgaagaa tacttcactg atggtcgaat tgaggttaga
     5641 ggagataagg ctcgtcgtaa agaaggaaaa aaatcagact attcactgta ttaccaattt
     5701 ggaactcgaa ttgcaattgt tgaggcaaag gataataaac acagcgttcg agcaggatta
     5761 caacaagcta ttgaatatgg agagatttta gatgttccat ttgtttattc ttcgaatggt
     5821 gatggcttta ttgaacacga ccgtatcacg agagaagaac gtgagctgga gttagacgaa
     5881 ttccctactc gtgaagaatt attttctcgt atgacgaagg aaaaaggatt gacgtacgaa
     5941 attacagaag ctatctcaac tccatactat acagacgcct tctcaatgaa aacgccacgc
     6001 tattatcagc aaatagctat caaccgtact attgaaacag ttgccagagg acaaaaacga
     6061 gtaatgtttg tgatggcaac aggaacgggg aaaacgttca tggcttttca aattattcat
     6121 cgccttcgaa aagctggttt ggctaaacga gttttattct tagcagatag aaacatctta
     6181 gtagaccaaa cgatggctga agactttagg ccattcgaaa aggtaatgac gaaaattaca
     6241 ccaaaacttt tgactgctcc tgaaaaatta aattcttttg aaatttatct agggctttat
     6301 cagcaactaa ctggtgaaga tggaactgaa acacattatc aaaaatttga caaagacttc
     6361 tttgatttaa tcgtaattga tgaagcgcac cgtggttcag ctaaggaaga cagtaactgg
     6421 cgtaaggtga ttgattattt cagttctgcg acacagattg ggatgaccgc tactcctaaa
     6481 gaaaccaaga atgcttccaa tacggaatac tttggtgagc caatctatac ttatagttta
     6541 aaacagggaa tcgaggatgg ttttttggct ccatatcgtg ttatgagggt taatttagat
     6601 gtggatgtgg atggttatcg tccagaaact ggaaaagttg atgctaacgg acaattaata
     6661 gaagataggt actacggcag gaaagatttt gataaaacca ttgtcattga tgatagaacg
     6721 caaagagttg ccaagtttgt ttctgattat atgaagcaaa acaatgcacg atttgataaa
     6781 acaattgttt tttgtgttga tattgaccat gccgagcgaa tgcgtgctgc acttgtaaaa
     6841 gagaatctag acttagtcca agaagactat cgttatgtca tgcaagtaac tggtgacaac
     6901 gctgaaggaa aagctcaact ggataacttt atggatgtca attctaattt tcccgctatt
     6961 gtaacaacgt ctaaattatt aacgacagga gttaatgcta aaacatgtcg tttgattgtt
     7021 ttagactcta atatccaatc catgactgaa tttaaacaaa ttattggtcg tggcacacgt
     7081 ctttatcctc aaaaggggaa agaatttttt acgattattg attttcgaaa tgttaccaat
     7141 ttgtttgctg accctgattt tgatggtgag ccagtgaagg tgctggaaac aggtgcgaaa
     7201 acagtcagtg gttctacgcc tggtttcgta ggtgaggaag gtgacccagt agaaaaatat
     7261 atcgttacag acaagcaggt taccattctt aattctactg ttcaagtatt ggatgaaaac
     7321 gggaaactga ttaccgaaag cctgaccgac tacactcgaa agaatatctt aggtagctac
     7381 gccactttga acgattttat cacagtttgg catacggcag ataaaaagaa gcttatctta
     7441 gacgaacttt ataaaaaagg agtttatcta gatgctattc gagagtcgga gggaatatca
     7501 gaacaagaaa tcgatgattt tgatttactc ctaaaacttg cctatggtca aaaagaatta
     7561 accaaaacgg aacgtatcaa taaactcaaa caaagcggat atttatataa atatagtgag
     7621 gaagcgcgtg ctgttttgga aattttactg aacaaataca tggataaagg tattggagaa
     7681 ctcgaaagca ttgaaacatt aaaacttcca gaatttcaga tatatggtgg aaccttcaaa
     7741 atcatcaata cttattttgg agataaaaaa cgatatttac aagcaattaa agaattggag
     7801 caagagctat ttacagtagc ttaatgaaag gaaagtatgt caattacatc atttgtaaaa
     7861 agaattcaag atatcactcg aaacgatgct ggtgttaatg gtgatgctca acgtattgag
     7921 caaatgtctt ggttattatt cttaaaaatt tatgatagcc gtgaaatggt ttgggaatta
     7981 gaagaagacg agtatgagtc aattatccca aaggaattaa aatggcgaaa ttgggctcat
     8041 gctcaaaatg gggaacgggt attgacaggc gatgaattac ttgattttgt caataacaag
     8101 ttattcaaag agttgaaaga gcttgaaata acttcaaata tgcctattcg aaaaacgatt
     8161 gttaaatcag cttttgaaga tgcgaacaac tatatgaaaa atggcgtctt gttacgccaa
     8221 gtcatcaatg ttattgatga agttgatttc aatagccctg aagatcgtca ttcgtttaat
     8281 gatatttacg aaaaaattct taaagatatt caaaatgctg ggaactcagg agaattttat
     8341 acgccacgtg cagcgactga ttttattgcc gaagttcttg acccaaaact tggagaatca
     8401 atggcagacc ttgcttgcgg aacaggaggc ttcttgactt cgactctgaa ccgtttaagt
     8461 agtcaacgta aaactagtga agataccaaa aaatataata cagctgtttt tggtattgaa
     8521 aagaaagcat ttcctcatct tttagcagtt acaaatctgt ttcttcacga aattgatgac
     8581 cctaaaattg ttcatggaaa tactttggag aaaaatgttc gtgaatatac ggatgatgaa
     8641 aaatttgaca ttattatgat gaatccacct tttggagggt cagaattaga aacaataaaa
     8701 aataactttc cagcagaatt acggagttct gaaacagctg atttatttat ggctgtcatt
     8761 atgtatcgtt taaaagaaaa tggtcgtgtt ggagttattt tacctgatgg ttttctattt
     8821 ggtgaaggtg taaaaactcg cttgaaacaa aaactggtag atgagttcaa cttacatacg
     8881 attattaggt tgcctcatag tgtctttgca ccgtatacag gaatccatac gaacattctt
     8941 ttctttgata aaacaaagaa aacagaagaa acttggtttt atcgtttaga tatgccagat
     9001 ggttataaaa atttctcgaa aactaagccg atgaagtcag aacacttcaa tcctgttcgt
     9061 gactggtggg aaaatcgtga agagattctg gaaggtaagt tctacaaatc taaatcattt
     9121 acacctagtg aattggctga gttgaattat aatttagacc agtgtggctt tccaaaagag
     9181 gaagaggaaa tcttaaatcc ctttgagttg attcagaatt atcaagcgga aagagcaact
     9241 ttaaatcata agattgataa tgtattagct gatattttgc agttgttgga ggacaaataa
     9301 tgacaccaga acaacttaaa gcaagtattc tccaaagagc gatggaaggg aaattagtgc
     9361 cgcaaaatcc caatgacgaa cctgcaagtg aattattaaa gagaattaaa gctgaaaaag
     9421 aaaaacttat cagtgaagga aaaatcaaac gagataaaaa ggaaactgag atatttcgtg
     9481 gtgatgatg
//
LOCUS       DAVKWS010000067         8908 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKWS010000067 DAVKWS010000000
VERSION     DAVKWS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.068"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            178..495
                     /locus_tag="VW852_001835"
     CDS             178..495
                     /locus_tag="VW852_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV0272921.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(540..1352)
                     /gene="folK"
                     /locus_tag="VW852_001836"
     CDS             complement(540..1352)
                     /gene="folK"
                     /locus_tag="VW852_001836"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV0272922.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(1395..1949)
                     /gene="folE"
                     /locus_tag="VW852_001837"
     CDS             complement(1395..1949)
                     /gene="folE"
                     /locus_tag="VW852_001837"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV0272923.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(1930..3252)
                     /locus_tag="VW852_001838"
     CDS             complement(1930..3252)
                     /locus_tag="VW852_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0272924.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(3254..4228)
                     /gene="folP"
                     /locus_tag="VW852_001839"
     CDS             complement(3254..4228)
                     /gene="folP"
                     /locus_tag="VW852_001839"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV0272925.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGKFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(4323..5030)
                     /locus_tag="VW852_001840"
     CDS             complement(4323..5030)
                     /locus_tag="VW852_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0272926.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(5083..6501)
                     /locus_tag="VW852_001841"
     CDS             complement(5083..6501)
                     /locus_tag="VW852_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV0272927.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     NVHAMIWILDALFILNYISMAL"
     gene            6691..7503
                     /locus_tag="VW852_001842"
     CDS             6691..7503
                     /locus_tag="VW852_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0272928.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            7842..8861
                     /gene="adhP"
                     /locus_tag="VW852_001843"
     CDS             7842..8861
                     /gene="adhP"
                     /locus_tag="VW852_001843"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV0272929.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         2634 a         2067 c         1729 g         2478 t
ORIGIN      
        1 agtaactgaa agttatataa tcaagttaat ttgggaattt acctattaac ttcggtagat
       61 ttttaggaat tttttcccta tttctccctg taactaaaat ccttttcaaa aaatcacctt
      121 ttcttgattt ttaatcctat ttgctgtatg ataagggaaa agaaagggga cagagatatg
      181 gcttttacca atacccacat gcgatctgct agttttggca ttgttaccag cttgcctgat
      241 gacatcattg actctttttg gtatatcatc gaccatttct taaaaaatgt ctttgaattg
      301 gaagaagaac tcgagtttca attgcttaat aaccaaggaa agattacctt ccacttttca
      361 agtcaacacc tccctacagc cattgatttt gactttaacc atcctttcga ccctcgttat
      421 cccccaagag tactggtttt agacatggac ggtagagaaa ctatcctcct cccagaagaa
      481 aatgacctat tttaaaaact ctagccttca gttgcaagta actgaaaact agagtttttc
      541 tattttttca aagcatcata caagttgcgg atcggttgtt ttaatatcgg atggataaaa
      601 tgaggcgcaa tttcctgtaa ggactcaagg acaaaaaggc gttccgctat gtaaggatga
      661 ggcaatatga ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa gtccaaatca
      721 atcaaacgag gtccccaatg cacttctctc acccgtccca gctctgactc aatggctaac
      781 aaggtttcta acaagtcttg tgctggtagc caggtttcca cctcaaccac ttgattggca
      841 aagctatcct gctccactcc accccaaggc tccgtcgcta agacactgga ctctttgaga
      901 atatggatgc cacgagctcg cagtttgtca atggcttgct tcaagtttgc ttgcttatct
      961 cccatattgc ttcctagggc gataaaggct cgttgcttgc ggcgatgaat ggttaccgag
     1021 caagtatcta gtgacaaatg caccggtgcc caaggttttt tcagttccaa cttcatttct
     1081 tggacaagag gataagactc aaaggtacgt tccaccagtt tataggctac cgtttcaatc
     1141 aaatcctcac tcgtttcctg aaaccaagtc gtccactgct gacacaattc tccgtaatgg
     1201 acagaggctg ttaaatccaa gtctgtagct gccttggtca tatcatagga taggatggct
     1261 gaaacgacaa atttctgccc caattctttc tcactaggaa aaagaccatg ataggcaaac
     1321 atttccaaat ccttaatctg cagttgatcc ataatgattc ctttctagaa aaatccgctt
     1381 gaagcggatt ctttttatag ccccattaaa cgataagctt ggtcgcggag gtccttatct
     1441 gtttcaaata gaccacgagc tactgtcgtc aaggttgcag tgcctggttt tctaacacca
     1501 cgcatactca tacacatatg ttccgcctca atgacaacaa aggctccttt agcacctaga
     1561 tagtccatca aggcatcggc cacttcgata ttcaaacgtt cttgaatttg tggttttttc
     1621 gaataaactt caaccgtacg ggctagctta gacaagcctg ccacacgacc atctggaatg
     1681 taggcaatgt gcgctctacc ataaaatggc aagaagtggt gttcacacat ggtatggaaa
     1741 aagatatcct tttctaccac catattatcg tcaataattt caaaggattt tgacaaatgt
     1801 tcctctgctg tttgaccaag acctgaaaaa atctcttgat acatacgggc tacacgagca
     1861 ggtgtttcct gcaagccctc gcgattagcg tcctctccta cagcctcgat aatcattttc
     1921 acagctgctt caatcttttg tgtatccatt ctcgttcttc ctctccatca gataggccct
     1981 cacttggctc aagaaataca aggaacctgt gacaatccta actgtttgtt tctcttcttt
     2041 tttatctgtc aaattctgct ctagaaaatc atgccaatct tggtagctga gatttctaga
     2101 cttagctgcc tctttcagca cgctttcatc cgtcgcccga ctatccgcaa aatgtgttag
     2161 agtgagctcg gtatctggca tggctcccag caagtccaac atatcctcca aggctttggt
     2221 tttgatacaa gtgaagagga tttccttatg atgatccgca aaacgttctt gcaaggttac
     2281 caacaaggcc ttgatagcat ggggattgtg ggctccatcc aaaatcatca agggatctct
     2341 tgacacgatt tccaaacgcc ctggccaact tgtttcttcc aaggcttgac caagaaagtc
     2401 attgcttgct agctctcgac catcttcttg acaaaaagta tcaagtaaag ctatggccat
     2461 cccagcattc tctatttggt acaaaccaag caggctagtc tggaagcgac cttgtctgac
     2521 agcacttgta tagtcaaaga cttcccctgt caccacactt tcttgatgac gaacctgata
     2581 atctgtcccg taggcaagtc tcggcgcatc tttctcttcc gcaatgcggt caatcacagt
     2641 caaggcttct ggagcaatac gccctgttac caagggcatc ccttgtttga taatacctgc
     2701 cttctgctct gctatggcct ccaaggtgtc accaagtagg gccacatgat ccaagccaat
     2761 agttgtaatt cctgtcaaaa tgggctgaca gacattggta ctatccaaaa gtccacccat
     2821 gccaacttcc atgatggcca catctacttg ctctgaggca aagtagtcat aggccagggc
     2881 tgtgataatc tcaaactcgg ttgtgccctg taaattggcg accgcttctc cctccagcaa
     2941 agactgatag tctgccatga gagcttctag cctcgcttct gagatcgatt ccccattgat
     3001 gctaatctgg tctgtgtaat gaatgagata gggcgagcta aacacgccaa ctctcagccc
     3061 tagcttttct agcatctttt tcaaaaaagc aatagtcgag cccttgccgt tagtccctcc
     3121 gatatggagg accttgagtt tgagatgggg attgccacgc aaagctaaca gttccaccat
     3181 tcgttccaat ccaaaatgcg gttgatccgt ccggtagtta gcaatccact gattgttttc
     3241 aatttctttc atcttattta tattgtttta aatctaaatt ttccgcttca tcagccagac
     3301 gaatggcaga ggcaatttca actgccatcc tgtgactagc tacgtcatgc acgcgtacca
     3361 cttctacacc ctgtctcgca gcgatactag ttacatgagc cgaagccgtg tcccgatttc
     3421 ggaaaccaag ctctgtctca ggattgactt caaaaccatt ctcctctagg atattgatga
     3481 caaatcgctt gcgcgacact ccgagaaaga ttggatagcc cttctgatgt agtttatcca
     3541 ggtcccgtaa aagaagcaga ttttctttct tggtcagacc aaagccaatt cctggatcca
     3601 acaggatatt ttctggtgca ataccagctt ctgccgctct cgctagtgct cgttcaaaga
     3661 aagcctccat caattcttcg attggcaatg tttcaaagtc agctaactct tcctctgtaa
     3721 aagcttgacc aaaaccaaaa tgagggaaga tgagcgagct agggtgctga ggtcgcgcca
     3781 taactggatt aaacatgatg accacttgcg ctctcgcttc agctaccaca tgagccattt
     3841 tctcatcacc cataagacca gtgatatcat tgactagatc ggcaccagca gccaaagcag
     3901 cctctgctac ttgactcttc caagtatcaa tagagatgag gacatcactt tccttgcgaa
     3961 tcgctttgat cactggaaca acacgctgga tttcctcttc tatctcaaca tagctactgc
     4021 ccggccgagt cgattctccg ccgatatcga gcatactggc tccttctgct atcaatttac
     4081 gagcctgctg gagtgcctgc tcaagagcaa aaaatttacc accgtccgaa aaggagtctg
     4141 gggttacatt gataattccg caaataactg tctttgcatg attggcttta cttgacatat
     4201 cggtcactcc ctcaaggatt ttcatcatat tatttttcta ttttaccata aaaagaaaaa
     4261 gatggacacg attgcattca tctttcccta gtagaaagag gtcggttcct acataaaaac
     4321 ttttacacag aaattcctaa tgtccgactc ataatcacca caagagccaa caaacagaaa
     4381 gcaatcccat taacaatcat gtgaagcaag atcgacattt ccaaacgttg ggtcttgtag
     4441 gccgtccaag atagaactgt cgacatacct ccataaatca ataaagaagg taaattactt
     4501 ggttgatgca ataaagcaaa cacaatcgta ccgactacaa atcccaagtt ctccttgcct
     4561 cggaaaatct ttttaggaac aatcccacgg cacaagattt cctcacaaat tggagcaagc
     4621 aaggctagca agaagaagct agaaatcaga gaactattct gaaccatatc gttaatctga
     4681 gactggttag ctgttgtcgt ctcatttgac agttgcaaca agatagaacc aagtatattt
     4741 gaacagataa tgactaaata actcaagccc aaacgtgcca aatccttagt tctaaaaaaa
     4801 gaaaaattaa aacttgccaa ttgcgtttta cgagctccaa taataaataa caccagaacc
     4861 acaattgaaa ggccagcaac tatcagccct gactgtagca gggctacttc ttttaaagtc
     4921 aaaatagagg taaccactag gggaacctga tataaaagga cggcaagtaa aaagaccaaa
     4981 atccagcctg cacgattcaa caattctttc cacatatttt tctctttcat cacctatctc
     5041 ctttgtatta aaaaaggggg gaaatccccc tgggtcattc tattataagg ccatgctgat
     5101 gtagttaagg ataaacaagg catccaaaat ccaaatcatg gcatgaacat ttttaacttg
     5161 acctttgaca agcttagtca aagtgtaagt caagaaacca actgcaatcc cttgagtgat
     5221 agagtagctg aatcccataa agatagatgt gaagaaggca ggaactgctt cagacatatc
     5281 atcccaatgg atatttttca agctaccaag catcataatc ccaacgataa tcaagattgg
     5341 agctgtagcc gctgttggta cgatcgctag aagtgggcta aagaagcttg aaatcgcaaa
     5401 acagatagct acaaccaagg ctgtcaaacc agtacgtcca cctgcaccga taccagcagc
     5461 agactcaaca taagtcgtta cgtttgaagt acctgcaatg gcaccgactg tcgttccaat
     5521 caaatcagag taaagagcct tatccaattt ggctgattgg tgattttcac catttgtcgc
     5581 tacaatacca actttttcac ctgtaccgat caaggtacca attgtgtcaa aaatatctgt
     5641 caatgagaag gcaagaatgg ccatcagagt ttcaggcaag cgagctgtat ctgaaaccaa
     5701 agctcctaaa ccttctgaac caagagctgc accaaagatt gtcttcaaat cttcaaaagc
     5761 tgcaccaaca tggttattag caaaatcgat actagacaaa tcaaccaaac caactgcaat
     5821 agcaagaaca gttgttgtca agatagagag aataattccc cctttaatcc ctttgatgac
     5881 aaagaagata gtaatggcaa gtcctgcaag agccactaaa acagctggat tattaaagct
     5941 gaccaatcct ggaactgctg aagagtttgc tgcaatcgtt gcttgagctt tgtcagcccc
     6001 ttctcctaca acagtatagt tgcctggatc aatcgtgaat ttcaaaagtc cagcattctt
     6061 aatccctaca taggcaagga agacaccgat accagctgaa atagctgagc gaagagcatt
     6121 gggaatcgat tcaatgatca ttttacgaac atttgtcaag gtaataatca atgaaataat
     6181 cccacagatg aagaccatag ctagggcttc ttgccaagaa taaccaagcc cgaatacaac
     6241 tgtaaaggta aagaaggcat tgagtcccat acctggcgct tgggcataag gtaagttagc
     6301 ataaaaagcc atcatcaagg tacccgctac tgcaccaata atcgtcgcta ggaagacgcc
     6361 ctgagcaggc attcctgttt gtgaaagtat ttgtgggttt acaaagagaa tatagctcat
     6421 tgcaaagaaa gttgttaaac cagcgagaac ctctgtacga acgtctgtac cgttctcttt
     6481 tagtttaaat aatttgtcca tcatcaatct ccttttaatt tttacgaaca ataatagaat
     6541 tatatcattt atcattcact ttttcaatgt ctttgtttga tttgtttaag aaattaatcc
     6601 aatttcattt tttgtttgga atctgctttt ctgcctgctt tctgttataa tagaagacat
     6661 cttatctgaa aacacactag aaaggtccat atgacgaaaa aaattattgc agttgacctg
     6721 gatggaaccc tgctcaactc agacagtcaa atttctgact ttaccaaaaa aaccatcaaa
     6781 aaagttgctg aaaaaggcca tcaggttatt attacgacag gtcgccctta ccgtatgtca
     6841 aaagattttt accgtgaact gggcttagac actcctatga ttaacttcaa cggatccctt
     6901 actcatttac cagaccaagt ttgggatttt gaaaagtgtt tgactgtaga caaaaaatat
     6961 ctgctagata tggttcaacg ttcagaggac attcaagccg attttatcgc tggagaatat
     7021 cgtaaaaaat tctacattac aaatcccaat gaagaaattg ccaatcccaa actatttggt
     7081 gtagaagctt tccagcctga agatcaattc cagcctgaat tggtgaccaa ggaccctaac
     7141 tgtatcctct tgcagaccag agccagtgac aaatatgcct tggcaaaaga catgaacgcc
     7201 ttctaccagc atcaactttc tatcaatacc tggggaggtc cgctcaatat ccttgaatgt
     7261 accccaaaag gtgtcaacaa ggcctttgct ttggactact tgctcaagat aatgaatcgt
     7321 gacaaaaaag atttgattgc ctttggagat gaacacaatg ataccgaaat gctcgctttt
     7381 gctgggaagg gttatgccat gaaaaatgcc aatccagagc tactccctta tgcagatgag
     7441 caaatttccc ttaccaacga ccaagatggg gttgccaaaa ccctacaaga cttattctta
     7501 taacctatac tgatactcaa tgagaatcaa agagcaaact aggaagctag ccgtaggttg
     7561 ctcaaagcac tgctttgagg ttgtagatga agctgacgtg gtttgaagag attttcgaag
     7621 agtatgacca gcttgcaagc tggtctttgt gctcttttca cagtctcatc aaccagttaa
     7681 tcgattgttc tactttttgt ctccaaaacc ttggaaaagg ctttcttttg tgatatactt
     7741 cacatataaa tacaagagag ctcgtcacac tcgactctgt tgacaaaaaa tcaatccagt
     7801 cccgttgtcc ctgttctcgg tcaatatcaa ggaggatagc tatgaaagct gttgttgtaa
     7861 atccagaaag cactggtgtt gctattgaag aaaaagtact ccgtccactt gaaactgggg
     7921 aagcacttgt agaagttgaa tactgtggcg tttgccacac cgacctccac gttgctcatg
     7981 gtgactttgg tcaagtccca ggacgtgttc ttgggcacga aggtatcggt atcgttaaag
     8041 agattgctcc agatgtgaaa agccttaaag tcggtgaccg cgtcagcgtt gcttggttct
     8101 ttgaaggatg tggcacttgc gaatactgta caactggtcg cgaaaccctt tgccgtacag
     8161 tgaaaaatgc tggctactca gtagacggtg gtatggctga acaatgtatc gtaactgctg
     8221 actatgctgt caaggttcct gacggacttg atccagccca agcttcttct atcacatgtg
     8281 ctggagtaac aacctataaa gctatcaaag aagcaaaagt tgaaccaggc caatgggttg
     8341 ttctttacgg tgctggtgga cttggtaacc tcgctgttca atacgctaaa aaagtattca
     8401 atgctcatgt tatcgcagtc gatatcaaca atgacaaact tgcccttgca aaagaagtag
     8461 gcgctgacat tgtgattaac ggcctcgaag ttgaagatgt agctggactc atcaaagaaa
     8521 aaactgatgg aggagctcat tcagctgtcg taactgctgt gtctaaagtt gccttcaacc
     8581 aggctgttga ctccgttcgt gctggtggtc gcgtcgtcgc tgttggtctt ccttctgaaa
     8641 tgatggaact cagcatcgtt aaaacagtcc tcgatggaat ccaagtcatc ggttctcttg
     8701 tcggaactcg taaagactta gaagaagcct tccaatttgg tgcagaaggt ctggtagccc
     8761 cagttgttca aaaacgtcca gtagaagatg ctgttgccat tttcgacgaa atggaaaaag
     8821 gtcaaatcca aggacgtatg gtactcgact tcacccacta atctaataga aacctatttt
     8881 aaaagttgga agcttcttcc aacttttt
//
LOCUS       DAVKWS010000068         8417 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKWS010000068 DAVKWS010000000
VERSION     DAVKWS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.069"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(106..309)
                     /locus_tag="VW852_001844"
     CDS             complement(106..309)
                     /locus_tag="VW852_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272930.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(302..1384)
                     /locus_tag="VW852_001845"
     CDS             complement(302..1384)
                     /locus_tag="VW852_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0272931.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1510..2292)
                     /locus_tag="VW852_001846"
     CDS             complement(1510..2292)
                     /locus_tag="VW852_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV0272932.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2292..3635)
                     /locus_tag="VW852_001847"
     CDS             complement(2292..3635)
                     /locus_tag="VW852_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV0272933.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKKCTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3734..5050)
                     /locus_tag="VW852_001848"
     CDS             complement(3734..5050)
                     /locus_tag="VW852_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV0272934.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFTGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5243..6469
                     /locus_tag="VW852_001849"
     CDS             5243..6469
                     /locus_tag="VW852_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV0272935.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLLFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6668..8242
                     /locus_tag="VW852_001850"
     CDS             6668..8242
                     /locus_tag="VW852_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0272936.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2413 a         1628 c         1865 g         2511 t
ORIGIN      
        1 gggggcagta cacaatacct gatagtatgc gttttattgt gggaagattt tcctcatttt
       61 ctagtgaaat tgaacttttt ctaacatctt ttgatagatg acttcttaat caattatcat
      121 ttccattgtt gtttcttggg gtttcatccc ttgtctttga taaaagcgga gagcaccctc
      181 gttatcattc cagacactta gggttagatt attgcagcca atttctttag cataatagca
      241 gaaaaatgac aataaaatag cccctagata ttttgaaagg aaagcatagg ggctatatag
      301 tttaatcaaa ataaagcaaa tctttgctgg tgcttgtaaa gaggtcgaag ccatccttgg
      361 taacaacacc gcagtcttca atacgaacac cgactttacc agggatatag atacctggtt
      421 caacagagaa gcacatgcct tcttcgatga ccatgtcgtt tccttccatg atagatggga
      481 attcatggac atccataccg ataccatgcc cgagacggtg attgaagtac tcaccataac
      541 cagctttttc gatgacctca cgggcagcgc ggtccacttc atgagcagtc acacctggct
      601 tgataaagtc aagagcagct tgttgggctt caagagtcaa gttgtaaata tctttcttga
      661 attggtctgg tttgccgaca gcgactgtac gagtcatatc tgacgcatag ccattgacca
      721 gaacacccag gtcaaagagg agaagagcat cattttcaac cttattagct gctggaatgc
      781 cgtgtggatt cgcagcatta tcaccagtca agaccatggt atcaaagctc atttcataac
      841 cttcacgttt catggcaaag tcgatttgtg cgatgatatc tgtctcagtc ttatcaagag
      901 aaatattgtc aaaaccaaca tgaacagcct tgtcagcata aagacctgca accatcattt
      961 tttgcacttc atcagctgat ttgatgaggc gcatgcgttg gatacgagga gtgaggttgt
     1021 caaactcagc agtctcaaaa actgttttca aaccatggta tttggtcaag atgagattgt
     1081 caaactcaac agcgacacgt ttgaagtcaa gttgtggaag agcatgtttg attttttgcc
     1141 atggattttc agaatcgaca tagcccacta ctgggaagga aacggtgcta cttgcacgtt
     1201 ctacttcaag agctgggaca aagaggagag gttcctgatc cgctaggaca aagaggaaca
     1261 tttggcgttc atggggatca ctgtaaaaac cagtgaggta attgattgtg acggggtcag
     1321 atacgacagc gacgtctagt ttttctgatt caagatatgt taggatttgt tgtaatttag
     1381 acatatgcta ccttctttct acccctctat tttggcaaaa aatgagagaa aatgcaaggg
     1441 agaagggtaa aagaaaagac aaaaaactcc gacaagataa cggagttctt tgatatcatt
     1501 ttcctttgtt taagaaaagt cagccttgct tttgacgtca ctgtactctt cagcgatttc
     1561 ttggctgagg atatcctcat aggcagggag ttggatatcc tgaccatatt tcttcttgag
     1621 ggcagtagta actaggcgat aagccagatt ggcattacga gagaggatag gcccgtggaa
     1681 gtaggaacca aagacattct tataatgaac cccttcaccg accttttctt cgttgtttcc
     1741 atttccatag acaacctgtc ccagcggttt ttggtcatca gagaggaagg tacggccttg
     1801 gtgattttca aatccatagt aggtttcatc gaaatcttca ttgtgaatct tgatgtcacc
     1861 gataaaacgg ttattggtct ggttgagcgt gtagtgtccc atgaccccta gcccttcgat
     1921 acgttttcct gaagcttcaa cataatattg acccaatagt tggaaaccac cgcagatagc
     1981 cagaactaca ccgtcgtttt ggatgtagtt gtcaatgctc tcttttttag caggtaggtc
     2041 gtctgcaatg atactttgtt caaagtcttg accaccaccg aaaaaggcga tgtcgtagtg
     2101 attttcatca aagtcatcat ggagagaaac gatgtcaacg gtcacatggg ctcccagttt
     2161 ttcagccaca tatttgagca tgaggatgtt tccattgtcc ccgtaggtat tcatgagatt
     2221 tccgtagagg tgggcaatgt tgagctgata ggggtaattg ccatcttttg aggaaagtga
     2281 agtataaacc attagttcat ctcctttcta acaatctgac gactagccag cagttcacga
     2341 aattctagca tggcagtata agttgccaga atataggcat gcttgcagtc ttgattctca
     2401 atggtcttga gaacttgctc cagattactc gtttcagtga ttttctcagc tggatagcca
     2461 gtcactcgga ggcgacgagc gatttcagaa tgacgaacac cgccagcgtt gatttcagga
     2521 atgtccatgt cagtgatttg ctcaaagtct gcatcccaga tccagctagt gtcaattcca
     2581 tctgcatagt tggcattaag gaggacagat aggctaaatg gataaggtgc tagtttgatc
     2641 atttcgatag cttgggttgc accgactgga tttttaatca agacaagggt acatttcttg
     2701 tcaccgatat gaaaggtttc ttggcgtcca aagacagcac ggctcttgtc aaatccctgt
     2761 ttgatgagtt gcgaatcggc acctaggaaa cgggcgatgg ccacagcagc tagggcgtta
     2821 tagatattat agagcccgcc gatttggata ccgtattctt ggccgtctat gacaaagcga
     2881 gagcgattgt tggtcaactc aaccagtttt gtcaaacgat agtcgagatc aggacgttta
     2941 catccacaac cttcacagat ataggcaccc aagtttgcat aggtattatg ctcatatttg
     3001 aggatgcctt ggcagtcagg acagagaatc ccttcggtat tgtagtgagc cagttgggct
     3061 ggtccttttt ccaagtcaaa accaaaatac tctatagggt ttggaatagt tggcttgtag
     3121 aaaagtggac tgtctccgtt aaggagaaca gtagcagttg gaacttttcg aatggcatcc
     3181 aatatcatgt tataggtagt atagatttca ccgaaacggt ccatctggtc acggaagata
     3241 ttagtaatga caaaaagact aggctggata tagtcacaga tacgagatag actggcttcg
     3301 tcaatttcga ggacggcaat atttttccca gtttttgaag atttggctgt taggaaggtt
     3361 gttgcaatcc ctgtaatcat gttggcaccg cttgggttgg ttagaacttg accataaacc
     3421 tcttttaaaa tgccgacagt gagggcagtt gtcagggttt ttccatttgt tccagtgaca
     3481 acgacaatct cgtagttctt agctaggttt tgtaaaatat ctttatcaaa ttgaagggcg
     3541 actttacctg ggagcgtact tccacgtcca agacggctta aaacgaagtg ggaagaacgc
     3601 ccagcaagaa ggcccaaagt agtttttaag ttcatgtttc tattatatca caaaagaggg
     3661 aaaagacaga tttggtgttt cgtagaagta aaaacagccc aaagagggct gtcagttaag
     3721 atgagatttc aacttaaatc gcaaacaagt cattcaagtt ctcagccaag gttgggtgag
     3781 tgaagatttg ttttgtgaag taagtgtaag gaatcttgtt gtccatagca acagtaatga
     3841 tgttgatgat ttcttgagaa ccttctgaga agatgcttgc accaagaatt tcttttgttt
     3901 cagtattaac aacagctttg aaagctccgc gaaggtctcc atttacgtga ccacgaggca
     3961 tggctgcaac agggatttct ttcactgcgt atggaagttt caaatcagct gcttggcttt
     4021 cagtcaaacc aacttgtgaa agtgcaggtg tgatgaacat agtatttggc acattgagac
     4081 ggtcctcaag tgtgtagctg ccatctccag caaggtagct gtaaacaaca cggaagtcat
     4141 caagtgaaat gtaagtaaat tgaagaccac cgttgacatc tccaactgca aagacaccag
     4201 gaacgtttgt ttgacagtgt ttatctactt taatagcgcc acgttcagtt agttcaatat
     4261 ctgtattttc aagttgaagt ggttctacat ttggtttgcg tccagttgcg tagagaaggg
     4321 cgtcgaaacg gtaagtttcg tcttcagtta cgacaagcac ttggtcaccg tcgtttttaa
     4381 tttcagtagt atggatattt tgaagcaatt caataccgtc ttcttccatg tattgtttag
     4441 caagagctgc gatggaaggt tctgcacgag gtaggaatgt atccaaggca tctaggactg
     4501 taaccttgct tcctagttta ttgtaaaggc cagtaaattc aagaccgata tttccgccac
     4561 caaggactcc aagtttttca ggcaatttat ccaagctttg gatacctgtt gagtcaaaga
     4621 cgtttttgct tgtagcaagt ccagggattg gcaagacgtt tgaaacagca ccagtgttga
     4681 tgacgatagt ttcagcagtc agttctttct tttcatcacc agcttggatt tcgatgactt
     4741 tatttgaaag gaagtgagct tccgcatcaa agatatctac gcctgtacca gcaacagtcg
     4801 catagttttt accgttgagg cgaccagtga tcgtgttttt agtagcaatc acttcttcaa
     4861 aagacaagtc cttttcagca gcaactagca aggttttagt tgggatacaa ccaatgttga
     4921 tacaagttcc accgtacata gccttgctgc gttcaacgag ggcaactttt ttgccagctg
     4981 aagccaattt acctgctagt gttttaccag ctttaccaaa tccaataaca attaaatcgt
     5041 atgttaacat ttatcttcct cctattcgaa tttcattcta gcacaagaaa aaaacttttg
     5101 cacaattctg ctacggaaaa gacagaaaaa gataaaaacg ctttccagac aaaaaactag
     5161 taattttttg acaagcttga taaactaaaa tcctatctat ttttatataa gatagaataa
     5221 atgttcgaaa ataggaggtt ttatgaaatc gaatcgttat attattgcct ttgctggggt
     5281 cattttacac ttaatgctgg gttcgactta tgcttggagt gtttatcgta accctattat
     5341 tgaaaaaacg ggatgggatc aggcttctgt tgcctttgcc tttagtctag caatcttttg
     5401 tttgggctta tcggctgcat ttatggggcg tttggtagaa aaatttggtc cgagagtcat
     5461 ggggagtcta tctgcttttc tatacgcagg tggaaatatc ttaacaggat ttgcaataga
     5521 ccgtcaggaa ctgtggttgt tgtatctagc ttatggcatt ttaggtgggc ttggtttggg
     5581 agcaggctat attacccctg tgtcaacgat tataaaatgg tttcctgata aacgtggtct
     5641 cgcaacaggt ttagcgatta tggggtttgg ttttgcttct ttattgacta gtcccatagc
     5701 gcaacatctc atcgcagggg tagggcttgt agaaactttt tatattttag gtgcaagtta
     5761 ctttattatc atgctcctag cttcacaatt cattaagcgt ccaaatgagc aagagcttgc
     5821 aattttatct gcttcaggga aagaaaaaac agactctctg acgcaaggga tgactgcaaa
     5881 tcaggctcta aaaagcaatc ggttttatat actttggatt attttcttta tcaacatagc
     5941 ttgtggttta ggcttaattt cagcggcatc gccaatggca caggagatgg ctggcttgtc
     6001 tacaagtcat gcagcagtaa tggtgggcgt tttagggatt ttcaatggat ttggtcgctt
     6061 gctctgggcg agcttatctg actatatcgg tcgccctcta acctttagta tattactgct
     6121 tgttaatctt ttattttctc tctcactttg gctctttaca gattccgttt tatttgtagt
     6181 cgctatgtct attttgatga cttgctatgg agctggtttt tcattgatcc cagcttatct
     6241 cagtgatatt tttggaacca aggaattggc cgctctgcat ggatatattt taacagcttg
     6301 ggcaatggct ggtttagcgg gacctatttt attagcagag acttataaaa tggctcattc
     6361 gtacacacaa accttgttcg tttttctcat tttatacagt atcgccttgg ctttgtctta
     6421 ttatctaggt cgttcaatca aaaaagaaag tcaaaaaccg cttacatgat tttctgtaga
     6481 aatggggcta tcttttgctc tggtcttgtg ttatactaga taggttgcaa agaaaacagt
     6541 acttttcttt tgtggaaaag aagcaacacg attttatatc tagttatatg aaaatacgtc
     6601 ataaaaagaa aagtataaac taagccctat agggtggctt cgcaccacct ttagaaagaa
     6661 gaataacgtg aaatttaatg aattaaactt gtctgctgat ttgctggcag aaattgaaaa
     6721 agctggtttt gtagaagcta gtcctatcca agaacaaact attcctttgg cccttgaagg
     6781 caaggatgtt atcggtcaag ctcagactgg tacaggaaaa actgcagcct ttggcttgcc
     6841 tacccttgaa aaaatccgta cagaagaagc gactatccaa gccttggtca tcgctccaac
     6901 tcgtgaacta gctgtccaaa gtcaagaaga actcttccgc tttggtcgta gtaagggagt
     6961 caaagtccgt tcagtatatg gcggatcaag cattgaaaaa caaattaagg ctcttaaatc
     7021 tggtgcccat atcgtggtgg gaactccagg tcgcctcttg gacttgatta aacgcaaggc
     7081 cttgaaatta caaaacattg aaacccttat ccttgacgaa gcggatgaaa tgcttaacat
     7141 gggcttcctt gaagacatcg aagccattat ttcccgtgta cctgagaacc gtcaaacttt
     7201 gcttttctca gcaactatgc cagatgccat caaacgtatc ggtgttcagt ttatgaaagc
     7261 ccctgaacat gtcaagattg cggctaagga attgacaaca gaattggttg accagtacta
     7321 tatccgtgtt aaggaacaag aaaaatttga caccatgact cgtctcatgg atgtggcaca
     7381 accagaactc gctattgtat ttggtcgtac caaacgccgt gtggatgaat tgactcgtgg
     7441 tttgaaaatt cgtggcttcc gtgcagaagg aattcatggc gacctagacc aaaacaaacg
     7501 tcttcgtgtc cttcgtgact ttaaaaatgg caatcttgat gttttggttg cgacagacgt
     7561 tgcagcgcgt ggtttggata tttcaggtgt gacccatgtc tacaactacg atattccaca
     7621 agatcctgag agttatgttc accgtatcgg tcgtacaggt cgtgctggta agtcaggtca
     7681 atctattact tttgttgctc caaacgaaat gggttacctt caaatcattg aaaacttgac
     7741 taagaaacgc atgaaaggtc tcaaacctgc aagtgtagaa gaatccttcc aatcaaaaaa
     7801 acaggtggct ctcaagaaaa tcgaacgtga ttttgcagat gaaaccattc gtgccaactt
     7861 tgagaaattt ggtaaggatg ctcgcaaatt ggctgctgag tttactccag aagaattggc
     7921 aatgtatatc ttgagtctga cagtccaaga cccagatagc cttccagaag tggagattgc
     7981 acgtgaaaaa ccactaccgt ttaaaccatc aggtaatggt ttcggtggta aagctaaggg
     8041 aggtcgtgga ggccgtcgtg gggacgaccg tcgagagcgt gatcgccgtg gcaatggtcg
     8101 ccgtgatgag ttcaaaaaag gaagtcgtgg caacgatcgt tttgataagg aaaaacgtta
     8161 ccgtaaggat aataaaaaac cacgcaatac tttaagcgaa aagcaaacag gctttgttat
     8221 tcgtaacaaa ggtgataaat aagaaaaagc ggttcaaaat gaatcgcttt tttatataag
     8281 gagaccgaat gtgaaaagac aaataaaatt actatattat cttttataat attataatac
     8341 acaaaaatag gcgacttgtc aatagaaaag gaacaattta attaaaaata ttctggtttt
     8401 taattaaatt gtaagtc
//
LOCUS       DAVKWS010000069         8291 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKWS010000069 DAVKWS010000000
VERSION     DAVKWS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.070"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..1676
                     /locus_tag="VW852_001851"
     CDS             <1..1676
                     /locus_tag="VW852_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0272937.1"
                     /translation="KEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGE
                     YEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQD
                     YGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNGGF
                     APYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKN
                     PLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVS
                     DEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGT
                     KYKDFYYYGYKHRQMIRGHKCTFSKQIREEFLDDAVAEVIVKIVSNPKFASMMQEKIN
                     MKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMY
                     DKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALIS
                     EIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1944..2510
                     /locus_tag="VW852_001852"
     CDS             1944..2510
                     /locus_tag="VW852_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272938.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2831..3019
                     /locus_tag="VW852_001853"
     CDS             2831..3019
                     /locus_tag="VW852_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV0272939.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3081..3839
                     /locus_tag="VW852_001854"
     CDS             3081..3839
                     /locus_tag="VW852_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0272940.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            3897..4751
                     /locus_tag="VW852_001855"
     CDS             3897..4751
                     /locus_tag="VW852_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV0272941.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            4863..5747
                     /locus_tag="VW852_001856"
     CDS             4863..5747
                     /locus_tag="VW852_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV0272942.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            5910..7037
                     /locus_tag="VW852_001857"
     CDS             5910..7037
                     /locus_tag="VW852_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV0272943.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLFEIIKNIKNLVINLN"
     gene            6986..8291
                     /locus_tag="VW852_001858"
                     /pseudo
     CDS             6986..8291
                     /locus_tag="VW852_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         2944 a         1156 c         1734 g         2457 t
ORIGIN      
        1 caaaagaaaa aataaaagta tacctctata cacgagtatc tacgtcaata cagatagagg
       61 ggtattcgtt agaggcacaa aaatcacgaa tgaaagcttt tgctatttac aatgattatg
      121 aaattgttgg agaatatgaa gatgccggga agtctggcaa gtctattgaa ggtagaatac
      181 agttcaatcg aatgatggaa gatataaaat ctggaaaaga tggagtatct tttgttcttg
      241 tgtttaagtt atcaagattt gcaagaaatg ccgctgatgt tctttctaca cttcagataa
      301 tgcaggatta tggagttaat cttatctgcg ttgaagatgg cattgattca tctaaagatg
      361 ctgggaaact aatgatttct gttttatcag ctgtggctga aatagaaaga gaaaacatcc
      421 gtattcaaac aatggaaggt cgcattcaaa aagcaaggga aggaaaatgg aatggtggct
      481 ttgctccgta tggatataaa cttgaagacg gcaagctgtt tataaatgag gaagaggcag
      541 ttgcaataag aacgattttc gaccagtatg taaatactac gataggagcc aatgggatat
      601 ctaaatactt agagaatcat ggaattagaa aaatcccaag acagaatggt aagaatcctt
      661 tgtttgatgc aggtcttata agaaagatat taaagaatcc tgtatataat gggaaaatag
      721 catttggaag aagaacttta gaaaaagttc atggtacaag aaatgaatat aagcaggttg
      781 aacaagatga atatctaata tctgaaggga tacatgaagc tatagtttcc gatgaagttt
      841 ggcaagctgc tcaggttaag ctaaaatctc aagcaaagaa atatgagcat gtgaataaag
      901 gaaaagatac acgcacacac ttgctttctg gaattgtaaa atgcccgata tgtggagtgg
      961 gaatgtttgg gaacaagtgt atcaagaaaa agaaagatgg cacaaagtat aaagattttt
     1021 attactatgg ttataagcat aggcagatga taagaggtca taagtgtaca ttcagtaagc
     1081 aaattagaga agaattttta gatgatgctg ttgcagaggt gattgtcaag atagtaagta
     1141 atccgaaatt tgcttctatg atgcaagaaa aaatcaacat gaaggtggat acctctgaaa
     1201 tagaaaaaga aatagataat taccaaaagg aattgagaaa gagtcattct acaaagttta
     1261 agctaattga ggaaatagat aatttagatg ttgaagataa gcattataag agaaggaaac
     1321 aggatttaga tgatagacta tatcgtatgt atgacaaaat agatgaatta gaatcatcac
     1381 taattgatgc gaaagcaaag aaacagacta ttgaagctga aaagcttaca ggagataaca
     1441 tatataaggt tctgatctat tttgataagc tctataaagt catgaatgat gtagagcgta
     1501 ggcagttaat ttcagctttg atttctgaaa ttcaagttta tgaagaaaaa caatctaacg
     1561 gacaatggct aaaatccatt acttttaagc taccaattat agaagaaaat ctcaacatag
     1621 gtttggataa tgatgaacaa gttgaatgtg taagtttatt agagaaacgt agttaatcct
     1681 caaaaggttc cccaaacctt ttgagtccca ctgacgcatc acgctgaggc ggtatcactg
     1741 cttgtacgag ttgaagcatc agcgaagtaa aagtagatgt tcagccaatg cgacgaggtg
     1801 agaccgaagg tcgataaggt gtgttctggg tataggactc gtaaagtaag taggaaatat
     1861 gatgagatga tacagagagc ctgaattggt caggtgacgt gcttatttac attagcctga
     1921 taatgaaaat ggagagatta tcgatggtta tattggaatt gtaccaaaat gattattcta
     1981 aagatttagt agcatttgat tccatagaag atgggaaagc ctttgtagct caaatcccgg
     2041 gatacactct ggaaacagaa gatggttttg aagtggagta ttttaatcct aaaaacatac
     2101 cggattatat ggaaattatt tttaacggaa atattgttcc tttatctaaa tttatgttcg
     2161 atcctgagga aaatgtaaac attatttgga aagagatttc aaatttatcc ctcaaaaatg
     2221 atagagtgat cgaaggatat tcaaaaattg atgcatatgt cgttaacaac catgaagtta
     2281 aagcctatgt cgaaacaaga gaaacgaact atcgtaaagc aaaagacttc ttagaaagcc
     2341 gcggatatga aatcgacaga agctttttcg gaagtgaaga cggtgaagcg attctttaca
     2401 gaaaaaaagg gatcgaagtt tggcatttct tgtgtcactt agacccaatg tttgtagaga
     2461 ttgaagatgt agaaggatat gttaaagaag aagttggaga gattcaatag tcaaccaaag
     2521 ttccttgtga gtatatacct ttgctagtaa aagagtcaaa acgttataac gaagggggga
     2581 agaaaaccct tgagtgtaag tctttttctt gtttagaggg aaagtattag agtagccgct
     2641 cataatttat actagatttg ataactgatt tgagacagat tttatttcta taaatattgt
     2701 gaaaattttt taataccaaa gatttaaagt tactgtgtta aaaatcctct ccacttttaa
     2761 actaatgtat tataaaagcc agttgacaca agtgttacac ctatgataga attaacttga
     2821 ggtgatgaaa atggcaaccg ataaaaatag aataatgatt agcttggatg ataaaaatct
     2881 agagaaactt gaaaatttag tagaagatgc tagagataga aggggaatgc gactaacaaa
     2941 gtcccaggtt attgaattac tcctaaatac cgttgattat ttcgatgata ttatgggggc
     3001 tatttactca aaaaaataag agcaaaattg ctattatttt ttacaaattg gtgtaacact
     3061 agtgatatag gagtgatgaa atggttgttt atattagaca aagtaaatta ccaagtgagg
     3121 tttcaattaa taaatacaat gctcaagtag gtgcctactt acaaggagaa gaagttattt
     3181 tatatcaatc tttttctgaa ataaaagagt taacaagtga agatatagtt gtagattata
     3241 taatggagac tagagcatta ctaaaaatga tgggcttaaa cgttccggtt catgactatc
     3301 ctattgagct taaagagttt tatggtcgaa agatttatgc tggtatttta ggagagattg
     3361 tgaatatacc tgataattgg ggaaaattta ttaagcctaa agctggttca aaagtcttta
     3421 ctggaagagt tgttaatgga acccatgatt taataggtat tggtctacct ttcgactatc
     3481 ctatatggat tagtgaggtt gtagaattca tagctgaatg gcgttgtttt gtgttagatg
     3541 gtcgcgtatt agatgttcga ccctatacag gtgattatca tgcacaattt gatgcaagtg
     3601 taattgatga agcgatatca tgttggaaag atgcgccaat agcttatgga ctagatatcg
     3661 gtgttactcg cgatggcaga acacttgttg ttgaagtaaa tgatggttat gcattgggaa
     3721 attatggcct atctccttta aaatcaatca attttcatag agctaggtgg aaagaaatgg
     3781 taaaacccta ttttgaaaaa aatgaaattt ttaaaatcca acaggatgtt attttctaaa
     3841 ataaatatgg tagataacca atataaaaag catcaaaatg aacaaggagg aatctgatgc
     3901 ttggagcaat tattggagat attgtcggtt ctgtttacga atggaacaat atcaaaacaa
     3961 aggactttcc tttatttcgg aaggactgct tttttacaga tgacacggtt atgacctgtg
     4021 ctgttgcaga agcaattatg aatggtggac aaaaagacga cttcattgac gcgatgaaga
     4081 aatatggcag aatgtatccg aatgctgatt acggtgctcg gtttaatgca tggctaaaca
     4141 gcgataaccg tgagccttat aatagctttg ggaatggatc agctatgcgt atttctccat
     4201 gtgcttggat catggactgt ggtttttatg cgaaaactgg tatatggcca tcatctagag
     4261 gacttacgag tctttctgca gaggtaactc ataatcatcc agaaggtgtc aaaggcgcta
     4321 tggctacagc tgatgctatc tttctatgtc gtttttactt tggtggttat tgtagagagt
     4381 acgagcaatc aattaacgac aatcctacag agtgtaaaag acgaattaag gattatatag
     4441 aaaaagaata cgattacaat ctatctcaaa ctttagatga aatccgtcct aactatcgtt
     4501 ttaacgaaac atgtcaggaa actgtccctc aagccattat cgcctttctt gaaagtagag
     4561 acttcgaaga tgcgataaga aatgccatct cacttggtgg cgacagtgat actctcgcag
     4621 caatcacttg tagcatagca gaggcagctt acggtattcc tgattggatc aaggataagg
     4681 cctattctta cttggatgaa cccttaaagg atgtagttag gcgatgggaa aatagaataa
     4741 aagcgtatta atctccagta cgtcagattt ctcttttctg tattttcatt gtataatagg
     4801 attacattta cttgcatacg acgaaatgta aacatttctg tttaaaatta aggataaaat
     4861 caatgaaaaa agcaatggta attatcaacc ctacttctgg tggcgagaag gctttggatt
     4921 acaaagaaaa gctggagaat aaagcaaaag aatactttga atatgttgaa accaaaatta
     4981 ccgaaaaagc gctggatgca acacattttg ctgaagaagc ttctcgtgag cagtatgatg
     5041 cagtggttgt gtttggtgga gatggaactg tcaatgaagt catttcaggt attgatgaga
     5101 gagactacat tcctaagtta gggattatcc caggcggtac gggtaacctc attacaaaac
     5161 ttttggaaat caatcaagac atcgatggcg caattgacga actggatttt gatttaacca
     5221 ataagattga tatcggtaaa gcaaatgaca actattttgg ttatatcttt agtatcggtt
     5281 ctctgcctga ggcgattcac aatgttgaaa tagaggacaa aacaaaattc ggtattttaa
     5341 cctatgctgt aaataccatg aagtctgtca tgacagatca ggtctttaac attaaggttg
     5401 agacagaaaa tggaaattat gttggtgaag ctagccatgt tttggtcctt ttgacaaatt
     5461 acttcgctga taagaaaatc tttgaagaaa acaaggacgg ctatgccaac attttgattc
     5521 tgaaagatgc ctctatattc tccaaattat ccgtcattcc tgatttatta aaaggggatg
     5581 ttgtcgcaaa tgataatatc gagtatatca aagcgcgtaa tattaaaatc tcttcagata
     5641 gtgaattgga gtcagatgtt gacggagata aatcagataa cctacctgta gaaatcaaag
     5701 tcctagctca gcgagtagaa gtattttcaa aaccgaaaga ggattagtat atagagaaag
     5761 ccttttttaa ggctttttgt atactttaaa agatagttcc tttaacaacg gacattcctt
     5821 gcaaatagtt tacaaaaata gtatactgga tttattgagt ttgaaaacgt ttgcgtaaaa
     5881 tttgaatgaa tactttagga gacaaattga tggaattgag tgctatttac cataggcctg
     5941 agtcggagta tgcctatctt tataaggata agaaactcca tattcgaatt cgaactaaga
     6001 aaggggacat tgaaagcatc aacttgcact atggggaccc ttttatcttt atggaggagt
     6061 tttatcagga tacaaaagaa atggtcaaga taacttctgg taccttattt gaccattggc
     6121 aggttgaagt gtcagttgac tttgcacgta tccagtatct ctttgagctc agagatacag
     6181 aaggtcaaaa tattttgtat ggcgataaag ggtgtgtgga aaattctcta gaaaatcttc
     6241 atgcaattgg gaatggattt aagttgcctt atcttcatga gattgatgcc tgcaaggttc
     6301 ctgactgggt ttcaaatacg gtatggtatc agatatttcc tgaaagattt gccaatggca
     6361 atgctctatt aaacccagaa gggactttag actgggattc atctgtcaca cctaagagcg
     6421 atgatttctt tggtggtgat ttacagggga ttattgatca tatggattac ttgcaagact
     6481 tgggtattac tggactatat ctttgtccca tctttgaatc tacaagcaat cacaagtaca
     6541 atacgacaga ttactttgaa attgaccgtc attttggaga caaggagacc tttcgggaac
     6601 tggtggatca agcgcatcat cgtggcatga aagtcatgct ggatgcggta tttaatcata
     6661 ttggttcgca atctcttcaa tggaaaaatg tcgtcaaaaa tggtgaacag tctgcttata
     6721 aggattggtt ccatattcaa caattcccag tgacaactga aaagctagtt aataagagag
     6781 acttacccta tcatgttttt ggtttcgagg actatatgcc taagctaaat acagccaatc
     6841 cagaggtcaa gaattatctt ttaaaggttg cgacttattg gattgaagag tttaatatcg
     6901 atgcttggcg tttggatgtg gctaatgaga ttgaccatca gttctggaag gattttcgta
     6961 aggcagtttt agctaaaaat cctgatcttt tcgagattat aaaaaatata aagaatctag
     7021 tcataaattt gaattgaaac aacgtcttta ttttttgctt cagcaatcta aatcgtttga
     7081 tgatttttta gagaaagcaa agcagttgca tgttcagatt gattttagtc aaaagcatag
     7141 ccgttttttg atgacagata gaacgatgat aaaaccaatt cgagggcgcc aactcagcaa
     7201 acgagatcta tatgatgaag aattttttag aacgcatttt gctaagcaag agattgaaag
     7261 tcgtttagaa tttttgttaa accgtgtcaa ttctttagaa gatttaataa caaaagcaaa
     7321 agaattgaat ctaaccattg acttaaaaca aaaaatgtaa cttttatcct ggaagaaaat
     7381 aatcaaaaga taagtttggg tcatcaaaaa ataagtgata agaaattata tgatgtcaaa
     7441 ttttttcaag attattttaa aaataaggaa gtcgttgctt cagaaggatt agagaattta
     7501 caggaacagt accatgcttt ccaagaagaa cgagataagg ataaagtatc cactgaagag
     7561 attgagaaag cctttaagac atttaaggaa aaatgagata ccgttcgtga atttgaagtg
     7621 gaacttgcag agaaccaaat agagaagcta gttgataagg gcgtctatat caaggtgtct
     7681 ttcggtgtca aacagagcgg tcttattttc attcccaact atcaattgga tattatggaa
     7741 gaagaaaatc ataaaaaata taaagtctac attcgagaaa cgtcttctta ctttgtctat
     7801 aataaagaaa acatggataa taattgtttc atcaagggta ggaccttaat cagacaattg
     7861 agcaacgata gccaaaaact cccctataga cgaccaactt taaaaagtct tcaagagaag
     7921 atttctgaaa ttaatcttat gattgaacta tctaacacga acaaacaata ccaagaaatc
     7981 aaagatgagc atgtattaga aattgcagag attgatatga agctagaaga aacccaagaa
     8041 aaaatcgcca ccttaaacaa gatggcggaa gtccttatca atttgaagag tgaagatcat
     8101 gagactagaa aattagctaa gtatgatttt gaccaaatga acatgacgga atcaataatg
     8161 ttagatcgac tcaatacaga tattttaaaa ttacaacagg aacttggtaa tgaaattaat
     8221 aaatatgagg agatagctag aagattggat ttgtttgtga aaattattaa taccaataaa
     8281 tttacagttc t
//
LOCUS       DAVKWS010000070         7831 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKWS010000070 DAVKWS010000000
VERSION     DAVKWS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.071"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            74..763
                     /gene="coaB"
                     /locus_tag="VW852_001859"
     CDS             74..763
                     /gene="coaB"
                     /locus_tag="VW852_001859"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV0272944.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            775..1326
                     /gene="coaC"
                     /locus_tag="VW852_001860"
     CDS             775..1326
                     /gene="coaC"
                     /locus_tag="VW852_001860"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV0272945.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1310..1873
                     /locus_tag="VW852_001861"
     CDS             1310..1873
                     /locus_tag="VW852_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0272946.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2156..2683
                     /locus_tag="VW852_001862"
     CDS             2156..2683
                     /locus_tag="VW852_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0272947.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2695..3210
                     /locus_tag="VW852_001863"
     CDS             2695..3210
                     /locus_tag="VW852_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV0272948.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3207..3674)
                     /locus_tag="VW852_001864"
     CDS             complement(3207..3674)
                     /locus_tag="VW852_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0272949.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(3748..4236)
                     /locus_tag="VW852_001865"
     CDS             complement(3748..4236)
                     /locus_tag="VW852_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272950.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(4202..4765)
                     /locus_tag="VW852_001866"
     CDS             complement(4202..4765)
                     /locus_tag="VW852_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0272951.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(4775..6763)
                     /gene="uvrB"
                     /locus_tag="VW852_001867"
     CDS             complement(4775..6763)
                     /gene="uvrB"
                     /locus_tag="VW852_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV0272952.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6840..7793)
                     /locus_tag="VW852_001868"
     CDS             complement(6840..7793)
                     /locus_tag="VW852_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0272953.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2341 a         1751 c         1377 g         2362 t
ORIGIN      
        1 gtaaatcttc gtttttacca aaaatttatc actttcattt tacttaccgc ttatttttgt
       61 gtacaatagt gctatgaaaa ttttagttac atcgggcggt accagtgaag ctatcgatag
      121 cgtccgctct atcactaacc attctacagg tcacttgggg aaaattatca cagagacttt
      181 gctttctgca gggcatgaag tttgtttaat tacgacaaaa cgagctctga agccagagcc
      241 tcatcctaac ctaagtattc gagaaattac caataccaag gaccttctaa tagaaatgca
      301 agaacgtgtt caggattatc aggtcttgat ccactcaatg gctgtttctg actacactcc
      361 tgtttatatg acagggcttg aggaagttca ggctagctcc aatctaaaag aatttttaag
      421 caagcagaat catcaggcca agatttcttc aactgatgag gttcaggttt tgttccttaa
      481 aaagacaccc aaaatcatat ccctagtcaa ggaatggaat cctactattc atctgattgg
      541 tttcaaactg ctggttgatg ttaccgaaga tcatctggtt gacattgcac gaaaaagtct
      601 tatcaagaat caagcagatt taatcatcgc gaatgacctg actcaaattt cagcaggtca
      661 gcaccgagct atatttgttg agaaaaatca gcttcaaaca gtccagacta aagaagaaat
      721 tgcagaactc ctccttgaaa aaattcaagc ctatcattct tagaaaggaa aactatggca
      781 aacattctct tggctgtaac gggttcaatc gcctcttata agtcggcaga tttagtcagt
      841 tctctaaaaa aacaaggcca tcaagtcact gtcttaatga ctcaggctgc tacagagttt
      901 atccaacctt tgacactaca ggtactctca cagaatcctg tccacttgga tgtcatgaag
      961 gaaccctatc ctgatcaggt caatcatatc gaacttggaa aaaaagcaga tttatttatc
     1021 gtggtacctg caactgctaa cactattgca aaactagctc acggatttgc ggacaacatg
     1081 gtaaccagta cagctctagc cctaccaagt catattccca aactaatagc tcctgctatg
     1141 aatacaaaaa tgtatgacca tccagtaact cagaataatc tgaaaacatt agaaacctac
     1201 ggctatcagc tgattgctcc taaggaatcc ctactagctt gtggagacca cggacgagga
     1261 gctttagctg acctcacaat tattttagaa agaataaagg aaactatcga tgaaaaaacg
     1321 ctctaatatt gcacccattg ctatcttttt tgctaccatg ctcgtgatac actttctgag
     1381 ctcacttatc tttaaccttt ttccatttcc aatcaaaccg accattgttc atattcctgt
     1441 cattattgcc agcattattt atggtccacg agttggggtt acacttggat ttttgatggg
     1501 attacttagc ttgacggtta acacgattac gattctaccg acaagctacc tcttctctcc
     1561 cttcgtacca aacggaaaca tctactcagc tatcattgcc attgtcccac gtattttgat
     1621 tggtttaact ccttacttag tctataaact gatgaaaaac aagactggtc tgattttagc
     1681 tggggctctt ggttccttga caaatactat ctttgtcctt ggaggaatct tcttcctatt
     1741 tggaaatgtt tataacggaa atatccaact tcttctggca accgttatct caacaaattc
     1801 aattgctgaa ttggtcattt ctgcaattct aaccctagcc attgttccac gactacaaac
     1861 cttgaaaaaa taaaaacaat ctccgctttc aaacctgaag gtggagattt tgctctatac
     1921 agtttctttt taatgaaatc tttactataa gtaaaacgaa ataagaacag gacaaattga
     1981 tcaggacagc caaatcgatt tctaacaatg ttttagaagt agaggtggac tattctagtt
     2041 tcaatctact ataactttta actaaaaaat gatctataaa cccaagtaaa ttcttgcttt
     2101 attgtctggt ttattgtacc atactagtgt atatgcagtt aaaaaggaga ttcttgtgaa
     2161 tacacggaaa aagacacaat ttatgacaat gacagccctt ttaacggcta ttgcgatttt
     2221 gattccaatt gttatgcctt tcaagattgt cattccacct gcttcctata ctttggggag
     2281 ccacatcgct atttttatag ccatgttctt gtcgcccttg atggcagttt ttgtcatcct
     2341 agcctctagt tttggatttt tgatggctgg ctatcccatg gttatcgttt ttcgggcttt
     2401 ttcccatata tcttttggtg ctttaggagc tctttaccta caaaaattcc ccgataccct
     2461 agataaacca aaatcttcct gggttttcaa ctttgttttg gctgttgttc atgcccttgc
     2521 tgaagtatta gcctgtgtcc ttttttacgc aacttcgggt accaatgtag aaaacatgtt
     2581 ctatgttcta tttgtgctag ttggatttgg cacaattatc catagtatgg tagactatac
     2641 attagcacta gctgtctata aagtgcttcg aaaacgccgt taaaaggaaa agctatgaca
     2701 aaagatcgca aacaagccct gctccaactc ttgaaagaag ctcctaaagc ccttaatggc
     2761 caaagtttgg cggaacattt tcatgtcaca cgtcaggtca ttgtgcagga cattgcaatt
     2821 ttgagagccg atggcgctcc tatcctatcc accaatcgtg gctacatcta taagcaaatt
     2881 gataccaatc cttatgttca caaacttttc aaagtgaaac atgaagttga agaaatcggt
     2941 caggaactcc ttgctattgt cgataatggt gggcatgtcc agaatacctt gattgaccat
     3001 cccgtttatg gagaaattga aaccttgctt aaactgtctt gtcgcagaga tgtgcaacat
     3061 tttctagaac aagtcgagca ttctgacttt agacctctat ctgaattgac agatggcatc
     3121 cattaccacc tagtcgaagc cgaaacacaa caagacctcc actatatcga ggaggccttg
     3181 gatcagctag gttatttagt aaaagactag aagattttct tttcccaatc gtcttctgta
     3241 cggcgaggat agaaaaactc tgacttgtcg ggagcttcca tcatttccat caatggtagc
     3301 atatcatagg ccagatttaa gtttggaatc tggtcttttt gcacccaaga aacttctccc
     3361 tcttctgaag attgaagggt accagagaac tcagtcgcct tataacaaat gacaatatag
     3421 cgcccacctg tatctagtgg ccaattttta atgccgacaa gttgaggatt ttggatagtc
     3481 aaccctgttt cttcgtagat ttcacgaatg acagactccg caaaagcctc atcattttct
     3541 acatgacctc caggaaaggc ataaccagac cagcgattgt tttcaggggc gcgatactgc
     3601 atcaccacgc gctgagtttc gaggtcttca atcagacaga tatttgttaa aattgttaat
     3661 tgggaacggg acataaattt actcgctttc taatttatta aattttcaga cttactacca
     3721 gagatattat cttttacttt tttaaaatta tttagaatag aacaccattc gtttttcctt
     3781 tataggcttt ccaagttcaa gtattttttc ttgtccatca aaagtaaaac ccattttttg
     3841 atagaaagca atggcgcgct tgttatcttt caatacccat aagaaaattt cagaaaaatg
     3901 attaagagca gtcaaagctg ctttcattaa cttttgtgcg ataccttttc cataatagtc
     3961 ttttaaaaca tataaagcaa taatttcacc agcttgaata gtctcatcac gaaaattgcc
     4021 ataactgata aaaccaacca ccttcacgcc atccatcgca atcaatgtat tttctggata
     4081 cttttgacta aagaatcgac atctttctaa tgtcattgta tcctgaaatt ccgcaggcaa
     4141 aaggtcatca taagactctc tccacgtttg ccagtgaacg agggatttgc cttctatctc
     4201 ttcaggagtt tccattgatt tgataataac cgtcatttat tttctcccag tcttctctca
     4261 aaataccata tttaatacta tcaaaatatt taccttgata ataacgaact tttggaatat
     4321 gagcttcttt tttcattctt aatttttcag caagtttcat cataccaaga tttcctgacc
     4381 aagttgtcaa acccagatgc tccaactcca agtaatcctg aaacgtccta tctatccact
     4441 gcaacatagc aactttccca ataccagtgt tccagaattt tttatcataa ataccaattc
     4501 ccaattccat ccatcttgtt tctttacata cccaataacg cgaaacagtc ccaactagtt
     4561 tatcatcaac aaaaatacca aggcgatttg agttgcttaa aatggattct gatttttgta
     4621 gttcgaattc tttaaaattt gaaaaatact gataatcatc ataataggga gcatcatact
     4681 gtttccaaac tggattaagc tgtgaataag ccatttccca taaagacatc aaatcttctt
     4741 tctttaactt tcttaaataa atcatactat tcccctaatc caaggccttg acttccaaca
     4801 tcatatcacg gatctgagcc gccagttcaa agtcaagcac ttcgacggct tcttgcattt
     4861 gtttttctag tttcttgacg agttctttgc gctcttgttt gttgaggcta ttgatgtcga
     4921 cttccttgtc ctcttcctta gcaactgcct tggtcacggc aatcaggtca cggatttctt
     4981 tcttgattgt ctgtggtacg ataccatgct cttcattata ggccatctga attttccgac
     5041 ggcgggcagt ttcatcgata gcacgttgca tagactgggt aaccgtatct gcatacatga
     5101 tgacatgacc ttcactgtta cgggcggcac gcccaatggt ctggatgagt ccacgctcgt
     5161 tacgaaggaa accttccttg tcagcatcga gaatggcaac caagctaacc tcaggaacgt
     5221 caatcccttc acgaaggaga ttgattccaa ccaagacatc aaagacaccc aagcggagat
     5281 cacggataat ctccgtccgt tccaaggtct tgatatccga gtgcatgtac ttgaccttga
     5341 tgcccatttc cttgaaatag tcggtcaagt cctctgccat tttcttggtc aaggtggtga
     5401 taaaggttcg ttcattcttt tcaacacggg cattgatttc acctaagaga tcatcaatct
     5461 gtcccatagt cggacggact tccacctcag gatccaaaag ccctgtcggt cgaatgattt
     5521 gctcaatcac cgtctcagtc tgttcatttt catagtcacc aggtgtcgct gatacgtaaa
     5581 caatctgatg cacatggctt tcaaactcct cacgacggag aggacgattg tccaaggcag
     5641 acggtaaacg gaaaccataa tttactagca tttccttacg cgaacggtct ccattgtaca
     5701 tgccttttat ttgtcccata gtcatatgac tctcgtcaat cataatcaaa aaatcatctg
     5761 ggaaaaagtc gagaagcgta taaggaggct caccttcact acgtccatcc atgtggcgag
     5821 aataattttc aaccccattt gtatagccca tctcgcgcag catttcgata tcgtactctg
     5881 tccgttgttt caagcgctgg gcttcaagca atttaccttc cttctcaaag acagctaact
     5941 gctcttctaa ttctgcttga atcttggcaa cggcaacttc catatggtcg tcattggtca
     6001 caaagtgagt agctgggaaa atcgccaaat gatccacctc tcccaatacc tgacctgtca
     6061 gagcctcaac ctcacggata cggtcaattt cgtctccaaa aaattctact cgaaaggcat
     6121 gttcatctcg ggaagctggg aaaatctcta ccacatcccc acgaacgcga aatcttccgc
     6181 gttggaaatc aatatcatta cgttcaaact gaatatcgac caagtcattc aagagtttat
     6241 cacgagaaat ctctagacca ggacggagac taacgacact atcagcgtat tccttgggcg
     6301 aacccaaacc atagatacaa gagactgagg ccacgacaat aacatcatta cgctccaaaa
     6361 gggctgaggt agctgagtgg cgaagcttgt caatctcgtc attgacagaa ctatccttct
     6421 caatataggt atcgctagaa gggacatagg cctctggctg gtaataatca tagtaggata
     6481 cgaaatactc aactgcattt tcagggaaaa attccttaaa ctccccatag agctgaccag
     6541 ccagagtttt attgtgggca ataaccagag ttggtttatt gactttagaa atgacctgac
     6601 tcatagtata ggtcttccct gttccagtcg cccccatcag aatctgagct ttttctcccc
     6661 cctcaatgtt atccaccaac tgctcgatag cttggggttg atctcctgat ggttgatatt
     6721 ttgatactag tttaaattga ttatctgtaa tgtgattgat cataagaatc ctctctccat
     6781 gtgctattcc tattttagca tacttatagt attttcacga agaaaaggac ggaccgaagt
     6841 cacatccttt cattttcttt ctttaattta ttagcaagat agacaatcag cagtagtaat
     6901 accagacttg tcataatgga accttctatg ccaaaagaac cacctgatag ccaatcttga
     6961 ttgccttgtg gtaaaaaggt catcagagac gttcctgacg gttgaccact aactaaaatc
     7021 ccaaagagat taccctgagc aaaattccaa gcaccatgaa tacctgcaac accccaaact
     7081 gtatcagttt tgagaagata aagagccatg gcaactccga ataaaaagag atttactaga
     7141 gatagagggg tgaggccaga attgcccata tgaagcaggg taaagaacag gctagatata
     7201 agaatagcta gttttagatt ggttcttgag gccaattgag gaagtagcca agcacgggcc
     7261 accacttctt ctgctgtccc ctgtaaaatc caaaatggga tagtaaagac gacaaaggca
     7321 agagaataag gattcaagtg aatggattcc aaacgatatt gacctaagac cactaaacct
     7381 aacaaggtca gaagaaaaag tgccaagcct agactaaatc ctttcagaag attgctgagg
     7441 aaattctctc tataaaatcc caaggttcga ataggtcttt tctcaacttt tcttgtccat
     7501 ctgaacacag tgttaagaat aaaaccaaag gccagtaatc ctaaaattaa acgaaagtca
     7561 cttgttttat ccgtcaagct ctgatgcaag gtctgcaagt aggttgcaag attttgacca
     7621 gcctctccaa aatttctagc aagtccgatg agagctaaca agccaatacc atacaaagta
     7681 aaagccacaa actctcctat gaagacaaaa acaaaggcta acaaggggct tgtgtaaatg
     7741 tttaaactca tttttgaagc ttggaagtct ttaaatattc ttttgttctt catatccccg
     7801 tcctcttttc ttctatatat actattatta t
//
LOCUS       DAVKWS010000071         7652 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKWS010000071 DAVKWS010000000
VERSION     DAVKWS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.072"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..2235
                     /locus_tag="VW852_001869"
     CDS             <1..2235
                     /locus_tag="VW852_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV0272954.1"
                     /translation="TPNTLLHDYSNIVDNVLTDLNSVQYDSNAIKKILDISDKVKNTE
                     LYLDEQFVKTKANIKDTLSKLLSADAAIAENSNSIIDNYVIQKIKQNKEALLLGLTYL
                     ERWYNFKYGETKAKDLVMYHLDFFGKSNSSALDNVIQLGKSGFNNLLAKNNVITYNVL
                     LSKNYGTESLFKALEGYRKVFLPTTSNNEWFKAQTKAYIVEEKSSIPEVSKKQSEQGT
                     KYSIGIYDRLTSPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDRYRSKVHPKG
                     DNLNKFVEDNVREAAKRFRDHYDYWYKILEPENREKLYRSLLVYDAFKFGRDNTEDKV
                     TYQADFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKDGAVTYTHEM
                     THNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYDDSENSTRLQ
                     VADPTQRFNSAEDLHNYMHNMFDLIYTLEILEGRAVAKLDYNAKNDLLRKIENKYKQD
                     PDGNSVYATNVVRRLTMDEVNKLNSFDSLIENDIITSRGYKDQEYKRNGYYTIDLFSP
                     IYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYISNQYEKDAKAAGSKIKSYGK
                     EVGLVTDKLVLEKVFNNRYSSWVEFKKAMYNERIAKFNNLISVSFNNPNVSWGRTNRI
                     TITSIANLQNMINTAVNEDAEDFRAQLYPDTNSRVLKLKKAIYKAYLDQTNDFRRSIF
                     ENKK"
     gene            2279..7516
                     /locus_tag="VW852_001870"
     CDS             2279..7516
                     /locus_tag="VW852_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0272955.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSIFLGSFLFALSVVGVSTYHYLDYS
                     TLTQTERDQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTVLGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDR
                     TVSSVSPASTVSPVEQPKVVTDKGEPEVQPALPEAVVTEKGEPEVQPALPEAVVTEKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDNSVKQVSEDGERQIVT
                     SYEELHGKKISESVETVTILKEMKPEIIVKGTKEKTAPVLTLTKVTEDAMNRSANLNY
                     ELDNKDNAEISSIVAEIKDGDTVVKRVDLSKEKLTDAVQNLDWFKDYKIATTMIYDRG
                     QGSETSKLDERPLRLELKKVEIKNIASTNLVKVNDDGTETPSDFMNEKPSEEDVKKMY
                     LKITSRDNKVTRLTVDSIEEVTEEGQKLYKITAEAQDLIQHTDPTKVRNKYVYYIEKP
                     HPKEDNVYYNFKDLVDAMNTDKNGTFKLGADLNATGVPTPKKWYVDGDFRGTLKSVEG
                     KHYTIHNTERPLFQNIIGGTVTKVNLGNVNINMPWADRIAPIADTIKGGAKIEDVKVT
                     GNVLGRNWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYV
                     DANLIGKKAKAAGIAYWTQNSGDNHKVGVEGAVKKGIVKGTIQVESPVEVGGAVGRLS
                     HHGYIGEVVSMMKVKKGEIFYGSSDMNDDPYWVANNVRGNYVVNGVSEGTISYARAKE
                     HHRIKPISQSEADTKIMMLGITAQDFAINEPVVNRLNRLTRKEDEYKSTQDYKVDRDL
                     AYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMV
                     HYADGTKEEMDVTKNADSKVKQVREYTIAGQNVVYTPNMVEKDRNQLIQDIKDKLASV
                     QLISPEVRALMDARKKPEENTDERKNGYIKDLYLEESFAETKANLDKLVKSLVENADH
                     QLNSDETAMKALVKKVDENKAKIMMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSV
                     DLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDW
                     FIHAAKNVYVSEPQTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYN
                     TMAYSSFEKYGKYTEEARNEFKKEIDKVAHAQQTYLDFWSRLALPSVRDQLLKSENRV
                     PTPVWDNQNYSGIKGINRMGYDEKKVPIAPIRELYGPTWKFHNTNWNMGAMASIFPNP
                     NNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTP
                     SSIGHQGEYGALGLNMAYHRENDGDQWYNYDPDKLQTREDIDRYMKNYNEALMMLDHV
                     EADAVLPQLNGDNSKWFKKIDREMRRNLGDGLNNLVAPHQWDNVRDLNQEESSKKLSS
                     INDLIDNNFMTKHGNPGNGRYRPEDFRPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKH
                     NAFRMWGYYGYDKGFTSYVSSKYQGEADKQNQGRLGDDFIIKKVSNNQFSNLEDWKKY
                     WYHDVKSRAEKGFTEITIDGQTIHNYNELKDLFDKAVTEDLKKAGNYSNTENLKSKVF
                     KALLKNTDGFFNPLFKKDI"
BASE COUNT         2787 a         1123 c         1516 g         2226 t
ORIGIN      
        1 actcccaata ctctattgca tgactacagt aatattgtag ataatgtttt aactgacttg
       61 aattctgtac aatatgattc aaatgcaatt aagaaaatat tagatatctc agataaagtt
      121 aaaaatacgg aattatactt agatgagcaa tttgttaaaa caaaagcaaa tataaaagat
      181 actttatcaa aacttctgtc agcggatgca gcaatcgctg agaatagtaa ttcgataatt
      241 gataattatg ttattcagaa aatcaaacag aataaagaag ctttattgtt aggattaaca
      301 tacttagagc gttggtacaa ttttaaatat ggtgaaacga aagcaaaaga tttagttatg
      361 tatcacttgg atttcttcgg taaatctaat agttctgcat tagataatgt tattcaatta
      421 ggtaaatctg gatttaacaa tttactagca aaaaataatg taattactta caatgtatta
      481 ttatctaaaa attatggaac tgaaagttta tttaaagcat tagaaggata tagaaaagta
      541 ttcttaccaa caacttctaa caatgagtgg tttaaagctc aaactaaagc atatattgtt
      601 gaagaaaaat cttctattcc agaagtaagt aaaaaacaat cagaacaagg aactaagtat
      661 tctataggaa tttatgatag attaactagt ccgtcatgga aatatcaaag tatggtctta
      721 ccactattaa ctttacctga agaaaaaaca gtatttatga tagcgaatat ttcaacaata
      781 ggatttggtg cttatgatag atatagaagt aaagtccatc ctaaaggaga taacttaaat
      841 aaatttgtag aagacaatgt aagagaagct gcaaaaagat ttagagatca ttatgattat
      901 tggtataaaa tattagagcc tgagaataga gagaaacttt atagaagttt attagtatac
      961 gatgcattta aatttggtcg agataatact gaagataaag taacttatca agctgatttt
     1021 gaaactgatc atccagctat aaaatacttc tttggacctg cggggaataa tgttgttcat
     1081 aatggacacg gagcatatgc taccggagat gcattctatt atatggcata tcgtatgttg
     1141 gataaagatg gagctgtaac atatactcat gaaatgacac ataactctga tagagaaatt
     1201 tatcttggag gatatggaag aagaagcgga ttagggcctg agttttatgc taagggctta
     1261 ttacaagcac ctgaccaccc atatgatcca acaataacaa ttaactctgt gttgaaatat
     1321 gatgattcag aaaattcaac aagacttcaa gtagctgatc caactcaacg atttaatagt
     1381 gcagaagacc tacataatta tatgcataat atgtttgatt taatatatac tcttgaaata
     1441 ttagaaggtc gtgctgtagc taaattagat tataatgcga aaaatgattt attaagaaaa
     1501 attgagaata aatataagca agatccagat ggtaattctg tttatgcgac aaatgttgtt
     1561 cgcagattaa cgatggatga agttaataaa ttaaattctt ttgatagttt aattgaaaat
     1621 gatattatta ctagcagagg gtacaaagac caagaatata agagaaatgg ttattataca
     1681 attgatttat tttcaccgat ttattcagct cttagtggag agaaagggac tcctggtgat
     1741 ttaatgggac gtcgtatagc attcgaatta ttagctgcaa aaggatataa agagggcatg
     1801 gtaccttata tctcaaatca atatgaaaaa gatgcaaaag ctgcgggaag taagattaaa
     1861 tcttatggta aagaagtagg tttagttaca gataaattag tacttgaaaa ggtatttaat
     1921 aatcgttact catcttgggt agaatttaaa aaagcaatgt ataatgagcg aatagctaaa
     1981 tttaataatc taataagtgt tagctttaat aatccaaatg taagttgggg aagaactaat
     2041 agaataacaa taactagcat agccaacctt caaaatatga taaatacggc tgtcaatgaa
     2101 gatgctgaag attttagagc acaattatac cctgatacaa atagtagagt tcttaaactg
     2161 aaaaaagcaa tatataaagc ctatcttgac caaacaaatg attttagaag atcaattttt
     2221 gagaataaaa aatagtgttt actattagga aataaaaagt gaatgtggag gattagaatt
     2281 gtcattatta aaaaaagata aattttccat taggaaaata aagggtattg ttggttcgat
     2341 attcttgggg agttttttat ttgcactgtc agttgttggt gtatcgactt atcattactt
     2401 agattatagt actttgacac aaactgaacg tgatcaactc aaacaaggta gacctgatga
     2461 atcaaaagag tcatatgctt tggtttatga aaaagacgca ttaccaaaca caggaagttc
     2521 tcaatctatt atgactgtat tgggtttgtt agctatcggg agtcttattg tgattatcac
     2581 aaaagataaa aagaggaaaa aaatagctac atttttgatt gtaggggcga caggtttagt
     2641 tacactatca actgcttcag cacttaattt gaatgctaac atccatgagt ctggacgtga
     2701 tggtgtgttg caaatttctg gttacagata tgttggttac ttggagcttg atgatagaac
     2761 tgtttcgtcg gtttctccag catcaactgt ttctccagtt gagcaaccca aagttgtgac
     2821 cgacaaaggc gaaccagaag ttcagccagc gttaccagaa gcagttgtaa ctgaaaaagg
     2881 ggaaccagaa gtacaaccag ccttacccga agcagttgta actgaaaaag gggaaccaga
     2941 agttcatgag aagcctgact atacgcaacc aataggtgca aaccttgttg agcctgaagt
     3001 tcatgagaaa ctagcataca ctgaacctgt aggtacaaca ggagtagatg aaaatggaaa
     3061 cttgattgag ccacctgtta acgatattcc agagtatact gaaccaatat ctacagtttc
     3121 agaagttgca tcagaaagag aagagttgcc ttctttacat accgatattc gtacagagac
     3181 tattcctaaa accactatag aagaatctga tcctagcaaa tttataggcg ataattctgt
     3241 taaacaagtt agtgaggatg gcgaacgtca aattgttact agctatgaag agttacatgg
     3301 caaaaaaatc agcgagtcag ttgaaacagt aacaattttg aaagaaatga agcctgaaat
     3361 tattgtaaaa ggtacaaaag aaaaaacggc tcctgtttta accttgacca aagtaacaga
     3421 agatgctatg aatcgttctg ccaacttaaa ttatgagctt gataataaag ataatgctga
     3481 aatatcaagt attgtagctg aaattaaaga tggtgataca gtagttaaaa gggttgattt
     3541 atctaaagaa aaattaactg acgctgtaca aaatttggat tggtttaaag attataagat
     3601 tgcaacaaca atgatttatg atcgtggtca aggttctgaa acgtctaaac tggatgaaag
     3661 acctctgcgt ttggagttga aaaaggtcga aattaaaaat atcgcatcta ctaatttagt
     3721 gaaggtaaat gatgatggta ctgaaacacc tagtgatttt atgaatgaaa aaccttcaga
     3781 agaagatgtt aaaaaaatgt acttaaaaat cactagtcgc gataataagg taactcgttt
     3841 aactgtagac tctattgaag aagtgactga agaaggtcaa aaactttaca aaataactgc
     3901 tgaagcacaa gatttgattc aacacacaga cccaacgaag gttcgtaata agtatgttta
     3961 ttatatagaa aaaccccatc ctaaagaaga taatgtctac tataacttta aagatttagt
     4021 agatgctatg aatactgata aaaatggtac atttaaactt ggtgcagatt tgaatgccac
     4081 tggtgttcca acacctaaga aatggtatgt tgacggcgac tttagaggta cattgaagag
     4141 tgtagagggg aaacattata ctatccacaa cacggagcgt ccgttattcc agaatattat
     4201 tggtggtaca gtaaccaaag taaatctggg aaatgtaaat atcaatatgc catgggcaga
     4261 ccgcatcgca ccgattgctg acactattaa aggtggcgct aaaattgaag atgtcaaagt
     4321 gactggtaac gtattaggta gaaactgggt atctggattt attgataaga ttgacaacca
     4381 aggtacactc agaaatgtag cctttatcgg aaatgtgaca tctgtaggtg atggtggtca
     4441 attcttgacc ggtatcgttg gtgagaactg gaaaggacta gtcgaacgtg cgtatgttga
     4501 cgcaaatctt attggcaaga aagccaaggc tgctggtatt gcttattgga cacaaaacag
     4561 tggtgacaat cataaagttg gtgtcgaagg tgcagtcaag aaaggaattg tgaagggtac
     4621 cattcaggta gaaagtcctg ttgaagtcgg cggtgctgtt ggacgtttga gtcatcatgg
     4681 ttatatcggg gaagtcgtat ctatgatgaa agttaagaag ggtgaaattt tttatggttc
     4741 tagtgatatg aatgatgacc catattgggt ggcgaataat gtacgaggaa attatgtcgt
     4801 taatggtgtg agtgaaggta cgatatctta tgctcgtgct aaagaacacc atcgtatcaa
     4861 accaatttca cagtcagaag cagatactaa aattatgatg ttaggtatta cagcacaaga
     4921 ttttgctatc aacgaacctg tggtcaatcg cttaaaccgt ttaaccagaa aagaagatga
     4981 atataagtca acccaagact ataaagtaga tcgagatctg gcttatcgta atattgagaa
     5041 acttcaacca ttttataaca aagaatggat tgtcaaccaa ggtaacaaac ttgcagaaga
     5101 ttcaaactta gctaagaaag aagtcctttc tgttacgggt atgaaggatg gacagtttgt
     5161 aactgactta tcagatattg ataagattat ggttcactat gctgatggta ctaaagaaga
     5221 aatggacgtc actaagaatg ctgattctaa agtgaaacaa gttcgtgaat ataccatcgc
     5281 tggacaaaac gttgtttata caccaaatat ggttgagaaa gaccgtaacc aattgattca
     5341 agatatcaaa gataagttgg cgtcagttca attgatttct cctgaagtac gtgctttgat
     5401 ggatgcacgt aagaaaccag aagaaaatac tgatgaacgt aagaatggtt atattaaaga
     5461 cctttactta gaagaaagct ttgctgaaac gaaagctaat cttgataaat tggttaaatc
     5521 cttagttgaa aatgcagatc atcaattaaa tagtgacgaa acggctatga aagcacttgt
     5581 taagaaggtg gatgaaaata aagctaaaat catgatggct ctgacttatc tcaatcgtta
     5641 ctatgatatc aagtatgggg atatgactat caagaacttg atgatgttta aacctgattt
     5701 ctatggtaaa tcagttgacc ttcttgattt cttgattcga attggatcaa gtgagagaaa
     5761 tatcaaaggt gacagaactt tggatgctta tcgtgatatg attggtggaa ctattggtaa
     5821 gtctgaatta catggattct tagattacaa tatgcgtctc ttcactaacg atactgattt
     5881 aaatgattgg ttcatccatg cagctaagaa tgtatatgta tcagaacctc aaacaacgaa
     5941 cccagatttt gttaataaac gacatcgtgc atttgatggt ttgaacaacg gtgttcataa
     6001 tcgtatgatt ctacctctat taactcttaa gaatgcacat atgttcttga tttcaactta
     6061 caatacaatg gcttatagtt cttttgagaa atatggtaag tatacagaag aagctagaaa
     6121 tgaattcaaa aaagaaattg ataaggttgc acatgctcaa caaacatact tagacttctg
     6181 gtcacgttta gcattgccaa gcgtacgtga ccaattactc aagagtgaga atagggtacc
     6241 aactcctgtg tgggataacc aaaactatag tggtattaaa ggaataaacc gtatgggtta
     6301 tgatgagaag aaagtaccaa ttgcgccaat tcgtgagtta tatggtccaa cttggaaatt
     6361 ccataatacc aactggaata tgggagctat ggcatctatt ttcccaaatc ctaataacaa
     6421 tgaccaagtt tatttcatgg gaacaaatat gattagtccg tttggtattt cagcctttac
     6481 tcatgaaact acacacgtta atgatagaat gctttacttc ggtggtcata gacaccgtca
     6541 aggtacagac gtcgaagcct atgctcaagg tatgctacaa actccgtcaa gtattggtca
     6601 ccaaggtgaa tatggtgctt taggtttgaa catggcttat catcgtgaaa atgatggtga
     6661 ccaatggtat aactatgacc ctgataagct tcaaactcgt gaagatatcg atcgatatat
     6721 gaaaaactat aatgaagcac tgatgatgct agatcatgta gaagcagacg ccgtacttcc
     6781 acaattgaat ggtgataata gcaagtggtt caagaaaatt gatagagaaa tgcgcagaaa
     6841 tcttggggat ggtttgaata acttagtagc accgcaccaa tgggacaacg ttcgtgactt
     6901 aaatcaagaa gagagttcta agaaattatc aagtattaac gatttgattg ataataattt
     6961 catgaccaaa cacggaaatc caggtaacgg acgttataga ccagaggatt ttagaccaaa
     7021 ctcagcttac gtaaatgtca atatgatggc aggaatttac ggtgggaata cgagtcaagg
     7081 tgctcctggt tcattatcgt tcaaacataa tgccttccgt atgtggggtt attatggata
     7141 tgacaaaggt ttcactagtt atgtatctag caaatatcaa ggtgaagctg ataaacaaaa
     7201 ccagggtaga ttaggtgatg atttcattat caaaaaagtt tcaaacaacc aattctcaaa
     7261 tcttgaagat tggaagaaat attggtatca tgatgtgaaa tccagagctg aaaaaggatt
     7321 cacagagatt acaattgatg gacaaaccat tcacaactat aatgaactga aagacttatt
     7381 cgacaaagct gtcacagaag acctcaagaa agctggaaac tactcaaata ctgagaattt
     7441 gaaatccaaa gtcttcaaag ctcttcttaa aaacacagac ggtttcttta atccattgtt
     7501 caaaaaagac atttaaaccc caattattca ccccaaatct aaaaaccatc cagaatcctt
     7561 gccttagctt agatcctgga tggtttcttt ttcacccaat gggtgttttt tactagacaa
     7621 aaaagagttt cccctttatg gtataagtgt ag
//
LOCUS       DAVKWS010000072         7632 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKWS010000072 DAVKWS010000000
VERSION     DAVKWS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.073"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            99..509
                     /locus_tag="VW852_001871"
     CDS             99..509
                     /locus_tag="VW852_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HEV0272956.1"
                     /translation="MEAIYQRDSDQDGLTDAQELALGTNPLSSDSDGDGRSDLVEIEE
                     GTNPLEKDLQDIDQTSITESSSVFMEMKQKISDMMESHYKEFILALISIETGIENQQD
                     LEDLYTYYMRMDAVSLLSSDLETSPQEVEMEIEL"
     gene            557..6781
                     /locus_tag="VW852_001872"
     CDS             557..6781
                     /locus_tag="VW852_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0272957.1"
                     /translation="MEVIQLLAMFRGTIPKDRDKMDLFLRYQAQHFDEKWQDLVESFL
                     TEEEKIEEIPHVYSFHQDIVSFLEASSENNDQDLESYTRNFGQAGLDKLSQLSNFEKN
                     LVLKVASYNLSTRFYIQSEKEKLTPLSELVFHQNQDVNLVNVYRVANNLSDRISRDIE
                     EFLLMVDSKEVKKEVPEIHFEEKEGDVLAYLGSELMATLDIVTDLVHHEENYIQLPLT
                     QKLKIITHFDEVKAKSEKSNQVEKILSPSSDIEQETEETNSFSNVDKIVEEALREYPI
                     GSQVSYKGQVFQLVSIENAQLNDLVRLELFNDSNQLFEENPILYLNSLEEIEQVLSHL
                     ELEKEDSEIEIDSSSESQEIDLFSYLEEENVNEKDKDKETKSLISGIEETDVPVLDFV
                     FPDDLEDFYPKTNREKIETNIAAIELVKRLEKERRQANPEEQELLAKYVGWGGLANEF
                     FDELNPKYETERLTLKSLVSKSEYSTIKQSSLTAYYTDPMIIRQIWQKLLDDGFEGGR
                     ILDPSMGTGNFFAAMPRSIREKSELYGVELDSVTGAIAKQLHPNTHIEVRGFEEVPYQ
                     NNSFDLVLTNVPFGNFRIADKNYDKPYMIHDYFVKHSLDLVRDGGQVSIISSIGTMDK
                     RTDNVLQEIKSNTHFLGGVRLPDTAFKKIAGTRVTTDLLFFQKDQAKNLNEEELVFSG
                     SVPFEEDKRVWINPYFDGKYNTQVLGEYEVRNFNGGTLNVKGESETLATDIMKALENV
                     EAPKQIDNSLKAPVFIQEEVDNSIPSHIRENLALYSFGYEGNQIYYRDTHGIRRSSKV
                     DEISYYVDEKGDFKAWDSSLSEHKIDRFVQLHLTDEEALDVYKSEEASKRGKYKGLFK
                     KTVFYESPLSDKDISRIKGMVDLRETYQALIEIQRHPDYSRTDFQVLLSKLNRDYDRF
                     VSQFGYLNVSVNRNLFDSDDKYSLLASLEDEYIDSKDQKVKYKKSLAFEKALVRPERV
                     ITRVSTALDALNSSLSDGRGVDIDFMVSIYPEHSQAAILDELGDQILMDPESYLRGER
                     NYLSKNQFLSGDILNKIEVVQLLVEENNQECDWTHALDLLESVRPPRIHLADIEFKIG
                     SRWIPQSVYGKFAFECFTNREFELSSPDVEQVIEVNPVDGQVHLRTPFAYRYPSAKDS
                     SLGVSGSRYDTGRKIFENLLNSNQPTITMTVTEGEKKKTITDLEKTSVLRAKEQHLQE
                     LFQDFVSRYPEVQQVIEESYNRLYNRTVSREYDGSHLVIDGLAQNISLRPHQENAIQR
                     IVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLSAQFGQEIMKFFP
                     TKKVFVTTKKDFVKARRKQFVSRIITGDYDAIVIGDSQFEKIPVSKERQMNYIEDKLN
                     ELREIKTHSENKYTVKEAEQSISGLEKQLEELQRFNRDSFIDFENLGIDFLFVDEAHH
                     FKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQIQEEHDFKNIVFATGTPVSNSISEL
                     YTMMNYIQPDILKRYQVDYFDSWVGAFGEIQNSMELAPTGDKYQPKKRFKKFVNLPEL
                     MKIYKETADIQTQDMLDLPVPEAHIIPIESELTENQKLYLEELVMRSDAVKCGTVDPS
                     QDNMLKITGEARKLAIDMRLLDSSYSLADNHKLLQVVDNVERIYREGMENKATQMIFS
                     DIGTPKKKDNGFDVYSEIKALLVDRGIPSMEIAFVHDANSDEKKNSLSRKVNAGEVRV
                     LLASTEKGGTGLNVQSKMKAVHHLDVPWRPSDIQQRNGRIIRQGNENKEVDIYHYITK
                     GSFDNYLWATQENKLRYIKQIMTSKEPIRAAEDIDEQTMTASDFKALATGNPYLKYKM
                     ELENDLTLLENQRRAFQRSKDHYRHTISYCEENMPILEKRLSKYEGDIQQSEMSKDQS
                     FSMTIGKQVFEQRAEAGESLHRLIRHNQSDSKEFRTLASYRGFDIKMLSLATNQPLPE
                     TFSVKIVGENQYSVSLDLYSPLGTIQRLQHTIDHIKDDQVKTQNLLDELKDKWTTAKV
                     EIEKNFPKEEDYQTKKAEYDVLAPLIETETDLDIIDQALRQFHEKGKEKQEQLSFELD
                     "
     gene            6856..7140
                     /locus_tag="VW852_001873"
     CDS             6856..7140
                     /locus_tag="VW852_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272958.1"
                     /translation="MMEDTYYQLEEALVQGFQTPEEYQAYKELKEHYEEVTGDYSFSK
                     RELTSQLEIALQNHQGVDFEEHEKEEYLDLVQKLEEFDSSLATHYRQLID"
     gene            7154..7450
                     /locus_tag="VW852_001874"
     CDS             7154..7450
                     /locus_tag="VW852_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272959.1"
                     /translation="MNDLLLIPVIFLAVGGIIILLWRLFLIASGLFLIGFVSFLIFVE
                     VYGIYLFFTEPSLYFDDIRQYGLTSFTAVYLFINLMLVLGFSWRFINSINKHKM"
BASE COUNT         2633 a         1173 c         1593 g         2233 t
ORIGIN      
        1 agttttggct cgttctcttt ttttgttgtg atacttcttt caaggaatat ttggtaggat
       61 tggagtgaaa aaaaattaac taggaaagaa ggaagtatat ggaagccatt tatcaacgtg
      121 attcggatca agatggatta actgatgctc aagaattggc cctaggaacc aatcctctta
      181 gctcagattc tgatggtgat ggacgttcag atttagtgga aatagaagaa ggaaccaatc
      241 ccttagaaaa ggatttacaa gacatagacc aaacaagtat cactgaatcg tcatcagtat
      301 ttatggaaat gaaacaaaag atttcagata tgatggagag tcactacaag gaatttatac
      361 tggctctgat tagtattgaa acagggattg aaaaccaaca agacctagaa gacttatata
      421 cttactacat gagaatggat gccgtttctc ttttatctag tgatttagaa accagtcctc
      481 aagaggttga aatggagata gagttgtagg ggaatcatgt gattctccta ttttcgtata
      541 gatgaaagga gcgagtatgg aagtaataca attattggct atgtttcgtg gaacaattcc
      601 aaaagatagg gataaaatgg acctatttct tcgctatcag gcgcaacatt ttgatgagaa
      661 atggcaggac ttggtagaga gttttttgac tgaagaggag aagatagaag agatacctca
      721 tgtctattcg tttcatcaag acattgtttc tttcctagag gccagttctg aaaataatga
      781 ccaagatcta gaaagttaca caagaaattt tggacaagca ggactagata aattatctca
      841 attaagtaat tttgagaaaa atttggtgct aaaagtcgca agctataacc tttccactcg
      901 attttacatc caatctgaaa aagagaaact aacaccatta agtgagcttg tatttcatca
      961 gaatcaggat gttaatttag tcaatgtcta tcgggttgcg aataatctat ctgaccgtat
     1021 tagtagagat atagaggaat ttcttctaat ggttgactca aaagaggtta aaaaagaagt
     1081 tcctgagatt cattttgaag aaaaagaggg agatgttcta gcctatttgg gttcagaatt
     1141 gatggctact ttagatatcg ttacggatct tgtccatcat gaagaaaact acatacaact
     1201 cccactgaca caaaagctga agattattac tcattttgat gaagtaaaag ctaaaagcga
     1261 aaagtctaat caagtagaga aaattttatc tccttcaagt gatattgaac aggaaacgga
     1321 agaaactaac tccttttcta atgtcgataa aattgtagaa gaagctttga gggaatatcc
     1381 aatcggttca caagtaagtt ataaaggaca agtatttcag ttggtttcga ttgaaaatgc
     1441 gcagttaaat gacttagttc gcctagagct attcaatgat tccaaccagt tatttgaaga
     1501 gaatcctatc ttatacttga acagtttgga agagattgaa caagtattgt ctcatttaga
     1561 acttgaaaaa gaagattcag agattgagat tgattcatca agtgaaagtc aggaaataga
     1621 tttgttttca tacctggaag aagaaaatgt aaatgaaaag gataaggata aggaaacaaa
     1681 atctttaatt tcaggtatag aagagacgga tgtccctgtt ctagattttg tttttccaga
     1741 tgatttagag gacttttatc ctaagacaaa tcgagaaaag attgaaacga atatcgccgc
     1801 aattgaactt gttaaaagat tagaaaaaga gagacgacaa gcgaatccag aagaacaaga
     1861 gctactcgcc aagtatgtcg gctggggcgg tcttgccaat gaatttttcg atgaactcaa
     1921 tccaaagtat gaaacagaac gtttaactct taagagctta gtaagtaaat cagaatactc
     1981 gaccataaaa caaagttctc tcacagccta ttatacagac ccaatgatta ttcgccagat
     2041 ttggcaaaaa ttactggatg atggttttga gggaggaagg atattagatc cttctatggg
     2101 gactgggaac ttctttgcgg cgatgcctag aagtatacga gagaaatcag aactctatgg
     2161 ggttgaatta gacagtgtga ctggtgcaat cgcaaaacaa ctccacccca atacccatat
     2221 tgaagtgcga ggatttgaag aagttcccta tcaaaataat agttttgatt tagtcttaac
     2281 gaatgttcct tttggaaatt ttcgcattgc cgataaaaac tatgataaac cttatatgat
     2341 tcatgattac tttgtcaaac actcacttga tttagtaaga gacggaggac aagtgtcgat
     2401 tatctcatct attgggacaa tggataagcg gacagataat gtcttacaag agattaaatc
     2461 caatactcat tttttagggg gagttcggtt gccggatacg gcttttaaaa agattgcagg
     2521 tacccgagtg accacagatc tcctattctt tcaaaaggat caagcaaaga atcttaatga
     2581 ggaggaactt gtctttagtg gctctgttcc ctttgaggag gataagcgtg tctggatcaa
     2641 tccttatttt gatgggaaat acaatacaca agttttgggt gaatatgagg tacgtaattt
     2701 taatggagga accctcaatg ttaaggggga atcagaaaca ttagctactg acataatgaa
     2761 agcattagag aatgtagaag cacctaaaca aattgacaat tctttgaaag cacctgtttt
     2821 tatccaagaa gaagtggata attctatccc aagtcatata cgtgagaact tagcgctcta
     2881 ttcttttgga tatgagggaa atcaaattta ttatcgagat acgcatggca ttcggagaag
     2941 ttcaaaagta gacgaaatta gttattatgt agatgagaag ggagatttta aagcttggga
     3001 cagttccttg tctgaacata aaatagatcg attcgtgcaa cttcatttga cagatgagga
     3061 agcactagat gtatacaagt cagaagaagc gagtaaaaga gggaaatata agggcttgtt
     3121 caaaaaaacg gtcttttatg aaagcccctt atcggataag gatattagtc gcattaaggg
     3181 catggttgat ttgagagaga cctatcaagc cttaattgaa attcaacgcc atccagatta
     3241 tagtcggaca gattttcagg tattacttag taaactcaat cgtgactatg atcggtttgt
     3301 aagccaattt ggatacttga atgtctcagt caatcgaaac ttatttgata gtgacgataa
     3361 gtattcttta ctagcaagtt tagaagatga atacattgat tctaaagatc agaaagtaaa
     3421 atataaaaaa tctttagcct ttgagaaagc attggttagg ccggagagag tgattacaag
     3481 agtttcaacg gctttagatg ccttaaactc cagtttatca gatggtagag gggttgatat
     3541 agactttatg gtatcaattt accctgaaca tagccaagca gctattttag atgagttggg
     3601 tgaccagatc ttaatggatc cagaaagcta tttaagaggg gaaagaaatt atctttctaa
     3661 gaaccagttt ttgtcaggag atattctcaa caagatagaa gtagttcaac tattagtaga
     3721 ggaaaacaac caagaatgtg attggactca tgcgttagat ttgttagaat ctgttcgccc
     3781 tccaaggatt catctggcag atattgagtt taaaataggg tcacgttgga ttcctcaatc
     3841 cgtttatggt aaatttgcct ttgaatgttt taccaaccgt gaatttgaat tgtcttcgcc
     3901 tgatgttgaa caagtcattg aagtgaatcc tgtcgatggg caggttcatt taaggacacc
     3961 atttgcttat cgctatccaa gtgccaaaga tagtagtctt ggagtcagtg gctcacgtta
     4021 tgatacagga agaaagattt ttgagaattt acttaattcg aaccaaccga ctattactat
     4081 gactgttacg gaaggggaaa agaaaaagac catcacagat ttggaaaaaa cctctgttct
     4141 aagagcaaaa gagcagcatt tacaagaact ctttcaagac tttgtctcac ggtatccaga
     4201 agttcaacaa gtcattgaag aaagttataa tcgtctttat aatcgaacgg ttagtcgaga
     4261 gtatgacggt agccatttag tcattgatgg cttggcacaa aacatcagtc ttcgtcctca
     4321 tcaagagaat gccattcaaa gaattgtaga agaaaaaaga gccttgttag ctcatgaggt
     4381 aggttcagga aagaccttga ccatgcttgg tgctggcttt aaattaaagg agttggggat
     4441 ggttcataag cccttgtatg tggtgccctc tagtttgtct gctcagtttg gccaagaaat
     4501 catgaaattt ttccctacta aaaaagtctt tgtgaccact aagaaagatt ttgtgaaggc
     4561 aagaagaaaa cagtttgtgt cacgtattat tacaggagat tacgatgcca ttgtcattgg
     4621 ggattctcaa tttgaaaaaa tccctgtcag taaggaaaga cagatgaatt atatcgagga
     4681 taaactcaat gaactacgag agattaaaac acattctgaa aataagtaca ccgttaaaga
     4741 agcagagcaa tcaataagtg gtcttgagaa acaattggaa gaactccaac gctttaatcg
     4801 tgatagtttt attgattttg agaacttagg aattgatttt ctctttgtgg atgaagcaca
     4861 tcactttaaa aatatacgtc caattactgg acttggaaat gtagcaggga ttaccaatac
     4921 aacgtctaag aagaatgtgg atatggaaat gaaggttcga cagattcagg aagaacatga
     4981 ttttaaaaat attgtctttg cgacaggaac acctgtttca aattcaatta gtgagctgta
     5041 tactatgatg aactacattc aaccggatat cttaaaacgc tatcaagttg attattttga
     5101 ctcttgggta ggtgcttttg gagaaattca aaactctatg gaattagctc ctacagggga
     5161 taagtaccag cctaagaaac gatttaaaaa gtttgtcaat ctacctgagt tgatgaaaat
     5221 ctataaagaa acagccgaca ttcaaacaca agatatgttg gatttacctg taccagaagc
     5281 ccatattatc cctattgaga gtgagttaac tgaaaaccag aaactctatc tagaagaatt
     5341 ggttatgaga tcagatgcgg tcaaatgtgg aacagttgat ccaagtcagg ataacatgtt
     5401 aaaaattacg ggtgaggcac gaaaattagc tattgatatg cgtttattgg actctagtta
     5461 tagtctagca gacaatcata aactgcttca ggtagtggat aatgttgaaa gaatttatcg
     5521 tgagggaatg gaaaataagg ctactcagat gattttttca gatattggca cacctaagaa
     5581 aaaagataat ggctttgatg tttattctga gattaaggct ttattagttg atagaggaat
     5641 ccctagtatg gaaattgcct ttgtacatga tgccaatagt gatgaaaaga agaatagctt
     5701 gtctcgaaag gttaatgcag gagaggttcg ggttcttctt gcctcaactg aaaaaggagg
     5761 aacaggttta aatgttcaga gcaagatgaa agcagttcac catctggatg taccgtggag
     5821 accaagtgac attcagcaac gtaatggacg tattatccga cagggaaatg aaaacaagga
     5881 agtggatatt taccactata ttaccaaagg ttcgtttgat aattatctat gggcaactca
     5941 ggagaacaaa ctccgttata ttaagcagat tatgacttct aaggagccga ttcgtgctgc
     6001 ggaagatatt gatgaacaga ctatgacagc ttctgatttt aaggcactag caacaggtaa
     6061 tccttatctc aaatataaga tggaactaga gaatgatcta actctattag aaaatcaaag
     6121 acgcgccttt caacgcagca aggatcacta tcgtcataca atctcttact gtgaagaaaa
     6181 tatgcccatt cttgagaaac gattaagcaa gtatgaaggc gacattcaac agtctgaaat
     6241 gtcgaaagac caatcatttt ctatgacgat aggtaaacaa gtttttgagc aacgagctga
     6301 agcaggtgaa tccctacacc gtcttatccg tcataatcaa tctgacagca aagaatttcg
     6361 aaccctagca agttatcgag gatttgacat taaaatgctt agtcttgcaa caaatcaacc
     6421 tcttcctgaa accttctctg ttaagattgt aggagaaaac caatattctg tcagtttaga
     6481 tttgtattct cctttgggga caattcaaag gcttcagcat acgatagacc acattaaaga
     6541 tgaccaagtg aaaactcaga acttattgga tgaattaaag gataaatgga ctactgctaa
     6601 ggtagaaatt gagaaaaatt ttccaaagga agaggattat caaactaaaa aggccgaata
     6661 cgatgtactc gcgccattga ttgaaacaga aacggattta gatattattg atcaggcctt
     6721 acgacaattc cacgaaaaag gaaaagaaaa gcaagaacaa ctttcttttg aattagatta
     6781 aaaaatagat ataaatagcg gacaagaaaa gaaaagcgcg gtagaataaa gatagaaaga
     6841 aacgaggtaa cgattatgat ggaagatacc tattatcaat tagaagaggc tttggtacag
     6901 ggatttcaaa cacctgaaga ataccaagcc tacaaggagt taaaggaaca ttatgaggaa
     6961 gtgacagggg attacagttt ttctaaacga gagctcacta gtcaattgga aatcgctctt
     7021 cagaatcatc aaggtgtgga ctttgaagaa catgaaaaag aagagtattt ggacttagtt
     7081 caaaaattag aagagtttga ttcctctctt gccacccatt atcgtcagtt gattgattag
     7141 aaaggagaaa gtcatgaatg atttactcct tatcccagta atttttttag cagtaggagg
     7201 aattatcatt cttttatgga gactctttct tattgccagt ggacttttcc ttattggttt
     7261 tgtcagtttt cttattttcg tagaggtcta tggaatttat ctgttcttta ctgaacctag
     7321 tttatatttt gatgatatca gacaatatgg tttaactagt tttacggctg tgtacctttt
     7381 catcaatctg atgttggttc taggatttag ttggcgcttc attaactcca taaataagca
     7441 taaaatgtga actttttaga tagttaaaag actttattca aaggtaagtt tgaataaagt
     7501 ctttttgttg atgtgcttat aatcccgtct tagtacttag tgtgagaaac aaaaagagat
     7561 gaattaaaat taaagtgtac agaatgctta agagtttcta tttttcttat aatatatagt
     7621 aatttcggta ca
//
LOCUS       DAVKWS010000073         7490 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKWS010000073 DAVKWS010000000
VERSION     DAVKWS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.074"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            1..530
                     /locus_tag="VW852_001875"
                     /pseudo
     CDS             1..530
                     /locus_tag="VW852_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            616..1566
                     /locus_tag="VW852_001876"
     CDS             616..1566
                     /locus_tag="VW852_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0272960.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVFSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1743..2750
                     /gene="gap"
                     /locus_tag="VW852_001877"
     CDS             1743..2750
                     /gene="gap"
                     /locus_tag="VW852_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV0272961.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2817..3653)
                     /locus_tag="VW852_001878"
     CDS             complement(2817..3653)
                     /locus_tag="VW852_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0272962.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3779..5974
                     /locus_tag="VW852_001879"
     CDS             3779..5974
                     /locus_tag="VW852_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0272963.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQVVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6027..6179
                     /gene="rpmG"
                     /locus_tag="VW852_001880"
     CDS             6027..6179
                     /gene="rpmG"
                     /locus_tag="VW852_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0272964.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6189..6365
                     /gene="secE"
                     /locus_tag="VW852_001881"
     CDS             6189..6365
                     /gene="secE"
                     /locus_tag="VW852_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV0272965.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6420..6956
                     /gene="nusG"
                     /locus_tag="VW852_001882"
     CDS             6420..6956
                     /gene="nusG"
                     /locus_tag="VW852_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV0272966.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            7078..>7490
                     /locus_tag="VW852_001883"
     CDS             7078..>7490
                     /locus_tag="VW852_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV0272967.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFR"
BASE COUNT         2364 a         1290 c         1628 g         2208 t
ORIGIN      
        1 acacctacta tgaacaagac ccagaaaaag tagccttatt tcttaaaaat tttaatagtt
       61 taaagcacct agcacctgtt tagattgatg aaacaggatt cgatacttat ttttatcgag
      121 aatatggtcg ctcattaaaa ggtcagttaa gaagaggtaa agtatctgga agaagatatc
      181 agaggatttc tttggttgca ggtctaacaa atggtgagtt aatcgctcca atgacttacg
      241 aagagatgat gacgagcgac ttttttgaag cttggtttca gaagtttctc ttaccaacat
      301 taaccacacc atcggttatt attatggata atgcaagatt ccatagaatg ggtaagttag
      361 aacttttatg cgaggagttt gggcataaac ttttacctct tcctccctac tcacctgagt
      421 acaatcctat tgagaaaaca tgggctcata tcaaaaagca cctcaaaaag gtattaccaa
      481 gttgcaatac cttttatgag gctcttttgt cctgctcttg tttcaattga ctatagttca
      541 cggatacagt tgggaaagaa gctattaaat atttggagag tccttttggg atgaacatgt
      601 caaatattac tatagatggg caagaaaaga tgggcaagaa atggatctga gtcaaacgat
      661 gcttcttatg ctcaagtagt gagtctttat gatgatacaa gtatctctgt tagtaataat
      721 gagacggaca aagtgttgac tggatccctc tatactgaaa caaatgaaca gggattaacg
      781 attccaagca gtttactaaa aaattggaat gaacagacag gaaaaaattt gacaactaat
      841 gatcttattg gcaaatcagt ctcagccagc attgtagaaa gtgctgccga aactagtaag
      901 attgctcaat ttcaaacgaa gattgtacgt gtaatcaatg atgaagatga catggaggac
      961 agcaacagtt tcatgctgtc tcatcaaatg gaaacgattt tgaaagaggc tggatttacg
     1021 aaagctgtat cttattttat cttggaactc aaagatccat cacagacaaa agtagtaaca
     1081 gaagaattac agaaaaataa gaagtatact gtgttttctc aacagagagt tcttgatatt
     1141 gtgattacct ttattcgtgt tattcaggga ttattgattg tgctttcatc acaagctatt
     1201 gtggtagcag cggttatgat tggtatcatt atttacatca atatcatgca acgttccaag
     1261 gaaataggtg tcatgaaagc agttggttat cagaatcgtg gtgtcaaagg aatttttatt
     1321 tacgaggcta tctggattgt aggcatcgcc ttgctgctgg catttttggt tgcacaaggg
     1381 gtgggaagtt tggcgaatgc gattgtaagt cacttttacc catccatcac taaggttttt
     1441 gaattaaatc ttttatctgt tttaggaact ctagttttcg ctctattact tggttatgtc
     1501 tcagcctact tcccggcgcg taagattagt aaaatggatc ctgtagaatc gttacgctat
     1561 gaataataag ggattccttg atttacagtc ttaaaaagaa actcacatag aaaaacaatg
     1621 gccatacttg caattcaact caaatagttg tataataggt ttgttgaaag gtgatttgta
     1681 gtgattcaag ttactctttt cacaataaaa atttcatgat tttcataagg aggaaatcac
     1741 taatggtagt taaagttggt attaacggtt tcggacgtat cggtcgtctt gctttccgtc
     1801 gtatccaaaa cgtagaaggt gttgaagtta cacgcatcaa cgaccttaca gatccagtta
     1861 tgcttgcaca cttgttgaaa tacgacacaa ctcaaggtcg tttcgacggt actgttgaag
     1921 ttaaagaagg tggattcgaa gttaacggta aattcgttaa agtttctgct gaacgtgatc
     1981 cagagcaaat cgactgggct actgacggtg tagaaatcgt tcttgaagct actggtttct
     2041 ttgctaagaa agaagcagct gaaaaacacc ttaaaggtgg agctaaaaaa gttgttatca
     2101 ctgctcctgg tggaaacgac gttaaaacag ttgtattcaa cactaaccac gacgttcttg
     2161 acggtactga aacagttatc tcaggtgctt catgtactac aaactgcttg gctccaatgg
     2221 ctaaagctct tcaagataac tttggtgttg ttgaaggatt gatgactact atccacgctt
     2281 acactggtga ccaaatgatc cttgacggac cacaccgtgg tggtgacctt cgccgtgctc
     2341 gcgctggtgc tgcaaacatc gttcctaact caactggtgc tgctaaagct atcggtcttg
     2401 taatcccaga attgaacggt aaacttgacg gatctgcaca acgcgttcca actccaactg
     2461 gatcagttac tgaattggta gcagttcttg aaaagaacgt tactgttgat gaagtgaacg
     2521 cagctatgaa agcagcttca aacgaatcat acggttacac agaagatcca atcgtatctt
     2581 cagatatcgt aggtatgtct tacggttcat tgtttgacgc aactcaaact aaagttcttg
     2641 acgttgacgg taaacaattg gttaaagttg tatcatggta cgacaacgaa atgtcataca
     2701 ctgcacaact tgttcgtact cttgaatact tcgcaaaaat tgctaaataa ttcttgagtt
     2761 gatagaaagc aaggctttgt ggtcttgctt tttatatgga aaaatggatg acacgatcat
     2821 ccattctttt ttaattcttt ttcaaatgta tttgaaaggg tagtgaaagt tagcttctct
     2881 aaagtaagtg ggtgggtaaa ggaaagtcgg aaggcatgaa gcataagccg acttgtcttt
     2941 gatttactat tatagagagg gtcgcccagg ataggaagat tatgatgcga aaggtgcaca
     3001 cgaatctgat gggttcgccc tgtctttagc ttgcaacgag ccaaggaagt cttgtttgag
     3061 aattgcttta acctgcttac atgcgtttca gcatattgcc cattttttgc atcaactatt
     3121 ctttttctac gatcatggcg atcacgtcca attttgtctc tgaaaacaag ttcttttctg
     3181 ttgatatttc catcaactag agcccaatat tctctagaaa tctctttttt ctccaataag
     3241 cgattgagaa tgggcaggat aaaaggattt ttggcaaaga gaactaagcc actggtttcc
     3301 atgtccagac gatgaacgac atagcaggtt tggccaacat aggtactgac atggttaaga
     3361 agggcaattt cgtttggttg attaccatgc gttttcatcc cctctggttt gtttacaata
     3421 atcaagtgtt gatcttgata aacttcctgc actaagtctg ggttgcccca agggatcgtc
     3481 ttttgggaat aatcttcctc gtcaaaagtc aactggcaaa catctccagg atttacgatt
     3541 tcgttccagt ggacttcttc ttgatttatc aaaatatgtt tcttgattct caaaaaatga
     3601 cggatttttc tagggatgag gagttgttcc tcaagtaatt gctttaccgt catttgaggt
     3661 agagaggcgg gtaatgtaaa tgtgaattgc atacagatat tgtaacaaaa aaagccctat
     3721 ttggatagga aatagctaaa ttcttgtctt cctatgatga agatgataaa ataaacgcat
     3781 gaaattagat aaattatttg agaaatttct ttctcttttt aaaaaagaaa caagtgaact
     3841 agaggactct gattctacta tcttacgtcg ctctcgtagt gatcgaaaaa aattagccca
     3901 agtaggtccg attcgaaaat tctggcgtcg ttatcatcta acaaagatta tccttatact
     3961 aggtttgagt gcaggcttgc tagttggaat ctatttgttt gctgtagcca agtcaaccaa
     4021 tgtcaatgat ttgcaaaatg ccttgaaaac tcgaactctt atttttgacc gtgaagaaaa
     4081 agaggctggt gccttgtctg gtcaaaaggg aacctatgtt gagctgactg acatcagtaa
     4141 aaacttgcag aatgctgtta ttgcgacaga agaccgttct ttctataaaa atgacgggat
     4201 taactatggc cgtttcttct tggctattgt cactgctggc cgttcaggtg gtggctctac
     4261 cattacccaa cagctggcta aaaacgccta tttatcgcag gatcaaactg ttgagagaaa
     4321 agcgaaagaa tttttccttg ccttagaatt aagcaaaaaa tatagtaagg agcaaattct
     4381 aaccatgtac cttaacaacg cttattttgg aaatggtgtg tggggtgtag aagatgcgag
     4441 taagaaatac tttggagttt ctgcatcaga agtgagtctg gatcaagctg cgactctggc
     4501 agggatgctc aaggggccgg aactgtataa tcccttgaat tccgtagaag attctactaa
     4561 tcggcgcgat actgtcttgc agaatatggt tgcagcagga tatattgata aaaaccaaga
     4621 aaccgaagct gctgaagttg atatggcttc gcaattgcac gataagtatg aaggaaaaat
     4681 ctcagattac cgttacccct cttattttga tgcggtggtt aatgaagctg tttccaagta
     4741 taatctaaca gaggaagaga ttgtcaataa tggctaccgc atttacacag agctggacca
     4801 aaactaccaa gcaaatatgc aggttgttta tgaaaacaca tcgctatttc cgagggcaga
     4861 ggatggaacg tttgctcaat caggaagtgt agctctcgaa ccgaaaacag ggggagttcg
     4921 tggagttgtc ggtcaagttg ctgacaatga taaaactgga ttccggaatt tcaactatgc
     4981 aacccaatca aagcgtagtc ctggttctac aattaagcct ttagttgttt atacgccagc
     5041 agttgaagca ggctgggctt tgaataagca gttggataac cataccatgc agtatgacag
     5101 ctataaggtt gataactatg cagggatcaa aacgagtcga gaagttccta tgtatcaagc
     5161 cttggcagaa tcgcttaatc tacctgctgt tgccactgtt aatgatttgg gtgtcgacaa
     5221 ggcttttgag gcaggcgaaa aattcggact caacatggaa aaggtcgacc gtgttcttgg
     5281 tgtcgccttg ggaagcggtg ttgaaaccaa ccctcttcaa atggctcaag catacgccgc
     5341 ctttgcaaat gaaggtttaa tgcctgaagc tcattttatt agtagaattg aaaatgctag
     5401 tggacaagtt attgcgagtc ataaaaattc acaaaaacgg gtgattgata agtctgtagc
     5461 tgacaagatg accagtatga tgttggggac tttcaccaac ggtaccggta ttagttcatc
     5521 gcctgcagac tatgtcatgg cagggaaaac tggaacaact gaagcagttt tcaatccgga
     5581 gtacacaagt gaccagtggg taattggtta tactccggat gtagtgatta gccactggct
     5641 tggctttccg accactgatg aaaaccatta tctagctggc tctacttcaa acggtgcagc
     5701 tcatgtcttt agaaacattg ccaataccat tttaccttat acgccaggaa gtacctttac
     5761 ggttgaaaat gcttataagc aaaatggaat tgcaccagcc aatacaaaaa gacaagtaca
     5821 aaccaatgat aatagccaga cagatgataa tttgtctgat attcgagggc gtgcgcaaag
     5881 tctagtagat gaggctagcc gggctatctc agatgcgaag attaaggaaa aggctcaaac
     5941 aatatgggat tcgatagtca atctatttcg ctaagatgct tgtcaaagcc tagctttctt
     6001 gttataatgg ataagatgga ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg
     6061 tttgtggttc gagaaactat tcaatcaaga tcagcggaaa ccccaagcct acacgactag
     6121 aagtaaataa attttgtaag cattgtggca agtacactac acacagagaa acgagatagg
     6181 agagagcgat gcgttttatt ggagatattt ttagacttct taaagacaca acatggccaa
     6241 ctcgcaagga aagctggaga gattttcgtt ctatcatgga atacacagct ttctttgtag
     6301 taattattta catttttgac cagttgattg tttcaggttt gattcgattt attaacattt
     6361 tttagaagat tagtggagtt aattacacta gaaatcttct atttatgaaa ggaaatatca
     6421 tggatagttt tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg
     6481 tgaaagaaaa tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg
     6541 ttgaaattcc aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag
     6601 aaaatcgctt tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg
     6661 ttgttcgaaa cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac
     6721 caactccatt attggaacaa gaaattcgcg acattttggt atctatggga caaactgttc
     6781 aagaatttga tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg
     6841 cagactacac tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta
     6901 tgtttggtaa tgacacagtt gcagaagtaa atctaaacca aatcgcagaa ttataaccct
     6961 gagagaggct ggaccctctc ttttttgtgc agtttaggag ctaaagggaa cagaatggag
     7021 aaaatggaac aaatgtgttt tctaatctgt tagactgtat ctagaaaggg gaaaattatg
     7081 attaaagaat tgtatgaaga agtccaaggg actgtttata agtgtagaaa tgaatattac
     7141 cttcatttat gggaattgtc ggattgggac caagaaggca tgctctgctt acatgaattg
     7201 attagtagag aagaaggact ggtagacgat attccacgtt taaggaaata tttcaaaacc
     7261 aagtttcgaa atcgaatttt agactatatc cgtaagcagg aaagtcagaa gcgtagatac
     7321 gataaagaac cctatgaaga agtgggtgag atcagtcatc gtataagtga ggggggtctc
     7381 tggctagatg attattatct ctttcatgaa acactaagag attatagaaa caaacaaagt
     7441 aaagagaaac aagaagaact agaacgcgtc ttaagcaatg aacgatttcg
//
LOCUS       DAVKWS010000074         7455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKWS010000074 DAVKWS010000000
VERSION     DAVKWS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.075"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            126..281
                     /locus_tag="VW852_001884"
                     /pseudo
     CDS             126..281
                     /locus_tag="VW852_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            481..3756
                     /gene="rexB"
                     /locus_tag="VW852_001885"
     CDS             481..3756
                     /gene="rexB"
                     /locus_tag="VW852_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV0272968.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3753..7403
                     /gene="addA"
                     /locus_tag="VW852_001886"
     CDS             3753..7403
                     /gene="addA"
                     /locus_tag="VW852_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV0272969.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDINHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEIFANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2274 a         1567 c         1583 g         2031 t
ORIGIN      
        1 atattagatt gaaataagat acgaacagct tgattaggga aatcaaatta atttctagaa
       61 atattttagc atccacgatg tactattata gtttcaattt actatagttc aatcattcta
      121 tcaagaatat tgaatatata aaggcacgta atattaaaac ttcttctgat atcgaattag
      181 agtccgatgt tgatggcgat aaatcagaca atctacccgt cgacattaag atactgggac
      241 aacacattga agtgtattcc gtacctaaga aacaatcata aaactcaaag tcttgttacc
      301 gaggcttttt tattatgtga ttttcagtag ccagacttat tctatctaat acaagtaaag
      361 ctaagtattt aagatttagc agatgatgaa gaaaagaaag gatggcctaa atattttaac
      421 agatttacga ggctaatcat ggtataatat agggtgatga aaaattccaa cgaggctgag
      481 atgaaattac tttatactga tattcgaact tctttgacag aaattctaac aagagaggca
      541 gaagagctag ttgcagctgg caagcgggtc ttctacattg cccccaactc tctttctttt
      601 gaaaaggaac gcgccgtgct ggaatgcttg tcccagcagg cttctttttc tattaccgtc
      661 acgcgctttg ctcaaatggc tcgttatctg gtcctgaatg atttaccagt taaaactact
      721 cttgatgata tcggtcttgg gttggccttt tacaaatgcc ttgccgaact cgatcccaag
      781 gacttgcgtg tttatggcgc tattaagcag gatcctcaat tgatccagca gttaattgag
      841 ctttaccatg agatgaccaa atctcagatg agttttttgg acttggagaa tttaacagat
      901 gaggataaga ggacggattt actcttgatt tttgagaaag taacagccta tcttaatcaa
      961 ggtcagttag cccagggaag tcagttgtcc catttgattg aggctattga gaatgacaag
     1021 gtaagtagtg attttaatca aatcaccttg gtcattgacg gctttactcg tttttctgct
     1081 gaggaagagc gggttgtgga cttacttcac ggcaaaggtg ttgagattgt tatcggggct
     1141 tatgctagta agaaagccta taccagtcct tttagcgagg gcaatctcta ccaagccagc
     1201 gtaaaatttc tccatcatct ggcttctaaa taccaaacgc ctgctcagga ctgttctcaa
     1261 actcatgaga agatggatag ttttgacaag gcttctcgtt tgttggagtc ttcttatgac
     1321 ttttcagaac tcgctttgga tgtcgatgag aaagaccgtg aaaatttaca aatctggtct
     1381 tgtttgacgc aaaaggagga gttggagcta gtagcccgta gcattcgtca gaaattacat
     1441 gagaactcag acctgagcta caagcatttt cgtattctct tgggggatgt agcttcttac
     1501 cagttatctc tcaaaaccat ttttgaccag tatcagattc ctttttatct tggtagaagc
     1561 gaagccatgg ctcatcatcc cttgactcag tttgtcgagt ctattttagc tttaaaacgt
     1621 taccgttttc gtcaggagga tttgattaat cttcttagaa ctgatttgta tactgacctc
     1681 agtcagtctg atattgatgc ttttgagcaa tatatccgct atcttggtat caatggcttg
     1741 ccagcctttc agcaaatctt caccaaatcc caccatggaa aatttaatct tgagcgtttg
     1801 aatgtcctcc gcctgagaat tttagcacct cttgaaaccc tctttgccag ccgaaaacaa
     1861 aaggctgaaa acctcctaca aaaatggagt gtctttctaa aagaaggagc tgtgaccaag
     1921 cagttacaag atttgacaac cactttggaa gctgtagaac aggaaagaca agccgaagtt
     1981 tggaaggctt tctgccatgt tttagaacaa tttgcgactg tttttgctgg ttcacaggtt
     2041 agtctggaag acttcctagc cttgctccat tctggaatga gtttgtccca ataccgtacc
     2101 attccagcaa cagtggacac tgttctggtg cagagttacg atttgattgc accattgact
     2161 gctgactttg tctatgctat tggactaact caggacaatt taccaaaaat ttctcaaaac
     2221 accagtcttc tgacagatga agaaaggcaa aatctaaacc aagcgaccga agaaggcgtt
     2281 caattactga ttgccagcag tgaaaatctc aagaaaaatc gctacactat gctttccttg
     2341 gtcaattctg ctcgtaagca gttgttcttg tcggctccaa gcctttttaa cgaaagtgaa
     2401 agtaaggaat ctgcctatct tcaagagttg atccattttg gatttaggcg gagagagaag
     2461 aggatgaatc acaaaggact gtctaaggag gatatggggt cctatcacag tcttttgtct
     2521 agtctggttg cctatcacca gcagggtgag atgagcgata ctgagcaaga tttgactttt
     2581 gtcaaggttc tgtcgcgtgt cataggtaaa aaactagatc tgcaaggtct ggaaaatcca
     2641 gctatcccaa ccagtccaag cagcaagacc ttagccaagg acaccttgca agctctctat
     2701 cctgccaaac aggagtttta cctgtctacg tcgggtttga cagagtttta tcgcaatgaa
     2761 tacagttatt tcctacgcta cgttttaggc ttgcaggagg aattacgttt gcgtcctgat
     2821 gcccgtagtc acgggaattt cttgcatcgt atctttgaac gcgccttaca gttgcctaat
     2881 gaagattcct ttgaccaacg tctagaacaa gctattcaag aaaccagtca agaacgcgaa
     2941 tttgaagcta tttatcaaga aagtttggaa gcccagttta ccaaggaagt tttgcttgat
     3001 gttgcacgga cgactggaca tattctccga cacaatccag ccatcgaaac catcaaagaa
     3061 gaagcaaatt ttggtggaaa agaccaagcc tttattcaat tagacaatgg acgcagtgtc
     3121 tttgtacgag gcaaggtgga ccggattgac cgtttgaaag ctaatggagc gataggagta
     3181 gtagactaca aatccagtct gactcagttc cagtttcctc atttctttaa tgggctcaat
     3241 tctcagttac caacctatct tgctgcccta aaaagagaag gggagcagaa ctttttcggc
     3301 gccatgtact tggaaatggc tgaacctgtc caatctctga tggcggtaaa aagtctggca
     3361 ggagcagtgg tagaagccag caaatctatg aaataccaag ggctcttctt ggaaaaagaa
     3421 agcagttatt taggcgaatt ttataacaaa aacaaggcta atcaactgac agatgaggaa
     3481 tttcagctcc tactggacta caatgcctat ctttacaaga aagctgctga gaagatttta
     3541 gcaggccggt tcgccatcaa tccttatact gaaaatggca gaagcattgc cccatacgtc
     3601 cagcaacatc aggctattac aggctttgaa gccaattacc atctgggcca agcccgtttc
     3661 ctagaaaagt tggacctagc tgatggcaag cgtctggtcg gagaaaaact caagcaagct
     3721 tggtttgaaa aaataagaga ggagttaaat cgatgaagct tattcccttt ttaagtgagg
     3781 aggagattca aaaactgcaa gaagcagaag caaattcgag caaggaacag aagaaaactg
     3841 ccgaacaaat cgaagctatc tacacttctg gtcagaatat cctggtctca gcatcggctg
     3901 gttctggaaa gacctttgtc atggtagagc gcattctgga ccaattggcg cgtggtgtcg
     3961 aaatttctca actctttatc tcaaccttta ccgtcaaggc tgcaactgaa cttaaagaac
     4021 gtttagagaa aaaaatcagc aagaaaatcc aagaaacaga tgatgtcgat ctcaaacaac
     4081 acttgggtcg ccagttggca gacctaccca acgctgccat cggaaccatg gactctttca
     4141 cacaaaaatt ccttggcaaa catggttatc tgcttgatat tgcacctaat ttccgtattt
     4201 tacaaaacca aagcgagcaa cttcttctcg aaaacgaagt ctttcatgag gtctttgaag
     4261 cgcattacca aggtaaacag aaagagagct ttagtcattt gctgaaaaac tttgctgggc
     4321 gtggcaagga cgaacggggt ctgcggcagc aagtctataa aatctatgac ttcctccaat
     4381 ccaccagtaa tcctcaaaag tggctgagtg aatctttcct caaaggcttt gagaaagctg
     4441 attttaccag tgaaaaagaa aaactgactg agcaaatcaa acaagccctc tgggatttgg
     4501 aaagcttttt ccgttaccat ctggataacg atgccaagga gtttgcaaag gctgcctatt
     4561 tagaaaatgt tcagttaatt ctggatgaaa ttggctccct aaatcaggag ttcgatagtc
     4621 aggcttatca ggcagtactt gcgcgtgttg tcgccatctc taaggagaaa aacggtcgag
     4681 ctctgactaa tgccagccgt aaggctgatt tgaagcccct ggctgatgcc tacaacgaag
     4741 agagaaagac ccagtttact aaactaggac aattatcaga ccagatagcg attctcgact
     4801 atcaagaacg ttatcatgga gacacttgga aactagctaa aaccttccaa tctttcatga
     4861 gcgattttgt agaggcttat cgtcagagaa aacgacagga aaatgccttt gaattcgctg
     4921 atatcagcca ttacaccatt gagattttag aaaatttccc acaagttcgt gagtcttatc
     4981 aggagcgctt ccatgaagtc atggtcgatg agtatcagga tatcaaccat attcaagaac
     5041 ggatgctgga attgttgtct aatggccaca atcgctttat ggtgggagat atcaagcaat
     5101 ccatctatcg tttccgtcag gcagacccgc agattttcaa tgagaaattc caacgctatg
     5161 cgcaaaatcc tcaagaaggc aagctcattc tcctcaagga aaatttccgt agtagttcag
     5221 aagtgctgtc agcaaccaat gatgtctttg aacgtctcat ggaccaagag gtcggcgaaa
     5281 tcaactatga taacatgcac cagcttgttt ttgccaatac caaactgact cccaatccag
     5341 acaacaaggc cgaatttctc ctctacgaca aggacgatac aggtgaggaa gaagagagtc
     5401 aaacagaaac gaaactaaca ggcgaaatgc gcttagttat caaggagatc cttaaacttc
     5461 atcaggaaaa aggtgttgcc tttaaggaaa ttgcccttct gacctctagt cgtaatcgta
     5521 atgaccaaat tctacttgcc ctgtctgagt acgggattcc tgtcaaaact gacggagagc
     5581 aaaacaatta tctccaatcc ctagaagtgc aagtcatgct agacaccctt cgtgtcattc
     5641 acaatcccct gcaagactac gccttggttg cccttatgaa gtctccaatg tttggttttg
     5701 atgaggatga gctagcacgt ttgtcccttc agaaagcaga ggataaagcc cacgaaaatc
     5761 tctatgagaa actagtcaat gcacaaaaaa aggcaagtag tcaaaaaggc ttgattcaca
     5821 cagctctagc tgaaaaacta aagcaattca tggatatcct agcttcttgg cgcttgtatg
     5881 ccaaacccca ctctctctat gacttgattt ggaagattta caacgaccgt ttttattatg
     5941 actatgttgg ggctttgccg aatggtcctg ctaggcaggc caatctctat gccctagcac
     6001 tgcgtgctga tcaatttgaa aagagcaatt tcaaaggttt gtcgcgtttt attcgtatga
     6061 ttgaccaagt cttagaagcc cagcacgatt tggcaagcgt ggccgtcgca ccgccaaaag
     6121 atgcagtaga gctcatgacc atccacaaga gtaaagggct ggagtttcct tacgtcttta
     6181 tcctcaatat ggatcaagat ttcaacaaac aagactctat gtcagaagtc attctcagtc
     6241 gtcagaatgg tcttggtgtc aaatatattg ccaagatgga gacaggggca gtagaagacc
     6301 actatcctaa aaccatcaaa ctctccattc ctagtctgac ctataggcag aacgaagagg
     6361 aattacagct agcaagctat tctgagcaga tgcgtttgct gtatgttgct atgacgcggg
     6421 ctgagaaaaa gctctatctt gtcggcaagg gttctcgtga aaagctggaa tccaaggaat
     6481 acccagcagc caaaaatggg aaactaaata gcaatactag actgcaagca cggaatttcc
     6541 aagattggct ttgggctatc agtaaagtgt ttactaagga caagctcaac tttagttatc
     6601 gttttattgg cgaagatcag ttgaccagag aagctatcgg agagttggaa accaagagtc
     6661 ctctccaaga tagctcccaa gcagacaatc gtcagtcaga taccatcaaa gaagctctgg
     6721 aaatgctgaa ggaggtggaa gtttataata ctcttcaccg cgcagctatt gaacttccta
     6781 gtgttcaaac cccaagtcaa atcaagaaat tctacgaacc agttatggat atggaaggtg
     6841 tcgagattgc tggtcaaggt cagtcagtag gcaagaaaat cagcttcgat ttgccagatt
     6901 tttcaaccaa agaaaaggta actggagctg agattggtag tgctactcac gaactcatgc
     6961 agagaattga cctcagccag caactaaccc ttgctagcct aacagaaaca ctcaaacaag
     7021 ttcaaactag ccaagctgtc agagacaaga tcaatcttga taaaattctt gctttctttg
     7081 acacagcact cggtcaggaa atttttgcta ataccgacca tctctatcgc gagcaacctt
     7141 tctccatgct caaacgagac caaaagagtc aggaagactt tgttgtccgt ggtatccttg
     7201 atggctatct gctttacgaa aacaaaattg ttttgttcga ctacaagaca gaccgctatg
     7261 atgaaccaag tcaactcgta gaccgctatc gtggtcagtt agctctatac gaagaggctt
     7321 tatcacgagc ctattcgatt gaaaatattg aaaaatactt gattttactc ggtaaagacg
     7381 aggttcaagt tgtaaaagta taacttatac tcttcgaaaa tctcttcaaa ccacgtcagc
     7441 ttcgccttgc tgtat
//
LOCUS       DAVKWS010000075         7448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKWS010000075 DAVKWS010000000
VERSION     DAVKWS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.076"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..1438)
                     /gene="pfbA"
                     /locus_tag="VW852_001887"
     CDS             complement(<1..1438)
                     /gene="pfbA"
                     /locus_tag="VW852_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV0272970.1"
                     /translation="MKYFVPNKVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDS"
     gene            complement(2601..3827)
                     /locus_tag="VW852_001888"
     CDS             complement(2601..3827)
                     /locus_tag="VW852_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV0272971.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGANLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3870..4562)
                     /locus_tag="VW852_001889"
     CDS             complement(3870..4562)
                     /locus_tag="VW852_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0272972.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALIL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4735..6486)
                     /locus_tag="VW852_001890"
     CDS             complement(4735..6486)
                     /locus_tag="VW852_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272973.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLVAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(6476..>7448)
                     /locus_tag="VW852_001891"
     CDS             complement(6476..>7448)
                     /locus_tag="VW852_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0272974.1"
                     /translation="TLLVGSLMVQEGQITVGNLVTFISYLDMLVWPLMAIGFLFNTTQ
                     RGKVSYQRIENLLSQESPVQDPEFPLDGIENGRLEYAIDSFAFENEETLTDIHFSLAK
                     GQTLGLVGQTGSGKTSLIKLLLREYDVDKGTIYLNGHDIRDYRLTDLRSLMGYVPQDQ
                     FLFATSILDNIRFGNPNLPLSAVVEATKLARVYQDIVDMPQGFDTLIGEKGVSLSGGQ
                     KQRLAMSRAMILEPDILILDDSLSAVDAKTEYAIIDNLKETRKDKTTIITAHRLSAVV
                     HADLILVLQNGQIIERGRHEDLLALDGWYAQTYQSQQLEMKGEEDAE"
BASE COUNT         2238 a         1578 c         1427 g         2205 t
ORIGIN      
        1 cactatcttt tgcaactcgt atcgcttttg ttttaggatc ggcaatatta aatatatttt
       61 cggcgataac cacctgttta tttaaatcta ataggtcttt agtgttttta tagctataag
      121 catttactaa agctgctccg ttttctcgat aatgtacact ctctccttta actttcttat
      181 caaagcgatt tcctttgatt aatacatcag tgaatcctgt aaaacgtaca cctgcataca
      241 tcatgttatc aaaatgatta tttaaaattt taatattaga ggggttctgt gtcgacaatg
      301 tttgatagtg tgtgccaatt gctgttacta attccccaga tttatcactt ttgccaaaat
      361 aggaattttg aatagtcaca ttttcagatt ttttcccatc atcattcaag gcataaggaa
      421 aaccttttct agttaatggt tcaatctgaa tgctctcctt acttatgatt tgcccatcct
      481 tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt atcaactaat acatttttcg
      541 aacctgcaat ttgaatagca tgcccttgat aactatcctt gaatgttaca ttttttatag
      601 ttacgttatt tgaattccca atagcaaatg cacctgaaga atttataagt ggtagatttt
      661 ttgctttagt tccttcttca ttcaaagcac cgttcatatc aatcgtacca ccagaataac
      721 taatatcttc tgttgggccc cattctactt gcgcaccatc atccgtaaat aaacctgtca
      781 taaaaacaat ggaagggtga ttcttaatat ttataccatt tagaattgta gctttctcat
      841 tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc ttttactaaa taagttcctt
      901 caggaaaata tacatttcct ccacctagcc cttgagctgc agcatctatt gcatcttgaa
      961 ttgcttgtcg atcatcatga atcccatcac ctaccgcacc ataatctttt acacttaaag
     1021 cagtatcttc aaattctttt ttgataattc tgtcacttat aacatcttcc ttataactct
     1081 tattttcatt agtagatgtt actgtagatt caactgttct gttgtctaat tgtgaattgg
     1141 aacgaatagt accattagag ctgatactat taacacttaa cgaattatct aaaccattag
     1201 tggttgaatt agaggcaatc atttcagcag aacgattttg attaacagtt ggtgaattat
     1261 ctaaatcatt agtaattgca ttagaagact cttttgtagc catcggtgaa gaactagtaa
     1321 caacttcatc cgataaaata ccttggcttc ccaaaattga aattgccaat agtaccgagc
     1381 aagtccccac ctttaattta cgaatactga ataccttatt aggaacaaaa tatttcatca
     1441 gaacctccaa tttttttact atttttaaca attgattatt acttttctta aaatagatag
     1501 aatgacaccg ctgtcattat ctttatcata tattttccaa attgaattgt caaggaataa
     1561 cacaagaaaa tattaaaatt tatactcttc ggaaatctct tcaaactagg tcagtgccgc
     1621 cttgccctaa gtctaggtta ctgacttggt cagttctata gtagactgaa actataatag
     1681 tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcccgat cgatttgtcc
     1741 tattcttatt tcattttact atatctacaa cctcaaagca gggttttgag ctatctatgg
     1801 ccaacttcct agtttgctct ttagccttcc attcaaaagt gatgctatct ttcttagctt
     1861 gaatataaaa tatcccattt actatattta aatttgttga tatgttgtaa attataaatg
     1921 aaagaacaaa tatcttgtca tacaagcatt cgccagggat atttacctga atatcgctca
     1981 tatggcggat tgaaactaaa acactacaat atatttctta aatagacctt ctataaaagc
     2041 tagatttaac ctcgataaaa atagatgcag tgcttctaaa atactggaat agggcatata
     2101 ggtaatcgta gacaagtgtt ttatagattg tctcgcggga gatctaatga tgtatagcaa
     2161 gcatcactaa acatatagaa tatttgtatt attataatat caatatgcta taaattctat
     2221 catgcaaaag aatgactaga acatgaaaga caggcacaaa actttgaaca atattctgaa
     2281 aacaaaaaaa gacgcgagtt tccacaatct cactcatgga aactcacgtc tttcaaaaaa
     2341 tagggtgaat tgaaaactag aacaagacac tagattttct aaatgatgtc tagtaaggat
     2401 tctaaatgct acaccaaatt gctcacatct tatttcaaac cacgggactc ccacaagaaa
     2461 ggaaaatcct ataaaaattc tctctctgag gagaaaggag cctcaactac aggtgtaaac
     2521 tattatcaat tgaaaacaac agtttttaca ctcaattaga ctttttaggg acaggatcac
     2581 tttcgcttga ctgtagtttg tcattttttt gataaagtta gcactttttc agaaactgtt
     2641 ttgaaagtct caatgatata gtctacttct tcatcgctta atttagtatg aagagggagg
     2701 gtaatttcat tctcaaagaa ggcataggcc ttaggatagt tcgtcatatc aaatccaaga
     2761 ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa cattacttgc aattcctgct
     2821 ttagccaatt cttggatgat gaggttgcgt tcttctaggt ttgctccttc tacacgggtg
     2881 atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt gtgccaaagg atggatgcga
     2941 gaacctgcaa aaccactatc atagcggtcc acaatgtcct tacggcgttg caacaaactt
     3001 ggatagcggt ccaattgtac caaaccaagt gaagccatga tatcggtcat gttgcactta
     3061 taggctggtg taacgatatc gtattcccat gaccccaatt gcatcttggc aagagcatcc
     3121 ttagtttgcc cgtgaaggga aaggatttgg aattccttgt acatctcttc gtcatcaatc
     3181 actggattgg ctttccaagt cgcacttcca ccttctgccg ttgtaaagtt cttaacggca
     3241 tggaatgaga aggaagtaaa gtcagcgata gaaccagaag gttgtccttt ataagtagat
     3301 cccaaagcgt gggcactatc agagacaatg acaatacggt taaaggcctt ttgccacttg
     3361 cttgaagcgg taaagaagtc acgttttttc tccacgactt ggaacaaacg gtcataatcg
     3421 caaacaatcc ctgcgagctc tactgggata atcaccttag ttttctcagt gatagcttgc
     3481 tcaagcaggt catagtccat ctcaaacgta tctgcttgga tatccaccat gacaggggtt
     3541 gctcccacgt gcgtaatgac actacatgaa gccgtatagg tcatggctgg aacgatgact
     3601 tcatcaccag gtcccacttc caaaacgcgt aaaatcaact ccagagcagc tgtcgcagag
     3661 ttgagacaaa cagtcttagg tgtctgtgtg taaagagaca agcggcgctc cagttctttt
     3721 gttttaggac ctgttgtgat ccaaccagaa cgcagggtat ccgctacttc agcaatttct
     3781 gcttctgtga tatcaggcgg tgaaaatgga atattgtaat ttggcattga tttgctcctt
     3841 ttatctgtac tcattttctt atgactactt tattttagta cctcaaacac ggtttgaaac
     3901 atgattttga tgtccccaaa gaaactaaac tctcggagat aggcgaggtt atagcgcatc
     3961 ttttcaggaa gaacatgctc cacataggcc tgatcaactg acagaccttt ctccgtcatt
     4021 tgactgatga tggtgtcctc atccttgtag ttgatgctgg ctggagaggt aattcctgct
     4081 tgcaagagca gggttgccat catttcaggg ctatactgct ctgtataacg tggcacttca
     4141 ggtcgtgtac cgacaaagga catctcacct ttaaggacat tgaccaactg aggcagttca
     4201 tccaaacgga cacgtcggat gaaatttcca accttggtaa tacggctatc gttagcagaa
     4261 gtcaccagac ttcctttttt atccgcatcc gtcaccatgg tacgaaactt ccaaatcttg
     4321 aaccgacggt tgtactgggt cacacgctct tgcttgtaaa tcactggccc cttgctatcc
     4381 aacttgatcc aaatgctcaa gatgagaaag atgggagagg tcagaaccag taagatcaag
     4441 gccaaaaccc agtccaagca acgcttgaaa atcagcgaac ccttcctttt agagacaagc
     4501 tggtagtaag actcaacctc gcttgatttc atttccacag gcaagtcttc ccatttcagc
     4561 atgctgttct cctttgtttt tattatacta tttgtcaaca tttttcatta taccacaaaa
     4621 tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt ccttctagta ctcaatgaaa
     4681 atcaaagtac agctttgaga ttgcagataa agctgacatg gtttaaagag attttcaaag
     4741 agtatcggcc atggcccctg cctgcaaact atacatcttg tgataggttc ctcccagagt
     4801 caagagttcc tcatgggttc cactctcgat aatgcgtccc ttatccaaga catagatgca
     4861 gttggcgtct tgaatagtag aaaggcggtg agcgatagca atagttgttc ggccctgtct
     4921 catcttcgcc agagaagctt gaaccaagct ttctgtttca gagtcaatat tggctgtcgc
     4981 ttcatccaaa atcaggattt taggctggct ggcgactgtt ctagcaaagg caagaagctg
     5041 gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca ggggagtcgt acccctgagg
     5101 aagttcttga ataaaggaat ctgcatccac aaaggctgcc gcagcctgaa cctgctcatc
     5161 actgatttct tggtacatgg cgatattgga cttaatagtt ccatgataga ggaagggttc
     5221 ctgtaagacc aaaccgatgt tttttctcag ctcttcttga ctgaaatccc tgatatccac
     5281 atcatccaag agaactctcc ctgactggaa ttcataaaag cgcatgagga cattgataat
     5341 agacgatttc cctgaacctg tatgacctac aaaggcaatg gtttcaccct tattaacaga
     5401 gaaagaaatg tcatccagaa tcggatgttt accgtcatac gagaaacaca catgttcaaa
     5461 acggatattg ccttctttga ctttagcctg cccattttct tgaaggggct cataggtcct
     5521 ctcatcgatt aaggcaaaga cacggcctgc agaaaccata gacgtttgca gtgttgaaaa
     5581 gttttgcgtc acctcaatca aggggtcaaa gaggcggttg atgtactgga taaaagcata
     5641 catggtcccg gccgttatcc cgatagaaag accacggtag ccaaaatagg ccaccaagac
     5701 agcataacct agaagtttca acaaactcat ggcaggtctc aaaaagaggg catccaaggc
     5761 tacagaacgg ttggcataga ccaagtgctc ttggttgatt tcatcaaatt ctgcctgcag
     5821 gcgcttctct tgattaaagg cctggattat tctgattccc tcgatgtttt cagccagctt
     5881 gctattgata tctgacaaga gacttctggt tttctcaatg attttcactg attttttccg
     5941 atagagattg accaaaagga aaatcaaggg gagaaagagc aagactaatg ctgtcaaacg
     6001 aaaatccaat accaacatgg tataaagggt tgtcagaaag atgaaaactg ctgagataaa
     6061 gctagataaa atccccgaaa acatatcact aatcgtctcg gtatcgttgg tcaaacgaga
     6121 aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg cccagtttct ccatattggc
     6181 aaaggcatcc cgacgaatat ccctaacaat actgtaagac acgcgcgcaa agagaagatt
     6241 gccgacatac tgaactacag tttgtaggat gtagagacca tagtagacca gcaaaacggt
     6301 aacggctagt tggttgagat tgctgagata ctggtcgata aagtgggaag ccacgagggg
     6361 tatgacactt ttaatgaccg tcgtcgctag gagaaaactg agtgccaaaa aggtcaggag
     6421 tccataaggc ttgagataag acatcaagcg cttcaataca gcccattgtt cttgtttatt
     6481 ctgcatcttc ttctcctttc atttccaact gctgagactg gtaggtttgg gcataccagc
     6541 catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat aatttgacca ttttgcagga
     6601 ctaaaatcaa atctgcatgg acaacagcac tcaggcgatg ggcagtgata atggttgttt
     6661 tgtcctttcg cgtctccttg aggttgtcga taatcgcata ctctgtcttg gcatctacgg
     6721 cggataagga atcatccaaa atcaagatat caggttctaa aatcatagcc cgactcatag
     6781 ccaaccgttg cttttgacca ccagaaagac tgactccttt ttcaccaatc agcgtatcaa
     6841 atccttgagg catgtctaca atatcttggt aaactcgggc tagcttagta gcttccacga
     6901 ccgctgaaag gggcaagtta ggattgccaa agcggatatt gtctaggatt gaagtcgcaa
     6961 agagaaactg gtcctgagga acatagccca tgagactgcg aaggtctgtc agacgatagt
     7021 cccgaatatc gtgaccgttt agataaatag tacccttatc cacatcgtat tcacgcaaga
     7081 ggagcttgat taaggacgtt ttcccagagc ctgtctgccc aaccaagccc agtgtttgcc
     7141 cttttgccaa actaaagtga atatccgtca gtgtttcctc attttcaaaa gcaaagctgt
     7201 caatggcata ctccaaacgc ccattttcaa taccatccag aggaaactca gggtcttgta
     7261 caggagattc ctgagacaaa agattttcaa tccgctggta agaaaccttc cctcgctgag
     7321 tagtattaaa gaggaagccg atggccataa gaggccagac cagcatatcc aaatagctga
     7381 taaaggtgac tagattccca actgtaatct gcccttcctg aaccatcaag gagccaacca
     7441 aaagcgtt
//
LOCUS       DAVKWS010000076         7069 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKWS010000076 DAVKWS010000000
VERSION     DAVKWS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.077"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..239
                     /locus_tag="VW852_001892"
     CDS             <1..239
                     /locus_tag="VW852_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0272975.1"
                     /translation="LNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLAL
                     QNEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            269..3619
                     /locus_tag="VW852_001893"
     CDS             269..3619
                     /locus_tag="VW852_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0272976.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYCATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEEQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3863..3964
                     /locus_tag="VW852_001894"
                     /pseudo
     CDS             3863..3964
                     /locus_tag="VW852_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4206..4676)
                     /locus_tag="VW852_001895"
     CDS             complement(4206..4676)
                     /locus_tag="VW852_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0272977.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4693..6966)
                     /locus_tag="VW852_001896"
     CDS             complement(4693..6966)
                     /locus_tag="VW852_001896"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV0272978.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2173 a         1198 c         1520 g         2178 t
ORIGIN      
        1 atctaaatca gaaatttatt atccatgttt taaggaataa taattatagt cgagtgatat
       61 caggaagtgc tcagcctcag ttaccaatta caaaattaaa aaaaatactt ctccccctcc
      121 ccccactagc cctccaaaat gagttcgcag actttgtagt ccaggtcgac aaatcacaat
      181 ttgcttgtga gatagctata aaagtgtgga gaaatagctt gaaatttagt ataatatagc
      241 taaactattt gtttaaagtg agaaaaaaat gggaaatttt agctttcttt taaaaaatga
      301 cgaatatgaa tctttttcaa aaccttgcat tgaagctgaa aatatgattg ctacatcaac
      361 tgtggctact gcctttatgg cgcgtcgtgc tttggagcag gctgtccatt ggatatatag
      421 tcacgattca tatttagaag ctccctattg tgctactcta tcttctttag tatgggatga
      481 tgattttagg gatatcgtag attctgaact ccacaagcag atagttctgt tgattcggtg
      541 gggaaaccat gctgctcatg gtggtgaaat taaggaacga gaagcgattt tagctttaca
      601 tcatttgtat cagtttgtta attttatcga ttattgttac agcaatgaat ttgtggagcg
      661 ttattttgat gagaagtgtt taccactttc agcaaacatc aaataccgag aaactccaca
      721 atctatgata aagttacaag acagtttacc agaactgcct gattttcatg aacagatggc
      781 tgctcagtcc gtagaagttc aagagactta tactgaaaaa cgtgagactg cagcgcaacg
      841 gcaagatgtg cctttccata ttgaacaatt atctgaggca gagacaagaa agctctttat
      901 tgatatcgat ctccgtttag caggatggat atttgaagaa aactgtcgtg ttgagatagc
      961 cgttgatggt ctcaagcacg gttcaggaat tggttactgt gactatgtac tttatggtaa
     1021 aaatgggaaa attttagcga ttgtggaggc taaaaaagcc tctgtcaatc cagaagtagg
     1081 ggaagtacag gtcaaagaat atgctgaagc tttggagaaa catatcggct atcagccaat
     1141 ttgctttatt acaaatgggt tgaagcacta tatacttgat ggtccgaacc gccgccagat
     1201 tgcaggcttt tactctcaag aagaattgca attagtgatg gatagacgtc atcttcaaaa
     1261 accgcttgag gatatttcta gtaaaattag ggacgatatt tccgggcgtc actaccaaaa
     1321 acatgccatt gcaagcgttt gtgaagcttt ctctgatcat cgtagacagg cacttttggt
     1381 tatggcaact ggggctggga aaactcgtac agcagtttct ctagttgata tcttatcacg
     1441 tcataactgg gtaaaaaacg ttctcttctt agccgataga acttccttgg ttaagcaagc
     1501 atatgattcg tttagaaaat tactcccaga tctttccgtt tgtaacttct tagaagataa
     1561 agaaggagct caatcaagtc gcatggtctt ttcaacttat ccgaccatga ttggagcgat
     1621 tagtggtcaa gaagaagtaa atcaacgccc tttcactgtt gggcattttg accttatcat
     1681 aattgacgaa tctcaccgtt ctatttatca gaaatacaag tccatttttg attattttga
     1741 tgcaagaatt gtaggcttaa cagctactcc gcgtcaagat ttagataaaa acacctatgg
     1801 attctttaat ttggagaatg gggttccaac atatgcatat gatttggaag aggctgttaa
     1861 agacggatat ttagtagcct atcattctat cgaaaccaaa ctgaaactac ctacggatgg
     1921 tctacattat gatgatttgt ccgaagaaga aaaggaacat tttgatagca aatttgaaga
     1981 cgatagctgt gaaaaagata ttgatgggag tgtatttaat tcctttgttt tcaataaaag
     2041 tacagtagaa attgttttaa atgaactcat gacaagagga attcagacag cctcgggtga
     2101 tgaaattggt aaaactatta tttttgctaa aaatcatgat catgcggaat atatcagagg
     2161 tatttttaac aaccgctatc ctgaaaaagg gagcgactat gctcaggtga ttgattatag
     2221 tattaagcat tatcagacct tgattgatga ttttaaaatt aaggagaagt atcctcaaat
     2281 tgcgatttct gtcgatatgt tagatacagg tattgatgta ccagaggttg ttaatttagt
     2341 cttcttcaag aaagtacgct ctaaaactaa gttttggcag atgattggtc gaggaacccg
     2401 tctatgtaaa gatttatttg gacctgagca ggataaggaa aacttcttgg tatttgatta
     2461 tggggacaat tttgattatt ttcgtgcaga tccaagagat ggagagggtc gtcacattgt
     2521 ttcgttgact cagcgtttat ttaatatcaa agtggacttg attcgagaac ttcagggact
     2581 ccaataccaa gaagaacagt ttgcgagagc ataccgtcag cagcttgtct cggaacttca
     2641 aggtcgtata gagagcttaa atgagttgga cttcagggtt cgtatggttt tagatacagt
     2701 ttatagctat aggaaattgg aaagttggca gaatctaact gctgttacaa gtgaaaccat
     2761 tcaaaaaaat ctctctccgc ttttatttga tgaagataaa gaagatgaga tggcgaggag
     2821 atttgatttg tggttgcttc atattcagtt ggggcaactg acagctaaat cttccactgt
     2881 tcatatttcc caagtgatga agacggctag agctctttct gctattggca atatcccgca
     2941 ggtttttgag caggctgaaa ttatcaggaa agtacaggag cctgaatttt ggaaagaagt
     3001 taacttgtct gatttggaaa aaattcgtct tgctattcga gatttattac agtttttgga
     3061 taaaacagac cgtaaaccct actatgttaa ctttgaagat cgtatactct ccactgttca
     3121 cgagaccaca gcatttttgc aggtcaacga tcttcggtct tacaatgaaa aagttgagca
     3181 ttatttgaaa actcatctgg atgaggagtc catttctaag ctataccata ataaaaagtt
     3241 gacatctgat gatatgcttg cacttgaaaa attgctttgg gaaaaattag gtagtaaagc
     3301 agactaccaa agtcattatg aaaataaggc aattccgaga ttggttcgtg agattattgg
     3361 cttagataga gagtctgcca atcgtatttt ttctaaattt ttgtcggatg agaatcttaa
     3421 tgccaggcag atttcatttg taaaattgat tgtagactac attgtagaaa atggtttttt
     3481 agagacgaaa gtgttaacgc aagagccgtt taaatcttat ggttctgttc aactactctt
     3541 ccaacaccaa ctaccagtac ttcgtaatat tgttcaaatc attgaactta tcaataatcg
     3601 agctggagaa gcggcttaaa ttctaaagtg attgccatgc tgagactcat ttaaaattaa
     3661 aaagagtaga aatttatgct atatatgaga agttttatta ggaagaatgt catcgttttc
     3721 ctagaataca gtatcagttg ttaagtggct gataaatttc aaagtagata cttgtaccac
     3781 gatgtttgtt gatagagtta ttaacaaaag agctactttg attttaaaga aatagaaaac
     3841 aaaaagccga gcaagaattc aattgcagga gaaaatgaaa taatactcaa tgaaaatcaa
     3901 agagcaaact aggaaactag ctgcaggctg ctcaaaacac tgttttgagg ttgtagataa
     3961 gactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg acgaagtcag
     4021 taaccatacc tacggcaagg cgacgttgac gcggattgaa gagattttcg aagagtataa
     4081 atcttcctag gataaagcaa aacgcatagt atcaagggtt ttcaacactt gatactatgc
     4141 gttttctgat gttaaagact ttctaccagt tttttttaag cataattgtt agttgtagtc
     4201 atttattatt cttcaaagaa aaatggtggg gcgaattttt tcagttcttc aaagcacttt
     4261 tgagcagtat ctgcatcttc acagatgata agacagacat cattaccaca aagggtagcg
     4321 atagcgtcag ggaagctcaa agtatcaatg atagaaccaa aggattgagc cagtccagga
     4381 agggttttta gtaggacttg gtgttgaact gggcgcatcc agacaagggc gtcttccatg
     4441 tagagttcga gacgtttttc ccattttgag atggaaccat tgttaagaac ataataagcg
     4501 ctatcttctt cgcggacttt tgataggttc atatttttga tgtcgcgtga gagggttgcc
     4561 tgggttactt gaatgtcgtt cttagcaaga agggcttgca actcagcctg tgtatgaatc
     4621 ttgttttttg tgataagagc gcgtataagt tggtggcggt gttctgattt attcataata
     4681 atgtaactcc ttttagcaag gtaaggtaag catggactga gcgaggtcga cagtcaagtg
     4741 gtagtctgta ttgtcacgga tggtgatttc aaagtcagta gtatagagga ctaaacggag
     4801 agtgtctcct tcttttagct tgtaaatagt tggctgcagt tcaaattgaa cgtccatcca
     4861 ttcatctgca gtaatatcct ctactaacag taaatcattt ctattttgta aattaaggta
     4921 accttttgtc acgactcgtt gtgcctctgg tctaaatggc aattcacaga gattttccaa
     4981 catgtgatag cgaccgttgt caatggttct agcacttaaa atagctggat aaggttgtag
     5041 gtatttcttt tgcccaaatt ctagcagttg ggcagataag agccccttgt ttgtactgga
     5101 tttgatacga agattgagct gagcgcgacc gtttaggtgg agatctttag tcacaggaag
     5161 gttaatagta atctgattgg ctttcccttg atagagctct gtattgaagg tttggtatgt
     5221 cttaccatag cgctcaaaat ccttatctgg gtactggttt tgaatagctt gctcttcttg
     5281 accaagtgag aaggtttcac agttttcttg cccaccgaag ttatcaagtg ataaccaagt
     5341 ctgtggagct gtattgtcct gccagataac agtaggaagt tgaaagtctg tttcctgtcc
     5401 tagtaatttc ttggttaata aggcatttat ggactcacgg aagtcaattg attgccaatt
     5461 gttcatgtaa acatgggcac cattatggaa aaagagatgc ttgtgtatat gagtaggaag
     5521 agcatggaac atctggtaaa catgaagtgg tttgacattc caatcctgag aaccatgagt
     5581 aaagacaacc tctgccttta ctttatgggc attgagcaga taattgcggt catgccaaaa
     5641 ctgattgtag tccccagttt ttcggtctag ctgagctttc actttttcta agtcagcttg
     5701 gtgagcttca ttgccacgga tatagtcgcc agctaagaga ttacgagaat aggttaactc
     5761 agcaagggag tcaaagtcct cacctggata accacctggg ctagtcacca gaccgttttc
     5821 acggtagtag ttgtaccatg atgaaattcc tgcctcggca atgataactt ctaaaccatc
     5881 gactcctgta gtcgcaagac cattggacat ggtacctaga taggaaagtc ctgttgtagc
     5941 aacttttccg tttgaccaat cagccttgac ttgacgctgg cgcgtgtgat cagtaaaggc
     6001 acggcaacga ccgttaagcc aatcgatgac atttttataa gcctcgattt gctggtagtc
     6061 tccattagtc atgaaacctg tcgagtcttt ggtaccaaca cctgagacat agagattggc
     6121 aaagcctctc ggaaggaagt agtcgtttag tgtatagcta gagttgatgt gagttagctt
     6181 ttcctcagcc tctgctataa gctcagcttt accttggggt tggacgagat ttagttgagg
     6241 tttctctagc tcaatcttgt gaggaagctt aacctcaagc tcgccctcca tcttgtagag
     6301 agccttgtca ctagccttgt cattggttcc ctgatgataa gggctggctg tcatgatggc
     6361 agggattttt ccatcaaaac gagggcgaat aatgctaacc tttactaggt ctgatagccc
     6421 tttttggtca gtatcgacac gagactcaac gtaaacgact tcacgaatga catcctggtt
     6481 agaaaaagta gccaaactct tgccgttaaa gtagtggtag tcattatcct ccggaataag
     6541 accatcacta acaagttggt cgataagagt atttcctttt ttggtgcgag tattgagtaa
     6601 ctgatagaga ttttcaatca agtcaccata tataatggga aatccagttt ctttacgaaa
     6661 aacgtcacta tcttcgaagt caaccaaata agaaaagcct aaaagttgaa aagcaacagt
     6721 ataaaaaata tctgctgtca gttcatcttc tgattgaaaa aatgtcagca ggtctgtttt
     6781 tttatcagct gctaggatag aaagtgggta gttggtgtct tgataagtga aaaagaaacg
     6841 acgtaaaaag gtttcaagtg agtctttgtg attggctgta ttttgtaaat caaagccaca
     6901 tttttttagt tcagataaga cattttcttt tggaaaattg atataactat attgattaaa
     6961 acgcatagaa cctccatata gaatgacagt taaggttatt atatcaaaaa aaaagcagaa
     7021 agggaattgt taacttcaaa aggaaataat ccaataaaaa tgaataaag
//
LOCUS       DAVKWS010000077         6969 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKWS010000077 DAVKWS010000000
VERSION     DAVKWS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.078"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            87..6518
                     /locus_tag="VW852_001897"
     CDS             87..6518
                     /locus_tag="VW852_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV0272979.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDNNTSNEEARIKE
                     DSNQSQGDNAHSSANKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSSLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVS
                     REHFIRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEG
                     KSLFNIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAY
                     DDGTDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEI
                     KATKYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKET
                     KSEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQ
                     SVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYINGKEYTSFNDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2739 a          975 c         1343 g         1912 t
ORIGIN      
        1 tacttagtaa aactttttat aacaaaggct agcaaagtta aagtttatta tctattggaa
       61 gttatataaa tatgtaagga attaaaatga aaaaaagtac agtattgtca ttaaccacag
      121 ctgcagttat tttagcagcc tatgccccta atgaggtagt cttagcagac acatctaact
      181 ctgaagatgc tttaagcatc tctgataaag aaaaagtagt agtagataag gaaacagaaa
      241 ataaagagaa acataaagat attcataatg ctatagaaac ttcaaaggat actgaagaaa
      301 agaaaacaac aattattgag gaaaaagaag ttgttagtaa aaatcctgtg atagacaata
      361 acactagcaa tgaagaagca agaatcaaag aagattccaa tcaatctcaa ggagataatg
      421 cacactcgtc tgcaaataaa aacacagaaa atcccaaaaa agaagataaa gttgtctata
      481 ttgctgaatt taaagataaa gaatctggag aaaaagcaat caagcaacta tccagtctta
      541 agaatacaaa agttttatat acttatgata gaatttttaa cggtagtgcc atagaaacaa
      601 ctccagataa cttggacaaa attaaacaaa tagaaggtat ttcatcggtt gaaagggcac
      661 aaaaagtcca acccatgatg aatcatgcca gaaaggaaat tggagttgag gaagctattg
      721 attacctaaa gtctatcaat gctccgtttg ggaaaaattt tgatggtaga ggtatggtca
      781 tttcaaatat cgatactgga acagattata gacataaggc tatgagaatc gatgatgatg
      841 ccaaagcctc aatgagattt aaaaaagaag acttaaaagg cactgataaa aattattggt
      901 tgagtgataa aatccctcat gcgttcaatt attataatgg tggcaaaatc actgtagaaa
      961 aatatgatga tggaagggat tattttgacc cacatgggat gcatattgca gggattcttg
     1021 ctggaaatga tactgaacaa gacatcaaaa actttaacgg catagatgga attgcaccta
     1081 atgcacaaat tttctcttac aaaatgtatt ctgacgcagg atctgggttt gcgggtgatg
     1141 aaacaatgtt tcatgctatt gaagattcta tcaaacacaa cgttgatgtt gtttcggtat
     1201 catctggttt tacaggaaca ggtcttgtag gtgagaaata ttggcaagct attagggcat
     1261 taagaaaagc aggcattcca atggttgtcg ctacgggtaa ctatgcgact tctgcttcaa
     1321 gttcttcatg ggatttagta gcaaataatc atctgaaaat gaccgacact ggaaatgtaa
     1381 cacgaactgc agcacatgaa gatgctatag cggtcgcttc tgctaaaaat caaacagttg
     1441 agtttgataa agttaacata ggtggagaaa gttttaaata cagaaatata ggggcctttt
     1501 tcgataagaa taaaatcaca acaaatgaag atggaacaaa agctcctagt aaattaaaat
     1561 ttgtatatat aggcaagggg caagaccaag atttgatagg tttggatctt aggggcaaaa
     1621 ttgcagtaat ggatagaatt tatacaaagg atttaaaaaa tgcttttaaa aaagctatgg
     1681 ataagggtgc acgcgccatt atggttgtaa atactgtaaa ttactacaat agagataatt
     1741 ggacagagct tccagctatg ggatatgaag cggatgaagg tactaaaagt caagtgtttt
     1801 caatttcagg agatgatggt gtaaagctat ggaacatgat taatcctgat aaaaaaactg
     1861 aagtcaaaag aaataataaa gaagatttta aagataaatt ggagcaatac tatccaattg
     1921 atatggaaag ttttaattcc aacaaaccga atgtaggtga cgaaaaagag attgacttta
     1981 agtttgcacc tgacacagac aaagaactct ataaagaaga tatcatcgtt ccagcaggat
     2041 ctacatcttg ggggccaaga atagatttac ttttaaaacc cgatgtttca gcacctggta
     2101 aaaatattaa atccacgctt aatgttatta atggcaaatc aacttatggc tatatgtcag
     2161 gaactagtat ggcgactcca atcgtggcag cttctactgt tttgattaga ccgaaattaa
     2221 aggaaatgct tgaaagacct gtattgaaaa atcttaaggg agatgacaaa atagatctta
     2281 caagtcttac aaaaattgcc ctacaaaata ctgcacgacc tatgatggat gcaacttctt
     2341 ggaaagaaaa aagtcaatac tttgcatcac ctagacaaca gggagcaggc ctaattaatg
     2401 tggccaatgc tttgagaaat gaagttgtag caactttcaa aaacactgat tctaaaggtt
     2461 tggtaaactc atatggttcc atttctctta aagaaataaa aggtgataaa aaatacttta
     2521 caatcaagct tcacaataca tcaaacagac ctttgacttt taaagtttca gcatcagcga
     2581 taactacaga ttctttaact gacagattaa aacttgatga aacatataaa gatgaaaaat
     2641 ctccagatgg taagcaaatt gttccagaaa ttcacccaga aaaagtcaaa ggagcaaata
     2701 tcacatttga gcatgatact ttcactatag gcgcaaattc tagctttgat ttgaatgcgg
     2761 ttataaatgt tggagaggcc aaaaacaaaa ataaatttgt agaatcattt attcattttg
     2821 agtcagtgga agaaatggaa gctctaaact ccaacgggaa gaaaataaac ttccaacctt
     2881 ctttgtcgat gcctctaatg ggatttgctg ggaattggaa ccacgaacca atccttgata
     2941 aatgggcttg ggaagaaggg tcaagatcaa aaacactggg aggttatgat gatgatggta
     3001 aaccgaaaat tccaggaacc ttaaataagg gaattggtgg agaacatggt atagataaat
     3061 ttaatccagc aggagttata caaaatagaa aagataaaaa tagaacatct ctagatcaag
     3121 atccagactt atttgccttt aacaaccaag gtgttcatgc agaatccact agtggttcta
     3181 agattgctaa catttatcct ttagattcaa atggaaatcc tcaagatgct caactagaaa
     3241 gaggactaac tccatctcca cttgttctaa gaagtgcaga agaaggacta atttcaattg
     3301 taaacaccaa caaagaagga gaaaaccaaa aagaccttaa agttgtatca agagagcact
     3361 tcataagagg aattctaaat tctaagggaa atgacgctaa gggtatcaaa tcatccaaac
     3421 taaaagtttg gggtgacttg aaatgggatg gactcatcta taacccaaga ggtagagaag
     3481 aaaatgcccc agaaagtaag gataaccaag atccagctac taagatacga ggacaatttg
     3541 aaccgattgc ggaaggtcaa tatttctata aatttaaata tagattaact aaagattacc
     3601 catggcaggt ttcctatatt cctgtaaaaa ttgataacac agcccctaag attgtttcgg
     3661 ttgatttttc aaatcctgaa aaaattaagc taatcacaaa ggatacttat cacaaggtaa
     3721 aagatcaata caagaatgaa actttatttg ctagagatca aaaagaacat cctgaaaaat
     3781 ttgatgagat tgcaaatgag gtgtggtatg caggagctgc tcttgttaat gaagatggag
     3841 aggttgagaa aaatcttgaa gtaacttatg caggtgaggg tcaaggaaga aataggaagc
     3901 ttgataaaga tgggaatact atctatgaaa ttagcggagc aggagatcta agaggaaaaa
     3961 tcatcgaagt aatcgcccta gatggagcaa gcaactttac caaaatccat aggattaaat
     4021 ttgctaatca agctgatgaa aaagggatga tttcctatta tctagtagat cctgatcagg
     4081 attcatctaa ataccaaaag cttggcgaaa ttcctgaatc taaatttaag aatttaaaaa
     4141 atgttaaaga tgatagccta aacaaagaga cagctgaggt ggaaaataac cttttagtag
     4201 ataatcagtc tatcgaagga aaatctctct ttaatattca taaaactatt tcaacaatta
     4261 gagactttga aaataaagac ctaaagaaac tcattaaaaa gaaatataag caagaagacg
     4321 actttgtaaa tggtggaaca agaacagtag agcgtgatta taaatacgat gataaaggaa
     4381 atataatagc ctacgatgat gggactgatc tagaatatga aactgagaaa cttgacgaaa
     4441 tcaaatcaaa aatttatggt gttctaagtc cgtctaaaga tggacacttt gaaattcttg
     4501 gaaagataag taatgtttct aaaaatgcca aggtatatta tgggaataac tataaatcta
     4561 tagaaatcaa agcgaccaag tatgatttcc actcaaaaac gatgacattt gatctatacg
     4621 ctaatattaa tgatattgtg gatggattag cttttgcagg agatatgaga ttatttgtta
     4681 aagataatga tcagaaaaaa gctgaaatta aaattagaat gcctgaaaaa attaaggaaa
     4741 ctaaatcaga atatccctat gtatcaagtt atgggaatgt catagaatta ggggaaggag
     4801 atctttcaaa aaacaaacca gacaatttaa ctaaaatgga atctggtaaa atctattctg
     4861 attcagaaaa acaacaatat ctgttaaagg ataatatcat tctaagaaaa ggctatgcac
     4921 taaaagtgac tacctataat cctggaaaaa cggatatgtt agaaggaaat ggagtctata
     4981 gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagcc ctttcagaaa
     5041 caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa tctgtattaa
     5101 tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt aaaatgaacg
     5161 ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct aaacttgtat
     5221 tatttggtaa ggatgataaa gaatacactg gagaggataa gtccaatgta gaagctataa
     5281 aagaagatgg ctccatgtta tttattgata ccaaaccagt aaacctttca atggataaga
     5341 actactttaa tccatctaaa tctaataaaa tttatgtacg aaatccagaa ttttatttaa
     5401 gaggtaagat ttctgataag ggtggtttta actgggaatt gagagttaat gaatcggttg
     5461 tagataatta tttaatctac ggagatttac acattgataa cactagagat tttaatatta
     5521 agctgaatgt taaagacggt gacatcatgg actggggaat gaaagactat aaagcaaacg
     5581 gatttccaga taaggtaaca gatatggatg gaaatgttta tcttcaaact ggctatagcg
     5641 atttgaatgc taaagcagtt ggagtccact atcagttttt atatgataat gttaaacccg
     5701 aagtaaacat tgatcctaag ggaaatacta gtatcgaata tgctgatgga aaatctgtag
     5761 tctttaacat caatgataaa agaaataatg gattcgatgg tgagattcaa gaacaacata
     5821 tttatataaa tggaaaagaa tatacatcat ttaatgatat taaacaaata atagacaaga
     5881 cactaaacat taagattgtt gtaaaagatt ttgcaagaaa tacaaccgta aaagaattca
     5941 ttttaaataa agatacggga gaggtaagtg aattaaaacc tcatagggta actgtgacca
     6001 ttcaaaatgg aaaagaaatg agttcaacga tagtgtcgga agaagatttt attttacctg
     6061 tttataaggg tgaattagaa aaaggatacc aatttgatgg ttgggaaatt tctggtttcg
     6121 aaggtaaaaa agacgctggc tatgttatta atctatcaaa agataccttt ataaaacctg
     6181 tattcaagaa aatagaggag aaaaaggagg aagagaataa acctactttt gatgtatcga
     6241 aaaagaaaga taacccacaa gtaaaccata gtcaattaaa tgaaagtcac agaaaagagg
     6301 atttacaaag agaagatcat tcacaaaaat ctgattcaac taaggatgtt acagctacag
     6361 ttcttgataa aaacaatatc agtagtaaat caactactaa caatcctaat aagttgccaa
     6421 aaactggaac agcaagcgga gcccagacac tattagctgc cggaataatg tttatagtag
     6481 gaatttttct tggattgaag aaaaaaaatc aagattaaga taaaagctat agaaaaaaat
     6541 ggtttatgta ctgagattag atagtgaggt gatgacatag ttttgtgaaa acagccattt
     6601 ataactcaat tatttagttt acttcactat tgatactatt tggagttatt aatggactta
     6661 gtttagataa ctaatgaatt gattgaaagg gttagtattg acaatattgg tcatattgat
     6721 tagaaaatag agtctatcaa aatttaaagg ctaatagagg tgatgagaca atttcaagga
     6781 attaagtagc gtttggttgc ttaattcctt tttatttgga aggaagtttt tcgtaaccgc
     6841 ccaataacga agtctattgg acggttacga tatgatcact gacttcgtca gtcttatcta
     6901 caacctcaaa actgtgtttt gagcaacctg cggctagctt cctagtttac tctttgattt
     6961 tcattgagt
//
LOCUS       DAVKWS010000078         6754 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKWS010000078 DAVKWS010000000
VERSION     DAVKWS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.079"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..676)
                     /locus_tag="VW852_001898"
     CDS             complement(<1..676)
                     /locus_tag="VW852_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0272980.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFY"
     gene            complement(673..1998)
                     /locus_tag="VW852_001899"
     CDS             complement(673..1998)
                     /locus_tag="VW852_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0272981.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2019..2144)
                     /gene="comC"
                     /locus_tag="VW852_001900"
     CDS             complement(2019..2144)
                     /gene="comC"
                     /locus_tag="VW852_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV0272982.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(2309..2382)
                     /locus_tag="VW852_001901"
     tRNA            complement(2309..2382)
                     /locus_tag="VW852_001901"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2346..2348),aa:Arg,seq:tct)
     gene            complement(2426..2905)
                     /gene="rlmH"
                     /locus_tag="VW852_001902"
     CDS             complement(2426..2905)
                     /gene="rlmH"
                     /locus_tag="VW852_001902"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV0272983.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3088..4269
                     /locus_tag="VW852_001903"
     CDS             3088..4269
                     /locus_tag="VW852_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV0272984.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4327..5085
                     /locus_tag="VW852_001904"
     CDS             4327..5085
                     /locus_tag="VW852_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV0272985.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IKEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            5298..6659
                     /gene="dnaA"
                     /locus_tag="VW852_001905"
     CDS             5298..6659
                     /gene="dnaA"
                     /locus_tag="VW852_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV0272986.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2346 a         1212 c         1064 g         2132 t
ORIGIN      
        1 catagaaaac aatttctaag ttttttcgat caatttctct gatattgcct atatttacca
       61 aaaatgactt gtgaggagaa taaaatcgct gagtatgttt gtccttttcc tgaatatctg
      121 tcatggtacc ataaaactct tttgcaaaat tcttaccaat aatgcgcaat ttatgagata
      181 cccctgttgt ttcaatatac aaaatatcat ggtaaggaat ttttaaatca tttcccttgt
      241 aattgtagtc gaaataatct acaacatctt cattttcaag taacatactc ttcgtgtaga
      301 agatattttg ctcaattctc ttcttaaaca tctcatcatt gatatcctta tcaacaaaat
      361 ctagggctga tacctggtat ttataggtta gagtcgcaaa ctctgatcga ctagtgataa
      421 agacgataat agcgtaagga ttgtaatgac gaatgagctg agccacttca aatccctttt
      481 tctcaattcc atgaatatcg atatctagga aataaagctg atttacttca tcattttcaa
      541 tgtattcttc aaattcacgg acttttcccg ttgtcttgta tgatattgga atattcgatt
      601 ctttcgaaat ttcatccaat attctctcta gtctcacttg atgttcaata acatcttcta
      661 aaattaaaac tttcattcaa attccctctt aaatctaatg atttgtctaa atgtactgcc
      721 ttccatctct gtttctaaaa taatattgtt gtacttatct agtagttctt tcacattatt
      781 taatccgact ccgcgatttc ttcccttagt ggagaatcct aaggcaaata gatctcctga
      841 aggagtcatc gtcattttac atgaattctg aatcacaata actgtttcag tttccatctt
      901 aataactgct acttccatct gctttttata gctatcagcc gatccttcga cagcattatt
      961 caataaaacg ctcatgatac gaaccaaatc caatagttca attggaagct tggtaatcgt
     1021 atcttttact tccagtgtaa actctacacc attatttcga gcatagacaa ttgactgagc
     1081 aaccaaactt cgtaaagctg agtcttctat gttgttcaaa tcaaagtaag tgtacttatc
     1141 tgaacgcaat ttatgatttg ctttgactaa aacttcattg taaattctgt caatttcctg
     1201 taaattacca ctgtcaattg ccatctgcat gctgacaagc attccagcat aatcatgtcg
     1261 aaaaccacgg atttcattat acagaccaac aatttcatct gtgtaattct gtaaatgttt
     1321 ctgttcaaat ttcttctgct tcaaagcaat ctctttctcc atttgaactt tatgagaatt
     1381 cattgcaaag aaggtcaaaa ggagagagat aaagacaata gatgacaaaa tacttccaaa
     1441 actattcaaa tgtttaatcg tacttaccat atctgaaacg aaagatacaa tatgtagcaa
     1501 tagtaaagca aaaaatactt ttttcaagaa aggataaagg tagtcctcgt caaaataggc
     1561 tagttccaaa tggaaatagt aaatgatttt taatgtaaca aaataggtta acaccgtcac
     1621 aacgaaaaag aatgggaaat gatattgtaa aacaaaatta tctcctgtta tagaggagaa
     1681 agttacggac agaaagttat gagtgctctc atataaaaga gatagtagta aacttaggaa
     1741 tagtcctcta tccctctcat actgtttcat ccatcgaaaa taggaatata atcccaaagg
     1801 aaataaaaat ctttcaatcc ctaacccatc taaatataga atatataagg gaaaatcaaa
     1861 tactatttct gttagtaatg tataagcacc aaaaacgaat aattcttttc tatttatttg
     1921 acctttacaa ataaaatggt aactgtgact aataattaaa aaatgaacaa taaccgtccc
     1981 aaatccaaat aaatccatta ctctttcccc ttatttcatt acttttttct ttgtaaaata
     2041 aaatcacgga agaattttga caacctcatc tccccacctt taatcttttg taagtctttt
     2101 tccttcaaag ctacaaactg ttccaattta actgtgtttt tcataataaa atctcctaaa
     2161 atgttttttc ttgtaagcta acttacaaaa accattatac aaaatggaat ttcgttttag
     2221 ataaaattct ctcaactgtc atttttttct cccaaagtgt acttttttaa gaaaaaagcc
     2281 gggaaaattc ccagctttgc tattatattg atcccagcag gattcgaacc tgcgaccgtt
     2341 cgcttagaag gcgaatgctc tatccagctg agctatgaga tctaatacaa ttattctacc
     2401 aaaaattcaa ttaaaagtca attttctatt tatggtaggg gaatccctgc tgaatcgtaa
     2461 aagcgcgata gatttgttca acaagaacta gtctcattaa ctgatggggt aaggttaggc
     2521 gaccaaaact gacagaaaga ttggctctat tttttacaga tgatgacaat cctaaacttc
     2581 ccccaataat aaaagtaaga gtagaaaatc cttttataga agtttcttct aactgcttac
     2641 taaattcttc tgagaagaaa gttttccctt caatggctaa cacaataacg aaatcacggt
     2701 cagcaatttt tgataaaatt ctctgacctt ctatttctaa aatcttttga ttttctgatt
     2761 cactggcctt atctggtgtt ttttcatctg ataactcaat catttcaaac ttagcaaatc
     2821 tagaaattcg ttttgaatac tctgcgatac catcttttaa atacttttct ttcagtttcc
     2881 caactgttac aactttaatt ttcatgactc tattctaaca tattctctat tttttcacat
     2941 cttattcaca aaataaaaaa tagatttcaa ttaagaaaat cacagtttca aaagagttat
     3001 ccacagtttg tgtaaaactt ttgtgtttaa gttataatta agctagtcag tttatacttt
     3061 cagtaattca aacatatgga ggcaaatatg aaacatctaa aaacatttta caaaaaatgg
     3121 tttcaattat tagtcgttat cgtcattagc ttttttagtg gagccttggg tagtttttca
     3181 ataactcaac taactcaaaa aagtagtgta aacaactcta acaacaatag tactattaca
     3241 caaactgcct ataagaacga aaattcaaca acacaggctg ttaacaaagt aaaagatgct
     3301 gttgtttctg ttattactta ttcggcaaac agacaaaata gcgtatttgg caatgatgat
     3361 actgacacag attctcagcg aatctctagt gaaggatctg gagttattta taaaaagaat
     3421 gataaagaag cttacatcgt caccaacaat cacgttataa atggcgccag caaagtagat
     3481 attagattgt cagatgggac taaagtacct ggagaaattg tcggagctga cactttctct
     3541 gatattgctg tcgtcaaaat ctcttcagaa aaagtgacaa cagtagctga gtttggtgat
     3601 tctagtaagt taactgtagg agaaactgct attgccatcg gtagcccgtt aggttctgaa
     3661 tatgcaaata ctgtcactca aggtatcgta tccagtctca atagaaatgt atccttaaga
     3721 tcggaagatg gacaagctat ttctacaaaa gccatccaaa ctgatactgc tattaaccca
     3781 ggtaactctg gcggcccact gatcaatatt caagggcagg ttatcggaat tacctcaagt
     3841 aaaattgcta caaatggagg aacatctgta gaaggtcttg gtttcgcaat tcctgcaaat
     3901 gatgctatca atattattga acagttagaa aaaaacggaa aagtgacgcg tccagctttg
     3961 ggaatccaga tggttaattt atctaatgtg agtacaagcg acatcagaag actcaatatt
     4021 ccaagtaatg ttacatctgg tgtagttgtt cgttcggtac aaagtaatat gcctgccaat
     4081 ggtcaccttg aaaaatacga tgtaattaca aaagtagatg acaaagagat tgcttcatca
     4141 acagacttac aaagtgctct ttacaaccat tctatcggag acaccattaa gataacctac
     4201 tatcgtaacg ggaaagaaga aactacctct atcaaactta acaagagttc aggtgattta
     4261 gaatcttgat tgacatctat gtaaagaaag ctttacataa gagaaaagat gtgttagtgt
     4321 agaatcatgg aaaaatttga aatgatttct atcacagata tacaaaaaaa tccctatcaa
     4381 ccccgaaaag aatttgatag agaaaaacta gatgaactag cacagtctat caaagaaaat
     4441 ggggtcattc aaccgattat tgttcgtcaa tctcctgtta ttggttatga aatccttgca
     4501 ggagagagac gctatcgggc ttcactttta gctggtctac ggtctatccc agctgttgtt
     4561 aaacagattt cagaccaaga gatgatggtc cagtccatta ttgaaaattt acagagagaa
     4621 aatttaaacc caataaaaga agcacgcgcc tatgaatctc tcgtagagaa aggattcacc
     4681 catgctgaaa ttgcagataa gatgggcaag tctcgtccat atatcagcaa ctccattcgt
     4741 ttactttcct tgccagaaca gattctttca gaagtagaaa atggcaaact atcacaagcc
     4801 catgcgcgtt ccctagttgg gttaaataag gaacaacaag actatttctt tcaacggatt
     4861 atagaagaag atatttctgt aaggaaatta gaagctcttc tgacagagaa aaaacaaaag
     4921 aaacagcaaa aaactaatca tttcatacaa aatgaagaaa aacagttaag aaaactactc
     4981 ggattagatg tagaaattaa actatctaaa aaagacagtg gaaaaatcat tatttctttt
     5041 tcaaatcaag aagaatatag tagaattatc aacagcctga aataaggctg ttcttttatt
     5101 tttttatctc acaaggttat ccactatgtt tttcgataaa aagcttaata aatcaataat
     5161 ttcttctttt atccccaacc tgtggataaa gtttggtaac attgtggatt atttttcaca
     5221 gcttgtggaa aattcttgct atctatggta aaatatctct agtattaaac ttttaaatag
     5281 taaaggagga gaaaggattg aaagaaaaac aattttggaa tcgtatatta gaatttgcac
     5341 aagaaagact gactcgatcc atgtatgatt tctatgctat tcaagctgaa ctcatcaagg
     5401 tagaggaaaa tgttgccact atatttctac ctcgctctga aatggaaatg gtctgggaaa
     5461 aacaactaaa agatattatt gtagtagctg gttttgaaat ttatgacgct gaaataactc
     5521 cccactatat tttcaccaaa cctcaagata cgactagctc acaagttgaa gaagctacaa
     5581 atttaactct ttataactat agtccaaagt tagtatctat tccttattca gatacgggat
     5641 taaaagaaaa gtataccttt gataacttta ttcaagggga tggaaatgtt tgggctgtat
     5701 cagccgcttt agctgtctct gaagatttgg ctctgaccta taaccctctt tttatctatg
     5761 gaggaccagg ccttggtaag actcacttat taaacgctat tggaaatgaa attctaaaaa
     5821 atattcctaa tgcgcgtgtt aaatatatcc ctgccgaaag ctttattaat gactttcttg
     5881 atcacctaag acttggggaa atggaaaagt ttaaaaagac ctatcgtagt cttgatcttt
     5941 tgttaatcga tgatattcag tcactcagcg gaaaaaaagt tgcaactcag gaagaatttt
     6001 tcaatacctt taacgccctt catgacaagc aaaaacagat tgtcctaacg agtgatcgta
     6061 gtccaaaaca tctagaaggg ctcgaggaga ggcttgtcac gcgttttagt tggggattga
     6121 cacaaactat cacaccccct gactttgaaa cacgtattgc cattttacaa agtaaaacgg
     6181 aacatttagg ctacaatttc caaagtgata ctctagaata cctagctggg caatttgatt
     6241 caaatgttcg agatcttgag ggagccatca acgacatcac tttaattgcc agagtaaaaa
     6301 aaatcaagga tatcactatt gatattgctg cagaagccat tagagcccgc aaacaagatg
     6361 ttagccaaat gctcgtcatc ccaattgata aaatccaaac tgaagttggt aacttttatg
     6421 gtgttagtat caaagaaatg aagggaagta gacgccttca aaatattgtt ttggcccgtc
     6481 aagtagccat gtatttatct agagaactaa cagataatag tcttccaaaa attgggaagg
     6541 aatttggggg aaaagatcat accacagtca ttcatgccca tgccaaaata aaatctttga
     6601 ttgatcaaga cgataattta cgtttagaaa ttgaatcaat caaaaagaaa atcaaataat
     6661 ttgtggataa cttttagttt tttatctttt ttatccacat tttttaaaca agctaaaaaa
     6721 cttgatatga cttgtttaaa ggctgttttc caca
//
LOCUS       DAVKWS010000079         6342 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKWS010000079 DAVKWS010000000
VERSION     DAVKWS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.080"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            1..291
                     /locus_tag="VW852_001906"
                     /pseudo
     CDS             1..291
                     /locus_tag="VW852_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            446..646
                     /locus_tag="VW852_001907"
     CDS             446..646
                     /locus_tag="VW852_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272987.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            671..1087
                     /locus_tag="VW852_001908"
     CDS             671..1087
                     /locus_tag="VW852_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272988.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRHKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1318..2025
                     /gene="ccdA2"
                     /locus_tag="VW852_001909"
     CDS             1318..2025
                     /gene="ccdA2"
                     /locus_tag="VW852_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0272989.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2036..2593
                     /gene="sdbB"
                     /locus_tag="VW852_001910"
     CDS             2036..2593
                     /gene="sdbB"
                     /locus_tag="VW852_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV0272990.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGQEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2967..4358
                     /locus_tag="VW852_001911"
     CDS             2967..4358
                     /locus_tag="VW852_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV0272991.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4501..5418
                     /gene="adcAII"
                     /locus_tag="VW852_001912"
     CDS             4501..5418
                     /gene="adcAII"
                     /locus_tag="VW852_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV0272992.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            5426..>6342
                     /gene="phtB"
                     /locus_tag="VW852_001913"
     CDS             5426..>6342
                     /gene="phtB"
                     /locus_tag="VW852_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV0272993.1"
                     /translation="MKINKKYVAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRT"
BASE COUNT         1945 a         1138 c         1338 g         1921 t
ORIGIN      
        1 aattttaata gtttaaagca cctagcacct gtttagattg atgaaacagg attcgatact
       61 tatttttatc gagaatatgg tcgctcatta aaaggtcagt taagaagagg caaagtatct
      121 ggaagaagat atcagaggat ttctttggtt gcaggtctaa caaatggtga attaatcgct
      181 ccaatgactt acgaagagac gatgacgagc gacttttttg aagcttggtt tcagaaattt
      241 ctcttaccaa cattaaccac accatcggtt attatattaa aacgaaataa gaataggaca
      301 aatcgatcag gacagtaaaa tcgaattcta acaatgtttt agaagtccat atgtactatt
      361 ctagtttcaa tataccataa ctgctcctag tcaacctgca agtcctgtcc cagatccaag
      421 ctcagctccg actcaagatc ctagagtggt agtaagcgct tcggctagct caacgagccc
      481 acaagctcaa gagcaagttg acaagtctga acttcgtgcc ttgagtcaag agttagacca
      541 acacttgaaa gctttggcaa cagtatctga tccgaaaatc gatgcaacca aggctgtcct
      601 cctagatgct caaaaagctc cggaagatag tgccttgaca gagtaaggtc tgcatactgc
      661 agtagagtct gtgaaggctg ccttggactc tctcaaagat gtgaaagcaa atgctagcga
      721 cagcaagcct gcacaggaca agaaggatgc aaaacaagga acggaagata gtaaggattc
      781 agataagatg actgaaacaa actcagttcc ggcaggagtg attgtggtca gtctacttgc
      841 cctcctaggc gtgattgcct tctggctgat tcgccataag aaagagtcag aaatccagca
      901 attaagcacg gaattaatca aggttctagg acagctagat gcagaaaaag cggataaaaa
      961 agtccttgcc aaagcccaaa accttctcca agaaaccctt gatttcgtga aagaagaaaa
     1021 tggctcagca gagacagaaa ctaaactagt agaggagctt aaagcaatcc ttgacaaact
     1081 caagtaaaac atgatttatg aaatgtcaga cttaaaagac attttcgttc acaaaagcag
     1141 cgacgagtaa tccttgtcgt tgctttttgg ctattttggg ggataagatc cgccttgact
     1201 agcaaaagaa aagattggtc caatagtgcc cgatacgatt ttgcgtagca gaaagcttac
     1261 aaataaaatt gaatatagta aaataggatt agaattatta agaaagttgg ttctttattg
     1321 gaaacgatag tatttttaat ctctgttttt ctagcaggtg ttttatcctt tttttctcct
     1381 tgtatttttc ctcttctgcc agtctatgct gggattttat tggatgatca agaaagtgca
     1441 aaaagctttt ctttgtttgg gagaaaggtt ctctggtcag gcttgattcg aacactttgc
     1501 tttatcgctg gtatctctct cattttcttt attctaggct ttggtgctgg ttactttggt
     1561 catattctct atgcaaattg gtttcgatat ggcatgggag ctattattat cattttgggt
     1621 cttcaccaga tggaaatttt tcatttgaag aaattagaag ttcaaaaaag ttttaccttt
     1681 aaaaaatcag attctaatcg ttattggtca gcttttttac ttggtattac ctttagcttt
     1741 ggttggacac cttgtattgg tccagtttta agttctgttt tagcacttgc ggcttctgga
     1801 ggcaatggcg cttggcaagg agcgatttat actctcattt acactctggg catggccctt
     1861 cctttcttgg tattggcact agcttcaggt ctagtcatgc catattttag taaaatcaag
     1921 cgtcatatga tgctactaaa gaaaattggt ggtttcctca ttgttttaat gggaattttg
     1981 ttactattag gacaagtaaa tgttctagct ggaatttttg aataaaggag gacaaatgaa
     2041 aaaagtaatg tttgctggct taagtctctt gtcattagtt gtattgatgg cctgtggtca
     2101 ggaagaaact aaaaagactc aagcagcaca acagccaaaa caacaaacga ctgtacaaca
     2161 aatttctgtt ggaaaagatg ctccagactt tacattgcaa tccatggatg gcaaagaagt
     2221 taagttatct gattttaagg gtaaaaaggt ttacttgaag ttttgggctt catggtgtgg
     2281 tccatgcaag aaaagtatgc cagagttgat ggaactagcg gcgaaaccag atcgtgattt
     2341 cgaaattctt actgtcattg caccaggaat tcaaggtgaa aaaactgttg agcaattccc
     2401 acaatggttc caggaacaag gatataagga tatcccagtt ctttatgata ccaaagcaac
     2461 caccttccaa gcttatcaaa ttcgaagcat tcctacagaa tatttaattg atagccaagg
     2521 aaagattgga aagattcaat ttggtgctat cagtaatgcg gatgcagaag cagcatttaa
     2581 agaaatgaac tagaatcgat aaaatctgat tacaagatgt agtcagattt tggtaacttc
     2641 taaaactaac ttccagtttc ccacaactta gcttttagta tgagaaaaac gcgtgacatc
     2701 agtggaaatc cgtcttagac taactttcac tggttttctt tttcttgata tataagggtt
     2761 caaaagcgaa aagactcaga aaaagtgcac gacaaatagc cctaaaacag agtcgtctta
     2821 gagtaacttc cagttttggg agagagatgg aagttacttt gagaagttac ggtagtcaga
     2881 tttttttaga aaaacatttt ataaatattc acatatctcc tatatttatg gtaaaataga
     2941 attatcagtt tattttggag tcaaagatga atatatttag aacaaagaat gttagtttag
     3001 ataaaacaga gatgcatagg catttgaagt tatgggattt gattttgctg ggtatcggag
     3061 ccatggtagg gacaggcgtc tttacaatca caggtactgc agctgcaaca cttgctggcc
     3121 cagccctagt gatttcaatc gttatttctg ccttgtgtgt gggattatca gccctctttt
     3181 ttgcagaatt tgcctcgcga gtacccgcta caggaggtgc ctatagttac ctctatgcta
     3241 tcttaggaga attccctgcc tggttggctg gttggttaac catgatggag ttcatgacag
     3301 ccatatcagg cgtagcttcg ggttgggcag cttattttaa aggcttgctc tctcaatacg
     3361 ggatagcctt tcctcaggct ttaaatggta cctttaatcc tcaagcaggg acatttgttg
     3421 atttattgcc tattcttgtc ttggtcttgg tgacttcgct tgttttacta aatgccaagg
     3481 cagccttacg ctttaattcg attctagtta ttttgaaatt ttccgcttta gctctctttg
     3541 ttttagtagg aatttggaat attaagtttg ataattggag caattttgct ccttacggtt
     3601 ttggacaaat ctatggagct agcactggta ttatggctgg tgcgtccttg atgttcttcg
     3661 gttttttggg atttgaatcc atctctatgg ctgtagatga agtcaagact cctcaaaaaa
     3721 atattcctag agggattgtc ttatcgcttt ctatcgtaac cattctctat gccttggtga
     3781 cccttgtttt gactggtgtg gttcactata gtcatttaaa tgtcgatgat gccgttgcct
     3841 ttgcccttcg tagtgttggg attagttggg cagccaacta tgtgtcatta gtggctatct
     3901 tgaccttgat tacagtttgt atttcgatga cctatgccct atcgcgtatg atttacagtt
     3961 tagcacgtga cggcttagtg cctgctgcct ttaaggaact gacgaagact agcaagatac
     4021 caaagaatgc tactatcttg acaggtctag cttcagcagt agcagcagga atgttcccac
     4081 tagccagtat tgcagccttt ttaaatattt gtaccttagc ctacttgatc atgttggctt
     4141 atggtctgat tcgcttacgg aaagaaaaag gaatgcccaa agctggggaa tttaaaacac
     4201 cattggtacc tctgttacca attttatcga tcatcatttg tttatccttc atgttacagt
     4261 ataatatgaa cacctggatt gctttcctag ttgcgttgtt agtaggaagt attatttact
     4321 tcacttatgg ctataagcat tctaccatag aagaataaag tgaaagtcta ttgagttgtt
     4381 gacactcagt agatttttat atgtgaaaga aatttcaaat tttttctaaa aatatcttga
     4441 cagattaaat tttcaggagt agaatattta ctagttaatt aaatggttaa ggagttgttc
     4501 atgaagaaac aaaatttatt tttagtcctg ttaagtgtct ttcttttgtg cttgggggct
     4561 tgtggtcaaa aggaaagtca gacaggaaag gggatgaaaa ttgtgaccag tttttatcct
     4621 atctacgcta tggttaagga agtatctggt gacttgaatg atgttcggat gattcagtca
     4681 agtagtggga ttcactcctt tgaaccttcg gcaaatgata tcgcagccat ctatgatgca
     4741 gatgtctttg tttaccattc tcatacactc gaatcttggg caggaagtct ggatccaaat
     4801 ctaaaaaaat ccaaagtgaa ggtcttagag gcttctgagg gaatgacctt ggaacgtgtc
     4861 cctggactag aggatgtgga agcaggggat ggagttgatg aaaaaacgct ctatgaccct
     4921 cacacatggc tagatcctga aaaagctgga gaagaagccc aaattatcgc tgataaactt
     4981 tcagaggtgg atagtgagca taaagagact tatcaaaaaa atgcgcaagc ctttatcaaa
     5041 aaagctcagg aattgactaa gaaattccaa ccaaaatttg aaaaagcgac tcagaaaaca
     5101 tttgtaacac aacatacagc cttttcttat ctagcgaaga gatttgggct taatcaactt
     5161 ggtattgcag gtatctctcc tgaacaagaa ccaagtccac gacaactaac agaaattcag
     5221 gaatttgtta aaacctataa ggttaaaacg atttttacag aaagtaatgc ttcttcaaaa
     5281 gtagctgaaa ctcttgtcaa atcaacaggt gtgggtctta aaactctgaa tcctttagag
     5341 gcagacccac aaaatgacaa gacctattta gaaaatctgg aagaaaatat gagtgttcta
     5401 gcagaagaat taaagtgagg aaagaatgaa aatcaataaa aaatatgtag caggttcagt
     5461 ggcagttctt gccctaagtg tttgttccta tgaacttggg cgttaccaag ctggtcagga
     5521 taagaaagag tctaatcgag ttgcttatat agatggtgat caggctggtc aaaaggcaga
     5581 aaatttgaca ccagatgaag tcagtaagag ggaggggatc aacgccgaac aaattgttat
     5641 caagattacg gatcaaggtt atgtgacctc tcatggagac cattatcatt actataatgg
     5701 caaggttcct tatgatgcca tcatcagtga agagctcctc atgaaagatc cgaattatca
     5761 gttgaaggat tcagacattg tcaatgaaat caagggtggt tatgtcatta aggtaaacgg
     5821 taaatactat gtttacctta aggatgcagc tcatgcggat aatattcgga caaaagaaga
     5881 gattaaacgt cagaagcagg aacgcagtca taatcataac tcaagagcag ataatgctgt
     5941 tgctgcagcc agagcccaag gacgctacac cacagatgat ggttatatct ttaatgcttc
     6001 tgatatcatt gaagatactg gtgatgccta tatcgttcct catggcaatc acttccacta
     6061 tattcctaag agtgatttat ctgccagtga attagctgct gcccaagcct attggaatgg
     6121 gaagcaggga tctcgtcctt cttcaagttc tagtcataat gcaaatccag ctcaaccaag
     6181 attgtcagag aaccacaatc tgactgtcac tccaacttat catcaaaatc aaggggaaaa
     6241 catttcaagc cttttacgtg aattgtatgc taaaccctta tcagaacgcc atgtggaatc
     6301 tgatggcctt attttcgacc cagcgcaaat cacaagtcga ac
//
LOCUS       DAVKWS010000080         6295 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKWS010000080 DAVKWS010000000
VERSION     DAVKWS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.081"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            115..723
                     /locus_tag="VW852_001914"
     CDS             115..723
                     /locus_tag="VW852_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0272994.1"
                     /translation="MPKTIPYDILKNIFSYLEQRVVISKTDYQKQKAERNLLIISLLL
                     STGIRISELCHIHLKDINLSNKTLHIIGKGKKERILFLGDQTTFNLLETYINKNGKES
                     NDFLFPGKHSPKPLSEQSVRLILKRIVEQNGLSKTITPHMFRHSFATMLLDNDVDIRY
                     IQQILGHSSISITQIYTHVSHSKQKEILSSFNPMSVIHSEIE"
     gene            complement(1101..1481)
                     /locus_tag="VW852_001915"
     CDS             complement(1101..1481)
                     /locus_tag="VW852_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV0272995.1"
                     /translation="MKSMGYEVQLALIATKPKLSYLSTLIRYEKLYAINPDQARATPK
                     EHHDFIVNHLVDNTRQLEKLAIFERIQIYQRDRSCVYDSEEDKTSVATVLHDLLFGEW
                     NQVEKEMLKSGEGKLKDLTNRNGC"
     gene            1506..1682
                     /locus_tag="VW852_001916"
                     /pseudo
     CDS             1506..1682
                     /locus_tag="VW852_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1841..2068)
                     /locus_tag="VW852_001917"
     CDS             complement(1841..2068)
                     /locus_tag="VW852_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272996.1"
                     /translation="MAVVSLENNIKLYSSELFQALLKASNYKLDKRIAQTVAEGYARN
                     LDYSDPELMHVGVTSVANNLLTKIKQEYFNV"
     gene            complement(2059..2691)
                     /locus_tag="VW852_001918"
     CDS             complement(2059..2691)
                     /locus_tag="VW852_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HEV0272997.1"
                     /translation="MELTTKQEKQLGQTQWFHATLLRHLESLKSGIDVKFNLGSELDF
                     GPGFYITPDFEQARKFINKQVEVLNRSSSNNNIFDSEEEVGIIVEFRISNFIEIFKNP
                     DYHCHYFAKHKKSESDLDFAEFVVQNRENPDELQHHFDFIYGVQTDDNPTQALARFRQ
                     NEITKEEMLAEFRKPYSFKQLSIHNQSFCDIMKIEKVYQSKTGEELQKWQ"
     gene            complement(2773..3048)
                     /locus_tag="VW852_001919"
     CDS             complement(2773..3048)
                     /locus_tag="VW852_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0272998.1"
                     /translation="MFTTFFKKNHDNSDVFKKLIHRLSDMSVQDLEKIDRLLDIIFTP
                     DQESEQVKTESIYREETLDDTLKEAKNQLHKEQLEKNLERFRKDGRK"
     gene            complement(3086..3469)
                     /locus_tag="VW852_001920"
     CDS             complement(3086..3469)
                     /locus_tag="VW852_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEV0272999.1"
                     /translation="MTKDWNFNQPLESKSENQEDPDKIAALFRNHQGGNDVNYEAAFQ
                     KRKQAPVTDSNSSSKPKVTEVRTGKETDITTSYQQHLKRLIADNNSDIQSSQKKIEEL
                     HTFIDTKNKDNKKLQSIYDAISDLH"
     gene            complement(3466..3699)
                     /locus_tag="VW852_001921"
     CDS             complement(3466..3699)
                     /locus_tag="VW852_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273000.1"
                     /translation="MTIIERLEEKVTRQESKVARETEKLAAYKEQLETAMFATFKRRQ
                     SISHMSFEEALDHAFGKERQFDDSEFRKDEMSE"
     gene            complement(3750..4835)
                     /locus_tag="VW852_001922"
     CDS             complement(3750..4835)
                     /locus_tag="VW852_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEV0273001.1"
                     /translation="MEIEECKKISILDVASRLGISFKQVSSSVYEHPEHDSFRIFSTT
                     NTFKWFSRDIQGDVIDFVRLVKGISFKEALAFLSEEPFQKETVQEKRERPFYYPLKRI
                     EDSHCSLARYYLTECRGISEEIVQKMIQQGLIVQASWKTNETVEPVIVFKSFNHRHKL
                     QAASLQGIYKNHSLPRERLKTILKGSHGHVGISFDIGKPNRLVFCESFIDLMSYYELH
                     QQSLTDVRLVSMEGLKRSVVAYQTLRLIAEENQRLEFLDTVIPSKLLPLINTIRDTTS
                     YFDNHPDLLTLAVDCDDAGKDFSDKLSQSGFPVFLDFPDNKSGKEKVDWNDVLREKKS
                     DLQLMIENAKETLRNQPVRKTSQCLEL"
     gene            complement(4845..5486)
                     /locus_tag="VW852_001923"
     CDS             complement(4845..5486)
                     /locus_tag="VW852_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV0273002.1"
                     /translation="MLNKVKTKALISVGAVAATSFILMMGYTAGQHSTAKQSRKEIEL
                     AAAKLVEDKQAEDKASILLSDTVKEFLTQYYTKEKLGENNTRIQPYMTESAYSQELSS
                     QNDAMNQVYKDYILDYHFEKADIFVNQTTNQAIAMVSYNVTYVSDLKNANQSKTNQTE
                     TRTVKLSYSKLPGKLLVNQVQVWKSGLDDLDKATPKTLEESSSVPSLPNTTTK"
BASE COUNT         2036 a         1143 c          901 g         2215 t
ORIGIN      
        1 ttaagaagaa aaattgcttg tataaaggtg ttttataact atctaaaata ccagcacata
       61 attgaagaga atcccttcaa tcaattacgc tttcaattta gaactgaaaa aatattgcct
      121 aaaacgattc cgtatgatat tctgaaaaat attttttcat atttagagca gagagtagtt
      181 atatctaaaa ctgactatca aaaacaaaaa gctgaaagaa atctactaat tatttcccta
      241 ttactttcaa caggtatcag aatttctgaa ctttgtcaca ttcatctcaa agatattaat
      301 ctttccaata agacactcca tattatagga aaaggtaaga aagagcgtat cctattttta
      361 ggagatcaaa caaccttcaa tttattagaa acatatataa ataaaaacgg aaaggaatct
      421 aatgatttcc tattcccagg aaaacattcg cctaaaccat tgtcagagca aagtgtacgt
      481 ttaattttaa agagaatcgt tgaacaaaat ggcttatcta aaactattac accacatatg
      541 tttagacata gctttgcaac aatgcttcta gataatgatg tagatattcg atatattcaa
      601 caaattcttg gacacagttc tatatcaatc acacaaatct atactcacgt atctcattca
      661 aaacaaaaag aaatacttag ttctttcaat cctatgtcag tgattcattc tgaaatcgag
      721 taagagacat ttccgaaggt tacagtttac ttcatcttaa attaatgttt gtagttgact
      781 ttagttaata gattttatac tcaacaaaag caaaaagcat gcaaaactcc actttgaatc
      841 agcatttata ctatattccg tattaagaac tccataattg tttttctaaa atataacttt
      901 caagtatcct taaatataat gaaaagagat gggcaaaatc tcaattcctg aagaaaatgg
      961 agtaaatctt cccacaagaa aacgcataat ttcaagtttt tcaacacctg agactatgcg
     1021 ttttttgctt ctaaaaaagt ttcgcccacc ttaaacaact aacaaaagtt ataagtagac
     1081 tatattttaa aactcaagat ctaacaacca tttcgattag ttaaatcttt caattttcct
     1141 tctcctgatt taagcatctc tttctcaact tgattccatt cgccaaatag taaatcatga
     1201 agaacggttg ctactgaagt tttatcttct tctgaatcgt atacacaact tctatctcgt
     1261 tggtaaattt gaattctttc aaagatagct agtttttcca attgtcttgt attatcaact
     1321 agatgattta caatgaaatc atgatgttct tttggagttg cgcgtgcttg atctggattg
     1381 atagcataca gtttttcata acggataagg gtgctcagat aggacagctt aggctttgtc
     1441 gcaatcaaag ctaattgtac ttcatatccc atacttttca agagttgtgc tgtttccttt
     1501 ggaacatcaa cgccctttaa ctaccctatt ctaacgccaa attggaagcg actaataatc
     1561 tcattaaact catcaaacga aatgcctttg gttttcgaaa ctttgaaaac ttcaaaaaac
     1621 ggatttttat cgctctgaac atcaaaaaag aaaggacgaa atgtgtcctt tcaagatctt
     1681 agcttttctt caacccactg cagttgacaa agagccgtaa catttagaaa aaagaaatgc
     1741 accatttttg gtgcgttact tcttgttttt ctagcctaaa ctttacttct aaaaaagcca
     1801 ccttaaagag taattccttt ttcggtggct ttttaaaata ctatacatta aaatactctt
     1861 gtttaatttt tgtaagcaga ttgttagcaa ccgaagttac accaacatgc atcagttcag
     1921 gatcagaata atctagatta cgagcatagc cttctgcaac agtctgagca atacgcttat
     1981 ccaatttata attagaggct tttaatagtg cttgaaataa ttctgaacta tatagtttaa
     2041 tattattttc caatgatact actgccattt ttgtaactcc tctcctgttt ttgattgata
     2101 aaccttctct attttcatta tatcacaaaa agattggttg tggatggata attgtttaaa
     2161 agaatatggt tttctaaact cagcaagcat ttcttctttt gtaatttcat tctgacgaaa
     2221 acgagctaaa gcttgggttg gattgtcatc agtctgaaca ccatagataa aatcaaaatg
     2281 gtgttgtaat tcatctggat tttcccgatt ttgaacgaca aattcagcaa aatctaaatc
     2341 tgactctgac tttttatgtt ttgcaaaata gtgacaatga tagtcagggt tcttaaaaat
     2401 ttctataaaa tttgaaatac gaaattctac tataattcct acttcttctt ctgagtcaaa
     2461 aatattatta ttagagctgc ttcgatttaa tacttcaact tgcttgttaa taaattttct
     2521 agcttgttca aagtcaggcg tgatatagaa accaggacca aaatctagct cacttcctaa
     2581 attaaattta acatctatac cactttttaa ggattctaaa tgtctcaata atgtagcatg
     2641 aaaccactgc gtctgaccta gttgcttttc ctgcttggta gtcaactcca aaatatttac
     2701 ttccttctct agtctatact aaaagtataa cacaagactt tattcaagta aaagaaaatt
     2761 gtataaaact attcacttcc ttccgtcttt cctaaatctc tctaaattct tctccaattg
     2821 ttccttatga agttgattct tagcttcctt taatgtgtcg tccaaagttt cttccctata
     2881 aattgattct gtttttactt gttctgattc ttgatctgga gtgaaaatga tatctaacag
     2941 tcggtctatt ttttcaagat cttggacaga catatcagat agtctatgaa tcagcttttt
     3001 aaatacatca ctattgtcgt gatttttctt aaaaaatgtt gtaaacatct ataacctctc
     3061 aaaaaaagca gcctatcagg actgcttagt gtaaatccga aatcgcatca taaatggact
     3121 gtaatttctt attgtcttta ttctttgtgt caataaatgt atgtagctct tcaattttct
     3181 tttgactgct ttgaatatcg ctattgttat ccgcaataag tcttttaaga tgttgctgat
     3241 aacttgtagt gatatctgtc tcttttcctg ttctaacctc tgtaactttg ggtttagaac
     3301 tagaatttga atccgtcaca ggtgcttgtt ttcgcttttg aaaagctgct tcataattta
     3361 catcattacc tccttgatga tttctaaata aggccgcaat tttatctgga tcttcttgat
     3421 tttctgattt actttctaat ggttgattaa aattccaatc ttttgtcatt cactcatttc
     3481 atcctttcta aattcagaat catcgaattg tctttcttta ccaaaggcat ggtcaagagc
     3541 ttcttcaaaa ctcatgtgac taatgctttg acgccttttg aacgtcgcaa acatcgctgt
     3601 ctccagttgt tctttgtaag cagcgagttt ttctgtctct cttgctacct tactttcttg
     3661 cctagtgacc ttttcttcta agcgttcaat aatggtcata tacgatcctc cttcattcta
     3721 atattagcta aaacatcttg attttgttat cacaattcta aacattgaga ggtttttctt
     3781 actggttgat tcctcaatgt ctcttttgca ttctcgatca tcaattgtaa atctgatttc
     3841 ttttctctaa ggacatcatt ccagtctact ttttctttcc cagatttatt atcagggaaa
     3901 tccagaaaaa caggaaatcc tgattgagat aacttatcag aaaaatcttt tcctgcatca
     3961 tcacaatcta ccgcaagtgt caataaatca ggatgattat caaaatagct ggtggtatca
     4021 cgaattgtat tgattaaagg caataacttt gaaggtatta ctgtatccaa aaattccaac
     4081 ctctgatttt cttcagctat cagtcgtaaa gtttgataag caacaacaga cctttttaat
     4141 ccttccatag ataccaaacg aacatcagtt agactttgtt gatgaagttc gtaatagctc
     4201 attaagtcga tgaacgattc acaaaagacc agtctattag gtttaccaat atcaaaggat
     4261 attccaacat gtccatggct tccttttaga atcgttttta acctctctct aggaagagag
     4321 tgattcttat aaatcccttg gagacttgct gcctgcagct tgtgacgatg attaaagctt
     4381 ttaaaaacaa taacaggttc aactgtttca tttgttttcc aactagcttg tactatcaaa
     4441 ccttgttgaa tcatcttttg tacgatttct tctgaaattc ctctacattc tgttaagtaa
     4501 tatctggcca gactacagtg agaatcttct attctcttta aaggataata aaatggtctc
     4561 tctctttttt cttgaacagt ttctttttga aaaggttctt cagaaagaaa ggctagagct
     4621 tctttaaagg aaattccctt aacaagtcga acaaaatcaa tgacatcacc ttgaatatct
     4681 cttgaaaacc atttaaaagt attggtagtt gaaaaaatcc gaaatgaatc gtgttcagga
     4741 tgttcataga cactgctcga aacttgttta aaggagatac ctaaacgact tgctacatca
     4801 agaattgaaa ttttcttaca ttcttctatt tccatgcaat atcatcattt tgtcgtagta
     4861 tttggcagtg atggaacaga tgaggattct tctaaagttt taggagttgc cttatctaaa
     4921 tcatctaacc cagatttcca aacctgtact tggttgacca ataacttacc aggtagttta
     4981 gaataggata acttaactgt tcttgtttct gtctgattcg tctttgattg gttggcattc
     5041 tttaaatcag atacataggt tacattataa gagaccatgg caatagcttg attcgtagtc
     5101 tgattgacaa agatatcagc tttttcgaaa tgataatcta aaatataatc cttatacact
     5161 tggttcatgg catcattttg acttgacaat tcttgagaat aagccgattc agtcatataa
     5221 ggttgaatac gtgtattatt ttctccgagc ttttctttcg tatagtactg tgtcaaaaat
     5281 tcttttacag tatctgataa caaaatactt gctttatctt ctgcttgttt gtcctctaca
     5341 agtttagctg cagccaattc aatctcttta cgactttgtt tagcagtaga atgttgacca
     5401 gcagtatatc ccatcatgag aataaagcta gttgcagcca ctgctccaac actaattaag
     5461 gctttagttt tgactttatt taacatctta gaccttcttt ctataaaaac taggtcaaga
     5521 ataacaaaaa gaacttgtaa gttttatcac atcacaacga aaggtaattc ctacatttca
     5581 ggcacaaaat cgtacagttc agagaaaaaa aaaactagct gtctttgaca gtataataga
     5641 cttattggaa aaaaataaaa tgcatactgt actatgctat actatagtta agaaaactat
     5701 ttacaaaaga tcatgcaact aacttctgag aactttaaat tatttattag tgtaaaaaag
     5761 gacaccttcc caacgaagat atcctgtcta aataaagctt ataaaaccaa acactataaa
     5821 ggaggacgtt caaaaacgct aagtctataa aaacagttga tgctaaactt gcgttattta
     5881 ttctattgaa caactctacg tctcctagct tttgattttg gtcttggatt agcgacagtc
     5941 accgaacgat aatattggta gaagatactc ttcgttcttc aggtatattt gccttagaca
     6001 acttaaaagc agcaagtgtc actatagcta ttgatgtcat agaaagtcct atttcaatgt
     6061 cctaaagaaa ccaaagtaag aattatcctg gaaaaagata tacaccataa agacacaatc
     6121 atactcgatt taacgacact tcgtgcctgt caattttttt tttgagatgg tcatacacat
     6181 gattttactc tttttaaaga aagtataggt caagtttgtc agagcaatca cttgtttttg
     6241 ttatttagat actgtgaata ttcatcagat atctttattc ctataaaaag ttcaa
//
LOCUS       DAVKWS010000081         6210 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKWS010000081 DAVKWS010000000
VERSION     DAVKWS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.082"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            161..475
                     /locus_tag="VW852_001924"
                     /pseudo
     CDS             161..475
                     /locus_tag="VW852_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            655..1314
                     /locus_tag="VW852_001925"
     CDS             655..1314
                     /locus_tag="VW852_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV0273003.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1339..2406
                     /locus_tag="VW852_001926"
     CDS             1339..2406
                     /locus_tag="VW852_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0273004.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDAKEMPKDWT
                     ELASKYKGQYSIYGLTGGTGRAIYASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESTAVKVLDKENAIQYGMIWGSGALVGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            2510..3520
                     /locus_tag="VW852_001927"
     CDS             2510..3520
                     /locus_tag="VW852_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0273005.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3532..5223
                     /locus_tag="VW852_001928"
     CDS             3532..5223
                     /locus_tag="VW852_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0273006.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5226..5936
                     /locus_tag="VW852_001929"
     CDS             5226..5936
                     /locus_tag="VW852_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV0273007.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EVYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            6032..>6210
                     /locus_tag="VW852_001930"
     CDS             6032..>6210
                     /locus_tag="VW852_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273008.1"
                     /translation="MKDHKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINS"
BASE COUNT         2076 a          864 c         1181 g         2089 t
ORIGIN      
        1 tattatcgct taccaatatt tgcttttata tttgctaaac tttcagaaaa aaagttggag
       61 tatatgatgc taggtgactg cgtcatgtta gtaaacgaaa tggaaataac tgatcataga
      121 gtagataact tatttgaaaa agggaaaaat gagataaaag atccatgtag tagatttagc
      181 aataggacat ataaaagctt tagaaaaagt atctgaaaaa acagatgttt atatctataa
      241 cctaggctcg ggagaaggca ctagtgtatt acaacttgta aatacatttg aaagtgttaa
      301 taagatccct attccttata aaatagttcc aagacgttca ggagacgttg caacttgtta
      361 tgcaaatgca gacaaagcat ataaggaatt aaattggagg acaacaaaat cgattgaaga
      421 catgtgtaga gatacatgga attggcaatc aaaaaatccc aatggctata attgatgata
      481 ctgtctatat gagtgctgta ttttgaaaaa attacttaac cttttaatga aatgcatatt
      541 taatgaaaga taaatttaaa aaaatgtttg caagcgcttg acaaaggaaa cggtttcatt
      601 gtataatgta aatacgatga caccgatgtc aaatataaaa aagaaggtct ttatatgaaa
      661 aacttaacaa aaattaagtt caaagagaat ggggaattta atcatttccc tgggaataca
      721 gttgtagcaa atctttatac taaacaagat ttgatggaag ttgttgatat tattcaatca
      781 cgttatagag aattaccatt tatcgataag tttactttaa ctccaaggaa ttctattcac
      841 atgactgtaa ttgaattgtt gtgccatgaa aatcgtgaaa cggagttttg gagcagtaat
      901 cttcctctag atacaccttt acaggaaata catgattact ttgctaaaca acttgaaatt
      961 tttccattgt tggatgaaga aattcatatg cgtataactg aaatgggaaa acaaaacata
     1021 ctagttgaac ctgcagatga agcttctgca aagagattag aagaaattcg tacttatgtc
     1081 tcagaaaaag caggtgtttg tttccctaat catgatagat atcaattcca tatttcaatt
     1141 gggtatcttc ggattcctct aaccgaagag gaggaagaag agtttactaa agtcagagca
     1201 gaattaactg aaattttatt agagaagatt ccaactatta ctgtaaaccg tattgactat
     1261 actgtatttg aagatatgag acaatttgtt ccatatcacg aaaaatttaa ataaaataat
     1321 aaaaaaggag aacttatcat gaaaaaaatg aaagtttggt ctactgtact tgcaacggga
     1381 gttgctctta ctacacttgc tgcttgctct ggaggttcaa attctacgac tgcttcttca
     1441 tctgaagaaa aagctgataa aagtcaagaa ttagtaatct attcgaactc agtctcaaat
     1501 ggtcgtggtg attggttaac tgctaaagca aaagaagctg gttttaatat aaaaatggtt
     1561 gatattgctg gcgctcaatt agcagaccgt gttattgctg agaagaataa tgcagttgca
     1621 gatatggtat ttggaattgg tgctgttgac tcaaataaaa ttagagatca aaaattacta
     1681 gtacagtaca agcctaaatg gttagataaa attgatcaat ctttatcaga taaagataat
     1741 tattataacc cagttattgt tcaacccttg gtattgattg gagctcctga tgcaaaagaa
     1801 atgccaaaag actggacaga gttagctagt aaatataagg gacaatattc catttatggc
     1861 ttaactggtg ggactggacg cgccatttac gcaagtattc tagttcgtta tcttgatgaa
     1921 aaaggtgatt taggagtatc ggaaaagggc tgggaagttg caaaagaata ctttgcaaat
     1981 gcctatacac tacaaaaagg tgaaagcaca gctgttaaag tattagacaa agaaaacgct
     2041 atccaatacg gtatgatctg gggttctgga gcgttagtcg gacaaaaaga acaaaatgtt
     2101 gaattcaaag ttatgactcc tgagattggt gtaccatttg taactgaaca aactatggtt
     2161 ttaagcacta gtaaaaaaca agcgttagct aaagaattta ttgattggtt tggtcaatca
     2221 gaaattcaag tagaatatag caaaaaattt ggctctatcc ctgccaataa agatgcccta
     2281 aaagagttac ctgaagatac gaaaaaattt gttgatcaag tgaaaccaca aaatattgac
     2341 tgggaagctg ttggaaagca tttggatgaa tgggtagaaa aagctgaatt agaatacgta
     2401 aaataagaaa taagagttat tttttatgtc caagtctctc tattatttct aaatttaagg
     2461 aagaaataaa gagaggcttt tggttttagt aatagaggga gttagtgaga tgattaaatt
     2521 tgataatatt caaattaaat atggtgattt tgttgcaatt gataatctga atttagatat
     2581 acatgaaggg gaattcttta catttcttgg gccttcagga tgtggtaaat caactacttt
     2641 gagagcattg gtaggttttt tagatccatc atcaggaagt attgaagtta atggaacaga
     2701 tgtcactcat ttggaacctg aaaagcgtgg aattggtatt gtatttcaat cttatgcgct
     2761 atttccaact atgactgttt ttgataatat tgcatttggt ttaaaagtta agaaggtagc
     2821 tccagatgtt attaaagcta aagtatcagc agtggcagca aaaattaaga tctctgatca
     2881 acagttacag cgtaatgtat cagaattatc tgggggtcaa caacaaaggg tagcattggc
     2941 tcgtgctctg gttcttgaac ctaaaattct ttgtctagat gaaccattgt caaaccttga
     3001 cgcaaaatta cgtgtagatt tgagaaaaga gttgaaaaga cttcaaaaag agttaggtat
     3061 tactacttta tatgttactc atgatcaaga ggaagccttg actttatctg atagaattgc
     3121 agtctttaac aatggataca tcgaacaggt cggtacacca gtagagattt atcataattc
     3181 tcaaactgaa tttgtatgtg attttattgg agatattaat gttttgaccg atgaaacagt
     3241 ccacgaagta ttattgaaaa atacaagcgt tttcttagag gataaaaaag gatacattcg
     3301 attagagaaa gttcgattca atcgtgaaac tgaacaagat tttattctaa aagggacaat
     3361 tattgatgtt gagttttctg gagttacaat tcactataca ataaaagttt ctgaaagtca
     3421 gattcttaat gtaacaagta ttgatagtca ggctgctatt agatctgtcg gagaaagtgt
     3481 ggaattattt atcacaccat cagacgttct gcaattttaa ggaggtgcag tatgcgtcat
     3541 aaattaaatt taaaagattg gcttattcgt ttagggttaa tctggttctt agtaacattt
     3601 attatttatc caaactttga tctagtagtg aatgtatttg taaaaggagg agaattttcc
     3661 cttgatgctg tacatcgtgt tctaaaatct cagagggcac ttcagagtat tatgaacagt
     3721 tttaagttag cattttcact cattattaca gttaatgtcg taggtattct ttgtgttcta
     3781 tttacagagt actttgatat taaaggtgct aaaattttaa aattaggtta tatgacctct
     3841 ttaatttatg gaggagtggt tttagcgact ggatataaat ttgtctatgg tccttatgga
     3901 ttgattacaa aatttttaca aaatgttatc ccttctttag actctaactg gtttattggg
     3961 tatggtgcag tcttattcat tatgacattt tcaggaactg ctaatcatac attgttttta
     4021 acaaatacaa ttcgaagcgt tgactatcac actattgagg ctgctcgaaa tatgggagca
     4081 aaaccattta ctgttttccg aaaagtagtg ttaccaacct taattccaac tctatttgca
     4141 cttactatta tggtttttct tagtggttta tctgcagtag cagcacccat gattgttggt
     4201 ggtaaagaat ttcaaactat aaatccaatg attattacat ttgcagggat ggggaattct
     4261 cgtgatttag ctgccttact tgcaattatt ttaggtattg caactacaat tttgcttact
     4321 atcatgaata agatagaaaa aggtggaaat tatatttcta tctctaagac taaagcgcct
     4381 cttaaaaaac aaaaaattgc gtctaagcct tggaatatca ttgctcacat tgtagcatat
     4441 ggattgttca cagttttcat gcttccacta atttttatag tattatactc atttacagat
     4501 ccagttgcaa ttcaaacagg taacttaaca ttatcaaact ttactttaga aaattatcgc
     4561 ttattcttta gtaatagtgc ggcattctct ccattcttgg tcagctttat ttattctatt
     4621 attgctgcga caacagcaac aattctcgca gttgtatttg ctcgtgttgt cagaaaacat
     4681 aaatctcgtt ttgatttctt atttgaatat ggtgctctac ttccttggtt actaccaagt
     4741 acacttttag cagtaagttt attatttact tttaatcagc cacaatttct tgtcttgaat
     4801 cagattttgg taggtagttt ggtaattcta cttattgcat atatagttgt aaaaatccca
     4861 ttttcttata gaatggtacg tgctatttta tttagtgttg atgatgagat ggaagatgca
     4921 gcaagaagta tgggtgcttc acctttttat actatgatga aggttatcat tccatttatt
     4981 ttaccggttg ttctctctgt tattgcttta aactttaact ctttattaac tgacttcgac
     5041 ttatctgtat tcctttacca tcccctagct caaccattag gtattacgat tcgatctgca
     5101 ggtgatgaaa cagcaacatc taatgcacaa gctctggtat ttgtttatac aattgttctg
     5161 atgattattt ctggaacggt attatacttc acacaaagac cggggcgtaa agtaaggaaa
     5221 taatcatgac agccactagt cttgggttat caaatattga aatagttgtc aggattgttt
     5281 tatcagtagt cattggtagt ataattggtt tagagagagg gagcaaatcc cagcctgcag
     5341 gcatccgaac ttatagtatt gtttgtctag ctgcatgttt gattatgatg acgaatgaat
     5401 acgtatctta taaatttggg acaggagatc ctacacgatt aggagctcaa gttatatcag
     5461 gtgtgggttt tctaggcgct ggaacgattc ttattacaga taaaaagaaa attacaggtc
     5521 tgacaactgc agcaggcatt tgggcttcgg caggaattgg attagctatt ggagtaggtt
     5581 tttatgaggg agctctttta gtagccattt ctgtttgggg tgtgatatcc atgttccagc
     5641 cactaaaaaa atatctgcaa aatcgttcta aaatgattga attgtatata gtggttaaat
     5701 ctacagaagt ctataatcgt gtattggttt attgtgcaga aaatggtatc agaatgaccg
     5761 attcaagaac agcgtttgga gatgttaatt ctgacagaat tgaatacttt gatgttccgg
     5821 acaaaaaaat agcgtcattt attactctgg aattatcagg tagatttgaa catcttcgac
     5881 tgatggaaga aattgctaat attgtaggtg ttatttatgt cgaagaaatt agctgaaatt
     5941 tagtaaacaa agggacagat gtttttgtcc ttttgttttt tagatgtttt aatcatctaa
     6001 tttttgagta gatttttgct cagaggtttt tatgaaagat cataaaatca attttgattt
     6061 aggtaaaata gaatattttg ataataattg tttgatacaa gtatacaaat ttatttcttt
     6121 ttatgatatt tgtgaaatgg tgtttgcctt tcatttacct cctgatgagt tgataacaaa
     6181 tgttatcttt aaagaaaaaa ttaactctat
//
LOCUS       DAVKWS010000082         6094 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKWS010000082 DAVKWS010000000
VERSION     DAVKWS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.083"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(102..377)
                     /locus_tag="VW852_001931"
     CDS             complement(102..377)
                     /locus_tag="VW852_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV0273009.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(483..1322)
                     /locus_tag="VW852_001932"
     CDS             complement(483..1322)
                     /locus_tag="VW852_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0273010.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1464..2312
                     /locus_tag="VW852_001933"
     CDS             1464..2312
                     /locus_tag="VW852_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0273011.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2405..3322)
                     /locus_tag="VW852_001934"
     CDS             complement(2405..3322)
                     /locus_tag="VW852_001934"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV0273012.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWY"
     gene            3757..3963
                     /locus_tag="VW852_001935"
     CDS             3757..3963
                     /locus_tag="VW852_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1980 domain-containing protein"
                     /protein_id="HEV0273013.1"
                     /translation="MTAFQQLPSSVLQTGLFFSPSVSLDSQTVSAKEYLFPYQKERAQ
                     TTQTSEGTTSQYLKPDSSFYSQLK"
     gene            complement(4168..4461)
                     /gene="rpmA"
                     /locus_tag="VW852_001936"
     CDS             complement(4168..4461)
                     /gene="rpmA"
                     /locus_tag="VW852_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV0273014.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4478..4822)
                     /locus_tag="VW852_001937"
     CDS             complement(4478..4822)
                     /locus_tag="VW852_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV0273015.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4838..5152)
                     /gene="rplU"
                     /locus_tag="VW852_001938"
     CDS             complement(4838..5152)
                     /gene="rplU"
                     /locus_tag="VW852_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV0273016.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5188..5275)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5340..6038)
                     /locus_tag="VW852_001939"
     CDS             complement(5340..6038)
                     /locus_tag="VW852_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV0273017.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
BASE COUNT         1867 a         1265 c         1007 g         1955 t
ORIGIN      
        1 gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa gcctactgac
       61 caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac agcgtcttta
      121 agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat ttctttacca
      181 gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt accaaaaccg
      241 atcaattgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa tacagcttca
      301 actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt agcgatcaaa
      361 tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac aaatataatc
      421 atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat tttatttatt
      481 ttttaggagt ggtaacgtat caaaatagcc caagcgttct cacccgtgtg agtttgaata
      541 atggaacccg tttccaaaac agaaattggc ttttcaacat aagcttgtaa gctttctttc
      601 atctcttttg cccaatcatc actaccagaa tatgaaattc caatctctgc tacagcacgt
      661 tcagaaagcg atgttatcaa ctcatctaac cattttttaa atgttttagt tccacgacct
      721 ttaaccattg gctgcaattc atggtctttc atttgcatga caacacggat attgagaagg
      781 gagctcaaca agccagttac acggccaatt cgtccacctt tgacaagatt ttccaaagtt
      841 gaaacaccaa tataaagctc tgtatggttt ttaacctctt ctacatgaga taaaattgcc
      901 tccatatctt taccttcttg agctaacttc gcagcctcaa caacttggaa tttcagggct
      961 tggtcagtga aggaactatc aacaacagtc acatctgcag tagataggct agcaccttgg
     1021 cgtgctgctt ctaccgtacc cgaaagagca tgggacatat gaatagcaag aatctggcca
     1081 ccatctttgc ataggtcttc aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt
     1141 ttcggaagat tcttactttc ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc
     1201 gcatcagaat aaacaacatt atcaatcatt acagataatg gaacaattgt aatatctaat
     1261 tgctttacta gttcaggttc aatagtaaca gatgaatcgg ttacaatctt aatttttgtc
     1321 atagtatcaa tctttctatt ttaggattca gattggtttc cttacttcta attatatcaa
     1381 aaaaaagatt aaaaatccta atggagtcaa tcaaattttc cgtaaaattt gatataatca
     1441 acttataaga aaagaggtgt cctatgatta aaaaaattta ccccattttt accattttac
     1501 taggtgctgc tatttatgct tttggactga cttattttgt agttccccat catctctttg
     1561 aaggaggggc gacaggcatt accctcatca ccttttatct ttttaaaatc cctgtttccc
     1621 tcatgaacct gctgattaat attccccttt tcatcctagc ttggaagatc tttggagcca
     1681 aatccctcta ttctagttta ctaggaacct tagctttgtc cgcctggtta gctttttttg
     1741 agcatattcc ccttcatatt gatcttcaag gtgatttact aatcacagcc cttatagcgg
     1801 gaatcctatt gggaattggc cttggaatta tttttaatgc tggaggtaca actggcggaa
     1861 ctgatattct agctcgtatt ctcaacaaat acactcatat atccatagga aaactgctct
     1921 ttatcttaga tttttgtatt ctcatgttga ttctcctaat cttcaaggat ttgagattgg
     1981 tttcctacac gcttttgttt gattttattg tttctcgtgt tattgatttg attggtgaag
     2041 gaggatatgc cggcaaaggc tttatgatta tcacaaaacg tcctgaccaa cttgctaagg
     2101 cgattaatga tgacctcgga agaggtgtta cttttatttc tggtcaaggc tactatagta
     2161 aagaaaattt gaaaatcatc tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg
     2221 aaatgattca tcgaatcgat cctcaagcct ttataactat tacagaagcc catgaaatcc
     2281 taggagaagg cttcaccttt gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt
     2341 tagactaaat catctatctt ttgggttaca gacaacctct tttttatttt atttactcaa
     2401 gctcttaata ccaattccga gttacttctt catcagcctt taactgatcc actaattggt
     2461 caactgagtc aaatttggtc atatctcgaa tgcgatcaag ccaataaacc atgacggttt
     2521 ccccataaat atcttgatta aaatcaaaaa tattgacttc aaaacgtgct tcttctccat
     2581 caaaggtcac atttttcccg acactagcca tagcacgata cttctgtctt tgaatctcaa
     2641 catcaacaac ataaacgcca tctgctggca tataagtacg gtctaaaagc actaaattcg
     2701 ctgtcggata accaattgta cgaccacgag cattaccatg aaccaccata cctcttgatg
     2761 gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt tccatctaaa atagcttgac
     2821 ggatacgagt tgaactaatc tttcctttct catcttctac aggtggaaca atgataactt
     2881 ctccatcaaa gtaatccttt aaatcttctg ctgttttttt gtcagaacca aatgtataat
     2941 caaaacctgc aacaataatt ttggcattca tagccttgat ataagttgca aagaattctt
     3001 gtgcagtgag actagcgaat tgactactaa aatcaaggag atataattct tctacacctt
     3061 cgcgctttaa ttttctttca cgttcagcag ggttcaaaat atgcaaaaac aaatctggat
     3121 gataaggctc taaagcgatc tttggagatt cattaaaggt cataacgacg ataggcaaca
     3181 aatcctttct cgcagccttg ttggcaacac gaaataattc ttgatgcccc ttatgtatgc
     3241 catcaaaata gccgagaaca acgactgaat cagatggtgt gccaatatct ttttggtttt
     3301 ttataggaat agtaataatc ataaaataat tatatcatag cgatagctat ttctggaaca
     3361 gaaaatctga aatgttgttt ttttcacatg aagtgtacct gttttcaaaa agcactttat
     3421 tctatcgttg cttaactatg aactttgcaa tattcttctc aaaaacttgt aggacatctt
     3481 caaaattttg caaggagtga ttagacttgt tcggtaacca taaagtgtca tactatgctt
     3541 atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat tggtgccgaa
     3601 aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa tgagacaact
     3661 tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc actttccctg
     3721 atgacaaaaa aagccaaagc agtaaagaaa aactagatga ctgctttcca acaacttcca
     3781 tctagtgtgc ttcagactgg gctatttttc tctccatctg ttagcttgga ttctcagacc
     3841 gtttcagcta aagaatatct tttcccttat cagaaggaac gggctcaaac cactcagaca
     3901 agtgaaggga cgacaagcca atatttgaaa ccagatagca gtttttatag tcaattgaaa
     3961 taaaatctga agaaatcgag taggaaactc atatcaattt ttaacagtgt tctattccag
     4021 attcaatcca ctatacatat taaaaagtat gggtaccaaa acaaaaaaac aggctctccg
     4081 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4141 tcgggataaa aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt
     4201 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4261 gtctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4321 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg
     4381 tgaatcacgt ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4441 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4501 tttggacgaa ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4561 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4621 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4681 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4741 attcgccact ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc
     4801 tctcaaagac tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga
     4861 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     4921 acttgtaagt aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     4981 ctccagcaac aagtggagtt ccgacaacag tattttcacc accaacaaga acaacttcgt
     5041 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa
     5101 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5161 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5221 gaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5281 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5341 caagcttttt cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg
     5401 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5461 gaaaagacaa cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc
     5521 aaaaaaggag tgtgcttggt tcggaaataa tctccaaaag ccagcatggt ccgtttgata
     5581 ttccctgtca taaaagcgtt attataggca atacccgaca cttctccaaa agcagttgtc
     5641 accagtccca tacagaaggc caagggcggc actagataga tattatccac agtttgcggc
     5701 acaaaagcaa taatgattga taagattgcc aagggaatca aggacagaat aggttttttc
     5761 acaattctca atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctagc
     5821 aaggtgagaa ccttgtccct aacatccgaa acattatttt taattaattc tactgaaaga
     5881 aagacaacat ttccagtttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa
     5941 gcatccacat atccagcaca aaacgtcaaa aaaagtgcta accttttaga ctgacgtgat
     6001 atttttctta taggtaataa cctcattttc cctcccattg tattttctct tagaaatatt
     6061 gtaccatttc tttctaaaaa atcgtagact acca
//
LOCUS       DAVKWS010000083         5901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKWS010000083 DAVKWS010000000
VERSION     DAVKWS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.084"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            203..1903
                     /locus_tag="VW852_001940"
     CDS             203..1903
                     /locus_tag="VW852_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV0273018.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1896..2372
                     /gene="ilvN"
                     /locus_tag="VW852_001941"
     CDS             1896..2372
                     /gene="ilvN"
                     /locus_tag="VW852_001941"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV0273019.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2438..3460
                     /gene="ilvC"
                     /locus_tag="VW852_001942"
     CDS             2438..3460
                     /gene="ilvC"
                     /locus_tag="VW852_001942"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0273020.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPSLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3567..3803
                     /locus_tag="VW852_001943"
     CDS             3567..3803
                     /locus_tag="VW852_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273021.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            4041..4397
                     /locus_tag="VW852_001944"
     CDS             4041..4397
                     /locus_tag="VW852_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0273022.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4447..5697
                     /gene="ilvA"
                     /locus_tag="VW852_001945"
     CDS             4447..5697
                     /gene="ilvA"
                     /locus_tag="VW852_001945"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV0273023.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1827 a          987 c         1353 g         1734 t
ORIGIN      
        1 ccgattaatg aaggactgag aaatcagttc ttttttcttt tttagtaaat gaaatcggta
       61 tctttttaat aaaaacaaaa ataacattca taaataaacg ttaaaataga aaattcagaa
      121 aatttcttct tttatcttga aaaattttga aaaaatggta tgatagtaac aagttatttt
      181 taagaggaaa gaaaggggaa caatggagaa aatcagttta gaatctccta agacggggtc
      241 ggacctagtt ttggaaacac ttcgtgattt aggagttgat accatctttg gttatcctgg
      301 tggtgcggtt ttgccttttt atgatgcgat atataatttt aaaggcattc gccacattct
      361 agggcgccat gagcaaggtt gtttgcatga agctgaaggt tatgccaaat caactggaaa
      421 gttgggtgtt gccgtcgtca ctagtggacc aggagcaaca aatgccatta cagggattgc
      481 ggatgccatg agcgatagcg ttcccctttt ggtctttaca ggtcaggtgg cgcgagcagg
      541 gattgggaag gatgcctttc aggaggcaga catcgtggga attaccatgc caatcactaa
      601 gtacaattac caagttcgtg agacagctga tattccgcgt atcattacgg aagctgtcca
      661 tatcgcaact acaggccgtc cagggccagt tgtaattgac ctaccaaaag acatatctgc
      721 tttagaaaca gacttcattt attcaccaga agtgaattta ccaagttatc agccgactct
      781 tgagccgaat gatatgcaaa tcaagaaaat cttgaagcaa ttgtccaagg ctaaaaagcc
      841 agtcttgtta gctggtggtg gaattagtta tgctgaggct gctacggaac taaatgaatt
      901 tgcagaacgc tatcaaattc cagtggtaac cagtcttttg ggacaaggaa cgattgcaac
      961 gagtcaccca ctctttcttg gaatgggagg catgcacggg tcattcgcag caaatattgc
     1021 catgacggaa gcggacttta tgattagtat tggttctcgt ttcgatgacc gtttgacggg
     1081 gaatcctaag actttcgcta agaatgctaa ggttgcccac attgatattg acccagctga
     1141 gattggcaag attatcagtg cagacattcc tgtagttgga gatgctaaga aggccttgca
     1201 aatgttgcta gcagaaccaa cagttcacaa caatactgaa aagtggattg agaaagtcac
     1261 taaagacaag aatcgtgttc gttcttatga caagaaagag cgtgtggttc aaccgcaagc
     1321 agttattgaa cgaattggtg aattgacgaa tggagatgcc attgtggtaa cagacgttgg
     1381 tcaacaccaa atgtggacag ctcagtatta tccctaccaa aatgaacgtc agttagtgac
     1441 ttcaggtggt ttgggaacaa tgggctttgg aattccagca gcaatcggtg ctaaaattgc
     1501 taacccagat aaggaagtag tcttgtttgt tggggatggt ggtttccaaa tgaccaacca
     1561 ggagttggct attttgaata tttacaaggt gccaatcaag gtggttatgc tgaacaatca
     1621 ttcactcgga atggttcgcc agtggcagga atccttctat gaaggcagaa catcagagtc
     1681 ggtctttgat acccttcctg atttccaatt gatggcgcag gcttatggta ttaaaaacta
     1741 taagtttgac aatcctgaga ccttggctca agaccttgaa gtcatcactg aggatgttcc
     1801 tatgctaatt gaggtagata tttctcgtaa ggaacaggtg ttaccaatgg taccggctgg
     1861 taagagtaat catgagatgt tgggggtgca gttccatgcg tagaatgtta acagcaaaac
     1921 tacaaaatcg ttcaggagtc ctcaatcgct ttacaggtgt cctatctcgt cgtcaggtta
     1981 atattgaaag catctctgtt ggagcaacag aagatccgaa tgtatcgcgt atcactatta
     2041 ttattgatgt tgcttctcat gatgaagtgg agcaaatcat caaacagctc aatcgtcaga
     2101 ttgatgtgat tcgcattcga gatattacag acaagcctca tttggagcgc gaggtgattt
     2161 tggttaagat gtcagcgcca gctgagaaga gagctgagat tttagcgatt attcaacctt
     2221 tccgtgcaac agtggtagac gtagcgccga gctcgattac cattcagatg acgggaaatg
     2281 cagaaaagag cgaagcccta ttgcgagtca ttcgcccata cggtattcgc aatattgctc
     2341 gaacgggtgc aactggattt acccgcgatt aaaaatccaa cttaaattta ttaaaccagc
     2401 ctaaaaggca ataaataata gaaaagagag aaaagctatg acagttcaaa tggaatatga
     2461 aaaagatgtt aaagtagcag cacttgacgg taaaaaaatc gccgttatcg gttatggttc
     2521 acaagggcat gcgcatgctc aaaacttgcg tgattcaggt cgtgacgtta ttatcggtgt
     2581 acgtccaggt aaatcttttg ataaagcaaa agaagatgga tttgatactt acacagtagc
     2641 agaagctact aagttggctg atgttatcat gatcttggcg ccagacgaaa ttcaacaaga
     2701 attgtacgaa gcagaaatcg ctccaagctt ggaagctgga aacgcagttg gatttgccca
     2761 tggtttcaac atccactttg aatttatcaa agttcctgcg gatgtagatg tcttcatgtg
     2821 tgctcctaaa ggaccaggac acttggtacg tcgtacttac gaagaaggat ttggtgttcc
     2881 agctctttat gcagtatacc aagatgcaac aggaaatgct aaaaacattg ctatggactg
     2941 gtgtaaaggt gttggagcgg ctcgtgtagg tcttcttgaa acaacttaca aagaagaaac
     3001 tgaagaagat ttgtttggtg aacaagctgt actttgtggt ggtttgactg cccttatcga
     3061 agcaggtttc gaagtcttga cagaagcagg ttacgctcca gaattggctt actttgaagt
     3121 tcttcacgaa atgaaattga tcgttgactt gatctacgaa ggtggattca agaaaatgcg
     3181 tcaatctatt tcaaacactg ctgaatacgg tgactatgta tcaggtccac gtgtaatcac
     3241 tgaacaagtt aaagaaaata tgaaggctgt cttggcagac atccaaaatg gtaaatttgc
     3301 aaatgacttt gtaaatgact ataaagctgg acgtccaaaa ttgactgctt accgtgaaca
     3361 agcagctaac cttgaaattg aaaaagttgg tgcagaattg cgtaaagcaa tgccatttgt
     3421 tggtaaaaac gacgatgatg cattcaaaat ctataactaa ttagaaatat atagcgctgg
     3481 agatgatttt atgaaaaaga ttatgagaaa aattgcatcg ttattattgg ttctagttgt
     3541 ataatgtaat tacaccgtcg gtaatagtgc tagcagacca aaataaagca gattggtcgt
     3601 atgatgaaaa tgctgtaatt aacatttatg atgatgctaa ttttgaagat ggtaggttgc
     3661 atatgacctt tgaacaattc ttcaaattgg cacaaatagc tagagaagaa ggtcttgaaa
     3721 ttcattctcc gtttgagaga gctggtgcga ctaaatctgc tcgttatata gcgaaatgga
     3781 ttttgagaaa taaaaaacat taacaaatat agttggtaaa tcattaggac ctaaatcagc
     3841 tgttagattc ggagaagctt tatcctatat tgaaggtcct cttcgcagaa taaatgagac
     3901 gatagatggc ggtttatatc aaatagagca aattattgca tctggattga aagaatcggg
     3961 tttaaatgac tggactgcga aaactttagc ttcagctatt cgtgggatat tagatgtact
     4021 tatttagggg ttgaaatcat atgaatatta ccaatttgtt ttctatcaag acaggatgtg
     4081 atgaaactga taggcaactg caaaaactat tttttcagtt ggatttacaa ttgggagaat
     4141 tgacagatca actaagaaaa ttagattcta attttgttcc tcgtagtcaa tttgtagaca
     4201 cgttggattt gaatgatgta gaatataaag aaattttaaa ctattttatc ttccatcgta
     4261 atgatagtga agaaagtttg gtagaatggt tatatgattg gatttccaca aatcgttatg
     4321 aacttcctaa agagttttcg attcgtatgg ctcataaata ccatgaaagt gttactgaag
     4381 ttttcggaga tgaataacta aaaaacagtc attagtgact gttttttata gaaaaagagg
     4441 ttttatatgt taagttcaaa agatataatc aaggctcaca aggtcttgaa cggtgtggtt
     4501 gtgaatactc cactggatta cgatcattat ttatcggaga agtatggtgc taagatttat
     4561 ttgaaaaaag aaaatgccca gcgtgttcgc tcctttaaaa ttcgtggtgc ctattatgcc
     4621 atttcccagc tcagcaagga agaacgtgaa cgtggggtag tctgcgcttc tgcgggaaat
     4681 catgcgcagg gagtagccta tacttgtaat gaaatgaaaa ttcctgctac tatctttatg
     4741 cctattacta cgccacaaca aaagattggt caggttcgct tttttggtgg ggattttgta
     4801 actattaaac tagttggaga tacctttgat gcctcagcca aagcagctca agaatttaca
     4861 gtctctgaaa atcgtacctt tattgatcct tttgatgatg ctcatgttca agcaggtcaa
     4921 ggaacagttg cttatgagat tttagaagaa gctcgaaaag aatcgattga ttttgatgct
     4981 gtcttggttc ctgttggtgg tggcggtctc attgccgggg tttctaccta tatcaaggaa
     5041 acaagtccag agattgaggt tatcggagta gaggcgaatg gagcgcgttc catgaaagct
     5101 gcctttgagg ctggaggtcc agtcaaactc aaggaaattg ataaatttgc ggatgggatt
     5161 gctgtgcaaa aggtaggtca gttgacctat gaagcaactc gtcaacatat tcaaactttg
     5221 gtaggtgtcg atgagggatt gatttctgaa accttgattg acctttactc taagcaaggg
     5281 atagtcgcag aacctgctgg agcggctagt atcgcctctt tagaggtttt agctgaatat
     5341 attaagggga aaaccatttg ttgtatcatt tctggaggaa ataatgatat caaccgtatg
     5401 ccagaaatgg aagagcgtgc cttgatttat gatggtatca aacattactt tgtggtcaat
     5461 ttcccacagc gtccaggagc tttgcgtgag tttgtaaatg atatcctggg gccaaatgat
     5521 gatatcacac gttttgagta tatcaaacga gctagcaagg gaacaggccc agtattgatt
     5581 gggatcgctt tggcagataa gcatgattat gcaggtttga tccgtagaat ggaaggtttt
     5641 gatccagctt atattaactt aaatggtaat gaaacgcttt ataatatgct tgtctgagga
     5701 ctaataaaaa aatatcatat tatttgtaca actgatgtat atgtgctata atgaagatga
     5761 agaaagggga cgattcctat ggttttagaa aacttatcgt gcaattctta cttctgttca
     5821 ttatagcact tatgatgttt ggctcagatt tccatccttc attttgattt cctatctatt
     5881 gacaagcata gtcatactgt c
//
LOCUS       DAVKWS010000084         5883 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKWS010000084 DAVKWS010000000
VERSION     DAVKWS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.085"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..116
                     /locus_tag="VW852_001946"
     CDS             <1..116
                     /locus_tag="VW852_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV0273024.1"
                     /translation="TLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
     gene            complement(271..594)
                     /gene="tnpB"
                     /locus_tag="VW852_001947"
     CDS             complement(271..594)
                     /gene="tnpB"
                     /locus_tag="VW852_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0273025.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(575..760)
                     /locus_tag="VW852_001948"
     CDS             complement(575..760)
                     /locus_tag="VW852_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273026.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(1033..1644)
                     /locus_tag="VW852_001949"
     CDS             complement(1033..1644)
                     /locus_tag="VW852_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV0273027.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            complement(1667..3052)
                     /locus_tag="VW852_001950"
     CDS             complement(1667..3052)
                     /locus_tag="VW852_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV0273028.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            complement(3053..4828)
                     /locus_tag="VW852_001951"
     CDS             complement(3053..4828)
                     /locus_tag="VW852_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV0273029.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            complement(4891..5211)
                     /locus_tag="VW852_001952"
     CDS             complement(4891..5211)
                     /locus_tag="VW852_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV0273030.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            complement(5201..>5883)
                     /locus_tag="VW852_001953"
     CDS             complement(5201..>5883)
                     /locus_tag="VW852_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV0273031.1"
                     /translation="DFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVREVESIWNEY
                     ETFNNIRVLDVGADLAYFKHLKLLSNELDEVLSQVIVEMIDFYNIITVKRGLSQNKSH
                     GDILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSE
                     LEFLCDLLLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKE
                     RIRPHYGS"
BASE COUNT         1903 a         1206 c          875 g         1899 t
ORIGIN      
        1 aaactttgga agatgtcatg aatcaactcc aagatgttat acaaggactg gagaaggagg
       61 tgataaagtc catcgttaat cggagatgga ctagaatgct ttttgaaagc agatgagtat
      121 aaaaagaaag tcctcatttc aatagaaatc acgactttct gatggattta tagtaaaatg
      181 aaataagaac aggacaaatc gatcaggaca gtaaaatcga tttctaacaa tgttttagaa
      241 gcagaggtgt actattctag tttcaatcta ctatattttt ggagtgatag aaaagccctt
      301 cataagccag tctacttgtt ctggtgtgag agctttgaca tccttttctg tacttagcca
      361 aatcaatctg ccgttctcaa agcgtttata tagtagccaa aatccttgac catcccagta
      421 aaggacttta aagcggtctt tacgtccacc acaaaagaga aagacttgac cggagaaagg
      481 atccaattca aagtgggttt taaccagata agccagtgaa tcgattcctt gtctcatatc
      541 agttttccca cacacgagat agacctcccc taggctagat agatgaattg tcatagagca
      601 acaccttatc caaaagctgt tctaccattt cgagattgag agattgaaaa aaacttactt
      661 caagcttgcc aatacgaatt ttaagcagaa tatcatttct ctttttagaa tcaaaacgac
      721 gagattgtgg aatctctaca ggaacaatag gttgtggcat taaaatatcc tccaatagtt
      781 ttactttact aatagtatac aggaggagct ccatgaatga tagatacctt gttatgacgc
      841 gcttactttc tatataatta catttacgat ttactaatcc taaatctaga ttagtagaaa
      901 cattttcatt atctaacaaa ccaaaatttt gaaaaagata tcctgatact gtcaataaaa
      961 aaagcttaga aaggcttaaa gccttatcca agctgatatt tgttcatcta attaataatc
     1021 taaataaagg atctacttca ctttcataat acgaactaaa ttggctcttt ctgcctcctc
     1081 tagtttcaat tctatataat gaatagtttc cgacaagttt ggaataatcg agtattctaa
     1141 accatttaca cgtctacgtg ttttttctat ttcatcagcc attaactgac acgttttttc
     1201 aacttctgcc agtcttagta atttataaat taaactattc attgtagcaa atacatcatc
     1261 catttcacta ttagaagata aatagctgta ttcactgttc tcattttgag aagtaatatt
     1321 catatgcatt ctaggaactg ttacactcat gatattttct ttctcaacaa ataattcaat
     1381 ttctttcgat ggaattgaaa ataattcctc caccatttga gaattcttta atgatttagc
     1441 aactgcaaag gattttagat tatcaattag ataactttcc acttctttcc gaagttgatt
     1501 attctcacga atcaaagaaa taaatcgcct catcaattca tctcttttat cctttaataa
     1561 cttatgtcca cgttcagctg ttgtcaaacg ttcctttaag ttattcaatt ccatacgagt
     1621 tggttttaca ttcaaacgta ccatagataa tcactccatt tccggattaa acttctacca
     1681 aaggtaagta tttatcaagc aaatcatctt tgatacgttt taactctgtt ctaggaagaa
     1741 ttgatagtaa ttcccaccca agattcaacg tatcttctat atttcgattt ttataaaatc
     1801 cttggtttat gtactcttct tcaaaacgct ttgtaaacct cacatacaat ttatctacat
     1861 cagataaagc cgattctcct aatactactg ctaactcttc aacctttttc ccttgggcat
     1921 aggctgcaaa cagttgattc atagttggag catgatctcc acgagtttta ccttctccag
     1981 atcccttatc ttttaatcga gagagagaag gtaaaacatt gattggtgga cgataacctt
     2041 gattatacaa ctcatgcgac aaaataattt gcccttcagt aatgtatcca gttaaatcag
     2101 gaattggatg tgttatgtca tcttctggca ttgttaaaat aggaatctgt gtcaccgaac
     2161 cttttttacc aactaagcga ccagcccttt cgtatagagt tgataaattt gtatataaat
     2221 atcccggata gcctcgtctc cctggaactt cacggcgagc tgccgagact tcacgtaacg
     2281 cttcacaata gttagtcata tccgtcatga taactagaac gtgcatatct ttttcaaaag
     2341 ctagatactc tgccgcagtt aaagcaatgc ggggagttgc aatacgctca attgcaggat
     2401 catttgccaa gttcataaat aaaaccgaac gatcgatcgc tcctgttttt ctgagttctt
     2461 ccataaaaaa ctcagcttct tcaaaagtaa tacccattgc tgcaaataca accgcaaaat
     2521 tttcatcaga atttaaaaca gtcgcttgtc ttgctatctg agcagctaat tcattatgag
     2581 gtaagcccga acctgaaaat actggtaatt tttgaccacg tacaagagta ttcaaatgat
     2641 caatagagga gatccctgtc tgaataaatt catctggata atctctagat acaggattaa
     2701 tagcttgacc atcaatatct aaatatttct ctggaattaa atctggtcca ccatcaattg
     2761 gttttcccat cccattaaaa atacgaccaa ccatatcctc agatacagcc aattctaatg
     2821 catgaccagc aaaacgaatt ttagactttt ctaaatttat tccactagat ccttcaaaaa
     2881 gctgaaccat tgctttatct tcgtggatct ctaaaacttg tccacgacga atttctccat
     2941 tatgaagttg aatttcaact aactcattgt aagacacatt atttacttgt tcaacaatca
     3001 taagaggccc aacaacttca ctagcagttc tgtattcttt tataacactc atttatagac
     3061 ctcctgtttc taatatcaaa tgaatttgat gtgtaatctc atttgatata attttgattt
     3121 catctaatct atcttctgaa acatatttac ttctcgccat acggtctcga actgccacgg
     3181 taccttccat aatctctgta aagtaagaac ccaactctaa agcatgattt gcctgatcag
     3241 caaaagtgag aatattactt agcattgctt cttgttttgc aaacgaagtg aatgtatcta
     3301 ccgaatcaaa agcgttctgt tgcaaataat cttctcgaat ttgtttagca atttccatcg
     3361 ttagtcgttc attatcagac agagaatcaa ttccaacaag acgaacaatt tcctctaaac
     3421 tagattcccg ttgtaagtag ttcatcgcac gagttatttt actattccaa tctgtcttct
     3481 ctttaccatc tatataagtg cccacactgt ctttatatag tgaataagat gtaagccagt
     3541 taattgcagg aaaatgacgt cgctgtgcca acggagcatc aagcccccaa aaaactttca
     3601 caatccgtaa agtgttttga gtaactggtt ctgaaatatc tccaccaggt ggcgatacag
     3661 ctccaatagc agtaatcgtt ccttcacgtt ctggaagccc tagaacctga gaacgtcctg
     3721 ctctttcata atattcagcg atacgacttc ccagataagc aggataaccc tcatcaccag
     3781 gcatttcttc tagacgtcct gacatttcac gtagcgcttc tgcccaacgt gaagttgaat
     3841 cagccataat ggcgacagag tagcccatat cacgaaaata ctcagccatg gtaattcctg
     3901 tataaattga agcctcacga gcagcaacag gcatatttga agtattagca atcagaactg
     3961 tccgttgcat aattgattgt ccggtattag ggtcaatcaa ctcaggaaac tcattcagta
     4021 catccgtcat ttcatttcca cgttctccac aaccgacata aataacaata tcaacattgg
     4081 caaatttagc tacttggtgt tgtacaactg tctttcctgc tccaaacggt ccaggaactg
     4141 ctgcagctcc ccctttggtt actggaaaga atgcatcaat aactcgttga cctgtgatta
     4201 atggttcttc tggaattaaa cgtttagaaa caggacgcgc cttgcggaca ggccattttt
     4261 gcataagcgt tcctttatag aaactaccgt ccaatttttt tatttcatat acaacttcat
     4321 caattgtaaa atcgccagat gcaatagaaa cgacttctcc agatactcca taaggaacca
     4381 taattttatg attaactacc tcggtttcct tgacagttcc aagaatatca cccgtactca
     4441 ctttttgacc aattgctata gtggaatcaa aatgccactt aatatctcta tccaaacttg
     4501 gaacttctac cccacgaact agaaaatcat tatgagtagc caatttaaat cgatctaatg
     4561 ggcgttgtat gccatcaaac atttgagaaa tcaatcctgg ccctaattca accgagagag
     4621 gttctccagt tgtaacaaca ggttctcccg gaccaagacc agatgtttct tcatagactt
     4681 ggatagatgc ctgatctctt ctcatttcaa taatttcacc gattaaccct agcttaccta
     4741 cacggcaaat atcttgaata ttagcctcct gcatacctga tgcaataact agaggtcccg
     4801 atacttttat aatcttccct tgagtcaaag ttattctcct tactaagtcc aaaaattata
     4861 gaataatatt acagacaatt ttgtacatta ttataaaata ttgtgtccta ctgctttctc
     4921 tacattatcc tctatacgtt ttaatcctaa atttaaacct tgtttatgag tcggtaataa
     4981 aataatagca ggcacaactt gggaatcata atggcgaatt gtatctaata tcattgaagc
     5041 aatgtcttca gtgatataaa tgacaccata atcagattga gctaattttc ttagtgtatt
     5101 tatagcttct tgttcttggt aggcaggaaa tatatcaaac ccaatcatgc taaatggtaa
     5161 aataatatca cggctaccaa ttatgccaat tttatactta ttagcttcca taatgtgggc
     5221 gtatcctttc ttttattgat tcaaagggaa ttgtattttg aagagctgat atgatcattc
     5281 tgagattctt tacttcaaac tcacatccca ataaatatct agcaagaact aacggcccct
     5341 ctacattgta ccttccttga tctaaagttt tatacaatag taaatcacat aaaaattcta
     5401 actcagaaga tgaaattgtt gcgtcctgca tcttcaattc ataagttgaa aagattgaat
     5461 ctaagcttgg atttatttta ttgaaccaca caaatatttc ttgattttct acaatgtata
     5521 taaattcttt agcagaaata cttccttcat ctgaaagtaa ttgtaaaata tccccatgac
     5581 tcttattttg agataaacca cgttttacag taataatatt ataaaagtca atcatttcga
     5641 caataacctg agacagtacc tcatctaact cattagataa aagtttcaga tgtttaaaat
     5701 atgctagatc agctccgaca tcaagtacac gaatattatt aaaagtttca tactcattcc
     5761 aaattgattc tacttcacga accataaaat caggaagtgt atctgaatgt aaggaagaaa
     5821 ctaaatgttt taaactttct atatcaaaaa tccctattgg aattaataat ttagaaaaat
     5881 ctt
//
LOCUS       DAVKWS010000085         5484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKWS010000085 DAVKWS010000000
VERSION     DAVKWS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.086"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(46..1935)
                     /locus_tag="VW852_001954"
     CDS             complement(46..1935)
                     /locus_tag="VW852_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV0273032.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGIG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2073..3638
                     /locus_tag="VW852_001955"
     CDS             2073..3638
                     /locus_tag="VW852_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0273033.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3638..4402
                     /locus_tag="VW852_001956"
     CDS             3638..4402
                     /locus_tag="VW852_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0273034.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4601..4816)
                     /locus_tag="VW852_001957"
     CDS             complement(4601..4816)
                     /locus_tag="VW852_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273035.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
BASE COUNT         1721 a         1216 c          973 g         1574 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggctccgat
       61 actgccgcca cctccgcctc cagagaagcc accgccagaa cttccacttc cagaagatac
      121 ggagtaggta cttgctgtat ttgcgacact agcataatgg ctcatttgcg cgcttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagagattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat
      361 ctcacgtaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccta tacccacttg
      601 caaagtcttt tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac
      661 tcgttttctc actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg
      721 aagccctctt gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc
      781 agataccttg taatccgcaa acaaattgga aagagtggct tcttttttgc ctgaaaaggc
      841 tagatttaga cagtctctct caaatcttga caaaccatct tctttcacca atctcaagcc
      901 aactgcatct ccttctgaaa taatagagac attcccacgg tctatcacat ctagcaaggt
      961 agcttgaata agttgatcaa aggtgaattt tccagctccc ttgaccaagg gactcacttc
     1021 ctccaaggag gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggtgg
     1081 ttcatagaga cgatgatttt tggcatattt gactgaagga gtggtctttc ttctataaat
     1141 aaaatagaag cagacactca ataacaagga gatagaaagt atcgaaggga ggacccaagt
     1201 aacgagttgt ttactctgat ctttttctct aacaatcgag tcttctatct tattaaactc
     1261 ttctaaacga ttccctttca agccctgatc cctagcgcta gcaaaatcgg tccgagacca
     1321 ataggcatgc aactcaactc cacgcttagc cggaagattg tctaaacgga tagtataatc
     1381 aaggttactc ttttcaatcg ttccctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ttcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcagcc tgaacctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt ggcccttaaa gtcctcctca aactggtaaa ctatcttctg
     1801 tctaaactct gccgtattat ctgcatgaat atacaaatcc ccttgataag agtttatctt
     1861 gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac aagcctaaca ccagtaagaa
     1921 aaaagttttt ttcatcaact aagccccctt tttatctttc ttatcattat agcatctttt
     1981 cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagatcaga ataggagaaa tcatgagaaa aatatactta tctattttca
     2101 caagtctctt gctgatgcta ggacttgtca atgttgctca agccgatgaa tatttacgca
     2161 tcgggatgga agcagcatat gctcccttta actggaccca ggatgatgat agcaacggag
     2221 ctgtcaaaat cgatgggacc aatcagtatg ccaacggata cgatgttcaa atcgccaaga
     2281 aaatcgctaa ggacttaggt aaagaacctt tggttgttaa aaccaagtgg gaaggtctag
     2341 tccctgccct tacttctggt aagattgaca tgattatcgc aggtatgagt ccaactgcag
     2401 aacgcaaaca agaaattgcc ttttcgagca gttactacac tagcgaacca gttttgcttg
     2461 tcaaaaaaga ttctgcctac gcaagtgcta aatctttgga tgactttaac ggtgcaaaaa
     2521 tcacttctca acaaggggtc tacctctacg acttgattgc acaaatccca ggtgctaaaa
     2581 aagaaacagc catgggagac ttcgctcaaa tgcgacaagc tcttgaggct ggtgtcattg
     2641 atgcttatgt ttctgaacgt ccagaagcac tgactgctga agctgcgaac tctaagttca
     2701 agatgattca agtagaacct ggtttcaaaa ctggggaaga agatacagct atcgctatcg
     2761 ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc cagcattgaa accatttcaa
     2821 aagatgacca agttgccttg atggatcgta tgatcaagga acaacctgcc gaagctacaa
     2881 caactgaaga gactagcagt agtttcttta gccaagtcgc taaaattctt tctgaaaact
     2941 ggcaacaact cttgcgtggt gctggtatca ctcttttaat ctctatcgtc ggaaccatca
     3001 taggtctcat tattggactt gccattggtg tcttccgtac tgctcctctc tctgaaaaca
     3061 aagccattta tggccttcaa aaactagtcg gctgggttct caatgtctac attgaaattt
     3121 tccgtggtac gccaatgatt gttcaatcga tggttatcta ctatggaact gcccaagctt
     3181 tcggtatcaa ccttgaccgt acactagctg ctatcttcat cgtttcaatc aataccggtg
     3241 cctacatgac tgaaattgtc cgtggtggta tcctggcagt tgacaaggga caatttgaag
     3301 ctgcgactgc tcttggtatg actcataacc aaaccatgtg taagattgtc ctacctcagg
     3361 ttgtccgcaa tatcctacca gcaactggta atgaatttgt catcaatatc aaagatacat
     3421 ctgtattgaa cgttatctct gttgtcgaac tttatttctc aggaaatacc gtggcaacac
     3481 aaacctatca atacttccag acatttacaa tcatcgccgt gatttacttt gtcctcacct
     3541 tcaccgtaac acgtatccta cgctttatcg agcgcagaat ggacatggat acctacacta
     3601 caggtgctaa ccaaatgcaa acggaggatt tgaaataatg acacaagcaa tccttgaaat
     3661 taaacacctc aaaaaatcct atggacaaaa cgaagtgcta aaagacattt ctctcactgt
     3721 ccacaaggga gaagttattt ctatcatcgg aagctctgga agtgggaaat caaccttcct
     3781 acgctccatt aacctacttg aaacgccaac tgatggacaa atcctttatc atggacaaaa
     3841 cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa aagttaggga tggttttcca
     3901 atcctttaac ctctttgaaa atctcaacgt tcttgaaaac acaatcgtcg ctcagacaac
     3961 tgtccttaaa cgcgaacgca cagaagctga agaaattgcc aaagaaaacc tggaaaaggt
     4021 cggcatggga gaacgctact ggcaagcgag acccaaacaa ctctcaggtg gtcaaaaaca
     4081 acgtgtggcc atcgctcgtg ccctttcaat gaatcctgac gccattctct ttgatgaacc
     4141 aacatcagct ctcgatccag aaatggttgg agaggtcctc aaaatcatgc aagatctggc
     4201 tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc
     4261 tcaccgtgtt atctttatgg ataagggtgt gattgctgaa gaaggtaaac cagaagacct
     4321 cttcactaat cctaaagaag accgtacaaa agaatttctt caacgttatc tcatactcaa
     4381 tgaaaatcaa agagcaaact aggcagctag ccgcaggttg ctcaaaacac tgttttgagg
     4441 ttgcagatag agctgacgtg gtttgaagag attttcgaag agtatcaaat aaaaagaaaa
     4501 ggctgcatca accgtgcagt ctttttgctg tcctcaaaat gaaaaggcag attctctatc
     4561 tctactactt gaaggtcaag aatccaccgt tatccaaaga ttaatcttca aatttttcat
     4621 gatttttttc atagaaatca attaagccta gggctgtttc aaatgaaata ttttttactt
     4681 ttgcacgacc ctgcgccaga gcaatgatag acatttcacg cgcattcgtt tctttagaga
     4741 tacggtagcc tgtgattttc ttatcacgaa cccagccaac aactgattct actttttcaa
     4801 agtttgactt agccatgttt ttctcctatt ttcttcttta taatatttaa ttgtatacgt
     4861 ttttactact tttgtcaata aaaaaaacat atattcaacg aaaataatta gttcagaatt
     4921 ttcttacttc ctttttaaat ccgataatat ataggttttg ctagcctcta caccataaat
     4981 atttgctaat aaaaatactg tattattacc ctcttaaggt gcaaaattat tcaagttctt
     5041 aaaaaatgct tctctatttc cgtaagatag cttatttctt cgatttaaga actgaagatg
     5101 atagttctaa cctttagaaa tccaagcctt ctctagctca aaaactccta tctttcttcc
     5161 tcaataaatc atctgagaga taggtattcc ttatatcctt caaattcatc actcctttgg
     5221 atattgaggt gctagcctag aagcagacac ccgattatct aaaccatcct taatagagat
     5281 gacaagacag ataagctcag ttaattactt atatatcttt ttaatatggt atatttgtca
     5341 acaaaaagaa atcaaatcat accttatact caatgaaaat caaagagcaa actaggaagc
     5401 tagccgcaaa ttgctcaaaa cactgttttg aggtttcaga tagagctaac gtggtttgaa
     5461 gagattttcg aagagtatta aaga
//
LOCUS       DAVKWS010000086         5295 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKWS010000086 DAVKWS010000000
VERSION     DAVKWS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.087"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(277..796)
                     /locus_tag="VW852_001958"
                     /pseudo
     CDS             complement(277..796)
                     /locus_tag="VW852_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1146..1970)
                     /gene="nadE"
                     /locus_tag="VW852_001959"
     CDS             complement(1146..1970)
                     /gene="nadE"
                     /locus_tag="VW852_001959"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV0273036.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGFHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1967..3427)
                     /locus_tag="VW852_001960"
     CDS             complement(1967..3427)
                     /locus_tag="VW852_001960"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV0273037.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEGLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3543..4031)
                     /locus_tag="VW852_001961"
     CDS             complement(3543..4031)
                     /locus_tag="VW852_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273038.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESLYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4021..4230)
                     /locus_tag="VW852_001962"
     CDS             complement(4021..4230)
                     /locus_tag="VW852_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0273039.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(4615..5067)
                     /locus_tag="VW852_001963"
     CDS             complement(4615..5067)
                     /locus_tag="VW852_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273040.1"
                     /translation="MIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPLFVASNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
BASE COUNT         1603 a         1162 c          965 g         1565 t
ORIGIN      
        1 tcgatagagg attctatttt aaatttaaca acagctagga ttacactata aaaagtacat
       61 taccacctgt tcggctggag aatctagtcg attagctccc tcttagttac aagcttaaag
      121 cgatttttta actaatacta aaccagatta aagaaggaac tagaacatct gtcttgtact
      181 aatcaatatg ctattttaaa gtttcaaata aacttggaat gctaattatt atatttacaa
      241 taaatagttg acttcgatag aaacagaaaa tagaattcaa cattcttccc tacttatacc
      301 ataataaata agatccgcaa gatagccttt tctctctaca ccattaacaa tggtttgtag
      361 ataggacatt ccagcctttt ccatgacacg acctgaagct gggttgagac tggcataacg
      421 tgctctgacc ttttgaaaac ctgcttgagt aaaacaaaag tccaagacag ctttcaaggc
      481 ctctgtcatc ataccatgtc cccagtaagc cttgcctagc acatagccga tttcacagct
      541 taaatcagct cacttgctct gggttttctt ttagacaaat ggcccatttg taatagttga
      601 gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg acatcaggat
      661 ggggatccca ggtaacatag gtcagattct cagcggatga agcccaattt tgaaacatgg
      721 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
      781 taccgataga tttcatgatg ctacctcact tccttatttc tatgctcgta gtaaaaaggc
      841 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agtcgctgcc
      901 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
      961 gataaaatcg aactcttcga gttaccgaat ctgcctcatt ttcttatttc taaactcggg
     1021 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
     1081 ccactggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtcccccgga
     1141 cctttttact cccaaaagtc atcaaataca gtgatgggta agtggcgttt gtgttggcct
     1201 ttgtgccacc agttttcaat ggtcgcttga gcttctgggc tgattgtttt gccttctagg
     1261 tagtcgtcaa tctctgcgta ggtgactcca agtgcgactt cgtcagctag gcctggttta
     1321 tcttcttcta ggtctgccgt tgggattttt tcataaaggg ctggctctgc gccaagtttc
     1381 tgcaagagct gttttccttg gcgtttattg aggcggtaaa gagggagaat atccgcaccg
     1441 ccgtcaccaa acttggtaaa gaaacctgtg atattttctg cggcgtggtc tgttccaatg
     1501 accgctccgc tatggaaacc agcaagggca tactgagcaa tcatacggca acgtgccttg
     1561 atattcccct tgttgaagtc tgaaacagga ctacctgtcg cttcaactgc agctgtcatg
     1621 gcatcagctg attccttgat attcacaacc aagctgacat ctggctggat gaaggctagg
     1681 gctttttgag catctgcttc atcagcttgc actccgtatg gcaggcggac agcgataaat
     1741 ttgtagctat cgtctcccgt ttcagctcgc agttcttcca tagctaattg cgccaaacgt
     1801 cctgccaagg ttgagtcttg tcccccagaa atccctagta caaaggtttt taggaaggga
     1861 tgttttttca gatatctttt taagaaatca atagaacgac ggatttcttc ctgggcatca
     1921 atcactggtt tgacacccag ctcttggata atcgtttctt gcaaactcat tcttcttctc
     1981 cttcaccaag ggcttccttg cgcatcttat caatcaagtc catcttatct tgccatacgt
     2041 cacgcgccaa atccactgga tagtgctgcg gattgagcac acgcttatac tcatcccaca
     2101 acttgtcaaa ttccttacgg gcataatcct gaatgtcagt caaactaggc aagttgtaaa
     2161 ctaatattcc ttctttgaag atatccacca agagaggaac agcatcaaaa ttacgaaccg
     2221 tcttcttgat gtatgtatag gtcggatgga acatcttgat ttctgtcatg tcgctaatat
     2281 ccacaccatc ataagtgatg tagtcacctt ctgacttgcc tttttcacga ctggtaatgc
     2341 gccacacctg cttcttacct ggcgtcgaca ctttttccgc attattagac agcttaatcg
     2401 tattgcgcat ctggccgttt tcatcttcga ttgcaacaat cttgtaaacc gccccaagag
     2461 ccggctggtc ataggctgta atcagcttgg tacccacacc ccagacatca atcttggcct
     2521 tttgcatctt gaggttaagg atggtatttt catctagatc attagaagca taaatcttag
     2581 cctctgtaaa tccagcctcg tccagttgct gacggacttt cttagaaatg taggcaatat
     2641 ccccagagtc aatccgcaca cccataaagt taatctgatc acccagctca cgcgccacct
     2701 gaatggcagc tggtacaccg atgcgaaggg tatcataggt atccacaaga aagacacaat
     2761 ttttgtgggt cgcagcgtaa gccttgaaag cctcatagtc attgccataa acctgtacca
     2821 aggcatgggc atgggttccc aaaacaggaa tgtcaaagag cttacccgca cgcacgttgc
     2881 tggttccatt ggcgccacca atcaccgctg cgcgtgttcc ccagatggcc gcatccattt
     2941 cttgagcccg acgtgtccca aactccatca agggttcatc ttcgataacc gaacgaatac
     3001 gagctgcctt cgtcgccacc aaggtctggt agttgacgat gttcaaaaga gccgtttcga
     3061 ccaactgaca ttgggctaga ggtccttcca cctgcacaat cggttcatta gcaaaaacca
     3121 aatccccttc ttgggcagaa cgaacggtca actccaactt gaaattgcga aggtaatcca
     3181 agaacgcccc atgataacca agcgactcca aataggctat atcactatct gaaaaacgca
     3241 agccttcaag atagttcaca attctttcca aacctgcaaa aaccgcatag ccgttcttaa
     3301 aaggctgttg gcggaaatac acctcaaaga ccgccttctt attgtgaatc ccttggtcaa
     3361 agtaaacctg catcatgttg atctggtaca agtccgtgtg caatgtcaaa ctatcatctg
     3421 gatacatact tttcctactt ccttagctag aaacccatga aaattttcaa gaactttcat
     3481 gtattccaat aaattagtac tattatatca cattttagct ggattgagaa aagagtaaca
     3541 agctattctc cactctccaa ttcatccata tcttgttcaa attttttctg agcccattcg
     3601 ccatagctct taagaccaag attgccaata aagacccacg gaaggtaaat gacataagta
     3661 atgacccaag cagacaggta tttaaaattc aaaggattgt gctgataaat ttctatgttg
     3721 aattgataat tctgcaacat caaaagagcc gtaatagcca aggttaggaa aaaacaaccc
     3781 aaaatcgtaa aatgaaaacg actatagtag gtcactccca gataacgggc acgattgaaa
     3841 aagtaaaatg tccctatgat gataacgatt agcagcatat tagaattaaa aaggcttggt
     3901 gctaatactg aaatgatata agataggagc gacaaagcga tgcagatata gagactttca
     3961 gagcccgctt tattgaacag ttgttcttct ctttcgtcta gtaattgata ataataaaat
     4021 ctatttttca tcttcttcct cccaaaatag ttggtctagg gttttcccta aacatctgca
     4081 aatagactgg cagagcgaga gactgggatt gtattttccc gcctctatca aaccaatagt
     4141 ctggcgtgtc accccgacag cctctgccag ttgaccttgt gttaaatcac gctctacccg
     4201 agctaatttt aattttaaat ttttagccac cttcgtcctc cttatagttt taatactcat
     4261 ctactcttaa aaaatccaaa accaacacaa gctatcagct atttattata accccattaa
     4321 tttgcactcc agtattatct tcagccgaaa atcagtattt taggttgttt gaaataaact
     4381 tcctagttta ctcttttatt tttatttagt attagcttta gtaggatctt ttaaacgagt
     4441 ttttattgct tatatttctt gtttagttta gttggtttca tatcactatt atataatgct
     4501 tttggaaatg agcctagtat aagtcaaaaa cgtgttgaat agtgaaagca ggagcatacc
     4561 aagatgactg attttttact gtattttagg tatgcaaaat aatactaaat gaaactaaaa
     4621 tgaatacttc ttgcttttct tgagagtgaa aaagtacaat aaaatgattg tagtgattag
     4681 cacaagatac tgactaatag acatcgtatt tgaagccaca aatagaggta aagaataaaa
     4741 aggaagaagt tcaattgctt tgataaacaa atcattacta gtattcatca tcatcgtatt
     4801 taatatatta gggataaaga gtagtaaagc tactaaaata ccaaatacca agccactttt
     4861 caatttgtat aaatagaaaa tgtaggataa tagaaaataa aacaggacta acaaaattgt
     4921 attgactaca atagctacta taaaatctaa tcctaaatca cctggaatac ccaatttact
     4981 tactatgact agtaacacaa tgccattcaa agcataaata atggaaactg agatggcata
     5041 tagaaaatta gacaagacgt aaatcatccg attcttctgt tgattataaa acaaacttac
     5101 cgtattgtgg gaaaaatctc tactaatgac tcgattagca tgtataacag ccattaacat
     5161 accgagcata gcataaaagt ttgaaatatg cttaatacca agcgatgtcc cttgagtctt
     5221 tagaataaag aatgtaagca atatcggcgg tataaacgag ataagcaaac tgagatatat
     5281 tacgcctgat gaata
//
LOCUS       DAVKWS010000087         4816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKWS010000087 DAVKWS010000000
VERSION     DAVKWS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.088"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     misc_feature    77..212
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            251..751
                     /locus_tag="VW852_001964"
     CDS             251..751
                     /locus_tag="VW852_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV0273041.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNNFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            827..1195
                     /gene="rplL"
                     /locus_tag="VW852_001965"
     CDS             827..1195
                     /gene="rplL"
                     /locus_tag="VW852_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV0273042.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            1397..1570
                     /locus_tag="VW852_001966"
     CDS             1397..1570
                     /locus_tag="VW852_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273043.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLILKELGGAL"
     gene            1567..2346
                     /locus_tag="VW852_001967"
     CDS             1567..2346
                     /locus_tag="VW852_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV0273044.1"
                     /translation="MKRITANQYHTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKILKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVANSNRPSVKKRLGQDENETAPVSHSAPSETEVSETKYSE
                     TDSLFIEDEEERYTQPILKRKVEKVTKYDQDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
     gene            2445..3803
                     /gene="dcm"
                     /locus_tag="VW852_001968"
     CDS             2445..3803
                     /gene="dcm"
                     /locus_tag="VW852_001968"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEV0273045.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDIRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGTRLLFPFRREGQATNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKVAIPCMTPDRLEKRQNGRRFKENQEPMFTLNTQDLHGIVVVGDLPTSFK
                     ETGRVYGSDGLSPTLTTMQGGDKIPKILIPEPIHFLKIREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLINGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     FK"
     gene            3787..4236
                     /locus_tag="VW852_001969"
     CDS             3787..4236
                     /locus_tag="VW852_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273046.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQIFLMPNYLCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDHQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKSINFSDLLDSLIVSEYQMEPNLWEVSHD"
     gene            4229..4609
                     /locus_tag="VW852_001970"
     CDS             4229..4609
                     /locus_tag="VW852_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273047.1"
                     /translation="MIDLYLSKNNHRNQILLDFFQNYGIEVSCHSISEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELSIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            4622..>4816
                     /locus_tag="VW852_001971"
     CDS             4622..>4816
                     /locus_tag="VW852_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0273048.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQ"
BASE COUNT         1630 a          747 c         1020 g         1419 t
ORIGIN      
        1 aaagaaaaat cacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt ctttttttga
       61 ctatgctata cttatatacg gtttaaaaaa actgcctaag acagtagggg agctcgactc
      121 ataaatatcc taccgaggac aagacgtatc atgtaaaaag aagcgtattg tactttcgtg
      181 tctaggtttg ggcgcgtttt tctttttgaa aaattcccca agcaaaataa ttacggaggt
      241 gaacacacta atgagtgaag caattattgc taaaaaagcg gaactagttg acgtagtagc
      301 tgaaaaaatg aaagctgctg catctatcgt cgttgtagac gctcgtggtt tgacagttga
      361 gcaagataca gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata aagttattaa
      421 aaactcaatc ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg catctgtatt
      481 tgttggacca tctgcagtag cattttctaa tgaagatgtt atcgcaccag cgaaaatctt
      541 gaacaacttt tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa tcgaaggcgc
      601 tgtcgcatct aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg aaggacttct
      661 ttctatgctc ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg cagtcaaagc
      721 ggttgcagaa agcaaagaag acgcggctta atcttaagct acacagcgta gcctagctac
      781 gaaaaaaact attataaaat ttaaaactta tttggaggaa ataacaatgg cattgaacat
      841 tgaaaacatt attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg accttgtaaa
      901 agctatcgaa gaagaatttg gtgtaactgc agctgctcct gtagctgttg ctgcagctga
      961 tgcagctgat gctggtgctg ctaaagattc attcgacgtt gaattgacat ctgcaggcga
     1021 caaaaaagtt ggcgttatca aagttgtacg tgaaattact ggtcttggtc ttaaagaagc
     1081 taaagaactt gttgacggtg caccagcact tgttaaagaa ggcgttgcaa ctgcagaagc
     1141 tgaagaaatc aaagctaaat tggaagaagc tggagcttca gttactctta aataagaggc
     1201 atataccaat tagaattcgg aatactatag tatcagcctt ttagaatcgt gaacacattt
     1261 tagaaactga taaattaatt tagtttccta ccaaaaaccc taccaaaaat taatttggca
     1321 gggtttttct ttttaacaaa tttatcgtgg agaacaattg aatattgttc tccttttttt
     1381 attttcagga gggaaaatga caaaagaatt acaatcatca cgctatattg tcatttcatt
     1441 tttagtacgt gaaatgggaa ttgatattgt tgaagccatc tctcttatgg cagaattaga
     1501 aaaaagtggc ttggttcgat tggaatcaag tggagattta atactcaaag aactaggagg
     1561 agctctatga aacgaattac cgcaaatcaa taccatactt cagaacggta ttataaatta
     1621 cctaaaattc tttttgagga tgaaaaatat atggatatga aactagaagt aaaggtggct
     1681 tattctattt taaaagatcg tttagaatta tctctcagtc gtggttggat agatgaagaa
     1741 ggggcggttt atttagtatt ttctaattct aaactgatga agttgttagg ttgttctaag
     1801 tcaaaattac tgtctatcaa aaaaattctt aaagaatatg acttaattga tgaagttcaa
     1861 cagtcttcaa gtgagaaagg gagacttgct aataagattt atttagggga attgtcttct
     1921 accccagtag ctaattcaaa caggcctagt gttaaaaaaa gactagggca ggatgaaaat
     1981 gaaacggccc ccgtctcaca ttcagcccct agtgagactg aagttagtga gactaaatat
     2041 agtgagactg attctttatt tattgaggat gaggaggaga ggtatactca acctatcttg
     2101 aaaagaaaag tagaaaaggt cacaaaatat gatcaagatt atatttgggg attggtacaa
     2161 gatcaattta gacgagaagg tttttctgaa acagccagtg aaattgctat gactgatttt
     2221 gagagaatct atcagtatgc tcttgacaat gttcgctttg ttagaagagc ggaagtactt
     2281 gctgaatttg tgtttaatgg cctgtattct gtttggaata accgtgttag aaaaggaggt
     2341 ggttagatgt cgtcaatcga gtggatatta gttttgctca ttttcatttc aagtggtgtg
     2401 acactttgga tattgatagt cgatcagaaa ggggtaatca agaaatgaga tttattgatt
     2461 tattttcagg tataggtggt tttcgacttg gaatggaaag tgtaggacac gagtgtattg
     2521 gattttgtga gattgataaa tttgctagag aatcttataa atccattttt caaacggaag
     2581 gagaaatcga atttcatgac atacgagatg tttcagatga cgaatttaaa aaacttagag
     2641 ggaaagtcga tatcatctgt gggggattcc cttgtcaagc attttcaatc gcaggaagac
     2701 gattgggatt tgaagatatt agaggaactt tgttcttcga aattgctcgg gcggccaaac
     2761 aaatccaacc acgttttctt tttcttgaaa atgttaaagg cttactcaat cacgataagg
     2821 gacggacgtt caccacaatc cttaccacac ttgatgagtt ggggtttgat gttgagtggc
     2881 aggtgcttaa cagtaaggat tttggcgttc cccaaaacag agagagggtg tttattatcg
     2941 gacattctag aaagaaaggt acccgactcc tatttccttt cagacgagaa ggtcaagcaa
     3001 ctaactctga gactttaaaa acactaggaa atttgaatcc atcaaaaagt ggcatgagcg
     3061 gtaaagtcta ttattcagag gggttagcgc caacattagt tcgtggaaaa ggagaaggat
     3121 ttaaagttgc gattccttgt atgacaccag acagattaga aaaaagacaa aatggtagac
     3181 gatttaagga aaaccaagag ccaatgttta ctttgaatac tcaggatctc catggtattg
     3241 ttgttgttgg agatttacca actagcttta aggagactgg tcgcgtatat ggaagtgatg
     3301 ggttatctcc aacactaact acgatgcaag gtggagataa aattcccaaa atactgatcc
     3361 cagaaccaat ccattttcta aaaatcaggg aagcaactaa aaaagggtac gctcaagcag
     3421 agattggaga ttcaatcaat ttagaaagac caagttctca gtatcggcgt ggtagagttg
     3481 gaaaaggtat agcgaatacg ttgacaacta gtggtcaaat gggagtagta gtggctagct
     3541 atgaaggaga agataagcaa gtttaccatg tagccggtgt tttaattaat ggacaatttt
     3601 accgtttgag aatacgacga atcactccta aagagtgttt tcgattgcag gggtttcctg
     3661 attgggcttt tgaagctgct agaaaagtct ctagtaatag tcagctctat aaacaagctg
     3721 gtaatagtgt aaccgttcct gtgattgctg caatcgcaaa gaaattaaaa gaaatagagg
     3781 aaaaagatga aagctttaaa taaagagtca atactagatt gtgacgaatt agaaacagaa
     3841 ttacatgatg cagaaatcaa acagctggat gaacaaatat ttttaatgcc caattatcta
     3901 tgtgagtttg aagtgacatt tttagatgat taccataaaa aacataacta tccactattt
     3961 tacgaatcct atcttcaaaa cgttatggaa ttccttgaaa gtcaagacat aaagaatggg
     4021 gttgatgcct ttgtagatga tcatcaaaat ctcgtttttg ttttatatgg acaaggctat
     4081 cgagccgagg gaaaagaggg aatacttaca acccaagtaa ctgtaaaagc ctatgatgaa
     4141 gacaagaaat caattaactt ctcagatcta ttagattcct taatcgtctc agaatatcaa
     4201 atggaaccga atctttggga ggtctcccat gattgatctc tatctaagta aaaataacca
     4261 tagaaatcaa attcttttag atttctttca aaactacgga atagaggtat cttgtcattc
     4321 gatttctgaa atgacaaagg acaaattaat tgagatgatg agctattctt cagattgttt
     4381 tgagttttta tctcctaatt tattacgatt taagaaccgt gacaatctaa gattaacgga
     4441 tttcatcgaa atgatattaa aaaatcctga actaagcatc agacttcctc ttgcagtttc
     4501 aaataagaga gtttatccaa atctgaattt ggaagaggct agagctctat tgccaagaga
     4561 tacgaaacaa ttgatttaca tggcacaaac acattattta tctaactaaa ggagaagcaa
     4621 tatggctgaa cgattttggg agaacttgtc cattatttta gctgaaagaa atattagctg
     4681 gattgagtta accagaaaaa tgtttgcggg agagtttcac tatccaagtg aattgaatcg
     4741 tttgtatcaa aagattcgtc actacaagat ggaacaacga atgcctcaaa gtccatgggt
     4801 agaaaggatt gtgcag
//
LOCUS       DAVKWS010000088         4816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKWS010000088 DAVKWS010000000
VERSION     DAVKWS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.089"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            129..1622
                     /locus_tag="VW852_001972"
     CDS             129..1622
                     /locus_tag="VW852_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0273049.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFRGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2177
                     /locus_tag="VW852_001973"
     CDS             1635..2177
                     /locus_tag="VW852_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0273050.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKFNELVKSRYSEEVVA"
     gene            2174..2761
                     /locus_tag="VW852_001974"
     CDS             2174..2761
                     /locus_tag="VW852_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0273051.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            2821..3057
                     /locus_tag="VW852_001975"
     CDS             2821..3057
                     /locus_tag="VW852_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV0273052.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            3054..3467
                     /locus_tag="VW852_001976"
     CDS             3054..3467
                     /locus_tag="VW852_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV0273053.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            3585..4550
                     /locus_tag="VW852_001977"
     CDS             3585..4550
                     /locus_tag="VW852_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0273054.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            4516..>4816
                     /locus_tag="VW852_001978"
     CDS             4516..>4816
                     /locus_tag="VW852_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0273055.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGENKIFESIDNLFDIIDGDRGKN
                     YPKSDELFSEEYCLFLNTKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTT"
BASE COUNT         1728 a          687 c          947 g         1454 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga ccagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcgtg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaagtcata ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attggaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt ttgtgagatt ttatctggtt
     1681 acgcttttaa atcatcacaa tttaatgata acaaaattgg tctcccctta atacgaattc
     1741 gagatgtaga aagaggtttt tctgatacct actttgaagg aacttatccc gaagaatatc
     1801 ttattaaaaa tggagactta ttgattacaa tggatggcag ttttatattg aaaaaatggg
     1861 agggggattt agcgctactt aatcaacgtg tctgtaaaat taaaattacg gataaaagtg
     1921 tagatgaagg ttatatatct tggttgattc ctaaattttt aaaagaaatt gaagataaga
     1981 ccccttttgt tacagtgaaa catctttcag tagctaaaat aaaagatatc tcatttgtat
     2041 tgcctaataa actagaacaa aagctaattg caaaaaaact aaatacgata agtcaaattt
     2101 atgattttcg taaaattcaa tcagaaaaat ttaacgagct cgtcaaatcc cgatattccg
     2161 aggaggtggt tgcatgaaga aagtgaaatt agggcaagtg gctactttta tcaatggcta
     2221 tgcttttaaa cctcaagatt ggtcatctga agggaaagag attatcagaa tacagaatct
     2281 gactaaaact tcaaaaggga taaattatta ttcgggtacg attgataaaa aatacattgt
     2341 tgaagctggt gatattctta tttcgtggtc gggtacatta ggggtttttc agtggtgtgg
     2401 aagatccgca gtgttaaatc agcacatttt taaagtggtt tttgataaga tagatataga
     2461 taaatcgtat tttaaatatg ttgttgaaaa aggtttacaa gatgctgtaa aacatactca
     2521 tggttccaca atgaaacatt taactaaaaa atattttgat aatattatgg tttcatatac
     2581 aaatttggga gagcaacaaa gaattgcaag tgagctggat ttgttaagca agctgatttt
     2641 aaggagacaa gaacaacttg aagagcttaa tctacttgta aaatcacaat tggcaatcca
     2701 aaaatctctg gaagaacttg aaactttgaa gaaatctctg atgcaggagt attttggctg
     2761 atattctgcc attgtaatta cggtaatgat ttgttataat acttcaaagg aggaaatcag
     2821 atggtagtaa aaacaagaaa acaaggaaat tcaatcacca ttacgattcc aagtgaattt
     2881 aatattccaa gtggtgttaa atacgaagcg aaattgttac caagtggtga gattatcttt
     2941 actcctgaag aattggggca gcaggtttct tatgtatctg atgatgcctt tgacttaaat
     3001 ttagataaaa tatttgacga atacgacgat gttttcaaag ctttggtgga aaaatgacaa
     3061 tctatctgac agaaaagcaa attgaaaaaa taaatgcttt agcaattcaa cggtattctc
     3121 caaatgagaa aattcaaaca gttagttctt ctgccttaaa tatgattgtg aacttaccag
     3181 aacaatttgt ctttgggaag tctctttatc caacaatttt tgataaagca acgatactat
     3241 ttgtccaatt gataaagaag catgtttttg ctaatgctaa taaaagaact gctttcttcg
     3301 ttttggtcaa atttttacaa ttaaacggct atcgtttttc tgtaacggta gaagaagcag
     3361 taaaaatgtg tgtaaccatc gcagtagaag ctttaactga tgaaaaaatg acaagctact
     3421 ccaaatgggt ttctgaacat tctgttagag aaaaggtcaa aaagtaacct agtatgctgg
     3481 atttgaatga gcacaagaaa ataaatgaac agacaatatt agaattctgt aatgcagaaa
     3541 ctgatattgt ctctttttat tgatgaataa gaaagtgaga aattatggaa tcaaaagtta
     3601 caattatcat gcaagaaatg ttacctcttt taaataatga acaattacta gcgttgagag
     3661 agagtttaga acatcatcta gtagacggaa aaaagcagca gaagtattcg aataataacc
     3721 tgttgcaact atttattacc gccaagcagg tagagggctg tagctcaaaa acaattcgtt
     3781 attaccagag gacgattgaa aacttgttta atgctattaa agagtctgtg acacaactca
     3841 caacagatga tttaaggagt tatttagcaa attaccagtc tgaaaaggat tgtagtaagg
     3901 caaatttaga caatattagg cgtatattgt cttctttttt tgcttggctt gagcaagagg
     3961 aatatatcat taaaaatccc attcgacgga taaagaaaat taagactgag caaaatgtga
     4021 aggaaactta tactgatgaa catttggaaa ttatgcgtga taactgtgaa aatttgagag
     4081 atttggcaat aatagaccta ctagcatcga caggtatgcg tgtaggggag cttgtacagt
     4141 tgaatcgttc agatattgat tttgaaaaca gagagtgtgt tgtctttggt aaaggaaaga
     4201 aggagagacc agtatatttt gatgctcgga cgaaaattca tttaagaaat tatcttaacg
     4261 acagaaaaga tagtcaccct gctctttttg taacgctagt tggaaaagcc cagagacttg
     4321 gaattgctgg tgtagagatt cgcttaagaa agttaggaga caaactcggc atacaaaagg
     4381 ttcacccaca taagttcaga agaactttag cgactaaggc aattgataaa ggtatgccta
     4441 tcgaacaagt ccaaaaactg ctaggtcata gcaagattga cacaaccctg gcctatgcca
     4501 tggtcaatca aaataatgtc aagcattcac accaaaaatt catctcttaa aagcaaatcc
     4561 cgatttaacg agatgtttgg ggaaaataaa atatttgaaa gcattgataa cttatttgat
     4621 attatagatg gtgatagggg caaaaattat cctaaatcag atgagttgtt tagtgaggag
     4681 tactgtttat ttttaaatac aaagaatgtt actaaaaacg gattttcatt cgatacaaag
     4741 caatttatca ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga gcgttatgat
     4801 atagtcttga caacaa
//
LOCUS       DAVKWS010000089         4737 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKWS010000089 DAVKWS010000000
VERSION     DAVKWS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.090"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(93..1319)
                     /locus_tag="VW852_001979"
     CDS             complement(93..1319)
                     /locus_tag="VW852_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0273056.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1391..2056)
                     /locus_tag="VW852_001980"
     CDS             complement(1391..2056)
                     /locus_tag="VW852_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0273057.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQYAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTVLEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2068..2745)
                     /locus_tag="VW852_001981"
     CDS             complement(2068..2745)
                     /locus_tag="VW852_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0273058.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWFYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            complement(2767..3000)
                     /locus_tag="VW852_001982"
     CDS             complement(2767..3000)
                     /locus_tag="VW852_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273059.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            complement(3291..4154)
                     /locus_tag="VW852_001983"
     CDS             complement(3291..4154)
                     /locus_tag="VW852_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0273060.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVYNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
     gene            complement(4549..>4737)
                     /locus_tag="VW852_001984"
     CDS             complement(4549..>4737)
                     /locus_tag="VW852_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV0273061.1"
                     /translation="LIVGIKILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDR
                     GLEAYKDKVYTRGIWIRD"
BASE COUNT         1659 a          935 c          803 g         1340 t
ORIGIN      
        1 cacattcaga aaagagaaaa aagtctgttg attttgacca tcataaaaag actggcaatc
       61 cagtctcaaa catatattat agaaattctc cactaaatac tttcacgaat attcagaagc
      121 ataacaaagg caactagaag aaatagcaat aaaacaaagc taactgccag agttccaaag
      181 ctagtagcaa tggttaccaa agatattgta aataagctag gtaaaacaac cgtaatggca
      241 ctgatagaag attgaactgc tcccattgac tcctcaggta tttgtttaaa gacgagttct
      301 tgcaatctag gagagagaac acctgcgaaa aaggcatcca aggtactaaa gatgagaatc
      361 cagtcaaaac gaactgtggc aaatcctact agaagaagca actggatgac aagtgaggca
      421 tagagagctg tttttatgga aatggtatgt tgcagatagc cacttacaag gcttccgaca
      481 atcagggctg acagttcaaa agtggatagc aaggcaagag attgacctgt ttgtaaattc
      541 aaaaagggtt gattcctcaa aaatagagta gagacaggaa ctgtaacatt tatcactgct
      601 tgactagtag agataataaa caaaaccaag agcaccttat tcatattcca tatcaatttc
      661 gatgattgga gcaaatgctg gcaaaaggat tttacagaga gtccttcttg atagttaatc
      721 gttttttcta ctttcaagag gtcagttttt atgaagagga tacctaaaaa tgcgattaaa
      781 aaggtaagag cgttcagcaa ggaaataaac tggatggata gaatacctaa taagactcct
      841 cctagaatat tactgattgt tttcactaaa ctaacagttg actgtttaaa tccaatagct
      901 tctgccagat ggtcttgccc aataattcta atgaaaatcg gagtgagcat ggcgcctgaa
      961 aaataactca acgtgtcaga caagaggtta atcagacaaa taaatgctac taataacaag
     1021 gaaaaggact gccctgaaag tgataaagac actatagagt aaagcaaaaa ttttgcaaaa
     1081 ctaatgactg tgtatttcaa gacacgatga tgttgaaaat ccgccaaaac tcccagaaag
     1141 atttgtagaa cttggggcag ggtttctgaa atcgtgatga gtaaaatcgc caaaggggca
     1201 aaagatgcat ctgccacata attcaggaag gccagataaa aaatcgtatc cccaagcgtt
     1261 gaaatccact ggttgatagt taattgccta aaatctctat tttgaagaaa tactttcatc
     1321 acaactcctt cttaagttca aatgggaagc tttccccaag gatagaccgc gatactacta
     1381 acaaccaaaa ttacagtaac atcaaaagct gaccaatgcc attgtagact atatgcagtc
     1441 caataggcca ataaattgac tttgtcattc taaataagac tgcaaatata agacctccac
     1501 ccatatagaa gacaaagtct gtcaagaccc aaccgtgatt actaatgtgc gagaccccaa
     1561 ataaaacagc ggaaccaagt acatctagcc cccatttctt tcctttttcc agaactgtca
     1621 tcaccaatcc ccgatggatt atctcttcaa aaatgggacc tgcaatcaca ggatagaaaa
     1681 aatacatcaa aaatgctgta gcccctgtaa aagtcggagc agcatattga taagaaattt
     1741 catttcgagt aggtgggaaa agaaaaaagg taacgaaatt ccaaacaaca aaagcaagca
     1801 gagctaggaa ggaatagaaa agataggatc ctttaaactt tctactattg attttctgcc
     1861 atttccccga ccaaatcata gcaataagag caaataaaac cacaagaaaa ttcaacatca
     1921 tatccgacag ataataggca aagtcagata gcccagtaac aaggtcgctg cgtaaaacta
     1981 gaacactgaa cttctggtca gcaataacta gtagaaaaac tataataaag tagcggtgtg
     2041 agattatctt tttcatatat cacctttcta atatccaaat accaataaag taacaatgag
     2101 taagaaacta ttccatgaag catgcagagc tatagcccaa tagatggatc gggtgtagcg
     2161 aaacatcata caaaatatca agcccattcc aaaatacttt atgaaatctg tcgttatcca
     2221 accatactgc aaaacatgca tagcgccaaa tatggcagcg gaaacaagaa catcaagata
     2281 gtatctctta actttagata aacttgtcat caaaagacca cgacaaacaa cctcttctga
     2341 tacaggtgcg ataatactag tataaagtat tcgcgtaaca aaatagctaa ttcctgttaa
     2401 attggtggct acttctacga ctgtacttcc attctgggta cgaggaaaga tataggttgt
     2461 tagatttgcc cacacgaaca ataagaaaaa agaaagaagg aaaacaccca ggtaaaacca
     2521 acgaaactgg aaacgaccac actctttcca atgttcactt ttgacaaaag caattgtagc
     2581 tatagttccc agaataagta ccaataaaac ttggaacaca tagtacatat tatcagacaa
     2641 agcaaccata aaatctaagt ctgatgtgac attaaaaatg aggtaataag tcaaaatcaa
     2701 caagccagtt gctaggtgaa atttcacttc tttcattttc ttcatcctat tatctcctat
     2761 aagagcctat cttctacggc ggccaaacaa tccatctgct aaatctatag tccaatcaaa
     2821 agctccacga ttaggactca tcccttgatt gccccaacca gggtaaattc ctgggacgcc
     2881 ccaaccagat ataccacttc ttccaccacc tcccatagaa tttacgaggt tgcctcctct
     2941 aacatcttgc aactcagctt ctgtcaattc cattgtttct gcaaattgta aatttaacat
     3001 cttttacact ccttcaatta tcttcatttg taaaccactt ctgcgaccta ggatttgctt
     3061 caagtgcttt acaagtacag tataacacga aaattggctt attttaaaaa atcgcatatt
     3121 tgatattttt tcttatagaa atttcttatt tgcgattttg atagatttga ttatttccct
     3181 ggtataataa agttattact aacaaggagg aatataatag atgaagaaaa gaaaaatcca
     3241 actgatactc ctacttattt cggagtgggt tattgtcatt ccatttttaa ctaatctata
     3301 agttctttat attgctgaaa acgcaattca aaaagggatg ttaattgtgg attttctaat
     3361 acctgcagag attggataaa gcgttcaatc tctttttgat tgcttccctt tgtttgaaga
     3421 aagacactca tcttctttaa aaattgccac gatacttttt caaaaacatc atacggtcgt
     3481 aacatcctct ccaactcggc ttcgaagatt gggatgtagg agaaaagttt tcgctccatg
     3541 agttctgata agatatttaa gagtccttgc ttcatataca atcgattgta tactaactct
     3601 ttaaattctt tggatttttc gagtaaggag gttgataaaa aaatcagatc ttgattgctc
     3661 aagaagggca tggtattgca aaagagatag agttcaaacc aggtccaaga ctcgatagca
     3721 tagagatagg tggtcaaaaa ctcgctatcc tcctctgcta gtgggtagct tttatttagt
     3781 gaatggatgg catctttaat cacgatggca ttcaaacgac gataggtctg cgccatctgt
     3841 tcttgatcga cttcctccaa tagctgctct aaagcagcta tatcctgatg ggcaaagcga
     3901 ttcacaacct ttcgaccgat tcgcatatgt ggagattctt gatagttgtt gagcttgtgc
     3961 ccaaactcat caaaggtcac atttatacct tggatagcta gaatcaactt atccgcagac
     4021 agcatagact gccctagttc aaacttggac aactgagaag ctgttagacc ctcacaagcc
     4081 acatctgtct gcttaagctt tctcgccaaa cgtaattcct tgtaaaattc ccccagttcc
     4141 attttttcaa tcatcctacc acctcctagc ttttgcatat tatatcatta tttgtgttca
     4201 tttgtcaaaa cattctgaca ctttggtgat tgaaaaagcc agccttaagc tgactctaaa
     4261 aggaccgagc cttgtacaat acagaactca atcctcgttc taaatagaat gtctaacttt
     4321 tttgggcagt acacgagctt ggggaacagc cactggaacg atgaggtgtg agctcaaaat
     4381 atcctccagt tatgtttttc ctaatagtat accggaagag tgaaaggatt ttataatgga
     4441 gcggttacaa agaacctact ttctattaaa cagtatacta tgaaaatgtg aaaatttaac
     4501 atttttttgt acaaatttta taaattattt cctttttaat atcaatagtt aatctcttat
     4561 ccagatcccc cttgtgtaaa ctttatcttt ataagcttca aggcccctat cccatctatt
     4621 tgcaacaatt agatcacttt gttttgtaaa tagttcaaaa ttcttttcaa aaattacgtt
     4681 atctatacta acgtttaaat ttggttcata tactaaaatt tttataccga caatcaa
//
LOCUS       DAVKWS010000090         4701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKWS010000090 DAVKWS010000000
VERSION     DAVKWS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.091"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            107..904
                     /locus_tag="VW852_001985"
     CDS             107..904
                     /locus_tag="VW852_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV0273062.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1052..2311
                     /locus_tag="VW852_001986"
                     /pseudo
     CDS             1052..2311
                     /locus_tag="VW852_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2407..3681
                     /locus_tag="VW852_001987"
     CDS             2407..3681
                     /locus_tag="VW852_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV0273063.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTTEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3693..>4701
                     /locus_tag="VW852_001988"
     CDS             3693..>4701
                     /locus_tag="VW852_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV0273064.1"
                     /translation="MEDDLNYENLMDDVTEAIKKFNLVIFIGAGVSIAQGYPNWNNYI
                     EHLIKYWQGQVLSVSGEKRLGREHHVVFDLISKSSISNKRKVDLVNYELKKVFGEDFE
                     KRRLDFEKGYFKNLLPYSIVNQTVESLASLNAIFITSNYDYEIENHIKRLKNAVVTIN
                     DLNEFTKNKNGKLQFGDVLHIHGTPDCDVKYFVSSSADYSKTYLKNRENFENLVTWFK
                     ETKPTVLFIGAGLEEDEILSLLCKDSKNYALMKSENTGNQRVDEHYRGVVEGFFSSEN
                     HTQIIWYGDEFEKLPLFVKKLVADINEKLGTHDFYNQWNNLLNPSINQEEYNKNLDSI
                     SN"
BASE COUNT         1509 a          792 c          985 g         1415 t
ORIGIN      
        1 tattctagtt tcaatctact ataaactttt atgaaataca gaaaacgctt cctaaaacct
       61 aaagtttgtg atataattat caagaaaaaa gatttaggag tttattatga tttcttcaga
      121 atttatctca aagattgaat ttgcttgcaa taagaaagaa agtctttata gtcaaagcaa
      181 atttaagtat gcgattcgtt cgatgttcgc aggtgcattt ttaaccttca gtactgctgc
      241 aggtgcagtt ggggctgact tgattaataa aattgcacca ggtagtggac gcttcctctt
      301 tccattcgtt tttgcttggg gcttggccta cattgttttt ttgaatgccg agttggtcac
      361 ttcaaacatg atgttcttga ctgctggtag tttcttaaaa aaaatctctt ggagaaaaac
      421 agctgagatt ttactatact gtaccttgtt caaccttatc ggagccttga tagcagggtg
      481 gggctttgct cattcggcag cctatgcgaa tctgacacac gatagtttca tctcaggtgt
      541 tgttgagatg aagttaggcc gctcaaatga attggtcttg cttgaggcga ttttggcaaa
      601 tattttcgta aatattgcga ttctgtcatt tattttggtc aaagatggtg gtgccaaact
      661 ttggcttgtg ttgtcagcta tttacatgtt tgtattctta acaaacgagc acattgcggc
      721 gaactttgct tctttcgcga ttgtgaaatt cagtgttgct gcggattcaa ttgccaactt
      781 cggtgttgga aatatgcttc gccactgggg tgtgactttc atcggaaact ttatcggagg
      841 aggcctcttg atgggtcttc catatgcctt cctcaataaa aacgaagata cttatgtaga
      901 ttaagaaaat gagcacgatt gagtcgtgct tttttcattt tcaaaataag gtaatagcta
      961 tttcttatat caaaatatag aaaactgata tttgtaaact ataactcaag gtgctacaat
     1021 atccttaata aaatgatatg gaggtcacct tatgacttgt gattttaaat ttgaaactct
     1081 acaactacat gctggtcaag ttgtggctcc agctactaag tctcgtgcag tgccgattta
     1141 tcaaacaaca ttttttgttt ttgatgacac gtaggaaggt gccgatctgt ttgccttgag
     1201 gaaaccaggg aacatttata ctcgtatcac caatcctaca acagctgccc ttgaaggtgg
     1261 tgttgaagcg ctagcaacag catcaggtat gactgcagtg acttatacga ttttggcgct
     1321 tgcccatgct ggtgagcatg tagtggctgc ttcgactatt tacggtggaa ccttcaatct
     1381 tttgaaaaaa ccccttcctc gttatggtat cacaacaacc tttgtcgatg ttgataattt
     1441 ggaggaagta gaagcagcta tcaaagacaa taccaagctt gtcttgattg aaaccttggg
     1501 taaccccttg attaatattc cagacctgga aaaactggca gagattgctc ataaacatca
     1561 aatcccactt gtgtcagaca atacttttgc aacaccttat ttgattaacg tcttctctca
     1621 tggcgttgac attgccattc actctgcgac taagtttatc ggtgggcatg gtacaactat
     1681 tggaggaata attgtcgata gtggtcgttt tgactggacg gcttcaggga aattccctca
     1741 atttgttgac gagagtccaa gctgccacaa tttgagctat actcatgatg tgggtgcagc
     1801 agcctttatt atagctgttc gagttcaatt gcttcgtgat acaggtgcag ccttgtcacc
     1861 attcaatgct ttcctcttgc tacaaagact tgaaaccctt tcacttcgtg tggaacgcca
     1921 tgtacaaaat gctgagacaa ttgttgattt tcttgtcaac catcctaagg tagaaaaggt
     1981 aaattatcca aaacttgcag atagtcctta ttatgccttg gctgagaaat acttgccaaa
     2041 aggtgtcggt tcaatcttta ccttccacgt caaaggcgac gaggaggaag cacgcaaggt
     2101 cattgataat ttagaaatct tttctgacct tgcaaacgca gcagatgcta aatcgcttgt
     2161 tgtccatcca gcaacaatca ctcacggtca attgtcagaa aaagacctag aagcagcagg
     2221 tgtcacacca aatcaaatcc acttgtctat cggtcttgaa aatgtagaag atttgattga
     2281 agacttacgc ttggccttgg aaaaaattta aagtaaaaga agataaacag tgggttttga
     2341 ctcactgttt ttgattttcc ctcaggcatg atataatggt tacagaagtc tagaaagagg
     2401 aatgtaatga acgaaatcaa atgtcccaac tgtggggaag tctttacagt aaatgaaagc
     2461 cagtatgctg aacttttgtc ccaagtgaga acgacagagt ttgataagga actacacgat
     2521 aggatgaggc aggaactggc cttggctgag caaaaggcta tgaatgagca acagactaaa
     2581 ctggctcaga aggatcaaga aattgcgcaa ttgcagagtc aaatccaaaa ctttgataca
     2641 gaaaaagaat tggccaagaa agaagttgaa cagacaagcc atcaggcctt attggctaag
     2701 gacaaggaag tacaggcctt ggaaaaccag ttggcgacct tgcgtttgga gcatgaaaat
     2761 caattgcaaa agaccctttc tgacctagaa aaagaacgca atcaggtcaa aaatcagctc
     2821 ctactgcaag aaaaggaaaa tgagttgtct ttggcttctg ttaagcaaaa ctacgaagcc
     2881 cagctcaaag cagccagtga acaagtcgag ttttataaga attttaaggc tcaacaatct
     2941 acaaaagcga ttggggaaag cctagaacag tatgcagaga gtgagtttaa caaggttcgt
     3001 agtttcgcct ttccaaatgc ttactttgag aaggataaca aggtctcttc gcgtgggtct
     3061 aaaggggact ttatcttccg tgagtgtgat gaaaatggag ttgaaatcat ttctatcatg
     3121 tttgagatga aaaacgaagc ggacggaaca gagaagaagc acaagaatgc agatttttac
     3181 aaggaattgg acaaggaccg tcgggagaag aactgtgagt atgccgtttt ggtgaccatg
     3241 cttgaggctg ataatgacta ctttaacaca gggattgttg acgtcagtca cgagtatgaa
     3301 aaaatgtatg ttgttcgtcc tcaattcttt atccaattga ttggtctctt acgtaatgcg
     3361 gcgctaaatt ccctaaaata caagcaggag ttggccttgg ttcgcgagca aaatattgac
     3421 attacgcatt ttgaggaaga cttggatgcc tttaagctag cttttgctaa gaactataat
     3481 tcagcttcga ctaactttgg aaaagctatt gatgaaatcg acaaggccat caaacgcatg
     3541 gaagaggtta agaaattcct gactacatct gaaaaccaac tccgtctagc taacaacaaa
     3601 ttggaagatg tctctgttaa aaaattgacc cggaaaaatc caacaatgaa agcgaagttc
     3661 gaagcactga agggggagtg aggaaataag ttatggaaga tgatcttaat tatgaaaatt
     3721 tgatggatga tgtaaccgag gctataaaaa agtttaactt agtaattttt attggcgcag
     3781 gagtttcaat tgctcaaggt tatcctaatt ggaacaatta tattgaacat cttattaagt
     3841 attggcaagg acaagtttta tcggtaagtg gtgaaaaaag gcttggaaga gaacatcacg
     3901 tagtttttga tttaattagc aaatctagta ttagtaataa acgtaaagtg gatcttgtaa
     3961 actatgaatt aaaaaaagtt tttggtgaag attttgaaaa gcgacgacta gattttgaaa
     4021 aaggatattt taaaaatcta ttaccatatt caatagttaa tcaaacagta gaatctttag
     4081 caagtttgaa tgcaatattt attacttcta actacgacta tgaaattgaa aatcatatta
     4141 aacgtttaaa gaatgctgta gttactatta atgatctcaa tgaattcact aagaacaaga
     4201 atgggaaact acaatttggt gatgttctac atatccatgg aacaccagat tgtgatgtaa
     4261 agtattttgt aagttcatcc gcagattatt caaaaaccta tttaaaaaat agggaaaatt
     4321 ttgaaaattt agtaacatgg tttaaagaga caaaacctac tgttttattt attggagcag
     4381 gtcttgaaga agatgaaatt ttatctttac tttgtaaaga tagtaagaac tatgccttga
     4441 tgaagtcaga gaacacaggt aatcagagag ttgatgaaca ttatagaggt gttgttgaag
     4501 gattttttag ctcagaaaat catactcaga ttatttggta cggagatgaa tttgagaaac
     4561 tacctctttt tgtaaaaaaa ttagttgctg atattaatga aaaattgggc acacatgatt
     4621 tttataatca gtggaacaat cttttgaatc cttctataaa tcaagaagag tataataaaa
     4681 atctagatag tatttctaat g
//
LOCUS       DAVKWS010000091         4688 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKWS010000091 DAVKWS010000000
VERSION     DAVKWS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.092"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..388
                     /locus_tag="VW852_001989"
     CDS             <1..388
                     /locus_tag="VW852_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV0273065.1"
                     /translation="ELLEMLRDSGLNIMYMPIIKTIEELDFVLSFEGINIVAVELVFQ
                     DLENPIISKKVIDDLHEKGLLIWVNALTLSDSIILSAKIDDDTAIAHDGESWGKLVSI
                     GFDIIQTDWPLLLYQYLVEKNKKIKK"
     gene            423..1484
                     /locus_tag="VW852_001990"
     CDS             423..1484
                     /locus_tag="VW852_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0273066.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNELKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            1501..2505
                     /locus_tag="VW852_001991"
     CDS             1501..2505
                     /locus_tag="VW852_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0273067.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSETQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIEEKVTMYINPRDIMQF
                     "
     gene            2517..4211
                     /locus_tag="VW852_001992"
     CDS             2517..4211
                     /locus_tag="VW852_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0273068.1"
                     /translation="MRKSLNWKDWLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLILFNLVLVGTVIILLIAYTIQKLPFSYRMIR
                     AVFFSIDNDMEEAARSMGASSFYTMVRVIIPYILPVVLSVVVLNFNSLLSDYDLSVFL
                     YHPLFQPLGIVIKQSTDETATLNAQAMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
     gene            4213..>4688
                     /locus_tag="VW852_001993"
     CDS             4213..>4688
                     /locus_tag="VW852_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV0273069.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSL"
BASE COUNT         1581 a          660 c          867 g         1580 t
ORIGIN      
        1 agaattattg gaaatgctta gagactctgg tttgaatatt atgtatatgc caattatcaa
       61 aacaattgaa gagttagatt ttgttctctc atttgaagga ataaatatag tagctgtcga
      121 acttgtattc caagatttag aaaatccaat cataagtaag aaagtaattg atgatttaca
      181 tgaaaaagga ttacttattt gggttaatgc tttaacctta agtgattcaa ttattctttc
      241 ggctaagatt gatgatgata cagctattgc tcatgatggc gaatcttggg ggaaattggt
      301 ttcaatagga tttgatatta ttcaaacaga ttggccatta ttactttatc aatatctagt
      361 tgaaaaaaat aaaaaaataa aaaaataaaa aaataaaaaa ataaaaataa aggagaacta
      421 tcatgttcaa aaactttaaa aagattgcat tcacttcttc actaatttta cttgctgctt
      481 gctcaaacaa tgcttcaaat acaaatcagt ctactgatga aggaagtgtt gataaatctc
      541 aagaaattgt aatctataca aattcggctt ctaatggacg tgcagattgg ttacaaacca
      601 aagcatcaga gcaaggatac aatcttcgtt tggtagatat ttcaggtgga gaactagctg
      661 accgtttgat tgcagaaaag aataatgctg tggctgatat ggtaattgga ttaaataaat
      721 tagaatttaa ccgtattaaa gctgaaaaac ttcttgtaaa atactcacca aaatgggctg
      781 atgaagtaga tagctctttg ggagattctg aagggtatta ttcaccaatt gtaaatcaac
      841 cattagtttt gattggtaat aaagatgcta aaatgccatc cgactggaca gattttactg
      901 attcatctta taaaggtaaa tatggtattt caaaattaag tacaggtaca tctaagaaca
      961 tctttgcaag tattgtttcg agatataaag atgaaaaagg tgagttagga atatctgatg
     1021 aaggttggaa agttgcaaaa gcttacctta aaaatgcaca cgtttatgca gaaggtgaag
     1081 actatatctc ttcaattatg gataataata atgagctaaa atatagtatg atgtggggat
     1141 ctggtgttct acaaaatcaa aaagaacgtg aatataaatt ccaagtaatg tcaccaaaag
     1201 ttggtgttcc ttatgtaact gaacaagttg ggattttaaa tacaagtaag aagcaagcat
     1261 tattaaaaga atttgtagat tggtttggct cttcagaact tcaaaaagaa tggtctgata
     1321 aatttggttc aattccagct aataaaaaag ctctagagca agcaaaagat gaactcaaac
     1381 agtttgctaa tagtgttaaa ccacaagaac ttgactggga gtttatcgga aaaaacatcg
     1441 attcttggat tgaaaaagca cagcttgaat ttgttgaaca gtaatagtta ggattaagtg
     1501 atgattacat ttaaggatat tcaaattaag tataatgatt ttattgccat cgataatctc
     1561 aatttaacaa tcaatgaagg agagtttttc acatttttag gaccaagtgg ttgtgggaaa
     1621 tcaacaactc ttcgtgcact agttggattt ttggagcctt cacaggggca aatactagtt
     1681 gataatcaag atattacgaa tttagatccg gaaaaacgcg aaattggtat tgtgtttcaa
     1741 agttatgcat tattcccaac aatgactgtt tttgataata ttgcttttgg tctaaaagtt
     1801 aaaaaagttt ctcctgaaat tattaaagct aaagttcatg aaattgcgaa aaaaattaat
     1861 atctcagacg agcaactaaa ccgaaatatt tctgaattat cagggggaca acaacagcgt
     1921 gtagctttag cacgtgcatt agtattagaa ccaaaaattc tttgtttgga tgaaccgtta
     1981 tcaaacctag atgccaaact acgagtttcg ttgagaaaag aattgaaacg attgcaaaaa
     2041 gaactaggaa tcactactct ttatgtcaca catgatcaag aagaagcatt aacattatca
     2101 gataggattg ctgtgtttaa taatggattt attgagcaag ttggcacgcc gtatgatatt
     2161 tataatcaat ccgaaactca atttgtctgt gattttattg gctcaatcaa taaaattagc
     2221 caagaagttc ttaaaaaatt acaaggcgag aataaatact ttaaagaaac tgaaggatac
     2281 atacgcttag agcgtgttaa gttagagaaa acttcacctc aagatttatc tataacagga
     2341 gtaattagtg atattgagtt taatggtgta cttattaagt ataaagtaga cttgtcgaat
     2401 agtgaaagta ttcatattat cgaaaagaat gatggactat cacgtcatat aatcgaagaa
     2461 aaagtcacta tgtacattaa tccgcgtgat ataatgcaat tttaaggaga ttgtgcatga
     2521 gaaagagtct aaactggaaa gattggctca taagattagg agttttatgg ctaatctttg
     2581 ccctgattat ttatccaaat attgaacttt tgtctggagt gttttataaa aatggttcat
     2641 ttactctaga ggcattcaat aagattatac actctcagag agcgatgagt agtatattta
     2701 atagctttat tttggcgatt tcaatggtct ttactgtaaa tattgttggg acattgattg
     2761 ttctactcac agagtactgg gatattaggg gttcaaaaat acttaaatta ggttatatga
     2821 gctctctagt atatggagga attgtattag cgacaggata taaatttgtt tatggcgcta
     2881 atggtcttgt aactaaaacg ctctcaagta taattccagg tcttgcccct aactggtttc
     2941 aaggatttgg tgcagtattg tttattatga catttgcttg tacctcaaat cacataatgt
     3001 ttttaacaaa tgcagttcgt agtgttgatt atcatacaat tgaagcagct aaaaatatgg
     3061 gtgcaagtcc tttaaaagtt ttctttagtg tggtttttcc aacttttaaa cctacgttat
     3121 ttgccttaac catcttaact tttttgacag gtctttcagc agtttctgct cctttgatag
     3181 taggggggaa agattttcaa actattaacc cattgattat cagtttcgct aaaactacaa
     3241 gttcgagaga tttagcagct ttactagcta tcatgttagg tttagcaact attctattat
     3301 tgacactttt gaatcatgtc gaaaaaggag gtaattatgt atctgtttcg aaaaccaagg
     3361 ctagtattaa gaagcagata atttcttctc cattatggaa tactcttgct cacattgttg
     3421 cgtatgtatt gtttgcaatc tatatgttac caatagtttt gataattcta tactcgttta
     3481 cagactcgtt gacgatcaag acgggaactt tagatctatc taaatttacg ttagcgaact
     3541 atgctaattt atttacagat gctcaatcat ttcgtcctta cctagtcagc gttgtttatg
     3601 caattttagc tgcggtaatt gctacaatta tagcaatagt aatttcacgt attgtacata
     3661 aaaacaagag taagttcgat aaattctttg agtatggtgc actaatacca tggatattgc
     3721 caggtacatt gattgcacta ggattaatgt tcacttataa tatacctcat ttaatactat
     3781 ttaatttagt tttagtagga acagtaatta ttttacttat tgcatatact attcaaaaat
     3841 taccattttc ttatagaatg attcgggctg tatttttcag tatagataat gatatggaag
     3901 aagcagcacg tagtatgggt gcatctagtt tctacactat ggtgcgagtt attataccgt
     3961 atattttacc agtcgtatta tcagtagttg tgttaaactt taattcttta ttatctgact
     4021 atgacttatc tgtattcttg taccatccac ttttccaacc attgggaatc gttattaaac
     4081 agtctactga tgaaacagca actttaaatg cacaggcaat gatgtttgtt tattctgtaa
     4141 ttttaatgat tatgtctagt gcagctcttt atttatcaag cctattccaa ggtaaacgag
     4201 gtaagagata atatgaatct ttcatacgaa ctgagtattt gggaaatact tcttaggtgc
     4261 tttttagcga ttcttttcgg tggcattgtt ggctatgagc gaggagtcaa aaaccaagca
     4321 gcagggatta gaacctactt actagtttgt ttgggaactt gtgttgtaat gatgacaaat
     4381 cagttcatca ctagtcgtta tggtagcggt gatcctagtc gaatgggagc ccaagtaatt
     4441 agtggcttgg gtttcctagg ggcaggaaca atcatggtaa ctaatcaaaa agttaaaggt
     4501 ttaacaacag cagctggatt atggtcttca gcaactattg gtttagctat aggcattggt
     4561 ttttatgaag gtgctatttt agccggagtt gtactcatag cagttttaac tctatttact
     4621 cctcttaaag attatttaaa ctcgaaaact aaaattgctg attactactt ggtttttagt
     4681 tctttaga
//
LOCUS       DAVKWS010000092         4445 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKWS010000092 DAVKWS010000000
VERSION     DAVKWS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.093"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            106..483
                     /locus_tag="VW852_001994"
                     /pseudo
     CDS             106..483
                     /locus_tag="VW852_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
     gene            477..599
                     /locus_tag="VW852_001995"
                     /pseudo
     CDS             477..599
                     /locus_tag="VW852_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            603..1736
                     /locus_tag="VW852_001996"
     CDS             603..1736
                     /locus_tag="VW852_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0273070.1"
                     /translation="MAIRKGKDGNWVVDISNGFDPITLKQRRLVRKGYKTKKEAIEAE
                     HYLRSVELKERFYGAKITISILYELLKEEDSINHRKASYINTQDNNYNKHIKDYFSMV
                     DNVGKLTYEDIYQFREHLRQKVAKNSDKPLSTNTINKIMILLKKIFDVGLRKGYYSSN
                     PAKLIKKLPIEKTKMQFWTVKEFQKFLTLFEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFSSNTIHITKSVYVNKGVTHISTTKTKAGTRRIVINKKLSQELQDWEKRQKHL
                     LEQFTNNSLTLQVFQNSPITITKNAIEKQYKKIIERDSTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            1777..2214
                     /locus_tag="VW852_001997"
     CDS             1777..2214
                     /locus_tag="VW852_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV0273071.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPETVASFVATDEE
                     KAVAYLHDVIEDTSLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKKNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRAKKKS"
     gene            2430..2666
                     /locus_tag="VW852_001998"
     CDS             2430..2666
                     /locus_tag="VW852_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEV0273072.1"
                     /translation="MNNVVTTQISIQLSQELNSLLNAIVEENNTTKTDFIRQAVIEKI
                     EDMYDIKVADEAYKKWVECGKKAISHEEMMRRYG"
     gene            2659..3390
                     /locus_tag="VW852_001999"
     CDS             2659..3390
                     /locus_tag="VW852_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0273073.1"
                     /translation="MAKLYAISDIHGYLDEFRDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETDTQIFVHAGIDEEA
                     GKLWKELTSSEIFTNKFPITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYFI
                     DGDVTNSKTIPILCYDTITQKYHFL"
     gene            complement(3501..3584)
                     /locus_tag="VW852_002000"
     tRNA            complement(3501..3584)
                     /locus_tag="VW852_002000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3548..3550),aa:Leu,seq:caa)
     gene            complement(3594..3665)
                     /locus_tag="VW852_002001"
     tRNA            complement(3594..3665)
                     /locus_tag="VW852_002001"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Gln,seq:ttg)
     gene            complement(3676..3748)
                     /locus_tag="VW852_002002"
     tRNA            complement(3676..3748)
                     /locus_tag="VW852_002002"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3713..3715),aa:His,seq:gtg)
     gene            complement(3763..3833)
                     /locus_tag="VW852_002003"
     tRNA            complement(3763..3833)
                     /locus_tag="VW852_002003"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3799..3801),aa:Trp,seq:cca)
     gene            complement(3838..3918)
                     /locus_tag="VW852_002004"
     tRNA            complement(3838..3918)
                     /locus_tag="VW852_002004"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3882..3884),aa:Tyr,seq:gta)
     gene            complement(3931..4003)
                     /locus_tag="VW852_002005"
     tRNA            complement(3931..4003)
                     /locus_tag="VW852_002005"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3968..3970),aa:Phe,seq:gaa)
     gene            complement(4007..4080)
                     /locus_tag="VW852_002006"
     tRNA            complement(4007..4080)
                     /locus_tag="VW852_002006"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4044..4046),aa:Met,seq:cat)
     gene            complement(4091..4180)
                     /locus_tag="VW852_002007"
     tRNA            complement(4091..4180)
                     /locus_tag="VW852_002007"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4142..4144),aa:Ser,seq:tga)
     gene            complement(4263..4336)
                     /locus_tag="VW852_002008"
     tRNA            complement(4263..4336)
                     /locus_tag="VW852_002008"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4300..4302),aa:Ile,seq:gat)
     gene            complement(4371..4441)
                     /locus_tag="VW852_002009"
     tRNA            complement(4371..4441)
                     /locus_tag="VW852_002009"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4407..4409),aa:Gly,seq:tcc)
BASE COUNT         1638 a          803 c          795 g         1209 t
ORIGIN      
        1 tcacaaatgg gaaaagctac gtgactttcc cttggttgcg tgaaatagct tgcagaaatt
       61 ataaaggaga aactaaggtg tttactacaa aggaactcta taatttccta tattaaacgc
      121 tttaactatc acatttttaa taccaagaaa agtaaattaa aatatatagc agtattagag
      181 aggcaaaaac gtaaagcatg gcatagtcat attttgctat tcagtgttcc ctatattcca
      241 cataagcaat tattagaact ttgggggcat ggagcaaata aagttgacgt tgacagcaaa
      301 gaaaatcgcg gtcgatatgt cacaaagtat tttgaaaaag gtattggaca agaattgttg
      361 gaaaacttcg gaaagcaagc ctatttttca tcacgcaact tgaaaaagcc tgatgaagat
      421 aaattttaca cttatgaaga ttttaactat gatagttcag tcgtactata tgaaacggaa
      481 tacaactgat aacccgaggc tttacagtct atcagaacaa gcgaattggt actatacctg
      541 ctactattgc taccgaatta ctcggttttg atgttcaaaa cagtaccctc aaggagtaaa
      601 atatggcaat tagaaaaggt aaagacggta attgggtagt tgatattagc aacggttttg
      661 acccaataac actaaagcaa cgtagacttg tcagaaaggg ctataagacg aaaaaagaag
      721 ctattgaagc agaacactac ttacgaagtg tagagttaaa agaacgcttc tatggggcta
      781 aaataactat ctccatacta tatgaactac ttaaggaaga agatagcata aatcatcgaa
      841 aagcaagcta tatcaataca caagataata attacaacaa acacatcaaa gattattttt
      901 ctatggtcga taatgtcgga aaactaacct atgaagacat ctaccaattt agagaacatc
      961 ttaggcaaaa agtagctaaa aattctgata aaccgttaag cacaaatact attaacaaaa
     1021 tcatgatttt actcaaaaaa atttttgatg ttggtttgcg aaaaggctat tactcctcca
     1081 atccagcaaa attgataaaa aagttgccaa ttgaaaaaac aaaaatgcaa ttttggacag
     1141 tcaaagaatt tcagaagttt ttgacactat ttgaacccga agaatacaat atcaaactac
     1201 tctttactgt tctattcttt acaggattaa gacttgggga agctcttgcc ctaacatggc
     1261 aagacatcga cttttcaagt aatacaattc atatcacgaa atctgtctat gttaataaag
     1321 gagtaactca tatcagcaca acaaaaacta aagcaggaac aagaagaatt gttatcaata
     1381 aaaaattaag ccaagaatta caagattggg aaaagcgaca aaaacatcta ttagagcaat
     1441 ttaccaacaa tagcctaaca ttacaagtgt ttcaaaatag tcctatcaca attacgaaaa
     1501 atgctattga aaagcagtac aagaaaatca ttgaacgtga tagcacacta aaaaagatac
     1561 gcatacacga ttttaggcac tcacacgctt ccctgctgat taaccaaggg gaagattatc
     1621 tagttgtaaa agagcgacta ggacatgctt ctataacaac cacgattgat acctactctc
     1681 acttgtaccc aagtaagcaa aaggatttag ctgataaact agatgattta ctataaagta
     1741 attgagtttt ataaatggaa agtttataat taaatcatgg atacatcaca agtaataaaa
     1801 gcacatgaag tagctaaaaa agcacacttt ggacaaattg atagagcagg aattgactac
     1861 attaaacacc ctgaaacagt agctagtttt gtcgcaactg atgaagagaa agctgtcgcc
     1921 tatctacatg atgtaattga agatacttca ctaaccctac tagacctaaa aaaggaaggt
     1981 ttttcaaaaa acattataga agccgtcgat atactcacaa agaaaaaagg acaagattat
     2041 caatcttatc tcaatctagt aaagaaaaat gaactcgcta gagttgtaaa gttggcggat
     2101 ttacggcata attcagattt aacaagacta ccattaatta ctgaaaaaga cttagagaga
     2161 aataagaaat acagttctgc tattagattt ttaagagcaa agaaaaaaag ttagtaacta
     2221 aactataaaa ttttataaag agaaaacaaa agacaaggct tataaacttt gtctttatta
     2281 ctatactggc ggtcggtctc gaactggcac atccttgcag atactacaat tttattaaaa
     2341 taaataacta taaaaagtga aattttttat tgagaaactt ttagactagg agtataatgt
     2401 atctaaaata actattaggt ggctgttata tgaataatgt tgttactaca caaatttcta
     2461 ttcaacttag tcaagagtta aattctttat taaatgctat tgttgaagaa aataatacaa
     2521 caaagactga ctttattaga caagctgtta tagagaaaat tgaagatatg tatgatatta
     2581 aggtcgctga tgaagcttat aaaaaatggg ttgaatgtgg caaaaaagct atctcacatg
     2641 aagaaatgat gagacgctat ggctaaatta tacgcaatat ctgatataca tggatacttg
     2701 gatgaattca gagacgctct caataaagta aatttaaacg ataaagataa tcgcctattt
     2761 ttactaggag attatcttga taatggactt caatcttttc aagtcatttc caaaataatc
     2821 gaacttgagg aagtttatcc taatcaaatt attactcttc tagggaatca tgaagaatgg
     2881 ttttatgatt ggttgatttt agataagcct actgcttcag catttccaga aacaataaaa
     2941 agcttcttct ctccagagga attgaactat atttttaaat cgaatgctaa caactttgaa
     3001 acaggtgtta gaaatgaaat taaaaataat atcaaattca atccatttat aaactggttt
     3061 aaaaaacgat atagggataa acgctattat gaaactgata cccaaatttt tgttcatgct
     3121 ggcattgatg aagaagccgg aaagctttgg aaagaactaa cgagttcaga gatatttaca
     3181 aacaaatttc ctattaccac tggtagattt cataaagcta ttatttcggg ccatattgct
     3241 tcttgggaag tagctaaaga tagaagatac ctgggtaaga tttactatga tagtaaaagt
     3301 cattatttta ttgatggaga tgtaacgaat agcaaaacaa tacctattct ttgctatgat
     3361 acaattaccc aaaaatatca ctttctataa cttttaatca tgtgctttta tatgaaatta
     3421 gtaattatta gtaacaattc caagaaattt cttatgacat aacaaaaaga caaggcttag
     3481 aaaccttgtc ttttcaacta taccggcggc cggggtcgaa ccggcacgtc cttgcggaca
     3541 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaaatag taactggggt
     3601 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
     3661 ccccaatata aaataggcga gtgatgggga tcgaacccac gcatgccaga gccacaatct
     3721 ggtgtgttaa ccacttcacc acacccgcca taattctatt aacacgggca gtaggaattg
     3781 aacccacact gaaggttttg gagaccttag ttctaccttt aaactatgcc cgtaaaatgg
     3841 aaggggaggg attcgaaccc ccgaacccga aggagcggat ttacagtccg ccgcgtttag
     3901 cctcttcgct acccttccaa aatatataaa tggcgcgaga cggaatcgaa ccgccgacac
     3961 atggagcttc aatccattgc tctaccaact gagctaccga gccttattgc gggagcagga
     4021 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccgagctgct ccatcccgcg
     4081 ttaatataaa aggaggatgt gggattcgaa cccacgcacg cttttacacg cctgacggtt
     4141 ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc aaaatagttt atacgggaac
     4201 aagcccgtgt cctcttagtg aacagataat attccaatcc cctagtaatg gactaaacac
     4261 tatgggcacg agtggactcg aaccaccgac ctcacgctta tcaggcgtgc gctctaacca
     4321 cctgagctac gcgcccaagt caaaaacttg gtacaaagaa caaagttcaa agcgggtgac
     4381 gagaatcgaa ctcgcgacaa cagcttggaa ggctgtagtt ttaccactaa actacacccg
     4441 ctaaa
//
LOCUS       DAVKWS010000093         4269 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKWS010000093 DAVKWS010000000
VERSION     DAVKWS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.094"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(437..634)
                     /locus_tag="VW852_002010"
     CDS             complement(437..634)
                     /locus_tag="VW852_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0273074.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(619..897)
                     /locus_tag="VW852_002011"
     CDS             complement(619..897)
                     /locus_tag="VW852_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV0273075.1"
                     /translation="MFVIEEVKDENQKKTVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(936..1721)
                     /locus_tag="VW852_002012"
     CDS             complement(936..1721)
                     /locus_tag="VW852_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0273076.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1723..2541)
                     /locus_tag="VW852_002013"
     CDS             complement(1723..2541)
                     /locus_tag="VW852_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV0273077.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2543..3535)
                     /locus_tag="VW852_002014"
     CDS             complement(2543..3535)
                     /locus_tag="VW852_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0273078.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1428 a          916 c          736 g         1189 t
ORIGIN      
        1 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg
       61 taatcagtaa ccatacgcac ggcaaggcga cgttgacgtg gtttgaagag attttctaag
      121 agtataagtc caaaactttc gttaaccggg atacattata agcacattca taccatcaaa
      181 aatagtagca ataaacttga acaactacca gaaacacgaa aacaggcata attgatgaaa
      241 tatcagcgct attaaacaat ataaataacc gctaagaata aggcttaacg gttatcttct
      301 acctatctat tttttcaact cctcagtcat ttatctacct ataacctagg ctttacgagt
      361 gccttgctct accaactaag ctaaatcggc gattacactt acagtatggc actctgagaa
      421 tagatgtcaa gtaatattat tcaagctttt taatcaaaat ctgacaagga ttttgcggat
      481 tccatagctg agaaaaaatc tctaactttt taaagccaag acctcgataa aagtcatttg
      541 ttcgatcata atctttatta gaaccttctg ccactgtttt gacctgcaga taaccaactt
      601 ttttacgagc ttcactctct aaagtagcaa gcaattggct cccaattttt ctaccttgat
      661 aagctttttt acgccgagac aatcaatctc tgcacaatct tcactcgaat aggataagct
      721 tacaaatcca gtcaaatcac tctcctgata ggcggtccaa acttgcagtg tcgtggttcc
      781 ttctatatag gcttgtgtgc tttctgggat tccaaaccat tctggcaaat ccttcaaaac
      841 ctcagctaca actgtctttt tttgattttc atccttgact tcttcaatta caaacatcat
      901 aagtttcttt cttgatttta gtcttacttt agcttttacg aacccgcact ttcgtaggaa
      961 tctaagccct tatcccaaag tttaagggaa ataacaaaga aaaccagaga aatcaacatc
     1021 aaacctccta cgttaaagaa cacatccttt tcctgtaaga aatagctagc tggatagtag
     1081 gctgtaaagg cgaaaggcac gataaagcta atcaaccaac gaagaagaga attgtaaata
     1141 gaaatcggat acttagcaaa gtcattgaac atatagaaga tgtaaatcat ggcgcctgac
     1201 tgcttagtcc aaaaggcgat actggctgtt gcgattttaa gagaagtata aatcaaggtc
     1261 gcaaaaggaa tacaaactag gaaaagcagg aattttggaa gagtccaaac aatgctggtc
     1321 actgttgttc ccaataaaat accaccgact aagagttcac ccaaggcatc aatctgaaag
     1381 gtttcaacta ggatgtgaaa gagaggattg atgggacgag tcagatactt gtcaaactct
     1441 ccttttcgga ctaggcgttg ccctagtgcc cagagattgt caaaaaagag atggtccatt
     1501 cccttgggaa tcaaggaaaa tccataaatg aaagctatct cttgaaaggt ccagccttct
     1561 aggaatggaa tatgttgaaa gatgacattg agaaacaaga gattcaagcc ttgagccaga
     1621 aagactccca agacaccaac cacaaaatcc accttatatt ccatgatttg tttgatgtat
     1681 tgtctgataa aaatcagatg cattcgttga tattttttca tactaacctc cttgaatggt
     1741 gataaaggac tggacccgtt tccaaattaa ctgagacaat cccaccatca ctaagagcca
     1801 gaagaactgc aaaaggagtg cctgaagaat ctgactggca tcgtattttc caacaatgat
     1861 cataactgga gtataaatca aggatgaaaa aggcaaaaag gagagaatat ctgaaacaac
     1921 ctttggaaag aatgccaagg gaatcaaact ccccgacata aaagccacta tggaagtctt
     1981 aagtaggttg gaaccccaaa gatttttaaa cacaaaggct gaaaatccaa agcaaatatt
     2041 aaagaaaaag ttaatcagat aggcgagcgt taagctaaaa agataaatga cagttaatcc
     2101 tagcacctct acaatacctt gacccgatat gattttcatc aagacaatga cacttaaaaa
     2161 tggaaggcca acgctgataa aaatcaacca cttggaacca agctcggtga aaagatagga
     2221 ggccgcaaaa tgcactggtc gcaacaaacg catgataatg gagccatcct tgacctcctc
     2281 cccaatcata aaggacgaat cggatctagt cagaagattg gtcacaaaac tcatgatgat
     2341 gtagagggtg atatccgcca tactaaagcc ctgaatcaaa gactcttgcg aagaatcaaa
     2401 gacagccttc cagagataaa aggccacaaa agcccccatg acatcgccaa tccgatagag
     2461 aataaagttg actcggtaag taatcaactc ctgaacccct gcattgataa agggtttata
     2521 acgtctccac aatttgatca tcctagagct cctttcggta gaagcgacgg ataatatcct
     2581 caatatccgt atccaccatc ttcaaatcgc ggatttcaaa atcagacagg gtttgcttga
     2641 taatgtcagc tgactggtag cgagaactat caaattcaat gttgaggctg tttccttgtc
     2701 tatcaatggt catatcagac agaccgtcat agtgagagac gagatgactt tgacctggta
     2761 gcagttcaaa agagagagtc ttcatcttac caaaggtctc cttgagttgg ctcaccgttc
     2821 catcaaaaat ctcttgcccc ttgtcaatca tgaaaatccg atcacaaagt tgctcaatat
     2881 cactcaaatc gtgagtggtc aaaagaatgg tagtttcttc ctcttgattg atctgagtaa
     2941 ttgcccgacg aatattatcc ttaaccgaaa cgtccaaacc aatggtcggc tcatctaaaa
     3001 aaagaacctt gggattgtgg agcaaggagg ccgcaatatc cgcccgcatc cgttgtccca
     3061 gtgaaagagt ccgcacggga tccttgataa agtccttcaa atccaagact tcattcaaaa
     3121 agtccatacg cttatgaaag agcgagtctg gcacatcata aatctctttt aagacagtgt
     3181 aggtctcttg cagagccaaa tcccaccata gctgggtgcg ttgtccaaag actacgccaa
     3241 tatctttgac ataatcttgc cgattgttct ggggaatctt gccgttaatc cgacaaaaac
     3301 cagatgttgg tttcaaaatt cctgtcagca ttttaatggt tgtcgacttc ccagcaccat
     3361 ttgccccgat aaatcctaaa atctgccctt ttggaacttc aaaggtcaaa tccttgaccg
     3421 cttcaaaggt ctgcttttca ggatgaataa aggagcgcaa agcccccttc aagcccggtt
     3481 ccttaacagt cttcacaaaa tttttctgaa gatgttccac ttctatcatt gccatatcta
     3541 tctccctatc ataaggaata gcaacattgt gtttttaact acaaatattc taaatcctta
     3601 ctttctacac cttctacatt ttattactac agaagacttt ataccaacct attatagtcc
     3661 aataaaaaac agaatgcaag cgtttgccta tggattgtga aattaggatt tctctcatga
     3721 aataatactt ttaatcaaaa attctgttta atagtttaaa ttaccaacct actcatcttc
     3781 tcaccagagg gcggtattaa ctttgatcat aaaatcggat actccctcta tccagaaaat
     3841 atgctcagaa tgattgtctc taaacaatac ctgctctttt acctcattca tgaaaaagaa
     3901 gtccataccc tgagaattat caactcacgc accgactacc taaaccaact cgaccacctc
     3961 tttcgtacat gtaggaaact attttcctca caaataatct cattataaaa tccaataaat
     4021 tttttagctc acttgaatat tttctttgta tcgttttatc agcttcaatc tgatctagac
     4081 ttgttacctt ggaacgactt gaaataacct ttataaaata acacttaagg ctttcatcat
     4141 ctcgtataaa tttttcaata gtgatataaa aagctttcat attctgttta taatcagata
     4201 ttaattgatt aattttatca ttctgatgaa gattcgatac cataatatat agcggattga
     4261 aataagatg
//
LOCUS       DAVKWS010000094         4165 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKWS010000094 DAVKWS010000000
VERSION     DAVKWS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.095"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            49..524
                     /locus_tag="VW852_002015"
                     /pseudo
     CDS             49..524
                     /locus_tag="VW852_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            592..1044
                     /locus_tag="VW852_002016"
     CDS             592..1044
                     /locus_tag="VW852_002016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273079.1"
                     /translation="MFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCEGISGIILY
                     LCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVI
                     LACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            1055..2287
                     /locus_tag="VW852_002017"
     CDS             1055..2287
                     /locus_tag="VW852_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0273080.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLAMLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(2405..3424)
                     /gene="cbpC"
                     /locus_tag="VW852_002018"
     CDS             complement(2405..3424)
                     /gene="cbpC"
                     /locus_tag="VW852_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV0273081.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPTTGIM
                     QTGWQYLGNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNASNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(3522..>4165)
                     /locus_tag="VW852_002019"
     CDS             complement(3522..>4165)
                     /locus_tag="VW852_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV0273082.1"
                     /translation="FLSLELQKEQYRVDLVEEGQKALSMALQTDYDLILLNVNLGDMM
                     AQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIYKPVFIENLVARISAIF
                     RGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTRREYDLLATLMGSKKVL
                     TREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIKTVRGVGYTMQE"
BASE COUNT         1235 a          915 c          693 g         1322 t
ORIGIN      
        1 agtgttctac gatttttact agataaaact gtaaattctg aaggaaggat cacttcttca
       61 acagaatttg gaaatttcgt aagtaattta tcattccaac acggaatagc tggactactg
      121 tttcctctaa ataaattgta ccccccagaa ctggattcta aaatactctc tatcatcaag
      181 aaggcagtga caattagaac gacacacaca tataaatatc aatactcact gctatttggt
      241 gatgcaggct atctatggtt actcctacat ttattttcta tcagtaaaaa tcaatactat
      301 ctacaattag caaacgtctc cgctaagaaa ttaatagaga attatgatac tctagaggaa
      361 atagactttg cattgggaaa atctggtgtc ctattatcat taataaaata ctatcaattt
      421 accaatgaca atactcttaa aattttcatc cacaatagta taggggaaat ttagcattat
      481 ttcctacaaa gagatatagc caaagaaagc attttagact atagctttgc tcatggatat
      541 tgtggaattg cataggcttt atttgcctat tctaaagtct tagaaccttc tatgttctat
      601 aatgatctcc atacattcca tactgaatta aaaaaattat tagaaaaagt tacttctaat
      661 actgaaaatt taggaaattt acaactttct tggtgcgaag gaatttccgg aataatctta
      721 tatctttgta tgtacgattg tgacggaaac aaagatatta ttagtaaata tcaagaattt
      781 gtttttaacc atcatctaaa aatgatgaca ggatattgcc acggaataac tagcttacta
      841 caaaccactg tctacaatca aaacaaatta ctgatgaaaa aaatccaaca ggtaatttta
      901 gcatgttctg aacgagatga tcacggttta ctgatgtttc aaggagatag tggtaaagca
      961 gatttgtttg acttcggaat aggaagcatg ggggtatatt ggtgtctatt aaataataaa
     1021 ttcccattcg atgtgcagac ataaggagaa aagtatgaaa ttattttgga caaacaacat
     1081 atatagacag ttgctgctaa acagctgttt ttcatcattc ggcgacagta ttttctacct
     1141 cgccattatc aattatgtgg ctcagtacaa tttcgctccg ctagcaattt tactgatttc
     1201 catttcagag atggttcccc tactatcgca actctttctc gggattctag gagattttca
     1261 agaaaataga gtcaaacacg cactctggat tgccaaaatc aaaatcctgc tctacgctat
     1321 tttgacagta tttctcgtct tgtcgccctt ttcattagtt tcagtcatta tgattgtcat
     1381 catcaacctc atctctgaca ccttgagcta cctgtctgcc tacatgatga acgccctcta
     1441 catcagtgta attaaggacg acctgcatga tgccatgggg ttcaggcagt ctctgatgag
     1501 ggttgtccgc attgtcgcca atctggctgg cgcattcctt atcaatgtta tgagtattca
     1561 aactatttcc cttatcaaca ctctgacttt tgtcattgcc tttttgggcc tgtatgttat
     1621 tcgacatacc ttgtatgagg ttgaaaaaag gattgaaatg tcacatacag cactgagttt
     1681 taagaaatat tttcaacatc ttaaacagtc gctggctgtg ctcctgaggt taaaagatac
     1741 cgtcatacta ctgtttctga cgaccagtat gattgccatc ttggatgtgt cccctcggct
     1801 gattgccctc cgcttcatcc aacagacact agcacaactg agcattgggc aactcctcgc
     1861 catgctctcc atcatcattt cttgtggagc tatccttggc aatatgacca gcagtaatct
     1921 atttaaaaat atccgtttca cgcacctctt ggttttctgt gagatttccc tattggctct
     1981 aataactagt atcctttgtc aagcctatat cgtaattttc atgaccagtt tcatcagttc
     2041 tacgattatc ggcattctca gccctcgcct acaagcagct gtctttgccc atatccccag
     2101 tgacaagatg gggacggtgg gctctgctct gagcacagta gacattctcg ccccgtccct
     2161 gctctcccta ttagccctgt ccatagcatc gggcgtttcg gtgcagttag cattgatatt
     2221 tttgtatctt attttgattg ctcttatctt ttgtcaatgg ttagtcaagt tcaacactca
     2281 taactaacga aaaagcatgt gtagatttca catgctttta atctccccaa tcgtcaggtc
     2341 aagtacaaca aagtcacttc tttgattaag cgagtgttct aatataatta taagcgccct
     2401 gtcattaccg aacccattcg ccattataat tgacagaata gccatctacg gtcgtattca
     2461 ctgccaaagc acctgagcta taagcatagt accagttgcc attgacctgg aaccaacctg
     2521 tcttcatatc tccattactt gcatttaggt agtaccaagt tgaaccatct tggtaccaac
     2581 cagttgccat agctcctgat gaacggagat agtaccattt gttcccaagg ttttgccaac
     2641 ctgttttcat atcgccattt ggctggtcta aataatacca agtggtacct tcctgatacc
     2701 agccagtggc cattgctcct gaggaacgga ggtagtacca cttattacct agatattgcc
     2761 aacctgtttg cataatacca gttgttggat ctaggtagta ccatggagca ggtttgcttc
     2821 tatcaatgtc aatagtaaca tgaaaatctt gagtccaacc cttagcaact tcaccaattg
     2881 gtagtggatt gtaagaatca tcaccaaaat ttcctagctt atttaaataa taccaattgc
     2941 cgtcttcata aatccagcct gtctttacag cataattatt atcaaaataa taagttctct
     3001 tttcagcaga tagaggatat tgttcaccgt atacttttcc tgtattttca gacgtttttg
     3061 cctctagtac ttgtttatct gtttgttcta gcaaagcacc atcttgtcca aaatagtacc
     3121 acttctcctg aaggccaatt tcgaagactg gacgattatc aaataaatca tcacgataac
     3181 ctgttccagg aatttctaag tattgccaac cgacaaccat ctctccaata tcaccaaaat
     3241 agtaagtttt accgtctatt ttatgccaat agattgcttt atgatcatct tttacataat
     3301 attttctatt atccttatca acaaatcggc cacctgtggt attcgcaaat accgtatttg
     3361 tagctagcaa accaaagaaa aagactgcta gtagaacttg catcattttt ttcaaaagtt
     3421 tcatctatat atcctcctaa aacgattgta gcaaagaccc gactgcatgt caatatataa
     3481 aaactcctca aaaaagcagt tacacaactg caactgcttt tctattcttg catggtgtaa
     3541 ccaacaccac gcacggtttt aatgtagctt ttttgacctt taacatcaag cttgctccgt
     3601 agatagcgga tatagacatc tacgatattt gtctcggtcg cactttcata cttccaaaca
     3661 ctttccaaca gttgctcacg agtcaatact ttcttgcttc ccataagtgt cgccaaaagg
     3721 tcatactcac ggcgggtcag agcaatcatc tcttcaccac gataaaccgt gtgatgttca
     3781 acatctatcc taagattgcg gtaggtcctt ggaactttca tcagactgca gtgttgatca
     3841 atgaagtccc gacctcggaa gatcgccgaa atacgcgcta ccagattttc gataaagact
     3901 ggcttataga tgtatgaaac tgcaaaacgc tgaacaactt ccagctcttc ttgcaagtct
     3961 tcccaatgat ctaaaatcat gatgactgag gcaggtttag ttcgactcaa tttttctgca
     4021 aaatcctgag ccatcatatc tcccagatta acgttcaata aaatcaaatc atagtctgtc
     4081 tgaagagcca tggaaagggc tttttgcccc tcctctacca gatcaacccg atactgctct
     4141 ttctggagtt ccaaacttaa aaaat
//
LOCUS       DAVKWS010000095         3668 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKWS010000095 DAVKWS010000000
VERSION     DAVKWS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.096"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            479..817
                     /locus_tag="VW852_002020"
     CDS             479..817
                     /locus_tag="VW852_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV0273083.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKIKGTRITK"
     gene            843..982
                     /locus_tag="VW852_002021"
                     /pseudo
     CDS             843..982
                     /locus_tag="VW852_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            2033..2272
                     /locus_tag="VW852_002022"
     CDS             2033..2272
                     /locus_tag="VW852_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273084.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            2257..2535
                     /locus_tag="VW852_002023"
     CDS             2257..2535
                     /locus_tag="VW852_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273085.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKITSCCIVAKDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            2821..>3668
                     /locus_tag="VW852_002024"
     CDS             2821..>3668
                     /locus_tag="VW852_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273086.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEVKKKAEDAQKKYDEGQKKTVEKAKREKEASEKIAEATKEV
                     QQAYLAYQQASNESQRKEADKKIKEATQRKDEAEAAFATIRTTIVVPEPSELAETKKK
                     AEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLEQEVATAQHQVD
                     NLKKLLAGADPDDGTEVIEAKLNKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQD
                     ELDKEA"
BASE COUNT         1321 a          468 c          742 g         1137 t
ORIGIN      
        1 tacttgacag ttattatatt ttcgcttatg cttattatat agataaaagg acgggatagg
       61 agtttaaaaa tttgaaagcg ctatccataa tcataacgca taattttctc cttttcgtga
      121 aaaaagttaa cgtaattttg aaatgagctt aataagaggt actatgtagg tagaaaataa
      181 agataggaga aaaagtatga ataaaaaaat attttgtatt ctagtatgta tgatatcatt
      241 gattagtctg gtcataatct ttccatgggg ctggtcgata taatgtaagg gagcttgagt
      301 tgttaaagga ggctcagtcc ttggacaaaa atctgctgta ctacaatgct tggaaagata
      361 tcaagaacaa agaatgatac gattgaaaat aaactgattc aagaactact gggcagtgat
      421 tgaggtttgg gtgacatcaa agaaaagtta gattaattct aaagaattga gtatttctat
      481 gttaaaaaaa tatccttgta cgatgcaaca tgatcagtca gattgtgctg cggcagttgt
      541 ttcaacagtt cttttatctt acaaaaagga actatcaatc atgaagattc gggaaattat
      601 tggtacagac atgtatggaa cgactgtcag tggtattgtt tcaggtctga ataagttgaa
      661 ttttacagta aaagctgttc gagtggcact ggaagatttg actccaaaat taacatttcc
      721 tgcgattctt caagttaaga atgatttagg tcaaaatcat tttgtggtat tacatagtat
      781 aaaggagaaa ataaagggaa ctcgtattac caaataatta tgttgctctt gagcaagagg
      841 agatgatgta tcttgatggg ggggcttatt taagcaagag tgcttgtcaa ggaatttgcg
      901 cagctttagc tatgagccca ggaactttta tagcattgac tggagctgca gttttaataa
      961 aaaaactaat aaactatatt aaggttggag gccttggagg ttggcttatt ggtgcagtag
     1021 caggtgtatt ggctcttatc tttttgataa aaatttgtgt agataccacg ataaaatttt
     1081 tattcatcag attatttgac acaagtactt taatgaagag gagaatattt tttcttttcg
     1141 cattggatgt tatagcttac ttatttttag gatatctttt agtgattcaa aaagcaggct
     1201 atttgttttc ggttttactt cttttttcta atttttcagt tccatttatc aaagaaaagg
     1261 aatacgaatt atttaaaaat agcaagtaat agaaatatgg attaaatctt agaacggctt
     1321 aaaatcagat atgataaagg gaaatagtct tatctaggta actaagtgct acaaacacaa
     1381 tcgaggatca cttgttagga ttacatttta atcatgctta ataagaatgg agaattccta
     1441 tggggattgt attactctga agtttcgaat accttagttc tcgtgatttt gagctattct
     1501 agctttagaa tccttcagaa cgtaaaattt taagcagtca aagacttaga atagcccgaa
     1561 aacatttatt ttatagcaat tctaggctta gaattgctca tatatatgta tttgagctgc
     1621 tcgatagtta ggacagctct attctgagaa atctgaccta tcaatgcttc agaatagtat
     1681 ttcgccaatg gtttttattg aagaggtgat tgttttatga tagtctaaaa taaaactgat
     1741 ttaatggagt aaatggagaa tcgttacatg aaacattttt ttgcaggaat tggtgagata
     1801 aaacttgttg gctacttatt gtatatatgt gtaggactag cccctctttt tcatatatat
     1861 gtcataggtt cggaactgag ttttgtgaaa atagtgttat ctattttggg agttatattt
     1921 gtaagtatgt taactattgc tcgaatttat cggacttttc tataaagaat agtttggact
     1981 tgaactgtat ctaaaaacag aggttggtcg attatcagcc tctttcttgt cgttgaaatt
     2041 acttgattgt attttggact atcaagaaag attcaatgga aaaacgtgtc aagtatcaac
     2101 gaattataag tatttagaga ttttcaaagt gaatttttgc ttgactgatt tacatcattt
     2161 atttggctta cacaaaatca cacgagatta tgctagtcaa acaaaacctg ctattcaagc
     2221 tggtgttttt attttagaag attttagaaa tatcctatgt acaatgatgt aatcgaaagg
     2281 atatctttat atgagtttat aggtgatatt ttctattcta aaataacaag ttgttgcatc
     2341 gtagctaagg atttatctaa aaatactatg aaattggacg tcatattttt tgaggataaa
     2401 aataaaagat ccgcagtttt aggtttacga agagacaaaa gcggagtatt taaaccagtt
     2461 actctacatt ttacaagcgc taagaaatat gctaaagttc gtaaaacaga tgtgaaagaa
     2521 atgaaatggc tataaagatt gtccgcaggc ttaagcttgc gttttttgtt gtttaacgaa
     2581 attaaaaaat aagctctctc attggaaatg tttttatagg aatgaaggaa gatgatgcta
     2641 tgtttaaaaa gagaatcgaa aagggcaaaa gtagtgtctt tatctttttg gagtagaagc
     2701 ttatgatata gaaatttgta ataaaaatgt aatataaaac atttgacaaa tatttacggg
     2761 gggagtatac ttaatataag tatagtctaa aaatgactat cagaaaagag gtaaatttag
     2821 atgaataaga aaaaaatgat tttaacaagt ctagccagcg tcgctatctt aggggctggt
     2881 tttgttacgt ctcagcctac ttttgtaaga gcagaagaag ctcccgtagc tagtcagtct
     2941 aaagctgaga aagactatga tgcagcagtg aaaaaatctg aagctgctaa gaaacattat
     3001 gaagaagtta aaaagaaagc agaagacgct cagaagaaat atgacgaggg tcagaagaaa
     3061 acggtagaaa aagcaaagcg agaaaaagaa gcttctgaaa agatagctga ggcaacaaaa
     3121 gaagttcaac aagcgtacct agcttatcaa caagctagca acgaaagtca gagaaaagag
     3181 gcagataaga agataaaaga agctacgcaa cgcaaagatg aggcggaagc tgcatttgct
     3241 actattcgaa caacaattgt agttcctgaa ccaagtgagt tagctgagac taagaaaaaa
     3301 gcagaagaag ctaaagcaga agaaaaagta gctaagagaa aatatgatta tgcaactcta
     3361 aaggtagcac tagcgaagaa agaagtagag gctaaggaac ttgaaattga aaaacttcaa
     3421 tatgaaattt ctactttgga acaagaagtt gctactgctc aacatcaagt agataatttg
     3481 aaaaaacttc ttgctggtgc ggatcctgat gatggcacag aagttataga agctaaatta
     3541 aacaaaggag aagctgagct aaacgctaaa caagctgagt tagcaaaaaa acaaacagaa
     3601 cttgaaaaac ttcttgacag ccttgatcct gaaggtaaga ctcaggatga attagataaa
     3661 gaagcaga
//
LOCUS       DAVKWS010000096         3663 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKWS010000096 DAVKWS010000000
VERSION     DAVKWS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.097"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..1447
                     /locus_tag="VW852_002025"
     rRNA            <1..1447
                     /locus_tag="VW852_002025"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1499..1571
                     /locus_tag="VW852_002026"
     tRNA            1499..1571
                     /locus_tag="VW852_002026"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1532..1534,aa:Ala,seq:tgc)
     gene            1696..>3663
                     /locus_tag="VW852_002027"
     rRNA            1696..>3663
                     /locus_tag="VW852_002027"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1030 a          748 c         1088 g          797 t
ORIGIN      
        1 tgcgaacggg tgagtaacgc gtaggtaacc tgcctggtag cgggggataa ctattggaaa
       61 cgatagctaa taccgcataa gagtggatgt tgcatgacat ttgcttaaaa ggtgcacttg
      121 catcactacc agatggacct gcgttgtatt agctagttgg tggggtaacg gctcaccaag
      181 gcgacgatac atagccgacc tgagagggtg atcggccaca ctgggactga gacacggccc
      241 agactcctac gggaggcagc agtagggaat cttcggcaat ggacggaagt ctgaccgagc
      301 aacgccgcgt gagtgaagaa ggttttcgga tcgtaaagct ctgttgtaag agaagaacga
      361 gtgtgagagt ggaaagttca cactgtgacg gtatcttacc agaaagggac ggctaactac
      421 gtgccagcag ccgcggtaat acgtaggtcc cgagcgttgt ccggatttat tgggcgtaaa
      481 gcgagcgcag gcggttagat aagtctgaag ttaaaggctg tggcttaacc atagtaggct
      541 ttggaaactg tttaacttga gtgcaagagg ggagagtgga attccatgtg tagcggtgaa
      601 atgcgtagat atatggagga acaccggtgg cgaaagcggc tctctggctt gtaactgacg
      661 ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgctgtaa
      721 acgatgagtg ctaggtgtta gaccctttcc ggggtttagt gccgtagcta acgcattaag
      781 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc
      841 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga
      901 catccctctg accgctctag agatagagtt ttccttcggg acagaggtga caggtggtgc
      961 atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc
     1021 ctattgttag ttgccatcat ttagttgggc actctagcga gactgccggt aataaaccgg
     1081 aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta cacacgtgct
     1141 acaatggctg gtacaacgag tcgcaagccg gtgacggcaa gctaatctct taaagccagt
     1201 ctcagttcgg attgtaggct gcaactcgcc tacatgaagt cggaatcgct agtaatcgcg
     1261 gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccacg
     1321 agagtttgta acacccgaag tcggtgaggt aaccgtaagg agccagccgc ctaaggtggg
     1381 atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg gctggatcac
     1441 ctcctttcta aggataagga actgcgcatt ggtcttgttt agtcttgaga ggtcttgtgg
     1501 ggccttagct cagctgggag agcgcctgct ttgcacgcag gaggtcagcg gttcgatccc
     1561 gctaggctcc attggtgaga gatcaccaag taatgcacat tgaaaattga atatctatat
     1621 caaatagtaa caagaaaata aaccgaaaac gctgtagtat taataagagt ttatgactga
     1681 aaggtcagaa aataaggtta agttaataag ggcgcacggt ggatgccttg gcactaggag
     1741 ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag ctgtaagtaa gcgatgatcc
     1801 agggatttcc gaatggggga acccaacagg taatacctgt tacccacatc tgttaaggat
     1861 gtgaggagga agacgcagtg aactgaaaca tctaagtagc tgcaggaaga gaaagcaaaa
     1921 gcgattgcct tagtagcggc gagcgaaacg gcagaagggc aaaccgaaga gtttactctt
     1981 cggggttgta ggactgcaat gtggactcaa agattataga agaatgattt gggaagatca
     2041 gccaaagaga gtaatagcct cgtatttaaa atagtctttg tacctagcag tatcctgagt
     2101 acggcgggac acgtgaaatc ccgtcggaat ctgggaggac catctcccaa ccctaaatac
     2161 tccctagtga ccgatagtga accagtaccg tgagggaaag gtgaaaagca ccccgggagg
     2221 ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt tcgagcccgt taatgggtga
     2281 gagcgtgcct tttgtagaat gaaccggcga gttacgttat gatgcgaggt taagttgaag
     2341 agacggagcc gtagggaaac cgagtctgaa tagggcgcct tagtatcatg acgtagaccc
     2401 gaaaccatgt gacctaccca tgagcaggtt gaaggtgcgg taagacgcac tggaggaccg
     2461 aaccagggca cgttgaaaag tgcttggatg acttgtgggt agcggagaaa ttccaaacga
     2521 acttggagat agctggttct ctccgaaata gctttagggc tagcgtcgac attagagatt
     2581 cttggaggta gagcactgtt tgggtgaggg gtccatcccg gattaccaat ctcagataaa
     2641 ctccgaatgc caatgaatta tggtcggcag tcagactgcg agtgctaaga tccgtagtcg
     2701 aaagggaaac agcccagacc accagctaag gtcccaaaat aattgttaag tggaaaagga
     2761 tgtggggttg cacagacaac taggatgtta gcttagaagc agctattcat tcaaagagtg
     2821 cgtaatagct cactagtcga gtgaccctgc gccgaaaatg taccggggct aaaacaattt
     2881 accgaagctg tggatacctt tataggtatg gtaggagagc gttctatgtg tgatgaaggt
     2941 ataccgtgag gagtgctgga acgcatagaa gtgagaatgc cggtatgagt agcgaaagac
     3001 aggtgagaat cctgtccacc gtaagactaa ggtttccagg ggaaggctcg tccgccctgg
     3061 gttagtcggg acctaaggag agaccgaaag gtgtatccga tggacaacag gttgatattc
     3121 ctgtactaga gtatgtagtg atggagggac gcagtaggct aactaaagca gacgattgga
     3181 agagtctgtc taagcagtga ggtgtgaatt gagtcaaatg cttaattcta taacattgag
     3241 ctgtgatggg gagcgaagtt tagtagcgaa gttagtgacg tcacactgcc aagaaaagct
     3301 tctagcgttt aaacatactc tacccgtacc gcaaaccgac acaggtagtc gaggcgagta
     3361 gcctcaggtg agcgagagaa ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg
     3421 ggagaagggg tgctgactta aagtcagccg cagtgaatag gcccaagcaa ctgtttatca
     3481 aaaacacagc tctctgctaa atcgtaagat gatgtatagg gggtgacgcc tgcccggtgc
     3541 tggaaggtta agaggagtgc ttagcgtaag cgaaggtatg aattgaagcc ccagtaaacg
     3601 gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc
     3661 gca
//
LOCUS       DAVKWS010000097         3651 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKWS010000097 DAVKWS010000000
VERSION     DAVKWS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.098"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(174..1724)
                     /locus_tag="VW852_002028"
     CDS             complement(174..1724)
                     /locus_tag="VW852_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV0273087.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLRLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1740..2519)
                     /locus_tag="VW852_002029"
     CDS             complement(1740..2519)
                     /locus_tag="VW852_002029"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV0273088.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2506..3357)
                     /gene="ylqF"
                     /locus_tag="VW852_002030"
     CDS             complement(2506..3357)
                     /gene="ylqF"
                     /locus_tag="VW852_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV0273089.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEPSVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
BASE COUNT         1081 a          759 c          697 g         1114 t
ORIGIN      
        1 gctttatact ttatcatttc taacttaatt atagtcttat ttttaataaa gttcaattat
       61 ttatatgtaa aattcctcat cagtaaaaaa taaccgatct catttctgag aatcggtttt
      121 ctaaataaat caagccaatc atttacataa tataaattat gtaaatgatt gtactacaac
      181 aacaaatctt tgacttttcc aatttcactt tttggaataa ccaggtgaat catatcaccc
      241 agatacattc tggttgagcc gttaactgtt tggctcttgc cattatggac ttgagttgtg
      301 atgaggacgt tgtgtggtaa gttgagttca tgaacttgtt tcccagcaat tttatcagaa
      361 actggtattt cgataagtgt aacttctcct tcgctagata cttcttctgg aagcattttt
      421 tccagcatgg cttcatagac tggcgtacct ttgagcaaat ccatgataat ataagaaaca
      481 agagtgacaa gaccaagtgg catgaggttg cgaatatctc ctaccatctc agttacgagg
      541 atcatagcgg ttaagggagc ttttgatatg gctccaaaat agccactcat tcctagaatg
      601 acaaatatag ggaattgctc ttgactgaca agtctaagat tgacacagat aacaccaatt
      661 aaggcaccaa gcaaagaacc aagagctaaa atggggagga aaattcctcc tggcagtcca
      721 cttccatagc taatcatact ccaaataaag cggatcaaaa agtaagctaa taaaacttgg
      781 aaactaaaat tttgttcagt taaagaaagg acaagctgat ttccgccacc aatgatttga
      841 ggtaagaaga ttccgactgg tatgataagg ataaaagcca agatgggata ataagctcta
      901 tccaaatgga ttttttgacc aatcaagtca taaactcttc caacgtttaa tacagctttc
      961 tcatagagaa agcctgaaaa tccaaggaaa attcccatga cgagatatat ccaatactga
     1021 tctagggtca tgggaggaat gttatctggc atatccaata ctggtgtcaa accgaacatg
     1081 agtagagaca caaagtttgc tacgatgctg gctgctagag ttgagaccca gaaaaagcgc
     1141 gaaaaatggt gatagacttc ttctacaaca aagagaagtg ctgcaatagg agcattaaag
     1201 gctgcggcta aacctgctgc agctccactg gcaatcaagg aacgttcctc tactggactg
     1261 gatttgagcc acttggcaat tcctttacca ccaactgctc caagttgaat actgggtccc
     1321 tctcgaccca gcatgagtcc actggcaata gcgagaatac ctagcacata ttttttccaa
     1381 agaatgcccc accagttgag ggacatgagg cctttcagtt cggcttcgac ttgaggaatt
     1441 cctgagcctt taatatcttt ttctgaccgt gttagtttgg cactgagcca acagatgagt
     1501 atataaaaca aaaccagtac aaaaagattg cgcactaagt acccttgatc ttgataaact
     1561 ccttgtatca tgtggaagcc cttttcaatt aagaaacgaa aggatccgac gataattcca
     1621 acgatgaggc cgacaatgat tcctcgacca acttgggata gtatagtgct ggatgcaaag
     1681 gcgaattctt tcttagaacg gactatttcc gactgttcct ccatggtatt tcctttcaat
     1741 taactttctt ttttacctaa aaccagtgat ttaacgggtt caaagctggt tcggtgaatt
     1801 ggggtaactc ctagttttgt gaggccttcc agatgtttag ctgtgccata tcctgcatta
     1861 gtagcgaaat catagccagg gaactgctga tcgtattctt tcatcaattc atcacgtgtt
     1921 accttggcta ctatagatgc tgctgcgata gagagggagt tggcatctcc tttgataatg
     1981 gaggtttgtg aaatgggcaa gtccagtttc atggcatcaa tcaaaaggtg ctctggttga
     2041 gggctgagct gggagattgc ttcttgcatg gctagtttgg ttgcttcata gatgttgact
     2101 tggtcgatga cctgattatc tatgatacca attccaatcg acaaggcttg gtcttgaacg
     2161 gcttggaaaa tctccagatg tttcttttta ggaattttct tgctgtcgtt gagaccttta
     2221 atcttacaat ttttaggtaa aataacggcc gcagcgacta caggaccagc aagaggacca
     2281 cgaccaacct catcaatacc tgcaattaag gtcaatcctt gcttataaag ttctttttca
     2341 taagaaagca tggattccaa gcgcaaattt tcatctaatt cagcttggat ggctctttta
     2401 cgcttgctga tttccttttg aactcctgag cgattatcct tttcaagctc taaaaaaata
     2461 gggctttcta actccttgac tgtcacaagg agttctttga tttctttaat cgttgccatc
     2521 gaggtcttcc aatgtatcta aggtatagtt accgagtttg ccatcacgga cttccttcac
     2581 gaagagactg taaaaacggt catagtcatc acggaaaccg agggcgcggg tcatatccat
     2641 aataatcaca gacggttctt cttcaatttt catttgtttg aagcgttcag ccagcttttc
     2701 tggataatgt tctttgaaat aattgatacc aaaaatggta acctcatcca taggaagcaa
     2761 ctggtctttg atagctccag tcaatgccaa cttaagtgca acagtttcat cctcaaactt
     2821 aggccagaga atccccggtg tatccaagat ttccaggtct ttattggttt taagccattg
     2881 ttgacctttt gtgacccctg gcttgtttcc aacaacagca atctttttac cagccaaacg
     2941 gttcatcaga gttgatttac cagcgtttgg aatcccgata atcatggtac gcaaggtttc
     3001 aatctgaatc ccacgttctt tctggcgagc aatcttatca gccatgagct tcttggccgc
     3061 atctgttaca acttttacag tcacttgctc tttggagttg atagctagcg tctggattcc
     3121 ttgtgattca aaatactgac gccattcctt ggtcattgct ggatcagcca agtcggcctt
     3181 gtttaaaatc aagagttttg gtttatcacc aacaatcttg gtcaacatag gattttgact
     3241 agatagaggc aagcgtgcat ctactaaaat cgtcacaaaa tcaacaaatt ttaaattctc
     3301 ctgcacctgt cgacgagctt tagacatgtg accaggaaac cattgaatag tagccattga
     3361 gttttttcct ttcgtagctc tgaagctgtt aatctagaac taagagatct caatctaaaa
     3421 atgaatgatg tccaaacagt tcttgtttta tgacataaaa atctacttta tattatatca
     3481 tgccttcttt attttgtccg atgatagcct tttaccctat atagaaaaaa gccatcaaat
     3541 gatgactttt cagagggagc aaaaaactgt cctctttatg ttttaaacat ttaacccaaa
     3601 tcattcatac ctctctcaac tagatgtaac ttacaaaacc cctgacctca t
//
LOCUS       DAVKWS010000098         3551 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKWS010000098 DAVKWS010000000
VERSION     DAVKWS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.099"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(62..1477)
                     /locus_tag="VW852_002031"
     CDS             complement(62..1477)
                     /locus_tag="VW852_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0273090.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1490..3328)
                     /locus_tag="VW852_002032"
     CDS             complement(1490..3328)
                     /locus_tag="VW852_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0273091.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3346..>3551)
                     /locus_tag="VW852_002033"
     CDS             complement(3346..>3551)
                     /locus_tag="VW852_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV0273092.1"
                     /translation="HRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYN
                     YLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1187 a          714 c          477 g         1173 t
ORIGIN      
        1 tattttatat ttaatgagat gttgccttaa ccgtgataat atctaatcat caataaacaa
       61 actatttgaa taaggattct ccatttgatt caatcactcc tttataccaa gtaaaggaca
      121 ttttcttata tcgattgaat gtgccatttc catcatcatt tcgatcaaca taaatgagac
      181 cgtacctttt agaaagttgt gcagtggaca tagaaacaca gtcaatacat ccccaagacg
      241 tatagcccat aatttcaaca ccatcctgta gagcttcagc aacttgcaat aaatgttctt
      301 tcatatactg aattctataa tcatcttgga cggttaagtt attaagttca tcttttatta
      361 gttgatcttt agcacctaat ccattttcta ctataaataa tgggatttga taacggtcat
      421 aatatctatt taaaattata cgtagtccaa ttggatcaat ttgccatccc cactctgaag
      481 actctaaata aggatttact aaaccaccaa taatattccc ttctcctgaa ttatactgtg
      541 ttggaatagc agattgagtc acactcatgt aatagctaaa ggataaaaaa tctacagtat
      601 aattttttaa taactctgca tcttcagctg caaactctat gttaatgtca tgttccttaa
      661 aatatctttt tgcataattc ggataataac ctctaacatg cacatctgaa aatagataat
      721 ttagattctc atactcatga gtcgcccata catcttttgg atttggagtc attggataag
      781 ctggcatagc taataccata caccccacct taaactctga attaatctca cgagcaattt
      841 ttgtaaccaa actcgaggcg actaattcat gatgtatagc ttgatataat tcttgtttcg
      901 aaagattctc cttaggtata tctattcctc cactagtaaa cggtaattcc aaaacagagt
      961 ttacttcgtt aaatgtaagc caatatttaa ctttatcttt atacctttct aaaactgttc
     1021 gagcaaattt ttcataaaaa tgaatcattc tcctatcaat ccatccatga tattttcttg
     1081 ctaaatataa tggagtctca tagtgtgaaa gagttacaag tggttctatc ccgtgagcat
     1141 gtagttcatc aaataattca tcataatatt tcaaaccagc ttcgttaggt tcttcctcat
     1201 ctccttttgg aaaaattcta ctccatgcaa tagaagtacg aaaaacatta aagcccattt
     1261 cagaaaacaa ggatatatct tccttatatt tatgataaaa atcaatacct atcaatttta
     1321 agttatcttc tgtaggattt tctgttgctt ctcctaatcc acctttgggt aacacatcct
     1381 gaactgataa gcccttacca tcttcattat atgctccctc tacttgatta gctgcaaccg
     1441 ctccacccca aagaaaatca tctggaaaaa tggtcataac tttcctccat tataatatta
     1501 ccagtaattc cttagaatgc tcgattgtct gattattagg taatactaat acatctagaa
     1561 aatcattggt attcgttaca attactggtg taactgtttc gtagccttta gtcttgatta
     1621 aattcaagtt catttcaaaa atcaactgat ttttgaaaac tctgtctcct tcttctacat
     1681 gactaataaa accttgacct tttagctcaa cagtatctaa tccaatatga attagtaact
     1741 caacaccctc atcactcttc aatccaattg cgtgcttagt cggaaaaata tttgtaattt
     1801 tcccatcaaa tggtgcataa accttacctt cacttgggat aatcgctact ccgtctccaa
     1861 ttaatttatc tgaaaatgtt ttatcctgga catcgcttaa cggaatgatt tctcctgata
     1921 taggagaaaa tatcattttt ttatttgaaa ctccagcttt aacttctaaa ttgctagaac
     1981 tctcttcttc atcgattcca aatatataag ctaatacaaa ggtaataaca accgaaatga
     2041 ccgccacaat taaagcattt acaatatttg atggcacatc agaataaata aattgaggca
     2101 acgctatcaa agatgggaca gcaaatagat atgctttaac actagtaaga cctgcaaata
     2161 atcccgctaa tccaccacca atcatagctg cataaagcgg ttttttatat tttaaagtca
     2221 caccatataa tgcaggttcg gtaatccctg caagtaaggc tgagaaacct gctgcaaaag
     2281 caatttgttt tgtattatta tttttactct ttaatgcaac agccatcgaa gcagcccctt
     2341 gagctaagtt tgaccctaac attgctggaa gaattaatac gtctggagta gcaatagatg
     2401 ccgccaaaaa aataggtgca aaagcccaat gcattccagt cataacaata aatggcataa
     2461 tagcaccaag aatagctaat gtaagccatc cagctacacc atacatttgc ccaactagat
     2521 ttgataatcc ttcaccaaca attactccaa taggtccgac tacaactaag gcaatacagc
     2581 ttgatactaa taatactagc gtaggttgca aaaaactctt agtaatagct ggtgttaatt
     2641 tagcaattat tttttcaata tatttcatca accaaaccat aataagaatt ggaacgactg
     2701 atgaaccata actagctggt gtcacaggtg caccaaataa actaagagga ttccctgatt
     2761 gcaccatttg aacaaaattt ggatggagaa gtacacctgc tacagacata gctaatgtag
     2821 atgttacttt taatttttgt gatgcagaat aagctaataa cagcggtaag aaataatatg
     2881 gagcatcccc aaaaaatgtc aaaaaagcaa tagtctgaga atctgattgc aatataccaa
     2941 gcattggtaa aatgattacc aagactttca acatacctcc ccctaacatt gctggaatga
     3001 ttggagtcat ggaaccagcg atatactcaa tgattctttc taaaatattc cctttgtgcc
     3061 cttgaacaac tgaatcggat tcaaaattgc caagtttaac gaattcttta taataattag
     3121 ctacatcatt accaagtata atttgatatt gtccattctt tttcataata cctattacat
     3181 ctggtatctt cttcacatca tcatcattga ctaaattttc atcttttaat tctaatctta
     3241 aacgtgttac acaatgggta actctattga catttttttc acctccaatt acatcgagga
     3301 ttttttgtac cgtatcttta taactcatgg tattctccta ttctattaat ctaaattttt
     3361 tgttaagcga cgaatatgag ccatcaaata aactaattca ctagaagtca gcaaataatt
     3421 gtactcagtt tgtataaaca ttgccacctg ttcaccacat tcatattctc taggatattt
     3481 atttttcatt aatgctaaca agtcttcatc atcatcgtca caataagttg tattttcaac
     3541 caagcgatgg g
//
LOCUS       DAVKWS010000099         3492 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKWS010000099 DAVKWS010000000
VERSION     DAVKWS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.100"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            18..>3492
                     /locus_tag="VW852_002034"
     CDS             18..>3492
                     /locus_tag="VW852_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0273093.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALPEAVVTDKG
                     EPAVQPELPEAVVTDKGEPEVQPALPEAVVSDKGEPEQVAPLPEYTRPQAGAVVEPAI
                     QPELPEAVVTDKGEPAVQPELPEAVVTDKGETEVQPESPDTVVSDKGEPKQVAPLPEY
                     TGPQASAIVEPEQVAPLPEYTGVQAGSIVEPEKVEAPKEYTGKIEQPSAEDTKPENEA
                     SSTNGESERPKDKIKEEKQVDKKLELRNVSNVELYTVENNKYRHITAVDGALDSSLKY
                     FMKVKSENFKDIMLPVTKIESTTKNNKEVYKIVAHAENLIQHENNVISNDYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSDPNGTFHLGATMDAREVELPDDQESYVKNEFYGKLIGE
                     NNGKYYAIYNLKKPLFKTLNTATIQNLSIKEANVSSKEDAATISKEAKYNTLIDNVHS
                     DGIIAGERGIGGLVSKVDNSRISNSSFTGRITNTYDTTVGYEIGGLVGKLSGSLASIE
                     KSIASIDIASNAKSGDQIVGGIAGVVEKSATIKYSYVEGNVNNVRHFGKVGGVAGNLW
                     DRDSQDVSKSGKLSYVLSDVNVTNGNAISGDNFDNMKEDHAYSSKGNKVVNVVQVDDE
                     LVTKDSDVQRGTVLDADKVKEKKAELVSKHSTKVEDFDFTSRYTTIYNEVTGYQKSRE
                     QVYKNIEKLLPFYNRETIVKYGNLVDESSELFTKELLSVVPMKNNEVITDINKNKQEI
                     NKLLLHFEGNKS"
BASE COUNT         1334 a          530 c          707 g          921 t
ORIGIN      
        1 tatttcaagg aggaataatg gaaaagtatt ttggtgaaaa acaagagcgt ttttcattta
       61 gaaaattatc agtaggactt gtatctgcaa cgatttcaag tttatttttt atgtctgtat
      121 tagctagttc atctgtggat gctcaagaaa ctgcgggagt tcactataaa tatgtggcag
      181 attcagagct atcatcagaa gaaaagaagc agcttgtcta tgatattccg acatacgtgg
      241 agaatgatga tgaaacttat tatcttgttt ataagttaaa ttctcaaaat caactggcgg
      301 aattaccaaa tactggaagc aagaatgaga ggcaagccct agttgctggt gctagcttag
      361 ctgctctggg aattttaatt tttgctgttt ccaagaaaaa ggttaagaat aaaacggtat
      421 tacatttagt attggttgcg ggaataggaa atggtgtctt agtttcagtc catgctttag
      481 aaaatcatct tttgctaaat tacaatacgg actatgaatt gacctctgga gaaaaattac
      541 ctcttcctaa agagatttca ggttacactt atattggata tatcaaagag ggaaaaacga
      601 cttctgattt tgaagtaagt aatcaagaaa aatcagcagc cactcctaca aaacaacaaa
      661 aggtggatta taatgttaca ccaaattttg tagaccatcc atcaacagta caagctattc
      721 aggaacaaac acctgtttct tcaactaagc cgacagaagt tcaagtagtt gaaaaacctt
      781 tctctactga attaatcaat ccaagaaaag aagagaaaca atcttcagat tctcaagaac
      841 aattagccga acataagaat ctagaaacga agaaagagga gaagatttct ccaaaagaaa
      901 agactggggt aaatacatta aatccacagg atgaagtttt atcaggtcaa ttgaacaaac
      961 ctgaactctt atatcgtgag gaaactatag agacaaaaat agattttcaa gaagaaattc
     1021 aagaaaatcc tgatttagct gaaggaactg taagagtaaa acaagaaggt aaattaggta
     1081 agaaagttga aatcgtcaga atattctctg taaacaagga agaagtttcg cgagaaattg
     1141 tttcgacttc aacgactgcg cctattccaa gaatagtcga aaaaggtact aaaaaaactc
     1201 aagttataaa ggaacaacct gagactggtg tagaacataa ggacgtacag tctggagcta
     1261 ttgttgaacc cgcaattcag cctgagttgc cagaagctgt agtaagtgat aaaggcgtac
     1321 cagaagttca accagcgtta ccggaagcag tcgtaaccga caaaggtgag cccgctgtcc
     1381 aacctgaatt acccgaagct gttgtaactg acaaaggcga gcctgaagtt caaccagcgt
     1441 tacccgaagc ggttgtaagt gataaaggtg aaccagagca ggtagcccca ttgccagaat
     1501 atacaagacc acaagcaggt gcagtagttg aacctgcaat ccaaccagag ttaccagaag
     1561 ctgttgtaac tgacaaagga gaaccagcag tccaaccaga gctaccggaa gcggttgtaa
     1621 ctgacaaagg tgagactgag gttcaaccag agtcgccaga tactgtggta agtgataaag
     1681 gtgaacctaa acaggtagca ccgcttccag aatatacagg accacaagcc agcgctattg
     1741 ttgaaccaga gcaagtagca ccactaccag aatacacagg ggtacaagcc ggatcgatag
     1801 tcgagccaga gaaagtagaa gcaccgaaag aatatactgg taaaatcgaa caacctagtg
     1861 cagaagatac taaacctgaa aatgaggcaa gctctacaaa tggggaatcg gaacgtccta
     1921 aagataaaat taaagaagaa aaacaagtcg acaagaaact agaacttaga aatgtttcaa
     1981 acgtggaatt atatacggta gaaaataata aatatcgaca tattacggct gtagatggtg
     2041 ctttagatag ttcattgaag tatttcatga aggtaaaatc agaaaatttt aaagatataa
     2101 tgttgcctgt tactaaaatt gaaagtacaa caaaaaacaa taaagaagta tacaaaattg
     2161 tggctcatgc agaaaatcta attcaacacg aaaacaatgt tatttcgaat gactatacat
     2221 attatttacc aaaaactcag caaagtgaaa ctggcgtcta tacatcattt aaaaatttag
     2281 ttgatgcaat gaatagtgac cctaatggaa cattccattt aggtgcaact atggatgctc
     2341 gtgaagttga acttccggat gatcaagaaa gttatgtaaa aaatgaattt tacggaaaat
     2401 taataggtga aaataatggt aagtattatg ctatttataa cttgaaaaaa cctttattta
     2461 aaactctaaa caccgctact attcagaatc tttcaataaa agaagcgaat gtttcttcta
     2521 aagaagatgc tgctacaata tcgaaagaag caaaatacaa tactcttata gataatgttc
     2581 attctgatgg tattatagct ggtgaacgtg gaattggtgg attggtttca aaagtagata
     2641 actcgagaat ttctaatagt agttttacag gcagaataac taacacatat gatacaactg
     2701 tagggtatga aattggtggt ttagtaggta aactttctgg ttcgcttgct tctatagaga
     2761 aatcaatagc atcgattgac atagcctcta atgcaaaatc aggagatcaa attgttggtg
     2821 gtatagccgg agttgttgag aaaagtgcga caattaagta tagctatgtt gaaggaaatg
     2881 ttaacaatgt gcgacacttt ggtaaagttg ggggagttgc aggtaatctt tgggatagag
     2941 attcacaaga tgtaagtaaa tcaggtaaac tatcatatgt tttaagtgat gttaatgtaa
     3001 caaatggtaa tgctatatcg ggtgataatt ttgataatat gaaagaagat catgcttata
     3061 gtagtaaagg caataaagta gtaaatgtag tccaagtaga tgatgaactt gtgactaaag
     3121 attcagatgt acaaagaggt acggtattag atgctgataa agtaaaagag aaaaaggcag
     3181 aattggtttc caaacacagc actaaagtag aagattttga tttcacttca agatacacta
     3241 ctatttacaa tgaagtaaca ggttatcaaa agtcgagaga acaagtatat aaaaatattg
     3301 aaaaactatt accgttctat aatcgagaga caattgttaa atatggtaat ttagtagatg
     3361 aaagcagcga gctatttact aaagaacttc tatcagttgt tcctatgaag aataatgaag
     3421 taataactga tattaataaa aataaacaag aaataaacaa attgttatta cattttgaag
     3481 gtaataaatc ac
//
LOCUS       DAVKWS010000100         3437 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKWS010000100 DAVKWS010000000
VERSION     DAVKWS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.101"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            313..2382
                     /locus_tag="VW852_002035"
     CDS             313..2382
                     /locus_tag="VW852_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0273094.1"
                     /translation="MKLTKVIINNFRSFGESQIIELNNQTVLIGNNSSGKTTVLQALS
                     KLFSDKQNDRIIKKSDFHLPKGSRPGENTRNLFIETIFEFDELDGTPYSQAIPSFFEH
                     FTVSKDGAKPFLRIRLESSWEDDGTVEGSIDTQFYYISSDEDTIRDEDKHRAPRKDLD
                     KIRVLYVPASRTPEKELGNASGSMLSRLVNSINWTEDEINEITDKIDELNNTFLSENG
                     ALTQINNEIQKSWELYHEDNRFSQAELIINSSEMAAALRQIALKFSPTTTEEAFTVSD
                     LGDGLRSIFYFSLVDSILDIELEITKDREENPDNPRFKLIPPVLTILAIEEPENHIAP
                     HHIGKLIKRFKQLGNNDNSQVILTSHSPAIVKRIDPEDLKYLRIENNDRVLQTIVSDI
                     QLPPAIDESYKYIKGAIQAYPELYFAKLVVLGEGDSEELLLPKFFDLLGKEVDSSQIS
                     IVPLGGRHVNYFWKLLNALRIPHITLLDFDNERYGGGWGRIKYISQHLYELNTEFQEW
                     FHTQRLDFNEIGSRGCESIGAQRLINWFNKLEEFNVYFSSPLDIDFLMLQHYKDNYLN
                     TLSPIEGPVVSYNDSDGSSKKVKLSDLDCLDRLQLEGFNKRKEEAIRATLKDKSGHGD
                     SFNEEEKELMIWYQYFFLGRGKPTTHMQFLSSISDDELTRNLPPVFEKIVNSAEELLG
                     DIQHGKE"
     gene            2369..>3437
                     /locus_tag="VW852_002036"
     CDS             2369..>3437
                     /locus_tag="VW852_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV0273095.1"
                     /translation="MVKSEEWFPKGDIILEETALGAVKDVTNCLVIAGPGAGKTELLA
                     QKLDYLFSTNKCVSPKKILALSFKTDAASNLKERVKKRYGDEYASRFTSLTYSAFEKR
                     ILDQFRDVLPEDIRPSRDYLIEDWYTIKELLSMNGINVNGWRMSDIRRYVENIILNNG
                     DNHKFKTDLLKGTQDNKPVLLYRQITKLSTQIIDTNEYIRKALQMTYDFVFLDEFQDT
                     TYAQYDLLKTCFLGSSCKLTAVGDDKQAIMRWAGAKPDIFPDYIRDFNPNEYQLLMNH
                     RSVPKLVEFQKEVHQILNSNHSSIQTNNYPEFQEGEITLFEFENESLEAKLIANDIES
                     KIQGGIRPSEICILAKQKVGIY"
BASE COUNT         1247 a          485 c          657 g         1048 t
ORIGIN      
        1 aaagagcatg ctataattgg taacaatata aaattctacg aaagacacat ggcctgacaa
       61 aaacagattt gctcgaattg tataaatttt acgcaatagc ccttagctgt tataaaaacg
      121 gaacaagtac tatttcaact atttgctagg aattcataga agatgtgtct atgtttcaaa
      181 aatagatcaa ggttaagctt ctgtcactct tcaataatgc ctttttgggt aatagagtag
      241 ggttgaggaa gagatgtaga aggtgggaca ggaatggttg tgtgaattag tttcaggtaa
      301 tggagataag aaatgaagtt aacaaaagta attattaata attttagatc attcggtgaa
      361 agtcaaatta ttgaattaaa caatcaaact gtactgattg ggaataatag ttcgggaaaa
      421 acaactgtat tacaagcatt gagtaagctg ttttcggaca aacaaaatga tagaataata
      481 aaaaaaagtg attttcacct tccaaaaggt tcaaggcccg gagaaaatac tagaaacctc
      541 tttatagaaa ctatttttga gtttgatgaa cttgatggaa ctccctatag tcaagcaatt
      601 ccatcgtttt tcgaacactt tacagtttct aaagatggtg ccaaaccatt tctacgaatc
      661 aggttagaat cttcttggga agatgatgga acagttgaag gaagtattga tactcaattc
      721 tattatattt catctgatga agatactatt agagatgaag acaaacatcg tgctcctaga
      781 aaggatttgg ataaaattag ggtactttat gtacctgctt caagaacgcc tgagaaggag
      841 ttgggtaatg cttcaggtag tatgttaagt aggttggtaa atagtataaa ttggaccgaa
      901 gatgagataa atgaaattac agataaaatt gatgagttga ataacacctt tttatctgaa
      961 aatggagcat tgactcagat caacaatgag attcagaagt catgggaatt atatcatgaa
     1021 gataaccgtt tttctcaagc agagttgatt attaattctt ctgagatggc ggcagcactt
     1081 agacagattg ccttaaaatt ttcaccaaca accactgaag aagcatttac agtttcagat
     1141 ctaggagatg gactgagatc tattttttat ttttcacttg ttgattcgat acttgatatt
     1201 gagttagaaa ttaccaaaga ccgtgaagaa aatcctgata atccaagatt taagttgatt
     1261 ccaccagtac taactatcct agcaatagaa gaaccagaaa atcatattgc tccacatcac
     1321 attggaaaac tgataaaaag gtttaagcaa ttaggcaata atgacaactc ccaagtaatt
     1381 ttaacgtcac attctccagc tattgtaaaa agaattgacc cagaagattt gaagtattta
     1441 agaatagaaa ataatgacag agttcttcaa accattgttt ctgatataca gctacctcca
     1501 gctatagatg aatcttataa atatattaaa ggagcaattc aagcttatcc tgaactatat
     1561 tttgcgaagt tagtagttct aggtgaaggc gacagtgagg aactgctatt accaaagttt
     1621 tttgatttac ttgggaaaga ggtagatagc tctcagattt caattgtccc tttagggggc
     1681 agacatgtca actatttttg gaaattattg aatgctctca gaattcctca tattaccttg
     1741 ttggattttg ataatgagag atacggaggt ggttggggaa gaataaaata catttctcaa
     1801 catctgtatg agctgaacac agagtttcaa gaatggttcc atactcagag acttgatttt
     1861 aatgaaattg ggtcacgagg ttgtgaatcg ataggagctc aacgtctgat taattggttt
     1921 aataaactcg aagaatttaa tgtctacttt tcatcgcctt tagatattga tttcttgatg
     1981 ctacaacatt ataaagacaa ttatctgaat actttatccc caatagaagg accagttgtt
     2041 tcatataatg attcggatgg aagtagtaaa aaagtgaaat tgagtgattt agactgtctt
     2101 gatagattgc agttagaagg tttcaacaaa cgtaaagagg aagcaataag agctacttta
     2161 aaagataaga gtggacatgg cgatagtttc aatgaagaag aaaaagaatt aatgatttgg
     2221 tatcaatatt tctttttagg tagaggaaaa ccaacaactc atatgcaatt tttatcatct
     2281 attagtgatg atgaattaac aagaaatcta ccccctgtat ttgaaaaaat agttaacagt
     2341 gctgaagaat tacttggaga tattcaacat ggtaaagagt gaggagtggt ttcctaaagg
     2401 agatatcatt ttagaagaga ctgcattagg agccgtcaaa gatgtgacta actgtttagt
     2461 cattgctgga ccaggtgctg ggaaaactga attactagct caaaagttag actatctatt
     2521 ctctacaaat aaatgcgttt ctccgaaaaa aattctagca ttaagtttca aaacggatgc
     2581 tgcatcaaat ttgaaagaaa gagttaagaa acgttatggt gatgaatatg cttctcgttt
     2641 tacctcatta acctattcgg cttttgaaaa gagaatttta gaccaattta gagatgttct
     2701 acctgaggat atacggccat caagagatta tttgattgaa gattggtata ctataaaaga
     2761 actactctct atgaatggaa ttaatgttaa tggatggaga atgtcagata taagaagata
     2821 tgtcgaaaac atcatactta acaatgggga taatcacaag tttaaaactg acttactaaa
     2881 aggaacacaa gataataagc cggtcctctt gtataggcaa ataacaaagt tgagtactca
     2941 aataatagat actaacgaat acatccgtaa agcacttcaa atgacatacg attttgtatt
     3001 tctagatgaa tttcaggata ctacatatgc ccagtatgat ttattaaaaa cttgtttttt
     3061 aggttcgtct tgtaaattaa cagcagttgg agatgataag caagccatta tgagatgggc
     3121 aggtgcaaag cctgatattt ttccagacta tattcgagac tttaatccaa atgaatatca
     3181 attgttgatg aatcaccgct ctgtacctaa gcttgtagaa tttcaaaaag aggttcatca
     3241 gatattaaat agtaatcaca gttctattca gacaaataat tatccagaat ttcaagaggg
     3301 tgaaataaca ttatttgaat ttgaaaatga aagtttagaa gctaagttaa ttgcaaatga
     3361 tatagaatca aaaattcaag gaggtatacg accatcagaa atttgtatcc tagcaaaaca
     3421 aaaggttggt atttata
//
LOCUS       DAVKWS010000101         3330 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKWS010000101 DAVKWS010000000
VERSION     DAVKWS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.102"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            48..3167
                     /gene="phtE"
                     /locus_tag="VW852_002037"
     CDS             48..3167
                     /gene="phtE"
                     /locus_tag="VW852_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV0273096.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1241 a          545 c          646 g          898 t
ORIGIN      
        1 aaaagaagtt gagaaaaaac ttcatcattt attgaaatga gggatttatg aaatttagta
       61 aaaaatatat agcagctgga tcagctgtta tcgtatcctt gagtctatgt gcctatgcac
      121 taaaccagca tcgttcgcag gaaaataagg acaataatcg tgtctcttat gtggatggca
      181 gccagtcaag tcagaaaagt gaaaacttga caccagacca ggttagccag aaagaaggaa
      241 ttcaggctga gcaaattgta atcaaaatta cagatcaggg ctatgtaacg tcacacggtg
      301 accactatca ttactataat gggaaagttc cttatgatgc cctctttagt gaagaactct
      361 tgatgaagga tccaaactat caacttaaag acgctgatat tgtcaatgaa gtcaagggtg
      421 gttatatcat caaggtcgat ggaaaatatt atgtctacct gaaagatgca gctcatgctg
      481 ataatgttcg aactaaagat gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg
      541 agaaggttaa ctctaatgtt gctgtagcaa ggtctcaggg acgatatacg acaaatgatg
      601 gttatgtctt taatccagct gatattatcg aagatacggg taatgcttat atcgttcctc
      661 atggaggtca ctatcactac attcccaaaa gcgatttatc tgctagtgaa ttagcagcag
      721 ctaaagcaca tctggctgga aaaaatatgc aaccgagtca gttaagctat tcttcaacag
      781 ctagtgacaa taacacgcaa tctgtagcaa aaggatcaac tagcaagcca gcaaataaat
      841 ctgaaaatct ccagagtctt ttgaaggaac tctatgattc acctagcgcc caacgttaca
      901 gtgaatcaga tggcctggtc tttgaccctg ctaagattat cagtcgtaca ccaaatggag
      961 ttgcgattcc gcatggcgac cattaccact ttattcctta cagcaagctt tctgccttag
     1021 aagaaaagat tgccagaatg gtgcctatca gtggaactgg ttctacagtt tctacaaatg
     1081 caaaacctaa tgaagtagtg tctagtctag gcagtctttc aagcaatcct tcttctttaa
     1141 cgacaagtaa ggaactctct tcagcatctg atggttatat ttttaatcca aaagatatcg
     1201 ttgaagaaac ggctacagct tatattgtaa gacatggtga tcatttccat tacattccaa
     1261 aatcaaatca aattgggcaa ccgactcttc caaacaatag tctagcaaca ccttctccat
     1321 ctcttccaat caatccagga acttcacatg agaaacatga agaagatgga tacggatttg
     1381 atgctaatcg tattatcgct gaagatgaat caggttttgt catgagtcac ggagaccaca
     1441 atcattattt cttcaagaag gacttgacag aagagcaaat taaggctgcg caaaaacatt
     1501 tagaggaagt taaaactagt cataatggat tagattcttt gtcatctcat gaacaggatt
     1561 atccaagtaa tgccaaagaa atgaaagatt tagataaaaa aatcgaagaa aaaattgctg
     1621 gcattatgaa acaatatggt gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg
     1681 cgattattta tccgcatgga gatcaccatc atgcagatcc gattgatgaa cataaaccgg
     1741 ttggaattgg tcattctcac agtaactatg aactgtttaa acccgaagaa ggagttgcta
     1801 aaaaagaagg gaataaagtt tatactggag aagaattaac gaatgttgtt aatttgttaa
     1861 aaaatagtac gtttaataat caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt
     1921 ttagttttcc gcctgaattg gagaaaaaat taggtatcaa tatgctagta aaattaataa
     1981 caccagatgg aaaagtattg gagaaagtat ctggtaaagt atttggagaa ggagtaggga
     2041 atattgcaaa ctttgaatta gatcaacctt atttaccagg acaaacattt aagtatacta
     2101 tcgcttcaaa agattatcca gaagtaagtt atgatggtac atttacagtt ccaacctctt
     2161 tagcttacaa aatggccagt caaacgattt tctatccttt ccatgcaggg gatacttatt
     2221 taagagtgaa ccctcaattt gcagtgccta aaggaactga tgctttagtc agagtgtttg
     2281 atgaatttca tggaaatgct tatttagaaa ataactataa agttggtgaa atcaaattac
     2341 cgattccgaa attaaaccaa ggaacaacca gaacggccgg aaataaaatt cctgtaacct
     2401 tcatggcaaa tgcttatttg gacaatcaat cgacttatat tgtggaagta cctatcttgg
     2461 aaaaagaaaa tcaaactgat aaaccaagta ttctaccaca atttaaaagg aataaagcac
     2521 aagaaaactc aaaacttgat gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa
     2581 aagaaaaact ttctgaaact gggaatagta ctagtaattc aacgttagaa gaagttccta
     2641 cagtggatcc tgtacaagaa aaagtagcaa aatttgctga aagttatggg atgaagctag
     2701 aaaatgtctt gtttaatatg gacggaacaa ttgaattata tttaccatcg ggagaagtca
     2761 ttaaaaagaa tatggcagat tttacaggag aagcacctca aggaaatggt gaaaataaac
     2821 catctgaaaa tggaaaagta tctactggaa cagttgagaa ccaaccaaca gaaaataaac
     2881 cagcagattc tttaccagag gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg
     2941 ataatggaat gttgaatcca gaagggaatg tggggagtga ccctatgtta gatccagcat
     3001 tagaggaagc tccagcagta gatcctgtac aagaaaaatt agaaaaattt acagctagtt
     3061 acggattagg cttagatagt gttatattca atatggatgg aacgattgaa ttaagattgc
     3121 caagtggaga agtgataaaa aagaatttgt ctgatctcat agcgtaagga atagcagtag
     3181 aaaaagtctg aatcaaaaat gaagttctct caaaagttag aaataaaact ctgactttgg
     3241 gagaatttca ttttattatt aatatataaa atttcttgac atacaactta aaaagaggtg
     3301 gaatatttac tagttaatta attggttaat
//
LOCUS       DAVKWS010000102         3248 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKWS010000102 DAVKWS010000000
VERSION     DAVKWS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.103"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            15..209
                     /locus_tag="VW852_002038"
     CDS             15..209
                     /locus_tag="VW852_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0273097.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(575..1885)
                     /locus_tag="VW852_002039"
     CDS             complement(575..1885)
                     /locus_tag="VW852_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV0273098.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2122..2994
                     /locus_tag="VW852_002040"
     CDS             2122..2994
                     /locus_tag="VW852_002040"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV0273099.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3125..>3248
                     /locus_tag="VW852_002041"
     CDS             3125..>3248
                     /locus_tag="VW852_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0273100.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKL"
BASE COUNT         1008 a          541 c          732 g          967 t
ORIGIN      
        1 tacgaataat atagatgaag gggaaagagt atgaaaacag aactgtttct tttgctatta
       61 gttcaaaagg agaaaaaatg aaagtagaaa atatttcgta tagggtggat catcgtatat
      121 tgtttgataa tatttctttt gatacttcga gttcaggcgt gacattaatt actggtaaaa
      181 atggtacagg aaagtcaact ttactatagt agattgaaac tagaatagta cacatctact
      241 tctaaaatat tgttagaaat tgatttgact atcctgatct atttgtcctg ttcttatttc
      301 atttcactat atctcaaatt gagtatgacg aagtgcgctc ccatgtcctg ggaacgcact
      361 ttcttcatat ttttcatatt cttgaatcca tcgataaaga ctattgggat gaatttttaa
      421 agttgaacta atcattttta caggatgaga tttacagcag agagtttgaa ggctttatca
      481 aaggtttttc ttggcataat gacttttcct cgtttccact taattttgtg tctactttat
      541 tataccaagt ccacccttaa gttagataat aaatctaact taaggaagct agaaggatga
      601 gaatccaggt ggtcaagagt cccaaactta agctgatggg gacacccaga ataatttgct
      661 ttttgaaggc aaggccacgt tcctctatat tgggaagtga gagttgaatg agagaaccag
      721 ctgatgaaaa gggtgagata ttagtagata gagcgccaat aacggtggct gttgtgagta
      781 agtgaatatc aatctgagga ttttgagcac tgatgatagc aatgatggga aagagggctg
      841 gagctacaac ggatagggtg gaactaaaga gtgacatcac tccggctatc acacaaaaga
      901 acagaggtaa ccagaaatga ggaatggttg ttgtcatgag gtgccctatc agtgtgacta
      961 aacctgactt gaccgctaga gacattagta agctcatgcc gcagagcatg ataattgtag
     1021 cccagggaac cttagctaaa atggcttctt gcttccctaa tttgagcctt aaggcgaggc
     1081 agaccatgag tattgagaca aagccaatat caaatgtttt ttgataagta gctatccagg
     1141 cgatgtttgg gaaaatgaga tgcaacaagg gaaaaagcca aaccaaaacc atgctgctga
     1201 tcatgagcaa ggtggtttgt ctttgaacct tgctgaggag tggtggttgg tcaatagtca
     1261 aggatgagtt tgttcttccc ttactatagt gactgtaaca ggataataaa agcaagacga
     1321 tgagtgggta gataatgctg acgataaaga tatgattgcc aagtgaaaaa gcttgctcct
     1381 cccatcccat ttgcttaaac aggccttgaa agacaatgcc tgagctactg gttatcaaat
     1441 tagcccctcc tgaagctccc caattgacgg cttgagctcc aatcaaaggg tgtttgtccg
     1501 ctttttgaca gagggtaatc gctagaggac agcaaacggc catagtagtg aaaaatccag
     1561 cacctaaagc agacaaaagg gttgccatca ggtataaaat catgtagagg gcgttagggt
     1621 gggtgcgtgt gcggtagaga atgtgttgag ccaaaacatc aagagtaccg ttagttgttg
     1681 caacgttata aaagagagag acgctaaaaa tggtaaaaaa gagtgaggtt ggccaaaaat
     1741 gaagaagttc tttggggctt aatcccatga gagtggttgc gatgaggtaa gaaaaagcaa
     1801 tagccagcag gccaatattg attttggtgc ggtaaccaat tccaatggct agagcaatgg
     1861 cgctaatcat tattaaatga atcattgaat tgtcctttca gttagaatat ggaaataggt
     1921 tggcaatttt ggcgctcaaa agaccacctg tttcctattg gtacttgact attatacaca
     1981 aaaaaaatac aattgtcttg acaattgtat tgacacttgt tagaattaaa aatgaggtat
     2041 tcaggcttgt gatttcctat ggaagttaat agtaattgcc tctagtgctt acaagtgata
     2101 ttaaaaatag aggacttagt gatgtcaatc atttcaactg atttaacccc ttttcaaata
     2161 gatgatacat tgaaagcagc cttgcgagaa gatgttcatt ccgaagatta cagtaccaat
     2221 gccatttttg atcatcatgg ccaagccaag gtgtcgcttt ttgccaagga agctggtgtt
     2281 ttagcgggac taaccgtttt tcaaagggtt tttaccctat ttgatgccga ggtgaccttc
     2341 cagaatcctc atcaatttaa ggatggggat cgtttgacta gtggcgattt ggttttagaa
     2401 atcataggct cggtgagaag tctcttaaca tgtgaacgcg ttgccttgaa ttttttacaa
     2461 catttatcag ggattgcttc gatgacagct gcttatgtag aagccttagg cgatgattgc
     2521 attaaggtat ttgatactcg aaaaactact cctaatttac gtctttttga gaaatatgcc
     2581 gtgagagttg gcggtggcta taatcatcgc tttaatttat cagatgctat catgctaaaa
     2641 gacaatcaca ttgcggcagt aggtagtgtt caaaaggcaa ttgctcaagc gcgtgcctat
     2701 gccccttttg tgaaaatggt cgaggtggaa gtggaaagcc ttgctgctgc cgaagaagct
     2761 gcggcggcgg gtgctgatat tatcatgttg gataatatgt cattggaaca gattgaacag
     2821 gccattaccc taattgcagg acgttctcgg attgaatgtt ctggaaatat tgatatgacc
     2881 actattagcc gttttcgtgg tttagcgatt gattacgtct ccagtggtag tttaacccat
     2941 agtgctaaga gtcttgattt ttccatgaag ggtttaacct accttgatgt ctaagttgta
     3001 aaataaacta actttttaaa ggatgtcttt cctctagaac gagttttatg tcagatagtt
     3061 taaacgcctc ttcaaatata gtaaaatgaa ccaaaaatag tacaaaatgt ggtataatct
     3121 tcttatggca tattcaatag attttcgtaa aaaagttctc tcttattgtg agcgaacagg
     3181 tagtataaca gaagcatcac acgttttcca aatctcacgt aataccattt atggctggtt
     3241 aaagctaa
//
LOCUS       DAVKWS010000103         3107 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKWS010000103 DAVKWS010000000
VERSION     DAVKWS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.104"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            298..1584
                     /locus_tag="VW852_002042"
     CDS             298..1584
                     /locus_tag="VW852_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0273101.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1599..1910
                     /locus_tag="VW852_002043"
     CDS             1599..1910
                     /locus_tag="VW852_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273102.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            2170..2898
                     /locus_tag="VW852_002044"
                     /pseudo
     CDS             2170..2898
                     /locus_tag="VW852_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          962 a          541 c          676 g          928 t
ORIGIN      
        1 atattctaga ctgtatcaat ccttttccat tataggagca acagagtggg agtagtcatc
       61 taaggactaa tttatgtatt tttacgagtc agtatcttgg gatactggtt tttacttttt
      121 tagacttttt gactacttgt taaaactggg gtaattttcg actgtttaac agttattatg
      181 caaagtctaa aagattagaa ttgtcaaaac aatccgtcta ggcttgattt tatcctttat
      241 ttactataaa atgagaagga aaaatgtcaa acttttatat tgcaaatagg agaaatcatg
      301 acaaaaacat taaaacgtcc tgaggtttta tcacctgcag ggactttaga gaagctaaag
      361 gtagctgttc agtatggagc agatgctgtc tttatcggtg gtcaggccta tggtcttcgt
      421 agccgtgcgg gaaactttac tttcgaacag atggaagaag gcgtgcagtt tgcggccaag
      481 tatggtgcca aggtctatgt agcggctaat atggttatgc acgaaggaaa tgaagctggt
      541 gctggtgagt ggttccgtaa actgcgtgat atcgggattg cagcagttat cgtatctgac
      601 ccagccttga ttatgattgc agcgactgaa gcaccaggcc ttgaaatcca cctttctacc
      661 caagccagtg ccactaacta tgaaaccctt gagttctgga aagagctagg cttgactcgt
      721 gtcgttttag cgcgtgaggt ttcaatggaa gaattagctg agatccgcaa acgtacagat
      781 gttgaaattg aagcctttgt ccatggagct atgtgtattt catactctgg acgttgtact
      841 ctttcaaacc acatgagtat gcgtgatgcc aaccgtggtg gatgttctca gtcatgccgt
      901 tggaaatacg acctttacga tatgccattt gggaaagaac gtaagagttt gcagggtgag
      961 attccagaag aattttcaat gtcagccgtt gacatgtcta tgattgacca cattccagat
     1021 atgattgaaa atggtgtgga cagtctaaaa atcgaaggac gtatgaagtc tattcactac
     1081 gtatcaacag taaccaactg ctacaaggcg gctgtggatg cctatcttga aagtcctgaa
     1141 aagtttgaag ctatcaaaca agacttggtg gacgagatgt ggaaggttgc ccaacgtgaa
     1201 ttggcaacag gtttctacta cggtatacca tctgaaaatg agcagttgtt tggtgctcgc
     1261 cgtaaaattc ctgagtacaa gtttgtcgct gaagtggttt cttatgatga tgcggtacaa
     1321 acagcaacca ttcgtcaacg aaacgtcatt aacgaagggg accaagttga gttttatggt
     1381 ccaggtttcc gtcattttga aacctatatt gaagatttgc atgatgccaa aggaaataaa
     1441 atcgaccgcg ctccaaatcc aatggaacta ttgactatta aagtcccaca acccgttcaa
     1501 tcaggagaca tggttcgtgc attaaaagaa ggactcatca atttttataa ggaagatgga
     1561 accagcgtca cagttcgagc ttaagaaagg aaaaggaaat gatagaggca cagggtttct
     1621 tagtggataa gcaaacaaga tgcattcatt accatagcaa gctggatatt attgctttac
     1681 aatgctatga ttgtaaaaag tattatgctt gttatcggtg tcatgattca ttagaaaatc
     1741 acccttttga gccttatccc ttatctttga tacaggataa gcctatttta tgtggtgttt
     1801 gtctaaaact attaacatat aagcaatata aagaaagctt aagttgcctc ttttgttttt
     1861 ctcgctttaa tccaggttgc caaaatcata aggaacgcta ttttaaatag caaatcatct
     1921 agttttgaag taggagaaaa ctcaattcca agagaaaatg aagtaaatct tcccacaata
     1981 aaacgcataa tatcaagatt gttcaatacc tgatactatg cgtttttaag attttaaaga
     2041 cttttttcct agcccctttt tactttatct ggtattttga ctacttgtta aaactggggt
     2101 aattttcgac tgtttaacag ttattatgca aagtctaaaa ggttagaatt gtcaaaacaa
     2161 tccgtctaga gtatgcgtga tgccaaccgt ggtggatgtt ctcagtcatg ccgttggaaa
     2221 tacgaccttt acgatatgcc atttgggaaa gaacgtaaga gtttgcaggg tgagattcca
     2281 gaagaatttt caatgtcagc cgttgacatg tctatgattg accacattcc agatatgatt
     2341 gaaaatggtg tggacagtct aaaaatcgaa ggacgtatga agtctattca ctacgtatca
     2401 acagtaacca actgctacaa ggcggctgtg gatgcctatc ttgaaagttc tgaaaagttt
     2461 gaagctatca aacaagactt ggtggacgag atgtggaagg ttgcccaacg tgaactggtt
     2521 acaggatttt actatggtac accatctgaa aatgagcagt tgtttggtgc tcgttgtaaa
     2581 attcctgagt acaagtttgt cgctgaagtg gtttcttatg atgatgcgac acaaacagca
     2641 accattcgtc aacggaacgt cattaacgaa ggggaccaag ttgagtttta tggtccaggt
     2701 ttccgtcatt ttgaaaccta tattgaagat ttgcatgatg ccaaaggcaa taaaatcgac
     2761 cgcgctccaa atccaatgga actattgact attaaagtcc cacaacttgt tcaagctgga
     2821 gacatggttc gagctcttaa agaggggctt atcaatcttt ataaggaaga tggaaccagc
     2881 gtcacagttc gtgcttaatg tagttgttta gttttaaaaa attatgcaaa gctccatata
     2941 caacacttaa acgagattaa agaatggcga aatcccttga tgcgcaagag attagctgtc
     3001 cttttttatt ttttaagtga taaagtcgga gcttaggtat taaagcctat caaattaaac
     3061 aaagaagcga tgtcttagat attttgaaaa aaattaataa gcagaaa
//
LOCUS       DAVKWS010000104         3080 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKWS010000104 DAVKWS010000000
VERSION     DAVKWS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.105"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(283..816)
                     /locus_tag="VW852_002045"
     CDS             complement(283..816)
                     /locus_tag="VW852_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV0273103.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(905..1681)
                     /gene="recX"
                     /locus_tag="VW852_002046"
     CDS             complement(905..1681)
                     /gene="recX"
                     /locus_tag="VW852_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV0273104.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1719..3074
                     /gene="rlmD"
                     /locus_tag="VW852_002047"
     CDS             1719..3074
                     /gene="rlmD"
                     /locus_tag="VW852_002047"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0273105.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVSKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          979 a          540 c          552 g         1009 t
ORIGIN      
        1 aaaatgtttt tgatacgaag ttgacctgta ttttttatac taatcatttt cgtatttttt
       61 gtattaaacg atataagttt gttgtaaact tacaaggaat aaagacatta aaaaataaca
      121 gtatatctat ttgttttata tattttacga attttgtata aatctctttc tagtaatgtg
      181 ttgtaactct gctataatag atttattcct ttttgtgttt acacaattta ttttatacta
      241 ccaaaaaaaa ggtcaggatt ttgttcctga cctttgacaa ctttaccgat tctttagttc
      301 tacatagcgc ttgtaccaaa tgtttacata ggcttctgag aaaggaccac gtccattgtt
      361 aatccaatca acaagaattt tgacatgttc ttttaaaata tagtccaagt catcagaata
      421 attcattttg cgtttgtgac gctcgtactc ttcaacgtcc aagagacgtt tttccccatc
      481 tgtaaaaatt ttaacatcca aatcgtaatc aatatacttc agtgcttctt catccagata
      541 gtaggggcta gccatattgc aatagtaaga agttccatta tcacgaatca tggcaatgat
      601 attaaaccaa tatttcttgt gaaagtaaac aatagccggt tctcgagtga cccaacgacg
      661 accatcactt tcggtaacaa gtgtatgatc gttgacacca ataatggcgt tttctgttgt
      721 ttttagtacc atggtgtccc gccaagttcg gtggagactc ccatcatgct tataactttg
      781 aattgtaata aagtcgcctt cttttggaag cttcataact aaccaacttt ctacaattta
      841 taagtttatc atttactatt gtaccataaa attacctaaa atctgtgaat ttcacttgga
      901 aatattaaag atattctcta agagcgcttg ctatatccga aaaatcgtag ccctttcgtg
      961 ctaaaacttg agttaaacgc tgcttcagtt cgtatccttc atactttcga gcatacttag
     1021 tatattgctt atcaagttcc ttgaagatga gttcttgagt cgtttcttgg tcaacttggc
     1081 tatctaagtc gtcaaaggca attttagcat cagaataaga gaagcccttg ttagtcaagt
     1141 tctggataat cttatcttgc agggcacgag ctggaagttt tccctcatat tttttcaata
     1201 gtttattggc tacacgttga gcaacttccg aaaaatcaaa atcattcaag atttcttcta
     1261 tagtagattt tgaaattcct ttttgtgcta atttctgagt cagtacataa ggtcccttgt
     1321 ctcctgaaag ttgattggca ttgatgatag cataagcgta ctgaccatca ttaatccact
     1381 tatcttcttt aagattagca ataacttgag aaacgatgtt tttatcaata tcgtattttt
     1441 tcagatattc tctgacttct ttttcagtgc gtgctttaaa ggataagtgg tagagggcca
     1501 gattcttacc ataagaaaat tgagcaaagt cttgaatctc tttcaattcc tcttcgctta
     1561 tcaccttatc tctcgataac ataaaacgaa caattgtatc ttcggtgata tagcatttgt
     1621 cgccattatc aagctccatc agatagagtc tttttttctt ttcaagtttt gtgattttca
     1681 tagctctatt ataactcaaa atgtgataag ataggggtat gaatctgaaa gtgaaacaaa
     1741 aaataccatt aaaaatcaag cgcatgggaa ttaacggtga gggaatcggc ttttaccaaa
     1801 aaacattagt ctttgtacca ggagctctca aaggcgaaga tatctattgt cagattactt
     1861 ctattagacg caactttgtt gaagcaaaat tactgaaggt caacaagaag tctaaatttc
     1921 gaattgtgcc atcttgtact atttataatg aatgcggagg ctgccaaatc atgcacctgc
     1981 attatgataa gcagctggag ttcaagacgg acttacttca tcaagcgctg aaaaaatttg
     2041 ctcctgcagg atatgaaaat tatgaaattc gtccaactat tggaatgcag gaaccaaaat
     2101 attacagagc taagttacaa tttcagaccc gaaaatttaa aaatcaggtc aaggcgggct
     2161 tatatgcaca aaactctcac tatttagtag agttgaaaga ctgcctggta caagataagg
     2221 aaacccaagt gattgctaat cgcttagcag aattacttac ttatcaccag attccaatca
     2281 cggatgagag aaaagttcta ggtgtccgta ctattatggt ccgacgcgcg agaaagaccg
     2341 gacaggttca gattattatt gttacaaacc gccagcttaa tttaactcaa ttggtaaaag
     2401 agttggttaa agatttccca gaagttgtga cagtagctgt taatacaaat acagctaaaa
     2461 ccagtgagat atatggtgaa aagacagaga ttatctgggg gcaagagagt attcaagaag
     2521 gtgtactcaa ttatgaattt tcactatccc ctcgagcttt ttatcaacta aatcctgaac
     2581 aaacagaagt cctttatagc gaggcagtaa aagcgctgga tgttagtaaa gaagaccatt
     2641 tgattgacgc ttattgtgga gttggaacaa ttggttttgc ctttgcaaag aaagtaaaaa
     2701 cactcagagg tatggatatt attccagaag ctattgaaga tgccaagcga aatgctaaaa
     2761 gaatgggatt tgacaatact cattatgaag ctggaacggc agaagagatt attcctcgtt
     2821 ggtacaagga aggctaccga gcagatgctt tgattgttga cccaccacgt acaggtctgg
     2881 atgataagtt attagatact attcttactt atgtaccaga aaaaatggtt tatatttctt
     2941 gtaatgtttc gaccttggct cgtgatttgg tacgcttagt agaagtctat gatcttcatt
     3001 atattcagtc ggtcgatatg ttcccacata cagctcgaac tgaagctgtt gtaaaattaa
     3061 taaaaaaagt ttaaaaaagg
//
LOCUS       DAVKWS010000105         3078 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKWS010000105 DAVKWS010000000
VERSION     DAVKWS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.106"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            540..896
                     /locus_tag="VW852_002048"
     CDS             540..896
                     /locus_tag="VW852_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273106.1"
                     /translation="MKRDVRDIRKQFRLTDEEEKQILALMREQGDTNFSDFLRKSLLS
                     SDLQKQIEIWFTLWQSQKLEQISRDVHEILISIQSERQVTQEHVSILLTCVQELIKEV
                     GNSIPLSQDFQDKYMR"
     gene            901..1266
                     /locus_tag="VW852_002049"
     CDS             901..1266
                     /locus_tag="VW852_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV0273107.1"
                     /translation="MEHRYRINLKKVFLSDIELNQLNRNIDQSSCQSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISSEELQELKKGIAELIKE
                     VEKEFTIQAQKLREFHGHH"
     gene            1253..3076
                     /locus_tag="VW852_002050"
     CDS             1253..3076
                     /locus_tag="VW852_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV0273108.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPDKTKNLTLVSDYDMRNFLD
                     FPSYEEMVQMYHENFINNDTLYNFRHDRLEEKQRQIHAHHIIQSFSPEDNLTPEQINQ
                     IGYETMKELTSGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQQYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFSHKHATFFMTDSSMKQVVRGNKLNRKQPYTEEFFKNYFAKREMEELMEFLLSK
                     AENIDDLIETAKLFDLTIIPKQKHVYFQLAGVEVKETELDQKNLYGVEFFQDYFESRK
                     DWQNPEIEDLVQFYQEEKFSKEKELPRDEKFWEFYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYIKVKFGIRQEGLIFVPNMQLDIEEEKVKVFIRETSSYYVYHKDDPEKNRY
                     MKGRTLIRQFSSENQTIPFRRKTTVDMIKEKIEEVYTLLELDMDKHSYITIKDDLIHE
                     LAASELRINALQERVTTLNQVAEYLLASVENKQKMKLNLSKLNITDKIGIDVVEKELN
                     ELGIQIELESNRYENIVFKVNKFINRLSKKISKEEGICFQK"
BASE COUNT         1087 a          488 c          609 g          894 t
ORIGIN      
        1 ctaaagtgat attatcaatt ccaaagatga tataacaatc ttcatgatgt gatgtattga
       61 caaaatttaa gatatcacgc accaattcag ttttagaatg atgccatttt tgtttaaaat
      121 catgaaactc atcttcagga gtattcaata gtaaatgtat ttgatctaga ctcagcattt
      181 ttatttctcc aaatattagt ttacagaaat tttatcatag aatcagagaa agtcaatcat
      241 aactatatta gctgtgatat cttttccaaa tcttccctgt tatcctaata tcattcatag
      301 aaaatcaatt tttgattttc tatgggggtt tgggggcgga gccaccaagt tatcttatcg
      361 ttagctgtca aaactggaag gttttagtca gctggcgata tgatttttgg gatattgtgg
      421 acacaatatc tgagctcgca aagccttaca agaatgaaaa tcagttggtg taaaagtgta
      481 ctgacattgt acggtagcag acattgtcag tacaggtatt ttggaaagga agtagtagta
      541 tgaaacgaga cgtgcgtgat attcgaaaac aatttcgcct aacagatgaa gaggaaaagc
      601 aaattttagc tttgatgaga gagcagggag atactaattt ttcagatttt ctccgtaaaa
      661 gtttactctc ctctgattta caaaaacaga tagagatatg gttcaccctt tggcaatctc
      721 aaaaactaga acaaatcagc cgtgatgttc atgagatttt aatttcgatc cagtcagaac
      781 gtcaagtcac ccaggaacat gtatctattc tcttaacctg cgtacaggaa ttgattaaag
      841 aagttggcaa ctccattcct ctcagtcaag acttccaaga caaatatatg aggtagacac
      901 atggaacatc gttaccgaat caatctcaag aaagtgtttt tgtctgatat tgagttgaac
      961 caactaaata gaaatatcga ccaaagtagc tgccaatctt tttctgaata tgcgagacgc
     1021 accctacttg accctggaat gaatttcatc accattgata caaatggcta ccaagactta
     1081 gtgtttgagt tgaagaggat tggcaataac atcaatcaga ttgctcgaag tgtgaaccaa
     1141 tctcagttga tttctagcga agaattgcaa gagttgaaaa agggaatcgc tgaattgata
     1201 aaagaagttg agaaagaatt tacaatacaa gctcaaaagc tgagggagtt ccatggtcat
     1261 cactaagcat tttgccattc atggaaagag ttatcgcaga aagctcatca agtatattct
     1321 caatcctgat aaaacaaaaa atcttacctt agtgtcggat tatgatatga ggaattttct
     1381 ggactttcct agctatgagg aaatggtgca gatgtatcat gaaaatttta tcaacaatga
     1441 cacgctttac aattttcgac acgataggct ggaggagaaa caacgacaaa tccatgctca
     1501 ccatattatt cagtcctttt cgccagagga caatctcact cctgaacaaa tcaatcagat
     1561 tggttatgag accatgaaag aattgactag tggaaagttt cgctttatcg ttgcgaccca
     1621 cgttgataaa gaccatctgc ataatcacat cattataaac tcggttgaca gcaattctga
     1681 caaaaaactt aagtgggatt ataaagtgga gcgaaatctt cgaatgattt cagatagatt
     1741 ttctaaaatt gcaggggcga aaattataga gaatcgctat tctcaccagc agtatgaagt
     1801 ttatcgcaag accaatcaca agtatgaact caagcagcga ctctattttt tgatggaaca
     1861 ttctagagac tttgaggatt tcaaaaagaa tgctccacta ctacatgtag aaatggattt
     1921 tagccacaag catgcgacct tttttatgac ggactcatcc atgaaacagg tggtgcgtgg
     1981 caataaactc aatcgcaagc aaccttatac agaagaattt tttaagaatt actttgccaa
     2041 aagggaaatg gaagaactca tggaatttct cttatcaaag gctgagaata tagatgactt
     2101 gattgaaact gcgaaacttt ttgatctaac tatcattccc aaacaaaagc atgtgtattt
     2161 ccagttggca ggagtggagg tgaaagagac agaactagac cagaaaaatc tttatggtgt
     2221 agaatttttc caagattatt ttgagagtag aaaagattgg caaaatccag aaattgagga
     2281 tttggttcaa ttttatcaag aagaaaaatt ttccaaagaa aaagaactcc caagagatga
     2341 gaagttctgg gaattctatc aagagttcaa gagcaacaga gatgccgttc atgagtttga
     2401 ggtagagttg tcactcaatc aaattgaaaa agtagtagat gatggtattt acatcaaggt
     2461 caagtttggt attcgtcagg aggggcttat ctttgtgccg aatatgcagc tcgatattga
     2521 agaggagaag gtgaaagttt ttattagaga aactagctcc tactatgtct atcataaaga
     2581 cgatcctgag aagaatcgct acatgaaagg tcgaaccttg attagacagt tcagctctga
     2641 aaatcaaacg attccattcc gcagaaaaac gacagtcgat atgattaaag agaagattga
     2701 ggaagtgtat actttacttg agctggatat ggataagcac tcttatatta caattaaaga
     2761 tgatttaatt catgaattgg cagcttcaga attgagaatc aatgctttgc aagaacgagt
     2821 gacaacctta aatcaagtag cagaatattt actggcttca gttgaaaata agcaaaaaat
     2881 gaaattgaat ctttcaaaat tgaatataac tgataaaatt ggcattgatg ttgttgagaa
     2941 ggaattgaat gagttaggta tccagataga attagagagc aatagatatg agaatatcgt
     3001 atttaaagtg aataagttta tcaatcgttt gagtaagaaa atttcaaaag aagaagggat
     3061 atgttttcaa aaatagga
//
LOCUS       DAVKWS010000106         3029 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKWS010000106 DAVKWS010000000
VERSION     DAVKWS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.107"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            54..1079
                     /locus_tag="VW852_002051"
     CDS             54..1079
                     /locus_tag="VW852_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0273109.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHALDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGLSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNILG
                     NACKNAK"
     gene            1069..2076
                     /locus_tag="VW852_002052"
     CDS             1069..2076
                     /locus_tag="VW852_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0273110.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2076..>3029
                     /locus_tag="VW852_002053"
     CDS             2076..>3029
                     /locus_tag="VW852_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0273111.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLL"
BASE COUNT         1033 a          430 c          526 g         1040 t
ORIGIN      
        1 caatgataat tattatcgat acaatagact tgaaatatgt ttaaggagtt tttatgaaaa
       61 acaaattttt tctaatagct attttagcta tgtgtatagt ttttagcgct tgttcttcta
      121 attctgttaa aaatgaagaa aatacttcta aagagcatgc gcttgataaa atagttttag
      181 atcatgcttt cggtcaaact atattagata aaaaacctga aagagttgca actattgctt
      241 ggggaaatca tgatgtagca ttagctttag gaatagttcc tgttggactt tcaaaagcaa
      301 attacggtgt aagtgctgat aaaggagttt taccatggac agaagaaaaa atcaaagaac
      361 taaatggtaa agctaaccta tttgacgatt tggatggact taactttgaa gcaatatcaa
      421 attctaaacc agatgttatc ttagcaggtt attctggtat aactaaagaa gattatgaca
      481 ctctatcaaa aattgctcct gtagcagcat acaaatctaa accttggcaa actttatgga
      541 gagatatgat taaaattgat tcaaaagcct taggtatgga aaaagaaggt gatgagttaa
      601 tcaaaaatac tgaagctcgt atatccaaag aattagaaaa acatccagaa atcaaaggaa
      661 aaatcaaagg aaaaaaagta ttatttacta tgattaatgc tgcagataca tcaaaattct
      721 ggatttatac tagcaaagat ccaagagcaa attatttaac agatttaggt ctagttttcc
      781 ctgaatcatt aaaagaattt gagagtgaag atagttttgc aaaggaaatt tctgcagaag
      841 aagcaaataa gataaatgat gctgatgtaa tcataactta tggtgatgat aaaactcttg
      901 aagctttaca aaaagatcct cttttaggta aaataaatgc aattaaaaat ggtgccgttg
      961 ctgtaattcc agataataca ccgttagcag cctcatgcac tccaacacca ctttcaataa
     1021 actatactat tgaagaatac ctaaatattt taggaaatgc atgcaaaaat gcgaaataaa
     1081 aaacaaataa acctaggcat aatttttgta atctgcctag gtcttcttat tacaatattt
     1141 ttgtcattaa agcttggaac aaaagaaatt aatatcagag attttttagc agcttttgga
     1201 atgggcaata caaatgatga ttttattaaa tcaattatat ataaaagaat acctagaact
     1261 atttttgcaa ttttagcagg ttctagtctt gccataagcg gtgtattgat gcaatcagtt
     1321 actagaaacc caatagctga tccaggtata ctcggtataa acacaggagc aagtcttagt
     1381 gtagtaattg gtctttcttt tttaggaatt tcatcaagca taagccatat aagttttgca
     1441 atcattggtg gcttagtaag tgcaattttt gtatacgcga ttgctgtaag cggaaaagca
     1501 ggccttaccc ctataaaact agccttatca ggaacttgtg ttagtatggc tttaagcagt
     1561 tttgtaagtt ttttaatttt accgaataat aacgtcttag acaaatttag attttggcaa
     1621 ataggtagcc ttggagcagc tacattatct tctatatcta cactactacc ttttataatt
     1681 ttaggtcact tgatagctat atttatttca tcagatttaa acgctttagc tatgggtgat
     1741 gaaatggctg ttggtcttgg agttaatgtt aataggataa gatcacttgc aataattgca
     1801 agtgtgcttt tatgttcaag tattactgca attggtggac ctattggctt cgtaggtctt
     1861 atagttcctc acttttgtgg cttatttata agcaaagata tacgcacaat gaccatttct
     1921 tcatctttta taggtgcaga gctcttgctt atatgtgata taatcggccg tatgttaggt
     1981 aaaccaggtg aaattgaagt agggataatt actgcaataa tcgggggtcc agtacttatt
     2041 tatgtaacta tgaaaaatag aggggttaat aactaatgca aaatttaatt ataggtattc
     2101 aaaaaagaaa aaatagaata acactatttt cctcactatt tttattaata ataatcagtc
     2161 tatcattttt cattttactt atcggagatg aaagttattc tttttcaact ttgattaaag
     2221 tcttaaatag tgaaactgtt cctggagcta gtttttcgat tatggaaatt agattaccaa
     2281 aattattagc aggaattata gctggctggt cttttggatt ggcaggattt atctttcaaa
     2341 ctatgttaag aaatcctctt gcaagtcctg atataatcgg tgtcacaagt tcttcatcta
     2401 ttgcagcggt cttttgcata ttggtattaa aaacaaatag tttaactact ggaattattt
     2461 caataacttg tggactaaca tcatctttaa tattattttt actagctaaa aaagatggtt
     2521 tttcagcagc aagactgata atattaggta ttggttttca agctgtcaca agagcaggca
     2581 cctcattttt attgttgaaa gtagcaagat atgaattaca agaagttatg agatggctca
     2641 gtggctcttt atcttttaca aagttagatg acatacctct tgttctaata gtaagtatta
     2701 ttgctactat attagtttta ttttttaata aaagactaga aattattgaa cttggtgaag
     2761 aaatagcaat cggacttgga gcaaatcccg agctttcaag gcttgtttta attttttgcg
     2821 ctgtatcttt aactgctttt tctacttcaa ttacaggacc aatagcttgt atatcttttt
     2881 tagctggtcc catagcctta aatattggca agaaaagaag cccaatatta gctggattgg
     2941 ttggaatttt actagttttg ttatcagaca tattctctca aaatatttta ccagctagat
     3001 atccagtagg tgttgtaact ggcttgtta
//
LOCUS       DAVKWS010000107         2822 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKWS010000107 DAVKWS010000000
VERSION     DAVKWS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.108"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            105..1451
                     /gene="gdhA"
                     /locus_tag="VW852_002054"
     CDS             105..1451
                     /gene="gdhA"
                     /locus_tag="VW852_002054"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV0273112.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(1957..2127)
                     /locus_tag="VW852_002055"
     CDS             complement(1957..2127)
                     /locus_tag="VW852_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273113.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2140..2718)
                     /locus_tag="VW852_002056"
                     /pseudo
     CDS             complement(2140..2718)
                     /locus_tag="VW852_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2735..2822)
                     /locus_tag="VW852_002057"
                     /pseudo
     CDS             complement(2735..2822)
                     /locus_tag="VW852_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          891 a          537 c          482 g          912 t
ORIGIN      
        1 gaattgaaag aatttttaga aaatttccgt tttttcttta cagtgaaaaa aaattctgct
       61 ataatgtctt atgtaataaa atatgacaat aaaaggagaa cgatatgaca tctgctaaag
      121 aatatatcca aagcgtgttt gaaactgtga aagctcgtaa cgggcacgag gctgaattcc
      181 tccaagctgt tgaagaattt ttcaacactt tagaacctgt atttgaaaaa caccctgagt
      241 atatcgaaga aaatatcttg gcacgtatta ctgagcctga gcgcgtggtt tctttccgtg
      301 ttccttgggt tgaccgtgat ggaaaagttc aagtaaaccg cggttaccgt gttcaattca
      361 actcagctgt tggaccatac aaaggcggac ttcgtttcca cccaactgta aaccaaggga
      421 tcttgaaatt cctcggattt gaacaaatct ttaaaaatgt cttgactgga cttcctatcg
      481 gtggaggtaa aggtggatca gacttcgatc ctaaaggtaa aacagatgct gaagtgatgc
      541 gcttctgcca aagcttcatg actgaattgc aaaaacacat cggaccatca cttgacgtac
      601 ctgctggtga tatcggtgtt ggtggacgtg aaattggtta cctttacggt caatacaaac
      661 gtcttaacca atttgatgct ggtgtcttga ctggtaaacc tcttggattt ggtggtagct
      721 tgattcgtcc agaagcaact ggttacggtt tggtttacta tactgaagaa atgctcaaag
      781 ctaacggtaa cagctttgct ggtaagaaag tcgttatttc aggttctggt aacgttgctc
      841 aatatgctct tcaaaaagca actgaactcg gtgcaactgt tatttctgtt tctgactcaa
      901 atggttatgt catcgatgaa aatggtatcg acttcgatct tttggttgat gttaaagaaa
      961 aacgtcgtgc tcgtttgact gagtatgcag ctgaaaaagc aactgcaacc taccacgaag
     1021 gtactgtatg gacttacgct ggaaactatg acattgctct tccatgtgcc actcaaaacg
     1081 aaatcaacgg tgaagcagct aaacgtttgg ttgctcaagg cgttatctgt gtatctgaag
     1141 gtgccaacat gccaagcgac cttgatgcca tcaaagttta caaagaaaat ggtatcttct
     1201 acggacctgc aaaagctgcc aacgctggtg gtgtagccgt ttcagctctt gaaatgagcc
     1261 aaaacagtct tcgcctctca tggactcgtg aagaagttga tggccgtctc aaagacatca
     1321 tgacaaacat cttcaacaca gctaaaacga cttcagaaac atacggtctt gataaagact
     1381 accttgcagg agctaacatt gctgcctttg aaaatgtagc aaatgccatg attgcacaag
     1441 gtattgttta agatttattt actcaatcct caatcactac gattggggat ttttagcttg
     1501 aattaaaaaa ttggaaaatc taattaaata aaatgaaatg caataaaaac tgaacaaatt
     1561 gattgcggaa ttcaaaccaa tttctaacat atttctagaa attaatttga cttttctaat
     1621 cgatttgttc atatcttatt tcaatttact atacttactt aacccttttt atactcaatg
     1681 aaaatcaaag agcaaactag gaagcgagcc gcaggctgta cttgattacg gcaaggcaaa
     1741 gctgacgtgg tttgaagaga ttttcaaaga gtattaaaca aaaagaaaaa ccgctactcc
     1801 taagaatagc gatttaatca tgtttttaag ctactactgt agttgctcta ttatttaagt
     1861 gttatgaata acttcaatat ctttaaagct ttttgttaga tttataattt caactatagc
     1921 catctctact ccttagttta cagcaaaaaa ttatgcttaa ttcttattgt ttttgtaaac
     1981 aatcagcgaa cccaatacta ttccattcaa caagcctaaa gaaataccat attcaactgg
     2041 aaaatctaaa aaaattgcaa acactattcc aatcatcacc cctagtgagg ctgttgttaa
     2101 aattacatat tcattttctt tcttcatttt ctttacacct catttccttt ttgtatataa
     2161 gctgtagtac tctccaccaa gatccattaa gtacttatga tttcctgact caacaatctt
     2221 accacctttc attacaaaaa taacatccgc atccttaatc gttgacaatc tatgagctac
     2281 aattatttga gtacagccct gactttgtat atactttgtt attctttcct cattaatagt
     2341 gtctaatgca ctagttgctt catctaaaat tacaatacta ggattattta ttaatgcacg
     2401 tgccagtgct atccgttgcc tttgcccacc tgaaatattt gaccccatct ctgagatgat
     2461 agtattaaat ttcatcggca tagccatgat ttcatcatag atttgaactg ctttacaaac
     2521 ttcctctatc ttttgtgaag taacttcgtg ctttaaagtt atattatcaa gaatacttcg
     2581 gttcaataaa aatgaatcct gtggaactac tcctaaattt tgacttaata tttttttatc
     2641 tatttgatta atatttacac catcaaataa aacttttcct gtatcaatct tatataatct
     2701 tgatggtatt tttgataata gacttcctgc aaaactagaa tcctagttca tgattgataa
     2761 taccagcaat caaattcatt cgtaatccga agcgtttaga tgatttcgat aggttgttga
     2821 aa
//
LOCUS       DAVKWS010000108         2806 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKWS010000108 DAVKWS010000000
VERSION     DAVKWS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.109"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..846
                     /locus_tag="VW852_002058"
     CDS             <1..846
                     /locus_tag="VW852_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0273114.1"
                     /translation="IVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQG
                     KLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEI
                     PESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHS
                     YQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHF
                     IYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAH
                     IDALI"
     gene            903..1700
                     /gene="psrA"
                     /locus_tag="VW852_002059"
     CDS             903..1700
                     /gene="psrA"
                     /locus_tag="VW852_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV0273115.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLTFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1711..2319
                     /locus_tag="VW852_002060"
     CDS             1711..2319
                     /locus_tag="VW852_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0273116.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(2267..>2806)
                     /locus_tag="VW852_002061"
     CDS             complement(2267..>2806)
                     /locus_tag="VW852_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0273117.1"
                     /translation="WVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLEFS
                     LLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIFQL
                     TPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQ
                     ELITQKVEKLFEKVNQLWK"
BASE COUNT         1011 a          413 c          471 g          911 t
ORIGIN      
        1 atagtagaag caatcgaatc agctttagaa aaagtagatg aatatgctga aagttataat
       61 agactagaac agctagataa agaatttcca gataaactaa aaaaatctat tcttcaatat
      121 gctatgcaag gaaaattagt tgaacaagac ccaaatgatg aatcagtcga agttttactt
      181 gaaaaaatac gagcagaaaa acaaaaactc tttgaagaag gcaagattaa aaagaaagat
      241 ttggacattt ctattgtttc ccaaggagat gataactctt attatgagga agtaccttgt
      301 gaaataccag aaagttggga atgggtgagg ttaaacgata taacctccta cattcaaaga
      361 gggaagtctc caaaatattc aaatattcct atataccctg tgattgctca gaaatgtaat
      421 caatggtctg gcttttctat tgacttagca agatttattg atcccgaaac agttcatagt
      481 taccagaaag aaagattact aagggatggt gatttaatgt ggaattcaac aggtctaggt
      541 acgttaggac gattagctat ttaccatgaa aataaaaatc cttatgcctg ggctgttgca
      601 gatagccatg ttacagttat aagagtctta tcgggggtta ttaattgcca tttcatctat
      661 aattttcttt cctctcctat tgttcagtca gttattgaag aaaaagcgtc gggtagtaca
      721 aaacaaaaag aattattaac gaaaactata aaagaatatt taatccccct cccacccctt
      781 cctgaacaat ccagaatcgt tgataaaatc gaacaatttt tcgcccatat tgacgcacta
      841 atttagtata atagttttac gtgcggtgga atttctatca aacataagat aggagtttta
      901 cgttgaaaga ttttgaatta catttgaaga aagctggttt agctgaaaat actgtgcgtt
      961 cgtaccttta tggtgttcga tttttcttgg agaattacga actcaaaatg gaagatttat
     1021 ttgaatacaa aggatattta ttagacaatt ttaaacctaa aactgtcaat ttgcgactac
     1081 aaggagtaaa taaatatctg acttttattg gtcatgatga tttgaaatta aaatttgtca
     1141 aagtacagca aaaaccgttt ttagaagatg tcattagcca tgctgattac ctttttctta
     1201 aacgtagttt gaaaaaagat ggtattttaa aatggcattt tgtcgtttgg tttttaggtg
     1261 caactggggc tcgtgtaagt gaacttatta aactaaaagt agagcatgta gaaatcggtt
     1321 attttgacat ttactcaaaa ggtggtaaaa ttcgtagact gtacattcct aaaaaattaa
     1381 gaaatagttg tcttagttgg ttagaatcag aaaatcgtcg aagtggttat ttatttttga
     1441 ataaatttaa cgaaccaatt acagcaagag gagttgctca acagttaaaa aattatgctg
     1501 ataaatacaa aatgaatcct aaagtaattt accctcattc ttttaggcat ttatttgcta
     1561 agaatttttt agcgaagtat aatgatattg ccttgcttgc agatttgatg ggacacgaaa
     1621 gtatagaaac tactcgaatt tatctaagga aaacagctac tgaacaacaa aatattgtag
     1681 ataaaattgt taattggtaa aaaataacag gtggtcaaac tgactacctg ctatttttgt
     1741 gattatggct cttattatgg gaataaagac gaaacaacct cttatcctat atatgaaatc
     1801 ccagaagcat ggagatatat taaatttgct agcctagtta acttcagaat aggaaaaact
     1861 cctccacgca gtgaagctac tttttgggga actgaaatac cttgggtatc tatatcagat
     1921 atgcctatat ctggttatgt aactaatacg agagaatcta tttctaaatt agcattaaaa
     1981 tcaaagaaaa tagacatttc acctaaagga actttattaa tgagttttaa attatctatt
     2041 ggaaaagttg ccatattgga tattcctgcc actcataatg aagccattat atcgatattc
     2101 ccctatgcta ataaagaaaa tattatcaga gattacttaa tgatattttt gccacttatc
     2161 tctactttag gtgattcaaa agatgctatc aaagggaaaa cattaaatag taccagtatc
     2221 tccgaattat taatccctat ttctaatcat gaagagatga aaagaatcat ttccaaagtt
     2281 gatttacttt ttcaaaaagt ttctcaactt tttgagtaat aagttcctgt tcctcaaaag
     2341 gagctaacgg aattaatagc tcgctcagtg tagttttagg aatattatat aaagcttgac
     2401 ctgatagttt agttattgct ttcaattgtt tataaaataa cggagaggac aagttaaata
     2461 atagaaattt tgaaataatc tctgaacttt cgaatggtgt taattggaaa ataaatccac
     2521 cagccacaac accatcatag tctttatcga ttcttgcaaa ctttccaata tgttctaaag
     2581 aggttgatac aggtgttatt agctgattat gttttaaata aacttgctca gaggagatga
     2641 attgtgtatc aatgtagtaa tcgttatcca acagagaaaa ttctaaaggc ttaatattac
     2701 caccacgtat aattctaaca cctttattat taatgcttaa atcgcccttc ttgtaagaaa
     2761 gacctgtatt cattgaaaaa atatctttta tttttataac aaccca
//
LOCUS       DAVKWS010000109         2744 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKWS010000109 DAVKWS010000000
VERSION     DAVKWS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.110"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..304)
                     /locus_tag="VW852_002062"
     CDS             complement(<1..304)
                     /locus_tag="VW852_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273118.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKERDYIGRRDSQVDNLRVIGNIFLNYLTNRKYSLNMNRNGCMGDFPHDF"
     gene            complement(362..1075)
                     /locus_tag="VW852_002063"
     CDS             complement(362..1075)
                     /locus_tag="VW852_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV0273119.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1472..1702)
                     /locus_tag="VW852_002064"
     CDS             complement(1472..1702)
                     /locus_tag="VW852_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEV0273120.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            complement(2124..>2744)
                     /gene="phtB"
                     /locus_tag="VW852_002065"
     CDS             complement(2124..>2744)
                     /gene="phtB"
                     /locus_tag="VW852_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV0273121.1"
                     /translation="LDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPK
                     GYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQT
                     EKPEEETPREEKPQSEKPESPKPTDEPEEESPEESEEPQVETEKVEEKLREAEDLLGK
                     IQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          831 a          541 c          448 g          924 t
ORIGIN      
        1 aaaagtcatg aggaaaatct cccatacagc cattacgatt catattgagg ctatattttc
       61 gattagtaag ataattcaga aatatatttc ctattactct aagattatct acttgtgaat
      121 cccttctccc aatataatcc ctttctttga ctaatgatgg agatttccaa gtaaaaatta
      181 aatcgccaga gaaatttttc ttatcaaaaa acttaaaaag agattgataa atactatttg
      241 ctaattgaca agtgtcagat gtaaattcat aattaacagc ttcattaaaa tcaacttcag
      301 acatttcaat gtactcttca atagtaatat tagctttttt gtagtcatta gctcgtctcc
      361 tttaaaatgt gatcttttca agaatatttt tatctaaaca atccagcaag tcttggtaag
      421 aatagacttc gtaagtcggc tgggcttgtg tgtgattttc gaggtgatga ggattatacc
      481 agatagtgtc aatccccgca ttattgccac cttgaatgtc ggcagttaga gaatctccaa
      541 tcatcagcgt cttttcttta ctaaatccag caatttgctg gccaatcttt tcataaaaga
      601 gagcatccgg cttttgagtt tgcaactgtt ctgagataaa gacttgattg aaataaggtg
      661 ctagaccaga ttgagccaaa cgtcctgtct gaatggcagt aatgccattt gtcgcagcat
      721 acaagttata atcacgctca atgaggctgt ccaagagatc atgagcgccc gatagtgttt
      781 gtccctgctg ggcgaggtaa aattggtaac gctgggcaag aaaactaccg tctttttcct
      841 gtccaaaatg agcaaataaa cgagaaaatc gcgtgttaac cagctcttgt ttactgattt
      901 tcttcagctc caagtctttc cagagagcct tgttcatagg aacgtaataa tctttataag
      961 cctgaatatc cgcaactcct tcttctttta gaagttgggt caaagccaca tcctcagcag
     1021 catcaaaatc aagaagagtg tggtcgaggt cgaagagtag aaatttgtag aacaatttga
     1081 ggttttcctt tctgaaaatt cattaagaac attatatcat aaagcacctc atacaattaa
     1141 ctaatttaat cacttaaaaa aaattcgaac actttctata caactgacag ctcaaatctt
     1201 tcagaataga acaatactaa ctatcgaaca ccccgtcttc ataaatacat atgtaattct
     1261 aggcctagaa ttcctataaa ctaaatgctt tcatactctt ccaagtaatt gattgcctta
     1321 aattttaatt tttgaaggtt tctaaagcta gaatagcccc atcacaatca gttttgattg
     1381 attcacaatt tagaaacact atagtttcac tcctgttaaa ataaaaagga actgcataaa
     1441 gcaatccctt tctgattttg aaatcattta cttaacattt tatagcagaa cctaggtcaa
     1501 aaaaggcaag taaagtatct gctggatttt cggaaacaac cctatctact tgatcaaaac
     1561 tcgttccaat tcctccgccc tcggttcctc ctacataagt aatcggaaca tctttagcta
     1621 ctttactaat cagttcaaca acaccttctg caatgtgttt ggaatgtgaa acgataacaa
     1681 gaccaatacc aatactttcc atcaaaccac cccagtttct aaaatagcag taaagagtaa
     1741 tcctgatgag aatgatccag gatcaatatg tccaagaaac cacatgctcc taagacaaga
     1801 gctaacagac tggccattaa taatagtatt gttctttttt tcatcattac tccttaacta
     1861 gtgtttaact gattaattag ccagtaaata gtttatcttt atttacacta tctgttaaga
     1921 tatagtaaaa tgaaataaga acaggacagt caaatcgatt tctaacaatg ttttagaagt
     1981 agaggtatac tattctagtt tcaatctact atattttgca cattttcatg aaaaaaatga
     2041 gaactagaac tcacattctg ctctcatttt tcgttttccc gttctcctat cctgttttta
     2101 ggagttagaa aatgctgcta cctttactta ctctccttta ataaagccaa tagtttttca
     2161 gcttctgcca taatagtatt gttgtcctgg gtgccaaata gtaaattatt ttttaatcct
     2221 gtgagagtct ctttggcatt ggacttgata attggatcct ggatttttcc aagtaaatct
     2281 tcagcctctc tcagtttttc ttcaaccttt tcagtctcga cctgaggttc ttctgattcc
     2341 tctggtgatt cttcttctgg ttcgtctgtt ggttttggag actctggttt ctcgctttgc
     2401 ggtttctctt ctcgaggggt ttcttcctca ggtttttctg tctgaggttt ctcctcgttt
     2461 ggtttttccg tttgattggt atcagcttga ccatttttgt ttctttgaac atggtcgcta
     2521 gcgttaccaa aaccattatc tgaatgcgga cgttcgtttg gatgttcgac atagtacttg
     2581 acagtcgcca aaagatcctc aagagtatac cccttaggtg cctcataaag gccttcgtca
     2641 aaccactcaa atttgatgtt atggtaatgg tcataatgag gtatgattaa actaccgttt
     2701 ttgacttcta cagtatattg aagattgtaa ggcatgcgat caag
//
LOCUS       DAVKWS010000110         2717 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKWS010000110 DAVKWS010000000
VERSION     DAVKWS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.111"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            52..579
                     /locus_tag="VW852_002066"
     CDS             52..579
                     /locus_tag="VW852_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0273122.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            656..1012
                     /gene="glnR"
                     /locus_tag="VW852_002067"
     CDS             656..1012
                     /gene="glnR"
                     /locus_tag="VW852_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV0273123.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            1049..2395
                     /gene="glnA"
                     /locus_tag="VW852_002068"
     CDS             1049..2395
                     /gene="glnA"
                     /locus_tag="VW852_002068"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV0273124.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2491..2610)
                     /locus_tag="VW852_002069"
     CDS             complement(2491..2610)
                     /locus_tag="VW852_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV0273125.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT          824 a          485 c          576 g          832 t
ORIGIN      
        1 actcataaaa atcaccgatt tatgataaaa tggaaataga aagttgagat tatgagttat
       61 tttaaaaaat ataaattcga taaatcccag ttcaaacttg gtatgcgaac ctttaaaaca
      121 ggtattgctg tttttctagt tctcttgatt tttggctttt ttggctggaa aggtcttcaa
      181 attggtgctt tgacagccgt ttttagcctg agggagagtt ttgatgagag tgttcatttt
      241 gggacttcgc gtattctagg aaatagtatc ggtggactct atgccttggt cttcttctta
      301 ttaaatacct ttttccacga agccttttgg gtgaccttgg tagttgttcc aatctgcacc
      361 atgttaacca ttatgacaaa tgtagccatg aataacaaag caggggttat tggtggtgta
      421 gcagctatgt taatcattac cctatcaatt ccaagtggtg agacaatttc gtacgtgttt
      481 gtgcgtgtat cagaaacgtt tatgggagtt tttgtcgcaa ttatcgtaaa ttacgatatt
      541 gatcgtattc gtctcttttt agagaaaaaa gaaaaataat gttacatttt ataacattat
      601 caattgacgt ttgtcttttt ttagactata atagacagaa agaaggaaat tgtaaatgaa
      661 ggaaaaagaa tttcgccgaa atatggctgt ttttcctatt ggcagtgtta tgaagttgac
      721 cgatctatcg gcgcgtcaga ttcgttatta tgaagatcaa gagttgatca agcccgatcg
      781 aaacgaagga aatcgtcgca tgtattcctt gaatgacatg gatcgtctgc ttgaaatcaa
      841 agattatatc tctgaaggtt ataatatcgc tgccattaag aaaaaatatg ctgaacgtga
      901 agcgaaatcc aagaaagcgg ttagtcagac tgaagtacgt cgtgcacttc acaatgaact
      961 cctccaacag gggcgttttg cttcagtaca gtcacctttt ggtcgcggtt aggcaaccgc
     1021 aagtagtcat agattaagga gaaaacttat gccaatcaca gctgcagata ttcgtcgtga
     1081 agtcaaggaa aaaaatgtta cctttattcg tcttatgttc tcagatattt tgggaaccat
     1141 gaaaaacgtc gaaattcctg ctacagatga acagttagat aaggtcttgt cgaacaaggt
     1201 tatgtttgat ggatcttcta ttgaaggttt tgtacgtatc aatgagtcgg atatgtactt
     1261 gtacccggac ttggatacat ggacagtctt cccttgggga gatgaaaatg gaagtgttgc
     1321 aggtctgatc tgtgatgttt atacaacaga aggtgaacca tttgcgggtg accctcgtgg
     1381 taatttgaaa cgagctcttc gtcacatgga agaagttgga ttcaaatcct tcaaccttgg
     1441 tccagagcca gaattcttcc tatttaagtt ggatgaaaat ggggacccaa cacttgaagt
     1501 gaatgacaag ggtggctact ttgatttggc acctactgac cttgcggaca acacacgtcg
     1561 tgagattgtg aatgtcttga ccaaaatggg atttgaagta gaagcgagtc accacgaggt
     1621 tgcggttgga cagcatgaga ttgactttaa gtacgatgaa gttctccgtg cttgtgataa
     1681 gattcaaatc tttaaacttg ttgttaaaac cattgctcgc aaacacggac tttacgcaac
     1741 atttatggcg aagccaaaat ttggtattgc tggctcaggt atgcactgta atatgtcctt
     1801 gtttgatgca gaaggaaata acgccttttt tgatccaaat gatccaaaag gaatgcagtt
     1861 gtcagaaaca gcttaccatt tcctaggcgg tttgatcaag catgcttaca actatactgc
     1921 catcatgaac ccaacagtta actcatacaa acgtttggtt ccaggttatg aagcgcctgt
     1981 ttacattgct tgggctggtc gtaaccgttc gccacttgtg cgcgtacctg cttcacgtgg
     2041 tatgggaact cgtcttgagt tgcgttcagt ggatccaatg gcgaaccctt acgttgctat
     2101 ggctgttctt ttggaagttg gtttgtatgg tattgaaaat aaaatcgaag caccagctcc
     2161 tatcgaagaa aatatctaca tcatgacagc agaagagcgc aaggaagctg gtattacaga
     2221 ccttccatca actcttcaca acgctttgaa agctttgaca gaagatgaag tggttaaagc
     2281 tgctctcgga gatcacatct acactagctt ccttgaagcc aaacgaatcg aatgggcaag
     2341 ttatgcaacc ttcgtttcac aatgggaaat tgataattat ttagaccttt actaatacta
     2401 atatagaaga aagattgcct gtgggtaaac agtcacacag cagtctaagc catcaaatac
     2461 ctataaccct taaaatagcg atttcggtcg ctatttattt ttcgaaaaat cctcttgact
     2521 cttttgcgaa ttagatttat catcagacgt gtagaagaaa atcaaggagt cactatggaa
     2581 aaattcaatt tcaaaaacaa tatcgggcaa gaaaacaagt tacttcaaat cgaaatctac
     2641 aagtttacta acttttacaa gcttcaaaat tacacatctg taaatatttt ttcgaaagat
     2701 atatttgaag ccatagt
//
LOCUS       DAVKWS010000111         2665 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKWS010000111 DAVKWS010000000
VERSION     DAVKWS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.112"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..1014)
                     /locus_tag="VW852_002070"
     CDS             complement(<1..1014)
                     /locus_tag="VW852_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0273126.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYG"
     gene            complement(1024..1973)
                     /locus_tag="VW852_002071"
                     /pseudo
     CDS             complement(1024..1973)
                     /locus_tag="VW852_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(1966..2665)
                     /locus_tag="VW852_002072"
                     /pseudo
     CDS             complement(1966..2665)
                     /locus_tag="VW852_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          907 a          498 c          287 g          973 t
ORIGIN      
        1 tccatactta tagcaaatat tttttgcttc atataacgga aaatctctat tcacctcatt
       61 tggttcgata tcattcattt tatttgactc aattggatta attgaataca attttaaaaa
      121 aataggcttc gtaccaataa tagaggataa ttgacctcct aattcaccta gcgctgtaaa
      181 aataacacct gttagtgctc ctattgcttc aatagtacca attttcacta ttccttttat
      241 tgcaagatag cctgttaaaa aaacgagaga tatctgaaaa aaaatattga gaaagaagct
      301 aatagcgcct gctaacgttt ctacagttgt ctttctttgt ataaccatct ttaataaaat
      361 tcctgcttct ttaattttct taggcaatac atataaaaga ttcaaggacg ctaacacatc
      421 aaatccattc aatatagtct cactagattt taaaaaagct tcattttggt tagttaaatt
      481 tagactaact tctcgcattt tcgatgcaaa gatttttggt acaagtagca taatcattaa
      541 tgaaaacaag gtggctacag tcaatgacca atgatagtga ttaagagtca caactgcaaa
      601 tatagtacca gaaattcctt ttattactaa aaaaagttgt ttaaaagcct gatcatttaa
      661 agtctgaacg tcattattta gccacgaaag atatgttcct gatgatttac tatgaaattc
      721 ttgataggta gagttagaga tgtctgtggc aactctattt cgaatctcta gattaaactc
      781 ttggatcact tcaacctgat aatttttcac tacccagtca aggaatatta tcccacacca
      841 gacaatcatt tggtagattg acaatttcaa aaaccgctct aaattcatcg caattaattc
      901 attcaacacc agagcattaa tagttgctgc ataaattagc aataattgac cagcaacaat
      961 aaatatcgtt aataaactaa atttttttat atttgatttt ataatagtat acacaatagt
     1021 ttctcacttt ctaaatttta attgaacata gttgtcatat atacaataga aaaaaccaaa
     1081 atgatataat aacatatatt tcaaaaaaat tcgttaaaaa ttttttcttc tcttgccttc
     1141 ttgattactt ttaaagcctt gcatttgtct cctattaata gtaaccgctt tatgtttaaa
     1201 gaataatatt tctttgtaac caatattctc tcgttgaaac tcaataaatt aaaatatttc
     1261 ctacagtaat tataatattc ttcatctgca ttaattgttt tttgtgtcac tccagtgata
     1321 ccgttttctt tactgtgagc gtagtaattc accaagaatt ctcgcactat atcaatttgg
     1381 tatccttgag caagtagttt taataaaaca acaccgtcct gatgtgaatc tattttctca
     1441 aaaccattaa ttaattctag cacctctttt ttacacaacc aaaatgacgt acctgctata
     1501 ttgtgaacca tttgaacaaa caagggattt ccaacaaaat cggtcttctc ctcttctcgt
     1561 gtaccatttg gataaattat tattccataa ctataaacta aagctaaatt cttcattcta
     1621 ctctttttaa aacaagccat caactttaaa attcgatctg gcatatattc atcatcatcg
     1681 tctaaaaatg atatatactt acctctagaa tttttgatac ctatgtttct ggcattagtt
     1741 gcacctaaat cttcattact taaaattaac ttaattctat gattggtata gccaaattga
     1801 tggataattt tatttcttaa atttacatta ctataattat catcaataat tataacttcg
     1861 atatttttat aactttgatg taaacaactt ttcacagctc taatcagaga ttcataccta
     1921 ttatgtgttg gtattataat acttactaat tcttgatcta tattcctatc catgactact
     1981 cttctctaac aattcatcat atactctcat ggtttctaca aacatttttt gcacagaaaa
     2041 atgttttctt atttttgatt tactattctc acctatatat ttcaaatact cagaatcatt
     2101 gagtaaaaaa ttagcacaag cacacactcc ctcaacatct tccttctcaa ataaaaatcc
     2161 atcaacccta tgttcaataa tttcacttaa cccgccaaca ttactagcta aaaccggagt
     2221 tccttgtgac attgactcta aaacacacat aggtattcct tctgtatcag aaggaatata
     2281 caataaatcc gatatttggt aaactatagt agctggatag atttcaccaa gtaacctgaa
     2341 attatctcta catttcaaat ggcaaatttt ttctttcaaa gcagcccaca tactaccatt
     2401 tccagccata ataaaaataa catcttctct gactaaaaat aatttttctg caaattcaag
     2461 gaatctatcc ggcctttttt ctggatccaa ccttccaaca taacaaatga tttttttgtt
     2521 atttggaata caaaattctt ttttaaagtc ttgaacacct actacatcta aatcgctatt
     2581 tgatacatta attccgttat ttattgcaac tatcttctta tttttttatt atactctcca
     2641 atcttttttt catagtttca gatac
//
LOCUS       DAVKWS010000112         2611 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKWS010000112 DAVKWS010000000
VERSION     DAVKWS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.113"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            207..1265
                     /locus_tag="VW852_002073"
     CDS             207..1265
                     /locus_tag="VW852_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273127.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQ
                     SYLGSNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSKCMPSQYDR
                     FFYKDRPYIFSRKSSIKG"
     gene            1580..2557
                     /locus_tag="VW852_002074"
     CDS             1580..2557
                     /locus_tag="VW852_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273128.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYIGIKHVSIRR"
BASE COUNT          873 a          344 c          437 g          957 t
ORIGIN      
        1 tattatcaag agtgtataga aaatattttt atagagacga tgatgactaa agtttttatc
       61 aataatttgg gctccttgtc aactgtagtg ggttgaagaa aagctaacgt ctggggaagg
      121 ggttaaagct ttgctttatc aaatattgac ttacagagtt gctctattgt attgatgcat
      181 tcttagtaaa taatatggtg ttttaaatga aagatgatca gaaatattta ttagcgggac
      241 tttattcgct tctagttgca atattttatt ttccattgat tgattccaag gggatttttg
      301 taagtatatt gatggcggtt ttacttttat atttaatata cttcattgca acagtcattc
      361 atattgtgat aattaagttt ataagaaaaa aatcttttaa gtatttggtt ctatatccat
      421 ttacttatga tgggagctgg agatttcaac ctataaactt actctacttt ccagaaatgg
      481 tgagagatgt cattccgatt aatttagttc aagaatattg ccaaggacaa ccttacggac
      541 tgttaaaaaa gatgctgaaa agaattcgat tgtcacgaga gatagcttta ctcctggcta
      601 ccattatcgt ctattttttt acgcatagaa ttctacctct ttcagtattt acgttcatgt
      661 tctcttacat attgctattt gtacaatctt atttgggtag caataccgct tggattggaa
      721 atagaagatt aataatagat gatgaatttg aaaaaatctt gctttcaaaa agttatgtaa
      781 aagaaatatc atctgcacga tactcagaat acttgacatg tgaatacaaa aaccttacac
      841 caatcatatt gatagctatt tttgaaaatt tgcttgactc ctacttgctt cagaatcaga
      901 gtgaggttga cttagatatt ttttataaag ttttaccttt gctttataaa gaaaaatata
      961 ctatgggatt caattacttt gtaagtttaa attatttact atataaggtt ggatttttgg
     1021 gaatcattta cgataatgaa gcattaagag atttgtcaaa acaatattta aacaaaaata
     1081 tttcggaatt acaagatggt tctttcgagg gcggtataca agatgctgtt gcctctaaac
     1141 aaatagtagt tataaatgaa tttatagctt gtttaaattc caaatgcatg ccatcccaat
     1201 atgatagatt tttttataaa gatagacctt atattttttc cagaaagagt tctattaagg
     1261 ggtgaaaatt tgttatattt ggattaacaa gtgtcctagg gattagaaat aactctagta
     1321 tagctagata tttttgagta actagaaata tcattaggct actcatatga ttgccagaaa
     1381 agatggctga tgaatcttat ttatactgtt gcagtaggca ttgtctagga taaataagct
     1441 atcttgatgg gggaaattgg aataatttta gtagaaacat taaaggatta tggacaagta
     1501 gcgcaggatt aggtgatgca atgtgctaag ttggcttggt gttagcagta gtattattta
     1561 acaatggaaa gtagactaaa tgaaaaataa gcgttatttt tttgacacaa ttttaatgat
     1621 tttactgctt ataagcacga tattttgtgt cagcccagta tttataaaat tggacatctt
     1681 aggaaccccc tctcatgcca tcttaacctt tgtattggcg ataccgttat tctatatttt
     1741 gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta aattaagacc
     1801 aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact atcgaattct
     1861 ctttcaccaa ctaatgggat ttagtccagg aatagcagtg ttttataagg agagtcaaac
     1921 aactaagaat ctgtttaaat tttactactt cttgtacttt acaacactaa tttcatacta
     1981 ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattt cattttctat
     2041 tatcatagca ttagtacaaa aactttatag aattgagaat caacaattat ttctgttaaa
     2101 atcaaaggta ctaacaatac tggaatctaa gaagaattgt gagtttaatc ttcaggatta
     2161 tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt tatcttatat
     2221 cagtttaatc aagccatatc tttcagagag tgtgcgtgaa caaattgatt tattagaagt
     2281 taagcgattt aaaaaaataa atcatccaat atcactttat ggtatgttgg atgttataaa
     2341 attaaatcta tatttacggc attataatga aaaaaataaa tatgaatcaa tgttaaagaa
     2401 aattttagag gtaagaccag actttgtttt aatcgagcag aatattgatg atagtttaaa
     2461 tagctcgcaa ccattatctt tatctttagc catttcagaa atccaacttt tgcttgaagt
     2521 gtatataggg atcaaacatg tttcaattag gagataaaac cagaataata tttgccatta
     2581 taggggcaag ttgcatatta gtcagtatgt a
//
LOCUS       DAVKWS010000113         2432 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKWS010000113 DAVKWS010000000
VERSION     DAVKWS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.114"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            106..1113
                     /locus_tag="VW852_002075"
     CDS             106..1113
                     /locus_tag="VW852_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0273129.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKIVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1116..1316
                     /locus_tag="VW852_002076"
     CDS             1116..1316
                     /locus_tag="VW852_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0273130.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1468..1650
                     /gene="rpmF"
                     /locus_tag="VW852_002077"
     CDS             1468..1650
                     /gene="rpmF"
                     /locus_tag="VW852_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV0273131.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1666..1815
                     /gene="rpmG"
                     /locus_tag="VW852_002078"
     CDS             1666..1815
                     /gene="rpmG"
                     /locus_tag="VW852_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0273132.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2295..>2432)
                     /locus_tag="VW852_002079"
     CDS             complement(2295..>2432)
                     /locus_tag="VW852_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273133.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT          805 a          467 c          446 g          714 t
ORIGIN      
        1 gaaattattg ggctgaaagt ttttagtgat tatttttcaa ctttctattg actttataaa
       61 ttaaagtgat atcatttaga tatcaaataa caggaggtca ttcttatgac tgaaaaacta
      121 atcaattcaa aaccaaatgg tgtattcgca ttgattctca ttgagttgac aatcgtactt
      181 ggtatcttta tatttataat gggggttggt tcggaaaaca tttttggaat tattatcgga
      241 cctttactaa tcgtaattgc aggtctagct catgctggtt taaaaattgt caaacctcaa
      301 gaagctctgg ttctgacact ctttggtaac tatacaggta ccatcaaaga acctggcttt
      361 tactttgtca atcccttcag cgtagcagtc aaccctgcaa accacactcg acttggacaa
      421 agtggtgatg ttagcacaaa atctcctttt ttaggagcta aatcatcaaa tgacaatgat
      481 gtaaatcttg aaattggcaa gaaacagatt tccctcaaag tcatgacctt gagcaattct
      541 cgtcaaaaaa tcaatgattg cttaggaaac cctgtagaaa tcggtatcgc ggtaacttgg
      601 agagttgtcg ataccgctaa ggcagtcttc aacgttgata actacaaaga atatctttca
      661 ctgcaatgtg atagcgccct ccgtaatatt gtccgcatct atccttacga tgtgtctcct
      721 aatgtggata ctacgggtga cgggcaagca gatgaaggta gtctccgtgg ctctagcgaa
      781 attgttgcta accgtattcg tgaagaaatc caaagtcgtg ttgaggatgc tggcttggaa
      841 atccttgaag cacgtatcac ttacctagct tatgctccag aaattgctgc cgttatgctt
      901 caacgccaac aagcatctgc cattattgat gcacgtaaga tgattgtaga tggtgctgta
      961 ggaatggttg aaatggcact agaacggctc aatgaagggg aattggtaga acttgacgaa
     1021 gaacgaaaag ctgccatggt ttcaaatctc cttgtcgttc tttgtggcaa tcatgatgca
     1081 caaccaattg tcaacacagg aagtctttac taaagatggc tgaagctaaa aaaaagcaga
     1141 tcccccttcg actctcaaca aagttatacg ctgcactcgc atcatgggca gaagacgact
     1201 ttcgttcagt caatggccaa atcgaatatc tcctcacaga gtgtgtcaag caacggaaga
     1261 aagacggaaa atacgtatca gaaaccattg acgaaccatt tgagattgat atttaacacc
     1321 ttctcctgtc tgccgtgtcg gataggagaa ttttttcatc tttttttgtc aagtaacttt
     1381 actttacaaa aaaaatgtgt tatcctagta tggttgatga aaatcagtag attgaatcga
     1441 atataaatac ctaaaggaga aatcaaaatg gcagtacctg cacgtcgcac ttcaaaagcg
     1501 aagaaaaaca aacgtcgtac acactacaaa gtaacagctc catctgtaaa ctttgacgaa
     1561 actactggag attactcacg ttctcaccgt gtatcactta aaggatacta caaaggacgt
     1621 aaaatcgcta aagctgcatc agctgaataa tagaagggag ataccatgcg cgtaaatatt
     1681 acacttgaac acaaagaatc tggtgaacgc ttgtacctta cttctaaaaa caaacgtaac
     1741 actccagacc gccttcaatt gaagaaatac tcaccaaaac ttcgcaaaca cgttgtgttt
     1801 acagaagtga agtaagcatt caatacgaat caatacagaa gaaaaacgct tgatcagcgt
     1861 tttttctttt tgttaatttc actatattgc aagagaaatc attcgaatga ctacattttt
     1921 tgaaataaga atacggattc ggacgattaa aagtaagaga aactcaaatg taagttgggg
     1981 ttcctttttt gtatattttt caaataacgc ctctacacta tttgtagcaa attcaccaac
     2041 tacagttgca tcttagttaa aataagttag aatatgtaag tgagtaccag atataccaag
     2101 acatcgtcac catctaaggt atattcaaaa tacaaaagtt gaccaactag atttctgaat
     2161 atccttatat atccattctt aaaattggtt taaatagcgt agtcttttaa actagttttg
     2221 agaatccaaa aaatcttcct acatatgtaa gaagattttt tagttcagaa tgattagatt
     2281 tagctaatgg atacctatcc tacccactca ccgttagcat tgactcgata gccatcgggt
     2341 gtagtcgtgt ttactaataa atctcctgaa ttataggtat agtaccattt accagaaact
     2401 gtaaaccaac cagtagccat tgaacctgat tc
//
LOCUS       DAVKWS010000114         2378 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKWS010000114 DAVKWS010000000
VERSION     DAVKWS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.115"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..249)
                     /locus_tag="VW852_002080"
     CDS             complement(<1..249)
                     /locus_tag="VW852_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273134.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESF"
     gene            complement(246..599)
                     /locus_tag="VW852_002081"
     CDS             complement(246..599)
                     /locus_tag="VW852_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV0273135.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(655..1557)
                     /locus_tag="VW852_002082"
     CDS             complement(655..1557)
                     /locus_tag="VW852_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273136.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMNL
                     TFFVLILSKIILDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSV
                     DDLKSRMIWSFILEIAIILVFILKIPFAIIMLTTIFFWNIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(1765..2139)
                     /locus_tag="VW852_002083"
     CDS             complement(1765..2139)
                     /locus_tag="VW852_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV0273137.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          843 a          439 c          306 g          790 t
ORIGIN      
        1 aaaactttct gggaaaaact tattgattaa aatggggaaa ctcattaaca gaatcgaatt
       61 tatggctaga cagatagata ctattttatt tttcacagaa tatagaggta gtgatactat
      121 tgagtagata aaagctaaaa aaataagcaa aaaatactcc gtatcattta taatcgccct
      181 gaatgataaa aatatataca cccaacacac gatgatgtgt atttttcgaa tcttatctac
      241 attactcatc ttactgttgc agatatgaat ccatcccaag gataagggtt cccgctaata
      301 tcacaacctc tattaagaac accatatcca atacagtaag ctattttccc tgctgctgta
      361 gccaatacac ctgctgctgc accgataagc cagcctccaa gacctccaac cttaatatag
      421 tttattagtt ttttggttaa aactgcagct ccagctaatg ctataaaagt tcctgaactc
      481 atagctaaag ctgcgcaaat tccttgacaa gcacgcttgc ttaaataagc tcccccatca
      541 agatacatca tttcttcttc atcaatcaca acataattat ttggtaatac aagttccatt
      601 tgttttctcc tccttagttg aaattgtttc atttgtttta caaataggac gaatttaaac
      661 tatcttttta ttactcagta ataatttccg agatttcatt ttatgatact ctctattcat
      721 acagaaatca aaattttgcc ttaaccaact tgtataatag aaaaaatcta attgattaat
      781 cttgataagc tctatcctaa ttttatcaat cttttctgac caattatgat tcttactaat
      841 agaaatttca tacagcagta actcgacaag acctgtacac agaagggtgt tatgagaatc
      901 ttcgattttt ttcaaaatat cctcaaaaag ttcactatcc gttaaaaagg tggattggtt
      961 catcgcatca tagagaagaa gggacattaa gtcagaatag cttaattctg atttttgtat
     1021 tttgtccatc aatatcttat gcaaattact ttcaaatgaa tcttcttttt gccatttgtt
     1081 ttgattaaaa agaaattccg tcttattaaa aacgagatga ttgatatcca ttatattcca
     1141 aaaaaatatt gtagtaagca tgatgatcgc gaaaggaatt tttaaaataa atactaggat
     1201 aatagcaatt tcaagaataa aactccatat cattctagat tttaaatcat caaccgacaa
     1261 actttttcga tggaatgaaa aatagggcga gaacctcaca tttactttct tcctgcctat
     1321 aaaaataaaa ggaaaaatcg taataagagc ttctttcttg tacaaatctt tcaatgcgta
     1381 tagtatgtct aaaattattt ttgataatat caaaacaaaa aaagtaagat tcatactaat
     1441 cacaaaatca aggggagatt ctagattcaa tagtaagtta ttcactagga catgcaactc
     1501 tagccacaag acaaaaagag ggagcattaa taaaaaaatg attagtttac gtatcataat
     1561 tttacagtgt ccttatcatt ttcttaattt tggataaatt atcattgcta tgattaaaat
     1621 caaaaaaacg acattcatcc aaataatcaa cttttttatt tcttcgctaa caaaatcata
     1681 ggctaaaaaa gttatggatg atataaatag acatacaaaa aaaataataa caaaaatttt
     1741 ttccatttta atacccatat ctagttacca caaggcgttt aaatttgggt ctctcctatt
     1801 tacatcattg ttggcccaga ttgttgtaat agcatcgcca attgacatcc aagctgcaaa
     1861 tccattaata gcagacagtg caaatcctgc cactctattt gcaactcgta ccccaacata
     1921 tcttgcagct gctccagcaa ttgcgcttgt aactcgccgc aaaccataat tacgaatata
     1981 atttctaact agactgattg ctcctccacc taaaacacca ttaaaagcta tattaatggc
     2041 tgttgcaaca ggccatctca gaatagaaaa tcccccatca agatacatca tctcttcttg
     2101 ctcaagagca acataattat ttggtaatac gagttccatt tattttctcc ttttttatta
     2161 agcatagtta ggatagtttc ctaacaagtg atagacgact gtgtttttat cacttagcta
     2221 cctagataag atatcctttc tctaccttat ttgcttttat aggttaccta aggtttaaaa
     2281 tcaattattg tttgaaactt aacagttcta aaccactagc aataaatgat tgcgaactca
     2341 ctaatactat ttcaaaattt atttttttga cttagtaa
//
LOCUS       DAVKWS010000115         2355 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKWS010000115 DAVKWS010000000
VERSION     DAVKWS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.116"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            179..1660
                     /locus_tag="VW852_002084"
     CDS             179..1660
                     /locus_tag="VW852_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0273138.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2022..>2355
                     /locus_tag="VW852_002085"
     CDS             2022..>2355
                     /locus_tag="VW852_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0273139.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEI"
BASE COUNT          827 a          318 c          388 g          822 t
ORIGIN      
        1 ctgtattcac gccctcaata gacggtaaaa taagaaaatt gtttatatat tgtctccgta
       61 gtgttattat acgaaataaa agattttagg aaaagtcgtt atatcatgct atacctattc
      121 tttgtggtat aattgcaaga ggtttaatcc gataatttat aaagagagaa agatattcat
      181 gagagattta ttatctaaaa aaagtcatag gcaattagaa ttattagaat tattatttga
      241 acataaacgt tggtttcatc gttctgaact agcagagtta ttaaattgta cagaacgtgc
      301 agtcaaagat gatctatccc atgttaaatc tgcttttcct gacttgattt ttcattcttc
      361 tactaatggt atacgcatta ttaataccga tgatagtgat attgaaatgg tttaccatca
      421 tttctttaaa cattcaactc atttttcgat tttagaattc atcttcttta atgaaggttg
      481 tcaagctgag agtatttgta aagaatttta tatcagttca tcttcgctct atcgtattat
      541 tagccaaatc aataaagtga ttaaaaggca atttcaattt gaagtcagtc tgacccctgt
      601 tcaaatcatt ggaaatgaga gagacattcg ttactttttt gcacaatatt tttcagaaaa
      661 atattatttc ctagaatggc catttgaaaa tttttcatca gagccactat ctcaattgtt
      721 agaattggtt tataaggaaa caagctttcc aatgaatttg tcaacgcata gaatgctaaa
      781 gttgctccta gttacgaacc tatatagaat aaagtttggt cattttatgg aagtagataa
      841 agattctttt aacgaccaaa gtttggattt tttaatgcag gcagaaggaa tagaaggtgt
      901 tgctcagagt tttgaatcag aatacaatat ctctttagat gaagaagttg tttgccaatt
      961 atttgtgtct tactttcaaa aaatgttttt catagatgaa agtctcttta tgaaatgcgt
     1021 aaaaaaggat agttatgttg aaaaatctta ccatctattg agtgatttta ttgatcagat
     1081 ttcagtcaag tatcagattg aaatggagaa taaggataat ctgatttggc atctgcataa
     1141 taccgcacat ctgtatcgtc aagagttgtt tactgagttt attttatttg atcaaaaagg
     1201 gaatacaatc aggaactttc aaaatatttt tccaaaattt gtttcagata taaaaaaaga
     1261 gctttctcac tatttagaga ctcttgaggt ttgttctagt tcaatgatgg taaatcatct
     1321 atcttatacc ttcatcactc ataccaaaca tttggtgatt aatttgctac aaaatcagcc
     1381 taaactaaag gttttagtta tgagtaattt tgatcagtat catgcaaaat tcgttgcaga
     1441 gacactttct tattactgta gcaataattt tgaactggaa gtttggactg aattagaatt
     1501 atcaaaggaa tctttagaag attcacctta tgatatcatt atttctaatt ttattattcc
     1561 tcctattgaa aataagagac tcatctattc aaataatata aacacggtct cactcatata
     1621 cttgttaaat gccatgatgt ttattcgatt agatgagtaa ccaaaaataa caaaaaaacg
     1681 ctatttcgcg tttgaactgt acctcaaaag ttagacagaa aaaatctaat ttttggggtg
     1741 tttttgttaa gaaaagacct tcaatcattt aagcaaaatg tacatctaga tttatagccc
     1801 tctaaaactc ttgctttttt tgtagaagat acgaaaaaag ccataaaacc aaggcttttc
     1861 ctgttttatg tagatgttta cacatgaaaa ttgaagtatt ttcaaaagct aattggagtg
     1921 attttattca tgatgggata aatgatctgg ccaaaaatga ttgagaaata gttgcttaaa
     1981 aagcatttgc atgatactat atattgaaag gaggtgataa tatggtagaa caaagaaaat
     2041 caattaccat gaaagatgtt gctttagaag caggagttag tgttggaact gtttcacgtg
     2101 taattaataa agaaaaaggc attaaagaag taactttaag gaaagttcaa agggctataa
     2161 aaacattgga ctatatccct gataattatg ctcgaggtat gaaaaagaat agaacagaaa
     2221 ctatcgcttt gattattccg agcatatggc atccattttt ttcggagttt gcctatcatg
     2281 ttgagaaaga gttattaaaa tataattata aattatttat ttgtaacgct gatagcgatt
     2341 cacaaaatga aatcg
//
LOCUS       DAVKWS010000116         2296 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKWS010000116 DAVKWS010000000
VERSION     DAVKWS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.117"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..1430
                     /locus_tag="VW852_002086"
     CDS             <1..1430
                     /locus_tag="VW852_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0273140.1"
                     /translation="ICVSLILLWFFISCSYSYLKNQYVKKVIIDLKKGLLLHYLSREF
                     NQIDNRNHSDFLNNITKNSDLIQENLLIPRISLIANLGTLIMSVVAIIYIEWRLALVF
                     LLLSSITIFLSQIPGKLMTKATNNYSLQNSHYLSKMTNFINGFEQIKLLNIQSWTQEK
                     IQGISLEFEESRKTYQFLKDLASTTGILLSFGSQLSCMVAGIFFVRNNLLTIGLLVAS
                     IQLLNGVFAPLQSILYNKNLISSSKSIIDNIQENLYETNHGTFHKTSTIDCDNISTIS
                     ISQLYYEIENRILFDHFSYEFKKGKRYAIIGASGTGKTTLVKLILNYYPKNLYDGEIK
                     INGKQNIDIPSEELYKDIAFVQKNDFLIEGNILENIKLARNLHLTEKLRKSLGFNEDF
                     LRKNLAQSNKIISEGEKQRIDLARFLVKTYSVYIFDEPTSNLDPIKANEIMDYILSIK
                     DAIVIVITHDQNPEILEEFDHVITL"
     gene            complement(1440..>2296)
                     /locus_tag="VW852_002087"
     CDS             complement(1440..>2296)
                     /locus_tag="VW852_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0273141.1"
                     /translation="GETYRKIREEKGISISSLAGAEISKSQISRFELGETEISVFKLL
                     YLLERIGVTLEEFLLICNHYQPSDFNTLIASVKQAAYNEDTQTLLDMVEKEMELFRGT
                     NSHYHKLNAIFIESIVSGMDKNHQLSRQDASYLTNYLFSVENWGYYETLILGNCCRVL
                     SPDLLFRYTKEALKKGKLYSPIPRNRQSLIQLLLNSLIIMIENDLYEESLFLKQATKN
                     ILADSTDFFEQTILLYLDGYLELKFYHNQKSLLKIKEALKIFELFNKTIYKNYKQYFE
                     KHIIHLVD"
BASE COUNT          825 a          348 c          326 g          797 t
ORIGIN      
        1 ctatttgtgt aagtttaatt ttactgtggt tctttatctc ttgttcttat tcctatctta
       61 aaaatcaata tgtaaaaaaa gttattatag atctaaaaaa gggtctctta ttacattatt
      121 tatcgcgtga atttaaccaa attgacaacc gtaaccattc agatttctta aataatatca
      181 ccaaaaatag tgaccttatt caagaaaatt tattgattcc tagaatctct ctaattgcaa
      241 atttagggac ccttatcatg agtgtggttg ccattatcta tatagaatgg cgattagcat
      301 tagtctttct tttattgtcg agtataacga tttttctatc tcaaattcct ggaaagttaa
      361 tgactaaagc aactaataat tattccctac aaaatagtca ctatttgtca aaaatgacaa
      421 actttattaa tggttttgaa caaattaagt tattaaatat tcaaagctgg acccaagaaa
      481 aaattcaggg aatcagttta gaatttgaag agtccagaaa gacatatcaa ttcttaaaag
      541 acttagcttc aactactgga attttactaa gttttggctc tcaactatcc tgtatggtcg
      601 ctggtatctt ttttgtaaga aataacttac tgactattgg actactagta gcaagtattc
      661 aattattaaa cggtgttttt gctccactac aatctattct ttacaataaa aatttaatca
      721 gcagtagcaa gtccattatt gataatatcc aagaaaatct atatgagaca aatcatggaa
      781 ctttccataa aacaagtaca attgattgtg ataacatttc gactatttct attagccaat
      841 tatattatga aattgaaaat agaatattat ttgatcattt ttcctatgaa tttaaaaaag
      901 gcaagcgcta tgcgattatc ggtgcttcag gaactgggaa aacaacttta gtaaaattaa
      961 tactaaatta ttatccaaaa aatctttatg atggagaaat aaaaatcaac ggaaaacaga
     1021 atattgatat tccctcagag gagttatata aagatattgc gtttgtgcaa aaaaatgact
     1081 ttctaattga aggcaatatt ctagagaata tcaaattggc tagaaacctt catttaactg
     1141 aaaaattaag aaaatcctta ggttttaatg aagacttcct tcgtaaaaac ctagctcaat
     1201 caaacaaaat catttcagaa ggagaaaagc aacgaattga tttagctagg tttttagtca
     1261 aaacatattc agtttatata tttgacgaac ctacaagtaa ccttgatcca ataaaagcaa
     1321 atgaaataat ggactacatt ctatccatta aagatgcaat tgttattgtc attactcatg
     1381 atcaaaatcc agaaatattg gaagagtttg atcacgttat taccttatag ataaatggcc
     1441 tagtctacta aatgaatgat gtgtttctca aagtattgtt tgtaattttt gtagattgtt
     1501 ttattgaaga gttcgaaaat ttttaaagct tctttgattt ttaaaagtga tttttgatta
     1561 tgatagaatt tcagttccaa atatccatct aaatatagca aaatagtttg ttcaaaaaaa
     1621 tctgtggaat ccgctaatat attcttcgta gcctgtttta aaaataaaga ttcctcatat
     1681 aagtcgttct caatcattat aatcaaagaa ttgagaagta gttgaatgag agattgtcta
     1741 tttctaggta taggactata caacttccct tttttgagcg cctctttggt atatctaaat
     1801 aataaatctg gagatagaac acgacagcaa tttccaagga tgagcgtttc gtaataaccc
     1861 cagttttcaa cagaaaataa ataatttgta agataagagg cgtcctgacg actcaattga
     1921 tgattcttat ccattcccga aacaatactt tcaatgaaaa tagcatttaa tttatggtaa
     1981 tgagaatttg tcccacgaaa aagttccatt tctttctcta ccatatctag taatgtttga
     2041 gtatcttcat tatatgcggc ctgtttaaca gaagctatta aggtattaaa atccgaaggt
     2101 tgataatgat tacagataag cagaaattct tctagtgtta ctcctattct ttcaagaaga
     2161 tacaataact taaagacaga aatctctgtt tctcctaatt caaacctaga tatttgagat
     2221 tttgaaattt cagcacctgc taatgaagaa atagaaatcc ccttttcttc tcttattttc
     2281 ctataagttt caccta
//
LOCUS       DAVKWS010000117         2209 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKWS010000117 DAVKWS010000000
VERSION     DAVKWS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.118"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(92..415)
                     /gene="tnpB"
                     /locus_tag="VW852_002088"
     CDS             complement(92..415)
                     /gene="tnpB"
                     /locus_tag="VW852_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0273142.1"
                     /translation="MMILLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKYRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(676..975)
                     /locus_tag="VW852_002089"
     CDS             complement(676..975)
                     /locus_tag="VW852_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV0273143.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1140..1547)
                     /locus_tag="VW852_002090"
     CDS             complement(1140..1547)
                     /locus_tag="VW852_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0273144.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            1673..2209
                     /locus_tag="VW852_002091"
                     /pseudo
     CDS             1673..2209
                     /locus_tag="VW852_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          685 a          387 c          429 g          708 t
ORIGIN      
        1 gaaataagaa cagtacaaat cgatcaggac agtcaaattg atttctaaca atgttttaga
       61 agtagaggtg tactattcta gtttaaatcc actatatttg gggagtgata gaaaagccct
      121 tcataagcca gtctacttgt tcagatgtga gagctttgac atccttttct gtactgggcc
      181 aagtcagtct gccgttctca aagcgtttat atagtagcca aaatccttga ccatcccagt
      241 agagggcttt aaagcggtat ttacgtccac cacaaaagag aaagacttga ccggagaaag
      301 gatccaattc aaagtgggtt ttaactacat aggctaatga gtctattccc tgcctcatat
      361 ctgtcttgcc acaaacaagg tgaacttgac ctaaatcact tagtagaatc atcatagtac
      421 aatacctttc ctctgataat tattttttat ctagtatact ggaagttggg gaattaggat
      481 agataccttg ttatgacgcg cttacgtaac ttgtaactag ctgcctagtt tgatctttgc
      541 ttcttcattg attagcagca gatttcaaaa tgataaaaac gtataatagt gggtgtagaa
      601 actcttgtta ctatgcgttt tgttatggaa agacttattg gactttctct caaatcgagt
      661 ttttactcaa ttttcttact tgattgggat tgaaattcca attaatttct ctgagtagag
      721 tgtcttgata ttggcttcat caacagaggt cttatcaatt ttacgtttca agaaaaattc
      781 ttgaatggtt tcgatttcag gctcacgaat agcacggtgt ttgtttgaga tgaggatttc
      841 atagtgaagc ggagcttggg taaaaataac atctgtattc cctgcagaat aaacctcaac
      901 aagggttgca tcggtacttt ctagctgact ttttaccagt tgcgagtgtg agtttgtcgt
      961 attgataagc ttcataatat ttcctccgat tttctaattc tattatagca ctttttgaat
     1021 aaagtcgctt gatttatact caatgaaaat caaagagcaa actaggaaga tagccgcagg
     1081 ctatacttga gtacggtaag gcgacgctga cgtggtttga attttatttt cgaagagtat
     1141 tagccaatct tatgctgttt tttccaagat tcaatggccc atttatggct accacgttta
     1201 aggttttgga tagcctcgtc aatagggaac caggcaatat gattaaagtt ttctagtggc
     1261 ttttgtactt ctttgaaaga agttgcttca tagaggtagg caggattgta gtagtaggta
     1321 tcacgatgac gagaatagaa atattcgtca gcttgtccgt aataggtacc aatttctgct
     1381 gtgaaaccaa gctcttcaat caactcacgc tttagggctt cctgatgatt ttcacctgct
     1441 tcaatttctc cacatggtag gaaccaagca ccatttggtt cttgaacaag aacaatttgt
     1501 ttttgttcag gattagggat aactgcatat acgccatagc gagcaatata gtctgtattc
     1561 gctttttttc tccgaaagtt gggtttgcca ttgcattttc ctcattatct agtatcgtta
     1621 ttattatagt gaaatgaacc aaaaatagta cacaatgtgg tataatcttc ttatggcata
     1681 ttcaatagat tttcgtaaaa aagttctctc ttattgtgag cgaacaggta gtataacaga
     1741 agcatcacac gttttccaaa tctcacgtaa taccatttat ggctggttaa agctaaaaga
     1801 gaaaacagga gagctaaacc accaagtaaa aggaacaaaa ccaagaaaag ttgatagaga
     1861 tagacttaaa aactatctta ctgacaatcc agatgcttat ttgactgaaa tagtttctga
     1921 atttggctgt catccaacta ccatccacta tgcgctcaaa gctatgggct acactcgaaa
     1981 aaaaagaacc acacctacta tgaacaagag ccagaaaaag tagccttatt tcttaagaat
     2041 tttaatagtt taaagcacct agcacctgtt tagattgacg aaacaggatt cgatacttat
     2101 ttttatcgag aatatggtcg ctcattaaaa gatcagttaa gaagaggcaa agtatctgga
     2161 agaagatatc agaggatttc tttggttgca ggtctaacaa atggtgagt
//
LOCUS       DAVKWS010000118         2157 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKWS010000118 DAVKWS010000000
VERSION     DAVKWS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.119"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..1326
                     /gene="phtA"
                     /locus_tag="VW852_002092"
     CDS             <1..1326
                     /gene="phtA"
                     /locus_tag="VW852_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV0273145.1"
                     /translation="EPSPGPQPAPNLKIDSNSSLVSQLVRKVGEGYVFEEKGISRYVF
                     AKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDKAYNLLTEAHKALFEN
                     KGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPERLGKPNSQIEYTEDEV
                     RIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGKDSLSDKEKVAAQAYT
                     KEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPYMVEHTVEVKNGNLII
                     PHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDERPHSNDGWGNASEHV
                     LGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLKEAEVLLAKVTDSSLK
                     ANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSVSKEKIN"
     gene            1484..>2157
                     /locus_tag="VW852_002093"
     CDS             1484..>2157
                     /locus_tag="VW852_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0273146.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QAAQKTENLTPDEVSKKEGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHRESGTPRNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASE"
BASE COUNT          777 a          373 c          433 g          574 t
ORIGIN      
        1 gaacctagtc caggcccgca acctgcacca aatcttaaaa tagactcaaa ttcttctttg
       61 gttagtcagc tggtacgaaa agttggggaa ggatatgtat tcgaagaaaa gggcatctct
      121 cgttatgtct ttgcgaaaga tttaccatct gaaactgtta aaaatcttga aagcaagtta
      181 tcaaaacaag agagtgtttc acacacttta actgctaaaa aagaaaatgt tgctcctcgt
      241 gaccaagaat tttatgataa agcatataat ctgttaactg aggctcataa agccttgttt
      301 gaaaataagg gtcgtaattc tgatttccaa gccttagaca aattattaga acgcttgaat
      361 gatgaatcga ctaataaaga aaaattggta gatgatttat tggcattcct agcaccaatt
      421 acccatccag agcgacttgg caagccaaat tctcaaattg agtatactga agacgaagtt
      481 cgtattgctc aattagctga taagtataca acgtcagatg gttacatttt tgatgaacat
      541 gatataatca gtgatgaagg agatgcatat gtaacgcctc atatgggcca tagtcactgg
      601 attggaaaag acagcctttc tgataaggaa aaagttgcag ctcaagccta tactaaagaa
      661 aaaggtatcc tacctccatc tccagacgca gatgttaaag caaatccaac tggagatagt
      721 gcagcagcta tttacaatcg tgtgaaaggg gaaaaacgaa ttccactcgt tcgacttcca
      781 tatatggttg agcatacagt tgaggttaaa aacggtaatt tgattattcc tcataaggat
      841 cattaccata acattaaatt tgcttggttt gatgatcaca catacaaagc tccaaatggc
      901 tataccttgg aagatttgtt tgcgacgatt aagtactacg tagaacaccc tgacgaacgt
      961 ccacattcta atgatggatg gggcaatgcc agtgagcatg tgttaggcaa gaaagaccac
     1021 agtgaagatc caaataagaa cttcaaagcg gatgaagagc cagtagagga aacacctgct
     1081 gagccagaag tccctcaagt agagactgaa aaagtagaag cccaactcaa agaagcagaa
     1141 gttttgcttg cgaaagtaac ggattctagt ctgaaagcca atgcaacaga aactctagct
     1201 ggtttacgaa ataatttgac tcttcaaatt atggataaca atagtatcat ggcagaagca
     1261 gaaaaattac ttgcgttgtt aaaaggaagt aatccttcat ctgtaagtaa ggaaaaaata
     1321 aactaatgaa aaatgaaagt ctcgataaag aggctttcat ttttattatg tatatatgta
     1381 aaattcttga caagcaatat taaaaagagt aaactattaa ctagttaatt aaccggttta
     1441 ttactttata gtgaatcaaa tatacttaag aaaagaggaa agaatgaaaa tcaataaaaa
     1501 atatctagct gggtcagtag ctacacttgt tttaagtgtc tgtgcttatg aactaggttt
     1561 gcatcaagct caaactgtaa aagaaaataa tcgtgtttcc tatatagatg gtaaacaagc
     1621 agcacaaaaa acggagaatc tgactcctga tgaggtcagt aaaaaagaag gtatcaatgc
     1681 cgaacaaatc gtcatcaaga taacagacca aggctatgtc acttcacatg gcgaccacta
     1741 tcattattac aatggtaagg ttccttatga tgcgattttc agtgaagaat tactcatgaa
     1801 agatccaaac tataagctaa aagatgagga tattgttaat gaggtcaagg gtggatatgt
     1861 tatcaaggta gatggaaaat actatgttta ccttaaggat gctgcccacg cggataacgt
     1921 ccgtacaaaa gaggaaatca atcgacaaaa acaagaacat agtcaacatc gtgaaagtgg
     1981 gactccaaga aacgatggtg cggtagcctt tgcacgttca cagggacgct acaccacaga
     2041 tgatggttat atctttaatg cttctgatat cattgaggac acgggtgatg cttatatcgt
     2101 tcctcatggc gaccattacc attacattcc taagaatgag ttatcagcta gcgagtt
//
LOCUS       DAVKWS010000119         2104 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKWS010000119 DAVKWS010000000
VERSION     DAVKWS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.120"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(1..1132)
                     /locus_tag="VW852_002094"
                     /pseudo
     CDS             complement(1..1132)
                     /locus_tag="VW852_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880290.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1134..>2104)
                     /locus_tag="VW852_002095"
     CDS             complement(1134..>2104)
                     /locus_tag="VW852_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HEV0273147.1"
                     /translation="FVEKNTHSVSNLLFKDKYFMRSFLVGVVNQPYFRLLESENDLRV
                     FWNNCKSSAAILKPRNEAGSNGIKKVYPNEKIDSVYFEKNYIIEEYIDIDNMITCDGY
                     SIGSEIKRFYVHEYEELLFNTLSSSGYYLVRTSSLYNDSEKIEYIEKALEECRKVLNV
                     FSINSEITPFHFEWFLELSTGKIVFCEVGKRFGGGDIPQLIEDSHGVDVIKEYWDILT
                     NSQGLKKINFSKVISRPANISATFALYRKQGKVIEVPDEKELAWAKKVYIFIKPNTYY
                     KVSENVVENSMLVQFNCKNSCEFEKRLDELKHISQKFKYVITDSGE"
BASE COUNT          756 a          367 c          277 g          704 t
ORIGIN      
        1 taaaagtatt gttaggattt aaaactactg agaaaaaaac agtacctagt ggcataaaaa
       61 gaatagctac cgtaaagatg attccaaata ctctgccaag aaactcatta tctacgtctc
      121 tttgaacaat agagaaaaat tggatattaa aaatggaaag aaatagacta aataatgctg
      181 gagtaaaggc taaaataatg acgttgcgga acatataata gatcggcgct gttagcatta
      241 acatcaaacc tgaatatgct aaaaagagca ttaaacgttt gcttgacaag gatttattga
      301 taaatccact taatatcgca ccaataaagc caccaatcgc ttcggctgtt aaaaaggttc
      361 catatagccc acttgaaata cttccaaaca tttgattact gtatggcagc aacaaattat
      421 aggctgctag gaaaaagtta acaagtgctg aaagggcaat tatagtgaga atttgtttat
      481 gactatatac atacgtaaat cctattttta aatcatcaaa gattcctctt atcgtcatat
      541 tctcttttgt atccacctcc tcatttacag gagtaatgaa gaaaattagt gaggcagcaa
      601 ttaaaaacga taatccatct agtaataaca caccatgtat tccaagtaac ttatataaga
      661 aaattgcaat cataggaatt gttactttaa tcacagtact tgttgtttct agcaatgaat
      721 taagttatga tatactgtcc ttttttacaa tttctttcgt aaaagctttg tatgatggcc
      781 ctgaaaaagc actcataaaa gccaaaatga catttgttat gactatcgca taaaccaacc
      841 atttttcttg ggatataaat gaaagagtta tgcaagcaat accacataag atatttgtag
      901 taataataat ttttttccgc ttgaaactat ctgcaataac tccaccaaat aaattaaata
      961 gaacacctat catactttct aatgattgat aaattgcaac cactgataga gatgttggat
     1021 taagaccagc aagaaaggta ttattggcga aatcaaacat gatatctccg attcgagata
     1081 tcatcctact agctacaatg gatagaatat tgtgatgttc ttctctaatc attttattct
     1141 cccgaatcag taatcacata tttaaacttt tgtgatatgt gcttaagttc atctaacctt
     1201 ttctcaaatt cacaactatt tttgcaatta aattggacca acattgaatt ttcgactaca
     1261 ttttcagata ctttatagta agtatttggt ttaataaata tgtacacttt ttttgcccaa
     1321 gccaattctt tttcatcagg tacctcgata accttacctt gctttctata taaagcaaac
     1381 gttgctgaga tattagcagg tctagaaatc actttagaaa aatttatttt tttcaatcct
     1441 tgtgaattag tcaaaatatc ccaatattct ttaattacat caactccatg actatcctca
     1501 attaactggg gaatatctcc ccctccaaat ctttttccaa cttcacaaaa gactatttta
     1561 ccagttgata attcaaggaa ccattcaaaa tgaaaagggg ttatttcaga attgatagaa
     1621 aaaacattca aaactttgcg acattcttcc aatgcttttt ctatgtattc aattttttca
     1681 ctatcattat ataacgaact agtccttaca agataatatc cactagaact taaggtattg
     1741 aacaatagtt cctcatattc gtgaacataa aatcgcttaa tctcacttcc aattgaataa
     1801 ccatcacatg ttatcatgtt atcgatatca atgtattcct ctataatata gtttttttca
     1861 aaatacactg aatcaatttt ctcgttaggg taaacttttt ttattccatt acttcctgct
     1921 tcatttctag gttttaaaat tgcagcagaa gatttacaat tattccaaaa aacacgtaaa
     1981 tcattttctg attctaaaag cctaaaatac ggttgattta ctactccaac taaaaatgaa
     2041 cgcatgaagt atttgtcttt aaataataaa ttagacacag agtgagtatt tttttctaca
     2101 aaat
//
LOCUS       DAVKWS010000120         1924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKWS010000120 DAVKWS010000000
VERSION     DAVKWS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.121"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..153
                     /locus_tag="VW852_002096"
     CDS             <1..153
                     /locus_tag="VW852_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273148.1"
                     /translation="GIDLSIEIDKLIKQGEKPSVIAQIKKFQDQVNKDNEYREMMKNK
                     KMDQER"
     gene            227..400
                     /locus_tag="VW852_002097"
     CDS             227..400
                     /locus_tag="VW852_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273149.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            586..1113
                     /locus_tag="VW852_002098"
     CDS             586..1113
                     /locus_tag="VW852_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV0273150.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1240..>1924
                     /locus_tag="VW852_002099"
     CDS             1240..>1924
                     /locus_tag="VW852_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV0273151.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWY
                     EEDKDKLRQK"
BASE COUNT          974 a          261 c          320 g          369 t
ORIGIN      
        1 ggtatagatt tatccataga aatagataag ctaataaaac agggagaaaa accaagtgta
       61 atagcacaga ttaaaaagtt ccaagaccaa gtcaataaag ataatgaata cagagaaatg
      121 atgaaaaaca agaaaatgga tcaagaaaga taagacctgg taaaaatatc ttatccaagc
      181 tataatatga ccaaacaact aaagtcagat ctaggaggta atagagatga ataaaaggca
      241 agagctaata gatgaactca taaaagcaaa tgaagatgga acatacaaaa cctataaaag
      301 cacagaagaa ataaaagcaa tgaacaatga agaaatacag attatatatt ctaacatgaa
      361 aaactatcta tcagacaaaa gaacacacat aaactattaa aggtggaaag gtagtgtaag
      421 ctatttttaa aagtataaaa ggtacaaaga cctaagaaat atataaaaac atcaaaataa
      481 agtattccac caccaagaac aataaaataa aaacaagcaa agggaagtat agaaaaatta
      541 aaacctatac ttcccttttt taattcaaac aaaggagaga aaagcatgat aaacgaagaa
      601 ataagcaagg aagcaggtca agcagcacaa accataatat catacacaat aaaggcaaca
      661 aaagaatcaa tcaatttaga aaaagaaata agaaaaaaga tgaatgaaac tttagaaaaa
      721 gcaaatggaa acttaaaaag tcttatgggc gatgaaatga aaataaaaga cctctacaag
      781 aaaggacaac tagaaaatat aagcatagat caaatcgacc tcaaagactt aaaaaaagaa
      841 ctaaacaaac ttggagtaag tttctcagta atgaaaaaca aagaaagcaa aaactatgaa
      901 atattcttcc aagccaaaga cataaaagta atggaatatg cctttaagca agtcatagcc
      961 aaggaaaata aaaaagaaaa agaaagtatc ctaaaacaaa taaagaaata caaagaccta
     1021 tccaaaaaca aagataagac aaaagaaaaa ggaaaaagga aagtaaaaga aaaagtaaaa
     1081 ccaaacaaaa aagatatgac cagagaaatc taaagggagt aacgaaaagt tacaccctta
     1141 aataaccata ataacaagac ttccgaaaaa cagaagtcca gaattccgaa aatcggaaat
     1201 caagaatttc gattatcgaa aatcaagaaa ggagcagata tggcaacaaa caaaagatac
     1261 tattggataa aattaaaaga agaatttttc acagacaaaa ggataaaaag gttaaggaga
     1321 atatctggag gagataccta tacgataatc tacctaaaac ttctactact aagcctaaaa
     1381 gatgagggca aactctatta tgacggagta gaaagtgatt ttataaaaga gttagcacta
     1441 accatagatg aaacagacga tgatgtaatg gtaacagtaa attatttaat caatcaaggc
     1501 ttattagaag ttgtaacaga aaatgatgaa tactacctaa ctgaaatacc aaacttaatc
     1561 ggatcagaaa cagcctgggc agagaaaaaa agaagataca gacaaaataa acagaggaca
     1621 ttgtccttaa tgagtccaac ccatgtccga caagagatag agatagagaa agatatagag
     1681 atagataaag agagagaggg agagatagat aaaaagtcca cccccatttt ttatggagaa
     1741 ttcaaaaatg taaggttaag caaagaagaa tataaaaatc taaaagaaaa attaaactca
     1801 cacacagata taatgataaa taaactatcc agatacatgg aaagcagtgg taagacctat
     1861 caaaaccact atgtgacaat cttaaaatgg tatgaagaag ataaagacaa actaagacag
     1921 aaag
//
LOCUS       DAVKWS010000121         1810 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKWS010000121 DAVKWS010000000
VERSION     DAVKWS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.122"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(279..914)
                     /locus_tag="VW852_002100"
     CDS             complement(279..914)
                     /locus_tag="VW852_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273152.1"
                     /translation="MTYTLEWLKTFDGEIDILNIKMNCLANTIEKNVSQYKYIYQTNM
                     ENCPEIILSVPNSSIPHLLGLSREHHVNLPTNNAGSIFEGLKDDWTLESLNKADNGWF
                     NENKFKIVGTLLLYQMLHVMECKFYTTDGILNRRVGSRFRRDNIYFVVFKLSNGISYT
                     VELSREQGNQYFPRSLKINDTSVTDCREIDLRLINKIRFKPVKARKVKWPS"
     gene            complement(1204..1791)
                     /locus_tag="VW852_002101"
     CDS             complement(1204..1791)
                     /locus_tag="VW852_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0273153.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYSSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQGTTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTILAWCLYV
                     SLGMFLGLVRYKSDLWGSMGLHLVWNLLVYSLLLF"
BASE COUNT          629 a          348 c          254 g          579 t
ORIGIN      
        1 catttagttt tttatttcaa actgaatcta taagtaagct agaccaccta ttataaagaa
       61 gaagtaagcc atggactaat agtttccact ccaaatgaag ccctagaaac atttctagta
      121 aaaacataca aacagcaaga taaaatgctt gtcaaagaaa ctctgatatc taattttctg
      181 attaactttc ataaaatttc tcgatacaga aattcttcga caatactcac atttgttaag
      241 aaaagaatag tgatgtaatc atgatttcaa tttttaaatc atgatggcca tttcaccttt
      301 cttgccttaa ctggcttaaa tctaatctta ttaatgagtc taagatctat ttctctacaa
      361 tctgtaacgg aggtatcatt gattttaagg cttcttggaa agtattgatt accttgttca
      421 cgtgatagtt ctactgtata actaatacca ttacttaatt taaagactac aaaataaata
      481 ttatctcttc taaatcggct accaactctt ctattcaaaa tgccatcagt tgtatagaat
      541 ttacactcca ttacatgtaa catttgataa agcaacaagg ttccaactat tttgaattta
      601 ttttcgttga accaaccatt atcagcttta ttcaggcttt ccaatgtcca atcatctttt
      661 aatccttcaa aaatactccc tgcattatta gttggtaaat taacatgatg ttctctagat
      721 aatcctaata gatgaggaat ggaggagttt gggactgata gaataatttc aggacagttc
      781 tccatatttg tctgataaat atatttgtac tgagaaacat ttttctcaat agtattggcc
      841 aagcaattca tcttaatatt gagaatatca atctctccat caaaagtctt tagccattcc
      901 aaagtataag tcatttgaat tttctcctaa aaaagagaag gaagctcctg cttccttctc
      961 tcccacgtat tgagtttcac ctcacgctag atatgcccta tctattcctc aagggtaggc
     1021 agtcctgcta ttctttttct caacaaacag tctgaactga gagcggagcc cacaggcggg
     1081 tgcagaccat atccactagc ccacaagtaa ttatatcatt taattatctt cattatacgc
     1141 aattagtcca tatttgtcaa caattacatt cattatgaat atctaaaaat caggtcacct
     1201 attttaaaaa agcagcaaac tataaactag taggttccac accaaatgta gccccatact
     1261 gccccataag tccgatttgt agcgtacaag ccctaaaaac atcccaagtg aaacatataa
     1321 acaccaagct agaatggttc ctggatgatg tgctaaggca aataaaacac ttgtcaaagc
     1381 aactcgaata tctaattttc taaccaagtt ccataaaatt tctcgataca gaaattcttc
     1441 aaccatactc gcattgatta agaacaataa aaatgaaaac caagggactt gatgttgaag
     1501 gccaattaag tttgcttgat tcgtagttcc ttgagcatgg attaggctaa aacatagact
     1561 tataatcagt aggctaacga atccaatacc aagccatttc atcctagatt tcatattgac
     1621 cttatgagct tgtttgcgtt gaccatacat ccataaaaaa gaaatgagtg acgaactata
     1681 gagaatctgt agtatagtta actcaccgat acaaagaaat ttcaataagt atagagatac
     1741 caataggaca tttacttgtt ggaatatata aactggaatt attcttttca tagttacctc
     1801 cgaaataaat
//
LOCUS       DAVKWS010000122         1784 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKWS010000122 DAVKWS010000000
VERSION     DAVKWS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.123"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(112..360)
                     /locus_tag="VW852_002102"
     CDS             complement(112..360)
                     /locus_tag="VW852_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273154.1"
                     /translation="MLYWISVNYEITEDEDFETVKTRVISDFESYLKLLNGKFEESLK
                     KVIKSFTFSKLIGEKLCNGEDLKNLSKEIRHQIQDRNS"
     gene            520..996
                     /locus_tag="VW852_002103"
     CDS             520..996
                     /locus_tag="VW852_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273155.1"
                     /translation="MSRTYINIRLAYETKYWLECIQEEVQKKLDALINEQDIEELESI
                     ITNKLEKKDSLLGAVSITTFFKVSSSSVIEKAYNETKNYTTSEWREIARQMNLAKVKQ
                     NMEIGTLTPRLYLDNNIISELEKYQFLFKSDDMIRQVRMSYVIKMVAFAYYKKLFA"
     gene            1167..1721
                     /locus_tag="VW852_002104"
     CDS             1167..1721
                     /locus_tag="VW852_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273156.1"
                     /translation="MPKLLETEKYSLDSIIDGGKLRMISKFCPDLHGLRYEFKTSDSI
                     TKEYCKKIRQALRDSDPEGKSGKKCMMRYTIDILNVWNTLCRTRDFITGSLKADDVID
                     GKTGIYFFDVNTSNVITDDVIENVKINHKSLVRNVDEENIESISKEIPKGTDMYYYVL
                     YRLGLNRIKYNYLVKALAGAIQKD"
BASE COUNT          674 a          205 c          304 g          601 t
ORIGIN      
        1 ttttgagagc agtgaaatta gcagacttac gtcataattc agattaattc aatgttttaa
       61 gaaattagtt atactataaa aacaataatt attttagttt ttagtaattt tttaggaatt
      121 cctatcttgg atttgatgcc ttatttcttt acttaaattt tttaggtctt caccattaca
      181 tagtttttct ccaatgagtt tactaaaggt aaaagactta ataactttct tgagactttc
      241 ttcaaacttg ccatttagaa gcttcaaata gctttcaaaa tctgatatca ctctcgtttt
      301 gacggtctca aagtcttcgt cttcagtaat ttcataattg actgatatcc aatatagcat
      361 tttctatctc cttatttagt tttattatta ctagtttatt atatttttaa tagaatgtcg
      421 caatttaatt atatagaatt taaattcaaa aatattgttg acaaatagaa aatactgtgt
      481 taaaatgtct atataacaga gacagttgga ggtataagaa tgagtcgtac ttatataaat
      541 ataagacttg catacgaaac caagtattgg ttagaatgta tacaagaaga ggttcaaaaa
      601 aaattagatg ctttaattaa tgaacaagat atagaggagt tagaatcaat catcactaat
      661 aagttagaaa aaaaagattc tctattgggg gctgtttcca taacaacttt tttcaaggta
      721 tcttcttcaa gcgtgattga aaaagcctat aacgagacaa aaaattacac tacttcagaa
      781 tggcgagaaa ttgcaaggca aatgaatttg gcaaaagtta agcaaaacat ggaaattggg
      841 acgttaacac ctagattata tttagataat aatattattt cagaattaga gaagtatcaa
      901 tttttattca aaagtgatga tatgataagg caggtaagga tgagctatgt tattaaaatg
      961 gtcgcatttg cctactataa aaaattgttt gcatgattta taagctcgat aataagagct
     1021 tataaaaata caacctttgt ctgtgttaac gagactttta aactttttaa tcggaaggag
     1081 aatattttga taggagcgtc acctattatt tatttgacaa cattaaataa aaactaatat
     1141 taaaaaaata taaaggagga gcagaaatgc caaaattatt agagactgaa aaatacagtt
     1201 tagatagcat tatagatgga gggaagctta gaatgatttc taagttttgc ccagacttac
     1261 atggcttgag gtacgaattc aaaacatcag actcaattac taaagaatat tgtaaaaaaa
     1321 tacgtcaagc tttaagagat tctgatccag aaggaaagag tggaaaaaaa tgtatgatga
     1381 gatatacaat agatatttta aatgtatgga acacgctatg tagaacacga gattttataa
     1441 cggggagttt aaaagcggat gatgttatag atgggaagac agggatatac ttctttgatg
     1501 ttaatacgtc taatgtaatt acggatgatg ttatagaaaa tgttaaaatt aatcacaaaa
     1561 gcttagtacg aaatgttgat gaagaaaata tagaaagtat ttcaaaagaa attcctaaag
     1621 gcactgatat gtattattat gttttgtata gattagggtt gaataggatt aagtataatt
     1681 atttagtcaa ggctctggca ggcgcaatac agaaggatta agaatataat tatataatta
     1741 agggggagtt ttttctcccc ttatagagat agtattattc tttt
//
LOCUS       DAVKWS010000123         1755 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKWS010000123 DAVKWS010000000
VERSION     DAVKWS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.124"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..938)
                     /locus_tag="VW852_002105"
     CDS             complement(<1..938)
                     /locus_tag="VW852_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV0273157.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNS"
     gene            complement(951..1700)
                     /locus_tag="VW852_002106"
     CDS             complement(951..1700)
                     /locus_tag="VW852_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV0273158.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQSSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKIYQEEFDKKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIIFWIKK"
BASE COUNT          604 a          321 c          203 g          627 t
ORIGIN      
        1 gaattatatt tcatccccaa tttatatatt cctactatct ttggctttcg ttccaatatt
       61 tgtttaacta tgaactgttt tctatttgtg tttgaaatat caatcgcttc tatcactggg
      121 gcatttattt ctataaattc tttttttaat tgtttagtat ctttgggaag acaatatcct
      181 ccaaatccaa aagaaggatt attataaaaa tttccaattc ttggatctaa acaaacacct
      241 tttattacaa cttcagcatt taaacttctc ctctcagcaa aagaatctag ttcattaaaa
      301 aaagcaacac ggagagctaa gaatgtgtta gaaaaaagct taattgcttc tgcttcagta
      361 ggagaaacta acataacatt tttaatattg gcagtactat gagtactaat cgaaaggaac
      421 aactctgcaa tttttcttcc ttcaactgtc tcatctccaa caactatgcg acttggatat
      481 aaattatcat atatagaaca accttctctc aaaaattcag ggacaaaaat gatatttttt
      541 gtatcaaaca gcctttttaa tttgtttgaa aagccgatcg gaactgttga ctttaaaata
      601 atctttccat taggttttac cctcagaatc ttcgataccg tttgttcgat ttcatatgta
      661 ttaaaactac caattttctc atcataatct gtcggaagcg caataatata ataatcaata
      721 ttatttttaa tttcagaaaa tgtatcaaaa aaagtaatat ttaagttatt ctcgcaaaaa
      781 aacttcataa gctcttcatt tttagatgga agaatgccct tttttaaatt atttattttt
      841 acagaatcta tatcatatgc aacaacttta tatttagatg caaatagtaa cgcgtaggcc
      901 agcccaacat gccccaaacc aattactgct atattcataa aactacttcc ttatttctta
      961 atccaaaata taatagaata agctgcccca ttccttaaat acaactcttt aatattgttt
     1021 aaaagttttt caactgattt ccagattatc aaaatctgag atttatagca caatattgat
     1081 gatattctat caatataatg ttttttatca aattcctctt gatatatttt taattcttta
     1141 gtttttccca tataactaac catactacta tcacttacat atgggaaatc ctcataatat
     1201 attactttat aacgcataaa ttcaagcgcc cttccaatac tattcacaaa aacatgatca
     1261 acatggtcac caagtgaaag cggacaatat acgacacatt tgtcgtctaa atgcattaac
     1321 agctctttta tgatatcatt ctttaatgtg tcctcatttt ttaattcact atagatatga
     1381 cggtatagaa aattgccatt tctatctttc ctatagagac attcatagta cgataagtgt
     1441 ctaaaatcac attgtagacg ttcacaagct aacctgtctt ctttcttcct ttcttcaatc
     1501 ggatatttcc caaggttaca caacttatga aattgcttag cagaggactg tagctgttgg
     1561 ctcaaagggt aaccagaaaa tatagtaata acaagtacaa tttctccttc tgaagttaat
     1621 tttgaaatat aatcaccaca ggaaaaaatt gcgtcatcta aatgtggaga taaaaagata
     1681 tacttagtat tgttactcat aaccattccc tctacaattt atctaaaaac tcactaagtg
     1741 tctgattaaa ttcca
//
LOCUS       DAVKWS010000124         1504 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKWS010000124 DAVKWS010000000
VERSION     DAVKWS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.125"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            18..>1504
                     /gene="pcpA"
                     /locus_tag="VW852_002107"
     CDS             18..>1504
                     /gene="pcpA"
                     /locus_tag="VW852_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV0273159.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYL"
BASE COUNT          582 a          204 c          251 g          467 t
ORIGIN      
        1 tatgtaagga aatttaaatg aaaaaaacta caatattatc attaactaca gctgcggtta
       61 ttttagcagc atatgtccct aatgaaccaa tcctagcaga tactcctagt tcggaagtaa
      121 tcaaagagac taaagttgga agtattattc aacaaaataa tatcaaatat aaggttctaa
      181 ctgtagaagg taacatagga actgttcaag tgggtaatgg agttactcct gtagagtttg
      241 aagctggtca agatggaaaa ccattcacga ttcctacaaa aatcacagta ggtgataaag
      301 tatttaccgt tactgaagta gctagtcaag cttttagtta ttatccagat gaaacaggta
      361 gaattgtcta ctatcctagc tctattacta tcccatcaag cataaaaaaa atacaaaaaa
      421 aaggcttcca tggaagtaaa gctaaaacta ttatttttga caaaggcagt cagctggaga
      481 aaattgaaga tagagctttt gatttttctg aattagaaga gattgaattg cctgcatctc
      541 tagaatatat tggaacaagt gcattttctt ttagtcaaaa attgaaaaag ctaacctttt
      601 cctcaagttc aaaattagaa ttaatatcac atgaggcttt tgctaattta tcaaatttag
      661 agaaactaac attaccaaaa tcggttaaaa cattaggaag taatctattt agactcacta
      721 ctagcttaaa acatgttgat gttgaagaag gaaatgaatc gtttgcctca gttgatggtg
      781 ttttgttttc aaaagataaa actcaattaa tttattatcc aagtcaaaaa aatgacgaaa
      841 gttataaaac gcctaaggag acaaaagaac ttgcatcata ttcgtttaat aaaaattctt
      901 acttgaaaaa actcgaattg aatgaaggtt tagaaaaaat cggtactttt gcatttgcgg
      961 atgcgattaa acttgaagaa attagcttac caaatagttt agaaactatt gaacgtttag
     1021 ccttttacgg taatttagaa ttaaaagaac ttatattacc agataatgtt aaaaattttg
     1081 gtaaacacgt tatgaacggt ttaccaaaat taaaaagttt aacaattggt aataatatca
     1141 actcattgcc gtccttcttc ctaagtggcg tcttagattc attaaaggaa attcatatta
     1201 agaataaaag tacagagttt tctgtgaaaa aagatacatt tgcaattcct gaaactgtta
     1261 agttctatgt aacatcagaa catataaaag atgttcttaa atcaaattta tctactagta
     1321 atgatatcat tgttgaaaaa gtagataata taaaacaaga aactgatgta gctaaaccta
     1381 aaaagaattc taatcaggga gtagttggtt gggttaaaga caaaggttta tggtattact
     1441 taaacgaatc aggttcaatg gctactggtt gggttaaaga caaaggttta tggtattact
     1501 taaa
//
LOCUS       DAVKWS010000125         1481 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKWS010000125 DAVKWS010000000
VERSION     DAVKWS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.126"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            1..1019
                     /locus_tag="VW852_002108"
                     /pseudo
     CDS             1..1019
                     /locus_tag="VW852_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1230..>1481)
                     /locus_tag="VW852_002109"
     CDS             complement(1230..>1481)
                     /locus_tag="VW852_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0273160.1"
                     /translation="VPAQPGTEDKKPSAPKPDMQPSPQPEGKKPSVPEINQEKEKAKL
                     AVATEKKLPSTGVASNLVLEIIGLLGLIGTSFIAMKRRK"
BASE COUNT          452 a          304 c          251 g          474 t
ORIGIN      
        1 aaaatgatgg ttgctgaaac ttctatcgtc aagaagaatc atcaaatccc tcgtatcatc
       61 aaccaaaaga ttgctcaaaa gttaattgaa aagatttcta tgaccgatat tgcccatcag
      121 ctttccatct caacttcaac tgtcattcga aagctcaatg acttccactt tgagtgtaat
      181 tttagaaatc tgcctaagat tatgtcttgg gacgttgaaa cagtccgggg agtgactgtt
      241 tcaatcggga gatggagatg agcttcattg cgcaagattt taacaatctc aatatcatca
      301 ctgttcttga gggaagaaca caagctatca ttcgaaatca ctttcttaaa tatgatagag
      361 ccgtccgatg tcgcgtcaaa attattacta tggatatgtt tagtccttac tatgacttag
      421 ctagacaact tttcccatgt gctaaaatcg tgttggatcg ctttcacatt gtacaacatc
      481 ttagccgtgc tatgagtcgt gtgcgtgttc aaatcatgaa tcaattggat cgaaagtctc
      541 atgaatacaa ggctatcaag cgctactgga agctcataca acaggatagt cgtaaactca
      601 gcgataaaca tttttatcgc cctacttttc gtatgcattt aaccaataaa gagattttaa
      661 acaagctttt gagctattca caagacttga aacatcacta tcagctctat caactcttgc
      721 tgtttcactt tcagaataag gaaccggaga aatttttcgg actcattgag gacaatctaa
      781 agcaggttca tcctcttttt cagactgtct ttaaaacctt tctaaaggac aaagagaaaa
      841 tcgtcaacgc ccttcaacta cactattcta acgccaaatt ggaagcgacc aataatctca
      901 tcaaacttat caagcgcaat gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga
      961 tttttatcgc tctgaatatc aaaaaagaaa ggacaaaatt tgtcctttct cgagcttagc
     1021 tgacttcaac ccactacagt tgacaaagag ccttaattgt ctaacttttg cggttcacta
     1081 gccttctctt tttatatctt agattcatct ctatatgaaa tgacttataa ttattagata
     1141 ccaattataa gtaaaatctt tgggatcttc aatggttaac caatcaaatg ctaaatatcg
     1201 aaagtggata tttttaaaaa actgaatcat tattttcttc ttttcatcgc gatgaatgaa
     1261 gttccaatca aaccaaggag accaatgatc tcaagaacta gattagacgc cactcctgta
     1321 gatggcaatt tcttctctgt tgctacagca agcttagctt tttctttctc ctgattaatc
     1381 tctggtacac ttggtttctt accctctggt tgagggcttg gttgcatatc tggttttgga
     1441 gcagatggct ttttatcctc agtaccgggt tgtgccggta c
//
LOCUS       DAVKWS010000126         1466 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKWS010000126 DAVKWS010000000
VERSION     DAVKWS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.127"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..185)
                     /locus_tag="VW852_002110"
     CDS             complement(<1..185)
                     /locus_tag="VW852_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0273161.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVE"
     gene            complement(198..578)
                     /locus_tag="VW852_002111"
     CDS             complement(198..578)
                     /locus_tag="VW852_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0273162.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIGLPFVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(571..894)
                     /locus_tag="VW852_002112"
     CDS             complement(571..894)
                     /locus_tag="VW852_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273163.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEANLL
                     EVSHD"
     gene            complement(908..1156)
                     /locus_tag="VW852_002113"
                     /pseudo
     CDS             complement(908..1156)
                     /locus_tag="VW852_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(1153..1326)
                     /locus_tag="VW852_002114"
     CDS             complement(1153..1326)
                     /locus_tag="VW852_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273164.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQS"
BASE COUNT          470 a          289 c          224 g          483 t
ORIGIN      
        1 ctttctaccc atggactttg aggcattcgt tgttccatct tgtagtggcg aatcttttga
       61 tataaacgat tcaattcact tggatagtga aactctcccg caaacatttt tctggttaac
      121 tcaatccagc tgatatttct ttcagccaaa ataatggaca agttctccca aaatcgttca
      181 gccatattac ttctccttta gttagataaa taatgtgttt gtgccatgta aatcaattgt
      241 ttcgtatctc ttggcaatag agctctagcc tcttccaaat tcagacttgg ataaacccgc
      301 ttatttgaaa ccacaaaagg aagtccgatg gttagttcag gattttttaa aattatctca
      361 acgaaatccg ttaatcttag attgtcacgg ttcttaaatc gtaataaatt gggagataaa
      421 aactcaaaac aatctgaaga atagctcatc atctcaatta atttgtcctt tgtcatttca
      481 gaaactgaat gacaagatac ctcaatgcca tagttttgga agaagtctaa aagaagttga
      541 tttctttggc tatttttact tagatagaga tcaatcatgg gagacctcca acaaatttgc
      601 ttccatttga tattctgaga cgattaagga atctaataaa tttgcgaagt taatcggttt
      661 cttgtcttca tcataagctt ttacagttac ttgggttgta agtattccct cttttccctc
      721 ggctcgatag ccttgtccat ataaaacaaa aacgagattt tgatgatcat ctacaaaggc
      781 atcaacccca ttctttatgt cttgactttc aaggaattcc ataacgtttt gaagatagga
      841 ttcgtaaaat agtgggtaat tatgtttttt atggtaatca tctaaaaatg tcacctcaaa
      901 ctcacatgga gagtaatttt gactttgaac agcctaaaag tgccatcaaa tttgaattgg
      961 aataaatcaa ataaatagcc ccatcctcat caatccaagc tttgctcaaa gacaactcca
     1021 accgatcttt taaaactgag taaaccacct taacctccag tttcatattc ttataccgtt
     1081 cactctcaaa taaaagtttg gggagcttat aataacgctc tgatgtctga tattgactag
     1141 cggtaatacg cttcatgatt gtccctccaa gactaaaatt ccaacatttc caaattcatc
     1201 aaatcggatt aaacctactt gttccatttc atcaactaac tgagttgctt ttacaatatc
     1261 aactcccatg atagtcatga gatgactctt cacgaattga cgtgatgact gtccttcctt
     1321 ttgcataatt acctccgaaa cacaaaaaaa ggggtagaca atctagtgtc tacctccgaa
     1381 aatttattaa aaagaaaaac cctgccaaag aatttttggt agggtttttg gtaggaaact
     1441 aaataaattt atcagtttct aaaatg
//
LOCUS       DAVKWS010000127         1455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKWS010000127 DAVKWS010000000
VERSION     DAVKWS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.128"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(297..1406)
                     /locus_tag="VW852_002115"
     CDS             complement(297..1406)
                     /locus_tag="VW852_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0273165.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          547 a          263 c          215 g          430 t
ORIGIN      
        1 aataagaata ggacgaatag atcaggacag tcaaatcgat ttctaacaat gttttagaat
       61 tagaggtgta ctattctagt ttcaatctac tataaatagg tttcattcga actttaatag
      121 actatgaaaa ccagtggaac ttaccctgac acagaattcc actggttttt tcgattttta
      181 tcagattaac ttctactatt cttagctgtg aaaattagtt taagacttta acttattttc
      241 agtcttctaa tctaaaggcc acttccagta aagaaaaatc agggttctcc tgacttttac
      301 ttagctaatt ctctttctcg ttctttcatt attttatgat tttcatacaa acgggataag
      361 aacttagaaa tttctttcaa gatagaatag gttggcactg cgacaatcat tccaactaca
      421 ccataaatat tgcttgacaa caaaagtaaa actaaaatcg tgattggatg aaccttcata
      481 acacttccta cgattcgagg atataagata ttgccatcta cctgctgaac aacaagcata
      541 taaatcactg caatcagcag tctatgggga tcagtgaata tatttgcgat gatcatagga
      601 atcaaaccaa tacttggccc cacataagga attaaattgg ctacaccaga aaaaatggca
      661 aaaactaaag catattttaa accaataata ctatagccaa tataagctaa acaacctata
      721 atgattgcgt caatcgaaac tccactaata tagcgagcaa tcgtcgcatt taaattcttt
      781 aataagcctg caatatgcaa gcgatccctc tttagaatcg ttctttcaag catgggcaag
      841 aatttatgtc catctaataa gaaataaacc aaaaaaactg gagtcataat caaaatcaaa
      901 acagtactga taagagctga caagacgctc cccacactat ttgatacgct atttaggata
      961 ttttgaagaa tatcaacata ggataagttt aactgctgaa ttgtagcttc tacatccaaa
     1021 ttctggagcg caggataatt agataagtct atgattaagt cttgtactcg actataaata
     1081 gtttgactag atataatcaa actagataac tgattaatca aaataggtaa gagatagaca
     1141 acacctatga ccattcccca gaccaaagta cacaaggtaa ttaaaatacc aagcaaacga
     1201 ttgagtttac agactttatt taagaaagta acaatagggt ttgtcaaata ataaagaaag
     1261 ccccctaata aaaatggaat cataattgta ttaagcacgc taacaaaagg gttaatcaaa
     1321 gaccccatct gtctccatag gtaaaagatg atggttaaga gtaaaatttc tgtggtccaa
     1381 aaaaataatt tatttctacg aaacatgagt tacctccctc actttatttt accatatttt
     1441 caaaaagctt aacag
//
LOCUS       DAVKWS010000128         1448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKWS010000128 DAVKWS010000000
VERSION     DAVKWS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.129"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..220)
                     /locus_tag="VW852_002116"
     CDS             complement(<1..220)
                     /locus_tag="VW852_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV0273166.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAA"
     gene            complement(313..471)
                     /locus_tag="VW852_002117"
     CDS             complement(313..471)
                     /locus_tag="VW852_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV0273167.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(542..970)
                     /locus_tag="VW852_002118"
                     /pseudo
     CDS             complement(542..970)
                     /locus_tag="VW852_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1065..>1448)
                     /locus_tag="VW852_002119"
     CDS             complement(1065..>1448)
                     /locus_tag="VW852_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0273168.1"
                     /translation="IAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEISNWHIKASQY
                     YLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKAEKQGITLYHHDQ
                     CRSGSVVQEFLGDYSGYVHCDILRQ"
BASE COUNT          404 a          322 c          271 g          451 t
ORIGIN      
        1 cagccgcaat ttgttcataa gttcgatatt ctcgcacata ttgaagagtg gccataagaa
       61 ggtcttctag acttaattta ggttttcgtc caccttttgc gtgtttaagt tgataagctg
      121 tttttaatac agctaacatc tcttcaaaag tcgtgcgttg aacaccaaca agacgcttaa
      181 atcgtgcatc ggttagttgt ttacttgctt catcattcat agaactatta taccatattt
      241 tgtttcgcag gaagtctatt gaaaaatctc cagactggag aactcacgga tagttcctaa
      301 tctggagatt tcttatttgc acttttcttg tacaacttta gtccatggta aataagcctc
      361 taaaacctct ttgtttacga gagtttcctc gtttggaaga cattctagaa gataggatag
      421 atatttctcg ctatttaatt gatgacgttt agctgtttcc aacaaactca taataatagc
      481 tcttgcttta gctccttcaa aactttgaga aaataaccac cctcgctttt ttaaactgag
      541 cttaggctaa aagagtccac tggactcttt tactccgtcc cataaccaat gatttaatgg
      601 cgcgttcagc tagattattg gaaaggacca gatgtccgtc tttcaaaatg gtcttaaagg
      661 tttcttcata cttgaggctg tattcaattg cccttcctag ttttgaacct gctaaaactg
      721 actgacgccg gcactaagca aagaagtctt ccattaaggg ctggagatgt tcttgacgtt
      781 tctgtagtcg ttcatcagct ggcaaagcct cccagtctct ttccaaggaa aataactgat
      841 cacaataagc taaaccttta gctcctaagg atgatttatc cgcttgcttg gggggcgctt
      901 caaaaaactt ccttctcaca tgcgcccaac atccaacaag ttcagcttct tccagttgac
      961 gataagcaga cgagcggttc gaagtttacc aagcttagcg tcgccttgct cctaaacctt
     1021 ggactgctat cgcataggca gaactagagg actaaagtcc taagttactg ccgcaaaata
     1081 tcacaatgca catagccaga ataatctcct aggaattctt gtactactga accacttcga
     1141 cactgatcat ggtggtaaag cgtaatccct tgtttctctg ctttacctga caaaaaagtc
     1201 caatagtaag tcagctgact atcactctct agcaccctat aagaagtttc atccgcatga
     1261 agtaagggct gagtcaatag tctctctcgc aagaggttat aaaggggctc caaatagtat
     1321 tgactcgcct tgatatgcca attagagatt tccttacgtg tgattggtaa acccatccta
     1381 gcccaatctt cttcttggcg ataattgggt accttcagat taaacttctg atggatggtg
     1441 tgagcgat
//
LOCUS       DAVKWS010000129         1434 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKWS010000129 DAVKWS010000000
VERSION     DAVKWS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.130"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            143..>1434
                     /locus_tag="VW852_002120"
     CDS             143..>1434
                     /locus_tag="VW852_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV0273169.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQILN
                     SVTGDEKKIIEFFRDSILNFNFLYRKLETINELFDEIR"
BASE COUNT          544 a          167 c          253 g          470 t
ORIGIN      
        1 ggaagaaaat tataatttat ctataactca aataaagaat tctattaaag aaaattcctt
       61 agttttgttt gttggtgcag gaatatcagc taattctaat ctaccaactt ggggagaatt
      121 gattcaatca ttaaaaaaag aattgaacat accagaagaa agaacagata gtccactgag
      181 aattgctcag tattattatg atacttttgg gaaaaatcag tacacaaaga agatagaaga
      241 aattttcttt aagaagggat tgagtaaacc aaatgagtta cataaattaa tagaaaaaat
      301 tgcaccaaaa catattatta cgacaaacta tgattcactt ctggagagtc aatttgaaag
      361 tggcttgtta aagtacaatg tagtcgctga ggataaagac atcccttata cgagttcgga
      421 aagatacttg attaaaatgc atggtgactt cagtaagaaa aatattgttt tgaaagaaga
      481 tgattattta gactatcatt tgaacttccc aatgatttct actttaatcc agtcattgat
      541 aatgaatcat actctattgt ttgtggggta ttctctaagt gattcgactt ttaattcaat
      601 ttttagaatg attcaaaata catttaaatt ggatgctaaa aatgcttatt tttatactcc
      661 cgaggaaccg tctatgatca ttcgagagta ttataagaag caaggaattt tcattatctc
      721 taatgaagag aatctaggtc aggagacatc agaaaaacag aacaaattgt attgcaggac
      781 aaaagatttt ttagaggttc tatcagaaaa ccgaagtcaa gatgtaaata atgctgatga
      841 tttatggaat caactggctt ttttagatag acttagtttt attgatgcga aagatttttc
      901 tagatattct gatttaaaga aaagggcttt aaactgggat gatgagtatt gctggtttgg
      961 aaatgatcaa ttaagatttg aaattgatgg tcatgaagaa ttacgaatta tggtatctaa
     1021 aaaatcttta ttaaatcgct ttctagatat ggagattggt gagcctaggg atttaaaggg
     1081 gaatcgattc ttaagtaagg cttttaagtt gtatgaagaa aaacaatatt ctttagcaaa
     1141 agcaaaattt agagaactag ctaatatagc atttcgtcag aaagactatt ttaatttctt
     1201 agtttgtgaa tttaattttc agcaaattca aataattgat agatacgaaa aaacacctag
     1261 ttatgcagag ccactttatg atggagaatt aagcgaatta acagaacaaa ttcttaactc
     1321 ggttacagga gatgagaaaa agattataga attttttaga gattcaatcc ttaacttcaa
     1381 ttttttatat agaaaattag aaactattaa tgaattattt gatgaaattc gtga
//
LOCUS       DAVKWS010000130         1416 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKWS010000130 DAVKWS010000000
VERSION     DAVKWS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.131"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..254
                     /locus_tag="VW852_002121"
     CDS             <1..254
                     /locus_tag="VW852_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEV0273170.1"
                     /translation="LQNQKRQLKKKLNEQERKARNKRLIEKGAVFESIFEESIDLTKD
                     EFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKDEIT"
     gene            564..>1416
                     /locus_tag="VW852_002122"
     CDS             564..>1416
                     /locus_tag="VW852_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV0273171.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLD"
BASE COUNT          666 a          176 c          250 g          324 t
ORIGIN      
        1 aattacaaaa tcaaaaaaga cagttgaaga aaaaacttaa tgaacaagaa agaaaagcaa
       61 gaaacaaaag acttatagaa aaaggtgcag tgtttgaaag tatttttgaa gaaagtattg
      121 atttaacaaa agatgaattt tataagttaa taaaaacgtt aaatgatgaa gaaataagat
      181 taaacataat ggaaatttta gaagaaagaa tagatgataa tgtagaaaaa tcatctaaag
      241 atgaaataac ataattgtcc cttggttaca agggcgcaat tatacaccct aaagggtgct
      301 tgcgtcctgc ggagtcaaac ccttgcaggg agcaacaacc attcgctttt taaaagtcaa
      361 gggtaaataa actcgctaag gctcgccctt gacttttaaa atttgaaatg aaagtaacaa
      421 ttaagccgac atactgaaac aacaaaaatt aattcagtag tcggcttttt ttattctatc
      481 atttcaaaac gctcattcac aaagaggata taaaaaaact attaagcctc cacctaaaac
      541 aacaaagaaa ggaagtgata aaaatggcag acagttttca tttttcagta aacataatat
      601 caagaggaaa aggcaaaagt gcagtagcaa gtgcagcata tataagtgga gaaaaaataa
      661 aaaatgaatg ggacggagta acccatgact acacaagaaa agaaaaaata ttagtaaaaa
      721 atataatatt gcctgatcat ataccaaaag aatttaatga taggtctacc ttatggaata
      781 aagtagaaat ggcagaaaaa aattctaatg cacaactagc aagacaattc ataataggac
      841 taccaaaaga gttatcttta agtgaaaata aaaacctggt tgaaagatat ataaaagaaa
      901 atttaacatc acaaggaatg atagtagatt atgcaattca tgatgagagt caagataaaa
      961 atggaaatat ccattgtcat ataatgacca taatgcgacc aataaacgaa aaaggagagt
     1021 tcttagccaa gtcaaaaaaa gaatatatcc tagatgaaaa aggagaaaaa gtattaaaca
     1081 aaaatggaaa accaaagaca agaaaagtag aactaacaac ctggaacgat acaggcaatg
     1141 tagaaaaatg gagagaaaat ttttcagatc tttgtaacaa atatttagaa agagcaggag
     1201 cagaaaaaag agtagaccat aggagtttta aaagacaaaa ttcagattat ctaccgacaa
     1261 tccatttagg atcagcagca agtgcaatgg aaagaaaggg aatagaaact gacaagggaa
     1321 actataatag agaaataaga aaatataatc aacttgtaaa aacaataaaa gaagagataa
     1381 aaacattaaa gggttggata ggaaatttat tagata
//
LOCUS       DAVKWS010000131         1384 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKWS010000131 DAVKWS010000000
VERSION     DAVKWS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.132"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            88..1266
                     /locus_tag="VW852_002123"
     CDS             88..1266
                     /locus_tag="VW852_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273172.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLMFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLGRLVI
                     "
BASE COUNT          474 a          270 c          293 g          347 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagctgact gcggctagct ttctagtttg ctctttgatt
       61 tttattgagt attagaaagg agacctcatg acacttccag ttagaaaatc cctgcacgat
      121 gcagttttac aggcttcgaa agcggatact tgggaacaag ctaccaagga atggaatgaa
      181 gtttccttga tgtttaacgg tatcggccgt agcaattgtg tctgtggaaa tgccatcaaa
      241 tacgcttatg aactctttaa cggtgtcaca ggccaacgcc tctttccaat tggtagcgac
      301 tgtgttcgtc attttcatcg attgtccctt gaccagcaat tggaagagga agaaaaactg
      361 ctcagaaagg ttgaaaatct aaccagaaaa gctcagaaaa aggaaaaaat caaggtcaat
      421 aaaagtgact ttgacgagcg acttctaaaa tggctctggg aaaaaggtgt tttcaaaccc
      481 aatcgtggca atcaatttgc gcctgagaga gactaccagc ttttcctaga agtctttcag
      541 ggaggaagtt ggaccaaggc ggaaccaaag aaaaaggctc ggatggaaga agtccttgaa
      601 aagtgtatca aacccttttt acttgggaaa tcggatgacc aactctacct tgtcaagcta
      661 ggcaaggaga aaattgacta cgaacaagaa ctccgtatcc aggcagagaa agaacgcaag
      721 aagagggata aaatcgccaa gcaatatgca gataatctgg ttctagccat gggacccgca
      781 gagcgtgctt atcaagatta ctttggcttt acagagaccc tgactcaaga agaacgcaag
      841 tgggaaaaaa ttctttttgg taaaaataga gcagaacggg ctatcaaggc caaacaatac
      901 caaaaagagc tggaaaaaga ccaacgaatt gccagtcagg atccaattga aagaaaacag
      961 aagcagactt ggctcctcaa ttcctatttt cgagagcttc ctgaagaaaa agctcgattt
     1021 tctaggctct tattagaata tcgaaaaagt ggtgaagtac ccttttcaac tgaatacctg
     1081 tcagatcatc tcatcgactt tttctataaa atgaaagcct ttgaatttga aatcgcacca
     1141 gaacaagtcc gagattttct aaaaaaaagc ctgcaggaag accatcgctc atcagcacaa
     1201 ggaagctgga tagaaggaat tctcctcaac tgcctcaaac catttttagg acgattagtg
     1261 atatgagtaa cacctaccgt gtaaatttca tggtaggtgt tattgtaccc caaaataata
     1321 taaaacaaag tatgaaagcg catctcaatt ttaagacttt tttgtaaatt aagagtataa
     1381 ttaa
//
LOCUS       DAVKWS010000132         1320 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKWS010000132 DAVKWS010000000
VERSION     DAVKWS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.133"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            12..806
                     /locus_tag="VW852_002124"
     CDS             12..806
                     /locus_tag="VW852_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0273173.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT          548 a          200 c          211 g          361 t
ORIGIN      
        1 aggaatatat aatgaaaggt ctgtggtcaa ataatcttac ctgcggttat gatgaaaaaa
       61 taatcttgga aaatataaat ataaaaatac ctgaagaaaa aatatcagtt attattgggt
      121 caaatggttg tgggaaatca acactcatta aaaccttgtc tcgacttata aagccattag
      181 agggagaagt attgcttgat aataaatcaa ttaattctta taaagaaaaa gatttagcaa
      241 aacacatagc tatattacct caatctccaa taatccctga atcaataaca gtagctgatc
      301 ttgtaagccg tggtcgtttc ccctacagaa agccttttaa gagtcttgga aaagatgacc
      361 ttgaaataat aaacagatca atggttaagg ccaatgttga agatctagca aataacctag
      421 ttgaagaact ttctgggggt caaaggcaaa gagtatggat agctctagcc ctagcccaag
      481 atacaagtat cctactttta gatgagccaa ctacttactt ggatatctca tatcaaatag
      541 aactattaga cctcttgact gatctaaacc aaaaatataa gacaaccatt tgcatgattt
      601 tgcacgatat aaatctaaca gcaagatacg ctgattacct atttgcaatt aaagaaggta
      661 aacttgttgc agagggaaag cctgaagata tactaaatga taaactagtt aaagatatct
      721 ttaatcttga agcaaaaatt atacgtgacc ctatttccaa ttcgcctcta atgattccta
      781 ttggcaagca ccatgttaac tcttaataat tgaatattgg aattcattta aaagtcgcaa
      841 attttgaact gaccctcaaa agttaggcca aaaaactaac taaaggaggt cagtttttta
      901 atggcaaata cagcacagaa tttaagataa aattagtaaa agaatatcat gaatgaaaaa
      961 taggatatag agaataacag caatattaaa aagatcagga ctgataataa acaaaaagaa
     1021 agtacaacga ctagttcaaa agctaaaact tcaagtaaaa agttattcaa gaaaatctag
     1081 aaaatactca tcctacaaag gacaggtagg aaaaatatca gacaacaaaa taaaaagaaa
     1141 tatttattac cgaaaaaaag ggaaaatcga gtcaagtacc aacaggctca tctattccaa
     1201 aaagatattt actcgatgga aaaactccta gaattagtgc tacaaatatt gacaatggta
     1261 tcttaggtta ctacgaagat atagatgata aaaattatag agtttttgaa aattttatat
//
LOCUS       DAVKWS010000133         1180 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKWS010000133 DAVKWS010000000
VERSION     DAVKWS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.134"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..1063
                     /locus_tag="VW852_002125"
     CDS             <1..1063
                     /locus_tag="VW852_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0273174.1"
                     /translation="LSEKYKQISGKNIKIIEKRYDELLEMIESGDVSSSFDMIRMDMA
                     WLPTFGKKYFQEITSFRQTKSINQNISKSVSREYMYIDQKQYTFPLDVSSQILVYRKD
                     LFEDSFLQRRYFEKTKRKLTVPKSYQEFDELSEFFTLTENPFSPTLYGHSLALSSSVR
                     AACEFIPRFRERLAQSRMNLAVLPQVLTEYEKSYQFTEKSFNSSWNDFVTNLNSGKTS
                     MEIIFSNYTSPLFDGLNVSAQFEFATATIPGNTPVIGGGSIGISKYSNRVEECLNFIN
                     WLYSEEISILLTSLGGFLPSKYVMQNRMLQFQYPWLSSLEDTFSIGSRTKWYGFNTDF
                     RFEQMLGQELIESVKHRYG"
BASE COUNT          409 a          143 c          204 g          424 t
ORIGIN      
        1 tttatcagaa aagtataaac aaatttctgg gaaaaatatt aaaattatcg agaaaagata
       61 tgatgaactt ttggagatga ttgagtctgg cgatgtttca tccagttttg atatgattag
      121 aatggatatg gcttggcttc caacatttgg gaaaaagtat tttcaagaga ttacaagctt
      181 tagacaaaca aaaagtatta atcaaaatat ttctaagtcg gtgtctcgag aatatatgta
      241 tattgatcag aagcagtata cttttccatt ggatgttagt agtcagattt tagtatatag
      301 aaaagactta tttgaagatt catttttaca aagacgatac tttgaaaaaa caaaaagaaa
      361 attaactgta ccaaaaagtt atcaagagtt tgatgagtta agtgaatttt ttacattaac
      421 agaaaatcca ttttcaccta cactttatgg ccatagttta gctttaagtt cttcagtgag
      481 agcagcatgt gaatttattc ctcggtttcg tgagagatta gcacaatcta gaatgaatct
      541 tgctgtactt cctcaggttt taactgagta tgagaaaagt tatcagttta ccgaaaaatc
      601 atttaattct tcgtggaacg atttcgttac gaacttaaac agtggtaaaa cttcaatgga
      661 aataattttt tcaaattata ctagtccatt atttgatggt ctcaatgttt ctgctcagtt
      721 tgagtttgca actgccacta tacctggtaa tactcctgtg ataggtggtg gctcaattgg
      781 aatttctaaa tattcaaata gagtggaaga atgtctaaac tttattaatt ggttatattc
      841 tgaagagatt tctattttac taacatctct gggtggtttt ttaccatcaa agtatgtcat
      901 gcaaaatagg atgttacaat ttcaataccc atggttatct agtttagaag atacatttag
      961 tattgggagt agaacaaaat ggtacggctt taatactgat tttagatttg agcagatgtt
     1021 agggcaagag ttaatagaaa gtgtgaagca tagatatgga taaattaata ctaaattttt
     1081 attactagtt tatttattat tgtttaaaat taaagattca agtaaacacc aattatattt
     1141 aaaattatta ttttgacatt tttctattgc aaagttattt
//
LOCUS       DAVKWS010000134         1176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKWS010000134 DAVKWS010000000
VERSION     DAVKWS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.135"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..384
                     /locus_tag="VW852_002126"
     CDS             <1..384
                     /locus_tag="VW852_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273175.1"
                     /translation="SAINYYRLGMNFEDRNIYCARNYCANLLKIALVEKDVEVLKEFY
                     YTSVYTAKTILGSLEKIHRKSSEYDDIWFLSNQEDLMLISGLLSKPINDIEGLTERQK
                     KTINEGSIMLSEDLQRIKTAIVNGN"
     gene            complement(381..953)
                     /locus_tag="VW852_002127"
     CDS             complement(381..953)
                     /locus_tag="VW852_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273176.1"
                     /translation="MTNKKIRVFLSRPNPFTENQNLFIKELIKFLRRHNIECVTLQAA
                     EYTPYEVMNSLSEMIQRSYGIIIVAFGQTFISSGTRKMGAEDNPDFFASKETHLKNKW
                     VTSVYCHIEGILALTYNLPMLSIPQESLTEEGILKKGEYSITSPEFSLETKENILMYL
                     QSEGFQKSFHVWKNLLDDKYNFIKGGEINY"
     gene            1047..>1176
                     /locus_tag="VW852_002128"
     CDS             1047..>1176
                     /locus_tag="VW852_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0273177.1"
                     /translation="MDYKLISTYLDYCKTHKRLSSHTIRAYKNDLMQFYNSNYDNVE"
BASE COUNT          395 a          181 c          172 g          428 t
ORIGIN      
        1 tctgcaatta attactatag gttaggcatg aattttgaag atagaaatat ttattgtgca
       61 aggaattact gtgctaacct gttaaaaata gccttagttg agaaagatgt tgaagtttta
      121 aaggagttct actatacatc tgtttataca gcaaaaacta ttttaggatc tttagaaaaa
      181 atacatcgta aatctagtga atatgacgac atttggtttc tttccaatca agaggatcta
      241 atgctgattt caggccttct ttcaaaacct attaatgata ttgagggttt aactgaacga
      301 cagaaaaaaa caattaatga aggtagtatt atgttaagtg aggatctcca gaggattaaa
      361 actgcaattg taaatggtaa ttaatagttt atttcccctc ctttgataaa gttatactta
      421 tcatcaagaa ggtttttcca tacatgaaaa cttttttgaa acccttctga ttgcaagtac
      481 attaatatat tttcttttgt ttctaatgag aattcaggtg atgttataga atattctccc
      541 ttctttaaaa tcccttcttc agttaaactt tcttgaggaa tagatagcat tggaagatta
      601 taagttaacg ctaaaatccc ttcaatatga caataaacac ttgtaaccca cttattcttt
      661 aagtgggtct ctttggatgc aaagaagtct ggattatcct cagctcccat ttttctggtt
      721 ccactagaaa taaatgtctg accaaatgca acaattataa ttccgtaact cctttgtatc
      781 atctcactca acgaattcat gacttcgtaa ggagtatact cagcagcttg taatgttaca
      841 cattcaatat tgtgtcgtct taaaaattta atcaattctt taataaataa attttgattc
      901 tctgtaaatg gattaggtct gcttaaaaat actcttattt ttttatttgt catattcgct
      961 atctcctacc gataacaatt tattatcact atacaaagca ctcaaatagg tttataatag
     1021 catgattatt gataaaggag atttttatgg attataagct tatttctact tacttagatt
     1081 attgcaaaac tcataagcgt ttgagttcac acacgattcg agcttataag aatgatctta
     1141 tgcaatttta taattcaaac tatgataatg tcgaat
//
LOCUS       DAVKWS010000135         1084 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKWS010000135 DAVKWS010000000
VERSION     DAVKWS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.136"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(717..977)
                     /locus_tag="VW852_002129"
     CDS             complement(717..977)
                     /locus_tag="VW852_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273178.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
BASE COUNT          364 a          206 c          179 g          335 t
ORIGIN      
        1 tatgcacaga catatgaaat ctttttgaga cagcagttat gatatattta tccattcaca
       61 acatcttctg tactaatact acaatattgg tacattttga ttacacaatc gtttcaaaaa
      121 atatgatgga agcctgagta ttttatagta attcccttcc tatcaactag atcaaccagt
      181 tgagtgttga atgttgaaac accctagcct cagcaaagga ctatgatcag catttcctag
      241 ccaataggac gctccaaatc acctattcta agtccatcac tagcttggtc agtaagatac
      301 agactgcaag gcgagactga tgtggttgat gagattttgg aagagtatta caatcataga
      361 ttcaccagta gacttgaccg cagatttaac tatagtagat tgaaactaga atagtacacc
      421 tctacttcta aaacattgtt agaaatcgat ttgactgtcc tgatcaattt gtcctgttct
      481 cgtttcattt tactatacta aaaaaccacc tgaaacaaat caagtgacta gaggataaaa
      541 acaaaaactg ggcaaaaagt tttgaaaagc aaaaaacgca taatatcagg tcttgaaaaa
      601 ccttgatact atacgtttga ttgtgggaag attgacttca ttttctcctg aaattgaagt
      661 tttttctagt ttccagatat agacgaattc aatatagcca tctcgctaca attattttaa
      721 ccatcgagaa aatttttcag aatttcaaaa atttcatttt gatccaatga aatgtcatta
      781 ttttgaagaa tagttgactt cacaggctct aattctatca caaagcggtg catgtctgct
      841 tttaccgcac tacttgatgt tatagccgat aacttttcat ctcctagcaa ggaagctgtc
      901 aaacgggtaa ggtcattcaa atgcttttta atggacttac ttaaaccttg agcacctgtg
      961 gcagagcgct ccctcatatc cagccaagct ttagccttaa aaataattaa tactcaatga
     1021 aaatcaaaga gcaaactagg aagctagctg caggctgctc aaaacactgt tttgaggttg
     1081 taga
//
LOCUS       DAVKWS010000136         1063 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKWS010000136 DAVKWS010000000
VERSION     DAVKWS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.137"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VW852_002130"
     rRNA            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VW852_002130"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(173..>1063)
                     /locus_tag="VW852_002131"
     rRNA            complement(173..>1063)
                     /locus_tag="VW852_002131"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          246 a          312 c          223 g          282 t
ORIGIN      
        1 cacagggggc aacccccaac tacttccggc gttctagggc ttaacttctg tgttcggcat
       61 gggtacaggt gtatctccta ggctatcgtc acttaactct gagtaatacc tactcaaaat
      121 tgaatatcta ttcaatttaa gaaaaccgtt cgctttcata ttctcagtta ctttggataa
      181 gtcctcgagc tattagtatt agtccgctac atgtgtcgcc acacttccac ttctaaccta
      241 tctacctgat catctctcag ggctcttact gatatataat catgggaaat ctcatcttga
      301 ggtgggtttc acacttagat gctttcagcg tttatccctt ccctacatag ctacccagcg
      361 atgcctttgg caagacaact ggtacaccag cggtaagtcc actctggtcc tctcgtacta
      421 ggagcagatc ctctcaaatt tcctacgccc gcgacggata gggaccgaac tgtctcacga
      481 cgttctgaac ccagctcgcg tgccgcttta atgggcgaac agcccaaccc ttgggaccga
      541 ctacagcccc aggatgcgac gagccgacat cgaggtgcca aacctccccg tcgatgtgaa
      601 ctcttggggg agataagcct gttatcccca gggtagcttt tatccgttga gcgatggccc
      661 ttccatacgg aaccaccgga tcactaagcc cgactttcgt ccctgctcga gttgtagctc
      721 tcgcagtcaa gctcccttat acctttacac tctgcgaatg atttccaacc attctgaggg
      781 aacctttggg cgcctccgtt accttttagg aggcgaccgc cccagtcaaa ctgcccgtca
      841 gacactgtct ccgataggga tcacctatct gggttagagt ggccataaca caagggtagt
      901 atcccaacag cgtctccttc gaaactggcg tcccgatctc ttagactcct acctatcctg
      961 tacatgtggt acagacactc aatatcaaac tgcagtaaag ctccatgggg tctttccgtc
     1021 ctgtcgcggg taacctgcat cttcacaggt actaaaattt cac
//
LOCUS       DAVKWS010000137         1053 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKWS010000137 DAVKWS010000000
VERSION     DAVKWS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.139"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            79..261
                     /locus_tag="VW852_002132"
     CDS             79..261
                     /locus_tag="VW852_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV0273179.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            271..633
                     /locus_tag="VW852_002133"
     CDS             271..633
                     /locus_tag="VW852_002133"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV0273180.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYGDSAWEHLRAVTGID
                     LLSILTDIANKKTGVKSK"
     gene            630..>1053
                     /locus_tag="VW852_002134"
     CDS             630..>1053
                     /locus_tag="VW852_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV0273181.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNT"
BASE COUNT          435 a          130 c          171 g          317 t
ORIGIN      
        1 tagaaagaaa aaatcgaaaa attagctaag aaatattctg ataacttaaa catcaaagtt
       61 caagagagag ttcgtgaaat ggcaaatgat aataagagcc attatttgat atacagagtt
      121 ttaggtattt catttgaaga aggagaaaat atcgatttgt atcaaaataa aggtcgtttt
      181 ttatacaaat atgctggttc atttttagaa gaagctgcag tactatcctt taacgaaaaa
      241 tttggtacag aaaatactta aaaagttaac attcctaatt ctgaaagtac aaaacctaag
      301 acttttgaaa ttgattgttt agtcggagaa aaacacgcat acgaaataaa atggtgggat
      361 gcaactacag atggagacca tataactaaa gaacacacta gaataaaagt tattcataac
      421 aaaggatata taccaattcg gttaatgttc tactatccaa atagaactca agctataaaa
      481 attcagcaaa ctttagaaac attgtataac ggtattggag ggaaatatta tggagattct
      541 gcctgggaac atttaagagc agtgaccggt attgatttac ttagtattct aacagatatt
      601 gcaaataaaa aaacaggggt aaaatcaaaa tgacagtatt aaaaggagat aacttagaaa
      661 tattaaaaac tattgaatcc tcaagtattg atttaatcta tatggaccct cctttcttta
      721 cacagaaaac ccaaaaatta tctaataaca aaaatattat gtattcattc gaagatacgt
      781 ggacttcgat tgaggattac aaagaatttt tgtctgtaag attagaagaa tgcaaaagag
      841 tgctaaaaaa tagtggcagt attttcgttc attgtgataa aattgcaaat catcatatta
      901 gattaatttt agataatatc tttggagcag atatgtttca aagcgaaatt atatggaact
      961 ataagcggtg gtctaattca aaaaagggat tattgaacaa tcatcaaaac atttactttt
     1021 attcaaagtc aaaagatttt aaatttaata caa
//
LOCUS       DAVKWS010000138          978 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKWS010000138 DAVKWS010000000
VERSION     DAVKWS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.140"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            196..>978
                     /locus_tag="VW852_002135"
     CDS             196..>978
                     /locus_tag="VW852_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273182.1"
                     /translation="MTSCFVVMGFNTKKIPNTNIEVDLNQVYNNLIKPTILEKELVSV
                     HGKNHFRADEVFSTQSITKTFIEGILKADIVIADITTLNQNAIYELGLRHAMKPKSTI
                     ILCDHHTAKISFFDIAHLPQIRYDSDKLNEVDEVNKVKKLLSEYIDSAIKSDETFTDS
                     PAFESALYRVIINDLIDKTEIQSEEALDKSIAELYDQATELKNSEKYPEAEEIFQQIL
                     ESGYIDEEILAGYLLSSYKKNESSLANLKMAQQNISKYIDINT"
BASE COUNT          384 a          138 c          154 g          302 t
ORIGIN      
        1 acacaattct cgagttgttg taagtttgta attgactcaa aagtattaga tataatttca
       61 aaacagtatt ttactttata gttgaccaat aacatttaat tctaactaaa aaaaccatta
      121 tacaaatcaa gctactaaaa atgactttat ggtatactag tgataataaa ttgttatcgg
      181 gaggaaagtt gagaaatgac cagttgtttt gtagttatgg ggtttaatac taaaaaaatt
      241 cctaatacaa atatagaagt ggacttgaat caagtctata ataatctaat taagccaaca
      301 attttagaaa aagaattagt ttctgttcat ggtaaaaacc actttagagc tgatgaagtt
      361 ttcagtacac agagtataac gaaaacattt atcgaaggta tattaaaagc agatattgta
      421 attgcagata ttactacttt aaaccaaaat gctatctatg agttaggtct acgtcatgct
      481 atgaaaccca aatcaaccat tatactgtgt gatcatcaca cagctaagat tagctttttt
      541 gatattgcac acttacctca aatcagatat gattctgata agttaaacga agtggatgaa
      601 gttaacaagg tgaaaaaact gctttctgaa tatattgatt ccgctataaa gtcagatgag
      661 acatttactg atagtccagc ctttgaatct gctctatacc gtgtaataat taacgattta
      721 atcgataaaa ctgaaattca aagtgaagaa gcattagaca agtcaatagc tgaactttat
      781 gatcaagcca cggaactcaa gaattcagaa aagtatcctg aggcagaaga aatatttcag
      841 caaattttag aaagtggata tattgatgaa gaaattttgg caggctattt actatcatcc
      901 tataagaaaa atgaatcttc gctagcaaat ttaaaaatgg cacagcaaaa tattagtaaa
      961 tatatagata taaacacc
//
LOCUS       DAVKWS010000139          907 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKWS010000139 DAVKWS010000000
VERSION     DAVKWS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.142"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            1..907
                     /locus_tag="VW852_002136"
                     /pseudo
     CDS             1..907
                     /locus_tag="VW852_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          336 a          158 c          180 g          233 t
ORIGIN      
        1 aaaaagagac ctgaagagag gacagaatta gaacatcttc aagcagaaaa tgagtacctg
       61 agagcggaga atgccatcct aaaaaagtta agagaactcc gattgaagga ggaaaaagag
      121 aaagaagaaa gacagaaatt gttcaagaat taatgactga gttttcgtta gatattcttc
      181 taaaagccat taaactagct cgttcgacct actactatca cttgaaacag ctagacaaac
      241 cagataagga ccaagagctt aaagctgaaa ttcaatccat ttttatcgaa cacaagggaa
      301 attatgctta tcgtcggatt tatttagaac taagaaatcg tggttatctg gtaaatcata
      361 aaagagttca aggcttgatg aaagtactca atttacaagc taaaatgcga cagaaacgaa
      421 aatattcttc tcataaagga gacgttggta agaaagcaga gaatctcatt caaggccaat
      481 ttgaaggctc taaaacaatg gaacagtgct acacagatgt gactgaattt accattccag
      541 taagtactca aaagctttac ttatcaccag ttttagatgg ttttaacagc gaaattatcg
      601 cctataatct ttcaacttca cccaacttag aacaagtaca aacaatgttg gaacaggcat
      661 tcacagagaa gcactacgag aatacgattc tctatagtga ccaaggctgg caatatcaac
      721 acgattctta tcatcagttt cttgagggta agggaattca agcatccatg tcacgtaagg
      781 gtaacagccc agacaatggt atgatggagt ccttctttgg gattctgaaa tcggaaatgt
      841 tttacggtta tgagaagtcg tttcagtcgc ttaagcaatt ggaacaagcc attgtagact
      901 atattga
//
LOCUS       DAVKWS010000140          878 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKWS010000140 DAVKWS010000000
VERSION     DAVKWS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.143"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            681..842
                     /locus_tag="VW852_002137"
     CDS             681..842
                     /locus_tag="VW852_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273183.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          347 a          114 c          165 g          252 t
ORIGIN      
        1 aaaagaggtg gaaaggcact attaaagact ttaagtttaa aaaggtatca aagtatgcca
       61 gagataaaaa aatataaata tgacacttta aaggcgatta gaaaattaaa gaaatgctag
      121 aagactttta gatgacagtt accaagaggg tctaattttc actgctactt atgcagtagg
      181 aattggacct ttttatataa gcaaattcta tttcaaaatt ggttaccaag aaagacttct
      241 tttcactgtt aagtataaga gggtaattta tagaattttg ttacatcaag ataaatattt
      301 tctgatggtc aaattttaaa aagattaata actctcgata aattttgaac cttaacaatt
      361 gaatagacca agacaagacg ttaattattt ttggagcgta ataacctatc cgtcaagatc
      421 tttcggctga ataattcggc aaaaaaagtt ctgctaagca aaaagaaagt gagcgataag
      481 taagagtttg aatataggga gggataccct atccgctatg aagaaaatga ggataatgat
      541 acttctaagg ttgaagcagg ctcatccgga atagaggcgg aggtgagatt cctatgttgc
      601 taatccaaag cacttgtcaa tgtcaaaaaa cttaattaaa caaataatta ttagatataa
      661 aactttagag gaggtatata ttggacaatg actggaatgg attggctgat ttaatagcaa
      721 atctgattgc aaaatatgct ggtgctttag atttagataa tctacccgat ccaacgccag
      781 caaaaaatca agagatgaaa aatagttttg acatggcaaa aacacaaatt gagacggact
      841 aataaaagtg ttataatata cttgaaatga tagtccaa
//
LOCUS       DAVKWS010000141          877 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKWS010000141 DAVKWS010000000
VERSION     DAVKWS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.144"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            116..877
                     /locus_tag="VW852_002138"
                     /pseudo
     CDS             116..877
                     /locus_tag="VW852_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          332 a           97 c          161 g          287 t
ORIGIN      
        1 gagcaaacca tgaatattaa tggaggaata aaaaatgtaa gttataaatg taagtaagca
       61 ttatggtcat tcaatcattc tcaaagatat aaattttgca cttaacaagg gtgaaattgt
      121 tggtctagta gggagaaatg gagttggtaa gagtacgttg atgaaaattc ttgttcaaaa
      181 taatcaaccg acttcaggta atattataag cagtgataat gttgggtatt taatcgaaga
      241 accaaaatta tttttatcta aaacaggttt agagaattta aaatatttgt caaatttata
      301 tggtgttgac tacaatcaag aaagatttag aagtttgatc caagagttag atttgactca
      361 gtctattaat aaaaaagtaa agacctattc tttgggtaca aaacaaaaat tagctttgct
      421 tctaactctc gttacggaac ctgatatatt gattttagat gaaccgacta atggtttaga
      481 tattgaatca tcacaaatag ttttagcggt tctaaaaaaa attagcttta aatgaaaacg
      541 tgggaatttt aatatcgagt cataaattag aagacattga agaaatttgt gagagagttc
      601 ttttcttgga gaacgggctt ttgacatttc aaaaagtagg aaaagatagt cataatttct
      661 tgtttgagat agctttttca tcagctacag atagagacat tttcattacc aaacaagaat
      721 ttggagatat tgttcaggaa gagggactga gaattactat gtctgggaat attcaaagta
      781 gtgagctttt taaatttttt aacgaaaact ctattaaagt agttgatttt gaaactaaaa
      841 aagagacgct taaagatatt tacctaaatc gttcaaa
//
LOCUS       DAVKWS010000142          791 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKWS010000142 DAVKWS010000000
VERSION     DAVKWS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.146"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..>791
                     /gene="phtD"
                     /locus_tag="VW852_002139"
     CDS             <1..>791
                     /gene="phtD"
                     /locus_tag="VW852_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV0273184.1"
                     /translation="SRPEQPSPQPTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKV
                     GDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYN
                     KAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPE
                     RLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIK
                     KDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRV"
BASE COUNT          263 a          170 c          172 g          186 t
ORIGIN      
        1 tcaagaccag aacaaccaag tccacaaccg actccagaac ctagtccaag tccgcaacct
       61 gcaccaaatc ctcaaccagc tccaagcaat ccaattgatg agaaattggt caaagaagct
      121 gttcgaaaag taggcgatgg ttatgtcttt gaggagaatg gagtttctcg ttatatccca
      181 gccaaggatc tttcagcaga aacagcagca ggcattgata gcaaactggc caagcaggaa
      241 agtttatctc ataagctagg agctaagaaa actgacctcc catctagtga tcgagaattt
      301 tacaataagg cttatgactt actagcaaga attcaccaag atttacttga taataaaggt
      361 cgacaagttg attttgaggc tttggataac ctgttggaac gactcaagga tgtctcaagt
      421 gataaagtca agttagtgga tgatattctt gccttcttag ctccgattcg tcatccagaa
      481 cgtttaggaa aaccaaatgc gcaaattacc tacactgatg atgagattca agtagccaag
      541 ttggcaggca agtacacaac agaagacggt tatatctttg atcctcgtga tataaccagt
      601 gatgaggggg atgcctatgt aactccacat atgacccata gccactggat taaaaaagat
      661 agtttgtctg aagctgaaag agcggcagcc caggcttatg ctaaagagaa aggtttgacc
      721 cctccttcga cagaccatca ggattcagga aatactgagg caaaaggagc agaagctatc
      781 tacaaccgcg t
//
LOCUS       DAVKWS010000143          788 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKWS010000143 DAVKWS010000000
VERSION     DAVKWS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.147"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(21..92)
                     /locus_tag="VW852_002140"
     CDS             complement(21..92)
                     /locus_tag="VW852_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEV0273185.1"
                     /translation="MKKISKFFPILMLVMDIIIIVGG"
     gene            174..>788
                     /locus_tag="VW852_002141"
     CDS             174..>788
                     /locus_tag="VW852_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEV0273186.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTKTESFANLIQYIRKQYSLQNINNIQSLLSDS
                     SNYTLDSFEKTMVKSILHTMDSRIIPSDDELLQLADYLFKVEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKRIVTQLAINCFILSIDKE"
BASE COUNT          296 a          129 c          103 g          260 t
ORIGIN      
        1 taggtgtaca cctaagtgat ttaaccacca acaataatga taatgtccat cactagcatc
       61 aaaattggga aaaattttga aattttcttc atatttagac ttcctttcaa tttttattta
      121 ttgtacaata aataaaacta agttaacttt ttgttgggag aatgggaaaa actatgaaat
      181 caaaacttgg catcacactt agaaaagttc gtaaaggcaa acaaatcagt ctatgctctg
      241 ttgctgatga gcatctttca aaatcgcaaa tatcccgatt tgaacgtgga gagtcagaga
      301 tatcatgtat ccgactgatc aatattttag acaaattaca cattactttg gatgagtttc
      361 ttattttaca cgatgaagac tatacaaaaa ctgagtcatt tgccaactta atacagtata
      421 tccgtaaaca atattcatta caaaacatca ataatataca atctctactt tcggattcct
      481 caaattacac tttagattct tttgaaaaaa caatggtaaa atccatttta cacacaatgg
      541 attcaagaat cattccatca gatgatgaat tacttcaact agcagattat ctctttaaag
      601 ttgaaaaatg gggttattat gagattattt tattaggtaa ctgtgtacga acaattaact
      661 acaactccta cttcttatta actaaagaaa tgttaaataa ttatatttac tcatctctaa
      721 ataaaacaaa taagcggatt gtcactcagc tagctataaa ttgtttcatt ctcagcatag
      781 ataaagaa
//
LOCUS       DAVKWS010000144          769 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKWS010000144 DAVKWS010000000
VERSION     DAVKWS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.148"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(83..>769)
                     /locus_tag="VW852_002142"
     CDS             complement(83..>769)
                     /locus_tag="VW852_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV0273187.1"
                     /translation="LDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPK
                     GYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPQTEKPDE
                     DKGHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVE
                     HSVINAKIADAEALLEKVTDPSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLAL
                     LKESQPAPIQ"
BASE COUNT          199 a          157 c          139 g          274 t
ORIGIN      
        1 gtcaagtttc tgttacgttt ttctaaaaaa gagaagtaac acgaaaggtt acttctccat
       61 aaaatatgct ccattcattt tactactgta taggagccgg ttgactttct tttaacaaag
      121 ccaagagact atctacttct gctgaaatag tgttattatc tttcgttccg agaagaagac
      181 tactttttag accagtcaat gtctccatag cattttgtct aatactagga tctgttactt
      241 tttctagcaa ggcctccgca tctgctatct tagcgttaat aacagaatgc tctacttgag
      301 gaatttcagc ctcatctgta gtatcttcag cttcttcctc tgtctcttcc gtatcagtgc
      361 ttggtttata aagattatct gctgaaggat ttaaaccagc gtgattctct ttttcatcag
      421 attcagggtg agttggctca cttacttcat catgtccctt atcttcatca ggtttttctg
      481 tctgaggttt ctccgtttga ttggtatcag cttgaccatt tttgtttctt tgaacatggt
      541 cgctagcgtt accaaaacca ttatctgaat gcggacgttc gtttggatgt tcgacatagt
      601 acttgacagt cgccaaaaga tcctcaagag tatacccctt aggtgcctca taaaggcctt
      661 cgtcaaacca ctcaaatttg atgttatggt aatggtcata atgaggtatg attaaactac
      721 cgtttttgac ttctacagta tattgaagat tgtaaggcat acgatcaag
//
LOCUS       DAVKWS010000145          675 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKWS010000145 DAVKWS010000000
VERSION     DAVKWS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.149"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(55..>675)
                     /locus_tag="VW852_002143"
     CDS             complement(55..>675)
                     /locus_tag="VW852_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV0273188.1"
                     /translation="MPFYETNPLKASNPYGRTKQYIEVLLNDLFISNSNWKIVCLRYF
                     NPLGAHESGDLGEDPNGIPNNLVPYITQVAIGKLPYLNIFGVDYSTPDGTCIRDYVHV
                     NDLAYGHRKALEYIFNTDEGLYEVINLGSGVGFSVFEILHSLESVIGSYIPYKITSRR
                     AGDMDVSIADISKAEELLGWKPRYDIMKMCQDTWKWQQKHPNGYDD"
BASE COUNT          209 a          142 c           87 g          237 t
ORIGIN      
        1 gtgcagaata tctaaataat actatatgca tgttaatccc aatcctttca attattagtc
       61 atcatatcca tttggatgtt tctgttgcca tttccacgta tcttggcaca ttttcataat
      121 atcatacctt ggcttccatc ccagtagctc ttctgctttt gaaatatctg caattgaaac
      181 gtccatatct cctgctctcc ttgaagttat cttatatgga atatacgagc ctatgacact
      241 ctctaagctg tgtaggattt caaatacact aaaaccaaca ccacttccta aattgattac
      301 ttcatataaa ccttcatctg tattaaaaat atactctaat gcttttctgt gtccgtatgc
      361 taaatcgtta acgtgaacat agtcccttat acaagtccca tcaggagtac tgtaatcaac
      421 tccgaatata tttaaatatg gtaattttcc aattgcaact tgtgtaatgt aaggaacaag
      481 attattcgga attccatttg gatcctctcc taaatcacca ctttcatgtg cacccaatgg
      541 attaaaatac ctcagacaaa caattttcca atttgaattc gaaataaaca aatcatttaa
      601 aagtacttct atatactgtt ttgttctccc atacgggttt gaagctttta atggattagt
      661 ctcatagaat ggcat
//
LOCUS       DAVKWS010000146          650 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKWS010000146 DAVKWS010000000
VERSION     DAVKWS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.150"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            53..415
                     /locus_tag="VW852_002144"
     CDS             53..415
                     /locus_tag="VW852_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV0273189.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSISLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          216 a           81 c          121 g          232 t
ORIGIN      
        1 acctgaggct agtttcctag tttgctcttt ggttttcatt gagtataaca cattgttaga
       61 agttggttta aattccctaa tcagtttgtt cacatttacc ttcgatatat tatatcccat
      121 agttaaggtt ggtcatacag atgattatag ccatggagcc gtaaaactta gtatttcttt
      181 agttgacaaa gatgccatga aaaaaatatc tgtaactgta ataggatatt ttgaaataaa
      241 tatagatgaa aatatcaccg atattctata cgtaaatggt actgctattc tttatcctta
      301 tttacgttct attgtttcaa tagtttcggc aattgatagc agtgaagcaa tgttgctacc
      361 taccattaat gttttagagt tactagataa atctcaacct tttgaagaag aataatttat
      421 tagctcacta aattgagggt aaggaaaagt aaaagcagta agaaaaatgt cttgcattat
      481 acagcaacct tttgggaatg agtagatgga ttgaataaaa tttgattaag agtggatgat
      541 ttatctgtag attattattg gacagttagt cttgaagtag tctaagaatt aggttataat
      601 cagtagaagc cttgctaata atgaggaggt tagtttatgt atagtagact
//
LOCUS       DAVKWS010000147          637 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKWS010000147 DAVKWS010000000
VERSION     DAVKWS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.151"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..416)
                     /locus_tag="VW852_002145"
     CDS             complement(<1..416)
                     /locus_tag="VW852_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0273190.1"
                     /translation="MVTIKDIARVSGVSHGTVSNVLNKKGNVSAAKIKLVEETAKKLG
                     YNMNSQAQFLRKGASSKCFFLLFNNARKKYAEYFAEIDQLSLDVEYVYLHKFSEIKNK
                     ISAILSENPQFIVCLGFSPKRFVDIEDNIPIFEVDT"
     gene            complement(426..>637)
                     /locus_tag="VW852_002146"
     CDS             complement(426..>637)
                     /locus_tag="VW852_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV0273191.1"
                     /translation="YVISCEVKMMKPQKEIYLSLVNKYQLDIKDCIFLDDLEENVEAA
                     RTLGIKAFQIKERKEISNILKDLLN"
BASE COUNT          215 a          107 c           70 g          245 t
ORIGIN      
        1 tatgtatcta cttcaaaaat cggaatatta tcttcaatat caacgaatct ttttggagaa
       61 aaacctaagc aaactataaa ttgaggattt tcacttaaaa ttgcagatat cttattttta
      121 atctctgaga atttatgtaa gtaaacatat tctacatcta aagataactg atcaatttct
      181 gcaaaatact cagcatactt tttgcgtgca ttattaaata acaagaaaaa acatttacta
      241 gaagcgcctt ttcgaagaaa ctgagcttga gaattcatat tataacccaa ttttttcgca
      301 gtctcttcta ctaactttat tttagctgct gagacattac cttttttatt taaaacgttt
      361 gaaactgtcc catgtgagac tccagacact cttgcaatat cttttattgt aaccataatt
      421 aacctctagt ttaagagatc ttttagtata tttgagatct ctttacgctc tttaatttga
      481 aatgctttaa tacctagagt tcttgcagct tctacatttt cttctaaatc atctaaaaat
      541 atacaatctt ttatatctaa ttgatattta ttaactaatg acaagtaaat ttctttttgt
      601 ggtttcatca ttttaacttc acaagaaatt acgtaac
//
LOCUS       DAVKWS010000148          616 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKWS010000148 DAVKWS010000000
VERSION     DAVKWS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.152"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
BASE COUNT          205 a           73 c          103 g          235 t
ORIGIN      
        1 agaggtatga tagaagttga tagatgataa aaaatagctg atatagagaa tttagagcgc
       61 ctttggaata tatttttgga atttaatcaa tcacagttga atgtgaaggg caatcctttt
      121 tagaagaata caatcaagat accattatgc tatttcttta gttcagttaa aatattgaga
      181 agtattactt ttattgaaaa agcatcacaa aggactccaa ttaattttta atgatttttc
      241 taatctaatt ttgtggttat tgacaaataa agtctagtta attgctttga aatccatgga
      301 gtggtctata tacatctgtt aatgcaacgg aaaattttat tgataagtag ggggtggcta
      361 ttattcaact tttaggttct gaaagtaaga gattcacatt agaaatatca tgttgaccat
      421 ttttcaattt gctggttagt agtaatctgg acatcattaa tcccttcctt aacaaaatta
      481 tgtgactttt gttaccggat tactgctgat tttttaatag cttataacat ctttagacgg
      541 ttaatagatt ttgaccgtct ttcttaatat tgtcttacct ataatagata tgcttatgag
      601 atatgtttaa taaatt
//
LOCUS       DAVKWS010000149          605 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKWS010000149 DAVKWS010000000
VERSION     DAVKWS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.153"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
BASE COUNT          228 a          112 c           69 g          196 t
ORIGIN      
        1 tattagattt attttagtat atccaatcac tagtccaata attggactag cagacatgct
       61 tagatataat aaaaataaca aaaaatgaat ttcaccaatt cctgcaaaaa aatgtttcat
      121 agctatagtt ttacgcccat aaatacccat caaatttacc gtctacgata tctaaaccat
      181 atgcaatggc tcctcctaat gagaagactt cagcaacagt agccgcaata ttaagagcat
      241 tagtaatagt tgccatacta actccaccaa tatatcttgt tatagcactt agttacctag
      301 ataagatatc ctttagcttg cttccaaaat ttaagtctac acctataata tgagttcata
      361 ttagtactgc taaatcctat ctatagtaat gtcaaacacc taaatcaagt catctctcca
      421 attcatccaa gaaatgaaga aaggcaaaaa atctttaaaa taagaaaagt gcatattatc
      481 aggtgttcaa aaatcttgat aatatgcgtt atataataga aaaatttact caatttcatt
      541 ttcaaattga gttttttccc agcctcattt ttaatttata tcttcaatag aatctaatga
      601 ccgag
//
LOCUS       DAVKWS010000150          593 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKWS010000150 DAVKWS010000000
VERSION     DAVKWS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.154"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..553
                     /locus_tag="VW852_002147"
     CDS             <1..553
                     /locus_tag="VW852_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0273192.1"
                     /translation="YLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNM
                     VSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGD
                     DGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIV
                     THAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          211 a           61 c          126 g          195 t
ORIGIN      
        1 gtatttaatt ttaggtgaaa gtggaagcgg gaaatctaca ttattaaaat tattgaatgg
       61 ctttttgaga gattatagtg gagaattgcg attctgcggg gatgatataa aaaaaacctc
      121 ctatttaaat atggtttcga atgttctata tgtagatcaa aaagcttatt tgtttgaagg
      181 tacgattaga gataatattt tattggaaga aaattatact gatgaagaaa tactacagtc
      241 tttagaacaa gttggtttga gtgtaaaaga ttttcctaat aacattttag attattatgt
      301 tggtgatgat gggagattac tgtcaggagg gcagaaacaa aaaattactt tagctagagg
      361 cctaattaga aataagaaaa tagtattaat tgacgaggga acttctgcta tcgataggag
      421 aacttcgtta gcgattgaac gtaagatatt agatagagag gatttgactg tcattattgt
      481 tacccatgct ccgcatccgg aacttaaaca atattttact aagatatatc aatttccaaa
      541 ggattttatt taagaatata ggggagggaa acatgagatt ctattgtgaa gct
//
LOCUS       DAVKWS010000151          547 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKWS010000151 DAVKWS010000000
VERSION     DAVKWS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.155"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..>547)
                     /locus_tag="VW852_002148"
     CDS             complement(<1..>547)
                     /locus_tag="VW852_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0273193.1"
                     /translation="KVPVFLTKDYKPKKDTALQKGLIYARNGSINTPKDSSAPFELIN
                     KLFQKFNHTDLNIKEQYFHVLKDYKNWFFVENEDGRFFIYNPNPDFYIKLTDDDANRF
                     KTMPYSLNQYQTNVDWQLVQLRYRHLTIIDFMALYLDQGNCLVPSPDLEDFECGYSDT
                     IYYHCLYKNTLKYQLLKVFSSI"
BASE COUNT          194 a           72 c           88 g          193 t
ORIGIN      
        1 atattgaaga gaaaactttc agcaattggt attttaaagt atttttgtat aaacagtggt
       61 aataaatagt gtcagaataa ccacattcaa aatcttctaa atcaggggag ggcactagac
      121 aatttccctg atctaaatat aaagccataa agtcgataat tgtaaggtgt cgataacgaa
      181 gttgtactaa ttgccaatca acattggttt ggtattgatt taaactataa ggcatagttt
      241 tgaaacgatt tgcatcatcg tcggtaagtt taatataaaa atcgggatta ggattataaa
      301 taaaaaatct tccgtcttca ttttcaacga agaaccaatt tttatagtct tttaaaacat
      361 gaaaatactg ctctttgata tttaagtcgg tatgattaaa cttttgaaac aacttattta
      421 ttaactcaaa tggagcagag gagtctttag gagtatttat agagccattt ctagcatata
      481 ttaatccttt ttgtaatgca gtatctttct taggcttata atcttttgtt aaaaatacag
      541 gaacttt
//
LOCUS       DAVKWS010000152          475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKWS010000152 DAVKWS010000000
VERSION     DAVKWS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.157"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..>475
                     /locus_tag="VW852_002149"
     CDS             <1..>475
                     /locus_tag="VW852_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0273194.1"
                     /translation="YCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFRMDSGFATPK
                     LYDLIEKTGQCYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSH
                     KRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKEMKEGFF"
BASE COUNT          132 a          109 c           98 g          136 t
ORIGIN      
        1 tattgtttca atgcccagct tcgtcctggt aatcgttatt gttctgaaga ggcagacagc
       61 tttatcacac ctgttttaga acggtttaat caacttctct ttcgaatgga tagtggcttt
      121 gcgaccccaa aattatacga tttaattgaa aaaacagggc aatgctacct cataaaactc
      181 aagaaaaata ctgttctgag ccgtcttgga gacctttccc tcccttgccc acaggatgag
      241 gacttaacca tcttgcccca ctccgcctac tcagaaactc tctatcaagc aggatcttgg
      301 tcgcacaagc gtcgtgtctg ccagttctct gaacgaaaag aaggaaactt gttctacgat
      361 gttatttctc tcgttacaaa tatgacgagt ggaacaagcc aagaccagtt tcagctttat
      421 cgtggacgtg gtcaagccga gaatttcatc aaggagatga aggagggatt ttttg
//
LOCUS       DAVKWS010000153          464 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKWS010000153 DAVKWS010000000
VERSION     DAVKWS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.158"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            55..>464
                     /locus_tag="VW852_002150"
     CDS             55..>464
                     /locus_tag="VW852_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV0273195.1"
                     /translation="MNPLKECYEKNGILIAAHRGTNGGNILQNTSKAYLNSIRHNADM
                     FEVDVIRSKDGEFFAFHDGQEPLVFGEKIDIRTLSSEEIQRKECFNSLNEPILQKIEK
                     ISDVLKNFEGQSLINIDRSWFYWDTFLDKLKTYPS"
BASE COUNT          186 a           61 c           82 g          135 t
ORIGIN      
        1 cgttccaaga aatcggatac aatgtaatta acaataaaga taggagaaaa taatttgaat
       61 ccattaaaag aatgctatga aaaaaacggt atactaattg cagcacaccg tggtacaaac
      121 ggaggaaata tattacaaaa cacatcaaaa gcctatttga attccatacg ccataacgca
      181 gatatgtttg aggttgatgt aattcgttca aaagatggag aattttttgc atttcacgat
      241 gggcaagaac ctttagtatt tggtgaaaaa attgatatta gaactttgag ttctgaagag
      301 atccaaagaa aggaatgttt taattcactg aatgaaccca tattacaaaa gattgaaaaa
      361 atttctgatg tattaaaaaa ttttgaggga cagtctttaa taaatataga tagaagttgg
      421 ttttactggg ataccttcct agataagtta aaaacatatc catc
//
LOCUS       DAVKWS010000154          453 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKWS010000154 DAVKWS010000000
VERSION     DAVKWS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.159"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            12..230
                     /locus_tag="VW852_002151"
     CDS             12..230
                     /locus_tag="VW852_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0273196.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            234..>453
                     /locus_tag="VW852_002152"
     CDS             234..>453
                     /locus_tag="VW852_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0273197.1"
                     /translation="MTLRTEDQVKDYAREVLGFNEVEENINQGTGQITTFNQLGFKGY
                     SDKPDGWYLPKNMNDVAIILETKSEERDI"
BASE COUNT          190 a           51 c           88 g          124 t
ORIGIN      
        1 agagggatat tatggcactt aactataaac cattatggat acagttagca aaaaaaggac
       61 taaagaaaac agatgtaata gctatggcag gacttacaac aaatgttatg gcacaaatgg
      121 gaaaggataa accaattaca tttaagaatt tagaaagaat atgtaaggct ttatcttgca
      181 ctcctaatga tattattagt tttgaagata attttagtga cgaggaatag aaaatgactt
      241 taaggacaga agatcaagtt aaggattatg caagagaagt attaggcttt aatgaagttg
      301 aagaaaacat caatcaaggt actggtcaaa taactacttt taatcaatta ggcttcaagg
      361 gatattcaga taagccagat ggttggtatt tacctaaaaa tatgaatgat gtagcaataa
      421 tccttgaaac aaaatcagaa gaaagagata tta
//
LOCUS       DAVKWS010000155          371 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKWS010000155 DAVKWS010000000
VERSION     DAVKWS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.162"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            complement(<1..>371)
                     /locus_tag="VW852_002153"
     CDS             complement(<1..>371)
                     /locus_tag="VW852_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV0273198.1"
                     /translation="AKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTTLVAS
                     KILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEENILKLLQA
                     KIVQRNKGIDGFLPKHFQKKP"
BASE COUNT          107 a           63 c           47 g          154 t
ORIGIN      
        1 atcggttttt tttgaaaatg tttaggtaaa aaaccatcaa ttcctttatt tctttgaaca
       61 atttttgcct gtaataattt aagaatgttt tcctcttcct cagttttggt tctatatgct
      121 tcgattcctt tattcaataa atttgaactt gtttttataa cattttccag acgttgctga
      181 gttatattga tagcttcctc agataaatca atccccatat aatttctatt caaaatcttg
      241 gaggctacta aagtagttcc acttccacag aacgggtcta aaactatatc atttttatca
      301 gtagcaatct ttataatttg ttctaataac agaataggtt tttgtgtagg ataacctact
      361 ctttctttag c
//
LOCUS       DAVKWS010000156          367 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKWS010000156 DAVKWS010000000
VERSION     DAVKWS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.164"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            128..>367
                     /locus_tag="VW852_002154"
     CDS             128..>367
                     /locus_tag="VW852_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273199.1"
                     /translation="MHTLLDLFDWFTIIDRPKICLIGSRVWFEKRNLFYIHLDYRILA
                     LNTAENLESGNSSIVPVKDGKQILIDQLFDGECDDI"
BASE COUNT          136 a           43 c           58 g          130 t
ORIGIN      
        1 tttaactcaa aaaataaaaa actcaactca tatacaagtg caagaacgtt tacaattgtt
       61 gtctctatat cctactctaa aaaactttgc aaagtatgat tgtatatttg ttgatgatga
      121 agatttgatg cacacgctat tagacttatt tgattggttt actataattg atagaccaaa
      181 aatatgtttg ataggttcac gtgtttggtt cgaaaaaaga aatctttttt atatacattt
      241 agattataga atattggcac tcaatactgc cgaaaattta gaaagtggaa atagttcaat
      301 tgtaccagtt aaagatggca aacaaatttt aattgatcaa ttatttgatg gagaatgtga
      361 tgatatt
//
LOCUS       DAVKWS010000157          335 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKWS010000157 DAVKWS010000000
VERSION     DAVKWS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.165"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            127..>335
                     /locus_tag="VW852_002155"
     CDS             127..>335
                     /locus_tag="VW852_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0273200.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQ"
BASE COUNT          101 a           67 c           56 g          111 t
ORIGIN      
        1 ccttgcctta gcttagatcc tggatggttt cttttttcac ccaatgggtg ttttttacta
       61 gacaaaaaag agtttcccct ttatggtata agtgtagaaa aaaacacaaa aagaaaggaa
      121 actcacatga acagtttacc aaatcatcac ttccaaaaca agtcttttta ccaactatct
      181 ttcgatggag gtcatttaac ccagtatggt ggtcttatct tttttcagga acttttttcc
      241 cagttgaaac taaaagagcg gatttctaag tatttagtaa cgaatgacca acgccgctac
      301 tgtcgttatt cggattcaga tatccttgtc cagtt
//
LOCUS       DAVKWS010000158          327 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKWS010000158 DAVKWS010000000
VERSION     DAVKWS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.denovo.167"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            <1..>327
                     /locus_tag="VW852_002156"
     CDS             <1..>327
                     /locus_tag="VW852_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0273201.1"
                     /translation="TYREALITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKV
                     VVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLS
                     KISEPEA"
BASE COUNT          104 a           60 c           63 g          100 t
ORIGIN      
        1 aacttatcgt gaagccttga tcacgcgtga aaagtcattt ccaactggtt tagatatgga
       61 atttctagga aaggacttgc caaatgtagc gattcctcac acagatattg ttcataattt
      121 ggctgaaaaa gttgtggtcg ttcgattaga gaaaccggtg actttccata atatgattgc
      181 gccagataag gaagtagaag tatccttgct cttctttatc attaataatt caagttcaag
      241 tcaaacgaac attcttgctc agttaatgga cttcttcaca ggaaatggtc atcttgaaga
      301 cctatcaaaa atttctgaac cagaagc
//
LOCUS       DAVKWS010000159        12672 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00037279
            SAMN33606934-rid17948183.guided.054, whole genome shotgun sequence.
ACCESSION   DAVKWS010000159 DAVKWS010000000
VERSION     DAVKWS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606934
            Sequence Read Archive: SRR23726242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722119.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722119.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:06:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 1,996
            CDSs (with protein)               :: 1,996
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 51 of 123
            Pseudo Genes (incomplete)         :: 61 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606934-rid17948183.guided.054"
                     /strain="AUSMDU00037279"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST63"
     gene            86..823
                     /gene="erm(B)"
                     /locus_tag="VW852_002157"
     CDS             86..823
                     /gene="erm(B)"
                     /locus_tag="VW852_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV0273202.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(1080..2327)
                     /locus_tag="VW852_002158"
     CDS             complement(1080..2327)
                     /locus_tag="VW852_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0273203.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            2507..2728
                     /locus_tag="VW852_002159"
     CDS             2507..2728
                     /locus_tag="VW852_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0273204.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            2845..3342
                     /locus_tag="VW852_002160"
     CDS             2845..3342
                     /locus_tag="VW852_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV0273205.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3317..3823
                     /locus_tag="VW852_002161"
     CDS             3317..3823
                     /locus_tag="VW852_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV0273206.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            3807..6254
                     /locus_tag="VW852_002162"
     CDS             3807..6254
                     /locus_tag="VW852_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0273207.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6257..8434
                     /locus_tag="VW852_002163"
     CDS             6257..8434
                     /locus_tag="VW852_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV0273208.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8431..9432
                     /locus_tag="VW852_002164"
     CDS             8431..9432
                     /locus_tag="VW852_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV0273209.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9429..10364
                     /locus_tag="VW852_002165"
     CDS             9429..10364
                     /locus_tag="VW852_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV0273210.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10609..10725
                     /locus_tag="VW852_002166"
     CDS             10609..10725
                     /locus_tag="VW852_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV0273211.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            10741..12660
                     /gene="tet(M)"
                     /locus_tag="VW852_002167"
     CDS             10741..12660
                     /gene="tet(M)"
                     /locus_tag="VW852_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV0273212.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         4063 a         2285 c         2610 g         3714 t
ORIGIN      
        1 ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag
       61 atgtaatcac ttcaggagag attacatgaa caaaaatata aaatattctc aaaacttttt
      121 aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac
      181 cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa
      241 acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa
      301 actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa
      361 acagaggtat aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa
      421 agtggttttt gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa
      481 gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca
      541 gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt
      601 aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta
      661 ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca
      721 tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc attacttatg agcaagtatt
      781 gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt
      841 ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta gatatactac
      901 tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag
      961 gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgggata
     1021 tctgtcaact ggtatggttg actaaaaata cttcctacga aaatgtaggg ggtatttttt
     1081 tacgaaaaaa tacaatcgat tcttaaaaag aaaaattttt gattggcaaa accataacaa
     1141 gttcgtttta gggttttgat tttgcgattg atgccttcta aaggaccatt agagtattca
     1201 aatttagcgc tatttaagac atattttctg ttttgacgaa gggtttgaat agcagtatcc
     1261 atttctgtat tggttttttg gtagtctaag atggttgact ctagtaattc actattgcgc
     1321 tcgtttaggg ctttcgtgat atcttggtaa gtttggtata cttcagcgaa cttggaaaat
     1381 ttactagtaa tgagatcaac agcattttgg cgagtcatat attgtttaac gccgcgaaga
     1441 aaaactactt cttcagggtg aagatcttca gcttttttat ggaatagctt ccaatgtgac
     1501 ttcataattt tatattcttg gctctgttta tcaagttgct ttaggataga gatacgacaa
     1561 ttgtccaaag cgcgaccagc taattgtaca aggtggaagc gatcaataat gatattggca
     1621 ttagggaaaa ggcgatagat aaaactttga tattgagcat ttaaatcaat tacaactgat
     1681 tgaacgcatt cgcgttcggc ttttgaataa cgactttcaa aataatcaac aatggtaggt
     1741 gatagacgat cctgtaactt tgtgacaatt tggtgggttt cagcgtcaca acagataaag
     1801 gacatcacag acttaattga acgaaactcg tcaaaacata gatgcttagg caacttagcc
     1861 acacgatagt gtggttccat gcgctctaag attgttcgac gaacactgct aggagagcag
     1921 tgacacattt cagcaataag ctgaccagat aagcctttac gagctaaaag catgatttga
     1981 tttttgagat cactggataa ggtttgattt tctttggtta aattagtaat agcaccaaaa
     2041 gtagtatggc atgatttaca tttatagcgt tgtttacgaa gctctagttc atatcttctc
     2101 ccatttaaac ttgccagtcg tacatgagtt ttgcgaaagc catccttatt aactgtggga
     2161 aagccacagt tacgacaacg attaatcgga taagaaagag tagctgttat tagcgttata
     2221 tactctttaa cagaatcgtt gttgtgttca gcttcttcaa cagaaataat tttaatattt
     2281 ttatctttaa ttccaagaat atttaggata gaatcattat gggacatttg tttaaccttc
     2341 tttcatgatt tttgtggtga attgattgta taacgagggg acagcaaatg tcctcttttt
     2401 tgtataaaaa aatctggcat ggaatctcta tccataccag aaagtgtata ccccaaaaaa
     2461 ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga attttggaca
     2521 aaacctttat aactggtttc tatcaaacgc tcaatcactg gtgcttttag caatcgttgt
     2581 gattggcttg tatcttggct tcaagcgtga gtttagcaaa ctgattggct ttttaattat
     2641 tgcgattatt gcggttggct tagtcttcaa cgctgctgga gtaaaagaca ttttactaga
     2701 gctattcaat cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa tataagtggt
     2761 tttcttatgt tccgcaaagg aatggtacac caaacgaagt gcggtaggga tttttgaatc
     2821 tctacaaaga aaggacgtga atatatggac gatatgcaag tctatattgc gaatttaggc
     2881 aaatacaatg agggcgaatt ggtcggtgcg tggtttacct ttcccattga ctttgaggaa
     2941 gtcaaagaga aaatcggctt gaatgatgaa tatgaggaat acgccattca tgactacgag
     3001 ttacccttta cggttgacga atacacttcc attggcgaac tcaatcgact atgggaaatg
     3061 gtatcggaat tacccgaaga attacaatcg gagctatctg ctctgctcac tcatttttca
     3121 agcattgaag aactaagcga acatcaagag gatattatca ttcattccga ttgtgatgat
     3181 atgtatgacg tggcacgcta ctacattgaa gaaacgggtg ctttaggcga agtaccagct
     3241 agtcttcaaa actatattga ttatcaagcc tatggtcggg atttagacct ttcaggaacg
     3301 tttatctcaa ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg gtacattact
     3361 actggcagat tttctatttt acggggtggc tcaatcagct acccctattt tttatgaaag
     3421 gattgattac atgaagaaaa tacgaagcta taccagtatc tggtctgtgg aaaaggtact
     3481 gtattctatc aatgatttta gacttccgtt tcccataacc tttacgcaaa tgacatggtt
     3541 tgtcgtgtca ctctttgcag tgatgatact tggcaacttg ccccctcttt ccatgataga
     3601 gggagcattt ctcaaatact ttgggattcc tgtggctttc acatggttta tgtctacaaa
     3661 aacttttgat ggtaaaaagc cttatggatt tttgaagtct gtcattgctt atgcactgcg
     3721 accaaagctg acctatgcag gaaaaaaagt aacgcttggc agaaaccagc cacaagaagc
     3781 cattacagca gttaggagtg aattttatgg catatccaat taaatacatt gaaaacaatc
     3841 tcgtctggaa taaagacggg gaatgttatg cttactatga gcttgttcct tacaattact
     3901 catttctaag tccagaacag aaaatacaag tgcatgattc tttcagacag cttatcgcac
     3961 aaaatcgtga tggcaaaatt catgctttac aaatcagtac agaatccagc atacgttctg
     4021 cacaagagcg ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc tatgacaaaa
     4081 tcgaccaaca gacagacgct ttaatatcca tgattggcga aaatcaagtg aactaccgtt
     4141 tctttatcgg ctttaagttg cttctcaacg atcaggagtt ttctatgaaa agtcttaccg
     4201 ttgaagcaaa aaatgctttg tctgattttg tctatgatgt gaaccataag ctgatgggcg
     4261 attttgttag tatgagtaat gatgaaatcc tgcgttttca gaagatggaa aagctcttag
     4321 aaaataaaat ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac ttcggctatc
     4381 tgattgaaca cctttacgga cagacaggca ctgcctatga agagtatgag taccatctat
     4441 caaagaaaaa gctggataat gaaacgctga ttaaatacta tgacttgatt aagcctactc
     4501 gctgtttggt ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat gaaaccgtct
     4561 atgtagctta ctttaccatt aacagcattg tcggagaact ggacttcccg tcctctgaaa
     4621 tcttctacta ccagcaacag caatttacat tcccgattga tacgtcaatg aatgtggaaa
     4681 ttgtagcgaa tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa ctgaaagact
     4741 tggataacca cgcttggcaa agtgataatg aaaccagctc caatgtggcg gaagctctgg
     4801 aaagtgtgaa tgagctggaa accaatttag accaaagcaa ggaatctatg tacaagctgt
     4861 cttatgtggt aagggtatca gcaaatgatc ttgacgaact caaacgtcgt tgtaatgaag
     4921 tgaaagattt ttatgacgat ttaagcgtaa aactggtacg accatttggg gatatgctcg
     4981 gcttacatga agaattttta cctgccagca agcgttatat gaatgattat attcaatacg
     5041 tgacctctga tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg ggggaaaatg
     5101 aggggattta tgttggctac agcttagata ctggacgcaa tgtctatctg aaacctgctc
     5161 ttgccagtca aggggttaag ggttcagtaa ccaatgcgtt agcgtcggct tttgttggtt
     5221 cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgcg gtgctttatg
     5281 gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa gaaaccttgc
     5341 cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa aacaaaggct
     5401 tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg gctattgata
     5461 ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca atccttagaa
     5521 aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg attgaggaat
     5581 tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa agttttacag
     5641 actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct atcagcttag
     5701 aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag gaaacttcct
     5761 ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc attagtacct
     5821 ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat ttagacgaag
     5881 catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg gttcgggctg
     5941 gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac ctcttagatg
     6001 aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac cttaacgaga
     6061 ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat cagaagcgat
     6121 tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt gtcggtgtga
     6181 tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga ccacctgtgc
     6241 gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa actggacgct
     6301 gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg ccatttttct
     6361 tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata cggtcaatgt
     6421 ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt atgtggataa
     6481 tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg tcatgtatgg
     6541 actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca atgcgacagg
     6601 ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag attccattgg
     6661 taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag agggtttcta
     6721 tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct atacgggact
     6781 gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc tggtgtttat
     6841 cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca atgacttttc
     6901 atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc cccattccga
     6961 tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca tacaggttca
     7021 gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg gtattgaccg
     7081 tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca gagaaaaaat
     7141 tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa agaccattaa
     7201 ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca tatttgtatt
     7261 cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg ctatgtttct
     7321 gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa aacgagccat
     7381 aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta ttacgacagc
     7441 atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct ttttgattgc
     7501 ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt taatgagtat
     7561 gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga gaaaacctcg
     7621 tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt ccatgactac
     7681 tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt cggggagttc
     7741 tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa cacttggaaa
     7801 acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg tagacactgc
     7861 tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg cagtatatca
     7921 aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt ctcaaaccaa
     7981 agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa ccattgcgaa
     8041 gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt ctgttcatga
     8101 aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac agtctaatat
     8161 tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag ttaagtccga
     8221 tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa ccgttcaaaa
     8281 gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca caaaagaacg
     8341 tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac caatcaaaac
     8401 cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt gattggtggt
     8461 tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt attttcagat
     8521 gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc cgcagaagtg
     8581 ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga agaatatgtc
     8641 aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag
     8701 tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa
     8761 ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa
     8821 tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga
     8881 aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa
     8941 gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc
     9001 aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat
     9061 acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt
     9121 ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa
     9181 gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca
     9241 ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct
     9301 tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca
     9361 atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga
     9421 atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa
     9481 aacagatacc aaaggaaaag aaacctcgtg tctactataa ggtcaatcct cataaaaagg
     9541 ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata ttcaagcact
     9601 ttacagctat agatactcat actattcacg aaacaactat catagaaaag gaatacgttg
     9661 atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat tcatgggagc
     9721 aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg acagatgaac
     9781 ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct tctgtaagag
     9841 gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta acctacagtg
     9901 tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct tatatagtga
     9961 gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc attaccaaca
    10021 tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg gttgattcca
    10081 ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga
    10141 cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt
    10201 acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa gtcacggtat
    10261 cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt
    10321 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
    10381 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tccttgacaa
    10441 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
    10501 aaggtattct taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt
    10561 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
    10621 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
    10681 aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac
    10741 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
    10801 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
    10861 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
    10921 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
    10981 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
    11041 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
    11101 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
    11161 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
    11221 tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
    11281 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
    11341 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctgttta tcacggaagt
    11401 gcaaaaaaca atatagggat tgataacctt atagaagtga ttacgaataa attttattca
    11461 tcaacacatc gaggtcagtc tgaactttgc ggaaaagttt tcaaaattga gtattcggaa
    11521 aaaagacagc gtcttgcata tatacgtctt tatagtggcg tactgcattt gcgagattcg
    11581 gttagaatat cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt
    11641 gaattatgta aaattgataa ggcttattcc ggggaaattg ttattttgca gaatgagttt
    11701 ttgaagttaa atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa
    11761 aatcccctcc ctctgctgca aacgactgtt gaaccgagca aacctcaaca aagggaaatg
    11821 ttacttgatg cacttttaga aatctccgac agtgacccgc ttctgcgata ttatgtggat
    11881 tctgcgacac atgaaatcat actttctttc ttagggaaag tacaaatgga agtgacttgt
    11941 gctctgctgc aagaaaagta tcatgtggag atagaaataa aagagcctac agtcatttat
    12001 atggaaagac cgttaaaaaa agcagagtat accattcaca tcgaagttcc accgaatcct
    12061 ttctgggctt ccattggtct atctgtagca cagcttccat tagggagcgg agtacagtat
    12121 gagagctcgg tttctcttgg atacttaaat caatcgtttc aaaatgcagt tatggagggg
    12181 atacgctatg gctgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
    12241 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
    12301 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
    12361 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa
    12421 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
    12481 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
    12541 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
    12601 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
    12661 tgtattttat gt
//
